LOCUS       DACCYH010000001        80588 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.001, whole genome shotgun sequence.
ACCESSION   DACCYH010000001 DACCYH010000000
VERSION     DACCYH010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 80588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.001"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(62..1540)
                     /locus_tag="IVY85_000001"
     CDS             complement(62..1540)
                     /locus_tag="IVY85_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8043050.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(1838..2773)
                     /gene="murB"
                     /locus_tag="IVY85_000002"
     CDS             complement(1838..2773)
                     /gene="murB"
                     /locus_tag="IVY85_000002"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP8043051.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2846..3766)
                     /locus_tag="IVY85_000003"
     CDS             complement(2846..3766)
                     /locus_tag="IVY85_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8043052.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3879..4631
                     /gene="xth"
                     /locus_tag="IVY85_000004"
     CDS             3879..4631
                     /gene="xth"
                     /locus_tag="IVY85_000004"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP8043053.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5495..6028
                     /locus_tag="IVY85_000005"
     CDS             5495..6028
                     /locus_tag="IVY85_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP8043054.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(6518..7042)
                     /locus_tag="IVY85_000006"
     CDS             complement(6518..7042)
                     /locus_tag="IVY85_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8043055.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(7035..7508)
                     /gene="tsaE"
                     /locus_tag="IVY85_000007"
     CDS             complement(7035..7508)
                     /gene="tsaE"
                     /locus_tag="IVY85_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP8043056.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(7631..8611)
                     /gene="pta"
                     /locus_tag="IVY85_000008"
     CDS             complement(7631..8611)
                     /gene="pta"
                     /locus_tag="IVY85_000008"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP8043057.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(8764..9444)
                     /locus_tag="IVY85_000009"
     CDS             complement(8764..9444)
                     /locus_tag="IVY85_000009"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8043058.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            9570..10439
                     /locus_tag="IVY85_000010"
     CDS             9570..10439
                     /locus_tag="IVY85_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8043059.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            10465..10980
                     /locus_tag="IVY85_000011"
     CDS             10465..10980
                     /locus_tag="IVY85_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043060.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(11027..11590)
                     /locus_tag="IVY85_000012"
     CDS             complement(11027..11590)
                     /locus_tag="IVY85_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8043061.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(11645..11824)
                     /locus_tag="IVY85_000013"
     CDS             complement(11645..11824)
                     /locus_tag="IVY85_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043062.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(11901..12410)
                     /locus_tag="IVY85_000014"
     CDS             complement(11901..12410)
                     /locus_tag="IVY85_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043063.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(12506..12886)
                     /locus_tag="IVY85_000015"
     CDS             complement(12506..12886)
                     /locus_tag="IVY85_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8043064.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            13216..13965
                     /locus_tag="IVY85_000016"
     CDS             13216..13965
                     /locus_tag="IVY85_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP8043065.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(14149..15426)
                     /locus_tag="IVY85_000017"
     CDS             complement(14149..15426)
                     /locus_tag="IVY85_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043066.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(15505..16149)
                     /gene="pcp"
                     /locus_tag="IVY85_000018"
     CDS             complement(15505..16149)
                     /gene="pcp"
                     /locus_tag="IVY85_000018"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP8043067.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(16348..17292)
                     /locus_tag="IVY85_000019"
     CDS             complement(16348..17292)
                     /locus_tag="IVY85_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP8043068.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(17294..17980)
                     /locus_tag="IVY85_000020"
     CDS             complement(17294..17980)
                     /locus_tag="IVY85_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP8043069.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            18454..18774
                     /locus_tag="IVY85_000021"
     CDS             18454..18774
                     /locus_tag="IVY85_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP8043070.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            18928..19833
                     /locus_tag="IVY85_000022"
     CDS             18928..19833
                     /locus_tag="IVY85_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8043071.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            19830..20603
                     /locus_tag="IVY85_000023"
     CDS             19830..20603
                     /locus_tag="IVY85_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8043072.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(20784..22208)
                     /locus_tag="IVY85_000024"
     CDS             complement(20784..22208)
                     /locus_tag="IVY85_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8043073.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            22419..22865
                     /gene="nrdI"
                     /locus_tag="IVY85_000025"
     CDS             22419..22865
                     /gene="nrdI"
                     /locus_tag="IVY85_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP8043074.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(22930..24912)
                     /locus_tag="IVY85_000026"
     CDS             complement(22930..24912)
                     /locus_tag="IVY85_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8043075.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(24926..25822)
                     /gene="murQ"
                     /locus_tag="IVY85_000027"
     CDS             complement(24926..25822)
                     /gene="murQ"
                     /locus_tag="IVY85_000027"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP8043076.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(25825..26886)
                     /locus_tag="IVY85_000028"
     CDS             complement(25825..26886)
                     /locus_tag="IVY85_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8043077.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(27097..27624)
                     /locus_tag="IVY85_000029"
     CDS             complement(27097..27624)
                     /locus_tag="IVY85_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8043078.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(27691..29193)
                     /locus_tag="IVY85_000030"
     CDS             complement(27691..29193)
                     /locus_tag="IVY85_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP8043079.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(29451..30572)
                     /locus_tag="IVY85_000031"
     CDS             complement(29451..30572)
                     /locus_tag="IVY85_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8043080.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            30698..31639
                     /locus_tag="IVY85_000032"
     CDS             30698..31639
                     /locus_tag="IVY85_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8043081.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(31789..32685)
                     /locus_tag="IVY85_000033"
     CDS             complement(31789..32685)
                     /locus_tag="IVY85_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP8043082.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            32954..34294
                     /locus_tag="IVY85_000034"
     CDS             32954..34294
                     /locus_tag="IVY85_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8043083.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            34512..34955
                     /locus_tag="IVY85_000035"
     CDS             34512..34955
                     /locus_tag="IVY85_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP8043084.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(35459..36877)
                     /gene="nhaC"
                     /locus_tag="IVY85_000036"
     CDS             complement(35459..36877)
                     /gene="nhaC"
                     /locus_tag="IVY85_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8043085.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(37280..38503)
                     /locus_tag="IVY85_000037"
     CDS             complement(37280..38503)
                     /locus_tag="IVY85_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP8043086.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(38534..39373)
                     /locus_tag="IVY85_000038"
     CDS             complement(38534..39373)
                     /locus_tag="IVY85_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP8043087.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(39390..40070)
                     /locus_tag="IVY85_000039"
     CDS             complement(39390..40070)
                     /locus_tag="IVY85_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8043088.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(40072..41145)
                     /locus_tag="IVY85_000040"
     CDS             complement(40072..41145)
                     /locus_tag="IVY85_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8043089.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(41792..42463)
                     /locus_tag="IVY85_000041"
     CDS             complement(41792..42463)
                     /locus_tag="IVY85_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8043090.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(42550..45228)
                     /gene="mgtA"
                     /locus_tag="IVY85_000042"
     CDS             complement(42550..45228)
                     /gene="mgtA"
                     /locus_tag="IVY85_000042"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP8043091.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            45887..46324
                     /gene="ndk"
                     /locus_tag="IVY85_000043"
     CDS             45887..46324
                     /gene="ndk"
                     /locus_tag="IVY85_000043"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP8043092.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(46346..47212)
                     /locus_tag="IVY85_000044"
     CDS             complement(46346..47212)
                     /locus_tag="IVY85_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8043093.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(47372..49465)
                     /locus_tag="IVY85_000045"
     CDS             complement(47372..49465)
                     /locus_tag="IVY85_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8043094.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(50214..50837)
                     /locus_tag="IVY85_000046"
     CDS             complement(50214..50837)
                     /locus_tag="IVY85_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043095.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(50996..52393)
                     /locus_tag="IVY85_000047"
     CDS             complement(50996..52393)
                     /locus_tag="IVY85_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8043096.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(52425..53396)
                     /locus_tag="IVY85_000048"
     CDS             complement(52425..53396)
                     /locus_tag="IVY85_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8043097.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            53770..54195
                     /locus_tag="IVY85_000049"
     CDS             53770..54195
                     /locus_tag="IVY85_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043098.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(54683..55384)
                     /locus_tag="IVY85_000050"
     CDS             complement(54683..55384)
                     /locus_tag="IVY85_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8043099.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(55400..55840)
                     /locus_tag="IVY85_000051"
     CDS             complement(55400..55840)
                     /locus_tag="IVY85_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP8043100.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(55840..56415)
                     /locus_tag="IVY85_000052"
     CDS             complement(55840..56415)
                     /locus_tag="IVY85_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP8043101.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(57263..57742)
                     /locus_tag="IVY85_000053"
                     /pseudo
     CDS             complement(57263..57742)
                     /locus_tag="IVY85_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(57901..58602)
                     /locus_tag="IVY85_000054"
     CDS             complement(57901..58602)
                     /locus_tag="IVY85_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8043102.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(58710..60119)
                     /locus_tag="IVY85_000055"
     CDS             complement(58710..60119)
                     /locus_tag="IVY85_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP8043103.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(60167..62032)
                     /locus_tag="IVY85_000056"
     CDS             complement(60167..62032)
                     /locus_tag="IVY85_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8043104.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(62153..63003)
                     /locus_tag="IVY85_000057"
                     /pseudo
     CDS             complement(62153..63003)
                     /locus_tag="IVY85_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            complement(63313..64026)
                     /locus_tag="IVY85_000058"
     CDS             complement(63313..64026)
                     /locus_tag="IVY85_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8043105.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(64094..64420)
                     /locus_tag="IVY85_000059"
     CDS             complement(64094..64420)
                     /locus_tag="IVY85_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8043106.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(64543..64770)
                     /locus_tag="IVY85_000060"
     CDS             complement(64543..64770)
                     /locus_tag="IVY85_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8043107.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(65092..66345)
                     /locus_tag="IVY85_000061"
     CDS             complement(65092..66345)
                     /locus_tag="IVY85_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8043108.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            66406..67146
                     /locus_tag="IVY85_000062"
     CDS             66406..67146
                     /locus_tag="IVY85_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8043109.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(67232..67990)
                     /locus_tag="IVY85_000063"
     CDS             complement(67232..67990)
                     /locus_tag="IVY85_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8043110.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(67987..69015)
                     /locus_tag="IVY85_000064"
                     /pseudo
     CDS             complement(67987..69015)
                     /locus_tag="IVY85_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(69035..72202)
                     /locus_tag="IVY85_000065"
     CDS             complement(69035..72202)
                     /locus_tag="IVY85_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043111.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(72426..73310)
                     /locus_tag="IVY85_000066"
     CDS             complement(72426..73310)
                     /locus_tag="IVY85_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8043112.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(73405..73842)
                     /locus_tag="IVY85_000067"
     CDS             complement(73405..73842)
                     /locus_tag="IVY85_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043113.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(74059..74970)
                     /locus_tag="IVY85_000068"
     CDS             complement(74059..74970)
                     /locus_tag="IVY85_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8043114.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(75427..75855)
                     /locus_tag="IVY85_000069"
     CDS             complement(75427..75855)
                     /locus_tag="IVY85_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043115.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(75883..76521)
                     /locus_tag="IVY85_000070"
     CDS             complement(75883..76521)
                     /locus_tag="IVY85_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8043116.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(76525..78561)
                     /locus_tag="IVY85_000071"
     CDS             complement(76525..78561)
                     /locus_tag="IVY85_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP8043117.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            78687..79352
                     /locus_tag="IVY85_000072"
     CDS             78687..79352
                     /locus_tag="IVY85_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8043118.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
BASE COUNT        23465 a        16655 c        13741 g        26727 t
ORIGIN      
        1 tctttgaaaa aaacataaat cagctaataa atatgtgaga caaagctgcc atccataaat
       61 actagctgat tttttcagtt aaatgtctat ttttttctct gatgatggta tttccaaaat
      121 attcccagtc atctggagtc ggtggatttt tccaaattat ctgttcttta ttaaattttt
      181 caagtaaaca atcagataaa taaatattcg tacgagaagt gattcgactc tcaatcacaa
      241 tattgtaatt tttaaatcct ttcacttgag aaataatgta atcggtataa gcagatcctt
      301 gcgaaaaatc aatacagaca tgaatcggtg cttcaaaata tgataccgga caaataccga
      361 ccaataaaaa gagataatca taaaataagc gaatcatgaa acgttcatct tttcccaaga
      421 aatagcgttt attctcttgt atgacctttt ggattgctgt actgaatatt ggatacgtct
      481 cgaagaaaaa ctgtagctgc tttttagacg taaagctagt catctcaaag tcgaatatcc
      541 aattttttcg attgacatgc atcataccca tacgaagttc ttttcgtata tatagtcttt
      601 cttcttcaga gatttcaatc gaaaatggta attgatcaat caattccact gacatttttt
      661 gactaatcat gtggatatca tgaaaatgag cttccgcaag tccaaatgcg ccatctaatt
      721 caaattcttc ttccgtgatc aaaaacaaca tgatataaga aatctcatca atgatctgct
      781 ctttagggat atgaagaaaa gaaatatgtg ataaatcctt gatgatcttc attccttctg
      841 agctcttaaa gtattcatct gaaaagaaac gttttccgat tggttgattc ccataggccc
      901 tcaacaaact aatagtggtt agcaaattga atttgattcg ttttgttgca gacagtttta
      961 aattgaacag atattcaaaa tattgcacga tactctttgc ttcattcatg acgctttctc
     1021 taaatggcat agaaaaacca ttaaagaccc cgaaatatac cccaaataat acgttccgta
     1081 tgtttatttc ttccccaact aatatgtttt tctttaactt tatttgatgc tgttcaagaa
     1141 aagagactaa ttctcttctt tttacatagg ctcttgatat gcttaaaaaa tgattctctg
     1201 caaatttttc gatcgttact tttgaatcca cgatctccgt taacagtcga gcaatttttg
     1261 atcgttgaaa gtagattctt cttaaatttt tgatatccgt caaattcaga cgttcgacat
     1321 ggatctctgc ttcttctgaa atttggatct ctggctttgt tttgaattca gtaaattctt
     1381 tatttaattc tatgatataa tttcttgttt taaatttaga caaacctaat gtttcatata
     1441 acttaggcag cactacataa ttctttttct caaattctaa atatctagct agcagcagtt
     1501 tttctctatc ttcctcttct ataaggtaat ccaacttcat atcctacacc tcaaaaaatg
     1561 cctcatacca attacttgat atgcagatga aagttcatct tttcacctat actgatttta
     1621 tgatggatta tgcttgtttt ttcatcagtt attctcattt tttatctttt ttttgagaat
     1681 attgcaaaaa atggaaaaat ttatgaggaa atattctata ttttttgttt tattgtgtta
     1741 aaatgtacaa gatttgcgcc atttgcgtaa acaaccacaa aaaagagcag gacggccacc
     1801 gtcctgctct tttacttatt ttaagttcca ttacttatta attggctaaa cttctgcttg
     1861 tttctgatat tgcttgtttt tctccaatga tccgcacttc tgtttctaaa gacacatcaa
     1921 atttttcttt gattacttct tggatgtgcg caatcaattc aatataatct gtcgctgtcg
     1981 catgatcgat attgacgatg aatccagcat gtttttcaga gacttgtgca ccgccccatt
     2041 tcaggccttg caatcctgct tgttggatca gctgacctgt atagtgtcct actgggcgct
     2101 taaagacact tccgcatgaa gggtattcta atggctgttt tgattgacgc aattcggtca
     2161 actcatccat tcttgctttg attgcttcat aatcccctgt ttgcaaactg aatcgagctt
     2221 ccaatacgat agcacgtaat tcttggactt tactgtgacg ataagaaaat accatttcgt
     2281 cttttgtcaa agttttcaat gttccatctt caagcaaaag atccacttca gcaaaaacat
     2341 ctttgatctc tccgccatac gcacctgcat tcatataaac cgctccaccg acacttcccg
     2401 ggatgccgca agcaaattca aatccagtca atgattctgc caaagctgca taagtcgtat
     2461 caatcagtga ggcacctgct tccgcaatga ccatcgttcc tttgacatga atctgtttca
     2521 tttcagtcag catgatgact acaccgcgaa tcccgccatc ttgaacgatc agattgcttg
     2581 catttcctaa taccatccaa ggtatatcat ggattcggca ataatcgatg atttgcttta
     2641 cttcttcttg ttttttcggg aatgcaagga catcagctgg tccacctgtt ttcgtaaacg
     2701 tatagttcat caatggttca tcaaacaata tagtaatcgt ggctaagctt tcaactattt
     2761 cttctttatt cactgaggat tttcctttct tttctcaaac gtcctcatta ttctatcacg
     2821 cttaatacaa tcttgccagt cttgttcaaa gttgatgacg gtagtgatct ttaagcactt
     2881 cctcacataa ttcatgactt cttacaactt ctttttgttt aagcaattct gtttccgatt
     2941 tacctaaacg gatctgagaa aagaaggcct ctaccattgg ctcaaacccg cgtttgacta
     3001 gcgtgtttgt ccagtcgcca aattctttca ctttttttcc atcagcatct tgaatgacaa
     3061 gctgggtcag atcgtttact tcataagtac catttttact tgttacttga tatttttcta
     3121 tattagcgcc acttgctaaa tccatcgtta aaatagccgt ttgattttgt gtttctaatt
     3181 gtaaaatggc atattccaaa aattcctttg tttctcttat ctggtatttt gttctcaaaa
     3241 tgggttcatc taataaatac acagctgtat caataagatg taaaaacaaa tcataaagga
     3301 cgaactctgt cgtttctttt gccgctattc gatttttttg caggtggatc aaccgcttat
     3361 ctgggatacg tttcaattct tctaccatag gagcaaagcg ccggttgaaa ccgatcatca
     3421 aaattatttg gttcttatca gctaattctt gcaattcacg aacttcattt agatggacac
     3481 tcagtggttt gtcaatataa acatggatac cattttctag acattttttg gctaattcat
     3541 aatgtacttt tgttgctgcg tggatcatac atgcttcgat tttttcttct aaaagctcat
     3601 cgatcgtttc atatagatgt tggaacccgt actctttttg aattctttct tttgtttctt
     3661 tatttcttgt tgcaaaataa aaatcaagtg ttccttgttt tttcgcataa gttggtaaat
     3721 atgctttttc tgcgatattc cctacaccga ttacaccaat cttcatctac tttcctcctg
     3781 gatcatttag ttactttcat cttacaccat ttttttctaa aatgaggtac aatagtatcg
     3841 caaaggtaag gaaattaatt cgaaggagtg tgcttttatt gaaatttatc tcttggaacg
     3901 taaacgggct tcgtgccatc gtaaataaaa attttttaga agtttttcat gagttagacg
     3961 ccgatttttt ctgtctgcag gaaacgaaac tccaagcagg acaaattgat ttagatcttc
     4021 caggttatta tcaatattgg aattacgcgg aacgaaaagg ctattccggc acagcgattt
     4081 ttgctaaaaa accagcattg aatgccactt acggaatggg tatcgatatc catgatacag
     4141 aaggccgtct gattactttg gaatattccg atttctttct tgtaacctgt tacacgccaa
     4201 attctcaaag cgaattaaaa cgtttagatt accgattaga atgggaagaa gccttttata
     4261 attatttgga aaatctaaaa aaacaaaaac cagtcatcgt gtgtggggat ctaaatgtcg
     4321 ctcatcaaaa gattgatttg aaaaactgga aaacgaatca aaaaaatgct ggattcactc
     4381 cagaagaaag agcggctctt tctcgactgt tggataacgg gtttatcgat acattccgtt
     4441 atttttatcc aacccaggaa ggtgtctact cttggtggaa ctaccgattc aattcaagga
     4501 aaaacaatgc tggttggcgg atcgattact ttttgacgag caaagattta gagccaggac
     4561 tagctgatgc aaagatccat acggacatta tgggcagtga tcattgtcca gtagaattag
     4621 atttgaaata aagaggttgt gaaaaagtcg tttagtttcg agtattaaga aaaattttgt
     4681 gaaaatggct ttccacattt tgcataaatt tggcataata ccgagaaact ggcttttgaa
     4741 cactgtttat cgagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa
     4801 caaaaatagt tcctcatatt tttcgtttgg ttgggcttat taccgcagat acaagctttt
     4861 gaacaccgtt tattaagagg ttgtgaaaaa gctgcactgc atcaagtaat aagaacagcc
     4921 aaacaaaaat agttcctcat atttttcgtt tggttgggct tattaccgca gatgcaagct
     4981 tttgaacacc gtttattaag aggttgtgaa aaagctgcac tgcatcaagt aataagaaca
     5041 gccaaacaaa aatagttcct catatttttc gtttggttgg acttattacc gcagatgcaa
     5101 gcttttgaac accgtttatt gagaagttgt gaaatcagcg tcctgacctg agtattagag
     5161 aaacaaatgg caaaatagtt cctcatattt tgctattttt tgagctaata ccgagggtca
     5221 gctgattgaa caccgtttag tggagaagtt gtgaaaaagt cgcctacttt cgagtcgtaa
     5281 gaaatttttt tgaaaatggt tcctcacaac tttgcataaa tttgacttaa taccgagaag
     5341 tcatgatccc catctaactc caaatcacaa ggaatgagtt caaaaactag cttttctcaa
     5401 caaatattac ataaaaaatt ctatgttttt tgttaaattt atttgctttt ttcaaaagta
     5461 tgttacagta atttcccaag gaggttttac agaaatgaat tttgttgcca tggattttga
     5521 aactgccaat catcagcctt atagtgcttg ttctcttgcg ctagtcatgg taaaaaatag
     5581 ccagatcgtt gatgaattct atacattgat acagcctgag actccttttt tctggcgaaa
     5641 tgtccaaatt cacggaatcc atcaagaaga cgtacgcaat gcgccgaaat tcccagaagt
     5701 ctggcaaact atccaaccat attttcaaaa aaatcgttta gtcgttgcac ataatgctgc
     5761 ttttgataca aaggtcttag cgggatgctt agattattat catcttgaac agcctaatta
     5821 tctttcttta tgtacagtga aaagtagccg tcgcctgttt cctgaatttc caaatcatcg
     5881 attgaacact gtgtgcgaaa atttgaacat ccaattagat catcaccatg atgcattgga
     5941 agatagtcgt gcatgtgcgg aaatcctgct ttatcaagaa aaaaaattcg ggacagatcc
     6001 attgaaaaaa cttgtgacta taaagtaaaa gaatacttat gaaacacaca aagaggtcgt
     6061 gagcctgccg tttcactccg agtcacaagg agcaatttca aaaaatagct tctcatgttt
     6121 attaaatatt gtgactggtg accgaggagc tggcaggaga acaccgttta ttggagaggt
     6181 tgtgaaaaag ctgttctgca tcaagtaata agaacagcca aacaaaaata gcttctcata
     6241 tttttcattt ggttggactt attaccgcag atgcaagctt ttggacaccg tttaccaaga
     6301 ggttgtgaaa tcagcgtttt gacctgagta ttagagaaac aaacggcaaa atagttcctc
     6361 atattttgcc gttttttgag ctaataccga aggtcagctg attgaacacc gtttattcaa
     6421 aaaaaatgga ctgcgacacg acttttgtca cagtcccttc gtgtgttttt ctgccttctc
     6481 aaaaggatga cagtttctca ctcttttcct atttcttcta ttcaatcaaa taactcattt
     6541 tatatacatc taaaaattca cctagatctg ttcttgcccc tctaggcatg accgcttcga
     6601 tttggaagcc catttttcgg taaaggtgta cggctctttc attacgaact tgaacgtcaa
     6661 gctccaatcg aaaaagtaca cccatttcct tcgcccaact gatgatttct tctagcataa
     6721 gagtgcctag tcccattccc caatattcct gcagaatact gataccgact tctcccacgt
     6781 gagacaaacg aaattgactg tctgccttga cagatgctgt tccaataatc gtcccttcat
     6841 agattgcaag cagcaataaa ttattattgc tttcgtatat ataatccaac tcttcttcta
     6901 aggtttctgg tggtaacagg agttctgctt catccaaaac taaaaattct gtctccttat
     6961 taacttgtcc catcatcaaa agaatgtctt ttgcatctcc tggcactgct tcccgcaaga
     7021 tgatcccaac ttcttcactc atgattttca gcccattttt ctagaattcg ctgtttgaag
     7081 gtttcacttt gctccccgta tgagattagt ttcacatacc gcttttctaa atctgtatca
     7141 cttttttcta aaatcagttc caaataatcc tccggcagtg cttcttctaa tagatttccc
     7201 cattcaacaa cggaaagccc atcaccttca aaatattcat ctaaccctaa atctgctccg
     7261 ctagctgcta cccgataaat atccatatga tacaacggaa tccgaccttg actatattct
     7321 ctaataatcg tgtatgtcgg acttttgatc atttgcgtta tccctaatcc ttgggcaatt
     7381 cctttcgtca acgttgtttt acccgcacct agatcaccag tcaatatcag attgtcacct
     7441 ggcattgcgg aatgaccaat tgcctctgcg aattgttctg tcattgttaa tcctgatagt
     7501 tcgatcattt ttcatcttct cccgctcttc tttctttttg ccagtatatc gcgtaatgaa
     7561 gtttttttaa agaaaaaatg aactagtcaa gaacagcagc tgctgttttt ggctagttcg
     7621 cgtgtatttg ttaatcaatc aatgtttgtg ctgctgtgat aatcgataat ttatatacat
     7681 cttcttcatt tgctccacga gacaaatcag aaaccggttt gttcaagcct tgtagaattg
     7741 gtccgattgc ttcaaagtta ccgaaacgct gagcaatttt atagccgata tttcctgact
     7801 gcaattctgg aaatacaaag actgttgctt gaccagctac ttctgaatct ggcgctttca
     7861 gttgtgcaac tgaaggaaca taagaagcat caaattgcaa ttcgccgtcg atcatgtatt
     7921 gtggcgctag ttttttagcg attttagttg cttcaactac tttgtctact tctggcgatt
     7981 tcgctgagcc ttttgtcgag aagctcatca ttgctacttt tggttcgata tcgaacaatt
     8041 ctgctgtttt tgcagaatct acggcgattt cagccaattc ttgcgcagtt ggattcacgt
     8101 tgatcgcaca atccgaaaag acatattttt cttgatctct tccacgtacc atgatcatcg
     8161 ctccgcttgt ccgactgatg ccaggctttg ttttgatgat ctgaagtgct gggcgaacag
     8221 tgtcgccagt cgaatggatc gctccagaga ccattccgtc tgcaatcccc atatacgtaa
     8281 gcatcgtacc aaagtaattc acatctttta aaatcttttc tgcttgttct ttggatgcct
     8341 ttccatttct acgttcaaca aaagtatcca ccatttcatc ccacttatcg taattatcag
     8401 ggctgatgat cgtaaagcta gatgttttga ttcctctggc atgcgctgca tttcttattt
     8461 cttcttcgtt ccctactaaa atgggttcca caagttcttc tgctttcaac cgggcagctg
     8521 cgcctaaaat acgagcatca gtagcttccg ggaaaacgat tttgatccca cggcgaacaa
     8581 ttttaaatct taaactatca aacaattcca caagatgacc tccagacgtt tttatatgta
     8641 ttatcataca ctattccaga aaaaaagaac agtttatttt atctgtatca gtcaaaaatg
     8701 ctggaaaaat taagtttttc acatacaaag cgaactgcac tataacagga aagcgctgtt
     8761 ttattacact gtttcaggca gttgccaatc aatcggttct tctcctaacg caaggagcgc
     8821 atcattcgtt ttcgagaagg gtttggaacc aaaaaagcca cgacttgcag ataacggact
     8881 gggatgtgga gatgttatga tgatatggcg actcttatcg atcattttga ttttttcttg
     8941 tgccggtttt ccccacaaga taaacacgat tggtttttcc cgttcattta atttttcaat
     9001 gatcgtgtct gttaatcttt cccaaccttt cccgcgatga gaatacgctt gcccttcccg
     9061 aacggtaaga acggtgttga gtaaaaggac tccctgttta gcccatgaca ctagatttcc
     9121 atgttgaaca ggtgcgattc caagatcatc ataaagctct ttatagatat ttcttaacga
     9181 tggcgggatc tttacgccag gttgcacaga aaaagataag ccatgagctt gattttctcc
     9241 atgataagga tcttgtccta aaatcaccac tttaacttct tcataaggag tcaattccaa
     9301 tgcttcatag atatgataca tatctggatg gatcttttgg gacgcatatt ctttttttaa
     9361 aaattctctt agatgcagat aataatcttt tgaaaattct gaggacaaaa cttcttgcca
     9421 actgttatga atgatttctt tcaaaaactc cacctcctaa taaaaagaat aactcacatt
     9481 ttcttcaatc acaagggaca atcttcaccc tcagcacttg attaatatgg taaactagga
     9541 acaagaagca gaaaggaacg ataatcatta tgatcaaatt aattgcatca gatatggacg
     9601 gcacattatt agatgcccat atgagtgttt ctcctgaaaa tgccgaggcg atacgttttg
     9661 caaatgatgc cggtgttgag ttcatggtcg ctactggtcg aaactatcaa gaagctcgcg
     9721 cagctttaga tgaggtagga atcgattgtg cgatgatcac tttgaacggg gcacaggtat
     9781 ttgataaaga aggaaattca ttgtttaccg tgtcgattcc aaataatcaa gcaatctctg
     9841 ttttagatat tctagatgct aacggtattt actatgaagt agcaaccaac gatggtcttt
     9901 attctgaaaa tcaagcgaaa cgaattgaaa gttttgcttc catggtggct acccatttac
     9961 ctcaccttac gtataaaatg gcgattgcca tggcctcagc taagctggag ttattgcaca
    10021 tcacatacgt ggacagtatt cgtgctattt tggaagaaaa gaatctggaa gttttaaaaa
    10081 ttatttgttt tcatacagaa gggccagctg tattaggtcc agttggaaag cagatttctc
    10141 ttttaggcga tctagccgtt acttcctctg gtcaaaacaa tatcgaagtg aaccataaaa
    10201 acgctcaaaa aggaattgct gttgcccatg tcgctcacga gcgtgggatt tctttagaag
    10261 aagtcatgac gattggcgat aacttcaatg atgtcagcat gctgcaagta gcaggagtca
    10321 gtttcgcaat gggaaatgca gaaatcgaag taaaagatta tgccaaatat acaacggaca
    10381 caaaccttga atctggcgta ggaaaagcca tattgcgcgc gattgcagaa gacttgtagt
    10441 tttatctaga aaagagggat atagatgtca gaatttttca ttcaggaaaa gcagcttgga
    10501 cgtgcaactc ggacaattgt caaaaacgaa aaaggacatt ccctgttttt gatggttggt
    10561 cgctggggaa caaagggcga tgcattgtct ctttatgcca tgaatggcga tctcgttgct
    10621 cacatcaagc agataagcct gacattcggt acgcgttttg agctgtataa agagttcgaa
    10681 aaggtgggaa ccatgcagaa aatattcaat tggcccggtg acttttatta tattcgtaga
    10741 cttcactgga cagtccaagg tgatatctat aatcatcaat atcaaatcca tcatttcaat
    10801 cagcaaatca tgaaaatgga taaagcaaca cttttttcag gagattacta tgtattggat
    10861 gtctcaaatg atgaagatgc tccagtttgt atttgtattg ctgcggtttt agattactgg
    10921 ctctaccgta aagacaaaag tcgcgataag cctgtttctt accgacttaa cttaagttaa
    10981 aaagatgtac ctgaaatgcg gaaatacgca tttcaggtac atctttttag atattctttt
    11041 gcatatgatc ataaagatga ccactgatca tccgctgacc gacaactttg aagtcatggc
    11101 gttcgtataa ttttttagcc tttggatttt tttcatccac acttagaccg atgacggagc
    11161 gattagcttt cttcgccaat ttaggtaatg cttctaataa tcgcgaaccg atgccttgtc
    11221 ctctaaaatc ttctgataca ctgatagagt ccaaatacca ttcgttggga aacgtttctt
    11281 tatcaatgaa caaacgtgta gacgcatcta gctgatattt ctctaagata ggtaccagtg
    11341 gctgatcgat gattgattct tcttcatctg tataaccgta agcaactcct gcaactttac
    11401 cgtctatttc ttctacgata cctcgtgaat aactgtaacg ataatttgga tcagtgattg
    11461 cttcttctaa aatctctaaa gtcttttgtt cccctgtctg cttcaaaaat ggcagttcca
    11521 tatcttttag aatcactaag atcaatgggg cgatttctgg agcatcttct tttctcgcaa
    11581 aacgaatcat agtttcctct ccttttctcc attctaacat gcagaaaaag aaatcgcaaa
    11641 atcgttattt taaaaaatct tcaatctttt cagtaatgaa ataaccatga gtaacgattc
    11701 ttgccgcttc agttcggcaa taagggcaga cgactggcat ttctttatca ttatgttcga
    11761 ctacattatt gacttcttcg tatgcaaatt tttttccgca attagggcat ttcatttttt
    11821 ccatgttatc tcaacttcct ttcatcttta ctctcttagc ttactcttat attgtaaaaa
    11881 agagaagcaa tatccttctt ctatgcagat atccatacct gacggtcctg ttctccatct
    11941 attaatcgga ctttataata aggacgttca tctttgacta gaagtgtcca ctctttcact
    12001 tttgcttttt tcccgtagta gctgactgct tccttcatcg ctgcttgcgg ttgtttgaac
    12061 tcttccgttt tttctatatc aaaaagatgt tctgcggcct tttccgtgtc atttcgtacg
    12121 gttgttttcc cagtggatgg attgactgct atgacttgtc cttcacaaaa aaactggtat
    12181 tcatatgttg cattcgtagg attctccaaa tctttttcaa ctgcaaaatg aatagcagat
    12241 atgttatgtt cttttgtttc gttttttacg attgctgctg cttttttcaa tgagatccga
    12301 tattcctggt cactaatatc atccatgtga aaagcggcat tgagcagtcc cgtcataccg
    12361 gtaatcccta ttacgatgag ggcagcaaca ccaaacatca tttttttcat tcttcattct
    12421 cctgtttctt ttttttaaag aatatcaaga aatgaacgaa tgaaaaatcg aatgaaacga
    12481 aaaaaaagtg acaattttgt tatctttatt cgtgcagttc taatggcaat ccgtctggat
    12541 caaagaaaaa cgtcattttt tttcctgtaa aggtatcttc tctaatcggt tctgattcga
    12601 tccctaagga ctgcaattct gcaattgttt cttcaatatc gtctactttg aaagcaagat
    12661 gacgcaagcc acaagcttct ggatagtttg gtcgttgtgg cgcattagca attccaaaaa
    12721 tctccaattc agcacttcct agtttcagat ctaatttata gtcatctcgt tctttgcggt
    12781 aattttctct gattacttca aatcctaatt tattaacata aaaatcacgt gatttctgat
    12841 aatctgaaac aatgatggct atatgatgga tcgttgtaag gttcatatga gcactcctat
    12901 tcttcgttta agctcggatc tatattaatc tccagttcca ttgtaaatgt gtcagattga
    12961 aaaaaatagc tgtttttcct ttttataaga aaaaaggcaa aaaaaacaga aagtaggtcc
    13021 tccaagacac tcttcctgtt cccaatataa taaatgaaag taagaaatta tattttcaag
    13081 aaaacaaaca gaaaattatc ataacacaaa ttatttataa ttgaaagact agtttttttt
    13141 caagaaaagc atacaataga ttgtgttagt aaatacagaa cacactacat ttagtgtctt
    13201 aaggagtcct catacatgaa gtcatatttt cagtttttag ctcatcagct aaaaggctgg
    13261 ccgcaacaaa attattatct tttctttttc agtcttggct gccaggtcat gactcttgtc
    13321 aatagtccca tcacttggct gtccgttatt acatttatcg gtaccacgct tggtgtattg
    13381 tgtattttat ccatcaatgc ggcaaaatca gtcaacggta ttttaggtat tttgtctgcg
    13441 atttgtttta ttatcgttgg gttttcagca aagaactatt taagtatcgg tgaacagatt
    13501 gcttatatga tcacattgga tatccccgta ttgttaagta aagaatggaa tcataatatg
    13561 gcttcaaaaa tccgaaaatt cactggaaaa agctggttta tcgcaatcat cagtactttc
    13621 cttgtctatc ttgtttcagg attcctgatt ggtcgttata cagatgatcc tcggccgtgg
    13681 gtagatgcca ttgctttttc tattagcttg actgctggta tcatcagttt cttgcgttac
    13741 aacaaccaat attactggtg gttagcttca gggcttgctc aaatggtcct ttggtatatt
    13801 tcatttacgc atggatctgc aagtttggcg atgttcgtga acagctctgt ttacttgctg
    13861 aatgatgtcc ttgcatttac cgtttcacct tggtataacc aaaaagaacg tgaacgtatg
    13921 caaaaaatgg aagaagaata ctacaacgca caacaacacg cataaaaaaa ctgagcgtgg
    13981 gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtataaacgg cggaatcaga
    14041 agcaactcct tcggaaataa gctgaaattc acaaaaattt gaaaagcaat tttcgtgaat
    14101 ttcttcttat ttcttggagt taaacgcttc tgtcccagcc tctatttttt atagtaattc
    14161 cttcagatac tttcttgcct catcattgaa acgcagtgat tcttctaact tctgataata
    14221 ttccaacgct tgtttctgag cttcagtaaa acgataatgt tttcctttcc atttgtcgat
    14281 tgtctcttgc acatttccag attggatatc tttcactact agttgccatt tttcttttga
    14341 gactgtacgg tctttcttat cagcagattg tatagcttct cgttctttac gcaatacttt
    14401 taaaaaacca tttctgacta tccgaggaat gctgtcgaag aacccttctg taaaaccata
    14461 gcgacaatat tgcttccaaa taaatagata gatttttggt tggatgaatc gttggttttt
    14521 cgcaaaatat tccatctgag cagtgacttc tggtgttcgt tgacgttttg tctttctcaa
    14581 cacggaaata aatgtttcaa aataagcatt aggatgtact ggcacttctt catatacgat
    14641 ggattgccaa tctttacgta aaaatgcgcg atagttgatc aagaattctt ctacatcctt
    14701 tactaattct tctgcaaagt cgtcaaatag ttcttttttt atctgtcgct tttttgactg
    14761 cccattgatg tgttcactta ttttcttgat ttcattttcc aatgcttgat aatccgctat
    14821 gtaaatcggc atttctactt ggcgaaattc tgcgagccgc tgagctaatt ttatatccgg
    14881 taaatatggc tgtttcatcc gacgattttg gtacaagaca aaacagtatt tttgccatag
    14941 tccttcagga aaatcgatat tcttctgctt aagccataat aattcgtcta aggccagatc
    15001 aacttttgtc attccctgat ggatcgaagc tgccactgtc ggagagacat gcaggtgatc
    15061 ggcaaagtgg ttgatgccta gttttaggat ctttccggtt tttggcgatt taacgatgta
    15121 ttgcactttt aattctctac cacactcaca atacaatttt tgctggcttc gttttgttcg
    15181 aaaaaacgga ttgatcttga agtcagaaaa cacccaatct gttgcagcta aataatgttc
    15241 ttccataaaa cgagagctga ttagatatcg gcggtgctta tcgactagtt cccgctgttt
    15301 tttcgttaat tttgcataga tctcccaacg ttgactgctt gtgtattgtt tagaaatttt
    15361 gatgggatag cttttgctga tttcttttgg aaaccattct tcaaagacgg attgccattc
    15421 ttccacggct gtttctttcc tttctaatta caaaaagctg gctactaaca taacgttagc
    15481 aaccagccag tgtttcttgc taaattagtg agttgttccg gcatttccgg aaatatcttc
    15541 tttattttct actgctgctt caactgcata agtcaaagaa gaagcaatgt cttctaaaga
    15601 catagaaggc gtacccactg gacgatcgat cacttgatcg ggtgagaatg gcacgtggat
    15661 gaacccacct cgaattgccg ggaattgtgt ttcgatcaaa tgaagcaaac gatacatgat
    15721 atcattgcag acaaatgttc ctgctgtata agagatgtac gctggtagac catgatcttg
    15781 tacatttttt gtcattgctt tgattggcaa tgaggtaaag taagcggctg gaccgttttc
    15841 ttctaaagcc gtatcgaatg gctgatttcc ttcattatca gggattcttg cttctgctag
    15901 attgattgcg acacgttcga acgagactgc tgaccgccca cctgcttgtc cgacacagat
    15961 cacgacatct ggttggtgct gttggatggc ttctttgact acttgactgc ttttttcgaa
    16021 tacggtcggt acttccactt tgatgatttc agcaccgctg atttcatctg gcattttttt
    16081 tactgcttca taggctggat tcactttgtc cccaccaaat ggatcaaaac ctgttactag
    16141 tactttcatc tgttgttcct ccgtccgatt gatttaacat atggttatct tttatcatac
    16201 cattttcctt gaacagcgta aataaaggaa acaatagttt aaataacata acagatcaaa
    16261 cttaatatat tcttataaaa aaatagtgtt ccactacaat gttagaagag gttgcgacaa
    16321 ttattctgtc acaacccctc tcttctatta tttaaataga atcatataag caatttggaa
    16381 aactagcata aataacgcaa taaacaactg gtttttgatc actccgtatt tgtctttcat
    16441 ctccagcatc gctacaggca cgatattgaa gttagcagcc atcggtgtca gcagggtccc
    16501 acaaaaaccg caagtcaatg caaccattcc tattaacgct ggattcgctc catgactaaa
    16561 tacgaatggt gcaccgatcc caaccgtcat cacggtgatg gccgcaaatg cattccccat
    16621 gattaccgta aatatcacca tgccaagagc gtaaatgatg atgccgatgt tcacatttcc
    16681 ttctggaacg atcgtaccta ctgcattgga aatcactgtc ccaactccag cgcttgtaaa
    16741 taccgcacct agagaagcta aaagcattgg caacatgctt aacggcccta ctgtgcccag
    16801 catatctgct gcatcatcta aaaatgtact tggtcgatta tctcgagaaa agaacatcaa
    16861 gattagcatg gctgcaaaaa caccgacgcc cacgccgact aaagcaccta aattggtaaa
    16921 tagagcaaac aagatcgcaa agacaccgat actcaaagct ggaataaaaa ttttcattcc
    16981 tagtttgtct gacatttttt ccatatattc ttttgatgga agccggcttt ctcctttgct
    17041 gactttctta gcaattgctg gtatggtcat cgcaaagatc aagccaccgt tcacgattgc
    17101 cggaagccat cttcctcctg caataactac ccctaagacc gtccagaaaa aggctgaccc
    17161 ataacgatga ggattggttt tatctgttaa atttcttact ccagtgtaaa tcaatacgag
    17221 tcccatcacg atataaagga tctccagtaa tttatcgcct aataaaatat caggatttgt
    17281 aaaaaagtcc atcttattgc ccctttcctg gataatattt cttcgctaat ttcttttctt
    17341 taagatagaa gtagacactt gccacaacta aagccgtgat agccactgga acttccacta
    17401 aagcaagctg tcccaatgtt acgtcatatc ctagatcttt cagtgttgat tggactagca
    17461 acgcccctgc tcctccgatg aatagtactt ggccaaagaa ccagcagaca ttttccatag
    17521 ctgaagccat accttttagt tcttcttcat gcttaggatg gacttctaac ccttttgatt
    17581 cgatggtccc caatgccatc ggtaaaatga ttggacgaac aaatccagca actccaccaa
    17641 aactcacgtt aaaagccgca aagatcattc gaaagatccc ataaatatct acaatcatac
    17701 cagcagatac tttcttgaat cgcttgatca aggaagccgc tgattctttc agaccatttc
    17761 tttcaagcgt tcctgttgcc aacataatga tgataaaaat cgccatcccg cgattgttta
    17821 caaaacttgt acctaaaagt tctaacattc cttgaatgcc aagaccgcca gtcaaagctg
    17881 taacaactaa agcaaccatt acgatcaata tcgagtctaa tttaaatgca aacccgagaa
    17941 taacaatcaa gacgcctaaa aggctaagat aattttccat atattttcct ccataaaatg
    18001 acaatatttt tttaattata aagcaaaaca cataaaaaat atagttgttt tctcacgaaa
    18061 tagacactat ttttaaatat ttttcgttat ttttttatca caaaaattat cttacttatt
    18121 gtaaggtaaa gcttttttta atttattgcc cgataatata tagaaataag gtctagttac
    18181 tgtttttttt aaatgattaa taaataacaa cttctttcta tgaaattgca aagcattcat
    18241 cttcttttcc gctaacgata tatccgtcag tgcttggtac actttactaa aaagtatgag
    18301 attgccgata tctggatagg tgtgctatac tacagaaaaa gatatacaat ctgccaccgg
    18361 gtaggataaa gaggatgatc attccattag gtctttgaag ggatgatttt cctctttttg
    18421 tgtatttttt tatagttaag gaggaatgta agtatggcat ggatggaatt aatcatagca
    18481 ggaattttgg aagtattctg gtcaacgatg atgaaatgga gtgatggttt ttctaaaatc
    18541 aattattctt tttacacagt gattggcatg attgctagtt tttacttttt gtcaaaggca
    18601 atcaaaagtt tgccgatgag tctctcttat cctatctgga cagggattgg cgcagttggt
    18661 tcggtcataa ttggtgtggt cttgtttcat gataagctga atctttccac ttggttcttt
    18721 gtggggatgc tactcatcag tatcattggc atcaagatta ctagcggcca ctagacactt
    18781 ggaataagta ctgatcagaa aaaataagcc tttttattta atattcaatt tttttatgaa
    18841 aaaataagaa ataagtccaa tgacctagcg acttctccac tcatcaggtg tatgcttgta
    18901 ctatctatag aaaaggagtg aatatttttg aaaagtcttt cggtccaaga attatccaag
    18961 aactttggtc acaaacaagc cctcagcgat gtcagtttta gcttatcccc tggcaaaatc
    19021 gttggattag taggtcctaa tggcgctgga aaaacaacga tcatgaaagc tattttagga
    19081 ctgatccatt actcagatgg aacaatctgt attggtgaaa atgagatttc tccctctagt
    19141 cataaagggt tggaacaagt aggagcattg atcgaatacc cgggtatcta tccattctta
    19201 acaggctatg atcatctgaa gttattctct gaaaccaacg atgtatctgc tatcgacact
    19261 atcgtgaatc agctcaagat ggaaaagtac attcacaaaa aagcgaaatc ctactcgttg
    19321 ggaatgaaac aaaaattggg aattgctctt gccttactga accatccgtc tatcgttatt
    19381 ttagatgagc cgatgaacgg tttggatcca caagcaacaa gagatgtgcg aaaaatcatt
    19441 accgacttgg ctactcaggg cacctctttc ttgatctcca gtcacttact cagcgagctt
    19501 gaaaaaatgg tcgatgagct gatcctgatc aatcaaggaa agatcatcaa acaatgtaca
    19561 atggctgaat tgaaccaaac agcgcaagat ttcatcgtta tctctacaac aaatgatcta
    19621 gctgctcgaa cgatcttgac agaagctgga tatactttgg aagatcctga taaaatcaag
    19681 cttttaaaag aaaacgaaga attattagga accgtgatcc agctattgtc tgcacaaaat
    19741 atccatgtga cagacgtcca gcatctgacg aatgatttgg aatcttctct attggaaatt
    19801 ctggattcag taaaggggga agaagaaaaa tgacattgct tgtaaaacaa gaattattta
    19861 aattgatcaa gaaaaagtca actgctgttt tatctgttct attagttgtt ttgctcatag
    19921 gaacagcact tctagccaaa aaatacacga cgatcattga tccagttgaa atgactgctc
    19981 agctttttag tgccacatct tggattgtct ttatcatgat tgcagctgca agcacgatca
    20041 tttcgatgga agcacaatat ggtacattga aaaatctcct ttatcgaaag tattctcgag
    20101 gagaaatatt agtaagcaaa tggatcacat tagttattta ttctgtttat ttataccttt
    20161 tagctattat tgtcactgtg ttgatgaaac tgattctttt cccttcgatt tccttcacgg
    20221 agaaggtttc tactggtcaa acactgatcc aaagcttgtt cacttatact ttgggtagtt
    20281 atatcggttt atggctgatt ttaagtttag tgttgatgat tgcttgcttt atcaatagtt
    20341 ccggcgcatc catctcagca ggaatcgtct tttattttgc ttcgtcaatc atatctggta
    20401 tccttattgc actcatccaa aaatgggagt ggatcaaatg gaatccgatc agtatgttga
    20461 atctgcaaaa ccaaattgga aacgaagaga tcatgaagca attgactcat ctttctacta
    20521 atcaaatgct attcggtaat ttagcttaca tcgttttgtt cttagcatta ggatatctcg
    20581 tatttaagcg aaaaaatgta tgatgactca tcaaaaaaga ggctgtgaaa gagacgttca
    20641 gccttattgc cgattggctg tctcttaaac accgtttatt ctgaaaaagg agctgtgaca
    20701 acatttgtgt cacagctcct ttcctagtat gtttttgaat aaacgacagg ctcacaacct
    20761 ctctttatac ttcttttata cttctatacg tgaagcatcc cagtggcaaa taaaaccatt
    20821 gcaatcactc cgaccatcgt cacacctata agtaaaatct tatctaagtg gatatgttgt
    20881 acaggctcgt tttgctcctt acgtgctttg aggtaaaaat aaatgcctgg caagaaaaga
    20941 acaagaacaa gcattacttc attaaaggct acgactagca tagcccatac ttgaaaggca
    21001 cttgctaaaa gccctactaa aaattggacc cattctttcc gctgataaga aagtttcatt
    21061 tggtacaatc ctaagaaaag ccacgtgaag aaaatagctg ctgtaccaag tgtgtaagca
    21121 aagttataag cactgtcagt gaataataat gaaagtaaaa agacattcgt acataacgtc
    21181 gtcaatacga tagcataagt tggtgcccca tttttgttga gttttcccca tgctttaggc
    21241 aaaagtccgt cttcagccat gtttcccatt gtttcaatcg gtaagatcgt ccatgagagc
    21301 caaacaccaa ctaaagaaat gatcaaaccg ccgttgatca cgactgctcc ccatttacca
    21361 accatcattt caaacacata gcccatagca ggctgcgcct tgatcgtttg cagctgattc
    21421 atcgtcattg tcccatatgg aagcatggaa accaagacgt aaataataat caagccgaat
    21481 atccctagaa tcgttgcttt cccaacatct gattgttttt ttgctcgtga acctacgaca
    21541 gaggctcctt caataccaac aaatacccaa agcatcacca tgatgcaact caatacttga
    21601 ctggaaaacg ggatcggctg tccattcgca ttcaatataa tgtcgctaga aaagttatca
    21661 atgaattgtt ggatcgaaaa cgtgctgata gctactacaa taaataaaaa taatggcact
    21721 aatttacaga atgtgacgat agcattgacg aaactagcat tttcgactcc ccgattcact
    21781 agccatgcat atccccataa aaagactgta ctcatcacga tcgatgccag attttgccca
    21841 ccttcaaata ctgggaagaa atatcctaat gcgctcatca acaatgttgc aaaagctacg
    21901 ttgcctaacc agcatgtgag ccagtaaccc caaccgctga taaaaccagc gaaacggcca
    21961 aaaccttctt ctgcgtaact aaagatcccg ccttttaaat ctggacgttt tttattgata
    22021 ttattgatcg acaaggagag catcaacatt gctgttccta cgagtaccca actgaaaata
    22081 gctgggccgc cagccgatcc ggagatgtcc gtcataatac caaatacgcc acctccaatc
    22141 gctgaaccaa ttactaaggc agcaagtgga attaaaccaa ttcctttttt ctgttgctct
    22201 gtatccatgt aagcatcctc caaaataaaa ccgcttttat caaaaagtat aacaaacctt
    22261 ttttattttt acttaattaa taaaaacttt aatagaaata taattctacc ggcttttttc
    22321 tcattataca agcaaagcgc ctagtccctt tccaaattac accaccaaaa aacttaaaaa
    22381 agatgctata ctaaaaggaa agaattggag gatgatccat gaacatttta tatatatcga
    22441 tttcaggaaa tacgcgtgcc tttgccaaac atctagcaga atatgctgaa aaaatgcatg
    22501 cagaagatcc tgtcaatcct gaagttactt taaaagaaat ccatgaaaat agtgatttta
    22561 cgaaagaaac agaaccattt tttactttcg ttcctactta tttagacggc ggaaatggtt
    22621 tagataacgg tgatacagaa atcttgaccg aaacgatgcg tgaatatctg gaacatgaag
    22681 acaatcataa actttgtctt ggtgtagtcg gtagcggaaa taaaaacttc aataatcagt
    22741 attgtctaac agccaaacaa tatgcacaaa catttggctt tcctttcttg gcagattatg
    22801 aattacgggg aacaccatct gatgtgacac gaatttatca gattttagca gaaaacaata
    22861 aataataaaa aagaagaagc tgaaaaatag atttcagttt cttctttcgt ttctattgtt
    22921 tctatcatat taagctttga catccatcac aggtgctttt gcttttgctt tacctgtttt
    22981 ttccaagaca aagtttttta cacgatccat attggttacc aagacgatca tggatgtttc
    23041 tttgcctgct gctttgatcg cttctaagtc agcttctgct acaagcgtat caggtgtcac
    23101 ttgctgccct tctgttactt tcacatcaaa tggcgtaccg cctaagtcaa ctgtgttgat
    23161 tcccatatga agcaagattt ctaaaccatt tggcgttgtg atcccaatag cgtgtttcgt
    23221 tgggaagaca gagctgacta ccccagtaac tggagaatag atatgtccgt tttctggttc
    23281 tacaaagtag ccttcaccca tcatcttttg tgcaaaaact ggatcacttg ctttatctaa
    23341 agaaacaact gttccgtcag caactgatga gaggatagtc acactttcag ctgaagtttg
    23401 aggtactact ttttcaggca cttcatttac tgtggctgtt tcagtcgcta attcttcttt
    23461 ttctaaacgg tctttaggaa taccgaagaa gaatgtagca acgaagccac cagcataagc
    23521 tgccaataat ccaaggatgt agcctaacca tttaccgtca gagatcaatg ggatcaaagc
    23581 ggctcctgaa ggtccaatag cgatagcccc gacatttccg atcattccga tcactgcccc
    23641 gccgatacct ccaccgatac aagcagtaat aaatgggcgt cctagaggta atgttactcc
    23701 gtaaatcaat ggttcgccga ttcctaggat cccgactggt aaagctcctt tgatcatttc
    23761 tgttaattct ttatcttttt tgcagcgaat ccataatgcc aatgctgcac ccacttgtcc
    23821 tgcaccagcc atcgcaagaa ttggaagcaa tagcgtacga ccagtttcat caatcatttg
    23881 cacatggatt ggagtcaaaa tttgatgcag tccgaacata accatcggca agaataatgt
    23941 gcctagaacg aagccggcaa atgctccacc aacgccaagt acccagttga tcccaccgac
    24001 catgccatca gaaatgaaac ctgctaaagg catgatgaag aaaatttcaa tcagaccgat
    24061 ggcaatcaat gaaagagttg gtgtaacgat gatatctacc gcatcaggta ctactttatg
    24121 cagttttttc tcaacgattg acaatagcca aacagcgaag atgacaccta ggatcccacc
    24181 ttgtccagct gcaagagctt caccagtaaa taagtttttc agtggtgctt ccggattcat
    24241 ccctgtcagc atcacaacgg ccccgataac tccaccaaga gttggtgttg caccaaaaac
    24301 ttgtgcagca ttgatacctg tatagataac taggtagcta aacatcccgt ttttcaagat
    24361 gttcatcaca tcgatatatt gttgccatgt tgctgcatca agtgttccgg ctgtgactaa
    24421 gtttgctaaa actgcggcaa tccctgccac gatcccagta ccgacaaatg caggaatcat
    24481 tggcacaaag atattagaaa tgtcttttaa taccgatttg attttagatg gtttttgttt
    24541 cgatttttgt gccgctttca tttcttgtgc ccgttcgttt acacggtctt ttccagatgc
    24601 tgatgaattt gctgtagctg tacttacaga agaaagtttt tctcctaatt tcacgcctgc
    24661 caaatcaacc atttgctgtg ctactttgtt cactttccca ggtccgatga tgacttgaag
    24721 ctgttcatct tctactacac caagcactcc tggaatcgct tttaattcag agacatttac
    24781 aagatcatca ttgatgatct tcatacgcac acgagtcatg cagtggacaa tgctttctac
    24841 attgcccatg ccgcctgccc ctttataaat ttgttttgcc agttcttcca aagtcagttc
    24901 gttttttgcc atggtctttt ccccctcata aggtcccttt tacaaatccg tttgcttcag
    24961 ctaattttgc ttgagcttcc tctttatcac tatttgtcaa aatcatgacg atagccagct
    25021 taacattttg atctgcttct tcaaagtact tttcagctgt ttcgtaatca gcttctgtcg
    25081 cttgcatgat gattcgtttt gagcgttcga ttagtttttc atttgttggt ttgacatcga
    25141 ccatcaagtt attgaatact ttacctatac caatcatact gatagtagaa atcatattca
    25201 agataagttt ttgtgctgtt cctgacttca atcgagtaga gcctgtcaaa aattctggac
    25261 ctgcatcgac ttcgattggg aaatccgcgt gtttactgat ttcggcttgt ttattgcaag
    25321 aaatcgttcc tgtgacagca ccgatcgatt ttgcgtaatc taatccaccg atcacatatg
    25381 gtgtacgacc gctagctgcg ataccaataa ctatatcatt ttttgtcaaa ttcagcttga
    25441 ttagatcttc ttgccctagc tcataagaat cttcggcccc ttcgacagct acagtcattg
    25501 cttcttctcc accagcaatt agacctatga ccatttccgg ttctacaccg aatgtcggta
    25561 cgcattcagc agcatctaac acacctagtc ttccacttgt tccggctccc atataaatca
    25621 gacgtccacc ttttttaaag gactcaatcg tgttagcaat cactggttcg atacttgtca
    25681 gttctttttc aaccgcagcg gccactttct gatcttcttt gttcattagt tgaacggctt
    25741 gagaaatact catctcatct aatccaaaag tttcttggtt tctgcgttcc gttgttaggt
    25801 tctctaaatt gattttgctc atttttatct tccttctttt cttataaatt gatagttcat
    25861 accagctttg atttggttga tcaatggtag ctcttctttg ataactttag ctactacatt
    25921 cactttttca tccgctggca aatcatattt ggttaattgg atctcaccca tatagcgcaa
    25981 atacttttcg ttatctaaag taatagaccc tttcatacgc gtagctgtat ttcttgctgg
    26041 gataggcgga atctctcgaa aacgtgcatc tacgcttcgt atgacatctc ttgcatcgtc
    26101 ttgccgattc gtatgcttcc ctaatatata ttcgtaaaat tcttcatcta caggttccgt
    26161 atggagcaag actgtctgtt ctttttggaa agaagcaaat tgctcctgaa cgttttctga
    26221 cagacctgca tctccgatgt aaacaatatc cgtgttactt gctaatagat ctagagcgcc
    26281 tgcaagagga tggatccctc gatgttcttc caaagttggc aatccttgat atagtgggcc
    26341 tctcaactct gtatctcctg gtacaaaacc ttggacaagg agaccttgaa ttttgagcca
    26401 ttgatttttt ttctgatacc attctttgtc cagccctgtt tcgggacgtg gataataatt
    26461 atgccaagct tcaatctgtg agaaatcagc ttctgcttct ttcaattcat cgatatcttt
    26521 aggcgtgatc gtgctagcat tcaaactgat tttcaattga tgtgaccagt cggcaatctg
    26581 ctgattggaa atatgataat ccattcttaa acctgttaca ccaattgctt tcaaaggcgt
    26641 tagatcttct aatgaaaaac ccgctcgttc caatgcctct cctgaaatat cgaccatcaa
    26701 ttctagttta ttctttttcg cttcgcttcc tagttcgatc aatcgctttt gatatgccga
    26761 aacatcatct tctggtatat gcatggaagt gaaaataccg acaaagccat tggatgccat
    26821 ttggcggata tactgtttct tctcttctga caaagaatcg ttcataaata ttgaaaagcc
    26881 aaacatatta tccctctttt tctgtatccg tcttcatttc tatgactaga gtatatatct
    26941 aaagaaacaa aatttcaata cttttattaa aataattaaa tttcatttct aaaggttgag
    27001 tttatattat caaaagaatt gtcataacct aaaaaaggca ctgctaagca aaagcagtgc
    27061 cttgaaatat tttatttaaa taacacaaaa acataattac tcataagaaa ccaattctgt
    27121 attatctacc atgaatgtaa agaacccctc attaggcatc ggatgaaaat aagcattgat
    27181 cactcttatc aaggcattat ggcaaactaa cagatagaca ttttcttcgt cttcgatgca
    27241 ttctttcaat aaaggaacga tccgtgcata cacatctaag actgactccc catttgggaa
    27301 tcgtacagcg aacgctaaac gtgctttttg aaagttctca tctttgcttg gcagtccatc
    27361 ataatcccca aaatccattt ctaccaatcg ttcatcaacg gtcagcggca agctgagacg
    27421 atcagcaact gcttgtgctg tatctcttgc tcgttgaaga ggggaatgga tgattttcgt
    27481 gattggaaca ggcaaatcta ctaccttttc tgctagtttc tgtgcctgca tctgtccttt
    27541 ttcagtcagc ggcacatctg cccgtccgca aactaatcct tgtgcgttcc aagttgtttc
    27601 tccgtgtctc gttacataaa gcatgtgatt ctctccttcg ttggcaaagt tccccttaac
    27661 cataccttgt tgttcttgag attacaaggg ttagattctc tcaagagaaa taatctcagt
    27721 agatacttta tccaggaagg tttgatcatg ttccactagt aacatggcag gtttcacgtt
    27781 caagatcagt tgttctaatt gttcttgatt gaagacatcc aaataattca atggttcatc
    27841 ccatgtatat agttcagctg gctgtgataa agatttagcc aattccactt ttttccgttg
    27901 gcccatactc atctgctcga tcttgttatg aaaaacatct ctttccatcc caagctttcg
    27961 gaggttgttc aaaaatgctt ggtagtctac ttggtttttc tctgcaaatt ccgccaacgt
    28021 tcctcgattg tcttcataat tttggcttgc ataactaatg ctcagatgtt ttggctggta
    28081 tttttctcct ataaccttgc cgttgaatgc ccccagaaga taatggataa tggatgactt
    28141 tcctgcaccg ttaggacctg aaatcgccac ccgctgatga ggctcgattg taaaatgaat
    28201 tggctcgaat aacagatttt cataccctaa ttgaagatct tctacgctta aaagtcgctt
    28261 atggtgagac gcttggctat tcatcgtcaa agaatcgata tattcgatat cttttaatag
    28321 tttttctttc tcgctgatct gggtctccat ccgattcacg attgctttgg atcgtttcat
    28381 cgtccgtgca gcatcagcac ccactgctcc tttattcact cgtctagatt cagtatcgat
    28441 gaatccgact tgtttctttg ttttatctcc ttctcgggaa cgagaccatt cggctttttc
    28501 agctgctgtt ttctttagcc tactgacttc ttttttcaat ttttcatttt gagccatttc
    28561 aaattcatca cgaagttttt tctgttcttc atagatagag aaattccctt gataaagttc
    28621 cagttgactt ttttcgattg ctaaaacatg gtccactact tcatcgataa atccccggtc
    28681 atggctgacc acgatgaagc cttgtttttt ctttttcaaa taagccgcta cttgttttct
    28741 accagagata tccaaatgat tcgttggttc atcgattaac gggaaatgag tgtcatccac
    28801 aaataaaagt gccagtagga cttttgtctt ctctcccccc gatagtgtgc tgaattctct
    28861 ccataagatt tcaggatctg tttgcatcaa ttggagctct ctttcgattt cccatatctc
    28921 aaaatccgta atatcattta acacgtaata ggttaaacgt tctttatctt ttgtctgttg
    28981 cgggaaatag gcaaattctt gctgatggat tacttgccct tgataaggta gtttgttttg
    29041 tagaatattc agtaaagttg tctttcctcg accgtttcgt ccgataagtc ctagtttcca
    29101 ttgtgtgtca aaatttagat ttgcttgttc aaataataat gtgccttggc tgtcgtagcc
    29161 gaatgtcaga ttttttattt cgattttaga cattttccat cacctcgtaa aaaaagacca
    29221 gtcctatttt cagaactggc taaagaggat gcaagggttg gatacacaca aaaagaccca
    29281 tacatcgaaa aaaccaagcc aatcccgaaa atctgcacac tgcttccttg tctccacaag
    29341 gtacagtctg aatgcagctt tcatcaatcg acttaatttt tcaatgcacg gatccctcac
    29401 tttgtcctat atttgaaatt acgatagcat aaagaaaatg attttgtcaa ctacttcggg
    29461 aggatggtga aaaatctgct aagtgtctgt cctaagattt ttgttttttc taatgtttgc
    29521 ctgtgttcca ttgcttcttc taaacctatt ggacccaaat gaatacttaa gacaattggg
    29581 agaaaatcag aaagtccttc tagctcttgg tctctacttc cgcaaatagc gattattggt
    29641 tttcgatatt tttttgcaag cactgctaca ccgtatggaa cttttccgtg gatcgtttgc
    29701 tgatcgatcc gcccttctcc tgtaatgatg acatctgctt gttcgatctt tttttccaat
    29761 ccagtgattt ggctgactaa atcgaaacca gaaaccatct tcgctcccaa caaaaaaagg
    29821 gctccaccaa taccgcctgc tgctccactt cctggaatat catctaaatc cacctcccag
    29881 aaatctttta tttgctgagc aatctttcct gcttgtttat ctaagaaagc tagcgtcgta
    29941 tctattcctc ctttttgtct tccaaattgc caagcagctc cttgttgacc tgtatatgga
    30001 ttggtaacat ctgcagcgat aatcaatttt actttagaaa caagaacgtt tcttcgatca
    30061 aaatgaagtt ttttagtaga taataaagga tttccttctg tgtacccttc taaagaagca
    30121 cccaaggatt ggagtaatcc tagaccacca tcgctacacc cgcttcctcc taaagtgaca
    30181 ataatttgtt ctacttcttg aagcagtgca tctttaataa gttctcctaa ggcataactg
    30241 gaagcaagtc ggatagtttg atccgttggt ttatcaagta gatctaaccc caatgcttgt
    30301 gcagattcga tgactgcggt gttttttcct tgccaagttg tcaataaata gctgattttt
    30361 cgctgacgga ataagagatc catcccatca atggtacgcc atgttcccct gagcgcgtaa
    30421 tagattgctt ctgcagtatt ttctccgcca tctgcaatcg gcacattgat ttttcttcct
    30481 ttacgatctt cccagccatt tagaactgcc tgatttagtt caaatgaaga agcacttcct
    30541 ttaaatgaat caattgccgc caaaatatcc atctgtatcc ctcactttct tcttaagtaa
    30601 gacacttttt ttcatttttt tcaaacaaaa caatgctatt ttcttccatt tttctatata
    30661 ctaaaaggga actcattgaa taaaggagag aaaaaacatg aaaatcgcaa tagctggggc
    30721 cggagcaatg ggcagccgaa tcggattgat gctacatcaa agcggaaatg aggtcctctt
    30781 gatcgatcgt tggcctgctc acattgaagc cattcgtaca aatgggttga ttgctgactt
    30841 taacggtaaa gaagtggtcg caaaactgcc aatctattca ccagaagaaa tcatcgaaag
    30901 caacgaacac gtggacttga tcgtagcatt aacaaaagcc aatcaattag atgatatgtt
    30961 ttgttcgatc caatccatta ttactgacaa tacttatgtt ttgtgtttac taaatggatt
    31021 aggtcatgaa gatgtactgg aaaaatatgt gccaaagaaa aatatcttat ttgggatcac
    31081 gatgtggaca gctggtctag ctggtcccgg aaaagtcaca ttgttaggtg atggagaaat
    31141 cgaactagag aacttggaac cagaaggcga agcatttaca aaaaaagttg tagaagtctt
    31201 tcaagaagca aatttaaatc cgatctatag ccataacgtt cgttattcca tttggcgaaa
    31261 agcttgtgta aatggaacat tgaatggttt atgcacgatt ttggattgta atattgctga
    31321 actaggcgcg caaaaagcgg cagagagcat ggttcgaaca atcgtatctg aatttgcttc
    31381 tatcgcagca aaagaaggaa tcatactaga tcaagaagaa gtgtaccaac atattgcttc
    31441 tacttatgat cctgacaaca tcgggttgca ttacccatct atgtaccaag acctgatcaa
    31501 aaaccatcgt ttgactgaaa tcgactacat caatggtgcc atttggcgaa aaggacaaaa
    31561 atatgatatt gctacaccat attgtgcctt tttgacccaa ttagtccatg caaaagaagg
    31621 aatccttggc gcagaataaa aggctctctg atgagaatca gctgaatcag cagctggttc
    31681 tcttttttta agcccaataa aagatcaaaa agaaaacaca actctcatga tgccataaga
    31741 attgtgcttg tttgtttcag tacataatct cttcaaaatg taggtttttt atttgttttc
    31801 aacagaggag aggtcatatt cgtattgctc ataaatagtg gaatcgttag tgataaatcg
    31861 ttttgcccaa ttttctgcat cataaaattc caatagctct gataggattg gcttaaatgg
    31921 tgtatgcaga aattccggta attcatctaa atcacgttct cgaagcaaaa aaatattttc
    31981 aggacgataa aaatgataat gagcaatcgc ttgattatcc gtcactagtt tcttttgaaa
    32041 atacatggat tcaaatgtcc gcaaagtcac tccttgctga ccctcccgca acagttccag
    32101 aatcccgcgc gattcccctg tttttttcag gtactcttca taagtaagat atccaggttg
    32161 ttcattccct cgatgtggaa gaatgaaata gttggtcgta acatctcttt ttataaattc
    32221 ctgcttatag ctttctgctc gttcttttct cccattattc gttgcaccaa aaaatagatc
    32281 cgtctttggt tcgctagaag aaaaattcat atgtttcgaa taaaatgaac tattatgttt
    32341 cagcccaagt tcttttgctt ccaaaaaatc aaaatgataa atctcatcga ttgccggttc
    32401 atcttgtgcc tgcttcagca tcgcctcgtg ttctgctttg aagtgattcc aaaaaaataa
    32461 cacaattttt gcattggtaa atgggcgcac gcgtcgagcc atctccatat caaatccata
    32521 atctaaaaaa acgatcagct gataatttgc caactgttgt ctagctgatt taatgaattc
    32581 cgactgtagt aaatcttttc cggaccaaac atctaaagag cggatggaag gcaaaaaata
    32641 aagtgattta ttttttgcaa ctagcagtac gtcatttttg ttcatctttt ccgcctcact
    32701 ttctctgtca ctttttccta tgatagcctg tttaaatagt aaaaagccag taaaacgttt
    32761 cctatatttg tatgaaaatt taacatttgt atgaaaatat gatgaaataa atcattcttc
    32821 tattaaaatt cgcctaaaag ttttctgaat agttgtaatt gtctgaaaat ttgttatcat
    32881 tagtcattgt taaatattgc tatttattct taatataaat tttgattgtg aatttaaaag
    32941 aaagaggtca tttatggaag ataaaaaatt gaaaaaacaa cttagctcca gacatatcac
    33001 catgttagct ttaggtggag caatcggtgc aggtctattt aaaggaagtg gcgaagcgat
    33061 tgggatcgcc ggcccttctg tattgattgc ttttttgatt ggtgggctga ttttatatat
    33121 cgtgatgaaa ggtctgggaa aaatcgtttt aagcggtggc gacacgcatc acggattatc
    33181 tggtttgata cgcccttatc taggtgctca ttctgctgat tttactgatt gggtctactg
    33241 gtctatgtgg attatcaata tcatcgctga agctgttgca gctgcttcct ttttgcagtt
    33301 gtggttcccg cacattccag cctgggtatt tgtttttatg ttggctgttt taactacgat
    33361 catcaatctt tattcggtac ggttattcgc agaaacagaa tattggctgg cttttgctaa
    33421 aatcagtgtc attatcttac tgatcatttt tgctacttat cttgtcggtc agcaaatgtt
    33481 gggcacaggc gttttcccta cccttcagca attgactgac catggtgggt ttactcctca
    33541 tggtatgaag ggaatcatca gttctttgct tgtagttatc tattcttacg gcggttctga
    33601 actgattgct atcacggtaa gcgaagcaga tgatcctaaa cgtgcgattc ctaaagcaat
    33661 caaaggtgtc atgggaagaa tcatttcctt ttacatcatt cctttgtttt tgcttttgat
    33721 tctttttcct tggaatactt tagcaggcac gaatgtcagt ccttttgtga tggtatttga
    33781 aaaaatgaat atcccatttg cagcggatat cgtcaatttt gtgattgtcc ttgctttgtt
    33841 ctcatctatc aattcgggtg tgtatgcatc ttcaagaatt ttgttcttcc gtttgaagga
    33901 tcgaaaaggt tcctctagaa aactagctgt tttaaacaag catcaagtac cgcaacgtgc
    33961 tgttttattt tgtaccagtg ttctttatct tggtgtagct ctttcttatt ttgtcggtga
    34021 caaattattt ggctatctgg caggttccct ttcgtataca gtattattga tctggatcat
    34081 tatcagtgcg gcatcctttg tactggcact aagaaaagga acattatggg ataaaggaat
    34141 cagctttttt gcattagctg tcttaggatt gattttcctt ggtatcctgt tcacgaactc
    34201 cattggggta actgtattga ctggcctgct ttacctgttc atttatttca gttatcaaaa
    34261 aaagaatgat gcgttcgtat tgacgaatga atgaaatcga tagaaaattt tattttattt
    34321 atgtggaata gatggagcta ttgaagaaag gagttgtgac aaaagtcttg ttgcaactcc
    34381 tttttccgaa taaacagtct catgacctct tggtaaacgg tgttcaaaaa ccctctgtgc
    34441 aaaataattg attgttctcc atgctcatga catgatagaa gaaaggaatg aaaaaaagga
    34501 gtttgtttta aatgaaaaag tattggaagt ttttgttgat gatcggtgtt tcaacggtag
    34561 ttatgtttgg tatgatgtac ttgaatgtct atgcgctcga tcatatcttc ttcagtcgta
    34621 cacgagtatt catggcgttg atgatgggtg cagtcatggc agtcatcatg ctgttgttca
    34681 tgtggaaaat gtatgaaaac aaaaagataa atgttatgat cttagccgct agtcttcttg
    34741 tttttgccgg ttccttgttt atggtacgaa gccaaactac tgtcaacgac acagcttgga
    34801 tgaaggccat gattcctcat cattctatcg ctatcctggc aagtaaacga gctgatttaa
    34861 aagatcctga agtaaaaaaa ttagcggaag aaatcattca ggcacaagaa gaagagatcg
    34921 acaaaatgaa aaaaatgatc aaagaaatgg aatgaccatt atccgtccaa ataaaaaaag
    34981 aggatgttcc agaagttatc agcttcaaga aataagaaga agctacttct ggaacgccgt
    35041 gaataagggg ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aaacaaaaat
    35101 agcttctcct atttttcgtt tggttaggct tattaccgca gatgcaagct tttgaacacc
    35161 gtttattaag aggttgtaaa atcagcgttc tgacctgagt attagagaga caaatggaaa
    35221 aatagttcct catattttgc cattttttga gctaataccg agggtcagct gattgaacac
    35281 cgtttatcga gaggttgtga aaaagtcgct tagtttcgcg tattaagaaa aattttgtga
    35341 aatggctttc cacatttttg tataaatttg gcttaatacc gagaaactgg cttttgaata
    35401 ccgtttattc gtaaaaggga ttgtgacaaa agtcttgtca caatcccttt ttatgttttt
    35461 attgttctaa ttgcgctgtc ttgcttactg cttcttgcgg tttcacaaat ttttttgaga
    35521 ttccccaacc tgtgaaggca ctgaccatcg aaaacacagg agaaagcaaa ctgaaaaata
    35581 cgaatggcaa gtaagtgagt gtaggaatac caaatgtact tgctacgaaa cttccggcaa
    35641 ttccccaagg cactaagtag ttgattactg ttccaccatc ttctagcgtt ctacctaata
    35701 cagttggatc aaatcctgct ttttcataag tttctttaaa tgcattacca ggcagaataa
    35761 cagataggaa ttgttcacca ataaaaatat ttactccgat acttgtcatc aatgttgtaa
    35821 aaatcaaatt tggtgtgttc gtcatctttc ttgatactcg ttccatcacg gttgagatca
    35881 agccaaattc gatcaacaat ccacctaatg acaaagttag aataatcaat gacacagtag
    35941 gcatcatact ttcgattcct ccgcggctaa gcagtgaatc gatttgttgg tttccagtat
    36001 ttgaaacaaa ccctgattcg atgaccccag ccaacttcgt taaggaagtt tgtgggtttt
    36061 gaataaaaat cattcctact gctacagcga tattcaacaa aatcgttaaa attgccggca
    36121 cctttttcca tgcacacagt aacattaaaa ataaaggaat cagtgaccag attgaaatag
    36181 taaagtttgc ttctagaaca tcagtcactt cacgaataga tgaaagactt gcggctttac
    36241 ttgtgtgtcc taaaatcatg aataaaaaga aagaaacaac aaatgccggg acagttgacc
    36301 acatcatatg cttgatgtgt ttgaacaaat cggcatcaac aatagctgct gctagatttg
    36361 ttgattcgga taatggagac atcttatctc caaaaacggc acctgaaaca atagctccaa
    36421 caaccagcgc tggatttaag cctagtgttg tcccaatacc gaaaaatgca atcccaatcg
    36481 ttgacataac agtaaaggca ctccctacag cgctacctac gattgcacac acaacaaata
    36541 cagaagggac aaaccactgg acactgatca atttgaaccc aatgaccatt agagttggaa
    36601 tgatccctgc ttgtatccat acagcaatca atgaaccgac taaaataaaa ataaagattg
    36661 ggatgattcc tggtttgatg ccattgatga ttccttcgtg cagccaatct gtcgggtatc
    36721 tttttatcac tgcatacaac atgaccattc cgatcgctgc taagatcgtg atattaggtg
    36781 agacctttaa tccgacaacg ccaatcgcaa tgcctaaaac aataaccatt aatacaagtg
    36841 ttgcttctaa aaaacttggt gttccttttt ttcccatgat aatctctcct tttttagttg
    36901 ttcttatgtt ctaaaaaagt taagatatcg gattactccg ccgcaattca tccgcgattc
    36961 ctcctttata aaaataaaaa gacctgtttt aaactaagga cgaatgactc cgcggtacca
    37021 ccttaattaa aaacagatct gtttttcact ttcaaacttt tattcaaatg tatggacttt
    37081 ccatgtaatt cgtcaattag gcttgatcgg ttcacagcga ccaccgactt tctgaacaaa
    37141 acacctaacc gctactgcgt gaaaagttga cttgtacgaa gtttaccttc cataagatca
    37201 tttgtcaatg aaaaagaaaa tattttttca tttttctgaa aattattttt ataactttca
    37261 cacacaagtt tattttttac tagaaagtag cgccctcttg tttttttctt gctactattt
    37321 cttgatgttc ttgcttgatt ctttctaata gctttggctc tttcaacaga tcaagagctg
    37381 tattggcgag tagttctgct gcaatcgcaa tagaagcaag gcctttgtca cttttagcag
    37441 cagctttgaa ttcttctgtg tgtccggcaa tatatgtctc agagattgct accgtaggct
    37501 ggattgttgg tatgacttgg ctgacatttc ctacatcaga agaaccgaga cttgttcttt
    37561 gttccgtttc tatttcttca tctggaacac ctgcttcttt tgcatgctgg aagaataagt
    37621 catcaaaaga aggagtaacg atgacatcat caactgcatt ttggaacaat ccaaactcaa
    37681 aagtcgtgcc cgtacttatc gcagcacctc gtacgatatt ttccactttt tcatatactt
    37741 tatctaatgt ttgccgattt cctgcacgta aatagaaacg tccagcagcg taatctggaa
    37801 tgacgttggc agctactcct ccgtctgtaa tgatcccatg gatattgaca tcttttggca
    37861 attgcaagcg tagagcgttg attccattaa acacttggat caatgcttcc aaagcattta
    37921 ctcctttttc aggtgctgct gctgcatggg aagcgacacc gtaaaatttg atgtcgactg
    37981 gatcattcgc taaagattct gtcgttttcc cataacgaaa agctggatgg acgcataacg
    38041 ctacatcgac atcctcaaaa aaaccttctc gaacaaagct gcctttagct gatccgttct
    38101 cgcccccttc ttcccctggg gtgccatata cacggatttc tccaccagtt tcatcaatga
    38161 attgcttcac tatgctggcg gctaatacgg agtaagtacc aaacaaatta tgcccgcatg
    38221 catgaccaat acctggtaaa gcatcgaatt ctgccaaaaa ggcaattgtg ggaccgggtt
    38281 tattggaaat ataacgtgca tcaaaggctg ttcggtgtcc agctacttct ttttctaccc
    38341 aaaacctttc tttttctaat tgtttaatca acgctgctga ggaataaaac tcatagttac
    38401 ttacttcagg atgttcgtgg atatctagag caagtgcttg atattctggt aaacgatatt
    38461 ggattagatc cttcaattgg tttgttgaga ttgttgttgt cataaaaaat tcctcctatg
    38521 ctagtcattt ttcttatttt ctcccgaatg tttcccatgc aggcacgctt gatccttttg
    38581 atgttttctc gattactttg actgtgtctt ctgtttgata agcgtctact attttttgat
    38641 acgttttatt gttttcatct tcttttcttg caacgatgat attcacataa ggacgtgcat
    38701 ggtcatccac tggttctaag aagatggcat cagaagcagg attaaatcca gcatctacag
    38761 ctacaccgct attgatcact gaaatgtcta catctgacaa agcacgtgct gtttgggaag
    38821 catctaattc agaaatgtct agattttttc tattctcggt aatatcagaa acagtgggcg
    38881 cttgtccttt tgccttatcc actttgatca atccagcagt ttgcaataac agaagagctc
    38941 tgccgccatt tgtaacatca ttagggattg ctacttttgc tccgtctttg atatcagcta
    39001 catccttcaa agtgttagaa taaattccca gcggtgcatt gacagtattt ccgatcgata
    39061 ctaaatcagt tccgtgttct ttattgtaat tgtctaaaaa gaattgatgt tggaaagaat
    39121 tcaaatcaat cgatccatct gctaaagcca catttggttg attgtaatct gtaaatttca
    39181 cgtattccaa attaatgttc tcctttttca aacggtcttt cacgttgtcc catacatctg
    39241 tatcttctcc aataatccct agttttacga tagtttcttt tttgtcagtt gtatctgctt
    39301 ttccttggct acaccctgcc aaccaaataa ttgctacaaa tgtaatgact acaaaacgaa
    39361 taattttttt cattttttct cctccacttt caatgtgtaa tttttttaat cagttgatta
    39421 ctgataaatt gacttaaaaa aactaagatt agaatcaata atgtagccac aatcgttaca
    39481 tctgtttgga atcgattata ccctctcgat atagcaagat tccctaatcc gcctgcacct
    39541 actgctccag ccatggtagt cagtccgatc acgttgatga tagtcaacgc cgagacccga
    39601 atgataccag gcaaaccttc taacaaatac acacgtaaaa tgataccgat tggactggtt
    39661 cccatcgctt ctgccgcttc gatcacccca ggatcaactt ctagcagtgc gttctcgatt
    39721 tgacgagcaa aaaacggaac aactccaaaa atcaatggca ctaaagctgc tttcgcacca
    39781 atcgtcgttc caactaagaa acgagtagtc aatgctaaaa gagaaagcaa gataatgaaa
    39841 gggatggaac gaatgatatt gatcagctta tcaagtattt caaatagcag acggttagct
    39901 agtaatccat tagggcgtgt cactactaac gtcacaccta agatgattcc aatcatcccg
    39961 ccaatgaatg ctgtccaaaa agacatatat agtgtttgct tcgtcgcttc gacaaattca
    40021 tccggaatca aataaacatt gggcaaatat gttttaaaga aatcagtcat tttatcttcc
    40081 tccttcgatc actttgatac gactatctaa atcgtttttt tgtaaaatat ttatttttac
    40141 accagatttg atcaaataat ccaatgcttt tttccgctgt ttggtttctc cagctaaagt
    40201 aacgatcaaa ctccctaacg gcgtttcttg taataactct acattgccat ataaaatatt
    40261 cgctgttact tgatatttgc tgtacagatg agcgattaga ggttcgttcg tctgatttcc
    40321 aatataactt aattccacca gccatttatt ggcgatctga tcggcaaaag cagaatgggc
    40381 aatgatcgtt tctaatgctt ggtcaagatg agtagctgtt cgaataaaat ctttggtcaa
    40441 ttcttcttca ggattgctga agatctggat gctgctacct ttttcaacga ttcttccatc
    40501 ttccataacc gctaccttgt tgcagatttc tttgacaact tgcatctcat gagtgatcag
    40561 gacgattgtt aaacctaact ggcgattcaa tttttttaac aatgcaagga tctgaagggt
    40621 cgttttcgga tcaagtgcac tggttgcttc atcacataat aataccttag gttcattcgc
    40681 caaagctcgt gcgattgcta ctcgttgttt ttgtcccccc gacagttgtt taggataagc
    40741 agatgctttt tcttccaaac cgacaagttc cagtagttca ttcacttttt gacgacgttc
    40801 ctgtttcgat tttccagaat actttaaaga aaaatcaacg ttgtcaaaaa tagttcggct
    40861 ttccattaga ttgaaatgct gaaagatcat accgatggcc tttctttctt ttcgcaattc
    40921 tttgggagag agctgactca gattcgtctg attgacaatg acttctcctg ctgttggttt
    40981 ctgcagcaaa ttcatcacac gaactaacgt acttttccct gcacctgagt aaccgacgat
    41041 tccataaaca tcgccttttt caatcgttaa atctacagaa tctactgcct gtatctcttt
    41101 ttctttttgt ttgaaacata cagaaacatc tcgcaattca atcattaaaa ttcctcctta
    41161 tctaaaaaaa gcgtcctcaa gaaaatgaaa atgtcatttt cttgaggacg cttttttgcg
    41221 tgttaccacc tcatgttcaa gctgtgttca cacacaactc ctcttcaagt acgatcatac
    41281 tctagcaaga tatcggtcgc agccgtcatc gccttacatc tatgctcggg atgaattgct
    41341 cgatggccat tttccaatag tcgttccttg cttcttctca gctaccgaag ctctctgtca
    41401 aaggttcttc tatcgtactt accatttcaa tgcaaataat attcaaaaaa aaagtccttt
    41461 tactaacata aactatgtca gtaaaaggac gacgtatcgc ggtaccacct tttttcggaa
    41521 gtttccttcc ctcaagccca acatctcaaa aagagagatt ggcgttcgtt ttaacggtcg
    41581 aacagttccg aatttgccta cttatcgaca aattattctc caaggccatt ttcagctttt
    41641 cttacttgac tcttctcacc agccagagcc tctctgcaaa gtttccaaac ttactttcct
    41701 tttcaacgaa atcgatattt tgttgttggg attaatctac atgaggaaaa ctgaaaagtc
    41761 aagtacgaaa ttcaaattat tttttctata tttatttttc tgttttttta tttaacattt
    41821 caataacagt tcgaagatct tttccttccg caagaagttt caatttttcc ttttcttcga
    41881 cggtataatt tcggacaaaa ctggaatcag gtatcaaaac aacgcctaat cctgctcgtg
    41941 aagcagctgt tgctcctact aaagaatctt ccactgcaaa ggctgaacta gcttctaatc
    42001 ctgttcgttc caaagctgtc agataaggaa tcggtgaagg cttgttctcc gacacttcgt
    42061 ctccgaatgt aaacgtgtcc aagtaatcag ctaatttaaa gtgatccaag atatctgttg
    42121 ctcttttcgt aacagtggtc gtagcaatgc ctagtttcat atgacgttct tttaagtctg
    42181 ccaatatttc ttttgcatat ggttttaagt ggatggctcc gcttgtcagc tgttgataaa
    42241 aatattcttc acgcttcgct cggacttcct gtactttttc agggctacct gctgctttca
    42301 ctaaaatatc aaaactttgt ttccagtttt ttccgcccca agcgtcaacg atttcttttg
    42361 ggatatggat atcaaattgc tgaaatcccc aaagccagcc atcacgatac gttttttctg
    42421 tgtcaactaa caaaccatct aaatcaaata ttgcaccttt catctataca ttaatccttt
    42481 ccaaaacaat acacatagtt tagcacgact ggtccaaaaa gaacagaagc aaagaaccag
    42541 ccttctcgtt tataagagtt cttggaatcg acggatatag attttcttca cagctgtcac
    42601 aagcgccaga taagcgatga ctgtagcaaa taaccataac cagtaattgc caggaagtgg
    42661 catcaagcct aattcagcac caaaacttgt aaaaggaagg acagaaccga caataattcc
    42721 taatgtagtg ataacaaaca tggcaaatgt tgcgttgctt tggataaatg gtactttcgg
    42781 tgtacgcagt gcatggagca ctaaggtttg tgtccatagt gattcgacaa accatcctgc
    42841 atggaataat gcgatgaaaa tcactcgctg tgaactatcc aatgtatgga aacttccgcc
    42901 cactacagca ggacaaataa caaaatacat caacaagtaa gtagtaatat caaatatcga
    42961 acttgttgga ccaaagtaaa ccatgaattc cccaatacta gatgcattcc atttttttgg
    43021 ctcgtccaaa tattctttat ccatccgatc ccatggcaag gaaatgcagg aaacatcata
    43081 gatcagattc aaaaacaaaa gttgtaaagg cagcattggt aaaaatggca ggaacgtact
    43141 tgcaatcagc acggaaaaca tattgccaaa attagaactt gctgtagcct tgatatattt
    43201 catgatattc ccaaaagttt ctcgtccaga aagaaggcct ctttccaaaa tcgtcaaatc
    43261 tttttctaaa aggatcacgt ctgcagattc tttcgcaata tccacagctg tatctacaga
    43321 aataccgata tcagccactt tcattgcagg agcgtcattg ataccatctc ctaaaaatcc
    43381 gacagtatga ccatttttac gtaaagctgt cgtcaaacgt gctttttgct gaggattcaa
    43441 ttttacgaat acttcatggt cttcagctac ttgtgctaat tcatgatcac tcatttccga
    43501 gattttctca ccggtaacca attcattgat ctctagacct acttgacggc acactgaacg
    43561 tgtaactaat tcgttgtccc cagtcagaac ttttacagct acaccatgtt ctttcagagc
    43621 tttcaacgct tgtttcgttg tttcttttgg tggatcaagg aaagcaagat aaccaatcaa
    43681 aaccatgtct gactcatctt tgactgaaaa ttctccaacg acgcttggat tggtcttttg
    43741 tgcgacacca atcactcgta agccatcttc attaagtgac tgtacttttg caagtacggc
    43801 ttcttttcgt tcttttgtta atggtgtaac agtaccgcca agatcaataa atttagaaat
    43861 gttcagcatt tcttcaatcg cgccttttgt gatcatttgt gttttaccag ctggatcagc
    43921 aaccacaaca ctcatacgac gacgttcaaa atcaaaagga atttcatcca ctttttgata
    43981 attgatctta tctgtatcta aagtttctgt tgcttcatca atgatagcca catctaacaa
    44041 atttttcagc cctgtttgat agtagctatt tagatacgca tggcgcaaaa cacgatcatc
    44101 ttcatttcct tcacaatcca tatgatattc aagaatgatt ttatcttgtg tcaatgtccc
    44161 cgttttatcc gtacataaca catcgattgc accaaagttc tggatggcat tcaaattttt
    44221 gatcactgtt ccttttttgg ccatcacact tgctcctttg accaaattcg tcgtaacgat
    44281 cattggaagc atttcaggtg ttagaccaac tgctacagat agtccaaaaa gaaaagcttc
    44341 tagccaatcc ccttttgtaa gcccattgat gacgataact gctggagcca tcaatgacat
    44401 aaatttgatc agtagcatgg atgttttatg gatgcctaat tcaaagcttg attgaattgg
    44461 tttttcagat agcgtttctg ctacatgacc aaatagtgtt tcatttccaa cagaaacaat
    44521 gattccttcc gcacttccac tgatgacatt acttcccata aagacaagat tttcgtaact
    44581 tgtttcgctt tgctgatcgt cttcacatac tttcgcatgc ttttctactg gatagctttc
    44641 ccctgtcaat gctgcttggg aaatgaataa atcctttgcg gacaataaac gcatatccgc
    44701 tggtatcata tcccctgctg aaagccgaac aagatctcca cagacgattt cttccatagg
    44761 aatctctgtg tactttccat tacgttttac agctgccgtt actttcacaa gtgatttcag
    44821 tttttcagcc gcttgattgg aacgtacgga ctgaatcaac gtcattgtcc cgctgacgat
    44881 aaccatagtg aagataatgg ctacaccgaa aagatcttta tctccaggag ctgctaatac
    44941 atattccgtg atgaatgaaa taattcctaa tgcgatcaac actaatgtaa atggcgtgat
    45001 gtatgctttc aatatttcta caatgaatgg tgtttttttg ccatatgaaa tcgcattttc
    45061 accgtaattt tctcttgaac gttcgatttg ttcttctgtc aatccatttg ctgatgcatg
    45121 gaatagttca aataattctt gtttcgtttt tccagcaaaa gctgcataga ttttttgtgt
    45181 atctttcttt ttttccatct caatctctcc tttgtgtcca aaagacagct gttctcgctg
    45241 cttttggtct ttagctattc atcggatcga tagagaaaaa actgaaaggg gctgagaaca
    45301 gatagataat agccctggcc tttcctctag atccttgttt ttcgaatgac tgtcatccat
    45361 ttgttctcat ctcctttact aagtaatttt aattcatgac ttccaatcat ttttcgaata
    45421 gttatttttc caattagacc acctcctttt tatttttcac cgatggggat ttgcggcaac
    45481 aaaaaaacct ctcgccaaat actcagcgaa aggtttgaaa aagcatatta aaaaaagctt
    45541 ctcagatctc atcgttgagt tttgacactg tacaacgtaa aaagcagata cctgctttta
    45601 gctaccttat gaaaagcctt atctcgacaa ttcctgttct acccattggc gtctctcgac
    45661 gtttttgggc agtagcctat gtttgtatag gagcctcacc taacaaatct tattcggttt
    45721 tccttcgtta atataacaac taaaaaagag gatgtcaaat caattttcca tgtttttcat
    45781 acttttttta aaatacacac gttatattcc tgtctcacgc ggttttattt gtctcatttt
    45841 ccacctcccg ctataataga aatatagtag gaaacgaggt gctgttatgg aaagaacact
    45901 tgttattatc aagccagacg gcgtgagacg ccatttagtc ggaagcatca tccaacgttt
    45961 tgaagcaaag ggactggcca ttgctgaaat gaagtttgaa actatgactc ctgaattagc
    46021 aaaagaacat tatcagcact tgactgagcg cagttttttt gatgaactga tcgattacat
    46081 gacttccggt cctgtcgttt atcttgtgct ggtcggcgag gaagtgatcg atatcgtccg
    46141 taagatggtt ggagcaacaa aagctgccga tgctgttcca ggtacgatcc gaggagacta
    46201 cgcgctgccg gggactgaaa acatcattca cgcttctgat tcccgtgatg ctgcggtaaa
    46261 agagatcgcc cgctttttcc cgccattcga tataaacaaa ccgtctgaaa aagcggcgaa
    46321 gtgatacaaa ttctaaactg ctggtctagt tgctgggttc agcaatggcc agcagttttt
    46381 tctctaagga cctccgttga ttctttccta acatatattc gagcactgtg aagaattctt
    46441 ggtagtcttt cgtcttttct tcccctaaag cagcagaaat caatctttga taataatctg
    46501 ccatcaatgt ttcatagatc actgtacttc tttctttatg tgataagctc cgtttcatat
    46561 cttcattcac actacgtgcc tttttcaacc gaccattttc tatataaaac gaagtcaggt
    46621 tcattaaaaa agtaaagaga tactgctcat gattgttcaa ctgtgctaat cgtcgcattc
    46681 tttttttgat tttcaaataa ttgttctcaa tgaaccgatc actgaatatc caaggaaaat
    46741 cagctagaat agatagttca aattgaccat agtctggtat ctgcatcaag tattcttcaa
    46801 tgattcggca ttcataacgt aaaaattcaa attctgtctc agatacttct gttttttcga
    46861 ccatgtgaag attcaaaacc aaataataca gatcttctgt tttcttaaaa tgcctacggt
    46921 tattgctgat ctctttataa ggatcaaagc tgtcttcttg tacccgatct agcattcgtc
    46981 tttttttctt taactttatt ggttcatcca gctggtttcg catccattca tattctgcaa
    47041 gtgtcatgtt cattcgctct aaaaatgatc tcaatgttac aaatgacatt tttttcactg
    47101 aatttgtctc aatcccgact agagaagtct taaaacagat tccttccgct aattgggact
    47161 gtgtcaactt cttgttgact cgaagtttct tgatcaattc accatcatac ataatagttc
    47221 tcctttttaa tttaaatttt tccacattca ataaatagat acgttaaaaa ggaaaaatac
    47281 acgaaaaaaa taaaaaaatg tttttttcat aaaaaatacc aaccttcaag cattagaatg
    47341 ttgtctaact actgaaggtt ggtttgattg tttattgatt atttttttgc tgttcaacgt
    47401 ctgttcttgt tactggatat aagttggttg ttgttttatt accatttttt gaaaagacac
    47461 ttgtcgaatg ttcgcctttt tgcttttcga ttgcttcatt acgttccgct tcatttttat
    47521 agtcatattt tttcggatca atcgtctcaa atccgtttgg cacataaaac cgcaagagat
    47581 tttcctgatt cagcttatct gaaagactta acgcagtctt gactttttcc tgatctttgg
    47641 agatttcctc atcgattcct gctgctttcg tatcgatgat ttctcctgtc tgattattgt
    47701 aagcttttcc tcctaggacg gtgtatttgg gtgtcacaaa gttcccattt cgaaaagcaa
    47761 ctacttgatc atgctgagaa gataataagt ctgtaccaaa atgaagataa tctttatcat
    47821 cgattcccaa tagatgtagt aatgtcggca aaacatcgat ttctccaccg tattcatgat
    47881 tgactgtccc tttagtataa ccaggcatat ggatcatcaa tggcactcgt tgcatctgtg
    47941 cgttgtcaaa ttcatcccat gtatctggat ctttaccaag agcgcttgct aaatctttat
    48001 tatctgtatt cgagatccca aaatgatccc cgtaaatcac aaagattgaa ttttggtaaa
    48061 taccagattt tttcaaatac gtaaaaaatt gttccaatga ttgatccaga taatgtgccg
    48121 ttcgaacata attatctact gtgctattgc cagtattcaa agatggaaag tcgaaattct
    48181 tgtcatccgt ataataaggc gtgtggttgg ttacggaaag aaatttcgtg taaaacggct
    48241 gttgtagatg ctcaagatat tgggcagatt ctctgaataa atctttatct aatatcccat
    48301 aacctaacgt ttcgtcggat tcatcaaaat aagatcgatc aaagaaattg tcataaccta
    48361 agtttttata tacatgatct ctattccaaa aactagctac attcccatgg aaaacagcgc
    48421 ttgtatagcc ttgcttttgt ccaaggatcg ctggtgcggc ttgaaagaca ttgtctgacc
    48481 caagttctgt gaataatgag ccttgaggta aaccaaatgt gccagtttcc aacatatttt
    48541 cagcatcact tgtctttccc tgtcctactt catgaaagaa attgtcaaag ctgatcgttg
    48601 cttgattctg gaaaatacta tttaaaaacg gtgtgacttc ttgtccgtct accttcatat
    48661 taatcaaaaa ctgctggaaa ctttctaaat ggagaacgat cacatttttc cctttagcga
    48721 tcccaaatgt ttcgggattt ggctctgcat aatgtttctt tgtataatct aaaacttcat
    48781 caatcccatt actgcttgca tgcgcgcgaa cactgctagt cattcctgtt ttgattccgt
    48841 cataaatagt atacgcatcg attcctagat atttgacaat atatgaacgg tcaaatgttc
    48901 gctgcagcaa ttgtggacgg ttggcttcgc tcagcgctag atttcctgaa aaaataagcc
    48961 ctgaaagaca ggtaacagcg atagccatcg gtttgccgac agggttgctt ttgataggta
    49021 ttttcttgac cttcaaccaa accagtagcc caatgaacac agcgatatcc agccagtaga
    49081 tgacatcatg gggcttcatc aaagaaaagg agcttcctga caatccttgc gacactttcg
    49141 aaaagcctaa tacagatttg actgtaatga aatctgtgaa ttcccgataa aaaataatat
    49201 tcaagtacaa caaaagcgta tttaaaatat caatgatcaa aagaatagct aacgctggct
    49261 ttggcttttt aatatacaaa gaaaggccaa aaaacaataa ggtagtcgca attgggttga
    49321 tccacaagat aaagtactgg atcgtacctt ccacacctaa tgaaaaatct gcatagtagg
    49381 caatgatcgt cttcagccag aaaaagaaaa cagctgtcaa aacgattgct aaacgatttt
    49441 ggcacaattt tttaagttgt gtcacgtata tcacttctct ctattcacat tttttcttat
    49501 tttgctcctg aaaaatgaaa atttcctaaa aaagttgtta tcttaagatt tttttattct
    49561 ttctaaagat tattccgtcc acttttcaca gcataaaacc gctgcgatgt aagcgattaa
    49621 actaatgaag agataaaagt cttgtggtga ccgcttcact ccacggtatt caaaagccag
    49681 tttctcggta ttcgtacaaa aaaagaggct gtgacaaaat tcttgtcgca gctccttatt
    49741 tcttgatgca ggacagcttt ttcacaactt cttccttaaa cggtgttctc ctgccaactc
    49801 ctcggccaca agtcacaata tttataaaac atgagaagct gttttttgaa attgttcctt
    49861 gtggctcaga gttaaacggc aggctcaaga cctcttgata cacggtgttc aatcagctga
    49921 ccctcggtat tagattatgt caggacgcta attttacaac ctcttggtaa acggtgttca
    49981 aaagcttgca tctgcggtaa taagcccaac caaacgaaaa atataaggag ctattttttg
    50041 aaattgctcc ttgtggctca gagttaaacg gcaggctcaa gacctcttga tacacggtgt
    50101 tcaatcagct gaccttcggt attagctcaa aaaacggcaa aatatgagga actattttgc
    50161 cgtttgtttc tctaatactc aggtcaaaac gctgatttca caacctctgt ttttcattgt
    50221 ccataggatt gatattgtcc ttgtgatgac tcattcgtaa taattgaggc ttgattttct
    50281 tcacttcccg ttatcgtttg tccactgcca gaatctgaag gacttgttcc tgtcgactcg
    50341 tttttttgag aagagacagt tgttttcttg acttgttcat ttgtcttttt ccattccgct
    50401 aaacgatcgg cgaccacttt tttgtctact ttttcagaat aagcggtgat cacttttcca
    50461 tttttcaaac gggctacttt gtcgccatca tgagtcaaaa gcaaaacggt caaatgcttg
    50521 tctttaaatt gatattgtct gagagaaatc gcactaagta cgtcatcgat attaataaat
    50581 tgattttctt cttttccttt tggcaaataa atgtccaatt cgcctttttc gtaattggca
    50641 gttattttat actccgccca ccaataagtt gagaattctt ttttgaaatt atctactgat
    50701 tctttgcttg ctacgatttt ttctgtttct ttctgagcaa gatctgctgt catttttcct
    50761 gaaaatgctg cggataaaat aagcagtaag aaaaaaccga ataaccaaaa gagaagaaac
    50821 ggtctttttt tcctcatgct ttcctccact cctatttaat tttgactatt ctattatata
    50881 caaaaaatga aatagaaaga caaaaaagtt gaaaaagatc gtaaataatt gaacttcttt
    50941 ttcacaaaat ggtaagaatg gagaatttca cgcatttgct ctcaactatt ctttcttaag
    51001 cctcaaatcc gtttttatct gatacgtctt tgaaccaatg acccgatttt ttgattgttc
    51061 tttttccatc ttgtgctaag tctactgaaa taaagccata tcgattctta tatgcattgg
    51121 tccacgacca gttatccata catgtccaca tatgatagcc taaacaattg gcaccttctt
    51181 gaatggcttg atggacatat ttcagatgag attggacaaa ttcgatccga taatcatcgt
    51241 ggatcatgcc atcttctttg ataaatcgtg cttcgttttc tacacccata ccgttttcag
    51301 aaataaagca gtcgatattt ccataatgat ctcgtacatt gatcaaaata tcatagattc
    51361 cttgttcata gatctcccat ccgcgataag gattcatttt acgaccaggc atttcataat
    51421 tgtcgaaata atcctcaggc attggaccgt ttttagtatc gattggtgtt tcttttgctt
    51481 ttacccggcg tggctggtaa taattgatac ctaaaagatc tactgtattt tctttgataa
    51541 ccgtcaaatc ctctgcatgg atcacaggca tgagatcaag ttcttttact atctcaatca
    51601 attcacttgg aaactcgcct tttacagctg gatcaaggaa tgaacgattg aagaacgcat
    51661 ctgctatttg cgctgcttta acatcttcag ggtttgattc gtctcttgga tagcttggtg
    51721 tcagattcaa gatgatacca ataacaccac cttgctccat ttcatggtaa gatttgatgg
    51781 ctaacgctga agccaacgct tcatgatagc caacttggat cgcttttttc atatctgact
    51841 cttctggata atgccatcga tataaataac ctgcttcgac tggaacgatt ggttcattat
    51901 gagtaaacca tttttttact ttcgaaccaa acaattcaaa acaacttttg gcatattttt
    51961 cataggcttc aaccgtttct cggttcaacc atccgccttt ttcttgcaga tacatcggca
    52021 tatcgaaatg atacagattc acaaagggtt cgataccgtt ttcaatcaac tcatcaaaat
    52081 aacgatgata gaaacgaacg gcttcttcgt tgattgttcc agattcatca ggtatcaaac
    52141 ggctccattg gatagatgtt cgaaatgaat tatgcccagt ctgtttcatc aactgtacat
    52201 cctcttgata cttcgtataa acttgcgagg ttttttcagg tcctacttga tcaaagaact
    52261 tttgcggttc ttttttgtac caataatcaa aaatgttctc tccttttcca tcgcctgaaa
    52321 tccttccttc tgtttgtggg ccacttgctg cagaacccca ccaaaaattt gctggaaatt
    52381 gatatttttt catcaaagca cctctttttc ttttttagtt cgttttagtt gattccatgt
    52441 atgatatttt ccatcacaat cactttttca ttttcttcct cttctttttt gattcgtttt
    52501 aacagctgtt ctgctgctcg ttccccgatt ttatctgttg gtggcgtgat cgtgattttc
    52561 ggaaggatac tcagatcgtt tttatctaaa taatcatagt gactgaacgc aatatccttc
    52621 ccgattctga caccttcttg attgaaacat tcgatggcac caatcgacat attgtaattg
    52681 ctgacaaaaa tagcagttgc ccctttttct aataatgctt ttgccccgta atagccgctt
    52741 tttctagaat agtctcctgt aaagacctct aattggatcg atggatcatt ttggaccgct
    52801 tctttggctc ctgctaaacg ttctcttgct acgtagtctg tttgagtggc agcaataaat
    52861 ccgatttttt tatgtccttg ttcaccatga tgagcgatca cttttgctac actttctcga
    52921 tcattcacta atattgtatc aatacctgct ggtccttctg gacaattcaa tgcgataact
    52981 ggaatactca tttttgtgag catttcaaat ccagaccact cttcttcgga taagaagaca
    53041 atcagcccat cgatcacatg cttcatcagg tattcaattt tttgttcaat tttttctggc
    53101 tgatcggcaa acccgcttaa taataagcta tacccatttt cttcagctac tctttcgatc
    53161 cctgagacaa cttccgaccc aaaacggctg gaaatcccgt ttatcaaaag tccaatagag
    53221 aagcttcgat tattcttcaa ccctttagca atgatgtttt ctttatagtc caacgctgaa
    53281 atcgcttctg ctattttttt catgttcttt tccttcagtt gctgaccatt caagtaacgg
    53341 gaaactgttc ccactgaaac accagccatc ttagccacat cacggatcga tgccatacat
    53401 tctcactcct gccagttaca tttgatttct ctttcttgtt gttcgattat gatgaccgca
    53461 ttctttgcat agtgttgctt gtcatcaata tccatataac taaagtcttc agtagagcaa
    53521 cttcctctca ctattaatta tacttaaaca agtatttgaa ccggttcatt tttaatatag
    53581 cattttttta aaagattgca acactttttt aaaacgcttt ctgaaagtgg cggatttttt
    53641 gcctttttaa aattttattc taaaatttac tctatttgtg ggtgttttgt gcgtttttct
    53701 cttccatccg tttgaagttt tacattccaa tggtataatt tctttatcct atcaaatggg
    53761 ggaagtattt tgaagaaatt aatcgtacta tttattttgt ctgcgggact tttgacattg
    53821 actggctgtg atttgtttgg cggctctgac caatcctcgg acgaaacaac cgattcttct
    53881 gtcttaaaag cggaaaaagc agataccacc aacagtgtat atacgctaaa agtacgtaaa
    53941 gatatggatt tatccgcggg acagttgaca tttacagata aaaaaatgga atggacacgc
    54001 acgtatcaga cacaaggaac tgattcttct gaatcatctg aagaaaaaac agtgctgaca
    54061 gatatcaaga tcactaccaa agatgacgaa tatattattt caggaaaaga aaataaaaaa
    54121 gaaacaacag tcatatttac aaaaatcggt aattaccgtt tgaaggatga ggatggcaac
    54181 atctacagtc tatagtgccg tctgttggat cttcttttgt ttctcccctt cttttctgct
    54241 atagtaaaga agcaagatga ttgtaaagga gacgaaatgg atgatcgata gtcctgatta
    54301 ttgtacatgt aataacaaca aaaatatcca tacagaattt aatgaccaag gaagcttgag
    54361 catatgcgac aactgtcaca aactagtcaa agattcattt aaaacatttg aatcttatcc
    54421 tgttcaaaat tatgtaaaaa gtagaagaag ataaacaaat ccaagcaaaa ctcactatgg
    54481 ctgattttta tgaacttcaa actcagcttc ttcctttgat caagcttcat cagtaaaaca
    54541 aacagtggat catttcagtt tacaaatcaa agagtaaaga tttatttatt tcaaatattt
    54601 gaatataaaa taaccaaacg atagaaaggc ttattctaca tcacgtagta atttttcttt
    54661 cccgtcgttt ggttttttat ttttagccta attcttgacc ttttgcatgc tggttatccg
    54721 taaaatattt tccagcaggc tttccttcca attcagctaa tttttcttgg atctgacgga
    54781 tcaccttttt tgcatcttct ggattttcta caaaatcgaa tttatcccca tccacttgta
    54841 ttttaggaca tacatcaaaa tcttcatacc attgattata gcgggtgttc aacgtttcat
    54901 aataggtata taacgtctca tcgctttcta gctgttcata gtcacgccca cgttttttga
    54961 tacgttctag catcgtgtcc aaagaaactt tgatatggac catcaaatca ggccgttttt
    55021 ttggagcaac atcatctaac tcgtttagca tcgtatctaa taagctatcg tactgctgta
    55081 cttcgatatc cgttacccga cctaaatcag cgtttagatg gaacaacagg ctatcttcat
    55141 agatggatcg gtctagcaca ttgtcatcgt tcactaaagc atttttcatt gctaaaaatc
    55201 gcttgttcaa aaagaagatt tgcaataaaa aagcgtactg ctcaggattt gaataaaaga
    55261 gcggcaatac ttcgttatct tcgacatttt cataaaatgg tttagaatca atctcttttg
    55321 caagcatatc cgtcaaactg ctcttacctg caccaatcgt tcctgaaatc aaaatcactg
    55381 acatacaacc cttcctcttc tataagtact cgcctacgta agtttcaaca ggcaatgttt
    55441 caaaatcata ttcacgtaca gcttggtctg attttaaata cgtatggaga ctttgtgtaa
    55501 tcatcaagtt cgtaggtacc gtctcttctt gtacaatgat catcggtttt ttcaacatag
    55561 ttgccacacc taattcgtaa gccgttcctg gatcgattgt ttgatgctca aagtcaagga
    55621 tagcaacaac aaattctgca tttgtcagtt cttccatatc tttttcgtat acttcctgcg
    55681 cccatccagc gctgaatagt tcataattgc tttcttggtg atgacgtgga gagtagaaac
    55741 ttgtgactgt tttgttttcc tccaacgctt tttcgatacg acttactcga tcgatttgct
    55801 cctctgaaaa aaatggtcct gctaaatata tttgactcat tattttgcct ccttcaataa
    55861 taactgattt ttaatagata ctgctcgtaa ttcattgacc agttccaatg gattgttgga
    55921 aaagattact tgatccaacc attttttatc cataaaatcc tcttttgcaa aagtttctaa
    55981 taattgttgc aagccgctgt aataaccgcc aatattcaat attccgattg gcttgtcggg
    56041 cgtcagaccg attgctatcc aagaaagaag ttgtgagaat tcctccaatg tacctagacc
    56101 tccagggaaa acgaggacag catctccctt ttcaaataat aatacctttc tttcatcgat
    56161 cgtttctgta ggaataaatg tagtaacatc ttttccaggc aattcagttt caaaaagccc
    56221 attcggatag atacctgtga cttttccgtt attttccagt actgttttac taatcactcc
    56281 cataatcccg gagtatgaac caccgtaaac cagttcgatt tcttcttgtg ccatgtattt
    56341 tcctaacgtt tgcgcaataa atttgtaact ttctttatta ccaaaacggg aaccgcaaaa
    56401 aacagtaagt ttcataaaaa aacactccct aactcaaaag tctatcttca ttctacacgt
    56461 gtttttttaa aataaaagac tagattttgt agttttttag tggtatatag gatagacgcc
    56521 actatatata gtatttagaa aaaaatcatt tttcagccat tttatagata aaacaaaaat
    56581 aaataacgtg ttttcatcga tatgaaatga cttacttata gaagatactg ggatttcgtc
    56641 ctatatgcca ataacgtgta ttattttaaa caacaaatac ttgtttcttt tattaacgaa
    56701 aaaatcattt tgttctcgtg ttatgtttca ctgataaatg atgtttctct aaataaaaaa
    56761 gccgacgaac taaacgatca aaatttgatc atttttgttc atcagcttct cctaaaaatc
    56821 tttttgttaa cgataaatag ctggatttgt aaaaatatca cttgaatcgt cactgctgga
    56881 agaaaattca gtgataaccg cacctgttgc gcctgcctgg aactagtgtt tgatattggg
    56941 ctcgaacgtg tactgatctc tagcatgaag aataatttct ttttttactg tataataact
    57001 ttctttcctt tttggaatag tacaggaaag tttttttgtt tcctttcctt cgacataagg
    57061 tataccagac cctttcgaca acgaaaggtt ctcatttttc cttcatcgtc atttcaagga
    57121 gggtgccgat gctctggaca gtttcgatcg ctcctaaacc aaaatctgta tattcgattc
    57181 tttctaactc tgattctttc ataccattcc tgtctcaagg tacatctatt gtactttttt
    57241 cttgtaatcc attctgcctc tcctaactaa ttgtcaaatc atcccaagat atgtcttctt
    57301 cctctttttc ttcttggatg atttctgcat tcggatctac tggttttttc aataataaaa
    57361 tggttattcc tgtgacaaaa gaaccagcta acaatgcacc aatgaatgcc catggtctgg
    57421 tcatcgtagc gactgcaaac attcccgatg ccggaatacg gctatctgcg ccccaaaaca
    57481 tgcttaatcc gccacctact gtcccaccga ctcccgttgc tatgacattg cgtgccaagt
    57541 cgttcaatac gatagggaaa catccctctg tgatctgaca gatccccata ggaaaagcgg
    57601 tcttcaatgt atcgatttct tgacgattga gtatattttt acgaatgatt ttccctaaga
    57661 aataagcgat cgtcaagccg aatggggtta ccatcgaagc taaaatcaat acagtgattg
    57721 gttcatagat tccttctgaa aatcgtgcca caccccctta tccagaataa atggtaatcc
    57781 agaaacagcc gtgtatctgc ggtaataagc ctaaccaaac gaaaaatatg agaagctatt
    57841 tttgtttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct ctttttttat
    57901 ttatgtttca taatcaatat ggtaaggatg cagctccgta tggtagaccg cctccgaata
    57961 ttcaatcatt tccccaattt gcgtagacga tttccgtatc cgtttgatgc cgatctgtgc
    58021 gtctgttttc agaattcctt gttcttcttc tgttaaaaat actgccgaga aatcatcaga
    58081 aaatgagtca atagtatatc cttttttcgt cagcaaacga tacaaagatt ctttgccaaa
    58141 ttcttctctg ctgaattgca tcatttcttg tggaagataa tgaacaaaat aaatataagg
    58201 ctgattgttc aaataatata agcgcttaaa acaaacagct tgctcaccga aaaaggaaaa
    58261 caaaggatct tccttcttta tatggaccaa ttgcaaatcc agcgtttctt tccggacttc
    58321 atatcgggag ttctccaaga tttgcgtaaa gcttgccgct ttggataatc gattgtaagg
    58381 acgattgctt agaaccgtcg tcccacgacc acttttcttt gcgacatatt catctgatgc
    58441 cagcagttcg attgcctttc ggacagtgat tttgctgact tgaaacagtt cttctaattc
    58501 actttctgtc ggcaacatcg aaccgactgg atattttcct gagaaaatat cttctttaat
    58561 tttatctgcg acttccaagt atagtacacg tgtccttttc ataacgtcac cctttgcttt
    58621 actattacta aaagaatagc acataaaaaa taaaaaaaga acacaaatca catgtgagaa
    58681 gtgtgttctt tttcattcag ctgctttaat tacaagttta cgccatttga ggcaattact
    58741 tctttatacc aatcaaaaga ttttttcttt gatcgattca gtgtcccatt tccttcatcg
    58801 tctagatcca catagataaa gccgtaacgt ttactcattt ctcctgttga tgcactgaca
    58861 agatcgatac atccccaagg cgtatagccc atcaaatcta caccgtctgc tactgcatta
    58921 gccatcgctt cgatatgacg acgtaaataa tcgatccgat aatcatcaat aatcgtgcca
    58981 tcctcttcca tatgatcaat ggctccaaga ccgttttcaa caatgaataa tggtttctgg
    59041 taacgattgt ataattcatt caatgcgatt ctcagccctt ctggatcgat ctgccagccc
    59101 cattcacttg cctcaagaaa cggatttttt actcctccca gcaagttccc ttctgaagtt
    59161 gctgcttgtg gatcagtctc attgattgcc gtagacatat aataactaaa tccgatgtag
    59221 tccactggat attcttttag caactggaag tctttttccg tttgctctaa tttttccgga
    59281 tcgactccat attttttatg ggtacgcgct gtgtatgcag ggtattctcc acgtacttgg
    59341 acatccgtgc agaaaaaatt gaattcttgg ttttggatca ttgttgctgc ctgattgact
    59401 ggattggcat cgatgctgta agttgttgca tagataatca tgcagccgat ttggatatcc
    59461 gaacgcaatt cgtggccgat ttttacggct ttgctacttg caacgaattg attatgccat
    59521 gcttggaaaa tattttggta ctctcctgct ccattgcttt ttactaatcc ttgacttaaa
    59581 gctggaaagt gaaaggccga attgatttca ttgaaagtca tccaatattt gactttactg
    59641 ctgtatctct ctaatactgt ctgagcaaat cgttcataga agtcgatcag ttttcgattt
    59701 ttccagccac cgtattcttt tgccaaatga agaggcattt cgtaatgaga aatcgtgatc
    59761 acaggttcaa tatcatattt taagcactca tcaatcagct gatcataaaa ttctaatccc
    59821 gcttcgttag gagtaccttc gtctccattt ggaaaaattc gtgtccaggc aatcgaaaaa
    59881 cgataacatt tgaatcccat ttccgcaaac aaggcaagat cttctttgaa acgatcgtaa
    59941 tgatcgattc ctcgatgatt cgggtaaata tatttttctt gatcaatcgt ccagtcaaat
    60001 tcttcactgc cgataatcgc aaagcgctgc ttacctccag gcatagcatc tgcaacggat
    60061 agtccttttc cgtctcgatc ataagcccct tctaattgat ttggcagcag ttgctccacc
    60121 ccataaaaat ttttctgaaa aacgtgattg catcctgatt tcctccttat tttacaattg
    60181 ccagaacatc ttcgtttgca ataatctctt tttggtctaa ttcaagtata tcggcaaatg
    60241 catcagtatt cgtaatgaca actggagtga ttacgctata gccagctgct ctaattgctt
    60301 cgatgtcaaa acgaatcaat aaatctcctg ctttgacgag atccccttgt ttgactaaga
    60361 tctcaaagcc attcccattt aattctactg tatccatccc gatatgaatc aaaatctccg
    60421 ctccgtcagt cgttgtgatc cctacagcat gtccagttgg aaaaatagtc gtgatctctc
    60481 cgtctgcagg tgcaactagt tctcctactt ctggatcaat agcgatacct tttcccatca
    60541 cgccagaaga aaaaacttgg tctggtactt cactcaatgg cagtacttta cccgttaaag
    60601 gactttttaa ggtatgccgt gctgaagtaa tcggttctcc tgcattcgca tgtttgtttt
    60661 ctaattgatt gtcttgtgtt tgttcgtcaa atcctaaaat caatgtacct acgaatgcaa
    60721 gaacaaaagc aattcctgta gctattactg ctactgtcac atttgactct acaccatcca
    60781 ccgtattaat gaaagtcgga atcgtcaata agctgaccaa actgctggag aaagctttta
    60841 ctccggaaaa tccgataata gctccaccaa tacctccaga aatacaagcg gcaatgaatg
    60901 gtttcttcaa aggtaatgtc acaccataca cagttggttc agtgattcca aataacgaag
    60961 tcactgtaga agaaatccct aatgctcgaa gtttcgtatc ttttgttcgc aatgctacag
    61021 ctaaagcagc cccaccttgt gccaagatgg ctggaagaag cattggcatc aatacatcaa
    61081 acccatattg ttcgatattc aaaaacatga tcggtacgaa tccccaatgc atgccgaaca
    61141 tcacgaatac ttgccataaa cttcccatga ttagacctgc tacgattgga ctaaatccat
    61201 aaatcgagtt gaagagaccg cctaaagcat taccggcaac cgtcccgatt ggtcctaaag
    61261 caagaaatgt taatggaacc atcactacta aaacgacaag cggaactaaa atcatttgca
    61321 gaaattgcgg aatcactttt tttacgaatg gctctaattt actttgaacc catacggcta
    61381 gaatgattgg tatgacagaa gaagtataac cagatggtga aagaatcact gggatcccaa
    61441 agaaactgat agcttctccc gcaccagcaa ctgctgtgat agctggatac acaagtgcca
    61501 tagcaattgc tactgctaaa aactggttgg ctttgaactt tttagctgct gtaaaagcaa
    61561 gcatgacagg taagaaggta aagacaccat ctgctgcagc aaacaatatt tgataagtac
    61621 cgctatcagc tgtcagccag cccatcaccg ttgccaatga taagaatcct ttcaataccc
    61681 cggcggctgc catcgcccct agaaaaggtg taaatatact agagataata tcgatcagac
    61741 gattcaaaat atttttggac tcttcttttc catcctccga aactgcaaga ccttcctcat
    61801 ccacgatcga ttgataaaca tctgctacat tactgccaat cactacttga tactgaccgc
    61861 cactttgtac gacttggatc acgtccgggt cttcttttaa cttttgcgta tctgctaaag
    61921 attcatcttt taatctgaat cgcaaccgcg tagcacaatg tactaaactt ttgacattct
    61981 tttgtcctcc taccgcttcc catacacgac gaccaactgc ttgattatcc attgtttttt
    62041 ccttctttct tttttattta aaaaataaaa acccagattg aaaaaaacag tcacaaacat
    62101 tcgtgaacct attttttcaa tccaggtttt gcctgccata cagtaacaat ccttatgatt
    62161 ggatgctttt tttctcactt actactcttt gaatatgaat cgttaaataa atctgctctg
    62221 cgatccccat ttcctgtttt tgcgtttgtc gaagatactc attgatccgt tcgacacaat
    62281 gaaacgcttt tggatacttt ccttgaacta atgcgtagag aaattcatct tgctcgtcag
    62341 atccttcccc tttgagataa cgctgggcaa aaaactgcag atgagtaatg atacgctgat
    62401 aattcaaaga ttcttcatcg aacacgatgc cgaagaattt actgatgata ctcagtatat
    62461 ctcttaccat tgtggtagcg gtcatcgtca cttgcatgtc ttcgtttcct tgttggctat
    62521 tcacaaaatg aaaggcaata aaaccggctt catcctcaga aagttccaca cccgtctttt
    62581 gtttcacgaa caaaagagcc tctttcgctg cttgatattc tttcggataa aatttttttg
    62641 tttcaaataa taatggatta ggcagttgga tattttcttt tgctcgtgtg atcgcataat
    62701 gaagatgatc ggtcaatgtc agataaatat taggcgaaaa ttcaactttt agtttatctt
    62761 cagccagttc gactacttgt ttgacccatt cgatctcctc tgccggaata tcagccatca
    62821 gctgctggaa ttggttttta gcaaaagaat ctcttaaaca aattcttttt ggaccgcttc
    62881 tggcgaaatc tcgtccccaa tctttttttg aaaagctagt ccttttccca tacaaacgac
    62941 ttcttccccg aatccatttt tcgaaatgac gacattgtta ttcaaaattt tatcaatttt
    63001 cattttgctt cgccctctca agaaaaataa aaaaacctag aattcgccta ctgtttctta
    63061 gctgaattct ggttttgcct gccgtacagt aacaatccat ttcttattca aaatcatcat
    63121 accatcgaat ttttatttat gcaagccttt tcatataaaa aataaaaaaa ctggaacaca
    63181 agtctatcta gaaaacaacc aaatcagaga tgcacctctt tgcgtttctc tttttttcag
    63241 caaattggtt tttccttatc gattcatatc ctttggctgt ttttaatctg taacgactta
    63301 tgtcccagta tcctaaaatg tttttgtctc tagattgaaa gcagatctca tatcatctgt
    63361 cgcatctttt atcgatgtaa cactttccag ttctccttgt acaactagtc gcgtgacggc
    63421 gtccatgtcg ccacaagtaa ttagtgtgac aatttctttt ccctcgactt cattcagtaa
    63481 ttgggtatct gtaggagcaa ctttttcttt aaaaaatgtt ttgtatgtat acacattttc
    63541 taaatctgtc aaatagatct tggcacccat ctctaaattt tctagcggtg taaacagcaa
    63601 ttcagggttc gttgcacgat ggcttgctaa ggcataattt ccttttccca tttcttgatc
    63661 tggagataat gttcctgctc cgtaaagtag tgacgtatta tccaagcctt tgaaaaccgg
    63721 taaattgatt gaaactgaag ggactgcgat actagcaatc actggtagat ctgttcctct
    63781 catttgcgac ctcatgaccc cttctgtcgt cattggttct accgcatcaa agtcaaaact
    63841 gacatcttta tcgttattct ttttcagatc ttcttttgtt acattcgcta tagcatattt
    63901 atctcctgtc tctctgacaa aataatcctt gatctgttca ttgaaaatca aagctagtcc
    63961 tactaataac aagaggaaca agaggatatt gactagccat ctttttgctt ttcctttttt
    64021 tttcattttc tctccctcca gactcatttt actgtttttc ttttgctctt tccaacagcc
    64081 gaatgacaca tttctacaac tctgtgtatt tttttcgtac acctgcgctt ttttctacag
    64141 gatatttcaa attatttttg atcaattttt cttcaatgat ctcatctaga tccaggtgaa
    64201 ggttgtctgc catcatgtat gaatagatca atacatctgc cagttcttct ttgattcgtt
    64261 ctaattgtgt ttcttttact tcttccggcg ttttccattg aaaaagttct aacaattcag
    64321 atgcttccaa agaaatagag atagccaaat ctttttcgtt atgaaattgt cgccaattac
    64381 gttcatctct aaactggttg actttttcca tactattcat ttgcttgcct tcttccaatc
    64441 acttcttact tgattaatca taccattttt ttacataaaa aatagatgat gcttttaaaa
    64501 aaactcctgt tttcttttat ttaaaataaa tgtcatagaa aatcaataat ttaggtagcc
    64561 accatgtgta tgcagtagcg tgtcttcttc ccttgaggct ttttgttttt cgatacgttc
    64621 ttcttcccaa acagacaacg ctactagaat ctctcttaat gattttcctt cttcggtgag
    64681 cgagtactct actttaggcg ggacttggtt ataggctttt cgatggatac tttcatcttg
    64741 ttctaactct cttaactgct gggtcagcat tttttttgtg atcgtaggga tttgcttgct
    64801 tctccacgcc taaaataagc gatattgctc ttccctttat ctggtgtaag caaagcagtt
    64861 agcattttga cgatcgtggg ttccgctttt cacttcttat ctacttagta tcatgaagta
    64921 tactttcgta ctaatactaa gtagcggaag tgtcaactac tttgtttaac tacatagcaa
    64981 aagaaacaga agggtgacaa cggtcatcac gcttctgttt cttttgaata tgctgtgttg
    65041 ttgtctctat ctccgtgtag actttcctca aacttatcct ttgacagtaa gtcagtaggc
    65101 tgatttgctc tttctagatg ccagatacat ttctacaaaa aaatagtgat tctgttttga
    65161 aaattcgatt tttagttctg cattttcgta aacatccaac atttttttcg cactgtatag
    65221 acctaacccg ccattttttt tagaggtttt tcctcttttg accatctccg atagcgtcaa
    65281 attcgttgga tttgccttgt tcgctatact aaaacgtgat ccctcttttt cttccagcag
    65341 attgatcgta atcggactct gatcggctac acgatgatcg aatgcgttag aaataaaaat
    65401 agataaacag cgaataaaaa caaccagatc aatcgggatt gttttcactt cgacaggaat
    65461 aactacttgg atcgggattt tctccgattc tgctttttct agaaatttta aaaagacacc
    65521 cctgacagct ggggatacaa tatttgttag ctgttgtatt tgcggttttt caaaaatagc
    65581 tgcagaatga tcttctaata tttctaaaaa ttccaacgtt tcttttttat tttcctgaag
    65641 agtaattttt aatgaaaaca gtagactttt ataatcatgt ttgaattcct ctagatgatg
    65701 gtaatattct tcatttattt gatacagttg aagcgattcc tgatgttgtc tttcttttac
    65761 ttggaaactg atcaacataa tggaaaaaaa caaagaaaaa aaactgatga ctatcgacaa
    65821 aagagataga agcagataat tagatgagcg gaagtgaaca cttcccaata ccaagtaaat
    65881 cacgccccag cttaataaaa tacaaatacc tccaaaatag tgttcagttg gataagtaaa
    65941 aagcaagttg agaaatctcg tctgtttgtc tatacttttt actaatagat agaaaataaa
    66001 tgcgataagt agtgcaaaaa aacttgctgt tccaagatgt ctctttccta aataatccat
    66061 cagatcaaaa accagaaaaa agctgagtcc agtgatagca aaaaaaagat tactgatcaa
    66121 acagctgata aacaggtttt tcgtagtaaa ataaaaaaga aacatcaata ataagccaac
    66181 taggtaagga aagctcactt tagggatagg aagtaaaagt gtcaccacca tcaaagctat
    66241 acaaccaaag ctgtttttta ctagcagttg tttcgaaaag aataaataga tacctaaatc
    66301 taaaatcaaa gtaaaggtca aataaagtgt cactgttgcg accatcttat tcctccattg
    66361 ttttgtttca aaatgtacac atattttttt aataggagcc tgcatatgaa aaagtatttt
    66421 atcatagaag ataaccagtt tcacagcgaa tggatcatca gagaattggt caatcatcat
    66481 atcgagaata tcgtgcagct cacttctatc tctcaaatca atcagttgat ccagttgggc
    66541 actattgata atcacgatgt tttctttatc gatatcgact tgaagcaatc actcacaggt
    66601 attgatgtcg ccgaacaaat acgtaaaaaa aatgcacata gtaatattgt ttttttcacc
    66661 gcttatacga attttgcagt agagatcatc aatcggaaaa tccttccgat tggatatatc
    66721 agaaaagaca ggaatttatc tgaacaaatt cgtcaagtgc tagaacagat caattggaaa
    66781 gaacagcaga tcgtaaatgt cgataaaatc tttattgatc aaaaaggtgg aagtctcgtc
    66841 ctgtatcctc gggaaatttt atatattgaa agtatcaaag gtttgaaaaa ccaagttatg
    66901 gtcaaaacaa ctacagaaga taaattagtg aatacttctt taaaacaatt gaagacagag
    66961 ttagaaaagt atgcttactt tttactttta aaatcttact gtatcaactt atcgcaattg
    67021 cagaaaattg attctgctca ttatgtgtta gaatttttca acggtgactc cttactcgtt
    67081 ggtcgaaaga tctttgaaaa gacaaaggaa cgtttccatg acttccaaaa aactgcttcg
    67141 agttgatgaa taagagctga gacgtaagca tagcagattt cagggagtgg gccttgaatc
    67201 ggctccactc cctgaaatcg ttgatatgat tttacgcttc ctcttctgta ggagcatctg
    67261 ttaacgacca tgtgaatgtt gaaacgtagt tgtctacagc ataattggat acaggaacaa
    67321 ataattccac tgattgtctc ccttgttcca cattctctcc taatgaataa atccatgtgc
    67381 caatcccttc atttgctcct gcagttacta gatctactgc ctgatctgct gggatttcga
    67441 ttccttcagc gccagttcca gaaacggtag atggcattcc actgctatta gttgcgctat
    67501 ttcgaatcac ggcgtttttg aatttcacct ttgaaccagt tagtttattc gtatcagatg
    67561 ttgtaccaat aaattctgtt cgtttgactg ataatttcca accggcataa tttccgcttt
    67621 tatccgttac ttgtacgaaa ttctgtactt gtgtttgtgc tccagtactg tccgttccgc
    67681 tttgtaatgc agcatatact gtttttgctg tacctaactt gattgttcca aaatcaagat
    67741 ctggagcttg gtcaatcgtc agtaatccgg atgttccctg atttggttcg tcaggtgtaa
    67801 aaggagtatc aggatcattt ggatcaacag gaggaactgg aacaacgtct tcaggatttg
    67861 cagcatctgg tttggtcaca gaaatcgttc cttctgtagt ggctgagctc ttattctcag
    67921 caaaaacact ttgtgtacat aataatagac ttccagctag aacggtactt aatgcaattg
    67981 tttttttcat ttctttttcc ttctttcttt taaaataaat aatccagcaa ataagccaat
    68041 aagaatgact ccaacaagta tcagccatcg ccagtcaaat gtttctggct gattttttaa
    68101 atcaatatcc ttttcattca attggtccgc tgcatcatca gttatttcaa aatcggtatg
    68161 gagattccag cgtttttctt tccaagcacc cttcacaacc gcttgataat gtcccggagc
    68221 taattttttt ccttctaatg ggacaagtaa atcaaaattt gtattgggtg ctacatctat
    68281 tttttctttt tttattgaaa ggtaaggctc ttttgtttct tttttgtaga tttcaaccga
    68341 tgtagtcaag ccgaacatat aagaagcatt tggattttga agattcgcct taatcacatt
    68401 tcttccatcg ctttgatccg cataagcttt ttttaatttc aaatctgggg aagactcttt
    68461 ttcgctattt caaatcaaga ttgctttcac ttgagaaaac tgattgacga caccaactgt
    68521 acttttgtta gaaacgttct cttttttttc ttgaaacgtg atccctcctg cgaccacacc
    68581 agccagctct ttttctggcg ctttcagtga aagtgtctca gtgaccgcac tttgaggagg
    68641 aacagttatt tctcccactg tttttgctaa gtttttcata tcataactta atgtttcatc
    68701 aaatttactt tttgattctc catattcgat cacaccgttt gaattcgtcg tagcggagtg
    68761 gatcaatgtc tcaactgtta ctgatttatc tgttcggtta aagaccgtgt aggggatttc
    68821 ctgtgttttc ccaggctcca tccttacatt tatatatgtt ttgttcttat caatctgatt
    68881 agccggtaat tcaaaatcta ccgcaaaatt gaattcggag gcatggacag gtgtctgcca
    68941 aaggatgaat ccaaggagaa ggatcataag taaagagcag ttgtttcgat gacttttatt
    69001 ccatattcga ttcatcaatc atcctccttg atctttattc tgtcggcgca tctgctaatg
    69061 tccatgttat ctctgctgag taattaccag ctatctgatt ttctttggtt aagacaagtt
    69121 taaacgcccg accgttattt aaatattctg aataatctgc ttgcacattc gttgaatttt
    69181 gtcctctgac aatttccagt tcttcttcat tcactaattg atactcctct cctttcttga
    69241 aatataggac atcctgcata gaaaatcctg catcatttgt gaaaggctga gataatttta
    69301 tcgttaagat ccacggattt ttcgtttctc gctgatcata aatccataat ttttcctgta
    69361 tatccatttg gccaaatgct tcttgtgtca aaccagttgc tttgatctga ccaaaatcga
    69421 gtttggaagg ggcaagaaat gagagaccac cttctactac gatcttcgta gcaggtgcaa
    69481 aaggcggggc atcatgataa acaactggcg tatcacttgg attgtgattc ccaatttcat
    69541 cgttggtact tggcttgttc tgcacaccct tagcagtagc ttctcctgct tggtcgttta
    69601 atacaatttc taatacatct cctgcaaaaa gttgattttc tgggatagaa agaacaaatt
    69661 cccctgtatc atcaatagtt gtttctgaca aaacagcttg attttttttc aatgaaacca
    69721 aggctcctgc ttcacctgta cctgtgatcg tgcgagaagc tgcattgatc aatgtggtac
    69781 ttaaattagc aggttcagga ggaatcacat cgactgtcgt tttggcagag tccagttctg
    69841 tcacttcttg gccacctctt acttttttag cagcaacaac ttgcacttga tcctccgttt
    69901 ttaatctctc atcattgggt acttgaactt cgaacaagcc gccttgttcg ccttctcccc
    69961 atgggttata tcctgcccct tcattttcat ctgtttgtcc ttgcgtgaga gcagtaattt
    70021 ctacagatga tttcgtcgta tctgcgtaag taatacgtaa agtcacttct acttcgcctg
    70081 tccaagcatc tcttggcttt gcctcgacac cttcgggaac aacgacatgc ccatagattt
    70141 ttttatctgc attcgttggc actcttaagg attcaatcat cgggagctga ttattggcac
    70201 tgattcgatt ataatttttc attccggcac ctgtctgagc aaattcattt gttaaacctg
    70261 cactagggct tgatacgaaa ttgcctaagt cggtcccatt gatcgataag tctgtcatat
    70321 cagaaaaata atccgggtct ttttcaaagt tatcgaatgt tgcgcctgtt tttcgccata
    70381 aagcgatttc tgaatgggcg atctttaaag aagaatctcc atttccttgg aatacccagc
    70441 cgcctatgcc attggtctcg cttccaatcc gtttgttttg gaaatcaaaa taggtaggag
    70501 attctaagtc cacattcaac acatccccac tgatgatgcc ggtcttatct tcccctgttt
    70561 tcccgaccat gactaaataa gttttttcac ctgccgtaaa atttaaatca atatctccag
    70621 ttgtatctac agttgctccg gaatctgcgc gtatatcgat atttgatttt tctccagtga
    70681 ctgtaaaatc agctgaacct ggactttcta gaaaaccaga agcgtcataa aattggatcg
    70741 cttgatttcc ttgattgagt ctgttcacgt aagatttgcc atcacctgta tttcttaccc
    70801 tcaatgagcc tccattggtt actgtcagct tttgcgtacc attctcaaaa cgaactgcgc
    70861 tggaacgccc actctgctta tcgatgtcga taactgatcc ggctccttct acgatcaaag
    70921 aaaggccttt tcctacaaaa cgcatcccat tgttcccggg ttgatcgtca tctccagcca
    70981 ttttgaccga gatttgtcca ccacttgata catggatttg actagaatct ccttgcaagc
    71041 gaaatccgcc cgtagttgct gtattgatgg tgattttccc accattcgat acagtgactt
    71101 tcgatcctga acctctcatt tggatgattc cgcgattgtt gtcattggta atattgatct
    71161 ttgtatttac cgaaaattcg cttcccgaac cactgacttc aaacatgaca ttaccaggta
    71221 gcgtatcgac tgcctctcct tcatcgctaa cgccatctgc ccgatcataa tcaagagaga
    71281 cctttgaacc gcttaagata tggattcctg gcgctgtttt tccactacca gtctcatctc
    71341 ctgaatctag aaaaccgtag aatttcccta gatacggtcc ataaagttga ctaccattga
    71401 tcacattcaa ttgatagctt ctacaaaaat aggctttacc cggagcggca ttcagtcctt
    71461 ccttcactgg ttggacatcc aacacaccgg aaacaccatc taatgtgaca gttgcacgag
    71521 gagcgtaaat cagtcctaat ttggacccat catacaaatc aagtgtcccg gtaaatttta
    71581 tttctcccgt aaaatgatat ctgcttcccc aaaaatcggt catataagca atgataatgg
    71641 catttccatc gatatttggt gtcaatccac ctggatgtgc agctgttcct ttaaaattct
    71701 ggaactcgat cgactgaggt atctcattga tataaatatg gtgtgtgccg aaattgtgag
    71761 cgaatccacc aaaatcaata acgatatctc gtttgatctc taattccctg tctaaagtca
    71821 aaggctgaat cagtcggatc ttagttactg attcgtctgc gagaagctgc gccagttctt
    71881 ctgttgtggc agcttcgctg tacccatccg ctgaaacagc tcttgccagt gccgcttcac
    71941 tgctatcaga cgtttgttca gctacttctg aggtctcact tgtcttattt tcttcttgcc
    72001 ttttattagt agaagcctca ctttgagatt ctgtagtttc agaagtaatc gattcttctg
    72061 aaaaagtagt cttgtcaaaa ctttgattct ctgattgcgt tgattcgttt gttttctcta
    72121 catcggcaaa aacggatgtc accatcaaga gagggaagac aaacaaaatc gacgcaatca
    72181 gcttttttac acattgattc attctccacc atcctctctg acgttttgac aattttattt
    72241 tatcgtttta atctatttcc aattgtactt tcatatttaa aatatgtatt tttatggaaa
    72301 aatttttcta ttaggtgtaa atttcttttt tgtacaaaaa aaaggctgtg acaaaagtct
    72361 tgtcacagtc ccttatcccg aacaagcggt gttctcccgc caactcctcg gtcacaacct
    72421 ccattttaac aataaacctg taaatgtgag ggcaatactt tgattgtcac aggtaattta
    72481 tccccttcat ctccatcaac attcgtcgtc aattcttcct catcagctaa tgacacaact
    72541 aattgctcac aagtaaggta ggctagattt ttcgtagatt catctactcc ttttaatagg
    72601 tctggcaatg ttttaagcgt atcgatcaaa gaagaatctt ttaaataaac caaatggagc
    72661 ttaccatcat ccacttttgc atttggaagt aatgtttcaa aaccgccgac tgagttagtt
    72721 agtcctatca gcaaggtact gctctcaatt tcttccttct ttccatcaac ttctacgtga
    72781 aacgagtagg attgtgtgga agctaactgt ttgattcctg acatgaaata agctagtttt
    72841 ccaaatttcg tcttcttctc cgcatctaca tcattgattg attcagggat agagccaatt
    72901 gcgacgacat tcataaaata gtcgtcattt atttttccaa tatccaatgg cttgactgat
    72961 tcaatcgaaa aatgattgat cgcttcctca ggttctagcg gtatccctaa tgctcgagcc
    73021 aagtcattca ccgtacccaa tgggaaaaat ccgaaatttg gccgatgttc ttgttcagcg
    73081 attccactga ttccttcgtt gactgttcca tctccgccca tgacaaacac actatgatac
    73141 ccttccgtag ccgcttcgcg agtaaagttt tttgcatccc ctgctttttt tgtatgcaag
    73201 acgaccactt catcaaacac cgattccaat tttgctatcg ctaattgctc aaattccttt
    73261 gcttgttcac cgcctgaact ggggtttacc actaataaaa cttttttcat cactttcttt
    73321 cccttctgat tgatttcttt aatataacga tagcataaag aaaaaaatta ggctgtgatg
    73381 aagtactgtt catgaaacat tcatttattt tttgacaaga taaaacatcc atactatgaa
    73441 actgaggaaa attaaaacaa tgatatagga aataaaagaa aatgataggc attgtatacc
    73501 taataaagta aaaatcttag gtggcagata aaaacacaaa aatagcaaga gaatgtaata
    73561 gcgtttcttc cactttttgt tatcagacct tatagcgatc agaggaatac caaatgcata
    73621 actgaaaaac tcttgatgta ctgttaatat gtcacggcct tgaataataa atgcgacaat
    73681 ccccgctata attagtgtgc ctaacaccaa aacggaaaat tgatgaatat attttatata
    73741 caaatgatcc attgtacttc ctgccttttt atttgtgaga aaaacaaatc cgtatacgtg
    73801 aataacttct agaaataaaa gaaatttgca aaaccactcc atttatctac ttctacgtgc
    73861 attttttgat attgttttct ttatttaaga ttatacagaa aaatatctag ggtcgctgga
    73921 ttctaagtaa aaaaaatttt taaaataaaa agaactaaat caagtagtaa ttaaactact
    73981 gttgatttag ttctaataaa aagatttaga tgaacagttt tattgattta aatagttcat
    74041 attttttatt tataccgctt atttactact tgctgtacta gaatctgaag tgaatgtact
    74101 caaaacattt tcaaagtctt tgtctttgat ttttacatta gcgtctttca atacttcgcc
    74161 aattacttta ttctggaagg tactgtcact taattttgtg tctgttgcaa tttctttcaa
    74221 ttcttttttg tatttatcca tgtcgttgcc tttgttttgg tttttcacca ttttaaccac
    74281 ataatattcc gtagtataag ttgaagcatt ggtagaagtg atcacatcag aaacttgacc
    74341 atctttcaac ttaaaggctg cttctttgac ttctgccgga accgttgtag tagtggaatc
    74401 aaatttcact tttccgccat cttcttttgt cgtagtatca gttgacttgt ctttggctaa
    74461 tttagagaaa tcgtctccct tatcagcagc tttcttcaca tcttttgcat catcttcact
    74521 tgctacttgg ataatttgag cttccacttc tggatggaaa gattcccaag ccgtttttaa
    74581 atcttcatcg gtcaatttga tatgtttctt tagaccttct tgaaaggcta gactttgttt
    74641 gatactgtct ttgaatgttt gttctgtgta acctgcagat ttcaattgtg aatcaaatga
    74701 atcacccaat tgtttctttg tttgatcata ttgagaatcg atttctttgg ttgatacgtc
    74761 atctccatat ttatcttcaa atacttgata aacgatcaac tgttgcacag cggaatctgc
    74821 tccttgcatt tttttcaagc tatccattac ttcttgttcg ctgattgtac cgcctttata
    74881 actaacggct gttttttcac cggcattcgt acagcctgac aataagccag tgcctagtac
    74941 gatggcaagt gctaaaaatc tttttttcat ttatcaattc tcctactttt cgttttttgc
    75001 gaattgctaa aaacaaaggt gttcaagatg aaaatcatca agaacacctt tactaatctg
    75061 ctcgatacgt cctgaataga tataaggcta cacaatccag aaagttcgct cagcagaata
    75121 aatgtcaagt agcacatcac accttaatat actacttctt ttgttaccgt ttttcaagtc
    75181 tatcttgcgt tccgtattta acgtaactaa ttcttagaac aaatgttatc gattttgttt
    75241 ttcttcttcc gctgctggga tattcaaatc agacactttt atgttcgatt ttccagacat
    75301 ttttttgttg tttttattat gatatatttt tatacaacta aaaatgaata tattaaaact
    75361 accaaaaata acttttaacg ctgtttcatt atatgttttt tctattttat ataaactttt
    75421 ggatacttaa taccaaccgg ttggattgta ccaacatgag aatgtgtcag ttctatttcc
    75481 tgtcgcactt gcataggccc atactccttt tgttgcattt gctgatcgaa tagagtttcc
    75541 aactttcgct tttgcaccgt gacgattata gacacgacta tagtggttag aatgacctgt
    75601 ttttctcgta caagttgtat cactataata aacccatctt ccagagctat gattaattgc
    75661 tgttctagaa atttcccggt ctactggtaa atttgactta ataatattac ctttagcatc
    75721 aaaatgaatc tctcctggct ttaaagattt gtaatagatt tcaccgtgaa ctgaatttgc
    75781 ttccacttca gatgacatag cgtttcctcc aacaaaacct atccctagtg cacaaataat
    75841 aactgctttt ttcataaaac gacatccttt cctttttgat ttttaaagca tgatcttctt
    75901 gtttcctatt ttggtcagat atggatcatg cgtgactacg ataactaacg taccttggga
    75961 agcttgttgc gataagattt caactacttt atccttattt cttttatcca atgacccagt
    76021 cggttcatcg gctaaaatga ttttgctatt cttcaataag actctcgcac aggcgattct
    76081 ttgcttctca ccaccagata atgtgtagat aggcgtactt agcttcttgt ttatatttac
    76141 ttttttcaat gcctcatact ttgctgttcg cttatctgaa acatttaaat tcgaaaactt
    76201 caatccaata tctaaattaa aatcgattgt ttcatattca atcaacccaa aatcttgaaa
    76261 aagaaaagat atggtatttc tttttatcaa gcgcttctct ctcccagtta attgtttcgt
    76321 ctcttgatcg aacaaataga attgtcctga actgaactga tcattcaatc ctaatatatt
    76381 caataacgta cttttacctg ctccactttc tccaacaaca gtcacaagat ccccagcatc
    76441 cgcagtgaaa cgatccaaat gaagaatcgt tttgtttcga atgatttttt tcgcaccttt
    76501 tatcactatc aacggctcca attgttaacc tccattcatc agcaataact gacttttctt
    76561 ttctgtagac aaaataaaaa caactgaaag aataaaatca atgaagctaa agaaaaaaat
    76621 attaaaaaga tatttaggct ctctttctat aagaaaaagg aagattacac ttgtcgttcc
    76681 ccatttgatg aggttcatca tgaagaaatc tttatacgtt tcaaagagcg aataaccact
    76741 tgaacgaaag atagcgattt tccgacaatt gtagacaaaa tatatgcata acatgacata
    76801 gctcaacaag aagaaagaaa agaaagtgag taaaaatatc tgtatcaagt aatgaaatga
    76861 tctgcttaac actttgacag ctatctcttc agtctgagta tacggaaatg ataactgcaa
    76921 gttatcacgt aactgacttt cttcaatcag ctcaaataac ctttctttcg acaaagagcc
    76981 tatcttgatc tttaacggtg gatagatttc tccgctgaga atatttcttt ccaaatacat
    77041 actgtttttc aatgtcagta tttcaacaac cggataatgt tcgatccagg ggatagaagg
    77101 aataaatgta taaatgggtt gattgttttt tataaataga aatttcggtt ctttttcact
    77161 gatttcttga taatattctg ttatttgcgc caggtcattt tttaaatctg tatgtctata
    77221 tcgttcaggg atcaatataa cagggtgcgt ttctttttcg gttatttgga tcaaccttcc
    77281 agagacatca atcaaaggaa atttcttaag ataattcaga tttattttta tccctctgcc
    77341 aaactgttca ttttcttcat tcaagtaatc tgatacattg actaatatac ttccgttttt
    77401 attcaaagtt tcatataatt cctgatctgc tgctgatgca aaacgattgg aataaataaa
    77461 atccacgtgg tttttcccga tggttactgg ataaaataca tgatagtcct caccagcgat
    77521 ttcttttggt ggttcatggg aaacagctaa ctgtgaagaa gaaaaaacga tagttgctaa
    77581 ttcgacattg gcaacggtca aaagaagaga aaacaacaat ccaaatgtaa aaagaatgta
    77641 ttggatatag ttgaacgctt ctcttaacga agcgattttt tcaactaata aaagagaaat
    77701 caaagagata ccatatattc cgctgaacaa gaaaacgtac atctggaaca aataaagaaa
    77761 ttgctcctta taatacataa aaataaaacc accaatgatt cctgcaatta tgaatgtaat
    77821 aatgattttc tttttcccca ataaacacca caatacttga tgtaatgaaa agtcattatt
    77881 caacaataaa ctgatttctt tattcatcaa taagaaaaag gatgataaca aaaggaataa
    77941 aagaatcaat ccaatagtta aatacactcg ataattatat acgtctgtat ttgaaataac
    78001 cgaatacgaa tagctatttt caaagtcttc aaaaatatta gtggcataat cagtgtgaaa
    78061 tgcttgattg aatcgatcag tgaatagtga aatgaaccgt tcataggctt ctttttctct
    78121 cgtcatcaag aaaaattcac cttcaaaatc ttttattttg atcgattctt caatggacgt
    78181 tacgcgaaag gaggtattag aatagggtac cttctcgtga gaagatttga catccccttg
    78241 ataatacaat gtcatttcaa gctttggctg tatcttgaag ttccaagatg tatctacttc
    78301 attggattca tttactgttt ttttcatata agaaagctca gtttctgatg agacattttc
    78361 aaataccatg attagctgct catattcttc ttgcccaata aaatgttgtg gcaatcgaat
    78421 cggagtataa gtatctgttt gatggaaaaa ttcatttgtc cgatagagta cctgtttatt
    78481 tatactaacc aaggcaaaac cactcatgat accggtcact atgattgtga gtaaaactgt
    78541 gaactttttt ttgattatca ccactctccc ttacttaaat gattctttga taaaatcata
    78601 ccgtttttca tcaaaaaaac gtatctatca agtgtatagg tcatgtagaa taaatctata
    78661 tactgtagaa tcgaaaggag aagaagatga acaccggaga aaaaatacga acttaccgca
    78721 gaaattgtaa gcttacgcaa aaagaattag gcgaaaaaat tggtgtttcc gataaaacga
    78781 tttctagttg ggaaaattct cgtacaatgc cagaccttga aatgctgtca cttttacatc
    78841 atactttagg tctgccgatt gattttcctt ctattgaatc agctaccgaa tcaatagaac
    78901 caacattgcc agatgaacct cttgttaaca agaaatctta ttcatcaaaa caacccttct
    78961 cactcaaaaa gacactatct ttctttctgc tcgtcttttg tttggtctat ctttttctta
    79021 tcaattatca atacaaaaat gaaacgatcg atcgattcaa tccttttttg aaaccacaaa
    79081 tcggctatac cacactccct gtaggatacg tggacaattc catcgaacgt tgggtatgta
    79141 atgatatctt tggccatggt acatggctgt cttttcacgc tggagaggta gaaaaagaga
    79201 aaaaccatgc tgtaatcaaa cacaaaggaa aacacgtcat gaacgttcgc ctgctcactt
    79261 gggaagccat tccctcagat taccaagaaa tcctcagcca agactatgac gacgccttaa
    79321 aaagaagccc tcaggagcca gataggcatt aagtaggaga tgaacgtaga gatagaaaaa
    79381 atcaatgcac atgatcttat ccaaaaaaca ggggtacaaa ttaattgaaa aagccttgag
    79441 atgaaagttc ctaaataaaa aagttcagaa tgataggtat ttctggctta tagtataata
    79501 tttacgccac agcctcccaa tgattctttt tggaatcata atcaaggatg gacattcaca
    79561 aaaatatttt gctcattcga tccgaataga ttaacgacca atctagtaag taatatagat
    79621 tggtacgtag tcaagctgca cataatttag atcaatttta gttctttttt atatttctat
    79681 gatctgctca tttactttta tatgatggct tacagtaaca taaataaata tgataatcgc
    79741 tggtagttta tccaagagta ctcaataaaa tacaaaatgg attgttcaag cgcctatcat
    79801 aatgataaaa tgaaacaaat aaacgataca aggaggaaga gactttgagt aatggcagaa
    79861 caattttttc attgtaaaaa ttgttcagtt gttaatctat gggatttcgg tagcatgggc
    79921 atttttttat agaggaatca caccaacctg gccgttttat ctcttgttag tgggaatatt
    79981 agtaggaaat tttgcccctc ttcactatcg tacgaacttt tttccaactg attctggcct
    80041 ttttcttaaa acaaagacgc gaaaattaga aaacattatt gaaatgacaa ttacctgtat
    80101 aatcattttc ttagttatat tcctaaacgt cttctaataa gaatctctta aaattagctt
    80161 gttacagtta gttacctagt ttatataaca cagttatctt atcttcctta tcaacatctt
    80221 ttgccaactc tttgtcttaa attcgaaaca tataagcaga gctacgaaca ccaagactgt
    80281 ttcggctctg ttttttgcat caaattcact cttatatagc tgataatctc ggctctttaa
    80341 aagaaattat tttcgcttca ttttattata aaaaggctag gaaccttgat gttatcaggt
    80401 tcctagcctt tatttaagta ttaccccaca gacccttcca tatagatttt taaattacac
    80461 actagtttaa actaaaacaa acaaggaaac acttaccatt taatcataaa attagctcat
    80521 atgaatatat tagaatgtaa aatagactgg tcaaaatctg gtcaaaaaag aggactaata
    80581 caggtagt
//
LOCUS       DACCYH010000002        79287 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.002, whole genome shotgun sequence.
ACCESSION   DACCYH010000002 DACCYH010000000
VERSION     DACCYH010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 79287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.002"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            405..3239
                     /locus_tag="IVY85_000073"
     CDS             405..3239
                     /locus_tag="IVY85_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8043119.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            3430..3921
                     /locus_tag="IVY85_000074"
     CDS             3430..3921
                     /locus_tag="IVY85_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP8043120.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            4024..5013
                     /gene="manX"
                     /locus_tag="IVY85_000075"
     CDS             4024..5013
                     /gene="manX"
                     /locus_tag="IVY85_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP8043121.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            5046..5849
                     /locus_tag="IVY85_000076"
     CDS             5046..5849
                     /locus_tag="IVY85_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP8043122.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5870..6784
                     /locus_tag="IVY85_000077"
     CDS             5870..6784
                     /locus_tag="IVY85_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP8043123.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6867..7241
                     /locus_tag="IVY85_000078"
     CDS             6867..7241
                     /locus_tag="IVY85_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP8043124.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7380..7625
                     /locus_tag="IVY85_000079"
     CDS             7380..7625
                     /locus_tag="IVY85_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8043125.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            7797..8861
                     /locus_tag="IVY85_000080"
     CDS             7797..8861
                     /locus_tag="IVY85_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8043126.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            9107..9532
                     /locus_tag="IVY85_000081"
     CDS             9107..9532
                     /locus_tag="IVY85_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8043127.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            9525..9974
                     /gene="nusB"
                     /locus_tag="IVY85_000082"
     CDS             9525..9974
                     /gene="nusB"
                     /locus_tag="IVY85_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP8043128.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            10071..10925
                     /locus_tag="IVY85_000083"
     CDS             10071..10925
                     /locus_tag="IVY85_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP8043129.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            10926..12263
                     /locus_tag="IVY85_000084"
     CDS             10926..12263
                     /locus_tag="IVY85_000084"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP8043130.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12268..12492
                     /locus_tag="IVY85_000085"
     CDS             12268..12492
                     /locus_tag="IVY85_000085"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP8043131.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            12494..13384
                     /locus_tag="IVY85_000086"
     CDS             12494..13384
                     /locus_tag="IVY85_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8043132.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            13393..14211
                     /locus_tag="IVY85_000087"
     CDS             13393..14211
                     /locus_tag="IVY85_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP8043133.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            14318..14767
                     /locus_tag="IVY85_000088"
     CDS             14318..14767
                     /locus_tag="IVY85_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8043134.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            14785..16467
                     /gene="recN"
                     /locus_tag="IVY85_000089"
     CDS             14785..16467
                     /gene="recN"
                     /locus_tag="IVY85_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP8043135.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(16522..16638)
                     /locus_tag="IVY85_000090"
     CDS             complement(16522..16638)
                     /locus_tag="IVY85_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP8043136.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(16711..17667)
                     /locus_tag="IVY85_000091"
     CDS             complement(16711..17667)
                     /locus_tag="IVY85_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8043137.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            17838..18206
                     /locus_tag="IVY85_000092"
     CDS             17838..18206
                     /locus_tag="IVY85_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP8043138.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            18376..18807
                     /gene="mraZ"
                     /locus_tag="IVY85_000093"
     CDS             18376..18807
                     /gene="mraZ"
                     /locus_tag="IVY85_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP8043139.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            18828..19787
                     /gene="rsmH"
                     /locus_tag="IVY85_000094"
     CDS             18828..19787
                     /gene="rsmH"
                     /locus_tag="IVY85_000094"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP8043140.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            19792..20193
                     /gene="ftsL"
                     /locus_tag="IVY85_000095"
     CDS             19792..20193
                     /gene="ftsL"
                     /locus_tag="IVY85_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP8043141.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            20194..22386
                     /locus_tag="IVY85_000096"
     CDS             20194..22386
                     /locus_tag="IVY85_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8043142.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            22412..23374
                     /locus_tag="IVY85_000097"
     CDS             22412..23374
                     /locus_tag="IVY85_000097"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP8043143.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            23388..24749
                     /locus_tag="IVY85_000098"
     CDS             23388..24749
                     /locus_tag="IVY85_000098"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP8043144.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            24775..25863
                     /gene="murG"
                     /locus_tag="IVY85_000099"
     CDS             24775..25863
                     /gene="murG"
                     /locus_tag="IVY85_000099"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP8043145.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            25878..27098
                     /locus_tag="IVY85_000100"
     CDS             25878..27098
                     /locus_tag="IVY85_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP8043146.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            27254..28579
                     /gene="ftsA"
                     /locus_tag="IVY85_000101"
     CDS             27254..28579
                     /gene="ftsA"
                     /locus_tag="IVY85_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP8043147.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            28602..29843
                     /gene="ftsZ"
                     /locus_tag="IVY85_000102"
     CDS             28602..29843
                     /gene="ftsZ"
                     /locus_tag="IVY85_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP8043148.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            29860..30537
                     /locus_tag="IVY85_000103"
     CDS             29860..30537
                     /locus_tag="IVY85_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP8043149.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            30551..31150
                     /locus_tag="IVY85_000104"
     CDS             30551..31150
                     /locus_tag="IVY85_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP8043150.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            31165..31452
                     /locus_tag="IVY85_000105"
     CDS             31165..31452
                     /locus_tag="IVY85_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP8043151.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            31501..32283
                     /locus_tag="IVY85_000106"
     CDS             31501..32283
                     /locus_tag="IVY85_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP8043152.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            32304..33011
                     /locus_tag="IVY85_000107"
     CDS             32304..33011
                     /locus_tag="IVY85_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP8043153.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            33273..36071
                     /gene="ileS"
                     /locus_tag="IVY85_000108"
     CDS             33273..36071
                     /gene="ileS"
                     /locus_tag="IVY85_000108"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP8043154.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(36120..37646)
                     /locus_tag="IVY85_000109"
     CDS             complement(36120..37646)
                     /locus_tag="IVY85_000109"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP8043155.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(37661..38308)
                     /locus_tag="IVY85_000110"
     CDS             complement(37661..38308)
                     /locus_tag="IVY85_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP8043156.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(38492..38821)
                     /locus_tag="IVY85_000111"
     CDS             complement(38492..38821)
                     /locus_tag="IVY85_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP8043157.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            38998..39726
                     /gene="sfsA"
                     /locus_tag="IVY85_000112"
     CDS             38998..39726
                     /gene="sfsA"
                     /locus_tag="IVY85_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP8043158.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            39742..40755
                     /locus_tag="IVY85_000113"
     CDS             39742..40755
                     /locus_tag="IVY85_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8043159.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            40755..42032
                     /locus_tag="IVY85_000114"
     CDS             40755..42032
                     /locus_tag="IVY85_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8043160.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            42095..44797
                     /locus_tag="IVY85_000115"
     CDS             42095..44797
                     /locus_tag="IVY85_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8043161.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            44949..45266
                     /locus_tag="IVY85_000116"
     CDS             44949..45266
                     /locus_tag="IVY85_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8043162.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            45296..45616
                     /locus_tag="IVY85_000117"
     CDS             45296..45616
                     /locus_tag="IVY85_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8043163.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            45724..47184
                     /locus_tag="IVY85_000118"
     CDS             45724..47184
                     /locus_tag="IVY85_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8043164.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            47252..47473
                     /locus_tag="IVY85_000119"
     CDS             47252..47473
                     /locus_tag="IVY85_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043165.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            47504..47686
                     /locus_tag="IVY85_000120"
     CDS             47504..47686
                     /locus_tag="IVY85_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP8043166.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            47686..48099
                     /locus_tag="IVY85_000121"
     CDS             47686..48099
                     /locus_tag="IVY85_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP8043167.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            48222..49403
                     /locus_tag="IVY85_000122"
     CDS             48222..49403
                     /locus_tag="IVY85_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8043168.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            49792..49865
                     /locus_tag="IVY85_000123"
     tRNA            49792..49865
                     /locus_tag="IVY85_000123"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49826..49828,aa:Arg,seq:cct)
     gene            complement(49934..51073)
                     /locus_tag="IVY85_000124"
     CDS             complement(49934..51073)
                     /locus_tag="IVY85_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8043169.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(51372..52007)
                     /locus_tag="IVY85_000125"
     CDS             complement(51372..52007)
                     /locus_tag="IVY85_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043170.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(52120..52755)
                     /locus_tag="IVY85_000126"
     CDS             complement(52120..52755)
                     /locus_tag="IVY85_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAP8043171.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(52789..53250)
                     /locus_tag="IVY85_000127"
     CDS             complement(52789..53250)
                     /locus_tag="IVY85_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAP8043172.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(53380..53811)
                     /locus_tag="IVY85_000128"
     CDS             complement(53380..53811)
                     /locus_tag="IVY85_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8043173.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(53829..54149)
                     /locus_tag="IVY85_000129"
     CDS             complement(53829..54149)
                     /locus_tag="IVY85_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8043174.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            54448..55224
                     /locus_tag="IVY85_000130"
     CDS             54448..55224
                     /locus_tag="IVY85_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP8043175.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            55239..55442
                     /locus_tag="IVY85_000131"
     CDS             55239..55442
                     /locus_tag="IVY85_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8043176.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            55458..55796
                     /locus_tag="IVY85_000132"
     CDS             55458..55796
                     /locus_tag="IVY85_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP8043177.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            55783..55962
                     /locus_tag="IVY85_000133"
     CDS             55783..55962
                     /locus_tag="IVY85_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043178.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(56005..56475)
                     /locus_tag="IVY85_000134"
     CDS             complement(56005..56475)
                     /locus_tag="IVY85_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043179.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            56562..57260
                     /locus_tag="IVY85_000135"
     CDS             56562..57260
                     /locus_tag="IVY85_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP8043180.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            57438..57779
                     /locus_tag="IVY85_000136"
     CDS             57438..57779
                     /locus_tag="IVY85_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043181.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            57772..58443
                     /locus_tag="IVY85_000137"
     CDS             57772..58443
                     /locus_tag="IVY85_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP8043182.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            58449..59141
                     /locus_tag="IVY85_000138"
     CDS             58449..59141
                     /locus_tag="IVY85_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043183.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            59138..59887
                     /locus_tag="IVY85_000139"
     CDS             59138..59887
                     /locus_tag="IVY85_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8043184.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            59899..60168
                     /locus_tag="IVY85_000140"
     CDS             59899..60168
                     /locus_tag="IVY85_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043185.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            60331..60630
                     /locus_tag="IVY85_000141"
     CDS             60331..60630
                     /locus_tag="IVY85_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043186.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            60630..60932
                     /locus_tag="IVY85_000142"
     CDS             60630..60932
                     /locus_tag="IVY85_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043187.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            60972..61337
                     /locus_tag="IVY85_000143"
     CDS             60972..61337
                     /locus_tag="IVY85_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043188.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            61964..62146
                     /locus_tag="IVY85_000144"
     CDS             61964..62146
                     /locus_tag="IVY85_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043189.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            62159..62353
                     /locus_tag="IVY85_000145"
     CDS             62159..62353
                     /locus_tag="IVY85_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043190.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(62386..62598)
                     /locus_tag="IVY85_000146"
     CDS             complement(62386..62598)
                     /locus_tag="IVY85_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043191.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            62723..62926
                     /locus_tag="IVY85_000147"
     CDS             62723..62926
                     /locus_tag="IVY85_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043192.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            62923..63195
                     /locus_tag="IVY85_000148"
     CDS             62923..63195
                     /locus_tag="IVY85_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HAP8043193.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            63196..63381
                     /locus_tag="IVY85_000149"
     CDS             63196..63381
                     /locus_tag="IVY85_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043194.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            63368..63715
                     /locus_tag="IVY85_000150"
     CDS             63368..63715
                     /locus_tag="IVY85_000150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043195.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            63675..64151
                     /locus_tag="IVY85_000151"
     CDS             63675..64151
                     /locus_tag="IVY85_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HAP8043196.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(64297..65025)
                     /locus_tag="IVY85_000152"
     CDS             complement(64297..65025)
                     /locus_tag="IVY85_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HAP8043197.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            65073..65351
                     /locus_tag="IVY85_000153"
     CDS             65073..65351
                     /locus_tag="IVY85_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043198.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            65353..65739
                     /locus_tag="IVY85_000154"
     CDS             65353..65739
                     /locus_tag="IVY85_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043199.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            65736..66116
                     /locus_tag="IVY85_000155"
     CDS             65736..66116
                     /locus_tag="IVY85_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8043200.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            66228..66680
                     /locus_tag="IVY85_000156"
     CDS             66228..66680
                     /locus_tag="IVY85_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HAP8043201.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            66677..68404
                     /locus_tag="IVY85_000157"
     CDS             66677..68404
                     /locus_tag="IVY85_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8043202.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            68468..69655
                     /locus_tag="IVY85_000158"
     CDS             68468..69655
                     /locus_tag="IVY85_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8043203.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            69621..70196
                     /locus_tag="IVY85_000159"
     CDS             69621..70196
                     /locus_tag="IVY85_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAP8043204.1"
                     /translation="MEMKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWD
                     DLSEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDT
                     TVARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYED
                     TEAALRSRDELEKNVEERKKLIKKINQALEA"
     gene            70209..71573
                     /locus_tag="IVY85_000160"
     CDS             70209..71573
                     /locus_tag="IVY85_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8043205.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            71575..71853
                     /locus_tag="IVY85_000161"
     CDS             71575..71853
                     /locus_tag="IVY85_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP8043206.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            71834..72160
                     /locus_tag="IVY85_000162"
     CDS             71834..72160
                     /locus_tag="IVY85_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8043207.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            72150..72488
                     /locus_tag="IVY85_000163"
     CDS             72150..72488
                     /locus_tag="IVY85_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043208.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            72478..72843
                     /locus_tag="IVY85_000164"
     CDS             72478..72843
                     /locus_tag="IVY85_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP8043209.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            72850..73476
                     /locus_tag="IVY85_000165"
     CDS             72850..73476
                     /locus_tag="IVY85_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8043210.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            73476..73940
                     /locus_tag="IVY85_000166"
     CDS             73476..73940
                     /locus_tag="IVY85_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043211.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            74135..76444
                     /locus_tag="IVY85_000167"
     CDS             74135..76444
                     /locus_tag="IVY85_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8043212.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            76456..77160
                     /locus_tag="IVY85_000168"
     CDS             76456..77160
                     /locus_tag="IVY85_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8043213.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            77157..>79287
                     /locus_tag="IVY85_000169"
     CDS             77157..>79287
                     /locus_tag="IVY85_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043214.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWM"
BASE COUNT        26863 a        13030 c        16647 g        22747 t
ORIGIN      
        1 gaatcttatg attaaagagg ttgtgacaag acttttgtca cagccttttt tgtttataaa
       61 aataaaagtt gtagtggagg ctacgaagtt tactttgtct ttttacactt aatatttttt
      121 tgccaatttg gaatcaggat gttttgataa ctatcgtttt caattgatgg aagctatatt
      181 gtttgctatc agtgaaataa ttatatggta aacagtgttc tttttcttgt ttccattata
      241 aaaatcgtat cgggattttt cgttctttta aattgaacat ttctatctat ccgcgtgtta
      301 tcaacaaaac aaaatcaggt aaatagttga cgaaacagaa ataaatacat agtattatgt
      361 tttgaagcct aagtacaata gtctatagaa aaggagcgat accattggca aaacgcattg
      421 accgaattta tatgtatgtc aaagaaaaaa ccgctcattt aacaaaggca gaatatgatc
      481 aaggagtaac aacacaggaa attgctgaag tgttagggat tcagcgaaca aatagcagta
      541 aagatctgaa tcagctagtg cgcgaaggaa aactgataaa gacagacgga cgaccagtac
      601 gttacatata tcaaacactc gttacgcacg taaagccagt tacaaaacat gtagtcagtt
      661 acaaagaaac gccggtctct gaagaaaaaa atacactcca aatagatact aaggatatct
      721 ttgcaaaaat cattggagct aacggcagca tgaaaaattc agtggaacag gcaaaagctg
      781 cgattttgta tccgccaaga ggattaaatt gtttgattac aggacctacc ggatcaggaa
      841 agacttattt tgcacacgcg atgtatcatt ttgcaaaagc gcatcatgta atagatgagg
      901 aaacggaact gattgttttc aattgtgctg attatgccaa taaccctgag cttttgatga
      961 gtcatttatt tgggtacgca aaaggtgctt ttacaggcgc agatgaagaa aaaatgggga
     1021 ttatcgatca agcggatggc ggtatgctgt ttttggatga gattcaccgg ttgccgcctg
     1081 aaggtcaaga aatgatcttt tactttatgg atcatggaac ttacagtcgt cttggggaaa
     1141 caacgaaatc ccatgaagca aatgtacgga tcgtaggtgc gaccacagaa gacccaggtt
     1201 cttctctatt agaaactttt gttcggcgta tcccaatcaa tatcaaactt ccggcttttg
     1261 aaaaacgtcc tgcaaatgag aaagtcgatt tagtaaaaat catgattgcc cacgaagcaa
     1321 accgcacgca gcgaaagatt tctttgacag aagatgtagc aaaagcattg atcggcagtg
     1381 tgacctatgg aaatatagga caattgaagt caaatatcca gttagtatgt gcacgaggat
     1441 ttctaaatca tatgcaatca cccgaaatct cgatcacaat cgatgatttg acagaaggga
     1501 ttcgtagcgg attgattcaa ttagcaagca accgcagtgc aatggcagag ctttctaaaa
     1561 tattggagcc aaagataact gtagcaccaa atgatacgat catgaaaatc caatcggact
     1621 cctatgagtt accttataat ctatatgata tcatcggaga taaagcagct ttactgaagt
     1681 cggatggttt ggatcaagaa gcaatcaatc atttcatttc tacagatatc aacatccatc
     1741 tgaagtcttt ctataaagac catggttttt cttttaatgc agacagcaaa ttagcagaat
     1801 ttgtagatcc gaaaatcata gaagtgacca atcagattta tgtaatggtc aaaagatctt
     1861 taccatatga gtttcagcaa aactttattt atgctatgag cttgcatatc agttcttttt
     1921 tgaaaaaaat caacttagga gaagaacggc atacgaatga taacatccgt gagatggctt
     1981 ttgattttcc agatgaatat gctgttgcga aagaagtgcg tagttatatc gaaacgtatt
     2041 ttcaagtgga gattccagaa agtgaagact attacttgac cgttctactc gtctcgttac
     2101 gggcaaatca agcaagcggc agaatcggtg tagtcgttgc agctcacgga aacagcacag
     2161 caagcagtat ggtccaagtt gtccaacagt tgttagatgc agagaacgta cgagcagtag
     2221 acatgccttt ggatatggat ccgaaaacag cattggaccg aattgaacgc aatgtacaag
     2281 aagtagatga aggaagcggg gtcatactct tagtagatat ggggtccctt gcgtcgttta
     2341 attcgcaaat tcaaagagac actggaattc cagtacgtac tgttgatatg gtaacaactt
     2401 ctctagtttt ggaaactgta cgaaaggtct cagtattagg gacagatcta gatatgttgc
     2461 atgaatcatt gaaaaatttt cgaggttacg cagaaatatc ttctgtagaa tctgttcagg
     2521 aagcaaaacc tattcataag aaaaaagctg ttttggctat ctgcgcatct ggcgaaggta
     2581 cagcacaaag aatcaaagaa ttgatcgaac gagcggtaaa caagcgtcaa gaaacagaat
     2641 tagaagttct agctttgtcg attgtggaag tgaaagaaga actgccagtt atccaaaaag
     2701 aatatcagat catagctact actgggatta ctgatttgaa tatccaagcc ccatttattc
     2761 ctttagaacg ttttattgat caaaatatcg aagtcatttt ggatcagttg ctgatggaat
     2821 cagaattgga ggaaactgaa ttcatctccc tagatgaaga gtcagctaaa aatacgtgtg
     2881 ttgagtttat ctctgataac ttcatcttta ttaatggtag taagttaatc gatccaatgt
     2941 ggcaattttc tacacaaatc tctcagacga cgggaatcgg cgatgaagaa tatggattca
     3001 aaatcaacct agtgatgcac acagcaggaa tgatcgaacg tattattcgg aatgaaccat
     3061 tgactgtgga agaaaacgaa ttgacaaata caacgaatga tcctctttat agccaattgg
     3121 cagcctcagt tgttctgttg gaagatcaga taaaagtaaa agtgccgata gaagaaatgt
     3181 attatctgct acgtctagtc cataatcagt tagataaaaa agaatacact gtgccatgaa
     3241 tacacaaaaa cgatacactt ttatagtgta tcgtttttgt gtattggtta agaatccttt
     3301 aaattcaatt agtgtatcgt aaaaaagttg gcacgctttt tgcttatagt agtgtgtgct
     3361 aggaggtggt tgttcacaca gaaatgatag gggtttctgt gaagtagtga ggaagaagga
     3421 gttttgcaga tggatattcg attggcacgt atagatgatc gtttaatcca cgggcaagtc
     3481 gcaacagttt ggtcgaagca aacaggtgtt gaacgtatca ttgtgatcag cgatgtagtc
     3541 gctcaagata aattgagaaa gtttctacta aaagaagctg caccgccggg gatcacagca
     3601 aatgtaataa ccgtgcagaa aatgattcaa atctatcata gtggattatt atcacgagca
     3661 aaagtgatgc ttttattcac caatccccaa gatgttgaag cggttgtccg ctgcggtgta
     3721 cagttgaagt cgttgaatat cggcggtatg agttttactg aaggcaaatc aatgattacc
     3781 aattttgttt cagtaaatca acaagatatt gattcctttc gttacttgaa cagtcaagaa
     3841 gtggagttag agattcgaaa agtacctgct gacaggaaaa tccttttgat ggaactacta
     3901 gcgaaaatca aaaaggtgta gcaagaatgg ttgttggaaa aggtcaaata attctgttgt
     3961 aatggttagc aattattaat atttttgaat ttgttacaac aaaagtaaat aggaggtata
     4021 aatatggtag ggattatcct agcaagtcat ggacaatttg ctgaaggaat cttacaatcc
     4081 ggttcaatga tctttggcga acaagaaaac gtcaaagccg tgattttgaa accaagtgaa
     4141 ggtccagatg acttgagagc caaattggaa gaagcggttg cttcttttga taatcaagac
     4201 gaagtgttat tcttagttga tttatggggc ggaacaccgt tcaatcaatc aaacacatta
     4261 ttcgaagagc acaaagataa atgggcaatc gtcagcggtt tgaatttacc aatgttgatt
     4321 gaagcctacg catcacgctt ttcaatggaa tctgctcatg aaattgcagc acacatcatt
     4381 gaaacagcaa aagacggtgt aaaagtaaaa ccggaagaat tagaaccggc agaagcaccg
     4441 aaagcagcag ttgaagacgc acaacctaaa ggagcacttc cagaaggaac tgtggtcgga
     4501 gacggaaaaa tcaaatatgt attagcacgt gttgactcac gtctgttaca tggacaagtt
     4561 gcgacagctt ggacaaaagc agtgcaacca aaccgtatta tcgttgtttc agatgcagta
     4621 tcaaaggatg atctgcgtaa gagactaatc gaacaagctg ctcctccagg tgtaaaagca
     4681 aatgttattc ctattagcaa aatgatcgaa gtagcaaaag accctcgttt tggaaatacc
     4741 aaagcattgc ttttatttga aaatcctgaa gacgtattga cagctgttga aggtggcgta
     4801 gacatcaaag aattaaatgt tgggtcaatg gcgcattcgg tcggtaaagt cgttgtaagt
     4861 aaagtattgt ctatgggaca agaagacgtt gaagcgtttg agaagcttga acaaaaaggt
     4921 gtgaaattcg atgtacgtaa agtacctaat gattcacgcg ataacatgga tgatattttg
     4981 aaaaaagcaa aagctgaatt agcaaaagcg taataaataa tattaaaatt tataggaggc
     5041 ttatcatgtc tattatttca ataattttag tcgtattagt tgcctttcta gctggtatgg
     5101 aaggtattct tgatgaattt caatttcatc aaccactagt ggcatgtaca ttgatcggtt
     5161 tagttaccgg taacctcgaa gctggtatcg tacttggcgg tacgcttcag atgatcgctt
     5221 taggttgggc aaatatcggt gccgctgtag caccagatgc tgcattagca tcagtagcat
     5281 cagcaattat tttagtttta ggtggacaag gtgttaaagg tgttccttct gcgatcgcga
     5341 ttgccgttcc tttggctgtt gcagggttgt tcttaacaat gatcgtacgt acaatcgctg
     5401 ttccaatcgt tcatttgatg gatgcggctg ctgaagaagg aaacatccgt aaggttgaaa
     5461 tgtggcacat cattgctgta tgtttacaag gggttcgtat cgcggtccca gcagcagcgt
     5521 tgctatttat cccagcacaa actgtgcaat cattccttga atcaatgcca gcatggttaa
     5581 cagatggtat ggctatcggt gggggaatgg tcgtagctgt aggttacgct ttggtaatca
     5641 acatgatggc tacaaaagaa gtatggccat tcttcgttat tggtttcgtt gtagctgcaa
     5701 tttctcaatt aacattgatt gctctaggtg ctttaggtgt tgcattagct cttatttact
     5761 tgaacctttc taaaatgggt ggttcatcaa ataatggagg cggaagcaac tctggtgacc
     5821 ctttaggcga cattttgaat gactattaat cttgaaggag gaaaataaaa tggcagaaga
     5881 aaaaatcaaa ttatcaaaaa aagatcgttt agctgttgcg tggcgctcta ccttcattca
     5941 aggttcatgg aactacgaac gtatgcaaaa cggtggctgg gcattttcta tgattccagc
     6001 aatcaaaaaa ttatacaaaa caaaagaaga acgttctgca gcattaaaac gtcacttgga
     6061 attctttaat acacacccat acattgcttc tccaatttta ggggttacat tggctctgga
     6121 agaagaacgt gcgaatggcg cgcctgttga tgatgtagcg attcaagggg ttaaagtagg
     6181 tatgatgggt cctttagctg gtgttggtga cccagtgttc tggtttacag tacgtccaat
     6241 gctaggtgca ctaggtgctt cattagctat gggaggaaat atccttggac caatcatctt
     6301 cttcttaggt tggaacatta ttcgttgggc attcatgtgg tatacacaag aatttggcta
     6361 caaagctggt tctaaaatta ctgatgacct ttctggcgga ttgttacaag acgtaacaaa
     6421 aggtgcttca attttaggga tgttcgtatt agctgccctt gtacaaagat gggtatcaat
     6481 caaattctta ccagttgtat caactgtaaa attagataaa ggtgcttaca tcgaatggga
     6541 taaattgcct tcaggtggtg aaggaatcaa gagtgcattt gaacaagtta ataatggctt
     6601 agccctttct cctgaaaagg taacaacatt acaagacaac ttagaccaat tgattcctgg
     6661 tttagctgca ttaggattaa cattgttatg tatgtggttg cttaagaaaa aggtttctcc
     6721 aatcgttatc atccttggtt tattcgtagt cggtgttgtt ggacacgtca tcggactttt
     6781 ataatccata aaaatatagc tcgggcgttg tcccgagctt tttttatata gtatactgtt
     6841 cataaacaaa tatctggagg gagaaaatgg ttcaatcaat caatacaaaa gtagacttgg
     6901 taattgatgc tacttcgttt acgggtctga cagattacgg gcaaatcatg ataggtaata
     6961 atggttttga gttttaccat gcgcgagata gacgcaaata tatccaaatt ccatgggaag
     7021 aagtggatta tgtactagca tcagtcatgc taaagggaaa atggattcct cgatatgcca
     7081 ttcaaacgaa aaaaaatggt acatacacat tttcttccaa agaacctaaa aaagtgcttc
     7141 gtgcgatcag agaatatgta gatgaagata gaatggtaca atcattgagt ttctttgatg
     7201 tagtgaaacg tggtattcgt agtatgttta aacgcaagta aaaaataaag aaaacttcta
     7261 agaggaggtt ttctttattt ttttactttg cagaagaaaa tatgaaatag caaaaccaat
     7321 tatttgtccc ctttaaatcc catatgctaa aattgattag caaagaagga ggttatatta
     7381 tgagttggtt atggacattg attgttggag ccgtgatcgg agcaattgca ggtgcgatca
     7441 ccagtaaagg aaattcgatg ggctgtgtaa cgaatatcat tgctggacta gtcggttcaa
     7501 gtattggaca agcgattttc ggcgaatggg gaccaaaagc tgcaggtatg gcacttattc
     7561 catctatttt aggcgcagtg attttggtag cagttgtctc atttttcttt ggaaaacgat
     7621 cataagtttt tacaaaatag taaactaaaa ggagttgcag cgagactgtg actccttttt
     7681 ggctttattt atttaaaata aaaagaagtt ttccggaaat ttttatttaa ctgtcgtgta
     7741 cgaagaaaag tttgctataa tggaaaaaag cgtgcaaaga aagagggagt ttcattatga
     7801 tgctacgtgt agaaaaattg cgcaaaaaaa tgcaagaaga aaatttggat tcatttttgg
     7861 ttaccagtcc gtacaatctt cgttacttaa cgaactttac aggtacgaca ggattagctg
     7921 ttattacttt ggaaaaagca ttttttatta ctgattttcg ttataccgag caggcggcag
     7981 cgcaggctca agggtttgaa attattaaaa atgtcggacc tatttttgat gaggtagcaa
     8041 acttagtacg caaagaagaa ctgcactcat tagcttttga ggaaacaaca gtctcatttt
     8101 tagaatattc ggttttagaa gaaatcatcg atgcggaatt agtacctgtt tcacagatga
     8161 tcgaagaact cagagaagtg aaagatgaag aagaaatcgc tattatcgaa aaagcatgtc
     8221 atattgcgga tatggcatat gatcacatcc tgaaaatgat ccaaccgggc atgactgaga
     8281 ttgaagtcgc aaatcaacta gacttttata tgcgttctct tggcgcttca ggtgtttctt
     8341 ttgatacgat cgttgctagc ggattgcgtt cggctatgcc gcatggtgta gcaagcaaaa
     8401 aaatcatcga acaaggtgac ttgatcacat tggactttgg ttgctattac gaagggtacg
     8461 tttccgatat gacacgtaca ttcgctattg gtgatcctgg ggaaaaatta aaagaaattt
     8521 atcaaatagt cttagatgct caattagcag ttattgatgc tgcaaaacca ggaatgactg
     8581 gtgtccaatt agatgcagtt gctagagatt atatttcaaa acatggttac ggggaagcat
     8641 ttggacattc tacaggacat gggatcggtt tagagatcca cgaaggacca aacgtttctt
     8701 ctcgtgcaga aaagcaattt gtaccaggaa atgtgatcac tgatgaacca gggatctact
     8761 taccaggaat cggtggagta cgtattgagg atgacttgtt gatcacagaa gatggaaatc
     8821 gtgtgttgac tcgttcacca aaagaattga tcatcttata gtttcatcaa tagaagaagt
     8881 aagcgtaaaa atcagtgttc acagactttg ttatagaaaa tcactcgatt taatgataca
     8941 ctatagaaga acttttaagc tgtaagagat aataaagttg ggtgttttct ccaattcata
     9001 gcagtttgca gctgatcggc ggcagttttc ttaccacgtg atcactatgc cgcatcatag
     9061 agaattcgga tgtatctcgt gcttaggcaa agggaggaat aaataaatgg cagatgaaaa
     9121 aaatttgatt ttaaacgcaa accaagattt aggagagatc gtgatcgctc ctgaagtcat
     9181 cgaagtaatt atcgggattg cagcttctaa agtagaaggc gtatacggaa tgcgcggaac
     9241 ttttgccagc aatgtcactg aattattagg acgtgcagct cacggaaaag gtgtttactt
     9301 agcaaacgag gaagatggct tgaaagtaga tctttactgt tatctagaat atggtgtttc
     9361 agttcctaaa gtagcaatgg aaatgcagga acgggtcagc cagcaagtcc ttttcatgac
     9421 ggatatcgat ttatctgaag tcaatatcca tgtagtagct gttgttcctg aaaaattaga
     9481 acaaccaaca atcgaagaac tttttgaaga cgaagaggaa gagaatgaat aaagaattat
     9541 caagacacga gattcgggaa atggcattgc aggctttatt tccattagac ttcaatgcag
     9601 acctaacaaa agaagatgcg attttcaacg caatcgaatt agaccaccgc gacatgatca
     9661 acgaagatga atcagagttt gttcctgttt atcttgatac gctagttggc ggtgtctgtg
     9721 caaaaaaaga tgaattggat aaagtgattg aaaaacattt aaaaaacaat tggcacatca
     9781 atcggatctc aaaaatggat ttggtgatct tgcgtatcgc gatttttgaa atgacttatg
     9841 tgacagatgt accagcggct gttgctttaa atgaagcaat cgagttggca aaaacattta
     9901 gtgatgaccg ttcacgtaaa ttcgtcaacg gtgttttatc caatgtattg aaagaattag
     9961 aagctggggc gtaagtccta gcttttttct atgaatcatc ataaacctgt tataaatcgg
    10021 gaaaattcta tggtagaatg aaaacacaaa aaatatctgg gagtggttag atggcagaat
    10081 tgattaacgg aaaagaatta gctgaaaaaa tgcaggcaga gattgctgaa aaaatccaaa
    10141 aattgaaaga taatggtatc catcccggat tagtcgtttt attggtaggg gaaaatccag
    10201 ccagccaaat ctatgtaaga aataaagaac gtgtagcaaa agaaatcgga atccattcat
    10261 tggtagagcg ttatcctgag acgatttctg aagacgaact acttgcagaa attgaaaaat
    10321 acaatcaaga tcctagtttt catggtatat tggtccaatt accattgcca aagcatatag
    10381 atgaagaaaa gatcctgtta gctatccgcc ctgaaaaaga cgtagacggt tttcatccga
    10441 tgaatttagg cagactcctt gcgggtaaac cggacaagat tccttgtact ccatacggca
    10501 tcatgaaaat gtttgaagct tattctatcg atccggcggg taaacgagca gtagttatcg
    10561 gacgaagcaa tatcgtcggt aaaccgatgg ctcagctact cttgatggca gatgcaactg
    10621 ttactgttgc tcattctaaa accgaaaatc tggctgaact ggcacaagaa gcagatattt
    10681 tagtcgtagc aataggacgt ggacattttg tcacaaaaga attcgtaaaa ccaggtgctg
    10741 tagtgataga tgtagggatg aatcgtgatg ctaacggtaa gctgatcggt gatgtgaaat
    10801 ttgatgaagt ggagcctatt gcaggctgga tcactcctgt gccaaagggt gtagggccaa
    10861 tgactatcac gatgctgatg tatcaaaccg taaaatctgc cgaagctttt ggagcaggtg
    10921 aatagatggc acaagaatat ttaactgtca ctacattaac gaagtatttg aaaagaaaat
    10981 ttgatgccga tccttattta gaacgcgtat atttaacagg cgaaatatcg aatttccgtt
    11041 tacgtgccaa tgcccatcaa tatttcagct taaaagatga ccatgcaaaa atatctgcta
    11101 ttatgttcaa aggagcattc caaaaattac gattccagcc aaaagaagga atgaaagtcc
    11161 ttgtcgttgg gcgtatttca ttatatgaag cgggaggttc ttatcaaatc tatgtagagc
    11221 acatggagcc tgatggaata ggtgctttat atcaagaatt ggaagaacga aaagaaaagc
    11281 tgatgaagga aggccttttt caagggccta aaaaaatgct gccacgtttc ccaaagcgaa
    11341 tcgcggtcct taccagtcca agcggagcgg ttatccgcga tatcatcacg acggtgaaaa
    11401 gaagatatcc aattgtccaa ttggttttgt tcccaacagt tgtacaagga aaccaagcag
    11461 cggacaatgt tgtcaggaat atccagagag tagaagagat gggcaatttc gatacaatga
    11521 tcatcggtcg tgggggtggt tcgattgagg atttatggcc gtttaatgaa gagcgggttg
    11581 ctcgggcgat ttatcaagct aatacgccgg tgatttcatc tgtaggacat gaaacagata
    11641 ccacgatcgc tgatttagta gcagatgttc gggctgccac tccaacagca gctgcagaat
    11701 tagctgttcc tgttttgtca gaagagttga tgcgcattca agaacggcaa gctagattgc
    11761 agcaggcgtt tgtacgccag atccagcgca aacaagaacg gttcgagcgt gctcgacgtt
    11821 cctatgtatt tcggcagcct gaaagattgt atgaaggtca gaccatcaaa ttagaccagt
    11881 tgaaacagcg attatatcaa gctacacagc aaatttatca tgcgaaagaa aaacaaacga
    11941 tcgcattaag ccaccaattg gaacaagtgg ctccgcttta tcgagttaaa gcagctcgcc
    12001 aagaaactgc ttatctagaa aaacgcttag aagagaaaat ccaacaatat atgaaacaac
    12061 agcagcagcg ttttgctcaa gctgttcaat ctcttgattt gttgagtcca ttgaaaatca
    12121 tgggaagagg ctacagctat acgacgttaa atgatcaagt agtaaaaagt gtcaccgaga
    12181 ttcatcaagg ggatttgctg acgatccatt atcaagatgg aatcgttaaa ggacaagtac
    12241 aagagataga caaaggagaa taaaaatatg gaaaatccaa cttttgaaga gtcactgcaa
    12301 gaattagaaa aaattgtcat gcagttagaa caaggagacg ttccattaga atcagctcta
    12361 gattcgttta aacgaggaat ggaattaagc aaacattgcc aagatacatt agctaaagca
    12421 gaagagacct tgacgaaaat gatgacagaa tcaaatgaag aagttgattt tgacggaaac
    12481 gaggaaagct gagatgagat taactgactt ttcagctatt catctacctg ctgtagaaaa
    12541 agaaatcttg tcttttttag atgagcatac aacggataga ggactctatg atgcgatgag
    12601 ttattcagtg aaagcaggcg ggaaaagaat acgtccctta ttacttttaa cggccgttgc
    12661 ttcctttgat gagcctattg atgttcccgt ctatcaagta gcagctgctt tagaaatggt
    12721 gcatacgtat tccttgatcc acgatgattt gcctgcgatg gacaatgatg atcttcgtag
    12781 aggaaaacct accaatcata aagtattcgg cgaagcatta gctatattgg ccggagatgg
    12841 tttgcttaca ggagcattcc agctaatcag tatggcacac ttaggtaatt ctcctaagct
    12901 tctgctgctt cagcaattag ctgtttgtgc gggatcacaa ggtatggttg ctgggcaagc
    12961 tgcagatatc gagggcgaat cgaaaaagct ttctttagaa gaattagcat ttattcatga
    13021 aagaaagaca gggcatttga ttcgttatgc cttgctggct ggtggcatat tagcgaagca
    13081 acctgaagaa atcttattgc tacttcaacg attagccgaa catttaggtt tagcttttca
    13141 gataagagat gatctactcg atgtgattgg tacaacgaaa acattaggga aaacagctgg
    13201 taaagatgaa cgtatggaaa aaaatactta cccgcgttta ttaggcctag aaaagacacg
    13261 tgaggcgttg gagattgagc tgttatccgc caacaagatc atagacaaat tagaagagga
    13321 cgttttttct tttgatggcg ggctgatgcg ccagatgatc aagcaatttg atgtggagaa
    13381 atgagggaaa gaatggaaaa agaacgcgta gatgtattag cggtcaaaca aggattattt
    13441 gaaacaagag aacaagccaa acgttcagtg atggctggtt tgatttacaa cgaaaaaaac
    13501 gagcggtttg ataaaccggg agaaaaaatt ccagtgagta gtgaattaag agtcaaaggt
    13561 aaaaaacttc cttatgtttc tcgaggggga ttaaaattag aaaaagcact caaagcattt
    13621 gatctatctg ttgaaggaaa aacattgtta gatattggtg catccactgg cggatttact
    13681 gatgcagctt tgcaaaatgg cgcaaaaatg agttatgctt tagacgttgg ctacaatcag
    13741 ctagcttgga aaataagaca agatcctaga gtcgtcgtaa tggaaagagt gaattttcgt
    13801 tatgcaaaac ccgaagactt taccttagga attccagaag ttgctgttat cgatgtttct
    13861 tttatttcac taaaactgat gctgcctcca ttacatgcta ttttaaaaga agagggagaa
    13921 gtgattgctt taatcaaacc ccaattcgaa gcaggcagag aagctgtagg gaaaaacgga
    13981 attgttcgtg accctcagac ccatcaaaaa gttttagaag acattttgtc atttgcggca
    14041 caaggtggat atgatgtgct ggaattaagt tattctccga tcacgggggg agaaggaaat
    14101 atcgagttct tggctcactt gaggaaagta cccgaaagcg gaacaatcaa ttcagctatt
    14161 aatatggcag aagtggtttc aaatgcacac gaacagtttg accataaata aattgtaact
    14221 gtgaaaatca agtgagaagt atgcggattc ttctttatta taccaaggaa ataagctatc
    14281 attgattaag aagaatatgc taaggagcga agacgtaatg agaaaaaaag atcggcatcg
    14341 tttgattact cgattgttga gtgagcaaga tatccgaaag caggaagaat ttgttgaaat
    14401 cctgaaaagc aaaggaatat ctgttactca agctacgatt tccagagata taaaggaact
    14461 gaaactgatc aaggtaccgg cttcagatgg cggctaccgt tatagcatac cagctgaaac
    14521 aaacgaagat gtaaatacta aattggaaaa actattaaaa gacgctttcg tggcagttga
    14581 ccaaatggaa aaatttgtta ttttgaaaac attgccggga aatgcttcgg cagcagcaaa
    14641 tttgattgat aaacgttaca agaaagaatt attttcgatt atcaatgatg acgataatgt
    14701 tttgatgatt acacgaactg aagaccaggc agttttattg aagaaagact tcctgcatta
    14761 cctttaagtt gaggagcgaa tgtgatgcta caagaaatca gtattactaa ctttgcgatt
    14821 attccggaat tgcggctttc ttttcacgaa ggaatgaccg cgctgaccgg agagactggt
    14881 gcgggtaaat ccattattat cgatgcttta ggtctgttag caggaggaag aggttcaagc
    14941 gattatatac gtcaaggagc agaaaaatgt gtgcttgaag ggttgtttga actaccaaaa
    15001 caagaaggat tctctgaact gatggttgaa ttagggatcg aaacagatga ggacaatctg
    15061 attgttcgac gggatatgtc cttaactgga aaaaatgttt gtcgagtgaa tggtcatatt
    15121 atcacattag ctaatctgag aagaatcggc agttatttag tagatatcca aggacaaaac
    15181 gaacatcagg aactgctcca gcctgaatca catcttgctt tattggatcg ttttggagat
    15241 gtagctttcc aacagaagaa aaagagttat cagcaagaat atatctctta tcgtgagctg
    15301 gaaaaaaggg tacgaaaagt gcagcaaaat gaaaaatcct atgttcagag aatagatatg
    15361 ctccatttcc agcaagaaga aatagctgct gctgatttgc aagtcggaga agaagagaaa
    15421 ttaaaagaag aaagagaaaa acttagcaat tatcaaaaaa ttgctgatgg attagctgca
    15481 ggttacggag cactgacgaa taatgaacaa aacagtgtag atggtgttgg cttggccgtt
    15541 tcagaactgc aagggatcgc ccatctagat gtggagtatg aagcaattta tgaaaatatc
    15601 caaagtgctt actatttgct gcaagatgca atcggcgata tgagtcgtca gattgattta
    15661 ttggagttag atgagagtcg tttagaagaa gttacgcaac gtttagaatt aatccgacag
    15721 ttgaaacgta aatacggcga atccattgag tcaatattgg cttactatga cgaaatcacc
    15781 gaagagctag cttcttctga tttttcagaa ggtcaattgg acaaaatgaa agaggaacta
    15841 gagcaaaaag agcttctact tcagcaacaa gccgcagatc ttcatgaagc gcgtaaaaaa
    15901 atcgcaaaag aattggaaaa atccattctg catgaattga aaagtctgta tatggaaaac
    15961 actgagtttg aggtacgttt cttaaaagaa gaaaatagac aattgaatca tgatggattt
    16021 gaccaaatcg aattttacat tacgaccaat ccaggagagc cactgaagcc tttggtgaaa
    16081 gtcgcttctg gtggcgaact gtctcggatg ttgcttgctt tgaaaactat tttttcttct
    16141 gagcaaggtg tgacaagtat tatttttgat gaagtggata caggtgtgag cggaagagtt
    16201 gcacaggcca tcgcagataa aatttctcaa atatcgaaat actctcaagt tttgtgcatc
    16261 actcatctgc ctcaagttgc tgcagtcgcc gactatcaat attatattgt gaaagcagtg
    16321 attgatggac gtacgcaaac ttctgtttca gagttgaaaa caaaagaaag agaagaagaa
    16381 attgcacgta tgctggctgg aagtgagatt acaaaactca cactcgaaca tgcaaaagaa
    16441 ctattgaaac tcgcaaaaag gacctgagcc acctcgcagt ggttcaggtc cttttcagca
    16501 gctgatagct atattttatt tttacatgac actcattaca gcagtcaata caacagatcc
    16561 aatcgttaaa atcagcattg tccaaatgac aaattttgtt actttagcaa aagtacttgt
    16621 ttgttttttt tcacgcattg ttttcaacct cgtcattaat tagttgattc aagtgtacaa
    16681 tcttttttcc atttccacaa gagggctttt ttataaaaac ttcccttttt ttaaataata
    16741 ggtagttacg atacaaataa ataatgttaa tagaactagc catataaagg catcatccct
    16801 ttcagcaata ggtaacttaa tgttcattcc atagatactg ccgacgattg tcggtatagt
    16861 taaaataatc gttaaagaag ttaaaatttt cataatatta ttcagattat tcgatacgat
    16921 tgctgaaaaa gtctgattca tttgttctac tagttttaat tgaatgcgag aagtagtcat
    16981 tgcctgctct gtttccacca atacatcacg taaatgattt ttcaaagcat gattttcttg
    17041 gaaaagttta ttttgcgcta gttctttcaa tgcatctaaa ttagcagaag tcgcttctct
    17101 gaaataaacg aggctttttt ggatatccat gatttgataa agttgtttgt tttcagtgga
    17161 tacttgaagt ttggcttcca aagtatcgca ttgagattga agcgtagcta aataactgtt
    17221 gtaagagagt gtgacctgcc aaatgaattg aaagaagata ttgagattag gcgaaatatc
    17281 actttttgga aaggtctggc taaagacttt ctttaaaaac agtggttcat gattcgtaat
    17341 tgtgataagc ttctttttgg gcgtcaaaat aatagagagc gggtatgtat ccacttgaaa
    17401 ataaccgcta ggacttgttt tagcaaaagg atattgtaac aataataacg ctggctttgt
    17461 caactttgat tgattgaatt cttctgtacg ggaattttct gcatcgtcca atatggaagt
    17521 gatgtaatct gacggaattt caaattgatg tgtcagctga tgaatctctt cttgattagg
    17581 acgttctaca caataccaga tcgtttggtc agtttccttt ccggtattac gcagtttctc
    17641 attatctaat gtaaaatatt tgatcatgat tttcctcctg ttgcctgttt cttttattat
    17701 aacggatagg gaattggaat acatggaatg tgtttgtaag atatagaata taactgcata
    17761 aatttaaata aaatgaaaca gttgtgtaaa gtatatacag tatacttgaa gcaacgataa
    17821 aaaaggagtt tacgttcatg ggcacattca caccacttat cttgttttgg tatatttacc
    17881 cggtaatcgt tttgtttggt tgccaatttc ttgtaaaatt attttcgtta aatcgtcgtt
    17941 ttaaaataaa agcccctgat ttggcgattc cttttttgtg gggcggtatg catgcattgt
    18001 ctagaaatac agggacaatt tctatcttac cgtttttctt tatttctgtt ttgctaatgg
    18061 gaatttgtat tgctgttttc caagcatatt attatgaaga aattatttat ccgcgttatt
    18121 tcaagatgac ttggcgtttg gtttttctgt tgacaatcgt cttgtacgct gtcttgatca
    18181 ttgtgaacat actttcttac ctatgaaaaa aatagaaaag ctgtaaaaag tcttttaaaa
    18241 ggctttttgc agcttgattt ttaaaaaaat gtttatgaat aaaaaaagtt gttcccggat
    18301 ttcccagaaa aggtggtaga aagtggggga ttgtggtaga ctaattttag ttagtggagg
    18361 aatggggggc tttcaatgtt gatgggtgaa ttccaacata atattgatgc aaaaggacgt
    18421 ttaatcgtgc cctcaaaact tcgtgaagaa cttggcgaaa agtttgttct gacacgcgga
    18481 ttggatggct gtcttttcgg ctatccgatg agcgaatggg aaaatctgga agctaaactg
    18541 aacgaaatgc cgttggctaa aaaagacgca cgtacttttg tgcgattctt ctattcggct
    18601 gccactgaat gcgaacttga taaacaagga cgaatcaata tacccagcac gttaagaaat
    18661 tatgcagctt tgacaaaagg ttgcgtagtc atcggtgtat ctaaccgaat tgaaatttgg
    18721 gatgagacac gatggcaaga tttttctgca gcagcagaag agaactttga cgaaattgct
    18781 gaaagtatga ttgatttcgg actataagaa taggaagagg ctgactaatg actgaaacat
    18841 ttcaacacta tacagtcatg ttgaaagaaa cggtagatgg attgaacatc aaaccggatg
    18901 gcgtttatgt agactgtaca ctcggcggtg ccggacatag tgaatattta ttaagccaac
    18961 taagttctaa agggcatctg tatgcgtttg accaagatca aaaagcaatc gatcacgcca
    19021 agctgcgttt agcttcttat gtcgaacaag gtatggtcac atttatcaaa gcgaacttca
    19081 gagaattaga agaacgcttg agtgagcatg tagataaggt agacggcatt ttgtatgatc
    19141 taggggtttc ctctcctcag ctagatgaag cagaacgtgg atttagttat catcaagatg
    19201 caccgttgga tatgcgcatg gatcagtcag ctcctttatc cgcttatcac gtaattaatg
    19261 aatattccta ccatgaatta gtaaagattt tcttccgcta tggcgaggag aaattttcca
    19321 aacaaattgc tcgtgaaatc gaacgagtac gaaaaacaaa accaatccaa acaactggtg
    19381 agcttgtcga aatcattaaa caagtcatcc cagctcccgc aagaagaaaa ggcgggcatc
    19441 ctgcaaaaag aatttttcaa gctgtgcgga ttgcagtaaa tgacgaactc ggtgccgaag
    19501 aggcctcttt agaacaggca atccggttat tgaaagtcaa tggaagaatc agcgtaatca
    19561 cattccattc attggaagac agaattgtca aaagtatgtt taaagaatat agtacggtac
    19621 aagatctacc accgggactt cctgtcatac ctgacgaatt ccaaccagaa ctgaaagtca
    19681 ttactcgtaa accaatcctt ccttcagagg aagagctaac agaaaacaat cgatcacgca
    19741 gtgcaaagtt acgtattgca gaaaaaataa gaatgaaaga ggagtagaat gatggcagaa
    19801 ttaaagaaaa tgcaagatta cgaatatgat ttacctgaat tggtggatca gccggaacaa
    19861 caaatgcctg attcttcttc gaatcaagaa atcattcttc cacaatcacc ggcaaaacga
    19921 ttaaagcaga tttctgcttt ggaaaaaatt gctgtggcaa gcgtggcgat tgctattgtt
    19981 gctttatgtg ttttaacagt tatgctgcgc acaaatatca gcggagctga aaaagaaatc
    20041 acgtcaatac aaacagaaat caatgataaa aagcaagaaa aaacgagttt agaacaagaa
    20101 aaaaacgaac tttctcgtac tgaacggatt aaacaaatcg cagaagaaaa aggattgtct
    20161 attaatgacg acaatttaag gaaagtgaag taaatgagtc ttaaaaataa aattcggcgg
    20221 tttatggaaa agaaaaatct aaatccgatg aacaatcgca aaaaagtagg tatcatttta
    20281 tttgctacga gtattggatt gttcttttta tttgccgtcc gtttctcgta tatcgttatt
    20341 ggaggtcatg ttgctggtac ctcattagct gaaaaaacaa aacagttgta ccaaggaagt
    20401 gaagtagtaa aagcaaaaag aggaacgatt tatgatcgta acggtgtggc gctcgcagaa
    20461 gatgcctcct cttattcaat taaagcgata ctctcaaaaa catatacctc tggggataaa
    20521 aaactgtatg ttgaagaaaa gaattttgat aaaatagcag aaatcctgca taagaatctt
    20581 tctatagaca aaaaagatgc actgaatatt ttagaggacg gcgcaaaaaa agagctgtat
    20641 caagttgaat ttggtagcta tgggaaaaat atctcccaag aaaccaagca aaacatcgaa
    20701 gcggacatga aaaaagaagg tgtcgctggt ctttattttg tcgaccatca ggctcggatg
    20761 tatccaaatg gcgtcttttc ttctcatttt attggatacg cagttccaga caaagatgag
    20821 aatggcttag ttggtaaact aggtttggag tctgcttaca atgatatctt gagcggtaaa
    20881 gatggtaaga ttatttatca aaaagataat tttcaaaatc ctctgccagg tacagtggca
    20941 gaagaagaaa aagctgtcga cggacaagat atctacacga ctttagacag cagactacaa
    21001 agttatttag aaacactgat ggatcaagta aatgaagaat atcagccaga agaattgaca
    21061 gcagtcctga tgaaagcaaa aaccggagaa atcttggcaa tgggacaacg acctactttc
    21121 aatcctgaaa caatggaagg attgactgga aaagatgcta tctggcgtaa tttcttggta
    21181 caagatagtt atgaaccagg atcaaccatg aaagttttca ctactgcagc tgctatcgaa
    21241 gaaggagaat tcaacgagaa tgaaaccttc caatctggaa aaatccaagt agaagatgct
    21301 acgatcaatg accatgactt tggtgaaaaa ggtgtgctga ccatgcgtca agctctttca
    21361 tggtcaagta acgtcgggat ggtcatactg gaacagcgat taggcggacg atggtataac
    21421 tatttacaaa aactgggatt tggacaaagt actcattctg gattagatga tgaagtaaac
    21481 ggtgctttac ctacattaaa tattgtcgac cgagcaatga gtgcttatgg acaagcggta
    21541 ggtgtgacga atttccaaat gatgaaagct ttcacttcta ttgcgaataa cggtacaatg
    21601 attcaacctc gatatatcag caaagtggtg gatccccaaa ctggagaaga gcgaacaact
    21661 caaacagaag ttctagggca acctttttca aaagaaacaa cagaaaaagt ccgtgaatat
    21721 atgagagatg ttgtcgaaag cgaaaactac ggtagtgctt atggtgtata tagtgtacct
    21781 ggatacaatg tatcggcaaa aaacggaaca gcccaaatcg cttctgatac agggggatat
    21841 caaacaggag atacagccta cctgtattcg attgtggaga tggttccttc tgaagatcca
    21901 gactatgtct tgtatctaac aatgaaacac cctaagacat atgatcgaat ggcattggct
    21961 aaaatcgcaa atccattgat gaaacgggca atggatttta aagaaactga agaagattca
    22021 gatacagaaa caaaaacaga aaaagtatcc gtagccgatt atcgtaatct tgaagcggac
    22081 gttgcagcag ctgatgctca aaaaagtgga ctgcaacctg tagtgatcgg taatggtaaa
    22141 aaagtacaaa aacaatctac tgccaacggt gatcagttga tctctggaga aaaactgatc
    22201 ctatatactg gtggtgataa gctgatgcct gacgtaacag gatggtcaaa agcagatatc
    22261 atgaagctgg ggaaaatcct cggtattgaa gttagttttg atggagatgg ttattgtgtc
    22321 aaacaagaac ttgctccata tgaaaaaatc actgaagaca aacttagttt tactttagaa
    22381 gaataagaag agaaatagga gagaaaagag catggaatgg acacaagcat tgattcccat
    22441 tgtcagcagt tgtgcaatga caattgccgc aatgccgcta tttattggtt attttcaaat
    22501 gaaaaagcaa ggtcaggcga ttcgtgagga gggaccaaag tggcacaata gcaaagccgg
    22561 aactcctaca atgggcggct tagtgtttct gatcggctct attttgacag gaatttgggt
    22621 tggagcttgg caaaaacagc taactcctac tcttttcatc ttgctttttg tattagcgtt
    22681 atacggagta atcggctttc ttgatgattt tataaaaata ttcaagaaaa gaaacatggg
    22741 actgaactcg aagcagaaat tgattggaca gattctaggg ggaatcatct tttatcttgt
    22801 ttaccgttct gaaggttatc caggtgtatt gaatttcttc ggactagatc tacctttagg
    22861 aatcatttat ggtattttcg ctctcttctg gcttgttgga ttttcaaatg cagtaaatct
    22921 gacagatggc atcgatggat tagttgctgg cttaggaagt atctcttttg cagcttacgg
    22981 tctaattgct tggcatcagc atcagtatga tgtattaatc atttgcttaa gtgttttagg
    23041 aggtttactg ggtttcttcg tctacaacag aaaacctgcc aagattttta tgggagatgt
    23101 cggttcatta gcgttgggcg gacttttagc ggcaatttct ataatgctaa accaagaatg
    23161 gacattgctt ttgattggtt tgatctatgt tatggaaaca gcaagtgtga tgcttcaagt
    23221 tacttccttt aagcttacag gaaaacgaat ttttaaaatg tctccaattc atcatcactt
    23281 tgaaatgtgc ggatggtcgg agtggaagat tgatacagtt ttttggttga tcagcattgt
    23341 gacttcgttg atcacattat ggatcgtttg gtaggagatg acgggaaatg aaaaaaatat
    23401 caacatatga aaataagaaa gtgcttgtct taggactcgc aaaaagcgga gtaagtgcgg
    23461 ccaaattatt acatgaattg ggcgcacttg tcaccgttaa tgatggaaaa ccttttgacg
    23521 aaaatccgga agcacaagag ctgctttctt tagggataaa agtaataaca ggtagtcatc
    23581 caatcgaatt gttggatgaa gaattttcat tgatggtcaa aaatccaggg attccctaca
    23641 gtcatccttt agtagcaaaa gcccaagaaa tgggtattcc ggtaattacg gaagttgagc
    23701 tagcttacga agtagcggaa tgcccaatta ttgggattac aggaacgaac ggaaaaacaa
    23761 cgacaacaac gatgacagga ctactactga atgcaggagc agatcaagga attgcacgtt
    23821 tagcgggtaa tattgggtat ccagcaagtg gtgtagcgca agaagcaaaa tcagaagata
    23881 aaattgtgat ggaattgtct agcttccaac tcatgggaat cacagatttc cgccctcata
    23941 tagcggtgat taccaatatc tatgaagcac atattgatta tcatggtaca agaaaagaat
    24001 atgtcaaagc taaatggaac cttcaaaaaa acatgacaga aaaagattat ctgatcctta
    24061 actggaatca gtcagaacta caagaattag cgcaaagaac aaaagcacgc gtactgcctt
    24121 tctcgacaaa agaagtgttg gaagacggtg tgtatgctga cgattactca atatactata
    24181 aaaaagaaaa aataatggaa atatctgaac ttggcgtacc aggtaagcat aatgtagaaa
    24241 atgcactagc tgctatttct gtagcaaaat tgtatggtat atcaaatgaa gctatacgag
    24301 aaacgttgca ttttttccat ggcgtaccac atcgtacgca atatgtagga gaaatccaag
    24361 gcagaaaatt ttataatgat tcaaaagcaa ccaatatttt agcgactaaa atggctctaa
    24421 gtggatttga aacgtctaag gtcgtgttat tggctggcgg attagacaga ggaaatacat
    24481 ttgatgaatt aattccttca ttaaaaggaa tcaaagcaat ggtcgtattt ggccaaacaa
    24541 aagaaaagtt gatggatgct ggaaaaaaag caggcatcga gacaatagta acagccgatt
    24601 ctgtagaaca agcagttcct ttagcattag aaaacagtac agatggtgat gtagttttat
    24661 tatcaccagc gaatgccagc tgggatcaat acccaaattt tgaaactaga ggaaacagat
    24721 tcatggaagc tgtgaaccga ttaaaataga aaaagtagaa gcgagcgata aaaaatgaaa
    24781 attttggtta caggcggagg aacaggggga catatttatc cagctcttgc ctttgtcaat
    24841 tatgtaaaga ctcaagaacc gaatgctgaa ttcatgtatg tgggagcaaa acgaggtcta
    24901 gaaaacaaaa tcgtccctga tacaggaatg cctttccata ccttggagat tcaaggattt
    24961 aaacgaaaaa tttccatgca taacatcaag acggtccgat tgtttcttaa gagtatccgt
    25021 caagcaaaaa aaatcctcag tgattttcaa ccagatatcg ttatcggtac tggaggatat
    25081 gtttccggag cggtagtgta cgctgcagcc aaaatgggta ttccaacagt tatccatgaa
    25141 caaaacagtg taccaggtgt taccaataaa tttttaagcc gatatgtgga tcggatcgct
    25201 ctttcattta aagatgcggc ttcctttttt cctgaaaaca aagcagttct tgtaggtaat
    25261 cctcgagcac aagaagtagc agatactaaa aaatcagata ttttgaaaac atttgatttg
    25321 gatccagaaa agaaaacagt cttgattttt ggcggtagtc aaggagcact taaaatcaat
    25381 caagcagtat cagttttttt aagtgaatat attccttctt cttaccaagt tttgtatgct
    25441 tcaggagata gatattatga agagatccta aataaaataa atcctgaaat acaaaatatt
    25501 agtgtccagc cttatatcaa aaatatggca gaggtcatgg cttccagtga tctgctggtg
    25561 gggcgtgctg gtgcaacttc gatcgcagaa ttcacagctc tggggttacc ggcagtgttg
    25621 gttcccagcc catatgttac gaatgatcat cagactaaaa atgcaatgag tcttgttcac
    25681 gcaggagcag caaaaatgat tgcagataat gaactaacag gtgaatcact aagtcagaca
    25741 gtcaacgaaa tcatgggtga tgaagaattg caaaagcaaa tgtgccgagc ttctaaagaa
    25801 caaggaatcc ctgatgcttc aaaacgtctt tatgacttag taaaacaaat aatcaaaaaa
    25861 taaaggcggt ggtcttagtg agtaagaaaa aagaaccaaa aaaattgtct catccgtctt
    25921 ctgatgaaaa caatttgact ccttggcaaa aggctaaccg aaaatatctg gcagaacaag
    25981 agatgaaaaa ccaagagcca aaaaaagaag acataacatt agatacaccc ctttcagatt
    26041 ctgaagaaga aaaaacggtt caagaggaac agcaagaaga aagccagtcc gagaaaaaaa
    26101 tcgaattttt tgaagagatc aagcaaaaga aaaacggtgg accatataac ggttcttttt
    26161 tgaaccggct gcccaattta aaaagtcaac ggcacaaagt actatttcgt cggttagctt
    26221 tgatcatcac tgtattgggt ataccgctga tttttttgat ctattatgtt tctccttata
    26281 gcaaattaca agcagttgca gtatcaggca ataagacagt caacagccaa gaaatcatct
    26341 ctgatacaaa attgtcatta ggagaaaatg tttggggaca gtattttcat cgatctactt
    26401 atatcgatcg ccttaaaaaa gcacagccaa gaatcgaaac agcaaatata catttcaaag
    26461 ggatgaatga attcgatttg gatgtaacag aatataaaga gattgctttg atcgcaaaaa
    26521 acaatcaata ctacccagtg attgaaaatg gaacagtatt agatgaaaaa gtagcaaatc
    26581 ctacaaaaaa tctccctatc ttggaagagt tcaaagactc tgcaaaaatc aaagaattaa
    26641 caaagcaata caatcagtta tcatcagaat tacaaaaagc aatttctgaa atcaagtaca
    26701 cgccaagagc aagcaataaa aatctgatcc aattgaatat gaatgacggc aatcaagtta
    26761 ttgtaaatat caataatctt gcgaaccaaa tgaaatatta ttcgcaagta gccaaagata
    26821 tggatgaaaa aggtgtgatc gacatggaag ttggtatctt ctcgcatcct tacggtagca
    26881 ctcaagaaac ggaagaatcg ggccaatcgg aagaaacaac tgaaagtgaa gaaaatgcgg
    26941 atcaaacaaa cgaatcaagt gaagaggcag aaacgcaaaa agcacaaaca cctggatcgt
    27001 ctagcacaaa cggatcatct gattcaaatg attcaagtga aacaagtcaa atagaagata
    27061 atccaagtac aactccgtca agtttaaatg aaaattgata aaacaacttt aaaaagtaag
    27121 aatgtacttt ctaaaaacca gtaatttatg gtagaattgg aagcagtgaa tatatctatt
    27181 aataagtata ttttaaaacg gacgctagtt gttcgcttgt atatggaata gtaagtagga
    27241 gggaatccca ttcatggcaa aaacaggaat gtatgtaggc cttgatattg gaacgacatc
    27301 tgttaaagtt gtcgtggctg aatatatcga cagccaaatg aatattattg gagtaggaaa
    27361 cgcaaaatca gaagggatta accgaggcat tatcgttgat atcgataaaa cggtccaagc
    27421 gatacaacga gcagtacgac aagcagaaga aaaagcaggt atccagatca aaggcgtaag
    27481 tgtcggtttg ccagcaaaca tgcttgaagt agaaaattgc cagggaatga ttgcagttaa
    27541 cggagattca aaggaaatca ccgatgaaga tgtcagaaat gtggcttcag ctgcccttgt
    27601 tcgttcaata cctcctgaac gtcaaatcgt gtcaatattg ccacaagatt ttactgtcga
    27661 tgggttcgag ggaatcaaag atcctcgtgg aatgatcggc gtaagattag aaatgtacgg
    27721 tctgttgttt acaggaccta aaacgatcat ccataacata cgtaaatgcg tggaaaatgc
    27781 cggattaatc gtcaacgaaa tggtgattac tcctttagcg ctgactgaat caattctttc
    27841 tgacggagaa aaagattttg gtacgatcgt cattgatatg ggtggcggcc aaacaacaac
    27901 ggctgttatg catgataagc aattgaagtt taccaatctt gaccaagaag gtggcgaatt
    27961 tgtcactaaa gatatctcta ttgtattaaa tacgtcattt aacaatgcag aagcgttgaa
    28021 aataaactac ggggatgcat acccagaacg gacatcacca gatgaagaat ttccagtaga
    28081 tgtgattggt cagtcagaac cagtgaaagt cgatgaacgc tacttgtcag aaattatttc
    28141 agctcgaatg gaacagattt tcaacaaagc aaaagatgca ttggaccaaa tcgaagcatt
    28201 agagttgcca ggtggcgtcg tattgacagg cggagctgct agtcttcctg gtgttgtgga
    28261 cttggcacaa gaaatcttcg gcgtaaatgt aaaattgtat gttccaaatc acatggggct
    28321 gagaaacccg gtatttacaa atgtaataag tatcgtggat tattcagcaa acttgagtga
    28381 agtgtatcaa ttggcaaaaa ttgctgttac gggtgaaacc tcagcagctc gtcaaatggt
    28441 agttgaacaa gaaacaacaa acacatatga gagctatgaa gcacctgaag aaacaatcta
    28501 cgatgagccg gaaccaaaag agtccggaga aaatgtaaaa aataaaatca agggtttctt
    28561 tacaaatatt tttgattaat tcatcctggg aggaataaga tatggaattc tcaattgata
    28621 ataatatcaa tgacggcgca gtaatcaaag tcatcggagt cggtggtggc ggtggaaatg
    28681 ctgtaaaccg aatgattgaa gaaaacgtta aaggtgttga attcattaca gccaacacag
    28741 acgtgcaagc attaaaaaat tcaaaagctg aaacagttat ccaattaggc cccaaatata
    28801 ctcgtggttt aggtgctggt tctcagccag aagttggaca aaaagcggca gaagaaagtg
    28861 aacaatcctt acgcgaagct ttagacggtg cagatatgat tttcatcact gcaggaatgg
    28921 gtggaggaac cggtacaggt gctgcaccaa tcgttgcagg tatcgcgaaa gaattgggcg
    28981 cattgactgt tggtgtcgtg acacgtccat tcacttttga aggaccgaaa cgtggccgtt
    29041 ttgctgcaga aggtatcgct cgtttgaaag aaaatgtgga tacattatta attatctcaa
    29101 ataaccgttt attagaagtt gtagataaaa agacaccgat gcttgaagct ttccgtgaag
    29161 cagataatgt tttacgacaa ggtgtacaag gtatctctga tttgatcaca gcgccaggtt
    29221 acgttaacct tgactttgct gacgtgaaaa cagtgatgga aaaccaaggt acagcattga
    29281 tggggatcgg tgtggctagt ggagaagatc gcgtagtaga agcaacgaaa aaagcaatct
    29341 cctctccatt gcttgaaaca tctattgatg gagctgaaca agtacttctt aacatcacag
    29401 gcggtttgga tatgacattg ttcgaagcac aagatgcttc agatatcgta gcaaatgcag
    29461 caactggcga tgtaaatatc attctaggta catctatcaa tgaagaaatg ggcgatgaaa
    29521 tccgtgtgac ggtcatcgca acaggtatcg atgaatcaaa aaaggaacgc aagtctagcc
    29581 gaccagctag acaagcacaa atgcaatcac ctgctcaaaa gactgtatta gatatggatc
    29641 aagctaaacc aacttcttca gaagaagaaa acagttttgg cgattgggat atcagacgtg
    29701 aacaaaatgt tcgtcctaga gttgatgatt ccaattttga caatatcgaa aaaaaagaat
    29761 ttgatacatt caaccgcgaa gaaacaaaat ctcatggcga tgatgaatta aatacgccac
    29821 cgtttttccg tcgtaaaaaa taaaggagag gaagagcaca tgattgctga caacttgcag
    29881 aaaatcaagc aagaaattca ggattcgtgt gctcttgttc atcgtgatcc gaaagaagtt
    29941 acattagtag ctgtaacaaa atctgttgaa tccgctcaag ctgaagaatt ggttagagct
    30001 ggcgttatga attgtgcaga aaaccgagta gataaattat tagaaaagaa acaagattta
    30061 tttgattttc ctgaaataaa gtggcattta attggtaatt tacagcgcag aaaggtaaaa
    30121 ttgattgtaa atgaaattga ttactttcat gcgttggaca gtttaagatt agcagaagaa
    30181 atccagaagc ggttagagaa agaattatcc tgttttattg aagtaaatgt ttctggtgag
    30241 gaatcaaaac atggaatttc accagatgag cttttaccgt ttgtggaatc tttagctgcc
    30301 tgcacaaaaa tcaaggttgt tgggttgatg actatggcgc ctaaagaagc ttctgatgag
    30361 caaatccata aagtgtttgg tacgctgaaa gaattgcgtg atcttgttca agcaaaaggt
    30421 tatgcccatg caccatgtag tgaattaagt atggggatga gtcaagactt tcaaattgcg
    30481 attgaagaag gtgcaacatt tatccgtgta ggaacagcgc tattcaaaga cgaataaagg
    30541 aggaacaaaa atgccgcttt taaacagagg agcaattgct aattttttcg gattagctga
    30601 tgaggaagag tacgaatacc aagattatcc tgaaacaaca aaaaaggctg tacagccaac
    30661 taaaaaaaat acaacacaga atcaatcaac tcattctgta tctgctgcga atactcatga
    30721 aaaacaaaaa acggcgactt cacgtcctgt aacgcatgaa agtattcagc cggctgaaaa
    30781 gaaaaaagta attgaactac atcaatcacc tacaaaaact gtagcgaaag aaagaacagc
    30841 ttcaagaacc tctacacaac ctaaagcaaa tcttgctgga aaaatcactg taatggaacc
    30901 aagagcttat tctgaagcta tggcgattgc taaacgaatc attgctggag aagcaacctt
    30961 agtgaatttt catttagtag aagaaaaaca agcgagaaga atcgttgatt ttctaacagg
    31021 aacagtttat gcactagatg gagatatcaa acgtgttgga gatgagattt ttctttgtac
    31081 accgactggg atggagattg acagttcaac cgctcaatct tttgcagatg cgaatttgtt
    31141 tgatctgtaa gaggaggaac tacgatttat actattgcac tattacttca tttacttcaa
    31201 cgggcagcct atttctatag cattatcttg atggtatatg cattgctctc ttggtttcca
    31261 ggcgggtatc aatcagggct aggacgatta ttagctaaga tctgcgaacc gtatatcagt
    31321 ttgttcgatc gtctgccttt gaggattgga cccttggatt tcacaatttt aggggcaatc
    31381 ctgatcttga acttagccgt acaaggttta gcacagatcg ttatatggat aactgtccat
    31441 ttagtttatt gattgaagca acaaaatctt tattctcaga atagatagga ggggaaaatt
    31501 ttggatgcaa atgtatacca gcactttcgg gcagatgaac gtacatttat cgattcggta
    31561 ggcgattgga ttgaacaggt tcaaggacaa tatgcaccct atctgaccga atttcttgac
    31621 ccaagacaat cctatatttt agaaacactg attcgacaaa gcagtgattt gcgtttcatg
    31681 ttttatggcg gatatgaaca ggcagaaaga aaaagatgtt tgattttccc cgattattac
    31741 gaacctgtag aggaagattt tgaaacagca ttgtatgaaa tccattatcc tagcaaattt
    31801 gccagcctaa gtcatgggaa aatccttgga actttagtgg gaacggggat caaacgctct
    31861 tactttggcg atatcatctc ggacggtgat cgctggcaag tatttattgc aaatgaggtg
    31921 gatcattttg ttgctgcaca agtaacaaag attggaaagg ttgctgtacg cttggaacca
    31981 atcaaataca cagaaatgat tatgccgaaa gatagctgga cacaagaaag aggaacagtt
    32041 agttccatgc gactagacag tgtgatctct gagatttata atatttccag acagcggtcc
    32101 aagcagctga tcgaagctgg taaagtaaaa gtgaactgga cagaaaatac aagacctgat
    32161 tttgtacttg aactattaga tattatttca gtcagaggtt atggcagagt ccagatccaa
    32221 gaacttgagg ggaagacgaa aaaagaaaag tacagggtac tgttcggtgt attacgtaaa
    32281 taaaataaaa aggtggttta ctcatggctt taactccatt agatatccaa aataagacat
    32341 tcccgacaaa aatgcgggga tataatcaag acgaagtaga tgatttctta gatttagttg
    32401 ttcgtgacta tgaggaattg actcaaagaa atcgagaatt agaaaaagca gtcaaacatt
    32461 ctgaagaaaa acttgagtac ttcaatgaat taaaagatgc tttgaaccaa tcaatcatcg
    32521 tagctcaaga tacggctgat aaagtaaaaa caagtgcaag caaggaatcc gaagtgatcg
    32581 ttacttctgc acaaaacaaa gcagatgagt tagttgcgaa cgctgaaaaa cgtgcacatc
    32641 aattaacaac tgatgcagaa gaaaaagcaa gaaaaatctt gacagatgca acagaaaaag
    32701 caagacagct tgcgacagaa actgaagatc ttaagaaaaa aacacgagta ttccatcaac
    32761 gtatcagctt gatgcttgag tctcaattag aacaagtgaa aagtccggaa tgggatgaaa
    32821 ttttacaacc attctccagc tacgtgactg acagccatga agtgatcaaa gaagttttag
    32881 caaaacagct tgacaatgaa aatgatactg atgtaaactc agacacagga gtcatagaag
    32941 cacctatgac ggcatttgat acacaggcta ttgatatctc gatcgtgcct ggtgaaaatg
    33001 aaaacgagta gaacagaaaa gcactttgtt attcttaaag cgagccgatg agagtggaag
    33061 ttcggtaaga cactgcatgg tgaaagatcc tctacttgag acctgctttt ccgaaattag
    33121 agtaagaagc agcggctgac ctcgttattg gtcacagaga gtttgcacta gatagatgca
    33181 actaaatttg ggtggtacca cggcatgttc gtcccttgcg gctgaacatg cttttttgtg
    33241 tccggaaaac aaataaagga gtgtatggtt gaatgaaaat gaaagaaacc ttgcaattag
    33301 gaaaaacaaa attccctatg cgtggtaatt tgccaaaccg agaaggcgag tggcaaaaag
    33361 aatgggaaga aaatcgaatc taccaaaaaa gacaagagtt gaacgaagga aaacctagct
    33421 ttgttcttca tgatggacct ccatatgcca atggtaatat ccatttagga cattcattga
    33481 ataaaatcag taaagacatt atcattcgtt caaaatcaat gtctggctat cgtgcaccat
    33541 atgttccagg atgggataca catggattgc cgattgaaca agtattggct aataaaggcg
    33601 tgaaacgtaa agaaatgtcc atggcagaat acttggaaaa atgtcgtgaa tatgcacttt
    33661 cccaagtaga taagcaacga gcagatttca aacgcctagg agttgcagga gattgggaac
    33721 atccttatgt tacattagat ccttcttatg aagctgcaca agtacgcgta tttggtaaaa
    33781 tggctgaaaa aggctatatt tacaaaggat tgaaaccaat ttactggtct ccgtcaagtg
    33841 agtcttcttt agcggaagct gaaattgaat ataaagatgt aaaatctcca tctatttttg
    33901 tagctttcaa tgtcaaagac ggcaaagata ttcttgatga agatacagct tttgtcattt
    33961 ggacgacaac accatggaca ttgccagcaa accaaggtat tgctgtaaat ccaaaattta
    34021 cgtatgtatt ggtggaagcc gatggccgta agtttgtcgt agcgaaagat ttgctagaaa
    34081 cagtccaatc agaaatcggt tgggaaaatg taacagttct aaaagaattt gctggacaag
    34141 aaatggaata tatgactgca agtcatccat tctatgaacg tgaatcactt gtgattttag
    34201 gcgatcatgt tactttagac gctggtactg gattagtcca tactgctcca ggacatgggg
    34261 aagatgacta tatcgctggt agcaaatata aactatctgt agtgtcacct gtagactcaa
    34321 aaggattgtt tacagaagaa gcacctggat ttgaaggtat cttttatgac aaagcaaatc
    34381 ctatgatcac tgatctatta aaagaaaaag gagctttgct gaagctagat ttctttactc
    34441 atagttatcc tcacgattgg cggacgaaaa agcctgtgat ctatcgggca actccacaat
    34501 ggtttgcatc tatcgataaa ttccgtcaga atattttgga tgaagtagaa aaagtagagt
    34561 gggttatccc atggggtaaa acacgtttgt ataatatgat ccgtgaccgc ggcgattggg
    34621 taatttcccg tcagcgtgca tggggagtac cacttccgat tttctatgct gaaaatggag
    34681 aagcaatcat cacaccagaa acaatcgaac atgtggcaaa attatttgct gaacatggat
    34741 caattatctg gtttgaaaaa gaagcgaaag aacttcttcc tgaaggattt acccacccag
    34801 gttctcctaa tggcgaattt acaaaagaaa aagatatcat ggatgtctgg tttgattctg
    34861 gttcttctca tgaagctgtt ttacgtcagc gaccagaatt atcattccca gcagatatgt
    34921 atttagaagg atcagaccaa tatcgtggct ggttcaactc aagtatcacg actagtgtgg
    34981 ctatcaatgg tgtagcacca tacaaatcag ttatttctca aggatttgct ttagatggtg
    35041 aaggccgtaa gatgagtaaa tctttaggaa acatcattgc accagaaaaa gtgatcaaac
    35101 aatttggtgc agatatcctt cgcttatggg tatctagtgt agattatgaa gcagatgttc
    35161 gtgtttctat ggatatctta agtcaagtgg cagaagtata tcgtaaaatc agaaatacga
    35221 tgcgtttctt attagcgaat acagaagatt tcgatcctaa aaaagatact gtttcgtaca
    35281 atgatttacg ttctgtagac aaatatatga ccgttcgatt gaaccaagta atcaaagaaa
    35341 tccgagaaga agggtacgac aaatataact tcatgcatat ttaccgtaca gttatgaact
    35401 tcttgacggt tgatctatcg tcattctacc tggattttgc taaagatgta gtttatattg
    35461 aagcagaaga tgattatcaa cgtcgttgca tgcaaactgt tttctatcaa acagctgtag
    35521 ctttgacaaa attattgact cctatcattc cgcatacagc agaagaaatc tggtcattcc
    35581 tgaaagaaga agaggagtac gttcagttaa gcgagtttcc tggttatgaa gagtttgcaa
    35641 accaagaaga attgatggat acctggactg ctttcatgga cttccgtgat aatgtcttaa
    35701 aagcattgga agaagcaaga aacagcaaat tgattggtaa atctttggaa gcaaaactaa
    35761 ctgtttatcc aaaacaacaa gttcgtgaaa tgttaaaagc tttggatgca gatattgcac
    35821 aattattgat cgtttcacca gactactttg aagttatggc agcagatgaa caagtgccag
    35881 ataacgctat ggtatttgat gatgttgcta ttacagtgga aaaagcagac ggggaaactt
    35941 gcgatcgctg tcgccaagtt cgtaaagatg taggagtaga tgaaaaactt cctcatctat
    36001 gcggtcgttg tgcaaaaatc gttgaagact tctatcctga agctgtagct gaaggattcg
    36061 aagaaaagta aaagggaaaa gggggtgtga catgttcttt gtcacacccc cttttgttat
    36121 caatcgttta attttccacg ttcacgatac cagttatctg gttgccagat ccattcgaat
    36181 ccgtcttttt caagtagttc gaaagctgct ttaggtccca ttgatccagc tttgtagttg
    36241 ggaaaatccg gttgtgtttg gtcccaagct tttcgaatga catccacgat atgccaagat
    36301 tgagcgactt cttcccaatg agagaagttc gttccatcac cattcaacgc atctaatagt
    36361 aatttttcgt aagcttctgg gctattttca accatttctg cactatttct aaattccaat
    36421 ttgattggtt ccgtttcaaa tccttgccct acttctttcc cattcaagga taaagaaaat
    36481 ccttcagttg gctgaatata gatcgttaga acattaggtg gaagtgtcgt atcgtcacat
    36541 ggttcatcga cagatgtttt gaatacatta acaggaactt gtttgaatac gatgttgatc
    36601 cgagtccctt tttcagtcaa acgtttgcct gtacgtacat agaaaggaac acctgaccaa
    36661 cggaagttat cgatagtgaa tttgcctgcg acgaatgttt cagtctctga ttgagcatcc
    36721 actttgtctt cttcgcgata gcccttgaat tgtacaccat ccaattcacc ttgagcatat
    36781 tgcccgcgaa cgaagttttc taacacttct tcttcgttat aaatgcggat agctttcaat
    36841 gctttgatct tttctgtacg gatttctttt tcggaaaatg ctactggcgg ttccattgca
    36901 agtaatgcta caacctgtaa aatatgattt tgcaccatgt ctttaagtgc gccactatga
    36961 tcgtagtagc ccccgcgatc ttctacaccg aggttttcgc caaaagtaat ttggatattg
    37021 tcgatatagc gattgttcca ttgagcctca aaaatattat tggcaaaacg gatagctgag
    37081 atgttttgga tcatttcttt tcctaaataa tgatcaatcc tgaaaatatc ttgttctggg
    37141 aatacttctc tgatttgctg gttcaattca aaagcagact ggtaatcaga accgaacggt
    37201 ttttctatga tcaaacgatt gaagccattt tttgtcataa tattttgtga tttcaaatga
    37261 gaaacgatcg ttccaaaaaa ttgaggtgcc atagccaaat aatagacacg gttaccgttt
    37321 aattgatagt gtccatctaa tcggtcagac aattctttta atgtctgata atgcgttgaa
    37381 tcatttacat catgggattg atagtaaaaa tggcttgcaa aggtagttgc ttcttcatct
    37441 gaaggattca atgcacggat tgtttctttg acaacttcac ggtagtgctc atcgctccag
    37501 ggacggcgag cggttccgat tacagcaaaa tgctcgccta aatttccttt tctgtaaagg
    37561 cggaacaaag acgggtacaa tttacgctgg gctaggtcac ctgtaccgcc gaatatcgta
    37621 aacaatacat ttttttcatt agacatgtgg atacactcct ttatatttta tcgacaaaaa
    37681 tcgtgctggc cgccttaaag ctgacgggga ttttctttct atcattttgg atagtgatcg
    37741 gtccttcata agctgcgatg tcaacgatct catattcttc gttgatattc aggtcaatag
    37801 tgactaagta atccaacaat tctttttcat caagcactcg tgcgatacgc acttttgtac
    37861 cgacaggata ggactccaat gtttgtcggc gttcttcatg gacaacttcc tcttttttag
    37921 gaatcgatcc tccatgagga caatgttcag gatgattcaa atagttttct aaatgatctg
    37981 caagatgttc cgaagtcaca tgttccaata cttcagcatc atcatggact tcattccagc
    38041 tatagtctaa atgttcaact aaaaaaactt cccataagcg atgtttgcga atcagactgc
    38101 tagctttttg cagtccgctt gcagtcaatt gtacaccttg ataaggggaa tgttctacaa
    38161 gctcttcttt cacaagttta gaaatcattt cactgacaga tgcagctgag acatctagac
    38221 tagatacgat ttgtttattg ttgactttcg ttgtatctcc acctagttct aaaatgattt
    38281 tcaaataatc ttctcgattt ggtgtcatat tcgatcatcc tttcatggta aaaaaccatc
    38341 attagtaagt gtaacaaaaa aaacgttaat tgactatttt tctaaataaa aaacaaagga
    38401 tttttagacg tttttacaac aaaaaacgaa agatgataga gaagactcat tctttcgttt
    38461 ataaaggata tatatataca ttgggaggga tctatttttc agataacaac ttatatgaag
    38521 tgtctgcaga ttcgtcataa agagtttgtt cgggataaga aaagataaga taaaaaccat
    38581 actgatcacg tacgactaaa cgatattcat ctatttctac cacagatgtg ccaagagact
    38641 gaccgttgat caaaaacgtg taatcagatg taatctgtaa ttgttgtttt tgctggctat
    38701 cactaaagaa ccatgtacct tgatactttg agagtccttc tgacttttcc gcttgttttt
    38761 tctttttcaa gtaaataaaa cttgagagaa cggtaatgag tgaaccaaga aaatagatca
    38821 ttttttttat caccaaatca ctcctttatg agaaaataat cgcatagaca tattctttct
    38881 tctttatttc attgtaccga ataaaagcta aaatttctct aagactttcg actggttttt
    38941 ttactgtttt tctacaaatt atgataggat acatagtagg aagaaaggtg acataatttg
    39001 aagtatcagc aagtggaaat agcttttttt attgaccgcc cgaatcggtt tatagcacat
    39061 tgtatgaaaa aagacggaga aataattatt gcgcatgtca aaaatacagg tagaggaaaa
    39121 gaggtcctga taccaggcgc tgaagtggct attgtatttg ctcctggacc taaacggaaa
    39181 accgcctatg atctgattgc tgtaaaaaag aagggtgact ggtttaatat cgatagtcaa
    39241 ttgcctaatc ggctggcaat tgatggtatt ttagacggca cgatcctttt accaggatta
    39301 actggtccat ttacggtata caagcgtgaa gtaacttatc ttcactccaa atttgatatt
    39361 tatttagaga cggtggtcgg acaaaaagca tttgttgaag tcaaaggtat gacacttgaa
    39421 aataaagcga ttggtgcttt tcctgatgcg cccacactca gaggattgaa acatatagga
    39481 gaattaacgt atgccgcaca agatggttat gccgcttatg tcttgtttat tgctcagttc
    39541 gaacatcttc atctggcaac gatccatgaa gaaatgcaac cagcactagc tgatatggtt
    39601 cgacatgcgc agcaaagcgg agttcaaatc ctcgcctata attgtcaagt atcggcagat
    39661 gaggtaactg tcaagcaggc cattccattt gatgtagata tcccttttga agatcctata
    39721 caatagaaag aaggtaaaat aatgatcaac ttaggtatta tcggaacaaa ttggattact
    39781 catcaatttg tccaagcagc tttaagcaca aaaaaatatg aattgacagc ggtatattct
    39841 agacatttgg aaaaagcaca ggaatttggt tctcattatg aaggggatat cgaattcgca
    39901 acagatttga agactttttt caatattact catatggaca ccatttatat tgcttctccg
    39961 aatagtcttc actttagtca agctaagcaa gcaattttgg caggaaaaaa tgtgatagtt
    40021 gaaaagccag ctttttcgac gccaaaagaa atggatgaaa tcatccattt ggcaaatgaa
    40081 cacaatgtgt atttttttga agcagcaaga aatatccatg aatcaggttt taaaaagatt
    40141 gccgattact taccattaaa agacgagata atcggtgcaa atttcagcta tatgaaatat
    40201 tcttcgcgct atgaccaagt attagatgga gaagaaccga atatcttctc gactcatttc
    40261 tcaggtggtg cattgatgga tttaggtgtt taccttgtat atgcagctgt tgcttggttt
    40321 ggaatgccaa aagaagtcca ttatttccca agaaagattt ctactggtgt agatggtctt
    40381 ggatggggga tcttacgtta tgagaatttt gatgtatcga tccagccagg taaaatcggt
    40441 gattcttatt taccgtcaga gatttatttt gactcaggaa cgctagtgat gaatggagtc
    40501 aatgcaattg aacaagcaca atttcatgat cgcgcgcata aagaaatcat tgatttggcg
    40561 gtaacagctg cagaaaatcc aatggcagat gaagcagagg attttgccaa tgtcatggct
    40621 catccgaaag atccagcttg gggattgttg tatgaagagt ggatcgagct agctagaaat
    40681 gtgaaccaag tgatttatga tttaagaaaa aatggcggca tcaaattcga tgcagataat
    40741 gaggaagatt tttaatgaac gaatttgtac aagtatttcc cgaaaattgg cagaaaaaat
    40801 atgaggataa agggtttatc tcaccttctc ttattcaacg agcagttttt cagccactaa
    40861 gtaataaaca aagtatagta gcggtttctc ctacaggttc aggtaaaaca ctggcttatc
    40921 tttggcctct actactaaac gtggaaccag gtgaagcaag tgcgttggtc atttttgctt
    40981 cttctcaaga actagctatt caagtagcag atgttgcacg tgaatgggga aaagacaaag
    41041 agttgaaagt ccaatctttg gtgggcggag ccaacgtcaa acgtcagatt gagggtttga
    41101 aggaaaaacc agaaatcttg attggtacac caggaagaat attggaattg atgaaagcaa
    41161 aaaaaataaa agcacatcaa gtgaaaacaa tggtatttga tgaagcagac cagctatttg
    41221 acgctgggaa cagccaaatc attgatcaaa ttcttcatca agctccaaca gagtatcagc
    41281 tagctttttt ttcagcgaca gcagatcgtt cccttgaaag cattgaaaaa atcactggga
    41341 aaacgtttga gacaattgac gtgacagcag aggacgattc tcgaaaagga ctgagacatt
    41401 actttttacg tgtaccgata cgaaagaaag atgactactt gagacgtctc acgcatatag
    41461 aagattttca tggtctgatt ttctttaatc aactgactga attaggcgta atggaagaaa
    41521 aattgctgta tagaggtgta ccggtaggaa gtctggcctc tgatcagaac aagctactcc
    41581 gaaaagcagc gctagaacaa ttcaaaaaga aaatggtcag agcacttttg accacagatg
    41641 tggcggctag aggtttagat attacaggtc ttccttatgt agtgaatgca gaagttccgt
    41701 taagtgaaga agcttatttg catcgttcgg gaagaactgg tcgaatgggt aatgaaggaa
    41761 tagtaattac actcgtacaa gataataatc taagagattt aaaacggctt ttacgtccat
    41821 caaatataac acttgaagag atttttttat acggaggtaa gctgcaaact gagcagccaa
    41881 ccaaagaaga tgcagctcct gcagtaaata aaaagtatgc gaaaactttt tctggaaagg
    41941 agcagaaccc agaatataac aaagaaaaaa taagaaaaaa agttaaaaat aaatcaaaaa
    42001 aagagagaaa taaaggagca aggcgcaaat aagcgccttt ttatgctatg attatgaaaa
    42061 cgggaacaaa attggttgcc agaggagggg ttatgtgaaa gaacgtcaag aagagatctt
    42121 gcagctattg acagaaaacc aacaaggatt gacagcaagt gatgtagctg agcgcttaac
    42181 tatcgatcga agcaatgcaa gccgatattt aagtgaacta tacaaggccc atcatattgt
    42241 aaaaaccgct ggccgaccag tcgtttactc tttgccaacg gaaaagtcaa aatctgatga
    42301 agtgcatgtc gactcttcta cgcaagtgac ttttgaaaca ctcgttggag aaaatgattc
    42361 tttaaaagtc agcatacagc aggcaaaagc tgcgattctt tatccaccgc gggggttgca
    42421 tacgattatt tttggagaaa ctggtacagg gaagtcgatg tttgctgaat gtatgtatca
    42481 ttttgcaatc gattcagaga tgctttctgc agatgcgcca tttgtttctt tcaattgtgc
    42541 ggattatgct caaaatccgc aactgctctt tggacacatc tttgggatca aaaaaggggc
    42601 ttacacagga gcagctcaag atagtcccgg tctaatagcc aaagcagacg gcggtatatt
    42661 gtttttagat gagatccacc gtttaccgcc ggaagggcag gaaatgctgt ttactttcat
    42721 tgataaaggt atttaccgtc cgcttggaga aagtgcacaa gtacatgaag catctgttca
    42781 gatcattggg gcgacgactg aatcttcaga aagtttctta tctacgttca atcgacggat
    42841 tcctatggca atcaccttgc cgaatcttgc ttcacgttca ctggatgagc gttatgaaat
    42901 catctctctt tttatcaaac aagaagcgaa tcgtttgaat caacgtatcg atatcgaaaa
    42961 agaagcgatt cttgccttca tgctttatga tgcggaagga aatatcggtc aagtgaaacg
    43021 agacttgaaa ctagtctgtg caaaatcttt tttacattac cgtactcatc atgagaaaaa
    43081 gctaatcatt cgaaaagaag aactttccct tcaagtacaa aaaggattac tgaaaatcaa
    43141 ggaaataccg gaacgtctcg accgattcat ggacagcaaa agtcagtatt tgacatttga
    43201 accaggattt gctgatgttg tctggtcaca agatccagaa cgaaacatgc aggtctataa
    43261 cgatattgag gaaaaagtca cttctctttc cgaaaccggt gtggagaaca ttgatctaga
    43321 gaccctcatt tcaaaagatg tggatgctta tttccagaca tatgtcaaag aattgactaa
    43381 aagttcgatt cccaaagatc ttttgccaga tgaaatctgg cggttaacca atcaattgta
    43441 tgatgtggct gaaaaacggt tagaccgtat ttataatgaa aaagctcgtt ttgcttttgc
    43501 tttgcatctg caaagtacat tggatcgtgt gagagaaggt catatgatcg tccacccaga
    43561 tttgaataac gtacggaaaa attttaagag ggaattccag gtagcaatcg atctttcaac
    43621 gatcattgaa gaagaagaac aagttgagat tccttttgat gaaatcggct ttattagtat
    43681 gtttctttct atcaatctag gagaaagtga gcctgttaaa gaaaacaaag tagatgttct
    43741 catattgatg cacggcagac agactgcttc aagcatgttg gagatggcac aagaactatt
    43801 aggaacaaaa gtagggaaag cttttaatat gccactggtt atggaagttc aaacaatgta
    43861 tgaagaagtg ttacaatatg tcaaaaacta tcaagagcat ttgacgaatg gcctactctt
    43921 gttaacagat atgggatcat taaacacctt cagtgatttg atttatgaag agactggcat
    43981 tcggacgaaa gctatcacga tgacaagtac catgattgtg atagaagcga tacggatggc
    44041 aagtgttgga agaagcttag aagatattta tcaaaatatc cagctttcct ttgaatcgat
    44101 cgtacgtgat cagttcaaag cggaaaagtc tgaaacttta cagcgtaaaa aagccgtaat
    44161 cgtgacatgc tttactggtg aaggagtagc tgccaaatta tatcagcgta ttatgccagt
    44221 gattaatcaa aataaggtag aactgatcca gatgcagttt ttagaacgtg aatcattcaa
    44281 aaaacacatc gatgccttat tggaagaata tgagatcaaa gcaattgcgg gaacagttga
    44341 tgttgattat caaaatatcc catttttttc agcctatgac gtttttgatg atgaaaaact
    44401 aaatatttta aagcgtatag ctgatgatga gatcccgaca gctaaaattg tcgattcact
    44461 tgctggatca ttaagcgatg tcttttctat tgatgaactg atccgttcat tgcaaaagac
    44521 agttcagcag atccagaccg atttgtcgat tatcgtggaa cctggagtag atactggaat
    44581 cgttatccat ttagccttca tgttagacgg attgatcaaa caagaaagcc atcggcaatt
    44641 tgagaaactg tctattttct caaaaattta tcgtttggaa atggatatcg ttcgggcaaa
    44701 cttgatggca ctagaacgaa aatatcaggt aaaaattcca gaagatgata tcgcatttct
    44761 tacacaaatg tttcttgaaa ataaaataaa aaaatgaaca cactaatcat actgtttaca
    44821 cactaaacat ctgtgtgcgc actataaaaa agagctaaaa agcatgaaaa cgctatttca
    44881 gctctttttt ttattggcat gcttcttgcg ttatataaaa gtgtaaaaaa ataaaaggag
    44941 tgttaaaaat ggcaaaaaaa acaatcatgt tagtttgttc agcagggatg agtacgagtt
    45001 tattggtcac aaaaatgcaa aaagcagcag aagaaaaagg gatggaagcc gatatttttg
    45061 cggtctctgc ctcagatgca gataataatt tagaatctaa gaatgtcgat gtattattat
    45121 taggtcctca agtacgtttc atgaaagctc aatttgaaca aaaacttgcg cctaaaggta
    45181 ttccattaga tgtcatcaat atgcaagact atggcatgat gaacggagaa aaagttcttg
    45241 cacaagcaga aaatctaatg aaataaatca agtatgcagt tgggggtata acaacgtgga
    45301 agatcaacaa aatctagaag ccatcatggg gttaatcatg tttggcggta atgcaaagag
    45361 cgatgcaatg gaagcgattg cggctgctaa aatcggtgat tttgagttag cggataggaa
    45421 aatcgctgat gcagaagaat cacttataca agcgcatcat gcacaaacag acatgttgac
    45481 acaagaagca caaggaaatc atatgcaagt gacattattg actgttcaca gtcaagatca
    45541 cttaatgaca tctattgcgt ttactgattt agcaaaagaa atcatcgaac tttatcgtcg
    45601 cgtatacagc gaataaatac aacttctttt ttgaacggaa gaagaaaaat ttttgaacgg
    45661 attttttatt ttgttataag gagaactctc tttatataaa aaacttatag gaggaagtaa
    45721 aaaatgaacg gactatcaag ttggatggag aaatacatcc tgccggttgc cgcaaaaatc
    45781 ggagcgcaaa aacatttagt tgctttgcgt gatgctttta tcggcatact gcccgcaaca
    45841 atggcgggat cagtcgctgt aatgatcaat gcaattattc gtgatttacc gcaacagttt
    45901 aacagtacct atgctgacag tttagcagca gacaaaggaa tctttgccgt attgaatgtc
    45961 atcatcggaa tcaatggttt tgtctggaac ggtacactag caatcgccgg attgatcttt
    46021 gccttttctt gggggtataa tcttgcccga gcttatggag taaatgatct agctgggggg
    46081 atcgttggtt tagctacctt gattcaaggt atttcctttg gtccagcatt atcagcagca
    46141 ttagatgtga atgttccttc aaatgtcgca gatgcaatca atagttctga gattggtgca
    46201 acgattgcag atggcacact gtcagttgga ttatggggtt ggctaaaact agatcatttg
    46261 aatggaaacg cttattttac agttatgatc atgggagcta tttcagtcat tattttttgt
    46321 aaattgatgc ttgccaacat tacgatcaaa atgcctgatt cagtaccacc agcagtatct
    46381 aaagcatttg cagccatttt accagcgacg atcgctttat acgtcattgc tattatcaat
    46441 tatatcgtag gtcgtgtagc tgatgggcaa ctgatcattg atttggtgca aaaatatatt
    46501 gcagaaccat tcctaggatt atctcaaggg ttgggagcag tattgatcgt cacagtattt
    46561 gttcaagtat tctggttctt tggtattcat ggatcaaatg tattggcacc agtgctagag
    46621 ggaatctggg gacaagcaca actaatcaat attgatattt tccaaaaagg ttataacgga
    46681 aaaactggta caccagccgt gttagatgca atcgaagatg gaaaagctta tatgtgggtc
    46741 cgcggatcgt tcgatgcttt tgcatggttt ggcggatcag gcggaacgat cgtcttactg
    46801 atagctatct tgattttctc taaacgtgca gattatctaa cagtagctaa attatccatt
    46861 gggcctggta tcttcaatat caacgaacca gtgatgtttg gtctgccaat cgtattgaat
    46921 gcaatcatgt ttatcccatt cttgattgct ccagtagtag caacagcaat tggatggaca
    46981 gctacttatc ttggcctagt cgctccggta tcgcagcaag tcacatgggt agtaccgccg
    47041 ttcttattgt cattcttagc aacaggagcg gattggcggg caccgatcgt aacattagta
    47101 tgtatggtcg tgactttctt gatttgggca ccatttgtca tctctgcaaa caaaatggac
    47161 cctgccgcag gcgaacaaca ataagatgaa cgtgaggagc gggacgcttt aagtttcgtt
    47221 cctcataatg aattttcgag gaggaactga aatgattaca attgccagtg caatgcaaca
    47281 agatgaaatc aaagaattgt tagaaggata caatgaagaa ggaatgactt ttacatttaa
    47341 agaaaaacaa ggaatcaagc tgttctttga aacaagtgca gaagatccgg aagttgcggc
    47401 taaaaaagca aaggaactaa tcaaagcaca gccttgggga acagtccttt attttcaatc
    47461 aacagctgtt tagaaaatgg tttaaaaaga taggatggtt gagatgataa aaaacggttt
    47521 atttttatgt agtattggtt ctttgatcgt gttgtatagt ttgaatccac attcgcaaag
    47581 gtatgatttg tatagtatgg tcacaggtct ttttctaaca ggattaggaa tctatttctt
    47641 catgaagggg aaaaaaagag aagaagcaaa ggagaaaaat aactaatgga aatcacgaaa
    47701 aaattaaata ttccagcatc gtttttttac gataaagtta tggactctgt ccttttcgat
    47761 gtacgtaaag cgaccggcaa atcagtaact agaaaacagc tgaaaaattt ggagtacgtc
    47821 aaacaattct cgcaaaataa ccgggctcgc ataaaaatcg aagaagtcat tgaaaaccag
    47881 tcttataagt ttcgtacatc gacaacgaag aatgaattcg tcgttcatta tcaaatcaaa
    47941 ccattggacg attcgacctg tgaggttcat tacacagaaa aaatggagtc ttatggcatg
    48001 cttcaaaagt tgaatgatat gcttttagga acaatcttga tgtattttaa acggagacgt
    48061 ttcaaaaaaa tgctagatat gatcgaagca acgtattaaa tcaatgaaac atggtactat
    48121 ttagttattg aaaaatgaag taagctagaa ggaatttttc ataggaagat agatctttcc
    48181 tgcgaatcct cactagcttt tttgtaggaa ggggcagcaa aatgacaaac aaagtaagag
    48241 agctaggtgt atcttcgatt gaggaaatgt ttgatttagc cacttatgca tttaatgggg
    48301 cagatacacc tgaaaggcga gaacgttttc gtacacttgc ggagaattct tggaattacg
    48361 gattttttga tgaggaggat cggctaacaa gtcaagtcat ggcgacccct tttccagtga
    48421 atttttacgg acaagagtac ctaatggccg gcattggcta tgttgcttct tatccagaag
    48481 caaggggaca aggcgggatc aatcagatca tggaaaagat ccttgaagat tgccgtgatc
    48541 gtaaagtaag cttatcttat ctggcgccat tctcctatcc cttctatcgg cgctatggct
    48601 atgaacaatc atttgataaa atcagttatc aactaaacag tcgtgatgtg ccgtttatca
    48661 aaaaaaaatc tggaaaaata aagcgtatgg attttgaaga tgcaaaagct gcaatcagaa
    48721 gaatttatga acagatgccg gaaaaccaac gagctggtct gaagcgcgaa gactggtggt
    48781 atacgtataa attcaagcaa aaaggcaatt accagtttgc tttgcatttt gatgataatg
    48841 aggaagcaac gggatatatc gtttatcagc ttgcaacgtc ttcctttatt attgcggagt
    48901 ggggattttt ggatcatgat gctttttgca gccttgttcg cttcgtaagt tctcacaatg
    48961 gtgcttttga aacatttgaa tattcatgcg gctacggcgg taatgatcaa aattatctat
    49021 tagaaaatcc atttgcttct gtaatgatca caccttatat gatggcgagg atcgtagata
    49081 tttctttgtt tttggaaaat tatccattta aaaagagaca agctgcattt gccctacaaa
    49141 tagaagaaga tcattatgcg aaatggaatg aaggaatcgt ggaagtaaca atcgaaaatg
    49201 gaaaaaatca tctctcaaaa gtagaacaaa caaaactccc aatcgttcga ggaagtatcc
    49261 aacatatcac gcagctatta ttagggtatc gtaaagtgga agagttggta ttccaagaaa
    49321 gaatacaggt gacaaaagaa tatgaagaaa cgttaagctc aatcttgcca cagcaacagc
    49381 caattcttaa tgactatttt tgataaaaaa cgggtgatga caatattttt gtcaccaccc
    49441 ttttgttttt catttggcat ttgaaggaaa atgttaaagt aaatcctctt ttttcgtaaa
    49501 tttgaatttt ttgttttctt ttgttttagg gttttctttt ttgtggtctt ggattgcttc
    49561 tttcgtaatc ttttcccgat tttttttaga tgaatctaag cgttctgcgt atactttttc
    49621 ttttggctta tcattcattg gaaaggacct ccctacatta tttcattcta cctctagttt
    49681 atcatctgtt aaggactagt ggaaacgttt tgcctaactt ataagctgat ttttttagca
    49741 gataagcttg caagtaaaaa acaaacaagc tacaattgat ttattgaaat tgttcctgta
    49801 gctcagtagg atagagcaat cgcctcctaa gcgataggtc gctggttcaa ttccagtcag
    49861 gaacgtataa ataacccctg tatcctttgc ggtacagggg tttgttttgt atttgtgcca
    49921 aaaatgtgcc aaactagtta tcatttttat ataaactatc gaacacagta ttgatttttt
    49981 ctttatcctg atcttcaagc tctcgaacaa tatgaagata agtagacatt gtagtttcta
    50041 aactgctatg tcctaaacgt ttcgatacgc taagtatatt tactccttga taaagtaaaa
    50101 tagatgcatg tgtatgcctt agtccatgca aagtaaattg cttttcaatc cctaattctt
    50161 ttaatttttt gcgcaaatat ttgctgacgg cgtttgatga aactaatcca ttttttaagt
    50221 tgaaaaaaac aaaattatga gggtttttga tttcaaaatt ctcgaataat tctttttgat
    50281 tgattttaaa cttttttaag agatcaatcg tatgtttatc aatggaaatt ttcctatttg
    50341 aagtttcgtt ttttgttttt ccccattcat ttaatttata attccatgtt ttattaatag
    50401 tgatgatttg atcttcgaaa tcgatatctt cccatgttaa tcctagaagt tcagcaaatc
    50461 gcattccagt ggcaccagca actaacacga gcataggaga agagtactgc gcagaaaggt
    50521 tttcttctac gagtttcatt aaagatttaa attgatcata atctaaatat ttatcttctt
    50581 ttggcttcaa tgaatctttt cctttgatta ctgcttttct agttggatca aacggaatta
    50641 atccttcttc tacggcatct ttcaacgacg ctctaatatg gttattaaac ttaataacgg
    50701 tagattttac atggtcttta gcatacttat ttaaaaagcg ttgatatccc actctatcta
    50761 aatcagaaat caataccgct ggcatatatt tctttatgtt cattaacgta tcttcgtatt
    50821 ttctataagt gataggagat actgttcctt ctttataaag ttgcatccaa tcctcaaagt
    50881 aatctgataa taatagattt ttgcgatccc ctttaaggcc tttgttcaag ttatattcta
    50941 attcgttggc ggcatctttt gcttctcctt ttgtcttaaa tcctgatttt cttatcttcg
    51001 tgtattttcc gtcatctttt ttgtatgaaa tttcgtattg ccaactattt ccacgtttga
    51061 ctaatcttgc cataattgaa tcagctctct ttctctgata caataggaac tataaagaag
    51121 cccattgttt gggttttatt attttttgca catccttttt gcttgccggc gcaaggatgt
    51181 gctatttgtc gttttaatgg tataattgga tgtgtattag atcatccaat cagaggtgtt
    51241 tattatgatt aaagtgattt ttaaaagaat aactgaactt ccagagttta gattatttgt
    51301 tgattattgg tatgtctggt tttttattgt tgcattgctg ataggtctag atgtcatatt
    51361 agaaagaaaa cttaagtaag agttgagtta ataaattgaa ttcctaaatg agatattttg
    51421 tagcccatat tttgtttgcc gacatcacct aatactatgg agctaatcgt tttttctaac
    51481 agaccaaaat cagataatct atttaagcta ccatctatta gctcttgtga atagccagac
    51541 atgctgatat ttccagtgaa attttcgttt ccacgactca ttaagcatgc taacaattga
    51601 atctcaatcg atgttaattg attcagtaca agaagataat actcctcgtt atcccatgaa
    51661 gcgttgttaa ttcgaagtaa acaattctta taaagatttt tatacatatc tattttattt
    51721 tgtgcctttg atttttctac ctcgtcattt atcccttcga atattccaat gagttggtcc
    51781 ttgttctcta agttgaaaac atcttctgga atttgatcgc gaatgttttc taatgattcg
    51841 tttaaatttt tgtagaattt ctcgatgcgt ttgaaacgct tctcgttttt agacccaaaa
    51901 taaagtgact gcacaacgcc accaatataa ggaattgctc caataccgcc ttcaactatt
    51961 aattcaagat attctttttt ggttagtttt tcattgctat tatccatagt tacctcctat
    52021 tttgtcattt cctttacaag cattgtgact cctgatggca ctcttttgta taggttttat
    52081 tattgtgcac aatctaactc ttagtggggc gtggttcatt tattttttac ttactttact
    52141 attaatcata tcccattgag cataggattt ttctatgttc gatcctcttg aactaatttc
    52201 tcctgtaaaa gttactttat cgccctttgt aaaggtatca gcattgttta agtccttcgc
    52261 aaaaattaca tatgaaactt taggtgttgc agaaatatca ttccaacttt tatcatcgta
    52321 ctctttgtct gctataatgg ctattctagt agtcattgat tcgataacag ttccttcaaa
    52381 agtatatgtt ttaccactaa tatatttagt aaaagctgta ttttgctcag tagctggagt
    52441 aatagaataa taactatcta agaaagcacc ccagtcatga ttatttgtct ctaaaaattc
    52501 ttcaaattga gttaggcttt tattgctgct gttagatgta gtagtttgag tatcagatga
    52561 tgagctttcc tgtttgttat tgtcatttgc ctcgtttgat gaggaaagcg agaaacctac
    52621 ggtagtaaaa ataatcccga taataaggag aatcataggt gttttcttat ttgttttttt
    52681 gataaatctt acaattaaca tgattaaacc tataataaat aaaacaatac cagtaaaacc
    52741 aaatataccg tccattaata gatcctcttt ctcttttgat attgaatatt ataaaccaag
    52801 caattctttt ttcttaaaat cgaattcttc ttgattaata atcccttcat ctaacaactc
    52861 tttatacttt ttaatttcat ctgcacttga aatttgtgtt tctttggaat tattatctaa
    52921 tatagattgt tgcttctcta tatatttttt taaattttcc atttcattcc aatatttttt
    52981 tgtaaacata acggtgtttt catctttcgt ggccgctaat atacctccgc gactttcgtt
    53041 tccgcctagc aaggtaaatt gtatataacc attactcata cctggttttt ttaattgtac
    53101 ggctgatata tttttaaagg ggatagtttt ttctccttta ataccttgat taactaaatt
    53161 tataaatcct tttctaataa ttgaaataga atcatcagtt actttaatca aggtttttcc
    53221 tggagatttt attaaaatct ctttttccat tttttcttcc tcatttctgt gatatgattt
    53281 tttttgtaga agctcatata tgaggaaaga gcccgtgttg cagcacgggc ttttttaatg
    53341 ttttgctgaa atcggttttt ttaataattc ttgatgtatt catacatatt gccttgcgta
    53401 agaatatttt ttcttaaaat agcattggca gacagcataa caagtgtatc tgagcttatt
    53461 ctgaatagaa taatactcca taaatttttc gagattaaac tgagattcat cagtcagttc
    53521 gttctcaata tagatattta ataaaattag aatagctatt ttatctgctt ctgtttcaaa
    53581 ttttgagtga aaagttgtag aagtatcgta caaaactgaa aattcaaaat gagaggcgct
    53641 gaaatgtgca agttcatgag ataaatgaaa ggcttctgca gtttcaccgt atagattttc
    53701 attcaaaaaa atgattctgg gttttggata gtagaaacca ggctctttca tctccatata
    53761 aactactttc aaattgtatt cgctcaacat ttctttcaat ttcaaataca tacaaaccat
    53821 cactccaact attcattttc ctctaaagct ttagcaattg caatcgcttt acgcattgtc
    53881 tccttagata tttcttttcc atcaaaagaa aaaacagtat cgtcttctga taaatccaca
    53941 tgtttagggg tttctctttc ttctctacct agaaggtagt ctacagagac atcgaaatag
    54001 tcagcaatcc gacttaattc atctgaatta ggagtattat ttttccactt tgctagataa
    54061 ccgttagaat atccaaaatt aatttctaac tgccgtatag atatcttttt cctcttcgcc
    54121 aagtctttta ttatttcata ggtattcatc gataaaacaa cctttctaaa tgtttacaaa
    54181 aaaagtttag aaaaatacgc agaaataatt tgactaattc tgagtaatga gctatactat
    54241 atcttgtaaa cagatttaac aactaaaaag acaacaaaaa acactattga tatgtaaatg
    54301 cagaccgcca agaaagcttt aaaatcaatg tttttatgtc ttatttaatt atgctcttag
    54361 tatagagtat tactcagata ctgtcaattg aatttagaaa aaagttgtta aatttgttta
    54421 cgaatataaa agaaaggaat gagaaatatg aacacaccgc aaatttttaa tttcgaacaa
    54481 aacgaagttc ggacaatttt agtaaatgat gaaccatatt ttgtaggcaa agacgttgcg
    54541 agtgttttgg gttattcaaa cactaaagat gctttgtcgc ggcatgtaga tttggaagat
    54601 aagatggggt cgcgaatcac gacctcaggt caatcaagag agatgacaat catcaacgaa
    54661 tctggtttgt acagtttaat cttaaaatca aaacttccct ctgccaaaaa atttaaacgt
    54721 tgggtaacaa gtgaagtgtt gccagcaatt agaaaacatg gaggttatct aactccagaa
    54781 aaagtagaag aagctttgct taatccagat acaatcattc aattagcaac tcaactaaaa
    54841 gaagaaagaa ctggaagatt aatcgcagaa caaaagattg cagagtacga acctaaaatc
    54901 tcctatttag atagcatatt atcttctaca gattcagtaa caattagtca gattgcagca
    54961 gattatggga tgtctccaca acagatgaat aaattacttc ataaactagg tattcagaaa
    55021 aaagtcggta accaatggtt attgtgcaaa aaacacatga accaaggata cacaaaatct
    55081 catacaactg agatcccgaa agccgatggt ggcactaaaa ttgtaatgaa taccaaatgg
    55141 acacagaaag ggcgtctatt tatctacgaa ttactaaaaa aagaaggata ttaccctcaa
    55201 atggatttag aggaaattgg ttagaaagga gttttagtat gactgacatt gcagaaatca
    55261 ctcaacgaga tagagaaaaa atcaaagaat atgtcgaaag ttcaaagttc ttaacttaca
    55321 ccatgcttgc tgaaagattt ggaattagca aaagctactt atctttaatt ttaaacggta
    55381 aaaagacttc tgcagaagca aacagaatta tagattcgat tatcactatg tacgaattgt
    55441 aaagggagga acagctaatg caatatctag aagcaaaaat cccaattcca gaaggctatg
    55501 taattatctc ccaagtggat tatgaggagt taaaaaaagc tgatgatact ggtagatgga
    55561 tgacgttgcc agaagtgcta gaacggatta acagaaaata tgattggttt acttctagag
    55621 ttttaaagaa cccaagatat agaaatatta tcgatatcga gaaaaacaaa aatggatttg
    55681 tctattatcc agttgaagga agagacacat atctattttt aagaagtaaa acacttgaat
    55741 ttttagaaac aaatttttcg gaaatcttaa ggaggcaagc ggatgggaaa atttaataga
    55801 gcgctagtgt tcagcgcacc gctaatcatc tacgctttag gactttgggg aagcaggcaa
    55861 gcgttgatag gaacgattgt ttacatggtt tggattttta tggggcttga tgaagctgaa
    55921 gctgagtaca gagcgaaaaa gccaaccgag ggggctgact aaaaatgaat agaaaagaga
    55981 aactagaatg tatattacta ttactcagtt taattctttc actaatttct ctattgggaa
    56041 gtttgtattt ttgatatcaa caattttgtg gatttgggtt ctatttagag taaatatcat
    56101 ttttgtttgg tgattaagga gtttattaaa attaattttc cctacttcaa atgcaaggta
    56161 acaagatata gcacttttag gaggaatatt tagcggtaca gcttcagagt atattctatt
    56221 atcatcgact gatcctcctt tagcaattaa tttcttaaat ggagtagctg accatctgcg
    56281 gtttaagtta gcggatgaaa gttctaaatt aactaacata gctggttctg ttgaatagtt
    56341 agaaatgatt acttttgtat aaatcatatc tgcagcgaag taagaagcat taagttcaac
    56401 ttctagttga gggcgcattc cttttatttt tatccctgtt aaaaacgtac tcaatataaa
    56461 acttattatt gacatccatt gaaaaattgt taagtgaaaa aaattcaaaa taaacaccac
    56521 cagtttttaa ctaaattata ccaaaaagga gagaagaaat aatgcaagaa ttagtaattt
    56581 tgaaaaataa agaagctgtg actacgagct tacaagtggc agaaagcttc gagaaaaaac
    56641 atcaacatgt tttaagagat attgatgcac taaaaaaaga tgtgtccaat tttggacaga
    56701 tgtttgtaga aggtaatgaa ccagattcat atggcagaaa tcgacgagtt ttcttcatta
    56761 gtagagatgg atttttcttg ctggctatgg ggtttacagg aaagaaagct atctacttca
    56821 aacaaaaata cattgaagca ttcaacgaaa tggaagatgt tattcgcaag aatactgttc
    56881 ctcaaacaat tgaagatatg atgatctatc aattagaaga aatgaaagat gttaaaaaag
    56941 atgtttccat gcttaaagat actatgcgaa ttagcggaca acaagagttt gaaattaagc
    57001 aaaaaggaaa tatgaaagtt atgggagttc tagggggaaa agaaagccga gcttatgaag
    57061 aaatcagcaa aaaagtattc tcaaaatttt ggtctgaatt taaacgtacc ttttcaatcc
    57121 caagatatgg cgagttacct cgtaagagat tcgatgatgc tgtttcattt attgaaatgt
    57181 ggttgccaga aactgcgatc cgtatggaaa ttgatcaact gaacagacaa cagagacttt
    57241 ttggtgatga caatgaatag agctgaagcg ctaagaatag ggacggcaat tgctaattgc
    57301 tggtggaaat actataaacc aatcatccta agccaacaac atattgacaa gcaaaaagca
    57361 tggcaacaaa taaaaaagtg actccgccgg caagcaaaga gtcacaaaga aaacacatca
    57421 taaggagatt ttagcatatg gaaaaagaac tttccactct agatcaatat ttgattgatc
    57481 ctgattgggg caagccgaaa attgaggaaa caagtggtcg aaaaatcaga cgaaatcttt
    57541 tgacgaatga agaactagct tgggatcaag atgatttagg caaccatgta actatttggg
    57601 atcatgttta tcttatccat ctatcgaagc attcgaataa acctgaatat atttacgtca
    57661 tcgaagatgg cttgattgat gcgctagaag agtacgacag agataacttg attgatatct
    57721 cttattacgg accaggtaag aaatacattg ctgaaatgga ggcagaattt gatgagtgaa
    57781 atcaaaggga caacgaactt tgaaaaactt tttagtcgta agttaaataa aattctcaag
    57841 aaaaaaggaa attttgatta tttatcttgg gctcacgcgt gggagattat gaaaaagaat
    57901 gatccacagg caacggtaac tattaatgag tacaaacact acagggttgt ttctggaact
    57961 catcaagact ttcttgttga ggaatataaa ccttttctta tggatgaaac tgggacttat
    58021 gtatctgtct cagtaacggt taaaggacac acggaaaccg aattatttcc tgttttagat
    58081 tatcgaaacc aaccagttgt taaaccaaat gcaatgcaaa tcaataactc attgaagcga
    58141 tgctttgtga aagcattggc tctacacgga ctgggattat atgtatttca aggggaagat
    58201 attccaacac cacctagaat cgatacaaag aaattaaaca tgctagagac gattctagaa
    58261 gctttcaatg agcagatggg taaagatatg accaaaacct taatcgaata tgttaatgag
    58321 cagacagata aattagggct cttagctgat aacgttgaaa ctattgaaca gttaagctat
    58381 gagcaatgtg ccttgatgga gcgagcaata gcagctaaga gaaaagaatt agataagaag
    58441 tgatatgagt gtttaaacca ttaatcgatt catattcagc ggttctgaaa aagttcaaag
    58501 gaaaagacat aagcgcaacc atcaatgagg aagtgaacat tgatcgacta aagacgatgt
    58561 atgacggcta cgatggtgat cgagtcattg aaattcgttt tattgatcct agacgtttca
    58621 ccgtacagca acgaaacttc atctatgcgc tgataggcga tatttttatc gatacaggca
    58681 tgccaacgga cttctggaag gaattcttct actttcgttt tgaaggtgtc acagggcgca
    58741 aaataagcct gaaagacgaa tcgaatacaa ctgtgagtga tgccaatgtc ttagcaaata
    58801 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
    58861 ctgcgaatca agaatattac ttctacaaat gcatcacaaa aagagtctgc tgcatctgtg
    58921 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
    58981 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
    59041 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
    59101 agaaaacaat caaaaagtta aatattggag gggaaaaatg acagaacatc gaagttatta
    59161 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
    59221 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
    59281 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
    59341 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
    59401 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
    59461 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
    59521 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
    59581 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
    59641 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
    59701 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
    59761 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
    59821 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
    59881 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
    59941 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
    60001 ggaattgaag gaattaagaa tgatcgttcg agttggagta aactcagata ctatttcgca
    60061 ctctatatcg atggaacgca attaagaaat ttagcatata ccaaattact attcattgat
    60121 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaaac aagatggacc
    60181 taaagacatt tacagcacag atcgaattaa tgcatcaaga agctttaaga caaagtgtgt
    60241 cgtacgaaga caagtggctc aacacatttc atggcggacg tgagagcgca cttgatcaag
    60301 tactcaaatt actgaaagga gaatgtcggg atggataaga aagcggcaat gcagcgaatt
    60361 atcgaattga cttattcaga agattggcag gaagataaaa aaatagttgc agaagtccaa
    60421 aagctcggta aatcaatgtg gactgaaaag cccaaacgga gaacgccgag aaagattgca
    60481 atctggcatg gtgatcgaat tctagtaacg ggtaccgcta aacagttatc tgaaattact
    60541 ggtctgagca aaaatattat ttgggataga gctaggagct tatggattga ttcaaaagga
    60601 agacagttta ggtatgtgga ggagaaataa tggacgaact aatcgcgaaa gtagagcagt
    60661 gggcaaaaga taagggattg gatcaagcgg atttcagcaa gcaaatgtta aaaacggtcg
    60721 aagaaatcgg ggaagttgcc gcttctctag ctagaaaaga tgaacatggt ttaagagacg
    60781 gaattggcga tgtagtagta accttgatca tcttggcaat gcaaaatgat atggatttat
    60841 acgagtgctt ggattttgct tatgacgaaa tcaaaggtcg gactggaaag atggtcaatg
    60901 gtgttttcgt taagtccagt gacctgaaat aattccatta atggaagaaa taactgaata
    60961 ggaggataga catgattgat gcaaaacaag tgcaaaaaca gaaagatgga atgttgatgt
    61021 ttgaagcata tgttctacca tttcttaatc aatttgaagt tttggaatgc tctgccagcg
    61081 gagaagaatt ggagtatgtt gtaattcgtg aaaccaaaga aaacgttcag aagctaaacg
    61141 aatttctttg cactattaac tgttgggata tgatagctcc aggattttta tgtccagcta
    61201 tgggagaatt tttggaatat tgtcgtcttg aagatgcagg cactttggat ttagcttatc
    61261 tggtatacaa ctacctaaac atcaacacag atcatttgtg gtttggcact gccgaaagaa
    61321 aatgggtagt ccgctaacca ccaatatcaa tgaaaaaagg cggtttcctg aaagtggaat
    61381 tgccgccatt gctgtaaggt tctttacgcg actcaaggtc acagaaatat aaatgcaaat
    61441 ctcttttgaa gtgatccaaa gctctcggaa aatggaagtt ttttgtttca aagaataaat
    61501 gagatttgcg tcaagaacct gagagttatt tccaaaaaag cttccaaaga accgaatgag
    61561 ctaaggacat catgtgtaaa cacataatat agctctcctt tcacggctaa tcataacatg
    61621 aaaaatattc gatgtcagta ggaaaaaagg tgaattatga aaagaatatt aaattatccc
    61681 ggcagtaaca gattctgcaa ttaaactccg aagctgatgt atcagtatca actgtagaat
    61741 attatgagcg tggagaaagt caagcagtca caaatgtaaa aatcgataac gtcaacggtc
    61801 ttatctgttt agatgaaggc gatagtatga atgaaattgc tgcagaccat gaattatcag
    61861 aaactgcttt aattgatcaa atcgaattag ctagacaata ctttgttcca tatgcagata
    61921 cctggtccaa gcataagaat gacatcattt ttccagagga acaatgagag caactgatcc
    61981 agtaattatc cttgaggaag ccaaatttat ttggactcac gaagagatag agcaagcacg
    62041 cttgctcttt tctcaaggag ttaagccgag caaagtagct gaaataatgg gtcaaaagat
    62101 tcttgatgtc ggattgcttt tgctccatct agcagaaaaa atttgatttg aggtgaaaat
    62161 gatgattcaa cttgcaggca tccaaacagg gaaaatttat ttttctggag aaagcaaaag
    62221 cgaagccaac caatggctat tgaaaactta cacgaacaat aagaagctaa ggaaaaaata
    62281 tcctgatttg ttgctaaagg atgatcagat tatgccggaa ccgatgattt tgacccgtaa
    62341 ggagggaagt tgaaactaaa aaaccagccg tgtgggctgg agttgctatt tctttttaat
    62401 aatgagtttt tctaaattta attcattctc acgagtttgc gtccaataat cagaataata
    62461 ttttaattgt ttaagagtag gaatgtcgtc ttcggtaaaa ttagaaaata attctggaga
    62521 attatctttt tttaattgtt ctaatcctga ttctttaaaa cgttgttcaa cgagttttac
    62581 taaattgttg atattcatga taaatcacct ttttaaattt aatttcgatt tgattaatcg
    62641 atacaagtat tatatcaaat aaaaaagctg atttaggggg tcttacaaaa acactaaaat
    62701 tttttcaaaa agaggaggaa cggtggtgaa agtcagagaa caattcaaga tagtgagcga
    62761 cgaaggtaat gtttacaaaa taaagatcgt taatattaat gaatatcgag aagcagatca
    62821 caaatatttg gttgacttat acatgaacga taaatatttg ggagaaggat tttgtggaga
    62881 ttcacttttt gatgatgagc gaattttgaa ggaggaaaca gaatgatcca cgaactaaaa
    62941 atactaccag aatattttga agcagtgacg agcggacgca aacaattcga gatccgcaag
    63001 aatgatcgtg acttcaaagt tggtgatcag ttaatactac gtgaatataa aaaatacatt
    63061 aaacatgaat tacctggact aacgagacca acatttagtt acactggtga ctcatacaaa
    63121 gcagagatca cttatattac tgattacatg cagaagtccg gatatgtagt gttggggatt
    63181 aaaggggtga attaaatgaa agtatacatc ttagctatca cagagggcac atggatgttc
    63241 ccagtcggtt ccggaaaaat ctataaatca aaaacagctg cttataaagc atttgaaaaa
    63301 tacaaaaaag aaaacggtgg cggcactaac gcaaagattc tagtggctga taactggcat
    63361 gaagaaggtg aacgtaattg atttacctgt tcattttcgt ggtactaact atttttatgc
    63421 tagtaatctt attcaaaaaa agatgagtaa agacgaacga aaaattctgg ccaactttta
    63481 tttagtatta aaaatcgaac aaaatgtgtt ttcggaaagg agaattgtat gggaaaaaca
    63541 aaatcaaaga ttaagaagaa aaaacgacgt ttggaacaaa aggcaatcca gaatggaacg
    63601 gctaagaaaa aataaaaaag ctaccccttt cgagatagca gcgcaatact attctaccat
    63661 aaggggtggc gtttgtgaga tttcattggt taaaagatta ccaagaacta gatgagcaga
    63721 ttctttactt aaagtggaat cttaataaaa gtaagcttga attgaatcga tgggtctgtg
    63781 gtgatttagc aaacgtccgc atcgaaaaga attcgagaac ttcttcttta gaagagaata
    63841 tccaaaagat agaaaatgaa ttggaattgt tgatcgagca aaaagaagaa atgttattgc
    63901 tgatagatag cttttctggt atcgataatc aaattgttaa gatgaaatat gttgatcaaa
    63961 tgagcttaga agatattgct gaatctgttg ggtatagctc atcgtatgtc agacagcgac
    64021 atgcagaaat tagaaagaca ttgaactttt tagatgagta tgaacacaga caagctgacc
    64081 gattgaaaaa agaaaacgaa attgattttt acaatagcga gaagtacaaa gaacaactct
    64141 ctttattcta aaattacact gttcactaaa tgttcggtca ttgtagctat gtaaacattg
    64201 ttagcagcat gatattctat tagtgtcaaa aaaatatgaa agagccaaga tatcccaacc
    64261 gttttattaa ttggtatctg tggctctttt attcttttaa ttagtagttg gcataaagtt
    64321 atttccattt ataaattgat cagctaaatc agcatcataa tcagccgcga taaattgcgc
    64381 tgctgattta ataaatgcat ccatatcatg aacatcttta ttagtatgtc tacgaacata
    64441 gtgcgtttca tcattaccaa tccaagtagc agctttggct aatctttgga gtttaggaaa
    64501 atcattcaaa taattttcaa ttgttttacc taataacatt ttttttattt tatcttcatc
    64561 ctctgaattt ttagatattg cataatcttt aattagaaat tctagagctt ttctataacc
    64621 aactccagca atttgttcca attgctcaga tttagcttgt gtggcttgag tataaatatc
    64681 aacaaatgta ggagaaactt tttcaatatt ttctggtaaa tctattttaa tcggcggacg
    64741 gtaataatct ttaattaaaa tattttcaga atatccttca tcttcattaa acgtatttat
    64801 aaattgtaaa ctataaaatt tgttgcactc atcatttata cactgtacta atacgccaaa
    64861 gattctatca ttatcgaacg cactatatga tgaaacccca aaatacacat acggagtcat
    64921 tatatttccg caatgcggac aactatttgg caaataaatt gtctctgtat tttgacgatc
    64981 acgatactta atattaactt cttgaagcat taaaacacct cgcatctttt ttataaatca
    65041 taccacaaaa tatctgatag gagagtaaac aaatgaatga ttttcatgaa gcggtactta
    65101 gtattgaagt agaatcaagt ttggctaaag tttataagaa agcaattgaa gcagagaata
    65161 gtccgtacag agaaaattgg aatggtaatc atgcacatgt gcaagtggag aatgatgatt
    65221 acagaactgg aatgaatacg ttagttattt ctttattgtc acacactcta cccaatctgc
    65281 aggaaacgat tgaatggtat gagcgaatgg gcgcaaaagt aatccgtaca aattacaaag
    65341 gagagaatta aaatggcaat gatcaaaatt agaacatcca ttagtggaac agagtattgg
    65401 gattcagaaa agaaaaggac agtagttgtt ccgaaagatc aagaaccaaa ttttgaagta
    65461 aaagaagaaa aaggaatttt gagtgatggc aaatcatttg ttgctgtcaa tggcgaactc
    65521 atagctcata gcagtgaaat tcttgatagc gatggaaaca ctgctgctaa ttttgatgga
    65581 gacgaagcta ctaatgatca gtccactgaa gatacggatg aactggacaa tatgactgca
    65641 aaagaattgc gtgcatatgc taagaaacat ggtattgata ttcctggtgc tatccgtgca
    65701 aaaggcgata tcataaatct tattcgtgaa gtagaatgaa atactgtcag tttgacggat
    65761 gcacgaacaa aatagcaaag ggtatctact gtactgaaca caagagatca agcagatcac
    65821 gcaagaagaa gcagcaagca aagtctgttt atcatcatga gaacaaacca ttctatcgaa
    65881 cgcaagcatg gaaagatatg cgtcaattta tttatgaaag agaaggtggt cattgtcagc
    65941 ggtgtggtca gttcatattt ggaaagagag cacacgtcca tcacattgta ccaatcaaag
    66001 acaacgaact gcttaagctt gatccaaaca atctcatgct attatgttca aaatgtcatc
    66061 caattgttga aaacgaaacg gaagacaaaa aagtttttcc ttcgtatttc aattgaagcc
    66121 cccctattca ttttcaaaat tttttcgcgt ggggagatag ggtagcgggg agtcacgcgc
    66181 atcgttaggt caaatttttc aaaaaacaaa ggggggtgta tacaaaaatg acgactaaag
    66241 cgcaacgtaa agcgattatt gatgaaaaag taagtgctga aaaagctcgt atcttagaaa
    66301 taatgaattt gtctgatttg tacaccatca ctcttgatcc attaatcgaa tcatacttgg
    66361 atatttttga aatttaccaa cacaaatatt tattgtggaa ggaaaaagga tttccggaga
    66421 cgcaaaaatt cacgaataaa tcaggagcta ctaatcaatc aaaacatcca ttggcacaac
    66481 aagtagaaac ttgggcagat aagaagatga aagctctaga tttattagga ttaacaaaca
    66541 aagcaaaaac tggtagacta attgctggag gatcgaccgc aagaaaagat gaagaaatta
    66601 cacgcccaga agtaaagcca gtagatgaac tagcagcgca cagaaataaa tggcgtaaga
    66661 aggcaggtgc tgaaaaatga ttgaacctgg tgtaaattat gctgatttat ttgcaaaaga
    66721 agtaagaaag aaacctaaga agtatcctaa gaccgttcgt ttagcaatag atcgctggta
    66781 tcagtggaaa aaaagaaaag atatttggtt tgatgtagat cgcgcgaatg aaatgatgga
    66841 ttgggtagaa tcatttattg ttcacacaaa aggagaaatg gtgggaaaac cgtttctttt
    66901 agaaccctgg gaaaaattta tttattcttg gatttatggt tgggttaaag aaaatgaaaa
    66961 aggccaaatt gtacgcgtta ctcgtgaggc ttatgtccag attccaaaaa agaatggtaa
    67021 aacattgatt gctgtcggtt cattaggata tgcaatgtac ggagaagggg cgttatcagt
    67081 tgattgctat gcttgcgcat ctgattttgc tcaagcgcaa tatgctgcca aaccttttgc
    67141 agttacaatc ttaaacaatc caatcctact tgaaggaact aaaatattta gggggccaaa
    67201 gggaactgtt tctagtatta cttatgacta tattcatgaa gatatggctt actcaaataa
    67261 atttattgtt caaaccaaga atattgataa tatcgaggga tccaatccat attttgtatt
    67321 gaacgatgaa ttgcataaac aggagaaaat ggaacagtat gacaatttta agtctgctca
    67381 gatatcttta ccacagccat tgatgtttaa tatctcaact gctgggaaag gttcgtcgtc
    67441 tgttggaatg cgtgtatatc gcgaagcaaa agaagtgttg aaacgtgatg ataatgattc
    67501 aaactttgtt ctaatctatg agccaaataa aaattatgat tggacggata gaaaagtctg
    67561 ggaaatgtgc aatcctaact ggggaatatc agtcgattta tctgctttag aatcggcatt
    67621 taaaacagcg caacggtcag ctcattccaa agctgaattt ctaacgaaac acttggatgt
    67681 atttgtgaac ggcgcggata atttctttga acaggatcaa gtagagccat gtttagttac
    67741 cacacaagaa cttggtgatt taagtggcga accatgttat atcggtttag atttatcacg
    67801 tacacgagat ttgacctgtg tatctttaaa cttcccaaca tgggatgaag atggtaaagc
    67861 ggttcttaaa gtaaagcaac tttattttat tccgaatgaa gatttagagt ttcgagaaaa
    67921 agaagataat gttccttaca gtgatttggc tgaacaaggt ttcgttgaat tttgcgacgg
    67981 aaaaatgatt gatcaagatc agattttgca gtacatagag gactgcatga atttatatga
    68041 tattcagcaa gtgaattatg atccagcgat gagtgacaaa cttgttgaga aattagaaaa
    68101 cttaggattg gaatgcgttg aagttgctca atatcctaaa gtattgaatg ctccttttga
    68161 cgatgtcgaa cggttgtttt atgagaaacg aattcaattt gataatccat tattccttta
    68221 ttgcactttg aacgttgtag caattactaa catcaatgga caaaaagcgc caagtaaacg
    68281 tcaatcaaag aaaaagattg atgggttcgt ggcgtttttg tgtggtcata aggaaacgat
    68341 gaatcaaatg acagatattg attcagatga gttagatgat tacctaagtt ccatctatag
    68401 ataaatagaa aggcggtgaa aaaaattgaa attacgtgat cggttatcga atgcagtcta
    68461 ttcttttatg gaaaaacgtg gatatatcga ggatattttt ggacactaca cacgttatgg
    68521 tcaaagatat gtgacggatt catctatcat ggaatcttct gatgtttatg aattagttca
    68581 agacatatct aatcaggtgg cattggccac accaattgtt attggtcccg acggcaacga
    68641 agtcaaagat catcacttac taaatatttt gaagagtccc aatgattatt tgacaggatt
    68701 cgaatttacc aaattggaaa caaatacttt attgatcaat ggtgagacat tcccattaac
    68761 ggatagggat cagcttcatt tggcgtacgg tgtaacaact aaaatcaatg aacgacttca
    68821 agaagaattt gaaatgaatg gtcaaaaaat acctggtcaa atgattcgac acattaagaa
    68881 catcggaact gattcattaa aaggtgctgg aataattgat cttgcaagaa acactctaga
    68941 aggcgttctg agcgctgaaa aagttttgac ggacaaatat actaaaggcg gtttacttgc
    69001 gttcatgctt aaactagacg cccacatcaa tccaaataat agcgcccaaa caaaaattgt
    69061 caaagctata ttggatcaac tggaaggaac gcaaaatgag agtgatcatt ctgttaagat
    69121 gattcctttg ggaaaaggat attccatcga gacattaaaa agtcctgttg atgacgcggc
    69181 aattttgaat tatttaggtg tttacaaaaa agacttagga aaatttctag gaatcaatgt
    69241 tgatacgtat caatcgctga tgaagacaga tattgaaaaa gcgatgatgt atctgcacaa
    69301 caaagcaatc aaaccaatat tgaaaaacaa gagcgaacat tacaccgctc ttttttttat
    69361 gcctaattct ggctatagag tggaatggaa aattaatatt ttggattttg ttccttactc
    69421 aacaaaaaca aatattggct acaacatcgt tcgtaccggg attacaagtc cagataacgt
    69481 ggcagaaatg cttggttttc ctaaacagaa tactccagaa acacaagcta tctatatttc
    69541 aaatgactta tctaggattg gccagaaaaa tgcaacagat gattccttac caacgaatga
    69601 tcaagacttg aaaggaggtg atggaaatga agaagaagga aattcgcacg attgacatca
    69661 ccaatctttc aacacgttcc gatgaagaaa ctcatacgag gaccattagc ggctacgctg
    69721 ctgtattcaa cagtcaaaca cttttatggg atgacttaag cgaagtgatt gttcctggtg
    69781 cattctcaaa ggcaattagt aattcggatg ttcgttgttt gttcaatcac gattggtcca
    69841 atgtgctagg acgtactaaa agcggaaccc ttcgccttga agaagatgaa cgtggtttga
    69901 aattcgaagt tgatttaccg gacacaacgg tagcaaggga cttagttaaa tctatggaac
    69961 gaggagacat caaccaatgt agttttggtt ttgtgccgac tgaagaaacg tgggactaca
    70021 attctgaacc aatgcttcga acaatccatg aagtggaatt atacgaggtt tctattgtgc
    70081 ctttgccagc atacgaagat acagaagctg cattaagaag tcgtgatgaa ttagaaaaaa
    70141 acgtcgaaga aagaaaaaaa ttaatcaaaa aaattaatca agcgctagaa gcgtaggagg
    70201 aaaaatacat ggataaagaa ttattgaaaa aaatgaaggc acgtcgtgag caacgattga
    70261 ctgaattacg tgaaaaagtt gaatcaggag aattacgcga agcagattta gaagccgtga
    70321 aagaagaaat tgacagtgtt attgatgaat tgaacggaat taaagacgaa ttaggcgcag
    70381 attctggaac tgatgaaaca gacgacaata cagatgatca atcgaatagt acggattcag
    70441 acgaaagccg ttctggtgaa gacaacgatc aagaagaaga ttcagacagt gaagatagtt
    70501 cagaaaatcg ttctggaatg attactcaac agcaacgaga tggattactt ggatcaatta
    70561 agaacggatt ggaggcacgt gcaaaaatga ccaagaaaca aaaagatcaa caactacgaa
    70621 aagcatttgc taattttgta gctggaaata tttctgaagc agaagctcga gctttaggga
    70681 ttgaagctgg caacggttca gttactgtcc cagaagtaat tgcatctgaa gttattactt
    70741 atgctcaaga agaaaattta cttcgtaaat acggaacagt ggtgcgaaca tcaggagatg
    70801 tcaaatatcc aattcttgtg aagaaagcag atgcgaatgt aaacaagaaa gagcgttcaa
    70861 ctgatattgc tgaaacagct attcagtttg atgaaatttt gcttaatcct gccgaattcg
    70921 atgctttggc aacagtaact aaaaaattat taaaaatgtc tggtgttcca gttgaagata
    70981 ttgttgtgga agaattgaaa aaagcttatg tgcgtaaaga aatcaattat atgttcaatg
    71041 gtgatgacgc tggaaatgaa aatcctggtg cattagccaa aaaggctgta gcatttaaaa
    71101 aacctttaga tctaactgct tcaggtgctg ggcaaaaatt atatgatgca ttaatcgaat
    71161 ttaaaaatac accagtgaca gaagtgatga aaaagggacg ctttattatt aatcgagctg
    71221 ctttgactgc cattgaaaaa atgaaaacag atgatggatt tcctttgttg cggccattca
    71281 cacaagcaga aggtggaatt ggttaccaat tagttggcta tcctgtggat tggacagatg
    71341 cagcagataa aaagggtgaa ccagacacgc cagttttata ttttggcgat ttttctgcat
    71401 ttaaaattca agaagttatt ggtgccttgg aaattcaaaa acttgttgaa aaattctctg
    71461 gtaaaaatca aattggattc caaatttaca acttgctaga tggtcaattg gtttattctc
    71521 catttgaacc ggcagtatat cgctacgaaa ttacaaaacc agttggtggt taagatggaa
    71581 gagcaaacta aagaattgtc tttagaggaa aaattcaaat cacatattca ttttgaagag
    71641 ggcatggatg attctttgct ctctttttat ttaaatatgg caaaagatta tgtcaaaaca
    71701 gcaactggtg gccaacaaga atatcttatt ttgatggttg ccggcattgc ctatgaatat
    71761 agagtttcag aggatgaact cgacaaagct atgaatgcca tgacgccatt tatcgtgcaa
    71821 ggagcgattc aaaatgccga agagacagac taataatctc agatggaaag ccgaattgct
    71881 agacatcaaa acaggaacag acggaaacga tcgtccaact acaatttacg aatttaagcg
    71941 tctaatattc tatgaagaac tcggtgtgac ttctcaagaa aaatatttat cacagcaagc
    72001 caagacagac gttgtcagac gaattaaagt cagatgggat aaatccatca cagagaaatt
    72061 aagtgcgctc aaaattgatt ctgtaacgta taacattact cgcatttata cgaatcccga
    72121 tgcaagagaa atggagttga gtttagctta tgtcgattag ctttgatgaa ttgaaaacag
    72181 cgctgaaatc aacaaagtta gcagtgttca gagacaaagc cagattaggg acgatgtatc
    72241 catacattgt gtactcaaat gtgagtaaca gcaaaaaaat ggcatccggt aaagtctata
    72301 aaaaattacc ttattaccaa gtctcttttt ttacacttgg aacagaacag gatttagttg
    72361 ttattgaaca agcgctgcaa aatgctggca ttccatattc agattttaca ggtatacaag
    72421 gtgatgagaa cgacgatacc gtaaccaact attacacata tgtgaggtgt gtggaaaatg
    72481 ccaagtaata agaatggttt ttctgaaata tcggattatt taggaaacct ttcaaaagtt
    72541 gatccgaaaa aattatcgtt ggaatcttta gaagaagcag ctaactttta tctaaagcag
    72601 ttgcttccta atattcctaa gtcgctctta aaaaagaaac atatgcgcga tcaaataaaa
    72661 gtagtagttg aaaacgatca agtgaaagtt caatttgaag agacggcctt ttactggcgc
    72721 tttgccgaaa atggcacaac aaatcaacgt gcccaacatt ttgcaagtgg tacgtatgaa
    72781 caaaacaaag aaaaaattga agaaattatg actagaaaaa tacttgattt atgggaaggg
    72841 tgattttaat tgggaaaaca agatactttt tattttgaag gattggacga cattttaatt
    72901 gcgatgatgg caacgccaga ttctgttgga actgcaccga cttatagtga agttttacga
    72961 ttgccaattg ccacaaaaat cggtgtcaaa gggaatggaa cagcattaga aaaatgggca
    73021 tccagcaaaa tgtttcgtcg tgtgtcgcga gaaacaaaac atgaacttgc gttggatcac
    73081 gtgggtattc cgatagaagt gatggatgaa atcaaaggat tagttgctaa aagcggagta
    73141 acgtttagca aaaataccgc acgagaattt ccgtacttcg cctttggatt tattggaaat
    73201 attgaaaatg gcggtaaaaa agcagtttgg tatcctaata cacaactatc caatgtgatt
    73261 gacgaagagt atgccactgc agaggaagaa acaaaaattg acgatgtaac tgcaaatctg
    73321 gtttccatcg gactgaaata caataatgtg atgtatgcaa gttttgattc aaatagagac
    73381 ggagcttctc tagaattatt taataaattc attgctcaac ctgtatacga tgaagaacag
    73441 tggaaaacat tggctaaagt tggaggtgcg ggttaatggc tcggttatct gattatggaa
    73501 ttcacgttga agacttaaaa aattctgctg ttgtaattat tcaaggcgca gaattcccta
    73561 tttcatttac tatgcaaacg atggaattta tagcagatgt gtatggtgga gattattcgc
    73621 aatttgaatc tgatatgaac gccatgctat ctaaaaaaga aggacaaatt tcttctgcta
    73681 acttatcgcc tagtgatcta aaaatcatgc gtgccttgat ttatgccatg ctgcgtactg
    73741 gtggtttaga ggaagatcca gaaactattt tcaaattttt gggaatgagt ggagaggtgt
    73801 tgtctgctta tagtacctgt atggaaattt ttgctagcca gacatttcag gtggaagacc
    73861 taaaaaaatc caagaagcca caagactttc aaaaagcgca agcaaaaaga aagaaaaaca
    73921 aaaagaatcg gaagagatag gaactccttg gagtttttat atttacgttg ctctcactct
    73981 attgaattgg agtgagagtt tctttttaaa gtctacacct aacttgtggc tcaaatctta
    74041 tttacagtgg ttacaacaaa acaccgattt tgaaccacct caatctgtaa ctatggataa
    74101 atcgccttgg tggtagaaag gagcgctaag ataaatggct ggtaaagaat ctgatgtcgt
    74161 tcttaatttt aaaacgaatg gcgaagtcag ttattcgaaa acaatcaaag aaatcaacaa
    74221 agaaatgaac ttagccgctg ccgagtacaa aaaccaagtg tctgcgatgg acaaggatgc
    74281 aactcaaaca gaaaaattgc gagcggctaa acaaaaatta gaaaaacaat taggattggc
    74341 tgaacaacga tctcaaatgc tgagagagga atatgaaaaa tctgtcaaag aaaccggtga
    74401 atattctgct gaatcagaaa aactttataa acaattactc aattctgaaa ccggagaaaa
    74461 taaacttcgt acagcattag aacagacgaa cgatgcactt aaagaacaag gtgacgtttc
    74521 tgttgataca gcaaaaaaac tccagaaaat cgaagaaaca ggtgagaaag taaaaggcgt
    74581 tggtgaaaaa atgtctgttg gagtaaccgc gcctattgta gcggcaggag cagcaggact
    74641 tgcagcattt ggtgaagttg acgaggcact tgataccatc attacaaaaa ccggagcaac
    74701 aggtgatcaa gctgatagac tttcacagtc tttcaaaaac gttggttcaa atactcattt
    74761 acctttacaa acggttgggg aagctattgg tgaggtaaat acacaatttg gattcatgga
    74821 taaaaaactg gaagattcaa ccaattatct cctacagtac gctgaaatca atgatacaga
    74881 tgtttcgcaa tcagcaatat ctgctcgaca agctattgat gcttatggac tagaatatga
    74941 tgatttgaat tctgtccttg atgtaacaac gaaaacatcg cagaatactg gtcaatctgt
    75001 agacgacttg atgcaaaaag caattgatgg cgcaccacaa attaaacaat tagggttgag
    75061 ctttggtgaa ggggtcactt tacttggaca atttgagcaa tctggtgttg attcaagcgc
    75121 agctttaagt agtttatcta aagcaacagt ggcttatgcg aaagacggaa aatctttaag
    75181 tcaaggacta gaagaactac aagacaaggt aaaaaatgca ggttctgaaa cagaagccat
    75241 caatgcagca gcagaagtat tcgggactaa aggcggtcct agaatggccg atgcaattcg
    75301 tagaggtact ttgaacctgg aagatttagc aaaaactgct ggagaaagtg ggggagctgt
    75361 aggagacaca ttcgatgcta ctctcgaccc aattgatcaa gccgatcaag ctatgaacaa
    75421 tgcaaaatta gcaatggctg acgtaggtga agcagtccaa gtaagccttt tgcctttttt
    75481 tgaaaaagca acttccttat tacaggaatt ctctaaatgg tgggggtctc tagatcaaga
    75541 tacaaagaat tggatcataa caattgctgg tatagcggca gcagttggac cagtactaat
    75601 cgttcttggt tcacttatgg gatctgttac aaaaatcgtt ggggaagtta aaagttttat
    75661 aggagtttgg caaggacttg ctggtttatt tggaatgtcg ggaggctggt ttgcattagc
    75721 agttatagct attggcgcat tagttactgg actaatttgg gcatataata acgttaaatg
    75781 gtttcatgat ggagtgaatt ctttcttcaa aggtgtttca gatgtagctg ttgaaatatt
    75841 taatttttta agtggttatt ttggtggatt tttcgatggt gttatagcaa atttcaataa
    75901 tttttataat gctggaaaac gaattttcag tggttttcta gattttatca ctggagtatt
    75961 tacagggaat tggtcaaaag catggcaagg tctagttgat atttttggag gtattttcga
    76021 tggtattgtt gctgtaggga aagctcctat taatgccatg attggattga tcaatggatt
    76081 tattggtgga ttgaataata taaagatacc aaaatgggtt cctggtgtag gtggaaaatc
    76141 attttctatt tcaaaactgc cttatttagc acaaggcggt catctaatca atggacaagc
    76201 gattgttggt gaagctggac ctgagttact gactgccaag aatggtaaaa ccacagttac
    76261 tccattatca gacgaagaaa aacgcaaagg gatcagtgga agagtttctg gaggtagcat
    76321 tgaacagcat atacacattg gcaaagtcga tgcaaacaat ccatctgagt tagatcgtat
    76381 gaatcgtaaa tttgctaaag caaacaaaca ggctattcgt gatttgggag gtgttcctat
    76441 ttgagtcgac aatttatgaa tccagatgaa ccaaatttta tttggaaaaa tcggaatgca
    76501 gtaattgata tgaattgcat cattgaggca gagcttccag atattatgcc gaacaagcga
    76561 tacgaaactt acacgattca gggccgaagc ggagaattga cggagacgtt tgatgactat
    76621 gaaccttttg atttggaaat agaagggatc actattccac attcgaaact gagagaagtc
    76681 aaacgatggc tcactggtaa aagccaatta atcacacata atgatccaga caagtatcta
    76741 gaggctatct gcagcatgga taaagaagtc cctttcgaaa acgagtgggg cttcttttat
    76801 acattcgatg ttacttttcg ttgtcagcca ctcaaaagaa aaatcggtga agcacctaaa
    76861 atttttcatt ctagtgttat ggatattttt gatcatggtg acgaaattgc acatccctat
    76921 ctggaaatag aatcgaacgg aggagatata acgatcgctg tcggtgataa aagcctaaca
    76981 atacttaata cgttagctgc aacaatcgtg gttgatactc agcttggaaa agcaatacaa
    77041 gaaggtttga atttatttac gcgtggtgat tggccagtat tgcagcctga atggaatcag
    77101 gtaaaaatat ccggaaaatt taaagaagtg cggttttgga ataggagtgt gtatctgtga
    77161 cgcaagaatt tatttatgct tataaaaaaa tgccagatga tttgagcgtg aacggagcgt
    77221 cgttagttga ttgggaagat ttgccagaaa tcaatcgtgt gctgaatggc caatatcgtt
    77281 tttatggtaa ctattcaaga agcggacagt atcgctctta tttaaaaaaa ggaaatttta
    77341 taaaagcgaa ggtaccagat ggatcatggc aatattttga aatttataat gtcaaaaaga
    77401 atttgacctc cgtttctgta acagctcggc atattggttt tatggccaat aagaacttta
    77461 ttgtcaagtc gtttacggat aacggcaatg ggtctcagat catgaccaat cttaaaaaca
    77521 gtttggcatt tgatcaaaaa tttaactatc tttcgaatgt agggacaacg caccaattta
    77581 cagcaagaca agtcgcgcca gttgaagcaa tcattggttc aaataacggt aatcaaaatt
    77641 tgactggcgt gactagcgcg gaacttgata tggataacta tgatttgaaa ctggtaaaac
    77701 agattggatc agataatggt tttcggattg attttggcat caatttagaa gcaatcgaag
    77761 aagaaattga tgaagaatca attgtcaata gtttgtattt ggttggcggt gttcctgata
    77821 acgattatga tgaagacaaa gaacctatcg aatatggtta tttagaaatt gatggagtaa
    77881 ccaacgagaa tcgacgtatt gccaaacgtg aaaacggaga ttgtaaaaca gttgatgaat
    77941 tgatcaaatg gggcaaaacc ttatttgaca atgatcgtat ccatgagcca aaagctactc
    78001 atacagtcag tatggtggcg ttggaacaca cactcgaata cggtgacatg tatcaagagc
    78061 tagcgtcttt acactttggt gatgtcgctc atgttagggc gaaagagcta gatatcgaga
    78121 taaaagagcg tatggtcgaa tacacttatt tcccaacgct tgggaaatat aaggatattg
    78181 ttttaggtaa tgatttatca ctgtacactt cggcagtaaa tactcaagca caagaattga
    78241 aaaagaaaat cgacaatcga acagaaacgt tagttcaaaa tgtgttgaat gcgacggcat
    78301 ggatcactgg taattctggt gggcatgtcg tttttcgtcc agaaaaagct ccatcagaga
    78361 ttcttatcat ggatactcca aatgtggcga atgcaaagcg agtgtggcgt tggaatttaa
    78421 atggcttagg ttattcagac aacggagtaa atggcccgtt cggtattgct atcacatcaa
    78481 aaggagaaat tgttgctgac ttcatcaaag taggaacaat aaatgcagaa gtattcgaaa
    78541 cttcctttaa tgcttatggt gatgtgttaa aacttgtaaa aggcacgctt caaatttgga
    78601 atgaaaacaa aaaaatcatg gagctaacca aaaaaggtat ggaattctgg aatagaaaag
    78661 aatccattgg aacgatcgga acaacagatt cagcaggaaa tccatttcca aatgctgtaa
    78721 cacctacacc tcttgaggaa aattctttgg taatacgtac aaatggcgat ggaaaataca
    78781 ttttaatttc tccaactgca gaaaaaggat ttgtgttatt aggaaacgga aaagcatatt
    78841 atttcggtga tttagatatt cagggaaaac ttactgttag aggtaaagaa gtgataccag
    78901 gacaaaatgg cggtccatct ggcggaggag aaacacctgg tgggtatcct gatgaattaa
    78961 aaacagatgc agaaaagaga gcttggagaa tatacgatat tttgtgtaat aacggattta
    79021 cgaaacaatc tgcatgtgga atattgggga acattcaaca agaaacagga ggaacttttg
    79081 atcctgatac tgttcaaata ggtggaccag catacggatt agttcagtgg gatggttctt
    79141 catatcctct tgttggtcca gcaacatggg atggaaaagt ttatgttcaa aacttattta
    79201 atgctgcagg tattaaagaa ccaataacga gcttagatgc acaagttcga ttgcttattt
    79261 ggacatttac aaatggacaa tggatgg
//
LOCUS       DACCYH010000003        74341 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.003, whole genome shotgun sequence.
ACCESSION   DACCYH010000003 DACCYH010000000
VERSION     DACCYH010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 74341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.003"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(7..1581)
                     /locus_tag="IVY85_000170"
     CDS             complement(7..1581)
                     /locus_tag="IVY85_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043215.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(1836..2345)
                     /gene="mreD"
                     /locus_tag="IVY85_000171"
     CDS             complement(1836..2345)
                     /gene="mreD"
                     /locus_tag="IVY85_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP8043216.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(2349..3203)
                     /gene="mreC"
                     /locus_tag="IVY85_000172"
     CDS             complement(2349..3203)
                     /gene="mreC"
                     /locus_tag="IVY85_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP8043217.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(3358..3996)
                     /locus_tag="IVY85_000173"
     CDS             complement(3358..3996)
                     /locus_tag="IVY85_000173"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP8043218.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(3997..4647)
                     /locus_tag="IVY85_000174"
     CDS             complement(3997..4647)
                     /locus_tag="IVY85_000174"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP8043219.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(4660..5559)
                     /gene="rsgA"
                     /locus_tag="IVY85_000175"
     CDS             complement(4660..5559)
                     /gene="rsgA"
                     /locus_tag="IVY85_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP8043220.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(5724..7793)
                     /gene="pknB"
                     /locus_tag="IVY85_000176"
     CDS             complement(5724..7793)
                     /gene="pknB"
                     /locus_tag="IVY85_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP8043221.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(7790..8530)
                     /locus_tag="IVY85_000177"
     CDS             complement(7790..8530)
                     /locus_tag="IVY85_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP8043222.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(8543..9901)
                     /gene="rsmB"
                     /locus_tag="IVY85_000178"
     CDS             complement(8543..9901)
                     /gene="rsmB"
                     /locus_tag="IVY85_000178"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP8043223.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(9901..10839)
                     /locus_tag="IVY85_000179"
     CDS             complement(9901..10839)
                     /locus_tag="IVY85_000179"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP8043224.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(10832..11323)
                     /gene="def"
                     /locus_tag="IVY85_000180"
     CDS             complement(10832..11323)
                     /gene="def"
                     /locus_tag="IVY85_000180"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8043225.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(11338..13746)
                     /gene="priA"
                     /locus_tag="IVY85_000181"
     CDS             complement(11338..13746)
                     /gene="priA"
                     /locus_tag="IVY85_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP8043226.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(13999..14304)
                     /locus_tag="IVY85_000182"
     CDS             complement(13999..14304)
                     /locus_tag="IVY85_000182"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8043227.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(14309..14923)
                     /gene="gmk"
                     /locus_tag="IVY85_000183"
     CDS             complement(14309..14923)
                     /gene="gmk"
                     /locus_tag="IVY85_000183"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP8043228.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(15410..15694)
                     /locus_tag="IVY85_000184"
     CDS             complement(15410..15694)
                     /locus_tag="IVY85_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043229.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(15712..16602)
                     /locus_tag="IVY85_000185"
     CDS             complement(15712..16602)
                     /locus_tag="IVY85_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP8043230.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(16622..16840)
                     /locus_tag="IVY85_000186"
     CDS             complement(16622..16840)
                     /locus_tag="IVY85_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043231.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(16810..18510)
                     /locus_tag="IVY85_000187"
     CDS             complement(16810..18510)
                     /locus_tag="IVY85_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP8043232.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            18777..19550
                     /locus_tag="IVY85_000188"
     CDS             18777..19550
                     /locus_tag="IVY85_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043233.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(19631..20200)
                     /gene="thiT"
                     /locus_tag="IVY85_000189"
     CDS             complement(19631..20200)
                     /gene="thiT"
                     /locus_tag="IVY85_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP8043234.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(20266..20368)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(20374..21345)
                     /locus_tag="IVY85_000190"
     CDS             complement(20374..21345)
                     /locus_tag="IVY85_000190"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8043235.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(21439..22812)
                     /gene="glmU"
                     /locus_tag="IVY85_000191"
     CDS             complement(21439..22812)
                     /gene="glmU"
                     /locus_tag="IVY85_000191"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP8043236.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(23315..24133)
                     /gene="purR"
                     /locus_tag="IVY85_000192"
     CDS             complement(23315..24133)
                     /gene="purR"
                     /locus_tag="IVY85_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP8043237.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(24300..25124)
                     /locus_tag="IVY85_000193"
     CDS             complement(24300..25124)
                     /locus_tag="IVY85_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8043238.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(25097..25777)
                     /locus_tag="IVY85_000194"
     CDS             complement(25097..25777)
                     /locus_tag="IVY85_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8043239.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(25800..26738)
                     /locus_tag="IVY85_000195"
     CDS             complement(25800..26738)
                     /locus_tag="IVY85_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP8043240.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(26983..27834)
                     /locus_tag="IVY85_000196"
     CDS             complement(26983..27834)
                     /locus_tag="IVY85_000196"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP8043241.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(27988..30561)
                     /gene="mprF"
                     /locus_tag="IVY85_000197"
     CDS             complement(27988..30561)
                     /gene="mprF"
                     /locus_tag="IVY85_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8043242.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            30702..31508
                     /gene="proC"
                     /locus_tag="IVY85_000198"
     CDS             30702..31508
                     /gene="proC"
                     /locus_tag="IVY85_000198"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP8043243.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(31587..33143)
                     /gene="rny"
                     /locus_tag="IVY85_000199"
     CDS             complement(31587..33143)
                     /gene="rny"
                     /locus_tag="IVY85_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP8043244.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(33554..34603)
                     /gene="recA"
                     /locus_tag="IVY85_000200"
     CDS             complement(33554..34603)
                     /gene="recA"
                     /locus_tag="IVY85_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP8043245.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(34712..35953)
                     /locus_tag="IVY85_000201"
     CDS             complement(34712..35953)
                     /locus_tag="IVY85_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP8043246.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(36193..36771)
                     /gene="pgsA"
                     /locus_tag="IVY85_000202"
     CDS             complement(36193..36771)
                     /gene="pgsA"
                     /locus_tag="IVY85_000202"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP8043247.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(36980..37864)
                     /locus_tag="IVY85_000203"
     CDS             complement(36980..37864)
                     /locus_tag="IVY85_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8043248.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(38642..39928)
                     /locus_tag="IVY85_000204"
     CDS             complement(38642..39928)
                     /locus_tag="IVY85_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8043249.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(39921..41189)
                     /locus_tag="IVY85_000205"
     CDS             complement(39921..41189)
                     /locus_tag="IVY85_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8043250.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            41441..41902
                     /gene="mscL"
                     /locus_tag="IVY85_000206"
     CDS             41441..41902
                     /gene="mscL"
                     /locus_tag="IVY85_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP8043251.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(41961..42407)
                     /locus_tag="IVY85_000207"
     CDS             complement(41961..42407)
                     /locus_tag="IVY85_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8043252.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(42500..42745)
                     /locus_tag="IVY85_000208"
     CDS             complement(42500..42745)
                     /locus_tag="IVY85_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043253.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(42860..43438)
                     /locus_tag="IVY85_000209"
     CDS             complement(42860..43438)
                     /locus_tag="IVY85_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8043254.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(43515..44045)
                     /locus_tag="IVY85_000210"
     CDS             complement(43515..44045)
                     /locus_tag="IVY85_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP8043255.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(44049..44909)
                     /gene="rlmB"
                     /locus_tag="IVY85_000211"
     CDS             complement(44049..44909)
                     /gene="rlmB"
                     /locus_tag="IVY85_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP8043256.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(44902..45297)
                     /locus_tag="IVY85_000212"
     CDS             complement(44902..45297)
                     /locus_tag="IVY85_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8043257.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(45294..46706)
                     /locus_tag="IVY85_000213"
     CDS             complement(45294..46706)
                     /locus_tag="IVY85_000213"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP8043258.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(46703..47239)
                     /gene="cysE"
                     /locus_tag="IVY85_000214"
     CDS             complement(46703..47239)
                     /gene="cysE"
                     /locus_tag="IVY85_000214"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP8043259.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(47561..49021)
                     /locus_tag="IVY85_000215"
     CDS             complement(47561..49021)
                     /locus_tag="IVY85_000215"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP8043260.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(49191..50348)
                     /locus_tag="IVY85_000216"
     CDS             complement(49191..50348)
                     /locus_tag="IVY85_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP8043261.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(50382..51752)
                     /gene="radA"
                     /locus_tag="IVY85_000217"
     CDS             complement(50382..51752)
                     /gene="radA"
                     /locus_tag="IVY85_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP8043262.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(51815..52342)
                     /locus_tag="IVY85_000218"
     CDS             complement(51815..52342)
                     /locus_tag="IVY85_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP8043263.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            52572..53378
                     /gene="proB"
                     /locus_tag="IVY85_000219"
     CDS             52572..53378
                     /gene="proB"
                     /locus_tag="IVY85_000219"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP8043264.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            53375..54622
                     /locus_tag="IVY85_000220"
     CDS             53375..54622
                     /locus_tag="IVY85_000220"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP8043265.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            54698..55042
                     /locus_tag="IVY85_000221"
     CDS             54698..55042
                     /locus_tag="IVY85_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043266.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(55159..55485)
                     /locus_tag="IVY85_000222"
     CDS             complement(55159..55485)
                     /locus_tag="IVY85_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043267.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(56100..57302)
                     /locus_tag="IVY85_000223"
     CDS             complement(56100..57302)
                     /locus_tag="IVY85_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8043268.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            57419..57934
                     /locus_tag="IVY85_000224"
     CDS             57419..57934
                     /locus_tag="IVY85_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8043269.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(58133..58579)
                     /locus_tag="IVY85_000225"
     CDS             complement(58133..58579)
                     /locus_tag="IVY85_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8043270.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(58626..60104)
                     /gene="msr"
                     /locus_tag="IVY85_000226"
     CDS             complement(58626..60104)
                     /gene="msr"
                     /locus_tag="IVY85_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8043271.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(60458..61087)
                     /locus_tag="IVY85_000227"
     CDS             complement(60458..61087)
                     /locus_tag="IVY85_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8043272.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(61071..61535)
                     /locus_tag="IVY85_000228"
     CDS             complement(61071..61535)
                     /locus_tag="IVY85_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8043273.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(62022..63290)
                     /locus_tag="IVY85_000229"
     CDS             complement(62022..63290)
                     /locus_tag="IVY85_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP8043274.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(63521..64258)
                     /locus_tag="IVY85_000230"
     CDS             complement(63521..64258)
                     /locus_tag="IVY85_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8043275.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(64325..64894)
                     /locus_tag="IVY85_000231"
     CDS             complement(64325..64894)
                     /locus_tag="IVY85_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP8043276.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            65022..65894
                     /locus_tag="IVY85_000232"
     CDS             65022..65894
                     /locus_tag="IVY85_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8043277.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            66236..67108
                     /locus_tag="IVY85_000233"
     CDS             66236..67108
                     /locus_tag="IVY85_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8043278.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            67105..69063
                     /locus_tag="IVY85_000234"
     CDS             67105..69063
                     /locus_tag="IVY85_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8043279.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(69395..70246)
                     /locus_tag="IVY85_000235"
     CDS             complement(69395..70246)
                     /locus_tag="IVY85_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8043280.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            70369..70836
                     /locus_tag="IVY85_000236"
     CDS             70369..70836
                     /locus_tag="IVY85_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP8043281.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(71099..71581)
                     /locus_tag="IVY85_000237"
     CDS             complement(71099..71581)
                     /locus_tag="IVY85_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8043282.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(71681..72544)
                     /locus_tag="IVY85_000238"
     CDS             complement(71681..72544)
                     /locus_tag="IVY85_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8043283.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            73142..74017
                     /locus_tag="IVY85_000239"
     CDS             73142..74017
                     /locus_tag="IVY85_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043284.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(74162..74314)
                     /locus_tag="IVY85_000240"
                     /pseudo
     CDS             complement(74162..74314)
                     /locus_tag="IVY85_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
BASE COUNT        20906 a        15889 c        13324 g        24222 t
ORIGIN      
        1 tcattcttac atgctgacag caaagtcagg ggcaaaccat tgaactgaac caacttttac
       61 gctttcgcct ggttgaggag catggatata ttgtcctccg cctaaagcaa tcgctacgtg
      121 gtaagttccg ccttgtgacc cccagaataa caagtctcca gcttttgctt gagaaactga
      181 aatttttgtt ccagctgatt cttgaggaac tgtccaacca ccgatgtcac gaccagttac
      241 ttgcatgtaa acatagcgtg tgaaccctga gcagtcaaag ccacttggat ctttaccgcc
      301 ccaaacatat ggagtaccaa tatatttgta agcttctgct acgattgctg caccattcac
      361 acttccagaa ggagctggag caggtgttac aggttttggt tgttcaggtg ttggtgttgg
      421 cgttggagcc ggagtactac ttcctgtgcc gtttcctgta tcaacacttt gatctgttga
      481 tggtgttgta ggcgcaggtg ttgttgattc ttctgttgtt gaagattcag gcactgttgt
      541 tgattcttct gttgctgaag attcaggcac tgttgttgat tcttctgttg ctgaagattc
      601 aggcgctgtt gttgattctt ctgttgctga agattcaggc gctgttgatt cttctgttga
      661 agattcaggt gttgttgatt cttctgttgc tgaagattgt gtagctgatg atgattcttc
      721 agtcattgaa gattgtgtag ctgatgattc ttctgttgct gagctttgtg cagtagatga
      781 agcttgagta gcttgtgctt cagcctctgc ttgttcgcgt gcttcttttt cagctttttc
      841 ttgtgcagct tgttgacgag cttgttctgc tagacgttgt tgttcacgaa tacgagcttg
      901 ctctgcttca gcttctgctt tttgacggtt caagtcagct ttcttgtctt cagcagttgc
      961 ttgttcagca gctaatgaag ttttcagtac gttcaaatca gcttgtttag ataaaagatc
     1021 gcctttttga gattccaaag cagcttggtt ttcagctaat tcttttagtt tagcatcgtt
     1081 ttcagctttt ttgtcttcta cagctttttt atcttgtttt tgttgttcca tcaagtcgtt
     1141 gtttgctttt accatcgttg tcattgcttg aacgcgtcca atagcatctg ctaatgaatc
     1201 agcattcaat acagcatcga tgtagtttga gcttgtgttg cttacttgtg cttcgcgtgc
     1261 ttgtttttgg attgtatctt cacgtttttc aatacgttct tgtaaatctg caatgtcttt
     1321 tactaattgt gcagattctt gacgcaatgt atcttgtttt gctaataggt cttgtgcttg
     1381 tgtgttgatt tcagaaactt ggctttctaa ggcatcgatt tgtgattgtg catctgcttg
     1441 ttgatttttc aagtctgcaa tcttttgatc ttgctgttgt atctgagaat caaaatcgtc
     1501 cgccgcggca gcgatcggtg atgctacggc agtcaaggtc attgaacata ccattactgc
     1561 tgatattaaa ctctttttca ctcttcattc ctccgactgg cttaatttaa taaatatcta
     1621 aatatattta agtctttctt aacgatataa aaattgtacc atagcagtgt gtcaacgata
     1681 taatagttat gttacaaaaa tgtttcaaaa caacttttaa aaatgacaaa actgttcgtt
     1741 tcagaaaatg atcatgaaaa agccgcgtta agtctagcat ttcttaagat aacaacgatt
     1801 ctttttcgct ttcgacctaa aaataaaaaa agaaattatt catcactgaa taatttctta
     1861 aagggaaaaa tgaataatac aaatataatc atgttcaaca gcagtgtagg tcctaaaaac
     1921 cttgtaataa aatacgtgtt atttactact gctaatttaa aaatcaactg gaccaccatc
     1981 gtaaacaatt cataacccgt gacaaagatg atcatactga aaaattcagt aaagatgttg
     2041 acatggataa cgtctttcat tacatacatc aaccatacaa ttaaagggag agctactgca
     2101 tagataccga taacgccaat atagtaagcg tcatagatag ccccgagaac aatcgtggta
     2161 atcagtaaat aacgtttttc aaacgccata ctgcaacata ataatgccaa tatcagaaag
     2221 tgggcatttg acatataggt gcctttcgac cactccccta gcattcttgt taaatgacca
     2281 tctatcaaca tcaaaaagaa caaaacgatt ggcagatagt acttcattgt ttctttcttc
     2341 aacatggact aatcctccgc tagccgttgg acaatcgtca caactgacac gtcatacatt
     2401 tctgcataag gtttcacata aacttcccga tccagcccat aactatctgg tttcgtttta
     2461 ataaccgtac cgatcggcaa gttagctgga gaattccctc caagaccgga tgtctggaca
     2521 acatcccctt ctttgatatc cgtatcacca gttaattggg tcaccactaa ggcatggagc
     2581 ttttcatcat agtttttaag caaaccaaac gcttcgccat tagccgaaga tacccgtact
     2641 ggaaaatgat tggagctttc attagaggat gacagtaatt cgattttaga cgaagccgta
     2701 ttgacctcga tcactcggcc aatcaatcct ttttgcgaca tgactgccat atttgcttcg
     2761 ataccgtctt tagaaccctt gtctacgatc agcatgtcct gccaactgtc aggcgaacga
     2821 gtaatgacat ttgccgttac tttttcgtaa ctagtcagcg tctgattcaa ctcaagttct
     2881 tttttcaacg cttcgatttc tctctcgtaa ttcttgtttt gctgtgaaac gtcatcatag
     2941 ctatcgattt tttcttttag ccgctcattt tcggcataag ttgaaaaaag attatggaca
     3001 gaatctactc catttcgtac ccaccgtact ggggcagaga ttgttttatc aaccattgca
     3061 acactgtcat taacgatgga ttggaagaaa ttgggctttc cttcattcgc tcgctgcgca
     3121 gcggtcacac ttagtatcgt caccacgatg ataacaataa ccaaggtaat aataatattt
     3181 ttattaggat tgaacttctt cacaaagaca cctcgaatta attagactct tgtgttcaat
     3241 tttatcacta aatgccgtga aacaaaaggt aagttgctcc tcttaagctt ttttttagca
     3301 aataatagat tctgtcattt ttagcctgaa agacaaattt ttttatttgt ttatggatca
     3361 atctttgctt tgaataactg caatcatacc atctgaaaaa gaaaattctg cggtatctga
     3421 aagaaattcg ttacttgcat aagatgtcgg atattctact tctttatttt ccaatacata
     3481 cttgctgcct tttaatgtaa gattcgagac gggagtcaag cagcaataag ctaaatactt
     3541 catatctggt atctgtctta ctatatgctc tccaggcaaa taataagaca aataattttg
     3601 acaatctctg ataataagct gatgggcaaa gggtttgaag cgaggttcca tacctaacca
     3661 gagattggct aaaagatgat ccaatcttcc gcccgtagct ccaatcaaag tgacttccgc
     3721 ttgcgggtaa cgttcaaatg tttttaagat acctaactgt gtatctgtat cgtctttttc
     3781 tgcaggggaa cgggtatact ctttggcttt tgcaaatact tccttctgct cttcgttcga
     3841 caatgaatca aaatcaccca ctgccagatc tactgggtag cctgcttgaa gcaggtacag
     3901 cgcaccacgg tctatcccga cataggcatc gtatttggac agcagagact gcggccagtt
     3961 ttgcggatta cctccagcag ctaataaaat tttcatttag tctaatgcct cttttaatcg
     4021 ttcgatttgt ccgactggat tttcggctcc atagatatat gatcctgcaa cgaagacatc
     4081 cgcccctgca tctttacaga tttttgctgt ttctggaacg attccgccat ctacttcgat
     4141 tgcataagtc caattgttct ctctacgtag atcagcgagt tccgtaattt tttctactgt
     4201 ttcttcaata aagctttgtc cgccgaaccc aggattgaca gtcatgacta gtacttgatc
     4261 agccaaaggc aaaacatgtt tgatcatgga gactggtgtt cctggattga taactactcc
     4321 agctttaact cctaagcttt ttgtcatttg aagtgcccga tgaatatgag gagttgcttc
     4381 gacgtggaca gtaatgatat ctgctccagc ttttgcaaat gctggaatgt agttttctgg
     4441 atcaacgatc atcagatgga cgtctaacgg taatttagta acaggacgga ttgcctgaac
     4501 aatatttggc cctaatgtga tatttgggac aaatcggcca tccattacat ctacgtggat
     4561 gtaatctgcg cctccttttt ccactaactc aatgtctctt tgtaaattgg caaaatctgc
     4621 actcaaaatg gatggtgcta tcttcattct tgtttcctcc tattttcgtt taccttcttt
     4681 ttttgcatac attggttttc ttttttcgat ttcttgtaag aattgcaaat aattgtcgta
     4741 acgtgtctgt gcgataccac ctgtttctac tctatgtttt acttcgcagc caggttcttt
     4801 agcatgcatg cactctctaa atttgcaatg gacagaagct tctacaaatt caggaaattg
     4861 tctcggcagt tctcctgctt caattgtcaa aaagtcaatc gagctaaatc ctggtgtatc
     4921 tgctactaac ccgccataga taggaatcag ttccacatga cgtgttgtat gccgtcctct
     4981 tccaagtgca tcagaaatct ctcctgttgc taaattcagt tccggtgata tttgattcag
     5041 tagcgtcgat tttcctgctc cagactgacc cataaagacc gatagccgtt ctgggaagta
     5101 acggatcagt tctgtcgtat cacccgtatg attgggtaca atcaccggat agcctatttt
     5161 agtataggtt tgcttgatct cttcgatttt ttctgggtcg tctgacaagt cggtctttgt
     5221 caaaaaaata atcggttcga tcgactcgta ttccaaggta actagaaagc ggtcaagtag
     5281 attataagaa aatgcaggct ctacgatact tgtgacaatg attccaagat caacatttgt
     5341 gactggtggg cgaactaatt gattttttct cggcaagatt tccagaagat agccgtctgt
     5401 ttgattttca ctctcaaaga tcacctgatc tccgacaagc ggcgtgattt tccgattccg
     5461 gaaatttcct cttcctcttg tttgataggt ctctccgttt gcgtatacat agtaaaaacc
     5521 gctgattgct tttctgattt gtcctttgat aagtgccacc tccgtttttg tttattttac
     5581 catacgaaga ggaaagtagg tgttattcca tgcaaacttc tcaaagtata tggcttgaaa
     5641 attgatcaaa agagatagaa acacaaaaaa gagaatgcaa caaatcaatg tcgcattccc
     5701 ttctgatata ttttattcag cacttatttt gaactttctg aggtgctagc tgatgaactt
     5761 gtggagctgg aagattcttc ttttgaagag ttgcttgtgc tactggagct acttgagctg
     5821 ctagaactgt ctgaagagtc tttttcagaa ctagaagacg ctggatctgg tcctttagag
     5881 taaattacgt tcacaaccat tcctttggta attgtcgttc ctgcccctgg tactgtacga
     5941 atcactttat ctttatccac cgtgtttgaa tattcggaag tctcatgccc gatatattct
     6001 gcaccgacac tagctaaata actttgtgct tcgctttttg tatagccgga gatatcattc
     6061 aatttaggct ctgttccttg gcttacgaca aaattgatgt aagtttcttc tgctacaact
     6121 tcacccgagg ctggatcctg actgatgatc gaacctgatg gtacagtgtc gctatagtca
     6181 taagtagcac taatattatt gatattgaat ccttgtgtaa caagccaatt acgaacagtt
     6241 tcgtagtttt ctcctacatg actactcaaa gtcactgttt ctggtccttt gctgactgtg
     6301 aaagtaatcg tgtctgtttt aggatcgact tctgtatctt ctgatggaga ttgcgaaatg
     6361 atcattccgc tttctacctc tgaactgaat tcctcttctt tttgaatcaa gtcagcagaa
     6421 aaccctaatt ctttcaaact tgcttcggca tcttcataac ttttaccact gtaatcatct
     6481 aatttgattt ttttcgtgcc gctactaata tacagagtga cttcgcgttt ttgcttgact
     6541 gtcgttcccg ctgccgggtc tgtcttcact acattacctt cttcgatttt gtcatctgca
     6601 atttcttctg tttttgttgc tacttctaat ttcgcttttg cgagtgtttc tctagcagaa
     6661 gcttcagaaa gatcagaaac atcaggaaca acaacttctc ctcgcccgcc agaagcaaag
     6721 aatacagctg ttccgattcc taaaacagca agaatagcta ataggatcag ccaccatttt
     6781 ttctgtcctt tctttttagg atcaggcaga acttcttcct cctctggtaa ggtttcgttt
     6841 tctggcttgt cttttggcag aggcatagat ttgaatgaat caggcattgg tgcatcttcc
     6901 gtaaggggtg tgataacttt tgtttcatta tccatcacat gaggctgcca tttttcttcg
     6961 tttgcacgag ctggtgataa tactgtagaa agatcatcag acatttcctc ggctgactta
     7021 taacggtctg caggttcttt agccgttgcc cgtaaaatca cgttttccag tgcttgtggc
     7081 acatttggat caagtgcttt tatagatgga agatcatctt gaaaatgttt taaagcaatt
     7141 gttacagcag attcaccatc aaacggtacg ctacccgtca acatctcata aaggatgatc
     7201 cctaaagcat aaatatctga ctgcttcgtt gccatacttc ctctggcctg ctcaggggac
     7261 agataatgaa cagatcctag catcgtattc gtctgcgtga tcgatgtttc agataaagcg
     7321 atagcaatcc cgaaatccgt gatttttaca acaccttcat tatcgatcag tacgttttgg
     7381 ggtttcaaat ctcggtggat gatttgatgg ttatgagcta aagaaatagc agaaaggatc
     7441 tgctgcataa tatttactgc tgtttcataa ggaacaggat aatgtgtttg gatatatcgt
     7501 ttcaagtcca ttcctttgac gtattccatc accagatact gcatgttgtc ttcttcacca
     7561 acatcgtaaa cactaacaat gtttggatga acgagttctg ttgctgctaa agcttctctc
     7621 tgaaatcgtc gaatcgcagt ctggtcattt tgaaagtcaa atcgtaagac tttaactgct
     7681 acatcacgat ccaagattaa atcgtgcgcc aaaaatacat ttgccatacc accgctaccg
     7741 atatttccag tgatttgata acggccattt aattttttgc ccaactcgat catacactgt
     7801 cctccttata ttcgataaga agaacggtga tattatccgc tccgcctgct tcattggcac
     7861 gatcaatcag ctcagttact ttatgagaca atgggccttc ttgattgatg atcgttccta
     7921 tttcttcttc agacagcatg ttcgtcaagc catctgaaca taacagcagt cgatctttta
     7981 gttgccaaag atgagtagaa acatctactt cgactgttcc aggcatgccg acagaacgtg
     8041 ttaaaatatt tttccttgga tggttcactg ccatttcttc gctgatttct cctgatttga
     8101 ctagttcatt gactaaagaa tgatcctccg tcaattgcat gattttatct tcacgaatca
     8161 aataagctct gctatcccca acattggcaa tcgtgaacca gtcatctgac agagcggccg
     8221 ctacgattgt tgttcccata ccgttatatt ccggctgttc ttgtccacgc tgataaatca
     8281 gcatattttc ttcttgaatc gattgaatga accattgggc gattttttca tcgtcaacag
     8341 cttcttgttt ttcccaagct tcgcctagat tcgttaccgc catctgactg gcaatgtctc
     8401 cggcacgatg accacccatc ccgtctgcta atacagcaag cttgagacct gaacggctgg
     8461 taaatacact ggcatagtct tgatttgtgt tacgtcgtct tcctacatct gattgatatt
     8521 caatctgcat tttttcacct cattactttt tgcgcaaaca acaaatgaaa aatccatctg
     8581 tgtaaaactg atgcggatac aaagtcaaca ttttctcttc gattgatgat tgtagcatag
     8641 gattggtcaa aacatcaatc ctctcaaaat cagggtgtct ttccaagaaa gcagaaacaa
     8701 cgtcttggtt ttcttctttt aagatcgtac aagtgctata agtcagaata ccggaagact
     8761 ttaaagttgg tgcgcagctt tctaaaattt ccaattggat ttttggtaaa ttcgctaatt
     8821 catccggctg ttttttataa cgaatatctg gttttcgccg aagcagccca agacctgaac
     8881 aaggcgcgtc tactaaaatc cgatcaaact gctctgcttc gaactgttta ctgacttctc
     8941 tagcatccat cttttgagcg tagacagcag aagagacacc tagacgtgct gcattatctt
     9001 caatgagttt gactttgtgt tcgtgtacat ctaaagcgac cacttctcca ccgcatgacg
     9061 gatctaaaaa agaagcaata tgcgtcgtct tacctccggg tgctgcacag gcatctaaga
     9121 catgatgttc aggctcgatt tgcatagacg gcgctacaag catcgaactt tcatcttgga
     9181 tcgtcatgac gccatcgtgg aacaatgagc tagaagctaa atgccctttt ttcgctacta
     9241 taccgtatgg actgacttgg cttttttcag ctgtgatctc ttcttcttgc aaaacagtga
     9301 tggcctgatc tctggaaagt cggcgtgtat ctactcgggc actagcatgg ctaggctgat
     9361 atagcgataa accgagcttt tcagtttctt cgtatccgat ttgatccacc agtcgtttcg
     9421 tcatccatag aggcaggctg attttgacag aaagtctttc gataggatca gcaatgcctt
     9481 gcagatctgg tgctccttga cgctgataat tcctcaagac accatttaca aatttccctg
     9541 tcccaggatt tccttttact tttgcgattt cgaccgcctc gtttaaaata gcatgtgcag
     9601 ggactttatc taaaaattct aattggtata cagaaagata aagaagcatc ttgacccagt
     9661 catccacttt ttttgcttta gcgatcaatg gtgcaacgta aaattccaat agtcgctgat
     9721 ggctaatcgt accatagatc aattctgtaa ataaacccac atctttttga tttagctcgc
     9781 tttgtttgat catctcattc aacaaaagat tcgagtaggc gccacccttg ttcactcgct
     9841 ctaatgtcag taatgccata tatcgtactg attttttcat ttttttaggg atttttttca
     9901 ttatttcacc tgctgaccaa tctgaacttg ctgaccgctt ccgttaagga aatcatgtac
     9961 ggcctgtttt cctttaccag ctggctgtaa ttcattgatg gctaagacgg tctgtttacc
    10021 gcaagcaatc catagatttt ttttgtcttt ccggatgatc gtaccaggtg ctttgtctgt
    10081 agtttctgcc aatacttcaa ccgataataa tttccagcgt atgccctcat aggtcgtgaa
    10141 tgcaattggc caaggacgca ttccgcgtac ttggttgtca atttcttctg cagttttatg
    10201 ccaatcgatt gcttcttgtt cccgtgtgat gtttggcgaa aaagttgctt ctgactcgtc
    10261 ttgaggtact ggtttttgtc catccaaaat attgggcaat gtttctagca atagctttct
    10321 tcctaaaagg ctaagctttt caaacatcgt ccctacatca tcttgtttag tgattggtat
    10381 gctttcttgc gcatagattc cgcctgcatc catttttttg atcatttcca tgatcgttac
    10441 accagtttct ttctccccat tcatgatggc ataatgaaca ggcgcacctc cccgatattt
    10501 aggaagtaaa gaagcatgga cattgattgc ccctaatttt ggtacttgca ataacttttc
    10561 aggcaagaat tggccaaatg ccgcagtgac gatcacatca ggcaccaatt caatgatttc
    10621 ttccatttct ggcgaaccgg agattttctc tggctgcagc acacggatac cgtgcttcag
    10681 agccgctttt tttaccggtg tcggcgtaat tactcgtttc cgcccaactg gacggtcggg
    10741 ctgtgttacc actgcctgga tctcgtaacc tgattctatc agcccttcaa gaatcggcac
    10801 agaaaaagca ggtgtgccca taaatactag cttagtcatc aagatgttcc tccatatatt
    10861 cttcaagttc ttcttccggg atctgctgta tcattttctc aatgaacaag ataccgtcta
    10921 agtgatcgat ttcatgctgg aaggcacggg ctaaatagcc aaaagccgtc acttctatct
    10981 cttctccgtc tcgatcataa taacgcacgg tcacttcatc tgctcgcttg accgttccat
    11041 agacatgcgg aatactgaga caaccttcta cgtcaaggct ttcacctttt gcttcgatga
    11101 tttctgggtt aatcagttca aatttctcgc cttcatctac ttcgatcaca gcaattctct
    11161 tgttttgtcc tacttgagga gcagcgatcc ctattccgtc attcgcaatc attgtctcat
    11221 ataaattgtc taataaagcg attgtctcgt ccgtgattac atcgattggc tgagcagttc
    11281 tttttaattt atcattaggg tggatcaata taggatagcg cataatttgg ccctttctta
    11341 aataaagttt aatggttcgt tgtcaattgc cacaaaaaga ccttgtcgct gttctttctg
    11401 gcttccattc aaaatttcat ccaaaacatg aggaagattc ggttcatgtt tatatttgat
    11461 gattagttga taataatatc ggtttttcac tctcaaaatc atgctgggcg ttggccctaa
    11521 tagcaagctt tgagggctta acacttcttt tagctgattg gcaatctgaa agattttctt
    11581 tgccgctact tgttcttcag gatggctggc ggttattttc acagtaaagt aataaggagg
    11641 atagcctcca cgatgccgta catgcatttc ctgctgataa aactgctcat aattttgttt
    11701 tttagccaat tctatggcgt aatgttgagg attgaatgtt tggatcacga cttcgcctgc
    11761 tttttccgct cgccctgctc gtcctgaaac ttgggtaagc aattggaatg tatgctcact
    11821 agaacgaaaa tccggtaaat tcagggaggt atcagcattc aacaccccaa ctaatgtcac
    11881 ttctggaaag tccaaacctt tagcaatcat ttgtgtccct aataatatat ctgcttcttt
    11941 tgcaccgaat ttttgtaaaa tctgttcgtg tgcccctttt cttcgagtgg tatctacatc
    12001 catacgcaaa atcctagctt gcggataaag ttctcggagt tcctcttcta ctttttgagt
    12061 acctgttcca tagtagcgga ttttattgcc gccacaatta ggacagcgat tgggaatagg
    12121 ttcttcatgt ccgcaataat ggcagcgcat tgaacgtgta tccatatgca gtgtaagaga
    12181 gatatcgcaa ttaggacatg gaagaacaaa tccacagtcc ctacacatca caaaagacga
    12241 atatcctcga cgattgagca gtaggacagt ttgttctttt ttgtctaatc gattttgtat
    12301 tttttcctgc aaattagcag aaaaagtaga ggtccgatga ttctgaaact cttcacgcat
    12361 atctacgatc tcaatagctg gaagctgtgc agctgctttg gctcggtggt ttaactgcaa
    12421 taactgataa acgccttttt gagctcgtgc tcttgattct aaagagggcg tcgcgcttcc
    12481 tagaactatt gggcaatgat gatatttact gcgccaaatt gctagatctc tcgcatgata
    12541 acgcggtgtc tcatcttgtt tgtaacttga ttcatgttct tcatcgataa tgatcagccc
    12601 gatatttttc agcggggcaa aaatagcaga tcgtgcgcct actactactt gcgcttcttc
    12661 ccgctcaatc ttgcgccatt catcatattt ttcaccttgg gaaagtccgc tatgtaagac
    12721 cgcaactgat tctccgaagc gtcctttgaa tcgctcgacc atttgcggag tcaatgcgat
    12781 ttcaggcact aacataatag ccgttttatt ttctgacaaa acatcagcga ttgcctgaag
    12841 atatacttct gttttcccgc tgccagtgat tccctccagc aaaaagacct gatctttttg
    12901 ctgctgacct gctgtgatga tttgttcgac agcatttttt tgttctgcat tcagttccaa
    12961 tgccgtcgtt tggtcaaata ctcggtcttt ataagggtcg cgataacgtt ctgattctat
    13021 aaactccagc cagccctttt cttttccttg attcaagaca gcagtactga ttcctttatt
    13081 tttgaaataa gcaattggtt cttcttggct cagttcagat aaacacaaaa gaagttcttt
    13141 ttgtttcttg gcattttgcc gtaattttgc ccattcttct tcgatttgct tttcagttaa
    13201 caaagaacgg accaaacgaa tcgtttttac tttatttcgt gtatggactt catatttgat
    13261 atctactaat tgctcttggc gccagcgaat caattgagaa aggctgcctt cttcttctgc
    13321 ttccttccag gaaatcactt ctcgctgatc aaacaatacc tgaacttctg gtgtgtctgc
    13381 caaaggatag atcaatttat catattctgc tttcatcaca ctcggaagca tcgtttgtag
    13441 acaagtgatt ttgaatgcgt aagtaatttt tttcatataa tcagctaatg aaagtaattc
    13501 ttcattgacc accggagcaa ggtctagcaa acgaataact gccttcaaag atggattagc
    13561 tgattcttct gatttttgga tagccataac aaatccttgg acatgacgat tgccattacc
    13621 aaaagggact tctactcgca tcccgacttg caaagctgtt tccatttcag agggaaccaa
    13681 ataagtgaat ggctgatcgg tctgcatagt aggtacgtcc acaatgactt cagctgttaa
    13741 tgtcatggtt cgtttcctcc tttttttgtc ttttcttatc aagcgacaaa aagttccttg
    13801 cctattttac tagagatcga tggatttgtc ttatcttctg ccaaatccta acaaaaaatt
    13861 aacagcagat aagaaaaaaa agaactgtgt caaaaagttc agacacagcc ttttctacca
    13921 gacaataagt ggcgaatgtt tgaaaaggaa acagctgggt ttgcttttct ttttagatgt
    13981 tcgctctatt tttctttatt acattttttg ctcttcacgg atacgtgatt ccaattcgtg
    14041 ttgttcacgc tctttttgtg cttgacgttc ctcttcttcc atcttcaaac gttcacgttt
    14101 taattcagga tgtggatcat tgactacatt tcctgcatcg atttcttcta aagcttgacc
    14161 gacgcttttt actgaatcaa aagaatctag agtaggctgt gcacttgcat ccaattcatg
    14221 cgcgcgttta cttgctaaaa tcactagaga atattttgaa tttacacgat ctaatagtga
    14281 gtcaattgaa ggttttaaca tcataagact acatctcctt taacatttta atatatttac
    14341 cgattacgcg atctactcga aaatgttcac tggcaataat gtttttgatt cgttcaactg
    14401 ctagcggcac ttcatcattt acaacagcat agtcgtaaag agccatcatt tcgatttctt
    14461 cttttgcaac tttcattcgt ttttcgatta cttcatctgc atctgttcct cggcctacga
    14521 tccgtgattt taattccgct aaatctggcg gagtcaaaaa gatgaataca ccatcgggca
    14581 ctttttcttt gacttgtttt gctccttgga cttcaatctc caagaaaaca tcttttcctt
    14641 catccaatgt cttgttgaca tatgacaaag gagttccata ataattcccg acatattctg
    14701 cgtattccag catctctcca gcttcgatca ttgattcaaa ctcttctttg cttcggaaga
    14761 aataatcaac gccttcgacc tccccttcac gcattttacg ggtagtcatt gagatcgaat
    14821 actgaaaatc attatcctca ctctcaaaaa ttgcttttcg tactgtgcct tttcctacac
    14881 cggatggacc agacaacacg attaataatc cacgctctga catgacgacg tcctttcaac
    14941 tagtcttttt gtacttatcg tttattttgc actttttttc tgcaactttc aaggttatct
    15001 ttaattatag ctagaaattg gtaaaaaagc taccctgtac caagaggtcg taaaaaagct
    15061 gtcctgcatc aagtaataag aacagccaaa cataaacagc ttctcatgtt tttcgtttgg
    15121 ttggggttat tatcgcagat gcaagctttt gaacgccgtg tataagaggt cgtgaagaag
    15181 ccgattagtt tcgagtatga agaaaaattt tgtgaaaata gtttctcaca tttttgcata
    15241 aatttggctt aataccgaga aaatggtttt tgaacatcac ttattcaaaa taaaaaaaga
    15301 ttgtgacacg aattttgtca caacctttaa atgtattcct agagcaaaga agctaagcga
    15361 agaaatccaa ttctgtttat ttattgtatt tctttgttca gcatccttgc taaaagccca
    15421 tattggacaa agtattcact acacccatga tctcatcttt tcgagaacgt gctttttctg
    15481 tcggatcttc tacctcttct gtgtcaattc gaacaataaa tttccgattc atcgtcacgt
    15541 aagcaccgtc gttgatcaaa ataaacagtt cgttctcatt atagtctgca caggcatctt
    15601 tccatacgtc aaagccgttt ttttcagaag ggatatcccg tttaaccaca gtttttccac
    15661 ttaattcgtg gaacgttacg tcataatatt tcattatctc tacctctttc gtcattcaat
    15721 attttgtact tgttctctga ttttttctaa gatcgtctta gactggacga cttgattttt
    15781 aatttcgatt gcatttgatt tggaaccgat cgtgttgatt tcccgattca tttcttggat
    15841 caggaaatct aattcccgac ctacaggaca ttgtgcttcg ataagttcat ctagtttttg
    15901 tacatggatc gccagacgat ccagttcctc gtgaatatct ccgcgttcca acaagatggc
    15961 tagctccgtc aacaaccgtt gctcatcgac tttcgctcca agaaattcct ccagcttttt
    16021 ttgatacctt atttgaaatt ccttttcata gatttctaca aaactatcga gtttttttag
    16081 tacttctttg aattcttcag tattcttttt caacactaaa gctaaagctt gaccttcttt
    16141 ttctcgactc gcctcaatag aatcaagtgc gcttttcaat gtatctagag ctaactgtgc
    16201 caattcttcc atgctctctt ccggtttttc ttctaccgtt acgaagtttt cgtccagcac
    16261 gaagcgttcc gccatccgac taattgacac ttgattttga ctaccgtatc gttgttgaat
    16321 tccgtctgtc aattgagtga taagtgaatc aatcaattcc cagttgatga cgatctgttt
    16381 ttcgtttttg ccaatataag ctagattcac aaatacttcg attcgaccgc ggcttaactg
    16441 ctgtttaata agctgacgaa aatcgttttc aacaaagttc aggattttag gacttcgtat
    16501 ttgaacatct agaaaacgct ggttgacact ttttatttca atatctaact gatacgcttt
    16561 tgtttcgcgg atggctttgc caaaaccagt catacttttc attcgacagg gtccttcctc
    16621 attacatatt ttctattgct ttttgcagtt gttcaaactc ttcgttcgtc aatgtcaggc
    16681 ctttccccat tttttcatgg tctggcgccc aatctcgaag atcgaatttt ggcgggcgac
    16741 cattccagct gactaggttc agttctttac gccacccctt gttgttttct gacaatatag
    16801 cgatttcttc tacgatttca tatgaaaatt cttgtgccat ggttgttcct ccttgagata
    16861 atataaatat aggatcagta atgtgtcaac tggttgctta agcaattcat gctgcaaacg
    16921 atccttgcgt tttccttgta gcagataatc aaaataaaca gttttcgtgc gctcattttg
    16981 tattttttgc tggtattttc gataccttcg ccattcttca gtacttaatt gaccaatttg
    17041 tgccaataaa tcatccggca tactttttat atcttgttgg cagatcattt gaatcatcat
    17101 gtctacttga tagatttcat tgggcagtaa aaaatgtttt tttgctaaaa gttctagcat
    17161 cgccaatccg catgtctctg gtacgaccct gttcttatat aaattacgca gtttttgcca
    17221 aaattttatg cttttgaaaa aagataattc taactgaatt tcttttgtta agtaagacaa
    17281 aaatcctcgc cagtatgttc ttgcaggttt aacagaatga aatgtatacc agttcccata
    17341 gtaatagcct aatcgttttt tcatttccag atagcctaat tgtaaattta attggttcct
    17401 ttggctatca aaaagcagaa agctgcctag tgtccacaat gtttgacatt tccaatggat
    17461 aaaaatgtct ggcatacgaa tttttttcgc tgggcctggc ccttgcacat ctacgacaaa
    17521 tgcctctgtt gctcctttgt taatcgcaat atcaataggt attttgttgc gatacccacc
    17581 gtctgcatat ttattttttc cgatctttcg ataagccata gcgggataaa atgaagcaga
    17641 ggcgagtatc caatctggta tttcttctgt ttttgtctgt tggatatcag tcactacttc
    17701 cgctagttct ggcagtttag tagacaccgt atataaacgt attttgggat tatgtttgag
    17761 taaggacaaa tccaaattgt ctttgaccat cttttccaat ggagcagaag aaaccccgcc
    17821 ttcaattagt gctgttttcg tcatttggcg tctttctcga tagaagcgtt cacgaaggtc
    17881 ttcaactgca gccatttctg gtaacgcaag cacttgtctt gtcgacagct ttttccataa
    17941 attcacagct tttttcatat ctccttgtaa aatcaatgcg ccgttcaacg cacctaccga
    18001 ggtacctgtg ataatctcaa agttgatatc atgttccttc agcgcctgcc acacaccaat
    18061 ctgataagca ccgtgcgctc ctcctccacc taaaacaagt gctgtatgat agaccagctt
    18121 tttatttaat tgtgtggttc cttgatagcc gtttttatga aaaatacgca accattcttc
    18181 tgatagagga gattgaaata aaaaacaaca gttttcttta ataaaaaaac gagcgcaagc
    18241 ttccatatac cgaaaaaatt ttttccattc aaaagtttca ccttcaatca acagattatt
    18301 aaaaataaca tctcctctac ttttagcaat agtaaaatag acggtatatt ttttcgatac
    18361 tgctttaaaa gcacattgct tttttgcaga acgccaaaaa gcgtctctac tttcttgatg
    18421 actttgaaaa aatggaaggg gttgttttgc ttgctggata ggcgaataca gcgacatttt
    18481 ctgtgaaaag taaacttttt ttatctccat cgcgctctcc tttttcttca agtataccag
    18541 tacgtcataa aaaggtcatg agtctcgtat tcgactttgt agattccata agaaaacaat
    18601 tcataaaacc tcctttaaat aaaaaaatac aaaaatcaca aaaattaatt gaaaatgaga
    18661 gtgttttcaa tgaaaacgtg attttgtaat ggaattttaa aataaaagaa acaagctttt
    18721 ttaatgcaaa tgttgtaaaa taaaagagat taagaggaaa taagaggagg aacatgatga
    18781 ctgagtcagt ttatgtacat attgatacca caagtaatgc tgttttaaca aaagggctga
    18841 ctgctactga ctttgcaaac agtatcgtgc attttcctca aaacttgctc ttgcttgatc
    18901 catctgcttc tgctggagaa tatgaatccc atacagggct gaaagtaatc cgtggtacag
    18961 aaaatattca gcgtttcttt tcctcttctc gtaacaggcg tggattcgat gaattgaagt
    19021 ggatcgactt tactgattta acgatgctga aagaattgac acctttagaa atttcagaat
    19081 tattatattt tggtcatatg aaaacacatc ttcattcgcc gtttttttat aaactacaga
    19141 ataattttgt atacttcgat cttaatgatc agctgaatcg gatttattat cgctatttag
    19201 atgaatttta taggattttt gctagtaaat taagttcgat cttgtcagat aagatcaatg
    19261 taaaaaaatc ttttttcaaa aaagaaacac ctgttgaaaa aatcagtgtg gaattattga
    19321 aagaaatgcg taatttgatg caggaaggga tcattttttc ttttgatcaa gttggtttga
    19381 tcgatggtaa atatcgtatc ccaatctatg tgattgaaga ttatatttgg aaattaaaag
    19441 gcacccggta tcgagaagag gaaatcatcg ctactttaat ctactctgtc accgatcgac
    19501 actggcaatt ggtagaagat gccgatcttt cttatctgcc attgaagtag atctatttct
    19561 tatcaaaaaa taaaaaggaa acaagaaact aggacatgat tctgtctcaa gtttcttgct
    19621 tcttttttat ttacagctcc tgtcgtttat ctggcacaaa aatcgaagga gtcgtttgtt
    19681 ggatccctaa taaaacgatg atcgttacaa tagctgtact caacccgctg gcaccattca
    19741 ttacgaaaga aaagagccaa ggattcatcc cccatagtgc atacgctccc cagaaaacca
    19801 ctcctgcaat aaaatgccaa aaatatctgg aaagtgcgcc aatcaatgca ccaaacacga
    19861 tgctttttct aatttgtttc gtattatcgt ctacatagcc tttttggaca tttgaagcca
    19921 tgagccctgc tgcgcctgca aaagtatagg caatcgggta ctcaatcaac acttgaacga
    19981 cacttaaata ataaacttgt cccaacggaa aatgtaggat gccccaaatg aaacctgcca
    20041 gcatccctgc ctttgtccct cttcttaacg caaaaagagt caatggaatc tgccctaatg
    20101 aaatagaaaa actgctgccg atctggatcg gaatcaacga caacaccatc gctaacgctg
    20161 caacaatcgt tccttctacc caaactctat tttttcccat aaaaaaactt cctcctttga
    20221 ataaaaaagt cacaaaggag gaagcagctt tcgaaaatag tcaaacagct gtttattcac
    20281 aattcctacg ctcgcataac cgatacaggt acgaagggtt tagaaataga ttcaatctca
    20341 gcccaattgg actccccttt gtgagtttat ttattattta ttttttgttt caaacaatgg
    20401 actaactggt ttgttttcat ggatacgttt gattgcatct cccattagtc cgccaacact
    20461 tacttcatcg attttgtcga ttttacgatc ttctggaaga tagatcgagt ctgtaacaac
    20521 tagacgctcg atagctgaat cttctattct ttgtaaagca gggccggata atactggatg
    20581 tgtacatgaa gcatacacac tagttgcacc agcttctttc aaagcattgg cagccaatgt
    20641 gatcgtacct gctgtatcga tcatatcatc gatcagtaca caagttttgc ctttgacttg
    20701 tccaatgata ttcattactt ctgcaacatt tgcttttgga cggcgtttgt ctatgatcgc
    20761 aatcggagat ttcaaaaatt ccgctaattt acgtgcccgt gtgacaccac catggtctgg
    20821 tgaaacaacg acaacatcat cgcctttgat tcctttttct aagaaataat tggcaataag
    20881 tggcgcaccc atcaaatgat cgactggaat atcaaagaaa ccttggattt ggactgcatg
    20941 aaggtccaaa gtcagcattc ttgttgcacc agctttttga atcatgtttg ctactaattt
    21001 cgctgtgatt ggttcccgtg cacgtgcttt acggtcttga cgcgcatagc cataataagg
    21061 catgactacg ttgattgttt ttgcacttgc tcgtttcagt gcgtcaatca tgatcagcaa
    21121 ctccatcaag ttatcattga ctgggctgct cgttgattga ataacataga catgcgatcc
    21181 acgaatactt tcttcaatgt ttacttgaat ttctccatca ctaaattgag taacagatga
    21241 ttttcctaac tcgacgccga ctgctgctgc aattttctct gctaatgggc gattggaatt
    21301 taacgcaaaa atttttaatc ttgggtcaaa gtaatgtttt gacatgggga cctccacttt
    21361 ctttttcaca actctagaat tctacctaat aaatagtagt catatttttt aaataaatca
    21421 agtgattttg caaaagtctt agtttagata agggagtttt tttgcatatc cttctttatt
    21481 gacttgtctt gctctcgcaa ttgccagcgc atattctgga atattgtctg taattgttga
    21541 cccggcagca atcgaactgt ttttagctac ctcgacaggt ccgatgatat tcgtgctaga
    21601 accaataaag ctgtggtcgc cgacagtcgt atgatgtttg ttctttccat catagttgac
    21661 gaagacaaca ccacatccga cattgatttc ttctcccagc gtagcatcac caacataagt
    21721 caagtgaccg actttagttc ctttgccgat ttgagcattt ttcacttcca caaagtttcc
    21781 gatatggacg ttttcgccaa tttcagcttt tggtcgtaaa tgtgcataag gtcccacatc
    21841 tgcatgtttc ttgacatggc tgctttcaat gactgagttt tcaatgacga cgtgatcctc
    21901 aatcctactg tctacgattt ttgaatgtga cccgatgaca caatcagaac caatgactgt
    21961 atttccttgg atttgtacac ctgcttcgat caatgtgtct ggaccgattt ctacgcctgc
    22021 atcgatgtat gtcgttgctg aatcgacaaa gcttacgcca ttgaccatat gcatctggtt
    22081 aattcgcttt ctcatgatct catttgcttt agctaacgcg atccgatcat tgactcccat
    22141 tgattcttca aaatcatccg tttgataagc agcaactgta tggccttctt cttttaagat
    22201 ttcgatgata tctgttagat agtattcacc ttgagcattg tttgttccaa ctttgcttaa
    22261 cgcatcgaac aaagcttcgt tgtcaaaaca gtaagttcct gtattgattt cttgtacaag
    22321 agcttcttct ggtgttgcat ctttttgttc cacgattttt tctacgatcc caatatggtc
    22381 acggatgatt cgtccatatc ctgttgggtt ttctgcttgt gccgtcaaga tcgttgcact
    22441 tgcatttttt ccttggtgat attcaaaaag attgttcagc gtttcagtag tcaataatgg
    22501 tgtatcgcca ctgataacta acgttgttcc tttttttcct tccaagaaag aagcagcttg
    22561 caaaacagca tgtcctgttc ccaattgttc tgcttgtaac gcatatttac tgcgttctcc
    22621 tagttgtgct ttaacttgtt ctgctccgtg tccaacgatc gtaatcactt catctggttt
    22681 cgtttcactg acccgattaa tgatatgttc taccataggt ttaccagaaa cgggatgtaa
    22741 aactttatat aattttgact tcatgcgggt acctttccct gccgccaaaa taattgcata
    22801 acgtgcgtcc aactgcgtca ctcctaaata tagtttgctg atttcagttt atcccagtat
    22861 acccattttt tcaacaaaac aagtgatttt cttttatccc agcgttttct ggataaaaaa
    22921 atagatagac cataacgggc ctatctattt ggtaaccggt gctctcctgc caactcctcg
    22981 gtcacaagtc acactactca taaaacatga aaagctgttt tttgaaatcg tttcttgtga
    23041 cttagagtta aacggtaggc ttacaacctc ttcattaaac gtttttcaat cagctgacct
    23101 tcggtattag ctcaataaac ggcaaaatat gaggagcaat ttttccgttt attgagctaa
    23161 tactcaggac aggacgctga tttcacaacc tctagataaa cggtgttcaa aagccagttt
    23221 ctcggtatta agccaaattt atgaaaaaat gtggaaagcc attttcacaa aatttttctt
    23281 actactcgaa actaagcggc tttttcacaa cctcttattc tgaaaaataa tttcctggca
    23341 caaccgtaat atttttcgtt tgagtatcta catctttgac ataaagcagt gacgtataat
    23401 catcgatggc gcgatgaccg ttgaattttg attcggcaaa tacagtgatg ccaacaagtt
    23461 ctgattcgaa ttcttcaatc aagctcttca ttccgttgac tgttccgccg cctttcatga
    23521 aatcatccac tacaaggaca cgtgaaccac gtttcaaact tctcttagac agttccatct
    23581 tttcaattct ttcagaagat ccagacacat agttgacact tactgttgat ccttctgtta
    23641 tttttgagtc ccggcgtaca ataacgaatg gcgcattcag ataataggac acagcttggg
    23701 caataggtac gccttttgtg gctacagtca ttacagcatc gattttttca ccaaggtatt
    23761 tagaagcaat gattcttcca acttgacgca gcagttcagg ttctcctaac aaatcagaga
    23821 gataaacata tcctcctgga agcagacggt cttgttctga tagacgttct gctagagatt
    23881 caatatattt tttcgcctct tcatatgaaa tttcagggat gaacaataca ccacctgcag
    23941 cacccgggat tgtttccaag gttccatacc ctcgttcttt aaatgttttc ttgatgattc
    24001 ccaaatcttc actgatagaa gatttagcag attcataacg ttcggcaaag cttgttaagg
    24061 aaatcaattc atgcgggtga ttcaataaat actgtgtcat gtcaactaga cgttcactgc
    24121 gacgtacttt cacctattcc aactcctttt aagaaatttt atcgttttac attatataag
    24181 tttttcttca aaaattcgag cattttatga aaaaataaga aaatagttcg gtatttatta
    24241 aaataaaaag aagcctataa aaaatttttc gtttttataa ggcttctttt ctttataatt
    24301 tagttatttt ttcttctgaa ggcaaccaat attcgtttga tgatatttac aatcaggaac
    24361 aaagcaataa agactaatgt gattgttgct cctggaggtg tatctagata aaacgagcta
    24421 gttagtcctg acagcatccc tagaaatcct acacaaacac tgatagcaat cacgacattg
    24481 aagcttttgc ctaatcgcat accgattgct gcgggaagaa ccatgattgc ggatatcaaa
    24541 agtgcaccgg caatcgggat catcacagca atagccacac cagtaatcac attgaacatc
    24601 atcgacatca aatgaacggg tagcccatcc acgtgggcgg tatcttcatc aaaagtcaga
    24661 acatacatcg gccgtttgaa taaaaagaac aatataccga tggcaacgaa taagattcct
    24721 aaaaagatga cttgctccca tgtaatggtc acgatcgaac caaacagata ggattggata
    24781 cttgttgctg aattccctcc gctcaaattc atcaatacta acgctagcgc caaaccacca
    24841 gccattaaaa tggcaatgga gatttctgaa taggttcggt agatcgttcg cagatactcc
    24901 aatatcacag ctgctaatac aacgactagc atggtcgtaa gtgtaggatt aaggttcaaa
    24961 aagaatccta acgctacccc tgctaaagag acatgagaaa gcgtatcagc catcagcgat
    25021 tgtcttctga tcactaaaaa cacacctagc atcggggcga ttccggcaat gaagatagcg
    25081 gcaagaaacg ctcttctcat gaattcatat gaaagcaacg ccattgagaa tcctcctttc
    25141 tcaccagtcg gatctgtctg tccgcatact gtttgatatc ttcatgatca tgggtgatca
    25201 ttagaactga tttcccatgg acatgcgcac tatgtcggag aagacgataa aattcatttc
    25261 tggattcttc atccatacct gttgttggtt catcaagaac aaataagtca gggtcagtag
    25321 caaaaatacg cgctaaactg attcgctgct tttgtccgcc agacaattca cctattcgcc
    25381 gatttcgcat atcccacatc cctacagctt ctaaagcacg ctgtacatgt tgatgatctt
    25441 tttcagagag acgcttgaac cagcggcctc ttggatatct tcctgaacgg actaattcga
    25501 tcaccgtact aggaaaacct gcattgaaag atgcaacttg ttgaggaata taaccgatac
    25561 ttaatttttt tccttcttga tttttctcag caatctttat cattcctttt gaaggtttca
    25621 gtaatcctaa cgatgctttc accaaggttg acttggctgc tccattttct ccagttaaaa
    25681 tgacaaattc acccgcatct acatgataag agacatcttc tagtacaggt tcatcatcat
    25741 agtaaaaggt caaatcagat atttcgatat aacgcacctt acaacctcca ttctaaaact
    25801 taattgatac tttcttttaa agctttcaga tttttttcca tgacagaaat ataattttcg
    25861 cctgctttga tttgttcatt agtcaagctt tctaaaggat tcaacacttc tagcttcact
    25921 cctgtttcat tcgacaatgt ctcagctacc ttcgaagagg cattttcttc aaaataaatc
    25981 actttgatgt tgtttttctt cacaaattct tttagctcag ctaagcgact tggtgtgggt
    26041 tcctgatctg gtgaaacgcc cgaaatcgct acttgtttta agtcgtattg attcgctaaa
    26101 taagcaaaag cagcatgctg agtgacaaat gtgcggtttg ttgcatcttt tagctcatcg
    26161 gagtattttt gatccagtgc ttcaagcttt ttgatataag catcggcatt cttcgtaaaa
    26221 gtttcctttt tatctggata tttttcgcct aattcttcag caatcgttct gacttctttt
    26281 atagctaata ccggatcgag ccaaacatgt ggatcgtatt catgtgaatg accatgccct
    26341 tcttctgatt cttcatgttc gtctgcttct tgcgattcta aacggtcgat tcctttagct
    26401 gcttcgatca tcaaggtctg tttgctatcc actgaatcct ttaacgaatc cacaaaaaat
    26461 tccatgtctg aactattata cacgaaaaca tctgcatcgg aaatttctgc tctttctttt
    26521 gcacttggtt cgaaatcatg cggctccgtt ccagcaggga tcaaaagttt cacgtttcct
    26581 tcatccccta cgatctcttt tgtaaaatca tacatcggat aaaaagtcgt aacgatcgtc
    26641 aaatcctctt ttttgtctgc ttccttgttc gtatttccac atgctgcaaa cagtaaagca
    26701 cctatcatta ccgtagctgt caaaagatat tttttcattt atatgctcct ttcaatgaac
    26761 aaatcggaat tttacgattt attaacgtga actaatgtat caaaataaaa aaacacagtc
    26821 aatagaaatc catcaatttc tgatttaagc atgtatattc ttgattaaat cgtaaaatgg
    26881 atgagttgct gctcatatct agccatttca ctacaataaa aaactgaaga ataccatttt
    26941 aggattcctc agtttttcat cacctatttt acttcttata ttttattgca gggttcgtac
    27001 taaataaact tcctcacaaa aaccttttaa cccattatat actctctgcg ctcttgaata
    27061 atgttgacac aaggcaaaaa cggttggtcc gcttccactc atcaatgcag catctgctcc
    27121 gtacttgatc atacgctctt tgatttgatg gatgactgga tgccgctttc ccgttatatc
    27181 ttctaaactg tttcccattt ttttgatcat ctgctgataa tcttgctctc ggatggcttc
    27241 agcaagtccg tggatgtctt gatgttggat ggcttgcgta tctacttctc taaacactgt
    27301 acgtgtagag acactcattc ttggtttgac caacacaacc cagcactgtg gcatagaagg
    27361 taatacttgg atttcttccc ctttgccacc gacaaatgcc gtacttccgt agacacagta
    27421 aggaacatct gtaccgactt ccaaaccgat ttctgccagt tcttctaacg aacagcccag
    27481 attccataat tggttgatcc ctcttagagc agctgcacag tctgtacttc ctccgcctaa
    27541 tccagctgct actggaatat tcttcttaac aaagatcttg acaccggttt tgatattgta
    27601 tttatttttt agcaataaag cagcttgata aacattgttc cgttcatcta ccggtaaaaa
    27661 tgctttattt gtttctacaa tgattttgtc ttctgttatt tcttcaaaaa atagatgatc
    27721 tgccaagtct acgctcgaca tcaccatttc caactcatga tacccatctt gccgtctata
    27781 taatacatcc agtcctaaat ttatcttggc tggcgctttt tcaatcaatt ccatcctctc
    27841 acccattcga aaagtctaag tcgcaagtat tctatcatag ggaaaaataa aagtatagct
    27901 tatgtctgcc tagtggttga ttttgtcatc gtttcaacag ctttcacaga attttctctt
    27961 cataatccaa acaagttcta gtgattctta taattctgtg agcacttctt ctgccaaact
    28021 cggaacattt cctttttctt ttcctatcag actgagtagc tgcatggtag caaatccgaa
    28081 attacttcct ttcctataag caaaataacg tccttcccaa tgatcgacat attttaattt
    28141 cttttcataa gcagactgga aggaatagaa aaaattacca taattatata cgatatgcat
    28201 gactttttct tgaaagaaag aaaaatctgt ttctccaaca ttagctaatg gagctaagcc
    28261 tagattggct tccgagtagc ccattttctc gtatacaata aacagattgg ctaacagata
    28321 gtcagctaat tcctcagatt ctttaggtaa gacccgcaac aaatcatatc ccgcctgctt
    28381 cttcgtcatc ggctgagcgg tgatgaagcc aacgactttt tgatctttac ggacaatacc
    28441 gacaccgcta gataatacat atgatttatc aaatcgtccc acagagaagt atttttctcg
    28501 tgcaccttga agccattttt gtgaaacttc atttacagct tgatgtaatt cttccggcag
    28561 ttgatcgtac caagtaaaat ggtatccttc tttcatcaaa cgttgatatt caagttgctg
    28621 actcagccga gacaagtctt ctgttgctag atcaacaatc cctgcttcac ctactttcgt
    28681 gaagtgaaaa cccaattcat gaagcatcac tacgtagctt tctgatatct tataaaacgc
    28741 aagctgatag cctaaacaat ctgcttgttt cataaactga ttcgttgctg cccgcatcat
    28801 tttaggattg ccaactggat ctcctaaaac aaagcatttg tttcctttga tctgataacc
    28861 aaaaacgact tgatcttggt cgtcttcttg gtaataaaaa taactgtaac ctgacaagcg
    28921 aagctgatgg cttgtagctg tgcctccgta tgtttccaat aaacggtcaa aacggtcttt
    28981 ttcgtactca ctgccaagct tcgtagtatc ttcagctaaa tattgataaa gcgccactaa
    29041 agctagcaat gagatgccca aaccaataag ccctgagaac cacacatcat ctgaaggaaa
    29101 caaaataaaa tgatttcctg ttattcgacc gactaaaaag cgtccgctgt gatagcctgc
    29161 aattgcgtaa acgataaaca aaaagccgaa caagctagca tcaaagatta tagctcccca
    29221 agaataaacg aattttgttc gataaaattc ttttctggat aaccacacag ctaacagaat
    29281 caagacatat accaatatca actgccaaga taacgtacgt gtgattgtat ttaaaatacc
    29341 aaaagatagt aaagcaatcg taggcagaaa tgcctttttg acttttactg aaagtgctct
    29401 agcaaggccg agtaataaaa agccaaccaa tagattcact gtctgatcta aaaaattgaa
    29461 tgagaacggc aataatactt gaaatagccg actgattgca gacagattag tgattgttga
    29521 tagaagtacc atcatgatcc ccgcaaaata gacagcactt acgagcacga tatgcgcaac
    29581 cttttggaaa aacagtcgcg gaatgttatc taaaaatcga ttgattttga ctcctgtatg
    29641 tgtgatgaac aatagaagtc ccgtcataaa aggtagaaca taataaaaca accgataata
    29701 taatagccaa actactgcta catcttgccg aacagacaat tgactcattc ctaagatcat
    29761 caaaacgtca aaagtcccca ttccacctgg gaccatcgtc agcattccta tcaatgtagc
    29821 aatcatgaac ataggataga tcgaaagcat cgaaatatct attctcatca acttgccgat
    29881 gatcaaaaat accgcaaggg ctcccgtcca ttgtccaaag gatgccagta gcagaccaag
    29941 caagcctttt ggataaaact cgaagaacaa tgtctttctc tttagataag caaaaaagaa
    30001 aatgactggt gctaaaaaac taccgccgaa aagccagatc caatagcttc gaaaaatact
    30061 ttctgactga ataaaaaaga catcaataaa tgtaagaaac gaccatacag acaaaccagt
    30121 cagcataaat agtgcgactt ttgatacagt ggctaaaacc atttttctat ttgtctgctt
    30181 tccataaaaa ctagcccgca gacttgctcc taccacacca ccaaaaccgg cgagattgtt
    30241 gatcgtgttt gttacccacg cagcaagaag aaactctttt ctttccattt ttggttttcc
    30301 ttgtttttct aagactctga tcgtcaccca atcatacaac aacatcggag tgacacccag
    30361 cagtccagcc agaatcatga gcaggatcgt catgcggctt tgttgtccca tcctttgcca
    30421 aatatcttgc caactcatgc cattagcaat atttgcgact tgatttgcca caaagactaa
    30481 cacggagccg aaaaaaatca gttttaaaat cagactatgt tcctttatcc attgcagcat
    30541 caagtatcgt ttatttttca cattttccca ttcctaactt gtatactttt gtgtcatacc
    30601 ccaatgttat cttatttatt ttaatttttc cactttagac ctgttctttg cttgcgatga
    30661 tagaaaaaga aagatatcct aatgctaaag gagaggataa catgaaagta ggatttatcg
    30721 gcacagggaa tatggcacaa gctattattg atggtttatt aaaaaaagga acagtctcga
    30781 aagaagaggt gttcgtttcc agtggacatt ttgaaaatgc acaaaaattt gctgctgaaa
    30841 ctggcgtcca tgcatgcaaa agtaatcagg aagttgcatc acaatcagat atcgttcttt
    30901 tagccgtgaa accaaaaatc attgaaaaag tattggaaga actaagagaa tatagccaga
    30961 aagtcttatt catttctatt gcttcaggaa aatcattaat agatttagaa acagcgcttg
    31021 aagatcctca ggcctccatc gtacgtgtga tgccgaatgt caatgtcagc gtaggagcag
    31081 gcgtttctgc gatttgcaaa aatgaagctg ccacggagga acagcttcag caagctacga
    31141 tgatttttga agcaatcggc agcgtctatc ctttagcaga atcagatttc agtacattca
    31201 ttggccttgc tggcagctca cccgcattcg tttacatgct gatcgatgcg atgggacgga
    31261 ctggtgtact gaatggtttg tccaaacaga cagccactga gattgctgca aaagcaatta
    31321 tgggttcttg tcaaaaattc ctttcaagtg ataaaaatcc atgggaactt gtagaccaag
    31381 tttcttctcc tggcggtaca actgttgcag gtgtagttgc cttagaagaa gcaggattca
    31441 tcccagcaat catcaaagga atcaatacga cgatcgagaa agatcttgag atgttgaata
    31501 aaaaataatg atagcaatgt tttttcgtgc cttacaaaaa aagaagagct gtggcttttg
    31561 ccgcggctct tcttttgtat tttttattat ttagcataat cagttgcacg agtttcccga
    31621 ataacgatta ctttgatgtg tccaggataa tccagttctt cttcgattcg tttacgaata
    31681 tcacgtacta aacgaacagc gtctaaatcc gagatttctt ctggcttgac cattacccga
    31741 acttcgcgtc cagcttgaac tgcgtaactt gattctacac cggcgaagct gttcgaaata
    31801 ttttccagac ttcttaaacg acggatgtag ttttctaatg attcacttcg cgcaccaggt
    31861 cttgctgctg agagcgcatc tgctgcagct acaaggacag agataacaga agtagcttcc
    31921 acatctccat gatgagaagc gatcgcgttg attactactg ggttttcttt gtacttggca
    31981 gctaattccg caccgatctc aacgtgagat ccttcaatct catggtcaag tgctttccca
    32041 atatcatgga gcaacccggc acgttttgct aattggatat cttcgccaag ctctgctgct
    32101 aagataccag ctaattttgc aacctcgacc gaatgcttca agacgttttg tccataacta
    32161 gttctgaatc gcagacgtcc gatgatcttg attagatctg gatgaagtgt atgagcaccg
    32221 acttcaaacg ctgcctcttc cccgtattcg cgaatacgtt catccatttc tttacgagat
    32281 ttctcgacca tttcttcgat acgcgctgga tggattcgtc catcttgtat cagtttttcc
    32341 aaggtcatcc gtgcgatttc tcttcggatt ggatcgaatc cggaaagaac aactgcttcc
    32401 ggtgtatcat cgatgattaa atcgatacca gtcagcgtct caagcgttcg aatattacga
    32461 ccttctcgtc caatgatacg acctttcatt tcatcattcg gaagggtaac gactgaaaca
    32521 gtcgtttctg aaacttgatc tgctgcgcag cgttgaatcg ctaatgataa taagtttttc
    32581 gctttgcgat ctgcttcttc tttggcacgc tgttcagatt cttttaccat tagagttaac
    32641 tcatgattca gctcttcttc agtcgatttc atgatgacat ctcgtgcgtc ctcttttgat
    32701 aaaccagcaa ttttttctag ttctttttgt tgatcttcaa tcattttttc aacgtctttt
    32761 tctcgctctt caattaattg ctgttttgaa ctaagtgctt cttctttact ttcaagtgag
    32821 ttctcgcgtt tttctaacga gtcatcttta cgattcagcg tttgttcgcg ttgaatcaaa
    32881 cgattctctt gagattttaa ttctaactta ctttctctca gctcactttc aacttcagaa
    32941 cggtatttct gattttcttc cttagcttct aacaatgctt ctttttttag agtttctgct
    33001 tctttttttg cactatcgat gattccagca gctgatgatt gtgcaccagc tatttctttc
    33061 tcgtgacgtg actttgcgac cataaaccct aaaccaagac cgacaattaa accgatgata
    33121 gcgaggagaa tgttgaatac cataaaatcc acctccatac tatctttttc atttttatca
    33181 tctatacagc ttctatgata aattattcta aactcgtcaa tatgcatctt cgcatatatg
    33241 tgtacaaaaa acatgcacaa ctttattcta atgtttgtac cccgtagtgt caactttaaa
    33301 taaacaaaga aacggctttc tacttattca ttaaacgatg gtttttaaaa taaatgacac
    33361 tagtttcata taggtaaacc tttaaattga gaaacttttc tattaaatta ttctttgtag
    33421 atgaacagat atttttattt tatgtaccca ctcgtataat taaataactc acgatttctt
    33481 tattcaataa aaaaacggca ggtcaaggag agtacctact cttcagacca gccgtttttg
    33541 tttatcataa tttttattct tctactaaag gcaattcttc ttgtcctttt tcatcttctg
    33601 ggacagcgac ttcttcacca ataccataag ctgctctaac tagagcagaa acttctgcca
    33661 tcatttcagg atgattagcc atatagattt tagcgttttc ccgtccttga ccaatccgat
    33721 cttctttata agaataccaa gcaccgcttt tatcaacaat gtctttttct actgccatgt
    33781 ctagaagttc tccttcttgt gagatcccta aaccatacat gacatctact tcagcgattt
    33841 tgaaaggagg tgctactttg ttttttacga ctttgatctt tgtacggtta ccaacaatat
    33901 ctgtaccttg tttcaattgt tctgcacgac gtacttccaa acggatcgtt gcgtagaatt
    33961 tcaatgcgcg tcctccagga gtgatttctg gattcccaaa catcacgcct actttttcac
    34021 ggatttggtt gatgaaaata gcgattgttt ttgttttgtt gatcgaacca gagagtttac
    34081 gcaatgcttg agacatcaaa cgtgcttgta acccgacatg tgagtcaccc atttcgccgt
    34141 cgatttctgc tcgtggaact aaagcagcaa ctgaatcgac tactacgata tctacagccc
    34201 cacttgatac taaagcatca gcgatctcta gaccttgttc tcctgtgtca ggctgtgaaa
    34261 gaagtagttc atcgatattc acacctaatt tttgtgcata ttgcggatct aacgcatgct
    34321 cagcatcaat gaaagcggcc gttccgccat ttttttgtac ttctgcaata gcgtgtagtg
    34381 caactgttgt tttacctgaa ctttcaggtc catatacttc gatgatacgt ccacgggggt
    34441 aaccgccgac acctaaagcg acatctaacg caagagagcc acttggaatt gtagagattt
    34501 gttggtcgat tttttcgccc aatttcataa tagagccttt accatagctc ttttcaattt
    34561 ttttcagtgc agcatctaag gctgctttac gatcatctgc caatacgata atcctcctta
    34621 tatttacaaa tcagagcaac atttcattat ccttatcata tcttttttag tgaaaaaaag
    34681 caagaaaaaa ccgaacaaat attcgcattt atcattttct taaaagctgt tttctcaaaa
    34741 gatcaaatcc ttgcataacc gcattatatc tgatagcagc acgatctctg gcaaagtgat
    34801 atttttccga aaccacgccg tctttagatg aaatagcgat ccatactgtt cctgcagatt
    34861 gaccttctaa cgtgtccggt ccagccactc cggtgaaagc tacactgtaa tctgttcttg
    34921 ctatctcacg tgcttgaatg gccatctgct ccacacattc tttgctgact gttccatatt
    34981 ctgctaatag ctccggatca atctccagaa aaccagcttt tgtttggagc gagtaggtaa
    35041 cgaatccgcc tggaaatact tcagatacac cagcaatgtc gcctaatgcg gcttggaagg
    35101 cgccggctgt caaactttcc gctgctgtta cagtctgctt gttttctttc agtagttcca
    35161 ctacgacttt tgctagcgaa ttatcatcgc cgtatccata aaaatattcc cctactcgtt
    35221 cttggatttt ttcttctaat gctaaaagag cttgattccc tgcctgaaca tcatttgttt
    35281 tcactgtcag tcgtaatgtc acttcatttg gtttggcata tggtgcgatc gttggattga
    35341 tctgtgtctc aatcagatct tttagctcag tgaccagtcg cgattcacca atcccataaa
    35401 agcgcaaaac tttagaaatt agtttttctt cagaaggaaa acgttcctct agtaaaggac
    35461 gtacttgttc cacaaacatt ggtttcaact cattaggcgg accgggaaga aggatatacg
    35521 catgtgtatc tgtttgataa aaagtcccta aagcaagccc tgtacgattt ggtagaggaa
    35581 ccccgccttc gataatctgg gactgttgca gattgttttt ggtcattttt cgatgggttg
    35641 tttcaaaata tgccaataat tttttgtatc cttccgtatt ttgtatgaga gacttaccga
    35701 ggtgagccgc agttgcttct tttgtcaggt catcttctgt cggtcctaat ccgccacata
    35761 agagtatgag ttcacttctt gtttcagcta gagcaatcaa ctcttctaaa cgctgctgat
    35821 tatctcctac aactgtttga taataaactt cgatacctag atcagctagc tgttctgaca
    35881 agaaagtggc attcgtattc acgacttgtc ctagtaaaag ttctgttccg acagctatta
    35941 tttctgcttt cattacttcc cgctccctta tatagtatac gctgtatttt tatatttttt
    36001 tatttaaaaa gagggcaagg agctgtgaca aaaatcttgt cacagctcct tgttccaaat
    36061 aaacagtgtt ctcctgccaa aacctcggtc ataagtcaca atatacataa aacatgaaaa
    36121 gctgtttttt gaaattatcc cttgtgactc agagttagac ggcaggctca caacctctcc
    36181 actaaacgat acttacattg atcctttgaa cacaccgctg tttttgatga agtaatccac
    36241 acctgaatag atagtaaaga tcagacaagc ataaagcatg atttgatcta ctggaatacc
    36301 gatcaggctg aatgggatat tattgataaa cagcaagata atagcaacca tttgtgtagc
    36361 cgtcttgact tttcccggcc aagctgcagc catgacttct ccatgttcga caagtagtaa
    36421 acgcaagcct gtgacggcta attcacgaca gacaatgatc gctactaccc aaccaggggc
    36481 ctttccttgg tcaaccaaca tgataaaagc agtcataact agcattttat ctgccaacgg
    36541 gtccgcaaac ttgccaaaat tagtgacaag gcctcgcgca cgggcaattt ttccatctaa
    36601 ccagtctgtg atactagcta aagcaaaaat aactgcacct actagctgtg tgacagccag
    36661 tgtggtgtct cctacgttaa attgtcccca atctaaaggt aaacctacaa ccaaaatgaa
    36721 aatcgggatc atacaaattc taagtacagt taatttattt ggtaaattca cactatcgac
    36781 tcctttcttc cctattctac gtgactatca gcgaagtttc caccgatatt atttactttt
    36841 ccaaatgaat ttattcaaag gagtattttt attgaaattg atctttttga attaacgaag
    36901 aaggaccgcg acacagattt gtcacgatcc ttctttttta tacctacaag taaacagatg
    36961 cgtaaactaa tttatacgat cattctgcgt attgaagagt aaggttcaca ttttttggac
    37021 tctgattgtt ttcccctgga ttaactggta cggattctcc attcgctttg atcgttgcat
    37081 tgcttgctgc accaatcgtg atggtcgcat ttgttgctgt ttccggtaac acggtactct
    37141 gtgtttcatt tgctgccagt gtcccttgat aaacataggc attgtctact agcacaccta
    37201 accatacacg attgacagca gtaaattcaa gggttacagg cttggtagcg tctgtgatat
    37261 ttactgcgat ggcactgcca gaatcttctc ctgtagctat tgccattttc ttttcttcac
    37321 ttgattttgt tgttgattct actgtagaag actctttggt ctgcgtgctt tgctctgagg
    37381 aagaggactc ctcgactgat ccttctactt taagcgaaga agctgtgtct ccgattattg
    37441 gatcagcttg acgatcttgc caagtcatat agcccacgac aacgacaatc gccaaagcaa
    37501 ccaatcctaa taaaatgaca ggaagacttg tccaaaagcg acttggatgc gattcttcct
    37561 gatgcattgc ttttctagaa gtacttactg tttcaggctg cggacgttca gggaccgatc
    37621 ctgtcaattt gcttttccca tcaaaaacat cgactaaatg gtctccatct tctcctacag
    37681 cttcagcgta ttgccgaata aatgcccgca cgtaaaattt accaggcaat tggtcaaaat
    37741 catcattgtc gattgcttcc aaataacgtt tttgaatttt tgtaatctgc tgtaattcat
    37801 ctaaagatat ttttttattc aggcgtgctt gccgtaattt ttctccgatg gtttcaaaag
    37861 ccactcgttc atcttctcca gtctgtttga ttctccgaag catctgctgt tgcaacgaaa
    37921 gacgttatgt ttgggtgctg tgacaaaagt catgtcacag ctcctttttt ccgaataaac
    37981 attgttctcc tgccaactcc tcggtcacaa gtcacaatat ccaataaata taagaagctg
    38041 ttttttgaac ttgttccttg tgactcggag ttaaacggca ggctcacgac ctctttctta
    38101 aacggtgttc aatcagctgt tcctcggtat tagctcaaaa accggcaaaa tataaggaga
    38161 tatttttccg tatgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttcct
    38221 taaacggtgt tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacattaa
    38281 gaagctgttt ttacttggct gttcttatta cttgatgcag ggcagctttt tcacaacctc
    38341 ttggtacacg gtgttccaga agtagcttct tcggcaataa gccgaaatat cacaaaaatt
    38401 tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac
    38461 ctctttgatt acttcgtttg attttatttt aatataagtt gattaaaaga gagcatgatt
    38521 tgttttattt tctcactaat ttttccaaca caactaagaa taccataaaa gctgattttg
    38581 gaggaggttc ctactacaag tctaaaacga aatttaagac ttttcctcat aaaatactgg
    38641 tttattttgg tctcatgtaa aactcgctga aggcctcttc acttataaat gcttccccag
    38701 ctgccagtac gtctttcatt tggatcgaat cgatgatttc aggaagatca aataaagttc
    38761 gatcgccaaa taaactttgc gtaaattgat tggcaatata ttcgatcgaa tttaatgatt
    38821 ggaaatattg tccaagcatt ttcttcttca gcaaatcgag atttgtttct gacacttccg
    38881 gatcatcagc gaatccaaga atgatttctt tgactttttc tgctgctttc tctggttcgt
    38941 ccgtatctcc gctgaagtcc gcaaagtgaa attcacgatc caagctgaat tcaaaaccaa
    39001 agctgtcatc gataattcct tgattgtaca tcgctagata gtttcgagaa gtattgccta
    39061 aaatcatttg gaacaacaga ttgattgctg ttttatagcg taacagttct gttccttctt
    39121 gcggcaatgt gtctaatcct ttaataccaa gtacaaattt atctcgtgta atggctgctt
    39181 ccaatgcact ttgtttgatg atttcttttg tgttttcagg gaaataacgt acaatttctt
    39241 gctttggagg gaaatttttt gcttcttggt tttcccgaat aagtttcatc aatttctcag
    39301 gctccatctt accaactaca aataaaacca tattgcttgg ctgataaaac gtccgatagc
    39361 acgtatacaa gtcttgagct gtgattttgt cgatactttc aaccgtgccc gcaatatcga
    39421 tatgcaatgg gtgtgtagga tacaagttat ttagtatgcc aaaaaacatc cgccagttag
    39481 ggtcgtcttc atacatttgg atttcctgac cgatgattcc tttttccttg tttacagttt
    39541 cttctgtgaa gtaaggtgct tgtacaaaat cgatcagagt cgtcagattc ttttctacct
    39601 gatccgtcgt agaaaacaga tagctggttt tcgtaaaact tgtgaaagca ttggcggaag
    39661 ctccttgttt cccaaataat tgaaagacat caccatcttc tttttcaaac aacttatgtt
    39721 ctaaaaaatg ggcaattcca tctggaactt tcaccttttc tttttcacca tagggaataa
    39781 attcgttatc gatcgaacca taattcgtac tgaacaaacc atatgtcttg tgatagtcat
    39841 ttttaggcaa caaatacacg gtcaaaccgt ttggcagtac ttcatgataa agtgtttcat
    39901 tgatttgctc atattctgtt ttattcattt gcatttcctc ctgctaaaaa gaagattgcc
    39961 tgcaattcga tttgctcagc tacttgttgt atctctttga tcgttacgct ctccatacgt
    40021 ttcaaccatt cttcatcaga taattttgtt tccggcagcc agctgtccaa aaaagaagct
    40081 tcgattgccg cttgcggaga gtctagagaa agcagatatt ggtttctcag cattgctttt
    40141 gtctgagaca gttccaactc tgttatttca ccatttcgca aactttctaa ttgctcgtga
    40201 atcagatgaa gtacttgatt tcggttcttt tcatcgatac cggtttggac actcatgaat
    40261 cctcggaacg tatccacgct actagaggca taataagcta gactttcttt ctctctgaca
    40321 ttcatgaaaa gtttagagtg aggaaaaccg ccaaataaac cgttaaagac cattagcgca
    40381 aaacgttctg gttcatcgta atacacattt gtctgataag ctaggttcag ctttgcttga
    40441 acgattggct cacgtacttg tcgttccttc acgatattca cttgaggttg ggtataaaac
    40501 agatctggat aaactgtttc ccgcgactca aatggtaatt gcccaactgc ttcagctgct
    40561 ttgtccggat caacatcgcc gacaacaaaa atgtcgattt gatcatttgc catcatttct
    40621 tgatagtagg acgctagcga actagcagtg atcttgtcca aatcttcgac tacgcccaac
    40681 cctgggatct tttgttccgg gctgttttca aaataaagtt cttgcaaggc ataggaagca
    40741 aaagtttgtt tatcttcctt caaactttcc aaatatagac gcagattgtt cttttccaag
    40801 tcaaacgtca actgatcaaa ctgatgattt ttaatatttg gataaaataa aacctctttt
    40861 aaaaaatcta ccgcttgaga aaaaaggttt ggatcattga catattcacc attcacaaaa
    40921 ctgatccctg cgtttagcca atgtaaattt cctttttttc taactgataa tccaaagctt
    40981 gcgccataaa gctctgcaag ttgcgcactt aactttgtct gatccgaata attcaaacta
    41041 tttgtttcca acatgctgct gagaagtgaa cgtttcgtga tgacttcttg ttgcagacgc
    41101 gcagtaaaac ggataaaaat ccggatcgtt ttgtattgtt ttgttggtaa gacatgaaga
    41161 ttcacgcctt ctgccaattg atacaccata tttatatgct ccttttcaca gttgattgcc
    41221 cgcttactct tttaatgggt caacctgcct tttttactta ttcctctgct tgaaatttat
    41281 aagttcaggc aattcgccgt tttttctaaa tgatgttaca ctttatagta tagcatagat
    41341 gttttttatc aaaagaaaca gacggctaaa agcgtatatt gtttttttat tttttatttt
    41401 tcgatatact aaagacaaat aaaagaaggg agggtcattc atgatcaaag aattcaaaga
    41461 gttcatcatg cgtggagatg tcttggatct tgctgtcggc gttgttattg gtagtgcgtt
    41521 tacaagcatc gtaaaccaag tggtgcaagg cttgatcaca ccactaatag gttggtttgt
    41581 cgcaatgatc acaggtactt ctgatttaga aggagcactt tcaattcttg actggtcgcc
    41641 gacaaaaggc gtcacatttg cgtttggtaa cgttattagt gctatcatta catttctgat
    41701 cactggtttt gtcttattct tagttgtcaa agcggcaaac cgtgcgaaga atttacgctc
    41761 caaagaagag caagtggaag tggaagaaac tcctactgca gaagattatc ttagtgacat
    41821 acgcgatctt cttgccaaac aagtggcaga agacaataca catccagtag agcaaacaaa
    41881 tctttccgat acttctaaat aataaagaat ctaaaaaaaa gaaagccgat tttcagtgtc
    41941 ggctttcttt ttttagattc ttaacttgct acttgttcct ttttcatgtc gatatgtgga
    42001 atatcatctt ccaaatacac atctgagatt gccttgaatc caaacgactc ataaaaagct
    42061 tgtaggtatt cttgtgcgcc gatcacgatt ggtttttctg gaaaattttt ctggatcatg
    42121 ttaatcgttt cttgaacaat ctttcttcct aaaccatctt tacggttgtc ctgatgaaca
    42181 aggacacgtc caaaatggat actctccgct tcttcatata ctcgcgtgta ggcaaggatc
    42241 ttcccatcag tatcttgaaa aaacgtatgc caagcttctc catcaatgtc atcaacttct
    42301 tgataaggac actgttgttc cacaacaaaa actgcgattc gttctttgac gatcgcgtaa
    42361 aactctgctg ttgacaattc ttcaaatttc tttacctgat attccactaa gcaaacgccc
    42421 cctgcttttt tattcttagc ataccgattc gcacacaaaa aagcaaacga accttacgaa
    42481 tctcaggctc gtttgctttt taaactgctt cagttaaaaa ttcgatctca actgtccgag
    42541 taagtacatc tgaatagctg taagatactc gttcaaaaga attttcttca ggatccaaat
    42601 caacaacgaa tactgaagga taggtttctg ttaaaacgcc gcggcgttct gtttgacgct
    42661 ttcttcctgt ctgagcaacc aacatgattg ggctgccgat acgaccttct aaatctttct
    42721 tgattgaagc taaagttgtt ggcatacagt tcacctctac tatcaactat accacatatt
    42781 tttaaaattt tcaagtttac cataaaaaaa aaagaaatgc aataaaaata aataagaagc
    42841 tcattaagat ttttctaaat tattcctcat tataaaattt caagtgtttt tttattttga
    42901 gttttactct cccgtaagcg ctcattactt gcttgtgatc cattgctaaa cattcagcca
    42961 tgtctacaaa atcaacgcct tgcatataat aatgaaaaat tttagcttct atttttgaaa
    43021 actggatttc gcaattttcg attgcttctt ttaataaaag atgctgaatt gctaaaggtt
    43081 cttgcaatgt gatctctgct aaaaagtctg gtccttctgt ctctattttt tggtcgattg
    43141 agacttcatc tttttgatca cgacgcttca gcgcgttctg cttcctcaac agagaataga
    43201 tttgattgct tataacgctt ttataataac ttccaaagct aacacctttg cttttttcgt
    43261 aagttctcaa tgcctgatat aaagcgatcc ttgcttcttg ttcccaatcc tcagaaacat
    43321 aatttcttaa ataataggtg tttttcaaac gtagtaaaag tggaaaatat ttttgataca
    43381 gggcagcaaa tgcttcttcg tcaccattac gtgcagcgcg caataactcc tcagacataa
    43441 aataattcct ccataaattc gtttatggag gaatcttatc atcaatgatt catcgaactg
    43501 attagaacga tatgttattt attttttctc attaaatcgt ccaatttttg ggacaatttt
    43561 tttaactgct cttcattcca tggagaatta cgtcggaaat caataaaatg catatcttta
    43621 ttttcttggg ctattgtttt ttccgttttt tttatcgtct tatataattc ataagcagaa
    43681 gttcgtaatg ctccttggga aaagatcatc cactgttctg ctaaatcgct tgtcgccact
    43741 gtcacttgtg tcaaacggtc attcagctga ccagcgattc tctcgatata actgtcagcc
    43801 gtttcgtctt ctttcgtaaa aacaacatcc aactggtatt tcttataatt ttgttggatg
    43861 cccggaacta gttgggcatc gaagacaacg atgacttcca ttccttcata tttcgcataa
    43921 tttgataaac gatgtaaaag agcttctctt gcatccgcca ttttgtcctg ctttttaagc
    43981 tggacaagtt ccggccacga accaatcatg ttgtagccgt ctacaatcaa cagctgtttt
    44041 ttcatggtct actccccctg acgttgggaa aaagcctgat acataagcaa accagcagca
    44101 acactggcat ttaagctctg gacgtgcccg gtcatcggga tcgtcagcaa ttcatccatt
    44161 tcttttttca atcctggact cattcctttg ccttcattac caatgactaa agcaattgat
    44221 cctttggcat tccatttttg gaagtttgtg ccggtcatat ctgtgccaaa tacccaaaat
    44281 cctgtttctt tgagctgttg cacagcttgt accaaattcg tcacgcgtgc aacagggaca
    44341 tgttcgactg ccccagtgga agtctttgtt acgataggtg tgattccaac tgctcggtgt
    44401 tttggtatga tcacaccatc gacgccactt gcgtctgctg tccgtaaaat cgacccaaaa
    44461 ttgtgtggat cttcgatgct atccaaaatc aaaaagaagg ggtcttcttt tttctctttt
    44521 gttcgttcaa tcagttggtc aagggataaa tattcataag gtgtaatcgc aagaacgact
    44581 ccttgatgca cgccgtgatc gctcaaagtt tctaattttt gtttaggaac ccatttgact
    44641 ggcacagctt gttcacgtgc gatttctttc agttcttcta ctttttctcc tcgtgaatct
    44701 tcttgaagaa agagtttatt ccctcgccct tgttgtagcg cttctactgc ggcatggtgc
    44761 ccaaagacga aattatcttc taatgcttct tcattttgat cagaaactga ctgatttcgt
    44821 ccagtttgct ttttatttga atatctattt tgttttttgt cttcaaatcc acgtttcgat
    44881 tgatgtttgt ttgttctttc tttacccatt tttttcacca actttctgaa tacaccagcg
    44941 gatcaattct tccatccgct cggtttgttt tgtcaaatgc agatagccca tgagcgcttc
    45001 gaaccctgtt gcgacccgat acgtcgtaac atcagcattt tttgcgctcg tatggctttt
    45061 agcatttctt cctctgcgat aataaagttc ttcttcttct gtcaaaagat gttcttccag
    45121 catttgctga atcaaaaagg cttgtgcatt cgcagaaaca taattcgttg ccgcacgatg
    45181 cagcttattg ggttttgttt ctccttgatc aatcaggtgg tcacgaacat agatttcgta
    45241 aatcgcatct ccaacataag ccaaggctaa accgttcaat tgtgtgtaat ctctcatgct
    45301 tctcttctcc atcgggttcc ttgcggtgta tcttctaata agatcccttt ttctttcaac
    45361 aaatcacgaa tttcgtcgct tcggacaaaa tcacgattgc ttcttgcttg atttcgttct
    45421 tcgatcaatt gttcaatctc ttcatctaat aactcttctg ctgtaaataa aataccgaag
    45481 atcgaaagcc attgcgagaa taaagcttct ccttgtgcca agacagcggc agaaactttc
    45541 ggctggtcta gataagtgtt cagccactta gcaagctcat agaccactgt gatcccattt
    45601 gctgcattaa aatcatcgtc catttcttcg atgaatcgct gttctaaatt ttctaacaaa
    45661 gcgattttat ccgcatcatc tgccaaattc tctgaggcag tttcttggcg gaatttcaaa
    45721 ttgtcaaacg tattcttcag tcgttgtaaa ttggtggctg cttctttcat cgtcgtttca
    45781 ctgtagcgga tcggacgacg gtattgtgtg gtcgccatga agaaacgcaa gacttgtgga
    45841 tcaacgtttt tgatcatttc atgtacagtg atgaaattcc ctagagattt actcattttt
    45901 tcatcgtctt cgccaatcgt tacatagcca ttgtgcatcc agtaattagc gaattttttc
    45961 cctgttttcg cttcactttg tgcgatttca ttttcatgat gaggaaattc taaatcttgc
    46021 ccgccaccat ggatatcgat cgtttctcct aaatgttttg ttgccataac ggaacattca
    46081 atatgccaac cgggacgtcc tttgccccat ggtgaatccc aagaaatctc gccaggtttt
    46141 gcttgtttcc ataaagcgaa atctaatggg tcttcttttt gctgctgttc ttcccctgta
    46201 cgctgacttg ctccgacttc cagttcatcg atggattggt cacttaattt cccataattt
    46261 tcaaatttac gggtacggta atacacatca cctgaagatt cataagcata gcctttttca
    46321 accaacactt gaataaatgc taaaatatct ggcatatgat ccatgactct tggatgcagt
    46381 gtagcaggtt cgacattcaa cgctttcgta tcttcttcaa atgctcggat gaaacgatca
    46441 gcaacttctg gtgctgtaat acctaattcg tttgctgtac ggatgatttt atcgtctaca
    46501 tccgtgaaat tcgatacgaa atcgacttta tagcctcgat attcaaaata gcgtcgaatt
    46561 gtatcgaaag caatggtgct acgagcattt ccgatatgga tataattata cacagtcggt
    46621 ccgcagacat acataccgac atgtcccttt tgtataggga tgaattcttc tttttttctg
    46681 gtcaatgtat tgtagatttt tatcattctg atctcctcac tttttctcct ttgattcgaa
    46741 cgacttttgc tggtactcct accgctgtag cgtcgtctgg aacatctttc aacacaacgg
    46801 cacctgcacc gattttggcg ttttttccga tcgttaccgg tcctaatatc tgcgcattcg
    46861 cagagagcat ggctccttgt ttaacagttg ggtgtctttt acccgtttct tttcctgtgc
    46921 cgcctaatgt tacgccatgg aataaaatca catcgtcctc gatttctgcg gtttcgccaa
    46981 tgacgattcc cattccatgg tcgatgaaca cgccagttcc aatcgttgct cctggatgga
    47041 tctcaacacc agttaggaaa cgccagaatt gagcattgat cttcgccaat aaaaagagcc
    47101 ggtgctgata taaaaaatga gagaaacgat gccaaaacaa ggcatgaaaa ccagggtatg
    47161 tcaaaacgat ctcagctgtc gaacgtgcag ccggatcgtt tctctttgct gcatcaatcg
    47221 ctcgggtaaa ccaactcatg actttccact cctttcgtaa gaattttatg taggaaatca
    47281 tcttcttata aagaaaagaa gcgcctttta aataagtttc cttatcaaaa gacgctttcc
    47341 gcgtggttcc acttttcttc ataatcaatg attatctctg acagctaacg cctgttatac
    47401 gttcttggct actttgtttc ctataaaaca gtttttcctg ttttctgtgt ttcgccaaga
    47461 ccactcccag aggcatttcg gcaaccattc attgatacct tgcaccaccc ggtatctctc
    47521 ttgtaatgat cagctgctta cttttttctg ttcatcgtgt ttagttcagt acttgattta
    47581 aatgtgctaa tgatttttca cgaccaagca attcgatcgt ttcaggcaat tctggaccat
    47641 gcatttgtcc tgatacagct acacggattg gcatgaaaag attttttcct tttacgccag
    47701 tttctttttg aacagctttg atggcagctt tgacagttgg agcatctact acttccattt
    47761 gttctagctg tgctttgaat gcgtggagaa cagtaggaac tgtttcacca gcaagtactt
    47821 cttttgctgc ttcattcaat acaggatgtt cattgaaaaa tagcgtagac agatcaacga
    47881 tttctgcagc atagctcatt tgaggttgat acaggctaac gatcttcttc acccattcga
    47941 ttgtcttttc atctgggttt tctgctacac gtccatcagc gattagataa ggcagacaca
    48001 tgtcagtaaa tgcatctaga tccattgctt tgatgtaatg gttgtttacc cattgcagtt
    48061 ttttcgcatc gaaagctgcg ggtgatttac ttaggcgttt tggatcgaac attttgatca
    48121 attcttggtg ttcgaagatt tcgtcttcgc ccacaggtga ccagcccaat aatgcaataa
    48181 agttgaacat tgcatctggt agatagccta attcgcgata ttgttcgata aattgcaaga
    48241 ttgattcgtc acgtttgctt agttttttac ctgtttcact gttgatgatc aatgtcatat
    48301 gtccgaaggt tggtggtgtc cagccaaatg cttcatagat catcagttgt tttggtgtat
    48361 tggcaatatg gtcatctcca cgtaaaacgt gagtgatttt catcaaatga tcatcgaccg
    48421 caacagcaaa attataagtt ggcataccat cgcgtttttg gatgacaaag tcgccaccga
    48481 tattatcgga ttcgaacgaa atagcgcctt tcaccatatc tgtaaatgcg tattctgtat
    48541 tgcgaggcac acggaaacgt acaacagatt ctagaccttg tgcttctttt tctgcttgtt
    48601 cttcaggtgt caagttcgca catgttcctg cgtaatgagg catttctcct cttgcgcgtt
    48661 gtgcttcccg ttctgcttct aattcttctt ctgtgcaata gcatttgtat gctcgattgc
    48721 tagcaagcag ctgatcaatc agcggttgat agatttcttt acgttcagat tgacggtaag
    48781 ggccgtattc tccaggattt tcaggagatt catcccaatt cattcccaac caagcaaggt
    48841 tttccaactg gcttttttcg ccatcttcga tattacgttt ttggtcagta tcctcaatac
    48901 gaataataaa gtctccatca ttgtggcggg caaacaaata attgaataat gcagttcttg
    48961 catttccaat atgcaggtgc ccagttggac ttggtgcata gcgtacgcgt actttcgtca
    49021 ttttcttcct cttctttcaa actagacttt gactaacagc aaaaattccg tcagttacgg
    49081 cttctcaaat atcatttatt taaagccact cagcacaaat tttacctttt ctaaggtaaa
    49141 tagtaaagcc aactggttga atttccttac cagttggctg aatttctttt ttattttgat
    49201 tgtttagtct ttttctctgt ctctttttta ttgtcgattc ctcgtcctgc atgtgcaggt
    49261 ttagcaaaga tcatgcgtcc agcagctgtt tgaagggcac ttgttacaac gacttcgata
    49321 cgttcattca tgtaatgctg tccatcttct acgacaacca tcgtgccgtc atcaaggtag
    49381 gctactcctt gttggcgttc tgtcccggct tttactacta gaacattcat cgtttcacca
    49441 gggatgacga ctggttttac ggcatttgcc aacgcattga tattaagaac aggtacattt
    49501 tggaattcag agactttatt caagttatag tcattggtta caatgacacc atctaaaagc
    49561 ttagccaatt tgatcaattt gctgtctact tcactgatat cttcaaagtc tccatcgtac
    49621 atttcgacag atataccctc ttctttttgc aaggcattta ggatatctaa tccgcgacga
    49681 ccacgcacac gtttcaaact atcccctgag tccgcgatgt attgtagttc gtaaagaaca
    49741 aaattaggga tcatcaaggt tccttctaag aatccagtct ttgtaatatc ataaatccga
    49801 ccgtcaatga tcacgcttgt atccaagatt ttatatttat ggaaattttc tcctgatttt
    49861 cgttctaaca gttgactctc aacttgacta gctgtttctt gctcgatttt tttgcttcgt
    49921 gaaccaaaaa ctttcttcca ttcttcgatt cgtgtcgttc ccattctcaa tcccagatag
    49981 cctaagatga tcatcagtaa aatcggcaat acactattga cgaatggaat ttccatattg
    50041 taaagagggg tcgagatcac aacacctaaa gtcaatccga tgatcgcacc gatcgcaccg
    50101 aagagaagat aagtcaagct gacctcactt agtttttgtt ctacttgttt gataatagct
    50161 gagatatgtt tggccaacag caaagataat aaaaagaaaa taagtgcgcc taacaggctg
    50221 ttggtaaatg tattattcag ccatccgttt gactgttgac cgattgcctg ccaagctgag
    50281 ggtaaaaatg aaatacccaa gcttgcgccg acaatgatca tcagcaaagt aatgacacgt
    50341 ttttgcatcg tttttcctcc gtttcgacaa tcatttatta gttagcggaa cacacgcttc
    50401 aacgtttcag ccaaagttgc tactccaact atctcgattc cttttggcgc ttcccagtta
    50461 cctaagttgt ttttaggtag gtatactttt gtgaagccga gtttttggac ttctttgact
    50521 cgttgttcaa tatgactgac acgtctgatt tctccagtca gtccaatttc accgatgaaa
    50581 cattccgttg gctgtgttcc tttttcttta tagcttgaag caatactgac agcgatagct
    50641 aaatcaatcg ctggttcatt taattttacc ccacctgcag cttttaagta agcatcttga
    50701 ttctgaagaa gcaacccagc tcttttttcc agcactgcca tgatcagaga tacccgattg
    50761 aaatcaagac ctgttgttgt tcgtttggca tttccgaaca tagtaggtgt gactaatgct
    50821 tggatctcta ccaaaattgg acgtgtccct tccattgcca caacgatagc agagccagtt
    50881 gcaccgtcta ggcgttcttc taagaaaatc tgtgaaggat tagcgacttc ttccaatcca
    50941 tgctcatgca tttcaaaaat cccaatctca ttcgttgagc caaatcggtt ttttactgct
    51001 cgcaaaatcc gaaaactatg atgttgttca ccttcaaaat ataacaccgt atccaccata
    51061 tgttctaaca ttcttgggcc ggcgatcgag ccttctttgg taacatgacc gacgataaaa
    51121 atggctatcc catttgtttt agcggttttg agtagttctg cagtggtttc tcttacttgg
    51181 ctgacacttc ctgccacact tgtgatgtct ggctgcgtca tcgtttggat ggaatcaatg
    51241 ataacataat caggggagat atgttcgatg gcacgactga tttcgttcat atctgtttca
    51301 gcgtatagat aaaattctgt gttgatactt ccgaggcgtt ccgcgcgtaa ttttatctgc
    51361 tctgcgcttt cttcgccgga tacatataag acttttccgc ctatggctgc taattgctga
    51421 gaaacttgta acagcaaagt ggatttcccg atacctggat cgccaccaat caagaccata
    51481 gaccctggca ccacacctcc gcctaacaca cggttcagtt cttctaattt tgttttgatc
    51541 cgaggttctt ttttagggac gacgtctgcg atttttgttg gttttgcttt ttctcctgtc
    51601 aaacttgtcc gagttctgcg gtccgttgta tcttgttcga tttcttcgac catcgtattc
    51661 cattttccgc aatttgggca gcgtcccaag aacttaggcg acacataccc gcaattttgg
    51721 caaacaaatt gtacttttga tttttttgcc attgtaccgc ctccttttat cctaatcatg
    51781 agacattaaa tcaagcttaa gtatctttaa cgttttattt actggaagaa ccaaatccgc
    51841 cttgacgttg agcagtcgtt tgatcttgat ctgcttttaa aaacggcaaa aagatccctt
    51901 ggccgatacg ctcgcctttt ttgatcacta catccttgaa accaaagttg gtgaattgga
    51961 acatgatatg gccttcattt gcctcattat tgtaataatc actgtcaatc acacctactc
    52021 cgtttgtcaa cactaaaaac cgcttcaacg gattgcttga acggttagca agctgcaaaa
    52081 attcatcttc tcccatgtaa gctttgattc cagtaggcac gagggttggt ttcaattgtt
    52141 tttcattttc tgatacatct tttttcaatc catctgcgac tgtttccaac ccctttttcc
    52201 agcctgcttt ccagatactt ggaatcacga cctcttctgc tgcttcaaaa tcatagccag
    52261 cagcatgatg tgtagcgcgc tgcggtagat tgatattttg atctttatat ttagaaataa
    52321 cttcaaatcc tctttttctc accggttttc ctccctaaat aaactactcc tattctaaca
    52381 tatccgaggc tgtaaaagac tttctttaca aattcttcaa gaaattctgc tatcttaaag
    52441 taaaccatta attcgttctt tttccaaacg actgcaagtt gtacaaaaaa actatttttt
    52501 acatgacttt actcgtcaaa acaagtagaa tagaacgaaa ttaatgagtg gatatttgat
    52561 tgaggtgtca catgcgtaaa gaaattaaaa atgcaaaacg aattattgtc aaagtaggaa
    52621 caagtacatt gatttatccc aatggaaata tcaatcttgc ggcaatcgat cagttagctt
    52681 ttgtcctttc tgatcttaga aatcggggaa aggaagtgat cttagtctca tcaggagctg
    52741 taggcgttgg tatgcataaa ttacatttgt cagaacgtcc gactttgatt cctgaacagc
    52801 aagctgcggc ggctgttggt caagccgaac tgatgaatat ctataatcaa agattcagtg
    52861 tatataatca acagacggca cagatcctcg tgacaagaga cgtggtagat tatcctgaaa
    52921 gtcgtaaaaa tgtgaggaat acattggaac agcttttgat tatggacacg atccctatcg
    52981 tgaacgaaaa cgatactgta gcagtggatg aattagatca tctgacaaaa tttggcgata
    53041 atgatcagct gtcagccatc atggcaaaac ttgtcgcggc agaccttttg atcgtccttt
    53101 ctgatatcga cgggttttat tcagataatc cgactacaaa tcccgaggct cagatgtatc
    53161 gctatatcga taaaatcgat gaaaagattc tggcacaagc aggtggtgct ggtagcacat
    53221 ttggaacagg cggtatgcag agtaaattaa aagccgcttc taggatattt gaagcaaaca
    53281 gcgcaatgat tttagcaaat ggaaaaaatc cggcaattat ttttgatatt cttgcaggaa
    53341 aagaagtagg tactttattt aaggaggact tgaaatgact gacttgatgc aattaggaca
    53401 acaggcaaaa gatagcgcgc aacaactagc acttatgcct acaaaccgca aaaatgagct
    53461 acttgttcaa atggcacaaa ccatcaaaaa gaatcaacaa gcgatcattg aagcaaatca
    53521 taaagactta gaaaaagcag tcgaaaacaa tatcagtgaa acgatgcaag accgcttgca
    53581 gctgacacct gagcgaatca atgccatggc tacagagatc gaaaaaattg cttcgttaga
    53641 cgatcctatt ggaaaagtag atgagatgtg gacgaacacc gacggattga gaatcggcaa
    53701 aaagcgcgtg ccactaggcg tgatcggcat catttacgaa tctcgtccaa atgtcacgac
    53761 agatgcagct agtctagcat tcaaatcagg gaatgccgtt atccttcgcg gtggcaaaga
    53821 agcattcttc tcgaatcaat tattagtcca attattgcag caagtactgg tcagtgctgg
    53881 cgaatctcct tatgccatcc agtttgtcga tgatacttct catgaaactg caggacagtt
    53941 gatgcgattg acagaatact tagatgtgct gattcctcga ggcggagcaa aattaatcca
    54001 acgagtcaaa gaacaggcaa cggtacctat cattgaaaca ggtacaggaa ataatcacgt
    54061 atatatcgat aaagaagcac agttatctat ggcagtgaac atcatcgtca atgcaaaagc
    54121 ttcccgtcca tctgtctgta acgcggcaga aacattgctg atccattctg agatcgcacc
    54181 ttttttcttg ccagctattg aaaaagaact ggtagaacac ggggtctctt tacgtgcgga
    54241 tgctcgtgcg ttagaatatc tagagacggc tgctttagca gaagacacag attgggatac
    54301 tgaatatcta gattatatcc ttgctgtcaa agtggtcgat tctttggatg aagcaattgc
    54361 acatatcaat cggcataata ctaaacattc agaaacgatc gtgacagata gttatgcggc
    54421 tagtcaacgt ttcttaaatg aagtggacgc ggcggctgtc tatgtcaatg cctccactcg
    54481 ttttacagac ggatcggtat ttggttttgg tgcagagatt ggtatctcta ctcaaaagtt
    54541 gcatgcccgt ggtccaatgg ggttaaatga attaacttcc acaaaatata tcatttatgg
    54601 cgatggacaa atccgcgaat aatgtttcgt gtgaaacatt attcccgcct tttgcctttt
    54661 ttctgtaaag gttctatcca actagaaagg tgtcaatatg tttgaaattt taatgatttt
    54721 ttttatttac cttatttctc tgaatatcgc tgcttttctg ggagtcggta ttttatccct
    54781 tttctttcaa ttcaaaaaac gaagcatagg gagtcaacgg gaaaaatggt cgcagtattt
    54841 tgacaaaatc gggcaaaaag ggctggttac acgcttgcac attagttaca tggttgcatt
    54901 atgcttatta gcgacaatca attactattc ttttttcgat cattcaatcg cttatacgat
    54961 tacgctactg attgctggga tttttcactt atcttataaa tatcagctga ataaaaacca
    55021 cttgaatcgg actttccgct aaaaaatatc cgtacgataa gtagagtgcc ttgaagatcg
    55081 gcaaaaaact gccggacttt ttagaggctg tgacaaagat tttgtcacag cctctttttc
    55141 catataaacg gcgttcgcct accagctgcc tggtaaatct gtttcctctt cgttaagaag
    55201 cgaaaatccg tcaaatgttt cgccatcata ataacgaagc agctcatcca tggacaagtt
    55261 catcgttttt tctttttctt cttccgctga ttcagccagt agctgttccc gatacacctg
    55321 atacatacca gctatttttt gctgcatcgc ttttctagtt tcaccttccg cattgattat
    55381 tggcaattct ttgatatagc cacgataaat caaccgatca tctgaaaatt caatgggtaa
    55441 ttcaacgagt gtcaacccga cttcctgaaa atattcttcc agcatctatt atccccccat
    55501 tctttttctt aaatgaattt tagcttacct actgaaaatt ttccagtttc attattttga
    55561 gaaaaaaagg gaatgtgaca aaaatcgtgt cacatcccct ttttccgaat aaatggtgtt
    55621 cttctgccaa ctcctcaatc ataagtcaca atattcaaca aacatgagaa actgtttttt
    55681 gaaattgttc cttgtgattc agagttaaac gacagtctca caacctcttg gtaaacggcg
    55741 ttcaatcagc tgaccctcgg tattagctca aaaaacggta aaatatgaga agctattttt
    55801 ccgttttgtt tctctaatac ttaggataaa acgctgattt tacaacctct gttaaacggt
    55861 gttcaatcag ctgtccttcg gtattagctc aaaaaacggc aaaatatgag gaactatttt
    55921 tccgtttgtt tctctaatac tcaggacaaa acgctgattt tacaacctcc tgataaacgg
    55981 tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat gagaagctat
    56041 ttttgtttgg ctgttcttat gacttgatgc aggacagctt tttcacaacc tctgatttct
    56101 tattcttctt catttgtagc aaaataatta cggtgataaa tgatacaaca tacactgata
    56161 agaacaatgg cacctgcaaa ataaaacgca tagctttcac caatatgttc accaatcaca
    56221 cctgcaataa atggtgctaa agctccaccg aaaaagcgga caaaactata gctggacgaa
    56281 gcaacgtttc gttgcagacc tggtatttcc atagcaatcg tcgtcaacag tgtattgata
    56341 attccttgga aaaatcctgc taacacaatg ctgatcggta caaggactgg catagaagtt
    56401 ccagctccta ataacagcaa acaaataatg aataagacaa atgctgctaa aattgtccga
    56461 taagtcgtaa acttccgctc aagagccggt gcaacgaaaa tcgaagcaat agccagaagc
    56521 acaccccagc caaaaaatac aaaaccgact tctaattcag aatagccaat caataaaaat
    56581 ggcgcatacg caagtaaagt aaagaatccg aaattatata acaatgcgat tagtccaact
    56641 gaacgaagtt tatgattgct taacgctttg acaccttccc aaaggcctac ttttctcttt
    56701 ggtttctcag tttcaggtaa aagtaccgct agggctataa aaccgcaaaa catcaaaaat
    56761 gctaccccaa aaaatggata acgccatgaa ccactgccca agaccccacc gactagaggg
    56821 ccaacagcca ttccgcagcc aagtgctgct tcatacatca tgattgcttt ttctgtgtta
    56881 ccaactaaaa tactgacgat cgttgctaaa gcagtagaga caaataaagc attcccgaca
    56941 ccccagccgg cacgtaaccc aatcagcatt ccgatattcc cagataatcc gccaagacca
    57001 gcgaatacaa cgatgataaa caatcctgtc atcaatgttt ttttcaaacc gatccgacta
    57061 gataagaaac ctgtaaatag catcaccaca ccagtgacta acatgtaact agtaaataac
    57121 agggtcgttt cagctggcgt agcgtttaac tgtgcagcaa tcgtttttaa gattgggtct
    57181 actagaccca ctcccataaa agcgatcata caggtagctg ctacaactag cgctgaactt
    57241 ttcccctttg tttcttcatt tgtttgttct tgtatatcta tttgattctc caatatttcc
    57301 atccctttct ctctattagc atcatacatg atttatttta catgtcaata tgcacatgta
    57361 aaacgtggaa actttactgt gatataataa agaaaataag acgagaggta ggatttttat
    57421 gaatacttta gcctatatat tattatgcgt gcttttatcc aaaccacgtt ctgggtatga
    57481 attgaagcag ctgatcacaa ttttttggga agcccatcac agtcagatct atacaacgct
    57541 tgccaagtta gaccagcagg gttatgtgac cgttttggac aaagacgagc actctcaaaa
    57601 aaagatctac cacttgactg atgaaggacg tgctcttgta gaggaatgga tcaaagagga
    57661 aactcccgct cctacgcaaa aagatgaatt tctagcaaaa atctatgctt tttcgacgct
    57721 cgataaaaca acagctactg gactgctatt cactagagaa caacagctga acaaaatcat
    57781 gcaaaaaaat caaaaaaaat tgaatgacct ctcgctagac aagaaacaag aatttggacg
    57841 ttatgttgtg atccagcgca gaattatgct ttgtcagcag gaactccaat ggtgtcagca
    57901 agtaagagaa caaatgcagc agttttttga gtaagggata aacagaacct ttctagaaat
    57961 atctattgaa gagcaaatta tcttcaataa aagagcaata cagttggaaa agaaaaagcg
    58021 atccgaacga caggccaaaa cctatcgttc ggatcactat ttttctagaa ctgcattcaa
    58081 gagactatga caaaaagcat gccaaggcct tttttcaaat aacagtgttc catcattcat
    58141 tactttgaat tgcttttagc acacttcttg aaagcgggcc aacgatgatc aactgcaacg
    58201 gtaaagcggc aatcacattc attttccaag cattcatata agcagagaat aattgatcag
    58261 gtattccttg ttccatgatc acaccgaata aagacataca tgtcaccatt cctaaaacca
    58321 tcaagcatga aatagttaag atcatttgga ccttgctttc tggatttact ggtagtttaa
    58381 aggcaatttt tttcgccagc acaccgatga tgaagacatc caaaatgaaa gccaccacga
    58441 atccaggcac tagaccaatc aacaaacttg ataaatgaag atcttgatgc agccataaat
    58501 tatacgcgct catccccaag accatcaaaa aacacatcat tgttgtaaaa attattcctt
    58561 cttttttatt tgtaggcaat ctgtaccact cctttaaatt tgtcacgaaa atcatcgtag
    58621 cacatttaaa aatttctcgt aagtactttt tttattttct gcccttttat ttcataaact
    58681 tcatcagccg tacgctcgac aaagtaagaa tcatgagaag taaacaaaat cgttcccgga
    58741 tatatttgca ttagcttctc taaagcttcg atcgttgcca tatcaataaa attagtcggt
    58801 tcatctaaca gcaacacatt acttggctta gtaaaaagca aagcaatcgt cagacgggtc
    58861 gcttctcccc cacttaatgt acataacggt ttcgtcaaag tttcctgagc aaaccctaag
    58921 tgattcagca aactacggac aagcgattct gaaaactctg tttgtctcat gacaaaacga
    58981 atgatggatt cttcagatgt catttgataa cccttttgct gatatacttg aaagcttact
    59041 ttcggagaga gaaggattcc ttctccttgt ttgcggatat gttctaataa ggttgtctta
    59101 cctgaaccat tttcaccgac aatcgcgatt tttttaccta aagaaaactg aaaatcacca
    59161 tttaccagta atgttctccc acttctttcc aattggacat tttgtcccat gatgggatag
    59221 cggctgtgga gttcaagaga ttttggtact ggaaaacgga tttgtttcat ctgctgcggc
    59281 ttggttgttt ctcctatttt ttggagtctt ctctctaatg ttttcgcatt cttttggatg
    59341 gctttttgta cactgtcttt ttgtttagag gaagacaacc gatccagtct gatttgttgc
    59401 tttttctttt tactggacac tttttccaac ttttccgctt gttttttctt ttcttggatc
    59461 gatttttcca atcgtttttt ctctttcatg atcgactctg cttctcgtag ctgagtcttt
    59521 ttctccaatt ccttttgagt gagataggca ctataattcc ccgaaaactc tcggatttct
    59581 ccgtctttta cttcccagat tttcgatgct agctcatcta gaaaatagcg atcatgacta
    59641 acaaaaacca gcgtaccata ataataacga agctcttcaa tcagtcgatc aatacttttt
    59701 ctatctagat gggttgttgg ctcgtctaga agcatccctt gttcataaga agaaagaaga
    59761 cgtatcaact cgatctttcg ttcttcccct ccgctccatt tccgctctga agtttcttcc
    59821 agtcttaact ggaaatgact ggctaattcc aaatttaggt cctttgcttc agcgagatag
    59881 gttaattgag gcaggtagtt gaaggtgatc tctgtttgga ttttcccatg atcgggaaaa
    59941 agttcaccgg ctattagttt gagcaaagtc gatttaccga caccattttc tcctatgatc
    60001 cctatacgat cttgctgata gacacgaaga gaatcaatag atagttctag ctggttgcca
    60061 aagctgactt gtagatttgt tatatttact gctaaatttt ccatatgttt tacactccta
    60121 tttcatgtaa aacaacaatt atcgatacta aaaaagagca gaaaaatctg tttgcaaaaa
    60181 aactgcatca catcttttcc tactctgaac gaaataacta tgactaaatc accaaatctg
    60241 tcatagcttt cacctattct actgtcgttc gaaaaaatgg acatactcca tccatgaaat
    60301 tgttgtttat acatgtcttt tgaatttatt tagttgttat tcaaaagttc gaaacgtaat
    60361 ttcatcgatg cagtcatttt tccgacctcc ttttttatta ggttaccaca cacgattcaa
    60421 aaaaagcaat agctactgaa tagtaactga aaacctttta ttcgaacata gtgatctttt
    60481 ctggtgctaa acgaacagac tcgtaacctt cttctaccgc ttttcctatt gataaaataa
    60541 taactggaac ataacggtct ttatccaaac caaatgcctc tgccaactga tctgcttcga
    60601 atccaccaat tggatttgtt tcatagccat gatcacgcgc aactaacatg aattgcatag
    60661 ccgctaagct agaatcgact ttgactacat cgttcatttc ttctcttgaa aaactcttgt
    60721 aataaggaat gatcgcagct aattgttgat ctctgacttc ttttggcatc ttccctgatt
    60781 cgtaagcttg gttgtagatt tcttcgccat attcgtaaca ttgcatatcg ccaaaaatca
    60841 tgaccattgc agaagaagta tcattttgtc tcgtattgaa acggatcaaa ggacgcaaga
    60901 tttcttttgc ttcctcgctt tcagcgacca caaagcgcca tggtttcata ttgacagaag
    60961 aaggtgcagt cgtcgcttct ttgatcattt ccagcatttc ttcgtgtggg attttgtatg
    61021 tttcatcata tacgcgaacg gatcttctac cagtcataat tgttgaaaaa tcattttttt
    61081 gttccattat tattaacctc tttcgtttct gttgttatat tgcaaagttg ggtattcaat
    61141 ttatctaaaa gtgttagaaa ttgtttcatt tctacatcgc tgaaagccgg ggatagcacg
    61201 gaacctgtat ctttatggtc catttcacaa tgcttgattt tcttctgggc tttttctgtt
    61261 agcgctacat acacttcccg gttgttctga ggatttctct gacgcgaaac atagccattt
    61321 gcttccaata ccttcagatg acgagtaact gctccttgat caatctgcaa cttttcttgc
    61381 aaactgcttt gtagcaatgg attttcttct ttcagtctca tcatgatctc gtatctagta
    61441 agacttatac cgatattttc ttcaaagatc tggacgattt tttgatcggc taatttgatt
    61501 tgatataaaa ggtgactaat ctcttctaaa gacataattt cctcctttct aataattgac
    61561 tcgtcaacga ttgacttgtc aataatagaa taaaaaaaga aaaaagtcaa agaatcagct
    61621 gtgtaataaa aaaaacgctc tcaatagaaa agtatagaga aaagaaattt tttgtagaat
    61681 gaacggaaaa aatgataaag aaagcacaac gctaaaacca agattattga ttcttatcaa
    61741 actatagaag atagctcttc tgcagtagat aattttacac cgccaaaaat ttccgaaatc
    61801 gccttttgca agaatcccgt cgaatattag aaattggatc agtatgctta aacagtaata
    61861 aaaaagttat gacaagatgt tgtcacaacc ttttttgagt aaacggtgtt caaaaacttg
    61921 catctgcggt aataagttca accaaacaaa aaacatgaaa atctgttttt gtttggctgt
    61981 tcttactact tgatgcaggg cagctttttc actacctctt tttaataacc ataaaatcct
    62041 attccttcat atctccagcc catcggtgtt agccaatctc tttccttagc actcaaggta
    62101 tagtgatggg taccagtttt ttcatttgga ttaaatagtc gataaattgg cttggaacca
    62161 ccagaataaa aacttagacc ttcataacgc caaccgattt ttgttaaaga gttgatctca
    62221 gattgtgcca ttgtgtaatg atggtctcca ctgtaaggat tgtataaacg gtagacaggt
    62281 tgtccgctgt ttggtgcttg ccaagcaatc ccttcatatt cccagcctac agttggcaga
    62341 taattttttt ctccggcatt caatgtatat aaatgttctc ctgtatgacg gttatacaga
    62401 cgatacatcg gagaagttga agttggtgct gtatcatact gtgtcagatt ataggtttcg
    62461 attacattgt tcaatgtaga accataatta ggcgcagtgg cataacgacc tgttagccaa
    62521 gccgtagcat ctttgtaaga gttcgtattg cttttccatg caccggaata gtagtaaacg
    62581 cctggctgaa aagaagtcgt cttcaatacc gttacgttat cttgcaatga ttctttgtac
    62641 gaaggatatt ttctaaaagc ggcattcacc gtaatccatt gcccattcac atattccgaa
    62701 gtaggcatcg taacagattg gccattatag cttcctttaa tcccaaatag attgtaatta
    62761 ggggcagaag ccaaagtact gttcccccag ccactttcaa cgatggcttg agcaatcatg
    62821 actgatgcat ataagtcatt ggcacttgcc aaactttgtg cagttgaggc aatactatca
    62881 atgaacgctt gttgagaatt agcagatgag cgagcataaa aagcatagcc agtatagttt
    62941 tcgaattcag acaaatctgc ttgatgggta tgtgtcaatg tttgcccatc tgtcaattgg
    63001 ttcgtgattt cttctgcatt ctctggattg ttctgagctt ctgctgatgt actgtcttcg
    63061 gtttgtgttt gttctgttgt ttcagttgtt ggttcttcgg ttgaaattgt attttctgag
    63121 acagtagctg actgcaaagt actttctgta tcgttttcaa ttcctgtttc tgaagaactc
    63181 gatgtctgag ttgcgacagt ttctgtcgat tcttcagcta gcactgttgt gttggcaact
    63241 gctagatttc ctaaaagaat aacgctaaat aataaattct ttgctttcat cttttttctc
    63301 ctttgaatta tgaaactttt cctaaataac agtgtaacta aaaaagtaaa agcaagtcaa
    63361 tgtccatatt taaataacat ttgtttcttt ttaataaatt tgctttttct gcataaaaag
    63421 acgtaaatga agaatattct tctccattca cgccttaaat tgatggtcgt ttgaataact
    63481 atttaacaag taattctctc tggatattat ccgttatgga ttatacttcc tgtgtcgtcg
    63541 gacggataat catgttgctg acagacatcc ggtctggcgt ttctatcgca aatgctacag
    63601 cagaagctat atcttcagat gttaatcccc attctttttg cgcttcatga agttcaagag
    63661 cagccttttt gtcgctgatg gtttgatata attcagtttg aacagctcct ggagagatga
    63721 tcgtagattt gatgttattt tgccgttgct cttgacgtaa tccttccatg attgcacgga
    63781 cagcaaattt tgttccgcaa taaacggcgg aatcaggata aacaacatgt cccgccacag
    63841 aatcagttga aatgatatgc ccagattttt gtttttccat gattggaagt acggcggaaa
    63901 ttccatttaa cacacccatg atgttgatat caagcatatt ttgccattca tcacggtgac
    63961 cttcaactaa tggagcggtt ggcatgattc ctgcattatt gtacaataca tcgattcgac
    64021 cgaacttttc catcgctaaa tcgataactg cttggacttg tgcaaaatct cttacatctg
    64081 ctgtttgaat atatagctca gcttctggta aagattcttt tattgctttt aggcgatctt
    64141 ctcggcgtgc agctataact agtttagccc ctttttctgc taatagcttc gttgttgctt
    64201 caccgattcc gcttgaagcg cccatgatga caatcacttt ttctgataaa tcattcattc
    64261 tattctcctc ctgttttgaa aaatctcttg cttatactca ctatctctta tttcatctta
    64321 ctgttcaaat aatccgttcg acaggtttga cgattttggc agggattcct gcaacgatgg
    64381 tattgtctgg gacatctttc gtaacagttg cattcgctgc aacaatggca ttctcgccaa
    64441 tcgttacgcc tggcagaata gttgctcctg ctccgatcca agcatttttc ttgatgtgga
    64501 ttggctttac tgacaagcct cttcgttctt ttggatcggt tagatggttc accgtgatga
    64561 ggttgacacg aggaccaatc aatactgaat cttctaaaca gatgccacct aagtcaacga
    64621 acatcacttg acgattgata aagacatttt tccctatact aatatgtttg ccgaaatctg
    64681 tataaaaagg cagcagaaca gtcaccgtat catctacttt gtcactaatg atattttcta
    64741 gccgtttgcg gatatctgct ggttcatggt acccagtatt cagcgctgct aaaggctctt
    64801 cgttttccgc ttggatctga tggatcactt caaagagcgg atccgttact cgaatctctt
    64861 tattgacgat ttgtttttcg atgaggcttg tcattttgct ccctcctttt atccttactt
    64921 tataacggac ggcttatgat gtctaatact tataaaacat acttaactat tccttttaac
    64981 tatacttagt ggtatgcttt tttaaaaaca ggagggaaaa aatggatatt cgtatactga
    65041 cgtatttttt agcagtagct agagaaaaaa atatcagccg agcagcaaaa tctctgcata
    65101 tcacgcaacc tactctctca aaacaactga aagatttaga agaagaactt ggtgtgacac
    65161 ttttcactcg aggctcccga gagatacagt tgacggattc tggtcgatat ctttattcaa
    65221 aaggaaaaga aatcttgtcg ttggtagaaa aaacggcgaa caatttaaca aatgacgcaa
    65281 tcatctctgg agaaatctac attggcggag gcgaaactaa agcaatctct ttcatttctg
    65341 atagccttca ttcgttgatc aaggcgcatc ctgatatcca gtttcattta tatagcggga
    65401 atgcagatga tatcatagac aaactggata gcggattact tgattttgga ctcgtaatcg
    65461 aaccgacaaa caaacaaaaa taccagtcat ttcgactgcc agcccaagat cgttggggac
    65521 ttttgataca tcggaatcat cctttagcaa agcaaacagt catccatccg caggaactga
    65581 aaactgttcc tttgatcatt tcccggcaaa cgtcagtgga cagtcaacta gctgaatggc
    65641 taggtttcag catagatcag ttatcgatcg tcggaacata taacttattg tacaatgctt
    65701 ctttgatggt cgataacggg acttttggtg cattagctct agatgggatc atcaatactt
    65761 atgggacgga aaaagtattc atcccactcg atccgccatt acaagcttca ctgaatctca
    65821 tttggaaaaa agaccagcct ctttctcctg ctgctcgtaa atttttagat gtcctaacgg
    65881 agaaatacag ttaagtagag tttttttcct tcgctttcag ctttttaaca acgaataagt
    65941 taagcaaaag atccaatgat taaataacat aagtttctta atttgttcct tccttttatt
    66001 tctgataatt ccagtaaatt actataatta taaaaaaaga attgacaaaa cataactgtc
    66061 tatataaaat tgattacgtt aacaataatt cgtttcatgt gactagatca tactaggcat
    66121 gaaaaatctc tgaggaacat ggtttcccta ggaggttttt cgtgtctatt tttgtttggc
    66181 cgcaatcaac tggtctagca aacgtgaaaa ccaaatcttc aaaaagggtg cgcgcatgaa
    66241 aatcaaaaag atctttaatc aaaatgcagt attggtagat gacggtggac aggaaaaagt
    66301 agctattgga aaagggattg gatttgacag aaaaagaaat gatttgattt ttgatcgtga
    66361 tgtcgaacgg atatttgtca tggaaccaga aggtcaaatc aagttgcaaa atttacttaa
    66421 tcagattgat gaacgctttt tatttgcagc agaaacgatc attgatcatg cggaaactgt
    66481 tttgatggaa aagttgaatg aacatgtatt gatcgctttg accgaccaca ttgctttttc
    66541 tgcggagaac attaataacg gtatcgtcat ccgcaacaaa cttttaagag aaatcgaagt
    66601 gctttacagt gatgaattca gtattgctca atgggcagta gagtatctga caaaagagct
    66661 tgggattcca tatacttatg acgaagcagg atatatcgca atccatattc acagcggacg
    66721 aagcggtcgg aacaataacc atcgcagtat ccgtgaagtg acgattgtat ctgacatcat
    66781 ccatttagtg gaatcagaat tggaaacaga tatccattca gaaccatttt cactgaatta
    66841 ttcaagactg gtgaatcatt tacgactgct tctacaacga acccatgcgc aacaatatgc
    66901 tgttttagac acggaaatcg tagaaatggt caaacgaaaa tacccagaaa gctataagat
    66961 ttctaaaaaa atccgtgtgt tgctgaccaa agaataccag ctcgcgatca cgaaagaaga
    67021 actgggttac ttggccatcc atatcgagcg tttaagaagc gcaatcgtac aaacaaatgt
    67081 taacaatcat gaggaggaaa aaaattgaaa gcatatctac aacgaatggg acgttccctt
    67141 caacttccag ttgcggtctt acctgctgct gctttactag tagggatcgg aaactggtgg
    67201 gcatcttata gtaacgacat tgtcgctcat tttttacaag ctggcggtaa cgcagtttta
    67261 ggtcaattac ctttgctgtt tgccgttggg ttagcactag ggatgtcagt ggacaaatca
    67321 ggtgctgctg ccttggcagg tttggtcgca tttgaagttc cagtaaatgt tttaaaaact
    67381 gattcagtag ctactttatt gaatatcaaa ccagaatcag ttgatccatc ttttgctcag
    67441 atcagtaacg tatttattgg tattattgcc ggattgattg cggcagctct ttataatcgt
    67501 ttccacgaag caaaattacc gatggcttta tctttcttta gcggcaagcg tctagtgcca
    67561 atcttggctg cctttgtcat gttgctgatc agtgcagtgc tactgctcgt atggcctccg
    67621 gtttactctg ctttagtttc ttttgctaaa ttcattttga gtttaggctg gggtggtgca
    67681 ggtctttatg gcttcttcaa ccgtttactg atccctacag gattgcatca agcaatgaac
    67741 tccgtgttct ggtttgacgt agcaggaatc aatgatattg gtaaattttt ggcttctgaa
    67801 ggaacaaaag gcgtaacagg aatgtatcaa gctggatttt tcccaatcat gatgttcggg
    67861 cttccagcag gagcatatgc tatttacaga aatgcacgtc cagaaaacaa agcaaaaaca
    67921 gcttccttga tgttagcagc agcttttgct tcattcttta ctggtgtaac agaaccactt
    67981 gaattctcat ttatgttcgt tgcttggcca ttatatgtct tgcatgcaat tttcactggg
    68041 atttctttag cagtgagtgc attcttccat tggacagcag gatttgcctt tagtgccgga
    68101 tttgtcgatt acttcctatc tttgaaaaat ccgattgcga atcagccatt gatgttgatc
    68161 gtccaaggat tagtaacagc agtgatttac tactttggct ttaactttgc tatcaaaaaa
    68221 ttccatctga tgactcctgg ccgcgaggaa gcagatcttt cagatgacga tactgcaacg
    68281 actaattcag acaataaata tgcggcacaa gcaagcaaaa tctacgctgc attaggcgga
    68341 gccgacaatg taacttcgat cgataactgt actacacgcc ttcgtttgca agtcaaagat
    68401 acaggcctga tcgatcaaaa taaaatcaaa gccactggcg tacctggtat gaagatcatc
    68461 gacggaaaaa atgcgcaagt catcgtaggt acagaagtac aattcgttgc agatgagatg
    68521 gcaaaactac atggtggtgc tgcaacacga cctgcacaaa caaacaccgt ggttaaaaca
    68581 gaaacatttg ccggtggaac gatcgaacaa gatatttacg ctatcgcaaa tgggaaactg
    68641 attccaatca ctgaagtttc agatgatgta ttcgcggaaa aaatgatggg tgacggctat
    68701 gctgtattac cagaaaatgg agaaatcttc tctccaatcg ctggtacgat cacaaacatt
    68761 ttcccaacga aacatgctgt cggaatccaa acagatgctg gtatcgaagt cttgcttcat
    68821 atgggcatca acactgttga cttaaaaggc gaaccattca ctttatatgt aaaagaaggt
    68881 caaaaagtcg caagaggtca attgattgct ttagttgatc ttgccgctat tcaatctgca
    68941 ggcaaaaaca cggatatggt cgttgtattc acaaatggcg acaaagtcca aagcttagaa
    69001 atcgaacccg cacgtgatgt aaaagcaaat gataaaatcg gctctgtttc caacaaagcc
    69061 tgagccatcg tcttccccag acttttttat cgataaacaa acaggagctg agacaaaaat
    69121 cttgtctcag ctcctttttc cgaataaaaa cggcgttctc ctgccaactc cgcggtaata
    69181 agtccagcca aacgtaacat atgaagagct attttttatt tgactcttct tattacttga
    69241 tgcagaacag cttttttaca acctctgata aacggtgttc aaaaaccagt ttatcggtat
    69301 gaagtcaaat ttatgcaaaa atgtggaaag ccattttcac aaaatttttc ttcatactca
    69361 aaactaatcg gctttttcac aacctcttta tacattattc ctgatccaat atttctttta
    69421 atgctgcttc aagagaagtt aatggttttc ccaatacttt ttctaaatca tcagaggaaa
    69481 tagctaactg atccttttta atatcatgtt ggatcgctaa aaacatgtca gctgcttctt
    69541 gagggaatcc ggcttctttt agattttcga caaacgctgc atcacttgct tcaataattt
    69601 ctaacttttt acctgtcgca cgctctaatg ccttggctaa ttcttcatag tcagttagat
    69661 tgccggataa ttctaagatt tctggagagt cactttctac tagtactttt gctgcagctt
    69721 ctgcatattc acgtttcaat gcccaacctg tttttccatt tcctgcagca taaacgaatt
    69781 gaccagtttt taaagcctgc ccaatgatag gtaattcatt ctctacatac caattattcc
    69841 gtaaaaatgt atgggaaata cctgaatcct taatcatttt ttcagtgaat tggtgatctg
    69901 gcgctaatac actgatcgag tgatcggcat ctgcaaaact tgtataagcg atataagata
    69961 ccccagctgc ttgtgctgcc ttcactacat tcttatgttc ggcttgacga tttcctggaa
    70021 cgccagagac aaaaagcaat cgatctattc ctttcaacgc ttctttcatt gagtctaaat
    70081 catcataatc accaatacgt atattaaatc cagcttcttt cagtgcttct cctttttctt
    70141 cactacgtac tagtacatag atttcatttt gtggaaccat cttttttaag aattccagcg
    70201 cataaccacc aaaacctcca gttgctcctg ttactaaata atgcatattt gccactccct
    70261 attcttttta tttgttaaga tttacagctg tacatttata aattacacct ttatcataaa
    70321 gagtgtggta agataagtca agcaaaaaaa atgaaaggat gacaactgat gaagtattct
    70381 ctgcaattta gtgatgcaat ccatgtactt gcctatattg aaatgtttca agatacccga
    70441 tcactttcta gtgagatgat tgccagtagt atcgaaacaa atgcagcaaa tgtacgtaaa
    70501 atcatgagtc agctcaaaaa atccggtttg atctttacaa aaaccggaaa accgcagcct
    70561 tcccttgcca aaagtccaga tcagatttct ttactagatg tctatcgcag catcgaaggt
    70621 aatacacagc ttatccaagt cgatccaaaa acaaatccaa attgcgtcgt tggtgcgaat
    70681 atccaagaag ctttagaaga aagctacgct cggctgcaga aaaaggcaga agaagaaatg
    70741 gctaagatca cacttgatag tctgatacat aaaatagccg ttttggaaaa agaaaaaaga
    70801 ccagaaaaca caggcctcgt agagagattt ttataattct ctctactgtg cctgcatttt
    70861 tctgatcgga atcgtctaca taaattcttt tattctccgg gggacaaaag ccttgtcatg
    70921 cccctttttc ctctctaatg ctcaggtcaa agcgctgaag tcatgacgtc ttgataaacg
    70981 gtgttcaagt agctgaccct cggcattagt tctgaaaaat gcaaaatatg gaaagctatt
    71041 tcccattttt cctctctaat gctcaggtca aagcgctact ttcacgacct ctatattatc
    71101 atggtttatt gactaccggt atccagattt ctcccatata tttcccgttg gtaactccca
    71161 tttcgactac agcatttgga cccccaacat aggcgtaatc ttctacttct cttaaaacat
    71221 gcccaaaggc cgtattcgtc aaatcatttc ttaattcttc aggcgattct gctaaagcga
    71281 ttacaacaag atacgttcct tttgggaatg ggatgattcg ggtagtgttt ggaacatcct
    71341 gactcgtttg aacaccgaca tagtacatca ttttcccgtt atagggttcg ttgactgtaa
    71401 agaaataatg attttcagca gcttgtttca atgcatctat tgttccatta ccgataactt
    71461 tttgctcaaa tgcttgtttc tctgcagcca aaccgacaaa atccgtattg ctactcttca
    71521 attccacacc aatacccgtc accgtaaaat ctggtttttc ttcgatccta tactttgcca
    71581 tttctcccac cctttctatt atattaagtt catcttacat aaaattaaag ggaaatgaca
    71641 cgattacgct cttttttcta gggatatttt tattttttaa ctattttttt gctttcactg
    71701 caatccaaat ctcactgtaa acattactga aatcctctcc ccattttgta aaagagatct
    71761 cgggagcttc gactaactca taatctgaag aaggaagcca ttctgcatag attttccccc
    71821 aagtatcttg taaagtttgg ggaaatggcc ctttattagg aaaaatggcc caagtgtgtt
    71881 cttctatagc aagcatttct aaatcagcgt acggattttc ttttgtcgta gcaaagccaa
    71941 tcatgtgcat caaactgccc ttttcttcca agcgggctcc atcaaatcca taagaaacgt
    72001 tgattacttg atttggatat aaatctccta acttatgcat ctcttccctt tgagtctccg
    72061 tgatcgtctg cgctaacttt tgtatttctt gattgactcc ttcaaatttg acaggaactt
    72121 ttttcgccac acctgccaca taaaactgtt ctttcttttc gatacgaaat tccatcgatt
    72181 ttcctcctct gacatcaata aaaaatgtca agcgtggaaa agatttttgt atgccgtatc
    72241 tgctgatttc tgaaggcaga tatcctgacc aatcacgaaa agctcttgaa aaaccttcga
    72301 ctgtttcata tccatatcta aacgccgttt ctgtcactct tgatccatct aggagatcgc
    72361 gattagccaa agccaatcta cggtttttga tatagtccat tagcgacatc cctgcaataa
    72421 acgtaaagat gcgttttaat tgatattcag agattcctgt attttcggct attattttat
    72481 aggaaagtcc ttctgtcaaa tgttcttcga tgtaatcgat caattggttc agatctttaa
    72541 gcatatttat cttcctcctt gcttgccttt ttatcatatt atcaaagaaa gtcaattact
    72601 tgatgaatca ggaaccaatt tagtcagatg tagaaaatgt tgtatggatc taaagaaaat
    72661 tctcaagcag cgtttcgtaa cggataattg gtattatctg cctatctatg ttttaccttt
    72721 agaaaaataa attttgggga agacacattc taagtaaaca aagcggtaaa aatatctttt
    72781 attcccttag ttaatccgct tactacctac tgtacaaagc aaattgttgt tcaaactgct
    72841 gatcagaacg ctgcttggat ttttctttgc gatacatcat acctctttta tcaattggaa
    72901 caattattta tttctaacaa aatcacttca tttttgtcag ataaataaaa aacaaaaaaa
    72961 gttccatctt taaaacacgc tattctttta ctttttctca cattctctaa gaaaagaaaa
    73021 ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc aaatatgtaa aatcagctac
    73081 ttttttctta aacgtttctt tatactaaat ataaataagt gtttcatgaa aggagaacta
    73141 gatgaataat tggcttgagt attttcctga aaacgttttg gaacgcggtt acagctatca
    73201 tctgcacggt tttgtccgtc atttgaacta tacaagcaag tacttgtccg caacagtttc
    73261 tggcacggaa gattataagg tagtgatcac ttgggatgaa aagaccaaca tgacttgtga
    73321 ttgtctatat gccatagaag gaaaaaaatg caaacacatg gctgctgtct tattcgctta
    73381 cgaagaacgt ccaataaaaa aatccaacta ctcactttcc gaactctcgt cccttgtttc
    73441 ctccgctagt tcctctcttg tacgggagct tttaacagag attcttatag aacatccgca
    73501 atttatcgaa cgcttcaaag ttaagatgcc atttcatgca ataaattatt cagataaatt
    73561 aacaactatc atccataaat acgatcatat tatcaagaaa aacaaaaaca ggaaaacagc
    73621 gaagttcatc atggagatgc gaaagttcat ccaagaagca gttgaatcgc tcattcagca
    73681 aaatgcctat cttcccgcat tcgaattgat caatgaagtg attgctactc tcgaaacatt
    73741 ctattgggag cctgaggatg aacgaaccct tcttctgata gaggactgtt attatttatg
    73801 gaaagaatta ttagcagaag cacctcatgc agaaaaaagg caaatgttct cttggttcgt
    73861 ctgccaagtc gatcatactg acgcctctta ttcaaaacgc tactccataa aaatcctgaa
    73921 agaagacttt cgggaaaaag aattttctaa ccaaaagaaa aaaatagata aaaaaactaa
    73981 agaaacggta aagaaagacg atttcaatga aaaatagcaa gtaaacgata acaaccatcc
    74041 taaatccaca agaaatacag cagaccttgc tttaaaaaca cgaatcatgc aaaggatgaa
    74101 gtaaagaaga cttaacgcgt acccaatatt agtcatttga ctattaggta cgcgttttgt
    74161 tttataagtc atgacaaatt tccttcgttg ctgggtagat tttccggcat tgttgataga
    74221 ttttatgata cttttctaca ttttgtaaga gaggttccac tgcatctgta tattggataa
    74281 aattctgaca acaactttca ggtgtgtcga accactctaa tctttaaaga taaatattaa
    74341 a
//
LOCUS       DACCYH010000004        66901 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.004, whole genome shotgun sequence.
ACCESSION   DACCYH010000004 DACCYH010000000
VERSION     DACCYH010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 66901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.004"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(26..328)
                     /locus_tag="IVY85_000241"
     CDS             complement(26..328)
                     /locus_tag="IVY85_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8043285.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="IVY85_000242"
     CDS             complement(353..2446)
                     /locus_tag="IVY85_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8043286.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="IVY85_000243"
     CDS             complement(2935..3699)
                     /locus_tag="IVY85_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP8043287.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IVY85_000244"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IVY85_000244"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP8043288.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="IVY85_000245"
     CDS             complement(4704..5780)
                     /locus_tag="IVY85_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP8043289.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="IVY85_000246"
     CDS             complement(5804..6397)
                     /locus_tag="IVY85_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP8043290.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IVY85_000247"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IVY85_000247"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP8043291.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="IVY85_000248"
     CDS             complement(8067..8366)
                     /locus_tag="IVY85_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP8043292.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10119)
                     /gene="mdcA"
                     /locus_tag="IVY85_000249"
                     /pseudo
     CDS             complement(8468..10119)
                     /gene="mdcA"
                     /locus_tag="IVY85_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            complement(10126..11037)
                     /locus_tag="IVY85_000250"
     CDS             complement(10126..11037)
                     /locus_tag="IVY85_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8043293.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11157..12014
                     /locus_tag="IVY85_000251"
     CDS             11157..12014
                     /locus_tag="IVY85_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8043294.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12201..12563)
                     /locus_tag="IVY85_000252"
     CDS             complement(12201..12563)
                     /locus_tag="IVY85_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043295.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            12909..14348
                     /locus_tag="IVY85_000253"
     CDS             12909..14348
                     /locus_tag="IVY85_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8043296.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14355..16907)
                     /locus_tag="IVY85_000254"
     CDS             complement(14355..16907)
                     /locus_tag="IVY85_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP8043297.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17021..18352)
                     /locus_tag="IVY85_000255"
     CDS             complement(17021..18352)
                     /locus_tag="IVY85_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8043298.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18318..19796)
                     /locus_tag="IVY85_000256"
     CDS             complement(18318..19796)
                     /locus_tag="IVY85_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8043299.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(20101..21936)
                     /gene="tdc"
                     /locus_tag="IVY85_000257"
     CDS             complement(20101..21936)
                     /gene="tdc"
                     /locus_tag="IVY85_000257"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8043300.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            22406..23833
                     /locus_tag="IVY85_000258"
     CDS             22406..23833
                     /locus_tag="IVY85_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8043301.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(23925..24290)
                     /locus_tag="IVY85_000259"
     CDS             complement(23925..24290)
                     /locus_tag="IVY85_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8043302.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(24287..24652)
                     /locus_tag="IVY85_000260"
     CDS             complement(24287..24652)
                     /locus_tag="IVY85_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8043303.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(24844..25092)
                     /locus_tag="IVY85_000261"
     CDS             complement(24844..25092)
                     /locus_tag="IVY85_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043304.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            25091..25219
                     /locus_tag="IVY85_000262"
     CDS             25091..25219
                     /locus_tag="IVY85_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP8043305.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            25276..26970
                     /locus_tag="IVY85_000263"
     CDS             25276..26970
                     /locus_tag="IVY85_000263"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP8043306.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            27070..27243
                     /locus_tag="IVY85_000264"
     CDS             27070..27243
                     /locus_tag="IVY85_000264"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043307.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            27621..28754
                     /locus_tag="IVY85_000265"
     CDS             27621..28754
                     /locus_tag="IVY85_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP8043308.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            28785..29105
                     /locus_tag="IVY85_000266"
     CDS             28785..29105
                     /locus_tag="IVY85_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043309.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(29134..29598)
                     /locus_tag="IVY85_000267"
     CDS             complement(29134..29598)
                     /locus_tag="IVY85_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8043310.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(29730..30983)
                     /locus_tag="IVY85_000268"
     CDS             complement(29730..30983)
                     /locus_tag="IVY85_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8043311.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(31012..31329)
                     /locus_tag="IVY85_000269"
     CDS             complement(31012..31329)
                     /locus_tag="IVY85_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8043312.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(31355..31675)
                     /locus_tag="IVY85_000270"
     CDS             complement(31355..31675)
                     /locus_tag="IVY85_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8043313.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(32139..33554)
                     /locus_tag="IVY85_000271"
     CDS             complement(32139..33554)
                     /locus_tag="IVY85_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8043314.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(33571..34887)
                     /locus_tag="IVY85_000272"
     CDS             complement(33571..34887)
                     /locus_tag="IVY85_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8043315.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(35037..35735)
                     /locus_tag="IVY85_000273"
     CDS             complement(35037..35735)
                     /locus_tag="IVY85_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP8043316.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(36024..37013)
                     /locus_tag="IVY85_000274"
     CDS             complement(36024..37013)
                     /locus_tag="IVY85_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP8043317.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(37093..37926)
                     /gene="msrA"
                     /locus_tag="IVY85_000275"
     CDS             complement(37093..37926)
                     /gene="msrA"
                     /locus_tag="IVY85_000275"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8043318.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(38101..39735)
                     /locus_tag="IVY85_000276"
     CDS             complement(38101..39735)
                     /locus_tag="IVY85_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043319.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(39722..41569)
                     /locus_tag="IVY85_000277"
     CDS             complement(39722..41569)
                     /locus_tag="IVY85_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043320.1"
                     /translation="MFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKDNSSILKI
                     ELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRIKGLSS
                     IFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNICTILI
                     SFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAYGLI
                     FNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVVNLV
                     KAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITTYTM
                     GLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQFPWR
                     ILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQSKHG
                     HIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAADNNI
                     TYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEITTKY
                     TPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            41556..43193
                     /locus_tag="IVY85_000278"
     CDS             41556..43193
                     /locus_tag="IVY85_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8043321.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(43338..44078)
                     /gene="truA"
                     /locus_tag="IVY85_000279"
     CDS             complement(43338..44078)
                     /gene="truA"
                     /locus_tag="IVY85_000279"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8043322.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(44269..44904)
                     /locus_tag="IVY85_000280"
     CDS             complement(44269..44904)
                     /locus_tag="IVY85_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP8043323.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(45051..46292)
                     /gene="sstT"
                     /locus_tag="IVY85_000281"
     CDS             complement(45051..46292)
                     /gene="sstT"
                     /locus_tag="IVY85_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP8043324.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            46490..47488
                     /locus_tag="IVY85_000282"
     CDS             46490..47488
                     /locus_tag="IVY85_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043325.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            47501..49519
                     /locus_tag="IVY85_000283"
     CDS             47501..49519
                     /locus_tag="IVY85_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP8043326.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(49579..50295)
                     /locus_tag="IVY85_000284"
     CDS             complement(49579..50295)
                     /locus_tag="IVY85_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP8043327.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(50522..51064)
                     /locus_tag="IVY85_000285"
     CDS             complement(50522..51064)
                     /locus_tag="IVY85_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8043328.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(51069..51296)
                     /locus_tag="IVY85_000286"
     CDS             complement(51069..51296)
                     /locus_tag="IVY85_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP8043329.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(51375..53255)
                     /locus_tag="IVY85_000287"
     CDS             complement(51375..53255)
                     /locus_tag="IVY85_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8043330.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(53444..54058)
                     /locus_tag="IVY85_000288"
     CDS             complement(53444..54058)
                     /locus_tag="IVY85_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8043331.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            54200..54760
                     /locus_tag="IVY85_000289"
     CDS             54200..54760
                     /locus_tag="IVY85_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8043332.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(55065..55379)
                     /locus_tag="IVY85_000290"
                     /pseudo
     CDS             complement(55065..55379)
                     /locus_tag="IVY85_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(55633..57268)
                     /locus_tag="IVY85_000291"
                     /pseudo
     CDS             complement(55633..57268)
                     /locus_tag="IVY85_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(57707..58576)
                     /locus_tag="IVY85_000292"
     CDS             complement(57707..58576)
                     /locus_tag="IVY85_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8043333.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(58596..59717)
                     /locus_tag="IVY85_000293"
     CDS             complement(58596..59717)
                     /locus_tag="IVY85_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP8043334.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(59719..60114)
                     /locus_tag="IVY85_000294"
     CDS             complement(59719..60114)
                     /locus_tag="IVY85_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8043335.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(60133..60930)
                     /locus_tag="IVY85_000295"
     CDS             complement(60133..60930)
                     /locus_tag="IVY85_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8043336.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(60932..61720)
                     /locus_tag="IVY85_000296"
     CDS             complement(60932..61720)
                     /locus_tag="IVY85_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8043337.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(61734..62210)
                     /locus_tag="IVY85_000297"
     CDS             complement(61734..62210)
                     /locus_tag="IVY85_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8043338.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(62282..64939)
                     /locus_tag="IVY85_000298"
     CDS             complement(62282..64939)
                     /locus_tag="IVY85_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8043339.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKR
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(64958..65362)
                     /locus_tag="IVY85_000299"
     CDS             complement(64958..65362)
                     /locus_tag="IVY85_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HAP8043340.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(65594..65851)
                     /locus_tag="IVY85_000300"
     CDS             complement(65594..65851)
                     /locus_tag="IVY85_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAP8043341.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(65976..66308)
                     /locus_tag="IVY85_000301"
     CDS             complement(65976..66308)
                     /locus_tag="IVY85_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043342.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT        20640 a        13456 c        10828 g        21977 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tgcattggct aagcctgctt gtttttgaac
     8821 agctagtgca tctaattctt cgacaaagac tggtttttta tttgaaccat atgtttcaac
     8881 catttgaaca actaattttt ggcctttccc taatggatca tcattgtcgc gaagtgattg
     8941 ccatgctggt gtagaatgac gacgaccgcc aggattatgt cccatgttag gcgcaccgcc
     9001 aaaaccagat aaacgtcctt ttgttacggt agaagagttt ccttcgtaat ctaattgcaa
     9061 ggttgaaccg ataaacatat cgatggcata ttgtcctgca gcttgtgata atgttcggtt
     9121 actgcgcatc gtgccgtctt taccgacaaa aaagacatct gggcgagcag caatatattt
     9181 ttccatacca acttcgccac caaaactatg gatgctttca acccaacctg attcgatggc
     9241 tggaatcatc gttggatgtg ggttcaatac ccagttcttc gcgattttcc cttttaaccc
     9301 tagtgattca ccataagttg gcagtaacaa ttcgatagct gctgtattga atccaatacc
     9361 gtgattcaaa gattgtacgc cgtgttctgc gtagattcct ttgattgcca tcattgccat
     9421 tagaatttgc aattctgtga tgttttgagg atctcgtgtg aataaagctt ctaattgata
     9481 aggtttatct gctttcacga caacgtctac ccagtcacca gggatatcta cacgtggtaa
     9541 agaatcgaca acttcgttaa cttgaacgat cacgatacca tctttgaaag ctgcagcttc
     9601 gatgattgtt ggtgtttctt ccgtattgaa acctgtgtac aagttgccgt ctttatctgc
     9661 tttatctgca gctactaaaa caacatttgg aatcaaatcg atgaataagc gtccataaag
     9721 ttccaaataa gtatggatat cacctaattt cattgtgccg tcttcaatca tttgggcgac
     9781 acgcaagctt tgtgcaccag cataagagaa atcgatcttg cttgcgattc ctttttcaaa
     9841 aatattcaaa tgttctggtc gtgagatact agacatgatc aaatgcagat cattcacttt
     9901 ttctgcatct actttttcaa gactttctga aagaaaactt gcttgttttt gattgtcgcc
     9961 ttctaataca actttgtcgc cgggagcaat cagtttttcc attacttcta cgattttttc
    10021 agtttctaca tacttgccat tgagcaagtt gctgattttt tccaatcgct gcgctttttt
    10081 ttcgcgttta gaagcccagt tgcggtttgg tttttccata attagtcatc tccatttttg
    10141 atatagaaac gtggcgtcat caataccatg ttggtctaga ttgatcgtac ggatcccttt
    10201 atctacacca aattttgtat gaatcagctt tgtcaaagta ttgccggggg gaaattcaat
    10261 cgtcagatcc attcctaatt cttttgccaa gtccatcatt tggttccact gcacagggaa
    10321 agctaggttt tccaacaaat ctttacgaat ctcttctgcg tcctgagtgg ctcttccatc
    10381 cgtatttttt atatacaaac atctagcatc agatagatgg acagactgca tatacggctt
    10441 gaatttcttg accgtatcat tcatcaagag acagtgagat ggcacaggga ctttcagcag
    10501 cttcgctgtt tgcgcttggt gccgtttggc ttctgccaac gtaacggata aaccttcgat
    10561 actcccagat aacgtatgtt gcatcggaca attttcattt gacagataaa ctggatggtc
    10621 aggatcaaat gtttttgcta caatttcctc agcttcgctt ctcgtcacac ctactactac
    10681 gcccattgca taaccgcttg gataagcttc tttcattaag actgcgcggt gatagactag
    10741 ttccgctgca ttctcaaatc tcaaagatcg acaagcaaca gctgcacaaa aagcaccaat
    10801 cgaatgtcct gctgcaatat caggctgaat accgtttttt tccatttcat ctaaataaaa
    10861 tactgctttt gtcagtaatg ctagctggat aaaaaccgta tcttgatagt tttcttcttt
    10921 atcaactaac cgataccccg tcacattttc taaacgttcc atgtacattt cagggatatc
    10981 cgctaacatc cctaacttct gactgccttg tccaggaaat aagaaacctg tactcatgag
    11041 cctcgcctcc tcagcactta ctttttccac tgctatctta gtcaatttct gctataataa
    11101 aaaatgccaa aaaaagaaaa aaccataaca aaaaggtatg gatgggatgg ataattatga
    11161 aattgcttca tctgcagtat tttaaaaaag tattcgaaac aaaaaatgtt acccaagcag
    11221 ccaaagaatt atttatttct caacctgcct tgagccgtgc gatcaaacat ttggaaaacg
    11281 aattaggtgt ccctttgttt taccatagcg gcagaaacat tgagacgacc gtttatgcag
    11341 aagaatttta tccatatgca gtcaagacat tagacacatt agaagaagga atcaaattat
    11401 tgtcttcggt caatgagaag atcgttactt ccgtcatatt gtatttagag gtagcctctg
    11461 tttctattcc gaacttggtc cgaattttta tggaaaagca tcctgatatc caattaacga
    11521 tcatgcaaca tcatttacca gatgatatta ccgatcctgt actgtacatc acttcggaga
    11581 tgaagcctgg catgaccaat attcctatca tgaaagaacc tgtttatgtc gccattccta
    11641 agaaacatcc tcttgctaaa aaagaacatc tgcaattaga agatatccaa cgcaccccca
    11701 tcttgatgct gtcaaagagc aacgccttcc gtcatacact ggatgcagca cttgaagcga
    11761 aaagtatcga attgacgatc agttcgacga cagatgatcc tgcgacatta cgttctattg
    11821 tcaagcaagg attagctgtt agctttttcc caaaagtttc ttggtcttac gataaaaatg
    11881 atcctttcgt cttgcgggaa atcaaagatt tgcccttgac acgaacaatt tatttatctt
    11941 ctagcttcac agaagatcat cctttgatca aaatgattgc aaaaacgatg cgggaatttt
    12001 atttaggaaa ataaaaaaaa aagagctggg acaaaattct tgtcgcagct cttttttcga
    12061 acaaacggtg ttccagaagt aatttcttag aaaataagcc gaaatttccc aaaaatttga
    12121 gaagcaattt tcgtaaattc cttcttattt cttgaagcta aacatttctg tcacaacctc
    12181 ttctctattg tcctagacat tcattgcgtt gatttaaaac ttccatcaat caaatctgct
    12241 agttcatcta gcttttctat tttacgtaga tcaatagaga tccaatgttc tttattcatg
    12301 tgataggctg gaaaataccc ctcttttcct cgcaaaattc cgccaagttc aggatcgacc
    12361 ttcaaatcaa gaatattgat gatcctatgg tcacttgcat catcatagat tttttcttct
    12421 ggaacagaca tcaggacccc aaaccatttt ttgctttttt gatgtctgaa tacgcaataa
    12481 tcaggaaatt ttttaaaaat atattctgga gacaaattgt atgtttcatt gatgtgatcg
    12541 attagttctt ttttcgttag catctgcttc catctctttc ttattgattc gctttattca
    12601 aagataccac gtaagaatag aaaaatgaat acaagtagtt gctaatcaaa tacttcgata
    12661 tttttctttt ttatcaactc ttaaatgttc aatccgcctg tgttggtctt gctaccatct
    12721 actattaaaa tgtttattat tgtttgccca cctatttttt gattttagtg gtatcatttt
    12781 tcaagttcat gatgaaattc ttatcatcag taaatgctcc agaaaaataa acaatgattg
    12841 aaaaatcagt tgaaacttga ccaccgttta ttttctccgt attcttagaa tagaagggag
    12901 tctgattgat gtattcacta atgaaaaaaa tcattaccga aaaagatatt cgacgtcatg
    12961 tttctttagt agaacaattg ctgaatcata caaagataac cgtaaatgaa ttagctgaaa
    13021 tcatagggac aaccgaacga acgatttttt ctgaccttca atctattcgc tctcagctgc
    13081 ccgaaggctg ggacatcatc agcgatcaag caggaatcag tttgcaaaac cagcaaaatt
    13141 tattaacaaa tgacctttgg gagatttttt tcaaacaatc agtcagtgtc gaactgctga
    13201 aaaatctgct ttttacaaaa aaagttgctg tccctgattt tctagccgac catgggcttt
    13261 cttatgggac attgaaaaga catgtgacta aaatcaatca gcgtttagca agttacgact
    13321 tgcagatcga tttgacgaaa tatacagctt gtattcttgg aaaagagcgt gcgattcgta
    13381 ctttttatca tcgattgctg attcctttca ctcataacaa ctattttttc gaggattatt
    13441 cgatccatga atcccattat tttcaatttt taagaaacct cagtcaaact gaactagcgg
    13501 ttgaaacaga agagattttc ggaacatgtt ggttctttat caatacgatc cgtataaaag
    13561 cgaattgccg attagattct tctatccata ccaacagcac actctcctcc ctgtatgact
    13621 ctgcattgaa aaaattgtat ttaacggaag gtatttatct taaagacact gagctttctt
    13681 ttgcttcttt ctgctttctg gaaagttgga attacaacaa taactatgga caagaaaccg
    13741 ctcgctgcct gcatcattct ccatttttag aggtcctcga aacgtttgta gaggaactag
    13801 cttcagagct gtctcttgat caactaaaaa aaacatctct gactgataac ttggctctcc
    13861 tcatattaaa atatcatgaa tcgccgatat tgattgaagc attggaccgt caataccatc
    13921 gttttttaga aacatatgat cagcaatatg cacacttgta tcaacagaaa gagaatttac
    13981 tgaacgatct aaaaaaagaa atagaaatca atgaaccaaa ttactttctc caactcttgt
    14041 ctctactgat ccaacaaacg attttttcta tcaaaccgaa tttgttcaat gtctatttta
    14101 ttttccaagg agaaccttca tggaaggcgt tcttgcagca ggaattaaaa gattacttag
    14161 gaaaacgtgt ctgcttctta cctatcgaac taaccgatct ttccactatt tcttttgaaa
    14221 aaacagatat cttgatttcc aatttccctc tagattctct agatatccct attgtataca
    14281 tttcttctat tccaacgaaa aatgagctca atcgtttgac tgaactcact ttcaaaactt
    14341 ttttatagta acggctagcg actgtattct ctagctgttt tttctttttt gcgaaacagt
    14401 ttgttcatga tccctttttc tatttctaca actaagaata gaagaatcac atttgctcca
    14461 atcaataact gttgtgtcat cgttaaactt actgtaccga tcagctgctg gccgactggt
    14521 agaaaaataa ccatcccttg caataaagca aggataccta atgaaagaaa aagcgctttg
    14581 ttttgcaaaa gaccttggtt caatgacggc ttggacaact ctctgcaatt gatcatgtaa
    14641 acagcttgag ctaagacgat attttgcaga agaatggttt gctgtaagct ttgcccttca
    14701 aactgcatag ccaataaata ggcaggaacc atgatcagta gggagacata gatgatccga
    14761 aacaaatgat aaccagatag gattccttgc tttggatctc ttggtgggcg cttcatgatt
    14821 tctggatctg gcgactcgaa ccctagtgca taagataaag tgattgtcgt taccatattg
    14881 acccataaaa tctgtactgg ggacaaaggt agcgggcgat tcagcataag tgcccaaatc
    14941 aagatcaatc cttgagctaa tgcagttggc aagaagaaat tgatggtttt ttgcaaatta
    15001 tccaaaatcc gccggccctc ttttacggct tttgcaatcg tatggaaatt atcatctcct
    15061 aaaaccatat cagctgcctg tttgcttact tcactacctt tgattcccat agcaattcct
    15121 acatcggatc ttttcaaagc aggtgcatca tttacgccgt ctccggtcat tccgacgatt
    15181 tctccatttt tctgcaaagc tttaacaatt cgcagtttat gttctggtgt cgttcgagca
    15241 aagacatcga ctattggcac ttgctgaatc aactcttcat cagacataag atccaaatcg
    15301 atcccttcta aaactttggc agtatgtttt aagccaattt gttccgcaat cgcttttgct
    15361 gtgtctttgt gatcccctgt aatcattttc actgagatcc ctgcttgttg gcattccttt
    15421 acagctttga ctgcactttc ttttggcgga tcgatcaatc cggcgatccc tgcaaaagtt
    15481 aattgggtta aagtctcatg cgtgagttcc tgtttcgaat ctactttttt ataagcaaat
    15541 cctaaaacac gctgaccttt tctcgcaaat tctgcagctg tctgactcca tgcttcttgt
    15601 tctgcagaag aaagagttga tttagcaaat aatacttctg gtgcaccttt gacgtaaatc
    15661 accgctcctt tttcctccgc atgcatcgtt gccatatatt tgtattcaga actgaacgga
    15721 atctttgctt ctattttttt caaaggtaga ttggctgttt ctgtgtgtcg aatcagcgca
    15781 acttctgtcg gattgccact taaattttct gttttctgtt cttcttttgt ttttaattcc
    15841 tgacagtttt tcataatgga caacacgcag gcttcttctt ttgcagctac atccacaact
    15901 gtcatttcat tttttgtcaa tgttcctgtc ttatctgaac agatcactgt catagaacct
    15961 aatgtttcta ccgaaggaag tcctttgatg atcgcctttt cttttgccat ttcatgtact
    16021 ccaacggata gaatcatggt caaaacagct ggcaatcctt cgggtaccat tgacaccacc
    16081 aaagcaatcg tagccgaaag aaggatatgc caatccatac catatcgaaa tgtcgtaaaa
    16141 ataactaaga ataggctcaa acaaagaatg ccttgaaaaa tttgtttgtt taatcggttg
    16201 atttttttaa tcagaggcgt cgtttgttgt tcgaccgatt gcaatgcttg attgatttta
    16261 ccgatttctg tcgtgtttcc tgtttctaca acgacaccga ctgcagaacc agattgtaca
    16321 agcgttcctg aaaaacccat atttttctgg tcgccgatcg attcttcttc tgtcaaaacg
    16381 gcatgacttt tttcagatgc ttctgattct cccgtcaaaa tcgattcttc cactaataaa
    16441 tcatgtgctt cgattaatcg gatatctgct ggaacgacat ctccggcacg taagacaatg
    16501 acatctcctt ttaccagttc tttcgcaggg attttttctt gcatcccgtt tgatagtact
    16561 atcgcttctt gtcccatcag ctgcttcaaa ccattcaatg attcttccgc ttttctttct
    16621 tgccagtaac taacgagtcc attgacgatc actacgaata aaataatact tccttcaata
    16681 tattcactag aagcaaattt caaaaaagag gcaatgatca acacgatcat cagcagatca
    16741 gtaaaatgct ttgctatttt tttccatcgt tttgtctgct gccttgcttt tatttcatta
    16801 tatccttgat cagtcaggcg tttctgtcgt tcttcttgcg acaagccttc tgttgatgtt
    16861 tgatagtttt tcaatacatc ttctaccgtc aatctgtacc agttcatcct atcccctcct
    16921 gtttaattga tactgttatt atacgcaggg aagttttttc aggcgtatcg attatttcag
    16981 gggggtctga aataattcgt tttgcaaaat gtatctgggc ctaactgata tttctattgc
    17041 ttattctttt atatacaaaa tttaagaata caaaccagat aaccaataaa aaaggaataa
    17101 agcgtgtcgt gctttcgaat aacaagctga aagcaattga agtaaaaaac accaacaaag
    17161 caagatcttt atatctttgt gtagtgttcc gtttcttttc tttaataaat tgaacatgtg
    17221 cacacaagat cgaagaccac acaaatagaa aacaactcgt tgtcatgcct gccaaaagct
    17281 gaaaaaacaa tcgactatca gctacaaaaa aatgtagaca aagaccggca aaaaataaca
    17341 ttcctgtaat taaaataccg tgtaaaggta cgcctttctt agaaagcgca caaaaccaat
    17401 ggggcaaact tttttctttt gctgtttgat ataaaatcct gctagtactg tagactgcac
    17461 tatttgctga ggacaaagaa gcaaccaaaa tcattagatt gacaattgct gctccgcctt
    17521 ggcaatggat cacttgcagc atagtcacta aaggactttc ttttaaatct aaacaatgcc
    17581 atggaacaac aaataaaatc gacaaaatcg ttcctatata gaaaaagaga atacgccaag
    17641 gcagacagtt gattgctttt ttcaaattct tttgcggatc acttgtctcg ccaacggtta
    17701 agccgatcat ttcaattcca gcaaaactga ataaaaccat aggaaatgag actaccattc
    17761 cagcaaagcc aaacggtagt aattcatcga tctgtccaaa attttctttg attccgatag
    17821 actgggtatg aaaatctgag acactcaatc ctatacccaa taagataaaa aggaaaatcc
    17881 ccaagatctt gatgattgag aaaaaagatt ccaattcacc aaaccacttg actgcaacaa
    17941 ggttgactgc cattaagatc attagtgcgc tacctccgct tatcaaagct ggtacatgta
    18001 caaaccagaa atggatatac aatcctattg ctgttaattc cgcaagtcca gctccaagcc
    18061 agcaaagcca ataagtccaa ttgacaaaaa atgcccactt tttgcctaaa taatagtcaa
    18121 cgaaatcact gatagagtta aatgtactat ttgctaaaaa taaatcaccc atggcccgca
    18181 tcaaaccata aatagccaaa ccgcatatca aataaacgag aataacagct ggccctgctg
    18241 atgcaattgc ctttgctgaa cctaaaaata atcctgttcc aattgcgcca cctaatgcga
    18301 ttaactggat atttcgatta gttagagtcc gtttcattgc cggtttttcc atcaagttca
    18361 cctttttctt tttcaagcag catggcgact tcctgttccc aagaaggttt ctttattttt
    18421 tcaatacaga gtggtactac aaacaaaatg acactgatgg ctgtgactgc aattagattg
    18481 atccatgttc cagctttcat cgtcagtccg acagaaaatc cgattgccgc aaataaaaca
    18541 ataacgacag tccaaactcc aagattgctc tttttctctt tttttccaat tctaaatggt
    18601 cgattcaact ttgcatgtgt ataacgcaat ctcaaaactg cgatccccat gatgacatag
    18661 acgatacagt agataacagt agttgaattt acgagcatca aaaatgcttg attgacgcca
    18721 ggaatcagca aataaaaaag tgcaaagatt gagataatga cagactgagt caaaataacg
    18781 gatttggata cgtcaaattg attcgtcttc caaaaattga attttggtgg atacgcacct
    18841 tttctagcac tttctgtcac tgtctttgct ggtcctgagg cccaagcaga taattggact
    18901 gctacaccaa tgaagacaag taaactaaac agattcacaa taagatgtgg caaaccaaga
    18961 atttggcaat aaatcgaaat agattgcgcg atattattca attccatttg tccttttggt
    19021 acgacatttg ctacaactaa agcattcaaa atattgacga aaaatataaa aattaacgcg
    19081 gcaaatactc cgcgaatata cgttttaact ggattttcca atcgagtgat atacacagag
    19141 gacatttcga taccagtaaa aataaacatg atcggcgcaa aataaacaaa tgaggatgca
    19201 gtcgtagtat ctggtattaa tttgtcccat gaaaaactgc ctaatgttcc ggttggtacg
    19261 atcccaactt tgatccacgc agcaaaccct aataagagca tcatcgtcaa aggcacgtac
    19321 aatcctaacc aaacaccgac ttttccgccg atttttgcca tatcaaattt caaattcaaa
    19381 aaagtgatga tccaataaac gactaaaatg acaactaatg taaatttgct gttcaaacca
    19441 agcggcacat tatcgataat attaccaaac aacggagcga gtgctgaagc gaccatcacc
    19501 atcccaggaa acatttggac ccataacagc catgagacaa cgaaccccca tttttttcct
    19561 agagcattcg atacccaaag ttctggacca cctttttggg gaaacattcc agcaaattca
    19621 ccagagatca atgagattgg aaaagcgtac aataatactg caaccagcat gtaaaaaatc
    19681 atcgtccagc tcgttgctgc tacatctgga atatttcgta cacttgctac aagggcgcag
    19741 gtcattccga taaaggtcat ggtcgtgatt tgctttgatg aaccagtatc tttcatcttt
    19801 attccttctt tcaatagcga catatgtttt tgttttcttt tatttttgtt caagaacaga
    19861 aatgacaagg tttcatcgat tcagtacaaa acccagtatc accgaaacat ccattgaacg
    19921 atcttagtga actgggtttg atacatgaaa cgaacgatcc aacttataga acgttttata
    19981 aaaaagaata tcttggcgag gaatatatga aaaacagggg gaaatacaat tgttttgatg
    20041 aagcaaataa ttgtctgttc tccatatgtc tcttgttatt ctttcacaca aaaatcagtt
    20101 ctattggatg atttcgttta gtttcttttc cattgctttg gcgatttttt ttgtataaaa
    20161 attgaaaatt cgatcatcat tcaaataagg agtcatgata gccgcacgta gcagagtgat
    20221 tttctgttct tttttccatt cttctttcgt tagacccatt ctttcaacaa aacgaatcgg
    20281 agaatcaccg taatcttctt gtgtaaatgt agtatgagaa gtaataaacc gttcaccata
    20341 aacatcgcca tccatataag aagaccgatc gaacattttt tcatttaatt cattgatagc
    20401 gttcaaatct gtacagcctt gttctttgaa tacccaattg accatattaa aatcaggatg
    20461 attcaatgga tagacttcga ttgtttttcc tttaacagta aaagtcagat gatcgagaaa
    20521 ttctctgaat ctttgagctg cctcgattga agccccaatc aattgaccat atccggtcac
    20581 attcaatggc aatacacgat gtgctgtcca gactgcggcg gcggttgctc cagcttttga
    20641 tccttccaaa atataagctc ctaacatatc tggagccttt actgattttt caaacacata
    20701 tggcgcgaaa taagaaatga tatttctcat atttttatgt ttgattacga ttcctccagc
    20761 agcataaggg acatagccca tcttatgagg atctatcgtg acagaatccg cttctgaaat
    20821 agctctgaac ccttcatata cctctttgtc aatcgttacg cagtggtgga aaacatgatg
    20881 ttcttcaaag aattcagcta aggaagcata cggcacaaat tctcctgctt cgtttagaaa
    20941 cagtgaacgc gcatagccgc catatgcggc atcaacatgt aaatagaaat agatcccttc
    21001 atcttttaac ctttctctta attggacgat tttatctaca ctgtctactt gaccttcttc
    21061 tgtcgttcct acgacagcaa ccacacctag aattgggatt ttttgtccag ctaattcacg
    21121 aattgttttt tccaaagcat taatatccat tcgataatcc tcttgaactg ggatagcaac
    21181 catctgatct aagccgactc cgcaaatatc taacgccttc atccaagaat agtgttttgt
    21241 ttgagggacc agccatttcc ctaatctttg gatatttttt ccgctacggg aagaagctgc
    21301 tttgacctca tccatttctt cgtccgtaaa gctttcggtc atttccaaaa tttcttcaac
    21361 agataaattc aaaagttccc actcagacat ttttttcact ttttcaggca gcacttcctt
    21421 gacagctaaa ggaatcgatt tgatgcaacg ggcgtaccat aatccttcaa gattagcaat
    21481 agaaccatct gctgcaatgt gtccccatcc gtctgtcata ttgaataatg aagcaaaatc
    21541 ctgtcctact tctgcttcca tttgcgaagt tgccatagaa gattccaatg caacattatt
    21601 tgggttccac agcatcgcat agttgtaagc taataaagca ggcatcaatg tttcagcatt
    21661 catttgtccc cagtaacggc ctgctgaatg ccatgggatc gatcctgcgc gtaatcgctg
    21721 actgacttca tccaagacct ctatcatatg gtcgcgggtg gctagataat ctggagtgag
    21781 cttatctcct tcgctaattg caggcatatc tgaaggaata taattttctc tccatccaag
    21841 atgttcatct accatcttgt tcaacaacat tttataaact ggtccatttt cagctttgtc
    21901 gcctataaag acggccttga tatccatatc tttcattttt cttccctcca tattttgttg
    21961 gtcaatgtct tgcagataca tgtgtttatc tgtaacacct cttcactata gcgccaaaaa
    22021 tgagaatttt ttttttataa catcaaatcc acgtagattt ttacaaacaa aaataaataa
    22081 agtcggattt ttctgataat tcaacgcaaa aaagcgaaca atttttcaat aaatcagtct
    22141 tctaaaatag ataacctaaa aaaagcgaac aaacaaataa aatgactgtc cttcattcac
    22201 taaacatatt tttatttttt tatattgtta aaaattcgca atttattttt taatagaatt
    22261 atctgttttc caaattttat aacataaaaa acctttttta acggcattta tatgcttttt
    22321 cagaaattaa ctgatgattt caaaaagaaa acatgtcata atatttccca tattgtgcaa
    22381 aaaaatacaa ggcggaggct ctactatgat tgaagaatat attgagaaaa atattttaag
    22441 acagctgttt ttatgtgggc aattctatgt aaataaagaa gtcaatctag aaaagctctc
    22501 caatctcctg catgtttgca aaaccacttt actcaatgat atcaataaca taaaaaaaga
    22561 atttgaagaa cagatcgctt atacacatcg ggaaaaggac cgttacactt tatatttttc
    22621 agaacatata cctcgatgca agatcatgca gcaattgtct caaaattcgt tgtttttaaa
    22681 aacttgtctg ctttatttgg aagaagatga acctgattat cttcaattaa ctgaatgtga
    22741 attcatctct gtcagcaaag cttattcatt aaaaaaacaa gtattagctt attttaatga
    22801 ctgcgggatc gaaatcgacc gctactcccc tcgattcact gaaatggaaa gacgtctgct
    22861 gcttcttaat gtgtcgtatc gtttaggcgg ttttaattct tgggagctac ctgaatcgtt
    22921 ttttgaacgt gccgatcgtt tcattgaatc cgtaacggaa aatagtggca gattttatga
    22981 taaagaaaat aaagaaatct tatcgattgg ttttgctatc agtttcctac gccagcaagt
    23041 gtgtgctgtg acaatagatt caaaatttat cgaagaaatc aaaaaacgtc ctatttacaa
    23101 ttatgtcgaa tcagcatggg aaaacactga tttccaaacc tattataaaa aagaagaatt
    23161 cgcctttatt ttgacactat tcaatctttg taattatggt ttccattctt atcagttgat
    23221 tgcggaagac tttcaacagc tacaccaagt gtttattgac aatactcccg aaataaaaga
    23281 actagttgct acctttgaat ctcacttcaa tcaagaatta ttcggaaacc aaccgttcga
    23341 acgtgcgctg attcatctga tgcgttcggc ttgggataac tatcaattat tcatgccaga
    23401 aaaattttat cttttaaatg aggaacagac aaatttatat aaagaagtcc agacaatttt
    23461 ttccagctgg tccagccagt taccttatga tctacggttg aatcctaatt gcatgcgggc
    23521 ttttgtgatc gaattgagcg gaattctccg tctgacaaaa gaacatctga ctatctatat
    23581 cgtcacaaat tcggatgttc attatctgat ttatcgggaa gccttggaag ctgtaactac
    23641 tttcgatttt caagttgcac caacaattta ttcaagtata tctgatataa agaagtatgc
    23701 gcagcagtcc tcaaaccggg tattatgtga acgtacatta tatacaccag atgcggtcca
    23761 atatgaaaat attattccta tttcgatcaa tacgatcgat cgagctatca tttcagctgt
    23821 gcaaaacaaa taaggggctg tgacaaaaat catgtcacag cctcttattt gttgaagatg
    23881 cccatttctg taacaatctc ttgatacacg tcaggctcac aacctcactt attttggaca
    23941 tctacgggtc cgatctcttt atttaaaaaa taagaaatga tggttcggat aactaccaac
    24001 gccgctagtt ggaaaatatc ctgcaaggta ggtttgataa tcgattcgat gatcccagct
    24061 gaaacaagaa tttccaaact tagaagtata tagcctccca acatcttttt aatagcattg
    24121 ttctcgctga ctcggtcgct ttgcactttt gtatggacaa ataaatattt caaaaaatcc
    24181 ttgattgata gacaaacacc ccaaaccaat aaacagatag caaagatatc aagtcccagt
    24241 acaagttttt cgaaatatgg aagaagaaac tgcatcaact ctgtcgtcat cctacctctc
    24301 ctcttctttg tttttctttg tcttctattt cctgattcaa gaaataagaa atgactgttc
    24361 gaatgataat aataagtgca agcttcaaaa tatctgtcca agtcggcttg ataactgact
    24421 cgatgatatc agcaacgact agtacttcca agcttaacaa aatataactt gctagcagtt
    24481 ttcttgtatc tgcattacga gtattacgtt ctgcatagtt tttcttgaca tgagaccaat
    24541 caaaaagttt gaatagaaca agaacgatcc cgacaattaa gataccgata gaacaaatat
    24601 ttaaaatagc aatgatccat tctagaaatt caattaaatt tgagcgaaac atatacgcta
    24661 cctactttct tttaatttgt tcctattctt ttattgtaaa agaaagttac cttgttcgtc
    24721 tatttaaaag ttgttctaat gataacggtt cgcttaaatt tttctttttc actctcccat
    24781 ttttctccct ccatccataa atcgtggaag tatccaaaac aacgtagcaa gttaaaataa
    24841 ggcttagtcc gtcatcagct tttcaaagaa gcactgtctc ggtgcttttt ttgtacccaa
    24901 atttctaaaa atagaaccat acacctcctc aaatcttttc ttcttaccta ccctattttt
    24961 atttacagga aaatcagaca cttcttcttt tttgtcaaaa tttccttttt cttctcgtcc
    25021 tatcatttgg cattctaata aaaacgaact atccttggat atagaaagag aaaacaagga
    25081 ggcgtttttc atgagtaaaa aattaatcaa agtcggcatc ggtttaggtt tgcttgccct
    25141 tggcgctgca tatctaggaa aaaagacagg actattcgaa gatgacagtc atttatatga
    25201 tgaattcgaa tcaatctagc atcctattta gttgaatcac tacggacata cttggaagac
    25261 attggaggaa tcacaatgag aaaaagtaaa gcaataatga ttggtgccgg tattgccaat
    25321 atggcagcgg ctgtttattt gattcaagaa ggcaaatgga aaggggatca gatcaccttt
    25381 tattcattgg atgatcacgg ttcaaatgat ggtgcaccga ccgacactgt agtggatgaa
    25441 tattggaata aaaaccatcc attagaaaac caaaaaggct atattgctcg aggcggtcgc
    25501 atgcttaatt accgtaccta tgtagatttg atggatctgt taagtcgaat accttctgtt
    25561 actgagccag ggatgactgc tgaagaagat acgagagatt ttgatgcaaa acaccgaacc
    25621 tttgacaaag cacgtttgat ggaaggtggc aaaggaatca ttgatggcgg aaaactggga
    25681 ttcaataata aagaccgtat tcttctgact aaactgattg ctatgcctga ttcagaagaa
    25741 gaaaaactag acaatatcac tatcgaagaa tatttcaaag atgatccaca cttttttgaa
    25801 acaaacttct ggtttatgtg ggaaacaaca tttgctttca gaacacgtag ttctgctcaa
    25861 gaattgcgtc gctatatgca tcaaatgatt tatgaattca ctcaaatcga acatctagtg
    25921 ggcgtcaatc gaacacgtta caatcaatac gaaagtatta tgcttccttt gatcgagtac
    25981 ttgaaagaac aaggatgcaa aatcattttg aaccgccgtg tcactgactg ggaattcaaa
    26041 gaaacaccga tgcaagatga aatcactgtg actggtttga aaattaccaa tacactgact
    26101 caagaagaag aatacgttgt ggtcgatgaa gatacagcag ttattttcac taatgggtcc
    26161 attacagatt cagctacttt aggagattac gaaacaccag ctgtagaaaa tatggattat
    26221 ggtgcagctt ctagtctctg gaaaaaagcc agtgaacgtt tctacaactt gggaaatcct
    26281 gacaaattct ttgctgatcg agatgcaagc gaatgggtca gctttaccct gacgacgaaa
    26341 aaccatttgt tgttgaatga aatcacccgt atcacgactc aagaaccagg aaatgccttg
    26401 aattcattta tttcaaccac accgatcacg ccgttaggcc aaaaagatgt caatatgtct
    26461 attgtcgtgc atcatcaacc gcattttacg tctcagaaac caaatgaaac agttatttgg
    26521 ggttatttcc tttatccaag acgacgtggc gaatttgtgg ataaacagta tattaaaatg
    26581 aatggaaaag aaatgctaga agaactgatt ggacaacttt caaaagtaga tcctggacca
    26641 gtgaatattc gtgaaaaaga aacagaaatt ttcgacagcg taataaataa tatccctgtc
    26701 tatatgcctt atgcttccgc gttgttcaat aatcgtgcca aaagcgatcg accaaaagtc
    26761 attcctaaac attcgaccaa tctggccttt acaggagaat ttgtcgagca gccttatcag
    26821 atgatcttta ctgaacaaag tgctgtacgt tctggagaaa tcgctgcttt ccattttgcg
    26881 ggtattccta tgtcccgtct tgtcaaaaca ccgcgttacg acaaagatat tcctactcta
    26941 gctcgtgccg caaaaaaaat gtttgaataa tcaagaggtt gtgaaaaagc tgccctgcat
    27001 caagtaataa gaacaaccag gctaaaacag cttcttatgt ttttgatttg gttggactta
    27061 ttaccgcaga tgcaagcttt tgaacaccgt ttaccaagag gttatgaaaa agctgccctg
    27121 catcaagtaa taagagcagc caagctaaaa cagcttctta tgtttttgac ttggttagac
    27181 ttattaccgc agatgcaagc ttttgaacac cgtttaccaa gaggttatga aaaaggcgtt
    27241 tagtttcgag tatgaagaaa aattttgtga aaatggttcc tcacgttttt gcataaattt
    27301 gacttaatac cgagaaactg atcccttctg tcataacctc tttttattta tgtctgtttc
    27361 tttttgaaaa caactatttt ttcaacgttt actctcttgc caatccaaca tcatctaacg
    27421 gatacaacta tttcaatgat taccattaca ctaaaaaatc ggaaacacac ctctccgaac
    27481 acaaatacat aagactatag aatgtttcat ttaagaaaaa aagcgtttca ttatcaaaaa
    27541 ggtaagatct cctttaaaat gaaattatct gtagtactaa taatgcagac aattttatga
    27601 atctgaaagg agcatctcca atgaaagcag tcacttggca aggaaaacag aaaatggaaa
    27661 tcagaaacgt agatgatcca aaaatattag aaccaacaga tgcaatcatc cgtatcacag
    27721 caacagccat ttgcggctcc gatctgcatc tttaccatca tggtgaagca gttttggaaa
    27781 aagggtatgt tgtaggacac gaaccaatgg gaatcgttga agaagtggga tctcaagttc
    27841 aaacattgaa aaaagggcaa cgtgtggtta tcccttttaa tattagttgt ggtcattgtc
    27901 atttctgcct gaatcacatg gaaagccaat gtgatgaatc aaatccagat cagttatatg
    27961 gcgggctttt cggatttggc aagttgaacg gtaatcattg gggtggtcaa gcggaatatc
    28021 taagagttcc ttatgcagac tctacttcat ttgttgtacc agataatgat cttcctgatg
    28081 aaaaagtttt atttctatca gatattttgc ccacagctta ttggagcgtt gaaaacgcag
    28141 gagtaaaaaa aggcgataca gttgtgattc taggttctgg tccagtcggt ctattcgctc
    28201 aaaaattcgc tgttatggct ggagcagagc gcgtaatcgc agtagatcca gtacagcacc
    28261 gtctagataa agcggctaat tacaatgggg ttgaaactta tttcttagaa gacacggcac
    28321 aagctggaac agaattgtat gaactggcta aaggtggcgc agatgtcatt atcgattgtg
    28381 taggaatgga cggattagag cctgtcaaag aaaaagcgaa aaatttagta agcttacaaa
    28441 gcggcacaat ctctccatta cagatggcat cgcaagctgt caaaaaattt ggaacagtcc
    28501 aagtaactgg tgtttatatg actccagctt cttcttaccc gctgcaagaa ttttttatgc
    28561 gggatatcgc tgtcaagcat ggacaggcac caattatcca tctaatgcca aaaatttacg
    28621 caatgattgc agaaggacta ttcgatccta cacaaatcat tactcattcg ctgccactat
    28681 cagaagcagc acaagcatat gagatctttg acaaaaaaga agataaaaat ataaaagttg
    28741 ttttgaaacc ttgaattcag taaaaaatac aggtgatttt ctttatgccc aaaatcaaac
    28801 gaaataccca tgagatcgaa ataggaaaag aagaagatat cgaaatacga ggacgtcgct
    28861 tccgcttcta tttccaaaat cgttacacat tgatctcact ggctgtcgat ttattaaccg
    28921 ggattttcta tatttttgga agccttgctt ccttaactaa aatccccgat cgatatggca
    28981 cctattttta tttggctggt gctattttct tgacgattcg tcctattctc agaatcgtaa
    29041 ggaacatttt tatttacgac gaaaaaaaag caatgaagag aacaagcaaa aaaaaagagg
    29101 catgagaatt tttcaaatct catgcctctt ttttcaccct atcgtatcac tttgatacag
    29161 acgagcggtc gtcccacttt cttccttgac cattgtcaaa aatgtccact cgtatcgttc
    29221 ataaaacgtg gtatgatctg taattaagta aagtttttcg tacccaagtt gatgcatttc
    29281 ttttctgatt tccgctaata tccgcttggc tatcccctgc ttacgctttt ccggttcaac
    29341 aaacagggca caaagatttg gtgtcagatc agggcggtta tggaagtcgt tttcaatgac
    29401 gcccgctcct ccgacgatgg tttctttttc atctaagacc acataccatt gaggaatact
    29461 ttccttatta gcgattccag tacgaatact ttcctcatac acaacagcag gaactcccca
    29521 ttttgatgaa aaccaattcg cgctttgttt tactaattct gggtgttctc ttaatttgat
    29581 ccagttatat gtaaccaatg tttaaacctc cgactaaaat aaaacgttta caaaaattat
    29641 acaagtcttt atgaaaatta gcaatgacaa caatagtgat gtaaaaaaaa aaacggaaaa
    29701 caatcacttg ttctccgttt tttgattaat caagcatcta aagcttcttt tcttctttct
    29761 tggatcgtag ctaccttgac gaacggtaga tagatcaaaa tagaaagaac caaattgaca
    29821 gcggctagta caccacctgc aatacttttt gttgccaata caccgccaat aattggtgga
    29881 gttacccatg gaggcataac ggtagctgct ggcactaaac ctgtagaagt cgcaaagtaa
    29941 gcaacactaa ctaagaccat tggtgtcaaa ataaatggaa tgaacatgat cgggttcaac
    30001 acgataggta acccaaacat cgttggttca ttaatattga agacacctgg cgcaatcgac
    30061 aagtttgtta caaccatata tggtttgttt ttacgtccta ctaaataaat agctaacagt
    30121 aaacctaaag ttgcacctgt tcctcctaag ttgacaaagc tgtcaaagaa aggtttgttc
    30181 acgataaacg gtgcgctatg tccagctgcg attgcattaa cgtttgcttc aatggctggc
    30241 gcattgattg tttgcatcaa aggatcgacc atattcgctc cgtgtaaacc aaagaaccat
    30301 aagaatggtg tgataaatgc aagcagtaag gcagatggat aagagtttgc aagtcccata
    30361 aatggttctt ggaccactgt atagaaagaa cctacgatat catcaacacc gaatgcagca
    30421 aaaattgcag caactaaagc aaacgcagag atagtgatca ttgctggtag taatgaagca
    30481 aatgcttttg caactgccgg tggcacacca tcaggcattt tgatgatcaa tttttcattt
    30541 ccgcttaaac gtacaaagat ttctgttgaa ataatcccaa caataatagc aataaacaaa
    30601 ccgtttgagc tcatacctaa tgctccgcct aaagcgaaga aagagcctaa agaaatcaca
    30661 cctgcggaaa caccgtcttt tcggtagccg ttagctaaat gataagcaag caagaaagca
    30721 atcaagacag aaagaatggc aaatgtaccg ttccaagcag cagccccgaa tcctttccat
    30781 gattcaccac caaaaattga gttcatcaat tcttggaacc cgggaatcgg taagttattg
    30841 atcattactg ctaatgctcc taagatcatt aacggcatac tcaccataaa tgagtcccga
    30901 atcgcaacta aatgccgttg tgcaccaatt tttgatgcta caggtataaa atgtttctcc
    30961 ataaatgcaa caaatttatc cataaatctt ccctcttttc tttactgtag attacgcgtt
    31021 caatcgacga tacagatcaa tgatctcttt tgctaaatct gtaaaagcta tagatgtcat
    31081 cagatgatct tgactgtgga ccgtcaaaag cgtgacttca acgtgatttc cttgtgcttc
    31141 ttgtgtcagc atctctgttt gagagtgatg tgcttggact aaagattcct ctgcatcttt
    31201 gattttttca tctgctaaag tgaaatcacc ttttttcgca gcagcaatcg cttccattgc
    31261 atcgcttttt gcatttccgc caaacatgat cagacccatg atggcttcta gatttttttg
    31321 atcttccatt tattgtatcc tccgataatt tttattattt gtttattagt ttttctgctt
    31381 ggtcgagtac tttttcacca ttcatcatgc catagtcagc catattgatt acatctaaag
    31441 gaatgccttt aggtgctagc ttttcagcaa attgattttg cataaagcgt acttgcggtc
    31501 ctaaaagtaa aacgtctaca tttttagatt ctagttgatt atccgcatct gaagcagata
    31561 cagcaaagat gtctgcttcc attccttttg cttctgctgc cttttgcatt tttgtgacta
    31621 ggagacttgt actcattcca gctgaacata caagcatgat tgttttcttt tccattttaa
    31681 acacgttcct ttctatactt acacttgtat ataagcaagt agcgtgccaa ctttaaaagc
    31741 gctttaaaaa gttgatatca tcacatttaa ggtctatacc aagttgcata cacactaaaa
    31801 aagaaagcgc acactaaaca cacaaattaa tttttattac cttttttctt ttaacgtgca
    31861 caaaaaaagt tgtgacacaa aacttgtcac aacttttttc aaataaacgg tattcaaaag
    31921 cttgcatctg cggtaataag tccaaccaag ttaaaaacat aagatgttgt ttttactttg
    31981 ctattcttat tacttgatgc aggacagctt tttcacaacc tctcaataaa cggtgttcaa
    32041 tcagctgacc ctgggtatta gctcaaaacg ctggattcac aacctctttt atttcaaata
    32101 accagtaaat acaatgaaca tctacaaaaa acttctattt atttatagat ttcgtcaggc
    32161 aaatccaatt ctctcttatc aatcaaagtc tgataccacg aagcagattt tttcaatgtt
    32221 ctgcttcgtt catcgttcaa ttcgattcga actaatccat atcgattctt aaatgcattc
    32281 attggtgaca cacagtcagt gaatgcccat aacatatagc cttcgcaatt tgcgccttct
    32341 tctgttgctt tgatcaagta atgcaaatgc tgtccgataa attcgatacg gtaatcatct
    32401 tgcacgattc ctttctcatc catatactgt tcttcatatt ctcttcccat accgttttct
    32461 gttatcagcc aagggatatt atgataattt tcttttagat acatggctat atcatacatg
    32521 attttcggat agatttccca tcccctggaa ttattcattt tcttcccagg taaatcaaag
    32581 acttcataat atcgttcagg atggaaaggt gtctcttcat tccattgata acgaggggcg
    32641 cgaacgcgtt taggataata ttgattgatt ccaaggaaat ctactgtatg atgctgaatc
    32701 gtttctaaat cctctgttgc aggatcgaaa taaatcgatt gtttttgaca caactccagg
    32761 aactcttctg tgtagtatcc ttttaccatc ggatcaaaaa atactcgatt ataaaacaaa
    32821 tcatacatat aagcagcttt ttggtcttct ggagaattgg atcgagcata gaccatttca
    32881 ggatttaaaa tacagccaat ccttccacca taatttccgc tgcggtaaat ctctactgct
    32941 ttagccgttg ctaaaacttt atgataattc cacatcatcc acttttttgt gttttgttca
    33001 tgcggccagc gaacagcatc caaatagcaa cgcgtttggg gaacgattgg ttcgttgaat
    33061 gtaaaccagt tttttaccct atcaccgtaa cgatcaaagg caatttgagc atacttagca
    33121 taaaaatcaa ctacttttct tgaagaccag ccatcatatt tttccaaaag aaaagctggg
    33181 acttcataat gttccaaaca aatcatcggt tctacgtctg cttcgatcaa agagtcgatc
    33241 atttcactta tatgctgagc atattcttca tctactatac agttctcata atcagtaaaa
    33301 aaacgagccc aattgataga agtccgataa tgtgtcagat ggatggattt catcagatcc
    33361 acatcctgct ggtattgttc cataaaatta gttgctacgg ctgggccata cccattatgc
    33421 caaacaaaat gttcattttt ataccagcta tctataaacg aatcctgtcc ttcttttttt
    33481 cctttccaac cttccgtttg ccaagcagaa gatgcagcac caagaataaa attttttgga
    33541 atagtaattt tcatgatttc ctcctgtttt ttagatccca tcattttcca cacgatttgc
    33601 tacttttaca aatggcagat agatcaaaat agaaacaata atacaaacaa gttgcgtaat
    33661 aactgcaccg atatttcctg ctgttgccag ataagcattg acgatcggcg gcattggcca
    33721 agggaccatt acaactgctt tcgccgcaaa tcctaaagcg gttgcaaaat aaccaatcgt
    33781 tccagtgata agaggcgtta agataaacgg aatagctaaa attggattca gcatgatcgg
    33841 cataccaaaa ataactggtt cattgatgtt gaagatccca ggagcaaggg ataacttggt
    33901 gatttgtttc atatcttccc gttttccaac taacaatact gcaatcagta aaccgatcgt
    33961 gactccagac ccgcccatac tcatatacat atcccaaaat ggcatagtaa taatgttcgg
    34021 tacttctttt ccagcttcaa aagcttccgt atttacagca attgccgcta aaagcaacgg
    34081 ttcccgtatc ggcttcacca tttgatttcc atggatacca atcacccaaa agacttgcgc
    34141 aataaacatc aatagcaaaa tacccggtaa cccttgtacg acaccttcta atgggcgttg
    34201 tacgacattg taaatgatat cataaaggta cattcctgta agttctttga tgataaatcc
    34261 cgcagccgcg atacccgata cagtcagaat cgtcgggaat aaagcagaaa aactagcaga
    34321 aacattcgtt ggcacggtat caggcattct cagctgtagt ttcttctgtt tcctcaacca
    34381 gcaataaagt tcaatagaaa ggatggagac caacattccc aaaaataacc ctttcgtatc
    34441 tgtatattgt ttagccaata cgttttctac tataacagtc tgattttcat tcactaattc
    34501 ataaacagtt ggattaacag ataaataaga catcactgcg atgataccag ggaatacacc
    34561 atccacttta ttcagcttgg ctaattcgat ccctatcaga aaaacagcat agatggtcaa
    34621 gaaacttagt gtgacatagc tgatggcaga tgcgataggc tttaattctt ctaaaaaacg
    34681 caagaaactt atctgtgcta agccattttc cgagtcaaag accattgctg aaaacaatgt
    34741 gccaaaagca cccgtaataa ttaccggaat caatgcagca aatgcgtttt ttatcaccat
    34801 gatatacctt aaattattga ttctcgttgc cacgttccct aaaacattgg ctaatttatc
    34861 cataaaactc attccagatc ccctcattcc tgcttcttct ccttgacgtc atctcttttt
    34921 gtctattagt cagcgcttac atttcgatga acagaaacta tttactcctc ttttttacca
    34981 aatataaaac aaaaattcat ctgatgtatt tatcataaaa tacatcagat gaactgtcaa
    35041 ttagtttctt tttctcctat tattttctgt aattctctcc gatttattgc catatggaca
    35101 gtcattgcat caaacgcagc aagtgcattc cgcctttcga tggcctgaac gatctctgta
    35161 tgaatatcga tcgtctcgat tttcagatgc tggatatcat aatttttatt aaacaaagca
    35221 attgattcat taatgattgg gataatatgc agcaaggcta cgtttccgct agcttctgca
    35281 atcatcgtat gaaaatcgat gtctaattga acatgttctt gtgtgccttc tcgaaaagaa
    35341 gtttcaatct tttgcgttaa ttttttcatt tggacgattt gattcttcgt tgcatattcc
    35401 gctgccaaac ctgccatctt aggctccaat aaatatctca gttcaaataa gtctcgtatc
    35461 atccgatcct gatcttcaat caaagaaaaa ccgagtgggt cattggcaat ccctcgctta
    35521 tcactgacaa atgtccccgc tccttgccga acttcaagga tattcctgga agctagagcc
    35581 cgcacagctt ctcgcaccgt gcttcgcccc acctctaaat aatcggacaa ttcatattca
    35641 ttcggcagtt tatctcctac ttgcaactgc atttccttta aatatctaag gatcttgtca
    35701 gtagttactt ctactagggt tttattttct tccaaaccac atacctcctt ttcatctgat
    35761 gtattttttc gtaattatta taacacgttt gaatcatctg atgatttttt gtatacaaaa
    35821 ttgattaatc cgttcataga catacaaacg aagaagatat ttaccataaa aaagactagg
    35881 ataaaattca ctcctagcct tcgatcattt ttattttgaa cgacaaaaaa tatgatgttc
    35941 aataacagac gatcttcatt cgattcttga tgtcatcttt tgtttttttt aatatatgga
    36001 taaaaagttt gattacatcg tttctattct agaatttggt cgattctcaa ttcttcttct
    36061 ggtgagaaat ctaaattatc catcattcga acactttctt gtaactgact taaccgactt
    36121 gctccgacca gtacacttgt aacagatttt tgtcgcaaat tccatgcaag ggccatttgc
    36181 gctaatgatt gtcctcgtga tacggcgatt gtttgcagtg ctttgatttt ttccagagtt
    36241 gatccaactt gttcttctga aaggaatggt atttccttac gatgtgctct agaatcttca
    36301 ggaataccat gtagataacg atttgtcagc aacccttgag caagcggact gaacgtgatt
    36361 gcccctagcc cttcttcttc taatactgga gatagatcgt cttctagtag tgcacgagaa
    36421 aacatattgt agcgcatctg atgaataatg aacggtgctt cctttctttt taaaatttca
    36481 gtcatttttt ttgtatcttc tccattatag ttggatatac caatatataa tgcttttcct
    36541 tgtcttacaa gttgcatcaa agcttctgct gtttcttcca aaggtgtatc agggtctggg
    36601 cgatgatgat agaagatgtc aacataatct agccccattc tttggagact ttggtcacaa
    36661 cttgaaatga tggatttacg agagccccat tccccatatg gcccttccca catatgataa
    36721 cctgctttgc tagaaataat cagttcatca cggtaggctt gaaaatcttc tctaaaaata
    36781 cgcccaaagt ttttctctgc agctccagca gggcctccat aattattcgc aaggtcgaaa
    36841 tgggtaattc ccatgtcgaa tgcgcctctt aaaatctctc tttgattatg gataggatca
    36901 taatcaccaa aattttgcca taatccgaga gataaaatcg gtaatttcaa acctgaatga
    36961 ccggatttac gatattccat tttttcataa cgtgcactat ctgcaatata caatttttct
    37021 cctcctcatc aaatcttttt aaaacgcttt cttttttcta tttttagttt actacatttt
    37081 aaagaaaagg agttaatcaa aatgattttt tcgtatattt ttttgccatt tgttttttac
    37141 tcgttgatag atcagaaatt gattacgtgc ttgctggatt tttttataac gtttcggttg
    37201 ttttttgtaa aattgctgat gatagttttc tgctggccaa aacgtaccgg ctggttcgat
    37261 ttttgtgacg attggttggt ggaaagttcc tgaactgtct agtttctgtt ttgattgttc
    37321 cgctaattct ttttgtcgtt cgacttgata aaaaatcact ggacgatatt gcttccctct
    37381 atcttgaaat tgtccaaatg tatctgtggg atcagtaatt tgccaataga ttgtcagcaa
    37441 ctcgctataa cttatttctc ttgtgtcata aatgatctcc acagcttcca catgacctgt
    37501 gtaaccacca cttacttgat cataatttgg tttttcaaca tgtccgcccg tgtatcctga
    37561 caaaacagat aggatccctt ttttcgattc aaacggctcg accatacacc agaaacatcc
    37621 ccctgcaaat actgcacgtt cttgatagtc agcatcttgc atggcatgct tggaaaagtc
    37681 atatttttgc tccttatcaa atccttccgt aatcaataaa taaaagtcca ttacatcagg
    37741 cgtcagattc attcggatag ctaatggacg tagtgttgct tctagttttg ataactgttc
    37801 tttaacggat atattttctc ttatgtcatt ctttgtctga atcaatactt ttcgttccca
    37861 atctcgtgta ttcggattca aaatcagatt ataaagtgtt tgaatgaata tttgttcctc
    37921 atccatagat tctcctcctt tccgaagaag acaagctagt gactttagct gtaatttatc
    37981 ataaagtgag gaaataagcg attacttcat ccccctacag atttttcgta taaaaaagag
    38041 agaatcctta tcgacagatt ctccttttta atcaatttat tttataaatg aaaagttaat
    38101 ttacctttta tggaaaacta ttttaaaaaa taaacataaa tatgcagtta atgtaacaag
    38161 ataagaaata atagtcgtta ctgtataact tgtgcttact ttaatatcag caactccttt
    38221 gactccgctt atttgtacca atccattagc attcacctgc tcctttacag gttgatgatt
    38281 tatttctaca ttcgttccta aataagcata tacaggaagt gtgattactt ggctcttctc
    38341 attagttact ctatatatga tagaactacc agttgtttta taaggaaggt ttacccattg
    38401 actgccaatc tttgcttcgt gttttattat atgctcctta ttctccaatg aagcgatagg
    38461 agaataatca tataaaaaat aatccattcc tttagtggta tacgcctcta ctgtttcttt
    38521 gtttacaaca atatgtcccg gctgagaaat tattttttga ttgaaatttt ttttactaga
    38581 aacagttaaa gtgattaata acaaaacagt cataaataaa taaaatagtt ctcttgtctt
    38641 attgtatctt ctatttttga aaaatatcat cgttaatatc agagaagaga atattatttg
    38701 caagcttaag atccgccaag gaaattgtaa caatttggct ggcgtatttt gcagaagata
    38761 ccatggcacc aaactagttg tagctagcca agtcaaacac gtaccataat aaatcttttt
    38821 ttctatgttt gttaattttt taaataaaat caggggaata cataaagata acagaccaac
    38881 tagtcccatt gagtaagtag ttaagtcgtt gttcatagct tcagtcagac tatcgaacac
    38941 attagatgca tgagcatata aatttgtcac gctaggtttg ttgatttttt ggtagagcat
    39001 ttgttcaaac atcggatacc aaaaagcact cgtcagaacc aatgtcattc ctgccgcact
    39061 aaataatttt ttaaatatag caataaaatc tgcattcttg aaacctttag aaaccaaacg
    39121 aataaaaata aaaatgataa ttaatgctgt actcattaat gttgatagta catggctgta
    39181 cactagcaaa ctcatactca atgctagctt tacagacgga tacttttctt cttttccaga
    39241 gatgatttga taagcataaa ataatacagg gataacaagt gttattgcga tagcttcggc
    39301 aattgcggaa cgatagtaga tgtctactgt ccgataagca gagaaaatat agagacaact
    39361 aaataaaaat gccgcttttt tttgttttaa aaaccgctcg ccataatgat aagctacgag
    39421 tgttgtacac aagttcaata accaaacgta aataatatag cttacaatca aattttttgt
    39481 catccaatat aacattacag ctggaaaaaa agtcagaaag ggataaaaat aatttacacc
    39541 atatccagta taattgaatg tctcataatt aatcggactg gtaaaaatag tcgataaccc
    39601 cttgattcga gataagtgaa aagaaagatc tccagtggta taaatactct gtgtattttt
    39661 gaaaaagatg aaagaatata tactactaac tataattaat ccactcaata ttactacttt
    39721 tttatttatt ttcattcttc ttgtttatct cctttttagt cacatatcga gaatctttca
    39781 acttattagg tgcaaatcgt ttaaaacttg aatacaccac taaacaataa gtcagaatac
    39841 tgatccataa agccatgaga gcagttggag tatattttgt tgtaatttct atcttatttt
    39901 ctccagcatt tacatccaca ttcactaagc catgtttttt ataagagtca tttacgactt
    39961 taccgtttac ttttacctgt gtacctaaat aagcatatac tggtactgtc actttttgct
    40021 ttttgttgct tgccactgtg taagtaatgt tattatctgc agctttatac ttcccttttt
    40081 cccacgattc tcctactttt acttcatgtt tttctaagtg aggcctttct tttatcgctt
    40141 ctgttggggc gtaatcgtat aatcctccca tatctgatgt tgtataacgt gggagtgttt
    40201 cttcattgat aacaatatga ccgtgttttg attggataac ttttgcgtaa ttttcttttg
    40261 ctgctataga cgtgacgagt agaaacacca cagtagcgcc aatgattaac catgaatatt
    40321 ttttattcat agtctttttc ataaaaacaa gcgttatgat cattgaagaa aaaaagattt
    40381 ggatacctaa gatacgccaa ggaaactgga gtaagcttat cggtgttttt tgaaacaaaa
    40441 accatggaaa taaattagtt acgcataccc atgtagaaac tacgccaaca tagatgattt
    40501 tttctttttt ctccatttct ttcagtatga ataaaggaaa aatcaacgca accaatccta
    40561 ataaccccat agtgtatgtt gtaatatcgt tatttaacgc ttgaatgata ctatctgaaa
    40621 tagaaagtgc ttctagctgc aaatctgttt tgaagggact gtgtatttct tgataattca
    40681 tctgctgcaa cataggatac caaaaatagc tagttaatgc acaagtcaaa aaaacagcct
    40741 ttactaaatt cacgaccaca aattttatat cgttttttat ttgcttgctt aaaaacaaac
    40801 gaactataat catacaagca atcaatgcac tacacaatag tgtagttaag acatgagtat
    40861 acacaagtag acttatactg acagataaag cgatgacttt tttttctcta ttgaatatca
    40921 atccataagc ataatataaa acagctggga taatcgtaag agcgattgct tctgcaattg
    40981 cggatcgata atagatatca actgttcgat aagttgcaaa agtatataaa caactaaata
    41041 aaaaagcagc atctatcttt ttaaaaaact tttctccata ataaaaacta atcagaatcg
    41101 tacaaatatt taataaccaa atatatagaa cataacttaa tattaagtta tttgaaattc
    41161 cataaaaaac gactgcagga attattgtaa gaaaaggata aaaatagttc agtccatctc
    41221 cataattgtt aaatgttgta tagttgattg gcccttcaaa aatagaagat aaccctttga
    41281 ttcgtgaaag atgaaaagat aaatcacctg ttgtataaat ttcttgagca tttctcataa
    41341 aatttactga atataaagta ctaagtaaga taagaactac taaaagaaat gcttttttac
    41401 ttattttcat agatttgttg ttctcctttt ttaattctat tttcagtata ctagaattgt
    41461 ctttttttaa taagttttgc gttaaatttt tcatagcact agtatgatat actagaaaag
    41521 aatcaatttt tattactttt aaataggaga aaaaaatgaa aaaaaacaaa aaccaaataa
    41581 tagatatcat ttttatgtta tttctaagtt ttgcttacgt catccctcta ttcatgtcta
    41641 agggaatcta tcatagtgtc aatcaggata cttactttca tttatctcga atcataggac
    41701 tagataatgt atggagttct ccagtaaact tcaataactt cgatcatcat ggaacaatga
    41761 tgaatatttt ttatccatgg cttactcttt atccagcttt tttattttat aaaatgatgg
    41821 gaaatcttgt tttaagttat aatatctact atttttttat cactttttta acaatggtcg
    41881 tttcttattt ttctatgaaa caaataaaaa acaatcgtta tatttcgctg ttattttcga
    41941 ttatttatac attttccgcc tatcgtgcca ttgacatatt ccatcgtgct tcattgggag
    42001 aagctgtcgc tttaacattt ttgcctttga tactaatggg gtgttacgaa atttatatta
    42061 gagattacca aaaatggtac tggttgtcta ttggcatgac gctagtagtg tatactcatt
    42121 tactttctgt cgctatggtt tctgtattta ttggaggaac gttgtttctt tctttttatt
    42181 tttgggacca aaaaatcgct agactcttaa gtcttttaaa agctaccgtc ctcacctttt
    42241 ttttaagtgc gggattctta attcctttta ttcaacaatc tagagcacaa gaattgaaag
    42301 tcccactggg aaaagaatta agtggtatgg ctcctagtga tatgctgaca catattttaa
    42361 ataataacta taacaactat actattggat tattcttatt tctcggattg attggtgctt
    42421 ttatttttat aaaaaaatta actgctgatg atttatttat tttctttctt ggtgtatttg
    42481 ttcttttttg ttcaaccaat ttatttcctt ggcaactttt taaccataca ccagttaaga
    42541 gtctacaatt tgtctggcgg ttaaatggtt tttcttcttt atttatagcc tatacgatga
    42601 gtatagtgat ctattatatt ttttctacta aaaataattc atggaaaata atggtcttta
    42661 cgtttcttgc actaatattg catctctcag gagtaagcaa ttctatccat gctaacaaag
    42721 attcattaac acttatagca cctgaagacg ctgttgctat agctgaaaat tacaatcata
    42781 ctgattatgc aaacaaagag tcaatctatc atcctgatat gattaataat gatcaatatt
    42841 tactaggtaa ccaggaaata aatccaacaa ctcattttat gagtaacaaa cttacaattg
    42901 aattagataa ttcgaataat aaaaatactg tcttgacgac acctatttat cgttataaag
    42961 ggcaagtggc ttctattaat ggcaaactag tacaaacaaa attatctaag tttggcacaa
    43021 ctgaattaac tattcctcca ggtataaata aagtcgttat tacttatcag tacacaaaac
    43081 tagcaattgc ttcaagatat ttatctatcg ttactttgat tttattttta ctttatcgat
    43141 ttactttttc taaacaaaaa caaccgagaa gagaagtaca acattctcac tagattttta
    43201 tttaagaaag actttttagc tgtacacata aacaggctaa aaagtctttt tttatatttc
    43261 tttaatttgc ttcatacatt atttaacatc gatatttata ctgtgaaaca tgcgtttcat
    43321 ccattatcaa agtattttta tcgataataa acttcatcca aaaacagacc ttgtgcaggg
    43381 acggtttctc ctgcatgttg ccgcactttg ctttcaaata tttcatcgat tgtttctatc
    43441 ggcatggcac ctgtgccgat ttctagtaat gtccccatga taatccgcac cattttatat
    43501 aagaatccgt ctccgacaaa agtaaaatga agcatttctc cctttcgttc gatagataat
    43561 tcatcaatcg tacgcactgt ggattttttt gattttttca atgcagaaaa accaatgaaa
    43621 tcatgtgtac ccactaattt ttcgcaagct gcattcagct tgtccatatc caactcttga
    43681 gggaaataaa agctgtggtt tcgctcaaat gctgaaggaa tcacgttgtt ccaaacataa
    43741 tagctgtatt ttttgcccac cgcattatat cttgaatgga agcgctcagg catttcttcg
    43801 attcttttga tgacaatatc ccgcggaagg taacgattca taaagtcgat catttctgcc
    43861 agtccaattt ctgtgtttgt tttaaaattt gctacttgtc ctcgcgcatg ggtgccagca
    43921 tctgttcttc ctgatccaat aatttctatc tcttcatttg tcatctgatg caatactttt
    43981 tcgatttttc cttgaatggt tttatcgtca tctccaagtc tttgccatcc tgaatatctt
    44041 tttccgtcat attcgattgt caacttgatg tttctcatat tttagtggct cctctgtctt
    44101 ccatttggtc ttctctctac ctttttaaga ttgtagcaca aaatatagct ccttgcttct
    44161 ctaagacaga agctatacaa gaaaactgtg gaaatatata tttcagaaaa acaaaaaaca
    44221 gccaccggat gaagtatccg ctgactgttc aatttgacgg ctcaagtttt actgtttgag
    44281 atgcaagata ctcaaccagg caatgggcac ataattaaag aaaaaactga ttctttggca
    44341 aaaacctatt ttattcagta atggccaaga atttatagaa ggaattcttg ccagtccata
    44401 aatcgccgca aaaagcaaac tgatcacaga cagtccaagg tataagtggc ttacgtccat
    44461 ttgcccattt tttcgataga tcaagaaaag aagaactgga aacaataaaa aaccactgta
    44521 acctaaacct gatccgatat tatggaccca tgtcgagaca ttccacgaag attcatgggt
    44581 attgatactg aacaaacctg taaagataca atcacctata ccatagaacg cgatggctgc
    44641 tgtaactagg gtagctaaca cacgagatgt actaacaaat gcagcataca atgcaggcaa
    44701 tgacaggatg aaaaagatcc ctgaaaccac agaccaaata agaaacgctt ttcttacagg
    44761 actggaaacc tctccaaata cactgatcac gctcttcatt tgatctaatt caggataata
    44821 caatcctaat acgtacggag tcaaaaaatc actcagcacg cctaataata aaaagaaaaa
    44881 accaaattta cgtaaaaaat ccatgatacg cctccaataa caattaccta tgcttttaat
    44941 tgttattata aacgacttta tcttgattta acagaaacct ctatttacac gaacatctat
    45001 ataactcatt aaaaactcga atcattttaa aaatactaaa tatctaacgt tcaacgttca
    45061 cttaatccat ctaaacaatc tttcaatttg attttttccc cacttctttt acgcgcgcct
    45121 agctctccta ttgcagtaaa caacagatca cttgacgaat ttaaggcagt ttccattgaa
    45181 tcctggataa caccgataat aaatccaata ccgacgattt gcatcgcgac atcattagag
    45241 ataccaaaca aactacatgc taatggaatc aataacagtg aaccgcccgc aacacctgaa
    45301 gctccacaag cagctaaagc agacaaaata cataaaagga ctttcaaaac aaaaggtgct
    45361 tcaatcccta aagaatgtac agcagtcaac gtcatcaagg aaatggtaat cgcggcaccg
    45421 cccatattga tcgtagcacc taacgggatc gatatggaat aagactcttt tgtcaatttc
    45481 aatctttcag ccaacatcat gtttatcggg atatttgcgg cagaacttcg ggtaaagaaa
    45541 gcgggaagtg cgctttcttt caaacaaaag aaaatcaatg gatagggatt ttctttaata
    45601 aacaaaaacg tgaggaatgg gtacacaact aatgcgacaa acagcatcgt accgatcaaa
    45661 acaagtaaaa gctgcagata ctctgccaat ccagcgattc cagtcgtagc aattgattga
    45721 taaaccaatc ctaaaatccc tatcggagca aatgcaataa tcacttgtac gacttgtgac
    45781 aacgctatag atatctgatc caccaactct tttgtcgatt ctgaacgtgc tcgaaaagct
    45841 agtcccaaca atacagccca gaataagacg ccaaggtaat tcgcatcaat aagagattga
    45901 acaggatttt gtgtgacatt cgtcaacata gtagataaaa cactttccaa actttgcggt
    45961 gctttttcag atactgtttc ttgtaaagta ataggaacag taaaaattga acttacaata
    46021 actgctacaa gtgcagaaag caatgttccc actaaatata agatcaaaat aggcttcacg
    46081 aatgtcttat tcccaatttt atgcttggca attgacgcca tcgtcaagaa aaaaaccaga
    46141 ataggtgcga ttgccttcaa gcaactgaca aacaacgttc ccaatattga aataaatgtc
    46201 catgtgggga aaaagatacc aaacaatatt ccgactaaaa taccaacaac aatacgttga
    46261 actaacgaca tatccaaaaa cttctctaac aaatgaccac cctttcacaa ttttaacaat
    46321 cattgtatca ttttatgcaa agaaactaaa ggtataattt tctaaaaatt ctaacaatac
    46381 aaacaaccat ttcttgctaa ttttgtactt tttctgtcta cacgttgttt tttgagatat
    46441 tctaagttag gatgttatta atgggcaact tatcaggaaa cgttttctta tgtttttgtc
    46501 caattttttt gtgaggtgtt tattcttgaa aaaaataaag aaggagagtt atggcttcct
    46561 gacaatcttt gtcgcttgta taatgatggt tttacagcgt atattgattt cagcagtcac
    46621 tattcctaca tatgcaacaa accctggtcg attctacatc tatacatttt tacaaacttc
    46681 cgttgttatt ggtgcaaatt tgatcccact ttacatgggt taccaatatc aaaaaatcgc
    46741 ccgtttaaaa gttttacatt atttaagtcg ttttgccttt atattcctaa tagccacact
    46801 agtttgcaat atcttttttt acgtacaagc tggcagcttg aatattcgtg attattggct
    46861 tatcctaaca cctatcagtc agaattactt tacttatgct gtttcttgta ctttgctttt
    46921 ttgtagtatt ccttatacag ttggattttt aaataaatta tcgaaaacaa caattaaata
    46981 tctactatta tttttaacaa ttggattagt tggctgctct actctattta ataaagaccc
    47041 gtttgcatta cagaatggac agagtatttt atggattttt tatttatttt taatgggtta
    47101 tggtttgaaa gaatggaact ggaaagaaaa ggtccgctat aagcttcctc aagtatttat
    47161 cagcttgatt gttttattcg gtttgattat tttgatgaca caaatttcac tcatcatcag
    47221 aggaaataca gatacagcgc tgaggttttc taaaccatat tctctcttcg caatgtatta
    47281 tactttctct tcgtttttat ttttagaaat gctatctgat aaattaggta taaaaggaag
    47341 aggaaatttt cttgctgtat ttttgattgt cactcaagtt atatgtaatt tccctttagt
    47401 ttcttatcgc gtatctacat ttatgaaacg tgaatttcca aactcaggga aggcatggct
    47461 gataaatatc gggcagtttt ttatttgata tttattagca gtgtccctcc tagttatctt
    47521 tttattttat atacaaaaaa tccgtttcat tcatcgtttc atcgaatact tcgcttttga
    47581 ttcgattgaa caattaactc agcgttggaa aaaaagaatt cattggcttt ttgttcataa
    47641 aagttatttt ttagttgcag cattttttta ctgctttacc ttcgtgcaga ttttcgtttt
    47701 agaaccaaaa gaaggttgga aagaaactat ccaggttgcc ttaaaaatat ttactcagcg
    47761 acaagcaccc atgatcctga caatcatcat tattatggga tttttcttct tactattgtt
    47821 gatttccaat cgtttttggt acgccttgac tgctaccttg atcattaact tactgctcac
    47881 tatttcgaca gtaatcaaaa tggaaatgcg ggaagagcct gtctttcctt cagatttaaa
    47941 aatgctgacc ggattgtcag aactattatc gatggttagc ccagtcttat taatcgtggg
    48001 cggattgatc cttctcctat tattgattac tagtattatt gtacaacgta gactacaaaa
    48061 acagtattcc ttgaagattc attggaaacg tcgatttatc agtatcgctg tattattagg
    48121 atgtttttcg ggtgtctttt tcattaatca caaaaattcg ccttctttct tgctgtttaa
    48181 tttatttaaa gtaaacaaga ctttctttaa ccagcaaggc gccgtaaaag ataacggacc
    48241 agtcattcaa ttcctaaaca atatcgacat taaaatcatg gaagaacctg ctggctattc
    48301 gaaagaaaaa attaatggaa taatgaaaaa gtacaacaca aaggcagatg aaatcaacca
    48361 aacaagaagt gactggttaa acaatcagac gatcgttctc aatttaagtg aaagcttcag
    48421 tgatcctgca cgtgtcccta atctgacagt acctacagac cctattcctt acatcaaaag
    48481 cattataaca gaaggaacag gaggattgat gttatccgtc ggttatggtg gcggaacagc
    48541 taacatggaa tgggaaggac tgacaggctt gagtatcagt aacctgtctg cttcgttagt
    48601 tacaccttat acccagctag tggagcgtca gaaaataagt ccaaatatta ccgatctatt
    48661 cgatgaaaaa atagccatcc atccttttac agctgctctt tatcgcagaa aacaagtgtt
    48721 tgataaattt ggttttgaca ggttctatta tttagacagt ccggataaat taacttatac
    48781 ggataaaata gaacaaagcc cacgcatatc ggatgattca gcatacaaag aaaccttgaa
    48841 agctttacaa agcaatacgg aaacgtcaca attcatccaa ttatccacta tgcaaaacca
    48901 tatgccttat gatgattact atagtgaatt gaactacact gctgaaggaa ctgctgtcat
    48961 tcctaataga agaaaagaat tacaaactta tatgcaaggc cttcactata ccgatacagc
    49021 agtaaaaaaa ttcatccaag aaatcgacaa aatcgataaa ccgattacct ttgtttttta
    49081 tggtgaccat ttacccgcaa tctatagtgg gaatgattcg aaaaaatatg gattagaaca
    49141 gcaccagaca gattatttca tttataacaa caagtatagt cgtgaacaag cagctacgtt
    49201 aaataaagaa gttgttgctc ctaataactt tggtgctatg gctcttgagc atgcgaatat
    49261 caaagtaact ccttactatg cgttgttgac agaagtatct aatcatctac ctgcattaac
    49321 aattgatcct acagaaagct tgtctaatcg ttttaatgga aaacaggtct ttgttacaga
    49381 ggatagtcaa attatccaag aaaaaaattt aactgagaaa caaaaagaaa ttctagaaga
    49441 ttatcgtctg atacaatatg atcttgttgc aggtaaccag tacagtgcaa catgggcgac
    49501 acaaaaaata aaagactaac aaataaactg agcaaggggt tgtgataaaa gacatgtcac
    49561 aaccccttgt ttatctcatt aatttgcttg tccataaacg atttgattaa agccattttt
    49621 caattcatta ttcgctgtct cgttgaagta aagataatta tcgggattcc cctgattttg
    49681 ttgtgtcacc aatacttttg gctgattttg ctgaaacccg aatagataga cgtgcttttt
    49741 cagatatggt tctgtctctg catcggaata gacggctgtg atttgatagt ctgcttgacc
    49801 tgtcccactt tctgaccatt ctgcagaaac aggcactcca ccaatagcca tcttccactc
    49861 cccgttcaaa atagcttgag ggactttaag accatataag tccacactca tttgattcgt
    49921 ataacttttg tattgttgtc ccattgtttg gccccatttg gtcataaatg cctctaattg
    49981 tgcggatttg tctgcattcc acagtgtttg ttcaacttgt tcgctgcttt ctgaagagga
    50041 agatgaagcc tctacagaag aacttgaagt agatgtttga gttgtttcag tcgttttgga
    50101 agactctgac gaagtagagg tatctgctgt ttctgtactt gctgcagttg acttgctctc
    50161 tgtgctagtt gttgtagtag ttgcaactgt tgctttcgct gtttcttttg ttgattcttt
    50221 tttcgattca ttcccgttgg tacaaccagc taacaaaaaa gctagaccaa ttgttactaa
    50281 aatccttttt ttcaattaaa aaactccttt cgattactcc cacttcttta ttctatcaaa
    50341 taagcaatgt gattttattg atttttcgta ttttctcatc aatctttcaa atcttctaaa
    50401 aactgagaag cacccgctga tgttgtctgt aatacatcag cgggtgcttc ttttatttta
    50461 atcttcgtga ttttccaatt tacctcacgc tttatatttg tatttgaaga aacatagact
    50521 tttagtcatc atcgtcttcc tcatccaaat cttgtcggac agtttttcct tggagtcttt
    50581 gtacttggta gatgatttct ttattgaaac ttaaaagttc aacaagcaat tgttgcaagc
    50641 catatttttc ttctttttca gctaatttga ttgcccgtgt gataaagaca ttttggaaat
    50701 caaaatcttt tgcaaagttt tccaacattg ttgaggctgg taaatatttg ttttgaccat
    50761 gttcgtaaat ttttccataa cgcatgactt cttctaaagt agacggaacg atttcatttt
    50821 caatcactaa ctcatgagag attttattga accatctcgc ttcttgatca gcatattctg
    50881 aaaaaagggt acttaattct tgcgaagatg tacccaaaac atacaattgt gcttggatca
    50941 atttcatttc ttctaattta aaatttgcta atacatgccc caccatggcg ccagcagttg
    51001 gtttatgatg gtcgatatct gcttgttttg tttcttgaac aaatttttct tccactatat
    51061 tcattgattt acgcctcttt tacttttgct gattggactt cataacctaa tttttccaca
    51121 actgcagcta attgttcagg gtggacttca tctggatcaa agtttgcttt gattttgctc
    51181 gcattaaaca aaactttcgt attgcttacc ccaggctgtt gagaaaccgc atgttcgatt
    51241 ttttgcatac agcttggaca agctagtgta tctaattgca taataatttt tttcatcgta
    51301 gttcttcctt cctttttctt gatagtctta ttctagatta cagcttccat aaaaaaattg
    51361 attcttatca agttttacga tttttcttct ttgctttcgt gtccaaaata caataaacgc
    51421 atcgcgttaa agataacgat caagatacta gcttcgtgaa cgaacatccc actagccatg
    51481 taaataaacc cggcaaataa gccgttgagt aaaaataaga cgacaccaat ggcaatcact
    51541 acattttcac gggtatttaa aacagttttc ttagccaagc ggtaggcatg aaccaacgat
    51601 tcaaaattag attgcatcaa gacaacgtca ctcgtttcga tagcaacatc cgttcctgat
    51661 cccatagcaa tcccgatatc agccgcagcc aaagagggag aatcattgat tccatctcca
    51721 ataaaagcaa cgcgcaatcc ttgttcttgg tattttttca caaattgaac cttttcgtct
    51781 ggtaataatt cagcatggac ttcatcaatg cctaacatat cagctacata atcagcagtc
    51841 atctgattat cacctgtcag catcactaac tgttttgctc ctgcttgttt caacttagct
    51901 aattgttcag cgacttctgg tcgaatcaca tctgacacac ctatgatttg agtgattttt
    51961 ttgtcgattg ctacaataat gaccgagctg cctgtttgtt gtaattgatg taaatcttgt
    52021 acttgctttg gtgtgagaga aagtttgtga gcggcaatca atttggtgtt gcccgcacaa
    52081 actgtatgac catcaatctc agctgtgatt ccttgtccct tgattgtctg attatctttg
    52141 actgctaatt gttgaaaatc aatgtctttc tcctgagcat aatggatcac ggcgcgcccc
    52201 agaggatgat cggataacgt ttctaatcga gccactaaag ctaagctgtc cccttcatcc
    52261 tctgtatagt tcttgatagt ggatacagca gtagttcctt tggtcaatgt accagtttta
    52321 tcaaagacaa aagtatcgat tttggaaaaa gtatccattg cttcaccgcc tttgactaag
    52381 atgccccgtt tcgctccatt accgattcct gctacattcg aaacaggcgc accaatgact
    52441 aaagcgccag gacaaccaag aaccaaaaca gtgattgcta aacgaaaatc acgactaatc
    52501 agaccaacaa ccaacgcaat aagtaaaacg gttggagtat aatactgaga aaatcgatca
    52561 atgaattttt ccgcatggga tttggtatct tgtgcttctt ctactaattc gataattttg
    52621 gcaaaggtcg tatcatctcc gaccttagtt gcttcaatcg tcacgtagcc atctgctaaa
    52681 atcgttccag agaagacttc atctccactg attttgacga cttgtcgtga ttcaccagtc
    52741 actgcggctt catccgcata ccctttcccg tcaatgactc gtccgtccac aggaatagct
    52801 ccaccagttt ttactaagac gatatcgcct tcttccacgt catcgatttc cacttcttca
    52861 gtcgttccat cttcttggac aacactagca gtagatgggg ccatctcggt caaagccttg
    52921 attgactccc gcgttttggt caatgttttt tgttccagat agctaccaaa caaaaacaag
    52981 aatgtaacga tagctgactc cttgtattct ccgatcataa aggcaccaag cacagcaata
    53041 gtaacgagta aatcaattga gataactttg actttcactg cctgatacgc atggatcata
    53101 ataggtatga ccgcaataat agaagctatg gctagagcaa tgttatagaa caagacaaaa
    53161 ccagtaaatt ttccgataaa accaaaaata atcaacacag ctgataaggc ggtgatatga
    53221 tttcgatgct cttggatcca cttttgtaat ttcatctttc tccctctttt cctttcattc
    53281 ttacgagact aagtatagat acttatccat cataaaaaat tgaccactat caagtttagt
    53341 caaaaaggcg gttttctttt ggtagaatac actacaaaaa atagttagca agaagactca
    53401 ctaaaagtga tcttgccaac tattgtcgtt tctatcgaaa ttttcattga atgattttcc
    53461 tgtacagcat acatcggaga tctatttctt tatctaagat ttttatcttg ctaatttttt
    53521 cctttagtct ttcaaatcca cgtcgttcat agaattgata cgtacagcca ctatctgtaa
    53581 aaaggaatat ttcttttcct ttctctcttt tttcaaattc tgttagcaac ttggtaccga
    53641 tgccttttgt tttcaaatca ggatttgcag ctaaaaaaat gatctctcca tctggcgaat
    53701 gcttctttaa atatgctgca agcatttgtt catttgctgt ctcgtaagct cctttacttt
    53761 ctttagcaaa tatattttgt aaaaaatcga atatcttaac atatatttgc tgtccaaaag
    53821 aacgatgttt ctttttttct ccttttatct ccgccagcaa aacacctgcc aactcgtcct
    53881 ctgcatacaa agcaagaatt tgagttgctc gattcaactc catatacaaa aaatatcttc
    53941 cgtaaagatt cagcagaagt tcattttcca tatatctctc aaagttcatt ccttcaacag
    54001 caaaatagat agcttttttg taatctttct tttctaaggg tttaaaagtt agtttcatca
    54061 tttcgcctcc tcaacttcaa tgattgtgtt atacttacat tataatagac acagtgtcta
    54121 ctatctataa tcaatcatct ataagtttgt cgattaagcg acagctttgc tttgatatgt
    54181 cggataagga gataggtaaa tgaaaaagac agatagacgc gtcaaaaaaa cagaaaaagc
    54241 tttggctgaa acactatcca cattattagt aaataaaaag attcaagcaa tcactatacg
    54301 cgaactaaca gaaacggcag atgtacatcg ttcgactttt tatacacatt ataaggatat
    54361 ctatgatcta tatgatcaat tagaaagcaa cttttttcgg gatctaaatg aaattctctc
    54421 ttatgatcca gcccatagct atgaggaact atacactcgc ttgattgact atgtttacgc
    54481 aaatactttt ttatatcgct tatttatcct caaccatggt gatatttcgt ttcagaagaa
    54541 agttaaacaa gttattgaag aaaactatct gaacatatgg ctatatgaag acaagaaaac
    54601 gattatcaca gaagaaatga gattctttac tacgtaccac gtccaaggtt gtctatccgt
    54661 tctcaatcgg tgggtagaaa gcaattacaa gtactccaaa aaagaattat tagaactctt
    54721 acagaaactg aatgatcaca tagagaaaat catgccttga ataaaaacat actaagctta
    54781 gttaagaaga accgtaacct tctcgtataa caactgaatc ttatttagcc aaattgttta
    54841 ctgaagattt tttgagtaaa atttctataa ttcctactta ttgaaaagta gtttgtagtg
    54901 ctgcttctcc ctctctaagt gatatatctt gttgtaccaa tcaaattcac ctcatcatta
    54961 ataaattact tttaggctaa tttattacaa cttataggtt gaagctgtaa ccaatttata
    55021 gtcatattat atttttatca tccagtttat tgaattcttc tcgtttacct caattcattt
    55081 tcaaggggaa tcagctcatc atctaacatt ttacgccatg ctcgcttcat ttcttgtggg
    55141 tatttaaatg gtttcgcttc tggatgtttg gctttatatc cttcgtaatc cttactgttg
    55201 ataattgctt tcatattagg aattggatct tcacctggca tatattgcag attagaagca
    55261 tgtaattttt tctcagaaac aaaccactca ttggaaaaga catgaatcaa atcatcactt
    55321 acttgtgtaa aatagcgacg tttaatgaca aagatatctt cattgctatc tagtgtacct
    55381 tcatttcttt gtgcttaaat tcaaagataa ttttctcaat aaagattttt gattccagta
    55441 agattccttt actcctttta caacatagcg ttgtcctttg ttttgaaaca tcttcttccc
    55501 ctctttgcca ttaaaaatag cccaattctc tataaagaag attaagcttc attattataa
    55561 tataaagttt aaatttggtt tattacttac tttttcttat tctctagtaa gctaatattg
    55621 aaaaaaatgt aatcagatca ttatttttat atgttgtaac ttcctgataa gaaaactaat
    55681 taaaagaaag aggtaactac ataaactaat taaaaaagct aacttcatag ttggaggata
    55741 aatggaagaa acttcaatga ttgtttctcc ttgtgttcct tcaatcgaca ctagtcctat
    55801 tatcaatctc tcttttttgt ccatttactt ttgcgactgt tccccaataa tcaaatacag
    55861 gaagcgttat tttttgtcct ttagattcat gtactgtata gactattttc gaatccatca
    55921 cttgataagg catagtatgc cattccttgc caattcttac ttcatgattc tgcaaatgtt
    55981 ctttgtattg taatgcttcc gtgggtgcat agtcataaag tcctccggga aggagtgtcg
    56041 tgtaacgaat aagattttct tgattcatta ccaagtgatc tctctgagtg acaactttat
    56101 gttcatagtt tgcttttgtt gcagcagata cagccaacag taataaaaca attgccgtta
    56161 tattaactac ttttctttgc ttagctgatg tgctcttcat gaaaacgaac gataaaatca
    56221 acgagccaaa cagtacttga aagcctaaga ttcgccaagg aaattgaatc atttgaatgg
    56281 gtgtattttg taataaagac catggaaaaa atttggtgga taagagccaa gagactaccg
    56341 cactaaggta aatcactttt tctttgcgat taaatccttt aaaaaagaag agtggacaga
    56401 tcaaagaaat aataccaatg attcctaccg taaaggttgt caggtcatta ttcatcgcac
    56461 caatcaaact atccgagaca tttaatgctt ggtgttgtaa ggtcgtttta tctggctcat
    56521 taatcgattg aaccatgatt tgtcggaaca tcggatacca aaagtatgct gttaaaagaa
    56581 tcgtcattcc tactgccttt gacaactcca caatcaaccg gatgctttct ttttttcttg
    56641 tttcacttac tacaaagccg cctaagacta gtaagccaac ggctattaca ctcataaaag
    56701 tggaaagaac atgtgtataa atgagtaagc tcatactgac tgctaatgga attgcacatg
    56761 gctttttctc ataaatcagt gcgtaagcat aatacataac tatcggtata accgtaagtg
    56821 caatcgcttc agcaatagca gaacgatgat aaatatcaat cgtacggtac ccataaaatg
    56881 tatataaaca gctaaatata aaagccgcat cgattctttt taaaaatttt agtccataat
    56941 gaaacatcag catgatagta caaacattca atagccaaac atataagata taactaacga
    57001 ttaaattatt acttatccaa taaaaaatga ctgccggaaa aaaggtcaag tagggataaa
    57061 aataattcac tccgctccca taatgattaa acgtcgtaaa attgattgga ccagaaaaaa
    57121 ttgaagataa ccctttgact ctagcaaaat ggaaggcaat atcccccgat gtagaaatag
    57181 tatgaacatt tctaaaataa ttgattgaat aaagtccagc aataattgct aggagaatgg
    57241 acaatattat ttttttcttt acttgcatta cttaattata tactcctttc atggattgct
    57301 ttatctagta taacttaaat tttaaataaa tcgttattta tttttactct cacttaaaaa
    57361 agcactaaaa ttataagaga gggaggtaat ggtctgaaca ccaagaattg ttccaacatc
    57421 gaattgctta actaatgagt tcaaagctta attatgtggc tcttatattt accgagcgtg
    57481 gattgcgatc tctttagcga aacaaacatc tttcataaaa aaaacagcca tatccgcttt
    57541 cttggatatg actatttact actactttac tatctttcat tcttcttgtc tcacttatga
    57601 taaggcgctg ggtatcatga gtgaatgata ttgctgaata caaatagtcc gaccacatta
    57661 agttgatcgg acttcataag cattttattg actaatactt ttttgcttaa gaattgattc
    57721 taacatcttt tgctaaagtt aagcatccta tagttcctgc attatctgct aacttaggtg
    57781 tgacaatgta ttcttcgata tttggcgtat cgacatagcc gttaattagt tctaaaaaac
    57841 gtctttgtac tttctttttc agatgttctt gttgcatgac accaccacca aaaattataa
    57901 tatctggtga aagcatcagg gtcgtattac aggcacattg tgcaatataa tccgcctcga
    57961 tttcccagaa cggatcttct atagaaagat cttgtccctt ggttcccgta cgcatctcga
    58021 tagcgggtcc agatgccaac ccttcgagac aatcatgatg aaaaggacaa gtaccagaga
    58081 aagtatctcc tgaatgtctc ttaactaacg tatgccccat ttctggatgg ctaaaccctt
    58141 caacaaaaat cccatcttgt atagctcctc caccaattcc agttccaaca gtgtaataaa
    58201 caacactgga taaacctttt ccatgacctg aaacatactc cccgtaacaa gcagcattca
    58261 catcggttgt ccaggcaatt ggaatttcga aatgcttatt caacgttcca acaaaatcaa
    58321 aatttttcca ggcagttttt ggagtagagg tgatatgtcc ataggtcttt gattcacgat
    58381 ggatatcaat cggtccaaat gaccctactc caatgctttc taattgttct ttatagtgat
    58441 taaagaaatc aattaccaaa ctcattgttt cattcggtgt agttgtagga aaactcactc
    58501 tttctaaaac ctttaaatca tcattgccaa tggcacaaac aaatttagtc ccacctgctt
    58561 ctattgagcc atacatttct tactccaccg tccttttatc taggaattcg ttcttccctg
    58621 tctaatagtt tttcgaaaaa ctcatgtggt tccttttggt accaataggc aacagaagaa
    58681 atatcgtctt gacgctcata aagcttgatg tcatcatttc ctagggtttg gaaagtaaca
    58741 cgtaaatttt ctttgaaact aatggggtcg ggtaaatgcc agcggtacaa cccatgcatc
    58801 ggtaaagcat catcgccaaa accatgaact ggatttggat taggttttcc agcactaaaa
    58861 taatcccggg tgtgatctct tttagattga aaaggatagc ccataaatag cgtattaaaa
    58921 ttttgagcat atgccctttc tccaaagttc tttttatgga aagcccaagc gccgccaaaa
    58981 taatcttctg ctcctgtaga tgttattgta gggaaatctt catctccatc tatataaaac
    59041 ttaaattctc cttcccccca ccagtatctt tccaatgcgc acaaagcaaa gtaggttcct
    59101 acataatacc cagtgccttt gattccatca ataagggtgt aatcttttcc ttttgtcgtg
    59161 atgggttcac ggttccattt agcatgaaaa tacatcgcat catccggtac ttcagtcaat
    59221 gcataattaa atgtataaaa aatgtgctta ataaactcag gatgttcatt cgttaaagta
    59281 atcttagctt tttttcgaaa tggcatctca aaatagcaat tcatccctga tgttggattc
    59341 actacgattg gtaaggaatt cacttcgcat cttgtcccaa acccattaca aaaaaaatct
    59401 cctaatggac tttctacaga aggagtggat tcatcatccc aataaatacg caaaattaaa
    59461 tcacgtaaga cgaaactccc agcgattgtt ttgtctgtca ctgtcatcca aaaatgacgt
    59521 aaaattccag ggccttccat ttctgctaaa gtaattgtct ctccagcatt tatcggaaca
    59581 gaaggtgacc cttttctccc aattcctaga tgactcgctg tcattgccgc tttccctttt
    59641 tcaccatttt tattttctgg tgaaatagtt ctactttcct ctcccttgtg gagccatata
    59701 ttatttaaac aattcatttt attcgtcctc tatcctttct tcactttttt gattattcat
    59761 atagatgatt tgttgttgtg cctccgcaat aattgctcca atatctaaat tatcatttcc
    59821 taactgcaaa gcgattgaca ttgccaaggg aagattcatt cctgcaatta cgtgcactct
    59881 gtcagaaata taggtaaaaa acttttgatt tacacttcct ccagccatgt cagttaaaac
    59941 aattatttcg tctgtagtat ccactaactc tttaatttgt tcatctatat catcaatttc
    60001 ttcgacatat gcacataagc tactaatttg attatgcata cctgttaaaa actctagtgt
    60061 ctctttaaaa ccctcagcta gtattgaatg gctagcaatg atcaatcttc tcataattca
    60121 aatccttttc ctttacgcta atattccaaa tgcagccata accatcgcaa aaacaataac
    60181 gagtagaatc aaaatggtca tactgatttt tttcttactg agtagataat atgctgcagc
    60241 agtaataatt actggaatta atgttggtaa aattttgtcc aacattggtt ggattttcat
    60301 actaacttca ccaaaagtga atttaagtgg cgttgaaaca ctgataactg aagcaattaa
    60361 tgatccaact actgttagcc ccaatactga agcagcttca gttaaataat taatcttttc
    60421 accgtattca gtaacaatct tcattccctg cttataacca aattcaaagc ctctcattcg
    60481 gaaaaagaag attaccatga ttaaaatgaa ccataaaaag attcctacag gattattttc
    60541 taaagccata taagccgcaa ttgatcccat gatggttggg atcatcgtca tcaaaagaga
    60601 gtccccaata ccagcaattg gacccatcgt accaacttta aagtcattaa tcgcatcttt
    60661 tgcttctaat ccttgctttt cctccattgc aacacaagca cctaaaataa tggctgccag
    60721 ccaaggctgt gtattgtaat actgaaagtg attatttaaa gcttcgatgt attcatcatc
    60781 atctttgtat aattttctta aaataggtga caatgcgtaa gtcacagatg caccttgctg
    60841 tgtttgataa ttaaaggtac aaactgacat caaccaacga tagttcgctt tcctaagatc
    60901 ctttttcgtt acttgatatt tttcgctcat tttattcatc ctccccaata aacccaacat
    60961 cagaagctcc cgcagaagca gtgatttgtg catctttttc agctctatga tacacattaa
    61021 atgctaaagc gaatcctatg atcgccgctc caataatagg taccttcaaa aaagcggcta
    61081 agaaaaatcc aacaagtaaa taactaatgt atcgacctgt aggcataaag cgtaacaaca
    61141 tcgcaatacc aacagcaggc aacatttttc cggctaatgt tagcccatcc ataaaccaat
    61201 taggtaaaac atccaaaacg taattaacag cagactgtcc taaagtaacg gcaataaata
    61261 ctggaattgc tgcagataaa ccaaagaaaa ttggccccaa ccacaaaaca ctattcattt
    61321 tcttaaattc tcgtttattc gcatattcct gtgatttgcg aacaacaaaa ccatttaaga
    61381 ttttgacaat tacatctagt tgcaccccta acatacctac aggtagtcct attgctaatc
    61441 cggcttctac tccaagctgg ctaccaaatg ccgttgcaat aattgcgcac gttccatagt
    61501 ccggtacaga tgagccacca aaaccggcaa ctcctaatga catcaattga actgtaccgc
    61561 caactatcaa acctgttttt atatcaccca tcaaaattcc tgtgaataat ccccaaaaaa
    61621 ctgcgtaatt tacaaagatc atcggaatcc cataataatc aacatgttta ataaatgcta
    61681 atatagtcag cataataatt tgaaaaatag taaaatgcat tatccttact cctttatcct
    61741 aattttgatt gaatttcctg tgccttatcc gccggaacaa attgtgtaac aatttctaca
    61801 ccagaatcca acaatttttt atacgtgctg atattttcat ctgatgcata ggctctagaa
    61861 gatagctgta atgcattatc tttaacaaca gtaccgccaa caattagtcg gtcaattcta
    61921 ataccgttat ctactaaatg aaggattgtt tccggctctt tcacgatgac aaatactttt
    61981 tcctgatcgt atttcccctt tttaaaattc gcaatcgctg tttcttcagt gataattgat
    62041 acagccattc cagcaggtct agctaaacgc atacttgatt tttggatgtc gttatttgcg
    62101 acagaatcat cgatcaccat cactctgttt gctttagaca ttggtgccca ttgcgtgaca
    62161 ataatcccat gcaataaacg gttatcaact ctcgacataa caatactcat aaatacttcc
    62221 tcctttatct ttcattttct tacaagcaat taccatgcca aaacaaaaag aacttgataa
    62281 atcaagctct ttttacataa tcataaacgt atttaacctc atgaatcggg atgtcgacac
    62341 tataataatg ttcaacgaca ctgaaagatt gcttaaaatg tgtctgaaat ttttgaaatt
    62401 cctcactcgt ttcattgaaa taaatttcat cttccaaagt attttgtttt actaatcgtt
    62461 caattaagca actaatgtga acatataatc cgaaacacgt attattatca aatgaaattc
    62521 ctagatcatt ttgtaagatg tatatagcat ctgaaacccg ttctaataat ttagtcggat
    62581 ttaatatggt caattgctcc attaaattcg ttagactaaa attcttcaga atattattgt
    62641 tcaaaacttt cagttcatta tcattaaagt gtctattcaa ttgattggag aatttactta
    62701 tattcattcc taaaatcata tcttcaattg aaaagaattc tacatcttct agatgtggat
    62761 ttagtgttcc aacaataaaa agaatattgt tctctttacg aagctccgat aaatagtttt
    62821 cattcactaa agagtaatag tcacacgttt ctatgacaag atcagtatcc cttggaaagc
    62881 tatcttcaag tatccttttt aatttaaacg aagttcctaa tccgcttgca caagaacaga
    62941 caatcatatc cttcttaata tttgatgatt cataaatata atccacattt tgattttctt
    63001 ttacttgttc aagaatctgt ttaagcggta aatctaataa tatctgattt gcaacatcaa
    63061 tagccattct tgttgaaaca ttgtttagta gtccgaattt tttttgagcg tctagttcca
    63121 aatttagata aattttctcc agtgaaccca tatcaacaag taaaataatt tcttcatatc
    63181 cggtacactc ttgtaagtat ctattaattt tgtctgcaat ctcactactt gaagtcatta
    63241 gtggcatatc aatggcgtcg tatatatatc tctcaacgat cctattagca gtacttgcta
    63301 tacaggttgc cgttagatct ccatgagcca aaatcagtgc taaacgtttt tgctgaaatt
    63361 ctttactaca cattaactta aataaaataa ccaaaacatt tttttgaatt tttgttaaat
    63421 aaatatcccc tatttgaata aaacgattga ctaattgaac ttctcgacta caccaactat
    63481 taatatcatt gatgaaggaa tataatttat cgtccgatgt taattggatt tgataaatcc
    63541 aagaattcaa aaagatagac gttatttgaa gataataata gtcaaattct atttgaaaat
    63601 ctctctctat ttccttaaca tactgtctaa atcgaatctc ttgataggtg ggatatgtta
    63661 aagctgaatt attttggata tcaacattct tttgtctaaa atatctatca tatttctcaa
    63721 aaagctgctg cttatcagtg tctttcttcc acgtttgata aagatactct ataagttcgt
    63781 tgatattaaa aacttcgtct actttttcca aatcatccat ttgaatatat tttaattcat
    63841 tctgtctttt cataaccttc atgccgttga aattcaccca ttgtggtaaa tgatacatct
    63901 gaatataaag ttcttccaaa tcatctaaga aataaaaggc attggcacag gtcaattgaa
    63961 tgatattttt caaattgcca acattttcac taaagcgata gtttaataaa aaatctaata
    64021 attgttgaga tatcagtatt ttctttgcta gtctttcttc ttctgattta aaaaatatct
    64081 tgattaactg ttttctttca taaagagaac gactgtctaa tgttggaagt gttagcttta
    64141 caggtattct tctcaacaaa gttgataata gtttatcctc aggtggttct gatgttgcaa
    64201 atataatcca gcaacttgat gtgtaccagt tttcattatc acccatccga tgaaatttcc
    64261 tcgtatccat aaactgaaaa agtttctcct gacattcatt gctcaatgca tgaatttcat
    64321 ctaaaaataa gactccatta ttagctaatt ctgctaaacc ctttcttcca tcttcggctc
    64381 ctgtatatgc accttttaca tgcccaaaaa aattggacaa gaaaagttct ggattattgg
    64441 catattcact acaattaact gaaatgaaag atgccttctc ttctatataa ttattggcca
    64501 acaaatcctt atacacataa tttgctaaat agctttttcc agttccagtc tcgccatgaa
    64561 gtaaaatggg taagccattt ggcggatagt agattgctgc tttaatttga gcaattacat
    64621 ttcgcagact tccttgactt ccgataaccc tttctattcc aaccctaaaa gagctatcat
    64681 accatttttc cgcttcctct gaagagctaa aaatttgttg atttggaact ttctccggta
    64741 aagaaaagaa gattactgga cgagtattga tttttccaaa ttctccatct tttacgaatt
    64801 cattcaaata ttgagagaca atattacgac taacatttaa tttttcagat attttctgtg
    64861 ctgtattgtt cccccattta tttttaatct cagagttctc tatgacataa ttgacaatgc
    64921 gttcttttaa ttcttccatc acagcacctc ctcgacacta ttcgaatttt gccgacagta
    64981 tttttccata aatatgactt gctaaaaagg ctttgtttag atcattcaat tggaacgttg
    65041 cttgttttgc ttgatccgct aagacgttga aaagatactc tggcgaaatc gcatacccca
    65101 aaatatcaac gattgttgca atcagatctt cttttgattc ttcatctgaa agtaattcac
    65161 gatacaacat cgtttgtttg cttactgtgt catattcctc taaggattca tctgctaaaa
    65221 gaaccacttg ttccaacaaa cggtcggata aatatttata gaaaatcagc cccaataaat
    65281 aatttttata ttctgacgca tccattttac tacggagatt atctgccgca ctaaataact
    65341 tctgatttaa ttctgcaccc aaaaagatac actcctatct cttatttaaa gttgctatat
    65401 accctcttat tttagcatac tcagttttag aaaaagcaaa aaaataaaga aacttattct
    65461 tgataacaag ctacgtgacg tatatcccta acacaaaaat agccatatcc tttggtttgg
    65521 atacggctat ttgatattac tcattcaatt atcaaatcac agtaggtgtg aatctcatcg
    65581 ggatcggcaa tatctacttc tgattaggat caaaggtgat tttctcaata aaggtgtgat
    65641 acaaagcttt aacttgcttc ttctcactct ggataagaaa gcaatccaaa ctagtcaata
    65701 tctgttggac atctttgact tgaatcggtt ccccttgttg ttgaaagatc cctaagattt
    65761 cattctctcg aatttgtaac tgtcttcgtt tttcagtcaa ttcatttaaa cgatctttta
    65821 acatcggaaa caattcggga ctatcttcca aagcagcttc ctgtttttgc tctaatagtt
    65881 agattttgcg taccattttc ttcttttatt ttccttcgat tgctgatttt aatatagtct
    65941 aaaggaaatt tatatttatt gtattcttta taacttcaag agaacttcgt ttgagtatta
    66001 ctgtattttt ttaattccat aagaaaatta gttattacct gccacctaat cagcaaatct
    66061 gaacctgagt caatataatt aatattattt tccatctcca acattgtaag tactgttggt
    66121 gttaatatag ggactttgta caattgtatt tttttgattt tatcatctgc acaaaggaga
    66181 ttttttaaat ctaatccttc ttttatttca aaagttgaat tttttaaatc tataagagta
    66241 aaaccaattc cttcatactg aatttctgaa aatcgaattt ttctcccagt tgatagatca
    66301 aggggcagaa aaccttttat tacattgtga gtgtcaccat catttacaat gtcattagga
    66361 attttgattt tagaattctg tggagcacct attggaagat aataattcat tataattttg
    66421 tagtcttcat gaggaaataa agtactttta ggattgctaa gatattctcg taccgaatct
    66481 aagaatggct tatccattga atcttcatcc ccatttaaac taaaaaacat tcctacaatt
    66541 gcttttgcaa ctctaagtga attcattttc tttattgaat gtgttaaatc agacttacca
    66601 ttctttaaac ttgtattgtt ataaaggaaa tttttattgt cttctgcaaa tctaatatat
    66661 tcctcaacat actcactacc aaaaaaacta ttacatttac tacataacgt attggaatgg
    66721 caacagtttt tttgacaaat tttataaggt gcagaacttc tttccgtatg ctattccgat
    66781 tgtccttgac aatgagcctc ctcggatgtg acgatcttcg gcaggagcta acagttaaag
    66841 ttagactgcc tcgctagcgt tagcacatcc gagctcatta tcaaggattc gctgcgccaa
    66901 t
//
LOCUS       DACCYH010000005        61702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.005, whole genome shotgun sequence.
ACCESSION   DACCYH010000005 DACCYH010000000
VERSION     DACCYH010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.005"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(122..499)
                     /locus_tag="IVY85_000302"
     CDS             complement(122..499)
                     /locus_tag="IVY85_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043343.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            755..1483
                     /locus_tag="IVY85_000303"
     CDS             755..1483
                     /locus_tag="IVY85_000303"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP8043344.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1483..1737
                     /gene="purS"
                     /locus_tag="IVY85_000304"
     CDS             1483..1737
                     /gene="purS"
                     /locus_tag="IVY85_000304"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP8043345.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1739..2410
                     /gene="purQ"
                     /locus_tag="IVY85_000305"
     CDS             1739..2410
                     /gene="purQ"
                     /locus_tag="IVY85_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP8043346.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2411..4633
                     /gene="purL"
                     /locus_tag="IVY85_000306"
     CDS             2411..4633
                     /gene="purL"
                     /locus_tag="IVY85_000306"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP8043347.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4618..6057
                     /locus_tag="IVY85_000307"
     CDS             4618..6057
                     /locus_tag="IVY85_000307"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP8043348.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6089..7132
                     /locus_tag="IVY85_000308"
     CDS             6089..7132
                     /locus_tag="IVY85_000308"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP8043349.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7129..7707
                     /gene="purN"
                     /locus_tag="IVY85_000309"
     CDS             7129..7707
                     /gene="purN"
                     /locus_tag="IVY85_000309"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP8043350.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7704..9239
                     /gene="purH"
                     /locus_tag="IVY85_000310"
     CDS             7704..9239
                     /gene="purH"
                     /locus_tag="IVY85_000310"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP8043351.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9255..10529
                     /gene="purD"
                     /locus_tag="IVY85_000311"
     CDS             9255..10529
                     /gene="purD"
                     /locus_tag="IVY85_000311"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP8043352.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10651..11565
                     /locus_tag="IVY85_000312"
     CDS             10651..11565
                     /locus_tag="IVY85_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP8043353.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11698..13263)
                     /locus_tag="IVY85_000313"
     CDS             complement(11698..13263)
                     /locus_tag="IVY85_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8043354.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13256..14707)
                     /locus_tag="IVY85_000314"
     CDS             complement(13256..14707)
                     /locus_tag="IVY85_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8043355.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14721..15737)
                     /locus_tag="IVY85_000315"
     CDS             complement(14721..15737)
                     /locus_tag="IVY85_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8043356.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15957..17045
                     /locus_tag="IVY85_000316"
     CDS             15957..17045
                     /locus_tag="IVY85_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8043357.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17099..18625
                     /locus_tag="IVY85_000317"
     CDS             17099..18625
                     /locus_tag="IVY85_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP8043358.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18627..19832
                     /locus_tag="IVY85_000318"
     CDS             18627..19832
                     /locus_tag="IVY85_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8043359.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20089..21066
                     /gene="guaC"
                     /locus_tag="IVY85_000319"
     CDS             20089..21066
                     /gene="guaC"
                     /locus_tag="IVY85_000319"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP8043360.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21330..22754
                     /locus_tag="IVY85_000320"
     CDS             21330..22754
                     /locus_tag="IVY85_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8043361.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23215..24324
                     /gene="pdhA"
                     /locus_tag="IVY85_000321"
     CDS             23215..24324
                     /gene="pdhA"
                     /locus_tag="IVY85_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP8043362.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24327..25304
                     /locus_tag="IVY85_000322"
     CDS             24327..25304
                     /locus_tag="IVY85_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP8043363.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25329..26972
                     /locus_tag="IVY85_000323"
     CDS             25329..26972
                     /locus_tag="IVY85_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP8043364.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26980..28386
                     /gene="lpdA"
                     /locus_tag="IVY85_000324"
     CDS             26980..28386
                     /gene="lpdA"
                     /locus_tag="IVY85_000324"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP8043365.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28548..28823
                     /locus_tag="IVY85_000325"
     CDS             28548..28823
                     /locus_tag="IVY85_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP8043366.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28829..29596
                     /locus_tag="IVY85_000326"
     CDS             28829..29596
                     /locus_tag="IVY85_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP8043367.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29764..31593
                     /gene="typA"
                     /locus_tag="IVY85_000327"
     CDS             29764..31593
                     /gene="typA"
                     /locus_tag="IVY85_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP8043368.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31854..33626
                     /gene="recQ"
                     /locus_tag="IVY85_000328"
     CDS             31854..33626
                     /gene="recQ"
                     /locus_tag="IVY85_000328"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP8043369.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33740..34042
                     /locus_tag="IVY85_000329"
     CDS             33740..34042
                     /locus_tag="IVY85_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP8043370.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34045..35208
                     /locus_tag="IVY85_000330"
     CDS             34045..35208
                     /locus_tag="IVY85_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8043371.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35234..38662
                     /locus_tag="IVY85_000331"
     CDS             35234..38662
                     /locus_tag="IVY85_000331"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP8043372.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38683..39807
                     /locus_tag="IVY85_000332"
     CDS             38683..39807
                     /locus_tag="IVY85_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043373.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39797..40234
                     /locus_tag="IVY85_000333"
     CDS             39797..40234
                     /locus_tag="IVY85_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8043374.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40218..40556
                     /locus_tag="IVY85_000334"
     CDS             40218..40556
                     /locus_tag="IVY85_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP8043375.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40675..41235
                     /gene="rsmD"
                     /locus_tag="IVY85_000335"
     CDS             40675..41235
                     /gene="rsmD"
                     /locus_tag="IVY85_000335"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP8043376.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41232..41723
                     /gene="coaD"
                     /locus_tag="IVY85_000336"
     CDS             41232..41723
                     /gene="coaD"
                     /locus_tag="IVY85_000336"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP8043377.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41713..42756
                     /locus_tag="IVY85_000337"
     CDS             41713..42756
                     /locus_tag="IVY85_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8043378.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42915..43643
                     /locus_tag="IVY85_000338"
     CDS             42915..43643
                     /locus_tag="IVY85_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP8043379.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43759..44004
                     /locus_tag="IVY85_000339"
     CDS             43759..44004
                     /locus_tag="IVY85_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8043380.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            44001..44219
                     /locus_tag="IVY85_000340"
     CDS             44001..44219
                     /locus_tag="IVY85_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP8043381.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            44355..44894
                     /locus_tag="IVY85_000341"
     CDS             44355..44894
                     /locus_tag="IVY85_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP8043382.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44887..47160
                     /locus_tag="IVY85_000342"
     CDS             44887..47160
                     /locus_tag="IVY85_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP8043383.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            47198..48244
                     /gene="holA"
                     /locus_tag="IVY85_000343"
     CDS             47198..48244
                     /gene="holA"
                     /locus_tag="IVY85_000343"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP8043384.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(48333..48587)
                     /locus_tag="IVY85_000344"
     CDS             complement(48333..48587)
                     /locus_tag="IVY85_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP8043385.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48987..49256)
                     /locus_tag="IVY85_000345"
     CDS             complement(48987..49256)
                     /locus_tag="IVY85_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043386.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49726..50181)
                     /locus_tag="IVY85_000346"
     CDS             complement(49726..50181)
                     /locus_tag="IVY85_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP8043387.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(50319..52247)
                     /locus_tag="IVY85_000347"
     CDS             complement(50319..52247)
                     /locus_tag="IVY85_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8043388.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(52444..53277)
                     /locus_tag="IVY85_000348"
     CDS             complement(52444..53277)
                     /locus_tag="IVY85_000348"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP8043389.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(53469..54173)
                     /locus_tag="IVY85_000349"
     CDS             complement(53469..54173)
                     /locus_tag="IVY85_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8043390.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(54196..54822)
                     /locus_tag="IVY85_000350"
     CDS             complement(54196..54822)
                     /locus_tag="IVY85_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8043391.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            55148..57523
                     /locus_tag="IVY85_000351"
     CDS             55148..57523
                     /locus_tag="IVY85_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP8043392.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(57547..58038)
                     /locus_tag="IVY85_000352"
     CDS             complement(57547..58038)
                     /locus_tag="IVY85_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP8043393.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            58225..59145
                     /locus_tag="IVY85_000353"
     CDS             58225..59145
                     /locus_tag="IVY85_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8043394.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            59299..59643
                     /locus_tag="IVY85_000354"
     CDS             59299..59643
                     /locus_tag="IVY85_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP8043395.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(59833..60333)
                     /locus_tag="IVY85_000355"
     CDS             complement(59833..60333)
                     /locus_tag="IVY85_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8043396.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            60861..60944
                     /locus_tag="IVY85_000356"
     tRNA            60861..60944
                     /locus_tag="IVY85_000356"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60895..60897,aa:Leu,seq:cag)
     gene            complement(61268..>61702)
                     /locus_tag="IVY85_000357"
     CDS             complement(61268..>61702)
                     /locus_tag="IVY85_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP8043397.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        20233 a        10662 c        13216 g        17591 t
ORIGIN      
        1 gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc aataatagga
       61 acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga
      121 attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca
      181 agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat
      241 gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg
      301 tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt
      361 ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca
      421 aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt
      481 gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtt
      541 aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta taagattttc
      601 gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca tatttttgtt
      661 gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa aacgatgtat
      721 ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac ttttgtatga
      781 aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga tagaatattt
      841 agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa aaggagaatt
      901 gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa taaaaaatca
      961 ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa ttatccctct
     1021 ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag gtgttcccga
     1081 aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc agttggatga
     1141 tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg aagaaatcag
     1201 tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat ttgctaaggt
     1261 cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac aaattttgct
     1321 tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga atgaacatat
     1381 ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc aagaagtata
     1441 taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt cgtaaaagta
     1501 tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa aaaagcagtc
     1561 catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt tgagatacaa
     1621 cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga caagctgctt
     1681 gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga agtataacat
     1741 gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt tatgggcagt
     1801 tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct ctttagaagg
     1861 atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga gatgcggtgc
     1921 gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag aagggaaacc
     1981 agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt tgccaggtgt
     2041 actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga aagtcaaccc
     2101 caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc cagttgctca
     2161 tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa aaaataatca
     2221 gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg cagggattac
     2281 gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag tagaagactt
     2341 gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact tcggaaaggt
     2401 gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga aaaaaagaat
     2461 ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag atatcttagg
     2521 aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg aacattgttc
     2581 ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc atgtgttgca
     2641 aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag ttgtcttcaa
     2701 agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg cgacaggagt
     2761 tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt tgctagattc
     2821 attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag aagtagttgc
     2881 aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg aaattgcatt
     2941 tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt tgattgaaca
     3001 caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt atgtaggtgc
     3061 aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat ttaatcaaga
     3121 agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa agctgctgct
     3181 tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta tacaggatat
     3241 gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg gctcaggact
     3301 tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt atgaaatgat
     3361 gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg aagaaaaaat
     3421 ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag taacagacga
     3481 cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag tcgatgcatt
     3541 agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa tcgtagaatt
     3601 tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat tgcttgatct
     3661 gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg attcacaagt
     3721 acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg ttagagggac
     3781 gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt tggatccaaa
     3841 aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta gcggtggtaa
     3901 gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag aagttttttg
     3961 ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag gaactcctgt
     4021 tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct atccaacacc
     4081 aatgattggt atggtcggat tgattgagga tcataaacat atcactactc aggaattcaa
     4141 aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg atggcagtga
     4201 attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg atctatccat
     4261 tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga ttgaaagtgc
     4321 tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt tcaaacatca
     4381 gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag aaactcaatc
     4441 aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa tgatgggaga
     4501 tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt cagcaactga
     4561 cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg caatcgtatg
     4621 tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg gggtcatcca
     4681 gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg acaagaaggt
     4741 gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt ggggctgctt
     4801 tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc tgcaatcgga
     4861 catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc ttttctattc
     4921 aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa tgctcgttcc
     4981 cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga tacggagata
     5041 ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt aaaaagtgct
     5101 ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc aatgattgcc
     5161 gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa cggagcatac
     5221 gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat aagagatgtc
     5281 cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg ctttacgaag
     5341 gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc agattcaaac
     5401 atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt agcgaaagaa
     5461 tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc agccgcaagc
     5521 ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa tcaatacatt
     5581 gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg aatgaaatta
     5641 tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga ttcaattgtt
     5701 cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc aaaagaagtc
     5761 catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat tgatattcaa
     5821 acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga atgtatcggt
     5881 gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg tctggaaaaa
     5941 gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc gacgccgtta
     6001 tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt cttttaaaag
     6061 cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag ctggcgtaga
     6121 tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa aaacggaacg
     6181 tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat cggtctttca
     6241 attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat tagcagtggc
     6301 aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt gtgtcaatga
     6361 tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa cggggaaaaa
     6421 tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg ttcaaggaaa
     6481 tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag gtaatgatta
     6541 tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga caggagaaac
     6601 gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt caaatgggta
     6661 ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat caaaactgcc
     6721 agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct atgtttcttc
     6781 cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca ctggtggagg
     6841 gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa tcaaacttgg
     6901 ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa ttcctgagat
     6961 ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc aagaatcgaa
     7021 agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg aaatcgggaa
     7081 agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat gagaatcgct
     7141 gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct atcgaaaaaa
     7201 ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata tgttttgaaa
     7261 cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt tgattcaaaa
     7321 aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga tttgatcgta
     7381 ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga taaaagaatc
     7441 atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat acgtgatgcc
     7501 tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca aggtgtagat
     7561 actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac gttcgattct
     7621 ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat tagtgaaatt
     7681 attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca gataaaactg
     7741 ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt tcaacaggag
     7801 gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa gaagtgacag
     7861 ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc catggtggtc
     7921 ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa aatatccaac
     7981 caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg aaaccagatg
     8041 tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg ctccgtagtg
     8101 cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat gaagaagtac
     8161 tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa ctagcggcaa
     8221 aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta acggattggg
     8281 tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa acgcttcgtt
     8341 acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg gaaaaatatt
     8401 cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat atccgagatg
     8461 cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc gccttgaaac
     8521 acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg gaagcagcgt
     8581 atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga gaagtagatc
     8641 tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc ccttcttata
     8701 cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg acagtagact
     8761 ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt ggacttcttg
     8821 tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg acaaaacgta
     8881 agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc aaacatgtga
     8941 aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga gccgggcaga
     9001 tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa atggaaggtg
     9061 cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat gcagccaaac
     9121 atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat tctattgata
     9181 tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt agacattaga
     9241 gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa catgcaataa
     9301 gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca ggaaatccgg
     9361 ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat tcagcgttaa
     9421 tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa gttcctttgt
     9481 taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt ccgacaaaag
     9541 cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat acttatcaaa
     9601 tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg tatatcaaag
     9661 aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa ggtgtagtcg
     9721 tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac cagcatcgat
     9781 ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa gaattctccc
     9841 ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa gatcataagc
     9901 gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat tcaccagtac
     9961 cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa ccagcggctt
    10021 tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt ttgattgcta
    10081 cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct gaaacgcaag
    10141 tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta ttagagaatc
    10201 gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg gttgcagctg
    10261 ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg gaagaagagc
    10321 tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga gcaggaggac
    10381 gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa aaaatttaca
    10441 ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt ggagaaaaag
    10501 ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa aaaatacaac
    10561 aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt ggtacacttt
    10621 tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg tacttctgat
    10681 cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga agatccagta
    10741 aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc agatatttat
    10801 ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc aatcaaacga
    10861 gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt tgatttttct
    10921 aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat ggattatgtg
    10981 gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt agctgaagcg
    11041 tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa tcaaaaacca
    11101 atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa tcagctgcaa
    11161 tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat gacagatgaa
    11221 gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga tatgacgatc
    11281 caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg gaacgaaaaa
    11341 ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac gacaccaact
    11401 ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat cgccggtacg
    11461 atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat gtctcgggaa
    11521 gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt gtcataagca
    11581 ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt cagcttcgag
    11641 atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt tcgtttatta
    11701 gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa tcccttgttt
    11761 tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa aagcaatatc
    11821 tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc tttgttctgt
    11881 cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga aatgagaggg
    11941 acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg taaaattttc
    12001 tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg aaggctgtga
    12061 gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc
    12121 ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt gcgggatttt
    12181 cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac gatataatcg
    12241 tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag atacttcgtt
    12301 tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat caactggaag
    12361 tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg aactggcggt
    12421 ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac
    12481 aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat ggaccaacgt
    12541 ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa ctattaaaaa
    12601 tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc taaataaaaa
    12661 cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc
    12721 taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag caagtttcaa
    12781 tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat tttttttcaa
    12841 ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac
    12901 tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca gataatcatc
    12961 tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac taataaatat
    13021 gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa
    13081 aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg aagacagtgc
    13141 catctcacca tctccagctt cgccgacata agtgattgga taataagaac caaggacagt
    13201 tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa aaactttata
    13261 catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg acagttgttc
    13321 cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa taaagaagga
    13381 gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg atccctttgg
    13441 ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg ttgtctataa
    13501 ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat cctttgtgac
    13561 ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag atcattttta
    13621 aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc atatggccgt
    13681 atcttatttt taagatgact agataatcat tgatatggct aatttctttt tcaagaggaa
    13741 tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag
    13801 tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc aaactgttat
    13861 aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt gctaaatggt
    13921 attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt ttattcatgc
    13981 tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag ccaaggttcc
    14041 ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg acctttctct
    14101 ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact ccaagttcca
    14161 ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga
    14221 tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca gaatctacct
    14281 gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta ttgatcagct
    14341 cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac gtactaattt
    14401 ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt tcttctagga
    14461 tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc cacatacttt
    14521 ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg
    14581 tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc aaagggataa
    14641 cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt
    14701 ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat attctgctgg
    14761 gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt ttgaatattt
    14821 ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt acactcgact
    14881 acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt
    14941 acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc taaagcaccg
    15001 tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc atttgtttct
    15061 aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac aattcggtga
    15121 acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt tggtttacga
    15181 ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca
    15241 gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat
    15301 aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg
    15361 ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc tcgctttgct
    15421 tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat catatccact
    15481 ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat gtagccattt
    15541 cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc agtatgcttt
    15601 ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct
    15661 tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa
    15721 aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta tcttatcaga
    15781 taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa
    15841 atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg
    15901 ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga taaaaaatga
    15961 aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc attctattaa
    16021 gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga atcgctatgc
    16081 cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg gaattagaaa
    16141 aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat caagtagcac
    16201 aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct atcgatggtt
    16261 cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg
    16321 atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat aattttggag
    16381 taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa ggaaaaggac
    16441 cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta atcaattaca
    16501 acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc gtgccatctg
    16561 gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg gcgcaagcaa
    16621 ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct gttttgtcgc
    16681 catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg
    16741 aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc gttaaatcga
    16801 tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta gcaaaaaata
    16861 cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat gatactgaaa
    16921 cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta tctgtcgata
    16981 aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc gatctgaaaa
    17041 attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga taagaattat
    17101 ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa tccgagcgtt
    17161 agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg
    17221 agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg gctcctatga
    17281 cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga
    17341 aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt caataaaaga
    17401 gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg aactgacaga
    17461 aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga atcttctagt
    17521 atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat tatcaaaatc
    17581 cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag aaagcgcgaa
    17641 tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca ttatttcaca
    17701 taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg atggacagac
    17761 gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg gaatggtggg
    17821 cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag tttattttaa
    17881 agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa acgaccacat
    17941 caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg gagcgggcag
    18001 aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat ccggacgtat
    18061 attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg caaacggact
    18121 cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg atattcggga
    18181 aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag ataaagaaaa
    18241 agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa actccatcca
    18301 tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct
    18361 gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag atgttggggc
    18421 aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag ctgtctgtct
    18481 tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata cgatgaacga
    18541 aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct tgatgaaatt
    18601 aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg gataaaaaag
    18661 agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac agcatggtga
    18721 tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt ttctggcgtc
    18781 cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta gctgcaggga
    18841 tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg
    18901 ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg atgacagtta
    18961 tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg gtagcttatg
    19021 cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt ggactgacac
    19081 aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa aaaatgtcta
    19141 cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca ttgattttag
    19201 gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa
    19261 atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta tttacagcag
    19321 tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg attattttga
    19381 tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca gtagcaggtc
    19441 gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg atcaaaacaa
    19501 agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta gctggattag
    19561 ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg gaactggatg
    19621 caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga acaatcatgg
    19681 gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg attttaggcg
    19741 tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc gttgtgctag
    19801 acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca gaaaaaagac
    19861 tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca ttgtttcgtt
    19921 tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc tttttgctct
    19981 tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc ttgattaaat
    20041 taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat gaaagtattt
    20101 gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg ttctgaatgt
    20161 gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc tgccaacatg
    20221 cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta tttctatatc
    20281 atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga
    20341 ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat agagaagttg
    20401 gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg acatgctaac
    20461 tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc
    20521 ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc agatgcaaca
    20581 aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg ctttgggact
    20641 ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc
    20701 gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt tggtgcgaca
    20761 atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga aacaaaagtc
    20821 gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca aaaaggcgaa
    20881 aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt gaaagataca
    20941 ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg aagagatctg
    21001 gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca
    21061 atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca ttaagtaata
    21121 agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt attatcgcag
    21181 atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt tttgtcacag
    21241 tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta aaatcaaaag
    21301 aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa aaacaaggac
    21361 cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga aggatcaatc
    21421 ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg aatcgttcca
    21481 ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc aaacgtggca
    21541 gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga atcgattggc
    21601 ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa ataagagaag
    21661 caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg ccagatgagc
    21721 ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg gaagaaaaag
    21781 aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat ttggaaagta
    21841 aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt cagcaagcaa
    21901 tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta gagtcacctt
    21961 ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt gggataccga
    22021 tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa aataaattga
    22081 aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg agtcaaaaac
    22141 gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt gaagacgatg
    22201 tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga acgattgatc
    22261 cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca acgaaaatcg
    22321 gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa gaaatgatgg
    22381 agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat gaagcaattg
    22441 atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg ataagcgctt
    22501 ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac tatgcctgga
    22561 ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta gatatgggtc
    22621 ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga atcaattaca
    22681 gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt ctagaaaagt
    22741 ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct tggatttaaa
    22801 tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact aagaaaaaac
    22861 cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa caaaactgta
    22921 taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa aatagaaaaa
    22981 atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat gctgttaata
    23041 tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt gtatttctca
    23101 taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt tattcactgt
    23161 ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg acaaatggca
    23221 aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc agatttccca
    23281 gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt acctgattta
    23341 tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt attagaccaa
    23401 cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac tgctggacaa
    23461 gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct tcttccaggt
    23521 tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc ttttttatgg
    23581 tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct accaccacaa
    23641 attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt gaagaaacga
    23701 aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca aggtgatttt
    23761 tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta tatccaaaac
    23821 aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac acttgcacaa
    23881 aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc gcttgcagta
    23941 tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc agtcctaatc
    24001 gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc aacccgctac
    24061 cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg cttccgtaaa
    24121 tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat cgaagcaact
    24181 aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca aaaggtttca
    24241 gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca aattgcaatt
    24301 tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa gcaatcacag
    24361 atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt gaagatgttg
    24421 ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc ggagaagacc
    24481 gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc ggtcttgcgt
    24541 tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc gaagcaatgg
    24601 acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact agaaatctac
    24661 ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg cattcggata
    24721 atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct tccaaccctt
    24781 atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt gttttcctag
    24841 aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct tacgaagttc
    24901 ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc acatacggag
    24961 caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat atatctgttg
    25021 aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt caatccgtgg
    25081 aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc gttgccgcgc
    25141 aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca atcggacgag
    25201 tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg ttgccaaatg
    25261 caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag aagggacgaa
    25321 acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc gcagaaggtg
    25381 aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat acattactag
    25441 aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga acggttaaaa
    25501 atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta gaaatcgatg
    25561 caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc caaactccag
    25621 ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca agcgaaggag
    25681 tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa atcgtcaaat
    25741 ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa gttcaaaatg
    25801 acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac gtgattgttc
    25861 cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct cctggacaca
    25921 atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat
    25981 caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg gctatgccat
    26041 ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact gcaactggaa
    26101 aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg
    26161 caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct cctgcagcag
    26221 aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac ttggaagaac
    26281 gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac agtaagcata
    26341 cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg gataatcgga
    26401 aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg ccatatgtcg
    26461 tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct atcgacgatg
    26521 caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact gatacggatc
    26581 acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt gctattgcag
    26641 acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca gaagatatgc
    26701 gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg tttacaccag
    26761 tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa caaccaatcg
    26821 taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc
    26881 accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa cgtcttttag
    26941 ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg agattttgct
    27001 gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc tgccatccgc
    27061 gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg cggtgtttgt
    27121 ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca ttatcaagag
    27181 tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa
    27241 acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt tggtttccta
    27301 ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca
    27361 ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc aatcatcgca
    27421 acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt gttagattct
    27481 actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg tggcggtgtg
    27541 attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac gattcttgaa
    27601 ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt agaagatgat
    27661 ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac
    27721 ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt tactgctgat
    27781 tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt agaacaagcg
    27841 ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg tacgaacgta
    27901 cctaacatct atgccatcgg tgatatcgtt ccaggggcgg cattagcaca caaagccagt
    27961 tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt agattataaa
    28021 gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat gacgatcaaa
    28081 gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc agggaacggt
    28141 cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac gatcgaagat
    28201 aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta
    28261 gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat tcatcctcat
    28321 ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt accaatccat
    28381 atttgatttg aataatacag gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta
    28441 gaattatcaa taatattatt atgtaaactt aaaaatatga caacttcttg gtttattacc
    28501 acttcctatg ctacactgaa gaggaatatg aaataggaga gttgaagatg caagaatatc
    28561 agtatccact tgatttagat tggacaacag acgaaatggt cattgtaatg aagatgtggg
    28621 aagatctgga acgtgcgaat gaaaaaggga taaacaatga agcttttttg aagacttatc
    28681 agcagtttaa aactgttgtc aaatccattg gtgaggaaaa acgattggga cgggaatttg
    28741 aaaaagcttc cggttattct ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca
    28801 aaactttgag aatgaacggg tgagataaat ggaacagatg attgatcata tcaagtcctg
    28861 gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa
    28921 atcaaatcga acggatctag taacaaatgt cgatcgaaac atccagaatt ttctaatcga
    28981 aaaaatccat tcctttgatc cagaggcgaa aatattggca gaagaagaag ggtgtaatca
    29041 attatcggat ctcgatggaa gagtctttat catcgatcct attgatggca ccttgaattt
    29101 tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt tatgaatccg gcgttggaaa
    29161 gcttggtttc atttatgacg tgatgagaaa tgaacttttt tggggtggaa aaggaatagg
    29221 cgtgtataaa aatgatgagc cattgcctag accagcaaat aaatccttat cggaaggctt
    29281 acttggtgtg aatagttatc ttttcggtca taataaatat ggaatccgag aaattgcgga
    29341 aagcagcatg gggacacgta tgtacggatg cgctggtctt gaactagtgg caatcttgaa
    29401 aggaaaccac attgcttata tttcaaacct tagcccttgg gattatgcag cgggaaatgt
    29461 cttattggaa gagtttggta tggtaacaag cggtcttttt ggtactcctt tgacatttga
    29521 aaaaagggaa tactatttag cagggacttc ttgtgcatat gcagaaatcg cagacctttt
    29581 gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa
    29641 agattaattt tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa aagtctgcta
    29701 taattagcag tcgagtaaaa tttgccctgt cataaagaac caataaaaaa ggagttagtt
    29761 acattgaatt atagaaacga tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga
    29821 aaaacaacat tagttgatga actattaaaa caatcagata cattagacgc acatacgcaa
    29881 ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta
    29941 gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa acatcatgga tactccagga
    30001 cacgcggact tcggtggcga agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg
    30061 gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa aaaagcactg
    30121 gaacaacata tcacaccaat cgttgtcgta aacaagattg acaaaccttc tgctcgtcct
    30181 gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat taggagcaga tgatgatcag
    30241 ttagactttc ctgtgattta tgcatctgca ctaaatggta cgtcaagctt gtcagatgat
    30301 ccagcagatc aagaaccaac catggcacct atttttgata caattattga aaaaatccct
    30361 gctcctgtcg ataacagcga cgaaccatta caattccaag tttcattact tgactacaac
    30421 gattatgtag gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa agtaggagac
    30481 caagtagcat taattaaatt agacggaaca gttaagaaat tccgtgtaac gaaattattt
    30541 ggtttctttg gactaaaacg tctagagatt caagaagcaa aagctggaga cttgattgct
    30601 gtctcaggta tggaagatat ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat
    30661 gcgttaccta tccttcacat tgacgaacca acattgcaaa tgactttctt agttaataat
    30721 tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc gtaagattga agaacgtttg
    30781 atggcagaac ttcaaacaga tgtatcctta cgagttgaac caacaaactc tccagatgca
    30841 tggacagttt ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa catgcgccgt
    30901 gagggatacg aattgcaagt atctcgtcca gaagttattg aaaaagaaat cgacggtgta
    30961 aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag aagaatatat gggtagtgtt
    31021 atcgagtcct tgagcttacg taaaggtgaa atgcaagata tggtccatac aggaaacgga
    31081 caaatccgtt tgacattctt aacacctgcc cgcggtttga ttggttattc aactgaattc
    31141 ctttcaatga cacgtggtta cggaatcatg aaccatactt ttgaccaata tctgccaatg
    31201 cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa
    31261 gcaacgactt attctatcat gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt
    31321 acagaagtat atgaaggaat gattatcggg gaaaacagcc gtgataatga tttgactgtc
    31381 aacatcacga aagcaaaaca aatgacaaac gtacggtctg ctactaaaga ccaaacatct
    31441 gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt tggaattctt gaatgatgat
    31501 gaatattgcg aagtaacacc agaaagcatt cgcttgagaa aacaaatttt agagaaaaat
    31561 gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag agagaaacca
    31621 gcttgttata aaaaatagct ggtttctttt tttgtccaac tcttttctgt ggagaagtca
    31681 tctttgtcaa tggacataaa ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa
    31741 aaacttattt ctagagagtt tttgtttcat aataagataa tgtttcacga aaggagatca
    31801 acttgtcctt cgaagaaaat cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg
    31861 acttattaaa acaatacttt ggctatgatg agtttagacc tggtcaaaaa gaaattatcc
    31921 agaaagtcat tgatcaggaa aatgtattag gaatcatgcc taccggaagt gggaaatcaa
    31981 tctgttacca gcttccagct ttactattag atgggcttac tgtggttgtg tcccctttaa
    32041 tctctttgat gaaggatcag gtggacgcgg cgaatcaatt aggtataccc gccactttta
    32101 tcaatagctc gttagatggt tacgaaacag ctagacgctt ccaagaaatc gatagacagc
    32161 agtaccgttt actttatatc gctcctgaac gttttatcat gccggatttt atccaagcaa
    32221 tgaagcgatg gaacgtccgt atgatcgcta ttgacgaggc acattgtatc tctcagtggg
    32281 ggcatgattt ccgcccaagt tatttacaga tggcgaacca gctagatcaa ctgccaaatc
    32341 gtcctgtaat tgttgctttg acagctacag cgactgtgca agtagctgct gacataaaaa
    32401 gacttttaaa aattccagaa aacaaccata ttcagactgg ttttgaacga gaaaatctac
    32461 gatttcaagt aattaaagat caaaaaaaag aacaatatct aatcgaatat ttaaagatca
    32521 acaaaaatca atctggtatc atttatgcag caacgagaaa agaagtagac cgtctctatc
    32581 atttgcttaa aaaattcgat ttttccgtgg gtagatatca tggtggtcta aatgagaatg
    32641 agcgaacaga aatgcaagaa gcctttttat acgatcgctt gcagctgatt gtagcgacaa
    32701 atgcatttgg tatgggtata aataaaagca atgtccgttt tgttatccat tatcaaattc
    32761 ctggctctct agaagcatac tatcaggaag ctggtcgggc gggaagagat ggtctttcca
    32821 gtgaagccat cctgcttttc tctcctcaag atatccaagt ccaaaagttt tttatccagc
    32881 agtcccaaag agaagaaggg caaaagcaaa aagaatatga aaaattaaaa gcgatgacag
    32941 aatacgtgta tattgagtcc tgtcttcagc aatacatcct aaactatttt ggcgagacaa
    33001 gcagtccgtg caaccgttgt ggtaactgtt tagatgacag agaaatagta gaagtaacga
    33061 cagaagcaca gatggttctt tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa
    33121 tgctgatgaa agtcttagca ggatcgaaag aacaaaagct gcaagcttta ggttttggac
    33181 atttatctac ctatggactg atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg
    33241 aatatttgat atcgaatggc tatcttctga ccatcaatgg tgagtatcct gttttgaaag
    33301 taacagaaag agggatacaa gtattgaaag gacaagaatc tgtttatcga aaggaaccca
    33361 agaaagtcca gcagctatcg gatgaagaaa ccgatacact atttgaagtg ctgcgggaac
    33421 tgcgaacgga tctagcatca gaagcaggcg ttcccccgta tgtcgtattt tcagattcca
    33481 cattgaaaga aatgagccga atacgtccat caagcagatt ggagatgctt cagataaaag
    33541 gagttggcca aagcaagctt gataaatatg gcgaagcctt tttatctcgg ataaaaaatg
    33601 cggactctaa cgttctggat aagtagaaag aacgaatacg taaaaatgaa taaaaacaac
    33661 tggttaaatc tgtttttttt gctccttatg aaaatcaaat atgttataat taaggaatta
    33721 gaatgggagg taagcacaga tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa
    33781 tgatgaagca gaacggatca aacgattaat ccgaaatcaa aacaacagtc tctgtatctc
    33841 acaatgcaaa gccttcgaag aagtggtaga tacacagatg tacggctttt cccggcaagt
    33901 cggttttgct gtccgtatag gagtaatcgg aaagaacgag ggccagctga tgttaagtga
    33961 gctagaacga gaattgaatc agttatatag tgaggtttac caagaaattt atgataaaaa
    34021 agaagttggc aaggaggaat agacttgcct aagaaagtga aaaaaaggca tctattagac
    34081 tatagcatct tgattcccta tttaatttta tgtgtattgg gattgatcat ggtatatagt
    34141 tctacgtcgt atctattatt agaaaacgga caaaatccgt ccgcatcagt cattaaccag
    34201 agtatttttt gggtgcttag cttgattgct atcgcactac tttataaaat gaaaacagat
    34261 gttttgaaaa accagcgttt gatcatggca gccatcgcag ttttgacgat tttgctgctg
    34321 attgttgtgt tttttggtaa ggagatcaat ggggcgaaag gttggctgca aattgcagga
    34381 ttctcgatcc aacctgccga atacctaaaa atcatttcta tctggtacct gtctctgact
    34441 ttatctaaaa gacaaaacag tgtacaaaaa gattttcttg ggactgtgaa acgtccactt
    34501 gcaatggtta tcggacttac tgcattagtc gctattcttc ccgattttgg taatgcggca
    34561 gttatctttt tgattatttt ggttctgctt ttagccagcg gagtcaatta tgtgtatact
    34621 ttgattgtag gagtaggagg cttttgtcta agtactttta cgatctggct gatcaatatc
    34681 acaaacggaa agatcttacc cggacgttta cagtatatct ataatcgatt tgctatttac
    34741 caaaatcctt tctcagacga attaaataaa gggcatcaac tagtcaacgg ctactatgcc
    34801 atgttcaacg gaggactatt tggcagggga ttaggtaaca gtatccagaa aaaaggattt
    34861 ttacaagaag cacaaaccga ttttatttat gcaatcgtag tggaagaact gggagtgatt
    34921 atggggattt tgatattagc gttgctattc tttatgattg tgcgcattgt ccttgtaggt
    34981 atccgttcaa aagatccgtt caattcgctt ttgtgcattg ggatcggcgc aatgtttttg
    35041 atccaagtat ttgtcaatct tggaggaatt actggcgtta ttcctttgac agggatcacg
    35101 ttcccatttt taagtcaagg aggctccagt ttgctgatgc tttcgatttg tgtcggcttt
    35161 gtcctcaaca tcagtgcgga tgaaaaacgc aagagcttag gtttataata aaaaaagatg
    35221 taggagtggt tcgatgaaaa aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt
    35281 atttcgtgct tgtacagaat taggaattaa aactgttgga atctatgcag aagaagatga
    35341 atattccgta caccgtttca aagcggatga agcatattta gtaggacaag gaaaaaaacc
    35401 gattgatgcg tatctagata ttgaagggat catttctatc gcgaaagaat gcggagctga
    35461 cgctattcat ccgggttacg gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga
    35521 agaagaaggc atcatttttg ttggacccaa actgcatcat ttagatattt ttggagataa
    35581 aatcaaagca aaagctgcag ctatcgaagc ggggattgct tcgattccag gcacagatgg
    35641 accaatcgct tctatagacg atgccttgga gtttgcgaaa caatacggtt atccgattat
    35701 gatcaaggca gctctaggcg gcggcggacg cggcatgcgt gtagctcacg atgaaaagag
    35761 tgcaagagag ggctacgaac gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga
    35821 agtgtatgtt gaaaaataca tcgctaatcc taagcatatc gaagtacaga ttctcgggga
    35881 tacgcatgga aacgtcatcc atctttttga aagagattgt tctgttcagc gccgtcacca
    35941 aaaagtagta gaagtagcgc cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg
    36001 ccaggcagct gtccaattga tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt
    36061 tcttgttgaa ggagatgatt tttactttat cgaagtcaac cctcgcgtcc aagtggaaca
    36121 tacgatcaca gaaatgatta cagatattga tatcgtaaca acgcaactgt tgattgctca
    36181 aggtttggat ctgcataaag aaatcggatt gcctcagcaa gaaggaatta aattaaatgg
    36241 ttcagccatt caatgccgaa tcactacaga agacccgttg aataactttt taccagacac
    36301 agggaaaatc gatacgtatc gttcacctgg tggttttggc gtacgtctgg atgtcggaaa
    36361 tgcttacgcg ggctatgtag tgacaccgta ttttgattca ttgctagtaa aagtatgtac
    36421 gcatggtgct acctttgaaa cagccattca gaaaatggaa cgttgcttga aagaatttag
    36481 aatccgtgga gtgaaaacaa acattccatt tatgctcaat gtcatcacac atcctgaatt
    36541 ccaatcagga aatgcaaaaa caaccttcat tgatagtaca gcaacattat ttgaatttcc
    36601 tcgactacga gaccgcggaa acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg
    36661 ttttccaggt atcgaaagtg gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga
    36721 tctaatcaca agaactgatt acgtaacggc taagaatgta ttagatgcga agggtgccga
    36781 tgctttggtc gaatggatca aaggacagga aaatttgttg ttgacggata cgacatttag
    36841 agacgcccat caaagtctgc tagcaactcg tgttcggaca aaggatttca agcagattgc
    36901 acgtttgact ggagaaggtc ttcctgaact cttttcaagt gagatgtggg ggggagcaac
    36961 atttgatgtg gcttaccgct ttttgaatga agatccttgg caacggttga gaaaaattcg
    37021 ttctttaatg cctaatacgt tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta
    37081 ttctaattat cctgacaatg tattggtgga atttgtcaaa gaagcagcag cgcaaggaat
    37141 cgatgtattt cgaatctttg atagtttgaa ctggactcca caaatggaaa aaagtatcca
    37201 agctgtacgg gatactggta aaatcgcaga ggcagctatt tgctatactg gcgatatcaa
    37261 cgatccaagt cgggcaaaat acaatgttca gtattataaa gacatggcaa aggaactaga
    37321 acaactgggt gcgcatatca ttgcaatcaa agatatggct gggctgctta aaccgcaggc
    37381 agcttatcgt ttgattagtg aattgaaaga aacgacagat ctgccaatcc atctgcatac
    37441 tcatgatacc agcgggaatg gaatcatcac ttattctgct gcttcaaaag caggagtaga
    37501 catcgttgac gtggcaatga gtgcaatgag tgggaatact agtcagccta gcatgagcag
    37561 cttgtattat gcactggtca atggtccacg gctgccagaa atcacgattg aaaatgcaca
    37621 gaaattaaac cattattggg aagatgtgcg catgtattac aaaccgtttg aaaacggctt
    37681 gaatgctcct gaaacagaag tatatatgca tgaaatgcca ggaggacagt attcgaatct
    37741 acagcaacag gcaaaagctg tcggattagg tcatcgctgg gatgaaatca aacaaatgta
    37801 tcatactgtc aacttgatgt ttggcgatat cgttaaagta actccatcat ctaaagtagt
    37861 tggagatatg gcactattta tggtccaaaa tgatcttaca gaagaagata tttatgaaaa
    37921 aggcgaaaca ctaagtttcc ctgaatctgt tgtgactttc ttccaaggag aactagggca
    37981 acccgttggc ggatttccta aaaaattgca aaagattatt cttaaaggca gacctgcatt
    38041 gaatgaacgt ccaggtcttt tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt
    38101 ggcagaaaaa attggctatg aaccaaaaca agacgaagta ttaagctatt tgatgtatcc
    38161 gcaagtcttt ttggactatc aaaaagcata taatcaattt ggcgatgtta cattactgga
    38221 cacaccaaca tttttccaag gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg
    38281 cggaaaaacg cttatcattc gtttagatga aatcggagaa cccgatatcg aagggaatcg
    38341 cgtactgttc ttcaatttga atggacaaag acgtgaaatc actatcaaag ataattcgat
    38401 catcagtacg gtccaaaccc gattgaaagc tgaaccgaca aaccgagaac aaattggagc
    38461 aacgatgtct ggttctgttt tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg
    38521 tgatacgttg atgattactg aagcgatgaa aatggaaacg gccatcgaag cacgttttga
    38581 cggagaagtc gcacatgtat atgtgtcttc aggagacact atttcgtctg gtgacctttt
    38641 gattgaagtg actgagaaat aataagcgaa aggtggggaa acatgaaaag actgggtttg
    38701 ttttggctga ttctgctatt agttattgtg gcattctaca tggaaccagt cttgtttccg
    38761 ccaaaacttg aagtgaagga aaactctgat caacaaatat caaaccaaat gacaacgtgg
    38821 aaataccagg agcttaaagc aactggtttt tctacttata tcggaaaacc gatcgatcag
    38881 ctggaaaagg attttggcca agcctatgat cgattaacta gcggatttgg ttttgaaaca
    38941 agatattatg aaaataaagc tagtcatctc acatttgaag cgaacatcca agacagcaga
    39001 gtcacaactg ttaaagtact gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg
    39061 atgacgatgc aagatcttac gaaaatcact actatctata ctaatttcac ttttgagtac
    39121 catgatacag aagttggaat cgaactaatg gaagaggata tgaattatcg tccgctaatt
    39181 gcttttgata acgacacttt tgctatttta ttttttgatc aaacaacaaa cggcctattt
    39241 tcagttgtgt atttagataa agatagtctt ttgaaactat tgccatatca agtgtttggt
    39301 gaaggccttc cacattatca agcagataaa tcggctgatt gggaatcaat caatcgcgtt
    39361 aaagaacgaa aaagtacgac tttattaaat atgcttagaa gagaagacga acttcccata
    39421 tacaatcaaa cacttgcctt tcaaaaccac agtcagctgc tactacatga ttttttgaca
    39481 aggcctgagg aaatattgtc agaggaacgt atggctgatt ggcaaaaatc gctaggcagt
    39541 gctcaaacga cagtgaaatt ctcactgacg aatgatgaat tggagaagtt aatcagtaaa
    39601 caaaaattag aaaatactag tgggctattt attcatccgg cgattgacgc tactttttcg
    39661 tttttatatt ggtacagcga tccttacaac catgaccgtt tcatggaccc gggaacggat
    39721 tctgtaggca ttgctttttc taaagaaaat atgttagtct tattgcaaga agaaaacgga
    39781 gaaagtagtg atagcagtga tcgttaacga agagctgttt caactggagg atcaatgcct
    39841 ccaagtcgct gaaatgataa aaaagagtaa aacgatggct tcttatcttg aacataaaca
    39901 gtcaatggaa gaatctgcaa aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga
    39961 agcatttgaa aatgtagcgg catatggtga gtatgcacct gattatcgtg aaaaaaaacg
    40021 agctgttcga aaagcaaaaa gacagcttga tatggatgag catgttgcag ctttccgata
    40081 tagtgaaaca cagctgcaaa gtctgttgga tcaaattgtc caagtacttg ccgaagaagt
    40141 ttctaatgaa atcaaagtag atgcaggtag cccgtttttt gaaacgaaaa aacactttgg
    40201 atgcggaggt aattgtcatg caattagaca atagtgaatt tgaaataaca aaacgacgcg
    40261 ggctgatcgt atgggtctac agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt
    40321 tgcattatgt ctcaagaaga atgaaatatg tcgtgcttta cttaaatgaa gagacattgg
    40381 aagaaacaga agaacgaatc cgtaaattac attttgttcg aaacgtagaa cgttcttacc
    40441 gaccggatat cgaaatgaat tttgccgaaa aaataggaaa caaaacagta ttaaaagacg
    40501 aaggtttcga aattgaagag ctaaatacag aaatccgatt agctgaacat ctttaaaaat
    40561 ctcttatgag cagtttttaa ataaaaaata acttggataa cgatcgattt ttgatcattg
    40621 tccaagtatt tttttggtat actagtacca gtaccatttc taaggagtga gtagatgcgg
    40681 ataatatcag gaaattatgg cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga
    40741 ccaactacag ataaagtaaa agaatccata ttcaatatga ttggaccata ttttgatgga
    40801 gagacagtat tggatctgtt tgcaggaagc ggtggtttag ctatcgaagc agtatctaga
    40861 ggctgcagtc atgcggtatg cgtagacaaa aattaccaag cgctaaaaat catcaaagaa
    40921 aacatcgaaa ttacgaaaga accagagaaa tttaccacaa taaaacttga tgcggacaaa
    40981 gcaatcgaac ggctagcagc ggagaaacaa caattcgact acttgtttct tgatccgcca
    41041 tatgcaaaac aaaaaataat cgatcaaatc cataaaatgg aagaactggg acttttttct
    41101 aaaaccgcag ttatcgtatg cgaaacggat aaaacagtcg aattgccaga aactgtggct
    41161 ggattccatc aaataagaaa acagatttat ggcataacgg ccgtaactat ctatagaaaa
    41221 gagggaaatc aatgaaacgt gcattattcc caggaagttt tgatcctttt acaaaaggtc
    41281 acttagatac ggtggaacgt gcggctaaat tattcgatga ggtagtaatt ggtgttttta
    41341 tcaatacttc aaagaaaagt ttgtttcccc cagaagaaag aatgacattg atcacaaaag
    41401 ctgtttctca tcttcctaat gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa
    41461 cagcaaaaga gattggtgca gatgcgttga tcagaggcat acgaagtatc aaagactttg
    41521 aatatgaacg agagattgct cagatgaatc atcatctata tgatgaattg gaaactgtct
    41581 tcttattagc aaaaccagaa tatagtcacg tgagttccag tattttaaaa gaagtactgc
    41641 actttggcgg agatgtgagc agctacttac cgccagtgat caacgaagct ttagctggaa
    41701 agcgtgaatc aaatgaaagc taaacaatgg ttgaaaacag tactgattat catatttgga
    41761 atgttgatca ttggatttgt agtagttccc ttgccgtatt atgtagaatc accgggagcc
    41821 acgatcgatc tgaaagaact aattacggtc aatgataagg aagataaagg ttctgggtct
    41881 ttctctctta cctctgttgg tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa
    41941 atgacccctt ttgaagaatt gatcagtgaa gaagaattga ctggtggggc gacaaatgaa
    42001 gaatataatc aaatgcaaca gtattatatg gattcttccc aaaatgcagc aatcgaacaa
    42061 gcgttaaaac ttgcagacaa gccttataaa atggaattca aaggcgtata tgtcatgagt
    42121 gtagagaaaa catcgaattt ctttggcaag ctttcagttg gagatacagt aacaaaagta
    42181 gacgggaaaa gctttcaaag tacagaagca tttatggatt atgtaaaaag ccaaaaagtt
    42241 ggacaaacaa tcgaaatcag ttatttgcat aatggtgaag aaaaaacagc ttctggtgat
    42301 ctgatcgagt taccaacaga taaaaaggca ggaataggta tcacgcttac tgaccatacc
    42361 gagatcgaat cagatacaga tgttcgtatt aattctggct ctatcggtgg gccatcagcg
    42421 ggattgatgt tcactttaga aatctatgaa caagtcactg gtaaaaactt gcgacatggt
    42481 aagcaaattg caggaactgg aacgatcaac agtgaaggag aagttggccg aatcggagga
    42541 atcgataaaa aagtggcaag tgcggataaa gctggcgtag agattttctt tgcaccagat
    42601 gatacgattt cagacgatgt taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa
    42661 gcaaaagctg ccgctaaaaa attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta
    42721 caagaagcgc ttgactatct agagaaaatg aaatgaaact aaataattaa agaggggttg
    42781 tgacacgagt tctgtcacaa cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa
    42841 acaaaagaaa aaattgacgt aattagatgt tgaagggacg attttgccag agctcactgt
    42901 ttcttcaatt ttaaatgaaa ggagtaatga atgattttta ctggtcattc tttgaaaaaa
    42961 gaacaatgat agaaaaaatc agcaagaaaa tactagcttc acccaaattg atcatttgga
    43021 tgtctatgat agttcttatc ttttgtatcg caagtggttt tttattatca aatttgctat
    43081 tttatagaga aacagaagag actgctgcaa aagaaatact ttcttcgttt agttccagtg
    43141 gagaagatcc atcagctgaa ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt
    43201 acaccgatat caaaggaagt gtgaaggagc ctggtatcta ttccttttct tcagaagaac
    43261 gggtctatga tgtactaaag cgtgcaggtg gattattaga agaagcggat agcgatcgaa
    43321 tcaacttttc tgcgaagata gaagatcagc aagtcctata tatccctgct gtaggtgaag
    43381 agctgccaga acatcttgat cagttggcat cctcggaagg aaaacagcct acagcagatt
    43441 ccgagcctcc caaaataaat atcaatacag ccactccatc agaacttcag cagattcctg
    43501 ggatcggatc agtcaaagcg caagaaatca ttcgatttag agaagaaaac ggatccttcc
    43561 agaaagtgga agacctgcag gagatttctg gtatcggtga aaagacagtt gaaaaactaa
    43621 aaaacttcgt tacaattaaa tagaatgatt caaaatcgac tggcacgagc aataaatgga
    43681 cgctaatgga tactaagaat aatccaattc taagagcttg gcaggacgag gaggtagtat
    43741 ttttggatag gaaatagctt gattgttcgc aaagaaacat taaaaaaacc aatgctgaat
    43801 gtctatctcc aaaataaaat atctgggatt catattatga atacggctgt cagcggcaat
    43861 aacagtcaag ctctacgaga acgctttgcc aaagatgtac ttagctatac agcagataaa
    43921 gtatttattc tcattgggac gaatgatcta gcagagcata aacaactttc taaagaaacg
    43981 taccaaaaaa tctgcagtgg atgatagttc agctgaaaaa agactatagt tctgatcatg
    44041 ctttctttat cacgccacca gcagtcgatg agacaaaaca aaaatatcgg aataacaagt
    44101 tgatcaaaga atatatctct gttctcacag aagtagcaaa acgccatgat tgtgaagtat
    44161 cgatttttat acggaatttc ttgaacaaat ggattcgaag aatttttata atgggctaat
    44221 ggatgacgga cttcatttcg gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg
    44281 ttatatttaa agaatatttt gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg
    44341 gaggaatata aaacatgtta gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag
    44401 ttactgataa gtcacataat gaacgcattc cttgggacca gtactttatg gctcaggcag
    44461 tactgttatc tttacgtagt acgtgtacac gtttagaagt aggagcgaca ctcgttcgag
    44521 aaaaaaggat cattgctggc ggctataatg gagcagtcag tggagatgtc cactgcatag
    44581 atgaagggtg ttacgtcgtg gatggacact gcttgcgaac gatccatgca gagatgaatg
    44641 cactcctaca gtgtgcaaaa cttggtatcc cgacggaagg ttcagaaatc tatgtcacac
    44701 attttccatg tttggcctgt acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt
    44761 atctccatga ctatcgaaat gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat
    44821 ctgtacacca agttgcttta gactcacagc atttttcaaa acttcaaaat gaattaacag
    44881 gaaaacatgt ctagcgtttt agaaaggcat gttcgaaatc ggttgatttt tccggcaatt
    44941 acgtcgatga ttatctgttt gttgtattac actgacaatt ggggttatcg tttgcttctt
    45001 gccggaattt ttttatggtt ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt
    45061 ttgtgtagca taacggtatc catttcttat aggctacaag agtattcatt tttagaggaa
    45121 aaaacaacat ctgcgcaaat tactgtattt acagatacga ttcgtgcaaa tggtaatttt
    45181 ttgacaatgg aaggaaggtt atcagagaat aaacaaaagc tccagctttc ttatcaagta
    45241 aacacagaag cagaattgaa aacatggctt aacttccgag gaaagggaat agaattgact
    45301 gctgaaggcg agttcatctc tccagcaaaa aagcggaatc tttataattt tgaccaagaa
    45361 gcttacttca ggaaccatag aatttctgga cggttccaaa ttcaaaagat tgaacagatc
    45421 accatgcaaa atcactggcg aaactggctt cagagaaaaa gaggatcttt tatctccttt
    45481 gtccaatctg aatttccgca aaaaacaagt gtatatatta attctttatt atggggctat
    45541 aaagatgcag gattcagaga accagaagaa atttttaggg agagtggttt gctgcatctg
    45601 ttcagtctat cgggtctaca catccaattt tatttagggt ggctgtatta tttgtttcga
    45661 cgtatcggat ggccactaca cgtcagtatt attccgttga gtctactaac tatctgttat
    45721 gtttctattg caggaagttc tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga
    45781 ttgctagtca aattgctgaa taagcattat tccaccatgg acttatttgc agttgtcttt
    45841 tggcttcttt tactatttga tcctcgattt ctttttcatg cgggtggaca gcttagtttt
    45901 ttcatgagtt ttttgtttat cctgatttct tttgatggag attggttgaa aaaaacggcc
    45961 tctcacgtcc tgtttactat gatcacgatg ccgttgattg tatggcattt ttatgagtgg
    46021 cctattttag gtagtctttt tacattactg attgcaccgg tattcaagtt tctttttctt
    46081 cctggtgtgt tatttctcac actcttcaat cgctggctgc cggaaacttt acttttgctg
    46141 atagaagagg gattaatctt atttgagcga ataatcgatt tttcttcagg gtcgtctctc
    46201 attataggac gtttgcccct tgtgctatct gggatgtata ttttcggatt actgatttgt
    46261 atggataggt tgaaagaaca acctgtgaaa cacttgtttt atgttggtct tttttctttt
    46321 atttttttga ctttgccatt tttacgcttt tcttcaacga tcgcttttat cgatgtcggt
    46381 caaggagaca gtatcttttt gcaaagtcct tttcgaaaag aaacgatttt gatcgatact
    46441 ggaggtcagt tacactttga aagagaaaag tgggcgcaag gtatgatacg tcctcctgct
    46501 gaaagaaata tcgttccata tctaaaaggt caggggataa gtgtaatcga taaactcatc
    46561 ttgacacacg acgatacgga tcatgtggga gaattaccca cgttatctca gcattttact
    46621 atcaaaaaaa tttatatcgg ttggggcgct ggacgaaatg agccattaca aaggcattta
    46681 tctgaagctc aaaaaaatgg tactgagatc attgaaatca aacatgggga caggatcagc
    46741 ggttattatg atctttatgt attgactcct tttgaaaagg gagagggaag aaatgaagat
    46801 tcgattggtc tatggatgga atataacaat aggcgctttt tatttttagg agatttgaat
    46861 caagcaatgg aaaaacaatt gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa
    46921 ttaggtcatc acggtagccg tacgtcaagt aacacagatt ttctttccca tattgaagca
    46981 aaacatggga ttatatcctg cggcgtaaat aatcgttatg gtcacccgca cagcgaagta
    47041 ttggagacac tagaagaaaa tcagataaaa acatggcgaa cggatcaaca aggaatgatc
    47101 acctatcgct ggcatccagt ttttcatcct catgggctag tggaaacaat gatagactag
    47161 taattctcat ctgctcatgg taggataatt tttgaaagtg gtgagtaatt tgagtataca
    47221 agcagagtta caaaaaatac gtaaagaaaa acttgcatct tgttacttga ttttaggaac
    47281 agaaaagttt ctccaagatc aagttcggac agaaattgtc aaaaaaatcc aattagaggg
    47341 cgaagatgac ttaaattttt taagttttga catggaaaat gcaagtttag atgaggtagt
    47401 agctgaagca gagacgttgc cgtttttcgg agatcaacgg ttagtattcg tagagaatcc
    47461 ttatttttta acaggtgaaa aagtaaacaa tggaatcgaa caaaatactg atttattgac
    47521 ggcttatttg aaagaaccat tagaatcgac tgtattagta ttctttgcgc catatgaaaa
    47581 gttggatgaa cggaaaaaag taacgaaaca attgaaaaaa acagcaatta tcatcaatgt
    47641 ccaacagttg aatgaaaaag aagtacgtca atatctgatg aataccttag agaattcctc
    47701 aatcaaaatg gaacgaagtg caattgattt attcttgagg ttgaccgact tagatttatc
    47761 caaaatgatg agagagttac agaaattggt cctttatgga caaaatcaac agcaaatcac
    47821 ggtaagagaa gtagaagaat tagttcctaa aacgttagaa cataatattt ttgacatgac
    47881 ccaatatatt ttaaaaggga agacggaaca agctttgcgt ttatacgaag atttagttat
    47941 gcaaggggaa gaaacaatca aaattaatgc gattttgctt tctcaattac gtttattttt
    48001 acagacaaaa tttcttgtaa agattggtta tcagcaggca aatattgctg aaacattgaa
    48061 aatccatcca tatcgtgtaa aacttgccat gcaggaagta aaaaaattcg atgaacattt
    48121 gctggtacag ttgtttgatc aattagttga gatggattat caaatcaaaa cgggacaaat
    48181 cgaaaaagaa ttaagttttc aactattcgt tttgcgcaca gggcagttgt taagaaatcg
    48241 ataagaagaa agacgagggc aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg
    48301 acaaataaaa aaatcggccg aagccgattc ttttatttag cgattttttt gcttaaacga
    48361 gatttgtcgc ggttagcttt gtttttgtgg attagacctt ttgattcagc catatcaata
    48421 gctttaacag cttctttata taacgcatct acgttgtcag cacctgaagc tacagcatct
    48481 tcaaattttt tgattgcagt acgcatagcg cttgtttgag atgaattttt cacattagcg
    48541 ttttcgcttg tacgaacacg tttgattgca gattcaatgt ttggcattag tttcacctcc
    48601 gataacttaa gttcttacgc gtgttaccgc ataattcaac attcattatt atacctaaag
    48661 gcagcgtgct ttgcaatatg aacatgacaa gtttttttcc ttaacagtct tttattagga
    48721 ttttttttca gtaaaaacgg atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg
    48781 gaggcaatag cgtcaaacca aactagattt ttatttatct atttaaacaa agtttgaatg
    48841 ggaatgtttg gtactaagtt attatataac aaagagaacc gtttgcaaag ttttttttga
    48901 aaagggatac gattccctgt tgttctttgt ttttctatta agaaactgca gttattttat
    48961 aaccagtatc ctttgatcag taaaggctat tctggtgtac cgattatttt aatgtattca
    49021 tcgatatttt ttatcaacat tttttttatg accaactggt tccgacggtt ccatttgcca
    49081 cttacttcca gcacatattt atttaggggc aaacgtaaga tatattttgc caaatagcga
    49141 tctttaagca ggccattgat cacacggtcc tcaagctgca agttgatccc agtcagcatc
    49201 attggctgtt cttgtaaaac gtacgtatca attaaaagac cttcaaaagt ttccacaagc
    49261 gtcttccttt cttttcaaag taacctgaaa acaggcacta attcttccat tttgatattc
    49321 gttgaagtgg atgcgatcat tctttctttt gtccactgta atctagtata acaaaaaaga
    49381 gcagaagagc caaaaagaaa aaagagcatc gaattctata ggaagtctca ttggcacggc
    49441 ctaaattatt acaaaacatt gattaaaaaa agcccctgca tcgcaggagc atcccctata
    49501 tcgttttaat aggtcaatca aaaagcagta tcaaacacat tttgtatcaa atcaggaaat
    49561 aatagataag ctaagcccag aatcaaaagt accgctagaa gaaaccagcc gattgttcct
    49621 atgaaatact ttaatttttc gaacattgta tcacctcgtt gtcagtatat cacggatcag
    49681 taaattgggg gtgttaagaa tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc
    49741 aaatacttgt aagatacagt aagtgatacc agaagcaatg accggtccgg cggcgattcc
    49801 tcttaagaag acaacaccta aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc
    49861 agaagcaatc aatcctacac ccttggaaga taagacagcc actagcccac cgcaaaaaac
    49921 agctacccag ccaatagggg atttaaaggc ttctaataga tcacgtagac cgatttctcc
    49981 tgttgcgata gggattagaa tagccgctgt gatgatcgtc accccaatat tgattccttt
    50041 gccttgaagc caagggaaaa aggaacggga ggctggtatg agcttcataa tcaaaacaaa
    50101 agctgccgct atcatcagac tttgattttt agctaaaatt gctacaagta aaataattcc
    50161 taaaaaaagc caactttcca tgaaatatat tcctcacttt cttcgaaaca atcataacat
    50221 gtttttagaa agttacaaaa gtaggaattc atccttcaag acctattcat aagctgtttt
    50281 tttcataaaa taaacaaaat cataaaaaaa cactttgctt atttgacaga agacagccgc
    50341 gctttgtata gtggacaaga cttacatttt tttgaagaat cagaaagatc aggtaaaggt
    50401 cttagtaaac gcaagccatt cagaataacc aatattgtgc ttccttcgtg tcctacaact
    50461 ccaagaggta gatttaaaaa ttgcaggaag ttagaagcaa tcaacaagag aataacaaga
    50521 atcgaaaaaa taatattttg tttgatgatc cgccgcattt ttaaagatag ccgatgactg
    50581 tagacaaatt tatttaagtc gcttttcatt aagacgatgt ctgcgatatc catagcggca
    50641 tctgtccctt gccccatagc gacacctaaa gaagcggctg ctaaagcagg cgcatcattg
    50701 actccatcac caaccatcgc atttacttgg taaagttcat tctctttttt gactagttgt
    50761 gttttttctt ccggagtaca gcttgcatag taatgttcaa tccctacttg ttcagcaatg
    50821 gcctttgctg tgccagaatg gtcgccggtt atcatacttg tatggatgtt ttgggattta
    50881 aagtaggaaa ttacttgttg ggtatttgct tttggaatat ctagcagacc taaaacagca
    50941 attaactgct gatctttaga aagataaatc acggttttgc cttgattttc caaacgttcg
    51001 atcatttccg caatctcttt gctgattatc atcgtctcag gatcatatgc gtgcttgcca
    51061 actttccatt gcgcacctaa ataggtagtt tgtaatccaa aaccagtaat ttcttctatt
    51121 ggtaagtatt ggatttcttc aggtattgtg caatcgaaat attgcaaaat cgcatgagca
    51181 agtggatgcg tcgttttttg ttccatagca acaagaagtt gttgtgccaa atccttttca
    51241 tcaaagaaaa agctgtcagt tacaactggt tttcctttag tgagcgttcc tgttttatca
    51301 aaggcaatcg cttttaattc agaaagcttt tctaaatgga taccgccttt gattaaaatc
    51361 ccattttttg cagaatttga aagagcagct agagaagctg gtgtagcaga tgcgacaagc
    51421 gcacaaggag aagccacaac taataatacc attcctcgat aaaagctttc atcccaactc
    51481 caacctagga aaaagtgggg gagaaggatc attagaggaa cagcaagcaa aatcaatttt
    51541 acataattat tttcgattcg ttcgataaat gaagctgttt gagttggcgt attctgcgct
    51601 tcttctacta attggataat cttagcaaag acagtttcgg aactcgtttt tgttactcga
    51661 atcgtgatgg gatttccttg attcagcgtt cctgcataca tctcatcgcc taaatatttt
    51721 tctacaggca cagattcacc gcttaatgca gactcatcga tcgttgcctg tgtgctttct
    51781 aaaaagccat ctatcggaac agctgctcct ttagcgacaa ataccaagtt atccaccttt
    51841 agttgagaaa catctacttc ttgcatttgt ccattttctt ttaataattg ggctttttgt
    51901 ggttgaagat tcatcaaagc agtaatttct ttttgacttt tattagttgt gtattcctct
    51961 aatgcaccgc ttaaacaaaa gatgaatgtc aacattgctc cttcaaagta attaccgatc
    52021 aaacaggcac caatagcagc taaagccatc aataaatcca cattcagtgt ccgatcattc
    52081 cacaattctt tcaagccttc cgatgtttgt ttatatccac cgatgataat ggcactgata
    52141 taaaaaagtg gggtgatggt aagagagaat cggtcgaaga taaaaccgaa gatcataaat
    52201 aaaaggcagc cgattgttgc tgcaaaagct tttttctgat tagtcatcgt tattagacct
    52261 ccttgtcaaa acaaggatag cataataaaa atgtaattga taataataac agctaaatga
    52321 gaacgataat ggttatcatt tggtgattaa gcttgatgtt gtttttatta tttataatga
    52381 ttattattaa agaaaataaa aaaccagaac atttctcaaa taagagaagt gttctggttt
    52441 tttctacatt gcaataaacc aataaccaat caaaatgata ccaaggataa tacgatacca
    52501 gccaaaagcg gtaaagtcgt ttttcttcaa atagcttagt aagaatttga tagctaagac
    52561 ggaaaccaca aaagatacga atgttcctac aagaagaaca acagtttctt ccattttgaa
    52621 tgaattccct tcagctaaat atttgacgat cttcaagccg cttgcgccaa acatgacagg
    52681 aatccctaag aaaaatgaaa actctgtagc gacaaaacgc gaagcaccaa tcaagataga
    52741 tcctaggatc gttgcgccag aacgggatgt tccgggaaca agtgccaaga cttggaataa
    52801 cccaacaaga agagctgctt ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt
    52861 gtttttgttg cgtttttcga caataataaa ggcaataccg taaacgatca aaacaatggc
    52921 aactggtaag aacttgtgga aatttgcttc taaccaatca tccaaaggca aaccaatgat
    52981 gatggctggt atgcaagcaa cgatcacttt aaaccaaagt gtccacgtat cttttttttc
    53041 taccgttgat ttacttggag aaaatgggtt taacttgtga aaataaagaa cgacgacagc
    53101 caagatcgcc cctaattgga taacgacatt gaacatcgac ataaattgtg aactggcatc
    53161 cagtttgatg aattcatctg ctaggatcaa atgaccagta ctactgatgg gcagccattc
    53221 cgttattcct tcaataatac cgagaatcac tgctttgata atatttgcaa aaaacataaa
    53281 ttccttcctt cccaattaag atacatctta tagtataccg ttattttttg agaaacccaa
    53341 tctgtttttt aatattaaga aaaacttgag aaataaagca gataagtaca cgaagagaaa
    53401 aaagaagaca aagccctcgt tcagactttg ccttttatga ttctctggta aaaattaaaa
    53461 tatccccatt atagaaacga gtcgcgacta tgtgttttcc cgttatcgat ataagacgta
    53521 tcattgatgc cgtggataga aaatcgaccg tttgcataag tgacctttgt tacgctgcca
    53581 tttaaaagcc cagcacgaac aggctgttta ggatcgatca gattaagcaa aaaggcgatc
    53641 gtcaaaccat gagatacgac taaaacattt ccaccaccat ttttttcgca gtgatgggcg
    53701 atatcttcaa atcctgtcca tacacggtct cgcaatgttt cataagtttc cgcccaccca
    53761 gcggtatcta agttgtatac agcattagca atttgttcaa aagaaacttc tgtggtaaac
    53821 atttcatcat atgttttaaa attcaataca cgagggatca caccccacat ttccatgtca
    53881 tatccacctt caagagaccc gaaacaccat tctctaatcc ttttttcaat aaaataagga
    53941 atttcttttc ccttagaatg ttctcctaga ataatacggg cagtttcgat tgcacgacca
    54001 ctgtcactag aataagcaaa tgaaaagtcg acatttttta acccgcgacc taaataatga
    54061 atgccttctt gtccttgctt tgtgaggggt gtatcacacc agccttgtac tcgttcaatt
    54121 gtattgaaca tcgtttttcc gtgtcgtacg atataaagtg tagtttctgt cattttattc
    54181 tcctcctaag tcgttttaac gattgaaaaa aggatttgtc cttttttcct gttcaatcgt
    54241 cgttgcatcg ccatgtcctg gatagactgc aaattcatca ggtaatacaa acagctgggt
    54301 ccgaatacta tgaagtaatt gttctaaatt ccctgtatac agatcggttc taccgatact
    54361 tcctctaaac agggcgtcgc cagaaacgac aaaatcatca aaaataaagc tgacgcttcc
    54421 tatagaatgc cctggggtag ggacgacgcg aaaagacatg tttcctaaag tataagtttt
    54481 catttcaaat tcatattctg caggcgagac aataacatca gcaatatcat catgtctacc
    54541 gagtccagaa agattcaaaa tcggatcacc tagccatttt tgctctagtg ggctgacata
    54601 aactgggatt tgataatacg cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt
    54661 atgggtaagc aaaatcgcta ccggctgttg gtttgttttt ttgatttctt gtttgattcg
    54721 ttccgcatct tctccagggt caatgatcaa aagtgcttct tcgttgtaga ccaaataaca
    54781 gttttcttca atcattcctg tttttaatct ttcgatatgc atgtgcattc ctccttgata
    54841 gccaaaactt ctttcatcca ttagtataac gcattcattg ctaggaaaca aaaacaggta
    54901 cttatttctc tttacttatc tgaattatca gactatatga aaagtatttg gatattctaa
    54961 agagaaggaa agtaagtttt catagcattt taatatgtcc ttgttacatt agattcatcc
    55021 aaaaatccaa attctatatc ctatatccct gcctaatcag cagggtgttt ttttatgttt
    55081 ggaaagagag ctctttttgc taaactaggt tttttcaggt aatcttagag agaaagaggt
    55141 ggtggaaatg atcaaacagt caaaaatatc tgtgcggcac ttagtagaat ttattttgcg
    55201 acgtggaagt atcgacaatc gtaaaaaaag taaccatact gcattagaag gagcgagaat
    55261 ccaccgcaag ctccaaaaag atgcgggaga aaactatgaa aaagaagtct ttttaaaaac
    55321 aacagttcaa ttagatgaat attacctgat cgttgaaggc cgtgcagacg gtattttcaa
    55381 aagagacggt atttattata tagatgaaat caaaacctcc gaaccacgat ttgaagattt
    55441 agaaccagaa caagtggaac ttttctttca tcaagcaagg gtgtatgcct atatctatag
    55501 tcaagaacat gatttacaag aaataaatct gcaattgact tacttccaga cggcagaaga
    55561 agtgatcacg agaaaggttg aacatcagac aagagagcag ttagaaactt tttttaaaaa
    55621 gctgactgaa gattataaag aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa
    55681 tgcctctttg atggctttga aattccccca tgatgagtat aggaaaggtc agcgtgaact
    55741 agcggtagct gcttataaaa cgatccgtac gaaacagaaa ttgtttgtag aagcacctac
    55801 cggaacagga aaaacaatct ctacgttatt ccccgctttg aaagctgtag gggaagaaga
    55861 gggagaaaag atcttttatc taacggctaa aacaataact agacaagtag ctgaagatgc
    55921 tatgacagca ttaaaagata caggagctga agtaaaaagt gtcactttga cggcgaaaga
    55981 caagatctgc tttttagatg agacgacctg taatccagat cagtgcccat atgccaatgg
    56041 ttattacaat cggattaatg aaggtttatg ggatcttttg aatcatgaga atcagatcac
    56101 tcgtgaagtc atcgaaactt atgcaagaaa gcatacactt tgtcctttcg aactatcatt
    56161 agatgtcagt atctggtgtg acgtaatcat tggggattac aattatttat ttgatccaac
    56221 tgtttattta agacgttttt ttgaagatga aaaaaacgag gactatctgt ttttgattga
    56281 cgaagcacat aatttggtga atcgttcccg agagatgtat tcggcagaac tatcttatga
    56341 aaaaacgaaa cgaagtaagg aagctatacc aaaggaattc aaaaaacttc atcgccgttt
    56401 caacaaacta ttaaaagaat ttgattcgat ccgagaaata gcaaaagaag atcattggga
    56461 ttatcatcat caaaaagcac cggctgaatc tttagtaaag gcaggttatc aattaagcga
    56521 aaaaattaaa gaatggctgg cagaatttcc tgagcaccca tcacaagaac aattgcttcc
    56581 ttattatttt gatttgcttc attttttgaa ggtcagtgaa ttttatgatg atcactacga
    56641 aacaacagtc gaaaaaacgt atcgcgatct gatagtaaaa gagttttgta ttgatccaag
    56701 cttgtttctt gagcagtcgt tggataaagg aaaaagcagt attctttttt ctgctagttt
    56761 ttcaccactc tcttattatc aggaaacact ggggggacaa aaaagtctgg cttacaaact
    56821 gccgagtcct ttcccagaag gaaaccagca agttttcatc gcaaattacc tagagacgac
    56881 ttatcgaaaa agggaacaca gtctggcaaa attagtagag acgattcatg cttttgctga
    56941 tggaaaggtc gggaattatt tcgttttctt tccttcttat caatatttag atcttgcggt
    57001 ggaagcattc agacagcaat atcccgacag caatgtgcta atccaagaaa cggacatgaa
    57061 tgaggaagaa cgcgaacgat ttttaacaaa atttcaaaca aatcccgctg aaacattgat
    57121 tggattttgc gtacttggcg ggattttttc agaaggaatc gatttgagag gggaccgatt
    57181 gatcggcagt atgatcgtag gtgtaggttt gccgcagatg aatcatgagc aggaactgat
    57241 caaatcatat tatgatgaga aggaacacct tggatttgct tacgcttatc agcttcctgg
    57301 aatgaacaaa gtcttgcaag ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat
    57361 cgttgtattg gcagatcaac gattttctag tttttcttat cgacgtttat tcccacaaca
    57421 ttggcaaaca gcaagagaag tacgaagtgt tggggaattg gaagaaggaa tacagcgttt
    57481 ttggttatca aaagaagcag taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt
    57541 ttttgattaa cgatagttag tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa
    57601 agcaatcaca gcttgtagat catcacggtt cttacctgtc actctaatct gatcttcttg
    57661 gatttgtgct tttactttca gtttttcatt tttgatagct gtagtgattt tcttcgcgtt
    57721 ttcacgatcg attccgttga ccaaatcggc agtttgtcga gctttgccac ctagagcgtg
    57781 ttctgattcg gaaaaatgga tatttttgat tggcacattt cttttgatca gcttaccaaa
    57841 taatacatct ttgatctgtt cgattttgaa atcatcatcg ctcaatacaa ctagattgct
    57901 gttctctaat ttgatttcta ctgtagaatc cttgaagtca aagcgattgg acaattcttt
    57961 tgttgtttgc tgaatcgcat tttttacttc ttctgtattg acttctgata caatgtcgaa
    58021 gctagcatct tttgccatag gaaccctcct gatatacata taaatattta ccatacatct
    58081 atcttatcat aggattaggg cagattctta cctgtttgct aaaattcttc tcatttgagt
    58141 tcgaagtatt ttattttaat agataaactg ttattcttac atttggaata ccaaagtagg
    58201 ggagaggtgc ttgtgagtaa aattatgact gcgtatcata aaaatgaaag attaaagaaa
    58261 atagtggtta ttttgataac cggattgacc gctgcggtcg ctttaaatta ttttttgatt
    58321 ccagccaatg tcttttctgc agggatgaat gggatcgctc aaatcatttc ctcgctttta
    58381 tatgaatggt ttcatatcaa gatagacaca ggaacattta tttttctttt gaatatccct
    58441 gtgtttgtat tagggtttct caaagtagga aaacgagcaa cgatccttag ttttgtgaat
    58501 gttgtatgtg tatcggtgat gacgatgctg cttccagtcg gacagatcac tgataatatt
    58561 ttgatgaatg ccctagtagg cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa
    58621 atgggcttta caactggtgg gatggatatc atctctcttg tactttcaca aacaactgga
    58681 aaaacagtcg gaaactacat gctgattcta aatggtatca ttgtagtcat cgcaggcttc
    58741 ttctttaact gggaaagtgc cttgtatacg attatttcca tttatgcaat gactcaagta
    58801 attgatgcca tccacacgag tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca
    58861 gaagaggtaa cagaagaact agcaaaacgg atggtacgtg gaatgacatt actgccgtct
    58921 gtcggtggct attcaaaaaa acaagggaac atgatcatga tggtcatcac ccgctacgaa
    58981 atgtatgatc ttgatcagat cgttcatgaa atcgacgacg atgcatttat caatatttta
    59041 ccaactcatt cagtatttgg ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca
    59101 actggcgtat tgccagaaat aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt
    59161 ttagaataaa tttaatctat tacatctaat aagaacattt ttataaattt aaacgagaat
    59221 cttacttcta aattctcatt tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt
    59281 gttaaggagt gtcgtacgat gagcgaagca aaccaagaac ggtctggaca agaaatagaa
    59341 ctaatcaaag aacgtattct tgctgccttg gaaatggtga ttgatcctga attaggaatc
    59401 gacatcgtca atttaggctt gatttatgat atagaattca atcctgaaaa tggagaaaca
    59461 gtcattaaga tgactctaac aactatgggc tgtccattag cagatatttt aacagaatct
    59521 atccatgacg cattaaaaga agtgccagaa gtaacgaaag cagaagtcaa actagtctgg
    59581 tatccagcat ggacgacaga taagatgagc cgttatgcac ggattgcgtt ggggatacga
    59641 tagaaatctt ggctgactag ctatgagaag caaataggaa aaacaaaaaa gccttttatc
    59701 ctttctagaa gaaaagagat aaaaggtttc tttgtttata aaattttgtc tgctatatta
    59761 taagttaatt aaataaatgt gttctattga taaaggacat tcaaatccct ttccttttgg
    59821 aatccttctt ttttacatca gtttgatttt cgtatagtat tctttctcat cttcacgata
    59881 aatcgcagcc ccattttttt gttgtgcttt aagactgcca agattttttt tatttacaga
    59941 caaaagtgcc tcatcaatgc cgattttttt tgcctcttgt agtactaatg ctaatccttg
    60001 tgtggcatat ccttttttac gataattagg ggagatgccg taaccaatat ggccagcgcc
    60061 gtttcttaat gtttcgttca gtttatggcg caaattaaaa attcctacat actcatgttc
    60121 atcactttct aaaataaaga cggttgcggg aacgtaactc tcaggaagat cgattcctaa
    60181 agacgttttt tcgcattttt ttacataatc taaaaattca gcaaatgtat agccataagc
    60241 attgttgata aatccactct catttggtcc aaagctagtt tgtagctgat aagctttctt
    60301 taaatctgct tcccatagtt tgatcagttt catcgtattc acctctttaa gtaaccttat
    60361 tcatttgttt tctactatag cactattgtt gtaaatagcc atgcctattt ttatttattg
    60421 tgtcatatcg tattaagaat catcgaacaa tacaaatatg aattacaata aaaaaattat
    60481 ttgaagttcg ttcattcgtt acttatgatc tctagaagaa agaaaacgtg aattacttca
    60541 gcgtctagaa ttccagaagg atagtaatca acagaagaaa taatcaatat ccaaatgaaa
    60601 gaagatagct gaatcgagaa aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta
    60661 ctaatcccag agtcgttcgt ttaacattga aatcgatcat atggataaaa cagatttttt
    60721 caccaaccct caatagatga tggagttgct cggatgaatt atttgtgtag atggcataag
    60781 ggagggcgta cagaaatgaa aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat
    60841 cctaaactaa aagatgtgat gcggctatgg cggaattggc agacgcgcta gcttcaggcg
    60901 ctagtgaggg tgacctcgtg gaagttcgag tcttcttagc cgcataattt gtttttttat
    60961 aaagagaagg tacacctgga gcgatgagaa agaaaataac atattatcta aaagaatata
    61021 ggaaaaattt taaacaacgg aaccagattc aaataaatca aaaaaaattt gtgtgaaggt
    61081 gggacagttt aaaacctaat agattgattc aaatagtaga tttatagatt ctgatacaag
    61141 aaatactggc aaacgatagt taaggaataa agactaaact taaactcaaa aagaggtcat
    61201 gcgaaagaat aaatcgcatg acctcttact atattttgaa tgtgtttaaa cgttcaaata
    61261 atgaatctta gtaccaaccg tttgcttccc agaatgcttt cgccgcttcc caagaaccat
    61321 aacgtgaagt aacatattgt tctgctactc tttcttggtt tgcagcagag tagtcaccat
    61381 ttaagtaaga agaatctaat tggtaacggc cgatgtaacg tccgttcgtt gctgtgtaag
    61441 aaccgcttga ttctttttgt gcgatccatt cttttgcaga gcttgtactt gctggagtag
    61501 cagcagtttc tgttacagca ggagcaggtt gtgtttcagc aacaggcgct gcttgtgttt
    61561 ccgtttgaac cgtttcttgt acaacaggtt gttcagcttg aacaggttca gcttggactg
    61621 gtgcttcttg aacggctgct tgaggttcag cagctgcttg tgcagagcct tctgttggga
    61681 ttgttaattg ttgtcctgag ta
//
LOCUS       DACCYH010000006        59388 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.006, whole genome shotgun sequence.
ACCESSION   DACCYH010000006 DACCYH010000000
VERSION     DACCYH010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.006"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..230
                     /locus_tag="IVY85_000358"
     CDS             <1..230
                     /locus_tag="IVY85_000358"
                     /inference="COORDINATES: protein motif:HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043398.1"
                     /translation="YQLVETQAPIGYELDATPIEFEIERSQTAVVELTKENRLTPGGV
                     VLTKIDDQSGEILQELFLSYRTEKEKRYKPV"
     gene            176..559
                     /locus_tag="IVY85_000359"
                     /pseudo
     CDS             176..559
                     /locus_tag="IVY85_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(983..1153)
                     /locus_tag="IVY85_000360"
     CDS             complement(983..1153)
                     /locus_tag="IVY85_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8043399.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1347..1526)
                     /locus_tag="IVY85_000361"
     CDS             complement(1347..1526)
                     /locus_tag="IVY85_000361"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043400.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            1913..3025
                     /locus_tag="IVY85_000362"
     CDS             1913..3025
                     /locus_tag="IVY85_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8043401.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3103..3396)
                     /locus_tag="IVY85_000363"
     CDS             complement(3103..3396)
                     /locus_tag="IVY85_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP8043402.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3564..3878
                     /locus_tag="IVY85_000364"
     CDS             3564..3878
                     /locus_tag="IVY85_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP8043403.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3899..5878
                     /locus_tag="IVY85_000365"
     CDS             3899..5878
                     /locus_tag="IVY85_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8043404.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5891..6217
                     /locus_tag="IVY85_000366"
     CDS             5891..6217
                     /locus_tag="IVY85_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP8043405.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6233..6751
                     /locus_tag="IVY85_000367"
     CDS             6233..6751
                     /locus_tag="IVY85_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043406.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6751..8103
                     /gene="celB"
                     /locus_tag="IVY85_000368"
     CDS             6751..8103
                     /gene="celB"
                     /locus_tag="IVY85_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8043407.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8187..9692)
                     /locus_tag="IVY85_000369"
     CDS             complement(8187..9692)
                     /locus_tag="IVY85_000369"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP8043408.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9784..10374)
                     /locus_tag="IVY85_000370"
     CDS             complement(9784..10374)
                     /locus_tag="IVY85_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8043409.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10553..12199
                     /locus_tag="IVY85_000371"
     CDS             10553..12199
                     /locus_tag="IVY85_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8043410.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12248..12967
                     /locus_tag="IVY85_000372"
     CDS             12248..12967
                     /locus_tag="IVY85_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8043411.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13446..14393
                     /locus_tag="IVY85_000373"
     CDS             13446..14393
                     /locus_tag="IVY85_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8043412.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14405..15325
                     /locus_tag="IVY85_000374"
     CDS             14405..15325
                     /locus_tag="IVY85_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8043413.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15366..16823
                     /locus_tag="IVY85_000375"
     CDS             15366..16823
                     /locus_tag="IVY85_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8043414.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17168..17782
                     /locus_tag="IVY85_000376"
     CDS             17168..17782
                     /locus_tag="IVY85_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8043415.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17775..19499
                     /locus_tag="IVY85_000377"
     CDS             17775..19499
                     /locus_tag="IVY85_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8043416.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19496..20947
                     /locus_tag="IVY85_000378"
     CDS             19496..20947
                     /locus_tag="IVY85_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8043417.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21420..23573)
                     /locus_tag="IVY85_000379"
     CDS             complement(21420..23573)
                     /locus_tag="IVY85_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8043418.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23575..24621)
                     /locus_tag="IVY85_000380"
     CDS             complement(23575..24621)
                     /locus_tag="IVY85_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8043419.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            24771..26060
                     /locus_tag="IVY85_000381"
     CDS             24771..26060
                     /locus_tag="IVY85_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP8043420.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26073..28775
                     /locus_tag="IVY85_000382"
     CDS             26073..28775
                     /locus_tag="IVY85_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8043421.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            28760..29617
                     /locus_tag="IVY85_000383"
     CDS             28760..29617
                     /locus_tag="IVY85_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8043422.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(29688..30545)
                     /locus_tag="IVY85_000384"
     CDS             complement(29688..30545)
                     /locus_tag="IVY85_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8043423.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            30756..32927
                     /locus_tag="IVY85_000385"
     CDS             30756..32927
                     /locus_tag="IVY85_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8043424.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            32995..33336
                     /locus_tag="IVY85_000386"
     CDS             32995..33336
                     /locus_tag="IVY85_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8043425.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33612..35366)
                     /locus_tag="IVY85_000387"
     CDS             complement(33612..35366)
                     /locus_tag="IVY85_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8043426.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35377..36213)
                     /locus_tag="IVY85_000388"
     CDS             complement(35377..36213)
                     /locus_tag="IVY85_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8043427.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36215..36898)
                     /locus_tag="IVY85_000389"
     CDS             complement(36215..36898)
                     /locus_tag="IVY85_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP8043428.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            complement(37057..38451)
                     /gene="uxaC"
                     /locus_tag="IVY85_000390"
     CDS             complement(37057..38451)
                     /gene="uxaC"
                     /locus_tag="IVY85_000390"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8043429.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            complement(38490..39989)
                     /locus_tag="IVY85_000391"
     CDS             complement(38490..39989)
                     /locus_tag="IVY85_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP8043430.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            complement(39982..41403)
                     /locus_tag="IVY85_000392"
     CDS             complement(39982..41403)
                     /locus_tag="IVY85_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP8043431.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            complement(41463..42455)
                     /locus_tag="IVY85_000393"
     CDS             complement(41463..42455)
                     /locus_tag="IVY85_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8043432.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            42687..44885
                     /locus_tag="IVY85_000394"
     CDS             42687..44885
                     /locus_tag="IVY85_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8043433.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            complement(45343..46215)
                     /locus_tag="IVY85_000395"
     CDS             complement(45343..46215)
                     /locus_tag="IVY85_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8043434.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            46515..47489
                     /locus_tag="IVY85_000396"
     CDS             46515..47489
                     /locus_tag="IVY85_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8043435.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            47511..48203
                     /locus_tag="IVY85_000397"
     CDS             47511..48203
                     /locus_tag="IVY85_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8043436.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            48169..49677
                     /locus_tag="IVY85_000398"
     CDS             48169..49677
                     /locus_tag="IVY85_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8043437.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            49751..50854
                     /locus_tag="IVY85_000399"
     CDS             49751..50854
                     /locus_tag="IVY85_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8043438.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            50857..52194
                     /locus_tag="IVY85_000400"
     CDS             50857..52194
                     /locus_tag="IVY85_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8043439.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            52181..53059
                     /locus_tag="IVY85_000401"
     CDS             52181..53059
                     /locus_tag="IVY85_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8043440.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            53056..53979
                     /locus_tag="IVY85_000402"
     CDS             53056..53979
                     /locus_tag="IVY85_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP8043441.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            53976..54386
                     /locus_tag="IVY85_000403"
     CDS             53976..54386
                     /locus_tag="IVY85_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP8043442.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            54390..55958
                     /locus_tag="IVY85_000404"
     CDS             54390..55958
                     /locus_tag="IVY85_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP8043443.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            56198..57616
                     /locus_tag="IVY85_000405"
     CDS             56198..57616
                     /locus_tag="IVY85_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8043444.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            57628..58938
                     /locus_tag="IVY85_000406"
     CDS             57628..58938
                     /locus_tag="IVY85_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8043445.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
BASE COUNT        19129 a        10376 c        12108 g        17775 t
ORIGIN      
        1 catatcagct ggtagaaact caagcgccaa tagggtatga attagatgcg acaccaatcg
       61 agtttgagat cgaaagaagt cagacagcgg tggtagaatt aacgaaagaa aaccgactta
      121 caccaggcgg tgtcgtgttg acaaagatcg atgaccaaag cggggaaatc ctgcaggagc
      181 tgtttttgag ttacagaacc gagaaggaga aacgctacaa accggtctaa ctacaggaga
      241 agatggaaaa ctggcaattg acggtttagc accaggagca tatcagctgg tagaaactca
      301 agcgccaata gggtatgaat tggatgcgac accagtaact ttcaagatcg aaaaagaaca
      361 agaagtggcg atctttttaa caaaagagaa tagaaaagtt gctgatagct cagatataag
      421 aggaaacaat ccaacaagca gtggaaataa gcatcttcca aaaactggag aaaccatcat
      481 tagccagttt atattgtcaa tattgggtgt gctacttgtt ttcatcagta taaaatttag
      541 aaagaaacga aatatatgag ctaaaataaa aaattgaata agcatagttt ttaaaatcaa
      601 ctagtaccca tcaattgaat ggaaactacc gattttagaa ggggtataca aacaaatatt
      661 agtatcggag tagagaaggt tgaacccaac cagtaaataa gtaaagacaa ttttgtgctg
      721 cgttgagaag tgaaaaataa acctttgtag taatactagt taataaaaaa tatacaaaaa
      781 cactgcgaaa atcattcttg agtttcgcag tgttttttgc ctaatgactc aagtttattg
      841 ggtaatactt tgattttctt tctacaaata atcagcgatc agttgtaatt ttttctctag
      901 ttttaatggc tctttgtcaa taaggagaaa aacaagtcgg gtcttttgtc tcggaaagct
      961 tcaaccaact cgtgaataaa ggttacgcct ccttgaaagg cgtggaaaaa ccaagtaatc
     1021 gatcaatcac caatttcgca tttggtagaa catgtttggt gagctgaaag taggtggcgt
     1081 tcatgtctgt aactaagaat tctatttctt taggattagt gcagctaagg aaatagtttg
     1141 ttaatcgagg aattttttgt gtaggtaaat tttctcgatt aatttttctg tctcgccatc
     1201 cgcggaaata aaactcagtt tatcctcaac ggaaacgtga gaacgaaatt cacaaccctc
     1261 aacactcaag acagaattct tttggaagac tttggtaaat agcttctcaa ctctttcaat
     1321 gtgcgaataa cggttgtcaa agatacctaa caacttttcg aaataaaaaa taacgacact
     1381 ttttcagtca gtaaagaagt gattttatgt ctgacatgat ttgaaatcga gtgccttgga
     1441 cggataaaat aactttgagc gttccaatgg cttcgacagt ttttacaggt atagcgctgc
     1501 ttttttaggc gtataaccat tggcatatga ttatattatt caaagtggac aatggtttcc
     1561 ttcttcttgt ttttcaccac cattaatttc ctatttccat ctaccacagc agaaccacaa
     1621 tttctacaga cacatggagc agggaagaga actgcagcaa cgatcaatgt ttttttcttc
     1681 tgtaaggttt cgtaagagac ctctgtaatc atcaaatttt tgtctattga tctcagaatt
     1741 tttttgatag aaccattcat aaagcatatc gttctctcag ttgtttattt tgtggtgatt
     1801 taatcataca agaaaacgat atgttttttt ataaaaacat gaataggaaa aatagttgac
     1861 aagaaaatga caacgccata tgatgttcca tagtatttac aacctgtcat agatgactgt
     1921 aaaaaataaa agtaaaaaga aaggacgaca acaggtggat ttttatcagt ctctacaact
     1981 agatccgttt attttaaaac aaaaaatacg agaagcagct tctaaaaaag aaaagtgcat
     2041 gtatatttgt tcattgtttt tacgatcatt attcatcgta ttgtttgcca tttgttttat
     2101 cattataatt acaacattat ttgaatcgaa gcataaacca tacgctgtgg ttttattctg
     2161 tatgttgatg agtattcgtt ttgttgactt tggttataag atttcccatt cgatcatagg
     2221 tttagcaatc gtcatgttct cactattagt agcaccgtat gtccagctga tcaaatggtc
     2281 agttatgggc atgttgattc attttatttt actaagcagc atattaatgg caaccgctag
     2341 tgatcccaaa atggggaatg cttctttgta cggcttttca tatctcttta ttgtttattc
     2401 gttacctaaa gattcgttga acaagaattt ctttacacaa acaagtagct tattattttt
     2461 gtttttttgt tggttttcag ttctccttta cagaaaacat cgagaaaaga acaaagacaa
     2521 aagcttgttt aaagagattt ttttgaaagg catgtattcg caggaaaaaa tttggatgct
     2581 catctatgca tttggcatta gtcttttgat cgttgcagga gagtatgtcc cttttcagcg
     2641 attgatgtgg gcagggtttg cattttcttc tattgtatct agctatggat tgatgtcgat
     2701 tggtttcaaa gaacgagcag tagaccgaat cattggctca ttgattggat gtgttttatt
     2761 tattggtatc agccaattta ttccttttaa ctgggtcggt atattaggtg gtctagcttt
     2821 aggtgtttgt tctacctata gatataaaac agtttttaat tcttttggtg ctttggcaat
     2881 cactgccagt ttattcgggg ttccaggagc agtaacgatc cgcatttttg agaatatcct
     2941 tggggtatgt ttaggaatca tgtatattgg ggtgactgaa atacttatcc gaaaaataag
     3001 agaaaaacac ggtttgaatc attagatgag atcgaataaa aaataaggaa aaagtgaaga
     3061 tacacatgga tgataaattg ctatcattcc atgtgtatct ttttacggta aatcagtgaa
     3121 acgttcgact ttcattttta atttatactt tttacggagt tcgtttattt gagccatcat
     3181 ttcggatttg tgatgctttt cgattgcttc ttcatctttc caacggtcga tcagtaaaag
     3241 agattctgga tcttctagtg gaaagtaata gtcatatttc tcgtttcctt cttctgcacg
     3301 aatcgctttg acaatgtttc tttcgaccat ttcttctgca aattttctgg cttgtccatt
     3361 tgtacttgta tacaaaatat tcactgtgat actcactaaa tcccctcctg aaaaaattct
     3421 atcactagta tatcacggtg ccagaaatta ttattcaaga tatgatacac attttttccg
     3481 caatgctacg gaaatatata ttttgaatag aaaacgctta caagatatgc ttggtttata
     3541 aaaataaaag gagtgattga tttatgaaaa aagcattgat tatttgtgcg gcaggaatgt
     3601 catcctcgat gatggcctca aagacaacgg atttctttaa gcaaaaaaga gaagaggtac
     3661 tcgtagatgc agtttcagct acagaaggcg ataatatgat taagaatagt gattttgatt
     3721 tatttctgat cagcccgcaa acgacgatgt ttttggataa attcaaaaaa ttaggagaat
     3781 ctgttggaaa gcctgttgtt agtatccctt tccaagcgta tgtcccaatc cctacaggca
     3841 tccagaagtt agcagaggtg atcgaagaga acatatgatg gaataggagt ggatgataat
     3901 gaaacagaag gaaaaattgc tgttgagata tttgattgat cacaagaagg agtatgtcac
     3961 cagtcaacgt ttagcttctg aattgttatt atctgatcgg acaatccgta attacttgca
     4021 gcggatcaaa gagttagtag aaaaaaacgg aggtaagatc attgctaaac caggttatgg
     4081 ctaccaactg catattttac aaagactgac atttgacttg tttttgagtc ggcaagaaat
     4141 agtacctgga tatgctagag aaccgcaagg attttatgag tccgaagatc gacaaaagta
     4201 tatattgaac caattgcttt tggaagatgc cattcttttt atggatgatt tggcagaaga
     4261 gctttatatc agtcgttcca gtttgacaaa ggacatgcaa gaaatcaagg agcgattgat
     4321 tccatattca ctaaaaatcg tttctaagca tggtcaaggg acatggatcg aaggggaaga
     4381 aagaaatcga cggcatttta tcatggatac tttttttgga aaccattatg gaaattcatt
     4441 gaaagaatat ttagggcaaa gccgtttttt cccagacatc agttttgaag agctagtcat
     4501 tatcattctc gatgaaacaa gagaagctaa gttaaaagtt tcggatttta tcatacagaa
     4561 tctgacttta cacttggctt tggggatcaa acggctccga gaagggtttg aaatcaagga
     4621 attaggcatc gaacaagaag tttctaagcg agtagagtat caagtggcgc agcggatcgt
     4681 tcaacgtatt gaggtaatcg caggtgtatc gtttcccgaa gaagaaattg cttatttgac
     4741 attacatctc atggctaagt cgaatcacaa agaaaatcaa aatgatcagg aactatcaaa
     4801 ggaattagcg aaagttctga aaagattgtc tcaggaattt catttttcat tagtagagga
     4861 tctccagctt ttcaatggac tactcgatca tttgtcaccg atgatgattc gattgagccg
     4921 aggtattcgc attgagaacc cattgactga ggagataaag aaagaaaatc ccaaagtctt
     4981 tgacgcagta aaaaggcatt tcagcaacat gccggctttg aaaaactata ccatcaatga
     5041 agatgaatgg gcctatttgg ctttgcatct catggcggca ttggaaaaag aaagagctgc
     5101 gcataaacta catgcattga ttatctgtgc aacgggttac ggcagtgccc aactattaaa
     5161 gaatcgcgta gtcagtgaat tcggtaaaaa tattacagtg gtaagtgtaa aaggctatta
     5221 tgagatcaat gaaacaactt taaaaggaat cgacctgatt atttcttcta ttgatctttc
     5281 gacaatgttt ttcaaaattc cagttttaca tgtcagtatt tttttgaatg aagatgatgt
     5341 tcgtaaaata cgaaaggtag ttggagaatc tataccctat cattcctcag atagaccgat
     5401 accagcattt tctctacagc aaaaaaagca gatttacacc gaacagttgt ctgaaaaatt
     5461 tttcaagact tatacgtacg aaccgaaaaa ggaagaaatt cttcatgatt tgctgcaaaa
     5521 gctgagcata aatgaagaag aaggatatat aagagaaatg cgaaagcaga tccagcaacg
     5581 tgaaaatatg ggacaaatca tattcagtga tacagtggca gtcccacatc ccgctgttcc
     5641 agttggaatc gaaacaaagg tagccgttgc attgattcct tctgggatgc agtgggatca
     5701 atatcaagaa attcattttg tttttttagt ttcgccttct tatgtcgaaa atgaaggaat
     5761 tacggtcgtg actaaagcaa tcgttaggtt agttgaccag ctaaaagtac agcaggaaat
     5821 cttagctgaa cctatttttg agaatttcag cagtaaattt atcaaactga tcgaataagg
     5881 aggaaaacag atggctgaaa aaataaacag tgaaacacta caagtgactt cttttgatat
     5941 cattttccat agcggaaatg cacggacact gatacatgaa gcatttcgct cgatgcgtaa
     6001 agagatgttt gaagaagtag aagagaagtt gcgacaagca aatgaagaga ttcttcatgc
     6061 gcatcagtca caaactcaac ttctgaaaga atatgccagc gggacaaaaa tagaaatgga
     6121 aattatcatg gtccatgcac aagatcatct gatgacgacg atgacattat tggaagttgc
     6181 acaggaaatg aattatttgt atcaaaaaaa taaatagaaa ggaaggatca ttgtgcagga
     6241 tacagtaaaa aggaaaatcg aattagaaaa aaagcaactt agtttgaaaa acatgtattt
     6301 caatcgttat ctaatcgtac gttacctgac tgcctttttc tttttcatga acatgcaatg
     6361 gatgattctg cttctatcag ctaaaagttt aggaagtagt ttaccgatga ttctgatttt
     6421 agctattttg cctgcagtag gagaacaagt aaagctttat cgaaaacacc aaacgaacgt
     6481 tccatggaca aagtggtatt ttttgttcca gagtgtcttt aatttattag tgattccagt
     6541 gttgtttacc tcaggattta cattcttgta tccttttatg gcaaataaca atggaggaca
     6601 gttatttgtt ttcattcttg ttgtaagcgg tatttttgcc agtgcattta tacagtatcg
     6661 tttaaagaaa atttcactca accaagacca acagtatatc cgtattaaac aatatgaaaa
     6721 agcgttatat ttaggaaagg agaataacta atgtttgatt ttttgcagaa atacctgatg
     6781 ggacctatgg ggaaaattgc gcaacttaaa atcgttcggg ctgtcatggc agcggggatg
     6841 gcttctattc ctttcacgat tgtaggctcg atgtttttgg ttttaaatat tttgccatta
     6901 ccgttcccat ttttagaagg attttttaat gcaacttttt tcaaagttag tgacttgtac
     6961 atgatcgtca atacaatgac gatggggatt ttatctattt actttgccat tgtgtttgct
     7021 tatgaattaa cgtcaattga acgagatgaa caaaaattaa atgttaatcc gctcacgggg
     7081 gcattactat ctgtttttgc attctttatg tgtatccctg aattgatcat gactgatggt
     7141 aaaatcagct tgattgcttc tatcaatgag acagaaactg tagtaaatgg tattaggatg
     7201 ggctcttttg tcgaacgttt aggaacttca ggaatcttta cagctattat tatgtcgatt
     7261 attgcggtcg aattgtattg tatgtgtgtc aagagaaatt gggtcataaa aatgccagat
     7321 gttgttccgc caggagtttc tcgttctttc actgctctga tcccaacatt tgtcattgcg
     7381 tttgtggtga tgatcatcaa tggattatta gttgcactag gaacggatat cttcaaaatg
     7441 attgctatcc catttgggtt tgttacgcag ttgacgaata catggcttgg tgtcatggtg
     7501 atttatttct tgatccatgc attgtggatc gtagggatcc atggagcgaa tatcatcact
     7561 tctttcttga ccccgatcgt attagctaat atggcagcta acgcacaagg cgcaaattat
     7621 ccgttggcag gagaattcaa caattcttat gtaaccgttg gtggatcagg tgcgacttta
     7681 ggattgatca tctttattgc attcctcgcg aaatctgatc agctgaaagt gttaggaaaa
     7741 gcctcacttg taccaggatt attcaatatc aatgaaccaa tcattttcgg gatgccaatc
     7801 gtctacaatc cctatttagc ggttccgttt ttcttagctc cgatggcttc tgctacactc
     7861 gcttattttg ccattcggct ggaaatcgtc aaaccgatgc ttgcacagat gccgtggcct
     7921 tcaccagtcg ggatcggagc atttgttggt agtggtggtg attggaaagc tgctgtgtta
     7981 gcagtcattt gtgcaatcat cgcattcatt atctggttcc catttatcaa attctatgat
     8041 aataaattat taaatgaaga aagagaaaag gcagcagaat tagctagtga aaagttaaaa
     8101 tgatttctaa gaaaaaagga actgtgacaa aaaatcacag tccctttttt tcttagaaat
     8161 gggaaaccac tagtatataa ataccattat tcttcaatca accgcttcac gattgcataa
     8221 tccccttcat aaggttcatc tacaccattg attttttgat agggatccac gccttttccc
     8281 gcaataacga ctgaatcttg cggagcgctt tctgctaaag cgcgtcgaat cgcttcagga
     8341 cgatccattt cataatgaac gatcaaatct ggattagtaa ttgcttcatt gatttcttcc
     8401 gcgatttttt tcggatcttc aaatgctggg tcatctgccg tcaaaaaggc gatgtcagct
     8461 gtttctgata gcacttttcc aaaatcatgt cgacgggaga ttgctttgtt tcctgggctg
     8521 cctaatacaa caattacacg gccatctgga tgttcatttt tggcaaattc cagcaatgtt
     8581 ttcaagctta gataattatg tgcatagtcc acatagacat gcgctccatt ggattggatc
     8641 aattgatcca ttcggcctgg tacacgagct tcttttaatc cgtcttgtgc atcttgactg
     8701 gaggcacctg ttagtgctgc ggcaatgatc gcactggctg cattttcttg gttgaatcct
     8761 ccagccagca aaatatcata agcagtatcg cagatagcta agcgatcttc atgagaagat
     8821 aaagtaaaac cgtgagtacc tttatctgaa cggatcactt gataattgct ttcttccgaa
     8881 cgaccataag tgatcgttgg aatctggaat agatcggctg tttccctaag taaatcaaaa
     8941 tagtcacttt catgatttaa aacaactgtt tttgaattca agatcaattg tcgcttgcaa
     9001 tagaaatagt catcaaatgt cggatgttcg atcggactga tatggtcagg cgagatattc
     9061 aagaagattc cgacatctaa agtcaagccg tatacgcgtt gtgttttgta agcttgtgaa
     9121 gaaacttcca tcactagatg agtcatacca ttttcaactg cttctgacat catacgatat
     9181 aaatctaatg actcaggcgt tgtcaaatgg gatttgaaat atgttttgcc atctaacgtt
     9241 gtattcatag tagaaagcaa tgctgttttt ttatttgttg tgtgatccaa gattgctttt
     9301 gtaaagtacg cagcagttgt tttacctttt gttccagtga atccgatcac tttcaattta
     9361 ttttgcggat agtcgtaaaa agccatagca agaagcgcca ttgctttacg gatatccgtt
     9421 acgatgattc ctactgctga attttcgtat ggttgttctg atacataata ttgaataccc
     9481 tgttcgattg ctgaatccaa atattcttct ttgaaattca ttcctttaca gaaaaacaaa
     9541 gtagaagcat cagcttttct tgaatcataa cttaactgaa gaaattcagc tttttctggt
     9601 acatcaagat gccagcctct tgttgaaaca aattctttta atagattttc ttttaatagt
     9661 aaatgacgga tgtcttctaa agagaagctc attgattgat ccctcgcttt attcaatttc
     9721 ttggtttatt atagcacact ctttttaata tatcgtttgt ttccgctatg acaaaatcca
     9781 gctttattca aattttcttg acacagggga caagcggatg agtattttca taaacaatga
     9841 actgactgta atagatagga gaacgaatag tgtctccaac aatccaaaaa cataataatt
     9901 caaaaagacg ataagactat tggtaagtaa aagtccttgc ggcattggaa gccgaaaaaa
     9961 tttgtggaaa ataatcggcg gtatcgttat accgccattt gaggccccga ctgtgtacaa
    10021 tgtgtaaacc ccaagactga acaaaagact tcctgaaatc agtgaaaaga aatgtgagga
    10081 gatcatttcc acttttggaa caatctctaa aacaacagga aaaagcagac ttcccgctac
    10141 agtcttaaaa aatactgatt ttcctaatag gacagcggct agaagcagca tcataagatt
    10201 gatgctccaa acagtaactg agcgttggat agaaaaggcc gtttcaacta aatgtccgat
    10261 ggcgcccact ccggcggaag ctatatcgtg agggagcaag aaccaattca atccaactgt
    10321 gataaagaaa agacctaaaa atataacggg tagttttttt atttcttgtg tcattgatat
    10381 ctcctataaa tgttagccat aaaaggctgt atatttcttt actttaccaa aaaaacgaaa
    10441 ccgaattcaa aaaaacaatt gttcttataa aagttatata aaatcgattg ccagtttctg
    10501 ttttaccaat taaaatgata gtaatgtttt gtagaaagtt ggaatctgac cgatggataa
    10561 tcaaagaaaa ccagcattag atcaattaac accgcaagaa aaaatggcac gaggctctgc
    10621 ttggatgacc gccagtaata tcatttcaag attgctaggc gctgtctata tcattccctg
    10681 gtatgcttgg atgggcgaaa atgctaaggc tgccaatgga ttattcaata tgggatacaa
    10741 tatatatgca ttgtttctac tgatctctac agcaggaata cctgctgcga ttgcaaaaca
    10801 aactgcacgc tataattcat tgaatgaata tggcacttca cgacgattat ttatccgtgc
    10861 attacagatg atgggcgggc taggcctgtt gtttgctttg ttcatgtaca tcgcctctcc
    10921 ttggctagct catgcttctg gtggcggaga agaactgatt ccaacgatgc gttctttgag
    10981 tatcgctgta ttggtttttc cttgtatgag tgtgatacga gggtattttc aaggcaatca
    11041 agaaatgatg ccttatgcgc tgtcgcagat tgtagaacag gtggcacgta ttttttatat
    11101 gctattggct acattcatca tcatgaaagt catggatggt gactatgtga cggcggtcac
    11161 gcaatcaact ttcgcggcat ttatcggtat gctagcaagt attcttgtcc ttttgtattt
    11221 tttgaagaaa catcaagcgt atacatcggc gtttattcac tacagcgaaa acaaaataaa
    11281 tattgcaacg aaggaattgt tgttagatac cgttaaagaa gcgattccgt ttattattgt
    11341 gggttcaggg gtaacgatct tcaaattagt cgatcagttc acttttatga agatcatgtc
    11401 tgcatccacc gagtattcca atgcacagtt actggatctt ttttcgattt ttagtgccaa
    11461 tccagataag ttgacgatgg tagttattgc attggcaaca tcgatcgcgt ctactgggtt
    11521 accgttgatt acagaagctg ttacgttgaa agatcgagta ggtctggcaa aattgacaag
    11581 cggaaattta cagttgtttt cattttttat gtttccggcg acatttggtg ttatgttact
    11641 tgcctatccg ctaaatacat tattttatac accggattct ctaggaagta atgtactgat
    11701 ccaagcaagt tttgtaggac tcttcctagg actatatatg ctagtttcca acatgctgca
    11761 aggaatgttt gaaaacaagg cggcaattaa ttatttggtt attgggtttc tagtgaaact
    11821 ccttcttcaa tacccagcca ttcgtttatt tgaagtatac ggccctttat tggcgacaat
    11881 gatcggattt gctgtgtctt gtaccttgat cttaaagaga atccatgaag tagcccgctt
    11941 taaaccaagt tttgtctggc gcaggacatt gctgatcttt attcttacct tgattatgct
    12001 tttggcttcg tggatcacaa agatgatttt tggtgctttt ttaaacgaag atagcaaatt
    12061 ccagtctcta ttgttgattc ttgtagtagc aggtataggg gctttagtgt atggttattt
    12121 agctttgaaa atccgcttgg cagaaagact actaggacca ggaatagcgc gtttacgaca
    12181 taaattaaaa ataaaataag tgaatcagaa gagctagatt gatgctcttt tgaaatgggg
    12241 gaaacgcatg cgtttagata agtttttagc agatatggga cttggcagcc gcaaagaagt
    12301 caaaggattg atcaaaaaag gaacgatcga agtcaatgga caagtggtga aaagcgataa
    12361 agcgcaagta aatgaattcg aagatgcggt tacttattta ggagaaatca ttacgtatca
    12421 aaaagatttt tactacattt tgcataaacc tgcaggagtc atttctgcta ctcaggataa
    12481 tcatgatcaa accgtaatcg atttattgga agaccaggat tatcgggaag atctttttcc
    12541 agtcggccga ttagataaag acacagaggg cctcttgatt ttaacgaatg acggggtgtt
    12601 tgcccatcag ttactatcac ctaaaaaaca tgtggagaaa gaatatctag ctgaggttag
    12661 cggtataatg acagaagaag atatccgttt atttgcagaa ggactgatta ttgatggcga
    12721 tgagcagaca ttgccggcaa aactccaaat tgatcaagta gatcaagaga cagaaacttc
    12781 aattatccgt ctgattttgc atgaaggaaa gtttcaccaa gttaagcgaa tggtcaaagc
    12841 agtaggaaaa gaagtcttat atttaaaaag aatccgtatg ggaaaatttt ggctgcctaa
    12901 agatttgaat aaagggcaat atcgacccat gaccaaagaa gagctagaac aagtgagaga
    12961 aaaataattg aatggatagc taaaagaata agcagagtag ttgtagcgcc atctgaaaag
    13021 cagacttgtc cgtctgactt tcggatggct tttttgtgaa atttcggctt atttccgtag
    13081 aagctacttc tggaacaccg tttgttcgga ataagggact gtgacaagac ttttgttaca
    13141 gtccccattg ttccttgtga ctcagagtta gatagtaagg acacgatctc cctaatactc
    13201 aggccaaaac gctgaattca caacctctga taaacggtgt tcaatcagct gaccctcggt
    13261 attagctcaa aaaacggcaa aatatgagga actattttcc cgtttttttc tctaatactc
    13321 aggccaaaac gctgatttca caacctctcc tatactttgt ttcattttcc ctatactttg
    13381 tttatttatt ctcattattg aaagcgctat aattacgttg cgaatgaatc gaaaggggtt
    13441 tttagatgaa aaaacgaaag gggttcaatc atttttttat gaatctatgg cgttataaag
    13501 cacttgtcct gatggctgta ccgggtatgc tttggatgat ttttttcttt tacatacctg
    13561 taatggcgaa tgtcgtagca ttcaaaaatt ttcatatttc agcagatggg ttcttggcta
    13621 gcttgaggga aagtccttgg gtgggttggg aaaatttcaa atttttattt tcttcggatc
    13681 aagcatttct catcactaaa aatacaattt tatataatgt agcatttatc cttttgaatc
    13741 tgctgatctc agtcgtattt gcaatcatta tgagcgagtt gagaaataaa cgactcgtta
    13801 aagtttatca aacgatgtcc ttactacctt attttctatc ttgggtcatt atcagttact
    13861 ttgtttacgc ttttttaagt cctgacaaag gaatattcaa tcagtggatc actggacatg
    13921 gaggagaagc tgtcaattgg tataatgaac cgaaatattg gccgtttatc ttggtgttca
    13981 ttggtacatg gaaagggatt ggttataaca gcattattta ctttgcatct gttatgggaa
    14041 tcgatccgac ttattatgaa gctgctatgg ttgatggagc tagcaaatgg cagcagatca
    14101 aacatgtgac gataccacaa cttattccac taatgacgat tttaacgatt ttagccgtag
    14161 gaaacatttt ccgagctgat tttggcctgt tttataacat tcctaggaat tccggtgcat
    14221 tatatgatgt gactcaagtt ttggatacgt atatctacaa tggcctgaca tccacaggag
    14281 attttggtat gacggctgct gcaggacttt accaatcagt tgtcggcttt gtcttgttga
    14341 tgatcacaaa tacgatcgcc cgacgttttg atagcgattc tgcattattc taaggaggaa
    14401 aaaaatgaaa aaaaagaaag tggagagagt ccaagtccgc tctttcaata agacggctaa
    14461 ttttttctct aatataatga ttgcactctt tgctatttcc tgtattttgc catttatatt
    14521 tgttatcgcc atctctttta ccgatgagtc catattgtcg actcacgggt acagtttttg
    14581 gccagaaaag ttcagtatat ttggttatac gtacttgttc gaccagatgc aggataaaat
    14641 tttccaagcg cttttcgtca ctgtacttgt tactgtttta ggtacactga tcaatagcac
    14701 ggctacttct ttatatgctt atgcgatttc acggtcaaac tttccattcc gtcgtttttt
    14761 taccgttttt tgtttgatta cgatgttgtt ctcaccaggg atggtggcca attatcttgt
    14821 tatgacaaat cttcttcagt taaaagatac gatttgggca ttgatcttgc caatggctgt
    14881 cagtcccttc aatattattg tgatgcgcac attctttaaa cgttcggtct ctgattcgat
    14941 tattgaatcg gcacggatcg atggagctag cgaactgcga atatttgttc agatcgtttt
    15001 accgttagct gttccaggta ttgcgacaat cagtttattt gccgcattgg gttactggaa
    15061 tgactggttc aatgctttac tatacatcca agatgataag ctagttccac tacagtatct
    15121 tttgatgaaa atccaaagta atattcaata tctgacacaa aattcaggag caggaagcca
    15181 attgacaggc ggattagcct cagttccagg agaatctgcc agaatggcga tcgtagtcat
    15241 ctcgacgttg cctattgcga tcagctatcc atttttccag aagcattttg tcaaaggtct
    15301 aacaatcggc ggagtaaaag aataaagaaa caaaaatata aatcataaaa ttaggaggaa
    15361 gaaaaatgaa gttatggaag aaactagcct ttacaggtgt ttcagcgttg ttgttgggaa
    15421 cattagcagc atgcggaggt tctggttcta aagaccaggc agaagctagc aattcggaca
    15481 ctttgcagat gtatcaaatc ggagataaac cagataattt cgatcaattg atggaagtag
    15541 caaacaaacg aatcgaaaaa gagatcggtg taaaagtcaa tatcaattac attggctggg
    15601 gcgattacga aaagaaaatg aacgtcatca tttcttccgg ggaaaattat gatatcgcct
    15661 ttgcgaacaa ctatgtttcc aatgcccaaa aaggagcctt tgctgatttg actgaattag
    15721 cgcctaagta cgctgaaaaa gcttataatg atttagatga agcatatatt aaaggaaatc
    15781 tggtcaatgg aaaattatat gctttcccag tcaatggaaa tgtctttgct caacaagtac
    15841 ttacttttaa caaagcttta ctagataaat acgatctttc aattgatgga atccaatctt
    15901 acgctgatgc agaaaaagtc ttgaaggaat tccatgaaaa agaaccaaac acggcagctt
    15961 ttgccattgg acaaggcttc aaagtccaag ggaactttga ttatccttta gggaatacgt
    16021 taccatttgc tattgatctg gatggagatg attcgaaaat catcaaccaa tacgataatg
    16081 aaacgtatat cggactgtta cgtacaatgc atagctggta caaacaagga ctcattccaa
    16141 gtgacgctgc aacgagcaat aaggattatc cactagaagg aaatacttgg ctaatgcgtg
    16201 gagaaacaca aggaccgtat gattatggcg atacaatttt gacaaatgca gcaaaacaag
    16261 aacttgtctc aaaagcaatt actgttccac taaaatctac agctcaagca caaatggcaa
    16321 attttgtcgt atcaaatact tcaaaaaata aagaaaaatc agtagaactt ctagggctgc
    16381 ttaacagtga tccagaatta ttgaatgggc ttgtttgggg gatcgaggga gaagcatggg
    16441 aaaaagtcga tggttcagat gataaaatca aattgctaga tggttatcag ccaaatacac
    16501 atatgtctgc ttggaataca ggaaacaaca agattctata tacacaagag tctattacgg
    16561 atgacatgat tgccaaacgt gatcaatcga ttgctgatgc agaaacttca ccgattttag
    16621 gattcagctt caacactgac agtgtcaaaa cagaattaag taatatttct aatgtcatga
    16681 atcaatattt agatggattg aatacaggga ctgttgatcc agatgaaaca ttaccgaaat
    16741 tgaaagacgc tttggacaag gctggctatg ataaggtatt gaaagaaatg caaaaacaat
    16801 atgatgagtt ccgacaagaa taataatgaa taaagacaaa ggggttgtga caaaagacgt
    16861 gtcacaacct cttgttccga ataaacggtg ttcaaaagcc agtttctcgg tattaagcta
    16921 aatatatgta aaaatgtgga aagccatatt cacaaaattt ttcttactac tcgaaactaa
    16981 gcggctattt cacaaactct tggtaaacgg tgttcaatca gctaaccctc ggtattcgct
    17041 cacaaaacga caaaatatga ggaactattt ttccgtttgt ttctctaata atcaggtcaa
    17101 ggtgctgatt gcccaacctt tttttgtata atgagaaagt ataaacttaa tgtttggagg
    17161 agaagatatg ttagggaagg ttttggaaga gttatttatc cggatctggg tcattataaa
    17221 actaaccttg tatttctgga tatatacatt tgccggcggg atcatctttg gattaggtgc
    17281 ggcatggaaa acagtcaatg aactatttta tttgtacgga ttcgagtata aagaaatcac
    17341 aataaaaaga ggctggaata tatataaaag aaatttttta agaggcaatc tgctatttag
    17401 tttattttta tctggtactg ctttattgag ttacaattta tatttatctg ttcaaatcaa
    17461 agggctcatt tttttagcta ttgattttat tttacttttt gccttgctct gcctatttgc
    17521 tacttatcaa tacagtatga ttttagatag tgaatatacg atctctgttc ctaatctgct
    17581 aaagctaagt tttatttcgg ttttttccag cttttcgtct tttctaaaaa taattattgg
    17641 tagcggtata attttaggcg tgacttggca attcaaagga ttgatcctgt ttggtgtcat
    17701 tggtcttttg acggtttgga atggtacgat gacaacacat tggcgtgaag aattagataa
    17761 acagttggaa agttatgaat aagtttcgga ttctattttc aaaaggaacc ttactaaatc
    17821 gtctgttgcg gaaatacgcg gttatcatga tcggtgtaac gatgactgct accgttattt
    17881 ttagtatcca tacttgggaa caaaatcaaa aacaggcaga aaacatgaca agtgatgccg
    17941 ttcaaagcac aagcagaatg ctgaatgata aaacaacctt gagtaggatc atcaaaaacc
    18001 agcttgtagg gaattccgaa aagatcgaaa atgtgacgac ctacttaaca aaacccatag
    18061 atcagtatct gatgtatgta tacgaacagc aaaacagtac cgatgaatta gtttctttcc
    18121 caaatcaaat caaagatctc tacgcaaatt atgaagaact aagtgcgata tatatcgtgt
    18181 tgaatcaact accggagtat tatgaatctt ctagagaaaa caaaggtggg gaaataaaaa
    18241 aggggacacc aaaattacta gacgcctttt atatcaagtt gcctatcaca caaggaggag
    18301 ctgagtcagg aagtattttt attggttttc aaaagaaaga actagatatg attttagaaa
    18361 atctaacgtc attcaatgga ctctctttat acatgatttc tggaacgaca aatcgactat
    18421 acacattcca tgataataaa atagctaaag aaacgtttga aagacaagaa atgatcatca
    18481 cagagagtct gaagaaaaat tcacaattgc cgattgacga tctggaaaaa aacaattttg
    18541 ttcaacacca agaactgtct gatgattatc gtatattagc tgtactagat aaaaaaagtg
    18601 taaaaatcga aacgggaaaa aatcttgccc cgctgatcat tggtatcatt attttagaca
    18661 atatactgct tatctttctt tataaaacat tcaaacgata ttcacagcaa gtatctatcg
    18721 tcatgcaggc catggatcaa acagcagcag ggaatttaga gacgcggatc gacataacaa
    18781 acacggaata tgaactgaag gaactttcaa tcggtatcaa cgaaatgttg gatagcatca
    18841 atcagtttgt agaagacatc tacaaattag aaatcaaaca gcaagacgca catatgcggg
    18901 cgcttcaagc acagatcaat ccacactttt tatataatac attagaatat atccgaatgt
    18961 atgcaatcag cgaaggaagt gaagaattag cagatgttgt gtatgcgttt tccgctttgc
    19021 ttcgaaacaa tacgaatcaa gagaaaacga tcactttaaa agaagaactg gacttttgcg
    19081 aaaaatatgt ctatttgtat caaatgcgtt atcctaatcg agtagcctat cattttatga
    19141 tcgatcctga tttggaaaag atcgaagtac ctaaatttgt gatccagcca ttagttgaaa
    19201 attattttaa acacggtatt gattttactc gattcgataa tgcgctaagt gtcaaagtct
    19261 tgcaggaagg caaacgggta aggatcatca tcaaagataa tggaaaaggg atgactgaaa
    19321 aacggctaaa acagattgaa gaaaaactgt ctcatcctaa agtggaactt cacggttcta
    19381 tcggtctgca aaacgtgaat gaacgtttac gtgccagctt cggcagcagt tattatatgt
    19441 cgcttgagaa taatgagaca ggtggactga ccgtttctat tacattcaag gagggatgag
    19501 cgtgtatcaa gtgttattgg tagatgatga atacatgatt ttgaatgggt taaaaaagat
    19561 aattgattgg caaagtttag gctttcaaat agtagcaaca gcagagaatg ccaaagaagg
    19621 actagctgta cttgaacagc gacagattga tttagtagta acggatgtga caatgccaga
    19681 aataaatgga ctggaattta ttgaagcggc tcaaaaagaa cgtcataatt ttgaatttat
    19741 gattttatca ggataccaag aattcgatta tttgaaagga ggtatgcaat taggcgccgt
    19801 gaattatttg atgaaacccg tgaataagtt cgaattggtt gaaagcctca agaaaatcaa
    19861 aacacggtta gatcagcaaa atgaacaaaa aaatcaacaa gaaatctatc aagaactgtt
    19921 attcagtcaa tggttgaatg aagaattgga tgaaataagt gaagaagaaa taataggaca
    19981 aataagtgag aagcagcata ggatagtatt gattcaactg gcaagaactc atgatactct
    20041 tatcaataga tggttgcaag aacggcaaca aatcttttac tatcaacgaa attacggaga
    20101 ccgtgtattg atcacgttgt tgttggaggc agatgaagtt gatggcttct gttgttttgt
    20161 tcaagaaaac tttcctgatc aggaatggct gatcagtatc ggggaagaga cagattcttt
    20221 ggacaaaatc cctgaaacat tccaacaagc gaaagacaat ctacagttgc atcaatttta
    20281 tggagataaa gacgagcatg ttttttacgc agtacaagca gaaacaaaag atcaaaccat
    20341 cgatttttca agtttcagac gcgtgctgca aaataaacaa ttggaagtcg ctcaaaaaat
    20401 gatcactgat ttttttgaac agtttcagtt agcagccatg atgccggaag atatacgata
    20461 ttccagtttt ttactgttta tggagattca acgggaattg attgatctgg aagatgagga
    20521 atatttacaa ggaatcgaaa aaatccatca ggcaaaaacg gtgcaagaac ttcagcagct
    20581 acttctgtca ttcatcaaaa agcatcagag acagaaaaag tacagtgaca atgtggaaaa
    20641 agtaatcgta atcctgcacc agcattatca agaaccactt acactcaaag aagtgtcaga
    20701 aaacctgcat ttaaatgtca tgtatctagg acagctgttc aaaaaagaaa cgaaaaaaag
    20761 tttttcagct tatttgaacc atttacgtat ggaaaaagcg aaacaactgc ttttgcacag
    20821 taatcaaaac atcaatgaga tagcaagtga gattggctac aacaacacca cgtatttctc
    20881 taaattattc aagaaaatcg tcggcagatc ccctaaagag taccgagaaa acagtggaga
    20941 atcataaaaa aaaaagccaa tgtaatgaaa taacttataa aaaattgtct agaaatctaa
    21001 ttttgggctg ttcttattac ttgaggcaag gcagcttttt catgacctct cgataaacgg
    21061 agttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgtg gaaagccatt
    21121 ttcacaaaat ttttcttaat actcgaaact aaacagcttt ttcacgacct ctccactaaa
    21181 tggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa tggcaaaata tgaggaacta
    21241 ttttgccatt tgtctctcta atactcaggt cagaacgctg atttcacaac ctctcaataa
    21301 acggtgttca aaagcttgca tctgcggtaa taagaccaac caaacgaaaa atatgaggat
    21361 ctatttttgc ttggctgttc ttattacttg atgcaggaca gctttttcac aacctcttgt
    21421 taattatagt ttgataagga aaaaggcaaa tcttttgctt gatatttctt agggtgaggg
    21481 atgatcccta attggtaaga gatcgtgccg cctttcagca aatcttgatg ggtaaaatat
    21541 aatttcgtat attctctgtc catccgtttg attttatgga taaattgttg ctgcggccga
    21601 ttaggcgttg cttcaatcgt caattccttt ccatttgaca aaagcaaaac agccttgtca
    21661 attaatggca taccgataac atattcaccg gttccaggcg tgacaggata aaatcccaga
    21721 ctattgaaga tataccaagc ggccatactt ccattgtcct catctccagg gtaacccatt
    21781 ggagaatcgt cgaaggtctg agtcatcaac tgtttaagca gtggctgtgc catttcaggt
    21841 tgtcctatat aactgaacaa gtaaggatag tgaaaacttg gctggtttga aatagctaat
    21901 tgtccaaatt ctatcgcggc catctcactc atttcgtgga tctcaaaacc atatcctcct
    21961 gggtcaaacg ttgggggctg attgcacagc tcaatgagtt tttctttata tctctcactg
    22021 ccaccatatt ttccgatcag tcctgaaaaa tcgtgataaa cagcaaagct gctttgccaa
    22081 gcacttcctt ctgcataatc ttgtccccaa cgattagctg aaaatggttc tctgaacttg
    22141 ccatctttat cttttgaacg cataaatcct gactcatagt caaagatatt ttgatagttc
    22201 aatgattgct gctgatattt cttcgtcatt tcctcttctt ctagtacctt ggcaacttgg
    22261 ctaatacaaa aatcgctata agcatagtct aatgtgtgat tgactgattc gtggtagtga
    22321 ttaggaacat agccatatgt caagtaatct tctgttcctt ggcgtccata atttggatgt
    22381 ttgctttgaa ccgtagcacc tttttccatt gccgttagaa attcaggcat caagtctcgt
    22441 cggatatctt ttacagcagc atctgcaatg accgcatcga tcaatgtccc aggcataagt
    22501 ccacgttcat caggagacag ccattttggc aaatagccac tttctctata gctgttcaaa
    22561 aaaccttcca gcatttcttc gtatttttct acagcaatca aagaataaag aggataaacc
    22621 gttttatacg tatcccaaaa cccattattt gtatagagaa caccaggttt tatagatcga
    22681 ctagtcgtat catagtggat tttttgccca ctaggatcaa tttcataaaa tgtttgtgga
    22741 aataaaaaca cccgatataa attatgataa aacgtccgca attgttgttg atcatggtgt
    22801 gtgagctgta tacgatcaaa ataggactgc cattgttctt gactgtgagc aaggtaagtc
    22861 tctttgcttt ggttttgttc tagttccagg ttataagctg cttgttccga gctgataaaa
    22921 gatgtcccaa atcggatagt ttgttttttt gtctgttcaa aatacaacgg gatcgagtcg
    22981 ctatttttgt tggaaacagc taactcatca attgttaaag gatattcaaa tctcaatata
    23041 aaataaaaag aaaaatcagg atcttctgaa ccagcaaaat gtgtgatttt tccttccact
    23101 gtgtaaggat ctataacaaa gagctgatgg cgtcccggaa aggataaaag taaaccgctg
    23161 tcatttttgc tgtaatcaat agataagatt ccgccataca tgctaggtat caatgtagaa
    23221 cagatgccat aacgtaattg acagatcgac agatgagtcg ggcaaaatat actttcttct
    23281 gggcggtaag aactttgagc atggaaaagg gtgttctcct gtagttttcc attgatcggc
    23341 gtaaacagca tatgactgaa atcacccatc caaggacttg gttgatgggt aagacggtag
    23401 ccttgaaaaa cgcggtcatc aggatgaaac caccagctgc cgttttgatc cgtagtttgc
    23461 ggtgcaaaat aattcatccc aaaaggaacg ccagtataag gctggcagtt cccattagag
    23521 aaatttggct gattcgctgt tgcatgtcta gtatctattt gaccgatatt cattttattt
    23581 ctcctttgtt gattgtcttt tgatgaatga aactggaaat ttactatcgg atggtcgatc
    23641 ttttgtctct aaccaagtga ccaaagcatc tcccgcaagc ttacctaact gttcaaaatc
    23701 ttgtgcgatg gttgtcagtg gcgggtcaac aagagctgct gcttggatat catcaaatcc
    23761 gataattgaa aaatcttcag ggatatgata accatgctgt ctcaagagat tcatggtctg
    23821 gatagcgaca acatcgtttt cacaaacaaa agcagttact tcatacttag tgatataaga
    23881 aagcaattca tctaatgtcg cgcttttgtc atccagtgaa gtgtggaaag tcaaaccagc
    23941 tttgtccaat gcttcaaggt aaccaaggta acgctgtcga acggattgtg gatgagtctg
    24001 cgtaccaaac aaataagcga tttttgaatg tccctgttcg atcaaataag aggcagcaag
    24061 cgcaccgcct tggaaattat cggaaagaac ggtaggaaaa ggtaactcat agattttttt
    24121 atctaatatg acgacaggga attctttcaa tgaaagctca aaaagtaagt caagatgttg
    24181 ttctgtatca agagcataat aaatcaatcc gtcaaattca ttgattagct cttttgctgt
    24241 tttattttgt agaaagtcaa gcgtcgtcat catgacatca atctgtttat cttgcatcac
    24301 agggttcaat cccttgctaa aatcaccaac agacaaatca gcgacatatg gaagaagaaa
    24361 cagaatccgt cgtgtttttg taggagaagc aagaatctgt tccggagaag cggatttaac
    24421 gaaactgcct ttcccgcgaa cgcgataaat aagtcctaat tgctctaatt cagtcaatgc
    24481 acgtttggaa gtgattctgc ttactttata ggtacgtgaa agttcttttt cagttggtag
    24541 ttggctgtct acaggtaatt caccagttaa aatagccgtt tgcaaatcat taaatattgt
    24601 ctggtacaat ggttgagtca tataaacacc tcttaatgat atatcatatt caaataatac
    24661 cataatggat gaaaaagtaa ataaaaagat attttttgaa agcgtagaca taattggctg
    24721 tatgaagtat aatctttaaa gatatatcaa atagaaagaa ggttttatta gtggtttata
    24781 aagatatacc aaaatcggtg cagaaattta tggaagatat cacacaaaaa tgtggggata
    24841 tccatgcgga ctgggcaaag aattttacag cagcatttgc caatactctg ttgacaacag
    24901 tcaaaaagca aaatgacgga accacctttt tattgacggg agacattcca gcgatgtggt
    24961 taagagactc tactgcacaa gttcgtcctt atttagtgat tgcaaaagaa gatgaggaat
    25021 tagctgacat gatcagcggg ttagtcaaaa agcaattttt ttatatcaat atcgatccat
    25081 atgcgaatgc cttcaacgaa gaagctaacg gtgctggcca ccaaacagat catacaaaaa
    25141 tgaacgattg gatctgggaa agaaaatatg aaattgattc tttatgctac ccaatacagt
    25201 tggcctatct tctttacaaa aatacaggaa aaactgatca attcaatcaa tcttttattg
    25261 aaggtatcaa aaaaatcttg gacgtgttca aaacagagca agatcatgaa cactctcctt
    25321 atacatttgt acgagacacg acaagaaaag aagatacgct gatcaatgac gggcgaggtg
    25381 caaaaacagc ttttacagga atgacatggt ctggtttccg tcctagcgat gatgcttgcc
    25441 gttatggata tctggtacca tccaatatgt ttgcggtggt cgtgttaggt tatgtggaag
    25501 aaatttttca aacgatactc gatgataaag aaattgtaca gcaaacacag aaattgaaga
    25561 aagagatcca agaggggatt cagaatcacg gacgaacggt taatcaaaaa gggcaaacga
    25621 tttatgcata tgaagtagat ggaatgggaa acgccacagt catggatgac agtaatgtgc
    25681 ccaatctgat cgccgcacct tacttgggat attgttctga acaagaggaa gattatctgc
    25741 aaacaagaag aacattgttg agtaaagaaa atccatattt ctatgaagga aagtacgcaa
    25801 aaggaattgg atcctcccat acgccagaaa aatacgtttg gccgattgca ttagctatgg
    25861 aaggaatgac aacgtcagac aaatctgaaa aagagcggat attgaattta cttgtagcaa
    25921 ctgacgcagg aacacacctt atgcacgaag gattcgatgt gaacaatcct gaaaattata
    25981 cgagagaatg gttttcttgg gcaaatatga tgttttgcga attagtcatg gattactttg
    26041 atattagagt agaaaagtag gaggtatcgt aaatgaaaaa gaaagtatat atcatttctc
    26101 atagccactg ggatcgtgaa tggtacatgg cttacgaaca gcatcacatg cgactgattg
    26161 aattgataga cgatctacta gagctatttg aagttgatcc gtccttcaac agttttcatt
    26221 tggatgggca aacgattatt ttagatgact atcttcaagt aaggccagaa aaaagacagg
    26281 caatccaaca agcaatcaat gaaggaaaat tgagaattgg accattttat atcttgcaag
    26341 atgattttct tatcagctcg gaatcgaacg tgagaaatat gctgattggc atggaagaaa
    26401 gccgtaaatg gggaacccct gtcatgttag gctattttcc cgatacattt ggaaatatgg
    26461 gacagacgcc tcaattaatg aaacaagctg gtatttcagc agcagcgttt ggccgtggtg
    26521 taaaaccaat tggctttgat aatcaagttt tggaagcaga aaactattct tcccaatact
    26581 cagagatgtg gtggaaaggc cctgatcaaa cagcaatctt tggtctgcta tttgccaatt
    26641 ggtatagcaa cgggaatgag attccagtag aaaaagaagc agctcttgcc ttttggaagc
    26701 aaaaattagc ggatgcagaa caatatgcct ctacgaacca cttattgatg atgaatggtg
    26761 ttgatcatca accggtacaa aaagatatat caaaagcaat ccatctcgca aatgaactgt
    26821 ttccagatta cgagtttatt cattctaatt ttactgacta tctggaagca gttcaaaaag
    26881 atgtgccaga agacttaggc tcagtagaag gagaattgac aagtcaagaa acggatggct
    26941 ggtatacttt agccaataca gcatctgctc gggtttactt aaaacaatgg aataccaagg
    27001 tccagcgaca gctagaaaat attacagaac cacttgcgac gatggcttat gaagttagtg
    27061 gaaactatcc ccatgatcaa ttagattatg cttggaagac attaatgcaa aatcatcctc
    27121 acgacagtat ttgcggttgt tcagtcgatt ctgtccatcg ggaaatgata cctcgttttg
    27181 aaaaagcaga tgaagttgga aagtatcttg cacaagattc cttagaacag ctgaccgcag
    27241 caatcgatac gaccggattc ccaaaagaca gctttccatt tgtcattgta aatactgcag
    27301 gaatggataa aactggtgaa gcagaaataa ccatcgaatt agaaagaaaa agattcgctg
    27361 aaggaatacc tgaacaacta tatcaagagt tagaaaattt gcctaaacgt aagtatcatg
    27421 tagaaacaaa aagtggggcc actataccag ctattttatc tgaagaaact gtccaatttg
    27481 gttatgattt gccgaaagac cgtttccgtg tgccatatat ggcaagaatg atcaaagtga
    27541 ctttaccatt agaaaacatg ccagcatttt cttgggaaac attcgcgctt gtagaagggg
    27601 aagcgaaagc agaagaaaag gaaacaatga ttcatcagag tggacgaatc atcgaaaacg
    27661 gccctctaca tttgacgatt gaaaaaaatg gtaccattac aatggaagat cgcaaaaaca
    27721 aacgaaagtt gaataatctg catatttttg aagatatcgg cgatatagga aatgagtata
    27781 tctttaaaca acctttctgt gatcagccaa tcctatccag taacaaagaa aactctgaag
    27841 taaaagtatt agtagataca ccggaaatag caaaaataag tattctacag gaaatggaaa
    27901 tccctgtttc tgcagacgag cgtttagaaa aagaacagca gatggttgtg gaatttcgtt
    27961 atcgaaaagc agaacgatcc aaagaaaaac gaatccttca aataaaaaca atcatgacaa
    28021 tcagaaaaga tagtaaaaaa atcgactttg aaacgactct cgacaatcag atgaaagatc
    28081 atcgcttacg tgtcttgttt cctacaaagt tacatgtgga acatcacgaa gcagatagta
    28141 tttttgaagt ggtgaagcgc ccaaaccatg tgtctaaaag ttgggaaaac cccacgaatc
    28201 cgcaacatca gcaagcattt gtcaatattc atgatgaaga gtacggtgta acagtcggaa
    28261 actttggttt gaatgaatat gaagtaacag aagacggaca aatcgcagtg acgctacttc
    28321 gcagtgtggg agaactaggc gattggggat attttccaac accagaagca cagtgtctag
    28381 gtgagcatcg gttcaattac agcattgaac tacatggacc agaagaaaaa ttctctactt
    28441 atttgcatgc atacgcagca cagattcctt tttccactca acaaatcaaa catcatgaag
    28501 gaacattgat ttcaaaacaa caatatttaa cgataaaaag tgaaacgttt gcaattacag
    28561 ctttgaaacg cagcaagttt agtgataaag tagtagtaag aggtttcaat atgtctagtc
    28621 atttagaaaa attggaaata actaaagaca atggaaaaac tgtcatattg aatctgcttg
    28681 aagaaccaac gaagcaagcg gttgtcccaa taatccaacc ttatgagatt cggacaatcg
    28741 gattcgagga ggaaaacgaa tgtatggcgg aatagaagca ggcggaacaa aatttgtgtg
    28801 tgcggtttca gatggtggag aaattttaga aaaattaagt atccctacca caacaccaga
    28861 agaaacattg gatcaggtat ttgaattttt tgatcgttat gaattgaagg gaatcgggat
    28921 tggctcattc ggcccgattg gtatcgacaa aacaaaagat aattacggat acgttttggc
    28981 aactcccaaa aaagggtggg cacaattcga ttttcttggc acaatcaagg aacgctacaa
    29041 tattcctgct gcttggacaa cggatgtcaa tgctgctgca tatggggaat tgaagcaagg
    29101 agcagcacaa ggaaaaaata gttgtattta ccttaccgtg ggaacaggaa tcggtgcagg
    29161 tgtcgtgatc aatgaagaaa ttttttcagg gatcgcacat cctgaaatgg gacatatttg
    29221 ggtgaaacgc catccagatg accattatga aggtacttgt ccttatcata aagattgttt
    29281 agaaggatta gcagcaggac cgtctattga ggcacgcaca gggatcaaag ggcaagatct
    29341 gccgcaagat catcctgttt gggctattca ggcttattat attgctcaag cactgatcaa
    29401 ctatacgttg acgttagcac ctgaaaagat tattttaggc ggtggggtaa tgaatcagga
    29461 tcatctcctt caaaaaatcc gtcagcaatt tgttgaattg atgggtggtt atatggaaac
    29521 acctcaagta agtgaatata tcgttcgttg gggactgccg aatgaaagtg ggattatcgg
    29581 tagtttgctg ttagcagaac acgaacatca aaagtaagca gacaaattac acaaaaagaa
    29641 cagagtctta tcattatctg ctgataagac tctgttcttt tttatttcta ttcccataat
    29701 ccaggtaaag ctttaccgac tattttttct tgagtaaatg gatggatcat gtgcagttct
    29761 aatgcatgga gcatcagtcg ttcagctgtt ctattttggt agagagggtc tccaacgatc
    29821 ggatgtccct tatgagctaa atgaactctg atctggtgtg tacgtccggt ttccaaaaca
    29881 caatagactt gtgtttgttc agaaagtact ttgttgacag tgacatgtgt caaggcgctc
    29941 tgaccatttt tatcatctac gacccttttt ctccgatcat ggcgatctcg tccaatttta
    30001 ttggtgatgg tcatatcttt ggcaatttct ccccatacga tggcttgata tctccgatag
    30061 atcatttttt gttccaacat tcttccaagt atcggcaaga caaatggatt ttttgcaaat
    30121 aaaacggcac cgcttgtttc tttgtccaag cggtggacaa cgtaaggtcg ttgctgttta
    30181 gtgtcgagat aagcagctaa atgattgagt aaggtactgt tttctgtcgg ttcatttgga
    30241 tgagtcttca ttcctgcagg cttatccact ataatcaaat gctcatcttc atataaaaca
    30301 cggatgtgct tcgcatctcc taattgaact tcttgataat tataatctgt ttcttcgaat
    30361 aacaatgtga tctggtcatt gccctttact tcatgatgga acaaagcagg ttgttgattg
    30421 atccaaacgt ttttacgtat acgcaaaaag tgtcgaacct ttctaggaac taaccattcc
    30481 ttttcaagca agtcgcgaat tgttgtttca ggatggttca taggtaaagt gatcgtgatt
    30541 ttcacaagtg tactcctttc agtgcaatcg cagactaatt gtaccatttt taaaacatat
    30601 ttaagaaaaa acttacattt ttctgttcat tcttatgtta ctctacttgc actaacgtaa
    30661 aaaaacatat tgaactacaa cttaagcagg aagaaaacac tctttcacta tggtaaaata
    30721 agacgattga atcaaataga caggagaaga agcaaatgga ttttcaaagc attttagaaa
    30781 agataaaagc tgctttgatc agtttttggc gttggatcag accatatctc ggacaattcc
    30841 atcgatggcg aaagcgcatc tggaaaaaat atcatgtgaa caaattgatt ttgctgattg
    30901 gtttgatctg tgtcttagtg acaagtatct acctctttat tttagcaaaa caagcaaatg
    30961 tcgaaacgtt aaaatcagga ttaagccaat cgacagtcat ttatgataag aaaaacgaag
    31021 aagctggcac actgtacgcc caaaaaggaa catatgtaga attaaacgcg atttctccct
    31081 atatccaaga tgcagtaatc tcgacagaag ataggaattt ttacgagcat cacgggtttg
    31141 atatcaaagg gattgcccgt gcagcagtcc gaatggtctt gtctggcgga acatcagggg
    31201 gcggaggaag tacgatcact cagcagcttg ccaaaaatgc gtatctgaca ttagaccaaa
    31261 cgtttgatcg aaaagcgaag gaattatttt tggcaatcga gatcgaaaag aagtacagca
    31321 aagaagaaat cttgaccatg tatcttaaca atgcctattt tggcaacggg gtatggggtg
    31381 tccaagacgc ttccagaaag tattttggag tagatgcaac gaatgttacg ctgtctgagg
    31441 ctgctacttt ggcaggaatg ctgaaaggtc ctggtattta taatccgatc gatcacccaa
    31501 aaaatgcgaa tgatcggaaa aacacagtac taagtgtaat ggaagaaaac ggaaaaatca
    31561 caaaagaaca agaacaagca gaaagccaga cagatatcag cgcgtatctt aatgacacat
    31621 acgataattc agattctggt tatcgttatc cttattattt tgatgcagtg atcgatgaag
    31681 cagttaacga gtatggtctt gatgaagaag atgtcatgaa taaaggctat aaaatctata
    31741 cagctttaga tcaggattat caaaagcaat tagaagctac ttatcaaaac agtgcattat
    31801 tcccagcaaa tgcttcagat ggtgccatgg tccaatctgc ttcagtagct ttagatccga
    31861 atacaggtgg tgtacaagca ctagtaggac gaagaggaga tcatgtcttt agaggattca
    31921 gctttgctac acagatgcgt cgttcaccag gatcaacgat caaaccatta agcgtatata
    31981 caccagcact ggaagctgga tacaagcctt ccagtatctt gaaagacgaa ccgcaaagtt
    32041 attacgatgc gcataatttt gacgggacat accaaggaga agtaccgatg tatcaagctg
    32101 ttgcacaaag tttgaatttg cctactgtat ggcttttgca tgagatcggt ttacaaaaag
    32161 gatatgataa agcagaagaa ttcggcctgc ctttagcaaa atctgataag tattatggct
    32221 tagcccttgg tggactggaa aaaggagtat ctccattgat catggcgggt gcttacagtg
    32281 catttgccaa tgatggtcag atgtatacgc cgcatcttat cacgaaaatc gttgattcga
    32341 ctggagcggt gatcgtcgat aacacaaaca aaaaaccaaa acaggtcata tcaaaggaaa
    32401 cagccgatga aatgaccagc atgcttcttg gtacattttc aaatggaaca gcggctgctg
    32461 cgaatcctgc aggttatacg atagcaggga aaacaggaac cacagaaact aatttcgatg
    32521 ccacaaaagc aaatgaccaa tggatgatcg gttacacgcc agacgtagtg atttcgacat
    32581 ggatgggtta cgaagaatcc agcaagctgc actatttgga agggacaagt ggaaatgttg
    32641 tcgggaaagt cttcaaatca gcggcagaag gcatccttcc gtatacttca aaaacaggat
    32701 ttagtgtagc agatgcctat gccacaggag gacaagtggt agctgcagat caagttggaa
    32761 acacaggaaa ttctaatgga gagtctggaa attggcaaga taacttgaat caatatggtg
    32821 aacaagcaaa agaagggctg aaaaacttcg gcgatatggt gaaagaaggc gttcagggaa
    32881 ttggtgaagc ggctaaagac ctctggcgga aatatcaagg agagtagccc ctttgaaaac
    32941 gaataacccc atgttacaat agagagtaac tactaaaaga aaagagggca taacatgtca
    33001 aacatctacg atagcgcgaa tcaaatcgaa cgtgagatcc gtgaattacc agaattcaaa
    33061 gcgttgaaag aagcgtacga gaaagtacaa gcaaacgaag aagcacacaa actgttcaaa
    33121 gacttccaag cgatgcaaat cgaattacaa gaaaaacaaa tgagtggaca agagttcagc
    33181 gacgaagatg caatgaaagc acaagaaatg gctgtgaaaa tccaatcaga agaagtaatc
    33241 aatgacttga tgcaaaaaga gcaaggcttc agtacaatca tcaatgactt gaaccgtatc
    33301 atcatgacac cagttcgcga cctttacagc gaataaaact attttatgaa acaacagagg
    33361 gtgtgaaaga gacgttcggc cttgagcgat gacaaaattg cttcttattg ccgataggct
    33421 gtctcttgaa caccatttac caagaggtcg tgagcctacc gtttaactct gagttacaag
    33481 gaacaatttc aaaaaacagc ttctcatgtt tgatgactat tgtgacttgt gaccgaggag
    33541 ttggcaggag aacaccgttt attcagaaat aggagctacg acaaaagtct tgtcatagct
    33601 cctatttttt gtcattcatt tttcaagcgg tagaaatagc ctttttgagc taacagtttt
    33661 tcgtaagtac ctatttcaac gatttttcct tcatcgatca caacgatttt atctgcctct
    33721 ttgatcgttg aaagccgatg agccacaata aaagtagtgg gtgtttctgt caggtaattc
    33781 agggcttgct ggattttttt ctctgactgg ttatctaaag ctgaggttgc ttcatctagc
    33841 aaaatgattt ttggctgacg tatcaacgct cgtgcaatgg agatacgttg cttttggcca
    33901 ccagacagct tgttgccatg ttcgcctact aaggtatcca gaccatcagg aagctggtca
    33961 atcagtgaag aaagttgcgc agcttcgatg acttcatcta aacgttcttt tgatacgttt
    34021 ttcaagccat aagtgatgtt ctctttgatc gaagcggaga acaacaaagt ggtttgagga
    34081 acaacggcta aataatttcg cacacttcgc atatccagtt catccaaagg ctgatcatct
    34141 agaaaaattt ttcctgaagt cggctggata aatccaatca ataagttaag gagtgtagat
    34201 tttccagagc cagatggccc aacaaaagca attgtttctc cagctttcac ctctagagaa
    34261 aaatgatcga taatcggttt gagtgagtcc ggataaagaa aagaaacatc ctcaaaacga
    34321 aaatttccat ccaaatgttg gatttttttc ttaccgtgat aagtttctac atcgccagca
    34381 atcaacagtt cgccaataga agtgatagat tccatgcctt ttgaaatcgt aggaatcagc
    34441 gtgatcattc cagaaacgga attgacgatc gtcgtgaaat acgtctgata catcacgaca
    34501 tcgccgggtt ggatgcgccc ttgtaaagct aatatcccag tgaaaatcaa acaaatcagc
    34561 tgaaatatct gaaaactagc ccagctgact gatccgaaaa cagattgcag gatatctaag
    34621 tggaatcctt tttcatagat ttgagtaaaa tgcttattta agcgagttgt ttcgtgtttt
    34681 tctaacccat gagctcgagc aatcggtgct aattcgatca cttcgatcac ttttgcagaa
    34741 gtctcttcca tttcttgcct gaagtctgag ttggactgtc tgatcctttt acggaaaaag
    34801 agagtaacga atgcggcgag aggaacagtc aataagaaaa acatgaaaac aacaggactt
    34861 ttataaagcg tgatacctaa agctacgaat aaattgacag cgatatttaa tccgctgaca
    34921 aatacttgag aggacaacgt ttggatagct tctacatcac ggatgatctt tgactggatt
    34981 cgtccagatt gcaattcttt ttgataagga atagatagtt cttggatttt cttgataagg
    35041 gtcgctctta gtccagcttc tacttgacgg atcgaagtgc tatggtattt tacatgaaaa
    35101 taattcgtca atatattttg accaatcaga caagccatca aaaaagcgtt gaaataaatg
    35161 gcctgcaact gagaagaatc tttcgatgaa gctgcattga ttacattggc aatagcgatc
    35221 ggcattaacc aagatggaag atgtttgata ataaaaaaga gtcctgacat ggcaaaacgc
    35281 caataatttc cctgataaag atgcagcaat gtctgaaaag catttgcatg atatttttga
    35341 taacttttct gtattcgttc ctccataatc ttctccttaa caatcttggt aaataaactg
    35401 caagtgttct attgcttcag gaacataaat ctcaggagta gcttcaagag tggcgtacat
    35461 tagaggacgc tcatatttga tataatgcag taccgggcgg aaatctaaac agcccttgcc
    35521 aacaggtacc atgcggatct ttccattttc catgatatag tctttgagat gaagcgctgt
    35581 aatataacca tgtaaattgt ccaaggcacc tcggatcact ttttcctgat cagcatgatt
    35641 ttctttatgc atcaaattcg cacaatctaa aatgattttt aaatttggtg aagctacttc
    35701 atcgattaaa cgtttagcta actgatagtt atagattgga tgattgatcc ctgcttcaac
    35761 agcaacagtt acgccaaaat tttctgcatg agcgactaat tcaatgatag aatttctggc
    35821 gatctgataa gcttcttcag taaaattttc ttttgtgtaa ccgacagaga cactaccggt
    35881 ttccgtagca atcatcttgg cttgataggc agagcacaga gcaatttgct gtttgaaagt
    35941 tgccaatgct tgtgcacgaa cttcttgatt tttgctggat aggttgatat agcatcctaa
    36001 aatggaaagt tcgatattgt ttttttggaa gtattcacca aaataggcag cactcccaag
    36061 tgtcatgtca gttaatttaa gttgttcagg aaatgctttt tgtggtgcaa attggatatg
    36121 ggacaagtga agctgtgata ctttttgaac gatttcttca cgatttcgaa catctaaatc
    36181 gtgggcacgg atgcctaaat ttaatggaaa catctcaaac tcctttttgt aaataattag
    36241 caagaggcag acgggattct tttatcaatt caacaattat tcgggcaact ttatcagcgc
    36301 cttctggact aaagtgggta ttgtctttta gtccttctgg gtaattttta caagtatcag
    36361 gtataagatg caggtagaat ttttttgtct cttcttttga gaaggtctgg aacaattctt
    36421 gtgatcttga gaacacatct aaaagaggaa tatggtgttt ctcagcaaat gcacgcatcg
    36481 cttttggata gtctccaagt atatctggat tcaatgttcc gttttctaag taatctcgtc
    36541 gcgtcacaga tgtcagtaag accatttgga cgttcgcttt ttttgcgcat tcaaaaaata
    36601 tctgcagatt ttttaaatag ctaccgaagg gaagcgtacc tctttcttct tggattttct
    36661 ggtcgttatg tccaaattgt acaaataaga aatcattcgg ctgaaactgt gaagctagac
    36721 gggctaacct tccttcctgg ataaatgatt tggtgctgcg tccattcttt gcttggttat
    36781 tgatctggat ctcgggtgaa aaataagcag agattttttc tccccaacct gtctcgggac
    36841 gcttgttttc atctttttct gccgcagtag aatcaccggc aatatggata gtaggcatgt
    36901 tatctcttct ttcataaagg tttataggca atgaagcaga acagcaagag aagaagacgg
    36961 agcaaatttg gtccgtcttc ttctctaaaa cgtggataaa cggattccgt tatttcacta
    37021 tcctgttttt gattcgttac tgttactatt aatcaattag gcaaaaaagc caaagtaatt
    37081 tttagcattt gtataactaa tcgcttgaac tagacttcct aaaagttgtt catcttcagg
    37141 tgcttctcct cgcatgacca tttcgcctaa aaattcacaa agaacacgac ggaaatattc
    37201 atgtctagtg taggacagga aacttcttga atctgtcagc atgccgacaa aattccccaa
    37261 agcactacca tcagcaaatt gtgtcaattg atcgcgcata ccagctcgag tatcgttgta
    37321 ccaccatcca gaacctaact gcagttttcc attctgttct ttttggaaac agcccatcaa
    37381 agctaaaatc gcaggatagt cattcggatt caatgaataa aggatcgtct ttggcaactg
    37441 atccgtttct tctgcacggt tcaataattt ttgcagagga atagtcaaag aaagatcatt
    37501 cattccatca tagccggtat cgggccctaa tttttccaac atttgtgtat tacagttacg
    37561 ataagcatgg atgtgcagct gcatcgtcca attatgagaa tgatagaagt gggtcaaatg
    37621 tatcagtgtt tctgtacgat aagcatcgat ttcatctgaa gataatagtt tgttagctcg
    37681 tgctttttga aaaatctctt ctaatactgt ttcatccgct tctttatagc gtaagatgtc
    37741 taaagcgtgg tcggaaacac gacatccatg ttgatggaag tattctatcc gctgccctaa
    37801 gacttctatc agttcttgat aggtggtgat cgtttggtca gctgcttctt ccagtagtgt
    37861 gacccatgca ttaaatcctt ctttttcaat attgagtgct ttatctggac ggaatgaagg
    37921 tagaaccttc aaatctggtt cttcatgagc aagcaactga tgatagtgca agtcgtctgc
    37981 gggatcatct gttgtacaaa tcacttctac attcgatttt tttgcgattt cccgacggcg
    38041 aaaatcagga gctgccaaaa tttcatttgc ttgtgtccaa attttctctg cattttcttc
    38101 atttatcaac aagtcaatac cgaagaaacg cttcaattca agatgtgacc aactgtaaag
    38161 cggatttccg ataatttttg gtacggtttg acaccaagct aaaaattttt caaaatcaga
    38221 agcatttcct gtgatttttt cttcaggcac gccgcaagct cgcatcaagc gccatttgta
    38281 atgatcgcct cctaaccaag cttctgtcag attgctgaaa ttgttgtttt cgtaaatttc
    38341 ttttggatct aagtgacagt gataatcaat aatgggcatg ttttttgcgt aggaatggta
    38401 aagctttttc gcccaatcgt cttttaataa aaaatcatca gataaaaaca taaatcttcc
    38461 ttctttcttt ttatactgtt ttatgaaaat caaagtgtta ctccgttttt aaaaatggca
    38521 atttctcgaa cgtcattttg ttcatttttc gtttcttctc cactagctac agcgatgatt
    38581 ttttgaacaa acgattctaa gattacttcc attggttgtt ctaataagac gcctgcatta
    38641 aaatccatcc aatgaccttt gcgttcaaaa agagttgtat tcgtagatac cttgaccgtt
    38701 gggacatatg agccaaacgg agtccctctt cctgtggtaa acaagaccaa ctggcaatcg
    38761 gatgaagcaa gagcagaggc agctacaaga tcattcccgg gagcttgcaa taaactaagc
    38821 cctggttttc tgattttttc gccatattgt agcacatcaa cgacaggaga gcttcctgat
    38881 ttttgcgtgc agcctaaaga cttatcttct aatgtagtga tcccacctgc tttattacca
    38941 ggagatgggt tttcgtaaac tggttccccg taagacaaga aatactgttt gaagtcattg
    39001 atcaagtcaa cgattgagtc aaaaacttct tcattttccg ctcgtgccat caatacttgt
    39061 tctgcgccaa acatttctgg cacttctgtt aagatcgtgc tgcctccttg agcaatcaag
    39121 tagtcagaaa atgcaccaag taaaggatta gcagttattc ccgatagacc atcagaaccg
    39181 ccgcatttca aaccgacatt caacttagag agcgggactg atatgcgctg gtcattttcc
    39241 gccgctgtta aaagctcttc taatagagcc acaccttgtt cgatctcatc gtaaacatct
    39301 tgagccacta gaaatttcac tcgttcttca tcatattcac ctaaaatttt tttgaattca
    39361 ggaactgtat tgttctcaca acctaatcca aatactaaaa ctcctccggc attgggatga
    39421 tttactgcgt ccgctaaaat ttctcgagta ttttgatgat cttttccaag ctgcgaacag
    39481 ccataaggat gctttaaaat cgtaatatgg tcaaactgtc ccagatctgg atgtttttcc
    39541 ttgaactgtt tgacgatcag ttcagcaatg ccattcacac acccaacggt aggtactaaa
    39601 aacaaatcat tacgaatccc tacttttcca ttttttcgca agtaaccttg aaatgttcgg
    39661 ttctcaaaaa ggtaatgact tggatggaca tctttttcat atcgataatc caattcacca
    39721 gaaagattgg tttttacatt atgagtgtgc agccattgac cggcagttac gtctgtcgtc
    39781 acatgaccga ttggatagcc atatttgatc acgttgtctc cagctttcaa atcacccaat
    39841 gcaatcttat gaccttgcgg gatatcttca agagtggtga tcgtttgctg atcaatcgtc
    39901 agttgtgttt gttttggaat aggggttaat gcgacgacca cactgtctcg tcgatttaat
    39961 ttcattgttg gttgcagggt ttcaagcatg gaccatcgtt cctttcattt cttgtaatac
    40021 tgaccggaca cctttttgat cgatctttgc agctatttct tgaactgtat ctgtcacatt
    40081 cggtatttga gacaaatctt ctccccaaag cgcagcgttt tgcaataatc gaggaatagc
    40141 tgttccatta tcagaccaag catcagtaaa ttcgcttatc gtcttttcat catcatgtgg
    40201 ctttacttga tcgcctcgat acataagaag tagcgcagct agtgaaacag tgattaacgg
    40261 tggtacctga ttttgttctt cgatatactt cttgaacact ggaagcagac gggtgctgaa
    40321 ctttgaaaca ctatttaatg aaatcgcttc taatttgtga tgggcaaaag gattcaagaa
    40381 acgttctttg atttggtcag cataaatggc taattcatct ttaggtaaag aaagcatagg
    40441 aatcaattct agattgaata actgatcaac atatgcggag aataaaggat ctttcattac
    40501 ttcttctaca gtttccaatt ccgccaacaa agcaagaggg accatcgctg tatgcggtcc
    40561 attaagcaaa tgcaccttac gttcacgata aggagtcata tcatctgtga tgatgacatt
    40621 caagccagcc tgtttgaatg gtagttgatt ctccaattct ttaggccctt cgatcaccca
    40681 aagcatgaat ggttctgctt tgaccatcaa ttgatcttca tagttatgcc gttgattgaa
    40741 aacacctgca tcttcgcgag gataacctgg gacgatgcga tcaactaaac tacaacagaa
    40801 aacattttct ttttctaacc actcaacaaa agcaggttct aaatcccatt tttctgcgta
    40861 gcgtaaaatg atttctttta gacgttcgcc gtttctgtca atcagttcgc aaggaatgat
    40921 ggtaaagcca gctttgttta atttgaaacg tcggtataaa aaggctgtca gtttgcccgg
    40981 gaagctctgt gggggagtat ctgttaaatt atcagtctcc acgaattgga ttcctgcctc
    41041 tgttgtattg gagacgatat aggccaagtc gtcattttct gctagagata gataagcatc
    41101 ccattcatca taaggattga tcatttgttc tactacggtg ataatctcgg aagtatcaac
    41161 gatttcgcca ttgctgagtc cttgtaaaat gaccgtatat aaattatctt gctggttcat
    41221 catatttcca agaccttgtt ctaaaggttg aatgattgca acattacctt taaacaattg
    41281 ttggttattc agctgctgaa tctgccaatc aataaatcca cgcataaaat tgccttcacc
    41341 aaactgaaga acttttacag gtgcttcgtt ttttttcact aattcttttg tcagtttttt
    41401 catataaact tcctttctgt gttatgcaca cgttaacatt tttatataaa attacttgta
    41461 gtttacaagt aattgatcga ctcacgaagg atcaacaagg tggagagctc aattttagat
    41521 tgttcgatcg tcctgccttc gatcaaatct agtaaaagct ttgttccttc ttgacacatt
    41581 tctttcaaag gcttacgtac ggtggtaagc ggcggattga gataggagac gactggagaa
    41641 tcatcgaacc cgatcagaga aatgttctgg ggaacacgga ggccagctaa atgtacagct
    41701 ctcaatgcac caatcgctat gtcgtcgttg ccgcaaaaaa ccagagtagg caggtcagaa
    41761 agtgataata ggtattccat gttttggagg ccgctttcta agctgtaatc tcctgttaaa
    41821 aaataggctg gattgacagg gatgctcgct ttttttagac ttttcaatac ccctttttcc
    41881 cgttcttttg tcgaacgaaa atttttcttt ccgccaatat aagcgatctt cgtatgtccg
    41941 catccaatac cgtaatctac tgcctgcata acaccggccg catcatcggc agtgacgttg
    42001 ataaccgctg gatcattgat gtgacgattg acaacaacga aaggaaattc tttttctttg
    42061 agatagtcga tgaaaggctg atcatcatca ctttgactca taacgatcag tccatcgtag
    42121 cgttgcaggt tgatttctgt taaatcaaca acatcgatac tttcaacaga taaactgtaa
    42181 tttgtgtcta atactgaatg gatgcctgca atggaatctg ctaggaaatt tgatgaagtt
    42241 cctttatgca aacttgagaa aaatagtccg attagatatt ttttttgatt cactaaacct
    42301 tttgcactat agtttggcga ataattcatt tccttagcta gttgtcgtat tttttgtttt
    42361 gtttcatttt ttattaaact gctgttattc aaagcacgag aaactgtcgt atgagaaacg
    42421 ccagctgctt tagcgatatc tttgatcgtt accattccat cattttcctt actttttcac
    42481 tttttgttaa cgcattcatc atattacgat aactacgata agtcaataga tctttgatat
    42541 gaaaagattg cacacgttaa aaattacttg gatttttatt tttaattatg ctattcttga
    42601 aagcggggaa tgctcattta attagatgag tatcaatttt acagaaaaat gaaagccttt
    42661 tattttaaaa ggaggagagg aagaaaatga agaaaagtac agtattttat aaaatatttt
    42721 taccgatgtt tttattagga gccgtacttg tcataggatt tagtttattc atttaccaga
    42781 atacctatga atcgatcgaa tcgagttatt taacagacaa aaagaatttg ctcaggcaag
    42841 ttaaaactaa cgttgaatgg aaaattagaa cgattgaata ttcgttttct acgtatggtt
    42901 cgaccaaaaa tttctcggac atattcaagc atccgctgac atatacagat tactccactt
    42961 attctgaaat caggaaagaa ctaaatttta tagaaacgat tgttatggat gataataatt
    43021 atgatttagt cagtttggct ggtaaatggg gagtaatcaa tggatcgttg agtcaactga
    43081 ccgaagagga agttgaaaag tatcaacaga agtacataaa taacaaaaat aatttatttt
    43141 ggcaaaagaa aaacaaagga atcgaaatgg tgatttcttt gccaatgttt gaaaaagaaa
    43201 aatatgcatt gggaattgct acgatagaga aacatacaat agaacaagtt gttggtgaaa
    43261 gtggagaaga catactttct attgaaaacg aagttggcaa cctttttact actcagacag
    43321 taaaaaataa agagcaacca actgtattaa cggacgtgga tctagattac aatcagctga
    43381 aagtgataag agaaaaagat aaaacgtata tagtactgaa atcagattat aataattgga
    43441 tttacagatt agaagttgac tctagtgcaa ttggcgcaat gatccgcaat ctgagaatcg
    43501 ggctagttgc agtgtcttta acactggtag gactcattgg tcttctttcc tacttatttt
    43561 cggacagatt tgcccgtccg atttctcaaa tacaggaacg tctgaatctt aaatcaaaag
    43621 gattcagtgg gaaagaacta gatttggtcg ctcaatcagt cagtcggatc ataggagaaa
    43681 aagaagcgtt aagtgcacac ttagttaccc aaaagccaca gctggaaacg ctatttgtac
    43741 ttagtttatt tagaaatcga gtagaaaggc gagaactaga tcagcggttg cagcaatttg
    43801 gttatagttc tcaaaatgac tgctattata cagctttggt tcaaatcgat ctgttagaag
    43861 atagtcacgg tggggagcgt gatttgctac ttttagcgat caacaacatg atcacggaaa
    43921 ttgtgccaaa ggaaaatcga atgctgccga tcgttttgaa tgaagaaatg caagccacta
    43981 tttatcgaat ggataaagat gatgcagaag caagcaaaaa agtcatggag tattgccgac
    44041 tcattcaaaa acgtgtaaaa gaatacttga atttaactat cagtataggc atcagtaacc
    44101 ggttcgaaac gctgaatgaa agcaagcaat cggttgatcg ggcaaaagaa gccctgtatt
    44161 atcgtgtaaa tactggtcca agttcaatta ttttttatca ggaaatcgtt cctgctgctc
    44221 atgaaaaaac acttattaga tatccgatag aagaacaaaa tcgattgttt gaagccattc
    44281 gttctggaaa tcaggaagtg aagaatttag tacatgaatt gattgatgca ttgtttgcac
    44341 aaaataaaaa tcctttgagt cgggaagtag tttcggtccg cttgatcaat gaactcgttc
    44401 aattaggaca gttattggga gtagattcaa aaaactttga taacatgaaa caaatttatg
    44461 taaaagcact gaacaattat catcccaaag aactagaaaa acttgtttta gaacaattag
    44521 ttctgccaat cacagaatgc tctcaagcaa tcactgatca tgaatttaaa aatttgtcgg
    44581 agaatattat gcatatcgtc tacacagaat atgatcgtga tctgtcttta gatgtgatcg
    44641 ctgataggtt gcattataac cctaactatt taagtaatgt atttaaaaag gaaacaggag
    44701 aaaaatttgg tgattttgtt caaaatcatc gtttagaggt agcaaaaaaa tggttgaaag
    44761 agactaacct ctccgtaaaa gagattgcag aaagattgca atatcgaaat ccgcaaaatt
    44821 ttattcgttt ctttaaaaag aaggaatcga ttacgccggg agaatatcga aaaaaatatt
    44881 tgtgaatcaa gaggctgttt ttcttgtgga ctgattcaag caataaaaga agttagaata
    44941 accctagtat ttgtatttat ggatagttaa atttcaaggc taagcacgca aaaagaactg
    45001 tgacaaaagt cttgtcacag ttctttttta cgaataaatg gcgttcaaga gacaggctat
    45061 cgacaataag gcataatttt gtgaaatatg ggaagctatt tttcaaaatc gcttcttatt
    45121 gctcctgtgg gttactcgtc gcaatgtttg tgagagtttt tatcatcgct caaggctgaa
    45181 cgtctctttc acaacctctg atacacggtg ttccaggagt agattcttcg aaaaataagc
    45241 cgaaatttca caaaaattta agaagcaatt ttcgtaaatg ccttcttatt tcttgaagct
    45301 gaccacttct gacacaacta ccattttgcg ttcagtacat aatcaccctt tgacagaacc
    45361 tacgagtgct cctttgacaa aatatttctg aacaaaagga taaacaatca gcataggcac
    45421 tgttgcaata acgataacag ccattttaac tgtttgtgct ggtggaatca catcaacggc
    45481 actactttct gcttgcatcc cacttgatac aatgacgatt tggcgtaaaa gaatctgtat
    45541 cggccattta gatgaatcgt tgatatacaa aatagcagtc atataagtgt tccagtaagt
    45601 aacagcataa aaaagcgaga tagtagcaat agacggtaac gctaatggca gcataatttt
    45661 gaaaaataca ccaaaatctg tgcaaccatc catttttgct gattcttcca aactatccgg
    45721 caaggcttgg aaaaagtttc tcataatgat catattcatt gcattaacag ccacaggcag
    45781 gatcatggcc cagtaagaat tgaccagccc tagagaacga acaactaaga acgttggaat
    45841 catccccccg ctgaacaaca tagaaaatac aacaagaaag ttgacgaaac ctcgaccaaa
    45901 caaatacttt ctggaaagac cgtaggccat cagtgaagtt gcacacatac tgacgatcgt
    45961 tccgacacct gtaacaccaa tcgaaacagc aagtgctcta aaaatggtcg gcgtagaaag
    46021 aatgtagcga tacgcgtcta aagaaaaggt agtaggaaaa aggataaact ttttcgcgac
    46081 gacttcttgt gtagaagcaa aagatgtagc gatgatattt aaaaaaggaa cgatacagat
    46141 caaagctagc aaaattagaa acgtattgtt gaaaagtgta aaacttcttt ctcctataga
    46201 gactttcttt ttcatataga taggctcctc ctcaaaataa attaaatgtt tctgtttcta
    46261 tatgttcaat ttatcatcgg tgaaagatta cgtatataat catttcctat gctaagtgtc
    46321 agatagaaaa aaatagaagg tttgctatgc gtagtttatt tgtgaaaaaa tacactttat
    46381 cgttttgtat agcacaaaaa tcattgctat gtaagcgttt ttacttacgg taaaaatgat
    46441 tatatacgta attcagatag ttatctatca tgaagtcata agcagcaaac attcacaaaa
    46501 ctaaggaggg gcttatggaa agtgtaaaga aacaagaacc atttcaactg acgctatcga
    46561 aaaaagaaga aaagctcaga agacgaaaaa aaataattgc aggaatcaaa acgaataaat
    46621 ttttatatgt gatgattctg ccgggagtcc tttatttctt gatttttagg tatcttccta
    46681 tggggggact cgttattgct tttcaagatt atcaaccatt tttaggaata atgggaagtc
    46741 cttgggtcgg tttcaaacat ttcattcgtt tgtttacaga accgacattc tttacattat
    46801 taagaaatac gttgatcttg ttcggattaa atatcttcgt gttttttccg atgccaatca
    46861 ttttgtcttt actattaaat gaagtaacga acaaacgatt gaaaaacggg attcaaacga
    46921 tcatttacat ccctcacttc atgtcttggg taatcattgt ttcgattact tatgtatttt
    46981 tgaatgttga cggaggggta ttgaatgaat tccttgcaac aatgggattt gaaaaaatca
    47041 gttttctaac atcccctgaa tggacacgca cagtatatat cggacaagtg atctggaaag
    47101 agttgggctg gtctacgatc atctaccttt cagcgataac tgcagtcgat acacagttgt
    47161 atgaagctgc agaaatggat ggagcaggac gcttacgcaa aacatggcat gttacattgc
    47221 cggcaattcg tccagtaatc attaccttgc tgattctaaa aatcggtcag acattagact
    47281 tagggttcga gcatatgtac ttgttattga attcattgaa tcgaagcgtt gcagaaattt
    47341 tcgacaccta tatttataca gctggattga agaatggaca gttaagtttc agtacagcaa
    47401 tgggattgtt taaaggggtc gtaggattgt ttatggtaat tttagctaat aagattgcca
    47461 aaaagctagg agaagacggc gtttactaag atagaaagag agggaaagat atgctgcagt
    47521 cattagataa aataaacgga aggctaaatc gtattttaca gttggtttat ttgaattttc
    47581 tgtggggcgg tttagtcatc gcaggtttgg gagtttttac tttcggtcct gcatcgtatg
    47641 cgatggttgc agtgattaga aaatggctgc gctcaaaaga aacgtttcct ttgacgaaaa
    47701 cgtacttcac atattttaaa gaaaactatc gtgaaacctt gctgatgagc tggatatatg
    47761 gcgccatcgg gattgtttta cttgtggatc tttctttcat acacaattgg tatcttagga
    47821 tcgctttgta tattgctgcg ttcatatatg tgctctcagc actctatatt ttcccaatca
    47881 tggctcatta tgattggaaa ggatattttt tcaaaatgaa aatggcattt ttattcggat
    47941 tctcacatct tcattactca ttagtgttga tattaagtat ggtagggatt tatctgatga
    48001 tcatccaaac aatgccaggt gttttaacct ttatcggtgg cagtctcttc ttttccgcag
    48061 tcacgtggac agcaaattct atattttgcc atttggaatg gaagaactca tatacagaaa
    48121 aaacagaaga aaacaaaaat attattaaaa cagtgaagga gaaaatttat gaaaaaagca
    48181 acgctcgtaa aattaggtct tagttttgca ggaattctag cacttgccgc atgcggcagc
    48241 agttcaagta acagttcttc agagacagaa gatggaaaag ctaaaatcac ttggttaaat
    48301 attttgcatc atgcttcacc gcctacagac acgattgtga acttgatcga agaaaaaacc
    48361 gatacggaac tgacattcaa ctggattcct gatgcttcaa aagaagaaag attaacaact
    48421 gcattggctt ctgatgaact aggagatatc gttacacttc aaattgcgat gttgaaaaac
    48481 tcttctgtcc gcaatgcgtt aaaatcaggc gtattctggg atgtatcaga atatttaaaa
    48541 gactataaaa atttaagtca aatttctgaa gatagaatcg aagctgcgag tatcgacggt
    48601 gccttgtacg gcgtaccgat acaaaaagac tacgctcgtg ctggcttagt cattcgaaaa
    48661 gactggttgg ataatctagg actagatgtt ccaactacca tggatgagct ttatgaagta
    48721 gctaaacaat ttacagaaaa cgatcctgat ggaaatggtg tggacgacac agttggtttt
    48781 ggcgatcgtg cgcctagtga tattcgttat accagcttta aacttttcac ttcttatttt
    48841 ggagcaccaa atggctgggc tgtagacgat aatggcaaat tcactccaga atttgataca
    48901 gaagagtata tgaaagcttt gaatttttca agagatctat ttgagaatgg ctatttggca
    48961 caagactttg cagttaccca aaagactgat cagcaagaac aatttgcaca aggaagaact
    49021 ggaatctaca ctggaatggt ggatatcaag aatctgaaga cgatgggtca aggtattcaa
    49081 gatgatatgg aattagtgcc tgtaaacaag atctcagatg gtgaaggcgg cggttatcat
    49141 gtttggtcag aaggaaatgg tattggcggc ttgctagcat ttcccaaatc tcaagtaaaa
    49201 gacgaagcac aattgaagaa attacttcaa tttgttgatg atcttttaga agaagatgtg
    49261 tataaattga tgactggcgg tatcgaagga acgcattatg aattgaatga tgatggttct
    49321 atcgaattta tcgatatgga tctttggcaa caagaggtgc aaccgttatc aagctctcgt
    49381 cctagtgaag tgacgttctc tttcaaagat acagaccctg aaaaagaatt ctctaatcaa
    49441 ttgatcaaag aaaatgaaga atttgctgta atggatcctt ctgtgccatt ggattcaccg
    49501 acgaacaatg aagtaggatc agaaattgaa aaaatcgtca ttgatgctac ggtgcaatac
    49561 atcatgggac agcttgacga agatggattc aaaaaagctg tagaagactg gaaagcacaa
    49621 ggaggcgatc gaatcacaca agagtacgag gaagcataca aagcagcggc acagtaatat
    49681 agacgattgg gacaaatact tcttggtatt tgtcccaaat cctattttaa tttttaaagg
    49741 agagcaaata atggaaaact ggtcaaagaa attagcaaaa agtatcatgg aaagaacacc
    49801 tcgcttatat gaagaaaagt ggtataaagg aaaatggtct tatgactatg gcgtcgtact
    49861 taaagggttc caattacttt gggaacgaac ccaagaaaaa atttattttg atttcataaa
    49921 agacaatatc gactatttcg tgcaagaaga tggaacgatc cgtggctaca gcgttgaaga
    49981 atacaatatc gaccatgtaa atacaggaaa gcttttcttt ttattgtaca aagaaacagg
    50041 cgaagaaaaa tataaaaaag cagcagaact cctttcccag cagttggcaa accatccacg
    50101 cacatccgaa ggagcttttt ggcataaaga aatttatcct tatcaaattt ggttggatgg
    50161 cttatatatg ggttctcctt tttatgctga atttatccag aattttagtg aagaaaaaga
    50221 ttaccaagat gttttgagac agtttgaaat cagttataaa catctattcg atccaaaaac
    50281 aaatttgctg attcatgcat gggatgaaaa gaaggtccaa ccatgggctg atccaaaaac
    50341 aggtctatcc catcattttt ggagccgatc gattggctgg tatttgatgg ctgcagcaga
    50401 tacctatgaa ttacttcatg aagaaacaga ttgttctttg ctgaaagaaa ttctagaaaa
    50461 gacactggct gcattacttc catatcaaga agaaaacagc gggacatggt atcaagtaac
    50521 aacagaaaca aaacggaaag gaaattatct ggaagcttcc ggcagtagta tgtttgttta
    50581 cgcaatggct aaaggaatac gtttgggact gctgaacaaa gaagaatggc ttccacaagt
    50641 gaagaaatca catcaaggat tacttgatga atttgttctg gaaacaaaag aaggctggct
    50701 gaacttgaat aaaaactgtc aagtagcagg gttaggcgga gcagatcagc gagatggttc
    50761 atatgcttac tacatcagtg aaccaattat ttgtaatgat caaaaaggcg ttggtgcatt
    50821 cttgcaagca ttgattgaag tggaggcatt ataaatatgt atgatatttt gacatttggc
    50881 gcatcaattg atgaattgaa tactgaagca atacaacaag ccattgatgc tgcagcaagt
    50941 gacggaggcg ggaccgtagt tgttccagca ggtgaatttc taacaggagc tttattttta
    51001 aaaagcaatg tcgaacttca tttaagcgca ggagcggttc tgaaattctc tgacgatcca
    51061 aaagattatc ctgttgttca ttcacgttgg gaaggagtcc atcgaaaagt ttatgcttcc
    51121 tgtatctatg ctcaaaatgt agagaacatc tctgtcactg gtttcggtac attagatgga
    51181 aatggaaaaa aatggtggca tacttttaga aatgaaccag ataatctcgc ttatcctcgt
    51241 cctaaattga tgagtttcca taactgtcat cgaattactg tgaaagatat taaattgatc
    51301 cagtcaccaa gttggacgat caacccgatt ctttgcagta atgcaacgtt tgataacttg
    51361 acgatcttga acccagcgga ttcaccaaat acagacggaa ttgatccaga atcatgcaaa
    51421 aatgtgcgta ttagtaattg ccatattgac gtgggagatg actgtattgc aatcaaggca
    51481 gggactgaag atacgtacga aaggatcgct tgcgaaaata tcacgatcac aaattgcacg
    51541 atggtccatg gacatggcgg tgtcgtatta ggaagtgaaa tgagtggtag tattcgtaat
    51601 attaccattt caaattgtat cttccaagaa actgatcgag gaatacgatt gaaatctaga
    51661 cgaggtcgag gcgggatcgt tgaagatatt cgagtatcca atatagtcat ggataatgtg
    51721 atgtgtccat ttatactgaa tctttattat ttctgtggtc cgcgagggaa agaaccgtat
    51781 gtatgggaaa aaaaggctta tccgatagat gaacggacac cagcattcag aaggatccat
    51841 ttttcgaata tcacggcaag gaatgtccat gcttctgcag gttttattta tggattagcg
    51901 gaacaattca tacaagaaat cacatttgac aatatcgatg tctcgatggc ccaagatgca
    51961 gttcccggtg ttcctgctat gatgacagga atagaggaaa tgagcagaca aggattttat
    52021 attggctttg cgggtaaggt ttcctttaac cagatgtcga ttgaaaatca cgagggacca
    52081 gcatttctat tagaaaatac taaagaaatc agtattgaga aattccaatc aaatcaatca
    52141 gaaaatatgg atgaactggt tgttcgtaaa aactttgctt atgcagttga atgatttttt
    52201 aggtgctcca tctagcagac atacccctag tggaaaacga ttataccgag aaaaacaaaa
    52261 cggttttatg cttgaatact atcaattgta cttaaatgaa ttggaaacag taccagccct
    52321 ctatgcttat ccagaaaaag aaggaccgtt tccgccggtc ctttacctgc attctcacgg
    52381 cggagatttt tcagtcggga aaagtgaatt gatccatggt gctccttact tagcttctcc
    52441 atcttttgca gaagtactga caggaatggg atatgctgtt tggtcgattg atgcttgggg
    52501 atttgaagaa cgcggaggta tttctgaaag cgagttgttc aaacaatttt tattaacagg
    52561 aaaaacgtta tggggaatgc gtatttatga tgtgatcagc ttgatcgatt atttggagac
    52621 aagggaagac acagatactc aacggctcgc aacgattggt ctttctatgg gaggcttatt
    52681 aagctggtgg gtgacagcat tggattctcg tgtcaaagtt tgtattgatc tagcggcaca
    52741 ggtggacatc gaaatacttt tgctacatcg ccgcctagat catcatgggt tttattacta
    52801 tgtgccaaat ctattgacag cttatacaac gttagcaatc caagaaaaaa tcgctccgcg
    52861 ccctcgtctt agcttagttg gaaaaaacga tacgatgtgc ttggacgaag gcgtactgaa
    52921 gctgagaaaa gggctggaca aaaagtatca atcaatgggt agtccagaaa acttttcttc
    52981 tttttcttta acaggcggtc atatggaaac acaagaaatg agaaatattt ggaaacaatt
    53041 cataagggag catttatgag gatgaaagac aaactgataa ttggaaagaa tgctatagat
    53101 accccgacgg attttgaaac tatccaagaa ggaatcgatt atttggcagg cctgccggat
    53161 agccatccaa aggtcttgat agtaaaagaa ggaagatatg atgaatatat agaaagcaga
    53221 ttatccaatt ttaaacttgt gggagcagga acagtagtta tctccggtaa tcgttttgca
    53281 aaacaggagt atcttgacgg tagccagtta gggactttcc gaagtgctac ttttttctta
    53341 gaaggatcga atgtcgtatt agaaaatctt cacatcgaaa atacagcagg tccaggagaa
    53401 aaggtaggac aggctgttgc tttattcaat tcaggagatc gcgtatcttt aaaaaactgt
    53461 cgactatctg gttatcagga taccttatgc acgggtccgt tgcctgattt gcaaaaagat
    53521 ggtactcctt tttgtacacc ggcttctgcc aatcctgagt attgtcgtca gacctatgaa
    53581 tattgctgga ttgaaggaac ggtggatttc atatttggtg gggcagatgc cgtttttcat
    53641 cagtgtcata tccatagctt agccagtaaa aatcctggat acatcacagc agcatctacg
    53701 ccgaaaaatc aaaaaacagg gtatcgtttt gaaagctgta ccttgactgc agaacctgat
    53761 actagaaacg tctatttagg aagaccgtgg cgtccgtatg caaatgtctt atttgaagcg
    53821 tgtaagatag gtgggcatgt tcacccaact ggttgggata attgggatga caagaaaaat
    53881 gaacagactg tcaaattctc tgaacgtaat aacaattatg aaacatcgat caaaagacct
    53941 gaatggatac atttagaaaa gcagggggtt cagaaatgaa aagaaaatgg gaactattgc
    54001 taggaatggt cggtggcagt ctatctttga ttttctttgg cgggttagca gttactttaa
    54061 gtaatatgtc tgcgtcagag tttaaaaaaa gttatcagtc gcttgctgta gatcattcaa
    54121 ctttgtcttt agaaaataca tttggattat tacaggacat gactggtctg tttgccgttg
    54181 tgttgtttat ttcacttgct tttttagcag tagcattgtt tttgactgct aaaggaaaat
    54241 acttaacaac tgccactggt ttgtatttta taacaggatt catattgttg attggcactc
    54301 agtttattgc gtttcctttt gcattttttt atttcgcagc tggggcattc agtttatatc
    54361 gagtaagaat gagaaaagga gcctgaatca tgcagactat caaagtgaaa aatcctattt
    54421 taagaggatt caatccagat cctaatatat taagagtgaa agagcggtat tacattattg
    54481 tctcctcttt tgaatggctt ccaggaatcc gagtatatga gtcgaaaaat ttagtcaact
    54541 ggtcacatac aacggatatt ttgaccactc aagtatcttt gcaaggaaat cctacaaatg
    54601 gaagtatatg ggcgccacaa atcagttatg ctgatggatt gttttatctt gtttacacag
    54661 atgtcaaaag tgcaggccgt ccgtttaaag atgtccacaa ttatttgatc actgcatctg
    54721 agatcacagg tccttggtcc gaaccaattt atttaaatag taacggcttt gatccttctt
    54781 tgttccatga tcttgaaacg ggaaaaaaat ggcttgtcaa tgaactatgg gattatcgtc
    54841 tggacacacc gaataaatcc agcggtatcg tattacaaga atatgatgca gagaagaaga
    54901 agcttgatgg tcccatctat aaaatatttg caggtacaga attagcgaaa acggaggcac
    54961 cacatttgta ttttcgtaac gggtattatt atttgatgac ggcagaaggc ggtacaggaa
    55021 gaggacattc agttacagtt tgccgagcaa agaatattac tggtccttac gagcttgatc
    55081 ctggttttcc tatgctgaca gctagtgaca aacctgcttc tacattgcaa tgtacaggac
    55141 atgggagctt agtacaaacc ccgtcaggaa gatggtacat gacatatctt tgtaccagac
    55201 cattaaacgg tgcggctatt ttgggaagag aaaccgctat tcaagaagtg tattggacgt
    55261 ccgacgattg gttaagactt aaagatagcc agacaactcc gatggaagaa ataatgattg
    55321 agacaaatga agaggtacaa caagaaaaag atcatcagtt tatggataca tttgaaggag
    55381 agctaaagaa gaactggaat gccagacgca ttttgccaaa tgctgattgg tgtgatacaa
    55441 aatgtagacc aggtttttta cgtctgattt ccggtgaatc tcttcaatct accttcgaac
    55501 atcatttgtt agctattcgg caaacggatt ttacttttga tgcggagaca gcatttgaat
    55561 acatgcctaa taattttaat cagatggctg gactggtact gtacttaaat gaatccaatt
    55621 atatttattg ttatgttact tggaatgagg acaagggaaa atgcctacgt atgatagaat
    55681 cagaaaacgg agtagcacaa atagaagatt ggacgattcc actcacagaa aagaagactt
    55741 ttttgaaagt cactgtaaag aaagagacag cacagtttca tttgaaagac gtatcaactg
    55801 aagaatggaa gaaagtaggt ccgcaaaaaa acatgtgtat actctcagga gggtttactg
    55861 gaaattttat cgggatagca gtgcatgatc taaataaaaa aagaggaagt tatgctgatt
    55921 ttgagttttt caagtatgat ggaaaagatc atctgtaatt taattcttta ataaatgagg
    55981 ttcactaact tattttcact agttagtgaa tttttttgtt tgtatatatt gttatgtaaa
    56041 aaaagttatc aatcttatgc atcaatgttt ttagattatt ttttgttttt tatctgtcaa
    56101 gataatgcaa taaaaagcca acattgtgaa tataaaaaca agaaaaaaag agttataatt
    56161 ttgaaaacgt ttactagaag aggtaggagg aattcaaatg ctttcacaaa atgctgttac
    56221 agcaaataca ataaaaaaag aaaaaaagga atttgggatt tccgataaga taggctacat
    56281 gttcggtgat ctaggtaact gttttatttt aggtttagtc aatagtttct tgatgattta
    56341 ttatacgaat gttcttggcg tttcaggggc aatcgtaggt acgttattct tgatttcgag
    56401 aattttggat gcgtttgctg atgtgatggt tggaagatta tgtgatgtct cagagttaac
    56461 agaaactggc cgatttattc cttggatcag aaaaatgaag tatccatttt gcttagtgac
    56521 tgtcattttg tttttgccat tcgtccaaca tcttccaatg acggtgaaga ttgcttatat
    56581 ttttgtttca tacattgttt atgggatttt tctctcaaca atcaatatcc cgtatggagc
    56641 aatggcagca gcaattagct caaatcctaa tgaccgcacc tcgttgtcca cgttccgaag
    56701 cattggttct gcgattggcg gatcaactac aggattcttc attccgattt taatgtatac
    56761 ccataacaag agtggacaac aagtcgtttc agggaatcat ttcttttgga tttcaatcgg
    56821 atgtgctgca atagcatttg tcgcatatat gttaacttgt aaattgacta ctgaaagagt
    56881 aagagttgtc aaaaacgaaa aagtatctgc ttctcagttg gtaaaaggtt tgttagctaa
    56941 tcgagcactg attattttag tcattgttga cattttcatt gtgatcaatc agattttagc
    57001 tgggacgaat atgacttatt tattcaatga ttacttccat aataaacaag caatgtctgt
    57061 tgctctttta tttacttacg gaacagtagt agttttagca ccatttgcta caaagctgac
    57121 aaaattattt ggtaaaaagg aagcaagcgt atttgcgtta cttgcttctt catgtatgta
    57181 cttgctgatg tatttccttc atataacaaa tgcatgggta tatctgattc ttttatttat
    57241 cgctacttta ggatctggtt tattcaattt gatggtctgg gcattcatta ctgacgtgat
    57301 tgattatcat caatataaga caggcttaag agaagatgga acagtatatg gagtaaattc
    57361 ttttgcgaga aaagtaggac aagcatgtgc aggagcggta ggcggattta tgctggcact
    57421 cattggttac caatcgtcta caacaggtgg agccatccaa tcagttttag tacaagaaag
    57481 gatttatacg atggcaaact tgttaccagc attctgctta tttgtatccg caatcatttt
    57541 gctagtaggt tacccactga atcgttcaaa aactgtattt attggtgaag aattgaaaaa
    57601 aatcaatgaa agatagggag aaacgtattg cggacaaaaa aagggattat caatgctttg
    57661 aactttaaaa caaacgacac tcaacaagat gatacaataa tactgcaaaa agcaatcgat
    57721 caaggtgcga aagaacactt acctgtattt attccaaaag gcatttattt agtcggagcc
    57781 ctttttttaa aagataagag tcatcttatc tttgaagaag gtgcagtcct caaggggaga
    57841 acagatatcg aagcatttcc agaaatcgat acacgagtag ccggcgtaga aatgaaatgg
    57901 ccagcagcga ttttgaatgt tctctcggcg aaagatattc ttatagaagg aaaaggaata
    57961 atcgacggtc aaggagacca ttggtgggag ctgtattggg gaaaagatca aaaaagcggg
    58021 acaagagctg aatacgatca aaagggactt cgttggattg cggattatgc tattaaacgt
    58081 ccacgcgctt gtttactgta tcatgcagaa catgttgttg ttcgtgattt gatttttcag
    58141 aaatcaggtt tttggaactt gcaaatcact tattctaacg atgtattagt agaaaaagta
    58201 atcatccgca acaatgatgg accaagtaca gatgggattg atattgattc ttcaacaaat
    58261 gtgcgtgtct atgaatgtga tttggcctgt ggtgatgatt gtattgctat taagtctggc
    58321 agagatggca atggtgcaag agtcaatcga aaatcatcaa gaatcgaggt tgctagatgt
    58381 aaaatccgtt caggatacgg tgtaactatt ggtagcgaag ttagtgctgg agttagtgac
    58441 gtatatattc acgatatcga tttttttcag tcagactgtg gtattcgaat gaaatcttca
    58501 aaagaaagag ggggagtgat cgaaaatatc agggtagaaa atctgaatat gattgatgta
    58561 caatttcctt tttcttggat aatggactgg cataatgaat acaatagaaa aaatagcaat
    58621 gaccttgctc aaatgccaga aagttggcaa gcagtggctg aagaaatacc tgaatcaaag
    58681 caaatgacga aagtcaaaga catacacatc aaaaacgtac aggcatgcct ttctccaggt
    58741 tactcacttc cttcccgagc ttttgattta gtagcatttc ctgaaaagcc tatagaagat
    58801 gtctgctttt ctcattgcca aataactgca aaagaattcg ggcgaatcga agcagtacaa
    58861 aacttatgtt tcaaaagctg tgatttatct atagaaactg gcaacacagc tgcaaacaat
    58921 acttttgaca atcgttgaaa ttataataag gtcaagctta aataatattt tagcttgacc
    58981 tttatttttt ttaatgatat attaaacaac gttgtcgcgc ttcattgaaa cattttatga
    59041 aaattccaaa aaagagttga cacatgtttt tagacgtgat aagataacta agtcgttaaa
    59101 aagaaccact tgcgaagctt gattctatcg aaaaaaactt caaaaaagtt cttgacaaat
    59161 aacaaacgat aagttatgat aaatgagttg ctgaaagatt tcgaaaaacg aaatcgaaaa
    59221 aaacttttaa aaaagagttg acaatcgttt gtctctctga tatgatataa aagttgctac
    59281 ggcagccaaa aatgaatcgg gaaacactct cgatgaagta gacctttgaa aactgaacaa
    59341 agtaagacaa accaaatgtg tagggcgtct tgattcaatt caagacaa
//
LOCUS       DACCYH010000007        55305 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.007, whole genome shotgun sequence.
ACCESSION   DACCYH010000007 DACCYH010000000
VERSION     DACCYH010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.007"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            13..870
                     /locus_tag="IVY85_000407"
     CDS             13..870
                     /locus_tag="IVY85_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP8043446.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            985..1905
                     /gene="pstC"
                     /locus_tag="IVY85_000408"
     CDS             985..1905
                     /gene="pstC"
                     /locus_tag="IVY85_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP8043447.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1905..2789
                     /gene="pstA"
                     /locus_tag="IVY85_000409"
     CDS             1905..2789
                     /gene="pstA"
                     /locus_tag="IVY85_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP8043448.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2804..3610
                     /gene="pstB"
                     /locus_tag="IVY85_000410"
     CDS             2804..3610
                     /gene="pstB"
                     /locus_tag="IVY85_000410"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8043449.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3626..4384
                     /locus_tag="IVY85_000411"
     CDS             3626..4384
                     /locus_tag="IVY85_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8043450.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4397..5074
                     /gene="phoU"
                     /locus_tag="IVY85_000412"
     CDS             4397..5074
                     /gene="phoU"
                     /locus_tag="IVY85_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP8043451.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5312..5611
                     /locus_tag="IVY85_000413"
     CDS             5312..5611
                     /locus_tag="IVY85_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043452.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKK"
     gene            5637..6344
                     /locus_tag="IVY85_000414"
     CDS             5637..6344
                     /locus_tag="IVY85_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8043453.1"
                     /translation="MSIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLL
                     NISILFVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYE
                     ELNYLTRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQV
                     GRDVIGSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVL
                     SIPISVLFFLTVRRLKR"
     gene            6341..7108
                     /locus_tag="IVY85_000415"
     CDS             6341..7108
                     /locus_tag="IVY85_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8043454.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7208..8776
                     /gene="liaX"
                     /locus_tag="IVY85_000416"
     CDS             7208..8776
                     /gene="liaX"
                     /locus_tag="IVY85_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP8043455.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9076..9393
                     /locus_tag="IVY85_000417"
     CDS             9076..9393
                     /locus_tag="IVY85_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP8043456.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            9393..9743
                     /locus_tag="IVY85_000418"
     CDS             9393..9743
                     /locus_tag="IVY85_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP8043457.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9910..10848
                     /locus_tag="IVY85_000419"
     CDS             9910..10848
                     /locus_tag="IVY85_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP8043458.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10863..11693
                     /locus_tag="IVY85_000420"
     CDS             10863..11693
                     /locus_tag="IVY85_000420"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP8043459.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11737..12762
                     /locus_tag="IVY85_000421"
     CDS             11737..12762
                     /locus_tag="IVY85_000421"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8043460.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12777..13715
                     /gene="galU"
                     /locus_tag="IVY85_000422"
     CDS             12777..13715
                     /gene="galU"
                     /locus_tag="IVY85_000422"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8043461.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13840..14280
                     /locus_tag="IVY85_000423"
     CDS             13840..14280
                     /locus_tag="IVY85_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP8043462.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14283..14885
                     /locus_tag="IVY85_000424"
     CDS             14283..14885
                     /locus_tag="IVY85_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8043463.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(14953..16059)
                     /locus_tag="IVY85_000425"
     CDS             complement(14953..16059)
                     /locus_tag="IVY85_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8043464.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16269..17270
                     /gene="ccpA"
                     /locus_tag="IVY85_000426"
     CDS             16269..17270
                     /gene="ccpA"
                     /locus_tag="IVY85_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP8043465.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17573..19918)
                     /locus_tag="IVY85_000427"
     CDS             complement(17573..19918)
                     /locus_tag="IVY85_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8043466.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20197..21099
                     /locus_tag="IVY85_000428"
     CDS             20197..21099
                     /locus_tag="IVY85_000428"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP8043467.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21312..23087
                     /locus_tag="IVY85_000429"
     CDS             21312..23087
                     /locus_tag="IVY85_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP8043468.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            23344..24561
                     /locus_tag="IVY85_000430"
     CDS             23344..24561
                     /locus_tag="IVY85_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8043469.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLCSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFLNRLTPYALILIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKEALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(25008..26594)
                     /locus_tag="IVY85_000431"
     CDS             complement(25008..26594)
                     /locus_tag="IVY85_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP8043470.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            26845..27069
                     /locus_tag="IVY85_000432"
     CDS             26845..27069
                     /locus_tag="IVY85_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP8043471.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            27211..28962
                     /locus_tag="IVY85_000433"
     CDS             27211..28962
                     /locus_tag="IVY85_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8043472.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            28962..30746
                     /locus_tag="IVY85_000434"
     CDS             28962..30746
                     /locus_tag="IVY85_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8043473.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            31204..32055
                     /locus_tag="IVY85_000435"
     CDS             31204..32055
                     /locus_tag="IVY85_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8043474.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            32152..34038
                     /locus_tag="IVY85_000436"
     CDS             32152..34038
                     /locus_tag="IVY85_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8043475.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(34079..34981)
                     /locus_tag="IVY85_000437"
     CDS             complement(34079..34981)
                     /locus_tag="IVY85_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8043476.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            35211..36614
                     /locus_tag="IVY85_000438"
     CDS             35211..36614
                     /locus_tag="IVY85_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8043477.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            36614..38047
                     /gene="ascB"
                     /locus_tag="IVY85_000439"
     CDS             36614..38047
                     /gene="ascB"
                     /locus_tag="IVY85_000439"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8043478.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            38192..39118
                     /gene="trxB"
                     /locus_tag="IVY85_000440"
     CDS             38192..39118
                     /gene="trxB"
                     /locus_tag="IVY85_000440"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP8043479.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            39331..41058
                     /locus_tag="IVY85_000441"
     CDS             39331..41058
                     /locus_tag="IVY85_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP8043480.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            41131..41424
                     /locus_tag="IVY85_000442"
     CDS             41131..41424
                     /locus_tag="IVY85_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8043481.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAIIQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(41508..42368)
                     /locus_tag="IVY85_000443"
     CDS             complement(41508..42368)
                     /locus_tag="IVY85_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP8043482.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            42460..42888
                     /locus_tag="IVY85_000444"
     CDS             42460..42888
                     /locus_tag="IVY85_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8043483.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            43050..43226
                     /locus_tag="IVY85_000445"
     CDS             43050..43226
                     /locus_tag="IVY85_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP8043484.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            43253..43699
                     /locus_tag="IVY85_000446"
     CDS             43253..43699
                     /locus_tag="IVY85_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP8043485.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(43855..45234)
                     /gene="brnQ"
                     /locus_tag="IVY85_000447"
     CDS             complement(43855..45234)
                     /gene="brnQ"
                     /locus_tag="IVY85_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8043486.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            45940..46911
                     /locus_tag="IVY85_000448"
     CDS             45940..46911
                     /locus_tag="IVY85_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP8043487.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            46935..49127
                     /locus_tag="IVY85_000449"
     CDS             46935..49127
                     /locus_tag="IVY85_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP8043488.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            49144..49620
                     /gene="ybeY"
                     /locus_tag="IVY85_000450"
     CDS             49144..49620
                     /gene="ybeY"
                     /locus_tag="IVY85_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP8043489.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            49598..49999
                     /locus_tag="IVY85_000451"
     CDS             49598..49999
                     /locus_tag="IVY85_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP8043490.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            50014..50913
                     /locus_tag="IVY85_000452"
     CDS             50014..50913
                     /locus_tag="IVY85_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP8043491.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            51056..51868
                     /gene="recO"
                     /locus_tag="IVY85_000453"
     CDS             51056..51868
                     /gene="recO"
                     /locus_tag="IVY85_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP8043492.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            52252..53169
                     /gene="glyQ"
                     /locus_tag="IVY85_000454"
     CDS             52252..53169
                     /gene="glyQ"
                     /locus_tag="IVY85_000454"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP8043493.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            53171..55246
                     /locus_tag="IVY85_000455"
     CDS             53171..55246
                     /locus_tag="IVY85_000455"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP8043494.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        17912 a         9928 c        11772 g        15693 t
ORIGIN      
        1 aggagatttc taatgaaaaa acttaaatta ttcttaccaa taataggatt agccattctt
       61 ttatcaggct gttcaaagtg gattgatcga ggagaatcca ttacggctgt tggctcttct
      121 gctttacagc ctttagtaga aacagttgct gaaacttacc agacagaaaa tcctggaaaa
      181 ttcatcaatg tacaaggggg cggaagcgga actggtttgt ctcaagtaca gtcaggagca
      241 gtcgacatcg gaaactctga tttgtttgcg gaagaaaaat cggggatcga tgcctcagca
      301 ttagtcgatc atcgagtagc agttgttggg atcactccta ttgtgaacaa ggatattggc
      361 gtaaaagaca tcagtatgga aaacctgaaa aaactattta caggcgagat caaaaactgg
      421 agtgaagtcg gtggaaaaga tcagccagta gttatattga accgcgcttc aggaagcgga
      481 acccgtagca cgtttgaaaa atgggtattg gacggagaaa cagctgttca ggcacaagaa
      541 caagattcaa gcggaatggt gcgccagatc gttgccgaca cacccggtgc aatcagctat
      601 gttgcttttt cttatgtttc cgatgatgta gatacgttga atgttaacgg tgtagaaccg
      661 acagatgaaa atgtgacaac gaacaaatgg acgatttggg catatgagca tatgtacaca
      721 agagatcatc caaaaaaact gacaaaagaa tttttagact atatcttatc tgatgaagta
      781 caagataaca tcgttggaga actaggttat atccctgttt ctcatatgaa agtcgaacgt
      841 gattggcaag gaaatatcgt taaagagtaa tctttacaca aaatttacaa acttagctaa
      901 acttttacat gttattaaaa ccaaattgat gttcaatttg tacactgagt gagtatgtta
      961 aacctgatta agggggagtc atctgtggaa gatgtgaaaa aagtattatt aacaaaatca
     1021 aaacgttcaa aaatggaaca gcgtggaaaa ttcatcagtt ttttatgtat cgcgctgatc
     1081 gtgttggttg tattatctat tttttatttt gtagctagta aaggattggc cacctttttt
     1141 actgataaga tcaatgtatt tgattttctg tttggtacag attggaatcc aagccaaatc
     1201 ggagcaaatg ggaaaccaat ggtaggggca ttgcctatga tcacaggttc gtttattgtg
     1261 acctttttat ctgctatcgt tgccacgcca tttgccattg gtgcagcagt attcatgacg
     1321 gagatctctc ctaaaaacgg aacgaagatc ttacagccgg tcatcgagct gcttgtaggt
     1381 attccttctg tcgtttatgg ttttatcggt ttatcagtta tcgtgccttt tgtcagaagt
     1441 atttttggcg ggacagggtt tggtatctta gctggtacat ttgtgttgtt cgttatgatc
     1501 ttgccgactg tcacaacgat gactgttgat gcattaaagg cagtgccacg gcactatcgg
     1561 gaagcttcat tagcgcttgg tgccacaaga tggcaaacaa tctacaaagt cgtgctacga
     1621 gcagccgtgc ccgggatttt aacagcagtc gtttttggta tggctcgtgc gtttggtgaa
     1681 gccttagcta tccaaatggt catcggtaat gcagcattga tgccaacagg acttactact
     1741 cctgcttcta cattgacgtc catcttaaca atgggaatcg gaaatacaat catgggaaca
     1801 gttgaaaata atgtgttatg gtcattagcg ctgatcttgc tattgatgtc attattcttt
     1861 aatatcgtga tccgtatgat tgggaagaaa ggagagctga aataatgaac gcaaaacgct
     1921 cagataaaat tgctacaggt gttttatata tagtttcggg gatcatcgta ttgattttag
     1981 cagctttact actttatatc ttagttcggg gaataccgca tatttcatgg tccttcttga
     2041 ccgaaccttc caaagcctac caagaaggcg gtggaatcgg gatccagcta tttaactcga
     2101 tttatttgct tttgatcacg atgatcatca gttttcctat ttctttaggt gcgggtatct
     2161 atctttcaga atatgctgga aaaaactggc taacagatgt gattcgcacg tcgattgaaa
     2221 tcttgagctc gttgccttct gtcgttgtcg gtttattcgg tttcttggtt ttcgttattc
     2281 aattccagta tggtttctcg attatttctg gtgcattggc tttgactgta ttcaacttgc
     2341 ctcttttgac tagaacagtt gaggagtctt tacaagcggt ccattacaca caacgagaag
     2401 caggattggc cttaggtttg tcacgctggg aaacagtgat aaaagtggtg gttccagaag
     2461 cgacaccagg gattttgact ggtgtgatcc taagttctgg aagaattttt ggtgaagcag
     2521 ctgcattgat ctatacggct ggacaaagtg cacctgcgtt agattttacg aactggaatc
     2581 cattaagtgt ttcaagcccg atcagcattt tccgtcaagc tgaaacatta gctgtccata
     2641 tttggaagat caatacagaa ggcacgatgc ctgatggtgt ttctgtttcg gctggcgctt
     2701 ctgccgtgtt gatcattgct gttttactgt tcaacttcgg cgctcgtgcg attggcaaac
     2761 gattataccg aaaaatgact tcagcctaag gagaagaagc aaaatgaaaa aatataactg
     2821 gaatgaaaca aatgtcatca ccatgccaaa agagaaagaa gtagcactat atacggatga
     2881 tctacatgtc tggtatggcg acaatgaagc aatcaaagga gtggatctgg aatttgaaaa
     2941 aaataagatc actgcactga ttggtccttc cggctgcgga aaatctacgt atcttcgttc
     3001 gttgaatcga atgaatgatg ggatcgctaa tacgaaaatc actgggaaaa tcatgtatca
     3061 aggcgtagat attaatgcgc ctcaagtgga tgtttatgag atgcgcaaac ggattggcat
     3121 ggtttttcaa cgtcctaatc cgttcagcaa gtcgatttat gacaatatta catttgcttt
     3181 gaaacagcac ggcgaaaaaa acaaaaagaa attagatgaa atcgttgaaa caagcttgaa
     3241 acaagctgcc ttgtgggacc aagtaaaaga cagcttggac aaaagtgcct tggctttatc
     3301 tggcgggcaa cagcaacgtt tatgtattgc tcgtgcgatc gcgatgaaac ctgatatctt
     3361 gctgctagac gagccggcaa gtgctttgga ccctatttcg acaggaacgg ttgaagaaac
     3421 acttgtaaac ttgaaagaag actatacgat catcatcgtg acgcataaca tgcaacaagc
     3481 agctcgtatc agcgactata ctgccttttt ctatctagga aaagtaatgg aatatgacaa
     3541 aactaacaag atatttacca gaccaaaaat tcaggcaacc gaagattatg tgtccggtca
     3601 ttttggttaa gacggaggcc tcaacatgac aaaagaaatt atttcatcta aagatcttca
     3661 tctgtattat ggaaagaaag aagcactaaa agggatcaat ctaagtttca atcaagggga
     3721 aatcactgcg atgatcggtc cttctggttg cgggaaatcc acttatctgc gttctttgaa
     3781 ccggatgaat gacctgattc cgaatgtaac gatcaccgga agtgttttat ataaagacaa
     3841 agacatctat ggtccaaaaa cagatgttgt tgaactaaga aaagaaatcg gcatggtatt
     3901 ccagcagcca aacccatttc ctttttctat ctatgaaaat gtcatctatg gattgaaatt
     3961 aaaaggggaa aaagataaaa aagtccttga tcaagtagtt gaagaaagcc tgaaggcagc
     4021 gtctgtttgg gatgatgtga aagacaaatt gcataaaagt gctttatctt tatccggtgg
     4081 gcagcagcag cgtgtatgta tcgcacgtgt tttggcagtt aatccggaaa tcatcttatt
     4141 ggatgaacca acaagtgcgc ttgatcctgt ttctactggg aaaatcgaaa gtatgctgtt
     4201 agaattaaga gagcggtata cgatgatcat cgtgacccat aatatgtctc aagcttcacg
     4261 tatttcagat aagacggcat tctttttaga tggtcacttg atcgaataca acgatacgaa
     4321 gaagatattc atgaatccag aaaaacaaga gacagaagat tatatttcag gtcgatttgg
     4381 ataaggagga aacggaatgc tacgtacaca atttgaagaa gaattgctga atctccataa
     4441 tcagttctac gaaatgggga tgatggtgag cagcgccgtg cacaagtcag ttcgtgcata
     4501 catcaaacac gataaaatct tagcccaaga agttatcgaa aacgatataa atatcaataa
     4561 catggaaaca cgcttggaga agaaaagttt tgaaatgatc gcattgcaac agccagttac
     4621 aactgatttg cgtatgatca ttacggtcat gaaagctagc tcggatttag aacgtatggc
     4681 cgatcatgcg gtgtctgttg ctaaatccac gattcgcgta aaagggcaga cgagaatacc
     4741 ggaaatcgaa aaagagatct ccgatatgtc cgattatgtg aaaaaaatgg tggacaacgt
     4801 cctagttgct tatgtcaaaa cagacgaaaa agacgcacgt acgatcgcca atatggatca
     4861 aagagtcaat gactatttca atcgaatcta taatgcaaca atcaaaaata tgcaggaaaa
     4921 tcctgagaca gtcatcagtg gaacagacta cttgaatgtt gccagttatc tagaacggat
     4981 cggagactat gtgacaaaca tctgtgaatg gatcgtctac ttggctacag ggaaaatcac
     5041 tgagttaaat acgaaccaca atgagatttt ctaaactgaa gctgtgatta tgccgtttgt
     5101 ttaggaaaag aggttgtgaa atcagcggtt gtgaaaaagc tgttctgcat caagtaataa
     5161 gaacagtcaa acaaaaaata gctcctcata tttttcgttt ggctggactt attaccgcag
     5221 atgcaagctt ttgaacaccg tttattcggg aaagggtttg tgacaaaaca tgtcacaaac
     5281 ccttttcatc atttcataga aaggataaag aatgcaaaaa aaaattagca tcatcactat
     5341 attttttatt tgtgtgctta cttcatgggt tattcagcat caagctgctt tatatgaagt
     5401 acctgtcatc gagatcgaaa aagtacgaat aaaagaggat catcaattga ttacaggaag
     5461 actgatgaac acggatcaaa aaggagaatc gatttattta tcggttcctt atcaaaaaaa
     5521 tcaaatagaa acagcaaata taaaagcgag acaaatgtat ctagtccaaa tgcatggaga
     5581 tgaatggcag ctagtagcaa aaaaaaaatg atggctggct tttcttcttc acaagtatct
     5641 ccattggcgc catgttgctt attggcggaa aacagggtct atttgcttta ggaagtctca
     5701 tcatcaatat cgttttactt ttacttttgt tgctcatctt tactaaaaca gaagggattt
     5761 ctctattaaa tatatctatc ctgttcgtcg ttatagggat gattattagc atattggctt
     5821 tagatggatg gaatagaagc agtataatca aaatttcagc tgcagttacg agtgtttttt
     5881 tagcattttt tatttgtctt ttgacaatga atcattggaa agaccaagga ctgcgctatg
     5941 aggaactgaa ttatctgaca cgaccgtatc gttctgtttt tctttctagc gtactgattg
     6001 gaacagctgg aggaacactc gataccgtta tcacagtcat tgcgacatta gaagaattga
     6061 ctaagcgaca accagaaata acagcgaaac agttatggct ttcaggaagg caagtgggtc
     6121 gagatgtgat tggctcgatg accaatatcc ttttgttcgt ctatatcagt gggtcagttc
     6181 catcgcttct tttatattta ggaaatcaat ggtcgttcaa agaaacgata gaacagcatt
     6241 tatcattgga atttttacgc gtcattgctg gcagcttagg aatagtatta tcgatcccta
     6301 tctcggtcct cttttttctg actgtaagga ggctgaaacg atgaatgtac ttattatact
     6361 tgttgggatt ttagcaatca gcgtgctact tattggcggt acgcaaggta tgtatattct
     6421 tttaggctta ttcatcaatt tagggatctt tttcctgttg ttattcggtt atcatcagaa
     6481 gtggccgata ctggcattgt cggtcatagg atttttattg attgctgtgg ttatcttatt
     6541 ttttatcaac ggttataatc taaaaatgcg tgcagctttt gtgagtatac tgatcttttt
     6601 attttgtttc ctactattgc ttcctatcac tgatttttta gcgatccaag gttttacatc
     6661 ggtagaacta gaagaattat ctggcttaga taagacttta gcgattgatt ttagattact
     6721 tacgagatcg ttgcttctga ttagtcttag tggtgctgtt ttagatgcta gtgtagcaat
     6781 ttctagtggg acattcgaag tatatcaggc aaatccgcaa ttatcttttt accaattgcg
     6841 ccattctagt ttcgctattg ctaaaaaaat attggcgtca acagtaatga cacttctttt
     6901 tgcattcatg ggaagttctt tagcgttgat cctctggttt attgatttag acattccttt
     6961 tcagcaagtg atcaatgaaa aatcatttgt tctagaatat acgatggcta ttcttaccac
     7021 attgtctgca ctacttgttc ttcctttaac ctgcgtgact acagcatatc tctttacaaa
     7081 aaagaaagaa caactcaaga tggagtaaaa gttacaactt aagtcctatg acatttttac
     7141 tgaatcagtg cataatatgg atgtaaggaa aagcttgcaa acaattttgc ttaaggaggc
     7201 aatttccatg aaagaaagag aacgtatttt agatttagtg aaaaaaggaa tcctttcgac
     7261 tgaagaaggc ttagatttat tagaaagcat ggcaactgaa aaagacgaaa agcaaataaa
     7321 aaaagaagca gacaaggtaa ctgctagtca taaagaaaaa gatcaggcaa gtcaattgat
     7381 cgataaactt gaaaacggcg aagaagaaat ttctgagcca gttgatccaa aggaaagaga
     7441 aaggcaagat caagaaaact tagaaaagat cttagatgaa ctagcaactg aagcgaataa
     7501 aacttcggcg cgtctagatg aagtaaatgc tcaattcgct gacaataagg cagcacgcaa
     7561 tgagaaacag gaagcattga tgcagctgaa tacaaaagaa gaattaggcg agttgacaga
     7621 agaagaatta gcacagcgtc agacgttaga agcagaaatc aaagaattag aagctagtgg
     7681 cgatacactg ttagaagaac aattgaaatt agaagcagaa ttaaaagata tccgtaagaa
     7741 tcaatggtct gagaaaaaag aaacgtttac tgataagttt gaattgccag atgattggaa
     7801 agatcaagca acagatacac tgaatcaagt aggcgaaaaa atgtcagaag ctggatcaca
     7861 gttaggcaaa ttcttgaaga aaacattcca aactgtttca gaaacagtga atgacaacat
     7921 ggaatggaaa gatgtcagct tacgtgtccc tgggattgcg acaacgaaat ttgagcatga
     7981 attttactat gaagctcctg cagcaagtat ccttgatatc aaggcagcaa acggaaacgt
     8041 gacgttaaaa acgtgggaca gcaacgatgt gaaagttgaa gctaagatca aactttacgg
     8101 aaaaatgggt gctgaaccat ttgaagcatt ctctgaacgg agccagattg aggttaatga
     8161 agatcatatc tccttccaaa ttccaaataa acgtgtgcgt gcagaccttg ttttctactt
     8221 acctaaacga gtatacgacc acgcagctat taaacttcta aatggcaaca tcatgattga
     8281 aacattggaa gctaaagata tttatacaaa atctacgaat ggtaacatca tcgtggacca
     8341 attaactgca acgatgctgg aagtagaagg tgtaaacggc aatatcgata ttcgaaacgg
     8401 gaacatctta gattctatta tcgaaacagt aaatggtaca gtgacatttg gtgcgacagc
     8461 agaaaacctt tctgtgtctt tggtaaatgg tgatgtacgt cttacgatta aagaagataa
     8521 tttgaaaaaa gtagaagcaa gttcagtgaa cggaaatgtc aaagtcgctt taccagatgg
     8581 catcggtttg gaaggacatg ctaaaacaag tcttggaagt atcaacagcc gtctgtccaa
     8641 ttacgaagta gttcgtgaga aaaaagaacg gacaaaccaa atgttgcaat tccgtcgtgt
     8701 ttcagatggt gaaatcacgc aagtgcaact ttcaacgaca acaggaagta tctatttaaa
     8761 agatacagat aaataaagag gttgtgaaag agatgttcag ccttgagcaa taagaagcga
     8821 ttttgtaaaa tagcttcccc tattttacaa aattgtgcct tattgccgat aggctatctc
     8881 ttgaacaccg tttaccaaga gattatgaac ctgccaatta tcgccgagtc acaaggaata
     8941 atttcagaaa aagctcgtca cgttttatga atatggtaat ttgtgaccga gaattttgca
     9001 gaaagacacc gattaagaag aaggttgtca caacgatttt cttcatttaa aatagtagaa
     9061 cagaggtgag acatgatgaa aaaaaagttg acaaaatcca ataaaaatgt tgttttgaca
     9121 gggactttag caggaatagg agaatacatc gggattgatc cgactgttat ccgtgtgctt
     9181 tatgtattcc tgagcttagt tgcttttgga agtccaatca ttttatacat tttattagct
     9241 ttgattatcc catctgcgcg ttctgcacga acaaactatg gacataacaa tgcatattat
     9301 gaaaaaaaca attatcgtga acaaactcgt gaacaaacaa aaaaacgcaa agaagcggaa
     9361 aaaatcgatg atgacgaatg gagtgacttt taatgtcgta ctttcaacgt attttagtta
     9421 atacgttaac attcatatca ttggctgttc tttttcctgc gatggtccat gtgagcagta
     9481 tttggacggc cgttcttgcc agcttcgtat tgtctgtgtt gaatatgttt gtcaaaccga
     9541 ttttgacgat actgtccctg ccctttacat tagtcacatt gggattgttt acgtttgtta
     9601 tcaatgcagc gattcttaaa atgacctcct tttttgtggg agcagaacat tttagttttt
     9661 cttcattcgg tgcagcgttg ttagtagcta tcttgatgtc gcttgtcaat gtaattgtca
     9721 gtgaacataa cagtagaaaa taaaagaaag ctgtgccgaa ctatcggcgc agttttttta
     9781 tttagaatca ggaatgcgtt ttctattaca ttggtaatta aataaaaacg ttttttttaa
     9841 ttggtagtac ttttggctag acaatgataa aatgatagag aacactctaa tagaaatgag
     9901 gtagcatgaa tgtcagaagt tgtaaaagtt agccaattag tgaaaaaatt atctttggaa
     9961 atcgtgacag gcgacgagaa aagcttagac cgtgtcatcg taactgggga catctcacgc
    10021 ccaggactag agttgactgg ttattttaat tattattccc ataatcgttt acagctattt
    10081 ggcagtaaag aaatcagttt tgcggaacgg atgatcccag aagagcgttt gatcatcatg
    10141 agaagattat gtagtgaaga tatgcccgca tttattattt caagaggatt accggctcca
    10201 gaagaaatga tccaggcagc aaatgaaaat gggatagctg tactgcgttc tcccatctca
    10261 acttctcgtt tattaggaga attatccagt tatttagatg gtcgccttgc ccctcgaaca
    10321 agtgtccacg gtgttttagt ggatgtttac ggattaggtg tactgattca aggtgacagt
    10381 gggataggaa aaagtgaaac agcgttggag ttgatcaaac gcggtcatcg attgatcgca
    10441 gacgatcgag tagatgttta tcagcaagat gaattaacgg tgatcggaga accgcctaaa
    10501 atcctggaac atctaattga aatccgagga atcgggatca ttgatgtaat gaatcttttt
    10561 ggtgcgagtg cggtacgtgg ctctatgcaa gtccagttgg ctgtttatct ggaagcatgg
    10621 gcaaaagata agaaatacga ccgtctgggc tctgaagaca cgaccgttga aatcgcggaa
    10681 gtgggcattc cacaaataaa aatccctgtc aaaacaggac gaaacgttgc aatcatcatt
    10741 gaagtagccg cgatgaattt ccgtgcacgt acaatgggct ttgacgcaac gaaaacattt
    10801 gaagaaagat taagtcgctt gattgaagaa aactcaggca acgactagca aggagtgaca
    10861 atatgttagc acaaatcaat cgagttgctt ttgagcttgt agggattcct atctactggt
    10921 atgcattgat catcgtatca ggtatcatta tcgccatgtg gcttagcagc agagaggctg
    10981 ttcgggtagg attaaaagca gatgatgtga cagattttat gttgtgggga ttgcctatct
    11041 ccattattgg tgctcgctta tattatgttt tatttgatct tccccagtat atagctgatc
    11101 caatccaaat attcaatatc cgttcaggag gcttagctat ttatggcgga ctgattgcag
    11161 gcgcgatcac gctgtatttt tttactaaat atcattttat ttctttgtgg acttttttag
    11221 atatcgctgc accaagtgta ttattagcac aggcaatcgg tcgttgggga aatttcatga
    11281 accacgaagc atatggacca gacacgacgc gaagcttctt agaaagtctg catttaccgc
    11341 aattcatcat tgataatatg tatatcgaag ggatctatcg tcaaccaaca tttctttatg
    11401 aatcactttg gagtttgatt ggatttattt tgcttttgtt tttacgaaag aaaccacacc
    11461 ttttgaaaaa aggcgaagtc cttttatgtt atctaatgtg gtatagtttc gggcgtttct
    11521 ttattgaagg attacgtaca gacagtcttt actttttagg tgtgatccgt atttctcaat
    11581 tattatctgc tgtgttattt gtcggtacat tgattctatt tatttggcgc agaaaaaaag
    11641 gaaatttacc ggattatgac cgctcattag gaaaaaatca acaaattatc tgatttttag
    11701 agcgttaaaa atgaaaaaaa tttcaggagt ggtaaaatga attatagagt agcggtttta
    11761 ggaccaggct cttggggtac tgctttggca caagtcctcg ccgaaaacgg acatgacgtt
    11821 cggatttggg gacatcgtgc ttcacaagta gaagaaatca acacccaaca cacgaatcaa
    11881 cgttatttac cagatcatta tctacccgat tccatccaag cattcaccaa tatggaagat
    11941 gcagtaaaag acgcagatgc ggtgctattc gtcattccaa cgaaagcaat acgttctgtt
    12001 gcaaaagaac tagtgcctaa attgaataca aagccagtga tcatccatgc aagtaaagga
    12061 ttagaacagg gaacacataa aaggatctct gacatcttgt tagaggaaat cccagcagaa
    12121 aaaagaaaag ctgttgttgt tctttctgga ccaagccatg cagaagaagt agcgaaacat
    12181 gacatcacta cgatcacagc tgcaagtaca gatgcaaaag cagcaaaata tgtacaagag
    12241 ctgttcatga ataattattt tcggatctat accaatcctg atgtgatcgg tgtagagatg
    12301 ggggcagctt tgaaaaatat tattgctatt ggtgctggcg ctattcacgg cttagggttt
    12361 ggcgataatg caaaggcagc aatcatgacg cgcggattag ctgagatcag ccgtttaggt
    12421 gtagcaatgg gtgccaaccc gctgaccttt atcggattaa gcggagtagg cgatctagtc
    12481 gttacttgca caagtgttca ttctaggaac tggcgcgcag gcaacttgtt aggacaaggg
    12541 cagcccctag aagaggtgct agccaatatg ggaatggtcg ttgaaggtgt gaatactact
    12601 caagcagcga tggaactagc ccaaataatg gaaatcgaaa tgcccatcac tcaaacaatc
    12661 tatgaagtgc tgtatgaaca aaaagaaatc aaaacagccg caaaagaaat catgttacgt
    12721 gatgggaaaa aagaaaatga attctcatta acaatttttt gaaggagcga atgtatatga
    12781 aagtcaaaaa agcagttatt ccagcggcag gattaggaac aagattttta ccagcaacaa
    12841 aagctatggc aaaggaaatg ttgccaattg ttgataaacc aacaatccag ttcatcgttg
    12901 aagaagcgtt gaaatcagga atcgaagaca tcttgatcgt tacaggaaaa gctaaacgac
    12961 caatcgaaga tcactttgat tcaaatattg aattagaaac aaacctaagt gaaaaaggaa
    13021 agaccgaatt attgaaatta gtagaagaaa cgacagatgt caacttgcat ttcatccgtc
    13081 aatctcatcc aaagggttta ggacatgccg ttcttcaagc aagagccttt attggaaatg
    13141 aaccgtttgt cgttatgcta ggcgatgata tcatggaaga tgaagtacca ttgacacagc
    13201 aattaatcga tgattacgaa cgtactcatg cttctacgat cgccgttatg caagttcctc
    13261 atgatgaaac ttcaaaatac ggaattatca atccaggaga agtattggaa gatgggttat
    13321 acaatgtcaa aaactttgtt gaaaaaccag atccaagcaa agcaccaagt gatttagcta
    13381 ttattggacg ttacttattg actccagaaa tttttgatgt attgtccgag caacaaccag
    13441 gagccggaaa tgaaatccaa ctaactgacg caatcgatac attgaataaa acacaacgcg
    13501 tatttgcccg taaattcaca ggtaaacgtt acgatgtcgg tgacaaattt ggttttatga
    13561 aaacaagtat tgaatatggt ctaacacatc cagaagtagg tgcaccttta cgtgagtata
    13621 tcctgtcttt aggtaaaaaa ttagaagcag aaggaagcaa gatcgatcat aatccaaaag
    13681 gaataacaga aatcaaagaa gaaaagaaaa aataaccctt ttttctaacg agcttcagct
    13741 gctaattgaa taaaaagtag ttgaagctcg tttttatttg tttaaaaaac acgaaaatgc
    13801 tatccttata gaaaagagag acaagaaggg agtttgatca tgtcaggagg agaaattgca
    13861 ggattgatcg cagcaatcgc ctttgcgata cttgtcgttt ttatcgttcg tgtgttgttg
    13921 caagtttcta aaactttaga aaaagtagat cagactgtag cagaagcaaa tacgacgatc
    13981 gaaatcgtca caaaagacgt agatcttttg tctagacaag tagaaggctt gctggtcaaa
    14041 agtaatgaac tgctcttgga tatcaatgga aaagtcgcaa cgatcgatcc attgttcaca
    14101 gcagttgctg atttaagtga aagtgtatca gaacttaatc attctagtaa aaacatcgcg
    14161 acgaaagtcg gcggaatcgg taaaacgaca gctaaagcga cggttgccgg aaaagtcggt
    14221 gggacagcga tgaaattttt taaaaacaaa aaacatactg aaacgacagg aggaaaataa
    14281 aaatggctaa aaaaggtgga tttttattag gtgcagtagt cggaggaaca gccgcagcaa
    14341 tcgccgcgtt attgttagca ccaaaatctg gtaaagaact tcgggatgaa ttagctgccc
    14401 aagcggatga tttgatggat atggcttcag attacacaga tatggcgaaa gaaaaatcga
    14461 ctgagttaac aggtattgca aaagaaaaag cgcaagatct ttctcaacaa gcaggtgatc
    14521 tagcaggtag tgtcaaacaa aaagtaagtg aatcagcaga tgaagcagga agtgaatctg
    14581 acgatatctt gttaaatctt aaagaacaat cagctgactt atctaatcgt ttcaaaaaaa
    14641 cggcagaaga tatgaaaaaa cctgcagatg aattaggaag tattgctaaa gatacttcag
    14701 aagatatcat gattgatgta aaagacgctg ctggtaaagc aggagatact gtcaatgaag
    14761 gaatgtcaga agcaaaacca gttgcagatg acacaaaaga aatgatgaaa atggcaaaag
    14821 aagatgcgaa agaagctaaa aaagaaataa aagatacaat gacagattca tctacaaatc
    14881 actaaaatca ctaaaatcac aaatttatag aataaaaagg tgggagcgga ctagctctca
    14941 ccttttttac tcttattttt ctaaaactag taattctttt ggtgtagtcg tgaatggcac
    15001 acagccatcg cttgtcacat atacacagtc ttcgatacgt acccctactt tttcaggaat
    15061 atagattcct ggttcaagag agaagcacat cccttcttcg attactaagt catttccttc
    15121 tacaagggaa gggaattcgt ggacggttgt tccgatcccg tgacctaaac ggtgattgaa
    15181 atattcgccg tagccatagc tgttgatcac gtttcgtgcg atttgatcaa gttcacctgc
    15241 tgtcacacct ggacggacgg cttcttgtgc ggcaagctgt gcttcaagag tgatgttgta
    15301 tattttttct tggaagtcgg ttggtttttg ataagcaatt gtacgagtag catcactgca
    15361 atagccattc caaacaacgc caagatcgaa taacacaagt tcattcggtg aaatcttcgt
    15421 gtttcctggt gtaccatgag ggcttgcagc atttgctcct gcaaggacta atgtgtcgaa
    15481 agacatttgt gaaacacctt gttttttcaa ttggtattcg atttctgcaa tgatcgcttg
    15541 ttcagtcacg ccttctttca ctgctttaaa gccaatctca aatgcaacat cggcccattt
    15601 accagcttcg atcagtttgt ctttttcctc tagagttttg atcagttgta gacgttggat
    15661 gatggaagct agatcagtgt aagaatccgt tcccgtcaag atatcagaga tagcatcgaa
    15721 gcgctcgaca gtcaaggcgt tcttttcaat ccctacattg ccaggctgac catagcgagc
    15781 acgaagttct tttccgatga tctcccaagg gttttcgctg tctaaatagc cacggacatc
    15841 atacgcccaa ctactgtttt gagcatcttc aacttccaaa gcaggagtga aaaggaaagg
    15901 atcttgttca agagggataa aaagagctag tacacgctca tgaggatcac tttcgtatcc
    15961 ggaaaagtaa gcgatgtggc cagggtcgct gatataagcc aattcgactt ggtgttgtgc
    16021 catccattga cgtagttcat tgatttttac gttattcatt tggcaaccta ctttctaaat
    16081 taattttgta ttcaataatt gtaccactga tttattaaaa gaccaacgtt ctaaaaactg
    16141 aaaaaagtaa aaattttcat gaaaacagag ataaaacggt tgcaattctt gagattattt
    16201 gatattctat tttagaaatg taagacgtat tttcagaaaa catataaaat atgaatagga
    16261 gaacacaaat ggaaaagcaa acaatcacta tctatgatgt agcgagagag gcaaatgttt
    16321 cgatggctac tgtttcacgt gtcgtaaatg gcaatccaaa cgtcaaacca gcaacaagaa
    16381 aaaaagtctt ggaagttatc gatcgcttgg attatcgtcc aaacgcagtt gcgcgtggct
    16441 tggcaagtaa gaaaacaaca acagttgggg taatcatccc tgatgtcagc aatatgttct
    16501 ttgcttcttt agcacgtggt atcgatgatg tggcaacaat gtacaaatac aatattatct
    16561 tagcaaattc agatggtaac gaccaaaaag aagtcaatgt attgaataac ttactggcta
    16621 agcaagtgga cggcgtgatc ttcatgggtc accatatcac agacgaaatc cgtggagaat
    16681 tttcacgttc aaaaactcct gttgtattag ctggctctat tgatccagat gaacaagtag
    16741 gtagcgtaaa cattgattat gtatcagcaa caaaagatgc agtaaacaaa ttggcaaaaa
    16801 gcggcaataa aaaaatcgct tttgtcagtg gtgcgttgat cgatcctatc aatggacaaa
    16861 atcgtctgaa aggctataaa gaagcattaa gcgaaaataa cctaccatat agcgaaggat
    16921 tgatctttga agcacaatac aacttcaaag atggtctatc tctagtcgat cggatccata
    16981 atagcggtgc aacagctgtc tatgtttcag atgatgaatt agccatcggt attttggatg
    17041 gtttattaga ccgtggcgtg aaagtaccgg aagaattcga aatcatcaca agtaacaatt
    17101 cactactgac agaagtagct cgtcctcgtt taacaagtat cacacaacca ttatacgatt
    17161 taggggcggt atctatgcgc ctattgacga aaatgatgaa taaagaagaa gtcgaagaaa
    17221 aaacgattat cttgccttat ggaatcgaag aaaaaggatc tacaaaataa taaaaggaca
    17281 accattcaga ggttgtgagc ctgccgttta actctgcgtt acaaggagca atttcaaaaa
    17341 acagcatttc atgtttgatg aatattgtga ctcgtgattc ggagttggca ggagaacacc
    17401 gtttattcgg gaaaaggggt tatgacaaaa ttttgtttaa tccttttttt ttgtcttaaa
    17461 aaagagagaa agtggttgaa attacattta tcgtatataa aacaatccgt gtatagatgc
    17521 tgcattatgt tcaccaccat caaaaaaata agggactgtg tattctatta aatcaatcat
    17581 ctttctcatc tgattctttg gttgcttgat tcgctcttgc ataagctgaa gcggtgttcc
    17641 agtaatctgt atagttttca tcggtaccgc caacgccaaa tttaaaggcg cttttcttcg
    17701 gtccattttt cgcccatagc gattcaactg tttttcctgg tgtttggatg gattttttat
    17761 tgacagtaac ttttccaggt gtttgaccag taaatccaga tacttttttc ttcatcacat
    17821 cagatgataa ttcgaatttc tcatcagtgc caaaaatgtc aggttttgct tggtaagctt
    17881 gattgatcag attggcaaga taattagccg aacgtatcct tgctttgctg tccattccag
    17941 tattgtcatc gtgccctgcc cagctactga tggtaataga cggagtagag acaattagcc
    18001 atgaatctcg gtattcattg gtagaaccag tttttccgac ccaatctgct tttcctagat
    18061 ttccgtttaa gcttgagatg gcatctttga atggtgtagt gatttttgca ttgatcacac
    18121 tgcgcatcat gtcattcatg atcgatgccg ttgcttccga atagatccgg acagagttgc
    18181 tttcgtgttt ataaattaca tttcctgcgt tatctgtgat ggaatcgatg atatagcctt
    18241 cttcatagac gccgccgttt gctaaagcct gaaatccatt ggtctgttga agcgtcgtga
    18301 catctactgt acctaaagga gcagattcaa cgccccaatt gtcatttgcc gggtagttca
    18361 tctttgccaa gtagtgctcg taagcatatt gtttcgatcc accggaattc aacacatctt
    18421 gatataagtg ataagccggg atattgttgg accattctag tgactcacgg acggtttgga
    18481 aggtgttcga tccttcgtta gtagcattga cgattttctc gccagcattt tctccttctt
    18541 gccaagttgt tgcataatct gatacacggg attctgatcc gataagcccc atgtcgattg
    18601 caggtccata aacaagaact ggtttgattg atgagccagc ttgacgttct gtatcgaagg
    18661 catggttatt ttgattctga ctgtaattcc gtccgccaac gaatccgtag atgcggccag
    18721 ttctgttatc catcaataca tttccggttt ctacttgact gccagcacca tcatctaaca
    18781 tgtatccata attttctacg ccattttgca atgcagcata gacatcttta tcgattgtcg
    18841 aatggattgt atatccttta ttttggatcg tttgctgtgc cttttgataa taagcgtcac
    18901 tgaccgatga atcagacata tcagcgttgt ctttttcagc caattgttgt gcaatgattc
    18961 ttgttgcttc attcatcaca gtataataca agaattctct gtcagtctgt tctgcgattt
    19021 gttgtggtag aaagtctttt gtaaggtcat aggcttttgc ttcttcgtat tgttctttcg
    19081 taatttggcc ttcacgatac atactgaata aaacaaaatc ttttctggca aggccggcag
    19141 aaagatcttc ttttagcgca cctgtgttcg tgtatggact atacgtgatc gggctttggg
    19201 gtaatccagc gatataagca gcttgcggca gcgtcacatc ttttgcatct acaccaaaga
    19261 ttcctcttgc agcttcctgg actccagcaa tgttttgtcc cttattatta cgaccaaatg
    19321 gtgagacatt caaatacgta gcaatgatct catcttttga aaagtatttt tctacttgtg
    19381 cagaaagaat gatctcgttt gctttacgtt tgaatgttgt ttcatctgta aggatctgct
    19441 gtttgactaa ttgctgtgtc aaagttgatc caccagaaga gccaatacca gtagcttcag
    19501 atactaatgc tcgtaaaaca gctttaggaa cgtaaccttg gtgtttatcg aagtattcat
    19561 cctctgtgct gataattgca gttttcaata aaggtgaaat tttatcagat gaaatagtcg
    19621 tgcgcatcag atctgtctgg atttttgata tctctgtatt atctgcataa acaatctttg
    19681 acgtttctgt aatgtttcct agttctgtct gcagtgcttt tttagtcggc aattgtgtgt
    19741 cttcgacgag ataggcaaaa tagccagcgc ctattccaat acctagagct gcgaacatca
    19801 aaagaagaac agtaataaaa acagtcaatg attgaaaaac tctaaaaata atcttaggaa
    19861 caaaccattt ttcttttttt cttctattct gtttttttcg agtatttttg cgtggcaaaa
    19921 gggtcacctc aactaatggt tttatttcgc taattatagc aaataataga acaaaaaaac
    19981 agacaaggct gtttttggca ggaatttctt ttttaaaaga gttaaaacgt gagaaaatga
    20041 tcttttttat aaaagagttc ttaagctttt ctttagagaa aatttcagtt actatggaga
    20101 acagggcaaa gaaaaatcaa tcattttctt tgtatatcat cttaccaata tataggaatt
    20161 gttagtaaca attgcctttc aaggaggaat ccatccatgt tattaggctc acacgtcagc
    20221 atgagcggaa agaaaatgct gttaggagca gcagaagaag cagccagtta tggatcttcg
    20281 acttttatga tctatacagg cgcccctcaa aacactagaa gaaaagccat cgaagagatg
    20341 aatattgatg ccggaaaagc gtacatggca gaaaacggtc taacaaatat cgtcgttcat
    20401 gccccttata tcattaattt ggggaatacg atcaaaccag aaatgtttcc ttttgccgtc
    20461 caatttctga gagaagagat tgttcgtgcg gaagctttag gcgctactca aattacactt
    20521 cacccaggtg ctcatgtagg ggcaggaaca gaagccggaa ttgctagaat tattgaaggc
    20581 ttgaatgaag ttttgacaaa agaacagacg gcacaaatcg ctttggagac aatggcaggt
    20641 aaaggtacgg aaatcggtcg aacatttgag gaattagcgc aaatcatcga tggagtaaaa
    20701 ttgaatgaaa aattatccgt tacttttgac acctgtcata cgaatgatgc cggttataat
    20761 attaaagaag acttttcagg tgtaatggaa gagtttgata agatcatcgg gttagatcgg
    20821 cttaaagtac tccatatcaa tgattctaaa aatccgcaag gttcgcataa agaccggcac
    20881 gccaatattg gttttggaac aatcggattc gacacgctga ataagatcgt ccatgatcca
    20941 atgtttgaca atatttcgaa aatcttggaa actccctatg tcggcaaaga taaaaagaaa
    21001 gcttacgcac catatggaaa agaaatcgcc atgttcaaag cacaaatatt cgatccagaa
    21061 gtgtttcctg agttattagc agaagaagtg atactctaag attttctatc tgacagcagc
    21121 aaaaaaggac caggcacatt gccttgtcct tttttgttta agaaacaaag gagtgctttt
    21181 tgcagtcttt ttcatcatgc atcttctccc ccctcttttt aaatttagac aaacagggta
    21241 tactggtaga aaagtaaaaa aacgaaaagt ttcacaagca agtggcaaaa cgtgattgga
    21301 aggacgaaaa gatgctgaac actttaaaac ccttgatcaa ccaactaaaa aagaaacata
    21361 catactttat tcttggcata gccctattat ttacaattat tcgtttgtat ttatatagta
    21421 aagcaatcta tggaacagac atgtcggcaa cctatgatga ccagctgctg attcgctatg
    21481 ctcaaaatat tttggacggt aaatggctag gggagtatac gactacaaca ctttccaaag
    21541 gaatctccta cagcttattt ttagtacttg cacataaact gcatctttct tatgcagttt
    21601 tgttcggcat gttcagtatt tttgccagta tattggttgc actcgcgttt aaaccgatcg
    21661 tgaagaacaa gtatcttctt ttaagtttct atttttattt tttgttttct ccgattagtt
    21721 ttacacatga atattccact cgaacgtatc gtaatgccct cgtgattccg gctgttgaga
    21781 tcattatcgc ttgtttacta gctatttatt accgtaaata tagttccagc aaaaaaatgc
    21841 tgccgtggtt tttagggcta gctgtttctt ttcctttctt ttggttcatt cgcgaagatt
    21901 ccatctggct tcttcctttt actgtagtcg cgttaggcat cagtttcgtc cagatcttgc
    21961 taggacaaga cttttctttc aaagaagcaa agaagttaaa agaacgacca tttctccttt
    22021 cgaaacaaga atggctgaaa ctttcattgt ttttactccc tctagtcggt ttctccatcg
    22081 tctatcagtg gatcgaatat aaaaacgaga caacatatag tattgccgcc atcaatgacc
    22141 gttcgagtgg cgaatttgga cgtttgatga aacagctgat ccgaatagat gacgaaacga
    22201 acttaaataa agaaaacagt gagatatggg tctcgcatgc tgcattaaat aaagcattag
    22261 acgcatctcc tacatttcat gctgtttcta ctcgtattga cgaattgtat acgactcatg
    22321 cgtggaccca aggtggaaaa gtaaaagaat taccaggaga tatcattttt tgggcattgc
    22381 gagatgtggt aagcgaatct ggttattacc aaaacaatgc aaaagaaacc aatgattttt
    22441 ggaaaaaagt caatcaagaa ttagaagaag cctatcaaaa gggaactatc aaaaagaaaa
    22501 aagaattcta tctgatggga tcaggggatg gaaagacgat gaatgatctg ccaatcgtat
    22561 ttgcttttct gaaatcagga tatgcctatg catggcgata caaagatttc catcaagggg
    22621 ggaattatag tttcggtacg caagaacaag tcgaacaggc ccaagaatta ttgaatcttc
    22681 cactaatgga taattggatc gatagcagtg atccgagaac acctgatttc cagttgacaa
    22741 aaacagcaaa agtcgcgaat gtcatgataa aaatttacca agttctggcc attcccgtgc
    22801 tgattgcttc aatagcaggg atattactta tgatactagg aaccgcattt gcaaaagctg
    22861 atcgtgccta ttatagttcc tctttgattg tagtcaccgg attaatactg acacaattgg
    22921 tttttctttt cggcgtctcg tggttttgtt cttatgcgcc agaaaaaaag gattattttt
    22981 taagtgtcta cactggcgca ggcgtgccat taacgcaatt catcgtagta acaagcatgc
    23041 tagttttcag taaattgaat ctgaaaaaag ctttatcttc caaataaaaa catactaaga
    23101 ttagaggaga acatcattga cgaatgatgt tctcctctat tttctatttt tagattatag
    23161 tgaagagtga aagaaagttc gaacaaagcc ttgaggacta tgagtcccta tcaaaaaccg
    23221 aacttcttta ctgtcatttt agaatcaggt ttacgtatgt tggggccatg agcccgtgta
    23281 taggaaattg gaatcgaaat gaagcagtaa agttctttca aaacttaaaa aggaggaaaa
    23341 caaatgcctt acattcttgc attagatgtt tcaatgggac acagctacag tgtcctatac
    23401 aaagacgaca cctgcttatt tgaagacgag atcgaacaca ctcaaagagg atttcatgca
    23461 ttactcgaag agatccagca cttgcctgaa cgtccattga ttgtttttga agcaacgggg
    23521 atttattcca gaccagtcga aaagttcttt caagatcatc acttttctta ctgtttgtta
    23581 aacccgttag aagcgaaaaa acagatggaa gaaaacacct tatgcagttg gaaaacagat
    23641 aaatcggatg cccatcggct cgcacagact catcttaaaa atcaacgtca gccaaaagaa
    23701 gtacaaagca atttttactt agagatgcgc gatttagccc gtttttatca agaaattgag
    23761 aagaaaatca ctcgtctgcg catggattta cacaactgcc ttcagttgac ttttccagag
    23821 cttgaacagt tctttttaaa caggctgacc ccttacgcat tgatattgat ccgtcttttt
    23881 ccccatccag attttgtatt ggcatctact cggacaaaaa taaaaaataa actgatcaac
    23941 gaaacacgga aaaaaatctc cgcgaatcgt gccgagcaaa aggcagatca aatcatccac
    24001 tatgcacagt gtgcctatcc agctgttgaa aaagacagta tccactgtca aaaaacgatc
    24061 tattacgctg aacttctcca agatttactc gaacaaaaag aagcactggc gacacaaatg
    24121 atcaaaaaag ccgaaggctc tccttgcttt ttgttgtacc aaacgttccc tgggattggc
    24181 gcactcacag ccgctttatt gctcggtgaa ttaggagata tcacacgatt taaaacccat
    24241 aaacaattga atgcctttgt cggaatcgac atccgacggt atcattcagg taagtatact
    24301 ggccaagacc gcatcaataa gcgtggtaat cccaaagcac ggaaaattat tttcttcacc
    24361 attcgaaata tgattcgcca gcaacgcgca gctcctaatc atattgtcga ttattactac
    24421 aaattaaaaa agcaacctat ccctaagaaa gataaggttg ccacagttgc ttgtatgaac
    24481 aagcttctga aatgtatgca cgctatggtt agggcacata cagagtacga ctatgcgtac
    24541 gcggtctcag tggaccatta actaaaccac ttacagtata ccacttcttt gcccagatcg
    24601 aaaatttttt acgaacgggg ctttatttgg tgtgccttga ttttgtcgct ttcgtttatg
    24661 agctattttt cttacacctt ttacttgact aatcgtagga aagggaactg tgacaaaagt
    24721 cttgtcgcag ttcctttttc cgaataaacg gtgctcaaaa gcttgcatct gcggtaataa
    24781 gtccaaccaa gttaaaaaca taaaaagctg tttttacttg gctgttctta ttacttgatg
    24841 caaggcagct ttttcacaac ctctgataca cggtgttcca gaagtaactt cttcggaaat
    24901 aagccgaaat ttcacaaaaa tttgaaaagc aattttctta aattccttct tatttcttga
    24961 agctgcccac ttctgacaca acctcctttg tgtaaaagaa tcagtttcta ctcgatttct
    25021 tctatcgttt cctgcaaacg ctgttcatat tttttgtttc ctttatataa atcaccaatc
    25081 gtccatgcca acaggagaag gacaagaaca atgattacat aagcgatttg atcagccaat
    25141 gctagttgag ctgaggtggc ttgctcgccg aagaaacttt cgatttgacc tggtaatcct
    25201 cgtaaattca agtaagtcag actaatcaca gagaaccagc caagtagttt tacaacaaat
    25261 gaatttttga atcggctccc catctccgct tggctattgg tcatcatcag taaaggaatc
    25321 atcgagaatg gcaatgcaaa agccagaaaa acttgggatt cattcatcaa gttattcaaa
    25381 gcggtatgtt cttcgatcgt tccttttgta cttgtaaaca aaacacaaat caatacagga
    25441 atcacagaaa gcaatcgtgt gactagtctt cttagccaaa ttgggatacg catatggatg
    25501 aagccttcca tgatgacttg accggtcaac gtaccagtaa tagtagagtt ctggccagaa
    25561 gcaagaagtg ctacagcaaa taaagtagac aagataccag attttgctac agcaatcaag
    25621 ataccattgc tcatagctga tggatcagat aaagcttcat acagaccaaa aaaggaagga
    25681 tcttggacag aaccggtttt gaatacagcc acacccatga tcaatagcag tgcattgact
    25741 agaaaagcca ttgacaactg gatattagaa tcccaagtag aaaagcgtac cgcttgtgct
    25801 acatcttctt cgtcttggtg atcgatcttc cgagtttgag aaactgctga atgaagatat
    25861 aagttatgcg gcatgactgt tgcaccaata atccctaaag ctccagtcag cggagtttcg
    25921 ccgccgatga ccggtgagtc tgagaatgtg tgactgttag ggataaatcc ggcaaacaaa
    25981 ctgtgccaat cagggttaga caagccaact tgatagacga aaacgaataa aatgacgaaa
    26041 atcaagcaga caaccaaagc ttcgatttta cgaaacccga cttttgtcag caataaaagg
    26101 acgaaaacat ctaacacagt gataaatacg gcaattacca atggaatatc aaaaagcagg
    26161 tagagggcga ttgctgcacc aatgacttct gcgatgtctg ttgccataat ggctaattca
    26221 gtcaagatcc ataaaatgac acccaatgtc ttacttgtcc gtgctcggat tgcttgggcc
    26281 aaatccattt gggtcacgat tcctaatttt gctgccatat attgtaaaag catagcaatc
    26341 agactagaca tcaaaataac tgacatcaat aaatattgga aattttgtcc accagtgatc
    26401 gaagtggacc agttacctgg atccatataa ccaactgcta ctaatgcacc tggtccagag
    26461 taagcaagaa gtgttttaaa aaaacttttt cccttgggta catccactgt tccatttact
    26521 tcagctaaag ataaaccatt tgtatgttca ataagtcgat gcttatgaaa ttgtttgttg
    26581 gattgattat tcaagaaaaa accacctttc ctatttatct acgattacat tctacacctc
    26641 agaaagaaaa aattaaagaa aaagatttgg tatgcctaat tttttttcgt ggcgtgtctt
    26701 gctggttaat gaaagaaatt ttgttcttct ctaaaaaaac cactattttt tgcatgaaac
    26761 taaagaatac gcctttcttt tttcaataaa ctttagtaaa ataaatcatt gagagcaatt
    26821 cggaaataga aaggagagat ttatatggta tcaacaggta ttgtcgtact tattgctatt
    26881 attgctttgc taatcggcgc agtaggtggc tttttcctag cgcgtaaata tatgaaagac
    26941 tatttagaaa aaaatcctcc cgttaatgag gacatgttgc gatcaatgat gatgtcaatg
    27001 gggcaaaaac catctgagaa gaaaattcgc cagatgatgc agcaaatgaa gaaccaaggg
    27061 aaaaaatagg ttctgaccca aagacttttt gcgggctgat tttttcagtc cgttttttat
    27121 ttcgtaattt gttagactat tcagactatt cagcaaaaaa gacaagcaca gacaatccta
    27181 tgctcttgat aaagacaaag gaggagtaaa atgtcgattt ttagaaaatt aggttggttt
    27241 tttcgacagg aaaagaaaca ttatttaatt gggattttct ccctattcgc agtggccttg
    27301 gtacagttag ctcctccaaa agtgattggg atcatcattg atgagatcgc cgataaaaat
    27361 ttatcaataa agatcatttc ctggtggatc ttattattga tccttgcagc tctcttacaa
    27421 tatctttttc gttatatctg gcggacgaat atttggggaa gtgcagctcg gttggagaaa
    27481 gaacttcggc agcagctatt caaacatttt acagaaatgg acaatctctt ttaccaaaaa
    27541 tatcggactg gagatttgat ggctcatgca acgaatgatt tgaatgcaat ccaaaatgta
    27601 gcaggagctg gtatcttgac ctttgcagat tcagtgatca cgggcggaac aacgatcatt
    27661 gcgatgattt tgtttattga ttggcgctta acactgattg cgctgattcc tctgccttta
    27721 ctagccgtta cttcacgagt gctggggtca aaactccacg atgcttttcg tgattcgcaa
    27781 gaagcttttt ctagtatcaa tgataagaca caagaaagta ttactgggat caaagtcatt
    27841 aaaacatttg gacaagaaaa agaagatctc gcggattttg aagaaaagat tgaagatgca
    27901 attattaaaa acaaacgagt caatttttta gactccttat ttgatccatt cattaccttg
    27961 attatcggtc tgtcttatgt tgcaacaatt attttaggtg gaaagtatgt cttggaaggc
    28021 acgatctcaa tcgggcagat ggtctcattt attagctata tcggtatgct tgtttggccg
    28081 atgtttgcga ttggtcgatt gttcaacgta ttggaaagag gaaatgcttc ttatgatcgc
    28141 gtcaatgaac ttttgcatga aaagacgcat atcgtggaac aactgggtgc agtgcagact
    28201 ccggcaaaag gcgagctgaa tgtagctatt tcctctttta actatccgaa agaagaatca
    28261 ccagcattag aacaaatcaa atttgcgatc caagaaggag aaacattagg tattgttggg
    28321 aaaactggtg cgggaaaaac gacaatcatc aaattactga tgcgtgaata cgatgattat
    28381 caaggaaaaa ttcaatttgg cggatgttcg atcaaagaat attcattgaa tgctttatta
    28441 ggatcaatcg gctatgtgcc tcaagatcac ttcttgtttt cgatgaccat tcgagataat
    28501 atccgttttt ctaatccaaa actttcagaa gaagaagtag agcatgcggc ttctttagca
    28561 tttatcaaca atgaaatcaa ggaattacca aaaggttatg ataccttagt tggagaacgt
    28621 ggagtctctt tatctggcgg acagaaacaa aggatttcga ttgctcgagc attgatcaaa
    28681 aatcctgaat tgctgatctt agatgatgca ctttctgcag tagatgccaa aacagaagaa
    28741 gctatcttgc ataatttaaa agaaataaga aaagagaaaa caaccatcat tgcagctcac
    28801 cgtttaagca gcgtgatgca tgcaaaagaa atcattgttg tcgatcaagg gaaaatcatt
    28861 gaacgaggaa cccatcatga gctgcttgca ttaggcggct ggtatgaacg aatgtgggag
    28921 aaacagaaat tagaagcgaa gatagaagga ggcgaggcct gatggatgaa aattatcaat
    28981 cggaatggac aaaatcgatt ccacttaaag aacagaagaa gatcatcaaa cgactgatga
    29041 agttcgccaa accttttaga agaacgttca tcgttgctat cttatttgcg tttgccctgt
    29101 ctgtgatcaa tgtcctattg ccaaggatca tccaaacttt tatggatgat catctggcta
    29161 aacagtcggc tacgacacaa gttattttgt tttttgctgg tatgtacttg tttggagtca
    29221 tagtcaaaag tatcatttgg tttttccaat ggtatttgta ttccatggct tcattgaaga
    29281 cctatcagta tgttcgggta agattatttg aaaagctgca tacactaggt atgcgttatt
    29341 ttgatcagac accggcagga tcgactgttt ctcgagtgac aaatgacaca gaaacgctat
    29401 ttgaattttg gtatgtcttt ttgatggtat tgactgggat ctttgcagtc gtttcttcct
    29461 tcattgccat gtttcaaatc aatggagaaa ttgccttgtg gactttggtg ttcttgccga
    29521 ttttaggaat cgtcatctgg tattaccaga aattcagttc tcgtatttac cggaatatgc
    29581 gggaaaaatt aagtcagctg aacacgaaat tgaatgaatc gatttccggc atgcgtatta
    29641 tccagcagtt tcgccaagaa gctcgtttag ctaaagaatt tgaacaagtg aacaacgagt
    29701 atttagacac gcgctatgcg atgatcaaaa caaattcgat tctgttaagt ccgatcatca
    29761 attttctata tgcattggcg attgcgttgg cgttgacttt gtttgggatc gatgcgctgc
    29821 attctcccgt agaagttgga ctgatctatg catttacgac ctatgtccag ggatttttta
    29881 atccaatgtc tcaaatgatg gactttttga gcacctttac agatggaatg gttgccggta
    29941 gtcgtatttt gaaaatcatg gatacagaag aatatgcacc gcaacaaaat cctggagcag
    30001 atcaagcgat tacttcaggt aagatcgagt ttcgcaatgt aagcttttct tatgatggag
    30061 agcacaatgt attgaacaat atctcttttg tcgctaatcc gggagagacc gttgcattag
    30121 tggggcacac cggaagcgga aaaagctcga tcatcaacgt attgatgcgg ttttacgaat
    30181 tttatgaagg ccagatcttg attgatgatc atgacattcg agaatatcca atggaagagt
    30241 tacgtaagaa aatgggattg gtcttgcaag atgccttctt gttttatgga gatatcgcag
    30301 gaaatatccg gatgatgaat ccaactatca cagacgaaca gattcgccat gcagctgagt
    30361 ttgttcaagc aaacaagttc atcgatgaat taccaaaagg atatcactca aaagtgatcg
    30421 aacacggtgc cagctattct agcggacaac gacagctgat cacttttgcg cgaacaatcg
    30481 tgacagatcc taaaatacta gtattggatg aagcgacagc aaatatcgat actgaaacag
    30541 aaggattgat ccaagaagga ttgacaaaaa tgcgacaagg acggacaacg attgcgattg
    30601 cccatcggct gtccacgatt cgggatgcag aattgatttt agttttagac aaaggtcata
    30661 tagtagaacg tgggaatcat gaaacacttc tagcgcaaaa tggtttgtat gcagatatgt
    30721 acaaactgca aagcagtggt gtgtaaaaag tggttgtgac agggctccgt tcagctttag
    30781 gtgataagaa aaaaattgta aaaaaggctt tccagatttt tgcataaatt tggcttatca
    30841 ccgataagct ggtactggaa caccgtatat tagaagttgg gacagtagca tagtagcatt
    30901 ttgacctaag cgctagagtg aaaataaaac agagcttccc agattcttca tctgtgagcg
    30961 ttagtgtcgt aattaccaga tttaaaaaaa atcacgacag ctagtcagaa gtgcccaata
    31021 gccgctaaga aagcgttgac aaaagtgctt gctttcgata atataaagaa aacgatacaa
    31081 cttggatagt gattgcctga tgcaagctaa acctgatgag tctcccatgg ttccatggac
    31141 ggctttttgg gtttttcttt ttgttttgga tacagcccta tatatcatgg aaaggagtaa
    31201 aacatgctga ttgagaaagt actgaataac aacgtcgtaa taaccaggga tgaaagaaat
    31261 caggaaatga tcgtgatggg aaaaggtttg gcattcaaaa agaaagtggg tgacccgatt
    31321 tttgactcat gcatcgacaa aaaatttcag ttgacaaatg acggactttc acaacagttc
    31381 caagaattgg cacaagcgat tcctcttgag tacttagaaa tttcttgtga aatcattgaa
    31441 tatacaaaag aggctctaca agtccagttg aatgattcgg tgtatattgc attgacggat
    31501 catttgtata cggctatcga acgtcacaaa atgggaatat ttgtaccaaa tgtattgctg
    31561 tatgatatca aacaactatt tcctaaagag tatgccatcg ggaaaaaaac agtgaagaaa
    31621 atctcggata aataccacat caagttatca gaagatgaag ccggatttat cgcattgcat
    31681 ttagtcaatg cacaaggcat tcaaagccaa caagacaata cctacctgtt tactacaaca
    31741 atacaggata ttctaaatat cgtccgctat tactataaga ttacatttga tgaagattca
    31801 gtccattttt ttcgattcgt cacacaccta cgtttttttc tgacacgcgt cctagatgga
    31861 gaaaaatcag aagtgggaga agtttctgag gaattgtacc aggttattca aaaaaaatat
    31921 aaggatacgt ttggttgcgt tgagaaaata gcagactttt tagccgagat attcaactac
    31981 cagatgtcca cggacgaaaa aatgtattta acgatccata ttgcgcgttt agtgcatgag
    32041 aatcgttgta aataaataaa aaaaggagga taaattaaca agaacgcatg aaataagggt
    32101 agtgacattc agttggctaa accttccact aataaattgg agggaataga aatgggaaaa
    32161 tatcaagaat tagcgagcaa aattgtagaa aatgtcggcg ggaaggaaaa tatcaatggt
    32221 ttgacacatt gtatcacaag gcttcgtttc aaattaaaaa acgaagaaaa agcaaatgat
    32281 gagatcttga aaaatatgga cggtgttgtg acggtaatgc gagcgggagg acagtaccaa
    32341 gttgtcatcg ggaatcatgt gccagtcgtg tttgaagagg ttataaaagc aggaaacctt
    32401 acttttgatg aggctgtttc aacagaaaaa atgagaccgt ttgatatgct tattgatgga
    32461 atatcgggct gtttccagcc attccttgct attctagctg ctggtgggat gattcgtgga
    32521 ctgacagcat tacttgtctt cttaggcgca tttgaccgcg gttctggcac atttgttatg
    32581 tttgataata tcggtgattc tgtattccaa tttatgcctg ttattatcgg tttaacagct
    32641 gcccgtaaat ttaaagtaaa tgaattcgta gggatgttgc ttggggcagc attaatgaat
    32701 cctagcttgt cgctggaagc tttgagtggt gcagcagaag caccactgac taccatattt
    32761 tctggcacta tttttgaagc acctatttat caaacagtat tcggcattcc ctggattgcg
    32821 cgtaactacg cttcttctgt cattcccatt atctttattg tgttgttagc tagccaaatt
    32881 cagaaaccaa ttaaaaagtt agtacctgag atgatcgcga atttcttcgt tccattcttc
    32941 accgttctta ttacaatgcc cttaggtttt ttactcgttg gaccagcatt cacattcgca
    33001 actgatattt taatggctgg atttgaaaca cttttagcac tttcgccagt tatttatgga
    33061 gcaatcgtag gcttcttctg gcaaattcta gtgatgtttg gtctccattg ggcaatcgta
    33121 ccaatgggat tgatgcaatt ttctgtgaat ggttggcaaa atatcatgac acctgttgca
    33181 gtagtgtctt ttggacaaac tgcagcactt actgcactct actttaagct tagaaaccca
    33241 aaagacaaag caatcgcaat accagccatt gtttctggta ttgtggggat tacagagcca
    33301 gctatttatg ggtttacatt gccacgtaaa aaaatattta tttttacctg tgtcggtggt
    33361 gcgttagggg gcgcgtatag tggattaatg aatttaactt cttggaatcc aggtggatta
    33421 ggcatcttta caattcccaa ctatattaga ccagatggtg atttaacgga tgtgattaat
    33481 gtattgattg gaatcgccat tgcgatggtg gtatcctttg cgctgacttt tttcttttgg
    33541 aaggaggaag caggtgaaac agatgataga cagaaaagat caggtaaaga aattgtaaaa
    33601 acaccgatac aaggacaaat cgctccactg aatgcagcaa aggatgcagc ttttgcacaa
    33661 ggaacactag gtcgaggaat cttgatctac cctgaaaaag gagaagtacg tgcccctttt
    33721 gacggaacag tcatgaccct gttcccgacg aaacatgcaa tcggaatggt ttctgaagca
    33781 ggtctggaat tgctgattca tgtcggattg gatactgttc agttggaagg gaaatatttt
    33841 gaaagcttgg tgcagcaagg agaaagagtg aaacaaggcg atctgttgct ccgcttcgac
    33901 aaaaagaaaa tcgaagagga aggatacagt ttagaaacac ctgtgatcgt caccaattac
    33961 acggattatt tggatatttt ggaaaatcaa ggggagacgg tcgggccaga cgatgcactt
    34021 atcaccgcac tgacctgaac ggttaggtga aagaatggac taggcttaat tattcttttt
    34081 agtccacttc tttattttca aaattgaaag gctctttcat gatggctgag ctgatagggc
    34141 gtttatcaaa tttttgtcct acagaaatta gatggactaa attctgttga taattttctg
    34201 caaaaacaca actatataac acgtctagaa gataacaaat cgatgtattg atcgtaaaat
    34261 taccaatttt cgaatacagt cgttcgcgtg tcgtaagtcg caaagcggct gtactcattg
    34321 aagatagcgt tgaatcaccg atactggtca aagctaaaat tggaacatga ttttgtttca
    34381 atatttctgc ggtacgaagg atcacattgt tttcacccgt ataagaaatc agaatcccgc
    34441 acatatcact cgtgcaattg tacgcttcat agaacgattc accaaaagtg gtgacaatcg
    34501 tgacatccat tttgatgcgc ttcattttca gcatgaaatc ttgagaaatc aaagtattcg
    34561 agttactggc gaaaatcacg attttttttg cttgaagcat tagttgtttg gctttttgca
    34621 gttggtcatg atgaatgaga gaaagtgtat cttgaatcgt catctgttct aaagaagcca
    34681 ttttatttgc gatcgtcata ataccgtctg tggtggaaaa aggaagattc gcatctacgt
    34741 cttgaaaatt cccttcaaga taggctgttt cagctaaaaa agcattacga aaactgaccc
    34801 agccatcaaa acccattttt tttgctacac ggatgagtgt ggacgggtgg acataacatc
    34861 gctcagcaat ctcttggatg gtcatttcgc caagtgctgg agaatcgctt aagagaaact
    34921 gaatcacttc attttctgaa ggagaaaagg agatttcttt gattttttca cgtaataaca
    34981 tgtagatacc ttcttcttgt ttttttacgt aaattataac ttttttgaaa atgatttgta
    35041 aacggattta tgttatatag cttaataaag acattttttg acaagatatt tacaaaaatc
    35101 cagtagaaaa cactaacttt tgtaaatgtt actggaaaga aacgaatttc tattgtttgt
    35161 tttgtttaca aaaaagataa taaaagtgta aaaagtaaag gagtgagaac atggcaaaag
    35221 gaatcaaaat cgctacgatt ggtggaggca gcagttacac ccccgagttg atggaaggat
    35281 ttatcaaaag atatgaggaa ttaccgatac gagaaatctg gctcgtagat gtagaagaag
    35341 gaaaagaaaa gcttgagatc gtcagtcaga tgtctcagcg tatgtgggat gcttctccgt
    35401 atgaagtcaa aatctatgca actctggatc gggaagaagc attgaaagat gcggattttg
    35461 ttacgactca gtttcgtgtc ggtcaattag atgcacgaat caaagacgag cggatcccta
    35521 gttattatgg gatgattggg caagaaacaa atggtgctgg cgggatattc aaagcttttc
    35581 gtaccgttcc agtggttttg gcaatcgttg aagacatgaa gcgtttatgt ccaaatgctt
    35641 ggctgatcaa tttcgcgaac ccaagcggga tggtgacaga agcagttatc agatatggca
    35701 aatgggataa agtgatagga ttatgcaatg tgccagtaat ggcgatgatg acagaaccaa
    35761 caatgcttgg caagaaaaaa gaagagctga tctacaagtt tgccggtcta aatcacttcc
    35821 attggcacaa agtagcagat aataaaggaa atgaccgcac ggcagaattg attgatcgtt
    35881 tatacgatga agacaatgga ttgccgaaaa atatctttga tatcccgttt ttcaaagaac
    35941 aattaaaaca aatgaacatg atcccttgtg gctaccaccg ctattactac cgtgaagaag
    36001 aaatgctgga acatgcttta gaagaatatc aaacgattgg tacaagagcg cagcaagtga
    36061 aagaaacaga agcagagctg tttgaattat acaaagatcc agaattggat cataaacctg
    36121 aacaattgca aaaacgaggt ggtgcttatt attcagatgc tgcctgtgaa acgattgctt
    36181 cgatctatgg aaataaagaa acacagatcg tggtatctac aaagaatgat ggagctgttc
    36241 ctgatctgcc ggcagattgt gtagtagaag tcactgccta tgttggcgca caaggcgcac
    36301 gaaatgtagc gtttggttcc cttcctgctg ctgaaagagg ctggttgcaa gtaatgaaga
    36361 atatggaatt gctgacgatc gaagcagctg tgactggtga ttatggaaca gctttacaag
    36421 cgtttacgat caatccgctt atacgatcag gaaagacagc aaaacggatc atgaatgagt
    36481 tgtttattgc acatcagcag tatttaccgc aattcaagga aacaatcagc cggttaaaag
    36541 cagaaggaat cgaagtccaa gatgaactgg caagagaatt atcaagcaat acacatgtaa
    36601 tggaggaagt atgatgggat tcagaaaaga ttttttatgg ggcggagcta cagcagccaa
    36661 tcaatgcgag ggcggttatg atgaaggcgg acgtgggtta gcgaacgtag atgttgtgcc
    36721 aatcggagaa gaccgttttc ctgtcattac tggaaaaaag aaaatgtttg aatttgatga
    36781 aaatcatttt tatccagcaa aaattgcgat cgatatgtat catcattata aggaagatat
    36841 tgctttattt gcagaaatgg ggttcaagac ttatcggatg tccattgctt ggagtcgtat
    36901 ttttccaatg ggcgatgaaa aagagccaaa tgaatctggt ttgaaatttt atgaggatgt
    36961 ctttaaagaa tgtcaaaagt acgggatcga gccgcttgtg acgattacac attttgattg
    37021 tccgatgcat ttgatcaaag aatatggtgc gtggcgttcc agagaactcg ttggttttta
    37081 tgaaaatctg tgccgagtca tcttcaatcg ctataaagga cttgtcaaat attggttgac
    37141 attcaatgag atcaatatga tccttcatgc accattcatg ggcgcagggc tgtattttga
    37201 agaaggagaa aatgaagaac aaatcaaata tcaagcggca catcatgagt tggttgcaag
    37261 tgccatcgca acgaagattg ctcatgaagt agatccggag aacaaagtag gctgtatgct
    37321 ggcagcagga gcatattatc catacacttg caatcctgaa gatgtttggg cttctagaaa
    37381 atcagatcga gaaaattatt tcttcattga tgtccagtca cgaggagaat atcctgctta
    37441 tgcattgaaa gaaatggaac gaaatggcat tcagatcaaa atggaacctg gggatgccgc
    37501 attgctgaaa gaacatactg ttgattttat ctccttttct tattattctt ctcgagtttc
    37561 gactactgac aacgaactct tggaacagac tgcaggaaat atctttgctt ctgtcaaaaa
    37621 tccatatctg gaagcaagtg aatggggttg gcaaattgat ccattaggct tgagaataac
    37681 gatgaatgat atttatgatc gttatcaaaa accactattt attgtagaaa acggcttagg
    37741 tgcagtcgat acgcccgatg agaatggtta tgtggaagat gactatcgaa tcgactatct
    37801 ggcagcacat atccaggcaa tgaaagatgc agtagaatta gatggagtcg atctattagg
    37861 ctatacgact tggggatgta ttgatttagt atcagctggt acaggtgaga tgaagaaaag
    37921 gtacggattc atttatgtcg atcgtgacaa cgaaggaaat ggcacattag cccgttcgaa
    37981 gaagaaatca ttcaactggt ataaaaaagt aattgattca aatggagaaa atctttcgaa
    38041 tgattgatca atgatttata aaagtctatt gtttttcagc ggatttttcg agtataataa
    38101 agctattcga gaaacgcaag cagaacaata gacttttttt cgcattggct gtaaagtatg
    38161 cttacttgat taagtaaagg agaatctaca tatgtatgat gtaatcgtta ttggcgcagg
    38221 tcctgctggg atgactgctg ctttatatgc ttcacgttca aacttatccg tattgatgat
    38281 cgaacaaggc gcccctggcg gccaaatgaa caatacggca gaaatcgaaa actatcctgg
    38341 tttcgattca ataatgggtc ctgatctagc ttataagatg tatgaaggtg tttcaagatt
    38401 tgggacagaa aatgcttatg gaatcgtgca agatatcaag gatcacggca gccacaaaga
    38461 agttatttgt gaagataaaa cttacgaagg aaaaacagtc attatcgcaa caggctgtat
    38521 ccaccgtaaa ttaggcgtac ctggcgagga agaatttgct ggacgtggcg tttcttactg
    38581 tgcagtttgt gatggtgcgt ttttccgtaa caaacgtcta gtagtgatcg gaggaggaga
    38641 ctcagcagta gaagaagcca tctatttgac acaatttgct tctgaagtag tcatcgtcca
    38701 ccgtagagat gaattacgtg cacaaaagat catccaagac cgtgcatttg cgaatgaaaa
    38761 aatttccttt atgtgggata cagtcgttga cgaaatcgtt gggaatgaaa tggtagtgac
    38821 aggtgtgaaa gcacgtaatg tcaaaaccgg caatgagttt gatttggcag cagacggagt
    38881 attcatctat gttggactgg atccattaac tgcaccattt agagaagcag ggttgacgaa
    38941 cgaagctggc tggattccaa cagacaacga aatgaagacg aatataccag gcgtttttgc
    39001 catcggagat gttcgtgaaa aagatttgcg acaaatcact actgctgttg gtgatggtgg
    39061 gatcgctgga caacaagtgt ataaatactt ggaatcttta gaagaaacaa ctgcataagt
    39121 ttaaaaagag tggaagaacg gtttaattgt tcttttactc ttttttttcg cgtgaaatct
    39181 actgaatccg atttatgcga atacttgcaa ccgtgcttga taaaagaatc ctttcttgat
    39241 ttaagcgttt tttggtcctc atactttcat atctaataag tgaatgctat acttattaag
    39301 aaccatattt cagaaagagg tgttattttc atgtcttggg aacaagtgta tgaacaatgg
    39361 gtacatgaag aaaatttaga agaaaactta aaaaaacaat taaatgatct gggtgaagat
    39421 cctgaaaaac ttgaagatgc tttttatgca ccgttggaat tcggtactgc aggcatgcgg
    39481 ggaatcttag ggcctgggat caatcgaatg aacatctata cggtaagaca agcaacagaa
    39541 ggacttgcac gtttcatgga tacacaaggc gaagaaacaa aacgccgagg tgtcgctatc
    39601 gcttatgact cacgccatca atcaccagaa tttgccatgg aagcagccaa aacattagca
    39661 catcacaata tcccatctta tgtattcgaa agtctacgtc caacaccaga gctttctttt
    39721 gcagtccgtc atttgaagac gtttgcggga atcatggtaa ccgcatccca caatccagca
    39781 gcttacaacg gatataaagt ctacggagaa gatgggggac aaatgcctcc tgcagacgca
    39841 gatgcattga cgaaatatgt ccgtgaagtc agcaatccgt taaaagttga agtattatct
    39901 gacgaagaag ctgaacatag cggattgatc acgatcatcg gagaagaagt ggatgccgct
    39961 tatctagaag aaatcaaagc agtaacgatt gatcatgaat tggtggaaac aatggggaaa
    40021 gatttgaaac ttgtgtacac accacttcac ggaacaggaa aaatgttggg cgaacgtgcg
    40081 ttgaaacaag ctggattcga acaattcgta ctagttccag aacaagctgt cgcagaccca
    40141 gatttcacta cagtcaaatc accaaatcca gaagaacact ctgcttttga gtatgcgata
    40201 cgacttggcg agaaagaagg cgctgatttg ctgattgcaa cagatcctga tgcagaccgt
    40261 ttaggtgcag ccgttcgttt gccaaatggc tcctatgaag tattgaccgg aaaccaaatc
    40321 ggagcactgt ttgctcaata tattttggaa gcacaccaat cgcgaggcac tttacctgaa
    40381 aacgcagcgg tattgaaatc gatcgtttct agcgaattac ctacagcaat tgcggaacac
    40441 tatggggcta ccatgttcaa tgtattgaca ggattcaaat tcattgctga aaaaattcaa
    40501 caatttgaag aagatcattc ccatactttt atgttcggat tcgaagaaag ttacggttat
    40561 ttagttaaac ctttcgttcg cgacaaagac gcgatccaag cattgctctt attagcagaa
    40621 gtagcagctt actataaaaa acaaggcaaa acgttgtatg atggcttgca aacgattttt
    40681 gaaacctatg gctactacgc agaaaaaacg atttctgtga caatgagcgg acaagaaggt
    40741 tctgcgaaaa tctctgcttt gatggaaaaa ttccgtgaag aagcaccaac agaatttgcc
    40801 ggagcaaaag tcatccaaac agaagacttc aaagagttga cgagaacaga ccgtgaagga
    40861 aacgtagaaa aactaacgac accgccatct gacgtattga agtatacgct ggaagaaaat
    40921 ggctggatcg ctatccgtcc ttcaggaact gagccgaaaa tcaaatttta tatcggtgta
    40981 aaaggaaact cttcagaaga cgcagctgaa aaagtagccg cttatgaaac gattatccaa
    41041 aagatgacag aagaataaaa aaacttgaga ctgatggaaa aagtggtaaa ctttagaagt
    41101 cagtcttttt tcaagttgga ggtgaatgga ttgccagaca cgcaaaaaac aaaagtagag
    41161 aaagtcagcc aactattcaa aatgttgagt gaccccactc gtctcaaaat tctgctgtat
    41221 ctgaaagacg gggaacaaaa tgtgacagca atcatccaag cagtggaaat ggaacaatct
    41281 gctgtatcgc atcaactgcg tttattaaga gaaaaccatg tcgtcaagtc ccatcgtgag
    41341 ggaaaagcga ttttgtatag tcttgatgat tctcacgtac tggatatttt gaaccagaca
    41401 ctcaaacatg tggaacatca ataacgatag gtgcaaatag agacaaactg gactgtgtca
    41461 acgaatcttc ttcgctgaca cagtccagtt tttttaaaga aaaattgtta gtctgtttct
    41521 tcggtggttt cagttccata ataactatgg tcttctaggt ctaaagcatt caaaataagc
    41581 gaagctgttt cttcaataga taaagaagcg acattgatta ctacgcagcc caatttttca
    41641 taaagatcat tcgcaaaatc gagttcagct cgtatttttt cgatatcgga ataagcagta
    41701 tcaggattta atccgtaaga gcgcatccgt tctttacgga tattgtttaa aatatctggg
    41761 ttattcgtca gtcccacgat ttttttagga tcgatctccc aaagctgttt gggaatatgt
    41821 gcttgtggga tcaaaggtag attggcaact tttaaatttt tgttagctaa aaacaagctc
    41881 aaaggcgttt tggaagttcg ggatacgcct agcaaaacga catctgcttc tagaaaccca
    41941 cgaggatctt tcccgtcatc gtattttaca gcaaattcca ttgctttgat ccgtttgaaa
    42001 tagttctcat tcaataaatg aagggcgcca ggttgccgaa tgggcttgat accagttaag
    42061 cgctcgattt ctgatatcgg cggagtcatc acatcaaaac taaataattg atgttcttca
    42121 caaaaagaac gcgtcatctc gatcaattct tgattgatca atgtgtgcag gacaagagct
    42181 ttttcttcta gtgcatcttg cagtgcctgc atcaatgttt tctcatctgt cacaaatgtt
    42241 ctgcggaata aattgaactc aacgctagga tattgggcca tagtggcttg cgctagtttt
    42301 gtcgcagttt ctccagcaga gtcggagatg acaaaaaaag taacgatttc tgtagaatcg
    42361 gaagacatgg ataaaacctc acttgcattt tttttatgtt tattatacca tactctagaa
    42421 tgtgtacttc gtaatcattt tgatttggag gaataagtca tgaaaaaaca gacgttgatc
    42481 gaacaatcac tagaaacatt gaaaaataac ggttttaaat atactaaaaa aagagaagca
    42541 ttgttaactt atttagtgaa gcgcaatcgc tacgtatccg ctaaagaagt ttttgactat
    42601 atgaatcagg aattcaaagg agtaagttac gatacgattt atcgaaatct ccatgatttt
    42661 gcagaaatcg atttactgga agaaaccgat ttaaatggag agatgaagtt tcgttttcat
    42721 tgctgtcaag gggaaatcgg ccatcaccat cactttatct gtacggtgtg cggaacaacg
    42781 aaagaaatcc atatgtgtcc aatgaattat ttcgaagaac agctcggtgg atgtacgatc
    42841 gaagggcata gattcgagat tttcggaaga tgtgaaaatt gtcaataagg atttaaataa
    42901 aataacattt gataatttta aaaaaaagca agatttcctt gacggtcaaa acagcattag
    42961 atataatatt tacaggacag tcttgttttt tgagatatat ttcaataaca attacggtta
    43021 ctttttaagc tcggagggag ggaattaaca tgtcaaaaac agtcgttcgc aaaaacgaat
    43081 cacttgacga tgctcttcgt cgcttcaaac gttccgtgtc aaaagcgggt actttacaag
    43141 aatctcgtaa acgtgaattc tacgaaaaac caagtgtaag acgcaagaaa aaatctgaag
    43201 cagctagaaa acgcaaaaaa ttctagttct gtgatgaatt ggagttggta gtgtgtcact
    43261 actaagtaga ttaaacgatg acatgaaaac agcgatgaaa gcgaaagata aggaatcttt
    43321 acaagtcatc cgaatgatca agtcatctat acaaaacgag cagattaaag aaggtcacga
    43381 cttgacagaa gaagaagaat taacggtgtt gtctcgcgaa atgaaacagc gacgtgattc
    43441 tttgcatgag tttgaagaag caggacgtga cgatttagct gaaaaagtaa aaagtgaaat
    43501 tgtgatagtc gaaaaataca tgccggaaca actttccgac gaagaaattc gacaacttgt
    43561 tcaagaagca atcacgcaaa ctggtgcttc atcaatgaaa gaatttggca aagtcatggg
    43621 tgcgatcatg cctaaagtca aaggaaaagc agatggaaac caagtaaatg ccattgtgaa
    43681 agaactatta caatcttaat tttatcattt aaactgcgga caatcattgt ccgcagtttt
    43741 tttgtgctcg taaaaaagag atctaaaaaa tagctagacg aaagaatagg attctttcat
    43801 ctagctgttt ttttcctgtt taatatttct tatgttaaat tgtgtttgat ctggttattc
    43861 ttctggtagt tcatttgctt cttgtttata ccctgatagg cgataggcgt aatagaagag
    43921 taagctgcca gcatacccaa gtaacgttgg gaccatccag ccaagtccca atgcagcaaa
    43981 aggaacgtga tgatgataaa attcgagcat gcttgctaca actctttgtt gactcactaa
    44041 agcaggcgaa tttgccagca tatccaatac ggcgggaaca gcagcaaaca acatcgttac
    44101 ttgatagaca aatggtgtct tttggaaaaa ttttgctgtt aaggaaagca aaatcaacgc
    44161 aagcgacaat ggatacaaaa gcatcaatac tggtaaagac cattggatga tattgtccaa
    44221 accagcattt gcaacgacaa aagagacgaa agtcgttaac cgcagccatg tcatataact
    44281 gactttaggg aacagtttat ggaaatcttg tgcaaaagaa acaacgagtc ccatagcggt
    44341 agtgaagaca cctaatgtga ccatcactcc taagaagcta gcgccgaagt tacctaaata
    44401 aaaattagtg atctgagaaa gtgcagttcc gccattttct gataaagaca tcttattcaa
    44461 actcatcgca ccgagaagaa caagaccaaa atagatcatg acttctagcg caacgctcag
    44521 gctaccagct tttgctgtta attctgtgac ttttttatct ttgaaaccaa ggtcacggac
    44581 tgcatggaca aaagtgacac ttaaggcaag caaggctact gcatcaaccg tgttataacc
    44641 ttctaggaca ccatttgtta aagcgtttgt ttggtaagta tctgcagcag attgatttag
    44701 atttcccatt gggtggaata aagaaacgac aaaaatgatt gctaataatg caagaaacaa
    44761 ggagtttaga tactttccga cgtatttgac caaccgtgtg ggatggacag taaggaaata
    44821 agcaagtaaa aagaaaagag cagagaataa aaacatacct aatgaattat atttttcagg
    44881 caaaaatggt ttagcagcca tttcaaaagc agtagcagct gtccgtggtg taccgaaaaa
    44941 tggtccgatc gtcaaatgaa caaggattaa gaacaaggca gcataccatt ttccgacagg
    45001 tttagctaag tcatacagac catcactttt tgtcactact acagctaaga tagctagtaa
    45061 tgggaaaaga gcgcctgaga tggcaaatcc tacgccagca ctgacccagt ttgcgccagc
    45121 catttgacct aagtggacag gaaagattag attacctgac ccgaaaaaca tcccgaagat
    45181 aagtgaactg accatcaata gttgtttcca tgaataagtt tttgtttctt ccataaattt
    45241 ttcctccaat aattatcatt ttgattgacg atataaacgt tgcagcaatt agtaatgcca
    45301 ctaatatcag attccatgaa aaccaccacc ttaaaatata aaaaaaagcc cttttccact
    45361 tgggaaaagg gctctagatt ctagaacggt accacctttt ttcgcttaaa aaagcttctt
    45421 cattcacgga cgctttttga taaagttatc cgctagcctg ataatggagg cagccacaat
    45481 gatctacgtt ttcgacattg actcggaaat gatgttcgtc tattcgattt attgtcattt
    45541 ctcacttaca tgactcactg ggaataaatc gttgaatagc ctactgtttt cctcgacgct
    45601 ttgctgtatg tttatttgtt gacgtaataa tacacctgtt ttttttgaaa tgcaagaata
    45661 ttttaactat tttgttttct ttttttgaaa caagaaaaat acctagagaa tacctctgaa
    45721 tgaaacggaa tattttgttc tccactttca tataacttac attctcagat tctttttatc
    45781 aaaacgagac ctttagcgaa tggaaacagc tattttttaa cgtttcttgg tacgtatggt
    45841 taaacgactc ttttacaacc ttttcttatt tgctatatag tttcttctga gtatggtaaa
    45901 ataggaaaga atgaatcgaa aaaaagggga tgtctatttt tgacggaaga gtctagttct
    45961 ttagagatta gattgacagc tgctgatgat gtcagcatgc ttttaggtgc acacgacaag
    46021 catataaaac tgatcgagga aacaacggaa acaaccatcc atacacgtgg agaaatcatt
    46081 cagatcattg gtgaacaatc ccagatttcc ttggctgcct cagtcattcg gacattacag
    46141 gaattgatca aacgaggaat ccacgtaagt acaccagatg tcgtgacggc attgaaaatg
    46201 gggcaaaaag gtacattaga ttattttgtt gagatgtatg aggaagagat cgtcaaagac
    46261 cgtaatggaa aacctatacg ggtaaaaaac agcggtcaaa agaaatacgt tgaatcggta
    46321 gccaaacatg acatcgtatt tggtgtaggg ccagccggaa cagggaaaac gtttcttgcc
    46381 gtagtattag caattgctgc cctaaaaaaa ggcgaagtgc aaaaaatcat tttgacgcgg
    46441 cccgctgtag aggcagggga aaacctaggc tttctacccg gggatctgaa agaaaaagtg
    46501 gatccttatc tgcggcctgt ttatgatgct ttataccaaa tttttggatt ggatcatacg
    46561 aaccgattga tggaacgagg cgtaatcgaa atcgcaccat tagcttacat gcgggggcgg
    46621 acattggatg atgcattcgt tatcttggac gaagcacaaa acacgacagt cgctcagatg
    46681 aaaatgttct tgacccgtct cggttatcaa tcaaaaatga ttgtcaatgg ggatacaagt
    46741 cagatagact tgcctcgagg gacgacaagt ggtcttgttc atgcagaacg aacactaaag
    46801 caaatcaaaa aaatcgattt tgttaatttt gaagcaagtg acgttgttcg gcaccctgta
    46861 gttgccgaaa tcatcaaagc ttatgaaaag gcggatcttc atcaagaata agctttgctt
    46921 aggaggataa gcacatgttg aataaaacac tgcagaaact acaagaaaaa ctcggaaagg
    46981 cagcattgcc tttccttctg ttcttgtttt ttatcattgt ctgcggcgtt acatttagta
    47041 gtgtcaaaca aaaggccaat gatttcagag aaggccaagt agcagaagag agtatccgtg
    47101 cgaataaaac gatcgagaac acggaagaaa ccgaacaaag aagaaaactg gcagcagaag
    47161 cagtgactcc agaatatact tatcaaaaag atctggcaga tgaccagaac aatcggatca
    47221 agcaattatt cgaattgatc gataagacga atgataccat caacaaatct tataatgaca
    47281 aggtagataa ggcgaaggaa gatgaaaata tcccgaaacc tactacagaa gagcggattg
    47341 ctacactgaa gaaaaatttt gaaaacgtga atgcagaaaa cgttgcgttt taccaaaaac
    47401 tgccgaacaa tttttattcg acgatatttg aaatgactga aacggaaatt cacacggtta
    47461 gggatgaaag tttgaagctc attgacgaac agatgagtaa acaagttcgt gaaagcgaat
    47521 tggaagcttt taaacaagaa gcagaagatc agattcagta tttgaatgtc acaacagagc
    47581 agcaacaaat gattcgctac ttagtggatc aaggcattgt agtcaatgat gtattgaatg
    47641 agaaaaagac agaagaatta aaacaatctg cacgagaagc tgtacaacca gtgatgatct
    47701 tccaaggaga aatcattgtt cgcgaaggaa atcaaattga tgcttcagcg atgaaaaaat
    47761 tggaattact aggattgacg aatcaaacga cttcgatttt cccactcatt gccatggttt
    47821 tagctgtttt gcttcaaatc ggcgtactga tttataattc acttcaattc caaaatgcag
    47881 gagaaagaac aaaatacgtg cttttctatg tgacagctat gtcagtcagc atagtgctga
    47941 tgaaattttt ccaattattc caaacggaac aagctactta tatcccgctg ttttatcccg
    48001 ctgcttttgc accacttgtt ttaagctttt tcttgaaccg aagagcaggg atcatggctg
    48061 gtattttcca agcagtatct gcacttttca ttttttatga ttcgattggc acgaactttc
    48121 taacgattat cttgatgtct tatgttttct ctgggctcat ggcaacgatt gtcagacgca
    48181 aacggatctc agaacaagga ttatctgctg ccatgtgggt cattattttt cctgtgatga
    48241 tggatgtcat tttgatcgtc tatcaaggaa tgagctttgg tgatgcaacg acttggttaa
    48301 tgcttgtctg cgggttcact ggtgcactct tctcattctt gctgacgatc ggtctacacc
    48361 catatattga attgatggtc aatgatgaca gtgtaattgt attaaatgaa ctcagcaatc
    48421 caaatcatcc tttattgaaa cagttgctgg aagaagcgcc aggtacttat catcatagta
    48481 tgatggtagc taatctaagt gctaacgctg tagcagaaat cggcggacgt tctttgctaa
    48541 ctcgagtagc ttgctattat catgatatcg gcaagatcaa acatgctagc tttttcgttg
    48601 agaacttgcc ttcaggagcg gaaaacccgc acaatttctt attgccagaa gacagtaaac
    48661 atattatctt tggacatgtg acagatggag ccaaaatatt ggaagattat aatatgccgc
    48721 aaatggtgat cgatatttgc aaacagcatc atggaaccac gctaatgcgt tatttctaca
    48781 tcaaagcgaa agaacgcaat ccggaagtaa cagaagagca attccgttat cctggaccaa
    48841 aaccgcaaag tcgtgaagca gggatcgtca atattgctga cagttgtgag gctgcagtac
    48901 gggcaatgga tcatccgacg agcgaaaaaa tcgaaagttt tgtccataat cttatcgaag
    48961 agcgtatttc cgatggtcaa ttagacgata gtggactgac cttgaaagaa atccgaaaag
    49021 tggaaaaatc attgatcagc ggtttaagta gcaccttcca ttctcggatc aaatatccta
    49081 aaatgaaaac agaagttgag aagataaagg aagaacaaga agaatagaaa gaagcgatgt
    49141 ataatggata ttacctttat ggatgaaaca aaccaagtct cagaagagaa aatgaaagaa
    49201 atcgatgact tgctgcaatt tgcagcatct tatttaaagc tgccagagga aacagaaatg
    49261 tctgttactt tcatggacaa tgcggcaatc caagtaatca atcgtgatta ccgtggaaaa
    49321 gatgcaccga cagacgtcat cagttttgct ctggaagaag aaggcgaaga cgaaatccca
    49381 gtgatttttg aagatggaga gaatcctttg cctcgtgtac taggagacat catgatttct
    49441 attgaacgtg ctgctgaaca agcagaagct tacggccatt catatgatcg ggaacttggc
    49501 tttttagcgg tacatggatt cttgcatatc aacgggtatg accatatgac accagaagat
    49561 gagaaagaaa tgtttggttt acagaaagag atcttggatg cctatggact caaaagataa
    49621 aaacgtagag aaaaacaaac atttcctgat gtctgtggaa tttgctgtcc aaggagtcaa
    49681 aaccgttttt gaggaagaac gcaatatgag gaaacatgtt gcgttcggta tccttgcttt
    49741 atttatgggc gtgattttcc agcttgatcg gatagagtgg ttatggctgc ttttagctgt
    49801 ttttctcgtt tggatagtag agatcatcaa tactgttttt gaaaatgtag tggacatgtt
    49861 tacggatttt cattttcatc cgattgggaa aaaaatcaaa gatatggcag ctggagcagt
    49921 tttagtgacc gcttgttttg ctgtcatcat tgggttgatt ttatttgtcc ctaaaatata
    49981 tcatttgttg tttaattaaa aaggagaacg tatatgccag aacataaatc agggtttgtc
    50041 gcaatcgtcg gccgcccgaa tgtaggaaaa tccacattat tgaaccgaat cgtgggtcaa
    50101 aaaattgcca ttatgagtga caaggcacag acaactagaa ataaaatcca aggagtatat
    50161 actacaccag aagctcagct gatttttatc gatacaccag gaatccataa accaaagcat
    50221 cgtctaggag attttatggt agaagcagct tacagtgcgt tgaaagaagt ggatgcagtc
    50281 ttatttatga tcagtgctga tcaaaaacgc ggccggggag acgattttat tattgaacgt
    50341 ctaaaaaacg tgcattctcc agtttatctg attatcaata aaatcgataa gatccatcca
    50401 gacgagttgc tgggaatcat cgaagattat tcgacgcaga tgccttttgc acaagtgatt
    50461 ccgatctctg ctacagaagg aaacaatgtc gaacgcttaa tggatgtatt agtcagtgaa
    50521 atgccagagg gcccacaata ttttccagat gatcaagtga ctgatcatcc tgaatacttt
    50581 atcgtttctg aattgatccg tgagaaagtc ttgtttttaa cacgagacga agtgcctcat
    50641 tctgtcgcag tcgttgttga ttcgatgaag cgaaacgaga atgataaaat ccatatccag
    50701 gccacgatca tcgtcgaaag agatagccaa aaaggaatca ttatcggcaa aggcggaaaa
    50761 atgctgaagc aagtaggaac gaaagcaaga aaggatatcg aaaatctttt aggagacaaa
    50821 gtatttctag aactttgggt caaagtccaa aaagactggc gagacaaaaa agtctatctg
    50881 caagactttg gctaccgaaa agacgaatac tgatattctg aacttcttat aggttaagta
    50941 tagaaataaa ctggataaaa atccgcacgt tgagaaaaac ggcggaagat cgaaacataa
    51001 ataatctgga tcggcagaat ctgcttttcc agatttattt tttaggaggg agaatatgag
    51061 ccagcttgca gaatcgaaag gcttgatttt atctagccgt aattacaaag aaaaagataa
    51121 attagtcaaa atctttacgg aatcttctgg caaaatgatg ttttatatca aaggaatcca
    51181 tcggcaaaat cagccattag ctccggcttt gctccccttt accgaagcag tctatatcgg
    51241 taattttcga agtgaaggtt tgtcgttttt aagcagtgtc aaagacgtcc atgcgtttcg
    51301 gaacatccaa gaagatatct ttttgagcgg ttatggtacc tatttgctaa atctagtaga
    51361 tgcagcaatc gaagatcatc aatatgaccc gcatttattc caatttacgc aacaagctct
    51421 gcagcggatg gatcaaggag atgatgcaga aatcatcacg aatatctttg aagttcaaat
    51481 actgcaacgc ttcggaatcg cgccaatttg gacgcactgt gtcgtatgcg gggaaacaaa
    51541 aggaaaattt gactattctt ccaaatctgg cggtgtgata tgcgagaaac actggccaat
    51601 ggacgaacac cgttatcatg cagatcctcg cgctgtttat ttcgttcgaa tgttttcggc
    51661 tatttcttat gataagatca gtggaatcca cttgaaagaa gaaacgaagc aggcaatccg
    51721 tgacctcatt gaccagttgt atgatgaata tgtcggtatt catctaaaaa gcaaaaagtt
    51781 cattgatcaa atgaagtctt gggcacacgt attaaagacg ccagagaaaa atatgcctga
    51841 aaaagagata cctgaaaaag aagaataaat gaaagataca acaaaaaaat caaaactatt
    51901 tgacaaatac agggacttcc gtctattatt aaccgtagat taaatagatc gttcactgag
    51961 aggatctatt atataaacaa gaaaactgca aaatgaatac gtacattgac caagatcagt
    52021 aaaaaacacg tggtttagcg agttcgggag agtggaagcc ggacccaaac gatttttgaa
    52081 gaggcacttg cgagtacgat aaatgaagct gccgaatctt tattcggaag tagggtggaa
    52141 ccgcgaaatt attcgtccct atgtctgtaa tcagacatag ggactttttg tcgttttctt
    52201 tcctgtattc ttattgacag caacgcttaa aaattgaaag gggcaaaaca gatgagtaaa
    52261 aaattgaccg tccaagatat gattttaaca ttacaaaaat tttggtcatc aaacggctgc
    52321 atgctgatgc aagcttatga cacagaaaaa ggagcaggta cgatgagccc atatacattc
    52381 ttacgggcaa tcggaccgga accatggaat gctgcttatg tagagccatc ccgccgacca
    52441 gctgacgggc gttatggaga aaacccaaac cgtttgtatc agcatcatca atttcaagtc
    52501 gtaatgaaac cttcacctga aaatatccaa gagctttatt tagaaagctt gcgtctttta
    52561 ggaattgatc cattagaaca cgatattcgt tttgttgaag acaactggga gaacccatcg
    52621 atggggtgtg ccggtgtggg ttgggaagta tggcttgatg ggatggagat cacacaattc
    52681 acgtacttcc agcaagtcgg aggactacct tgtaaaccag ttacttctga gatcacttat
    52741 ggactagaac gtcttgcttc atatattcaa gaagttgaaa gtgtctatga tctggaatgg
    52801 gctgatggtg tgaaatatgg cgagatcttc aaacaaccag aatatgaaca ttccaaatat
    52861 tcttttgaag taagtgacca agacttgcta ctaagcaact ttgaccgttt tgaaaaagaa
    52921 gctaaacgct gtatcgatga aaatcttgtt catccagcgt atgattatat tttgaaatgc
    52981 agccatacat tcaatttatt agatgctcgc ggtgcagtat ccgtaacaga acgagcaggt
    53041 tatctttccc gtatccgcaa catggctcgt gctgtagcca aaatctttgt ggcagaacgc
    53101 gaaaaattag gattcccttt attgaataaa aacacagcaa atccagaaaa catgaaggag
    53161 gctgaataag atggcaaaag acttattact tgaaatcggg ctggaagaaa tgcctgctca
    53221 tgttgtcaca ccaagcatga aacaattaga agaaaaaaca gcaaaatttt tagatgaaaa
    53281 ccaattgact tatgatacga tcgaaacgtt ttctacaccg cgccgcttag caatcaaagt
    53341 aacaaacatt ccagaacgtc aagcagacag cgaagaagaa gtcaaaggac cagccaaaaa
    53401 aattgctttg gacgcagatg gaaactggac gaaagcagct gaaggatttg tccgcggaca
    53461 aggtctgacg acagatgcta tcacttttag agaattaaaa ggcgtggaat atgtctatgt
    53521 aacgaaatac actcacggca aagaagcaaa agaagtattg acaggtttag cagatgtcat
    53581 taaaagcctc actttcccag taacgatgca ctgggcagaa tacgattttg aatatattcg
    53641 ccctattcac tggatcgtgg cattgttaga tgacgaaata atcccgtttg agatcttgga
    53701 tgtcaaaaca gacagagctt caagaggtca tcgtttctta ggcgaagatg tgacatttac
    53761 gcatgcaaaa gaatacgaag aaaaattgaa tgaacaattt gttattgctt caccgcaaaa
    53821 aagaaaagaa atgatcatca cacaagcaaa cgaattagcc gaaaaaaatc actggatcat
    53881 tgatctagat gaagatttac tagaagaagt caacaattta gtcgaatatc caactgtctt
    53941 ttctggcgac ttcgaagaaa aatatctatc tgttcctgaa gaagtactag tcacttctat
    54001 gaaagaacat caacgttatt tcgaagtacg gaacgatcaa ggtatgttgc ttcctcattt
    54061 tatttctgct cgtaatggcg atcgggtaaa attggaaaat gtggtacgag gcaatcaaaa
    54121 agtattgacg gctcgtttag aagatgcgga attcttttat aatgaagaca agaaagcttc
    54181 aatcgaccaa tacgttgaaa aattaaaaac tgttactttc catgaaaaaa tcggtagcat
    54241 ctatgagaaa atgcaacggg tacaagtcat cgctcgcatc ataggcgaac aagtaggact
    54301 atctgaggaa gaattgatgg atctgcaacg cgctgctgaa atctataaat ttgatctagt
    54361 aaccaacatg gtaggagaat tccctgaact acaagggatc atgggagaaa aatatgctct
    54421 gctgaaaggc gagaaaccag ccgtagcaac agcgatccgt gaacattaca tgcctatatc
    54481 cagtgaagga gatttacctg agactacagt aggttctgtt ttagcgattg ctgataaatt
    54541 agatagtatc ttttctttct ttgcagttgg gatgatccca agtggctcaa acgatccata
    54601 tgctttgcgc cgtcaagcat atggtgtcgt tcgaatcatc gaatcgaaaa aatgggcctt
    54661 tcctttgtct gttctgcaag aaacgattag tgaagttatc agtaaagata cggatcgttt
    54721 tggcattggc ttgtcagcgg gacaacaaca ggtgatcgac tttatcaaag gccgtctgcg
    54781 ccaattattg acaacgaaga atatccgtca tgatgtcatc gaagctgttt tgaatgcaga
    54841 acaaaaagat ctgacaaaag tatttgcagc agcacaattg ttcaagcagc atcttgcaga
    54901 tgaagacttc aaaccatcga tggaagcttt gacacgtgtc gtgaacttag cgaaaaaagc
    54961 ggagcttgaa aaacaatcag aagtagaccc tgaactgttc gaaaacgaag cggagaaaga
    55021 actgcacaaa gcagtagagg caatgaaaac aagctttgct gaccaaacga tcaacgaaaa
    55081 ttatgatcag cttgtaagcc ttcgcccact gatcgaaaat tattttgatc agacgatggt
    55141 catggcagaa gaaccagcag tacgtaagaa ccgattaacg caattaagtc aaattgctgc
    55201 catggctttg tcactagcaa gtttagatca aatcatcaca aaataatttc taatatgtaa
    55261 ataaaccaga gaacaggcac aaaataggct atttctctgg tttat
//
LOCUS       DACCYH010000008        50001 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.008, whole genome shotgun sequence.
ACCESSION   DACCYH010000008 DACCYH010000000
VERSION     DACCYH010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.008"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(2..1012)
                     /locus_tag="IVY85_000456"
     CDS             complement(2..1012)
                     /locus_tag="IVY85_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8043495.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1192..1536)
                     /locus_tag="IVY85_000457"
     CDS             complement(1192..1536)
                     /locus_tag="IVY85_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8043496.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(1529..1963)
                     /locus_tag="IVY85_000458"
     CDS             complement(1529..1963)
                     /locus_tag="IVY85_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP8043497.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            2101..2838
                     /locus_tag="IVY85_000459"
     CDS             2101..2838
                     /locus_tag="IVY85_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8043498.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            2831..4042
                     /locus_tag="IVY85_000460"
     CDS             2831..4042
                     /locus_tag="IVY85_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8043499.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            4249..5028
                     /locus_tag="IVY85_000461"
     CDS             4249..5028
                     /locus_tag="IVY85_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP8043500.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            5098..5760
                     /gene="trmB"
                     /locus_tag="IVY85_000462"
     CDS             5098..5760
                     /gene="trmB"
                     /locus_tag="IVY85_000462"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP8043501.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5858..7612)
                     /gene="ade"
                     /locus_tag="IVY85_000463"
     CDS             complement(5858..7612)
                     /gene="ade"
                     /locus_tag="IVY85_000463"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP8043502.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            7744..8673
                     /gene="cpaF"
                     /locus_tag="IVY85_000464"
     CDS             7744..8673
                     /gene="cpaF"
                     /locus_tag="IVY85_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP8043503.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8630..9679
                     /locus_tag="IVY85_000465"
     CDS             8630..9679
                     /locus_tag="IVY85_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP8043504.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            9676..9954
                     /locus_tag="IVY85_000466"
     CDS             9676..9954
                     /locus_tag="IVY85_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP8043505.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            9929..10399
                     /locus_tag="IVY85_000467"
     CDS             9929..10399
                     /locus_tag="IVY85_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP8043506.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            10416..10718
                     /locus_tag="IVY85_000468"
     CDS             10416..10718
                     /locus_tag="IVY85_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043507.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            10735..11106
                     /locus_tag="IVY85_000469"
     CDS             10735..11106
                     /locus_tag="IVY85_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP8043508.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            11078..11437
                     /locus_tag="IVY85_000470"
     CDS             11078..11437
                     /locus_tag="IVY85_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043509.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            11609..12616
                     /locus_tag="IVY85_000471"
     CDS             11609..12616
                     /locus_tag="IVY85_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8043510.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            12641..13825
                     /locus_tag="IVY85_000472"
     CDS             12641..13825
                     /locus_tag="IVY85_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP8043511.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14262..15845)
                     /locus_tag="IVY85_000473"
     CDS             complement(14262..15845)
                     /locus_tag="IVY85_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8043512.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(16423..16566)
                     /locus_tag="IVY85_000474"
     CDS             complement(16423..16566)
                     /locus_tag="IVY85_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP8043513.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(16789..17268)
                     /locus_tag="IVY85_000475"
     CDS             complement(16789..17268)
                     /locus_tag="IVY85_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8043514.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(17341..17625)
                     /locus_tag="IVY85_000476"
     CDS             complement(17341..17625)
                     /locus_tag="IVY85_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043515.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(17703..17897)
                     /gene="ssrS"
                     /locus_tag="IVY85_000477"
     ncRNA           complement(17703..17897)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IVY85_000477"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(17969..19255)
                     /locus_tag="IVY85_000478"
     CDS             complement(17969..19255)
                     /locus_tag="IVY85_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP8043516.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            19393..20064
                     /locus_tag="IVY85_000479"
     CDS             19393..20064
                     /locus_tag="IVY85_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP8043517.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            20030..20518
                     /locus_tag="IVY85_000480"
     CDS             20030..20518
                     /locus_tag="IVY85_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP8043518.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            20531..21478
                     /gene="prmA"
                     /locus_tag="IVY85_000481"
     CDS             20531..21478
                     /gene="prmA"
                     /locus_tag="IVY85_000481"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP8043519.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            21479..22243
                     /locus_tag="IVY85_000482"
     CDS             21479..22243
                     /locus_tag="IVY85_000482"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP8043520.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            22476..24689
                     /locus_tag="IVY85_000483"
     CDS             22476..24689
                     /locus_tag="IVY85_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP8043521.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            24700..25146
                     /locus_tag="IVY85_000484"
     CDS             24700..25146
                     /locus_tag="IVY85_000484"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP8043522.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            25317..26576
                     /locus_tag="IVY85_000485"
     CDS             25317..26576
                     /locus_tag="IVY85_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP8043523.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(26665..27612)
                     /locus_tag="IVY85_000486"
     CDS             complement(26665..27612)
                     /locus_tag="IVY85_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8043524.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            28006..29298
                     /locus_tag="IVY85_000487"
     CDS             28006..29298
                     /locus_tag="IVY85_000487"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP8043525.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            29313..31082
                     /gene="aspS"
                     /locus_tag="IVY85_000488"
     CDS             29313..31082
                     /gene="aspS"
                     /locus_tag="IVY85_000488"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP8043526.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            31191..32066
                     /locus_tag="IVY85_000489"
     CDS             31191..32066
                     /locus_tag="IVY85_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8043527.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            32332..34215
                     /locus_tag="IVY85_000490"
     CDS             32332..34215
                     /locus_tag="IVY85_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8043528.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(34290..34574)
                     /locus_tag="IVY85_000491"
     CDS             complement(34290..34574)
                     /locus_tag="IVY85_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8043529.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(34564..35298)
                     /locus_tag="IVY85_000492"
     CDS             complement(34564..35298)
                     /locus_tag="IVY85_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP8043530.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            35427..36050
                     /locus_tag="IVY85_000493"
     CDS             35427..36050
                     /locus_tag="IVY85_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP8043531.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            36242..37363
                     /locus_tag="IVY85_000494"
     CDS             36242..37363
                     /locus_tag="IVY85_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP8043532.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            37370..40498
                     /locus_tag="IVY85_000495"
     CDS             37370..40498
                     /locus_tag="IVY85_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP8043533.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            40517..43711
                     /locus_tag="IVY85_000496"
     CDS             40517..43711
                     /locus_tag="IVY85_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8043534.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(43857..44564)
                     /locus_tag="IVY85_000497"
     CDS             complement(43857..44564)
                     /locus_tag="IVY85_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8043535.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            45329..45727
                     /gene="spxA"
                     /locus_tag="IVY85_000498"
     CDS             45329..45727
                     /gene="spxA"
                     /locus_tag="IVY85_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP8043536.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            46059..46712
                     /locus_tag="IVY85_000499"
     CDS             46059..46712
                     /locus_tag="IVY85_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP8043537.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            46938..47936
                     /locus_tag="IVY85_000500"
     CDS             46938..47936
                     /locus_tag="IVY85_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP8043538.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            48112..49920
                     /gene="pepF"
                     /locus_tag="IVY85_000501"
     CDS             48112..49920
                     /gene="pepF"
                     /locus_tag="IVY85_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP8043539.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        16178 a         8741 c        10454 g        14628 t
ORIGIN      
        1 tttattttga tgaatcagtt gtttcttcag ttgaagattc ttttgttgaa tcagttgatt
       61 ttgtgtcaga actctttgtt gaagctgtag tttcacttga gtctttcgta cttgatgaag
      121 ttgttgtgaa tgcactcaac acattttcaa aggcatcatc tttgattttt acatttgcat
      181 cttttaattc ttcaccgatt actttagttg tgaatgcatt gtcacttagt ttagtttcag
      241 tagcgatatc ttttagttga tctttgtatt tatccatgtc attgccttta ttttggtttt
      301 tcaccatttt gacaacgtag tattctgtcg cataagaagt aggatttgtt gtagtaataa
      361 catcggagat ctcaccgtct ttcaatttga aagctgcttc ttttacttct gcaggaatag
      421 ttgttgtcgt tgagtcgaat ttaactttac cgccatcttc ttttgtctct gtatcagttg
      481 atttgtcttt ggctaattta gagaaatcat ctccatcatc cgctgatttt ttcacatctt
      541 ttgcttcatc ttcacttgat aatttgatga tttgtgcttc tacttctgga tggaaagatt
      601 tccaagcagt tttcaaatcg tcatctgtaa tatctacgtg agctttcaag ccagcttcaa
      661 aagctaaatt gttacgaata tattctttat aagtatcttt cgtatatcca gcagcttcta
      721 attgtgattc aaaagtatca cctaatgatt tcgcttgttt gtcgtattct gcatccactt
      781 gcttatcggt tactttatcg ccatatttgt tattgaatac tttataaatg atcatccgtt
      841 gtactaagga ttggttcgat gattcaagtt ttgcttcatc atagaaatca gatacagtga
      901 tcgttccgcc tttcatcgtt gcgatatctt tattagtatc gcctgaacaa gctgctagag
      961 ttaaaacagc caacgcacta gtagctgcca aaataattga tttttttttc attaaaattt
     1021 gcactcctgt aattagttat tatttttata tattaactat accatatccc atttccttta
     1081 aaaacgttgt cgtggtagtt tcaaacaaaa ttcaaaattt catcacattc ctaggaaata
     1141 cgcaaacttt tctcacttat atagttaaat ctgttgagaa aataatcatt attatatttg
     1201 ttcaggatac tctggcagat cagcttttag tttttctact tgttcattga tttgtgtgat
     1261 acgtggttcg gcttggaatt taaaatcttc gatatctttt ttgattgctt cttggaaagc
     1321 aggaatcaca gattcagctg tttcttttgt ttcgatcaat gcgcttttaa aatgattcag
     1381 gctgttattt aaatcatttg tcagagttgt tgcttcttcc aattcttcga tcagtttctt
     1441 tcttgtttca ttcccgctcc tgggcgccaa taacagacct ccgacagtgc cagcagcagc
     1501 tccaaataac agtcctttga aaaagttttt aatcatcgca ggacacctgc tttacgattg
     1561 tttcttgaat agcttttagt tcttccgatc cataactatc ttgatgattg acaaagtgaa
     1621 tagaaaaatc atcctctttg ctgtagcgag ggactaaatg gatatgtgaa tgaaaaacag
     1681 attgataggc tagttcacgg ttgttattca agatattaag acctttcatt tctggaaaag
     1741 ctttttccaa tgcttgagca atcttaggca cacgcgaaaa aacctctcct gcaagttcgg
     1801 gatcatattc aaaaatatct gctacatgac gtttaggcac aactaatgtg tgtcctttcg
     1861 ttgcttgaga gatgtctaaa aaagcataga ccttgtcgtc ttcatatatt ttatagcttg
     1921 ggatttcttg attgatgatt ttgcaaaaaa tacagttttc catataaaac atcctttcta
     1981 taaagaataa atacttcagc ttaactttac cataagttaa agctattgta aggaaaaaaa
     2041 caaaataccg tcattttctt cgtggtataa taaataaaaa taaatggcgg aggaaaaaga
     2101 atgagtttaa ttatcgatca tgtcacaggg gggtacggac atctccctgt gttaaaagat
     2161 atcagtttcg atgtacagcc tggagagata gtagggttga ttggactgaa cggagcaggg
     2221 aaaagtacga ccatcaagca cattatcggt ctgctggaag cttcaaaagg aacgatagaa
     2281 atcaatcatc tatccttaaa gaaaaatacg gaagaatatc gcaaacaaat tgggtatatt
     2341 ccagagactc cttctttata tgaagaattg acattgagag aacatatcga agtcactgcg
     2401 atggcctacg atatacctat ggatatagca atgaaaagag cagaaaagtt gttggagact
     2461 tttcggttat ctaatcgtct tgactggttc ccagctaatt tttcaaaggg aatgaaacag
     2521 aaagtgatga tcttatgcgc ctttttgatc gagccttctt tatatatcat cgacgaacca
     2581 tttttaggct tggatccact agctatcaat gcattgcttg aattgatggt agaaatgaaa
     2641 aaagaaggag ccggtatttt gatgtctacg catattttag ctactgcaga aaaatattgt
     2701 gatcgttttg tggtcttaca taacggagag atacgagcag acggttctct tgagatgttg
     2761 agaaaagaat ttcagctgcc ggattcttct ttggacgaaa tctatattgc tttaacaaag
     2821 gagcaggcac atgagtagtt tttttcaaaa acgtttagca agacatcaaa agaaaatgat
     2881 gaaatatatg cgttatgtat tgaacgatca ttttgtttta gtttgtcttt tcttgattgg
     2941 cggtgtggga ttatattatt ctaattggtt gaaaaccctg cagccaccgt ttgcaattgg
     3001 agggctcata atctctgtat tttggatgct ctgtttattc gtgggtaaga tggctacttt
     3061 tgcagaggca gcagatattg tttttttact tcctaaagaa aaagaaatgc aaacctattt
     3121 ggaacgagct tttcgctatt cttgcatttt tccgttgatc tttttgatat tggcatgcgg
     3181 attggccatg ccattagtgg tggttagtac ggagcaatcc ttttcgatgt ttttcgtcta
     3241 tctgatcata ttatggagtt tgaaattcag tcatctgcaa atcaagagag cagaattgtt
     3301 tcgactagat caacaagtta ttcgttattg gaaattaggc tggttttttt cttctctgat
     3361 ctttttgttt ctatcatttt attggctaat atgggcagga atagcaggtg cattagtaca
     3421 ggcaagtcta tattactggt ttttgtggaa gcaacaagaa aaaggattgg attgggagaa
     3481 aatggtgcat gtagaagaac ataggttgca tcagatctat caatttatca atttattcac
     3541 agatgttcct gaaattacgg caaagatcaa aagaagacgc tatttggatc ctatattgac
     3601 tcatattaaa agagaatcaa aaaacactta tctttacttg tttgcaagaa gattggcaag
     3661 aggttctgat tttagtggtc tttatttccg attagtaatt ataggtgggt tattgattgc
     3721 cggagtagtc gacttttatt ttagtctgat tatcggtttc ttatttttat atttgatcgg
     3781 tttccaactg ttacctttag cgaaccagtt ccgctatatg gttctcgttc agctttatcc
     3841 aatccataag aatcagaaga agcaggcgat tcagtatcta ctcacatggg tattaattat
     3901 cgcgtcggtt atttttggtt tgattgctgc agtcgtatta gatgggcagc agcgatgggt
     3961 tccgctagcg gcttatttga ttgttactat gctctttgtt aaactatatg taccaactcg
     4021 tttgaagaag atgtcagact gaaaacaaag ctgtgtaggg taaaaatgag aatgaaaaat
     4081 agagcgttga cagtaagaat gctgttcgtt ctattttttt tgagaaaagc tggttttttg
     4141 aatggcaatt aaatgaaaaa gaacgtatat gtgcttgacg tgtagtgttc aggcgactag
     4201 aataataagt actgcaaaac aaagagtaag aggacgggag aatgcatgat ggagtttcag
     4261 ttagaccaag agtggcggtt acgtcccatc aaaggcgaca caggaaaaac atacataggc
     4321 atgcgtgatg aggacaaagt ctttattaaa cgaaacacga ctccgatgtt ggctgctctt
     4381 tcaagggaag gaattacacc aaaacttgtt tggacaaaac gaacggggaa tggcgatact
     4441 ttgacagcac aagagtggct ggaaggtcgc ttgctgactc ctaatgagat tggtcaaaga
     4501 aacgacgtga ttgatgtttt gtaccattta catcattcaa attctcttaa aaccatgctg
     4561 acccgtatcg gtggtcgtgt catgacgccg gagaagatgc tgcaaaaata tgaaaaagaa
     4621 ttaccggaag aactccagaa aaatcaattt ctgcaaattg tccatcgttt tcttcgtgag
     4681 cagatcccgt caatagataa ggaaaactta tcagtagtac acggagatgt aaaccatcgt
     4741 aattggttag tatgtaaaaa ttatttatat ctagtcgatt gggattcaat catgtttgca
     4801 gaccctgcgg tggatattgc aacgatttta ggaaattatg tgccactgtc tagctggaat
     4861 cagtggctga tcagttatgg catacgcccg actgacgaaa tgcttgaaaa atttcattgg
     4921 tatgcggcca tgaatttgct ccaagaaatc accagatatt gcaaacgtga agatcatcga
     4981 cggatgaacg aagaaatttt acaattaaaa cgaattttca gtggctgagg tttggcccaa
     5041 actctgcgga gatttggcca gaccttttta attagaggaa aggaatggag ataaataatg
     5101 cgtgtccgaa atcgtccggg agcagcggaa atgttagcgg ctcacccgaa ttttgtaatt
     5161 agtgatccaa cattgtggaa gggaaaatgg aatgaattgt ttgaaaacga tcatccaatc
     5221 cacattgaga ttggtatggg gaaaggccag ttcatcactg gaatggcaaa agcccatcct
     5281 gaaatcaatt atattggagt agaaatgcag gtcagtgttg tttcgattgc actagacaag
     5341 ttgatcgaac aaccgcttcc taacctgaaa cttctacatg tggatggatc agcgttgaca
     5401 gagtatttcg ctgacagcga agtagatcaa atctatctga atttttccga tccatggcca
     5461 aaaaaacgtc atgaaaaacg gcgattgaca tataagactt ttcttgctgt agatgaacag
     5521 attcttcgtc caaatggaga aatccatttc aaaacagata atcaaggatt gtttgagtat
     5581 tctctggcaa gtttttctca atatggcatg atcctcaaac aggtatggtt agatttacat
     5641 caaagtcaat ttgaaggcaa tattatgacg gagtatgaag aaaaattttc ttcaaaaggt
     5701 caacgtattt atcgagtaga agctcaattt caagataaga ccaaaaagaa aagtctgtga
     5761 aactaacttt ctgctttcat aaaaacatta atatccataa ataaatccca gaaaatgaac
     5821 attacagatg taatgtattt tctgggattt atttgattta aaatgtattt atttcatcgg
     5881 tttttccaca aggtggaatc aattggaagc gagaaaatga aaataatcct ttgtcggtga
     5941 tcttcaattc gggtatgaca gataatgtta agaaagaaag tgtcaaaaaa ggatcaaagg
     6001 ttttagctgc tcctagttga tgtgctttag ttattaattc attcaactgt tcatagacga
     6061 tcgaaaaagg ttcctgtgtc atgattccgc ctataggaag agaaacacaa gctatttcct
     6121 gctgattgtt gacaacggtc atcccgccac ctttttgaat caaaagatta gcagcataca
     6181 gcatatcctc gtcgtttgtc ccaacgacaa ctagattatg cgagtcatgg gcaatcgttg
     6241 tcgctatcgc tccttgtttc agctgaaatc ctttcactat gcctaatcca atgtttcccg
     6301 tttgatggtg acgctcgatc accgcaatct ttaattggtc tttttcgatt gaaggaatga
     6361 atcgttcgcc ttgtcggtcc actttttcta ccaaatcttt agtcactaga ctattcggga
     6421 taatttcgat cacatgggct tgtgaagaag aaagaagcag ctctaacgaa tgtggattta
     6481 atggttgagc gtttagtttt ggcagacaac acgtatatga atttgaatga tcagcaaata
     6541 aatggtcttg gagttgccca gattgaacaa ctaatgttcc cttatagaaa acttcatgaa
     6601 ttggcagctt ggtaagatcg tctgttaaga agaaatcagc ttgataccca ggggcaatcg
     6661 cacctagtgt tttcaaatca aaacattctg ctgcattcaa tgttgccatc tgaatggctt
     6721 gtaatggatc gattccaagt gcaatagctt gacggacgat atgatcgatt gatccttctt
     6781 ttactaaatc attgatcagt ttatcatcgg tgacaaagaa gcatcttctt gaattttcag
     6841 gtgtgatcgc cggtagtagc gatgccagat cttttgcaac ggtcccttct cgaaccatta
     6901 aatacatacc aagatccaaa cgttctttag cttcttttgc agttgtagct tcatgatccg
     6961 tccgaatacc tgctgcagga taaacattta actcttccat gccgatacca gctgcatgcc
     7021 cgtcaatttt tttcttatgc tggtgcatca attggatctt ttctaggatg tctgtttcat
     7081 tatttgctac tgcttgatag ttcatgactt cagctaagcc taaaacttcg ggtttttgta
     7141 aaaaaggggc taattttttt gcatttaaat ttgcaccatt ggtctcaaaa ggggttacag
     7201 gtacacaaga aggtagcata aaaaagatat tcatgagtgt ttgtcttgca tcttctatca
     7261 taaactcaag acctgtactc ccagcaatgt ttcctatttc atggggatct gtcactacag
     7321 tagtaactcc atgaagcaag gatacttttg aaaattcgct tggtgataaa agtgagcttt
     7381 cgatatgcac atggccatcg atgaatcctg gcactagata ttgcccatta gcattatgta
     7441 cgctttttcc ttcgtattca ccgattccta cgatcattcc ttcgcagatt gctacatctt
     7501 tttccatgat ttctttcgta aagacgttga cgatcttagc atgcttgatc actaggtcag
     7561 caggcttttt tttacttgct acttcgatat atttccttaa tgttgtttcc aatggagtgc
     7621 ctcccaagat aaatttttat aagtataaca taaattcggt ttttgccaat atttttttgt
     7681 aaattcattt tatttgaaac aaaaagatca aaaagacgta tgttttttgt ggaggggaga
     7741 ataatggaaa taaaacgatt atcggatcaa atgataagtg aaagttttgc caaacatgcc
     7801 caagacgtat atatattgcc agcttcagaa gtattttaca tctattttcg ctatggaaaa
     7861 aaacgaacat tctggaagaa agtctcttcg gcagatggac agcaactgat ctcaagattt
     7921 aagtatttgg gacagatgga tatcggagaa aaaagaaaag ctcagttagg tgcatttact
     7981 tattctttag aaaaacagga agttcgtctg cggttgtctt ctgtaggaga ttaccttcaa
     8041 cgagagagtt tagtgattcg gctattatac ggtacttttg aaacacccca tcgttgcttt
     8101 agttctcgag attttgcaac tattttacag aaaaccgtca gacgtggatt ataccttttc
     8161 agcggaccag tcggatcagg caagacttct ctaatgtatc ggttggcttt ggaagaaaaa
     8221 ttgcaagtga ttactatcga agatcctgta gagatagaag aaccgttttt tctgcagcta
     8281 cagatcaatc cgaaaatagc acagacttat gatacacttt tgagactcgc attacggcat
     8341 cgtccagact tgctaatcat tggcgagata agagatgaat caactgcaca tgctgcggta
     8401 agagcagcac taacagggca ccgggttttt gcaaccgtac atgcacgcaa tcttgaaggt
     8461 acattggtca gaatccatga actgacaaag aaaaaagaag agctatctga atgtttagct
     8521 ggtgtcattt atcaagaact tttggaagat gccaatcacc atccttctgt tttatggagt
     8581 tatcaattta tggaggaaga atcagtaaaa aaatcatggg aggaaagtta tgaagaagct
     8641 caggaaaata tttggcagtc agaactattc taaaggatta acaagaagcc agcaaaacca
     8701 gtttgcacaa accatgggag aactgctttt atctggtttt agtctgcaag aatcccttta
     8761 tttattactc acgatccgtt cgtttccacc tgttattttg gcaaatatcc aacatcagct
     8821 aaaggaaggg caagcgttta gtgaggtact ttcgcaaata ggatatggag cagagcaact
     8881 ggtacagatc gagttagctg aacgtcatgg gaatctgccg acaactttgt tggaaattgc
     8941 caaacagttt cggcttgttg aaggatttcg caatgatttg aaaaagctgc ttgcatatcc
     9001 gatgctattg ctcacctttt tgctaggtat cttgtttagt atgcggttgt tcgttttgcc
     9061 tcagcttatt ggttcaggaa tggtcatgca agagcattgg ggtattcagc tgatacaagt
     9121 ctttcattgg tatgttctac tattcatcct agcggttact ctattggctt tactagtaag
     9181 gtatcgtctg atgaagttgt ctcctgttga tcagagtgaa tggataagca gacaattatt
     9241 gattggaccg atatattcct tgtatcaatc ggcttatttg tcactagaga ttggaaagct
     9301 ttttcatgaa ggtttcgaat taaggcaaat catagtctgc ttaaaggaaa cgaaacaaga
     9361 aagtctgatt cagcatcttg cctctaagtt gattcaagga ttggaggcag gtatgtcatt
     9421 ggctgagcag ttccagaatt atcgttttct atcagaagaa ttcagtaaaa ttgttttaca
     9481 gggagaggca aagggaagtt taggtaaaga actgattttt tacagcgaat ggacaagaaa
     9541 acagcttttc aaacgtcttc atcactggat gcattttatt cagccaatca tttttttggg
     9601 cgtagcggct ttgattttat tagtttatgc cgctgtttta ttacctgttt acggaagcat
     9661 agaggaggtt ttgccatgaa aaagagaaaa atatcttatt atagcggttt tacattgatc
     9721 gaaatgctaa tcgtcttact gatcatcagt gtattagtgt tgttgttcgt ccctaatttg
     9781 tcgcgttata ggaatcatgt agaccaagaa agtcgtgaag cgatcatcca gttggtagac
     9841 acacaaaaag agctctatgc gcttcaaaat aatggaagag ttcctactgt ggaggaattg
     9901 ttgaatgaag gctatatcaa acgagaacat gcagaaatct atcagcggcc ttgaaggtaa
     9961 ggttttagca tcaggatata cgttgtttga atcactctta ctgattttat tacttacaac
    10021 gatcctttct tttccagttc tcgcattttc tgcatggaag aatgaattgg cagtctatca
    10081 atttttcggc caatttgaaa aacgaatcta ttctacgcaa aaaatcgcta ttgtgaatca
    10141 gatccaaaca ggcttttatt ggaatagtga agagaatcaa atcatttttc atgttccgaa
    10201 tccagataaa ataaattggg aagtattaga tattcctgat gagattacct tgaaacgcca
    10261 tgcttctatt acttttgctg ctagaaccgg gaatgaaagt tcactaaaag cttatcaatt
    10321 ttattgggaa gaaaaagagc ggacgattac gtatcaattc cagataggga gtggtcgcta
    10381 tacaaaaaga attgaataaa acaggatttg ttttgttgga gtcccttaca gcgcttatga
    10441 tcgcttctgt ctgtgtctac ttagtgagct ttggccagct gtttttgctt caggcagaaa
    10501 agaaaagtgg ggaggaactg ctatttattc gaatgattta tgaagatatc aagacgcagc
    10561 ggatgtatca caaaagacca gagcgaaggg aagaaatgat cccatacgat atccaaattg
    10621 tcgataataa tggagtaatc gaaaaagtgt ttgtcaatat ggagaaagaa gcgatcagta
    10681 ttgaaaaaac aagtaattcc agcatttacg ctgattgaat gtctaattgg actgatgata
    10741 ttgagcagtc tattgctcac attcaatctt ttgattcagc aaaccatcca agtaagtgaa
    10801 tttttgcagc gtaaagatca aaaagagtgg ctaatatttt tagcacaatt agaagaagaa
    10861 ctcaagagca gtcacaatgt ctctgtgaaa aatcagcaac tgcactacac gatcgaggat
    10921 aaacagtata tcatcgaaag atatcaggca atgatacgta aaagaagaac ttccggcgga
    10981 catcaaccta tgttaacaag tatcactgag cttcaattat tggaaaaaga acacacaatt
    11041 tctttttatg tacactttga aaatggagaa gatggatatg ccatatggac aaaaaacgat
    11101 cagtagaaaa tgttataagg gctctatttt attttctgtc ttgcttttat ttcttttatg
    11161 tacgttttta ttgatggcag cattagaaag ctaccgtttg tcaatggatc ttaatatacg
    11221 aatcaaaaat tattatatag caaaaataat gaagcagatg gctttatcgg aacagtctac
    11281 gcttgccgaa agttcggaag gagtttttta ctacacaact ggatctgtaa catatcagtg
    11341 ggtacaacag tccctattct tagaggtgga agtctcaccg tttatctttc ggttcacaga
    11401 agaagtaaaa aatgatacag atactagtac agaatgatgg aatgttcgtg aaaacgccct
    11461 cttttctgtt ataacactgt ttttaataaa aaaatgtttg ccgtttcaca aataaaaaac
    11521 agtgaaaacg attgaaattt tgtcaattgt tcgatagaat taaacaggtg aaaaaattgt
    11581 tataatatat ctagaaatga ggtgcaccat gttaccagaa aaaatggaac aagggtttaa
    11641 tcaaaacctg gaagctattc agcttttaca gaatgcattg ggaacttcct ttttagaagc
    11701 ctatgtcgaa aatgcagaaa atttaataga tgattatcaa gtacgtgtgg tggatggagt
    11761 gccgacaaaa gagacaacgc aacggatcac agctctttat gaagagttga agaaactttc
    11821 tttcgagcca gaagaatggc gcagactgtc tcaattactt ttattgaagg gcagccagac
    11881 agaacatttg caggcgaacc atcaactcac tcctgatagt atcggttttt tatttgtttt
    11941 tcttattgaa caactataca caaataaaaa agcaccggtt aagattctgg atattgcggc
    12001 aggaatgggc aacttgttat taacagttct tttaaaccta tcgaatgcgg ggtaccaaac
    12061 agaaggaatt ggtgtggata ttgatgatac attgttggct gtcgcagctt ctacaagtga
    12121 tttgacacaa gcaaatgttc aatactttca tcaagacggg ttgcaggaat tattaataga
    12181 tcctgtcgat tttgcaatca gcgatttgcc aatcggttat tatccgaatg acgaaaaagc
    12241 aaaagagttt ttaacaagta ccgaagaagg ccatagttat gcacatcatt tattgctaga
    12301 gcaatcaatg aaatatgtga agccggatgg ttttggtttg tttttgatgc cgagtggatt
    12361 tttagaaacg gatcaaagtg aagaaatcaa aaaatggttt aaagaagaag gttatcttca
    12421 aggaatgatt caattgccag atgaattgtt ccgtaataaa caatcgcaaa aaagtatttt
    12481 gatcttacag aaaaaaggac cacaagcaca acaagtaaaa gaagtcttgc ttgtgaaact
    12541 tgcttcatta aaagaaccag agaaggtgac ggaatttttc aacgagttta aaaactggaa
    12601 atcatctgct ctgtaatcat tcaacgagaa ggagagtcat atgtctaaaa caattgcaat
    12661 caatgctggg agttcaagtt tgaaatggca attatatcaa atgccagaag aaaccgtagt
    12721 tgcgaaaggg atcgtcgaac gtatcggttt acaagattct atttttacca tcaagtacgg
    12781 cgataatcaa aaattcgaac aaatcatgga tatcgaaaac cacgaagtag cagtaaaaat
    12841 gctgttagat cagttgattg aactgcaaat tttaagctct tatgatgaaa tcaccggagt
    12901 agggcaccgt gttgttcacg gaggagaaac ttatggggat tcagtagtga tcgatgagaa
    12961 agtaatggac cgcattgctg aattagcaga atttgctcct ttgcataatc ctgcaaactt
    13021 aatggggatc aaagcgttta aaaagatttt acctgacatt ttgagtgtgg ctgtattcga
    13081 tacatctttc cactcaacaa tgccaaagca aaattattta tatagcttac cggtgtctta
    13141 ttatgaagat ttcaaagtaa gaaaatatgg cttccatggc acaagtcatc gttatgtgtc
    13201 ggaacgtgcg gcaaaattat tgaacaaacc gatcgaagaa ctaaaaatca tcacttgcca
    13261 cttaggaaat ggtgtatcga tcacagcagt cgatggaggt aaatccatgg atacttctat
    13321 gggattcaca ccattagctg gggtgacaat gggtacacgt tcaggtgata tcgatccagc
    13381 tgttctccca tatttaatgg aaaaattaga gattgatatt gatgaaatga tcaatgtttt
    13441 gaacaagaaa tctggtctgc taggcctaac aggattgtct agtgatatgc gtgatcttga
    13501 aaagaactat gaaaaagaac atattcgttt agcttatgac attttcgtgg accgcatccg
    13561 aaaatacatc ggcggttatg tgacaacgat gaatggtgtt gatgcaattg tctttactgc
    13621 tggtatcggc gagaatgacg gacatgtacg tagtgaaatc atcaaaggta tgtcttggtt
    13681 tggttgtgaa atcgatccag cgttgaatga attgcgtggt gaagaagtag atatctctac
    13741 aaaagaatca aaagtaagag tattagtgat cccaacagat gaggaactaa tgattgcccg
    13801 tgatgtggag cgtctgcgcg gctaagccaa agcattcgtt aggataaaag atgaaacgca
    13861 tgcaagaggg aattcttgca tgtgtttttt ttgcggagaa gaccgcaaaa tggtaattta
    13921 acttcaattt cccttctttt ttacaggatc ttcttaggga attttgcttg gaaaagtggt
    13981 caaaagggaa aagactggca ttttatttat tgattttcag gaaaatccca atattttatt
    14041 ttcattcatc tttataaggg acattacata tgtaaaaaga aaaaacattt ttagatcagc
    14101 ctcgtgggct gtcgacttta ttcttcgctt tatgtttagc tagaaaaccg atccgaaaca
    14161 ttcattgatc gaataataaa aacttgattt tccacctgta ctttgcctga atcgtgaaag
    14221 cacaggtatg aaaatcaagt tttttgttgc gaaaaaacgt cctagtcaat cagttggccg
    14281 agcggctggc ggctgatctg ctcctgatca ataacgagtg cttcttggaa gtgttgctgc
    14341 aaaaagtagc ggtcatggct gactgctagg agcgttcctt gatagtcttg cagcatttct
    14401 tcaatttctt ctcgagcata gctgtcgagg tgattggttg gttcatccaa aatcaggacg
    14461 ttgatttttt ctaaaaagag tttcagcaac gccaaacgga ttttttcacc accggataaa
    14521 tctttgatcc gtttacccac atcgctttga taaaagccac ttttggctaa ttgccgtcga
    14581 gcgacttctt cgtttccagt caatgtgatg gcgtaagcca gcaaacggct atcgggatcg
    14641 gcaaactgca gctgctgcgg caaataaccg atttgcaagc tactacctaa tttgatggtg
    14701 ccaccgtaag gcttttcctc accgagtaat aatttgagta aggttgattt tcctgcacca
    14761 ttgtcgccga tgatggctaa atgatctcca cgatagactg caaaactgct gtctttgaat
    14821 agtacttgat cagcgtactg ctggtgcaag ttttgaatga tgaaaacttc tttgccagat
    14881 tttccaccat tagacaagct ttgcagccga tttttcgggg gaggtggcgg tttgacgagt
    14941 gtcagtttcg ccaggcggcg ttccagttct ttggcttttt tgaagaagga ctcattgtct
    15001 ccttcatgtg cccactgacg aaaacgacga atcatgactt tcaaccgctg gacttctttt
    15061 tgctgcagtt cgtaattttt tgtcagctca gccaagcgag cttctttcaa gactcgaaaa
    15121 cggctgtagt ttccgggata ctcaatcaat gcaccttctt ccagttccca tgtttttgtg
    15181 gtgacttgat cgagaaattg ccgatcatga gaaacgacca gaaccgattt ttttgtattc
    15241 tgtaaatagt tttccaacca gcgaatactg ttaagatcta aatgattggt cggttcatcg
    15301 agtaaaagta aatcggcatc ggctgctaaa attttggcta aagcgacttt aactcgttcg
    15361 cctccgctaa gttctgttaa ccgtgcttcc tgacggtgtg gaatccctaa gccttgcaag
    15421 atcgctgcaa tccggtcttc taatacgtaa ccgcctagtt cttcaaagcg atgctgcagc
    15481 tcaccgtaac gggccagaca cttctctaag tttgtatctg gttctgtcat caatgcttcg
    15541 tagcgactca tttgctgttt gaggtcctgt atctcagcgg cagaatggag caagtagtgg
    15601 ttgacggtcg cttcggaaac tgtaagttct tgttcgaccg tcccgatctt taagcctttt
    15661 ttgcgactga taacaccgct gtcgacacct tctctgccgg tcaagatttg taaaaaggtt
    15721 gatttgccag ttccgttgat accgactaag acaatttttt caccagcttc aatttgacag
    15781 gataaatcct caaataatgg attcccgccg attgtttttt taatagaaat tgcttgtatg
    15841 atcatgatct ctctccttga gaccactcaa aaaggccatg gttatccatg gcctgtgtta
    15901 tttaaactca gattatcatg agaaaaatac cttgagatgg ccattgattt tacgtgaatt
    15961 caactgtttt ggacaaactt gtcccgtcaa cagcaactgt aaaaccaatt gcatggctaa
    16021 atgcgatatg gtatcgttgg taattttcat tacttgttat tttagtcatg cgcatcatag
    16081 tattttccct ccttcgtgta gttgtctttt ccagtgtacc tgaaagtgtc agaaagttca
    16141 actagcgctg acatttactt aatatttatc gatgtgtaat tctgtcagag agcttttttt
    16201 atctgactga gggtaatgtc tcgtggacca catcattgta ttctgagcta aaccagcgtt
    16261 caggcgaaat catatggtgt ttgataaaaa actttgtcta catgacttcc ttttgccatt
    16321 tgcttaatac cgttgcatta aacagctctt ctctttggaa atctcaccaa catcgcgttc
    16381 aggagtatcg ttgtggttgc aatggcaagc ggtagccttt catcaattct tgaatgattt
    16441 accgaatgta gcacttgcct tctcgcggta cccgaacccg aaataaaatt ttagtttggc
    16501 gataaaatcg gtatccgcca tcgatacttc atattttttc gtatcagatt catcggaaac
    16561 ttccacattg acgaaccaat aagctttggc atgcttaggt cgttttaaac cacaccaggg
    16621 cgactaatcg ccctggtgtt cacttttttt tattttactt gaactcattg actgttttcc
    16681 ctttatttta aacattataa agaccgatga gtgcttgccg cgtcttttgt ttttatcacg
    16741 gttttttgtg caaaaaaacc tgccgatagt tggcaggtcg tctacgtgtt aagctttggt
    16801 gtggtcttta gctggcaatt ggttttctaa atcagttcgg acaacaagca catcgcaagc
    16861 tgcgtttcta ataacatatt cagaaacaga accaataaat aaacgttcta cagcattcaa
    16921 tcctgtggct cctaacataa tcaaatctac ttgattatct tctgggattt ctctggcaat
    16981 aatttgtttt ggtgagccat attctacgac agaggttaca ttattcaagc cagctttctt
    17041 cgcattgctt tcataatctg ccaatgtttg ctttgccatt tcagtagctt gttctgcaag
    17101 cattccatca aaggatgaaa cggtttggaa agaacgtgtg tcaatgacat gtgctaatag
    17161 aagttctccg ttatttcgga tagctacatt gaccgccttt ttaaaagcta attcagcttc
    17221 atcagaacca tctacggcaa ccatgatttt tttgtattgt tgtaacataa gcattcactc
    17281 ctcaagtcaa tcgtattgta ctcttttatt gtagaacact ttagggaaaa agagaattat
    17341 ttactgcttt ggttcagcaa ttttttttgc aaatcggata ctgaaggtgg cagaaacaat
    17401 aattacgatc agtaaaaaga ccaaggcaat aaagcgttga ttgaaaaaag ctgttaaaat
    17461 acccattacg cctaaaacag catatgcagc tccgtaaaat tgtaggaatc cttgattttt
    17521 atcatttttg ttgatcaata caaaaaagat atcctgttta ttaaataaat aatagctaac
    17581 gaataataat actaaagtgg cgataatcat taaaatccta atcatgaaca aatcctccaa
    17641 aataagcaac aaatagaaaa agcaatttgc caagagcaaa ttgctttatt ctttaaaaga
    17701 tggtaatctc cgttaatgcc gttgtttgtc ctgtagtatt gatcccgcga attcaagcag
    17761 gtgggtccca tggtcgtagc ttcgaactac caaatgataa ggcatgttac cagcagtgac
    17821 acaagacggg tcccaagata tcaataaaaa tgttcggtca acacacaaag aggacaattc
    17881 gaacatcaca gatttcccac ctctatagta gcatatgaat ttaaaaagag caatgagaga
    17941 gctcgcagaa acagaaccgt tttcttgtct agttttttgg aggatgcttt ttccactctt
    18001 ttatacggta atactgttga cccagagcct gttcatattt ccctgtatct tttggctgat
    18061 aatactgagc attcttcagt ttatctggta aatattgctg attgacccaa gcctgatcaa
    18121 aatgatgagg atactgataa cccacgccac ggttgagtga tttcgcacct ttgtaatggc
    18181 tatctctcaa atgatcagga acatctccag ctttgccctc tcgaatatca gcaagtgctg
    18241 catctaatgc catgtaggca gaattggatt ttggcgacaa gcaaagatcg acgacgacgt
    18301 ctgctagtgg gatacgtgcc tcaggcaatc ccaatttttc agcagctaat acagcgttca
    18361 ctgtacgagc cgcagcagca ggattaccta aacctatatc ttcataaccg ataaccatca
    18421 gacgccggca aatacttgcc agatcacctg cctcaactaa acgggcgaga taatgaagtg
    18481 cagcatccac atcacttcca cgaatcgatt tttgaaaagc cgaaataacg tcataatgag
    18541 catctccatt tttgtcatgg gtaagagctt ttctttggac acattcttca atgatcgaca
    18601 gtgtaagatg aatccttcct tctttatctg aaggagtcga tagtgtagcc agttccagcc
    18661 cattcaatgc actccgcaaa tcgccgttcg ttgcacgcga aaggtgaaga agagcatctt
    18721 cgtctaactg gattgcttgt tgtccaaggc cgcgttcttt atcccttaaa gcatgttcga
    18781 ctgccaactg gatgtcttgt tctgttaatg gtttgacttc aaagatctgt gtacggctgc
    18841 ggatcgctgg attgatggta atataaggat tctcagtagt cgctccgatc aagatgattc
    18901 ggccactttc taaatgagga agcagaaaat cctgttttgt tttatctaat cggtgtactt
    18961 catccaataa gaggatcact gtaccactca tttttgcttc ttccgctaca acttgtagat
    19021 ccttttttga atctgtagcg gcgtttagca tgcgaaatgc ataattagta gagccggcga
    19081 tcgcacttgc gatactcgtt tttccagtac caggaggtcc atatagaatc atggaagaga
    19141 gcatgcgagc atcgaccatg cggcgtatga tttttccttc cccgactaga tgctgctgac
    19201 caacgacttc atcgatcgtt cgaggtctca tgcgataagc caatggctgt tgcataggat
    19261 tttcctcctt cttgttttta cgtcccttaa ttatatcata tttagcaaga tgcctagaaa
    19321 gaacagcctt ctgttgtttt gttttcatag cttatccaac taatattgtg tatgataaag
    19381 taggtgagaa aaatgacgga taaacaaaag atagatgaat ttttttggaa taaaacgacg
    19441 ccagaagtgg cacgggattt attggggatg tatttggagc atgacacacc agaaggaaga
    19501 ttagctggat atattgtaga cgcggaagca tacctaggtc cagaagacga ggccgcccat
    19561 agctatggtt taagaagaac accgcgtgtg cgggcaatgt atgaaaaacc aggtacgatc
    19621 tacctttaca cgatgcatac tcatcggatt ttgaacatca ttactcagcc agaagggatt
    19681 cctcaaggag taatgatacg tgcaatcgaa cccgccgcaa tgattgatca aatgagtaaa
    19741 aatcgtggcg gaaaaactgg tccagatatc agcaacggtc cgggaaaatt agtggaagca
    19801 ttagcaattc ctcaagaact ttatggtcag tctatagcag atagttcgtt acgcttagtt
    19861 tttgagaaaa aaaagacacc aaaaaagatc aatgctttgc ctagaatcgg tatcccaaac
    19921 aaaggtgtat ggacagaaaa gccgttgcgt tttgttgtgt cgggaaatcc ttatatctct
    19981 ttacaaagaa aaaaccaaat cgagaaagat tggggttgga ggaaagaaaa tgaaaaagaa
    20041 ggatctatta acatatttag atgaaataat tgaaaaacaa gtgacagact atgatgtagc
    20101 tttagattgg gatacaaaaa atcatacgat cgagatcgtt gttcgcttgt ttgcagaaaa
    20161 taaagcacaa tttgagattg atgatgcaga agggatcgtt tcgcaagaac caattatcga
    20221 atttgaagac ggcgtacttc tttttaatcc tcagaaatct gtttttgatg aacgcaatta
    20281 tctagcagta atcccttatg aaggaaaaaa agggttggca aaatcagtag ctgattcttt
    20341 agtggaatat ctaaatgaag tattagctca agggcaaagc gatttgcttg attttttgga
    20401 tgaagacgat gacgaagctg tattcgagct tcactgggat aatcagcaac tagcagaatt
    20461 agtagaagta aaacagcaaa atgaagcaga tacttattta ccgtacccaa gttattaagg
    20521 agtgacagat atgaaatgga atgaagtaaa agtagaaact gaaagtgaag cagtcgaagc
    20581 agttgctaat attttgatgg aggcaggggc gagtggtgtc gcgattgaag attctttaga
    20641 tgttgaacat tttcagtcgg atcaatatgg tgagatttta gataaaaatg attttacatc
    20701 gctgaaagaa ggcgcactag tcatggctta ttttccggaa acgactttct taccggaaat
    20761 cttgcctttt atcaaagatc gtatcacaca attacctgag tttggtctag ctattggaaa
    20821 aaataaagta acagttagtg aagtagagga aagcaactgg gcaacagcct ggaagaaata
    20881 ctatcatccg gtgcgtatta cgcgaatgct gacggttgcg ccaagctggg aaaactacca
    20941 gacagaagat cctctagaaa aaattatcac actagatcct ggtatggcat ttggcacagg
    21001 cactcatccc acgacttcct tgacattgac agcactagaa acagtcttgc gtggtggtga
    21061 aacattgcta gatgtgggaa ctgggtcggg cgtgctaagt attgcagcaa aatatcttgg
    21121 tgcaaaagat gtctatgcct ttgatttaga cgaagtagcc gttcgttcgg cgaaagaaaa
    21181 tatggatatg aatgaaatag ccaaagatgt ccatgtatca gctaacgatc ttttgacagg
    21241 aatcgagatt gaaagtgatg taatcgttgc gaacatttta gcagatatca tcttattgat
    21301 gatcccagat gcttggcgtt tactgaagca aacaggcacg ctgatcgttt caggaatcat
    21361 tgaagagaag aagcaaatgg ttctggatgc catgttagaa caagggttta tcgtggatca
    21421 aattttccag caaaaagact ggtatgcaat tattttgaaa aaaccagagg tggaatagat
    21481 gcagcggtat tttttaaaag aatcatatga atcaaaagac tattttgttt tagaagggga
    21541 aaactatcac cacattgtac gggttatgag gatggaaccc gaggatgaag tttttttggc
    21601 attcaatgat caaatagcca ttgttgcaga aatcacggag attactgaag aggctgttta
    21661 tctgaaagaa attaaaaaag aagaaaatca aaaggaactg ccaagttcga ttaccatagc
    21721 gagtggctat cctaaagggg aaaagctgga ttggatcgtt cagaaatcca ctgaattagg
    21781 agcgcaagct tttcttggtt tcccggcatc agcctctgtc gttaaatggg atgcaaaaaa
    21841 acgtatgaaa cgacaacaac ggcttgaaaa gattgctcaa gaggcagcgg aacaatccca
    21901 tcggcagatc gtgccatcag tagagttatt ggaaacgaaa caagatttat ttgagcggct
    21961 agatgattat gacgtggtct tggttgctta tgaggaatca gcaaagcaag gcgaaaaatc
    22021 aaatgcagcc aaaattctta gtgaaacaaa accagatagt cgaatcttag ctgtctttgg
    22081 accagaaggt ggattatccc gcgatgaagt cgattatttt gtagaacaca aagggatact
    22141 ttgtggatta ggaccaagaa tccttcgtac agaaacagca cctctttatt tattaagtgc
    22201 tgctagctat caatgggaat taatgaatcc ttctttcagc taacgttctt cattaataaa
    22261 agagtatcga cagaaagaat tgaaaaaata gtcaacaatt tgtataatag aaacaattat
    22321 ttatacaatg gatacgaaaa atcgtttgaa aaagctttta aaaagcgaac atttccgtaa
    22381 aaaaacgtat aactgcacgg attttgggac aattatcaaa cagatatttg tcccgattcc
    22441 cgttttaccg aaaaaagaaa gggggatcga ttcctgtgac aaaagaagaa attttaacgg
    22501 gacccggtgt cataaagctc gttagtcaat acatgggtcc cgaacatgta gcatttgttg
    22561 agaaagcatg cgaatacgcg actgctgcac atgacggaca gtttagaaaa tcaggcgaac
    22621 cctacattat tcatcctatc caagtcgcag gtatattagc agatttaaaa atggatcccc
    22681 atacagtggc tacaggcttc ttacatgatg ttgttgaaga tacagaaatc actttagaag
    22741 atctgagaga agaatttggc gatgacgttg ctatgttagt ggacggcgta accaaattag
    22801 ggaaaataaa atataaatcc cacgaagagc agctagcaga aaaccaccga aagatgctgc
    22861 tagcaatggc tcaagattta cgagttatca tggtcaaatt agctgataga ctacataaca
    22921 tgcgtacgtt gaagcacctg cgagaagata agcaaagaag gatcgctcag gaaactttag
    22981 aaatctatgc gcctcttgca catcgtctag ggatcagccg gatcaaatgg gaattagaag
    23041 atacagcact tcgttatcta aatccaaaac agtattaccg tatcgtccac ttgatgcaga
    23101 cgaagagaga agaacgtgaa aaatacgtaa gtggtactgt tgaagatatt cgaatagcga
    23161 cagaagagct ggggattttt gcagaaatct atggacggcc aaaacacatt tattcaatct
    23221 atcgtaaaat gaaagatcag aaaaagcagt tcaacgaaat ttatgacttg ctagctattc
    23281 gagtgatcgt agattcaatc aaggattgtt atgctgtatt aggagcaatc cacacaaaat
    23341 ggaaaccaat gcccggcaga tttaaggatt atattgccat gccaaaagct aatatgtacc
    23401 aatctttgca tactaccgtg atcggtccgg caggaaatcc ggtagaaatt caaatccgaa
    23461 cacaggaaat gcatgaaatc gctgaattcg gggttgctgc acactgggcc tataaagaag
    23521 gaaaaaatga aaaagtagaa ccagatggta tgacgaaaca attaagctgg ttccatgaga
    23581 tactcgaact tcaagacgaa agctatgatg cttctgaatt tatggaaggc gtaaaaggag
    23641 atatctttag tgataaagtc tacgtcttca caccaaaagg agacgttact gagttaccaa
    23701 aaggatccgg accattggac tttgcataca gtatccatac agatatcggt aacaaaacca
    23761 ctggtgcaaa agtaaatggc aaaatggtgc agcttgatta caaattgaaa aacggagata
    23821 tcattgagat catgacttct ccaaattcat ttggcccaag tcgcgactgg ttgaaattag
    23881 ttgctactag caaagcaaga aataagatca aacgtttctt caaagcccaa gatcgagaag
    23941 aaaatgtgat caaaggccac gaatccgtgg tcaaatgtat tacagatcta ggatttacgc
    24001 ctaaagatat tttgacgaag aacaaactgc aagaagcact cgatcgtttt aattatcaaa
    24061 cagaagatga tctctatgca gctgtagggt atggagaagt tagccccttg acgatggcca
    24121 atcgtctgac tgaaaaagaa cgtaaagaac aaaaaatcga gcagcaaaag caagaagcag
    24181 aagaaatcat gaatcagccg aaaaaagaac ctgacaaaat gaaagtacgt catgaaggtg
    24241 gcgttgtcat tcaaggggta gaaaacttac taattcgtat cagtcgctgc tgtaatccga
    24301 ttcctggtga tgatatcgtt ggttatatca ctaaaggcag agggatatcc attcatcgtc
    24361 gagattgtcc gaatgttcag cctgacaaac caaatgtagc agaacgtttg attgaagtcg
    24421 aatgggaaga tacatcgaat acacgaaaag agtatgatgc agatttggaa atttacggct
    24481 ataatcgttc aggcttattg aatgatgtac ttcaaacagt caatgcgcta acgaaaaatc
    24541 tcaacagcgt tgaagcacgg acgaataaag ataaaatggc gacgatccat ttgacggttg
    24601 gtatccagaa tttatcccac ctaaagagta tcgtggataa aatcaaagca gtacctgatg
    24661 tctacagtgt acgccggacg aatggatagg aggacagata tgagagcagt tgttcaacgg
    24721 gtaagtaaag ctgctgtgtc agtcgatcag caagaaattg gaaaaattgg ctacggccta
    24781 cttgttttat taggcattca tgaaaccgat acgcaaaaag atgtggacta tctggtaaaa
    24841 aaaattgcac aaatgcgtat ttttgaagat gaacaaggaa aaatgaatat tagtgtagaa
    24901 gatgtgggag gacaaatttt aagtatctct caattcacac tgtttgcgga tacgaaaaaa
    24961 ggaaatcgac caagctttgt ggaagccgca cgaccagaaa cagctattcc tttatacgaa
    25021 tcattcaatg aaggaatcag aagtcgcggc atcacagtcg ctaccggaca attcggagca
    25081 gatatgtcgg tttctctcgt caatgatgga cctgtaacga tcattattga ttctaaaaac
    25141 aaataaagta atctggtttt cactagtaaa aactatgaaa cacgtcgctt aagttagact
    25201 gataaactct aacttaagtg gcttttttgt ctgcaaaaag cgcttctttt gtcagcagtc
    25261 ggaaaaaaag ttaagcttaa attatacaag ttatttttta taggaggttg ctgaagatga
    25321 aagaagtacc agtagtagat atcaaaatca cagatttaaa aaaattgtat caaaaaggat
    25381 acaacatgtt ggaggagtta cgccatgaag cggacgctcc cgtcgtcaaa gcaaagattt
    25441 tcaataaaga agctatcacg atctacggat catcagcagc aaaagtattt tatgatccaa
    25501 ggaatttcaa acgaaaaggt gctatgccaa aattagtatt gaagacactt tttggtcaag
    25561 gtggagtaca gactttggat ggagctgctc atcaccatcg gaaaaatatt tttatggatc
    25621 taatgacacc ggaaagaatg gaagattatc atcgtatctt agataagaat ctgacacaag
    25681 cacttgaagc acagcatggt caatttgaac tatttgattt aagtaagatg gttttcttta
    25741 catctatttg cgagtgggca ggtatcaatt tatcagcgat ttctaaagat gaggtcgaaa
    25801 aattagcaga atatcaaatc tctatgatca gcggaacgtt tacttctccg attgatcaca
    25861 taaaaggtgt cgaaaatcgc aaaaaatcag aaaaatgggc gcaaggcttg attgaagaag
    25921 ctcgacaaaa tcctgtggct ggaaaagaaa atgtggcatt atatgctttt gccaatgcca
    25981 cagatttaga tggtcagctt ttacctctgg aagtggcagc tgttgaatta ttgaacatca
    26041 tccgtccaac ggttgctcta actgtgtggg cggcattgat gggacatgca cttttcagta
    26101 gaccagattt gtatcagcaa ttaaaaaatg acttttcgac gttacaagat ccgtttattc
    26161 aagaaatgag acgctattat cctttcttcc caatgctacc tgcgatttcg ctgaaagaag
    26221 tagaagtaga tggttatcgt ataccagaag gcagttgggt tattttagat ttatatggaa
    26281 cagaccatga tgaacgaaca gttgaagcgc cagattcctt tatgatcaaa cggtatgtag
    26341 ggaaagcaaa agatatttcc tataaagaag aatatgagat gattgcacaa ggcgggggaa
    26401 attttcgaca aatgcatcgc tgtgcaggag aatggatcac tcttcatagt ctacgtgtgt
    26461 tttccgatca gctagtaaat aagtttgagt tctctgtacc tgaacaagat tggacgattc
    26521 cattcaatca gtttccgact tatccgaata gccgagcgct actttacaag aattagatta
    26581 gcataaactt atcgaaaaaa aggaagatca aacagtgctg atcttccttt tttcatatta
    26641 ctataaattg ataaggtcaa ttgatcaaaa tccacgaaat gcttcatcaa tggtttgata
    26701 atcttcattg gataaatgca aatctaaagc ttggacattg tcagatactt gctctggacg
    26761 cttagctcca ggtataacta cagacactcg tggattcttc atgtaccaag ctaaaacaag
    26821 ttgcgcagga gtggcttggt atcttttagc aatggggcga agtacatcga cagcggttaa
    26881 aatttctctg aaacgttctc cttggaaatc tggatttctt tttcgaggat ctccttcacc
    26941 aaattgtttt tcctcaccaa gttcatattt tcctgtcagc aaaccggaag caagaggaaa
    27001 atagggaaca aaactgattt tgtttttttc aagataaggg aataattctt tttcagcctg
    27061 ccgatggatc aaactgtatt tatcttctac tacatcgaca tatccatctg cattcgcttc
    27121 tttcaattgt tctaacgtga agtttgacac accgactgct cggatttttc ctgcttcttt
    27181 taattcgtgc aatgtagcaa ctgattcatt ttttggtgtg ctttcatccg gaaagtggat
    27241 ataaaaaatg tcgatgtaat cagtttgcag acgttttagt gcatcttcca ccgattgttt
    27301 gagaaactca ggagaattat caaatgttcg ccctttattt ggtacatgag cagcttttgt
    27361 agcgatgacg acacgtgagc gatcatattc tttttccttc agtacctcgc caatcagttc
    27421 ttcggaacgg ccgtttccgt aagcataagc ggtatcgatc aaattgatcc cgctattcaa
    27481 agctgtacga actacttgtt ttcctgtctc atcttccaaa ccgtcaaaaa gattatgtcc
    27541 tccaacggca tttgctccta atccaagctg ttctgcgtag acttgtgaat gtccgattct
    27601 tatttctgcc atagctgatt cctccttttt gtctagtcta aaacaaaata ccaaaaagct
    27661 gaactaaaac gtttttgtga ggggaaatag tcagatatct cttgactttg agactggttt
    27721 ttagtaggtt ataagtaata tcgaatgtca atgaaagagc gagtaagttt ttgaaacttt
    27781 acaagagagg atcatcgagg ctgaaaatga tctaaagttt cattagccta aagacactct
    27841 ggagatggac gagtgaaaag ccgtcccgcg tacgagcgtt attcgtaaga ggaaagagaa
    27901 atctttcaaa attaggtggt accgtgtatg aatttcattc acccttgttc atgatgaaca
    27961 agggtgattt ttttatttac atagctaaga aagggagaga aaactatgag tttccaaaga
    28021 ccaaaaggaa caaatgatat tttgccaggc gaatcagaaa aatggcagtt tgtcgaagaa
    28081 acagcacgtt tattattcag agattatcag tataatgaaa tgcgtacacc gatttttgaa
    28141 cactatgaag tgatatcccg aagtgtaggg gatacaacgg atatcgtttc aaaagagatg
    28201 tatgattttt atgacaaagg ggaacgccat gtgacgcttc gaccagaagg tacagcacca
    28261 atcgttcgtt cttttgttga gcataaattg ttcggcccag agtatgcgaa accgttcaaa
    28321 actttttata tgggaccaat gtttcgctac gaacgccctc aaaaaggacg gttacgtcaa
    28381 ttccatcaaa tcggagtaga agcatttgga tcagaaaacc ctgcaactga tgtggagagc
    28441 atggtgatgg cacttgattt cttcaaacag ttggggatca cgcaaatccg gctagtcatc
    28501 aattcattag gtgataaaga aacgcgtcaa gcctatcgtc aagcattgat cgactatctt
    28561 ttaccttttg aatcagagct gagtgaagac tctaaacgcc gccttcatga aaatccatta
    28621 cgcgtattgg acagtaaaga caaacgagat caaaaatttg tggcagaagc tccatctatc
    28681 ttagattatc taagcgagcc tgctcaaaaa cattttgata ccgttgtttc catgctggat
    28741 gcattggctg ttccttatga aatcgacagt aatatggttc gcggactaga ttattacaca
    28801 catacgattt ttgaaatcat gagtgacgca ccaaaaatgg gggcacaagc aacgatttgc
    28861 gcaggcggac gttatgacaa tctagtggaa gaactaggtg gtccttcaac acctggcttc
    28921 ggatttgcaa tgggtatcga gcgattactc ttgactatgg aggcagagga agtagttatc
    28981 cctgcattta atgagttgga tgcttacgtg gtcgcactcg gagacgaaac aaatatcgaa
    29041 gcattgaaag tggtccaagc gattcgaaac tttggttttt cagctgaccg cgacttcatg
    29101 aaccgtaaag caaaagcgca atttaagaca gcagataaag cgaatgccaa attagtattg
    29161 gtgatcggtg aagatgagct tgctagtcaa acgattaatg tcaaagcaat ggcaagccga
    29221 aaagaaaaaa gttttccatt agaagagata tatgaacatt ttgatgaagt ctatgatgaa
    29281 atgactttat tcgagtagga ggaaaaaaat aaatggcaca aagaacgatc tattgcggat
    29341 tagtttccga acaacaatta gaacaaacag ttacattaaa aggatgggta caaaaaagac
    29401 gagaccttgg gggagtaatc tttatcgacc tacgtgatcg tgaaggaatc gttcaaatcg
    29461 tctttaatcc tgaaatcaac aaagaagcat gggaaatcgc ggataaatgt cgcagtgaat
    29521 acgtgatcga agtgactggg gaagttagaa gacgcggaga aaatgcagta aatcctaaaa
    29581 tggccactgg tgaattcgaa gtgattgcaa gtgaaatcgt gattttgaat caagcaaaaa
    29641 ccccgccatt tcaaattgaa gatgatcaag caatcagtga cgaaatacgt ttgaaatatc
    29701 gttatttgga tctgcgtcgt ccaaaaatga ctaaaaacat caaattaaga aatgcgacaa
    29761 caaaagcgat tcgtcattat ttagatgata atgactttct cgatatcgaa acaccttacc
    29821 tagggaaatc tacaccagaa ggcgcaagag actacctagt tccttcacgt gtccatgctg
    29881 gacatttcta tgcgttgccg caatctcctc aattgtttaa acaattgctg atgaatgctg
    29941 gattcgaccg ttattatcaa atcgttcgtt gtttccgcga tgaagattta cgtggagacc
    30001 gtcagcctga atttacgcaa gtagatattg aaacaacgtt cttaacagca gaagaaatcc
    30061 aaacttacac agagggattg attgcgaagg taatgaaaga agttcgcggt atcgaagtca
    30121 cattgccatt tcctcgtatg acctatgatg aagcaatggc tcgctacggc agcgacaaac
    30181 cggatacacg ttttgatatg gaattgatcg atctaagcga tacagtcaaa gaagtagaat
    30241 tcaaagtctt ccaaatggct cttgaaaacg gcggtgtcgt gaaagcattg aatgctaaag
    30301 gcgctgcaga tcgctactca cgaaaagaca tggatcagtt aggtcaatat gtaggacaat
    30361 tcggagctaa ggggcttgca tggctgaaag tagaagaaga cggattgaaa ggtccaattg
    30421 ctaaattcat gggagaagca acagaagcaa tcattaaagc aacagatgca aaaccaggtg
    30481 acttgctgat gtttggcgca gataaaagtg aaatcgtcgc tgctgcatta ggtgctatcc
    30541 ggacacgttt aggaaaagaa ttaggactaa ttgatgaatc gaaattcaat ttcttatggg
    30601 taactgattg gccacaattt gaattttcag aagaagaagg tcgatatgtc tctgctcacc
    30661 atccattcac gatgccaaaa gaagaagata tcccgttatt ggctgaagac cctgcaaaag
    30721 tctatgcaga agcttatgat atcgtcttga acggttatga actaggcgga ggttctatcc
    30781 gtattcatac tcgtgagctt caagaaaaag tactagaaac tctaggcttt acaaaagaac
    30841 aaatggaaga acaatttggt ttcttgttaa cagctttaga ttacggattc cctccacatg
    30901 gcggtattgc attaggattg gaccgttttg caatgttatt agccggagaa gataatattc
    30961 gtgaagtcat tgcctttcct aaaaacggaa aagcagcaga tgtgatgagt gatgcaccaa
    31021 gtatggtttc gcctttacaa ttgttcgaat tgaatattga tgtaacgaat atagaagaat
    31081 agccgataaa aaggtaaaaa ggatttgtcg aatacgcttc ttttgacaac cttgatttaa
    31141 ttgaaaaaca ctttataatg aagccataac atataaagcg gggcttttcc atgaaaaaaa
    31201 cattcgaaaa ttgggcttat catgattaca cgaccaagct ttcggtatct atcgtttatg
    31261 cgttgatcgc atcagtcgca ttgaatttct tttatcagcc aggacatatt tattcaagtg
    31321 gtattactgg tctagctcag attttgacga ctttgtcaaa atcactgata ggattcgaag
    31381 taccggtatc aacaactttg tatttactga atataccttt gtttgtattg gcgtggttaa
    31441 aaatcgggaa gaagtttaca attttcactt ttatcacagt cactttgacc tccctttgta
    31501 tccatatttt accggaaaca gtcctatcta ccgatccaat catctgtgcg ttgtttgggg
    31561 gggctgtgat gggactaggt attgggctag cgttgagaaa cggcctatca tcgggcggtc
    31621 tagatatcgt gagtatcacg attcgtaaaa aaacaggaaa gtcgattggt tctatttcga
    31681 tctacttcaa tgcattgatc gtttttgttg ccggaattct atttggctgg caatatatgt
    31741 tttacagtac gttgtctatt ttcgtcagtg gaaaagtaac tgatgtcgtt tatactaaac
    31801 aaaagaagat gcaggtaatg atcgtaacga aacatccaga aaaagtagta aatgaaatcc
    31861 aaaactgtat gcgtagaggg atcacgatca tcaatgaagc agaaggtgct tataagcatg
    31921 ataagcagac agtattgatg acgatcgtta cacgatacga actgcctcta cttagagaag
    31981 caatgaaagc ttctgatcca caagcttttg taagtattgc agaaaacgtc cagatacttg
    32041 gtcgtttcta tgaggatggc atttagatcg agtttgacaa aaattcatga aatactgctg
    32101 tagacagacg ttcgtttgtc tgcagttttt ttctaccata gtagaatcag caaagaaaaa
    32161 ctgctttttt atttgaaatt ttcttaaagt tgaaaaaaag catgtttatt tttttaaatt
    32221 tatgggatga tagaaaaaaa tagtagagaa aggacacgat tctacttgct attttcactt
    32281 aatacccgta tattacgaat gaaaaaagtt tatgaaaagt tggaatattg aatggaaaaa
    32341 caaaaaaggt taaaaaacag ccgctacatc actgggtttg atgggctccg ttcattagct
    32401 gtggtgggtg ttatcttcta tcaccttctg ccaactagta tgaaaggcgg ctatttgggc
    32461 gttccgattt ttttcgtcgt atctggttat ttgatcacgg atctgctgcg gcaggaatgg
    32521 gaacaaaatg gaaaaatcaa tatttggcaa ttttatgtca gaagaatgaa gagactctat
    32581 ccggcacttg tttttatgct tgtgacagct tcggcttata tcactttgtt tcagcgaggg
    32641 ctgttaaaca atcttcgagg agttgtggtc agcagcttac tgtatgtcaa caactggtgg
    32701 caaatcaata acggtttatc ttatttcgat cgatttgcta atgagtcacc gtttacgcat
    32761 atctggtctt tagcagtaga aggtcaaaac tatttggttt ggccgatctt gtttgtattg
    32821 ttgatggtat ttgttcgtaa aagaaaatgg atcttttata ctgtgttagg tacatccttc
    32881 ctctccgcca tcttgatgat gatcttgtat acaccaggag gcgatccaac ccgtgtttat
    32941 tacggtaccg acactcgtct gttttctata tggttgggaa gtgcgcttgc gtttgtttgg
    33001 ccaagtacgc gtttaaagaa aaacatcccg aaacaagcga aaaatattct gaatatcgct
    33061 ggaggaatct cgcttttggc actgattctt tgcttcttct ttttagatga tcattacaat
    33121 ttcatctatt acggaggaat gctcctagtg agtctgttat gcgtagtgct agttgcagtg
    33181 acagcacacc ccggagcaag tttgaatcga tggctgacaa atccagtatt tacttggatt
    33241 ggcaaacgaa gctatggcat ttatctgtat cagtttccag ttatgatttt ttatgaagca
    33301 aaggtgactg atattgccga tcatgttttg ttgcatactt tagtggaaat cacattgatt
    33361 ctaatcatca gtgaactttc ttatcgatat gtggagcgtc cgctagcacg ttttgattat
    33421 ggtcgtacat tccaagtgat gaaaggatgg ttcagcaaac cgatcatcag taagagcaaa
    33481 ccatgggttg ttccaggcac attacttgta gcaattgcac tcgttggttt cgttacggca
    33541 ccgaaaaacg ctgtaacagc cgatcaaaaa caactgcagg caaaaatcgc tgaaagtaaa
    33601 aaattagcag aagaaaccag aaacaattca gaaaacacca ctagttcggt tgaccaagcg
    33661 atccttgata agtacgagtt gtccaaggca caagggaata aggcacaaaa attagaacta
    33721 acggcatttg gtgattcagt tatgttggat gctgcaacgg atctacaaga actttatcca
    33781 aaagtagtgg tagatggaga tgtcggacga caattatatg ccagtgtacc gtacattgaa
    33841 aaattaaagg aacaagattt gttgaaagac actgttctga tcggactagg aacgaatggc
    33901 tcattctcgg aagctcagtt tgatagtgtg atggatgcaa tcggtgatcg aaaagtatac
    33961 tgggtcaatg tccgagtacc gacgaagcgc tggcaaaatg acgtgaatgc gatgcttgaa
    34021 cagatgacga aaaaatacga caatatgacg ctgattgatt ggtacagcta cagtaatgac
    34081 catagtgact ggttttatga tgatcaagtc catccaaatc ctgaagggat gacacattac
    34141 attcacttag tcagcgaagc cttgctagga ggaagttcaa gtacgaacag cagtgagaca
    34201 aacgtgacag aatgatagct acaaaaaact tcaggcaaaa acatttgttt ttgcctgaag
    34261 tttttttata tttaccgtgt ttttaacatt catatagatt caggaagcaa aacggttgag
    34321 tgtgtctgta aataacgttc tttttgtttt cgggtgagtt ttttgaatgc agcttctgct
    34381 tttgttgctt cgcttcttga agaaaatact tctgcatgga tcatcttaac aggtcttctt
    34441 ttttgaagac gagtatattt agcaccagta ccattgttat gttcctctaa tcgtctagaa
    34501 agatcggttg tatatccacc ataaaatgtc tgatctttac agaataatac gtaaaaataa
    34561 tgttcatttg ccatagagca tctcacgaat ctctgcagtg tattctccat ccatattgta
    34621 agtgatcaat ggtggagcga ctttgaatcc gtctaatttg ccttctttga ttccttcaac
    34681 taacaaaata tttgcctctt ttccaggttt tgggtaaaca aattggatcc ttttaggtgc
    34741 aattcgatag tcttccatga cctgaatgat ttctaagaac cgatcaggac gatggaccat
    34801 agtaaagcgt ccatttgttt ttagtagctt acttgaaata tcgatcactt cttctaacgt
    34861 cgtatgaatc tcatgacggg cgattgccag gtgtttatta gggttcgttt tatttgttgg
    34921 cagccctttg aaataaggtg gattacagac gactaagtca caagaattat gtcgaacgac
    34981 agataagctg tctttcagat cgatggtgtg gacagtcatt tgttcattta aatggttaag
    35041 cgcgatacta cgtcttgcca tgtctgctag acgaggttga agttcgatag cgtcgatagc
    35101 cgcctgggtt cgtttactta aaaaaagtcc aactgcgcca tttcctgcac aaaggtcaac
    35161 aatcttccct tttttaggta cacgggaaaa gtgagcaagc agcacagcat ctaaagaaaa
    35221 agaaaacact ttggaacttt ggataatctt gatatcttct gcgtataatt ggtcgattcg
    35281 ttcgtcttcg tgaagcatgg attccctcca aaatgaaaaa aatagtcgtc tgtattatac
    35341 tatttttatt aaaaagaacc aaggtggctt gtcaaacctc tattttttat gcatactatg
    35401 accataagaa gggaaaggaa gagactatgt tttatcgatt tatgcgcggg gtagttcgag
    35461 tactattagc cttagttaat ggaaatgccc gctatgagaa taaagaagtt ttaccgcaag
    35521 atgagaatta catactagtt gctccccatc gtacttggtg ggacccatta tatctggcgg
    35581 tagctgcagc acccaaggaa ttcagcttta tggcaaaaga agaactgttc aaaaatccag
    35641 ttctgcgatt tatattaact cgatccaatg cttttccagt taaaagagaa aatccaggac
    35701 caagcgtcat taaaacacct gtaaagattt tgaaatcgac aaatttgagt ttgatcatgt
    35761 ttcccagtgg gacaagacat gctacagaac ttaaaggcgg aatggcattg atttccagaa
    35821 tgtctaaagc gaaaatcgtg ccagctgtat atcaaggtcc gttgacactt ggggatctgt
    35881 tcaagcgtca gagagtgaca gtgcgtttcg gcgaaccaat cgacttatca gatatcaaga
    35941 aaacggataa agaaggtatg gcggagatcg aacgacgaac acaagcagct tttgacaaat
    36001 tggacaaaga agtgaaccct gatttcaaat atgaagtcaa aaaaaaataa aatgataagt
    36061 ttatgcgtgg gcggtctttt gaatctactg tcctgttcag ttcagacttt ttcaatgaaa
    36121 acgagggaga gacgtcaagt ctgccctctt tttgcgttcc aatatgtaaa atagaagaca
    36181 ggtgtcatgt aatggatcaa agtgttataa taattagaag gacatttttt gaggtgattg
    36241 aatgcgtttt ttacatacag ccgattggca tatcggaaaa aaactacaag gttatgactt
    36301 gattgaagac cagcgtcatg tactatcttc tatactagat atcgcgaaag aagaaaaagt
    36361 agaggcaatc gtgattgccg gagacttata tgataggagt gtcccagctg ttgatgccgt
    36421 agaattatac aatgaattga tggtcgattg gaatttaaag gaaaagatcc cgatttttgc
    36481 tatatcagga aatcacgata gcagtacgag actttcagcg ggtacgccat ggttcagtta
    36541 ttcgaactat tttttgcgaa cacagctagc agaagcattt gaaccagtcg agtttaaaaa
    36601 cgtacaattt ttctttcttc cttactttga accaattgca gcgagattat attttgaaga
    36661 tgacaatatc cgaacgatca agcaagcggt agccaaggtg atagataaaa tgcaggagct
    36721 atttgattca gaaaaaaaac aagtattgat tagccatttt tttgtaatgg gaagtgtaaa
    36781 atctgattcg gaaacgaaaa tcgaagtagg cgggttggat tcgatccctg gtgaactact
    36841 tacaccgttt gattatgtag cactaggaca tttgcataat caagctgctt tgagacagga
    36901 taatgctcgc tatagcggtt ccccattgaa attctcttta tctgagatca accaaaaaaa
    36961 aggcgtatgg atcgttgacc tgacaacagg cctggagttg aattttaggg agatcgaacc
    37021 cctgcatgat attgaagaaa taacagcgag tttcaaagaa ttgctggatc ctgcctttta
    37081 tcagtccgtc aatcgggaga attacatcta tatctatctc actgatagaa cgatcatccc
    37141 gaatatgatg aaccagcttc ggcaaatata tcctcgtatt ttggggattg aacgggcaaa
    37201 cggtcgagca gaacaaacaa agcgcaaacg aaaagtacta gaaatgaaag atcctcaaaa
    37261 attagcagaa gcatttttta gtgaaatgac ggaagagcag ctaactgcaa accaagaaaa
    37321 atggctcgga gaaacgttgc aagaaatcat ggaaatggaa tgatcaagaa tgaaaccgaa
    37381 aaaattacaa cttaaaaatt ttggtccttt tatcaatgag accgtagatt tttcacgttt
    37441 gacagaagca ccgctttttt tgatcagcgg gaagacagga gctggcaaaa cgacgatttt
    37501 tgatgggatc accttcgcac tttttggtga aacttccgga agacttcgct ctggaaaaga
    37561 gatgcgttct ttgtttgcta caccagacga agaaacaagc gtgacttttt cttttgaaca
    37621 tcagcagatg agctatgaaa tcgaacgaaa accagaacaa gttttagcaa aacgaaaagg
    37681 aaacggaaca aaaaaacagg cagccaaagt aacgttgacc atttttgatg ggaaaggaaa
    37741 agaaataaaa caaatcacaa aacgtacaga agcagatcag ttgatcagag aactgatgaa
    37801 tctcgatgcg aaacagtttt ctcaaatcgt tttactaccc caaggagaat tccgtaattt
    37861 tttggtttca tcaagcagtg ataaagagac tgttttacgt catttattcg gtactcaatt
    37921 tttccaacag ttcaatgagc gattaaaaga aaaagcaaaa caacagcaac aaaaattgga
    37981 tcatttagag caagagctgc atcttctgca aaaaaggttt gttttagtca aagaagagga
    38041 accagcagca gggtttgaag ccgttttagc ccaatggaca aaccatcagg caatactcga
    38101 aaaacaaatc acggaagaaa aagaagctct aacggaactt caggaaaaac aaaagcaact
    38161 agaaaattcc tactatcgtt ttgaaagctt gaaaaaaagt tatgaagaaa aaacgatatt
    38221 aataaacaag caaaaggaat tagctgctca aaaagacgaa atagaggaaa agaaacgatg
    38281 gattgggtat tatgaattta cgcaacgatt agtcgaacca atccagcgcc tacaagaaat
    38341 aaaagttgaa aaaatagaac tgtcacaaaa aagtagtgag caaacaaaga aactagcgat
    38401 gactacgcag aattatgaag agtggcaaaa aaatgaagca aaccgcgtac gccgacaaac
    38461 aaaaatcaaa gaagctgctc aaaaactgca aaacaaacag atgatggtgc caattgtcga
    38521 atccttacgt agtaaaaaac aagacgcaga acaattaaca atttcattgg ataaagaaga
    38581 attgaatgcc gagaagctgt atcaaaatct cgctatctat caggaaagaa ctcgaattct
    38641 tcaagaaaat ttactggatc aggaaaaact gcaagaagat cgtcttgctt ttggacagtt
    38701 aaaatatcta gatgaaaagc agcaagaaat agaaaaacaa aaagcaaaag acactgggtc
    38761 gctcgcacag ataagagaag aactcaaaaa agcaactgaa gaaataacaa aaaatagtca
    38821 gctattgaaa gaacagacag aacaagcaga acagctgaaa agccaatggg caaagcagca
    38881 aattgctcgt cttcagcttt ttttgcgtcc aggggagcca tgtcttgttt gtggatcaac
    38941 ggaacacccc atacaacaat taaaacacga acagccaaat aaggaagata tcttaaaagt
    39001 agaacaacaa ctggcagaag ctgagtctgc tgtagaaaga actcagcaaa tcattgccaa
    39061 aagtgaagca catgaaaata atcttcaaca acaattatca gatttagaac aaaaaatcat
    39121 ggcaacgaat atacaattgg ctgaacaaga agaacacttg caacaggctt tgaaaaaggc
    39181 atttcctgag ataacagaac aaaaaacaaa aacaactctt gtagtaatcg aacaatcttt
    39241 agatagacga aaaagcaaga tggccaatgc aagaatagag ctggaagaaa taagacaaca
    39301 agcagaaaag ctcgaacaac aattagctgt ctacaaacag cagcagacag aaaaaaataa
    39361 tcagctccag caactgcaag gagaaatcaa aagtttagaa caacaattgc aagatacgga
    39421 tagtaactgt ttagaagagg agatacgacg acttgaaaaa cagttagaag aagactcgaa
    39481 tcaactagct gttgagcaga aagcaggagt gcaattgttg caagatcttc atacgcttcg
    39541 gacacagctt gatcttcaac atgaacaaat agaaaaaata gatgaaaaaa tagaaaagga
    39601 acaagaaaga atagctaaaa agttagaaag ccagtcgtat ttttcaacaa ttgaagaaat
    39661 ctgtgcattt gcacaacagc aagcacaata taaagaaaac caacgtacag ttacttctta
    39721 tcatgaagag caattagtga cagctgaccg gctgcgtcaa ttagaaaata tccagctaac
    39781 tgagacattc ccagatacag aagagatcag ccaacagtta gaagaaataa gacgtatgat
    39841 cgaaataaag caagaaaaga tatacggttt acaagaacaa aaaattagta atcaaaaaat
    39901 ctacgatgaa tgtctttcga tttatcacac aagccagacg aaattagatg agcttacgca
    39961 attacagcag ctttcgcaaa cattcaacgg agaaaatcct aagaagacta gtttggaacg
    40021 gtatgtgctt caggtttatc ttcaagaagt gttacaagta gcaaatgatc atctccaacg
    40081 tttaacgaaa aatcgttatc aatttgagct agcggatacc attggcagtt atcgagggaa
    40141 aaccggatta gaaatcaata tctatgatga tcaggcaggt atgtctcgaa gtgcgcatac
    40201 cctgtctggc ggtgagagtt ttattgcagc actttcttta gcattagctc ttgccgatgt
    40261 gatccagaca caagctggtg gtgtagcaat cgatgcattg tttattgatg aaggatttgg
    40321 gtcattagat gaagaagctt tggaaatggc aatggaagct ttagaaacga tcgaaagtga
    40381 aggaagaatg atcggcatca ttagtcatgt aagagaattg aaagaacgag tattacaaca
    40441 aataagaatt gatacagaag gtagcggaca aagcaaaata cgctatcaac ttggataaaa
    40501 agggagattg gtaactatga aatggagtat tccagaaaaa gtcattgcac gtggacgtca
    40561 atacttgcaa gaaggccgag tgttatcggt tgtccctgac caagaagaaa acgtgtggca
    40621 tgcagaagtc ttgggcagtg aattgtatat ggttgatttg gatggtacag caaaagagat
    40681 agattactgc caatgtccct attgggagga acataaatat tgtaagcata cagttgctgt
    40741 ggaactttat ttacggtcga aaaatctatc tcgtatcatg aaaaaagacc agccaatgct
    40801 agaaccggta aaagcaacaa gtgagggaga aatcattgca aaaggttttt ctcgtctaaa
    40861 aacacaaaac aatcctcaag cggtgcaacc tttgattatt gaataccaag tagaaacgat
    40921 tgagactaat cagtatcacc cagagctttc tattttggca ctttacttgc gtatcggttc
    40981 attggcatca ccgaaaaaaa catatatcgt aaaaaacatc tatgattttt tacaagctta
    41041 cacagaaaaa gaaacttaca ctgttaataa acagtaccag tttcgtctag ataaacgggc
    41101 gtttcaaaaa gaagatcaaa aaatactcac gcatcttgca ggaagtgctc agacgcatca
    41161 attattaggt acgaacggct tacaagtcaa aggaaaacta gataaaagat atctgcttct
    41221 cccagtagaa caagcccgct tcctgttaga gcagatgaat cagactacac gtttgcatct
    41281 gcagataaat gaacaaaacg atcaaggaat acaattttcc gatcaaaaca aacctttatc
    41341 attcaaagtg gaaaaatttg gagaagatta tttgctgcaa gcattgaatg attttgattt
    41401 tttctttatg cattatcagt ggggagttat ccaagggaca ttttattctc tgacgaaata
    41461 tcaacaaacg gtttatcaaa cttggaaaca attgattcgc cgattggaga aaccagaagt
    41521 agtgttcaag aaaaaagaat taccgtcttt gtttaaagag attatccctc tgctgtcaga
    41581 gatcggcgac gtagctattg cccctgcagt agaagaggaa gtagcagata tccccgtcga
    41641 atttattttc tttttccgaa aagcaaaagg aaaaatccaa gctcgagtcg atttcgtcta
    41701 tggcgaagtc atctattcga ctgacccgaa gcatgaggtc tcgacagaac aggcttttca
    41761 ggtgttacgt gacacagtag aagagaaacg agtacttgat ctattcagaa tgtatcgcta
    41821 ccagaaaaat gaaactggtt atgaacgtcc attgccaagc ggggaagaac tatatgcctt
    41881 tttccgcaca gaactatctg tcttccgtca gtttggtgaa gtcagacttg gaagaaaatt
    41941 gcgagagttg tatttagatg cgcataaaca tcaaccgaaa atcgaagtaa ccgagaccgg
    42001 ttcatggctc gatattcgat tcgatgtgac agggatagag gagcaagaaa tcgatcatgt
    42061 cctgcaaagc ttgctgagaa atgatgcttt ttatacatta gaaaatggtc aggttctttc
    42121 ttttgattca gaagaatttc aacaaacaag ccaagtgttg caacaattac gggaatcgat
    42181 acgtacagaa gaaggaacga tccatgtacc taaaaatcaa ggattgatca ttcaaaatca
    42241 attggaaaaa agcaatgcta cttttagtga atcgttccaa acgatggtac aagatctcat
    42301 tcatccagaa agataccagg cacagcttcc caaaggattg aatgcaacga tgagagatta
    42361 tcaaaagcaa ggttttcgat ggttgaagat gttaggtcat tatcagtttg gcggtatttt
    42421 agccgatgaa atgggacttg ggaaaacact acagacaatt gcctttttac tttcggaaaa
    42481 agaagaacgg aaaagcttct ctgctttgat cgtagcgcct gcgagcttaa tttacaactg
    42541 gcaggcagag gtgagaaaat tcgccccttc tttaagtatc caagtaatca atgggaataa
    42601 aaaagaacgt gaagaattac tggctaaaga cacagatatc cgagtgactt cttatgccag
    42661 cttaagacag gatctagcta attatcagtc gcaaaaaatt gattatttga ttcttgatga
    42721 ggcacaaatg gtcaaaaata gcagtacgaa aactgcacaa gcattgagag aattagctgt
    42781 acctcagcgg tttgcactga gtgggacacc aatcgagaat aatttagaag aattgtggtc
    42841 attgtttgca acgattatgc caggattttt cccgactaaa acaaaattca gggaacttgc
    42901 cgcagaagaa attgcgcaaa tgattcgacc gtttatttta cggcgagata aacaaactgt
    42961 gttgaaagac ttgccggaaa aaacagagat gaatctatat tctgcactta cagaagaaca
    43021 aaaaactgtt tatttagctt acttgcgtca aatgcgtgaa gaaatcacat caatggattc
    43081 tgaagcattc aagaaaaacc ggatcgggat acttgctggt ctgacaagac tgcgacaaat
    43141 ttgctgcgat cctcgtctgt ttattgaaga ttaccaaggc ggttccggca agctcgaaca
    43201 agtaaaagat ctactggtag ctgccaaaga gaacaaacga agagttttgc tgttttctca
    43261 atttacaggg atgctgacta tcttgcaaga agagttagcc gagctaggga tctcgacatt
    43321 ttatttaaga ggcagtacga aaccacaaga tcggttaagt atggtagacg catttaatgc
    43381 tggcgaaaaa gatgtttttc tgatttcctt aaaggctggg ggaactggat tgaatctcac
    43441 gggagcagat acggtcatct tgtatgacct ttggtggaat cctgccattg aagaacaagc
    43501 tgctggacgt gcacatcgga tcggacaaaa aaatgtggta gaagtgtggc gtatgatctc
    43561 tgaaggaacg attgaggaac gcatggattc actccagcaa gaaaaacgcg aactgttcca
    43621 aaaagtgatc caaggtaatg aagaacagtt gacgaaactg actgaagaag acatccgcat
    43681 gattttaagt gtaggagaaa tcgaggagta agagactcga aaaagcgttc agtaatgggt
    43741 aagaaagcct ttctaaagaa gcgattgttt atttgattta aaaaatagca aggaggctgg
    43801 gattccattt tatcccagct tccttgctat cattttttta tttctatttt taaaggttat
    43861 acaaagattg tcttcattgt ctctaacagc tgatcaaaag aagaaatctc gatatcgcaa
    43921 attggagtct gttcaatttt gcgttctcga tggttgaacc aaagcgcttg ccagttagca
    43981 ctttttgcac ctagaacatc attgtcgtaa ttatcgccaa cgtataaggt ctcttctgga
    44041 agcatatgaa attctttctc agctagctga aaaatctctt tttctggttt ttggaaacct
    44101 gttgcttgag aaatgagcat attgcgggaa ggaatccaat tgttcaattg aagttgcttc
    44161 agtttttttg tctgatggtc agtcggccca tttgtgataa ttcctaatgg gacattcttt
    44221 tccttcaaga aatcgagggt ttttttgacc tcctcatgca agcagatatt atcaagttct
    44281 tcctcgtaaa ttttttgaaa taagaggcca tcttcttccg taatatgagg gtagtctaaa
    44341 tcttttagtg attggctgat ccgatgtgct cgcatatatt ctagtgtcca atctcctgcc
    44401 attacttttg ggaagttttc atcactatga tggcggaacc ggatatatag tggatgcata
    44461 tctgcatctg aaacaagcgg aacgactctt ttgaccgcgt ttcgaaatgg ctgctgctga
    44521 tcatacatcg tatcatcaac atcaaacaca actgatttaa tcaaaatttt acacgccttt
    44581 cggttttcat tgattttttc acaaatcctt gactattgta acagaaaaaa ttgtctaaaa
    44641 aagctgataa ataaaggttt ttcttactat atttctattt ccttgacaga ataaggtttt
    44701 tatatttcag tttttttcac taaaaatcgt gaaacacgtc aataagtttg atgaacaaat
    44761 taatcattat ggtagaagtc gattgattta ttttaatgca atctctgtgg aattttcgaa
    44821 agatttattt ttttaataca gtctcaatat aactattcaa acataaaata acgttatttg
    44881 atttatttgt ttatagtaaa ttttatttag taatcattgc tttaaaatac tacgcttata
    44941 taacagagaa aataagcgtt ataataaaga acagtattat ttaaaatgac agaaaacatt
    45001 actattaaag aaataaaaaa cataaaaaat cttaagaaat cggtttcatt tagcttttga
    45061 gataaaataa tagtacagtt ttatacgctg tttcatatgt ttagaaaatt tgaattagat
    45121 ttgaaaactc taatttttaa atgataaaaa aagcgagaat gctggtttta ctgtatatta
    45181 accttagata actttcattt ttttaaaaaa attatgaaaa aaaggtagct atttaaaagt
    45241 tgattatgta taatagaaac agatgggaga ttattatgat cttctgttac aaaaaagtga
    45301 ttcttatcac gaaaaggagt ggtgcacgtt gttaacatta tatacttccc caagttgtac
    45361 ttcctgcaga aaggctcgtg cgtggttgca agaacatcag attccatttg ttgaacgtaa
    45421 tattttttca gaaccattga acagttctga attaaaagcg attttacaaa tgacagaaga
    45481 cggaacagaa gaaatcatct cgacacgttc caaagtattc caaaaactga acatggattt
    45541 agatgatctg ccgttacaag aattattgga acttgtgcaa aacaatcctg gattgcttcg
    45601 ccgtccaatc atgattgatg ataaacgtct gcaagttggt tttaatgaag atgaaattcg
    45661 tcggttccta ccgcgcgatg tacgacaatt agaattacgt caagcacaat tgatggctgg
    45721 attatagaag gataatacga aacttggaaa agactattac aaagatagtt tttccaagtt
    45781 tttttgtttt tacaaaaagt attcttgatt agatgtataa aaaggtagta gaagatgatg
    45841 attgcacgaa aaatagagag ggaaaattag ctgaaaagtc ggctatatag tattcgtctg
    45901 ccaaaaatgt tatagttaaa taagatagct tgaaaggttg aaaaatccgc tggttttatt
    45961 aaattcgctt aacagcgaga ttttttttct ttacatttaa gctgatttcg ctacaatgaa
    46021 tctatattaa acattgtggc aaagtgaggt gtagcaaaat ggaaatggaa catattaatg
    46081 aaaatacgat ccgtgtacta attggtcatg aagatttaga ggaacggggc gtttcatttt
    46141 tggatttact tggcaatcac aaagaaatcg aaaatttctt ttatagtatt ttggaagaag
    46201 ttgatatcga agaggagttc cgaggaagcg aagctgtcac tttccaagtt ttgccaaaag
    46261 gagacggatt agaactgttt atcagtaaaa atcttccgga tgaagaaatt tctcaatcag
    46321 aagattctac tgttgattca gaagatgtga gtgactttct tcgtcagcag attattggca
    46381 gcgatccgga ggaagaatta tctggaagcg ccctagaaga acaggaaaga caagcaactt
    46441 ttgcttttga agattttgaa caagtgatcc aacttgcaac agatgtcgaa ctagagtctg
    46501 cttggactga tttgtattta ttcgaagagc gctattacct agtcgttcac ttctggatgg
    46561 aaaatctcaa ccaagcggat gtagagaatc agattgctcg aattttggag tttgctgaaa
    46621 agagcgatcg tacaccagaa acattgaatg aatatggaca atgtctgatg gcgcgtaatg
    46681 ctatcgagcg aacgaaattt tattttgtat aaatcagcat tatagtaaga gactgtgaca
    46741 aaaattgtgt cgcagtcttt ttttcgtata aatgtgttca aaagcttgca tctgcggtaa
    46801 caagcccaac caaacgaaaa atattgggaa ctatttttgt ttagctgttc ttattacttg
    46861 atgcagaata gctttttcac gccctctttt ttcgtgtaaa taaattctta gacgtaaata
    46921 agttcagagg gagaaaaatg ctgcttgcaa tgaatgaata taataagtgc tgttttgcta
    46981 aacatgcaga aaaaggaaag aaatattatt gtcgtggctg tcgaaaaaga gtgattttga
    47041 aaaagggaaa gaaaaaatgt gctcattttg cccatcaaaa atcagataac tgttcggtct
    47101 ttagtgaaag agaatcaaaa gagcacttac agttaaagga atgcttcatg gattggctgg
    47161 gacagtcagt agaaccagta tttttggaag cctatttgcc gaggttgcgg caaaggccgg
    47221 atattttgtt ggcgaatcta gcaatagaaa tccaatgttc tagactgtct caccagagat
    47281 ttattgaaag aacccaaaat tatttgaata acagttacca agtatggtgg atacttggta
    47341 attcttttct tggacaatcg caattctcat tgatagaaaa aagttgctgc tactataata
    47401 gaaaaagagg agtgcattgc tggaaagctg atctcaagaa acaaaagctt tatttgtatc
    47461 atcatattac agagactgta tcagggcata tttccttttg cagtagctgc tggacttttt
    47521 ctagtaggga ccttaaagaa atattcacta gtaacgaaat taaaatgaac caaatgaaaa
    47581 aaatagaaaa agtgagtgaa gatgggaaga aatggctgac acgccaactt attcacaagc
    47641 aaaagaatac agtatccata caagaacaat gctacttgcg tcataaacat cttttgcatc
    47701 tatcatcgtg gatctatcaa aaaagtcggt ttttctttta tttacaagaa caggtttttc
    47761 tttatcgaat gttgtacgaa gaagccttgg aaaaccagaa agttcctgat ttcaactcat
    47821 ggctttgcca aataaaagaa cataaaaggg aatggctttt tcctatgatt gatgaagaga
    47881 tggtctatca gcagtttttt aacgaatgca tacacttgtc ttctttgaaa aaatagtaag
    47941 tataaaaata ggctaggaat cggttttcta tgtaaaacaa gatttagtta tgctaaaatt
    48001 gatggaagca gacacatgaa tgagtagaaa agttagatgc tgtgacatgt ggatgttaga
    48061 ctatgatcta ttttgataga tagagctaag ggaagaaaag gagtggattt catggaagta
    48121 aagcagttgc caaaacgaga agaattgcct gaaaatttaa cttgggactt gaccaagatc
    48181 ttttcaagcg accaagagtt tgatgagaaa tatttggaat tatcagaaga gttaaaacaa
    48241 tctgaaaaac acaaaggaac acttgatcaa ggcgcttctc aatttttaaa tgcgattgaa
    48301 ttcgtattga gggtttatcg ccaaactgaa gtcatttatg tatatgcgca ccttaaaaac
    48361 gatcaagaca ctggaaatac agattaccaa gcgctttatg caagagcaag cagtctgttt
    48421 tcgaaagtta gtgaagccgt ttcctggttt gaaccagaaa tattgcaatt gtcagatgac
    48481 cagatttggc aatatttcaa agaagaacca aaattggaag tctatcgcca ttatatccag
    48541 caaatagtag ataatcgagc ccatgtctta tctgctgagc aggaatctct tcttgctgga
    48601 gcaggtgaaa tctttgatgc ttcaagtgat acatttgctg ttttgaataa tgcagatcta
    48661 gtttttccaa cgattgaagg agaaaatggt gaaatagtcc aattatctca tggcgtgtat
    48721 ggtcagttgc tagaaagcac ggatcgaagg gtgcgcgaag cagcatttaa ggggttgtac
    48781 agtgtttacg aacaatttag aaatacattt gcttctactt taggcacaca tataaaagga
    48841 cataatttta aagcgaaagt ccgtaattac agctctgcca gagaagcgtc tttgagcaat
    48901 aatcatattc ctgaaagtgt atacgatact ttggtagacg tggtaaacaa gcatttgcct
    48961 ttgttacatc gatacatgga attacggaaa cgtttattag aagtggaaaa actgcacatg
    49021 tatgatcttt atacaccggt cttaggggaa gctccaatta cctttacgta cgaagaagca
    49081 aaagaaaaag ctttagaagc actgaaacca atgggtgaag aatacatggc catcgtagaa
    49141 aaagcattct ctgaacgttg gatcgatgtt gtcgaaaata aagggaaacg aagcggtgct
    49201 tattcttcgg gaagctatga cacaaatcca tatattttat tgaattggca tgatacgctg
    49261 gatcagctat ttacgcttgt ccacgaaatg ggacatagtg ttcatagtta tttcactcgt
    49321 tcgaaccagc cttatgtgta cggcgactac tccatctttt tagcagaaat tgcttcgaca
    49381 acgaatgaaa atatcctaac ggagtattta ttggaaacag aaaaagaccc tcgtgtacgg
    49441 gcttatgtac tcaaccatta cttggatggg tttaaaggaa cagttttccg ccagacacaa
    49501 ttcgctgaat tcgagcattt catgcatacg gaagatgaaa aaggtgtgcc attgactagt
    49561 gaatacctaa gtgatagtta tggtaaattg aatgcaaaat attatggtcc agcagtcgaa
    49621 gaagacccag aaattaaatt cgaatggtca cggataccgc atttttatta taattactat
    49681 gttttccaat actcgactgg cttttctgct gcttcagcac tggcgaagaa aatactaaac
    49741 caggaaccag aggcactaga gaactacttg gcttacctaa aagcaggtaa cagcgattat
    49801 cctgtggaag taatgaaaaa agcaggagtt gatatgacac aggctgcata tattgaagat
    49861 gcaatgtcga tgtttgaaca acgtctaaat gaattagaag aattgatcga tcgtttataa
    49921 ggctttctat ttttgatttt ctcgaataga aacgaaaaaa atacgtcggc tctcttgtaa
    49981 gggagtcgac gtattttttt a
//
LOCUS       DACCYH010000009        47456 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.009, whole genome shotgun sequence.
ACCESSION   DACCYH010000009 DACCYH010000000
VERSION     DACCYH010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.009"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..2387)
                     /gene="esp"
                     /locus_tag="IVY85_000502"
     CDS             complement(<1..2387)
                     /gene="esp"
                     /locus_tag="IVY85_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP8043540.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVS"
     gene            complement(3004..3438)
                     /locus_tag="IVY85_000503"
     CDS             complement(3004..3438)
                     /locus_tag="IVY85_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043541.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3455..4660)
                     /locus_tag="IVY85_000504"
     CDS             complement(3455..4660)
                     /locus_tag="IVY85_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP8043542.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5354..5740
                     /locus_tag="IVY85_000505"
     CDS             5354..5740
                     /locus_tag="IVY85_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8043543.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5834..6025
                     /locus_tag="IVY85_000506"
     CDS             5834..6025
                     /locus_tag="IVY85_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8043544.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6539..6871
                     /locus_tag="IVY85_000507"
     CDS             6539..6871
                     /locus_tag="IVY85_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8043545.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            7167..7322
                     /locus_tag="IVY85_000508"
     CDS             7167..7322
                     /locus_tag="IVY85_000508"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP8043546.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            7598..8938
                     /locus_tag="IVY85_000509"
                     /pseudo
     CDS             7598..8938
                     /locus_tag="IVY85_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9179..10795)
                     /locus_tag="IVY85_000510"
     CDS             complement(9179..10795)
                     /locus_tag="IVY85_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8043547.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKKIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            10913..11131
                     /locus_tag="IVY85_000511"
     CDS             10913..11131
                     /locus_tag="IVY85_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8043548.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            11326..11787
                     /locus_tag="IVY85_000512"
     CDS             11326..11787
                     /locus_tag="IVY85_000512"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8043549.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(11980..12219)
                     /locus_tag="IVY85_000513"
     CDS             complement(11980..12219)
                     /locus_tag="IVY85_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043550.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(12243..13766)
                     /locus_tag="IVY85_000514"
     CDS             complement(12243..13766)
                     /locus_tag="IVY85_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8043551.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(13784..13906)
                     /locus_tag="IVY85_000515"
     CDS             complement(13784..13906)
                     /locus_tag="IVY85_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8043552.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(13936..14919)
                     /locus_tag="IVY85_000516"
     CDS             complement(13936..14919)
                     /locus_tag="IVY85_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8043553.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(14943..15092)
                     /gene="rpmG"
                     /locus_tag="IVY85_000517"
     CDS             complement(14943..15092)
                     /gene="rpmG"
                     /locus_tag="IVY85_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8043554.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(15113..15490)
                     /locus_tag="IVY85_000518"
     CDS             complement(15113..15490)
                     /locus_tag="IVY85_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043555.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(15522..15701)
                     /gene="rpmF"
                     /locus_tag="IVY85_000519"
     CDS             complement(15522..15701)
                     /gene="rpmF"
                     /locus_tag="IVY85_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8043556.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(15716..15985)
                     /gene="rpsN"
                     /locus_tag="IVY85_000520"
     CDS             complement(15716..15985)
                     /gene="rpsN"
                     /locus_tag="IVY85_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8043557.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(16508..18280)
                     /locus_tag="IVY85_000521"
     CDS             complement(16508..18280)
                     /locus_tag="IVY85_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8043558.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(18234..19988)
                     /locus_tag="IVY85_000522"
     CDS             complement(18234..19988)
                     /locus_tag="IVY85_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8043559.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(20098..20667)
                     /locus_tag="IVY85_000523"
     CDS             complement(20098..20667)
                     /locus_tag="IVY85_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP8043560.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(20685..22109)
                     /locus_tag="IVY85_000524"
     CDS             complement(20685..22109)
                     /locus_tag="IVY85_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8043561.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(22111..22779)
                     /locus_tag="IVY85_000525"
     CDS             complement(22111..22779)
                     /locus_tag="IVY85_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8043562.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(22909..23493)
                     /locus_tag="IVY85_000526"
     CDS             complement(22909..23493)
                     /locus_tag="IVY85_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8043563.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(23824..24057)
                     /locus_tag="IVY85_000527"
     CDS             complement(23824..24057)
                     /locus_tag="IVY85_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP8043564.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(24072..25022)
                     /locus_tag="IVY85_000528"
     CDS             complement(24072..25022)
                     /locus_tag="IVY85_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8043565.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(25019..25861)
                     /locus_tag="IVY85_000529"
     CDS             complement(25019..25861)
                     /locus_tag="IVY85_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8043566.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(25863..26582)
                     /locus_tag="IVY85_000530"
     CDS             complement(25863..26582)
                     /locus_tag="IVY85_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8043567.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            27274..27432
                     /locus_tag="IVY85_000531"
     CDS             27274..27432
                     /locus_tag="IVY85_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8043568.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            27504..28730
                     /locus_tag="IVY85_000532"
     CDS             27504..28730
                     /locus_tag="IVY85_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8043569.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(28818..29210)
                     /gene="rpsI"
                     /locus_tag="IVY85_000533"
     CDS             complement(28818..29210)
                     /gene="rpsI"
                     /locus_tag="IVY85_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8043570.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(29226..29669)
                     /gene="rplM"
                     /locus_tag="IVY85_000534"
     CDS             complement(29226..29669)
                     /gene="rplM"
                     /locus_tag="IVY85_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP8043571.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(30188..30655)
                     /locus_tag="IVY85_000535"
     CDS             complement(30188..30655)
                     /locus_tag="IVY85_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP8043572.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(30775..32313)
                     /locus_tag="IVY85_000536"
     CDS             complement(30775..32313)
                     /locus_tag="IVY85_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP8043573.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            32393..33079
                     /locus_tag="IVY85_000537"
     CDS             32393..33079
                     /locus_tag="IVY85_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP8043574.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(33280..33807)
                     /gene="msrA"
                     /locus_tag="IVY85_000538"
     CDS             complement(33280..33807)
                     /gene="msrA"
                     /locus_tag="IVY85_000538"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8043575.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(33891..34568)
                     /gene="pgmB"
                     /locus_tag="IVY85_000539"
     CDS             complement(33891..34568)
                     /gene="pgmB"
                     /locus_tag="IVY85_000539"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8043576.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(34565..35695)
                     /locus_tag="IVY85_000540"
     CDS             complement(34565..35695)
                     /locus_tag="IVY85_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8043577.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(35695..37878)
                     /locus_tag="IVY85_000541"
     CDS             complement(35695..37878)
                     /locus_tag="IVY85_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8043578.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(37875..38900)
                     /locus_tag="IVY85_000542"
     CDS             complement(37875..38900)
                     /locus_tag="IVY85_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8043579.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(38945..39772)
                     /locus_tag="IVY85_000543"
     CDS             complement(38945..39772)
                     /locus_tag="IVY85_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8043580.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(39769..40836)
                     /locus_tag="IVY85_000544"
     CDS             complement(39769..40836)
                     /locus_tag="IVY85_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP8043581.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(40862..41698)
                     /locus_tag="IVY85_000545"
     CDS             complement(40862..41698)
                     /locus_tag="IVY85_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8043582.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(41695..42564)
                     /locus_tag="IVY85_000546"
     CDS             complement(41695..42564)
                     /locus_tag="IVY85_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8043583.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(42634..43917)
                     /locus_tag="IVY85_000547"
     CDS             complement(42634..43917)
                     /locus_tag="IVY85_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8043584.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(43931..45616)
                     /locus_tag="IVY85_000548"
     CDS             complement(43931..45616)
                     /locus_tag="IVY85_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP8043585.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            45891..46916
                     /locus_tag="IVY85_000549"
     CDS             45891..46916
                     /locus_tag="IVY85_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8043586.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT        14674 a         9318 c         7522 g        15942 t
ORIGIN      
        1 aagcttacgc cgtctttcac tgcaaagact gttgttccac tttcaatact tgttccttcc
       61 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
      121 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      181 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      241 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      301 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      361 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      421 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      481 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      541 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      601 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      661 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      721 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      781 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      841 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      901 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      961 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
     1021 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1081 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1141 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1201 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1261 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1321 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1381 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1441 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1501 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1561 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1621 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1681 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1741 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1801 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1861 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1921 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1981 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     2041 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2101 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2161 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2221 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2281 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2341 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2401 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2461 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2521 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2581 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2641 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2701 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2761 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2821 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2881 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2941 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     3001 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3061 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3121 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3181 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3241 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3301 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3361 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3421 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3481 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3541 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3601 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3661 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3721 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3781 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3841 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3901 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3961 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     4021 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4081 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4141 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4201 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4261 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4321 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4381 aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg gttttaaaca
     4441 tgtatagaag aaacatctca ttaaaatcac cagtaaagaa atggaaatct ttagtttttt
     4501 taatattatc agttagtaac tttttataat cataaaaaaa ataagtagtt ctatctgagc
     4561 gatactcttt tataatactg aattcggaat taaaaaccac gataggtaac tttaaacaaa
     4621 catgaagaga ctttaataag acttctttat ctgaaaacac ttttgtttct ctctttcctt
     4681 tctacatcat taaatagtat tttatacgtt atctaaaata ctattcctct gtcaaaataa
     4741 gctaacttac ttcattataa aatatccact tatcgctttc atgtatattc caccgtaaaa
     4801 agtacatttt atcaccgaga actcatcatt tttcatcatg atgaatacta tttacatata
     4861 tctagttcaa agacaataat tattatgatc cgttattatc taagcactat acagataata
     4921 ggaatttttc gtttctaaat attccaattt gggaatccaa taaaaatttt cgttcaaata
     4981 cgtgtctatt cgaactcttt tctagttgtt aattatgaag ggggaagaaa taaaaaatac
     5041 aatctataca cgaataagaa tattatccaa aaggaggttt tagaaaagat ttattatacg
     5101 taaaaagctc aagacatttt cttagccata aggacatgtt tgatatttaa gaagatatat
     5161 gaattttctt ttctaagtat aaagataact atccattatc aactaaaaaa acatttttga
     5221 aaataaacat cagcagtcct atcaatgcat gtgagcttat agaagttatt cgcagcgcag
     5281 cataattgca ttagcttctt ttcgtgtttt ttgcctcttt tccctttaaa tcttaatgga
     5341 ggaggtgtgc aatatgtcag ataacgacga acgcgtaaat gcccagttga ttcgattctt
     5401 tgaaaaggtc attttgaaca ccgctaatac ttattataaa aaacaagaag aaattcaaaa
     5461 acatgaacaa cttgatcaac tcccaccata tttgtttact aaagatacta taaaatttaa
     5521 acaaccgatt aatgttttaa atgtgactct ccttgttgaa gatataaaat tagcaaaaat
     5581 ccttcctttg ttgaaagaac ctgaacaaat ttttcttatc gaaaaattta tctttgataa
     5641 aactgataaa gaaattggaa aggttctggg gattacaaga cagggtgcga caaatttaaa
     5701 acatcgactt tacaaaaaaa ttcaaaaaat gctggtttaa tatctgcata ataactaatt
     5761 ttgtatgctt ctttgctcgt tctcctcctt cccaaagaaa tagattataa gtccatggga
     5821 agaaggtaga tttatgaatt ctttaaaaga agatttcatt ttagcaaagg ctggaaatga
     5881 agaagctgtt gaggcaattc tcaaaagatt tagctctttg attcataagc aatcctggag
     5941 aactggaaaa tatgatcaag attgttatca agaatgtatg cttgcaatat atctagctat
     6001 ctcgaaattc gaaattaaag aataactcaa ctaagggagt ctgtgacata agtaaaaaga
     6061 tagtttcaaa tttcgaacaa taaatttgaa actatcttct ttataattat aaagtatcga
     6121 ttttacagcg ttctataatg attcatagaa tctaaaacga atgattttaa ttatcaacgt
     6181 tttttaattc gtttttagct taaaaataac ttttgtcaca gtctcactcc taaaattact
     6241 ttatttacgt atgaagtggg caggaggtcg aatccagaac tgggctatgg tacttcaacg
     6301 aggacttctc ctctagaatt gaaaagcatg cgatttacct tccgtaacga cggtagattt
     6361 attcaactat gtgtccaatc aaaatttgtc tgatttcaaa aatagtcaag agaattttgg
     6421 ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt gacaaaccct gatactaagt
     6481 tgggctatga tacacttttc ttgactatca gttacgttac cggaaggaat gaactctatt
     6541 gagaaaaaca tatgatcgta aatttaaact aaagatttca caggatatcc ttgagaaaaa
     6601 agtaactacc aagaaaattg ctgaagaata caatatctct cgtccaacaa tttcccgatg
     6661 ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct ggacaaggaa aaagattacc
     6721 tgataaagca gatttctaca ttcttgaaca ggaaaataaa cgactaaaag aggaagacga
     6781 gtgttctacg aacacaaaga tcgatacgga tctccaggga tcactcaaaa actttacgat
     6841 gaaggaatcg aaatcaataa acgtgttgta gctgttctta tgcgtgaaat gaatttatgc
     6901 acaagaggat ttcatcgacg caaatcttct catggtagag aaaaaacaat caaagaaatg
     6961 gtaaaaaaat gttgaatcga taatttgaac agaaccaaat tgatgctgtt tgggtgatgg
     7021 atattactta cattccatgt aatgacggtc gcctctattt gtccacctat attgacttag
     7081 caactcgtat tctacgttac tatatggtag attcacacat gaaaaaagag attgtcgtcc
     7141 gtccattaca aatttattaa ggagaattgc ctacggtaat tcattccgat agaggtagcc
     7201 agcatcggtc ttttatttat caagagctgt tgaacgacca ccacatcaca cattcagtga
     7261 gccaaactgg gactcctgtt gacaatgcag taattgaagc gtttcatcga tcaatcaaat
     7321 gagaattaat cgactccaac aaatataaaa gtaaaataga gatgaaggtg ttaatccaag
     7381 attatttaga agactactac ccaaacaaaa gaatccatac aaaatttatg atgactcctt
     7441 gacaatttga agcgaagaaa ataagggatc tataatatct agtgttggtg acgccttctg
     7501 ttgccaatac tttttctttt ttctaataaa cgcaaacaga caccttaaaa tcctttagaa
     7561 taaagtcgac gaaaaccatt caaaggagtg tttcaagatg tcctattcag agtttaccaa
     7621 agagttacta gatattctag acctaaatct tatctttcat gaagatgcct ttcgcaaaga
     7681 gcgaatcaat gacgaaacat gttttgtttt tgatggcaca ttgacctatc aaccggagga
     7741 atgctttcat cgccactatc aaaatgaaca aacgatcatt aaatagggct ggaaaaaagt
     7801 atccatctta ttgaatgatg tcagtaacta caagaccatt ctgcggatta acaaacagcg
     7861 cttcaaatgt aaacactgtg gaaagacttt tttagcagaa gattcagtta gtgatcgtcg
     7921 ctgctccatt gctcgcagag tcaaacaagc cattcttgag ctattaagcg aacctctttc
     7981 tatgtctttg atcgcacgta tgaagcacat ttctccgacc actgtcattc gaatacttcg
     8041 cagccttcgg cctaaaacca tttctttgag ccagctgtta ccagaagtgg tgtgtttcga
     8101 tgaattcaaa tcagtgaaaa atgtctcggg agccatgagc tttgtcatga tggacggcaa
     8161 gacccatcaa ctgattgata ttgtggaaaa tcggcaactc aatcctttac gcgactattt
     8221 cttgcgctac cctataaaag tccgtgaaca agtgcgtcta gtggtttcag atttctatac
     8281 accctatcgc acattggtga aagagtgttt tccgaatgcc cgaatcgtcg ccgatcgctt
     8341 tcatattagc caacacattg gtcgggcttt taccaaccac cgaattcaag taatgaagac
     8401 ctttaaaaaa ggggatcgtc ggtcgaagca cttaaagaaa tactggaaac tacttcaaaa
     8461 gaatgcgtgg gaattgaaag gtcagcatcg ctactggcgt ccgtcatttc gagatcatct
     8521 gacagaagca gagatagtcg atcggctatt atcttatgat gattccttaa agcgaggcta
     8581 cgaagtctac caggattttc tttccgttat cagaagacaa gatgttcctg aattcgtcgc
     8641 gctcctaaaa gaagactaca aggagttgcc agagcactac caaccagtgt tcacgacctt
     8701 caagaaatac caaacagaaa tcaaacgagc gttgcgcgtt ccctattcca acggccctat
     8761 agaatgtttg aataaccata tcaaagtact aaaacgaatt gcctatggct atagaaactt
     8821 tcaaaactac cgagaacgaa tctttttata tcgaggaaaa tattttaaga aaactaagaa
     8881 tactacccaa ctgactaaag ccagaacaac aacacggcta gacaagatag ctgtctagcc
     8941 gtgtgagtat ctcgattatt ctattgaatt tcttcaccaa cactcattga cgaagagcca
     9001 aaataagtaa tctaaccgac tgttaactaa accattgcag gtcccgttaa agatacttgt
     9061 gcctgttgtc accgcttctt tttcttgtcc acaagcgctt aacacactta ataaagcccc
     9121 tgctattatt aataataata tctttttctt attaaaaacc tcctcatcta tttccagatt
     9181 agtaaatgta gtaatctgtg ctttcttttt tctcaatact aataactaac ttatggggta
     9241 aacaaatact cgtttgaccg ggttttgaga tccatccagt tctaaccgcg atttgatcag
     9301 gactattatc ttctttaact cgaattcttc catctttaat ctcaacaata ttatattgtc
     9361 ccttcgcagg atgataattc acgagtttat gattgatctt atccaaatcg aagcgatcca
     9421 tttccttgcc atcaattcga acgatggcat atttgatagc atcttcagtt gtttcattct
     9481 ccttttctat agagaataca aataagggaa taaaagaggt aataatcaaa atgccaataa
     9541 ttatgacatc gcctatcttg acaccagctt ttcttaaaaa tttcattaac gatatctggc
     9601 taaacatatt agactttttc catggaaatg gtgaaatttt tcggaaatta tctgcattaa
     9661 gagaatcagt taaacaaccc tcaagttgtt ccttaataca ttgttgatcc atcttcatac
     9721 caataaaaac tagtttatta atgcgttcac caaacaacgg atgccaggct tccttcaatt
     9781 cgggattttt ttgtataatc atttgacact caattttagg taaactagct acccaataat
     9841 ttaatgattc aatactttta tagctacccg atgtttcaaa gatcgtcgaa aaatcactat
     9901 gttgggcaaa ccaaagaaac ccttttgctc ttgtaatatt gccaggaaaa tttttatccg
     9961 taagatgggc aaatttttgt gcgtcaagga gtaaccgatt tcgatagata aaacttgaga
    10021 taccatattc ttctgtttca ggaatatgat tttgatagcc aagctctaac tctttgaacc
    10081 agcccactga ataggaagtc tcatctatat taaaaagatt tgtatttaga attttcgata
    10141 agtctacttc acctttaatt gtcctaatta ttttggcatt ggactgtaac ttagcaatta
    10201 gaccgttaat tttgtaaatc gtcttggttg acactaagtc actattattt aaaagtaata
    10261 tgttacaaca ctcgaattga tcaattaaca agccacgaat atcctgttct tcttcaacta
    10321 cattaccttc agcttttctt tcctgaatct tttcacccgt ttcgaaatta tcccaaaaac
    10381 gttttacatc cactactata ataatggcac caatctctaa tcgttgggtc aaatcaatgt
    10441 ctaaagggtt tttagctaat ataattgctt gaataattgg aattggtttg ctgattcctg
    10501 aagcttcaat aacaatttga ttcaagtcag aaaattgcac taattgatct agttcaacaa
    10561 tcaaatcttc gcgtaatgta caacaaatgc atccattcgg catcgaaaca agtttttctc
    10621 cagttctttt aaaacctttc ttgtcgacta ataccccatc aacatttact tcccctatgt
    10681 catttacaat gacagcaatt ttctttccgt gatcaccgta cagaatatga tttagaagta
    10741 tcgtttttcc tgaaccaaga tagccagtta agattgtaat aggtattttt ttcataggga
    10801 acctctttct ataccttgaa tttacacttt aagtgcaaca cataaaaatg agtcataatc
    10861 gatatccaac tagtataagt ttgtcgagaa cactcctaga aggaaaataa ttatgagcca
    10921 gtatcgtcat cttactatac tcgaacgaga aagaatcttt ctgcttcacg aagaaggatt
    10981 ttctattagt ataattggca acgacattca gcatagcccg tttactattt tacatgaact
    11041 gagaagaaac aagaaaaaaa ctattcacct tccaaagcgg aaaaaaatta tgcttatcga
    11101 agaaaaaaat gcggacgtta tcacattcta aatgatccgt gagtttggtc aattgttcgt
    11161 catttatttg tcgattttca gtggtcgcca gaggaaatct cgaatcgttt gaagcatgaa
    11221 catatggact tttcaattag ttttatcacg atctaacgtg caatttgcaa tgatcttttc
    11281 gatacagaac ccctctcaca cggcaataaa ggcctgatcc gttcattgca ccatcgagga
    11341 aaaacaagac atacaaaaaa tcatgtcgag tctcgtggaa agatttgcct tactcattcg
    11401 atccatggcc gtccaaaatc cgcagatgat agaagtgatt ttggtcattg gaaagccgat
    11461 attgtagctg gcaaaactgg tggtacttgt ttagtgactt tgacagacag aaaatcatgc
    11521 ttccttttag caggaaagaa ggtccccaga aagaaagccg cttatgtgag cgagaaaatg
    11581 attgaacttt taagcagtat tccaaatgag gcggtcaaaa cgatcactcc agatagagga
    11641 aaagaattct ccaatcattt cgcagttaca gaagcattga ataatatgcc cttttatttt
    11701 ccagatcctc atgcccttgg caacaatgaa caaattaaaa tacgaataga ctcattcgag
    11761 aatacttgcc aaaatcgaaa gagatagcta cggtagataa ctcaacgata aatcagatga
    11821 ttgaaaaact gaataacaga cctagaaagt gtttagattg gagaacacct tatgaagtct
    11881 tctttgataa agtgttgcgc ttgatttgac aattcaagac ataataaaaa gcagatcaaa
    11941 tccaaataag ggaaaaatga tctactctgg tattatcaat taattggcca agctattatg
    12001 ccgttttgct aaacgtctac ttcttccagt attgaccttg ataaaacaac aatcaggatc
    12061 ccgttttaat actctatgaa ctgtgtcaat actaacatgt aaagttgcag caatatcagc
    12121 taatgtactc ccatttaatc tcatttgctt tgcaaatcga taacgccgat aagcatcaat
    12181 attcttggaa tccatagaca ataatacttt tcttaccatg tatttcctcc aaaaattgaa
    12241 ttttaatgag ccatcatttc ttgagcaatt tcatgtttag acaatgaagc tggatagaaa
    12301 gtaggccaat tatgcaattc attatagagt ttttcttgac tttctccacc aaagaaaata
    12361 tggaaatgct ctgccttact tggtgcaatt ccatgatcac taaattgaat atatttaaaa
    12421 cgaccagcac ctgcatcatc agtttcaaaa ttaaaacgaa caccacggtt tcctttttca
    12481 taattcaaaa ttttgtatcc aacatatttg tatgtgtatt gatgatgttc accgttgact
    12541 acaaaatcaa tggtgttgtc tttaatgttg atagtatcaa tatctgtctt atagccagta
    12601 gtataataat ctttgtactc ggcagctgtc atgtctttat tgatttttgc tttgtaatcg
    12661 aaaacttcat ccaaaattcc atcttttaac aacggataaa cagattgcca ttcgcctgta
    12721 taatcactca attcgcgatc cttcacatcg gcatctttaa agtacccatt agcaactgtc
    12781 ttttcatcct tcgcttcttt ttctggaact acctcttcac cagcaaccat agttgttttt
    12841 tctaacgctt ttagattgtc ttccattact gaaacataat cttcaccatt atccatttgt
    12901 tcttttgtaa gactttcaag aggattcagt acttccaatt ttatcccagc ttcgttggcc
    12961 aaagtttttg caattttatc attagcattc ttttcaaagt aaatataatt gatcttattt
    13021 tttttaacat actcttttaa ttcagctaat cgaccagaag atggctcttc ttcaggactt
    13081 agtccagcaa taggaacttg tttcaatcca taatccaaag ctaaatatcc aaaggctgcg
    13141 tgttgcgtca caaaactttt ttgtttcgct tctttaagac tagtggtata gtcagcatcc
    13201 aaccgtttca gtttagtcaa atacttttca gcatttgtct caaatacttt tgcttttttc
    13261 ggatacaatt taactaactg atccttgata tttgaaactt ccttaattgc cattttgggt
    13321 gaaacccacg tatgaggatc tagttcatga tggtgatctt cttctccgtg atcatgatct
    13381 tcttcttcac tacctggcaa taaaatcatg tcttttgttc cttctactac attaggccca
    13441 gctttcttcc aactttcttt agcttttggt acccatgatt ccatgttttc gttatgatat
    13501 acaaaaacat cagcatcatg agcttttgcc atatccttag ctgagggctc atagtcatga
    13561 ggttctgatc ccgctggaac cattaaatcc acgttcgctt catcgccgac gatgtttttg
    13621 gtgaaatcat acattggata gaatgttgtt acaatttcta attttccatc tttatcattc
    13681 gatacttttg catcattagt gctggagcaa gctcccaata atcctagagc tgctatccca
    13741 ataagtccta cactgatttt cttcattctt tttccttcct taattatgct aatttcttca
    13801 ttttatttcg tttatgttct ttgataattt ttaatgccct ttttctagtt ttaatattcg
    13861 ggcttttaag tcgtcgatat gcactagata tatccgtttt ctccattaga aattaccccc
    13921 aatcactact tatcattatt tgctttccgc cagtaattta tcaaaactct gtttgatcct
    13981 ttcttcatcc aacatttttc cgattagaat aatagtggtt tggtcttttt gtcgagtggt
    14041 gtattgaaaa ttaacctgtt cgtttacctc ttgcatggca actataaaat cctgctcttg
    14101 taaagcaatc aatcctttaa aacgatacag gctcccttgg ttggttaaaa tcagccagtc
    14161 aatccattgg agtaaaaaat ctttttttat gtcttgatct gaagttaaga gaattgattt
    14221 aaactcatgt acttgatggt gttgatgata gtatttgcca ttatagtgat tttctgatga
    14281 atcactttca aaaactaagg gatgattaaa ttttttcaaa ttataaaaat cctttttttg
    14341 aatcgtttca ctatgtgtga acattcgaat atcggataaa gggttaatac ttttgatttc
    14401 tctctgtaat ttttttagtg cagataaatt caagttttgt tttctagaca caaaaatgcg
    14461 atctgccatg acaagttgct taagggcttg tggttctctt tcaataagtt caaaactgtc
    14521 actatcaata acagtcaata aactatcgat atatactctt cctcttagtt gaggcgctgt
    14581 taaaagtgtt tgaatgattg gttggggatc tgctatacct gtagtttcaa tgatgatttg
    14641 tgaaattggt tgttgatgtt cttctattgt ttccagtaaa gaaatcaata ttgctaataa
    14701 atcactgcga agactgcaac acatacatcc attatttagt tgtaacaccc gttcctcgct
    14761 atgaactaat aatttgtgat caatcccaac ttcaccaaac tcattttcaa ttataataat
    14821 ttcttccctg gggtagggtg agacttgcaa ggcttgattg attagcgttg tttttccagc
    14881 ccctaaaaac ccagaaacga ttgtaactgg tatgcccatt ttgtcacctt tttaccactt
    14941 agctatttca cttctttaaa aattgcccgt ctgcgcaatt ttggcgaata cttttttaat
    15001 tctaaccgtt cagggttatt ccgtttattt ttgctggtga gatacagacg ttctccagtt
    15061 tcgacacatt ccaaaataat attttgacgc ataaatcttc ctccttcaat acttatcgat
    15121 cttcaagata tgcttgaatt tgttgtgcat cttcaaaagt taactcgcca gattccaatt
    15181 gtgaaactaa attttctttc atatcaacag gtatctccca ataaccatcg atacattgaa
    15241 ataactcctc gtcatactct tcaaaaattg gatagattct taaaaaatca tcaaatgata
    15301 agccttggtc ctcatagtaa tctttgacta atccattttc aattgcctgt tgggtcggta
    15361 aacccgtttc agtaataatt cctctctcta ccatcgaacg ataaaaagtg catttatcta
    15421 gcgattgctg ggtcgtttca agataatact ttaatccttc ttcaactaaa ggtgggtaaa
    15481 taatttccat tttgattccc tcctaaaagt tatatattaa actattttga agctccttta
    15541 attactttct ttccattata gtaccctttt aaagatacat gatggctacg tctataatca
    15601 ccattcgctt cgtcaaatga aatttctggt tttactacag tttgatgagt tcttcttaac
    15661 cgtttcttcg ccttagatgt ttttcttgct ggtactgcca tgatatttcc tccttttacc
    15721 aacttgcttt ttttactcca ggaatcaacc cttgatgggc aagttctcta aacttaatac
    15781 gggacatacc gaactttcgc atataccctc ttggacgacc atccgtctga tcacgaagcc
    15841 tcagacgatt cggatttgaa tctcttggca atttagcgag agcggtgcga tctcctgcgg
    15901 cttttaattc ctgacgttta tctgcatact tttcaatcat cgccatttgt ttctttgctt
    15961 tagcaatttt cgactttttt gccatatacc tttatactcc ttttattttg atttagacac
    16021 ctcaaatcgt aatcgttacg ttttgacact gatactttac caacaagagt tgctcttgtc
    16081 aacaataaat aacatgaaat aaaactcata tgaatagata actatttgaa aacgaattta
    16141 tcggtacata gagataatca aatcatttaa aagaatatgc agtttaggct ctgaaaaata
    16201 agaattcact tttattagtc caatcaaaca caaataccgt tttaagtagc ttttcctcta
    16261 cttaaaacgg caatatgaaa gaaactataa ctttttattc aatcttttat gtttaactgg
    16321 tttatcgcca attttttaaa actttgatta ttccttatat tataagaata gacattaagt
    16381 gaactaattc aatcatactg attctttcgt gtattcaaaa ttagatgctg tttaataaaa
    16441 ttattctttt tgaaaaagaa ttataactat atcccaaaaa ttgtaggtta atttaaagcc
    16501 tatttcatca tcgtgattga gcttcttttt catagacgaa tttctcagat agtttagttt
    16561 tctttatgag atttttgtat atttctgacc ttcgatacaa ctcattatga gtaccttgat
    16621 caactagttt tccttgatcc atcactacaa ttttatttac cttttcaata gacttcaagc
    16681 gatgcgatat aattagtact gtcttatctt gtattaaacg atttaaactt tcctgaatac
    16741 gtttttcatt ttccgcatct aaactggcag taatttcatc taaaattaaa attggcgcat
    16801 ttttcaaaaa agcccgtgct atggataggc gttgtcgctc tcctccagaa agtgaacttc
    16861 cgttttctcc gaccaatgta tgataaccgt caggtagttt ttcaataaag tcttcgcagt
    16921 tagctaaacg agctgctttt ttcacttcct catctgacgc ttgactattt ccaatacgta
    16981 tattttccaa aatacttgta ttaaacaaag ttacctcttg aaaaacaata gcgattttgc
    17041 taaacaaaga agctggagat acacgtttaa tatcataccc atcaattaaa atacagcctt
    17101 catcataatc atacagtcta gacactaatt tcaagatact agttttcccg gaaccactag
    17161 cacctactaa cgcagtcact tctccttgtt tagccgtaaa agagatatga tctaaaattg
    17221 gggtattgtt atcatatgag aaagagacat ctcgcaactc aatgtcaaaa gatttcagag
    17281 gactatctga tccctcttgg atactggttt ccttcatagc tctaattcgc tgtattttag
    17341 gagctaaata aaatatttca agcactgctt ctttcataga atcgaaggaa tcctttattt
    17401 ttattgcggc tagtaagtaa ccaacgacgt agagtatagt cacttcaccc gttaacagta
    17461 agtgaacacc tactagaatg actactgcta aactaacata tgagaaaata gaggataagg
    17521 ccatgactga aaaagtactt aattctacct ttaaatgaat cctttcactc tcttccattt
    17581 ttttgaaaag tctgtcttga acttttttag atagattaaa gctattaatc tcttgctgca
    17641 attctatagt ttcttgaaat tcttccgagt tctctctcaa agtatcgtaa tatttagtat
    17701 tggctttcgt ttgggatttc tttgataaca agattaagac aaaactaaat aacgttggca
    17761 aaataacagc taatcccatt ttgacattac caaccaaaag catcactgaa ataaaaggga
    17821 aaaacagagc cataccgccg gctttaggta ttgcatgact catcgcatgc tcaatacctt
    17881 caacgtcaga catgatagtt tgagataaat ctgataaatt atgttttgaa aaataggata
    17941 atggtaaatt tgacaaatcg tctgcaattt gcactcttaa atgcgcactt tctttataag
    18001 tactattata taagttttca tactccctat ctaacaacca atacatgaaa agcaaagtaa
    18061 caaacgatac ggcaaaataa agccaacggg gctttgtatt ttctaaaact aattcgtcta
    18121 tcagcatcat tataatgaat gctggtacca tattaatgca gtatacaaaa aaactagaaa
    18181 tagaagcttt agttaaagct tctgcgccct gatcacttaa agcaaatttg tttttataaa
    18241 aacttttcat tgtcaagcct ccaatcattt gcattctcat aattagtgac caattgttta
    18301 tacacttgat gtttagccat taattcatta tgatttccac gttctactat tttcccttct
    18361 gacaagacaa gaatttcatc tacagattta atgctggata atcgatgagc tatcataata
    18421 actgtttttt ctttcatcag atgttgaaaa gccttttgta gttcatactc attatctgga
    18481 tcaatagatg cactggcttc atccataata acaatctttg catcttttaa aattgctctg
    18541 gcaatagcta tacgttgttt ttctcctcct gacagataaa ctcctttact accaattaag
    18601 gtatgctctc tttcgggaaa tttttctaaa atcgattcgc aaccggctaa tcttagcgcg
    18661 tcaaatactt tacttgctgg cgcttgagga tttgctatag aaacgttatc gtaaatactc
    18721 tgattgaata gtttcgtatc ttgaaagaca aaagcaattg tgttaataat tgtgtctttt
    18781 gaatagtgat ttaaaggctt accaccaatt ttaatctgtc ccttatccac attgtagaaa
    18841 cctgaaatta gtttagctat cgtactcttt ccgcttcctg attttcctac taatgcataa
    18901 attctttttt gttctaaaac taagttgaaa ttttctaaaa cacactcttc tttgtatgaa
    18961 aagctgacat tttcaaatgc aatgtcacaa tttttaatct gtgtctcgtt cccatatttt
    19021 agttggtctt gcttcatttc taaataaagg gagtctagtg tttcgactgc atatcttgct
    19081 tcaaacaagt gcatacttac atacattatt ctcatgaaag aaacaaaaat aattccactt
    19141 aaaaaaaaca acatcaacaa gtcaacagct aatatcttgg gagtggtaag agaagttata
    19201 aaaaaggtta taggaactat caaaatgcaa actatgccaa aaaatagcca ttgaaaaaga
    19261 acaaaaggtg ttttcccact caaagaatac ttatatgcaa acttagcata atcgttaatt
    19321 gctttattca aggctttaaa tgatgttact ttagcaccaa atatctttat aacttgaatt
    19381 ccgcggatat attctaccgt ttcagagctt aacacatcta acgattcttg atatttttga
    19441 ataaaatttg tattctctgt cattttcttt aacaagaata caccaattac taacatggcg
    19501 attagaacca atccaatttt aaagttaatg aaaaagccta aggcaactga taaaacaggt
    19561 gttactatgg cttgagcact atctggaatc aagtgagcaa ttgccatatg tgttttagcc
    19621 gcgttgtcat caattgtctt tcgaatatat cccgaagaat ttaaatcaaa aaaacgaaaa
    19681 cttgcttctg ttaaaccgct aattcccttt tttctcaaat tagtctctaa gcgaaaccca
    19741 aggatatgag aaaataagcc agaaaaaata taaaataaag ctcctaaagt taaccaagca
    19801 acagtttgaa ccgcataact cttagctaaa tattcatctc ctacaataat tagagactga
    19861 agaaatttgt aaatactgta atatccatag actgttaata cgacagaaaa atcagaaaat
    19921 aaagtggcta accatccgta gtatttcatt ttagggacat aactaaataa ttttttgtat
    19981 actttcattt tttcctcccc cgcattcaat ttcttctctt attcgtttac aactgttaat
    20041 gctttccgta atagcgtaaa ttagagatgt acaaacagat aaaaggtaaa ctacgttcta
    20101 ttttttgtat ttttttaaga cttttcttcc aatcaataca ccaatcatag ataaacttgc
    20161 tacaactaac actgaaatga ttccccatat acctaccgca tagtggatca ttgcatccat
    20221 tgttgcagca gattgtcctt tatctatcca atcttgacga aaacgatctg taaagaacca
    20281 tataggaatc cactgtccgg cagcactgcc taattgcatt agagcccaag acgcaacttg
    20341 aggaattgct cgatcatatc caaatacaga tagaataata tcagctaaga tcgccatact
    20401 aatcgaactg ataaaccaag gtaagtacgc cccgcccatt aacgtaaaaa ataacaataa
    20461 aacaattgtg taaatagtaa aaattccttt tctagggtag ttatagctaa tataaacaaa
    20521 tatgattcca gataataatc caaaaatccc gggggatatg ctgtgaccaa acgccccacc
    20581 aatactacta accattattg aaaaaaagta aattactagg gaaataactg tagctaaacc
    20641 aataaaaata taatcttttg ttttcatata tattctcctc aattctattt cgttatgtca
    20701 ttaaaaatct gttctatcaa agttgaatct tctactctaa gttttatatc tttttttatt
    20761 ccaccatttt ttaaataaat aattctattg catgttttat aaagaaattc cggatcatga
    20821 gtagctataa ctactgcacc cttttctttt aagtctttaa taaggtcagc aatcctttcc
    20881 atattaatat aatccaaccc acttgtaggt tcatcaaata gaaataaatt agtttttgac
    20941 aatgaagcta tagaaaccaa taatctttgt ttttctccac cggataaatc aaacggtaat
    21001 tgatctttca ttttataaag ttccattttt tttaaaattt tttcttgctg atctttacta
    21061 actatttcat tccccagggc taactcgctc ctcactgatt ctgtgaaaaa ttggtaatcc
    21121 atttcttgca ttactaaaaa cggcgatgac aaattttttt tattttcgat tatctttcct
    21181 ttggtagggc gaataatctt catgagtatt cttagtaagg ttgttttccc cacaccgttt
    21241 ttccctacca aaccaataac atctccaact ttgacatcaa aatttatgtc ttttagaata
    21301 ttttcataag taatttcttc aacttttaat acatctgcac ttaccttttc gatataggga
    21361 acttcaatcg aactattaaa aatgtcaaat ttccttaatg cgttattgtt ttcagttttt
    21421 aacaactgaa tagttgtagt ttttacagtt ctatttttaa caaatagaac ttgatcaatt
    21481 aattctttca aatagtaaaa acgatgttca gctataacaa tagtaaatcc tttatttttt
    21541 aaatcacaaa gtaagtcagc aagtttctta gtcgctttga agtccaaatt tgaagacggt
    21601 tcatccaata taattaattt agagtttagc gataaactac atccaataga aattttctgc
    21661 cgttcaccac ttgatagcga aaaaatgttt ttatctaata tatttgaaag atctaataat
    21721 tcagctaatt cattgattct tttatccata tgctcttttg taaatccaaa gttttccatc
    21781 gaaaatacta actcactcgt tgtatttgtt gtaaagaatt gtcctctagg atcttgactt
    21841 acgtagccaa tattttttga tatactttct tgtgttaagg attgtagcga gttattttca
    21901 aataaaattt ccccaaccaa ttttccgtca taaagtcttg gaataagtcc attcattaac
    21961 tttaatagtg tggatttccc actcccagaa gaaccagtaa tcaatgtaag actgcctttt
    22021 tttattgaaa aattaaggtc aacaagcgct tgttccttaa catccttata tgaaaaagaa
    22081 acattgttaa attcaaacaa ttctttcacc tcaaatcaaa atacttaaga aaaaaatagt
    22141 aacggaaaac aggatgatat agtctaataa gctcatgttt gttttatagt aagaacttct
    22201 tttcacagga gattctatcc cctttgttat tgcattagcg gtcatttctt cagcaatcat
    22261 agttgcacgt ataagaacgg gaactaacag aaattccaaa tattgcagag gctgaaaaaa
    22321 agacaagcga atgtttctca tttttaaaga ttctttaatc attcttattt cagaacgata
    22381 agtcggaaag aatctgatca ctactgtaat acttaacatt atttttttcg gtaaatgtat
    22441 tttttctaaa gaggataata attcattgct agtatagaca ctaaccaaca accatgagat
    22501 cattacgacc ggcaacattc gaaatacaat atataaggat gtcgcaaaaa atatagtaaa
    22561 aaactctatc ttgatagcat caataatttt cattactaaa aacagaactg aataaatcaa
    22621 gatgaaataa atacatgtct ttattttacg aaacatcaat actaaaactg tagcaataat
    22681 gaaacacccc acttgaaata tatttccctt tcctaaaaaa ataatgaccg gaaaaaaaat
    22741 atttaaagta atcaaggtta acggattgat ttttgacact ctttcacctc caaaaaataa
    22801 gatcgcactg ttatcttaag ctgataaaaa tgcaccgtta ttcagataac agtgcgatct
    22861 tatcaaaaag cataactgag aacaattctc aatgcaagct ttttttaatt aactcagtaa
    22921 ttcaatccac ccaggaacag aaaattgata caatcttctg gttaatagca tagtcttttc
    22981 ttttgaatag ctatgcttca atatatcata gaatgtagcg tagaatgagt aaatgtagaa
    23041 gtggatttct tcttcattaa agactaaatt ttgattgatc attccgatag tctttaactt
    23101 atctagacaa cagactgtct ttttcgtcac aaatgatgta attttttcaa caatatcttg
    23161 atatttggtt ccagctgatt ggaataaaat caaatcgaat atacgtttat tctcaaaaat
    23221 ataattaata aatggatcta attcattgaa gttctctctc caacttttct ctatatctat
    23281 attttctaag tttaagaggt atttggtaaa ctcttcatta tacatattaa taagcgcatt
    23341 aatatgcggc tgaataattt cttcaaataa cgcttcctta gaagtaaaat gtttataaaa
    23401 agcacccgta gttacgcctg cggtcaggca cagttgcctt atcttagttt gctgaaatcc
    23461 ttctttttca aaatggctta gtccactttc tattaacttt tggtgagtta taatataatc
    23521 tctggtttct atgggtactt cctcctaata agatgaatta actttattat cttacacagg
    23581 acttgaagtt tcaatcgtta acgtttatta ctgaactcat tccttagagc taaactaatg
    23641 agaaagagca gaataactga tacaaaaagc aatcctacaa gaattaatag actcatctaa
    23701 atcattcacc ttttctaaaa taatccgaga aagatttaaa aaaattatat ccagctatta
    23761 ggataagtcc tatcataatc actccaatag ccattttctt atcctcccta aaaataaagt
    23821 cttttactta aaaccttttt tgtaatcgtc aataaatttt ttccctttat cagttaattg
    23881 aaatcctata tctaaatagt cctcatatct aagtttagcc agcatctcac ttacagatgc
    23941 tggactaaca cttagaactt ttgctaaatc attatttttt aatttctttt ttctttttga
    24001 taattcctga attgctagta aataaattag tatagtagtc gttttaattt gtaccatacc
    24061 taccctccaa attatttcga aaggccttca gcaattttat ccaggttcca tttcatcatc
    24121 gcatagtaac tatcaccatc ttgtcctttt tctgcaattg aatcagtaaa aatcgttgaa
    24181 tagataggaa tattggtatc ttttgatact gttttcattg gtctatcatc cacactgctt
    24241 tctacgaata aggagggaac ttttgttgtg cgtaattttt caactaagtg ttttatttga
    24301 tctggtgttc cttcttcttc agtgttgatt tcccaaatgt aagcagaagg cacattatac
    24361 gctttcgaga aatatttaaa gcatccttca cttgtaacaa tcattttttt atcattcgga
    24421 attgaagcaa atttagattt agcttctttg tctagagaat ccaatttttc aatatactta
    24481 tctagatttt ctttatagaa ttttttatta tcaggatctt tttccgctaa ctgtttttca
    24541 atatttttag cgtaaataat accgttttct aaatttaacc aagcatgagg atcttccttc
    24601 cctttctcac tctgaccctc taagtaaata acatctatgc catcacttgc tgcaaaatag
    24661 tctttgtttt cctctttgtt cgcattttta actaattttg taaaccaagc atttcctcca
    24721 gtttccaagt taacaccgtt ataaaaaatc aaatctgctt ttgaagtttt ttgaacatct
    24781 tcaggcaaag gttcatattc gtggggatct ttcccaatag gtacgatact gtgtaaatca
    24841 attttatctt ttgctatatt ttcggtaata tctgctaaaa tcgaattagt aactactact
    24901 tttaattttt cgttcgaagc cactttatct ttgtctttac tatttattga acatgccccg
    24961 aatataaaaa gagcaatcaa aatccccaga cttcccagta ctgctttcca ttttctcatc
    25021 ataacgatct ctccttcttt cttaaaaatt tttgttttgg tgagattaga aaactaatta
    25081 caaataagaa tgccgctgtt aaaacaatac ttgatcctgc tgctatgtta aaactataac
    25141 caataaacaa tccaagcaat gatgaacttg ctccgactgt tgcagaaata aatatcatag
    25201 ttttcaagct atttgcatat aaatatgctg tagctgctgg tgtaattaac attgcaacaa
    25261 ttaatatagt tcccacactt tgcatagctg ttactgctac taaagttaat agaaccatca
    25321 atagataatg ataaaaactg acattcatcc ctattgcttt tgccattaat ggatcaaaag
    25381 aagtgattaa taattcttta aaaaatagaa ctactataac caacactaaa accgaaacgc
    25441 caattgtaat ccacttatca atatcttgta cagctaaaat atttccaaat aaaatatgaa
    25501 ataaatccgt tgagctattt gcaactccta taaggataac tcctagtgct aaaaaagaac
    25561 tgaaagtaat accaattgct gtatctcctt ttattacact attgcttttt ataaaagtaa
    25621 taatgataga agctaataga ccaaatacaa tcgcaccaat aaaaaaatta atacctaaaa
    25681 tataagaaat tgctactcct ggcaaaacag catgagaaat agcatcaccc ataagagaca
    25741 ttcctcttaa aataataaaa caacctactg ttccagaaac aatccctata actacagatg
    25801 taattagagc attctgtaaa aagtgaaagt catacagacc attaataaaa ttttgaagca
    25861 tattattccc cctctccaat aaatatagta tctccatagg ctttttttaa attttcttca
    25921 ttaaatacag attctacact tccgtgagca attagatttc gattcaaaag aataatatta
    25981 tcaaaatact tctttacctt gcttaaatca tgatgcacaa ttagcactgt ttttccctga
    26041 tgtttcaatt tttttaacgt atccataatt attcgttcac taactaagtc tatccctaca
    26101 aatggttcat ctaaaaatat gaaatccgca ttttgagcaa gacatcttgc taaaagtaca
    26161 cgttgaaatt gtcctccaga taattctcct atttgatgat tagaatattt tcccatatcg
    26221 accttttcca aagcctttga aactctttgc cattctgcat ttttaattct ttgaaaaact
    26281 ttcattccgg catatgttcc catagataca cattctttta cttttattgg aaaagtaaaa
    26341 tcaatatcag ctttttgctc tacatatgca atcttcacca gttttttatt gatatcttca
    26401 ttattaatgg agacctctcc ctgatgaggg ataatattta acattgcttt aagcaatgtg
    26461 gattttccag caccatttgg accaataata cctgttaaag caccttcttg tatttttaaa
    26521 gaaatctcgt tcaatgctaa aaaatcgtta taacaaacat taagcttttt cacttctaac
    26581 ataactttct ccttctaaat gataatgatt atcacgatca tatggtatac aaaaattata
    26641 ttttagtcaa ccctaaattt tatagtttag atttttttct gttgctgaag attagaatga
    26701 caaaactatt tgaaactacg cgtatagtaa gtagctttca aaatatataa aaaaggagcg
    26761 ttctcaaatg agaacatgct cctaaaacta ctttattggc gtatggaaaa attatacttg
    26821 aagtggatag cgggttgaat aagttgataa gcatcaaaac atttataaaa attattcaag
    26881 actatgtctg ttccatatac aaatatgaaa atcagtaaac tccatttaac ttttttcgta
    26941 gtgtgtcttt ttatacatgt ttatcgttat taatagtgaa taggaaaaaa actatttttg
    27001 atcattgaca attaaataca atttttgaat aacgatccct gctattcgag ctagaaactc
    27061 aagtacgcca tgacctgatt agcgacaaat acttagagat ttctttttgt tctgggaaaa
    27121 caaaatgcca tgacaatagc agcaaataat gatacttccg taaggctcgc cgtaaactgg
    27181 ggagaggtga aattcctatg tgcttaccca aagcaattca aaaatttcat aactactatt
    27241 ttaggagatg attttataga atccagagtc tacctgaata aaaaacaaac ctgtgcattt
    27301 ctaggaatcg ataataatac tctagataag tggattcgta tatataattt cccagtaata
    27361 agagttggtg gtgtagttcg ctttgacgta gaagatatta aagactttat gaatacattt
    27421 aaaataactt gaaattttct tctcgccgca tatactaaaa ttagtgaaat atactgcctt
    27481 acttttttta aggagtgagg attttgccta caaagctttc aaatgggaaa tataaaacta
    27541 atctgagata tccaaaaagg tttaaagaaa taacaggaat tgcatcagaa aagtatcaaa
    27601 aaacatttcc aaataggcaa ttggcgatta aagccgaaaa tgatatgaag aagaaaatag
    27661 aaaaagtttt gagagaggag aatgcaaact cattagaatt aaaaggaaaa ataacgttta
    27721 aaaaatttta cgaatctaaa tggcttccta gatatgaatt aggtcaaaca attagatcaa
    27781 acaggcctcc tagtgatata accattagta atacaaaaga tatatttcgg ttgcatattt
    27841 tacctatgtt tggtgaatac gcaatgaatt atctaaatat caatactgag ataatatcag
    27901 atgaattaac caaaaaatcg aaagaatatg caaatataaa aatcataaaa ggatatgttc
    27961 gttccatgtt cgatattgca gaaatactaa attatatcga atttaatcga actactaaaa
    28021 ttattcaaag tatcactgca cctaaaaaaa atgctttaga agaaaaaaga atacaagaag
    28081 gtaagcaagc actatcatca aaagaactta ctaattggat agaagcagta aatgatgatt
    28141 ttaataatca tttacttact tttcatgatt acacactttt tatgattacc ctgtatcttg
    28201 gtgatagaaa aagtgagact tacgctttgc aatggaaata tattgatttt gaaaaacaaa
    28261 cggtccgtct taaacatact ttagataaat atcaaagaaa aaagtttaca aaaggtcgaa
    28321 aagataccgt cattcaagta ccagaagttg taatgacatt gctaagtgaa tggaaatcgg
    28381 tacaagctga tcaattgttg aaattaaaaa ttaagcaaac gttagatcag tatctcttta
    28441 cttatactaa accatctgga gaagtaaatt gtcctgttca tgccgattac ctaaactatc
    28501 gaatcaattc aattaaaaga aggcatcctg acttagttca tttatctcca cacaaactcc
    28561 gtcatactta tgcaactata gctagacaag gtggagcaga tatgaatcaa atttcaaatg
    28621 ctttaacaca ctcagatatt tctacaacaa agatatatgt gaacacacca gatatagttg
    28681 ataaggctgt ttttgaagct tttcagagag gattgaataa gtgtgattga tatgtgttct
    28741 taaatgtgtt cttaaaaaca aaagaagcac tttccaaata attgaaaagt gccagaaacg
    28801 ttgatatata aaggatatta acgttttgag aattgtgaag ctttacgtgc tttcttaaga
    28861 cctggttttt tacgttcgac catacgtgcg tcacgagtaa gtaagccagc acgtttaaga
    28921 gctaaacgga agtcaggatc aacttctagc aatgcacgag cgataccatg acggatagct
    28981 cctgattgtc cagcgtatcc tccgccgttt acgttaacaa atacgtcata tgcgcctttt
    29041 gtttcagtaa cagcgaaagg ttgattgatt acttcacgca agtcagcatg tggaatgtat
    29101 tcttcaacat cttttttatt tacagtaatt ttgccagttc ctggtactag acgtacgcga
    29161 gcaactgcgt ttttacgacg gcctgtgccg atatattgaa cttgtgccaa tgaaattccc
    29221 tcctattaga ttaagtttgt gatgtctaaa acttctggtt gttgtgctgc atgtggatgt
    29281 tctgctccac cgtatacgtt caattttgta aattgtttgc ggcctaaagt gtttttagga
    29341 agcatacctt ttacagaagt ttcgatcaaa cgacgagaat ttttagcacg caattcgcca
    29401 gcagtgattg atttcaatcc tcctgggtag ttgctgtgac ggtaataaat tttgtcagtc
    29461 gcttttttac ctgttaattt cactttgtct gcattgatga cgatgacgaa atcgccagta
    29521 tccaaatgag gtgtgaaagt tggtttattt ttaccacgta gtacagatgc aacaactgtt
    29581 gaaagacgtc ccaatggaac atcagttgcg tctactacat accatttacg ttctacttcg
    29641 cctggtttgg ccatatatgt tgtacgcacg ttattttcct ccaatttaag ttctgatgtt
    29701 tgacatacac ttcgagtttc cggggctctt tgtggggcaa acaataccat ttaacatatt
    29761 acccgctaat ctagtcaatg tcaacgtttt tctgcttgat tgaaaaattt atatgaaact
    29821 ttacttcttt tctttctttt atataaaagg gtcgtttcct gtcaaaatga aaaacaaaaa
    29881 gaggctgtga caaaagtctt gtcacaaccc cttattttga ataaacggtg ttctcctgcc
    29941 aactcctcgg tcacaagtca caatatccat aaaacataag aagctgtttt ttaaaattgt
    30001 ttcttgtgac tcggagctga acggcaggct tacaacctct tggtaaacgg tgttcaatca
    30061 gctgaccctc ggtattagct caaaaaacgg caaaatatga ggaactattt tgacgtttgt
    30121 ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttttcggtc aagttcaaca
    30181 acagttttta cgcgtcgatc tctggcgctt tgcccaaatg agcttggatc atccagatac
    30241 gtttttctgt ggctgttttg aattcgataa agatatcttg tgttggatcg tcgccttctt
    30301 ctccagaaac ttcgattcct ttttggtaaa gatctgctag ataacgatag cctgcaacaa
    30361 gtgtttccaa acgttctgga atagtacgat cccagcggcc aatttcatca gggatttttg
    30421 tgttatctgc aaattcacgc aatgtagaat aaggtgaacc gtctaatgtg atcaagcgtt
    30481 cggatacttc atctaactga gcgttcaaat catccatgaa ctcatccatc attgggtgca
    30541 atgtaaggaa acctggtcct ctcatgtacc aatgtgtttg gtgaacaaca acagacattt
    30601 ggcttagatc tgcaactaat tgatttaata cttctttggt ttgttggaat ttcatctttc
    30661 ttgcctccta acattttctt acacttaaaa taatagtaga ctttcaatga aaagtacaac
    30721 aaaatcattt acctgagaac gattttcagt tcgctttttt atccgtaaag ccaatcattt
    30781 ttcagaatca gctaattctt ctcgcagttg agcaaattgc tgataagtct gtgctaaatg
    30841 cttttgtgtc gaaacaaata atggatagat tttctgatat tttttatgat cagtttcttg
    30901 tactccaact tgctctttca attctatcaa ttcctgtgct tcactgatac tttggatctt
    30961 tccgatgctt ctccagccta aaatcacagc tcctaatgcg gatgcttctg tgacagtagg
    31021 gaaaacaagc gggtaaccca gtacatcagc catcatctga cgaaaaatcg gagattgagc
    31081 aaacccgcct gtagcacgga tctctgtgag ctgattttcc tggcaaactc cttttaaaat
    31141 ccgataaagg ttgagacaga tcccttctag aatcgccttc atcatttctt tttctgtatg
    31201 ttgccgctgt aaaccgataa aactgccaaa cgcatctgcg tcccaaagcg gtgctcgctc
    31261 accaagtaaa tagggatgaa aaatcagacc attggcaccc gctggcacaa aagcgatagc
    31321 cgaaagcagt gcatcatatg gattttctcc tttttcttta gcagattgaa cgacttcctg
    31381 taaaaatgta tgccctgccc aatcaaagat gccagcacca ttgctagtcg ctcctcccac
    31441 tacccaatgt ttctgatcca gaacataaca aaacgtctcc atttctggat ggaactgagg
    31501 ctgagtagta ataaaacgaa gtgctccgct cgttcctacg gtcaaagcgg cgatcgtatc
    31561 accaaaagca cctaacccta gattagaaag cggcccgtct gcccctccca aaacaaatga
    31621 tatatcattt ggcaaaccta gttctgctgc tgtttttgat ggtagacctg ttacttgata
    31681 ggtggaagga aagacttgcg gcaattgctc tattcgaatg ttcaagtagt caagcacttc
    31741 ttctgcccat tggaattcat ggatatcaaa atatccagtt cctgaagctg ttccgtagtc
    31801 gctgatcagt tcgccaaaca atctataaaa gatatagtct ttgataccga caaaataagc
    31861 agtttttttg aataattcag gttggctgtt ttgcagccac ttgattttgt tcaatggtgt
    31921 cattggatga atcggcgtcc cagtctgccg ataaaattct ttgccaagca cggtatattt
    31981 cagtttttct gcttcgtccg ctgctcggtt atctgcccaa gtgatcattc gagataacgg
    32041 tgttttctct tcatccaaca atatcaggcc atgcatcgca gaggagaagg agacaaattg
    32101 aatgattgtg tctgaaggca actgtttaat tacttctttt ataacggtgc agacaccttg
    32161 aaagatcaat tcctgatctt gttctgccat cccttgtgtt tcttggatca aagggtagcg
    32221 aaaataagcg gatgctgctt cttgggcttc atctgtgaaa gcaactgctt tggtttgtgt
    32281 ggttccgata tctaatccga ttgaaacgtt cattttttca cctcaaattt tatgtatgta
    32341 caaaaattta gtttaagctt gcgtatcttg gttttgaaaa gggggagatg gcgtgttact
    32401 ttattttatc attggttgct gtatcggatc atttttatgt ttaaccgccc agcggattcc
    32461 gttaggtcac tctattattt atcctagatc tcattgtgtc aaatgctgtc gttcgctttc
    32521 ttggtatgaa ttgattccga ttctctcgat catcgtccaa cgatttcgct gccgctattg
    32581 caggtgtcgg ttgcccttct actatttgtt ggcagaagcc ttatgtggtg gtctttttgc
    32641 ctggttcttt actttttctt ccgcaagaaa tttttcgacg ttcatatgga tgctttcggc
    32701 tttgctattt tcgttgatgg atttatttta cttcatggtc cattctcaca cgctattctg
    32761 ttcttgggca attttatgga ttttttggtt gcaggccggc gtcttccaat ggcaaagtgt
    32821 gctgttaacg tttatcgtag gcggtgtgtg ccttcgctat ggtcaatcct atctcggagc
    32881 tggcgatact ctgttgatgc tttcttggag tggtggcttg tctctagaag agttgcttca
    32941 gatcttgttt cttgctagtc tgtttggaat atccgttttt tctgtttact ttatctgtca
    33001 taaaaaaaag ttggaaaagc tgcctttcat tccttttttg agcagtgcgt tgctgattgt
    33061 ccttcatctt aggtattgaa aaaaggttgt gacaaacgac ttgtcacaac ctctgatata
    33121 tagtgttctc ttaccaaatc cttgtgactt ggagctaggc gacaggttca caacctctcc
    33181 actaaatggt gttcaatcag ctgaccctca gtattagctc aaaatgctgg ttttacaacc
    33241 tctgatatac ggtgttccat aagtagcttt ttcggcttct tatttcttga cgccccaatg
    33301 tcgacgaagg aaacctgcac gtccagaacc ttctcgataa agattgtaat ggagcttgtt
    33361 tttcttgtaa taatcctgat ggtaatcttc tgcaggataa aattcgcttc ttggttcgat
    33421 ttttgtcacg attggcttat cgaatcgccc gctgtcttgc aatgcttgtt ttgaacgctc
    33481 tgcaatattt cgttcttctt catcattata gtagatcact ggacgataag aatcaccgcg
    33541 atcagcgaac tgtcctaatg cgtcagtcgg atcggtttgc tgccaataga tttccaccaa
    33601 ctgatcataa gaaataactt ctggatcata ttcgatctct actgcttctg tatgccctgt
    33661 tgtgcctgtt gttacttgtt cataagttgg atttggtaca tgcccgccag tataaccgga
    33721 tgtcacagaa atgatccctg gctgctcttc aaatggtttg accatacacc aaaagcatcc
    33781 gcctgcgaaa actgcttttt ttgtcatata aaatccccct tttttctcat cataccttga
    33841 tttccaaaag gaggataacg ttttgcttgt gacagaaaaa acggaaacta ttatatacgt
    33901 gatacggctt ctgataaatg ccaaaacgaa cgtacttgat caaaaacggt taaatcagga
    33961 atcacatatt cagcagaagg aagatttatg ttttcaccta ttcccaatac atgcatgtta
    34021 ccagccaaag ctgcttgaca accagcttct gaatcttcaa tgaccaagca tgcatttggc
    34081 ggtacattca attgctgtgc tcctttgaga aaaacttctg gatcaggttt tgcttttgat
    34141 acttgcgtgc cgtcaataag gacatcaaaa taacttgtca aattcgtttt ttctaaaatg
    34201 aggcgtgcat ttttgcttgc tgatcccaat ccgatcttga tgtttgtttt tttcaagtac
    34261 tcaagaacag ctattgcacc aggcaaaacg gatgtttcat ccattttttg aatcgcttga
    34321 agatatagat tgtttttttg ctcagcaaag gctgatttct ctttttctga gaatttctgt
    34381 tccatttttc caaatgacag caatcgctct agtgactcgg ttctggatat tcctttcagt
    34441 tgctcgtttt ctttctcagt gaatggaatg gacagctggt tagctaaaac cagccacgct
    34501 tcgtaatgat aacgagctgt gtctacaaga acgccatcta aatcgaatag tgctgctttg
    34561 attttcatcg attacctcct tgaaaatgaa cgacttgaag taaagatgcg atgtttttag
    34621 ctgtgcgatg aagattatca gtggcttgct gtaaagcttc ttgtaaaggc acagctgcag
    34681 gaaggatcgg aaaaacggcc gaaatgccaa catctgctgc tttttcgctt cctttaccaa
    34741 cacttccgca aatagctatc acaggaacgc cctctggtac tcttctagca atcccaactg
    34801 gcgcttttcc ttttagactt tgtccatcta gacgtccttc gcctacgatt acgaggtctg
    34861 cttgctgtac ccgtttatcg aactccagta aatccaatac gtagtcgatg ccagagacta
    34921 ttttccctga agcaaatgct aacagtcctg cacccattcc accaccagca ctactgcccg
    34981 atgcctcgat tacttgaggg gcaaatctca tataaaaatc atgcatggct tggtcagcca
    35041 ctgctaattc attttctttc aagccttttt gtggtccgaa aacaaaagaa gcgccttctt
    35101 ttccgcataa aagattagta acatcagtaa tgacagtgat tttggcttta atcagctcaa
    35161 taggcacgtt cgttttatca actgtagcaa ttttacctag attttctccc agtgcttcta
    35221 ctttttgatt atcttgatcg taaaaagcat aacctagacc ttctgccatg ccgatgccgc
    35281 catcgttggt actacttccg ccaacgccga tgatgatgtg tcgaacacct tgttgcaaca
    35341 attgtcgtat cagttctcct actcctcgcg tggataacct aagtggctgt tgttggttta
    35401 gtgggacatc tttcaatccg acgatttctg ccatttcaaa aatcgcagtt tgaccgtctg
    35461 aagcgtactg gatttctttg tttttgccaa atgcattagt aacgactgct gtttgccgct
    35521 tcaaccttaa actgtctgct aatgatgaaa ccgttccttc tcctccatcg cctataggta
    35581 gcagatcata ttcagcctga acaaaaactt cttgaaaacc tgattgaatg gcttgtgctg
    35641 cctctaaggc tgtcagactt tctttgaaag aatctggcgc aatcagaata cgcattattt
    35701 tcccctccgt tccaagatca gttctttttc cagctggtat tggtctccat tgacaaaaat
    35761 aggtaatgtt tctttcgtct ctttttttat tactatttgt ttttttgtca aaacaaatag
    35821 caacctgtga tttttccaaa taaaaggaaa agaaattttt tcccatgctg aaggaagtct
    35881 tggagaaata gaaagagttc cattcgttac tgtaatacct gaaaaaccaa atataactgc
    35941 tagccatagt gctcctaatg aagcagcatg aattccgtca tctgatgaat gcggatctgg
    36001 tcctaaatca atcaggcacg cttcttggaa taaatgatac gcccattcca tttcttggat
    36061 acggcaagcc tctatggcgt gaatcgcttt actcaacgat gagtcgtgga ttgttttgtt
    36121 ttcatagtaa tgcaaattct tagaaacgat tggttgagaa aaatactcag gtaataagta
    36181 taacagcatg acaagatctg cttgcttgct gacttggagt tcatttactt cgctacgggc
    36241 ataatcaagt aaaatagact ggctgccagg acgttctttg taagtggtca aatcaataac
    36301 tggcttactg agatacgtgt catcttgcgg aatcagctgt tctttgttag gcactggcaa
    36361 atataactta tctaaaaaat tttgacattc tttcaaaaaa agcggatcgc cagcttgata
    36421 tttaacagct aaggccacat tataatgcgc cagatagttc gtatatgcat tattgtcgat
    36481 atattctgtg tattcatctg gaccaatgac tctgtgtatc tctaaatgat catcgacttt
    36541 tacagctctg ctcagccaaa aagctgctgt ttcatgaatg agcgtttttc cctttgtttc
    36601 taaaaacgat tgatcctctg ttgcttggac atactcttga acggcataag caaggtctgc
    36661 actgatatga tgttctgcgg cagctgaagc aacttcttgt cttgttccgg ttttgatatt
    36721 caatgcagca aatttgggtg tctcttcctg accagtgtgt gcgctttccc atgggaaaag
    36781 tgcaccttga tagtgataat ctcttgcttt ttgccgtgcg ccttctaatc gatcataacg
    36841 gtacagcagc aaattctttg ctacttcagg aaactgatat aaaaagaaag gcaggataaa
    36901 aatttccgta tcccagaaaa catgcccttt ataaccttcg ccagttagtc ccttcgcccc
    36961 tacactaaca cttggatcat tgcggggagt cataatctgt aaatggtaag cagcgaagtc
    37021 catcgctaac tgatcgaaag aagaatcgcc ttggatcgtt atccgatgct gatgccaaaa
    37081 atctgtccaa taagacatcg actgctctaa aatttctgta taactaacgc ttttctgggc
    37141 ttgggcaata cttttcttaa acgggtctgt ttgctgaaga gaggtagaga gcacgatgat
    37201 cttctccagc gtaaaggacg tattttttct gatcttttct gaaaaggaag cggtgatccg
    37261 gcgatttttt gcttggaata cacctgcttt attgaattta gctgcaatcc caatatcaat
    37321 tttactttct gttgtctggt aggatgcact gatcaaatcg tcaccaaata ctcgcagttc
    37381 tttttcaacc aactgctgtg taccaaaatt ggtttgctgt ccatcgatac ccgttttcac
    37441 atctacttgg cagtcttcgg tcaacggctt gatgaccact ttcagggctg cctgatggag
    37501 attattttga tcagcaacac gacaagtaat gacttccact ttttttcctt gagcagtttc
    37561 aatgagcgca tagcgtttaa gctctcccat ttctaaatct agtgcccgct cgtatgcaag
    37621 aactttcatc gtctcaagag aaaaatgttc accgtcgatt tttatttccc atctgatgac
    37681 atctgggaga ttcactaact cggctgattc tgctcctaaa ggtcggttat atacaccagc
    37741 tgcaaacatt ccccttactt gattccgata gtcttcctcc tggcttcctc tgataccaaa
    37801 aaatccattt ccaatggtca tctgactcgc atacttattg atatactcta aatcaaactg
    37861 tttttcttct actttcataa ggtaataact tctcctgttt ctgccgaacg atataaattt
    37921 tccaccagat tctggatgac atatccttgc cagccatctg caatcatctg agtctggtca
    37981 ccgagcgccc gattcacaaa atgagccata cttagttcat gtgggtcatt aactggcgtg
    38041 gtcttttgta aggaaaccag ctttccatct acatcttcga atagttcgcc tggatacaga
    38101 ttagcacctg cctgatcgcc acaaaaagcc acattcagtt tgtcttctct gatattcaaa
    38161 gcaaacgaag tattcagttg gatgatcttg ccgttttcta attccagtgt gccaaaaatt
    38221 gcatcttcta ctgtgaattt gcttggatcc catttcccga atttccctga attttttttc
    38281 gtaccaattt tttggaagct gtacgcccga acgcttttta ctttaggaaa tccagtaata
    38341 tacatcgctg tatcaagcat gtgaatgcca taatcgatca aaggcccgcc tccttgaagt
    38401 tctttgtcga taaacgttcc ccagcctgga acaccagacc gacgaagcgc attcgcttct
    38461 acgtaataaa tatccccgag aacatcaaga tgattcttga tcattgttgc ttcgtcagaa
    38521 aaacgatgct ggaatccata agccaaaatc tgcccgtttt tatcggctgt ctctgccatc
    38581 tttcttgctt gatcagctgt catagctggt ggtttctcgc acatcactga agcattagct
    38641 tcgagggcag ccaatacatg ttcgaagtga aagcgattgg gtgtacagac actcacgata
    38701 tcgggagaaa tctcattcag catttgtgta actgatgtat acgcatgaga aaatcctgta
    38761 cgtcggcaaa agtcttctgc tcgctggctg actggatcaa ccacagcggc caattctaag
    38821 acatctgatc ttttttgata ataagcagca tgtacttttt ctgctacttg tccagcgcct
    38881 atgattgcta ctttcttcat ttatttctcc ctcctttgct tatttactta ttgttattct
    38941 ttctctattc tttctctaaa tcatttaaat gacgcgctgt ttccagacag tttttcaagt
    39001 attgcaatga ttcctgataa attttcactt cattttctcc tcgcaaacga cattcatata
    39061 ccatataacc ttcatactca ttttgaagta gcgtttgaaa atggcgagta aaatcaattg
    39121 cgccagatcc tggttggtag cgatgattat ctgctaaatg gatatgaccc acatactctt
    39181 tttgcgtagt caatgctttt gaaatatcat cttcttcaat attcatatga taaaaatcag
    39241 cgatgatctg cacatgtttc aattgattag agtcaatgta atgacgcgca tccgctaaca
    39301 aattgatcat atggtcttgg tatcggttca aaggctccaa ataaataacc gtacctgttt
    39361 cttcggctac tttatctaaa taacgaagtg attcgctgac tgctttatag tctccttctt
    39421 tgcttcgggg tgaaaccatc ggcggtaatc gataagtgaa cattccccaa gctgctggga
    39481 taacgattcc tttgcctcca acctcgctta gtgcacgcaa aatctcagca atttgacgta
    39541 caccattaag acgccgttct tcgataaagt caccgatcca cccatcgtag ccgttacaag
    39601 ctgtcactac tgggatccct gtttctttga ttgcctgttt gactgtgtcg atattttcta
    39661 ttaacaactt accgtcgatt tcgtattctt caaatccaca gtctttgata aatctaaatt
    39721 tttctaaaat gtctcttggg aaaaatgctt gatcttgcgt accgaatttc attgttctcc
    39781 tcctcggact tgaacgccca ttttgatact ttcttctggg tgctggtcta cataacgcat
    39841 gtaagcttct gcgctttctg caaatggcac aactggatca atgatttctt cacaatccaa
    39901 gtaaccgttc atcaataatt cccaacatgt gtcctcgatt cgtttacggt tccatcttgg
    39961 gtaatctggg tttggttcac tagatgctcg agcaaagacg atttttgcat tattgaaatg
    40021 tgcttcgcga ccaagattaa acccagctgg aaaaggttta gcaaaggcaa cataagaaat
    40081 gatcccgcca tagccgatcc ctcttaaaga agcttgtaat gcggcagcac tgccactcgt
    40141 ttcaataact gaatctactc cacgtttatc tgtcaattcc tttagacgaa aaccgacatc
    40201 tgctttcata ggatctagcg tgtaatcggc accgtatttt tctgctaatt ctctacgctg
    40261 cgctatggga tcgatcccaa cgatggtagt agcacctgct tttttagcta attgaatcgc
    40321 aatttgcccg atagcgccta aaccgaagat tgctacagca tcacctgctc gaacatgtgc
    40381 ttcccggata ccacctaaag cgaattgagc aggatcgtaa catactgcat ttttccattg
    40441 atccaaccga tcaagtttac gtagtttata attatcaaca ccgtttgtaa tcagcgtttc
    40501 acgaatgggt ccataagtgc agactaactc tcctttttga tactctgtca ccaaactgcc
    40561 cgtttcttcg atcgtaccga caatcatatt tcctaattga aattcgccaa ataccacacc
    40621 gcgttggctt ccttcttctc ttggcaagaa taggcgccat tcttctgaaa attcttcatc
    40681 gataaatgga cttaatcctc taaagtcgac aatttctgtt cctggttttg gagtagcgaa
    40741 aattactcgg atacgtactt cgttcttttc gatctcacgg tcttcgtaat ctttgaatcc
    40801 ggctattctt ggttctaatg caactaattt tttcatcttt tctttcctct ttcttttctt
    40861 tttatccttt tacgccgcct tcgactaaat cacctttaat aaatcgttca gataacgcat
    40921 acataatgat gactggtaaa gcggtcacca atgaagcggc catcattcgt ccccaaatgt
    40981 agtctggtgt actgaataat gtgttcaaac cgattggtaa agtaaatttt tcggaggtcg
    41041 acaggaaaat cgaagcaaat aaataatcat tccacgaaac cataaagcaa taaacgaaga
    41101 cagaaatgat tccagaaata gccaatggta taatgatccg aataatgatc tggatccggc
    41161 taagaccatc gatcattgct gcttcttcaa tatcagtcgg aatcgtgtca aaataacttt
    41221 ttagcatgaa tatcgcagta ggaagtgttt gaacgaccat cgtgataatc agtgctgtat
    41281 tggtatcata caaacctaga ccggaaatga ttttaaataa tggaacaacg atcaaaatac
    41341 cggaaaacat ataaacggtg taaaagctag cattgatttt cgagcgagct ttgaagcgta
    41401 gttttgacaa agcgtatgcc ccgctgatcc caatcactac tgcagttacg gaggctatca
    41461 aggagatcac taaactattt ttgaaataca tgatgaatgg aaaaatctca gcattgaaaa
    41521 tatcctgata atgttcaaaa gtccattctt tcggaaaaat agtttgtgtc gttgcaattg
    41581 cttcttgtga atccttgaat gatgtcatca gcatgatcaa aaatggaaac aaattgacag
    41641 ctaatagcaa caaaatcgtc gcgtaaaata agattttcct gctcttttta tttttcattt
    41701 caagtccacc ttctttcgtg agagcaagac gaccgctgca ataatgatga acaatagaat
    41761 cgatatagct gctgcttttc caagatcatt gaacgcaaag gctgtctgat acaaataaac
    41821 gcctaagata ttgactttat tcgtcagtag atagacatct gtaaacatat agaacatcca
    41881 gatagatcgt aaaacaatca cagttgctag tactggtttg attgccggca gtgtcacgat
    41941 cttgaacttc tgccaagcat ttgcaccgtc catttcagct gcttcataaa gcgttttgtc
    42001 gactgtttgc aaaatagcca aaaatgaaat aaacgcatac ggatagtatc tccagatagc
    42061 aaacaaaacg accataaaga agctagcaac tggctggtcg aaccacagcg gtgcttcgtt
    42121 gaaaagctgc aacctatcta ctaacagatc attgacgatt ccataactat tattgaacat
    42181 atatttccaa gcaaaaatca aagaaataga tggcgtaaca taacttaata aaatcagtga
    42241 acgcaccgtt ttcctaaatc tgaacttgcg gttaagcaaa atagctacgg ctaatcctaa
    42301 agctgtactg cctgctacaa ctaagaatgt gtaagcaact gttaaaccta aagaacgata
    42361 aaattcacta tcacttaaaa tttcaagata attgcttaat ccgataaaaa cggcgtctga
    42421 gttaggattc agcggcatat cgaaaaaact gatctgaata ttggaaagca ttggaatcag
    42481 cactaaaaca gctaataaaa gcatgcttgg tgctaaaagt cccatggcta attttaagtc
    42541 agaatgtttc cttggacgat tcattgaaac ctccccctga taatctgcca atagctatct
    42601 acttttttcg aacaccattg gcagaacttc ttgttatttc gtattttttt gtgcgtcagc
    42661 taaagcttga tcgacatctt cgtttccgac agttacttgg ttcactgctt ttccgatcac
    42721 acctgagctt gtgatatctc ccattttcgt gaaattcttt tccccgacta gtccaaagac
    42781 ttgtacttca tcaaaagcac tagcaatctc agatggcagc tcgccaaaag acttgatgac
    42841 ttcattttct ttgtaaccat ccagttcgac tacttgttta ttcactggtt gagcaccacc
    42901 aggagacatc aagacccact tttccatgtt tttcggttga gataagaaag ctgtgaatgc
    42961 ttcagcagcc tctttttgtt tctcgtctag accagaagaa atcgtcaatc cagatactgt
    43021 gccatagaca gcttgatctt tttgtgttgg gatagcaaaa ccgatatttt ctgagttccc
    43081 cgcttcataa acagaaggca aaatgtaagt tgaatagatt gccattggtg ctgttccatt
    43141 catgaaagca tctttgattt ctgtcacatc attagagcct ggcatcgtgt attgggataa
    43201 gttttgataa aacgacaatg cttctctcat cttttccgta tcgatcgtta cctcgccttc
    43261 atcatccaaa acatttgctc catttgacaa agcaaattga gagaacgctt gctcactcat
    43321 tgttccttca gcagtaggca tagcaattcc atattgttta ttctctttat ctgtgaaatg
    43381 ttgagcaatt tttaaaatat catcccaatt ttctggttct gaaaaaccag cttctgataa
    43441 tttttctttg tcataccaaa taccttgaac ccagccgcta attggtgcag caatatagct
    43501 ttttccatct tctgaacgaa caaggttttt ggctccgtca taataattat cttctccagc
    43561 ttcctcgata acgttttgga ctgccgtctg atcaatcagt tcatctttat ccatcacttt
    43621 agcaaaatcc tgactgactt ccattacttc tggtaattta ccggaacgag ctaacgtaac
    43681 gatttttgta ttgaacgctg attcttctac cggcacttgc ttgattttga tgtctggatt
    43741 ttctttttca aaatcagcaa ccagttcatt gatcacactt aatcgttcct gttcgacaga
    43801 agagtgcatg aattgaatcg ttgtctgttg tgattcgtta gagctttgac tttcaccaca
    43861 agcagtaaga agaaatccag tagctagcag acaggtagta acaataattt ttttcatttt
    43921 tttccacctc ttattttatt attttcagcc acatatattc atacggctgg aatatcaact
    43981 ttcgttcttg actgcaatgg ataatttgtc cagaaaacag gtctaggtaa tctccttttg
    44041 gtaaaagata acctgctgat tgattggaaa aattatggat aactaagact ttctcttcct
    44101 tcgcttcgcg aataaaactg ataaaagaat ctgctgagtc cagtaagtaa atcaaactat
    44161 tcggatggaa ggctttttct ttttttcgaa tagatagcat ctgggtcaat tcaccatata
    44221 ctgctgttct taatggatct gtctgcaact ctttctcaat atctgctaac aaaaattttt
    44281 gccgattgat ttttcgattc tctcctgttt tttctacgcc tttgtaatca tttcgacttc
    44341 ccagcacact ttgaatataa attgccggca cgcctggtaa cgttaccaaa aatgaatgag
    44401 cagctaaaaa gcgtttgctt ctccattccg tcgtatctgt tggagaactt aacgcatcta
    44461 gatacgtagc gtttatctca tatggactgg tcgttccatc tgggtttttc ttgtagttta
    44521 ctcgggtacc ctctttcttc agctgttcca ctaattcaag gatcgttttt tccggcagaa
    44581 tacctcgtac cggattcaat ccgataccgt catgggaggc taaaaaatta aaaaaagttg
    44641 tttttttcga tgcgaaatcg atggttttta accattcttt caacttactt gtattacccg
    44701 aacgaatcgc ttgaagggtc aaaggtggta gcgaaaactg atagaccatc tgtgcttcat
    44761 tctcgccatt cccaaaataa ctgatattat ctttgtgtgg cacatttgtc tcagtgatca
    44821 ataccgttcc tggagctgct tcatcagcga ttgcacggaa taattggatg attttatgcg
    44881 tttctggcag gtgtatacaa gaagttccta gttctttcca taaaaatccc acagcgtcca
    44941 agcgaataaa tgaagcgcct ttttctaagt aaaacaaaag aatatcgatc attttcaata
    45001 aaacttctgg atttttaaaa ttcaagtcta cttgatcttc gctaaaagtc gtccatacgt
    45061 gtttttcttt cccgtttttg tcaatgaacg ttgtaagtaa cgggctggtc cttggtctag
    45121 tcacgctgct tgtatctgta tccagtggca aatcaataaa aaaatctttg tacgtatcat
    45181 tcaagtttaa atattcctga aaccaagtac tttcggcaga catatgattg cagacaaagt
    45241 cgaacattaa ccgtgtttcg cttctcattt cttctatatc ttcccatgtg cccaaacggt
    45301 cgttgattgc ctgataattt acaacggaga aaccatcatc agatgtatat gggaaaaatg
    45361 gcaagaaatg tacaactcta aatgtatctg ccaaatactt ttgataaaat ttcttaaatg
    45421 tacgcaaagt tggttgctgc tcttcaaaaa attgatcccc ataagtaatc aagaaaatat
    45481 cttcttcgtc ccaatgcagt ttgcctgcct ggcctttctt ctgctgataa acactgactt
    45541 tttgttcaat catagaggcg gttttctcag acaaatctga gtcgcgataa atcgcttgta
    45601 aaagttttgt atacatcgga ttcccctttc ttttttggga gcgttcccaa cgatttgatc
    45661 atattatgga aaacgcttac tgtcaatagc atttttttaa ataattttga tatacacaaa
    45721 aatacaaata agaaagctta tgtatcaata attaatagct tcttaaaaaa atatggtctc
    45781 tcatgatcat gcaagctatt cctaaatttt caaaatcata gacaaaaaag aatgtctatt
    45841 gtactatgga attattctaa aacgctccca aaaaggaggt gcatataaaa ttgaaaccta
    45901 ctatttatga cattgcgaaa aaagctaatg tctcaaaatc aactgtctcc agagtattaa
    45961 ataaccagcc aaatatctct gataaagcaa aagcaagtgt acttcaagca atcaaagaat
    46021 tgaattatga accgagcaaa attgcaagag gactgtcagg aaatggtttt gacgcgattc
    46081 tagcattatc tcatcgtact tctcatacga cctatggcaa tccttttttt tcagatgtta
    46141 ttcaatccat ttctgccgtt tctgaacaaa ataacttcga cttgattcta caaacggcaa
    46201 aaacaccaga agaagagcta gaaaaatgcg cgaaaaagat ccaagaaaaa atgatccgag
    46261 gaattattat tttaagctct acgatggatg aaacacttct ttttgaattg gatcaataca
    46321 atattcctat tgtggtaatc ggaaaagtcg gaaacgacta cacgaacatc tactcagtgg
    46381 acacggataa tttccaggat agttacgatt tggtggaaac actgatcaag aatggacacc
    46441 gtcatatcgg ctgtctccat gctccgacag aagtttatgt atccaatgat cgtctcaatg
    46501 gttaccgtca atgcttattt gatcaccacc tagatatccg taatgaatgg attatagacg
    46561 gcggctttcg cttagaagat agcatggcag caatgaatga tttaatgaag ctacgagaaa
    46621 gaccaactgc gattttttct accgatgcat taaaatcatt gagtatttac aaagtggcag
    46681 aacaatctgg aaagcagata cctgatgatc tttcgatcgc aggattcagt gatcccttat
    46741 tgtcctccct attttcgcca cagttgactc ggatagacat tccgacaaat cagttaggaa
    46801 tcaaagccac agaactttta ttccaactaa tccaccacca ataccctgaa gaaaaaagaa
    46861 tcattctttc aaccgagatt actctcacag attcgatcca acaacaagaa atgtagaaca
    46921 gaattataaa agaacagaaa cactgaggat aagtcagtta ctctgttctt ttttcaatca
    46981 ctcatccctc gttaccccct cccggtattt tagcgaattt ttcttatttt ccatctgttt
    47041 tagatagctg tggataacta taatgtttgt gttaaactat tttttccaca gggaagaaaa
    47101 aactattttt aaccacgttg tgtataagtt tttatagttc gtttttttaa acacatttac
    47161 ccttatttca atccatgtta tttggggata cttatctcta aaacataaat agaataacag
    47221 acatgtctac tggttttgtt ttattcgagg aattattcaa taatagctag caaaatatta
    47281 aaaataaccg ctctatcttc ccatcaattt attttgcatt caaaaaatga tctatcttta
    47341 cttttgttga ttgacttctc ttggaagatg atcgttccga taccttaatt ggatacgaac
    47401 cgatgttgtt aagatccttt cccacttttc ttctagttag acaaattatt ttagac
//
LOCUS       DACCYH010000010        46140 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.010, whole genome shotgun sequence.
ACCESSION   DACCYH010000010 DACCYH010000000
VERSION     DACCYH010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.010"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            14..646
                     /locus_tag="IVY85_000550"
     CDS             14..646
                     /locus_tag="IVY85_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043587.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(780..905)
                     /locus_tag="IVY85_000551"
     CDS             complement(780..905)
                     /locus_tag="IVY85_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8043588.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1039..1932)
                     /locus_tag="IVY85_000552"
     CDS             complement(1039..1932)
                     /locus_tag="IVY85_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP8043589.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2114..3040)
                     /locus_tag="IVY85_000553"
     CDS             complement(2114..3040)
                     /locus_tag="IVY85_000553"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP8043590.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3125..3886)
                     /gene="pflA"
                     /locus_tag="IVY85_000554"
     CDS             complement(3125..3886)
                     /gene="pflA"
                     /locus_tag="IVY85_000554"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP8043591.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4130..6361)
                     /gene="pflB"
                     /locus_tag="IVY85_000555"
     CDS             complement(4130..6361)
                     /gene="pflB"
                     /locus_tag="IVY85_000555"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP8043592.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6638..9088)
                     /gene="parC"
                     /locus_tag="IVY85_000556"
     CDS             complement(6638..9088)
                     /gene="parC"
                     /locus_tag="IVY85_000556"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP8043593.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9100..11145)
                     /gene="parE"
                     /locus_tag="IVY85_000557"
     CDS             complement(9100..11145)
                     /gene="parE"
                     /locus_tag="IVY85_000557"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP8043594.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11311..11745)
                     /locus_tag="IVY85_000558"
     CDS             complement(11311..11745)
                     /locus_tag="IVY85_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP8043595.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11872..12510
                     /gene="plsY"
                     /locus_tag="IVY85_000559"
     CDS             11872..12510
                     /gene="plsY"
                     /locus_tag="IVY85_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP8043596.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12642..13517)
                     /locus_tag="IVY85_000560"
     CDS             complement(12642..13517)
                     /locus_tag="IVY85_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP8043597.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13599..14390)
                     /gene="codY"
                     /locus_tag="IVY85_000561"
     CDS             complement(13599..14390)
                     /gene="codY"
                     /locus_tag="IVY85_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP8043598.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14408..15808)
                     /gene="hslU"
                     /locus_tag="IVY85_000562"
     CDS             complement(14408..15808)
                     /gene="hslU"
                     /locus_tag="IVY85_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP8043599.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15822..16370)
                     /gene="hslV"
                     /locus_tag="IVY85_000563"
     CDS             complement(15822..16370)
                     /gene="hslV"
                     /locus_tag="IVY85_000563"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP8043600.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16407..17312)
                     /gene="xerC"
                     /locus_tag="IVY85_000564"
     CDS             complement(16407..17312)
                     /gene="xerC"
                     /locus_tag="IVY85_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP8043601.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17510..18886)
                     /gene="trmFO"
                     /locus_tag="IVY85_000565"
     CDS             complement(17510..18886)
                     /gene="trmFO"
                     /locus_tag="IVY85_000565"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP8043602.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18855..20933)
                     /gene="topA"
                     /locus_tag="IVY85_000566"
     CDS             complement(18855..20933)
                     /gene="topA"
                     /locus_tag="IVY85_000566"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP8043603.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21055..21912)
                     /gene="dprA"
                     /locus_tag="IVY85_000567"
     CDS             complement(21055..21912)
                     /gene="dprA"
                     /locus_tag="IVY85_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP8043604.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21968..22735)
                     /locus_tag="IVY85_000568"
     CDS             complement(21968..22735)
                     /locus_tag="IVY85_000568"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP8043605.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22728..23588)
                     /gene="ylqF"
                     /locus_tag="IVY85_000569"
     CDS             complement(22728..23588)
                     /gene="ylqF"
                     /locus_tag="IVY85_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP8043606.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23848..24246
                     /locus_tag="IVY85_000570"
     CDS             23848..24246
                     /locus_tag="IVY85_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8043607.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24483..25025
                     /locus_tag="IVY85_000571"
     CDS             24483..25025
                     /locus_tag="IVY85_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8043608.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(25108..25659)
                     /gene="lepB"
                     /locus_tag="IVY85_000572"
     CDS             complement(25108..25659)
                     /gene="lepB"
                     /locus_tag="IVY85_000572"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8043609.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(25811..26038)
                     /locus_tag="IVY85_000573"
     CDS             complement(25811..26038)
                     /locus_tag="IVY85_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP8043610.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(26035..26553)
                     /gene="msrA"
                     /locus_tag="IVY85_000574"
     CDS             complement(26035..26553)
                     /gene="msrA"
                     /locus_tag="IVY85_000574"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8043611.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(26573..27286)
                     /locus_tag="IVY85_000575"
     CDS             complement(26573..27286)
                     /locus_tag="IVY85_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP8043612.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(27289..28185)
                     /locus_tag="IVY85_000576"
     CDS             complement(27289..28185)
                     /locus_tag="IVY85_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8043613.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(28343..29185)
                     /locus_tag="IVY85_000577"
     CDS             complement(28343..29185)
                     /locus_tag="IVY85_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8043614.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            29317..29970
                     /locus_tag="IVY85_000578"
     CDS             29317..29970
                     /locus_tag="IVY85_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP8043615.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(30033..30560)
                     /locus_tag="IVY85_000579"
     CDS             complement(30033..30560)
                     /locus_tag="IVY85_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8043616.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(30580..31527)
                     /locus_tag="IVY85_000580"
     CDS             complement(30580..31527)
                     /locus_tag="IVY85_000580"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8043617.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(31539..33425)
                     /locus_tag="IVY85_000581"
     CDS             complement(31539..33425)
                     /locus_tag="IVY85_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8043618.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(33585..35663)
                     /locus_tag="IVY85_000582"
     CDS             complement(33585..35663)
                     /locus_tag="IVY85_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8043619.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(35676..35951)
                     /locus_tag="IVY85_000583"
     CDS             complement(35676..35951)
                     /locus_tag="IVY85_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043620.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(36313..36768)
                     /locus_tag="IVY85_000584"
     CDS             complement(36313..36768)
                     /locus_tag="IVY85_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043621.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(36922..38130)
                     /locus_tag="IVY85_000585"
     CDS             complement(36922..38130)
                     /locus_tag="IVY85_000585"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP8043622.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(38242..38574)
                     /locus_tag="IVY85_000586"
     CDS             complement(38242..38574)
                     /locus_tag="IVY85_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8043623.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            38722..39597
                     /locus_tag="IVY85_000587"
     CDS             38722..39597
                     /locus_tag="IVY85_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8043624.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(39679..40260)
                     /locus_tag="IVY85_000588"
     CDS             complement(39679..40260)
                     /locus_tag="IVY85_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP8043625.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(40266..41525)
                     /locus_tag="IVY85_000589"
     CDS             complement(40266..41525)
                     /locus_tag="IVY85_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8043626.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(41527..42573)
                     /locus_tag="IVY85_000590"
     CDS             complement(41527..42573)
                     /locus_tag="IVY85_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043627.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(42808..43083)
                     /locus_tag="IVY85_000591"
     CDS             complement(42808..43083)
                     /locus_tag="IVY85_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP8043628.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(43326..44636)
                     /locus_tag="IVY85_000592"
     CDS             complement(43326..44636)
                     /locus_tag="IVY85_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP8043629.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(44818..46047)
                     /gene="rpsA"
                     /locus_tag="IVY85_000593"
     CDS             complement(44818..46047)
                     /gene="rpsA"
                     /locus_tag="IVY85_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP8043630.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
BASE COUNT        12455 a         9728 c         7874 g        16083 t
ORIGIN      
        1 atttggagat attatggaaa ttcaacgtag aaccactaat tttttacatt taggctcttt
       61 ttttgcaact ttgacgatct tattttttct atgttattca taccttaaga cctcacatgc
      121 gccttctagt gcattaggct ggttgctttt ggatcattct gatatcacag aagccgaaga
      181 aaatccgttt tttttgattt tgtcttctct ttgcggctta ctgttttctg tttatttcgg
      241 tgcctatttt tttcatcgac atcctggtac gttaaacgaa gcttcaagcg tattgattgc
      301 atttaaaacg ttttcttttt tagcaattct tttttattcg tttaatcaca atacgctttt
      361 cttctttatc atgaatggtt tagtcgtgat tttatccatt tttacttatt ttattttttc
      421 actcattttt agccaattga aatgtccgct cttctttcga ttagttcctg tcacctgttt
      481 tatttacagc ttgttttcct tttttgtcct tgctttaatg ccactgacca acccttcgac
      541 gatccaagtc gcaggaaatc tgttggatat gctcttcatc gtcagtctag gtgtatttat
      601 gtggcaaaga aaaaacaaac aaaataaaaa gcattttgaa gcctgactat tacgacttca
      661 taaatgcttt tttagtttta aacacttcaa tctaaaagaa taacccaaga ttggataaat
      721 tattaatgac tcgccattcg tttcttagtg attctggatt atcaaatgga cttcctaatc
      781 taatcagcta gttcttccca taaaatgcca tacttgactg aatcataata ttgattattt
      841 aaaaatctaa cttttcttat cactacttcc tttgtcattc cacatttctc accatttttt
      901 tgcattcctt tgtttccaga ccatgtagtg aatctaatag gtggtagata ggcaaaatac
      961 tcaaataatt cttttaacca taaaggaaga gaaaggctcc tcctgcaaga gtcagtataa
     1021 tttttgctta ctgctctctt aggttttact cactaaaact gttccatatt ctttgatata
     1081 agaaaatgca tgcggtataa atttcttgct cagctcagaa aagatttgtc gtttaagaaa
     1141 ggccatttcc tccattgtca atgaaggatc caatgttggt aattcaatct caaaattgtt
     1201 tttttcgaca ataggtaatt ctaatgtgaa tgattcttct tcctttatag tattcgtcac
     1261 aagtagagac tgatggaaaa tactaggccg gcgattttta tatttattct tacgtatcct
     1321 gtcaacgata acttctattt gatcaaaaaa gtacgattca tttacgtctc caataagttc
     1381 ggctaattct ttgaatgcct tcgttacagg aagctgattc ttctgtacaa aaaagtaatt
     1441 agccaaagta agcgggaagt gttctataga ggaaagtgtg ataaaacggt acttctcatt
     1501 tcctgcagtt gtcctcagca attctgtctc tcggctgttt cttactccat aaaaatacgc
     1561 ttctttccgt tcatcttcga aacaatacgc ccaaataact tctcccatcg ctaatttttg
     1621 ttcatcaaca gagagtctct tcaattgttc agctatggtt tcagcagccg aagtggcttg
     1681 ttgcaagtaa tctttaggat caaatattgg aaagttcaaa tgatatcttc gttcttccct
     1741 cttgattaat cctgcatcga tcaataaatc aagaaaatgg gaaaatttat tttgcgaaaa
     1801 ttcttttttc aattctctta gaatcgtttc ttgttctcga tgttcatata gataatcggt
     1861 caatgaagaa aaaagcggat catcggtcag tttttgataa aagttttcct tattttttga
     1921 ataatagttc atatatgttc tcttttctat ccgttttatt ttacttaaaa ggcttttacg
     1981 taaaaaggag gaagaacaag agtcataatt gactgttatt ctcctccatt tcttcggcct
     2041 ttagtatgtt ctatgtttct ttttatatca atcgcttatc cattcatttt gaatgcttca
     2101 tactaccatt tgattagcta aatacagccg tcaattgagg tacgacttgt tttttacgtg
     2161 aaacgacacc tttaagcaaa gcacggttgt tggtcaaagt tgtttggaaa gcttcttcta
     2221 ctttttcttt tcctttacca gcaacaagta attctgaatc actgtctaag atgtttgtga
     2281 caactaatac gaacagatcg tagccatttg ctttcatttc atcttccatt gctgcttcta
     2341 gctctgcttg tcgatccatc acttcattaa ggtccactgt attgacttga gcgatacgaa
     2401 cgttggaact gcccattggg aaactttttg catccaaatc aagaagaacc gcagctgatt
     2461 tatcacttag atttgttcct gctttcaaca tttcaagtcc ataagtgttt agatcgactt
     2521 cagcgattgc tgcaagttct tttgcagctg cttcatcttc aggcgtacat gttggtgatt
     2581 tgaacaacaa tgtatctgaa atgatagctg agagcatgat tcctgcgatt tctttaggaa
     2641 ttgttacttg gttttcttta tatagtttta aaataatcgt acttgtacat ccaactggtt
     2701 ctgcacgata gtacaatgga ttagctgttt caaagtttgc aatacgatgg tggtctacta
     2761 cagctaaaat atttacttcg ctgatatctg aaatactttg ttggaattca ttatgatcca
     2821 caagcatgac atctgcattt tctgctttag ctgactctac tactcgtggt gcagatagct
     2881 gaaagtggtc taatgcgtat tgtgtttctt cattaggtgt acctagagca actgcttctg
     2941 tttctttacc taattcattt tgcaagtgtg aaaacgcaat ggctgccccg attgcatctg
     3001 tatctggatt ttggtgacca aaaactaaaa tttttgacat gtcattcgct cctttgtgat
     3061 ttctcctatt taataatagc aaaaaaacgg aaagaagcaa tacttatctt ctttccgatg
     3121 cgacctatcg cgttaaatat tttgtatact cttctgtatg aagcaatcgt ttagcatttt
     3181 cgacacgttc ttttgtaggt ggttcgatcc cttctaatgg atatttcaaa ccaagtgttt
     3241 cccacttgta ttttcccatc gtatgataag gaagaatctc tactctgtct acatttgaca
     3301 aggtttgaat aaagttgtct agtcggatca aatattcatc ataatcactt cgctcaggaa
     3361 ctagtacatg gcgaatccaa accggtttgt tgatttttga caaatattga gccatttcta
     3421 aaatattttc attgtcccaa tgcgttagtt ttttatgctc ttcattatcg atgtgtttga
     3481 tgtcaaataa cagtaaatct gtatacttca tcaattcttg aaaacgtgaa aagaaaggtt
     3541 cttcatacgt aaaaggtttt ccgcatgtat ccaatgtagt atgcatcttc cgctcttttg
     3601 cttttttaaa tagttcgatc aaaaaatcga tctgcaatag aggttctcca ccactaacag
     3661 taataccacc tttatctccc cagtaaggtc tgaagcgttc tgctttatct aaaagttcat
     3721 ctgctgtgta ttctttccct ccgccaatat tccaagtatc tggattgtga caaaattggc
     3781 aacgcatgcg gcatccttgc ataaacacga caaagcgcag tccaggtccg tctactgaac
     3841 cgaaagtttc gatagaatga acatagccga ttgttttttc ttccatttaa gttcacatct
     3901 tttctttgat ttcattttaa gtacaagtta gctgtcgaag cgaagctttc tgaacaaaat
     3961 ttttcgcaat tgccgcttcg ttatgtgtaa aatttacagg gacggaacat acaagtcctg
     4021 cccctgtaaa tcgtttcttt caaataagca tactgattat tcgattttga tgcgatggct
     4081 attcaacgtc ctttcgacgc taagtcgaaa aacttttttc acgaactttt tacattctgt
     4141 cgtgtgaagt tctcatcaag acatctaatt gttgttctct tgtcaagtca cggaatttaa
     4201 ctgcataacc agatacccgg atcgtcaagt tcggatattt cattggattt tccattgcat
     4261 cacgcaataa atcattactg aatacattga cattcaaatg ataagctcct tttgagaagt
     4321 atccatctaa gacattacgc aagttttcga ttcttgtatc ttgttctttt ccaagacctg
     4381 aaggattgat tgtttgggta tttgaaattc catccaatgc acagccgtaa ttcattttag
     4441 ctgttgagtt caggctagct aataacccac tttgttccgc attgtaactt ggatttgctc
     4501 ctggcgcaag tggttttcca gcttcacggc catcaggagt atttcctgta tttttaccgt
     4561 aaacaacatt tgaagtaatc gtcaataatg aagttgttgg ttttgagttg cggtaagttt
     4621 tatgacgttt gatttgtccc atgaagtatt ccaagatcca tgaaactaat tcatctgcac
     4681 ggtcatcatc atttccgtat ttagggaagt cgccatccac ttcaaaatca actgctattc
     4741 ctttttcatt acggattgct tttacttttg catatttgat cgctgacaag ctgtctgccg
     4801 catgagagat cccagcaata cctgtagcaa atgtgcgttg caagtcagaa tccatcaaag
     4861 ccagctgagc tgcttcataa gaatatttat catgcatata atgaatgatg ttcaacgtat
     4921 tgacatatag ttctgctaac caatccatca tattcatgaa gttatgtttc acttcttcaa
     4981 agtctagtat ttctgatgtg attggacgat attctggtcc aacttgggcg ccagtcactt
     5041 catcgactcc gccattgatg gcatataata gtgttttagc caagttagca cgagcgccaa
     5101 agaattgcat gtcttttccg acaactgttc ctgatacaca gcaagcaatc gcacaatcat
     5161 cagaacccca ttgttcacga agctgattat cattttcgaa ttgaatcgaa gaactttcga
     5221 tagcgattct gctagcatat gttctgaatc cttctggaag gtgttcagaa tacaaaacag
     5281 tcaagtttgg ttctggtgaa ggtcccatat ttgttaatgt atgaagcaga cggaaatcat
     5341 tctttgtaac taatgaacga ccatctaatc ccatacctgc aatggataaa gtagcccaga
     5401 tagggaatcc tgagaataat tggttatatt caggtgtacg cgcgaatttg accatacgta
     5461 atttcattac taagtggtcg atcaattctt gtgcttcttt ttctgtgatt actccattat
     5521 ccaaatcacg ttggatatag atatcaagga atgctgagat acgtccgatt gacatcgcag
     5581 caccattttg tgatttgatt gctgctaaat atccgaagta tagccattgg acagcttctt
     5641 ttgcatttgc agctggttta gaaatatcat agccatattt cgcagccatt tctttcattt
     5701 gcgctaatgc acgatattgt tcagtgattt cttcacgtaa acgaatcact tcttcactca
     5761 ttgtatgata accagtcata tcatgatctt ttttcttttg ttccatcaaa tagtcgattc
     5821 catacaatgc cacacgacga tagtcaccga tgatacgtcc gcgaccgtaa gcgtctggaa
     5881 gccctgtaat gattttgttt ttacgagcag cacgcatttc agcagtataa gcgtcaaata
     5941 caccttgatt atgtgttttg cgccagtcag tgaaaatatg tgtcaattca tcgtttggtg
     6001 tatagccatt gctttttagc gcattgttag ccatattgat tccgccgaac ggcatgaatg
     6061 cttgtttcaa aggtttgtct gtctgtagac cgacgatttt ttccaaatct ttatccaaat
     6121 agcctgcatc gtgtgaagta atagttgata cgatttctgt gtccatatca tagacaccgt
     6181 tacgctcgaa ttggatatca tttaactttt gcaattcatc ccataatttt tctgttgctt
     6241 ctgttgggcc ttccaaaaag ctgtcatcac cgcgatattc agtgtagtta ttttgaataa
     6301 agtctctagt gtctacgcct tctttccatt tggtaccttt aaatccattc cattgctcca
     6361 ttgtatggac ctcctaaatt tctgttttat gtaacagttt ttacgtgatt agtataacac
     6421 agcaccctat ctttgcaagg gttttcttgt tttttattta acaattttta tgttttattt
     6481 acatatattt aaatcaacgt tgacatacca acatttaagc attgtgaaca ctttcatctt
     6541 tttttgcaaa cgtataagga tttattaaaa atgaatgaaa aagagacgcc acctgtatta
     6601 atacagatgg cgtctcttca ttaaaatact acaacagtta cgactcgatg aaaatctcag
     6661 gagcatcgat aacttcgatg acttctccac cttttttctc atccatcgca aatgatccat
     6721 tcgatgttcg atcacctata gggaaatcag ttgggtcaat cgttaactgc tgcccttttt
     6781 gattcatcaa ggttatttct tttgatcttc cttccgacat atagataata cggtgcggat
     6841 tttttttcag ctcacgcatg accgtcactc cccgcttcgc acgtgaagtt tggctgattt
     6901 cttgagcaag catgcgttta acagctcctc gctgagtaac gattacgatc ggcgtatcac
     6961 cttctgctaa cacaagcagc ccgttgataa tcctatctcc atctttcaaa ttcatagact
     7021 tcactccagc tgcctttgct ccaacaacgg gaacatctgt taaaggatag cgcaaaccca
     7081 ttccttgatg cgttactaag aaaacatcta agcctggctg atcttctttc agatacacag
     7141 caatcagttc atcttcttcc gcctttagct tcatacagct gagcggacgg ctcttatagg
     7201 tgcgccatgg ttcgaactct gtcattcgtg tttgcttgat caatccattc ttgctgatga
     7261 atacaaatgt tttctctgct tgaatttttt gatatgggaa aacagctaaa atcgattcgt
     7321 ctacagacag attggagatt gtttgagaaa tgtgctcacc agcatctttc catttcaaat
     7381 cgggtagttc atggacaggg cggtagatca catttgcttt atttgtgatc agaagaacat
     7441 ggtccaatgt actcaattca cctgagtata gcaagtagtc gccttctcgc atcccgattt
     7501 cttctggttt ggaagcacta taagaacgaa ggcttgttcg cttcacatag ccttctcgcg
     7561 tcacactgac aattacgtct tcttgtgcca ctaagacttg cgtatcaatt ttgatttctt
     7621 cgatttcgtc ttcgattgtc gttagacgag cggaagaata attttttttc acttctcgca
     7681 attctttttt cataacagag aaaagctctt tgtcatttga aaggatttta ttcaactctg
     7741 tgatttgcgc aatcaagctt tcggattcct tttgaagctc tgtgatatcc gtgttcgtca
     7801 aacggtataa ttgcaatgtg acaattgctt ctgcttgttc ttctgtaaat tgaaagacgt
     7861 cgactaaatt ctttttggcg tcttttttgt ctttactttc acgaatcgtt gcgattactt
     7921 catccagaat cgataaagct ttcatcaatc cttctacgat atgctgtctt ttttgagctt
     7981 tggctaaatc gaattgacta cgttttgtta tgacttgctt tcgatgctcg atataacttc
     8041 ttaaaatgtc tttcagtcca acttgatgcg gagtcatatg gtcgatagcg accatattga
     8101 aattgtaatt gatctgtagc tcagtgtttt tgaacaaata attcaaaatt ccttgagcat
     8161 tggcgtcttt tttcaattcg acgacaattt gcagacctgt tctatcactt tcatctcgta
     8221 cttcagctat tccatcgatt ttcttattca aacggatctc atccattttt ttcaccaagg
     8281 tcgctttatt tacttcatat gggatttctg tgatcacgat ttgctgttta ttacccttca
     8341 ttgcctctat ctttgttttc gaacgaagaa taacttttcc tcgtcctgtt tcataagctt
     8401 ttttgatttc atcttttcct tgcaagatcc ctcctgttgg aaagtcaggt ccaggaatat
     8461 attccatcaa tttttcaagg ctcgcttgtg gatgatcgat catatagatt gtgccatcga
     8521 tcacttcagc taagttatga gtcgggattt ctgttgcata tccagcagat atacctgtcg
     8581 atccattgac gagaaggttt gggtatcgag ctggaaggac tgttggttct ttttctgtat
     8641 catcaaaatt ccacacgaga tcgactgttt ctttctcgat atctgctagc atctctccac
     8701 ttaacttgga taatctggct tcagtataac gcatcgcagc aggtggatca ccgtccatac
     8761 tcccgttatt cccgtgcatt tcgatcagta cttcccgcaa cttccagtct tggctcatgc
     8821 ggaccatcgc ttcataaata ctgatgtcac catgaggatg ataattcccc atgatatttc
     8881 cgacagattt tgctgattta cggaatcctt tgtcatacgt attgccatct ttgttcatgg
     8941 caaataatat ccggcgttgc actggcttca atccatcacg gatatcaggc agtgcacgtt
     9001 cttggataat atatttggaa taacgtccga agcggtcacc catcacttct tctaaagtta
     9061 attcttgtac ttcttgtcga ttttccatca ggtgacctcc tattctactt caaataactg
     9121 attgttttca tttttatctg ctcgttcttc atctaataaa tcattagaaa ctgaaggaga
     9181 gatttcagta tcctcttttt tatctaagat actgccatct tcttcgagcg taaattgcac
     9241 gtgattttcg atccattttc ttcgaggttc tactttgtca cccatcagtg tggttactcg
     9301 acgttcagct tgcgcagcat catcgatacg gacgcggatc aacgtacggg aagtcggatc
     9361 catcgttgtt tcccacaatt gttccgcatt catctctcca agtcctttat atcgctgcag
     9421 catatatcct ttgccgactt ttttaatcat tgctgccaat tcgtcatctg tccacgcata
     9481 ttcaatgact tgcttctttc cttgtccttt ggatacttta tataaaggcg gtaacgcgat
     9541 atatactttc ccagcttcaa tcagcggttt catataacga taaaagaaag taagcagtaa
     9601 tacttgaata tgggcgccgt ctgtatccgc atcggtcatg atgattactt tgtcgtaatt
     9661 acagtcttcg atagaaaact caggaccgac tcctgcacca atcgtataga tcatcgtatt
     9721 gatttcttca tttttcaaga tatcttgcat tttagctttt tcggtattga tgacttttcc
     9781 tcgtaaaggt agaattgctt ggaactttct gtctcttcct tgtttagcag atccgccagc
     9841 agaatcccct tcgactaaat acaattcatt tttcttaggg tttcgggatt gtgcgggtgt
     9901 caattttcct gaaagaagag attcgccttt tttacgcttc ttcccatttc ggctttcttc
     9961 tctggcttta cgtgctgctt ctctcgcttc tctggctttg attgctttac ggaccaacat
    10021 ctggctcatt tcgctatttt cctgcaaata aaagcccatt tgttcactga tgacgttatc
    10081 tactacggta cgagcaactg gcgtaccaag tttttctttt gtttgccctt caaattgcag
    10141 aagattttca ggtacacgga tagaaagtac agcagctaac ccttctctga aatcactacc
    10201 ttccagattc ttgtcccgtt ctttcagcaa accaactttt ctcgcatatt cattataaga
    10261 tttcgtcatc gccgctttca taccagcttc atgggttccc ccatcttttg tccgaacgtt
    10321 attgacaaaa gataaaacat tttctgaata accgtcattg tattgataag cgacctctac
    10381 ttcgatacct tctttttctc ctgaaaaata aacaactggt gtcaatgtat ctttttcttc
    10441 attcagataa tcaacaaatt ctttgattcc ttcttcgtaa tgaaaaattt ccttgactgg
    10501 ttcttctcca cgaagatcag aaagagagat tttaacgcct tttagaagaa aggcagattc
    10561 tcttaaccgt tcggctaaaa tttcatatga aaatttcgtt gtactgaata ttgtatcatc
    10621 tggtaaaaaa tggacagtcg tcccgtttgc tttttttgtt tttcctatct ttttcaaagt
    10681 accgtcaggt ttaccgccgt ttttaaattt ctgctgatac tctacgccat ctctaacgat
    10741 cgttacaatt agccatttcg ataaggcatt taccacactg gctcctacac cgtgcagtcc
    10801 accagaagtt ttgtaccctc cttgaccaaa ctttccgcca gcatgcagca cagtaaaaat
    10861 cacttcaact gttggtattc cggaagcatg cattcctaca ggcatcccac gtccgctatc
    10921 tgcaacagtt atactattgt cttcatggat ggtcacatca atctcactgc cataacctga
    10981 taatgcttcg tcaactgcat tatcgacgat ttcatagact aaatgatgca agcctcggct
    11041 gtcagtggat ccaatgtaca tccctggtct ttttctaact gcctctaatc cttccaatac
    11101 ttggatggac gagtcattgt attcgttgtt tacttttttt gccaaggcaa acgctccttc
    11161 aataatactt tgcggcaaaa ccgcaccacg attatcatac tagaaaaggt cgtgaaaaaa
    11221 aagagggtag acgagaaatc aattttaatt atctcctaac cgctttttcg acattcccct
    11281 ctatcgatca tctcttcttt gtggtcattt ttatttttgg ttttctttca acttaccaag
    11341 ttcaatctta atacaacgat ccattacgat atctgtacgt cctgcttttc ttagcatctc
    11401 agctgcttct ttgttttcaa tccctaattg tgcccagaac actttagcat cagttttcaa
    11461 aaaatcacct gctatttcag gtaaaaattc actccgtcta aatacatcta cgatatcgat
    11521 tggatcatcg atttctgtca aattcgcata gactttctcg cctagaattt cttcaccagc
    11581 caataatgga tttactggta tgattttata tccattgtct tgcaatacct gtgcaaccat
    11641 gtaacttgtt cgttctggtt tattgctcaa gcctacgacg gcaatatttt ttgcttcttc
    11701 caaatagttg aaaatcgttt cttgtgttgg attttttaca gacatttctg ccaccccttg
    11761 cttttctttt tattccatct attatacttg tatctttcat tttttcaatt gttcctgtta
    11821 cctattcatg ctaaaataaa ggatagtttt caacttgaaa gagggtaaaa aatgaaattg
    11881 atcttattac ttattttagc atatctttta ggttctattc cttccggtgt ctggattggc
    11941 aaattgtttt tcaaaaaaga tatccgacaa cacgggagcg gtaatactgg aacgaccaat
    12001 acatttcgtg tattaggcaa aacagccggg attgttgtcc tgcttatgga tattttaaaa
    12061 ggtacattgg ctacttgttt gccgatgatc ttccaccttt ctgggattaa ccctctttgg
    12121 tttggggtat gcgccattct tggccataca tttccgattt ttgctgggtt caaaggagga
    12181 aaagctgttg cgacaagtgc aggaatgctt ttaggattta atcctgtatt ttttgtttat
    12241 tcttgtatca tctttatcgt tagtttattt tgtacaagta tggtgagttt gagcagtatg
    12301 atcagtgcag tattgatcac tttatctacg atcgtccttc cttatgtagc tcctgtgatt
    12361 ttggctaagc ccaattggtt actgacgatc attgcctttt tagtttcttc ctttatcttt
    12421 tatcggcata aagacaatat caagcgtatc agaaaccata ctgaaagtcg aatcccgttt
    12481 ggtttgaata gcagcaagaa aaaatcctga tactttttca ttccaaagag ataaaacggg
    12541 agtgtgacaa atcgatgtca cactccctta ttacttacag gatagctttt ttacacacct
    12601 tggtaaacgg tgttccctgc taacttctcg gtcacaacct cttacttcac tgtgatggaa
    12661 tattttgtct tgaagatttc atgggcacct agttgattga tccctacttt ttccatcaag
    12721 tttccactac tgttcaccgt gtcagcgatt ccgcaccacg gttcgataca aacgaaaggc
    12781 gcttcttttg gataaggaga ccagatccct acataaggca tatctttata gcttaaagtt
    12841 acgctatggg aagatttttc acttcgaatg gcgtacgcat ttagacccct tgtttcaaaa
    12901 accaaggcat cttgctcaaa aagttcacga ttcaaagcca gattcgtatt tgtctgacct
    12961 aacgtctttt gatccatatc gatgaatggg cctttcaatg gtaggcgtaa tcttgatttc
    13021 aatggagaaa atgccagata gaaatcttca aatgacaatc cttcttcaag aggtacattg
    13081 aaagcaggat gtccaccgat agaaaagaac atttcttttt caccggtatt tgccacttga
    13141 taatgaaccg tgatgccgtc tcctcctaat tcatagctga tcactaattc aaaatcaaat
    13201 ggatactttt ctaatgtctc cggcgtactc cttaagatga aactagccat ttcttgtcca
    13261 tgttcaatga cttcaaaatc catatcccga gcaaatccgt gttgccccat ttgatatgtt
    13321 ttcccttgat aaacatactg atcatctttt aaacgtccaa caaaagggaa taaaaccggt
    13381 gcatgtctgc cccaatacgc tggatcacct tgccagatat actctaaacc atgttctttt
    13441 gatttcagac tgatcaattc cgcaccgtgt tcagcgattt cggcgatcag ctggtcattt
    13501 tgaataataa cagtcattgc cattcctcca acttcgactt actttttaaa atgggaaggc
    13561 tggaacaaac atttctatct gccccagtcc ccttacttct aataactgtg ttttgctaac
    13621 tcgtctttaa aacgggaatt caatactttt aaataagttc ccttcatgcc taacgaacga
    13681 gattcaatga ttccagctga ttccaatttt cgtaaagcat tgacgatcac agaacgagtg
    13741 atgccgatac tatcggcaat attagaagca gtcagacgac cttcatctcc atccaatgct
    13801 tcaaagatag cttgtacagc cttcaattcg ctataagata atgtgttgat tgccatttgg
    13861 acagccgttg cactccgtac atctgcttcg atgcttcgag acttctgata aaggatctgc
    13921 ataccgacaa ccgtagctcc atattctgct aagactagat cgtcatctgc aaacgattgt
    13981 tccatacgag ataagataat cgtacccaaa cgttcgccag ctccaaagat cggaacgaca
    14041 gtcgtcagac caaatggata tttctctctt gactctaccg gaaatgcagt aatatcacta
    14101 gtaattggga tattagcttc agttttcatc aactgtgcca ctgtatcagt ataagattca
    14161 ggaaattgtt tctcaacaaa cattgttttg attcgttcat tgttcacatc gtgacgttca
    14221 ttataaccta gtaatactcc gtcacttcct atgatatagg tgttgctgtc aagaatacca
    14281 cctagtgtga cagccatttt atcgtaaggc aaatctgctt gcatgtcaaa agtattcttt
    14341 tgctgcaaaa gtgtgtttat cttgcgggtt ttcgttagca agtctgtcat tgtcgtcctc
    14401 cgatttttta taaaatatag cgacttaaat cttcatttgc cgcaattgag ccaagtttgt
    14461 catccacata tgcttctgtg atcgttattt ctcccatttg catatctggt gcttcaaata
    14521 atagatcttc tagtagtttt tccaaaatgg tatgcagtct gcgtgcgccg atattatctg
    14581 tgtcacggtt gacatcgaaa gcgatttgag cgatccgttc aattgcttct tttgtaaacg
    14641 taactgacac attttctgta ccgatcaacg cgacgtattg ttttactaaa gcattatttg
    14701 gttcggtcag aatcttcaca aaatcttcag ctgtcaaatc atctaattct acccgtatcg
    14761 ggaagcgtcc ttgaagttct ggaattagat cacttggttt agacaaatgg aaagctcctg
    14821 aagcaatgaa taggacgtga tctgtttgga tgatcccgta ttttgtgttt acttgtgaac
    14881 cttcgacaat tggcaatatg tctctttgga cgccttctct ggatacttct ccagattgct
    14941 gtgatttaga agtgatctta tcgatttcat cgataaaaat aattccactt tcttccgcta
    15001 atttgattgc ctcactatgg atatcagcat ccttgactaa ttttgctgat tcttccttga
    15061 tcaataactc taaagcttct ttgactgtta ccgtccgttc cacttttttc ttcggtgtca
    15121 aagcacctag agtttcattt aggtcgatcc ccatttgctc caatccattg ttcatcattg
    15181 gtgcaggttt cttttggtct tcaacttcaa tggttacttc acgactatcc agtaaaccct
    15241 tttgtagttg ttccaagact gtacggcgat tgattcgaat atcttctgtc acttcttctt
    15301 gcggttcagc ggtattttgc tgactagcat taaaaagatt catcatttgt tcaaattgat
    15361 tagtggattg tttctgttct tttttgatac caggtaccaa gatttttacc aaacgttttt
    15421 ctgcattttt tttagcactt gtgtatacac ggctgtactg ctctttttct acgatttgga
    15481 tcgcattttc tactaaatcc cgaaccattg attctacatc tcttccgaca taaccgactt
    15541 cagtaaattt tgttgcttcg actttgacaa atggtgcatt gacgattttt gccaaacgtc
    15601 gtgcgatttc tgttttaccg acccctgtag gtccgatcat cagcatattt tttggtgtaa
    15661 tatcttgttg cattttttca tctaactgca aacggcgata gcggttacgc aaagcaacgg
    15721 caacagattt ttttgctgcg tgctgtccta cgatatattc atctaattca cttacgattt
    15781 gtcttggtgt ttgttttaat tcatacatat ctactcacct cttacagttc ttctacgata
    15841 atattatgat tagtaaagat acagatatct gcggcaatat ttaacgcatt ttctgcaatt
    15901 tctttcgcac tcatgtcgtt ttgagcatag tttttcattg ctcgggctgc agccaacgca
    15961 aaattccctc ctgatccgat agctaagata ccatcatctg gtgtgataac ttctcctgta
    16021 cctgaaacaa gaagcatttc tttatcattc attacgatca gcatcgcttc aagtttttgc
    16081 atagattgct gtgtccgcca ttcttgagct aattcgacag ctgcgcgttg tagattcccg
    16141 ttatattcgt ttagtttgcc ttcaaatttt tcttctaaag taaacgcatc tgcaacactt
    16201 ccggcgaaac ctacaacaac ttctccattg tagatacgtc gtactttctt tgcagtgcct
    16261 ttcatgacaa cagattcacc cattgttact tggccatctc cagccatagc aaatttcccg
    16321 tctttttcga cggcacagat agtggttgaa tgaaatggtg attcgaccat gtttgtttcc
    16381 tcctaaattt gttcttttcc ttatttttac gctcttggat ggaaagaacg atagtttttt
    16441 tgtaaacttt cttttgttac atgggcatag atctgggtag tcgataaatt ggcatgtcct
    16501 agcaattctt ggactgtccg caaatctgcc ccattattta ataaatgagt agcaaatgta
    16561 tggcgcagca tgtgaggatg gatatcacta tccaaactgc tttttttgat catttgattc
    16621 aagacatatt caattccagt aggcgtgatt ggttttccat gatgattcac aaaaacgata
    16681 tcatgctctt cgtgatattt tgtcatcaat acctggcggc cttccctgaa atattcttgc
    16741 agagcatctt gagcaaaaga acctagagga gcatatctct ctttgttccc tttaccatgg
    16801 atcaacatga cctctgaatc aaaatcaatg tctgataccg acaaactgct acactcactt
    16861 aaacggatgc ctgacccgta aaggacttcc agtaaagcgc ggttcctcaa atccaaagga
    16921 ctatctcctg ctacgctgtc aaaaagtgct tgcatttcat tttcataaaa aaaacgtggc
    16981 agtctcaaat ttttcttttt gagatgaacg taagaaaatg gattttcttt tatcacttct
    17041 tctttgagaa gatactgata aaacgaacgt aggctggcta tcttgcgact gatagaattt
    17101 ctgctatagc taggacgatg atcagctaat gcactcaaat agacacgaac cgtaaaatat
    17161 tccgccttca gcagatcagc gtctccagat tcctccaaaa aatgtttgaa atggagaatg
    17221 tcttcttcat atgcttcttt cgttttttct gaatatcctc tttcgacaat caaataacgc
    17281 aaaaattttt gttcccacgt ggcttctgtc attttctcac cttccttttg tcccagagaa
    17341 taatttagca cagcgtttat aaaaagacaa ataaattgtc agaatttaac caatatttct
    17401 aagtttgttc acaattggcg caattcctca cattttgttt gagaaattct taagaaaaac
    17461 gcaagaaaaa gagcgagact atccgtctca ccctcttcat ggagtttcct tactcatttg
    17521 ggattgcagt tttttctaat tctgttttta ttgcctcatt agcttttaaa gctcgttgtg
    17581 caattgcttc atatcttgct tttttgtctc gaatgcgctc tggcagttca ggaaacagac
    17641 catagttggc attcatcggt tggaaatgtt ttccttccgc atgagtaatg tagtaagcca
    17701 tacttccaat agctgtttct tgtggaaact cgactggttc catttctttt gctaagcgtg
    17761 cggcgttgat tccagcaagt agtccgctcg ctgcactttc aacatagcct tctacaccag
    17821 tcatctgacc tgcaaagaac agatcttcgc gtttcgttga ttggtaagtc ggtttcagca
    17881 attctggcga attcataaag ctgtttcggt gcatcacacc atagcgaacg aattcagcat
    17941 tttctaaccc aggaatcatc cggaaaacac gtttttgctc gccccatttc aagtgagtct
    18001 gaaagcccac gatgttgtat aaagaagctg cagcattgtc ttgacgcagc tggataacag
    18061 cataaggacg tttgccagtt ttaggatctt ccaatccaac tggtttcata ggtccgaaaa
    18121 gcatcgtttt gaatcctcgc tgtgccatta cttcaattgg catacatcct tcaaaatatt
    18181 tttctttctc aaaagattta agttctgcca cttcggcatt gattagtgcc tcatggaaag
    18241 ctttgaattc ctcttctgtc atcggacagt tcaagtaagc tgcttctcct ttgtcatagc
    18301 gggacttcaa atatacttta tccatatcga tcgtagcttt gtctacgatt ggtgcagctg
    18361 catcatagaa ataaaaacca tctgaaccat tgaatgcttt gatgctttct gctaatggat
    18421 gagaagtcaa aggtcctgtt gctactacgg tgattccttc cggtatttca gtaatttctt
    18481 cgtttatcac tgttactaat ggatgctctt tgacttttcg ggtaattgtt tcagaaaaag
    18541 aatcgcggtc aacagctaag gcaccaccag ctggcactgc tgtttcatct gccgaagtga
    18601 ttactaccga attcaatcgg cgcatttctt ctttcaataa ccctactgca ttagccaaac
    18661 tgtttcctct taatgaattg gaacatacta gttcagcaaa attacttgtg tgatgcgcct
    18721 cagtcgattt gactggacgc atttcatata aacggacagg gactcctgct tgtgcgatct
    18781 gccaagcagc ttcacttccg gcaagccctg ctccaacaac atttaccatt ttcaatgaaa
    18841 gaacctcttt tctattattt ttggatgtct tcttcgtaat ctccatttac acagactact
    18901 tgtttaccgc ctttgacttt cttctcgacc agatatccgt cacattttgg acaatttctg
    18961 cccactggtt tatcccaaga agtgaattca cattctggga aacgggaaca accatagaat
    19021 agtctgtttt tcttagattt tcgttcaatg acttgtcctt catgacatac tgggcaagtc
    19081 acaccgattt cttttacgat tggtttcgta ttccgacaat caggaaaatt actgcaagca
    19141 taaaacttcc catatttccc taattttatc accatcggat gtccacagac atcacaatcg
    19201 aatcctgctg gttcatcttt gatttgaatt ttttcgattt tttcttccgc attcgtcagt
    19261 tctttttcaa acggtttata gaaacgatca accactttga cccacgcttc cgtgccttct
    19321 tcaattttat ccaaatctcc ttccatttct gctgtgaaat ggatatcgac gatttgaggg
    19381 aagaattcac agattaatga attgacaatt tctcctagtt cagtcggctc aaatcttttt
    19441 tgtgtcaatt ttacatagta gcgtctttgg atcgtatcaa gtgttggtgc ataagtggaa
    19501 ggtcgcccta ccccgttttc ttctagtgca cgaatcaacg tagcctcact aaaacgtgct
    19561 ggtggttgag taaaatgttg ttttggttca atatcaacag attggactac atcgccttct
    19621 tcaagttctg gaagaatatt ttctttctct tctttgccgt catctctacc ttccacatac
    19681 acttgcatga atcctttgaa tttgattttt gaaccgttcg caatgaagat gacaccattt
    19741 tgttctaatg tcactttcat tgtatctaaa atagctggtg tcatctgact agccacaaaa
    19801 cgtgaccaga tcaatgtata aagtttcaat tgatctttat ctaaatattg tttgagttca
    19861 tctggtgtac gaagcacact cgttggacgc acagcttcat gggcatcttg tgcgccttga
    19921 gcatttttga tctttcggtg atcatgtcta gaaaattctt ttccatacgt atcttcaata
    19981 aatgctgcgg cctctgcttt tgctgtatcg gcaatccggg tagaatcagt acgcatgtat
    20041 gtaatcaagc cgactgttcc ttgtttaccc aaagtgattc cttcatataa ttgctgtgct
    20101 accatcatgg tttttcttgt tcgaaagttt aacttacggg cagcttcttg ttgcaggcta
    20161 cttgtagtga aaggattcgc agggtttctt ttgcgctctt tcttttctac ttttttaact
    20221 tcgaagtctg ttccatcaat tctagcagtg acttctttta cactttgtgc atctggtaat
    20281 ttctttttct taccgtcgat tccccagaaa tttgctttga atttcttgcg agctttttta
    20341 aaattaccgt cgatgctcca atattcttct ggaacaaatt ctcggatctc tttttcacgg
    20401 tcaataataa tttttaaagc gacagattgg acacgtcctg cacttaaccc tttcttgacc
    20461 ttacgccaga gaataggact gatcgaataa ccaacgattc tgtctaaggt acgacgagct
    20521 tgctgtgcat ctactaaatc tacatcgatc gttcttggtt ccttaaaagc tgctttgact
    20581 gcgtcttttg tgatttcatt gaagaccacc cgattttttt ctttcagatc caacccaagt
    20641 aggtacgcta aatgccaagc aatcgcttct ccttctctat ccgggtcact tgccaagtaa
    20701 actttttgag ctttttttgc tgcggctttc aggcttttga tgacatcgcc ttttccgcgt
    20761 atagaaatat aatggggttc ataattgttt tcaaaatcga tacccatttt acttttcggt
    20821 aaatctctta tatggccaac actggccatt actttatagt ttcgtccaag atatttctca
    20881 attgtcttgg cttttgcagg tgattctaca atcactaaat atttatacgc cattactgaa
    20941 tggactcctt tccattgtcg tgttcctact attaaaagag gattcataca aatcgtagct
    21001 aatcatatcc attcagaaaa cgtttgtcaa gaaaagcaaa tgaaaagtat ggtgttattc
    21061 tttaaattct gccaagatat cttcacccga aaccgtgcaa actgcaccgt cttggatcaa
    21121 atgatgacat ccggtagaat gttctgttaa aatatttcct ggaacagaaa aaactgttct
    21181 tccaagatcc aatgcttgct gtgctgttat cagagagccg ctttttttct tggcttcaaa
    21241 gacacatgtt cctttagaca aagctgcgat aatccgattt ctcattggaa aacaatgctt
    21301 tttcgggccg gattgaggag gatattcact caaaagaaga tgatcatttg caatcacctc
    21361 ttgcaagtca cgattttcag caggatagac ttgctctatc cctgttccaa tgactgcgat
    21421 tgtccgtcca ccatgagaga ttgctgcttg gtgaccgtat gtatctatcc ctttcgcgca
    21481 gccactgacg atcacatatc cagcttctat cactggtttt agtaaataat aaactgtttt
    21541 tttggctaaa gcagaagtgt gtcttccacc aacaacgctt aacatgttac aagaaagtaa
    21601 agatagatca ccattataaa acagtacaat tggcggatag gtcaagtgaa gcagctgagg
    21661 gggatatttg ggatcaaagc aagtgataaa cgactgtttc gattggattt tgccccagtt
    21721 agcgtgaatt tcctgccaac ttttgcgaaa cagctcccgg tgtttggtta ttttacctat
    21781 cttaatgatg tcttcaaatc cgatttcttt ataaggatac tgcatcatcc agttagcaag
    21841 tgcccatttt ccttgactac tgattccttt tgtataagca agtttcaata aattttcttc
    21901 tatttgatac atcataaaac cgcctttcgt ctctctttct aataagatac gtaaaagcgg
    21961 ctgtttttta ttgtaaatat ttttttacag gtgcaaaact tttacgatgg attggcgtaa
    22021 ttccttgtcg ttcaagtcct tctaagtgtt cctttgttcc ataacctgca ttatgcgaga
    22081 aaccatatcc aggataaagc acgtcatatt ctttcatcaa ttggtctcga tatactttag
    22141 caatgatact tgcagcagca atcgacacag atcgtgcatc ccctttgatt attttttcct
    22201 gctgaatagg caaatccaag gtcatcgcat cgatcaacaa atagcttggt acaatggaca
    22261 atttttctac tgcttggatc atcgctaatt tacttgcttc ataaatattc acatcatcga
    22321 ttgtctgctg atcaacgatc ccgatcccga ttgccagggc tttttcttga acttgttgat
    22381 aaagaagttc tcgctttttc tctgataatt gtttggagtc attcagccct aatatgatct
    22441 catttttcgg caagataact gcagctgcca cgacaggtcc agctaatggt cctcttccta
    22501 cttcatcaat gccggcaatc cattggtgac cttgtgcgta cccttgcttt tcaaagcaaa
    22561 acatttcttc atatttttcc ttaattaatg cttccttttt caatcgtcgt tcaaaagaag
    22621 ccagtgcttg ctgcacgcct ttgcgtggat catttcttaa tgattctagt gtaggatcat
    22681 ctattctctg tatgcttttc aatcgttctt tgatactttg gatggattca gtcatcactt
    22741 ttttgctcca tttcttcgca acgatccagt gtgtatcgtc caagttttcc ttgacgaata
    22801 tcatgcacga ctaattcgct gccacgatca tagtcttctt tatacccacg ttttgctgta
    22861 atatccatca gtaattccgg tgccaatttt tcttcttgct cgattgataa accataacga
    22921 tcgctgattc ttcctgaata atatcgggca aaaaagtcta atccgtaaat cgctaaatca
    22981 tctaaatgga caagctggtc tttgatcgcg cctgtcagcg ctagtttttt gccgatttct
    23041 tcatcttcaa atttgggcca taaaatccct ggtgtgtcta acaattccaa ctcattacct
    23101 aaacgaagcc actgctgacc ttttgttacg ccaggcttat ttcctgtctg tgcaattttc
    23161 ttgcctacta gtcgattcat caacgtagat ttcccaacat ttggaatacc taagaccatt
    23221 gcccgaatag cgcgaggctt gatcccccga gcagcatctc ttgcccgttt ttctttcaat
    23281 gcttctttag ccgcaggtac tagaccttta attcctttac tttcttgtgc cgtgatagct
    23341 aatgcaagat ggccttgttt acgaaaatat tcttgccaca gctttgtctg ttgtggatct
    23401 gccagatccg ctttattcaa taaaacaagg cgaggttttt gctgaatgat ctgatctagc
    23461 attggattac gagaagataa aggtaatcgt gcgtctacca attcaaatac gatgtccaca
    23521 tatttcattt tttcagaaac ttctcgtcgg gcttttgcca tatgtcccgg aaaccattgg
    23581 atggtcattg ttctctccat tcctttcata ccggaatatt ccccggcttt acttgttttt
    23641 ttctttttca atataaattc acttttttat tataaagaaa ttccatctat tagttaagtg
    23701 aaagtgaacc agtcctcatt ctatcacatc ttcacttttt tgatgatgca aatttttatt
    23761 ttggtttcta tatatgaatc taaatttttg acagcgtttc ctaaataaga gatgatagac
    23821 gaacaaaaca gaaaggagac gaaacaaatg aatagtatgg tttttgttaa ttttcctgtt
    23881 gctgatgtca atcgttccgt tgctttttat gaaaagctgg gttttaaaaa aaatgaagaa
    23941 ttttctactg aagaagctgg ttcaatggta tgggatgaca ctttttggat aatgcttctc
    24001 tctcatgatt tttataaaaa atttataaaa gataaagaaa tagctgataa taaaaaaaca
    24061 agtggtgctt tgatttcgtt tagtgttgaa aatgcggatg aagtaaaaaa atttgctcaa
    24121 attgcaaaag aaaatggtgg tactttccac gaagtagata tgggaatgcc tgaagaagaa
    24181 atgttttcat tagaagtcca agatctagac ggaaatacat tagaacccgt atggatgaag
    24241 atgtaagcag ataactaatc tgacgttttt tataacaaaa aaacaaaggc ggatgatacg
    24301 ataagtattc tccgcctcta ttttttttat tcgtacaatc tcttttttac agtttataaa
    24361 agcagtcttc tcaccctttt tttaattgtt agatgccagt gtatgtaaat caccaatgtg
    24421 tcatgacaca ttggtgattt tatggtatcc ttttattatt aactgacaaa ggagagaata
    24481 ttttgaacac tttgattgca aaagacgttt ttagattgcc cgaaatgaat gcaagattgt
    24541 tgtcgacctc tgctctttta ttagcccttg atgtcatctt atacaagata gcgattggtc
    24601 ctgcttattt tcaaatcaca ttcggatttt taagtatggc gcttatgggt tatctatatg
    24661 gaccgatctg ggctggtttg cttgaaatgt tgtcgaacac tttaaatttc acgatatttg
    24721 gttcaggaac gtttcaagct gcttttctta tcacggcttt ccttggtgga tttattaatg
    24781 gcttattttt atatcataaa aagataaacc tgccttccgt catcgctgcc caattattga
    24841 ttatgatccc agtcagcctt tttatcaatt cttggctgat gtctgttttg tttggtgcag
    24901 atttcaaagt aatttttcta ggtagattca tcaggaatat gatccaaatc cctattcaaa
    24961 taatggttgt ttattcattt atcaaaactt tagaggctag aaaagtgttt aaacgagctg
    25021 tttgatttta cgtactaagg tagtaaactg attttggatt aaacaataaa gcgtgcagtc
    25081 tcaacagact gcacgcttta ttgtttctca ctggatccat tttatttcat caagtggata
    25141 atagacaaat cttgctttac ctagaatatc agttttattg atcgtgccaa acgaacgact
    25201 atcttttgaa actcttcgat tatctccaag tacaaaataa ttatcttccg gaagcttgga
    25261 ttgtcccgtt agctcttgta gtgtaaagtc cgtcgtatag gatgcatttg catgatctga
    25321 tttcaggttt ttagttagat aaggttcctt aactactttc ccattgatct tcaattgatc
    25381 attttggtag ctgacatttt ctcctggaag accgattact cgtttgatat aaatcgtgcc
    25441 atctgctaat tgaaaaacaa caatatcaaa tcgtctgatc tccgaaaatt tttccatcac
    25501 aaccatatca ccttgcttca acacgttttc cattgaattg ccttccactg gtaccgggat
    25561 gaataaaaat ccccgcaaca tgaacgcaag gaatacagaa acaagtaagt atttgaatac
    25621 aagccagaag cgattaatat agcgttgttt ttttgtcaat ctctatcaat ccatccctct
    25681 ttttttcatt ctttctttat tataagaaaa atatcaaaaa aatgcgacta gatagctgaa
    25741 ataaaaataa ttctttcaat taggctattg gacaaaaaac ttttttgatc ataaagtaca
    25801 aaactatctc ttatatgtca tgattatttt ctaaatattt ttcccatgct tcgtcaaaga
    25861 tatccatgga cggcaaatag tcgacattca gttccagata attagaaatt tcttcgtagc
    25921 tagagctgtg cttaggaaat tggatgtctt taccggcttc tgtagcgaaa acagataaag
    25981 catcatgtgt gccaccgcct cgcagtgtca ttaaataatg aaaaaagctt cgattcaccc
    26041 ttcgttcctc caattttctt cgataaaggc agcccggcgt gcatggtttc ttttaaaatt
    26101 ttcaggatgc tttttataat actcttgatg gtcttcttct gctagataaa atggttgggc
    26161 tggttcgatt tttgtaacga ttggctgttc gaaacgtcca ctttcagcta actgtttctt
    26221 acttttttca gcaatgatcc gctgttcttc tgtcaaataa aagatcaccg ggcgataatt
    26281 gtcaccgcga tcctcgaatt gtccgaaagc gtcagttgga tcagtttgct gccagtaaag
    26341 ttctactaat tgttcatatg aaatcaatgt tggatcatag ataatctcca ctgcttctgt
    26401 atgtccagtc gtttggcttt ttacttgttc ataagtcgga tctggtacat gccctccagt
    26461 atatcctgac atcactgtat ggataccagg aagcgtgtcg aaaggctgga tcatacacca
    26521 gaagcagcct cctgcaaata ttgctcgttc cattatgatt ccccctctaa tactactcat
    26581 ccgatgattt tttattttct tttgtcttgc tttcagtact gctggtgcta gttttgttgg
    26641 tatttgagtt gtttgaagca tcagaggtat cttttggcaa atatagacta aaccgaatgt
    26701 catcatttac taagtcgatt ttatctgctt taatatacat cccgttttgc attcggaatt
    26761 gatccaacct aagcatgata gtcagatcat ctgtattgac ttcgatccaa tctggtaatt
    26821 tatagtttct cttgatcata ttcatcacat ctttgattgg caaactcaat gttccgatag
    26881 aaagacttct agcttttagt tggacatttc cgttttccat cacatacgga tcaaaatata
    26941 aatagaaatc gataggaaaa cctagtactt cgaaagtacc attgagtagt gcttcatttt
    27001 ccaaataaaa tttatattca atatcactat ctttttgata ctcactaagg aaaaagctga
    27061 tgatttgatt gactttttct ttatttgaat tgatcgttaa gacaggatct ccttgtcttt
    27121 ccgtaagagc tggtatttct gggagttctg gttcccgatt agcaaaaata cggctgccta
    27181 aaaatacggt cgaaccaagt actaccccaa gaaggataag gaaggcgaat ttccagtaat
    27241 ttgttttctt tgttttgttt gaagatacgt cagatcttgt tttcatgttt attgatcatc
    27301 ctccttctct aaccattggt tttgtgtctc gatcagctga tctctcactg catttgccat
    27361 caattgataa cctaaattat ttggatggaa atgatcttct tcatagagca cattattttt
    27421 gatgccattt agattttctg tcgtgctagt tgtttgatct gttccttctt ctgtgacacc
    27481 tacttcctga ttcaatcctt gataaagcaa atcattgatt gggataaagt agcagttcgg
    27541 catttttttt gtcaccgttt ctgttccttt gttccaattg ttaacaattg tctgcatgtc
    27601 agtgatctca ggaaagttca aataaaatgg attgtagatg cctaaaatgt aaataggtgc
    27661 ggttttattg agggatcgaa attcttccaa tagttctgtg acattttcct gatagttttt
    27721 cagtggcttt ttaaaggaat tgaccgttaa cccgaataaa ttattttgga taactttcat
    27781 caaatcatta ccgccaacag tcagtgtgat aaaatcagct gttttgatat tgtttcgaat
    27841 ttcttctttt gtttttactc gctttaagat ctggtcactt cgttcaccat ttacaccata
    27901 gttttctgtt tcaaccgcgg tcaaattata acggtctttt agatcatttg cgacaagggg
    27961 aacgaatcct ccttgcttgg tttgatctcc tactccttca gtcagcgaat ctccgatagc
    28021 tatgtagtga agtttttctt ttttatttgg atcttctctc acagtagtta ctggtttcat
    28081 tagtgggcca gctttcggta tagaagcagc taaacctgca tagatgatga tcgcaattaa
    28141 taatgggaac aaaacgagga tcaaatgtcg tttcgttaat ttcatttttt cacctcttgt
    28201 attcgattgg ttctaatgtt atcaaaaatt ttatcaaaaa ggtagtcttt atgaaaaaag
    28261 aaaacaatta ttttcctaat tttataaaga aaaagagaat aagctgcaag cctttcaggc
    28321 tgagggctta tcctcttttc gactaatcta cataatacat gattgcaaac gcgtctttac
    28381 cagtatgcgt agcgataacc ggattcgtat gcagtacagg gatatgcatg tccgggaata
    28441 attcttgcaa accctctttg aattcttgcg ccaattcttt tgcatccgca tgtgaaatac
    28501 cgatttgtcg cacatttggc aattctttca gttcattttt caaattatta aaccatttat
    28561 cgaacgtttt ctttcctcgc cccttagtta cagggattaa ttcagaatgg gcgaaattca
    28621 tcaccacgcg tatgttcaaa acactagaca gcaaaccagt tgttcggctg attcttccac
    28681 cttttaccaa gttatctagt gtggatattc cgatgtataa ttttgtattg tcttgtactt
    28741 ttttgacttc ttcaaggatt ttctctactg ttttaccttc ttttgctaat tcagctgctc
    28801 gaattacctg aaaagataat ccttggtcag tggtatcact gtcgataacc gttactttcg
    28861 tttttgttag attactggct tgtcttgctg cttctactgt tccactcaag ccttttgtca
    28921 tatggatcga aatcacttca ctgccgtctt ctcctagctc atcatacatc gagatgaatt
    28981 ctccaattgg tggttggctt gttttgggca gattttttgc tttcgccatc atttccataa
    29041 atctttcgcc gtctagttgg tcatcatcgg gatatactgt tccgtcgatc atgatggata
    29101 gcggcatgac acgaatgcct aaagcatcgc gtacattttt ttccatcgta catgaagaat
    29161 ctgttacgat ttttacattt gtcattattt tctctctctc tttctttaac aagatgcctt
    29221 attcatgtta aactacatgt aagacaattt ctatttttag tataacagac aaataatgat
    29281 ttttcattaa ttacacctag taaaggagcc gttttcatgg aaaaaacgaa atttaatcga
    29341 acgtatgaaa tattaaatga agtgtttaat gcagtcaccc atggaattgg gactggattg
    29401 agtatcgcgg gactagtggt tcttttagta aaaggtgccc acttgcattc ctccctccat
    29461 attgtctctt atgctatcta tggatcgatg atgattcttt tatttttaac gtctactttg
    29521 tttcattcat tgatttttac aaaagcaaaa aaggtgttcc aagtattcga tcatagttcg
    29581 atttttcttt tgattgcagg aagttatact cctttttgtt tgctgagtat ccgaggatgg
    29641 ctaggttgga ctttatttgt attaatctgg ttgtttgcga ttcttggcat tatatataaa
    29701 gcgttgacgt tgcataaacg ggagagagta tcaaaagtat ctacgatcat ctatattttt
    29761 atggggtggc tctgtgtcgt ggctgctgtt cctctttatc actctcttgg aatgactggt
    29821 gttattttga tggtagcagg cgggctttct tacacgatag gcgcattctt ttatagcttg
    29881 aattctatcc gctatatgca tgttgtttgg catttgtttg ttatgttagg tgcaggtttt
    29941 atgtacttct ctgttttatt ctttacttaa taaaaaaaat aaagctatgg atgggacagc
    30001 tctaaataac agctagtcta tccatagctt ttttatatcg tcaattcgcc tgtcccgctt
    30061 tttacaggat tctcattacg ctgatacgtt tcaaattgat gcggataacg atttttttca
    30121 tccatttctc cttgactagt attgatcagt gaccaatcag accagtcaac aggtggaaaa
    30181 taagcatctc catcgaatac atgatggatg actgtacggt ataatatcgt acaaaatggc
    30241 agaaattctt gataaacagc tgaccctcca gcgatgaaaa agataccgtc tgtttctttt
    30301 tccagagcaa gcacgtcttc cacactatgc atgacatgca cgccttctgc tttataggaa
    30361 gaatctctcg tcaaaacaat cgtctgacga ttcggcaacg gacggctacc catcccttcg
    30421 aatgtttttc gtcccatcac tagtgtattt gcttctgtca tttgtttgaa gaatttcaga
    30481 tcatttggca aacgccaagg aagacggtct tcttttccaa tcaatccgtt ttcatcctgt
    30541 gcccaaattg cagctaacat tttctcatcc tttctgattc taaacagcga taggagcttt
    30601 gatcgccgga tgtgggtcat atccttctaa ttggatatct tccatctcaa aatcaaatac
    30661 tgactgcttt tcctgattca aaactaaagt cggaaatgcg cgtatttctc ggctcaattg
    30721 ttctttcatt tgttcgatat gattgatata aagatgagca tctcctaatg tgtgaacaaa
    30781 ttcgccaacc gctaaacctg tttcatgtgc aattaggtga gtcagcaacg cataacttgc
    30841 aatgttgaac ggaacgccta gaaacacatc cccactgcgt tgatacaatt ggcagcttag
    30901 ccgtccctct tgaacataaa attgaaacat cgtatgacaa ggaggcaaag ccatgctagg
    30961 aacatcttcc ggattccatg cagaaacgat caatcttcta gaatcaggag tctttttgat
    31021 tgcttcgatc acttgtttga tttgatcgat aaagccaccg tcttttgttt cccagtgacg
    31081 ccattgatag ccgtagatgt gtccaagatc gccgtatgtt tcagcaaagg aatcatcggt
    31141 taggatcttc tcgcaaaaaa gcttgctctg ctttttatat tcttcattga attcaggatc
    31201 tgtcaaacat ctatggccaa aatcagtcat atctggtcca ttatagtctg gacttttgat
    31261 gtagcgttca aatgcccatt catcccaaat atggttttta ttttgtaata gatagcggat
    31321 attcgtatct ccttttaaaa accaaagcaa ttcgcttttg atcaatccaa aaggtactcg
    31381 tttcgttgtt aacaaaggaa accctttttg cagatcaaac cgcatttggt atccgaataa
    31441 gcttaatgtg cctgtgcctg ttctgtcttc tttacgatgc ccttcttcca acaattttct
    31501 tcctaagtca agatacgctt gttccatcct ttttcctcct aatcttctac ccattcactc
    31561 aagtattccc accgtgccat tttttcttca agttgtgctt ctgtttcaga tacgtcgttt
    31621 tgcaattctt gtaatcgagt gaaattatct ccttgatgat tcatctcttc ttggagcaat
    31681 gagatttttt cttcaagttc tgcaatctca tcttcaatcg tttcccattc tttttgttcc
    31741 atgtaagata atttcttctt ttcagcaggt tccttgctct gtatttttgg tttcgctttt
    31801 tcagcaggct gctgggtctc tttttgcttc agtaaataac tggacatgtt tccgtaataa
    31861 agttcttgtt ttccgtttcc ttggaatact aataacttat ctgctacctt gtccaagaaa
    31921 taacggtcat gggaaacagt gatcacagca cctggaaaac ttctaaaata atcttccaaa
    31981 atcgtcaatg ttgctatgtc caaatcattg gtcggttcat ccaataataa aacattaggt
    32041 tgctgaatca gcagtttcaa caaaaataac cggcgttttt ctccgcctga aagtttgcct
    32101 atcagtgtcc catgcataaa tcttggaaat aaaaatcgtt caagtaattc tgctactccg
    32161 atttgactgc catctgctgt ttttgcttct tcagcagctt cttgtaagta agcaatcatc
    32221 cgtttatctg gtgccatttc ttcattttct tgtgtataat acgcaagatg aaccgtttca
    32281 ccgatagaca ttgtaccgct atccagggga attctgccag ctagaatatt aagcaatgtc
    32341 gattttcccg caccattttt accagtaata cctagtcttt cccgagattg aatcaataaa
    32401 tctaaatgtt ctagcagtgt ttggtcattt atacggtaac tgccatcttt gatttccagt
    32461 acttttttcc ctaaacgttg tgtcgctagg ttcagctcta aatcatcttc ttggtttact
    32521 tggtacaagt tctcctttag atcttcgaaa cggttgatcc gtgcttgctg cttggtgcca
    32581 cgtgcctgta cgccagccct catccaagct aattcctgtt tataaagccg cttgcgcttt
    32641 tcttcttgtt ctacttctac acgatcacgt tctgcttttt ctaagacgta agcttcataa
    32701 tttcctttat actcgtataa ttttccaaat gaaagttcga agattcggtt tgctactcta
    32761 tctagaaaat aacgatcgtg ggtaaccatc aatagtgcgc cacggtattg tttcaaatag
    32821 ttttccaacc actcgatcgc ttcataatct aaatggtttg ttggttcgtc tagtaatagt
    32881 aaatcgggtt catcaatcaa cacttgcgcc agactaactc gttttttctg accacctgat
    32941 aaagtactga tttttttatc taattcactg attcctaatt tttgcaaaat aatctttgcg
    33001 ttggtatctg tagtccaagc atcttcttta ttcatttgtt cttctgcttt agcataacgt
    33061 ttttgcacat cagggtcttc tccgttttcc cctagttcga tcaatgcttc ctcatatgct
    33121 cgaacagttt ggatcaaggg gctgtttcct tgaaagactg cttggagaac agttaattca
    33181 tcggaaaatt cagctgcttg agaaagataa ccaatacggt aatcagttgg ataaaacact
    33241 gtttgccgat caccgtcgct gctatcgata ccggcaataa tatttaagag acttgtttta
    33301 cccgtcccgt tcgtcccaat caacccgata cggtcttttt cgtggaccaa aaaagaaatc
    33361 tggtcaaaca aatctttttc accataagtt tttttgaggt cagttacttt taattctttc
    33421 aacatgttcc ctccttatct tggatattgt atcataaaaa tagtgaactt caggaaaatt
    33481 tttttggtat ttgttctttt tgtgttctca aaaaaagaac aggaccgatt cgatcctgtt
    33541 ctgatatgtt ttgtttttta taaaacttga aaatttttac ctttttattt tattggactc
    33601 gttttttttg actttgctcg aaacgcaaat cggaaagaaa agttatcaga tggtttgttc
    33661 atcgtataag attctaacaa attgatattc ctgcgatagc tgttcgcaga aaacgtcgtg
    33721 attttttcag caacttcgta ttcatcaatg atttttcttt caagataata acctcttagc
    33781 atttttttcg taaacagact atcttgctgg atcatccacg aagaaatcaa attccccgag
    33841 ttcattttta ttccttcaag tttgcgttgc tggataaaaa attgaaccat cgtttcatca
    33901 taaatatcag tcaagttatc taaactttca ataatcaact gagtattttt ttgatatgtc
    33961 tctctgattt cttggatatc ttcatcacta ataaatgtct gacgatcagc atatcttctt
    34021 tgccaaaaca ttttcggatg gagaacgatt cgcattaacc tacggacaac aatgaaccaa
    34081 aagccgataa aagacaaaat ctggcttgtg atagaatgtt cgaatcgaga aagaaaacgg
    34141 gagtaaaaac gataaccatt agaagaaagc ttgcccgaac gataactttc atccaatcca
    34201 tcccgttcga tcgacaaaat cagtgcttgt atttcttgta cttcttgctt ttcatcatcg
    34261 gttaaatctt ccaagtacag atcacgtatc cgttcttgat aattttctat cactgcttca
    34321 gtggctaaaa attcttttgt attacgatca atttccttta tttcttttcg caaaacttca
    34381 acgacctctt ctaaaatcat caaagcattc aaatccgtgc ttttcgcttc ctctgtttcc
    34441 gtcagcatag gcaagacaat gatccctatg accaaagtca ctaagatgac acccgccgtc
    34501 agaaacaaaa gcaaagaccg ctggtaaaac atcatattat tgattgaaaa tggcaaaata
    34561 aaaatcgtag ccaaactgac tgttcccttt acaccgccaa atgttaagat caagatctcg
    34621 ttcaactgtt ttttgaactt tttactgcca tctttgaata cataaaaaag actgatagaa
    34681 atgaatctga tcacaaaaag catgacagag attaatacga taatcgctaa taaaagaccg
    34741 tttggatata aaccgtctcc ccatactggc gagaacactt gcgtcaattc gattcctaaa
    34801 aatataaata ctaatgcatt cagtgtaaaa gcaatagttg tccatgtact gtgggacaag
    34861 ctcgacaatt ctgcatcaaa cacagagatt ttcctaaaac cagaagcttg gagaatacct
    34921 gctgccactg cagcaatgat tccagataca tcaaatgcct ctgccaatac ataggctaga
    34981 aaaggaagca aaagttcgat cagcagatac cctgtgacat cttgtgctga tgctttttcg
    35041 atcaaatgaa ttattttttg tttgaaccag acaagcaaaa agccgatcaa agctccccca
    35101 atactagaaa ctactagact catccccgcg tttactgctg aaaaagatcc ggtgatcagc
    35161 gcaccaagag caaactggaa ggcagtaaca ccagatgcat cattcaaaag accttctcct
    35221 tccaaaatat gcatcgcctt tttagggata ttcactcgtc cagaaagaga actgactgct
    35281 accgcatctg tcggacctaa cgctgctcca aaagcaaagc aggcagcaag tgggacactt
    35341 ggcaacagga atgataacgt cgcacctacc ccgacaaggg tcaaaatgac tcctccaaat
    35401 gccaagaaca aaattgtccc gaaatttttg agaatcgaag aaatatctgc tttttctcct
    35461 tctctaaata acaatggtgc aataatcata actaagaaca attcaggttc aaaatcgatt
    35521 gattgtcccc actctgtcag acctaatacc actccaagga aaatttggat cagtggagct
    35581 ggaatcattg gcaaaatgcg gctagctatg ttcgataagg tgatcgttac tgcaaataca
    35641 ataactaact cagcaaattc catcgtccat cccctttatt ttttattttg tgtaagcatt
    35701 tttaaaaatt gttctttttt ctttaaaatt ttggtttcga ctacttcaac ctgtgtttgg
    35761 ttcccctcta attcacactg tacacgtttt gactccaaac gaagcagatc atgtttcaga
    35821 cgttccaact gacgatacat caatatattt cttttacgca acttacaaag tatttgacgg
    35881 cggtcatcca tcgaaagggc gccatagcga tcatgcaaca gttttttttg ttgttcaaaa
    35941 ctctctctca ttttccagat ctcctataca caaacttcca tcattatagc aaagttcttt
    36001 ttggatgaaa aatatttcat tgtttcatcg tttattgcta gttagaaatt tccataaatt
    36061 tttcaacgcc tcattctatt ccaaatagta tcagatatca agtaaaactt aagaaagata
    36121 aaagtttctg acaaaaggat gtttactttc ccattcctgc ctttcttgtt cttttatcac
    36181 aacaaaaaaa tggggctgcg acaaaaatct tgtcacagtc ccttattctg aataaacggt
    36241 gtggaagaga cagcctatca gtagtaataa gacacatctc tttcacagcc tcatttttgc
    36301 gtaaaaatcg atttattcga aaaattcccc gtatacatct tctacacgtt gatctggatt
    36361 gatcacgata tacaagttac ctgttttttc gctgattttt gttgattgat attgattgtc
    36421 taatccttct gttcctttat ctagatatgg gaagtacaca agatcaggtg tgccatttcg
    36481 gtaaaggatt tccattcgct cgatatcttc gtatttcaat gcgcgattaa acatgcccat
    36541 ttccagtccg cctaggttga tatcatgtgt cgcaactgcg tccccttcat ggaagatttc
    36601 gattttaaat ccagcacacg gatggatttc aacaaattca ctgccattga ttcgtccaaa
    36661 acttgttgtg atttgtttga tccaaagatc gccgatatga cggcggttga tcgtccaggt
    36721 ttctccattt ctaaaataaa attttagcgc tgtcatttct ttactcatta aaaagactcc
    36781 tttatgatgt aatttattct tgtactacct cagtttagca caaatttatg taagtggtca
    36841 catcttatgt tttttcttct aatgacgtat ctgcccactt taagtagcca acaaagctga
    36901 gcagatacag tctagtgttt tttaaatcaa tttttctttc gataaaaaat ccaaaacctg
    36961 ttttttatca ttttttattc tttgttctac gacggctttt tcagctaatt gcaaagcttc
    37021 actaacccac ttaccaggtt tttttccagt ttcttctagc aagattttac catctactgc
    37081 taattcgctt aatgacttga taggtaaacg atcataggat tctcctacgg catccatctg
    37141 aggtgtttcg tcaaaataga tcatcaattc ttctacttgc agtgcatgtt ttttgcctaa
    37201 gaagtacaat tcgaatggtt ccaaagctcc agtttctcgt tgtcgtagtc cttggacaag
    37261 tgcttgaacg tctttaatca tttggttaga acacttccac gatttcaaaa aggaacgaat
    37321 ctcattttct tttagatcca gttggctgat caataatgcc catgcttggc tctcttcttt
    37381 caatgggacg gatggtaatt cagcaaaacg taaaagtgct tcgccttctt ctttcaaacc
    37441 gggacagcaa atgtagcact ctgtttcaat aaatgcatgg attcccgcgg cccgttcatt
    37501 tcccaagagc atcttgatga attccacatt tatacgttct acagatattt tacctaatag
    37561 ctcgtggttt tctttgatgg cgtagaaggt ttcttcctct aatttgaacc ctagttgact
    37621 gacaaaacgc aacccgcgca tcattcgtaa ggcatcttca tggaaccgct catgaggatt
    37681 gcctacagct cgcaagatct gttcttctag atcttgcaga ccatcgaaca aatcgatgat
    37741 ttctccatct tctttcaaag caaacgcatt gatcgtaaaa tctcttcttt tcaaatcttc
    37801 tgccaacgaa cgaacgaatt cgacatgatc tggtctacgg tagtcctgat aagttgattc
    37861 cgtccgaaat gtggtgatct catattgttc atcattttct aataccaaca cagttccatg
    37921 ttcgatccca atatctatcg tttttggaaa aagctgtttg atttcttctg gaaatgcgct
    37981 tgttgcgata tccacatcat ggattggctg gttcagcaag atatcacgaa cacttccacc
    38041 tacaaaataa gcttcatatc ctgcattctc taattttttc agaactggaa ttgccttttg
    38101 gtattccaga ggtaattgtt ttaatttcat ttttccaacc tttcctgcgt gtttcttgtc
    38161 ctgagataaa gactcagttg catggtgact gtttgacaag tccgtttcag agtcttcaag
    38221 gccaggacca cttcatcttg tttactcgtc cttacgctca aagcgattat ggtctctttg
    38281 attgaatttt tccatgtttt tttcaaaaac ttcagttaag tcgatattca atgaattagc
    38341 catgatcatc gtaacgaata aaacatcacc aagttcttct gctaccgagt taactggttc
    38401 agaagccttt ttgctttttt ctccataagt gtggttgact tctcttgcca attcacctac
    38461 ttcttcagtt aatcgagcca tctgagctaa tggagagaaa taccccacct tgaattgctg
    38521 gatatattca tccacttcct gctgcatact gtataaagaa cgctttttat ccatcttttt
    38581 ccctccaaaa ctatcttacc aaaactttaa cgcaaatcct agataagagc aattgagaaa
    38641 atcgaatttc catgctacat ttaggaagga atttctaaat ggaaattctg tgaaaataca
    38701 taattgaaag ctggaattca tatggtcaaa cgaaaaacag taaaagaagt tctttttatc
    38761 attataggaa caagtattta tgcatttggt cttgtttact tgaacatcgc taatcattta
    38821 gcagaagggg gagtctcagg gatcaccttg atcttaagag ctttatttgg tattgatcca
    38881 gcttatacga ctttattaat caacatccca ttgattttac taggaggaaa gatcctcggt
    38941 aaacgttcct tggcctatac tgttctaggt acactttctt tgtcattctt tttatggatc
    39001 tggcaaaggg ttccactaga aatcaattta cagcatgatc tactgatcgt ttctctactt
    39061 gcgggtttag ttgcaggtgt aggaagcggg atcgtctacc gcatgggtgg aacaacaggc
    39121 ggcagtgata ttatcgctcg gattttagaa caaaattacg gcatcactat ggggcgttcc
    39181 ctattggcat ttgatatcat cgtcttactt gcttcattaa cctatatcga cttgaaacgt
    39241 atgatgtata cgttgattgt ttcgtatgtt ttcagtcgtg tgattgattc gatcttagat
    39301 ggcggatatt ctgcaaaagg tctacttgtc gtctccaaca aaagcgaaga gattgcacca
    39361 ctactgatga ccggtctgca aagaggtgtg actttccttt ctggagaagg cgcattctct
    39421 ggtaattcaa aaaaaatcat ctatatggtc gttagctcta gtgaattaac tgaagctaag
    39481 aggatcgtcc acgagattga cgaaaaagct ttcctttcaa tcatcaatgt gcatgaagtg
    39541 gaagggcaag gctttactta tttgaagcca cagacaagat tactgaaaaa actataagaa
    39601 aaaagacaaa gataggctat gaggaaagat tccacatcca gctgaccttt gtctttttta
    39661 gtactatact atttttaact aattgttatg ttcacttacc cggttacttt tcttttgtgt
    39721 ctgcaaaagg atctttcggt taacttcatc agataatttc aaaaaagcat ccctatctcc
    39781 atcttctagt gcttgatcga tttgagcata aagcattttt agttttgcct cttcttcttt
    39841 ttcttgaaaa tatcgttcaa ttaattcagt atcttcttca ctgactgaag aatcccaaga
    39901 agaaaatgga ttgtcttcta agatagatag atattctctt gtctgccaag cattttcgaa
    39961 tatacattcc acatataaag gctttttcca attcaaacgg atttcatgaa aaatctggtc
    40021 tgtgtccgta aattcttttc cctgcaaaaa caatttcatg ggttcccctt caacagatag
    40081 atcactgatc tgcagtcctc gatccgcaaa attcgcccct tcaacaaagt ggacattgct
    40141 taaaatcgct tcatgattgg caagataatt caagatccaa aaaacttctc ttcggctaaa
    40201 agatacatga ttgaccatcc acgttaaaaa attcttcttt tcccgtacat caattagcat
    40261 ttttctcacc tctctgttag gtcatctaat aatgacagtg cctccatatc tcctggttca
    40321 agtgttaaat aataagtcaa taattcttgc gctcttttca gctgcccttc ttctcttaag
    40381 aaaaaggcat actctttcaa gaaatctggt tcatgtgata gttcatgata agcctgttca
    40441 tagtgtacag ctgcaacggt aaagtcttct aattcattat aagcatgggc taggttccat
    40501 tctgcataag gattagcagt ttcttccatc tgattgatca cttgtatcac atcttcatat
    40561 ctttcatcat ccaagtaaag attacttaaa gtaagtaatg tttcatcttg tttgtctcct
    40621 acttctaaag ctttcagtaa aagttcttct gctcgctgtc tatcatgcag acggaaagca
    40681 ttttccgagg ccaggtgata aagttcgact tggtaaggat tttctttgat tccttcttct
    40741 aacactgttt gtgcttcttc aatcatttct tcttcttgaa gagcttgtcc tagatataga
    40801 tataacgatt gataatgcgg attaacttct cgaagttctt ccagatagtt gattgcctgc
    40861 tgattttctt tcaattgaag attcgtaaaa gctagctgga acagtcgatc atctgtcctt
    40921 tcttctttca gtgcttcttc taaaggtacg atagctgctt cgaattctcc ttgcatactt
    40981 aaggattgtc ctaaacgttc ctgcatcgag attcctgaaa tttcatttat cccgcttgcc
    41041 aacaatgttt cataaaccac tgctgcctct ttgaaacgat caactgaaaa atacaattca
    41101 gctagcgcaa actggatcaa tggttcatca ggaagcaacc cagctgcttc tttcaatttt
    41161 gcttcactca cttcagggat tccgataact tggtacaaat cagctatcgc caataagctt
    41221 tgcggatagt aatcactttg cttatccact ttttccaggt attcaaaagc taagtcgatc
    41281 tcattatctt cgattgcaat ttctgctaac gggatgttca gtccgtcatt ttctgggtat
    41341 tggttgatca gttgttcaaa aatctgtttt gcttcgatca aaaagccaat agccagcaat
    41401 tcttcgccta gttcagctaa tatatctgct gggtcttctt ttaatgcttt ttctaaaaag
    41461 atttgtgctt gtgacaagtc ttcatctgat aaagcttgca acattttttc gctatttgta
    41521 ttcatttcaa ttccctctta tctcactgac gatttgttga aataccgtca ttgttctcaa
    41581 taattctttt tcgtttggat gttggtatcc gtctattata cctatttttt ctaaaacaag
    41641 tatttttttg cttttttctg ccaaaaaaag aactttttcc aaatactcca tctttgaaat
    41701 ctgttccagc cagtggattg gatagccaag caagtccagc cacacaaaaa agttttttag
    41761 ttggaaatca aaaggatatg cttggagatt ccacacgaaa tgaaacatca ttcctaaaaa
    41821 ttttttcata ctagcagaaa gtagatggcc attttcggtc agataaaaag cttgttcaaa
    41881 cagtttacca taatttgaca ggttttctgc ctctaattga taaaaataga tcatttcttc
    41941 gatcatcccg gcaaacggcc gagtcgttaa ttgcttttta tttggatagt ttttatacag
    42001 attttgcaaa aaacgatgat cacaaatcat tccgcaacag ataaaagtca aaaagtcaat
    42061 ctgctttccg tccacctgcc gctcgctgat tgtttgatca taaacgatgc gttctggcag
    42121 attgatggtt tgcagcacta cacgatttgg tagctgcaaa atcgtccgct gcggtgttaa
    42181 tgctcggctt agagaacgga tcgatacagg gatcatccat agtgatgaat caagggttgt
    42241 atgtttttga aaaaaacctg ccagatccat gatcccttca tttccaaatc cgatgataaa
    42301 catggattga ttttcgggat agcgtttgac gaaatcaagc aggttcttta attctgttaa
    42361 atgattgcat tgagtgttgg cacaaatata ccagtcattg tttgcttgat ttgcaaataa
    42421 cgggttgatc ttatctgcaa acaaatcata atagcgctga ttcgttaaaa ataagattgg
    42481 ctgatcaatg gaaagttctc ttagctgact gactagcgtt tctccgtaaa acactttcgt
    42541 caacgcatct tctacttgat aatcaagttc catacgcatc cctcctcttc ttatttttat
    42601 tttaccatta gtaagcaaat tctagaaaag ataagagcaa agctcacctc ataacttgca
    42661 aataaataaa aattaaataa aatacatgtg ttttctatgt atgacatacg gattcggaat
    42721 ctctgtctct tatttattcc ttttctgcta ggaaataaaa agaaaagcac ttgtagccct
    42781 agagccacaa gcgctttttc aactgtctta tttaacagcg tcttttaatg ctttacctgg
    42841 tttgaatgca ggtactttgc tagctggaat ttcgatttct tcaccagttt gtgggttacg
    42901 acctttgcga gctgcgcgtt cgcgaacttc aaagttacca aaaccgatta attgaacttt
    42961 ttcgccgtta gctaaagcat cttgaatagt tgaaaataca gcatctactg ctgcagttgc
    43021 atctttttta gttaaatcag tagctgaagc aactttttcg attaattctg ctttgtttgc
    43081 catggataga ttcacctcct ccacaagaga tatgatagaa atatcattta gttaaaactt
    43141 tctgagtggt ccagaagttt aaaaagaaat attgattaac ttcaatattc tgttatcacg
    43201 atatcatatg aaccgcttaa tagcaaggtt tttaccaatt ttcacatgct ttttttagtc
    43261 atagaaacgt aacaaacgaa actgtctttt tcttttgaaa aaaaaacgtc gatatggtaa
    43321 aatgcctatt ttctcctacg agggataatt cggatggacg tcccttcaaa agtaaatgct
    43381 tttctgattt gattttctaa gaaacgttca taagaaaaat gcatcaattc ctcttcattg
    43441 acaaaaacga caaatgtagg tggtttgaca gcgacttgtg tagcataaaa aatcttcagg
    43501 cgttttcctt tatctgttgg tgttggattg attgctacag catccataat gatatcattc
    43561 aacacagccg aagagatacg aagattctgg ttcatactta cttgttcgat catttcaggc
    43621 agacgttcca accgttgttt agtcactgca gaaacaaaaa cgattggagc gtaatcaaga
    43681 taccggaatt catcacgaat ctcttgttcg aaatcacgca ttgtattggt ttctttcttt
    43741 acaagatccc atttattcac gacgataata attccacgac cagcttcatg ggcgtaaccc
    43801 gctactcgct tgtcttgttc ccgaatccct tcttcagcat tcagtaccat caaaacgata
    43861 tcagaacggt caatggcacg cattgcacgc atcacgctgt atttttcagt attttcgtat
    43921 acttttccgc gtttacgcat accagctgta tctatcatta agaatttctg accactttca
    43981 gaaacgaagt gggtgtcgat tgcgtctcgg gtcgttcctt caatatccga aacaattaca
    44041 cgttcttctc ctaaaatcgc attgatcaaa gaggatttac caacatttgg gcgaccaatc
    44101 aaactaaact taatcgtgtc gtcgtcctct tcttcagtct cttcagagaa atacttcact
    44161 gcttcatcta aaatatcacc aatccctagt ccatggcttc ctgaaacagg ataaggatcg
    44221 cctagcccta aggaataaaa ttcatagata tcgtttctca tttctggatt atctacttta
    44281 ttgactgcta aaatcactgg tttattactt ctgtataaaa ttttcgcaac catctcatct
    44341 gcgtctgtca cgccttcgcg tccgctgact acacaaatga ttacatctgc ttcttcaatc
    44401 gcgatttctg cttgatgctt gatctgatcc ataaaaggtt catcgccaag atcaattccg
    44461 cctgtgtcga tcacgctgaa ttctcgacct aaccattcac cttttgcata aatacgatca
    44521 cgagttactc caggagtatc ttcgacaatc gagatacgct caccagcgat gcggttaaaa
    44581 atcgtagact taccgacgtt cgggcggcca acgattgcaa ttgttggatt tgccatatta
    44641 tttcctcctc acaagtttcc aatctcactt agtttaacga atgcattgcc aactttcaag
    44701 catagattgt caattaaacg aagtattttt attccttatt gaaatactaa tcgcttttca
    44761 aaagaaaaac tgagacaaaa gcagctgctt tcatctcagt cacagataca aaatcaatta
    44821 tttttcagaa tcatcggttg ctttttcttc tacttcttct ttcaacgcat cacctaaaat
    44881 gtctcccata gtaaatccag tattttcttc tggtaattca taagactcaa cattttttgg
    44941 ttcttcttct gaagctggtt tttcttctaa agctttgata ctcaatgcaa tacgatgttc
    45001 ttctggatgg atctctaata ctttcacttt cacttcgtca ccttcatgaa gcacttcatg
    45061 tggtgtcgca atatgtttat gtgaaatttg agagatatgg actagccctt ctactccagg
    45121 gaacacttca acaaaggcac cgaagcttgt caaacgttta actgttccgt ctaatactgt
    45181 gccaactgga gcttttgtat cgatttctga ccaaggtcca ggctgtgtgt ctttgattga
    45241 caaggaaaca cgttcttgtt caggattgat agacaacact ttgacattta catcatcatt
    45301 gacagccaat acatcacttg gttttgctac atgactatgt gaaatctctg agacatggac
    45361 tagaccgtca attccgccta ggtcgacaaa tgcgccaaaa tcagtaagac gtgcaacacg
    45421 tccactgaga acatctccct catggatagt tgaaagaatt tcttttttct tttcttctct
    45481 ttcgttttcc actaccgctt tatgcgataa gatcaaacga ttttctgatg gttcgatttc
    45541 gatgatttta aagctcaatg ttttgccttt atagtctgaa aagtctgcaa caaaatgatc
    45601 ttccaccatt gatgcaggga caaaaccacg tacaccgacg tcaacgacta aaccgccttt
    45661 gacaacattt gtaacaggtg cttcgatgac cttgccctct tggaaatctc tttcgatatc
    45721 ttcccaaact tttttagcat ctaaacggcg ttttgaaagt agatagctgc cgttttcttt
    45781 atctttgccg atcgtactga taacgactaa atctaataca tcaccgactt ttactacttc
    45841 gctgatatct tctaccggca aagttgataa ttcttttgcc ggaacgactc cttcaactcc
    45901 agccccttcg attccgacaa tcacttgttt gtcttcaatg actaaaactt cgcccttaac
    45961 aacatcgcca acttttactt cttgtacgct tttcattgcg tcttccattg actggttgtt
    46021 ctcttgattt tgatcaaatt ctgtcataac acttgtcctc ctaaccaaca ttctgccgta
    46081 agtacggtac tgtttttagt ttaattgatt attcaaaaaa aataaagcga tttcgttcgt
//
LOCUS       DACCYH010000011        46057 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.011, whole genome shotgun sequence.
ACCESSION   DACCYH010000011 DACCYH010000000
VERSION     DACCYH010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.011"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            30..911
                     /locus_tag="IVY85_000594"
     CDS             30..911
                     /locus_tag="IVY85_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8043631.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            924..2534
                     /locus_tag="IVY85_000595"
     CDS             924..2534
                     /locus_tag="IVY85_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP8043632.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2611..3270)
                     /locus_tag="IVY85_000596"
     CDS             complement(2611..3270)
                     /locus_tag="IVY85_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8043633.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVALYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3553..4218)
                     /locus_tag="IVY85_000597"
     CDS             complement(3553..4218)
                     /locus_tag="IVY85_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043634.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4365..4970)
                     /gene="dhaL"
                     /locus_tag="IVY85_000598"
     CDS             complement(4365..4970)
                     /gene="dhaL"
                     /locus_tag="IVY85_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP8043635.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(4998..5981)
                     /gene="dhaK"
                     /locus_tag="IVY85_000599"
     CDS             complement(4998..5981)
                     /gene="dhaK"
                     /locus_tag="IVY85_000599"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP8043636.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(5983..6378)
                     /gene="dhaM"
                     /locus_tag="IVY85_000600"
     CDS             complement(5983..6378)
                     /gene="dhaM"
                     /locus_tag="IVY85_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP8043637.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6532..6852
                     /locus_tag="IVY85_000601"
     CDS             6532..6852
                     /locus_tag="IVY85_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP8043638.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6964..7833)
                     /locus_tag="IVY85_000602"
     CDS             complement(6964..7833)
                     /locus_tag="IVY85_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8043639.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(7929..9233)
                     /locus_tag="IVY85_000603"
     CDS             complement(7929..9233)
                     /locus_tag="IVY85_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8043640.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9230..9739)
                     /locus_tag="IVY85_000604"
     CDS             complement(9230..9739)
                     /locus_tag="IVY85_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043641.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9914..12049)
                     /locus_tag="IVY85_000605"
     CDS             complement(9914..12049)
                     /locus_tag="IVY85_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8043642.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12132..13787)
                     /locus_tag="IVY85_000606"
     CDS             complement(12132..13787)
                     /locus_tag="IVY85_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8043643.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13789..14103)
                     /locus_tag="IVY85_000607"
     CDS             complement(13789..14103)
                     /locus_tag="IVY85_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8043644.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14273..14533
                     /locus_tag="IVY85_000608"
     CDS             14273..14533
                     /locus_tag="IVY85_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP8043645.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14547..14861
                     /locus_tag="IVY85_000609"
     CDS             14547..14861
                     /locus_tag="IVY85_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP8043646.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15591..16145)
                     /locus_tag="IVY85_000610"
     CDS             complement(15591..16145)
                     /locus_tag="IVY85_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP8043647.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16271..18200)
                     /locus_tag="IVY85_000611"
                     /pseudo
     CDS             complement(16271..18200)
                     /locus_tag="IVY85_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18506..19222)
                     /locus_tag="IVY85_000612"
     CDS             complement(18506..19222)
                     /locus_tag="IVY85_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8043648.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19223..21046)
                     /gene="glpO"
                     /locus_tag="IVY85_000613"
     CDS             complement(19223..21046)
                     /gene="glpO"
                     /locus_tag="IVY85_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP8043649.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21060..22556)
                     /gene="glpK"
                     /locus_tag="IVY85_000614"
     CDS             complement(21060..22556)
                     /gene="glpK"
                     /locus_tag="IVY85_000614"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP8043650.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22692..24152)
                     /locus_tag="IVY85_000615"
     CDS             complement(22692..24152)
                     /locus_tag="IVY85_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8043651.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24142..25437)
                     /locus_tag="IVY85_000616"
     CDS             complement(24142..25437)
                     /locus_tag="IVY85_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8043652.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25769..26446)
                     /locus_tag="IVY85_000617"
     CDS             complement(25769..26446)
                     /locus_tag="IVY85_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP8043653.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26476..27567)
                     /locus_tag="IVY85_000618"
     CDS             complement(26476..27567)
                     /locus_tag="IVY85_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP8043654.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(27668..28102)
                     /locus_tag="IVY85_000619"
     CDS             complement(27668..28102)
                     /locus_tag="IVY85_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8043655.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28686..30143)
                     /gene="ascB"
                     /locus_tag="IVY85_000620"
     CDS             complement(28686..30143)
                     /gene="ascB"
                     /locus_tag="IVY85_000620"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8043656.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30140..32035)
                     /locus_tag="IVY85_000621"
     CDS             complement(30140..32035)
                     /locus_tag="IVY85_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8043657.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32093..33868)
                     /locus_tag="IVY85_000622"
     CDS             complement(32093..33868)
                     /locus_tag="IVY85_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8043658.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33918..35414)
                     /locus_tag="IVY85_000623"
     CDS             complement(33918..35414)
                     /locus_tag="IVY85_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8043659.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35608..37230)
                     /locus_tag="IVY85_000624"
     CDS             complement(35608..37230)
                     /locus_tag="IVY85_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8043660.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37558..38871)
                     /locus_tag="IVY85_000625"
     CDS             complement(37558..38871)
                     /locus_tag="IVY85_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8043661.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39116..39340)
                     /locus_tag="IVY85_000626"
     CDS             complement(39116..39340)
                     /locus_tag="IVY85_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043662.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39360..39728)
                     /locus_tag="IVY85_000627"
     CDS             complement(39360..39728)
                     /locus_tag="IVY85_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8043663.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(39924..40634)
                     /locus_tag="IVY85_000628"
     CDS             complement(39924..40634)
                     /locus_tag="IVY85_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043664.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40652..41149)
                     /locus_tag="IVY85_000629"
     CDS             complement(40652..41149)
                     /locus_tag="IVY85_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8043665.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41186..41638)
                     /locus_tag="IVY85_000630"
     CDS             complement(41186..41638)
                     /locus_tag="IVY85_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8043666.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41773..42378)
                     /locus_tag="IVY85_000631"
     CDS             complement(41773..42378)
                     /locus_tag="IVY85_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8043667.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42828..43448)
                     /locus_tag="IVY85_000632"
     CDS             complement(42828..43448)
                     /locus_tag="IVY85_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8043668.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43450..44319)
                     /locus_tag="IVY85_000633"
     CDS             complement(43450..44319)
                     /locus_tag="IVY85_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP8043669.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44342..44749)
                     /locus_tag="IVY85_000634"
     CDS             complement(44342..44749)
                     /locus_tag="IVY85_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8043670.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44768..46057)
                     /locus_tag="IVY85_000635"
     CDS             complement(44768..46057)
                     /locus_tag="IVY85_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8043671.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        12924 a         9836 c         7888 g        15409 t
ORIGIN      
        1 atatttttag aaaaaggagt ggggttttta tggcagaaat cgtcaaagtc acacatttga
       61 aaaagagctt tggtcgtttc gaagctttaa aagatgtctc tttcaccgta cattcaggtg
      121 aagtcgttgg atttatcgga ccaaatggtt ctggaaaatc cacgacgatt cggacacttt
      181 tagggattat aaaaaaagac ggcggaacgg caacgatttt tgggcaagat gtatgggaag
      241 atagtttatc tatccatgaa cgtctttctt atgttccagg cgatatctct ttgtggggaa
      301 atttgactgg aggagcgatc atcgatctct tcatgaagct tcatggaaaa gggagtgctc
      361 aaaaaaaaga tgaactgatt caacgtttct ctcttgatcc taagaaaaaa gcaaaaaact
      421 attcaaaagg aaatcgccaa aaagtgggct tgattgctgc tttggctgtt gattcggatc
      481 tctatttatt tgatgagcct acttctggtt tagatccttt gatggaaacg attttccaag
      541 aagaagttga aaaattgaaa gaacagggca aatccatcct actttcttct catattctca
      601 gtgaagtcga gcgactagcg gataaagtcg tgatcatacg cgaaggaaca ctcgatgaag
      661 aaggaacact cgatgaattg cgtcatttga ctcgttcgac catcactata gaaacagaag
      721 cagagaatag tcacttagca caattgaacg gtgtccatga ctttgtgcaa aaaggtcacc
      781 tcgctcaatt tgctgctgat aacgaagctt tcgatgtgat tttagctgaa gcgtccaaat
      841 taggtgtgaa aaaatttgaa tctattccac cgactcttga ggatttattc atgagacatt
      901 atgaaggatg aggaggaagc gaaatgactg ataaattttc acgctggtcc accctttttt
      961 ctcaaaacct tagccgtgac tggaaaaaga tcctgatatg gctagtcggt gtcactgctt
     1021 tttctggtgg ttttgtccct gcgtttgaag aattgacttc cggacaagga ttagctggat
     1081 tatacgaaac cttgcaaaac ccggcaatga ttgcaatggt cggaccaact ccagcttcga
     1141 atgctgctga gtatacttta ggagcgatgt atgcaaatga aatgttgctt ttttgcagtt
     1201 tgtttgctgc tgtgatctct gttttacacg tggtgagcca aacgagaaaa aaagaagaac
     1261 ttggtctgat tgaacttatt cgttcctttc gagtcggacg tcaagcaaac tctttagctg
     1321 tcatcgctga aagtattctg atcaatttgc ttttagcaat atcgatcagc tgtacgatga
     1381 tttttttcca agcggattcg atcactgcag aaggtgcttg gctttttggg ctatctgtag
     1441 gtagtatggg gattttgggg agtgccgttg cactgtttgc tgcacaactg atgccaagtt
     1501 cagccagttc gaatggtctg tctttagcgt ttttaggtgg actttacctt ctacgagcaa
     1561 taacagatgt agctgattcc tccctttcga tgtggaatcc tttaggctgg tcttatttga
     1621 cctatccatt tacaactaac aactggattc ctttgcttta tttgtttttg ttcagtctaa
     1681 tcttgataat cagttcattt gttttagagg aacatcgaga tttgaacacc ggctatatcc
     1741 ccgaacgtga aggaaaaccg tatgctaaac gttctctttt atccttgcgt ggtttattat
     1801 tctatctaaa taaagggatg acaatcagtt ggcttatcac ttttggtttg atgggggcgg
     1861 catacggctc tatctacggg aatatgcaac agtttttaga aagtaacgac ttaatgaaac
     1921 agatgtttat ccaatctggc gtctcaattg aaaaaaactt tacaagcacg atcatggtag
     1981 tcttgatttg tctagctgct attttaccaa ttgctagcat caatcgttta tttacagaag
     2041 aaacccacgg acgtatgaat cagctgttcg caacaaaaat ttctaggaac caactttatt
     2101 ggaccaatat gttactagct attctatttg ctatattggc aatcctgatt agttctggtg
     2161 cactagggct ttctgctttg gcttcgatga aggatgcgaa agacatggat ctattgatgt
     2221 ttcttaaagc cgggttcaat ttcctgcctg ccgttttagt gtttatcgga attagtggct
     2281 ttctattcgg atggcttcct cgaatcggta aacttgctta tatctactta gcttactcct
     2341 ttgcactcaa ttactttggc ggaattttgg atttaccaga atggttttcc aaaacagctg
     2401 tccctagctg ggttccacgg atgccaaacg attcttttga tctttccgtg ttccttacgt
     2461 tatcatgtat cagccttgca ctgttcgtcc ttggttctat tggttatagg cgaagagact
     2521 tactagaagg ataataaaaa agcatgtaca atttcatgct agcctccaaa tctgggtcaa
     2581 aaatccaaca tttggaggtc atttttttat ttacaagtct tctaactgct ctttatcaat
     2641 gatccagact tctcgattct ttctttttac ataaccattc tcctctaata atttaaattt
     2701 tctagataaa gtttcggggg tcgtaccgat aaatgcagca agttctttca ttttcattgg
     2761 caggaaaaag tgatccttcc ctgtcgcttt agctaagtca agcagataga gagcgactcg
     2821 ttcttctact ttttccatca tcaaaaaagt tgtctgctgc tgtacttttg ttaatttctc
     2881 aacgttgatt gctaaaagtt tcagactaag ggcgggataa tgcagtaaga gttccgagaa
     2941 atctttttgt ctcaatatgc aaatttccgt tttttctagt gcttcgccaa ataaagactc
     3001 atttggaacg cctagcaact ggctctctcc ctcatatccg cccggctcta ttactcgtaa
     3061 aagctgttcc tttccgctag cagataattg atacactttc atactgccgg aggccacgat
     3121 cactaattga ggttctccat tcggcgcaag aatctgttct cctttttcca catagtgatg
     3181 tttgacaagt gcgtgtactt tttcttgatc ttgctgtact aagtgattga acaaaggaac
     3241 aaatgaaaca cataaatgat catgactcat aatctctcct ccttctaaac agctatttct
     3301 aataaagaaa cataacttcc cttattccaa ctgataattt tcttttatca tagcataaac
     3361 gtcaagcgaa ccagatacca gcaaatagag tggatttctt tttgtccctt gctttatttt
     3421 ccttcttttg agaccttaag taattgattc ctatcaattt tttaaatatt tactttttga
     3481 ataatcaaaa acaaaccttc actcgcaagc cgagagtaag gtttgttttt cgcattgctt
     3541 atttcgtgtt gtctattgaa atttttccgc acctagcttt tgatatagtg gtgctagttc
     3601 ttgtaaggtt tcagcggtta tttgctcaac gtgttcgatt tcttgcggat agaagaatag
     3661 tttacccatt tgaaaggttc ggtctctcat cgcttttttc aactcttctt gtttctttgt
     3721 atcggacaaa tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg gttgcggcca
     3781 tatgtaataa tccttgatat cgactgtttt gctccactct ttcagtatcg caagcaactg
     3841 gttatacgct gcaatgctac catatcgttc ttcgctttta taatgttggt acatcaaata
     3901 gatttggtat tgtttttgat tcaacaaaac tgctagacag gcattctcgt cttgacgaga
     3961 ttcgcttcgg tacgcgctcc aaaaatgact gcgtaaattc cagccattcg tccagctttc
     4021 gattttaggt ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct ggagctgaat
     4081 ctctttccat ttttgccagt gttctttata ttctttcttg atttgttcaa tcttatctgt
     4141 tggatggctc tctttgagct gtttgaatgc gtagattggt cgatcaaaca actgataatc
     4201 tgattttcca agttccattg tttcgcctct ttccgttatc ttttgtattc tacctgaatt
     4261 ttgcgatatg gacaaataga ggttgtgaaa aagctgttct gcatcaagtc ataagaacag
     4321 ccaaacaaaa aactgcgatg agcatcaccg cagttttatt gaacttaagg ttgattgatc
     4381 acatctaaaa gcgttgaaaa taaaatggca ctagaagcag ctcctgggtc gatatgtccg
     4441 attgcacgct ctcctagata ggaagctcgt ccttttttag ctaccaagac tttggtgtgt
     4501 tcttttgcct ggtcaatgat ttcttttgtt aaattccctg ccttcaatgc ctctgtgact
     4561 ggcagccaaa catctaacat cgttttatcg tcagcttctg cctttcctcg gctttgaata
     4621 cccgctgttc cctgctcgat cacttcgcct aatttttctt gagaatcaat agaatcgacc
     4681 tcttttgtcg ctttcatcat gttcatgaat gcagatccgt ataacggacc agaagcccca
     4741 ccgactttcg atatcatcgc catagagaat actttgaatg tatcgctgat tgtttcagga
     4801 tgatcggctt gaaaagcttc ttcataagct gaaacccctc ttcccatgtt atttccatgg
     4861 tctccatcgc caatcggggt atctagatca ctgagatatt gtttgttctg attgattgcc
     4921 tctgcaaatt gcgtgagcca ttcttggatt tcttgaacgg ttagtttcat attatcaccc
     4981 ttttatcttg agatttttta ccatgaaatc gtctgtacat tgctttctaa agaatctttc
     5041 cattgtgtct ccgtcaagtc gatcagtgtt agagaaagac cttgcatatc tagtgaggtc
     5101 ataaaattac cgacttttcg gaaaactaca ttgacgcctc tttcttccaa cagagctaaa
     5161 acatcattca tgaacacgaa ctgttccatc aaaggcgtcc cgcccattcc gttgactagt
     5221 acacctgctt cttttggtaa attcgagtaa tcatctaaaa tcttagtaac aagttccttt
     5281 gccagtactt tagaaggctg catcttttct cgacgatagc ctggctcacc gtggatacca
     5341 acaccatact cgatctcatc ttctcctagt tcgaatcctg gtttccctac ttctggtaca
     5401 gttgctgctc gaagcgctag accgatcgtc ttagttgcct gtacgacctt ttctcccagt
     5461 tctttcaact cagctaggga agcaccgttt cttgctgcat cgcccacgat tttgtgtaca
     5521 aggacagtac ctgcaactcc gcgttttcca gcagtatacg tactattttc aacggcgata
     5581 tcatcatcta cgatcacact ttctacttca atatcgtcca tagcagctaa ttcctttgcc
     5641 atatcgaagt tcaatgcatc ccctgtataa ttcttaacga tcagcaaaac tccttttcca
     5701 ctatcggctt ccttaatagc cgtctgaatc tgatctggag ttggagaagt aaacacatca
     5761 ccaagcaccg ctgcacttaa catcccttcc cctacaaacc cggcatggga tggttcatgt
     5821 ccgcttcctc caccagaaac caaaccaact tgttccgctt tctgattctt ggctacaaca
     5881 cgggaagatt ctacacgatg aacaagttct ggatagcttt ttactaatcc ttccagcatt
     5941 tcttcgacaa tattgcttgg atcattcatg atttttttca atttattccc atcctttctt
     6001 tccggcattt tgtgcttctg ttaagatttg ctgagtatca tctgttatac cggcagtgat
     6061 ggctgctgca aaagctcctt ccactaaagg cgcatccacc agatgataac gttgctgctg
     6121 ctcttcttcc aacatgtctt ttgccagttc cgagttcaat accgcgctac cgatatccgc
     6181 aaaaacaaaa taactagctg catcgcttga ttcattgact gcttctacga ttttcatcgg
     6241 gtctgatccg atctcgccat ttattccgcc gagagaaaaa atttgcacct cttcggattt
     6301 ttgcatctgc tcaatcattt ctttaatacc atctgttact ttttgactat gagaaataag
     6361 caaaatactt tttttcacaa gaatcttcct ttcttttaca tagttgctaa cgattggttt
     6421 cgctttcatt aatgaacaat tcaagtatat aataaatttc taattttggg aaaatatgtg
     6481 tagtgcttct tgaagtttgt tatactttaa ttaaaagaga ggagtgatcg aatgattaca
     6541 acaacaacga acagtgtaga gggaaaagcc gtagtagctt ataaagggat cgtttttggt
     6601 gaggtcatta ctgggatcca tgcatttaaa gacttggagg cggggttgcg aaatatcttt
     6661 ggcggccgct caaaaagcta tgaaaacgaa ctaatgggtg caagagaaga agcacttgcc
     6721 gaaatggccg aacgtgcaaa agcattagga gctgatgcga tcattggcgt aaaaatggac
     6781 tatgaagtat taggcagcga caatggcatg ctgatggtca catgtagtgg aacagctgtc
     6841 tccacgagat aagtagggga catctgtttt aataactaaa gagagatggg acatgtagtg
     6901 gcactcaaga attagatttt tcagtctaac ttcgaggtac tctaccttcc catcctctct
     6961 tttttatttc tttttgcgga tgattccgta agctgctgcg actaacacac ccgcccctgc
     7021 taaggaataa ctgacagaag ctgttttttc atttgttttt ggcaaagtct gacttgttga
     7081 tttggatggc tctgtcgttt gctgaactgt ttctgtcgtg cttccagagg tcactgattt
     7141 ttctcctgcc gaatgcaaaa tatcttttat tcgttggaaa ctagctgcta agtcagcttt
     7201 ttttgcttca aaatcattag caactgtttg tgcttcgctg atctttccgt ggattgcttg
     7261 actggccgct gtgatatccg cttgagtttg acttagcttt ccagaagctg tttgagcaac
     7321 ctgatttaat tgctgattga attcttttgc ttgttggatc ttcccgtcaa gatctgtttg
     7381 gacagcttcc gcttttgctt tcgcttcggc aagctttttc tcaagttgtg cacggtcatt
     7441 tcccacttta tttttaagat cagctaaatc ttgatcgatt gctgcttggt cttctttcaa
     7501 tttgtttttt gcagcttcta atgcttcttc gagttctttt cttttatttt ccaagtcttg
     7561 caaatgatcg atttggtcag aagaggtaga actatttatt gatgtatctt ctgaagatga
     7621 aatcgtttct cctgaattat ctgtcggtac attggaagtc atttctgttt ccgaggcacc
     7681 gttttcagca ccagaagagc ttatggttgt tgaaggttct gtttgagcag gttcagaagt
     7741 ttccgcaaaa gctgtctgtc ccaataagaa aatactggaa acagcaattg gccagattaa
     7801 cggcttcatt ctttttttca tcacttttcc catccttact actttttaac taatttctta
     7861 ttataagcaa aaatgtttgg aagaaccata aacacaacag gctgtttgtc taaaaaacgg
     7921 tgcatctttt atttttttgt acgttccggg cttttccatg tcccgcttcc ataacctaaa
     7981 atcgttttga ctgctggaat gaaagtcgtc acgatcgtta tcgcgttcac tatccagaac
     8041 aaaagcatat atagtggtgc gaaaagaagg tatttcaatt ttcttcgtcg atcatctaca
     8101 atcagcgaag ctagcaactg gaaaaatcct gcgaccattt caaagcagat gaacacaaaa
     8161 ctgaatgtaa aagtaatata aatctgttcg tagtttcctt gatatacata atgcccgatt
     8221 aatccagcaa aaagaactac ggaaatacaa aagaatattg accagacaat actcaatgtt
     8281 tgatcaatga agatgatcgt tcttccaata tgtttaaaag gatggagcat cacttttttg
     8341 aaattcgtca gccagacttc tgttccacct tttgcccaac gttttctgtg tcgatatagc
     8401 atcttcaacg tcacaggtac ttccatgaaa aagataatct caggagcaaa aacagataac
     8461 cagtcattta actgatgatc ccaagcaata ctgatgtctt ctgtcgctcg atcttgtctg
     8521 aatccgccca catcgatcaa ggcttctttt cgatacatcg tatttgctcc actgtacgca
     8581 taaagaccac cgaaaacagc tgactgtgtt cgtttaatga tgcccacgat acttgagaat
     8641 tcaaccgttt gagattttgc aatcaatttg gtacgatttt ggacatccat atttgcagtg
     8701 accgcagcaa tatgtcttgc accagggcga atgaaataat tcacatacct aatcagtgca
     8761 tctggctccg gtaccgtgtc tgcatcattg cttaaaatca atttcccttt tgcaaaggca
     8821 agtccgatat taaacgcgtg ggcttttcct ttattttttt cgatccgaac cacacgaaga
     8881 ttagcgtatt ttttcatcag tctagctaat atttctggtg tttggtccgt tgaaccatcg
     8941 tctgtcacta atacctcata gttgtgataa ttcaatttcg tcatcaaata ctcgattgta
     9001 tattcgatca caatttcttc attatgggct ggcaccatga tcgtgatgaa cggttcgaca
     9061 ctcaagggaa catctaccca ttctttttgc ttatgtttgt ataaaaacac ataacaccag
     9121 acaccaataa accatacaaa tccgcctaaa atcggataag aaacgagaaa aaaatacata
     9181 atagacaaac aagtatctaa gaataaatta cctgtatggg tgattgagat catttcagtt
     9241 ccaccccatt ttctttatac aaatctgcaa tgaatgtcgt atctagattt ttctcttcgg
     9301 agacagaata aaaacggacc gtttctctct cttcttttgt tccaaaacgt tccgtataca
     9361 cttcttcaag taattcttgt ctttttttca atttttcatc ttcatattca aaagatttat
     9421 ttactttttt gtatagattt cgattgttcc acctagtcaa tacaaatgag gtaatcagga
     9481 aactgaaaaa aatcaataca aaaaagccga ttaagaaata cagcattttc ttttctgata
     9541 aaaaagcgta caaggaaaaa tccttggctc tttctggtct cgttaatggg aaaaagaccc
     9601 atataaaagg aagaacaacg atcatccatc ctaaaagagc cactaacgtt tgccaaatct
     9661 ttaaccagta attacctttt gaaaaatact tatctttcac caaaattgat gcaacttctt
     9721 ctcttgttac ttttttcata tcctcacctc acgttttttt gttacgacgt aggagtttac
     9781 catacatttc atttcttttc acataaagtg ttcctaaatt tttcctaaga aaaagaacaa
     9841 aacatttctc atcagcaaat gaaaaatatt ttgttctttt tatcagttgt tctttttatt
     9901 ttttatctct taattatgtt gctctttcca atacgacaat gcttttcatt cctctagata
     9961 tcgccgtgta catttgtctg cgtcttcttt gctcatctcc atcgttttca aatggatata
    10021 agattacatg atcaaattcc agtccttttg ctagactcaa aggaatgatt tggcagtttt
    10081 ctaatccata tgaatttttt ttaatcgatt cttgtagcgt ttgggtttcc tcttctgttt
    10141 gggtgataac tgccgctttt tctcctttta gagaaaccaa aatctcagct agtattttca
    10201 acaattcttc agaggatcga aaagagcggt attcgggttc ttttccttgc ggtcggatcg
    10261 atacgatgtc gatcttctct tgatccacag cataggtttt gaaaagctct gtgattgctc
    10321 cgctagaacg gtaactattt ctaagatcat accgatggat cggcagatgg tagtcgtcga
    10381 aacaacgcat aatatcagca aaagtagtgg aagaattaaa gattgcctga ttctcgtcac
    10441 ctactaaagt aaagtgagtt tttggaaata attcgatcaa tagacctaat tgtgccaggg
    10501 tataatcttg tacttcatca atcaatagat agcgaaatgc tggtacattt accttttcaa
    10561 caagtaaatg acgaatagct aatataatga ctgcttcatc aacagtacgt gtgggttgcc
    10621 aagcataagc tgagccatat cgtttttcat aaattctttc aaacaactga tgctcatcca
    10681 cccatgccat ttcttcgatt tgtctggtta tttttcgata ttttttttgt aaaagtttcc
    10741 ttgcataagc tacaatactt tgttccgaat catcagaaat caatttaccg aataattcca
    10801 actgcctatc ttctgtcaac gagagtacct gattctgcac agcaggactt tttgcttgtt
    10861 tcaaaagatg attcttccat tcgctcatca gtgccttttt cgttgcttga agcctatcga
    10921 tcaatttagg atgagcagga actttctcat atattttttc gatcgttttt ttagaaagga
    10981 ctgtttttcc atttttagta agtccacgga taaaattcgg atgatctaaa aacaaaggat
    11041 ctgattgttt tagattatcg ataaactttt tgctacgcaa acgttctgtc tgctccgata
    11101 cattttctcc cgtgatacgt ttgaaataag cctcttcacc ttctatttcc tctgctgaaa
    11161 gctgtgaaac cagctgtatg atggttagat tcagtggatt tctttcacct aatgatggta
    11221 ggacattcga aatatagtcg ataaaccgat gattaggtga tagaatcagc aagtcatcgg
    11281 atgtcatcgt atctcgatat aaatagagca gatacgcaat acgctgcatg atagcagagg
    11341 ttttaccact tcctgctacg ccatttacca agatattttg acttttcaca tcccgaatga
    11401 ttgtgttttg ttccgattgg atcgtagcag tgatagcctt catttcattg gtctcatctt
    11461 ggcctaagac ttctaataat acgtcatctt gaatagctac agacgtatca aaaaacttga
    11521 ccaaacgatc tctttccaaa agaaattgtc gtctttcttt aataaccgta tcgattttat
    11581 tttgacgaac cgtataagaa gatggaccaa tcatgttatt gtaaaaaagt tcggcaattg
    11641 gtgaacgcca atcataaaca agattttcat ctgcatcatc tgtgaaattt gctgtaccga
    11701 tataaaagga ttcttttggc tcattatcca gaaagtctac aacgactttt ccaaagtagg
    11761 gacttttcaa taaacgttcg atctttgcta gctgattttc tgcgtttttc tgtttgatat
    11821 tcatctgatc gatttctcta tttttcattt caatcatcga aaaagtatcc aggttatcta
    11881 agtaactgtc aaaattcaat cgaagctcgc cagccatttc tttcaacgac tggtttcctt
    11941 ctatttttgc cgtatccatc gattcccgca gttcgttttt tttatcaatt agcttttgat
    12001 acacgttctt cagatatgct tcttctaact gtttttgtgt gttgaccata cacattctcc
    12061 tcttttttta aagtggtgga accgattagt ttacctcttt taagagtcga atgcaagctt
    12121 tttaacgagt atcatttgtt aggatatatc agttcttcag gtcggacagt ttgatataaa
    12181 gaaaatgcac catcaaggtt ttttacgtgg tagccagact gcttcaagat tctttccgct
    12241 agatagctac gtaatccgct atgacagctg acgatatatt cttgttggct gtctaattca
    12301 tttaatcggt cccgaagttc attcaaaggg atatgtttgg cttccttgaa tttgcccttt
    12361 tccaattctt ctgctgttcg cacatctaat aaaatttttc cttttgctag ttctgtcggc
    12421 aattcatgcc attgtacggt ttcactaagc ccttctgcaa tgttcattgc tgcatatcct
    12481 agcatgttta ctggatcttt ggctgagcca aatggcggtg catacgtcaa ttctaattcc
    12541 ggtaaatcga agatcgtcaa atgccctttg attgctgttg ctaaaatatc gatccgttta
    12601 tccacacctt ttgccccaac accttgtgca ccataaatct cgccagtctc tggatggaag
    12661 atcaacttca gtaaaatatc tgttgcttct ggataatagc ttgcatgatc ttttcctgat
    12721 acatgaatga cttcaaaaga cagttcgttt tctttggctg ttctttcact taaaccggtg
    12781 gatgcggcag tcattccaaa cgttcgtata attgctgttc caataccgcc ttgatttctt
    12841 cgagaaattc cggaaatcgt gtccgcaact tgtctacctt gacgattggc tggtgaagca
    12901 agagaaataa gtgcatcttt tccagtgatt tgctgtttta caacgatagc atcccccacc
    12961 gcataaatat caggaatatt cgtttgataa cgttcgtcta ctaggatacc cccgcgtatg
    13021 cctaattcga ttccagcggt cactgctagc tggttatcag gacgaacacc aatggataaa
    13081 ataactaaat cagcagggat ggtacgccca tcatccagtc gcaatcgttt cccgtggtct
    13141 tcaaatccag cgacagcatg agaagtaatc acttctactc cttgatgaga caattctttt
    13201 tctataaaag ctgccatttc ttcatccagt gttggaagaa tatgtggtgc ttgttcgatc
    13261 accatgactt ctaaacctct tctttttagg ttttctgcca tttccaatcc gatgaatcct
    13321 gcaccaacga tcacggcacg ctttggctgt ttttctaatg catgcatcac tttatcgata
    13381 tctggtacat ttctaataga aaaaacattg tcggcttctg ttatccctgg aatcgatgga
    13441 acaactggtt ttgcaccagg agataaaacc aatgcatcgt actgttcaat gtgtttttga
    13501 ccctcatgta cgacttctac cgttttgttt tcgggaaaga tggctgtcac ttcatgattt
    13561 ggacgcacat ctaaacaaaa acgagcggat aatgattctg gcgtttggac aagaagattt
    13621 tcccgttcac tgatttctcc cgaaagataa taaggcaagc cgcaatttgc aaaagaaaca
    13681 tacggtcctt tttcaaaaac aacgatttct gcatcttcca ttaatcggcg aagccgtgta
    13741 gcagcagaca ttccacctgc tacacctccg acaataacga ttttcatctt atttccctcc
    13801 tactgttcga tcaaaccatt gattcatgcc accacgtaca ttcactacat cataaccaga
    13861 tttctttaat tctttagctg cttgtttact cctcatccct gattgacaaa tcacatagac
    13921 ggtcttttct ttgtctccat catatctgtt tatactttgt aatggaacat ttttagctcc
    13981 ttttatatgc ccgccccggt attctgcagg tgttctaaca tcaagtaacg ctggattttc
    14041 caaaagcttt tcctttaatt ctgggactgt tatcgatttt attgattgaa atagcaaatt
    14101 cattttctgc acctcattta catttttata aatacctcat ggggtatatt aaacaggagc
    14161 tttattaata tgtcaaccag aatgactctt catttttttc actacttttt atatttgcat
    14221 aaaataccga taaggggtat tatctaatta taaaaataga ggagaatgtg ttatggagat
    14281 tgataataaa aatatcctga accgtttgaa acgtacagag ggacaaattc gtggtatcca
    14341 aaaaatgatc gaagacgaaa aagaatgtat ggatgtcatc actcaattaa gcgctgttcg
    14401 ttctagtatt gatcgcgtaa tgggcatgat cgttgcagat aatctgaaaa attgctttga
    14461 gaaacctgat agtaatccag aagaacaagc ggctaaacta gaacaagcga tcaagatgat
    14521 tattaaaaag taaaggaatg atttcaatga aagtcgtttt ccatattgat gaattagaaa
    14581 aatggtcaga aacagggaaa aacgtaaaaa acctgataaa agcttctcca gaaacgacga
    14641 ttgtcgtatc tgtaaatggc atcgcgatta ccggttatct agactctgcc aatgcagaat
    14701 tcttagattt gcaaggagtc gtttttcacg cttgcgccaa tgctatgcgt gctaatcata
    14761 tttcagaaag ttctttacct gaacaagtaa tcgttgtacc agcaggtgtc cttgatttag
    14821 ttgaactgca atcccaaggt tatgcttata tcaagcctta gtcagtagag gttgcgacag
    14881 aagtgatcag cttcaagaaa tttcggctta tttttcgaag aagctacttc tggaaaaccg
    14941 tgaatcaaag gttgtgaaaa cgctgtcctg catcaagtaa taagaacagc caaacaaaaa
    15001 tagcttctca tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac
    15061 cgtttaagga agaggttgtg aaatcagcgg tttgacctaa gtattagaga aaaaaacggg
    15121 aaaatagctc ctcatatttt gccgtttttt gagctaatac cgaaggtcag ctgattgaac
    15181 accgtttaag gaagagattg tgaaaaagtc gcttagtttc gagtattaag aaaaattttg
    15241 tgaaaatgga tttctacatt tttgcataaa tttggcttaa taccgagaaa ctggcttttg
    15301 aataccgttt aaggaagagg ttgtaaaatc agcggtttga cctgagtatt agagaaacaa
    15361 acggaaaaat agctgctcat attttgccgt tttttgagct aataccgaag gtcagctgat
    15421 tgaacaccgt ttaccaagag gttgtgagcc taccatttaa ctccgaatca caagaaataa
    15481 tttcaaaaac aacttctcct gtttttggtt cggttggctt attatcgaga agtagctact
    15541 gggagtctat ttatcaaaaa aaggaatgcg acataggtca cacccctttt ttattttctt
    15601 tgaaacatct ttcggtactc gacagcatac aagacaaaag attgtaaaag accgtaagtg
    15661 atgacggcaa aaatgactct cacccacagc gttggtaatt ctgggaaaag cagcaaaagg
    15721 atcatgccaa catagatgac agcagtcgct actttgccat accacttcgc tccacttaat
    15781 ttgatatttt tagatagccc gattaatcca gctacaaaca taaagccttc ttttatcaag
    15841 aatagaccaa acaacagcca aagccacggt cgataccatg ccatagacaa aatcagaaaa
    15901 agttgtgtca atttatctgc aaacggatca agcaccttcc ctaagtctgt tacctgatta
    15961 tatctccgag caatgacccc gtctagataa tcagacaaac ctgagacaac aatgaccgct
    16021 cctgctatat aaaatgactg tctcagcaca aaataaagat aaatcggcag caaaaaaata
    16081 cggaatatcg ataaaagatt cggaatcgtt tgtatttctt tacgccagtc tgtgcttaat
    16141 ttcattttat cgccctacct cctcagcact cttttctttt tattttagct tacacgggga
    16201 ataaaaccaa tgatttcact gtttttattt ttctttacag acactgatat tatgagctaa
    16261 tttgagataa tcacttgctg ttttcatcgt ttactcttct ttcttcctgt cgtttgcgca
    16321 tttcttttac tagtacttta tgagcttttt ttgatcctcg ctctaggtca cgctgccgtt
    16381 tacgtgtttt ataatcgata aacagactgt ctaaatcgta acctgtcggg tacagatctt
    16441 ctgctgaacg caccagacga acacgctgac gaggcacgtg gattattttt ttgtctaaat
    16501 atacatcaat cgtatcttca cctatatctt cataaactaa cgcttctttt tggtattcat
    16561 tgacaaatat ccgatcacct tttgcaaaga gctgacgttc ttttttattc tccttcctat
    16621 ttgtaactgc tgtcttagga agaaattctt ttttctgtgt ccgataggct ttttccgcca
    16681 agtaacgctc tgcttgatgg atcaatttca tcgacatatc cattttatga gcaatccaca
    16741 aagcttggct ttctcctgtt tcttccaacc cgcaactgat atttaggacg aagtgcttcc
    16801 cgatcgaaag ccatagcagc cggcacaaaa tcttcatgat ctccggcgaa tttcttgatt
    16861 tctccataat gggtcgtggc aatgatcaat gccccttgct catacataga ttccatgatt
    16921 gcgatcgcta gtgcagcccc ttcatttggc tctgttccac tgccaatctc atctaaaagt
    16981 accaacgtat tcctttttat ctttttcaaa atagcagcga tgttttgcat atgaccagaa
    17041 aatgtactca acgcattttc taaattttgg tggtcaccga catcgacgaa aatctcatca
    17101 aagacagcaa gctctgtccc ttcttttgcc ggaacttgca taccgaacat cgccatcaaa
    17161 gtcaataaac cgactgtctt caacacgact gttttgcctc cggcatttgc tccggtgata
    17221 atcaagcctc gatagtcttt accgatttca acatccaatg gtaccgcatg ctcaggtaaa
    17281 aatggatgtc tgccttgctt gataatgata tgttccgatt tattgattct aggtgtgctt
    17341 ccattgattt ctcggctaaa ttttgcccga gcaaagatga tatctaacac cgtgatcgtt
    17401 tcgatcaata aatcgatcac ttcttcgttt tctgcgatag caccagtcaa tgccgccagg
    17461 acttgatatt cttcactgat ttcttcagct tttaacaact gatagtgttc attcaattta
    17521 gaaatcacag ttggttcaat aaaaaccgtc gttcttttat ttgattcatc gataatcgtt
    17581 ccatctattt tatttttata gctggctttg ataggaatcg tatagtattc gcctttttga
    17641 acaatcattg cttcttggat catttctttg tttttcggat gacgcaagaa tttcagtaat
    17701 tttgactgga tttctttttc gattagttgt aattgtttgc gtactttacg caaattacgt
    17761 gacgcgtcgt cactgacttt attatttttt attttctggt caatcaactc ttctacattt
    17821 atcaaatcag gaagatgttt gctataagca aacaacaagg gtgtctggta ttgattctta
    17881 tcaaaaaact ttgtgatcat ccgactgctt ctaagaaaat ccgcatattc gatcaaatcg
    17941 gttggctgca gaatcaaccc ttttttgact tgttctgtca gtgcatcgat cctcggcaac
    18001 cccataaatg gaacatgctg actgctttct aagatcaagc gtgcttctct tgtttcttct
    18061 tgacgatcca agacagtttg taggtttgtc gagaccgtca tatcgctgat tcgttttttg
    18121 ctatagtttc caattgctct tgcttggatt tcctttttga tccgatcaaa ttgtaattga
    18181 ctaatcattt cgttgtccat attctctcct tttcttgaag agatacctgt cccaaaataa
    18241 ataagggcct agagataaat aaaaggatag aacacagctt aaaaaagtca tgtcctatcc
    18301 ttattagaat acaaagatat tgttttcctg acaggtaccg tttcataagc acaccaaata
    18361 aactttgttt attggctatt cgacttactg attagatacc agctacactc acaaaacata
    18421 ccccattctt ctaggtctta tttattttcc acttaactat agcaagacac attttggttg
    18481 tcaaacttta gtatgtttta tagatttaca ccattaatga atagacacct accgcgataa
    18541 ttgctccgat cattggacct gcgattggta cccatgaata tgcccaatct gaagttcctt
    18601 tattagctat tggcaatacc gcatgtgcta atcgaggacc aaagtcccga gcagggttga
    18661 ttgcatatcc tgttggccct cctaatgaaa gaccgatggc taagatcaat ccacctacag
    18721 cgaatacgtt cgttccaggt gcaaattcgt tttgtccaaa agctagcaac ccaagaacta
    18781 atacaaatgt accaattaat tctgtgatca catttgctgg ataattccgt atagcaggac
    18841 ctgttgcaaa cgttcctaaa atcgcgcttt catctttcgt tgcattccaa tgtggcaaat
    18901 aagaaagcca aacaagaatg gcccctgcaa aagctcctaa aacttgcgca atgatgaaag
    18961 gaacgacaag attccaacta attgcccctg tcatcgccat agctagagag actgcaggat
    19021 tcaaatgagc tgggcccatg aatccagaaa tatagacagc cattgttaca gcgagtcccc
    19081 aaccaaatgc gatgacaatc cagccagaag cctgtgcttt acttttgttt aaattcacag
    19141 ccgcacacac accatctcca agcaaaacta ggatcagtgt cccaataaac tcccccatga
    19201 tttgagtcat atcactgttc atttatttct ccccctttag ttcccgcaga tctgattctg
    19261 caatcaatgc ttctaaacgt ttagtctgtt gcgccttctc ctcttctgtc cattcaaaat
    19321 atccagcaat tgcatcgatc acaggttgct tgatctcatc aagctgatct cgttcaaaca
    19381 acagatgatt tgtacgacga atgagataat cgcctggggc caaaaccatt tcttcttcta
    19441 aaccataacg taaccgagct gactcagcga gactcaaacc aggataaggt gccatttctt
    19501 ttgctaattc aaagatacgt cgggcattag tcccgtaaaa atcagcgata taagtagcat
    19561 cttcttcctc taaaccggct gctactcctt cttttgctaa ttctgtcact gtttcttcta
    19621 atttagttgg atcgaagttt ccacctgaaa tctgatattt tttagagtcg atttctttcg
    19681 tctctattcc gtattcttct tttaataatt ggcgtatcaa tcgcaaagct ccttctgcca
    19741 tcttacggta atcggtgatt ttcccaccag ataaagtcag caaaccatct ggttctcttt
    19801 ctaaagaact acctctggaa atcgtagaag gtgctttttc atcacggctg ttttccaaat
    19861 ggttcaacac gtcttctact tcagcacgag aaactttgtt ttccttatat tcacttactg
    19921 tatcgacaac tttattgaag ctcttgtctg aaatcgatcc attatctcca ccattgtaat
    19981 cagaaccaga attaccaatc aacagtggac gaagtcctgc ccagcttgct tcgatatcat
    20041 tcaatgtgat atttgcttct ggatagcgat gattgatcac atccaataga taatctacat
    20101 cttcttgtgt gactttcgga tccgtaaaat ctccttggta atccgtatct gtcgtaccaa
    20161 agtaagtctt gttttctctt ggaatagcaa aaaccatccg cccatcttgc tttcctgtat
    20221 cgaagtatgt gggttgcggt acaggcagtt ttttcgcatc tacaactaaa tggatccctt
    20281 tagttggacg cattttagga gagactggac gcgtaaaatt caagtttcgt actttatcta
    20341 cccaaggacc actcgtattg atcactaatt ttgctttgat ttcgatcact tcatctgtca
    20401 gcaggtcacg agctttgacg ccaacgattt gatcgccatc atataaaaag ccaaccgctt
    20461 tcattttact tactagataa gccccatctt cggcagcctt tttgatatta tcaatcacta
    20521 aacgggcatc attgttgcgg aaatctagat agacacccgc gccttttagc ccttcttttt
    20581 tcaaaaacgg ttctctttcc aatacttctt ctggtgtcag cgtataattc tcatatttgg
    20641 ttcctgtcac atttgccagt ttgtcataaa ggtccattgc tactttgacc gagaacatat
    20701 tgaaggttgt tgctccttcg ccctcataga ttggcaaaag cattggatct ggttttggga
    20761 tgtgtggggc aatactttgt acgactgcac gttcaccaac tgtgtcagct actacttcca
    20821 catcaaatgt cttcagataa cgaataccgc catgaacaag tttggtcgag cgagaggaag
    20881 tcccttctgc aaaatcctgc atttcgatca atcctgtttt gattcctgat gctgctgact
    20941 ggactgccac accggcacca gtgattccgc caccgatgat caacagatcc aattcttctt
    21001 gctgcatttt ttgaatgcta tcttgtcttg tcttgtttga aaacatatgg atttcctcct
    21061 tattcaaatg cttgggtagc ttttactgct tttttccaac cgttgtaaag tttttctcgg
    21121 cgctcttcct ccatttgaac ttcaaatagc ttgccagctt cataaaattc gcggatctcg
    21181 tctgtatctt tccaataacc gacagctaat cctgctaaaa atgctgcccc tagcgccgtt
    21241 gtttccaagt tttctgctcg ttggatcggt acattcaaga tatctgtttg gaattgcatc
    21301 agatattcat tgtttgctgc accgccatct actttcaata caggaatctt gattccagtg
    21361 tcatcttgca tcgtatcgat gatatctctc acttgataag caatcgactg taaagtagct
    21421 ttaatgatat cttcttttgt cgtaccacgt gtcaagccaa acatagcccc tcgtgctgcc
    21481 tgatcccaat aaggagcacc tagcccaacg aatgcaggaa caacataaac ctcatcttcg
    21541 cttgtagagc gtttcgctgc gtcttctgaa tcgctagctt tttgcaacat ttgaagtcca
    21601 tctctcagcc attgaatcga agaaccagcc acaaaaatac ttccttctaa agcatagtaa
    21661 acctttccat ttatgccgta accaattgtc gtcaataaat tatttttgga taattgcggt
    21721 ttttcaccgg tattcatgac aataaatgaa cctgtaccat aagtattctt gaccatgcct
    21781 ggttcgaatg ccatttgtcc gaataatgcg gcttgctggt ctcctgccat ccctgaaatt
    21841 ggtgcttcgc ttccataaaa atgataacct tgcgtataac cataaacttc tgagttagaa
    21901 gtgacttttg gtaacatcac tcgaggaata tttagtaaat ctaaaatctc ctgatcccaa
    21961 tctagctgat ggatattgaa caacatcgtc cgactggcat tcgagtaatc agtaacatgt
    22021 gcttgtccat cggtcagttt ccatactaac caagaatcga tcgttccaaa catcaattcg
    22081 cctttttcag ctcgttcttg cgcgccttct acatgatcaa gaatccagcg tatcttcgtt
    22141 gctgagaaat aggcatcgat cactaaccct gttttttcgt ggaaaaattc ttgatagcct
    22201 tcatttttta agttatctgc aattccagct gactgtctgg attgccaaac aactgcatga
    22261 tagattggac gtcctgtatc tttttcccat attactgttg tttcacgctg gttcgttatg
    22321 ccaatcccag caatctgatt tggtttgatc cctgattcga tgaacgctcc ggcaataact
    22381 gactgtacag aattccagat ttcatttgca tcatgttcca cccaactttc ttgcgggaaa
    22441 tattgcgtga actctttttg agaactgccg atgtgtcttc ctttcttgtc aaagataatt
    22501 gctcgtgagc tagttgtacc ttggtcaatc gccatgatat acgttgattc tgtcattgtg
    22561 attccctcca ttatatgcaa acgctttctt taactactct tatcatacta ctgttttaaa
    22621 aaaagaaact gtcaaaattt tattcttttt ttcttttttt gacagttttt tattatgaag
    22681 ctatttttta atcaattctt ttcttttttc agcgtctcta ttcttctttt caatcgaata
    22741 atgtcgtaag gtgacgcaaa ttctgacaga taatattcac actgtttttt gtttctctgg
    22801 caaaatgtga tcaccaaatc atattcttta ccttcttcat aacttccaat ctcaataccg
    22861 ccgattcccc tcagttctct gatcagatat tgttgaaaag caatacgata aatcggcaga
    22921 ctttctaaat cgattccaat cactactggt tttgcaatat aaaaatcgat catcaaaaga
    22981 acattggcat actggatcat caggtaacta agctcactat cttcagctct tttcctcggc
    23041 aattgctgca ccgctgtttc ctgtaatttc gccagtaact gtgttaagga atgcccgagc
    23101 attttctgct gctgttccaa aagccgatca cgctcataga cttcgatctt tccaacaaag
    23161 aaaaaccatt cattgttgat ctgggaaatt tggtagccta ctgccttttc ttctttgatt
    23221 ttcaaacgac gaggacgata atgccataac atcgtttccc gaatgtaagt atctaaaaca
    23281 gcggttggta actttttgct ccgtgtcaaa tcatactgat aaaatatttc ttcgtctact
    23341 atcgaaaaag aaacaaaaaa gctgtaaaac atcattgttt cgtatacatt cagttctgct
    23401 gctgttcgac taagatgcat cacgatgatt ggatcgattg cttgataaag ccggtccgac
    23461 tgatagagta atttcttttc tttcaaagtt gcgtacgtac ttttttcatt caatagtctt
    23521 tttttcgtaa tcccgagcca gcaagcaatc tgtgcttctg ctgatgccgt aaacgttgtt
    23581 tctaatacac ggtccaatcc tagaatgaac ggtctttttt ctggtgtatt ttgcaaaaaa
    23641 agcggtcgct gatcttccgg gatatattgg aatagttcat aataaaaata tcgtatctgt
    23701 agctcttctc cgtataattg accgtttttg atttgtaatc caaattcctc taagagctgg
    23761 ttcaattctt tgatctttcg aaaaacagaa gactcactga tattaaacgc tgtcgctaat
    23821 cgaacgatgg atacttgctg atgttctaat aaatagacga gcatcttaaa cttcaagctt
    23881 tcctgcaata aaaacaatag aattttttcc aaacttacat cttcgtccaa cacaagcagc
    23941 gcctgatact catttcgctg tacttctact tttttttcca tcattcggcc aatcaattca
    24001 atatcttcca aatattgatc aaacgaggct tttgacagtt ggacatgctc tctcaaacca
    24061 ttgatagagg ctgttccccc ttgcagaaca aggtttcgca aaatcaggat ctgtaactgc
    24121 tcttttcttt ctaataatgc ttcaatcttc ataggcagta ttcgccacct ttgaccacac
    24181 catatctggc acacggtatt ctggctggaa aaatagctca tgttcttcta actgttcaaa
    24241 cgtccatcct tcttctactg caattgtcag ttggtcaaga aggtggaaca tctctttttt
    24301 gctttggagc tgtgctccaa gaatcttttt ttcagaatca aatagaaatt tgagtttaac
    24361 tttgctttga tttattgctg taagcttgta gtctacgtat cctgtttcaa ttttttgggg
    24421 ataaaaaatg ctctcttctt ctgtcaaacc tacgcttcct aagaaatagc caaaccactg
    24481 attgccgact ggacgatagg ttccctgatc ctttgtctgt gagccactaa ttgtcttgct
    24541 gacatttcgt gctgtacgga cagcgttatt tactagagaa acatacattt tttcatttgt
    24601 tggacgaaaa gttacttgga tcgcatcgcc aatcgcataa acgtcttttt gagacgtttg
    24661 caagtagtca tctacccaga tcgttccatc atcattcaat gttaattgtt cttcccacat
    24721 ctgattgtcg ggacgagaat gattagccag caaaacaaga tcatagttct cctttctttt
    24781 ttccgtctct acaatagcgg tttcattttc ggtcaaagac acaacttgtt cttctaaaaa
    24841 aagtgaaagc ccttgatttt tcatttcctt tactaacggt tctgtcattt caggatctag
    24901 ataacgaaaa agaattgact ttcggctttc gtaaagatgg atttgttttt ttcttgatgc
    24961 caacccttca gctatttcga ttcctgcttg tcctgcacca ataattgcaa ttttctttgc
    25021 attcatgatc ttttgctgaa gagccgttac gttttgtatc tttttaactg ggtgaataag
    25081 cgttgattct gcagaaatat ttcgaaactt ttgatttgaa ccagtcgcta gaatcaaacg
    25141 atcaaaatcc aatttttttc ctcctgctaa aacgactttt ttttctttca atcctattac
    25201 tgtcttttct gtgtaaacat ggattcgata gcggtcagtc agttcttctt tcgtgatcca
    25261 agaaagttgt ttttcactgg taacctttcc ttttaatatc aaattgaacc cgctgggaat
    25321 ccatcctaac ttcgattgct tttcaatcaa atatatttca cttgttggca tcaattggcg
    25381 aagataaata gccgcatgaa tccctgcaaa cgacccgccg acaattacaa tgttcatctg
    25441 cattcctctt tcttataagg tgttctactg ctaacttctc aatcagaaaa cacagtattc
    25501 ataactcgct ttcaacctta tgatacatag cgatgcagca gaatcaaccc tctccatcat
    25561 accgtgttct aggtctgctt ctcaataatg tactagtgga cctaattgaa gagtatcatt
    25621 tctgaagaga tgtttcaaaa aatgtactct tataaaagaa aaaaactcgc cttttacgta
    25681 cctctgtcca aataggaaac agaaaagaac gaaaagacga gttttagcgt gtgtaatgta
    25741 atagttagaa tagacacatc atttattttt aattctttaa tgcttcaaca actgcttcgc
    25801 ctaattgatg agcagattga ggattttgcc ctgtaagaag ccgcccatca atttgaacca
    25861 cttctgaaaa agctgctcca gactgatagt ttgctccttt ttctttcaaa gcatcttctg
    25921 ctaagaatgg tacttcattc gttgtaccat ttgcttcctc ttcttcatta ctgaacccag
    25981 tcactgtacg tccagctaaa atagattttc cagactcatc tgtaactggc aacaatccta
    26041 caacaccgtg acaaacagct gagaccagcc cttcatttcg atagatttgt gaagcgactt
    26101 tcccaagatc tattgcttct ggaaaatccc acattgttcc gtggccacca gcataataaa
    26161 ccgctgcata atcatttgca tccacatcat caggacgtaa ggtatttccc aaggcccgat
    26221 ttctgaaatc ttcatcttca taataggccc aatcgatttc atccatattc aaaacacttt
    26281 gcggttctaa aggtacatat ccgcctttag gactgacaaa atctgcatcg atcccatttt
    26341 ttactaatac atcataaaaa tgagtcagtt cacttaacca caaacctgta ggacgctcga
    26401 tcttttcgta cttttctaca tttgttaata cgatcaatac tttcattttc ccatcctctt
    26461 tctataaaaa ttgcgttatt gataacgtgc tttcaagaaa ttatataatg ggcttggcaa
    26521 ataaagctct ttatatgctt tttcaaaagg tttcgttaca aattcatcct cacggttttc
    26581 caataatttc tctgcccagt caggatcgac gatcatttgc tgtccaattg ctgcaagttc
    26641 tgcatatttc aatacctgct ctacatcttc gcgattgcgg atcccaccga caccaactaa
    26701 agggatcttt ccttgaatcg tgttatggac cacttcaaga atcgatttat cgttgtattt
    26761 atcagaacga gctattcgat catacatatt taatgaaaca tgcagataat ctagttttga
    26821 ctcacgtaat cgttcaagaa gccagatcgt gtcttcaaaa cggatacctg gtgtttcata
    26881 ttcttctggt gagaaacgat accctacgat gaatggtttt ttagcatatt gttcaaccgc
    26941 tgagaaaacc gttgttaata attcttcaat aaaacgataa cgattttcca agctaccgcc
    27001 ccattgatcc tctcgtctat tcgaatgcgg agagaaaaat tgttggatca aatacgtatt
    27061 tgctccatgc aactcgattc catcaaatcc tgattcaatc gctctacgtg tggctttgcc
    27121 aaatgcttgg attgtttcaa cgatttcttc ttcactcatt gctcgtggtg tttccgcatc
    27181 aggacgtttt gctgcaactg cacttgcaga tacaggctgt tctccagata aagttgctcg
    27241 acttgtcatt cgtccaccgt ggaaaatctg aataatcgct tttgcgcctt ctccttggat
    27301 tgctttcgct agctcactta atcgaggaat catttcatca tgagcgatgc tgagttcacc
    27361 tggccagcct tttccgatat cttggacatt tgcagcacca gtaataacag caccaagacc
    27421 tttagaacgt tgtttataat aagtgatctc atcttcggtg atcactccat tgaaaaagct
    27481 gagttgtgtc gtcataggcg acagcatcaa tcggttcttc aattgaactc ctgaaggtaa
    27541 agtaattgat tgatcaaatt gtgacattgt cattcctccc ataattcgct ctgattaacc
    27601 tatcgagaat catctatcat taaaccactc gattagttgt acatgcaact aatttgctta
    27661 aaaaatttta ttttttttgt aacttatctg ttgcatcatt cattgtctgg gctaattctt
    27721 tacttgcttc atttcccaaa actgctttgt atgactgatg aagttcttcc cagctaccat
    27781 cgacttcctt agcaaattgg cgtccttgtt ctgtaagata aactaatgac aagcgacctt
    27841 cgctaactgt tttgacgtat cctttatatt ctaatttttc aacaaaacga gcaatcgttg
    27901 atggggcgga atagagcatt tgtgcaatat ctttttgtgg tattccatct ttttctttta
    27961 atatcaataa aataaaacca taagttggtg ccagacctgt ccttctaaac gcttcatcag
    28021 ctaatttatt caacatgcga tctagctttt tcacgctaaa gaaaagacat tcatctaacg
    28081 ctttttcaat tggcttttcc atttcatccc ctccattggc gtacatatca tagtatgatt
    28141 tttatttttt gtcaaaaaac acggaataat gtccgcctcc tctgtctaga tttgaaaaaa
    28201 agaagtagcc atcttcaaag aatttcggct tgttttcgac aaaactcctt ctggaacacc
    28261 gtgaatcaga ggttgtgaaa aagtcgttta gttttgagta tgaagaaaaa ttttgtgaaa
    28321 atggctttcc acatttttgc ataaatttgg cttaataccg aaaaactggc ttttgaacac
    28381 cgtttatatc agaggttgtg aaaaagccgt cctgcatcaa gtaataagaa cagccaaaca
    28441 aaaatagttc ctcatatttt gtcgtttttt gagctaatgc cgatggtcag ctgattgtac
    28501 accgtttatt gagaggtcgt gagcctgccg ttgaactccg agtcacaagg agcaatttca
    28561 aaaaacagct tctcatgttt tatgactatg gtgacttgtg atcgaggagt tggcaggaga
    28621 atactgttta ttcgtaaaga aggggctgtg acaagacttt tgtcacagtc ccttatttcc
    28681 ttttattata ggatttcgcc atttgattgg atgacttgtc tgtaccaatc aaatgatttt
    28741 ttctttaacc gtttctttgt gccattccct tcgtcatctt gatcaacata gatatatcca
    28801 taacgttttg acatttgtga agtagaagcg ctgacgatat caatgcagcc ccacgacgta
    28861 taaccaaata aatctactcc atctttgata gcttccttca tctgctcaat atgctgcttc
    28921 aaatagtcaa tgcggtaatc gtcttgaacg gttccatctt cttctacttt gtctatagct
    28981 ccgagtccgt tttcagctat gaacagtggt ttttgatacc tatcatataa ttgattcaaa
    29041 gcaattctca aacccttagg atcaatctgc cagccccatt cacttgatgg aagatacgga
    29101 tttttaattc ctccagttat taagtttcct tggacccctt cccaattttc tggatgagca
    29161 ctgactgcac tagacatgta gtagctaaaa gaaacaaaat caacagggta ggctgctaag
    29221 atttcttcat cacctacttc tatttttaag ttgacacctt tttcttcaaa cattcgttgc
    29281 gtatacgctg gataatatcc tcttgcttga acatcactaa agaacaaaga agttctttgc
    29341 tgctcaattg ttttttgaat atcatccgga tcacaagaat acggaacaag aagctgatag
    29401 ctgatcatac aaccgacttg tgaacctttg ataatttcat gacaagcttt tgtagctaaa
    29461 gcacttgcta taaactgatg gtgtgcacct tggtatttcg cttgttccaa atggggatgg
    29521 ttttcttcaa ttactcctac gctaacaaaa gggtgatgtt ttacacagtt gatctcattg
    29581 aaagtcatcc aatattttac tttattttta tagcgtttga atactgtttc agcgaaacga
    29641 acgtaaaact gaataagctt tcgattaggc catccaccat attctgtaac caaatataac
    29701 ggcatttcat aatgggaaag agaaacgact ggctcaatcc catattttag acattcgtca
    29761 aacacttgat cataaaaaaa taatcccgct tcattgggtg tttcttcatc accatgagga
    29821 aaaattcttg tccatgcgat agacatccga tagcatttaa aacccatttc agcaaataaa
    29881 cggatgtctt ctttaaagtg atgatagaaa tcatttccgt gtcttttagg ataataatac
    29941 ttgctgttgg gatcctgcgc cgcttttact tgctgatgcg tcatcatatg ccattgatct
    30001 gtccattttt ctttaggtaa atcgctgtcg aatgtataca catcagacac agataatcct
    30061 ttttcatcta catcccatgc accttcacat tgattggccg ctgtcgctcc gccccataaa
    30121 aatccttctg gaaatttttt catatcgatc cccctaaact tgctagtttt cttgtttgat
    30181 tatttttctt atgactagta atgatatttt tgaatctact actatttgta acaataacca
    30241 ttgtggtcgg atcatatccc tctttgatta attcttgcaa atctacttcc atcaataaat
    30301 cgcctttacg tacttttttt ccatcagata ctgcactttg aaagtatttg ccatttaaat
    30361 tcactgtatc aataccgata tggatcagta tctctgctcc ttcttcactg actaccccaa
    30421 ttgcatgttt cgttggaaaa actgttgaaa cttccccgtt tacagaactg tagatttttc
    30481 cgtccgttgg aatgatcgca aacccttctc ctaaagatcc actagcaaat gttggatcgt
    30541 ttacttcaga taaatcaatt atttcacctg ctgcaggagt acttaacact atatcctttg
    30601 aggattcttc ttcgatttca tccttgtagc cgaaaagaaa agttagcata caggaactga
    30661 tcatcgctaa gaaataacta gcaactagcc aaacaaatcc ttcaccaata taaatcggga
    30721 aagacaaaat acttcttgta gcaactgcca aagcaccggc tcctgcggca ccagctactc
    30781 caccaaatat agctccacat atacaagcaa tgtaaaatgg tcgtttatat ttaagagtca
    30841 caccataaac agcaggttca gtgattccaa ataatgctgt gataaatgca gataaagata
    30901 aactttttac tttcttattt ttcgttttca aaaatacccc aagtactgct cctgcttgcg
    30961 cagctacggc agtcatacat gctggaccta gcgtatcacg accgtagaaa gaaagattgt
    31021 tggtcattac tggtacaagt ccccaatgta acccaaaaat cactaacact tgccaaaatc
    31081 cgccgataac agcaccagat aaaacagggc taaagttata aacagctaaa tatccagctg
    31141 caatcagctg acttgcataa tctgcaattg gacctacaac taataaaata attgggacca
    31201 tcacgatgat acaaatgaat ggtgtcaata aatttcgaat actttcgtgc aagcttttgc
    31261 gaatcttagg ctcaatataa gaaagaaccc atacagctaa aattgctgga ataaccgtac
    31321 ttgtatacct tgctagaatc acgggcatcc ctagaaatcg caaggttatg ctatcactat
    31381 atgcactgac aatcgtagga taaacgagag ctgctgcaat aaccatagcg atgaaacgat
    31441 ctgtattgaa cttttttgca gcagtatatg ccaaaaatat aggcaagaaa tagaaaaatg
    31501 catcagctgc tgcattcaaa atgatataag ttcctgattc agtagttaat aaattcaaac
    31561 tcgtacataa ggcgagtact cctttgatca aaccacttcc tgccaagacg cctaaagtcg
    31621 gggcaaaaac agcggcgatc atgtctataa aagagtttaa aatgcctttt ttctcagttc
    31681 ctctctttcc actagatgca tcatcttcta gtttcatttg tttggataat acgtcataca
    31741 cttcatttac tgcatttcct actacgactt gaaacattcc tccagcttcg actacagtga
    31801 tcacaccttc tagttgctct accttctcct tatctgcctt cttattatct tttagggtaa
    31861 agcgtaacct tgttgcacaa tggtaaacat tgctgacatt ttcttcgcct ccgacaaatc
    31921 ggacaatatc tttcgctaat tgtgaatagt ccatttttac ttcccccttg ttaattaatg
    31981 ccgtagcaat ccttcttaca agataagtat agttcttaga aaacggtttc tccattcaat
    32041 tagtacccct tctaatagcg aacgaaactg tacaatagtg tgtatggttt acttagccga
    32101 gcaaatcact cactgcttga aagaaaacaa gtatatcgtt tctcttatag tcaaaggaga
    32161 caaaaaaaac acgcttgatc ttcctttgct gatgtataag tggtatctca agctgatact
    32221 ctgtgatttt tgtttctact tggcttttga taagacagca caacaaagtc tcggcattta
    32281 acggaagcat aactaggtca gaactaggag agattagttg ctgaatggaa tctatctttt
    32341 tatggatttt tatttgattt tcttctttgt agtaatgatc aagcagtttt gttttctttt
    32401 tttcttcaac tatggtcagc ttttcacgca aaatctgata aatctcccaa gattcaaact
    32461 catctagaac actaggtaga taaatcaaat catcccactc aaaaataagc tcttgctcag
    32521 tagtcaaaat tgcatcatat tccgttattt tgtttgattg atttttgaaa agatacactg
    32581 gttctatccg gcattctttg atccacatgt tcatactttg ttgaatctgt ttttttagtg
    32641 tattcaaaat actaattggc tggttactaa caatcagtat ctccccttgt tttcgaaaag
    32701 gattcaacgc ttcggcaaga tacaaaacaa aataagcgct ttcagctaaa ttgggacgta
    32761 attgaaaatg tattgggaag aaacgccgaa ttaaataaat ttctaaagga taacgggaga
    32821 tgatcttttg tacaaaatga ttcatggtat tatgtccggc atccatcctc gttttcattt
    32881 gtttgatatg ggttagcaaa aattctgttt catggaacaa taacgaaaca ggaagatcta
    32941 aaaaacgaat aatcgtttgc tcaaatgctc ttagattctt ctctatttct ttgttttttt
    33001 catcattaat catcaaataa tttaactcta atagtcgatt ttcaattgct tgtttttctt
    33061 gattggaaaa agaatagggt agttctgatg ataacttttt tgtgagggtc tctaccatcg
    33121 gaataaataa tgtatacctg tttgtttttt ctaaaagttc ttctcttcga agaatggtca
    33181 aaacgagata ccgagaaaat cgtatgaagt cctctcctga aacaatataa ttatatgata
    33241 taagaacctc ttttaatgtc ttttggacag tcggcatgat attggaaaaa attgtactga
    33301 tttgttgagg aaaccttgcg tgttggatca ccgtttctgt tccaacaaga gataagaaaa
    33361 ttctttttga attttcttca gcaatgattt ttagtccttt tgtcgacgaa acttccagtt
    33421 ctaatttagg attgcgctcg atccaacgca aaatcgtttt tatttgtaat gatacggctg
    33481 tttttgaaag aaagaaaatg tctgctatct tttgcatact gacataccct ttttgaaaca
    33541 ataagtacgt cacaatagtt gaagctcgga aatccatgct ggaatagatt ttatgtttcg
    33601 tcacctcttg tatgatttgc tgtttagttt cagattcaat tgattctaaa aaataacctg
    33661 aacgtttatt tgactggata atgattttct tctcgtagat agcaagcata tttttgattt
    33721 cagtttggat cgttttcttg ttggtaccta aagcttcagc taattcttcg cttgttatcc
    33781 aatgattctt atttcttaat actacaagaa tttggagttg tcgtttattt aagtccattt
    33841 gcttacacct cctctttgtt ataaacatac aggaaaaacc tctcaaatga caatagtcac
    33901 ttgcagaggt ttaaaaatca tttgaagaaa gatctatatt caaggttttt accattagac
    33961 tggataattt gtttataata ttcaaaacta tcttttttca atctcctaaa gcttccgctc
    34021 ccatcatttt gtctatccac ataaataaag ccatatcgct tcctcatttc ccctgttcct
    34081 gccgatacga tatcgattgg tccccaccat aagtaaccaa tgatttggca accatctttt
    34141 attgcctcag caatttcctg aagatgtatt tttagatact cacaacgttt tggatcgtgg
    34201 attcgattca attcatctgg tgcttcatct agtccaatgc cattttcaac tatgaataat
    34261 ggtaattcat aacggtctgt caattcattc atgacataac gaaaccctag aggatcaatt
    34321 ggccaactcc aaggttcggg agatttttcc ttcaaaaagg gattatcgaa accatttgct
    34381 ccgccagtat ctacgcgcat ttctgcttct tttttataga cagcgctacg ataataacta
    34441 aatgcaatat aatctgccgt gttagcttgc aagcactgtt cgtctccatc taacattact
    34501 ggtgcacagt cttcttcctt ccatgtcctg taaatataag ctggataatg ccctcggcac
    34561 atgacatcgg agaaaaacca tgaattgcgt ttaaattgca gtgaaccaaa tacattctct
    34621 ggatcacagt catatgcata agtcgctaat gcagaaaagc tacacataat acccatctta
    34681 gcatttggat cgattttctt taataattga gtagcacggg cgttcgctag aaacatatga
    34741 tgacaggctt gataaatcac tttttttgtt agtgtagcaa tgggttcaag tttatctcta
    34801 gcagggaatg taaccaatcc agcagctgca taaggcatac gaaaagcatt attgatctca
    34861 ttaaaggtca tccaatattt cactttccct tggtagcgtt caaagacggt ctgcgcataa
    34921 aattcaaaaa agtcgattaa ttttcgattt tgccaccccc catactctcc taccaatgct
    34981 aagggggttt cataatgact aagagtaatt actggctcga tatcatatcg atttaatgta
    35041 ttgattagtc tgtcataaaa tacgagacca gcctcatttg gcttctgttc gtctcctctt
    35101 ggaaatattc gtgcccatga gatgcttgtt cgaaaagctt ttaaacctaa ctccttcagt
    35161 aaacgaatat cctcttcaaa tcgatgataa aaatcaatcg cttcatgaga aagataatgg
    35221 agtgattcgt ctaaagcaat ctcccaaatc tttttttctt cattccactg aatactagga
    35281 tgctggttca tgatcccgat aagcgtatct gtcacattag gtaatttgcc gtcttccaac
    35341 catgcacctt cacattgatt agcagctact gctccgcccc ataaaaaatt ttctggaaat
    35401 atttcctgat ccataactat ctctcctcgc tatcaacaat ttcttacttg aagtatagct
    35461 cgattcatca aaacaagata ttctaactat ttcggcagaa gagaagaaaa aaattgaatg
    35521 ctactcttca tttttaatat ttatatataa caaatacata agacgatccc cgaaagaaaa
    35581 cactcgttaa ccatctaagc aaacgattta ttttacttcc aacacaaatg cttcataagg
    35641 tcgtaaatca actttgccgt ctggtaactg tttcagagga taatttgaca caataaccct
    35701 taaagcttca cgtggaatag tgaacgtctg cttttcactg gacatattac aaataacgag
    35761 ccattgctgg tcttcatata tgcgagaata ggcaaagaca tgctcaggcg tcttttccac
    35821 taattcatag tctccccaga cgatgatgtc atgttttcta cgcaaatcga tcaatttctt
    35881 gtacatataa aaaacagaat cttgatctgc taacgcttct tctgcattga tttctttata
    35941 atttggattg actcgaagcc atggaattcc agtagtaaac ccagcatgtt cacgggcgtt
    36001 ccattgcata ggcgtcctag cattgtctct tcctttcgcc ttgattgatt ccaagatttc
    36061 ctcttttgta aatccagaag aaatcctttc gttatacata ttgattgtct caatatcttt
    36121 tgcttccgcg atatcatcga ttgggcaatt cgtcatgcct aactcttctc cttgatagat
    36181 ataaggggtg cctttcatca tatggagcaa gatagcaaac atcttggcac tttcttttcg
    36241 atacacatcg tcatttcccc aacgagaaac aattcgcgga agatcgtgat tattccaaaa
    36301 taaactattc catccttcat cacctaaaga tgtttgccat ttggataata cttttttcag
    36361 ttcagagata ctcaagggtt tcaaatccca tttgtctttg ccttcttgct gatctaatcc
    36421 tatgtgttcg aattggaaga ccatggataa ttcatttctt ttaggattgg aataaagttt
    36481 agcgatttcg gtagttgctc cccatgtttc gccaacagtc atcagatttt tgtcaccaaa
    36541 agttgcttga ttcatttcct gaagatactc atgcagttta ggtccattac ttgtgatttc
    36601 tttatccggt tctttgccaa tcatatcgat cacgtccata cgaaagcccc caataccttt
    36661 atcgatccag aaattcatca tttgataaat ctcttggcga acctgtttat ttttccaatt
    36721 caaatcaggt tgacgcttgc tatacaagtg cagaaaatat tgccccgttt tttcatccaa
    36781 ctgccaagca gaaccactga atattgagcg caatctatta ggctcttttc cattgaccgg
    36841 atcacgccaa atataatagt cccgataagg attttcttta tttttacggg cctcaatgaa
    36901 ccacggatgt tcatcagaag tatgattcac caccagatcc atgatgatac ggatatttct
    36961 tttctttgct tcttcaatca gttcttccat ttcttccatc gtaccgaatt ctgccatgat
    37021 tgcttgataa ttactgatat catacccatt gtcatcattc ggagactcgt atactggaga
    37081 aagccaaatt gcatcaatac ctaaatacgc cagataatcc aacctggaaa taatcccccg
    37141 caaatctcca ataccatctt gatttgaatc ttgaaaactt cttggataga tttgataaac
    37201 aactgaatgg tgccaccatt tttgttccat gattttcctc ctataaatgg atgtttctat
    37261 tttttgctta atcatttatt ttagtctctc tcactaaaaa ttgtaacgct ttcaaatagc
    37321 tattgcacct tgttatcggt aacagaataa aacaaaaaaa cagctgagga aattccctca
    37381 actgtttttt gaaaggattg atttatgcag ggaagaatgc tgatttcaca acttctttat
    37441 aaacggtgtt cactcagctg tcccttggta ttagctcaaa aaacggcaaa atataagaaa
    37501 ctattttttc gtttttttct ctaatactca ggccagaacg ctgatttcac aacctcttta
    37561 tttcacattc aatatccaca tgaatcccac aaatacaaag aacagaccat acatgattgg
    37621 agatacttct tttcctcttt tagctgctaa catggtaatt ggatagaaga taaaacctaa
    37681 tgcaatccca tctgaaatac tataagtcaa aggcatgccg atcaagatca agaatgaagg
    37741 gatcgcaatc tctaagtttc cccattcgat ttgtcgcaaa gattgtgcca tcaatactcc
    37801 taccataatc aatgctggtg cagttacttg tgaagtaaca actgatagta atggagaaaa
    37861 gaacaagcct aacaagaaga aaatccctgt agtaatagct gtgatacctg aacgtcctcc
    37921 aacggcgata ccagctgatg attctacaaa tgcaccaaca ggagaagtac caaataagga
    37981 acccgcaagc atagctgttg aatcagaagc taaagctttc cctacacgag gcatcttgtt
    38041 gtctttcatg aaacctgctt ggtttgctaa tccaactaaa gttcctgctg tatcaaagaa
    38101 agtcacaagc aagaatgtca gcactacgac ccacatttga agagaattga tgtcccctac
    38161 atgttttaat gcaactaaaa atgttggttt caaacttggt gctgcggcaa caatttcaga
    38221 aggcattgga atcaaacctg tgaccagacc taaaatcgtt gtagctgcca tcccgatgaa
    38281 gatgccacct ggtacacgcc gaacaagcaa gatagcagtt acgactaatc caaaaatcgt
    38341 caaccaagta gaaccaacgt tgagtgatcc taatcctact aatgtagatt cattcgaaac
    38401 gatgatccca ccttcgctca gaccaaggaa agcgataaat agaccgatac cgccagaaat
    38461 cgcatatttc aaatctgctg ggatcgcatc aatgatcatt tcccgcagct tgaagattgt
    38521 gatcaaaata aaaatcaaag aagcaacgaa tacgccggct aatgctgttt cccatggaat
    38581 ccccattcct aaacaaacag aataagcaaa aaaagcattg atcccaagag caggtgccgt
    38641 tccaattgga taacgggcta atacccccat taaaacacag ccgatcgcac tggctaatgc
    38701 agtcgcagta aacactgctc cttcatccat tccagaagca cctaatacag ttggatttac
    38761 aaataagata taagccattg agataaatgt tgtaaatcct gccagtacct cacgttttac
    38821 cgtggtgttc aatttttcta tttcgaagta ggaaagtatt ttttccttca tcttccatcc
    38881 ccctaaatcc gaacattata aataaagtcc gttaaaattt cgatttatta ataaaacaac
    38941 gtgttttatc atatactaaa acaaaacata agtaaacaaa aaattcggat tttaagcaaa
    39001 atccgaacgt tccatctgat taaatccctt tatctcaaag ttcatgaaca ttcactttgg
    39061 attacaagaa tttctttatt gtgttaataa agtttcttca ttctagtctc aaaaatcaat
    39121 agtgtgattt gattttacca tttcgtttct gttcttcttt gatgaattct tttgtatcga
    39181 caggattcag attatttagt ccgccaaaac ctgtatcgac catttttccc cagccttttg
    39241 tcttcttttt tcctgatttg agattctttg ctcgaagctt ctcactatgt tttgctcgcc
    39301 gatacttcaa ataaccttct aatcttccca ttagatccac tgtttttcct cctttttatt
    39361 caatcagacg actcagcttt tcctataaca aaataattgc cttcagggtc agaaaaattg
    39421 aaagtcaaga tgccattgtt atctgtgatt tctcctggtt cttccatgtt ctcgtagata
    39481 gtttcaaatt catccgtaaa aaatactaaa gaaggcggtg gtcctaatac ctctggtgaa
    39541 aacttctgca caaatacttt tgggaatacg cctaattcta attcttttga gatgctgagt
    39601 acaatactct taaattcttc tggcaattcg attgtttcag aaatttctgc accaattttt
    39661 tctacaaaga atcggcaaat catctctacg tcattgacat aaagcatcac acgtgttttt
    39721 tgtatcatca tttgaccctt ctttctattt gattttcttt cctgtctata aactgattcc
    39781 attatatcac gaaagcgcaa acaccttgtg tttgatacta ttcaagaaaa ataaagcaat
    39841 agtttctagg attgctttcc tccacaaact ttttttacaa aaaaaaaacg gcaatgaatg
    39901 ccgctcttct tatttcgatt cttttataat tctgcttctg attcatcttt ggttagtgtg
    39961 tgtctaacac tagttacgcc aaatgtttga tcgatttttt ccttcaacgc ttcgattgcc
    40021 tgtgcgccac gtgccgcttg ttcagcattg atggattcaa tgaccagcac cgctgcttgt
    40081 gtttcttccg tcagcatcgt tctttgggct tcacgccagt tgagacagcg acagatcgct
    40141 ccttgttcat catagtagat gatttcttcc ggaagtgccg gagcatcgga atctgcacct
    40201 aaaggcagga atgattcgcc accttgggct tttcctaaat gcagatttcc tgcgatcttt
    40261 tcgatatctt ctccgccgca aggtacgcca aaagacaagg aaatactatt atagatatct
    40321 acaagcggat tgattgggaa aaattcccgt tcttgatttg cacgtttcag taaagcttca
    40381 atagaagaac gtgcaccttt tttagtcttg aatgtggtaa atgcctgacg ccactgagca
    40441 atgacttcgt tttggctgaa tgtttcttct gtaaggtaat ttcttgcttc ttttgctgcc
    40501 tcattcaata attgatgata cgtttcttca ctttcttttg ttacgtggtt atcgatacca
    40561 tttacggata agatttcgat ggttgcttca ggaaatactt cccagaaatt ggtatccact
    40621 attacttttt tcatctgttc acacctcttg attagaataa ttgcttgatg aatagatagt
    40681 ctgtttgggg atcatctccc atataaaaaa catgttcgct gtatttgata aatccttttt
    40741 tacgataaaa agcgagcgca acatcgttgt gttcccaaac accgagccaa atggctgttt
    40801 tttgcttttc ttttgctttt tcaatagctg tatctagtaa aaatgtaccg ataccttgat
    40861 gtttaaattt ttttcgtaca tagattcgct cgatttctaa aaaatgatca gccatttcct
    40921 cagattgact cgttcctgta tttagtttca ggtaacccgc aggctcttga tcgatactca
    40981 ctaggaaaaa atcggtttct ttatctttta gctcttctgt aagtttttct agtgcataag
    41041 cttgtgtcaa gtaatcagct aaatgctccg gtgaattatc ttttccaaat gtatctgtaa
    41101 atgtttcgat actaatttgt tgtagtaagt ctacatctga aggcgtcatt tttcgaattt
    41161 cgtatttatt cacggcttct tcctttcaat agatcctttt attgcctttt ttgacaaaat
    41221 tccaatcttc gctaacattc ttgcatactt tatttaaaag atattccaat tgttttgctt
    41281 cttcgtcaga aagaccattt aatgcgactg cattggagta gttgttttct cgaatgatga
    41341 aaggatagat ttctgctcct tttttagtcg gatagagttt cttgattttt ttattttctt
    41401 tatcttccaa acgttcgatc atcccattgg attctaattt cttgattgct cgggcagtcg
    41461 ttgtacgatc tactttgatc atttctgcta gtttttcttg gatgattcca ggattttcgc
    41521 aaatacgtac taaataaaga tactgtcctc ttgttaaatc gacttctttg aattcgatat
    41581 tgctaataga atctagcgct cgggcaatca ttccgatgtc tcgaaggatt tcagccattt
    41641 catcccacct ttattttgtt tgtaagacta gttcacctct ttttgttgca aatgcaacta
    41701 ttttcaataa aaaaatcaag ttctttttta ataactagta aagtatattc ttttcttctt
    41761 ttacgaaaaa gctcaatcac aatagatctg gtttttgttt tctaaaataa gtaactgttt
    41821 cttcgttgtt cgcttttctg atagctttgg caactcttct atactaaaaa atcccatgtt
    41881 ggaagtttca ttattctcaa taaacttggc ctcttcaccg tggatcgcac aagcaaaaac
    41941 aagtttataa tactgaaaaa gctgtggaat atctttttgt ttattcgtat caaaaactgc
    42001 tcgcaactga gtagtttcta ctgttaatcc tgtttcttca tagacttctt tacgcacatt
    42061 ttcttcagga gacaatccta tttcagcaaa tccaccagga agtgaccatt ctttcgtccg
    42121 tttatcttcg attagtagga ccttgccatc ttttttgata taggcacgaa catctacttt
    42181 tggtgttgga tgtccttctc caatcgttgt gagcgctttt atttcttcga aagtttcttt
    42241 tcctatttca ctgatcagtt ccaatgaaat cgtccggatc tcctcaaaac gctctttatc
    42301 aaatacatct tttccataat atagaccagc ttgagaaagt gcgagtagtc ttttgtattg
    42361 agccaacttc ttgttcattc tcttcccttc ttttttagca aatgtatgac tatgtaatca
    42421 agtatagaag actggtttaa ttacggcaaa taaatataat gaaatctgca ataaataaaa
    42481 gaatgttaaa aaactcgaga acaaaagaca ttcgacttct ctggggctgt gaataaatgg
    42541 cgttcaaaag cttgcatctg cggtaataag ccccaccaaa cgaaaaatat gacgagctat
    42601 tcttgtctgg ctgttcttat taattgaggc aggacagctt tttcacaacc tccccactaa
    42661 acggtgttct cctaccaact cctcggtcac aagtcacaat attcatcaaa catgagaagc
    42721 tgtttttttg aaattgctcc ttgtgactcg gagctgaacg acaggctcac gacctctcgg
    42781 taaacagcgt tcaaaagcca atttttcgat attaagccat ttctttgtta cttattgtca
    42841 ttgatttctt tttctaattt atccaatagt ggagtgtaat ccacttgagt atagtctgtt
    42901 acatcaaatt catatagtgt tcctaactgg aatttgtcat attttcggtc ggcgatgatc
    42961 tttttgaatg cttctttcgt aatatgattg gtccctttcg ttcgatcttc caatacgtca
    43021 ccatagtgat aatgatccat gatatagctc aaatgacgat ctgattcacg atcacgtttg
    43081 taccgacgtt gccataacac ttcaaagtct gctgttaggc gaatcgtgat cacctgatat
    43141 ccgtatctat ctgctaattc ttggagtcgt gctttttgct tatcactaaa aggatactcg
    43201 gacacaatca ctttctttcc tgcttccata tacaaatcaa gtgctttgta atagaaagga
    43261 taaactttcg tcctttcttg ttccgcccgt tcttccaaat tgttaaaccc gatggattct
    43321 gcatagtatt ccttgatttc atcgagcgtg atggcataca tatctggaaa tcgttcccgt
    43381 agtttattca taagatatgt ttttcctgta ccaggactac cagccagaag aatgaaatac
    43441 tttttcatct catttatttc ctaagttaat tggactttcc aagttcataa acagtccaat
    43501 cttatgcttg acaatttctt ttactgcttc atttgctgga atcacattat ggcgaagaga
    43561 cttgttgatt tctgtttcac taaaggtttc tttagctact ttcgtccagt tgactaaaag
    43621 ctccgttcca acattgaatt tgcggatccc gttattgatc aattcaggat aatcttcttc
    43681 tttcacacct gttcctccat ggataactag ggggacatca actactgcat cgatttcttt
    43741 cagcaaggga atgtttactt ctgtttttga tttgaattgt ccatgattcg taccgatagc
    43801 aatagctaat gcatccacgc ctgtagcttc tacaaatttc actgcgtctt ccggtcttgt
    43861 atataccttg tcattttcag cgacatggat tccttcttct gtaccgccga ttgttccaag
    43921 ttctccttct acggaaacac cgtgcgcatg ggcatattct actactgctt ttgtcttcag
    43981 gatattttct ttgaaaggca gatgggaccc atcatacatg acagaagtat atccgctgtc
    44041 gattgcttct ttgatatcat caaagtctcg ggcgtggtct aagtgcaaga ctgcatcaac
    44101 catttcttct tctgcaatag ctgtcattac attgactaac acatgcatgc cgatatattt
    44161 agcggtatcg acacttgttt gaataatgat cggcgaaccc atttcttttg ctgcgcctag
    44221 catagcagga agcatttcta ggttatgcgt attaaaagct ccaactgtaa aattcaactc
    44281 ttctgctgtt tttgttactt ctttcaatgt tgtatacatc ttttattgcc accttatctt
    44341 tttatttgtt tcctttgatc gttacttgtt ttgcaatttg tcgcatctca tccattttgc
    44401 ccatgtagta atcgatccct tgatcatcac cagcacgagc agcatctgca cgcttctggt
    44461 tatagaggct catcagtttt ttatacccat ctccgatcgt ctgtttattt tccaaactct
    44521 tttctaaata ttggatggct tgatcgttca catacaattc agaaaattta agtccaactt
    44581 gttcataaag tttcgctaat ttatgcttat cttgggtagt ggaaatctct tgtttcaatt
    44641 gtgtaatctc tgattgaatc ttttcggtcg cttctggcga tagggctttt tcttcttttg
    44701 ccagaacttt tttcggttct ttttcttttt tcttaaaaaa tccaaacatc attttcccta
    44761 ctttcaatta aaatgctttc aaaactgggc ttaagatcca ggcatataag agtgctgcgg
    44821 ctactgtatc tacatcggtt gctgtagcat tgttgaatcc catcgaatta aagatcgtta
    44881 ctagcaaagc gggcaataat gtaataaaga aaccatgagc aataccgcca atgatcgctc
    44941 ctcggcgtcc gccaactgcg ttgccaaata tccctgctgt ccctccggca aagaaattgg
    45001 ttagcatacc aggtaaaatc atcgctaagc cgaacatggg caaagtaaac atggcaataa
    45061 tcgtaccaat cgttgtcgta atgaaaccta aaatgacagc atttgggcta taaggaaaga
    45121 aaactggaca atctaaggca gggatcgcat caggaactaa tttcatagct atccctcgaa
    45181 atgccggcac gatttcacct aacagtagac gtacaccagc tagcaataca tacacaccca
    45241 ctacgaattg gatggcttgt aaaaaggcaa acatgatata gttttgatct cctgataatt
    45301 cagaagcgaa tggttcgcct gcaaataaaa cagtgatgat atatagcggg accatcacga
    45361 ccatgacaga caaataagta tcttgcagaa attcgaaatg tgtcggcaac ttgatatctt
    45421 cgatcgactt tttattttcg cctttctcac caaaaagctt agcaacaccg gcttcaaata
    45481 agtacccgat cgtacaaaaa tgacctaaag caatatcatt tgatccagtg attttacgaa
    45541 tgatcggttg cgctactgcc ggcatagcaa tggcgaaaca agctccgata aaaccaccga
    45601 ccaagatcaa tacgatccct cgcagaccgg caaaatagcc aaagactgtc gtcatcgttg
    45661 ccatccataa aattgcttga ccagttaaaa aaatgtactt ccattttgtg aaacgagcca
    45721 aaataatatt gaatacaaaa atggctaaga acgtcagtgc aatatctcta cctaaaccca
    45781 aatcattcat tgcttgacca ttgatagatt caatagaagg gatgatccct tgcatatgaa
    45841 atccttcagt aaagattttc ccgaaataag ttaaacttcc aacgatgatg ctcgaaccgg
    45901 cactcaacac ttgaaatcct aggagtgtct ttaacgtgcc agagatgact tgtcctgctg
    45961 atttcttctg cagtagtaga cccaacatag caatcaatgc aatggtgatc gatgcctgag
    46021 tcaaaatatt ttcgatgata aaattgataa cgcccat
//
LOCUS       DACCYH010000012        44845 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.012, whole genome shotgun sequence.
ACCESSION   DACCYH010000012 DACCYH010000000
VERSION     DACCYH010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.012"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            212..604
                     /locus_tag="IVY85_000636"
     CDS             212..604
                     /locus_tag="IVY85_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8043672.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="IVY85_000637"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="IVY85_000637"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP8043673.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="IVY85_000638"
     CDS             3165..4775
                     /locus_tag="IVY85_000638"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP8043674.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="IVY85_000639"
     CDS             5067..5936
                     /locus_tag="IVY85_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP8043675.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="IVY85_000640"
     CDS             6101..7372
                     /locus_tag="IVY85_000640"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8043676.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /locus_tag="IVY85_000641"
     CDS             7369..8664
                     /locus_tag="IVY85_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP8043677.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="IVY85_000642"
     CDS             8763..9026
                     /locus_tag="IVY85_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP8043678.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="IVY85_000643"
     CDS             9471..9920
                     /locus_tag="IVY85_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8043679.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="IVY85_000644"
     CDS             9977..11395
                     /locus_tag="IVY85_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8043680.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="IVY85_000645"
     CDS             11395..12255
                     /locus_tag="IVY85_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP8043681.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="IVY85_000646"
     CDS             12429..13202
                     /locus_tag="IVY85_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8043682.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="IVY85_000647"
     CDS             13349..13714
                     /locus_tag="IVY85_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP8043683.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="IVY85_000648"
     CDS             complement(13862..15070)
                     /locus_tag="IVY85_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8043684.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="IVY85_000649"
     CDS             complement(15089..15367)
                     /locus_tag="IVY85_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043685.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="IVY85_000650"
     CDS             15560..16216
                     /locus_tag="IVY85_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8043686.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="IVY85_000651"
     CDS             complement(16658..17455)
                     /locus_tag="IVY85_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP8043687.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="IVY85_000652"
     CDS             complement(17452..18636)
                     /locus_tag="IVY85_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8043688.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="IVY85_000653"
     CDS             complement(18661..19908)
                     /locus_tag="IVY85_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP8043689.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="IVY85_000654"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="IVY85_000654"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP8043690.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="IVY85_000655"
     CDS             21888..22202
                     /locus_tag="IVY85_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP8043691.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="IVY85_000656"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="IVY85_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP8043692.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="IVY85_000657"
     CDS             22887..23531
                     /locus_tag="IVY85_000657"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP8043693.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="IVY85_000658"
     CDS             23546..23875
                     /locus_tag="IVY85_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043694.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="IVY85_000659"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="IVY85_000659"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP8043695.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="IVY85_000660"
     CDS             24863..25687
                     /locus_tag="IVY85_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP8043696.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="IVY85_000661"
     CDS             25680..26027
                     /locus_tag="IVY85_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP8043697.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="IVY85_000662"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="IVY85_000662"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP8043698.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="IVY85_000663"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="IVY85_000663"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP8043699.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IVY85_000664"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IVY85_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP8043700.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IVY85_000665"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IVY85_000665"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP8043701.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="IVY85_000666"
     CDS             31684..32451
                     /locus_tag="IVY85_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8043702.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="IVY85_000667"
     CDS             32441..34483
                     /locus_tag="IVY85_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8043703.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="IVY85_000668"
     CDS             34764..34913
                     /locus_tag="IVY85_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP8043704.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="IVY85_000669"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="IVY85_000669"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP8043705.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="IVY85_000670"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="IVY85_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP8043706.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="IVY85_000671"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="IVY85_000671"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP8043707.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="IVY85_000672"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="IVY85_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP8043708.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="IVY85_000673"
     CDS             39330..40412
                     /locus_tag="IVY85_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP8043709.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="IVY85_000674"
     CDS             40412..41215
                     /locus_tag="IVY85_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8043710.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="IVY85_000675"
     CDS             41285..42025
                     /locus_tag="IVY85_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8043711.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="IVY85_000676"
     CDS             42036..43040
                     /locus_tag="IVY85_000676"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP8043712.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44782)
                     /locus_tag="IVY85_000677"
     CDS             complement(43085..44782)
                     /locus_tag="IVY85_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043713.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14413 a         7826 c         9340 g        13266 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataa
//
LOCUS       DACCYH010000013        43624 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.013, whole genome shotgun sequence.
ACCESSION   DACCYH010000013 DACCYH010000000
VERSION     DACCYH010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.013"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            74..1714
                     /locus_tag="IVY85_000678"
     CDS             74..1714
                     /locus_tag="IVY85_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP8043714.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            1868..3190
                     /locus_tag="IVY85_000679"
     CDS             1868..3190
                     /locus_tag="IVY85_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP8043715.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3252..3683)
                     /locus_tag="IVY85_000680"
     CDS             complement(3252..3683)
                     /locus_tag="IVY85_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8043716.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3747..4583)
                     /locus_tag="IVY85_000681"
     CDS             complement(3747..4583)
                     /locus_tag="IVY85_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP8043717.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(5325..5951)
                     /locus_tag="IVY85_000682"
     CDS             complement(5325..5951)
                     /locus_tag="IVY85_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043718.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(6246..6581)
                     /locus_tag="IVY85_000683"
     CDS             complement(6246..6581)
                     /locus_tag="IVY85_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8043719.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(6568..6852)
                     /locus_tag="IVY85_000684"
     CDS             complement(6568..6852)
                     /locus_tag="IVY85_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP8043720.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(6908..8431)
                     /locus_tag="IVY85_000685"
     CDS             complement(6908..8431)
                     /locus_tag="IVY85_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8043721.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(8424..9599)
                     /locus_tag="IVY85_000686"
     CDS             complement(8424..9599)
                     /locus_tag="IVY85_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8043722.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(9603..9788)
                     /locus_tag="IVY85_000687"
     CDS             complement(9603..9788)
                     /locus_tag="IVY85_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043723.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(9754..11448)
                     /locus_tag="IVY85_000688"
     CDS             complement(9754..11448)
                     /locus_tag="IVY85_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8043724.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(11445..11918)
                     /locus_tag="IVY85_000689"
     CDS             complement(11445..11918)
                     /locus_tag="IVY85_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP8043725.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(11987..12142)
                     /locus_tag="IVY85_000690"
     CDS             complement(11987..12142)
                     /locus_tag="IVY85_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAP8043726.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(12276..12656)
                     /locus_tag="IVY85_000691"
     CDS             complement(12276..12656)
                     /locus_tag="IVY85_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8043727.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(12660..12875)
                     /locus_tag="IVY85_000692"
     CDS             complement(12660..12875)
                     /locus_tag="IVY85_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043728.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(12878..13285)
                     /locus_tag="IVY85_000693"
     CDS             complement(12878..13285)
                     /locus_tag="IVY85_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAP8043729.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(13551..15026)
                     /locus_tag="IVY85_000694"
     CDS             complement(13551..15026)
                     /locus_tag="IVY85_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAP8043730.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(15016..15864)
                     /locus_tag="IVY85_000695"
     CDS             complement(15016..15864)
                     /locus_tag="IVY85_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP8043731.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(15901..16263)
                     /locus_tag="IVY85_000696"
     CDS             complement(15901..16263)
                     /locus_tag="IVY85_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043732.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(16563..17187)
                     /locus_tag="IVY85_000697"
                     /pseudo
     CDS             complement(16563..17187)
                     /locus_tag="IVY85_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(17184..17495)
                     /locus_tag="IVY85_000698"
     CDS             complement(17184..17495)
                     /locus_tag="IVY85_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043733.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(17539..17832)
                     /locus_tag="IVY85_000699"
     CDS             complement(17539..17832)
                     /locus_tag="IVY85_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043734.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            18026..18673
                     /locus_tag="IVY85_000700"
     CDS             18026..18673
                     /locus_tag="IVY85_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8043735.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            18734..19879
                     /locus_tag="IVY85_000701"
     CDS             18734..19879
                     /locus_tag="IVY85_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8043736.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(19960..20265)
                     /locus_tag="IVY85_000702"
     CDS             complement(19960..20265)
                     /locus_tag="IVY85_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP8043737.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(20290..20721)
                     /gene="ruvX"
                     /locus_tag="IVY85_000703"
     CDS             complement(20290..20721)
                     /gene="ruvX"
                     /locus_tag="IVY85_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP8043738.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(20718..20984)
                     /locus_tag="IVY85_000704"
     CDS             complement(20718..20984)
                     /locus_tag="IVY85_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP8043739.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(21239..21964)
                     /locus_tag="IVY85_000705"
     CDS             complement(21239..21964)
                     /locus_tag="IVY85_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP8043740.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(22325..22395)
                     /locus_tag="IVY85_000706"
     tRNA            complement(22325..22395)
                     /locus_tag="IVY85_000706"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22361..22363),aa:Gly,seq:ccc)
     gene            22512..23264
                     /locus_tag="IVY85_000707"
     CDS             22512..23264
                     /locus_tag="IVY85_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP8043741.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            23280..23759
                     /locus_tag="IVY85_000708"
     CDS             23280..23759
                     /locus_tag="IVY85_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP8043742.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(24010..25590)
                     /locus_tag="IVY85_000709"
     CDS             complement(24010..25590)
                     /locus_tag="IVY85_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP8043743.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(25583..26326)
                     /locus_tag="IVY85_000710"
     CDS             complement(25583..26326)
                     /locus_tag="IVY85_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8043744.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(26502..27278)
                     /locus_tag="IVY85_000711"
     CDS             complement(26502..27278)
                     /locus_tag="IVY85_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8043745.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(27526..28362)
                     /locus_tag="IVY85_000712"
     CDS             complement(27526..28362)
                     /locus_tag="IVY85_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP8043746.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(28373..29302)
                     /locus_tag="IVY85_000713"
     CDS             complement(28373..29302)
                     /locus_tag="IVY85_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP8043747.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            29494..29847
                     /locus_tag="IVY85_000714"
     CDS             29494..29847
                     /locus_tag="IVY85_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8043748.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(29921..30577)
                     /locus_tag="IVY85_000715"
     CDS             complement(29921..30577)
                     /locus_tag="IVY85_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8043749.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            30803..32737
                     /locus_tag="IVY85_000716"
     CDS             30803..32737
                     /locus_tag="IVY85_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8043750.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(32823..34046)
                     /locus_tag="IVY85_000717"
     CDS             complement(32823..34046)
                     /locus_tag="IVY85_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP8043751.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(34040..35938)
                     /locus_tag="IVY85_000718"
     CDS             complement(34040..35938)
                     /locus_tag="IVY85_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP8043752.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(35932..37050)
                     /locus_tag="IVY85_000719"
     CDS             complement(35932..37050)
                     /locus_tag="IVY85_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8043753.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(37133..37966)
                     /locus_tag="IVY85_000720"
     CDS             complement(37133..37966)
                     /locus_tag="IVY85_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8043754.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(37959..38876)
                     /locus_tag="IVY85_000721"
     CDS             complement(37959..38876)
                     /locus_tag="IVY85_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8043755.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(39103..40227)
                     /locus_tag="IVY85_000722"
     CDS             complement(39103..40227)
                     /locus_tag="IVY85_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8043756.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            40410..41318
                     /locus_tag="IVY85_000723"
     CDS             40410..41318
                     /locus_tag="IVY85_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8043757.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            41459..43072
                     /locus_tag="IVY85_000724"
     CDS             41459..43072
                     /locus_tag="IVY85_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP8043758.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT        13229 a         8692 c         7290 g        14413 t
ORIGIN      
        1 acctgctctt tttgactata tcctcatctg actcctatac ttcgtttgta ttaaaactta
       61 ggaggaatca ttcatgctta cagggctaaa cttattaaat aatccttttc taaataaagg
      121 aacagctttt acaaaagaag aacgggcaaa atacggaatc actggtatgc ttccaagtac
      181 tgtccaaaca ttggaacaac aatctgtcca agcttacggt caatacttaa gcaaacagac
      241 cgatttggaa aaacgtatct tcttaatgaa tctattcaat acgaaccgga cattgtttta
      301 taaattgatg ggcgaacatt tagttgaatt catgcctgtc gtttatgatc ctgtcgttgc
      361 ggattcaatc gaacaataca acgaaatttt cttgaaacca caagatgctg catttctttc
      421 aatcgacgaa ccagaaaata tcaaagcaag tttgaaaaac gcagcagatg gacgcgacat
      481 tcgtttgatc gttgtgacag atgcagaagg aatcttaggt atgggtgact ggggtgtgaa
      541 cggtgttgac atcgctatcg gtaaattgat ggtctacact gctgcggctg gtatcaatcc
      601 tgcacaagtc ttgcctgtat caatcgacgc tggtactaat aacgaagaat tattgaataa
      661 tccattatac ttagggaatc gtcatgctcg tgtagaaggc gaaacttact acgaattcat
      721 tgatcaattc gttcaatcag ctactgaatt attcccagaa ttgttattac attgggaaga
      781 ttttggacga ggaaatgctg caacgatctt agaaaaatac gaagataaaa tcactacttt
      841 caatgatgat atccaaggca caggaatcgt tgtactagct ggtgtattag gtggattgaa
      901 tatatcaggt gagcaattga aagatcaaac gatcttgaca tttggtgctg gtactgctgg
      961 tgtaggtatc gcaaacatcc ttttagatga aatgatccgt caaggtgtac ctgaagaaga
     1021 agcacgccaa catttctatc aagtagataa acagggttta ttgtttgaag atacaaaagg
     1081 attgactcct ggtcaaatcc cattcgctcg taaacgctca gaattcacta atgctgatga
     1141 attgacaaac ttagaagcag ttgtaaaagc aatccatcca acgatcatga tcggtacttc
     1201 tacccaacct ggagcattca ctgaagaaat cgtgaaagaa atggcggctc ataccccacg
     1261 tccaatcatc atgcctttgt ctaatccaac aaaattggca gaagcaaaag caaaagattt
     1321 gattgaatgg acggacggaa aagctttagt tggaacaggt atccctgctg ctgatgtgga
     1381 atataacggt gtaacttacc aaatcggtca agcaaataat gcgttgatgt acccaggttt
     1441 agggcttggt ttgattgctt caacagctac acgagtaaat gctgaaatca tttctcaagc
     1501 tagtcgagca ttaggcggaa tcgttgatgt aacaaaacca ggagcagcta tcttgcctcc
     1561 agtagcaaaa atcactgaat tctctcaaac gattgctgaa actgtcgcaa aatcagttgt
     1621 tgcacaaaaa ttgaatcgtg aagaaatcac ggatatcaaa gaagctgttg aatctgctaa
     1681 atgggttcct gaatataaat ctttagaaga ttaagattgt tatagtaaaa gaacatacag
     1741 aaactttgta tgacgtctaa aaagaaaaaa gcgactgcgg caaaacagca gccagccaac
     1801 tactgctctt ttgccgcttc tctttggctt ttttcatacg atacagaata agaaaggatc
     1861 tgtaaaaatg gcacaaaaaa caacaaccgc aactgcagtt gaggaaaaga aaaaagaagg
     1921 gttttggtca acaaaaatta ccggcgtaag tttgcccctt tatttgatca tggtccttgt
     1981 cctgatcatt gcaatggctt taggaaaatt accgacgaat atgattggcc caatcgctgt
     2041 tttggtcatt ttcggtaatt tatttcattt tatcggaaca aaaatcccta tcgtcaaaag
     2101 ttatttaggt ggtggctccg tttttgcgat ctttgcctct gccgcgattg ctacatttgg
     2161 gatcttacct gagtatgtag taaaatccac agaaaacttt gtcagcaata tgggcttttt
     2221 agacttttat attgctgcct tgattactgg tagcatcctt ggtatgaacc gtaatctatt
     2281 gatgaaagct tctgttcgtt ttattcccgt tgcacttatt tcaatggtcg tctccttttt
     2341 tgccgtagga ctcgtaggta tgcttatcgg aaatggtttt gcaaattccg ttttatatat
     2401 ctcattgccc gcaatggctg gtggtgttgg agccggagct gtacctttat caacgattta
     2461 tgcaaatgtt ttaggtacgg acgcttcttc tatcatttcc cgcctgattc ctgcgacagc
     2521 aatgacaaat gtcctagcta tcattggtgc agcgttagtt gctcgtgcgg gacaatcaat
     2581 gccaaaattc aacggtaatg gtaaattaat ggaaaaaggc gaccttggcg atggaaaacc
     2641 tagaaatgta aaaccttcca ttccacaatt aggtgtcggt ttgatggtat cattgacttt
     2701 cttcatctta ggacaaatct gtaactattt tgttcctaaa gtccatgctt acgcattcat
     2761 gatcattatc gttgttatct gtaaaatcgc aaacctatta ccagaatatt acgaagatgc
     2821 agctatcatg ttcaacaact tgattgtcaa aaacttgaca gcagctgttc ttgccggtat
     2881 cggtattgcg ttattgaatc taaatgtatt ggcttctgct ttgacatggc aatttgttgt
     2941 cttatgtttg acaagtgtta tcgcgatttc agtagtttca ggatttgtcg gtcgtttgtt
     3001 tggtttatat ccaatcgaat caactattac tgctggctta tgcaacaact cgatgggtgg
     3061 tacaggaaat gtagctgtct tatctgcagc taatcggatg gaacttattg cttttgctca
     3121 aatgggcaat cggcttggtg gtgccatcgt tttgatcatc tcaggtttct tgatgcaatt
     3181 actttcttag tcaaaaaaaa tacctgatca tcaagtatcg gcttgatgac caggtatttt
     3241 ttgataaaaa tttacaggat tttcaaaaat cttttttgca ttttatcgga atgataaccg
     3301 atgcgttcat aaaatcggtg tgcttctact ctgtattctg cagaattaag tcttattgct
     3361 gatattcctc gtcttttcgc ttcttctacg ccagtcatca aggcctttcc gattcctctt
     3421 ttttcagcat gtttagacac agcaagtgcc ataatattga acatcggatc gctgaaaatc
     3481 gattcatagg actccgcttg tacatagcca gctgcttttt tagaagtagg atcttcatag
     3541 accaagaaaa aatttttttc tggctccttc aacagtttct tcatttgtct caccgtaacg
     3601 gttaatggta catcatatcc taattgttcc gcattgattt ctcgtagtgc ttctgcatct
     3661 cctaaacgca tttcccgcaa catccaaatc accagccaat ttttattttt ttaaatgaat
     3721 ttttttatca cagtatactc cttttttcat ttagaacaat atgtttcagt gattttgact
     3781 tgcgttagtc tagattctcc taatgtttgt aagatgacga aagcattgta aagattgatc
     3841 aaaagaacca cgaggacaat tacttgaaaa ataaaggtca ctttttttgg agacaaaacc
     3901 ctgctaaatc ttgctcccca caaacctcca ataacagcag cggggataac ataaaacaac
     3961 atagataaat caaagcgcat aaagctactt gttaaagcaa tcgttaccaa ttttgccagt
     4021 tgagaaaaga aaatcgtaga tagagaatat agtgttgctt cttttattgg caatgcaaac
     4081 atcagcatca acaaagaaac attgactggt ccaccaccaa tacctaaaaa actagctaaa
     4141 aagcccagaa cccccccaca tataaggtac caagtccatg acgtcaaatg aaattttggc
     4201 tgatgatgct tggtgtagaa gaacgcaaaa accaaggtca ataccgtcaa aaaaatttgg
     4261 atcagctgca catgcttctc gttttcaaat aactgtaaaa aaacttcgaa tacaatgttg
     4321 ccgactactc ctccaacaac agctccacta gatacccaac cgacaatctg ccaattcagt
     4381 gatttcccgc ttgatacttg tcgcacagtc gaaacaatgg acatcgtgaa taccgcaact
     4441 gttgagtaaa aagaaatccc tgcaacagaa tgggcgccga taagatcaag aatcggtttg
     4501 atcaacacgc ctccacccat cccggaaata gcgccaactg tgttcgccag aacgatcacc
     4561 acaaaataaa tcagaccaat catcttgtat ttcctttctc ttgtttatat cttttagcga
     4621 ttgttactct tttcttttat tatacagtaa aagaaacaaa cgaggcttcg aaagcttttc
     4681 tactttttaa ggaaatttag tgctaaacga aaagaggttg tgaaaaagct gtcctgcatc
     4741 aagtaataag aacagccaaa caaaaatagc tcctcataat tttcgtttgg ttgaacttat
     4801 taccgcagat gcaagctttt gaacaccgtt taagaaagag gtcgtgagcc tgccgttcag
     4861 ctccgagtca caaggaacaa tttcaaaaaa cagcttctca tgtttgttga atattgtgac
     4921 ttgtgaccga ggagttggca ggagaacacc gtttaaggaa gagactgtga agtcaacgtt
     4981 ttgacctgat tattagagaa acaaatggca aaatagttct tcatatttcg ccgtttttcg
     5041 agcgaatgcc gaggggcagc tgattgaaca tcgtttattt ggaaaaaggg attgtgacaa
     5101 gacgtttgtc acaatccctt tttttatatc ttattaattc attcttttgt tctattcttc
     5161 ttcttccgcc atgaatgaga aacccatatc aaagaactat aaaggttatc aaaacagcgt
     5221 ttatgactaa atgaattaga gttatctatc actatttttc aagcgattta aaccaaatgg
     5281 catacaatat ggcatacaaa aacttcaata agccgtgggg gtttttaaat ttcttcttgt
     5341 tctttaaaat caatgagtgg agctacataa tttgaatcca ctttttcaaa cgtcactttt
     5401 tgcatcgacg aatgttttct ttcccaagat aacgtaaata gatttgactc tggacttata
     5461 aagtctactt caccttctag tattactttc ttatgcaaat attcctctaa taaatataga
     5521 cttgctaaag catacaacac attttccaaa tttgcttcct tataatttga agaacgacca
     5581 tgttttactc tgttatatat tttccaccaa tctggtgtat cttcaggcca cccatctcca
     5641 aaaggtttta gtgaagtcaa tgttaaagaa ttcataaccc taacaccatt atccaaacac
     5701 attaattcgc tagatttaat aacagcttta aaatcagtta tgcttttatt gctactgcta
     5761 tcaaatccgc aaagttcttt gaaaataatc tcacactccg aaccaatcgc tagtagtagt
     5821 ttttgatatt caatagagaa tgttttgaaa ttattctcat cgagcatcac aaaatctgtg
     5881 gtattaataa actcattttc taaatgtaga taatacttcc aataaaaatt tgtaaactct
     5941 gctttgtcca ttaaatcacc tcttttgaat tataacaaaa aaaacacgtc cacaacagac
     6001 gtgttctttg atttctacac aatcagcttg ccaaaaccat ccaatcagtc ctagcatgcc
     6061 tgtattaaaa ttgaacgttc atcatctatc atctttctta gtccagcatg tttgcaacca
     6121 cacctacaat tttcagtcca acaatagatt tcgactagct aagtcttgca aaattcaggc
     6181 agctaaccct tcatttacta ccttaattat actaaatacc cacaacgttt acaagtgtaa
     6241 tcattctaag aaatctgttt agctatgatc gttgtataat ctttaaacgc cgttccttca
     6301 ttgattgaaa taattttata actcttgttt ttccaagcta ctttcatatc gtttgttagt
     6361 tcataatctt gatcgtatcg aataataaac gtcaacgttc cttcaagaat ggtacctaca
     6421 ctagcaagca cgtcttttag catttgtgaa cgcacattag cccaacattc aaatacagtt
     6481 tcttcactat ccaccatctg cccatcttca tcttttaccc gttttgtctt tttaaacttg
     6541 actctctcag tcaattcatt tacattctta atgattggca taataagcac ctctcatttg
     6601 ttgtactaat gcttgtaccg ttactggtat ccgttcgctg ctggcttctt gacggttcaa
     6661 ataccaatgt tgagtaagta atgacaccgc caaatcaaat tgctgataat tatccatatc
     6721 tttatccgtt gcgctactat caatagcact ttttatatat tcgcctgctg tatcaatcaa
     6781 ttgttgaata aagtcatcat caatagtgtg atcgatccgc atactttttt ttatgctatc
     6841 caaaattacc attttatctc tcctaatgaa aaaaaggcag tgggataaac cgccgcccaa
     6901 tatttttcta tacttttggt gttacaactg gcgtaaactc aatatacact gaagcatttt
     6961 gatcaatttt cttataatcg ttgcgaacga tcactgcaag tccttgtgag tagtaatcga
     7021 atttttccca ttgagtcgtt acttgatttc tacgagcaac aaagatagat tgtgcaatat
     7081 cccccataat catagggaat gttccagctt taggattggc aaacaatgta tctgcaatca
     7141 atactactgg cataccgaat aatgatttac cactaggtgc tgtcacatcg ggttgtaaaa
     7201 tgtaacgtcc atctgaatct ttcaatgtat ctaggtggta atatccgctt tggtttagaa
     7261 ttaccatttt atttaatgct gggtctaatg ccacattgta tagttgtttc aaatcgtcca
     7321 acgtagcagc cgttttctta gtgaatgttt ttaataaatc catgatatgc ttattgtcag
     7381 tgttttctac caatttcgct aactgatctt tgacctcttg tacaatattc actgctgaat
     7441 cttccacaac ctcatttgat aaggcaatct taccagcacg agtttctact ttataatcaa
     7501 ctgaagtaaa catttcagcg tcaatatcac caatttcagc aagttcagct tttgttgcta
     7561 acactgcttg ttggttagtt gctactggat atttaccttg tccatttgat actgttttta
     7621 ctgttgcata ttgagctaag ttataatttg aacgtttcaa atcaaagact tcaccgatta
     7681 cttcttcggg aacaactgcc gctgcattta cagtagtgac tccatcacgc acttcgcctt
     7741 gtgaacggat atattcttca tacccacgca attctgtttt ttcgttatcg attaaagttt
     7801 tttccatgtt attactctcc ttttgtgctt tagaagtgga ttctaagcta ttgtttgttt
     7861 gttttgcttg gttgttactc ataaacgatt cgtaagaacg cttatttact tggacatttg
     7921 atgaatcata agcgggaacg gtcacgacac taatttcatt aagtgctttg attttatcta
     7981 ttgatcgttc aattgtgcca tcttcttttt tgtcgaatga atctaatcca agcacgaacc
     8041 caaaggacat agaatcaaca accccttttg agatattctc atacacatca tttgaatagg
     8101 tcgtgtcggt tagctctgct tcaaaatgta atcctgtatc atcgacattc aatttcaacg
     8161 tacctgcttt gacactagct agtggctttg aataatcgtg tccatataat aagaacacat
     8221 ttgataaatc cacctctttt aatgcttcgg gtgtaatcac ttctacaaag ccgcctaaat
     8281 cttttgacgg ttgcccgaat ttcaacgcat agcctgaaat agttttgccg tctgttggtt
     8341 gtttttcatt ttctttctct actttgggag aatcggctgt tagctcagct tcttctgtca
     8401 gccgtttttc cttttcttct tcattattca actggtgtaa ctccttcctg ttcttttaat
     8461 tgatatttct ccaacgtatc taaatacgta aagttcaaac ttgctagtaa acgatcgcca
     8521 ccatccatgg ggggtagccc cattttcgac cgcccctcat taatcgtgag aagtgaccct
     8581 ttgacctgat ccaatacatt ttttactttt gtttcaggat cagtctctag taactgatcg
     8641 gaattaaagc ggatattggt ttcaagtttc gtatctaatt cactcacaaa cacattaaag
     8701 taatgaatca gcgtactttg gatatactgg agattacttt gaacggtgct agaatgttca
     8761 ttttcaacgc ctaaacgctc aatcggtaca ccaaacgcct tggcaatttg ttttgtgttc
     8821 caatcattgg aattgataag cttcaaaaca tcggtgttta tttctaatgt tttgtagtcc
     8881 attgtttcat caagaatgat cgttctaaga gcattatctc cactacttga accgttggct
     8941 tcttcaaact tttcacgaat tgcgttcttt gctgatccgt ctaaatctga cttatgaacc
     9001 ttcaaaatac ctgacccact gacaccacga gtaaagaaat tgtgcaatgt gcggttgcca
     9061 gcttgttgaa ttttcagttc atcacgtaac gcataaagtg gtggtaatcc tgttaaacca
     9121 tcttgggtga aatatttgaa gtgcaaaata tcgctagact tcacacgtct ttttttatca
     9181 ccaatctgat aagacaacgt accattatct aattgagtaa ccgttacact tgagttaggt
     9241 aataagtgaa tttcttctac cttttcaccg ttgcgtttaa tctctgcaaa gctattaccg
     9301 tttagcaaca tattgaccgc caaagcaaac tttaaatgcc aaccatccat ttctgaatta
     9361 ggtttctcgt ttagtaactt caccagttca ttatcagctt gtggcatatt gtttttgacc
     9421 aattgaatcg gacttgaagc aatatcacta gcaataattc gaacggctgc aaacacatca
     9481 ctatttctta atgcacgaac gcttgtaaag ctggtggtgt aatcatccga ttggattgat
     9541 accacatgtt gtaggaaagg atctcccgtt gtattttgtc tcgctttaaa aaatgccatc
     9601 ttttaacctc ccttctgttc tgtatttagc atgaccgcta ccccaataag cactatgccc
     9661 agcgctaaca agccatagta gacatttgtt agtaaggtga tagcaaccaa cacacacacc
     9721 aatccaagaa gtaaaagaac ggtatgaatg taattagaaa gaaaattctt cacttgtata
     9781 aaattcattg tctgctttcg ctccttccgt tgtggtgaag taatccattg caaacacata
     9841 ggcattgatt aatgcagcta ttggatcaat tttgttactg ttctttgatt tattaatctg
     9901 aatgccgttg ttatccgttt ttaaaatagc gttgttcact gcatgggtga gaatcgtatt
     9961 tttattgtga ataacgttgc cttcataaac ttgttctcta aaagttcggg tcggaacgtt
    10021 tagagtaatc gttccttgtc ttacttctaa cattgggtag ttggcttttt cagctttact
    10081 gataagcgaa ttagcgttat acgggtcata acaaatcccc atacattcca aatcattttc
    10141 ttgaatcaaa tcttgaataa actgaaatac ttggtcatag tccacgattc cactttctaa
    10201 ttgagtaatg gaacactcac cagctttttc tagttctcga taaggcaaac cgtcgcgctt
    10261 ttctttgtct tgaagcccat atttggtggc tacaaagcta tgtgaatcac aataaagttc
    10321 gccgttatct agcggtacga tccatgaaac gctagttaaa tcatctgttt tagataaatc
    10381 cactccgata taaacgggct tgcctgtaat atcttgtggt tctacttcgg ttgcctgcca
    10441 gtcttcagca atcatatagc tgtcctcact cgcttgtctc cataaattga agtttttcac
    10501 caatacagaa ttaaggttat tttgtttcaa agcaaggttc acatcatctt gaatggttgg
    10561 aatcattact tttttgattt cttcactttc aaaaatcgga ttggctttga tccatttctc
    10621 ttgatcgtga atttcttcgg gatcgtccaa ttcccatatc gctataaagt acctgtcggc
    10681 ttgttcttcg ccttttagca cacgatctaa caataagtat tcctcgtaca taggaacgtt
    10741 taaatcaagc ccagcggtac taataacaca caataagccg ttcttttgtt gggtcattcc
    10801 tgacttaatg acgttgtacg ttttacgagt ttttgcttcg tgccactcgt caagaattgc
    10861 ggttgttggt gcaaacccat ctagcgtgct ggtttcacta gccagtgcca tagcgaacga
    10921 attagagggc aagtgctgaa tcttagagtt cataattttc agttgtggtc ttaaaaactt
    10981 actagacttg accacgtttc taagtgaatt agacagcata tcatagccca atttagcttg
    11041 tttcaaagcg ttacttacaa acaatacttg ccttgcttct gctggttctt gttccataat
    11101 caacgcattg gcagccatgc cacttgcgat atacgttttc ccattcttac ggctcatact
    11161 ggtaaacgct cgattgaatc gcctgtaatt cccttcttta gtacgccagc cataaagact
    11221 gcctagaatg aatttttgaa atcctaacat cttgattgct ttaccgtccg tagatggtag
    11281 caactcaatg aatttaatcg ctttggtggc ttgttcttca tcgaacacat aagggaagct
    11341 atctgactta gaacgctcca aatcgcttat atgacgctca cacgcttgga taattttctt
    11401 gggtgcaatc agcttacctg ataggacttt ctcaatgtaa tcattcatga attcatcaac
    11461 tctttgaacg ggtcttcggg ttcatcattt ttaaccttgt tcaaagctag ttttgatcgt
    11521 gattctaagg tcatacctag tgaggtggct tgttttttca aatcactcat agcttgtgat
    11581 tgaacccgaa ccgctggatt ggctttcttt actccgtttt cgcatgtgat aaacgtacct
    11641 gattttctta tctcggcttc ggctgttttc attcgagcaa aggctaaaca ataggctacc
    11701 aatgaaccat aatctgcttc actcaatgga taatcttttt ttaatgttgg tacgatacga
    11761 ttccattcac ttctagcgct cgctgccatc cagtcagggg gcgtgatatt tattaatggt
    11821 tgctgcttga acaattcttc tctagcgtcc acacggattg ctttttcttc acttgatata
    11881 ttgccttttg tatcgtgtaa tattttaggg tgtcccatgt tgtcctcctt tcagacaaaa
    11941 gaaaaaggaa cgtaagcaat taagctcaca ttccttcgtt tttcgatcag aatttttctt
    12001 tatctaattt atctatataa atgatttttc cgttctgcat ttgaatggtc acttgaccaa
    12061 aatgaggtaa ctcaatggtg cgctgtttcc ctgttttatc agtaataaca attgtatttt
    12121 tcaataaatc tcctactttc attatatact ttttcacttg tgattacaag tgtaatcgtt
    12181 atttgtaatt acccttatgc cttttcacaa cggttatttg taccaaaatg agtaggcatc
    12241 gattgtcagt aagtttcaca ctatgccccc ttaactcaaa gggtactctt ttctcgtctt
    12301 tgtctttcat tggctgtctt ggaattatgg tgtgcatggc ataaagaacg aaaattagtt
    12361 tcatctaacc gtctattcca atcatctttt agttcaattt tgtgatctac aacatcagct
    12421 ttccttatca agccttcttc taggcaatcc tcacatactg gatgatttaa ccgatacaag
    12481 tatgaagcct ttcgccatgt tctactttga tagaattgaa aatatttacc ttcgctttgc
    12541 tttctttctt cacgcttaat tgccgtttgc tttgctttat gcttcccaca atacttctgc
    12601 ctataatcaa tcaatgtttt acatccagca tgattacatt gttttttaac tgccatagat
    12661 taccaaccaa agcgatctcg ttcgtcagaa tcatcttgtt cccattttga tttgtattca
    12721 atataaggcg aaactatctt caagcacgtt ccgtctttaa aattaacttt agtgatttta
    12781 tcttcctcaa aactaataaa gtctacatct tcttgagtgt ttgaatactc aatgtgtttc
    12841 tcaccatcga agtatctaat actttccata tgcataatta ttctacctcc aataaattag
    12901 ttatcaattc atcatctgtt agtagcaaag ccaagaactt atcatggatg tttaaaaaca
    12961 aattatcctc attcgatgta agctctcgtt gtctcgctcg atcatatagg ttaagcagac
    13021 aattaaattc atccattagc tcacctgtat catcatagtt gccactatct accaatgatc
    13081 ccactactag cgattgacac cacgctactg actcctgcag ccaatcaggt tcttcttcca
    13141 ttatctttaa tacttctgga tcaaatgtgg aataatcttc tgttaccaat cccacactaa
    13201 atgccagcgt gttcattaga aaccgatcca tttcatctgt atattgaaag ttcggtgctt
    13261 tgtttgttga aataatattg agcatttact ttcctccttg attcgtaaca cgtggtaaca
    13321 cctacgtaac aggtacccgt taccactcaa tcccaacggg gacaagggtt ctagcgtttt
    13381 ggtaacaggt aacgggtaaa atccagtaac attattcccc agcatattag ctaactattt
    13441 caaaagtttt aaggaaataa ggtgttaccc cgttaccaaa ttgctgtatc ccttggggca
    13501 gtaagggtaa actagtaacg ggctaggtgt tactgacccg ttactacccg ttactttttg
    13561 tactttccag ctcgattttt attttcatag gcatttgact tattgtaaaa tcctcgtcgt
    13621 ttatttcttt ttggcagttg cttccgttcc caaccaggaa tactgctaat aatcaaggca
    13681 atctttttag tttctgcatc tgatcgacca ttcaataaat cctttgcttg tttatcaaaa
    13741 acagcttcca aaatatcagc ggtgagtacg ctatcaacta aataaaactc ttgttgcata
    13801 gtagctccat tatagaaatg ctgtaaattt ttctcattac atttactgtc aggatagttc
    13861 tgataataag aacgtctcgc ccacgctgga gcttcttccc atttatacgg gatttccatt
    13921 tccaaatatt tagtgattgc ttctttaatt ggattctcca tcttggcatc ctcttgatag
    13981 tcttttgcaa tttctaagac ttctttatct tcatcgggac taaaatagat tcgttgtcct
    14041 ttatcaaaaa gcacctttgc ttcagctaac acttgaagaa aatattcatc ctttgtttta
    14101 aacggattca tctttggctt attcttacaa ggcaaaggat aaaaacggcg gtttcctgta
    14161 tcatctttca aatactgccc atcattcgac gttccaataa atacacattg ccttgcccaa
    14221 gctatgacat tccgaccata aggaggtcta attttatcga tcttggcact gatgaagttt
    14281 tttatctgtt caattttagt tttgttcatt gaagataatt ctccaagttc aataattaca
    14341 ttgccttgaa gctgcatata atcatctttc ttttcaccta gcgaatctaa tgaatcggtg
    14401 aaaaaatcat ccgtgtaaag taatgctgga acggtactct taccaatccc ttgcggtcca
    14461 tccaaaactg gaacgatctc aaactttatt cctggtttat agatacgtgc gactgatccg
    14521 actatccaac gttttgttac ttctcgtgtg taaaggttgt cgtctacccc taaaaggtca
    14581 ataaacagtg tttccagtct aggctgttga tcccatttaa ctttttcgat acgttctttt
    14641 actgggttga aaacattctt tctagccaca tttactactg ctttgtgtat tttgttgtca
    14701 tcaaaaacta cattgtagtt ttcttcaata tatgccgtta aggcatcctc aaaactgtct
    14761 tcccattctc cagcggtacg attgatccaa ggagaatctt ttaataggtg aatgtatccg
    14821 ctaaattcgt tataagcaat tgttccagct aaattgctat cattggttaa aatcaattct
    14881 gcattcacaa cactgttact tttaatctga tcgtctttat tgcatcttaa tcgtgacatc
    14941 caggttttca attcaatgat attgtcttca cgctcgctgg cttcgggaaa gtctaaaata
    15001 ctatctaatt tatgcttatg agccatgagc tttccccctc aaatcttttt taatcatcga
    15061 tcgaataatt tgattcactt ctttatcttt tagcggtggt tcattgtacg catttgctgt
    15121 ataaatcaat tctgctaaag tttgattatc tacaccgtgc catagtaacc aaccgcataa
    15181 cttagttaaa tgatcgtttc tactaccttc gccagcgcct tgaacaatca tatctaattt
    15241 ttttgctgtc cctgtttttg ctacgggtcg gtattgagtc tttatttctt gcggtttttt
    15301 tagcagtagg ttcactagcc aatcgggagc tggtgcaata ttatttagag atacgttttc
    15361 aataggttca tatgctctcc caacacttct tgaaataatc atagttggcg ctattggaac
    15421 tcctttagtg ataatatcaa tcccgcttaa acttttacct ttatcatcac gcccgaagat
    15481 atcagcaaat gcacctgttt cttgttggat aattacacca tcaggaattt taaagaagaa
    15541 atggattcct ttacctggtg tgatttccgt ataagtagaa ggaaatggat catacattga
    15601 agcaagtttt ttataattct caacgccgtt tcgtgtttca gaatgttgat ctaggtcaac
    15661 taccaccatt tgatgatctt caagcattaa cccaatattt ctagttgcat cttccgtcca
    15721 ccattcatta atttgattag aatcgttagt tgcatcattc caaccactag agccttttat
    15781 tggaatttta gttccaggag ataagggata aattgaccat cccgactttt gtaattccgt
    15841 tgctgcttga taattatttg ccaataaacc tctcctcctt tttcaaaata ctggtataaa
    15901 tcattcagac acctccatga gcttcaactg ctcaagttcg tatttcaacc catcccgaac
    15961 aaattcagca ccttttaatt gattctgata ctttacccat gtattaaatg ttttcaccct
    16021 tgcttcttgc atatcagtaa tgtttaacca tgcggttaaa atctgatttt ttagctcgtt
    16081 gatttctcga caaattattt tgtgttctgc atgttttttg agtacataca aaactgttag
    16141 tttttcattg aatgaatttc caaagtaggt tttcccgata ttattttcta gttcagcgat
    16201 cccttgatta atttcctcta attcaaggct atcgagttgg tttaatagtt ttgttttgta
    16261 catctactta tgccctccag ttcttaattg atagtcgtac tcgtcataag cagtgaagag
    16321 atataacgct aatcctgcaa agatagctat ttttgcaatt aatggtaata agccgaaaat
    16381 tccgccgatt aaaatgcttc ctataattaa gtttcgtttc ataatggttc cctccgattt
    16441 tttaatatgg tataatcgga acaaagaata tcgggtttta tcccaattaa gttgccttta
    16501 tcctgtccgc caagactgtt atggataagg ctttttcttt gttctcgatc atgttaatgc
    16561 tcctaagttg tctgcaattc agcagaacga ttataaattt tctcactcac tgaaaactgc
    16621 aacccatatt tttctttaac agtcattaat tctactgtct catctaaaat aggttcacga
    16681 tcaaccaaca tttcaggagt catatctttc ttttttacca ttttggaatg accataaata
    16741 gttgatacag ctttattggc tatcacattc gctttaatgt agtctactgg ttttggttca
    16801 gtaatagcgt ggcttaattt agtcattgct tctttttggt gttctttgtc tagcatgcgg
    16861 aatacttgaa agccttcaag tcctgtggac tggcgtagtt ctttgatgat gtcgaaaatc
    16921 cagtcttgaa atgcctcggc ttctttttta cggcttttga atgccatgcg gtaaatattt
    16981 tttttcgttg atgatgttta catcttgtgt tctgcccaat gaatcgatga ccttactagt
    17041 agtaacccca tctttaggca aagagtgaat ggctctagta acctgtttaa gacctaacgc
    17101 ttccgcaaca tctttcgcca ctgcccacca ttcatcatta atatcgacaa atcttataat
    17161 gtgtccgttc caaatttcag ttttcattca acgtcctcca gttcaagccc tagcaagctc
    17221 gctaacaact caacttgctg atacaaaatt tcttgatact cttcaataga aagtcgttct
    17281 ttaaactctg cttgtactaa atcgttcaag ccacgcaata ctccgcttac cgttggttct
    17341 tgtggtagca atttttgata gtccatttgt tcgtacaaac attcaaacgt ttcttctagc
    17401 gctttgcctt ccaagtttgc taattctgcc gctgtgtctt ctgtaagaat tgttgataat
    17461 actttttcga ttgctttatt ttgtgtcgat tccattatta tttcctccta ttaatccctt
    17521 tgcccccacg gcttgcgttt atttttggta ttcttccata aagcgatcac attcatcttg
    17581 tgaaattctt acgactccat caactataat caactttaaa ccgttaggaa catatttctg
    17641 catcatagta ttgtatgaaa cgttcatata cttagcagct tgtgccttgt tcatatattt
    17701 tggaaactgg tacttttttt ctaataatga aagtaacgtt ccttgagttt gctctacttg
    17761 ttgaacaact cgatcgtcaa taattttaaa agctggatca tcaagcgtga gtttgtcaat
    17821 ttttaattcc atatttatcc cccctcgtta gcatacattt ctatacaaag attttaaaaa
    17881 aatgcaagag gctcttgaat acatttctat acctcttata aactcaatat accatcatac
    17941 cttttcattt gcaagaattt taacatactt ttttgaactt ttatgtttca cacatattta
    18001 aatcgtgata tagtagagaa aatatatgaa tggtggtgaa aatgtggcta gaaaaaaata
    18061 ccccgaagca gtggagacag gacaaagaat tagactggct agaattgaaa agggactctc
    18121 aatggaacaa cttggaggta aattatcccc accggccagt aaaggtgccg ttagtaattg
    18181 ggaaaatggc tataatcttc ccaataacac caggcttaaa caactttcaa aggtattaga
    18241 tgtatccact acctatctat taaacggatc ttatacatta agagatatgc atatgatgcc
    18301 cgaaattaag cgaaatgtgc ttgaaaagca attgaaaaaa actattgaaa aaaataactc
    18361 tataattgat tctaatcttt ctaatgctat caataacatc gaagaagata acctgactgt
    18421 ctcacagaag atttatttaa caaacgcaat aaaatatctt aaaacaggat ctcgaattga
    18481 atttcctgac gggagtaccc caattgctta tctcgcagct cttctaaata cgcttttacg
    18541 atatgactca ttaattaatg attcagaaaa aaatacagat gagaaaaaag agtatcaaga
    18601 attttatgaa aatgatcttc taaaagagtt cacagctctt gttactgagc taaataaaca
    18661 ctattcaaaa taataataaa ctgcctcctc caaccgcccc cacggcttgc tacactatgg
    18721 agggaaaact attatggcaa cattcaagca atatgaaact aaaaaaggta aattttggct
    18781 ctatgaagcc tatcttggaa tgaacaaaat gactggcaaa cctgataagg ttcgtagacg
    18841 aggttacaaa acaaaaaaag aagcccagct tgccttgtca cgcttgcaag tggactacga
    18901 taaaaacggt attaaaaaag cgaacaacga aacgtttaaa gaagtttatg acttgtggct
    18961 agaaacatat aaaaccactg tccgagaagc caccttcatg aaaactgaaa tcaagtttag
    19021 aaagtggatt cttccaaaat atggaaatct tcgtgtgaat gaagtcacgg taaaaaaagc
    19081 ccaacaaatt gtaaatagct gggctgaaac tactgaccaa tacaaggtac ttcattcaac
    19141 ttctaagcgt atttttagct acgctatcaa tcttgggatt attgagacta atccactaga
    19201 caacgtatta atgcctaaaa gaaccaccac taagcataaa gaaaaggtca aagtttacag
    19261 taaagaacaa ctgatcacct tgtttgatta cctaaacagc aaacctgaaa cttatcgaaa
    19321 cgactttgat aaaacgttac tacgctttct cttttatagc ggggttagga tcagtgaagc
    19381 ccttgcattg aattggtcag acattgattt tcaagaaaat acggtcacca tcaataaaac
    19441 actaagccaa tccaaaaatg gctataaaat cagtgatcct aaaactgatt gtagtgctgg
    19501 ggttcttcca ctagatgaaa gaacaatttc tatattaaaa aaatggcaga ttaatcagcg
    19561 gaaatatatg ctgacacttg gtgtaactga tccgactatg attttttgtg gtatctataa
    19621 agaactaatc acacaccacg ctatttatgc aagattgatt actatttgtg aaaaagctat
    19681 ggttccattt ctcggtgtgc atgtagcaag acacactcat gcttcaatgc tcttggacgc
    19741 tggagctact atggtagaag ttcaaactag attacggcat acaaaaatta gtcagacgat
    19801 cgattgttac ggtcacttgg caaaagaaac gaaagaaaag actgttgaaa aattagtaca
    19861 acacttaaat attgactaga tggcatacaa tatggcatac agtaaaaaat aaaaaaagga
    19921 gaaaccctat catatcaagg tttctcctag ctttatagtc tattcttctt cttccgccat
    19981 gaatgtattg aatacttctt caatcatgtc ccattctgcg tctgtttcga tttgttctaa
    20041 ctcgccttct gttccatctt cgttttcgat gtatgcataa gcttgtaatt ctacatcttc
    20101 atcttctgaa acaccagcag gatataaaag aacatagtta cgtccaaatt cttcttgtcc
    20161 gtcaactgtt agtaggattt catataatgt ttcatttcct tggtcatcca ccaatgtgat
    20221 gtgttcgtgt ccttcatggt cgtgggaatg gttatgttct gtcataagat tacctctttt
    20281 cttttattat taaaataatt gcttacctga gccgtctaaa taattttgta agatcatcac
    20341 agctgctaat ttatcgatga ctttttttcg tttggctctt gaagtatttg cctgctctac
    20401 tagcatacgt tctgcctgaa cagtggttaa tcgttcatct tgataatcaa ctggcaagga
    20461 gaatagttct ttgatctttt ctccgtatga catggaagct tctgcccttg gtccgatgga
    20521 attgttcata tttttaggta atccaactac aaaccggtct acttcatatt gtgctactaa
    20581 ttccttcaac cgatcaaagc caaactcttc ttctgcttca ttgatccgaa tgatctctac
    20641 tccttgagct gtccagccaa aggggtcact gatagcaaca cctactgtct tagaaccgac
    20701 atccaagccc attactctca taaattcact ccatgatttg agaggtagtc cttgacaatt
    20761 acttccatga tctcatcgcg ttcgtgacgg cgaatcaaat ttcgagcatc acgataacga
    20821 ggaatgtagg acgggtctcc agaaagcaag tagccgacaa tctgattgat tggattgtag
    20881 cctttttctt ccaaagcccg ataaacaatc gctaatgttt cgctcacttc ttttttgcga
    20941 ctatcgtcaa aaccaaaacg tacggtttca tctgtaaaac ccatgattct acacctcttt
    21001 cccgcaaggt aattccagct taccttctac aaccttattt tactcgaaga aagggaaaac
    21061 tacaaacaaa tattccaaac ttccgaataa attaagaaac actatttgta tgtttttttc
    21121 cttttttctg tagctttatc aaaagtactc ttttcataaa gtatacgcta tatttctttg
    21181 aatgtatgtt tttacacaat tttttcataa tattcataaa aaattcgaat agctgctact
    21241 aattttttgt ttttgttgct tgtcctgtct cataattgat cgtgtagcca tcttgaacat
    21301 tgtagataaa gacattgaat tcaatttttt tgtcttcaat gctttctgct tccaattgca
    21361 ctcccctggc taccagttca ttcccctcaa aatgaggagt gacacggtag cgtacatgat
    21421 gatttgtttc tttgatatat gccgcaatat cattttcgtg agcaagcatg tatggagcat
    21481 tcaacgaacg tgtccctgtc atcaagtttt taatgttgtt attttgacca gtcagctgga
    21541 aaccaattaa atggctgcga ttatataacc attgcccgtc acttaatttc ttttgtttcc
    21601 aacctgtcgg gtcaatatac aaaggctctc tcttttcagt agggaacaaa tcttttccaa
    21661 gcatagcatt ggcttgtcct actctgttca atttatcaat atttgaatag ctttcccatg
    21721 ttcctttgga aaggctaaga tcctcttttg taaattccgg ctgattccca ttgatttcta
    21781 tttcttgatg ttttccatca tattctggca catccgctac ttgagaagct tgtggtgtgg
    21841 aatcaaaaat agttgtaatc tgctgaaaga tatctgaatc tttttgtgtg gtggaaacac
    21901 cgaatgcccc tgcaatcaca gcagcgacga tccccacgat cgccttggtt gtttttttgc
    21961 tcatttcttt actcctttac taacagctcc ctaaatctta acatatgttc ccctataaat
    22021 aaatactaga aagaaataga aacagcaaaa aataaaacct gttttaagat ggcttaaaaa
    22081 tacaaaaaag agacggattc ctcgtctcta atcagtcacc actctatctg taaggtattt
    22141 attattattc aaaaattttc ttcatactca agacaataca ctgatttcac aaccgcttgg
    22201 taaacggtgc gcaaaagctt gcatctgcgg taataagtcc atccaagtta aaaacataag
    22261 aagttgtttt tgcttggctg ttcttattac ttgttgcaga gcagcttttt cacaacctat
    22321 tttaagcggg tgacgggaat cgaacccgcg acatcagctt gggaagctgg gattttacca
    22381 ctaaactaca cccgcaatac aaaaacagta tacgcctctt ttattccttc ctcaagtttt
    22441 tttattcttt acgcttctgg tatgttttgt agtaaaataa aactaatcaa tcaattattg
    22501 gagaagatgc tatgacggga aaagaattag atctgttgct ggacacttgt ttactcgctg
    22561 ggaaaatcat gatggaaagc aatgcggaga tgtatcgggt agaagatacg atgagtcgaa
    22621 ttgctttagc ttctggaaat ttccgtttgg tcagctatgt cactcaaacc ggtctcttta
    22681 ttggtcttga tcgtacctcg accatccgaa tggaacaaat taccaaccgt tcgattaatt
    22741 tagaaaaagt agtaaaagta aatgatcttt cacgtaaata tgttgcttca gagctaacat
    22801 tagaagaact ttactgcgaa ctgcaagcaa tcgaccgaga ccgttctttc tttcctgtct
    22861 ggttgcaaat cgttagcgcg gcagtgatca gtggaacaat tatggtgtta tttggcggaa
    22921 agatgaccga tttacctgtg actttagtca ttggaggatt gggatacgca gcttatctat
    22981 atagtttgaa attttttaga atcaaatttt tatcggaatt tttatcttct ttattgattg
    23041 gcatagccgc tatctacagc gtacgttttg gcttaggaag caatcaggat ctaatcatta
    23101 tcggatgtgt gatgccttta gttcctggag ttcaaattac gaacgctatc cgtgatttgc
    23161 tagctggaca ttatgtctca ggagtttctc gcggaacaga agcgatgatg actgctacga
    23221 tgatcggttt tgcgattgct tttattttcc agctttttta ttaggaaagg aacacaagta
    23281 tggggacgat atttattcag ttttctttta gttttttagc gactgcggca tttgccgtta
    23341 ttacaaatgt acctaggcgt tcattgattt gttgtggtct ttctggtgct tttggctgga
    23401 tgatctactg gatctgtgtt caaataggcg gatctgctgc ttttggttca ttgcttggtt
    23461 ctttaggagt agcattcatc agcgatcttt tctctaaacg tttgaaaatg cccgtaacga
    23521 ttttcaatat tcccgggatg gttcctctag tccctggtgg tttagcttat caagcggttc
    23581 gaaatctcgt tactgaatcc tatcgagaag cggctcatta tgcggtccaa gcaattatga
    23641 ttgctggagc aatcgcttta ggtcttgtat tatcagaagt ctttaaccat aacatccgta
    23701 atttcaaagt aaagcgtgac gcaatcatga tgaaacgaaa aaaacggctt tttaaataaa
    23761 cgaagtgtaa caaagaaaag gttgtgaaag agacgttcag ccttgagcga tgacaaaata
    23821 tctcacaaac attgcgacaa gtaaccgcag gagcaataag aagtaattat gagtattaaa
    23881 aaaagttttg taaaaatagc ttctcacatt tttacataaa attgacttaa tactgagaaa
    23941 ctgacttttg aacaccctct atctgtaaaa aaggggctgc gacatgattt ttgtcacggc
    24001 ccctcttctt tattcaaatt ttttccaaaa cgttcgttga taataaacga tccatagagc
    24061 ggaaagtatt agaactaaac cgatcacagg aatattcact aaccaaaatg gataaaacaa
    24121 agccaacatt ccgtataaga cgataagaat aatactgatg atcaatgaac ccatcatagt
    24181 tgcgactaaa cccaaacttc ctgaacctct gttaaataat tggttaatat tggtccagtt
    24241 cagcagcagc aaccgataat ctctagcgaa ataacgtagt gacaataagt agcagccgaa
    24301 taaagaacca aatagcaagc tagggataaa aaacaaaggc agctgaaaag acagtccgct
    24361 taataatgcg atcccaccag ttaaaaaact ctgcaatatc catccgaatc tgaacttttc
    24421 ttttaaatat tggttcatag agattggcaa agaacggata aacagaaaat tttcttgatc
    24481 caaagaaata agattgctga taaaagaagt ctgattaact gttaagaaag ccaatgcgat
    24541 ccctaccagg aaaacaacac cgattaactt attatctaaa tcagttaatt ttatttcgcc
    24601 tcctagggca aaagcaagaa taaaaatgat tggagttaac aaggaagaag aaagaacttg
    24661 cataatcaaa ttaggttctt ttactaattg agcattgtaa ctaaataaaa gctgatgcac
    24721 attttggttc tttttatggg ttctttttat tttgaactca ctgtttgaat cagtcagttg
    24781 ctctgtaagc tttggtacta tcagctgttt tacagcaagg aaaaatccca gagccgtgat
    24841 agctagtatt ccccaaccca ataaaccttg ttgagaaagt ggctgactgg cagcatcata
    24901 gaatggcaat aatatcgaaa tcggatgccg atctgttaac tcattttcaa tagtccccgt
    24961 ttgatgattc atccacatga ttccagcaaa tgcgatcagc atcgagctgc caagcaagat
    25021 actagaaact gtttttctat gttttttaaa cactgctgtt cgagccaaac caaaaacaat
    25081 caaactagcc agacaaaaaa gaataatcaa aaatagagaa aacaaaagga ttgagataat
    25141 gatagtaagc aaaatcccta gatcagctcg ccatccggtt aaaataaaca tgactagaag
    25201 cggaaaaaca aaaggaatga tggtcaaagc aactaccaaa atctttgctg aaaaaacctg
    25261 ttcttgtcca aaaggcagag gcaaaaatac ttgcaggtca tttccttcaa aaaagatatt
    25321 ataaatgact gatatacttt gagttaaacc taacatacca aaaagtgcca cgtaataagt
    25381 aaagaaacct ggcaatttaa caaagttcat agcaaacata gcaaatccgt aaatcagtaa
    25441 aaaaatcagc cctgaaaaaa gattttgaga caaaagatag atagttaatc gctttccctt
    25501 ttttcctttc tctcttgctt tattcgtgag ctgtggatta acatataaaa gattaactcg
    25561 aattaattct aataattgtc gcttatacat acggtccatc tcctgtcgct tgccttccag
    25621 ccattttcaa ataaatcttt tctagcgttt catcaggtga gccattcaat aattcactaa
    25681 ctgccccctg ataaatcaat tcacctttct tcaaaatagc cagtttatcg cataactgtt
    25741 gtgcggtatc caatacatgt gtagaaaaaa tgactgtttt tccttttgcg gcatgagcct
    25801 tcatcatttc tttcaaatca aatgctgctt gaggatcaag tccttgcaaa ggttcatcca
    25861 atacccaaat atctggatct ggcagcaacg tgccgatcaa aatcgttttt tgacgcattc
    25921 catgtgaaaa acttgcgatt gtttcatttt gatgactata catatcaaaa agcgctgtca
    25981 attctgctaa acgcttttct tttttttgtg tgtctaattc ataggcagct gaaatcaagt
    26041 cccaatattc tccagccgtc aattgtaaaa aaatatctgg ggaatcaggc acataaccga
    26101 ttttttgttt gatactcaat cgattttccg tcaatttcat tccatctact aaaatcgttc
    26161 ccgatgatgg ttcaataata ctaactaaac ttttaatcgt agttgatttt ccggcaccat
    26221 tatgtcctaa aaatccaaaa atttcccctt gtcttatcga gagagacaag tcttttaatg
    26281 cttgtttttt cccgtaactt ttacttacat tgatgaattc aatcatttct gtactccttt
    26341 tgtttgttat tactttttat tgtacccttc gttattggtg gagtaaatgt caaaaagagt
    26401 gcataacaaa aaaggataga aacgactagg ctgctacttg ggttgttctc ttctacagga
    26461 aaacactctt ctaacaggta agatatgagt ctttgttttc tttaaaatga ttcattttct
    26521 atcttttctt gcataaaatt ctttagttta tcaatacttc tctttcccat ctctagttgt
    26581 tctccatttg caaatgttac tatttcgttt actctgctaa tagatgtaat gttttcagta
    26641 ttaatgatat aagcttttaa atctttacat aaatactcat attcgagtga ttttttatat
    26701 tttttaattg tgccgtttac gagaatctct tcatctttca atactaggcc tattttattt
    26761 ttataacctg gcatgctcat tagataaagg atttcatcgt aatggatata gtgatcgcta
    26821 ttaccataac ggacagttgt ccaccttttt ttcgtagcat gttgaacagg atagttttct
    26881 ttttctttca ataagtaact ttggatgctt tttttatttt tttcataagt gaattctttt
    26941 atcaaataac cactaggcgt aatctttcga ttaataacag ttattgcttt attcggatga
    27001 gacgttagat aaacaatttt acacttttca ttcttttctc taagcatttc gcctagttcg
    27061 ataccgctat aataggcgtt caaatcgatg tctaggaaaa aaatatctga ggaatagatt
    27121 tcattatttt taatgaattc aacaaattgt ataacgttca atccctgaac gatctcaaat
    27181 ttccaaggac ttaataaaat tctggattct tccaaaattt ctactagcat tttttcaaaa
    27241 accaaactat catccaaaat aaatatacgc gttaccataa aaagtacctc cactcgatat
    27301 tttactatca aaatcatttc atacaacttt tcaacataca tgttaggaat aaattgtaat
    27361 tctcttcaca atcttttcca acacctttga aaccgatatc aaatactttc aggatgctgc
    27421 ttctaaaata aatatcaaac aaatgaatac atcatgcaaa aaaagccaga caaccgacgt
    27481 ccttgcgtgt cagctgtctg agctatgttt atttattttg ttcttttaaa aagaacgata
    27541 cacatactga tttttcggag cagagacttt ctcagcgttt gtaacaacta cagatgggat
    27601 ttcacgcttg aatggttgat aatagtctga ttggatggtt ccttctattt tataccattc
    27661 atcattttta accgttactc cttccggcat atgcgtcaac aaaccaaaga ctcctgaatc
    27721 tgccacacaa tggataatgc cgaatcggaa caagaagata tctgtattgc tatcgttcgg
    27781 tgtttggtaa acaaatcctt catagctgat ttttttgcca atgaattcgc tagggtaatt
    27841 ataaattagc tccataactt ctaaatagtt ttcatctgtg atcttgatag tttgcttatc
    27901 cccatacttt tctttcaact cattcatttg gtctaagtag tctgatttgt tgaaataaat
    27961 actggtatcc ggctttaagt attgtgtctg catctccgga tcccctactg attctttact
    28021 tactgggaaa ttaaatcctt tggcttctac gatcgtagtg tcgagactaa ctgttggaaa
    28081 caacgtcccc acgattactg gcaaagctag cagtacataa gcgattccac gttgataagg
    28141 ttttgacaac ccatggtcat gatcgtgctc gtgtgccttt tcttctgctt ttccgttttt
    28201 gacccacaaa tacagctgaa ctaaagccaa aagagcagat aaaatcattg aaagaatagc
    28261 taagtagcga taatggacat tgatatattg atcgagtttg ccagaaactt gcaagtacat
    28321 catcaattcg aaatacccag ataaaatcaa aaaccgcagc atattttccc tcctaaataa
    28381 ataatgtgaa tccaataacg ctaagggcaa tcagtccgat cagcgataaa ataaagcgtc
    28441 ctgtaaaata tcgtttcatc atcagtagat ttttgatatc gaccattggg ccaaagacca
    28501 aaaaggcagc tacaggtgct gtgccgaaca gacttaacaa agaagatcct ataaacgcat
    28561 ctgcttctga gcaaagagac atcgtaaagg ccaataacag catgatgata atggctaaaa
    28621 tctttgttga gccgagcgtt agcataattc tagttggtaa ataagtctgc atcgcagctg
    28681 cgattaatcc gccaatgatc aaataacgac ctgtatcaaa aaattcgtcg attgaatgtg
    28741 tcaacacatg cccagtttga tggatcagct gattttttgg ttcattcttc tcactttgtt
    28801 cttcttcatg gacatgatga tggtgatttt ctgctacttt agacgtgatt ttttctttca
    28861 atacagcgtc tttttgaaaa taggcaagcc agacaccaat cacaagggca acaaaaaagc
    28921 tgcctatcac ccgccagctt gccatctgcc aagaattgcc aaatgcgata aaagtggaaa
    28981 aaatcacgat tggattgatg atcggagcag tcagcataaa agcaaacgct gtgtgaactg
    29041 gtacgccttt tttaacaaat tgatgaacga tcggtacgat gccgcattca catgaaggaa
    29101 agaaaaatcc taagatcgaa ccagtaaaaa tagagaagat acgattttca ggaagccatc
    29161 tcttcacacg ttctggggtc aaaaatactt gtaaggcgcc tgagataaga cagcctaata
    29221 atacgaaagg taatgcttca ataacgatcg ataagaaaat cgtactcatc tgcaaaaccg
    29281 aatttggaat aaaagaaaac atgagtttcc tcctttttat ttctagttca ttcgctttgc
    29341 aaatatacgc ttttattcaa aaaaaacaag ttgctctgta aatttttaca caactcttat
    29401 tttttaccaa agcacttcaa ttgacttttt ctgttattcc cattaaatta aggtctcata
    29461 catatcactt ctatcataaa agggagaata aaaatgaaac aaaccactaa cttgactgaa
    29521 gttcaagaca tactgcaaca gtcatccgtt gccgtcattt atctttcgat gcctaattgc
    29581 agtgtttgtc acgctgttcg tccacgacta gaggaacttc tcacacatta tgatattccg
    29641 gcacttcatt tggatgcatt tgaaacacca gaagtagcta gtaaatttga agtgctgact
    29701 gcgccagttg tcctgatttt tcatcaagga aaagaagtct ttcggcaagc acggttcatc
    29761 gactttaaaa aaatccgtta tctactagat gaactgacag cagaagatga ttccctttct
    29821 tatgaagaaa tcttccgaac aaaataatcg ttgtccaaac agaaaaaagc gattgaacaa
    29881 gtgaaagttt tgccttcatt tgttcaatcg ctttttattt ttattctgct gtattttcgg
    29941 aatgcgcatt tgcgtcagaa tgcaagaaat agatcaaggt ttgcaattca ttcgtcaaat
    30001 caacattttg gatcaataca tctgacggag cattcaatcg agcagcggta aagttcaaga
    30061 ttcctttgat tcctgcttcc gttaactgat tgactacttc ttgtgctctt tcagcgggta
    30121 tcgttaaaat tgctacttcg atttgttgga tcttgatttg ttctgacata tcttccattg
    30181 gataaacggg gataccgtct acgatgcgcc caacgatttc ctcattcaca tcaaaagcac
    30241 aactgatacg tatgctatta ctttgatgga atttgtattt tagtaaagca cttcccaagt
    30301 tccctacacc gatcagcgca acatttgtca attgatcgtc gtttaatgtc tttgcaaaaa
    30361 agtgcataag atcttctaca tcataaccat acccacgttt tcctaattcg ccaaaataag
    30421 aaaaatctcg acggatcgtt gcactatcta cttgaacggc ttcacttaat tcagttgaag
    30481 acactttttt ctttccagca ttatttaaaa tccttaaata gcgataatac aaaggcagtc
    30541 ttttagctgt cgcctttgga ataatatgat ctttcacgaa atgacctcca cgtattgtct
    30601 aattacatct ttgatttctc catcatagca gtatttatag gaaaaaatca attccattgc
    30661 tcagatattg tgaaatttta ttctttattc atttatttat catttctgca aatctcgcag
    30721 tataatggaa tttatggtag actactaatt gaaaaatagt tttttatttg aggagaattc
    30781 acaaagtaga aaagaggatt ccatgatatt attacaagcc aatcagattg ctcgccattt
    30841 tggtgcagaa gtcttatttg aaaacataca tttagaaatc agtacaggcg ctcgtgtcgc
    30901 tttagttggc agaaatggcg ctggaaaatc gactttatta aagatcattg ctggaattga
    30961 agcacctgat gaaggaaaga tcgctaaaaa taaaacagcg actctcggat acctcgctca
    31021 ggacacagga cttgattcca atgaaacagt ttggaacgag atgctgaaag cttttgaaca
    31081 agttagacaa atggaagagc ggatgcgcga gctagagatt ttcattagcg aaggtacgcc
    31141 agacacacct gcttatagtt cttatttaaa agaatacgac cggctgcaac acgatttttc
    31201 agatcgaaac ggctatggtt atgaaaatga aattcgttcc gtattgcatg ggtttaaatt
    31261 cgattcttca ttttatgatc agaaaatcga tactttatca ggcggacagc gcacacgttt
    31321 agctttagct cgcatgcttt tgcaacaccc tgacattttg attctagatg aaccaacgaa
    31381 ccatttagat atcgagacac tttcctggct tgagaattat ctgcaaagct atactggtgc
    31441 attattgatt gtttcccatg accgttattt tttagataaa gtagtcaatg aagtctatga
    31501 attaagcaga agaaaaatgc gccattacaa aggtaactat tcgaaatatc tagatttgaa
    31561 agcagaacag ttagcaagtg aatggaaggc ttatgaaaag cagcaaacag aaatccataa
    31621 attagaagac ttcgttgctc ggaaccttgt tcgcgcctct accacaaaac gagcgcaaag
    31681 ccgcagaaag acattagaaa aaatggaccg gctggatcgt cctcaagggg atgaaaaaca
    31741 agcaaacttt ctattcagta tagaaaagcc ttctggaaat gtagtcttgc aaatcgaaga
    31801 tgccgctatc ggatatgata atgtcacctt atctgaacca atcaatctag atatccgtcg
    31861 tcaagaagcg attgctctag taggtccgaa tggtatcggg aaatcgacgt tgctgaaatc
    31921 aatcattcac cagatccctt ttatccgtgg aaaagaaacg ctagggacaa atgtcaccat
    31981 cggctattac gatcaaggtc aagctgatct gcatacaaac aagacgatcc tagcagaatt
    32041 gtgggatgag catcctacca ctccagaaaa agagatccga agtgctttag gtgctttctt
    32101 attttctggc gaagatgtag aaaaaccaat ccctctgcta agtggcggtg aaaaagcacg
    32161 ggttgcttta gccaagttag cgatgaacaa agaaaatttt ttgattttgg atgaaccaac
    32221 aaaccattta gatattgaca acaaagaagt attagaaaat gcattgatcg attatgacgg
    32281 cacactgctt tttgtttccc atgaccgata tttcatcaat cggatcgcga caaaagtcgt
    32341 cgaactctct gaaaatggca gcaaagtcta tctaggagac tatgattatt atttagagaa
    32401 aaagcaggaa gaagaagaaa tcgccgaatt gttggcacaa gatgagccag aatctgctcc
    32461 tgctcccacg aacaaatctg attattacca aagcaaagaa cagcaaaagc ttatccgtag
    32521 ccttcaacga aaaattactc aggttgaaga agaaatggcg cggatcgatg aatggatcga
    32581 tcagttggaa caagaaatgg tcgcgccaga aaacctggaa gatcacgtaa aactcaatga
    32641 gctgaaccaa gatttagaag ctgctagaca agatcaagaa acaaaattga cggaatggga
    32701 agaactaagc ttggaattag aagaatatga aaattaacga aaaaaccaaa acagaaacca
    32761 ttcatgctgt tttggttttt ctttttttat tttatcaaac aaaaattcca tatttacctt
    32821 tttcaccaat aagaagacca attactagtc attctcacta aagcttctaa ataatagtaa
    32881 tcaccccaaa tacaaggtgt atttacccca ttatttttgg gcttattata aacagactgt
    32941 gttaaaaagc catcttcttc ttccgttgta gcatatcttt ctttcaattg tcgcataatt
    33001 ttccaaacag cagactggta taagttcttt tctgttttgt ctaatagacc tgattctatt
    33061 aattctaata atccacaaac catgatcacc atactagatg tatctttttc ttctctgcta
    33121 gaatcatcaa agatcaaatc ccaataacaa atggtatctt ctggcaaccg attcataaaa
    33181 taattggcta atattttgct taaatcaaaa tatttttgtt ctttcaaata acgataactt
    33241 aatgcaaaac cgtaaatccc ccatgcttgg cctctagccc aacaggaatt atcactataa
    33301 ccttgatgcg tacttccata tcttggtttt ccatctttga catccatata aaatgtatga
    33361 taagttgaac catcttctct tactaaataa tctgctgcat tttgaatatg agcctgagca
    33421 gcttttttat atttttgatt tccagtttct tcactcgccc aatacaataa tggaagattc
    33481 atgcaacaat ccatgatcat tctaccttgt tgtgaaggat cactaggatc tccccaagct
    33541 tgtataactc ctgccttctc ccaaaaacgc tgaagtaatg tttctgcagc atcaattgaa
    33601 gcttccttca tttttcgatt tcccgttatt ttataatctg ctacggttga taaagaatac
    33661 aaaaaaccaa gatcgtgatg attagtgata atttgacagt caatcctgtg gataaaatct
    33721 atatctgctt tttcagctgt ttgttgaaat aaaggatcac ccgtcttctc aaataacaac
    33781 caattaattc ctgtccaaaa tccttctgtc caaccaatat tgtctcttaa tggataacgt
    33841 ccttttatag gacactcgct tgggaattga tactgactgt tactgatacg tttgattaac
    33901 ctctccgtgt gtctgcgaca acgctcgatc tcattattcc attctttttc cattaattct
    33961 ttataacatt catttatcca tttttctgga tgtattatct cttcaaacat cactttatca
    34021 agcatttccc caccagcctt tagcataatt ttagtctttt tttattgttt ttattttgga
    34081 tagttagccc ggttgtccca tcaaaatcaa ttttgggacg attgttccat acttctaaac
    34141 tataatcccg atcttgatga gcaaccactc ctgttccgag ccaatgggtt ccttttccaa
    34201 atgtaccctc taatccagga ataaaagtgt tttcccatgt catcaagttt gtattgggca
    34261 aacccataat agctgtggat tttcgtttaa tagtatcgtc acttaaacag ataatacctg
    34321 aaaagccagc ttcactcacc ctatagtttt ccttgtcttc ttctatgatt attccttctt
    34381 tctcaggaga acattcaata gcaaaccctg tctcatatag aacatattct gtatctaatt
    34441 ctactttgtg tacgcgaatc tgccagcctt caaaaggaat aagccaagtt ctgatttttg
    34501 cctgtaaagt aggttcccat gtagaggcaa gtacctcttt cttacataca atgtctcgta
    34561 cattatttcg aacgatgaac tgtccatagg ttttgatgct ctcattctta aaactggtcg
    34621 tgatatatgt gtccgctcgc tgaatcccta gcattgaatc cattgccatt tctgctttca
    34681 attgattcgc tcgcggtatc gaaaaaccaa attgactaga ataagcaaat tttaaatatt
    34741 tttcattggt caatgtatgg aaatttggcc ctaattgtcc cgcattcaaa aaaaatgtat
    34801 gtccattatc atgttgtatg agcaaattgg cttctgaaag aagtttagtt ccttcacgaa
    34861 taggcatagg catttcttta gccgtccaat actcatggtc ttcaggtagc gccaacaata
    34921 aaaaaatctt attcagccat aatggagata acgtactatt atagggttca gtcaatgtca
    34981 attgagggta ggtataacca atattcagga tacctttttc atcaaatatt tctttttgca
    35041 tccaccatct tatattccga ttgactaacc ccttgatttc acctaattta aaaggcaaaa
    35101 gatcggccca caacatagct gaccaaaagc ttgaaactgc aaaacgataa atcatgcttc
    35161 gaccaaaagg cacatttgca ccatcttccg caaaaaaatg aatatagtct ctagaaaaat
    35221 caatagctcg gttcttatat atctggctaa tgggcttttc tggttctagt acgctataaa
    35281 tcaaaccata ataatgaaat gcaaaaggat tataataatc ttggcgacct cttgaactgt
    35341 ctcgatacca gccattttga agataacaat tttctatatt atcaaatgct tctttcagtg
    35401 ctgattcttt aactggataa ccaaattttt ggataattgt tccgacaatt atcttaaaaa
    35461 attgccaatt accatctgca cattcatatt caaaaatttg cgagaaccaa tgaataatct
    35521 ggcttttttc tcctgtatct aattttttcc ataataattg ttcatgatga tataacgcta
    35581 ataacattgc gggcatttct accatacgct ggtctctttc atgtatttct ccccaataat
    35641 cgggatggga aggatctgtt ccagctttta ttcctgatag aattaatttc aaccatcgtt
    35701 catctaattg gtcaattgcg ggccccgctc cccataaaac ccgagagaat ccttccattc
    35761 ctgcaatttt atttccatat cctacccctt gatgtatata atcaatttgt gcgccacttg
    35821 ctcgataaaa cggctggagt ggttgattta tctttttaaa taagtcaaag taatcctgtc
    35881 tggtccttaa tagtggtact ggatgtttca ttgtattttc tcctcttttt actaccaaat
    35941 agctaattcc gactgttcta atcgaagtaa gccttcaata aaaaaataat cagaatagat
    36001 cagtggcaca ttataagtca gttctctatg gtatgcttct gctccgcctc caatgatccc
    36061 atcttcatcc atattccaat ttgcatagtg cttttctgtc ttttggagta tattgatagc
    36121 agcttcatga tacatttttg atcgtacaga ggaaacatat cctgctaatt ccagaagacc
    36181 gcatgcagcg catactccag cactggtatc aatattcgaa tcttttacag caaaatcaat
    36241 gagaggaata taaccagttt tttcgatttt ctgaataaaa tttactgcta tcttttctgc
    36301 tatagctaga tagtacggat tttttgtgta acgataactt aacgcgaatc catataaagc
    36361 ccatgcattt cctctgctcc aacaagaatt ctcattgtat ccttgcccgc caagtaaccg
    36421 aatatatttc cctgattctg catcaaattc tgcgatatgc ccggttgatc catctttcct
    36481 cactagatac ttagctgttg tatctgcatg ctgccgagca attttagaga acctcggatc
    36541 accagtttcc ctcgaagccc aatacagtaa tggtaaattc atcatactat caataatcat
    36601 ccaaccaggt ttatggtcat tccatgctac taaatagttc cctgaaatat tatagcgtcc
    36661 agcaagtacc gtagctgcat gtaaccctag ttgtcgacta tgtttgtttt gagactgttg
    36721 ataatgtgca atagccgatg gtaagaataa aaaacctaca tcatgatgaa cgtctgaaaa
    36781 atggtacagg gcagattcta ataattgttc ttgttgtatt gctgcctctt tgaacttttc
    36841 tcgttttgtc ttgtgaaaga gctgccataa aataccactc caaaaaccat tggtccacca
    36901 gtcgattcct ctgtccttta ttacgtctgt atattttccg ttgtaagaaa tatatggaat
    36961 tttattcccc attctgtcta attccgcatc tatcttttca gaagctacat tcaaaatttt
    37021 tcttgaccat tccttcgtct gttcattcat ggatatcccg ccattctttt aatttttatg
    37081 aatgtggagt tgctcctaga acgaactaga tggttaacat tcgttatttt ttttacttat
    37141 ttgcagctcc aatgtcattc tccttaaatt tagactgcgc ccacacataa atcaagacca
    37201 ttgggatcac tgaagttgct aaaattagca taattgcgcc ataattaatt ggctcacctg
    37261 tactagaaac cgtcatattt ttgataatta agggaaccgt atatttatct ttatctgaaa
    37321 gcatgactaa tggaaataga tattgattcc aacttcccat aaagataagt aaaccactag
    37381 taataatcgt aggtgataaa atcggtacag cgattttcgt aaaaatcacc cactcattgg
    37441 caccatcgat tctggcagcc tccatcagtg ctgtaggaaa ttgactgcta tattgtcgca
    37501 tcataaacac tgaagttgta ctggcaatcg aagggatgat cacacctgca taagtattcg
    37561 ataatcctaa acgactcata atgtaaaata aaggaatcaa tgtggtaaaa aaaggcacca
    37621 tcctagatag cataactgta ttgaacaaca gtttattccc cataaaacga tactttgcta
    37681 atgcataacc cgccattgta gaactggctg tagctaaaac tgtactgatt acagcaataa
    37741 caatcgaatt ccaaataatc aagggataat tatatctcgc catgatggct tgccagtttt
    37801 ttaatagcat atccccaaac ttgaaattga ttccgccact taaaatttct tgattacttt
    37861 gcgttgaagc tacaatcata tataaatatg gcatcaaaaa tattaaggca ccaaacacaa
    37921 taatgacgta cgtaaaaatt ctgctttgta tcttttgttt agtcattttt atcacctccg
    37981 aatttaaatt gaatagccga taatataaca gatacaaaca ccatcgtcca ggcaatagca
    38041 gaggcttttc caaaatcaac catttcaaaa cttgttttat ataaattgac tcctacgacc
    38101 aaagctgaat tttctggtcc attggaagtc ccaaagaata tatttgggat attgaaatta
    38161 cccaagccat tgatagtggc taaaactgtg gccattaaaa tgattggctt tagcatgggg
    38221 atcgttaata aaaagaattg ccgaaatttc gatgctccat ctatttcagc actttcgtat
    38281 atctctttag aaatcccttg aagaccagct aaaaataaaa tagtgttata tcctacacta
    38341 agccaaatca tcgtgatgat cattgatatt cgtatccaaa aaggatttgt taaccactgt
    38401 acaccgttaa ttcctagcgc attcaaaaat ccattgaata acccagtcgg attgaaaaaa
    38461 gtcatgaata cggaagctac agccaccgtt gatgtaatgg ttggaatata atagacagtt
    38521 ctaaaaaatc cttttccttt taactgtgct gaattcaata aaacagcaaa gtaaatagct
    38581 aatagcatcg ttattggaat tgatcctgcc atgataatca aaacattcgt gtaagattta
    38641 ataaacaatg gatctcggaa gatagctaga taattgtcaa atccaacaaa ttcaaatgag
    38701 tagccaattt tcataaaact aaaagcaaac gagataatga tgggaatcaa aatcgtcaca
    38761 ataaagaaaa taacaaaagg tgtgaccaat ccataagcac tttttttatg agacgtatct
    38821 gttgcttttt tcttgctgtt ctttattgtc ttgcttggta acgaagattg catcatcgtt
    38881 aagtcctcct aatatttttt aatcaccagt taattttacg ccgtacttat gagcaaaatt
    38941 ttctgcttct tttagatatg aattttcaaa atcacctgtt tgccaatagt tatatgcagc
    39001 ggtagtcaaa taagacgtag catctgaata agtggctggt aaaacagtat ttgatgtatt
    39061 ttcattgatt ttattcaata gttcatagta tttttgtcct ccttagtagt caaacttttt
    39121 ctcagctgct tctgtttgat aggccaatga gttagatgta gccacgccca gctccagtgc
    39181 gttggctaag cattcttcat ctgtcagcat aaatgtaaga aattgttgtg ccacttgtgg
    39241 attttttgca tctttaggga cataaaaact tgatcctcct gatactgcac cttttcctgg
    39301 attttctttt gaaaaaggaa tcagtggagc catgcgccat ttgtcagatg catcaggaaa
    39361 attcaaagac atattcgtag cataccagcc gccagcaaca aacattgcag attcttgaaa
    39421 tgctttctca gcatcttgtg caccgtagaa atgtacaata tctgagtcga tcatttcttt
    39481 gattaacttt gcagcatgtt tcgcttcttt agtattcaga cggatatttc cttcttcatc
    39541 aagcaaagat tgattctgct gggctagcat gttgataaac aattcttggt ccccagattc
    39601 tggcaaggca attccaaaac taccagtttt ttcttttaat gcttttgcca cctggatata
    39661 ttcttcccaa gaactgattt cttcaaaatc tacacctatt tcagcaaatt tttttggttg
    39721 ataataacaa agtgctgggg taaaatcact agcccaagga ataatcgttt cattactcgt
    39781 ttgttgacgc aaaatattca atttttgatc ataaaactga tcgccatatt tgtcataaaa
    39841 tccatattca tcagcactat aaaaagcatc ttgaaatttt tccagcacac ctgctgctga
    39901 agtgtctgcc atggagataa tatccggcat atcttcgtgt gttaccaatt ttggggtgat
    39961 catttgtact gttgctgttc ccgaagctac attcacaatt tttaagtcaa tatttgctcc
    40021 agttttgcgg ttatagattt ttgcagcatt ttcaacatag tttgtcagtt tgccgtttgc
    40081 gacatatact tctaatgtag tggctttttt tatcattgga tctgcgatgt ttgctgttac
    40141 ttcttcgtta gtttttccac atgcacttaa gctgaataat agggtaatgt ttagcaaagc
    40201 tatcaccaat ttgtatcttt ttttcatgtc tatcccttct ttcttttcaa gttcttatat
    40261 aaaacgcttt cacttatgta tattaacgga attagtaaca caaatattta tataaaaaaa
    40321 tattttactt gtcgttttgg cttttcattt ttgttataat agtcattaat tataaataga
    40381 aagcttttta tataaaaaga gaaaggaaag tggttctttt gttaacaaac aaccaatcct
    40441 ccaatcttgt caaaaaaaca ctaacctatt ccatagatac ccatcttcat ttatctgctg
    40501 ctccctattt tcgactacta ataaatgaaa gcgctaattt atatgctgaa tttgcacata
    40561 aatctataga actagataat ccttcccttt tattcattga taaacagcaa gaagtccaaa
    40621 tcttttctac tcaaaaggaa acacaaactt ttatcattat ccagttttat tttgatgagc
    40681 aattattagc acatattacg ggacaatctt tttctataga tcaagaaata acatctatca
    40741 ttacttttct gaaaaatgaa cagttcaaaa ttgattatct aaaacatagt aagccagcta
    40801 tttcctatga tcaggaaatc aattcttttt tcatactatg ccaacagatg agcattcatt
    40861 tgctttcctt attagttact tattgtctgc aaaaaattca attgtcttcc ttaccaaggc
    40921 attatcaaca ggaacttgct ttattgacaa taccttcaaa tgagaaagtc attcctaaaa
    40981 aaaagcatat tctttattca gatgaattag ttgatccaat catacgatat ttagaagaac
    41041 atctttcaga aaattttttg cttgaacaat tagcccaaat tttttttatc agtccagcca
    41101 gcttaaaaaa acattttaga aaagtgaatg gaaaaagtgt tattagctat tttaaagaac
    41161 taaaaatgga aaaagcaaaa cgtctcgtgc tagaaaatga attaacctat acagaaattg
    41221 cagatatatt aggatttcat tcgatccatc atttttcttc agcttttaaa aaatatacag
    41281 ggttatcccc ttcaaagtat tatctatcct taaaatagaa aaaaattttt aggttttatc
    41341 cacaaattat ttctctatat tttctgtcta ttcaaaataa acaataattt tccggcacaa
    41401 ttatttgctg ttttttacca taacatagta cgatcaacgt agaaaaggag atgtttttat
    41461 gaaagttgtt gtcattggcg gtgtagctgg cggtccttca tttgccactc gtttccgtcg
    41521 attgaatgaa gcgcacgaaa tcattatcta tgaacgcgga gaaaatattt cttatgcaag
    41581 ttgtgctttg ccttattatt taggaggtgt gatcactgac cgtgactcgc tgatcgaacg
    41641 tacaccagaa atattgaaaa caaaaaacaa catcgatgtc tttactaaac acgaagtaac
    41701 agcaatcgat ccttctacta agcgattaac agttaaagac ctatccacaa atgaagaaat
    41761 aaaaacagat tacgataagt tgatcatctc ttctggtgct agaccagatt atccggatat
    41821 tcccggagtt tttgaagcag aaaacggctt tgtcctccgt agtgtgacgg atgcggatcg
    41881 aatcaaatcg ttccttgaag aaaaaaatcc acaacatgtc gtcattcttg gtgcaggtgt
    41941 tatgggtctg gaattagctg agaatttcaa gcatcgcggc ttagacgtga ctttaatcga
    42001 tcaattgcca caagtcgctt tcccttatga tccagaaatt gctaatttag tttatgacaa
    42061 attgctgaaa gaaggattag ccgttcattt agaaacaaga gttactgaga tccgtgataa
    42121 aggtcgagaa atagtattat cagatggctc cgtcctttct gctgatatgc taatttttgc
    42181 tgttggtgtt tctccgaaga atgaagtggt gaaagcaacc ggcatacaat tatcagatac
    42241 aggtcagatc attgttgatg accagttaca aaccaatctg ccggacatct atgcgattgg
    42301 cgatattatc gaaacgacta gtgtagtgac tggtcagccg atccaaagta tgctttccag
    42361 tgcggccaat cgtcaaggac atatgctggc agatatttta aatggtacgc ctatgcgcta
    42421 tcgcggatat attggtgcag gtgtcgcaaa aatctttgat catacagcaa gttatgcggg
    42481 aatgacagaa catgaattaa aagcatcagg cataacaaat tataaaactg tttttatcac
    42541 tccctttgac catgcctatt tctatccaga agctacaaga ttaaatctaa agctgatttt
    42601 tgatgcagat agcggtcgta ttttaggtgg acaagcattt ggagaaaaag gtgtcgataa
    42661 acggatggga gaactttctg tagcaatcac cggaaacttg acagtctttg atttgcctga
    42721 tttggagttg ccttactctc caccgtattc tactacccgt gacccgttga atatagctgg
    42781 ttatgtcgca atcaatcaaa tgacgaatat cgtagaaacg atcaaagcaa gtgatatacc
    42841 cgaaaacgat ttgaaagaag cgttcttttt agatatacgt gaacctaata aagcaccttc
    42901 tggcagtatc tcggcaacaa aaaatattcc aatgaatgag ttacgagatc gaatcaatga
    42961 aatcccaaaa gataaaaaag tttacattac tttcagaaga ggattgaata cttatacttc
    43021 tgcccgaatc ttggcaggtt tgggtatcaa agcgattttg attgaagaat aaaaaaacga
    43081 ccgcggtggg tagccgcggt ctaggagtta tttttacttg aggagtaaaa atgaaaaagt
    43141 atatagggtt gttggttggt atgtatatag aatagaagat aaatgtgaag aaattgtgca
    43201 ttatttcttc acatttatta ggttatagtt taagaaagtt taaacattgc gcaatttaat
    43261 tattttagct gtttcttttc ataaaaaaac atgttgtgac tctgagacaa atcatagtcc
    43321 atagtgatgt ctctagagta actacgccta atattacgag gcatcatatt aatctatcag
    43381 cactagtacc taaactagca tccctttcga atattttttc ttatataata aagtttgctt
    43441 tcactcctct aatctcacca aatcgactgg cttgcgttgt gtgatactag gaactgattg
    43501 gtttagaacg acttttctat tgtaatcata tttttcgcca tcctcgttgt cttctaatcc
    43561 ttcaatgaat gatccctaat gtgtgtatta aataatttaa tttttttgtt cctatttatt
    43621 taac
//
LOCUS       DACCYH010000014        43605 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.014, whole genome shotgun sequence.
ACCESSION   DACCYH010000014 DACCYH010000000
VERSION     DACCYH010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.014"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..640)
                     /locus_tag="IVY85_000725"
     CDS             complement(<1..640)
                     /locus_tag="IVY85_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043759.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLY"
     gene            complement(819..1538)
                     /locus_tag="IVY85_000726"
     CDS             complement(819..1538)
                     /locus_tag="IVY85_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8043760.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(1669..2820)
                     /locus_tag="IVY85_000727"
     CDS             complement(1669..2820)
                     /locus_tag="IVY85_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP8043761.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(3037..3777)
                     /locus_tag="IVY85_000728"
     CDS             complement(3037..3777)
                     /locus_tag="IVY85_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8043762.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3781..4104)
                     /gene="rsfS"
                     /locus_tag="IVY85_000729"
     CDS             complement(3781..4104)
                     /gene="rsfS"
                     /locus_tag="IVY85_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP8043763.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(4161..4754)
                     /locus_tag="IVY85_000730"
     CDS             complement(4161..4754)
                     /locus_tag="IVY85_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8043764.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4738..5385)
                     /locus_tag="IVY85_000731"
     CDS             complement(4738..5385)
                     /locus_tag="IVY85_000731"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP8043765.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(5398..5709)
                     /gene="yhbY"
                     /locus_tag="IVY85_000732"
     CDS             complement(5398..5709)
                     /gene="yhbY"
                     /locus_tag="IVY85_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP8043766.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(5725..6834)
                     /gene="yqeH"
                     /locus_tag="IVY85_000733"
     CDS             complement(5725..6834)
                     /gene="yqeH"
                     /locus_tag="IVY85_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP8043767.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6831..7358)
                     /locus_tag="IVY85_000734"
     CDS             complement(6831..7358)
                     /locus_tag="IVY85_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP8043768.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(7538..8323)
                     /locus_tag="IVY85_000735"
     CDS             complement(7538..8323)
                     /locus_tag="IVY85_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP8043769.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(8316..9185)
                     /locus_tag="IVY85_000736"
     CDS             complement(8316..9185)
                     /locus_tag="IVY85_000736"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP8043770.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(9186..10565)
                     /locus_tag="IVY85_000737"
     CDS             complement(9186..10565)
                     /locus_tag="IVY85_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP8043771.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(10573..10992)
                     /gene="fabZ"
                     /locus_tag="IVY85_000738"
     CDS             complement(10573..10992)
                     /gene="fabZ"
                     /locus_tag="IVY85_000738"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8043772.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(11017..11493)
                     /gene="accB"
                     /locus_tag="IVY85_000739"
     CDS             complement(11017..11493)
                     /gene="accB"
                     /locus_tag="IVY85_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP8043773.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(11496..12731)
                     /gene="fabF"
                     /locus_tag="IVY85_000740"
     CDS             complement(11496..12731)
                     /gene="fabF"
                     /locus_tag="IVY85_000740"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP8043774.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12750..13487)
                     /gene="fabG"
                     /locus_tag="IVY85_000741"
     CDS             complement(12750..13487)
                     /gene="fabG"
                     /locus_tag="IVY85_000741"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP8043775.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(13499..14416)
                     /gene="fabD"
                     /locus_tag="IVY85_000742"
     CDS             complement(13499..14416)
                     /gene="fabD"
                     /locus_tag="IVY85_000742"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8043776.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(14523..14747)
                     /locus_tag="IVY85_000743"
     CDS             complement(14523..14747)
                     /locus_tag="IVY85_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8043777.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14800..15762)
                     /locus_tag="IVY85_000744"
     CDS             complement(14800..15762)
                     /locus_tag="IVY85_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP8043778.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15772..16212)
                     /locus_tag="IVY85_000745"
     CDS             complement(15772..16212)
                     /locus_tag="IVY85_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8043779.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            16518..17483
                     /locus_tag="IVY85_000746"
     CDS             16518..17483
                     /locus_tag="IVY85_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8043780.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(17540..17770)
                     /locus_tag="IVY85_000747"
     CDS             complement(17540..17770)
                     /locus_tag="IVY85_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP8043781.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(17856..18743)
                     /locus_tag="IVY85_000748"
     CDS             complement(17856..18743)
                     /locus_tag="IVY85_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP8043782.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(18832..20055)
                     /locus_tag="IVY85_000749"
     CDS             complement(18832..20055)
                     /locus_tag="IVY85_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8043783.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(20072..21151)
                     /locus_tag="IVY85_000750"
     CDS             complement(20072..21151)
                     /locus_tag="IVY85_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8043784.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(21282..23009)
                     /gene="ptsP"
                     /locus_tag="IVY85_000751"
     CDS             complement(21282..23009)
                     /gene="ptsP"
                     /locus_tag="IVY85_000751"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP8043785.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(23009..23275)
                     /locus_tag="IVY85_000752"
     CDS             complement(23009..23275)
                     /locus_tag="IVY85_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP8043786.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            23754..25988
                     /locus_tag="IVY85_000753"
     CDS             23754..25988
                     /locus_tag="IVY85_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8043787.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            26136..26456
                     /locus_tag="IVY85_000754"
     CDS             26136..26456
                     /locus_tag="IVY85_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8043788.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(26661..26885)
                     /locus_tag="IVY85_000755"
     CDS             complement(26661..26885)
                     /locus_tag="IVY85_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP8043789.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(27083..28663)
                     /locus_tag="IVY85_000756"
     CDS             complement(27083..28663)
                     /locus_tag="IVY85_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP8043790.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(29064..30440)
                     /locus_tag="IVY85_000757"
     CDS             complement(29064..30440)
                     /locus_tag="IVY85_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8043791.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(30568..31686)
                     /locus_tag="IVY85_000758"
     CDS             complement(30568..31686)
                     /locus_tag="IVY85_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8043792.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            31828..32262
                     /locus_tag="IVY85_000759"
     CDS             31828..32262
                     /locus_tag="IVY85_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8043793.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(32340..32873)
                     /locus_tag="IVY85_000760"
     CDS             complement(32340..32873)
                     /locus_tag="IVY85_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP8043794.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(32967..34145)
                     /gene="mutY"
                     /locus_tag="IVY85_000761"
     CDS             complement(32967..34145)
                     /gene="mutY"
                     /locus_tag="IVY85_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP8043795.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(34138..34935)
                     /gene="recX"
                     /locus_tag="IVY85_000762"
     CDS             complement(34138..34935)
                     /gene="recX"
                     /locus_tag="IVY85_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP8043796.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            35042..36424
                     /gene="rlmD"
                     /locus_tag="IVY85_000763"
     CDS             35042..36424
                     /gene="rlmD"
                     /locus_tag="IVY85_000763"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8043797.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(36598..37176)
                     /locus_tag="IVY85_000764"
     CDS             complement(36598..37176)
                     /locus_tag="IVY85_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP8043798.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            37498..37968
                     /locus_tag="IVY85_000765"
     CDS             37498..37968
                     /locus_tag="IVY85_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP8043799.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(38076..39302)
                     /locus_tag="IVY85_000766"
     CDS             complement(38076..39302)
                     /locus_tag="IVY85_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP8043800.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(40056..40256)
                     /locus_tag="IVY85_000767"
     CDS             complement(40056..40256)
                     /locus_tag="IVY85_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8043801.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(40390..40998)
                     /locus_tag="IVY85_000768"
     CDS             complement(40390..40998)
                     /locus_tag="IVY85_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP8043802.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(41083..41553)
                     /locus_tag="IVY85_000769"
     CDS             complement(41083..41553)
                     /locus_tag="IVY85_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP8043803.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(41601..42515)
                     /locus_tag="IVY85_000770"
     CDS             complement(41601..42515)
                     /locus_tag="IVY85_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8043804.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            42661..43464
                     /locus_tag="IVY85_000771"
     CDS             42661..43464
                     /locus_tag="IVY85_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP8043805.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
BASE COUNT        12496 a         9208 c         7358 g        14543 t
ORIGIN      
        1 tatataaatc caaatctgtt tcaggatatt ttaatttgac aagcgaaata atcattttca
       61 gttcttccat gttttctgga tattctgggt caaatacctt gctatgacgg ccaatcccgc
      121 cattgtcgat caatcggtaa attcgattcc attcaatgaa ttggtcaatt gattggaaat
      181 tgctgattgg atagccgcca caatccaaaa tctctgatac acgaaatgct aggtatgcac
      241 tatcgatcac tttatttttt ttcgtgtcta tcttctcatt tctttcgata agaatttttt
      301 caggacgatg tttcaagctc cactctaata gtagaaagaa agcctccatc cgatactctt
      361 cttcaaataa ccgaaaggct gaatcagaac gacgaaaaag cagcggaatc agataatttt
      421 gtctataaat cagcattggt gcgcggtcca ccatacactc taaataacgg ttcagatacc
      481 ctgtaccgac atagtttcct aatcgatagc cttcaacgcc gaatcttgcg tatttttgcg
      541 ctaattttga acaatcagtt acgttcacag ctaactcttc tactttgcgg taaccgctta
      601 agatacgttc aaaaggttct aaagtgattt ttgcttccat attcttcccc gtccatcttc
      661 cacatacttc atatcatttc acatgatact tatgctctca tcataccaca ggttgaatta
      721 gaaaagaaaa taaatcacac aaaaaagcaa gacaatctgt tagtgagatt gtcttgcttt
      781 tcgaaaaaga ctgaatactt ttcgttaaaa ctggttgctt agtcttctaa tgccacatta
      841 tggaacacat gctgtacatc ttctagatct tccagtgcat caatcatttt ttcaaatgtt
      901 tcttgatctt ctggagatag tgttacctcg ttttgaggga tcatttggat ctcagcgatt
      961 gaaaaatcag tgattccttt ttcttttaat gcttcttgta tactatggaa atccgctact
     1021 tcaccgtaaa cgatgatttg tccttcttct tccattacat cgcgaacatc gatgtctttt
     1081 tccattaggt attcaaggat ctcgtcagcg tcttctcctg caaaagcaaa gattgctgta
     1141 ttgtcaaaca tgtaagaaac cgctccggaa acacccatgt tcccgccatt tttcccaaat
     1201 gctgcccgta cgtctgctgc tgttcgattc acattgttcg tcaatgtatc aacaatcacc
     1261 attgagccgc ttggtccaaa tccttcatag cgcaattcag agtatgtttc atctcctgca
     1321 ccttttgctt tttcgattgc tcggtcaata atatgttttg gtacattata agttttagcg
     1381 cgatcaatga cgaaacgtag tttttggttt gcatgcggat caggatcacc tgattttgct
     1441 gccgcgtaga tttcaatccc aaattttgca taaatccggc tattattagc atctttagca
     1501 gttttctttg ctacgatatt tgcccattta cgtcccatat tttcacttcg ctttctgatt
     1561 tgatagtttc ataacgcaaa aacgtcctgt tccattatac ttgtgaatca cgattctgtg
     1621 aagctttttt tctttcttcc ttcattcttc tcatttttag tcaattatct atactcggat
     1681 tgggatttgc ccgaaattct gttccggaat attgcctccc aattgataga tttgatcact
     1741 tctaactaac agttccgctt cttgtgcctc tttttttccg attcgggaat aaatagaaat
     1801 ctcttttttt tcttttaaga atcttcttcc agcatcggta aatccaagta gtcgaagata
     1861 atttttattt tgttggacag gaatctcttg ctttttgata ttcagcaaga cataacaaga
     1921 taatcgttgg atccgtgtcc atgtatatct ttttgttttt acctttgaca caaaatcctc
     1981 aaaactatca gcagttgcag ctgctgtttt catgcgcgat tctattcctt cggtcatctg
     2041 atagatatct gtcagttcca gcaaagtaga agataacaac cgataacgca aatacggcca
     2101 ataagattcc caagtctgca gcgaacaagt tgaaagatct tcttctgtct gaggcggcac
     2161 aagtccagct attctttttc cttcttctaa cgcatggcga atggctgttg cactggctat
     2221 cctctgatct gtgatgattt tttcatgata gcctgcactt ttacgtcgga tcggatgtaa
     2281 ttccataggg tggacatact tggcattttc ctttgcataa cttaatccca agatatgatt
     2341 tggcgaagaa aaatcaagcg taatatcggg atacagactg cgaaaaacct cagtcatttt
     2401 ttgcggataa ctatacgttt gatcagacag ttcttgaaat gcctcttcta tcctatcttg
     2461 attctcttgg acaaagcgtc cgaaactgct gtaatcaaac gaagtctctt cttctgttcc
     2521 aaaacacaaa ctgtcacagt ccagtgtttg caagattttt actgcacctt tagcaaaata
     2581 gtcagctgac tgaaccgccc attcgacggg aagctccact actaaatcca caccattttg
     2641 taaagcttca ttcgcgcgat gccatttatc gatgatagcc ggttcgcctc tttgcaaaaa
     2701 attcccgctc attacagcaa tgacgacctc tgctcctgtt atttttcgtg ccatttccac
     2761 atgatagcga tgtccattgt gaaaaggatt gtactctacg ataatcccgc agcttttcat
     2821 gcgcatcctc cttcatagtt atcgataaaa aagaaataga gacaagtaaa aaaaggcaaa
     2881 aatcggaaaa aatacccgaa ctatataaac tattctcgca aacacaatac ttctgcgacg
     2941 aaaagctgca ctgcaagagc aatgcctata tcgttcggat atataattta tgttgaagat
     3001 ttattctttt cttgttaact cgagcaggca aaaccgctag tctttgtatg ctacaaagaa
     3061 ccagcgcttt gatttctctg atgctgcttc gtctgtaaaa tcagaaaatg cttcgacttt
     3121 cgaaaagccg ctgttttcca gcattcttaa ataactttcc aatggatagg tacgctcttg
     3181 atgtagttca tcttcacgaa taaatcgacc agacccttct tgttcttcaa cgaaaaaggt
     3241 aagaaaatgt tcgatgctgt gtttctcgct tcctggataa ctatcccaca aaaaagcaaa
     3301 attatcccct tgataatgat agctatattc tggaaaaacc tcatctactt gataggtgga
     3361 gtgtacgtca aataaaaatc ggccattttc ttctaacgct tgatagactt catcaaacac
     3421 ttgctgtacc tcttgtctat ttttcatata gcacagcgaa tcagaaaaac aggtaattgc
     3481 ctgatattgt ccgatttctg ataaatcgag catattgcct tccacaaatt ggatcgatgc
     3541 gtcttcttca tatgctcgct ggctggcaat catcaacatc tcttctgaaa ggtctaaccc
     3601 tgtcacttta tatccatctt ttgcaaaggc aaccgctaat gctccagtac cgcatgccag
     3661 ttccaaaata ttttttgtgt cttcttgtaa atgacgtttc gaaaaagcta accattgctg
     3721 ataaagtgtt tcgtccatca cttcatcgta aatgaaagca aatgtttcat aagccatatg
     3781 ttaatccaac cattcactaa gatcgactag cggagcatct gaccatagtt tttccagatt
     3841 atagaaattt cgttctggcg catggaaaac atgtacgatc acatctccaa gatcgatcaa
     3901 tacccatttt ccgccttctt ttccttcaat acgttttact tcatatttgt tttcttcttc
     3961 tttatcaatg atttcttctg taattgcgtt gatttgacgt tcgctatttg ctgaacagat
     4021 catgaaataa tctgccaaca aggaaacatt tcgaacgtct aaagcgataa tgtcttctgc
     4081 ccgttttgaa tctgctgctt caatcgcaat ttttaattgt tctttactat ctatgatgat
     4141 ttcctcctaa tatgagcagc ttattttttt gctacccagt aattatatgt ttcgattgtt
     4201 tttggataaa tctgttgttc ttgcgcaagc aaatgggcca atgtatgttt tgtttcaaat
     4261 gctacagctt cgtctagatc agcccatgca atggctcgtg catcttgtac cccaggaaaa
     4321 tctcgacctg gttcaatata gtctgctaca taaatgattt tgtcaaggag actcatctta
     4381 gcagccccag tcgtatgcac ccggattgca tgaagaatct ctgcatcggt gattcctaat
     4441 tcccgctcta caaacgaagc gccaacaaga ccatgccaga ttgcattatt ataattcaat
     4501 aactccggat caaaaccgtc tcttacgatt accattttga actcctcatc tggtcgttct
     4561 tttgcataat catgtgtcaa tgcagcaata ctagcttttt caggagaagc accgtatttt
     4621 ttggcaagag ccacggctgt ttcttctact ccaagcacat gcttgaagcg ttgttcgctc
     4681 attctcatct gtattttttg catcaggttt tctctagaat aagcagtata ttcattacta
     4741 tagtccatat tcatacaacc cttcattttg aatataatca attactttat ctggaacaag
     4801 gtagcggata gaacagcctt ctttgatctt ttgacgtatt ttcgtggaac tgatgtcgat
     4861 ctcaggtaca tctacccaaa ttacaggata tggagtatcc gttgtgtatc ctggtcgacg
     4921 tatacccaca aagttgacca tagaagtcag ctcatcgatt ttataccatt ttggcaaata
     4981 ttctaccata tccccaccga taataaagta ataatctgta tccggattgt tttgcgtcaa
     5041 ttccttcata gtatcatagg tgtaactttt cccaccacga gctaattcaa tcgtttcaat
     5101 ctgcaaaaaa ggattgtctt ccaccgctaa ttccaacatg ttgagtcgat gttttgcatc
     5161 aatcgtctgt ttctcatcaa catgaggtgg ctgataagaa ggcatcaaaa atacgcggtc
     5221 aagacctaaa ttccggccag cttgttcagc catcactaaa tgtgcaaggt gaacgggatt
     5281 gaatgttccg cccaagatcc ctacttgctt tttcttctct aagaattgag gcatttcttc
     5341 aggaaataat tgtgaacgaa caaatgtctt agcttgcgta ttcatcatgg tcactcctta
     5401 tattgctttt acttcttttg atagcctttg atacttttct tttgtggaag gtttgtataa
     5461 tacaataaca cgtccaatga tttggacgat ttcgcaatct atttcttttt ctaatacttg
     5521 agctgcttct tctgcaatct catctgtatt ttgaagtaac gatactttga tcagttctct
     5581 tttttctaaa gcttcttcaa tttgaacgat cattgctgaa ttgatcccac cttttccgat
     5641 ttgaaaaatc ggctgaagat gatgcgcttt gctacgtaaa aaacgctttt gttttcctct
     5701 taaactcata attttccttc ttccttaaat caatgctttg cgtttcaata catctacacc
     5761 ctttggcgcc cagcccgcaa ttactactgg cgctgttaca gtgatccagc ctagaccggc
     5821 aaaaacgatg tctgtttttt ctttcacaga aaactcaaaa cgaacaagct ccggaaatgc
     5881 ctcgacttca tcaggacgag gaggctgtaa taatccacct acatgttttt gataaaattc
     5941 gtctgctttt tccagtttcg ttcgatgaat atttacatca tttgacacat aagcaataaa
     6001 tgaagcacga ttgccgctga taaaatcaaa tcttgcaagt ccgcccaaga aaagtgtttg
     6061 cccttcattc agctgataaa ctttaggttt gatttctttt aacggtgcaa tgatccgcaa
     6121 atctttttta cctaagtagt gcgccatttg atggcgatgg ataattcccg gtgtatcaat
     6181 caaaaaatgc ccgtcatcta aaggaatctc gattttatcc aaggtcgttc ctggaaactg
     6241 cgaagttgtg attacatctt gtactcccgc tgtatttttg atgatttgat tgataagcgt
     6301 cgattttccg acatttgtta ctccgacgat atagacatcc cgtccatcgc ggtattcttc
     6361 gattttttct aatagttccg tcatctcatg agatttctta gcactagtta ataatacatc
     6421 tactggacga attcctgctt cgtaagcacg ttccttcatc cattgtgtta atttagtttt
     6481 tttcaatgat ttaggtaata tatccacttt gttcccaact aaaagtactg gattgtcacc
     6541 gacaaaacgg tgtagtcctg ggattagtga accattgaag tcaaaaatat ccaccacatt
     6601 gacgatcaat gcatcttttt gccctaattc attcagtaag cgcaaaaagt cgtcatccgt
     6661 caatgagaca tcttgggtct cattataatg gcgtaatctg aaacaacgct gacaatagac
     6721 ttccccggaa gctaaacctt tttccaaagc cgtttgcggc gtgtatccta attcaccggg
     6781 tttgtttgtt tgtataacgg caccacagcc gatacattgt aattcttctg tcattcgatt
     6841 ccacctcgcc agcccatatc agggtgtttt gctaatagat aggccataat tcttttttcc
     6901 caaaaacgat taaattgtgt cttccaacta tctgttgcga taatgggttt tacaaggatg
     6961 ctacgtatcc ctgcacgatt ggctccacgt atatcagtca taatctgatc gcctaccatt
     7021 actatctcat catctgctaa atccaattgt ttctttgcta tgttgatccc tcttgctaaa
     7081 ggtttcattg ctcgtgcaac atacaaaaga tcaaattttt caatcgctcg cgcgacacgt
     7141 tttgaattat tgtttgaaac aacaacaaca gggattcctg catttttcat ttctaaaatc
     7201 caagtaagca actcttctgt tccatctgga ttattccatg caatcaacgt attgtctaaa
     7261 tcggtcaata ctgctttgat gcctagattc tttaattggg caggtgtaat ctgatagatg
     7321 gcttctacca tccatgttgg tttgtaattt gaaaacatgc tgtctcctca tctgcaaaaa
     7381 atgaaggagc acataaagta ctcctcaacc ccgtattata ctgcatatca gcaaaaaaaa
     7441 ctacctctat actcgttcgt ctttcagaaa aagcatttct ttacaaaatg accaaaaata
     7501 aagattgatc acagaaaaat ttagaaacaa aaaacaacta atattggcgg aacctcttgt
     7561 atcgtttttc cagtaattta tgctgatcta atcgaacaag ttccgtcaat ctttcaatca
     7621 acgctttttt cataatgtgt gcgactttat atcgtggcaa ctgctcgccg ttgaacgttt
     7681 caggaataac tcgatcaacg attcctaact cgcgtagttc tggcgcagta attttcatca
     7741 actcagctgc ttctgcggcg cgactaccgt ctttccacaa aatcgacgca aatccttctg
     7801 gagaaagcac agcataaatc gtttcttcca gcatccagac ttcatccgct aaacctagag
     7861 caagggcacc gccacttcca ccttcaccaa tgatgatggc gatgatcggt acctttaaat
     7921 cagacatttc gagcagattt ctggcaatag cctctccttc gccgcgctct tctgctccta
     7981 caccgcaata tgcacctgct gtattgatca aggtaatcac cggtctattg aacttttccg
     8041 cttgtttcat cagtcgtaaa gcttttcgat agccttcagg atggggagaa ccaaaatttc
     8101 tgcggatatt ttcctgcaga tcccgtcctt tttgaatacc gatcactgtt accggtttgc
     8161 catctaatcg agcgattcca ccaacaatcg cttcgtcatc tgcaaataaa cgatcaccat
     8221 ggcattcctc gaaagaatcg aagatctctt caaaaaaatc caagcttgtc catcgttctt
     8281 tagctcttgc taactgaacg acatcatgtg ctgtcttatc cacgatctat ccatcctttc
     8341 tgtgtatgca attcaagaag cgtatggatt ttctgtttca attccatgcg gggaacaatc
     8401 tgatcgacaa aaccatgaga taataagaat tcagcctttt ggaaatcttc tggaagttcc
     8461 tgcttgatcg tctgttcgat gacgcgtctt ccagcaaaac cgatcaagct ttggggttct
     8521 gctagaataa tatcgccttc cattgcaaaa ctggcagtta ctcctccggt cgtcgggtcc
     8581 gttaaaacag ttagataaaa aagaccttca ttgctatgcc gtttgacagc agcagagatt
     8641 tttgccatct gcattagaga aaagattcct tcttgcatcc gtgcgccacc tgaagctgtg
     8701 aacaagacga ctggaagact ttcctttgtt gctcgttcga ataatctggt gatcttttct
     8761 ccgacaatcg ttcccatact tcccatgata aaattaggat ccatcactcc aataatgaag
     8821 ggaatcccct gaatggttgc tttaccagtg aggactgctt catgaagtcc tgttttatct
     8881 tgcatttttt taattttttc cgtatattcg ggaaatgcta gcggatcttt ggttacaaga
     8941 tccgtatccc attcttcaaa acttttttca tcaataatca aagctagacg ctgccatgca
     9001 ccgattcgaa aagcgtatcc gcaatgaggg catacttttt cttcaccgat gtctttcgta
     9061 taaaggatat gtttgcaatt tggacacttt gcccacatgt tatcaggaac agatggcgag
     9121 ttagcaggtt tgctctctct attaggattg atccgaatat atttcttttt cttaaacaga
     9181 gccatctatg cgcctcctat ctccgtttct ggtgtccaat tcggtaaaaa ttcttcttgt
     9241 aaaaatgctg tgctgtaatc acctgctatg actgcaggat gactaatcaa atccatctga
     9301 aattctgcgt tcgtgatgat tccttctgtg ataaattcac ttaaggctcg ctgcatcttc
     9361 attaatgctt caaaacgcgt atttccatgg acgattattt ttgcaatcat tgaatcataa
     9421 tatggcggga tcgtatatcc cgggtaaact gcactgtcta ctcgcagtcc catcccacct
     9481 gctggaagca gtaagttttg gatcttccct ggagaaggag caaaatggaa agcaggattt
     9541 tctgcattaa tccggcattc gattgcgtgt ccttggaata ctacttcttc ttgacacaca
     9601 ttcaaaggct caccggctgc tatctccacc tgttttttta ctaaatcgat tcctgtgacc
     9661 atttctgtta ctggatgttc cacttggatt cgagtattca tttccatgaa ataaaaatcg
     9721 ccttcttcat ccatcagaaa ctcgatcgtt ccggcatttt cataattgac tgctttagct
     9781 gctcgaacag cagcgtctcc tagtgcttct ctttttgttt gtgagatcac tacagaagga
     9841 gattcttcca gtactttttg attgttgcgt tgtaaagaac aatctcgctc gcctaaatga
     9901 atgacatgac caaatcgatc acctaagatc tgaacttcta tatggcgagc aggataaatg
     9961 attttttcca aatacatatc atcatttcca aatgccgctg ctgcttcttg ctgagctgaa
    10021 gaaaaatgct gtgggagctc ttcttttgat tgaactttcc gaatgccttt cccgccacca
    10081 cctgcagctg ctttcagcat cactggataa ccaattctgt ctgctatttc caatgcttct
    10141 tctactgtag ataatacacc agtacttcct ggaatgactg ggacatttgc ttcttgcatc
    10201 aattgacgcg cattgatctt atttcccatt gcatcgatcg ttgcactttt aggaccaata
    10261 aatgtgatat tgcactcttc acacatggag gcaaatcggc tattttcaga taaaaaacca
    10321 aatcctggat gaattgcttc ggcttttgtc acgattgcag cgctcagtac ttcctgaaca
    10381 ttcagatatg aatcaacggc ttttgccggg ccaatgcaga ttgcttcatc tgctaactgt
    10441 gtatgaagtg cttcggcatc tgcttctgaa tatatcgcta cggtttggat ccctagttcg
    10501 cggcatgcac gaatgatacg tacagcgatt tcaccgcggt tagctatcaa tactttagaa
    10561 aacatatttc acctatccaa tcataaaggt taattccgct tcggctactt ttttaccgtc
    10621 tactttagcg attcctttgc ctattccggc tgaagctttc actttcaata tctcaacttc
    10681 aaggtataag gtatctcccg gtgtaacttt cttacggaat tttgctttgt ccaatccgcc
    10741 gaaataagcc gtctttccct tgaattgttc aagagaaagt aatgcaactg ctcctgcttg
    10801 agccattgct tcaacaatca atacccctgg cataacaggt tcttctggaa aatgtccttg
    10861 aaaaaagggc tcattgatcg ttacattttt cttagcaacg atccgttctc cttcaaccat
    10921 ttcttccact cgatcaatca aaagcattgg ataacgatgt ggaatgattt cttttatttc
    10981 ttgtatgttc attgttttcc ctctttcctg ctttgcttat ttttgctgaa tcctaaagag
    11041 tggctgtcca tattcgacaa ctgactcgtt ttctacaagg atttctgtga tcactccgtc
    11101 ggcctcacta gttatctcat tcatcagttt catcgcttca ataatgcaga taacttctcc
    11161 tttggcaact tgatcaccta cttttttgaa agctggtttg tcgggcgctg gttgcagata
    11221 aacgattccc acgattggcg aaacaacctc gattccatca aatactgccg attctttttc
    11281 tttcattatt tctggagcag ttgttgcttg ttcaggtatg acattgactg gctccgaaac
    11341 tggcaacgtt tcgtttgttt gaaccatatt catgcctcga cttgttttat ttttattcat
    11401 atacaattca aattgacctt cacgcaaatc gaattccgtt agcgaggatt gatcaaattg
    11461 actgactaat tctctgattt cattgatatc caaaattatt cctcccagcg tttcatgcac
    11521 agaaccgcat tatgtccgcc aaaccctaat gaattactca ttgcataagt aaaacttgca
    11581 ggtttgcttt cgtttttgac aactgtcaag tcaatctcag gatcttgttt ttctacattg
    11641 atcgtaggtg ggataaattg atgttctaat gccagcaaag ttgccaaggc ttcgatcccg
    11701 ccggctgctc ccaataaatg tccagtcatc gatttcgtgc tgctgacgta agtatctctg
    11761 aaattttcac ctaatgcgat ttggattgct tttgcttctg cctggtcatt ggctggtgta
    11821 cttgttccgt gagcattgat atatccgact tgttccggct gtacaccagc ttcttccatc
    11881 gcaagcttca ttgctttaga tgcaccagaa ccatctggag taggagctgt catatggtaa
    11941 gcgtcacagt ttgcaccgta tccaacgatt tctccataga ttttggcacc gcgagcttgt
    12001 gcatgttcta aagattcaag aactagaacg cctgctcctt cacccatcac aaaaccattc
    12061 cgattttcgt caaacggaat cgaaccacga ttaggatcag ttgattctga gagtgctgtt
    12121 aaagctgcaa atccagcaat ccccatctca caaatcgttg cttctgttcc tccagcaaga
    12181 atgacatcag aatagccatg tttgatgtta cggaatgctt ccccaattga attcgttccg
    12241 ctagcacaag ctgttacgat agctgtacaa ataccttttg cacctacacg caaagcaata
    12301 tttccagctg ccatgttggc aatcgccatt ggtacgaaca ttggagaaac tcgttttgca
    12361 cctttttcat gtagacgaat aacttggttt tcgattgttg gaagaccacc gatcccagat
    12421 ccgatgatga cgccaaaccg gtcttgattc attttttctt tatctaaacc gctcatctcc
    12481 attgcttcta aagcagcgta gattccatag atcgaaaaca tatccattcg tttgctatct
    12541 tttttaataa aatatttatc gtaagggaaa tctttgactt cacccgctaa ggtaatccct
    12601 gtttctgaag catcaaattt agtgatcggt gcgatgccat tcgtcccctc ttttaagcta
    12661 ttcaaaaagc tgtctgcgtc atttccaata ggtgaagtga ctccatagcc agtaattacc
    12721 actcgattca tttgtttgct ctccttttct tatccgtgca tgaccaatcc gccatcgaca
    12781 ttgatgactt gtccggtgat atatggattt tgtgctaaga aaactgctgt ttgtgcaata
    12841 tcttctactt gtccgaaacg ctgcatcgga atttgctttt ccgcttgttc cttgactttg
    12901 tctgctaaaa cttccgtcat atcagttgtg atgaatcctg gcgcaatggc attacatgta
    12961 atgcctctag ttgcagcttc tcgtgccaca gattttgtta atcctactac gccagcttta
    13021 ctagctgcat aatttgcttg accaatattt ccaatcaacc cagatacact agagagatta
    13081 atgatggccc cttctcgctg cttcatcatt tttttcaata cgtgctgcgt catattgaag
    13141 gttccaatca aattgatatc cagacatttt ttgaaatctt ctgcatccat tcgcatcaca
    13201 agtttgtcat ttgtgattcc ggcattgttt actaacacat ggatcgatcc aagcttttct
    13261 tctgcttctt tgatcatttg tcccgctttc tcataatcag agatgtcacc ggaaactcct
    13321 acgcatttta caccgaatgc ttcgatttct tctatctttt cttttggaat ctctccgcga
    13381 ccattcaaaa tgatatttgc tccagctttt gcaaaagcta aggccatcgc tttgccgatt
    13441 cctcttgtac tacctgtgat aaatacattt tttcctttta catccataac gttcctcctt
    13501 atagtattgc tattgtttca tcaaatgtct gcttattctc aacacgcaga actggtattg
    13561 ttttatcgat ttttttcata aatccgctta atacttttcc ggggccgact tcgatgactt
    13621 gttcgatgcc gattgttttt aacttatgga tactctcata gaaacgaaca ggttgcatga
    13681 cttgctgttc aagtagtccc gcgattgttt ctttcttcat tatttccgtt gttgtgttgc
    13741 ttatgatagg aaaagaaggt tcagagaatt gaatctgact taggtccttc gccagttttt
    13801 ttgctgccgg ttctaaaata gctgtatgaa aaggcccgct gacatttaga gggatcattc
    13861 ttttgaaccc tttttctttt aatagtaaaa cagcttcgtc tactgctttt tcttcgccac
    13921 caatcacgat ctgctgaggt gtattataat ttgcaggaga tacgatgccg tacttgctag
    13981 cttcatgaca gctttcctcg atcgtttcaa tcggcgcatt catcactgca accattttcc
    14041 cgcttccagc tggggcagct tctgtcatat aagcgcctct ttttgctact aacgcaactg
    14101 cttcagtgaa ggatagcgca ttactagcta ctaatgccga atattctcct aaactcagtc
    14161 ctaaagctgc atctgggatg attccatgct ccttcaataa gcgataatac gcgatgctga
    14221 ctgttagaat agctggctgt gtatattgcg tctgatccaa acgttcattt tctgtaaagc
    14281 aaagttcagc catatcgtag ccgagaatct cacttgcttc atcaaatgtc tgctttacaa
    14341 cggcttcttc atataaatct tttcccatac cttgatattg tgcaccttgg ccactaaata
    14401 agaatgctgt tttcatgttt ttctccaata tatccagaat atatgtttga ttcttcctat
    14461 tcaaaaaggc cgagacattc ctatttcagg aaatgtccca gcccccaacc taattttaat
    14521 gattatgcag tttgtttttc tacgtaagtc actaaatctt gaaccgtttt gattccgtct
    14581 tctgtttcga ttttcacgtc aaattcatct tcaatttcat tgatgatttg gaataaatcc
    14641 aagctgtcag cttctaagtc ttcttggatg tcagtagtca atttgatttc ttcagtttct
    14701 tttcctaatt cttctgcgat gatttcttga attttttcga ataccataat aatatattcc
    14761 tccgatattg tttgcttttt atttttataa tttaacagtc tacaactcca tgagaatact
    14821 tccccatgtt aaacctccgc catagcctgt aaatattact ttctgctgtt ttcctaaggt
    14881 gatctttcca ctagaaactg cttcatccaa taagataggg atgcttgcag cagatgtatt
    14941 cccatattta tccatattgg tcaggaactt ttcttgcggc atctttactt ttcgggcaat
    15001 tttctcaata attctgaggt ttgcctgatg caaaagcaga taatcaactg gtttgtcttt
    15061 catgacttct tggatattgt ttgcaacgtc tcgtacggca aaatcaaaaa tatctctgcc
    15121 agtcatttgc aaataatacg aaccttctga atcttctgaa tagaacggat ttttattttg
    15181 ctggtagcca gcggtcaatg agctacttct ggtaccgtca gctgctaatt tttctttcag
    15241 caattttttc tgtggattcg cttctaagag aatcccacct gctccatcgc caaacaacac
    15301 agcagtcgta cgatcagacc aatcgattag cttagatagt gtttcaccac caatcaccag
    15361 tcccttagtt gagccgcagc ggatcatttt ttcagccatg cttaacgcat aaacaaatcc
    15421 tgagcacgca gcactgatat caaaagcgat agcatgagtt gctcccaatt tcccttgtac
    15481 ttgtgctgct acagatggtg tcgtgtaatc gggcgacatt gttgctacta aaataaaatc
    15541 caaatcttta gggtttagat ttttctgttt tatcaatttt tccgccacta aaatgcataa
    15601 atcagaagtc tcctgatttg ttacacatct tcgttcacga atacccgtcc ttgacgaaat
    15661 ccattcatca gaggtatcca tcatcttgga aagatcatta ttagtaataa tcttttcagg
    15721 aaggacgctg gctgttgcag taatcattcc aaattgttcc attaaatatc ctcacttata
    15781 gtcttgcaag aagtcgtgca agtttttcag tgcatccagc aaggcatgct gttcctccgt
    15841 agccattccg tctaagatgt tcttgaccat ttcacgatga aaatgctgat ggacccgaaa
    15901 tagtaacttt ccttttttcg ttagtcccaa ctttacaacg cggcggtcat cttcactgcg
    15961 gatacgttcg acatagcctt tttttactaa cttgttaata gcagtggtca aggtcccaac
    16021 agtaattgat agttctttgg ctacttctga agttgttttt ttcttgtaca tcccgattgc
    16081 ctcgatcgtg tgcatctctg tgattgacag atctttgaat tgagattttt tcagctcaga
    16141 ctcttcgatt gtcaaaatat cattgaagac actaacaaga taatcattta ctgtttttaa
    16201 atctggttcc atgttccacc gtccactaat tagtttgatt atcaaatctt ttgatgttca
    16261 aactatatct taactttttt caaaatgcaa ccttaaaaga tcattttttt actatatgag
    16321 ctttttttaa gattttactc atttaaaaca actgataaat cagcatttat aatttctttt
    16381 attagaaaaa tcatctatct cacatatttt gattatcaaa gtattttctg tgaacagaac
    16441 tatagaattc cctaaaactt gtccattatt tcaaaatttg ttatagtctt tttattcaaa
    16501 gcgtaaaagg agatcctatg ttaaaagaaa aaatgaaaac tctccccatg ctgccgggtg
    16561 tctatcttat gaaagaccga gatggtaagg tcatctatgt tggtaaagca aaacggctca
    16621 aaaatagagt aagcagctat tttcatcaga ataaacagca ttcaaaaaaa gtattacgta
    16681 tgattcatca tataacagat tttgatttcg tcgtagtaga cacggaatta gatgctttat
    16741 tgctcgagtg tcaattgatc caacattacc gtccttttta taaccgacaa atgaattatt
    16801 tcagtaatta taattatgta catattacca ataagggatt tgtattaaca gatactccta
    16861 cggctcgaac atacgggcct tttcgtctct ataaaaaaat gccgtctatt ttgcgtataa
    16921 tggaagaaac ttatcaaatg ccttggttgt ctgaaattag tttgttagct ttgcgagtcc
    16981 agcttcctga cctacaggaa atgacttttg aacagaaaaa aaaggaactc caagggctgt
    17041 ttcaaggccg aaataaaaag ctactgactt acttgaaaaa aagacaacaa catttcatct
    17101 atcagttgaa tttcgaaaaa gccggtatgc ttcaaaaaga tatcgaatta gttacttatt
    17161 ttattagacg gatccaggaa caaaagcagt ttttaagaac accatcgctc accttcagca
    17221 tgccattagc agcagatgag agtcagaaaa agcactacct cgtttgttat ggtcaacttg
    17281 cagaaacaat gattacctct ggtgacgttt ctcctgactt ctattatgaa aagaaagaag
    17341 ctcatttatc tttgaaacgt caattgtcaa aagaagaaat cgatcctgta caaatcttga
    17401 tcagttaccg caaaaaatta gaaaaagaac agtttgaaat tgaactgtta aacaaaaaag
    17461 aagctgaaaa gcaactaaat tagttgcttc tcggcttctt ttaggcatct tcaacttcaa
    17521 atgtcaatta tcctaaaatt tattggatta agtcatagat ttccatcgct acgatatcga
    17581 tattatcaaa ttcgtattgc tgatgagtat ctgtttcagt taattcaaat gtatcggtcg
    17641 ctttatcata agttactgta cacttctcta cgccttcttt ttcaaaacgg cgaacttgca
    17701 gttcattatc tgctgactcg accatagcct ccagtcgttt tatgattgcg actaattgtg
    17761 aatgtgtcat cggaggtcct ccttttctta tttctgttat attgtatcaa aagcatagcc
    17821 tacttgcatt gattttctct aaaaaaactc gttttttatg attttttttc tggtattttt
    17881 ccttcaggcc accaaagttt gatcaaagct tgggttccgt cattctcaaa acctttatct
    17941 gccaactctt catatagttt caatgcttgg gtagttgctg gcaatacaag atccattttt
    18001 tctgcttctt ctaatgcaat ctttaaatct ttgataaaat gtttgacaaa aaaaccaggt
    18061 gtgtaatctt cttttaaaat acgaggagaa taattgctta aagaccagtt agctgcactt
    18121 ccacctgaaa gcgtctcaat cactttcttt aaatcaagtc cgtttttttg tgcatagaca
    18181 agcatttccg tcattcctgt catcgtacct gcaatcatga tctgattagc catttttgta
    18241 tgctgacctt tcccagcaga accatgcaat gtaaatgtcg taccaaattc tttgaataaa
    18301 ggcagtactt gatcataaac ggcttcttct ccaccgacca tgatcgtcag tgtaccattc
    18361 tttgctccta agtcaccacc ggataccggt gcatctaacg catctgcgcc tttttctttt
    18421 gctgtttttg caattttttc tgccaggctt ggtgtacttg tagtcaaatc aactaaaata
    18481 tgtcctgaga tatctgcaga aaaaatcccc gactgtccat agtagatttc ttctacatct
    18541 ttggggtaac ctaccattgt aaagagaata ttactttgtt ctgctaccgc ttttggtgta
    18601 tctgcccaga tagctccttc tgttactaaa ggatcagctt ttgcttttgt ccgattatag
    18661 acagctactt cgtgtcctgc ttctagtaga tggcgtgcga cagcacttcc catgacgcct
    18721 gttccgataa aaccaagttt cattttttcg acctcctaaa aatcaaaaat ccttgcttct
    18781 atgctagtat acgctagaaa caaggatttt ttcataaaaa atgtttgaat tttatctttt
    18841 aaatgaattt aattttattt tgattcgttc aatcgagtct tcttcttctt ttcttcgcat
    18901 ctcttcttca tgataaacaa ccatgtcttg ataaaattcg atcatagctt cgccgaaatg
    18961 tgtagatgaa atatcataaa gtttagcttc taaaactttt ggatctttag gaacttcact
    19021 ggaaatataa tcaataagtg ctggtgcaaa gtcttcgtcg gtttcaaaag ttttccctaa
    19081 tgaaggatga tcgaacaatc ggttcaaata agcatttcct tccacgacac aaggtgttcc
    19141 ggctgccatc gcttctgtat aagtaagccc ttgtgtttct gaagtagaag cactgacaaa
    19201 ataatctgcc gctttataat aaagtgctac atcattgttg tccacttctc ctgtgaactg
    19261 cacaaattct tctaactgca gttctgagat caattcctct aactcttcta cataaggtcc
    19321 ttttcctacg ataactaatc tggcgttagg ataagcggag atgacctgag gaaacccatt
    19381 aatcaaggct tggatatttt tctcataaga aatcctgctt aaagaaagaa tcatcaaatg
    19441 gttttcctga agacctagtg actcccttaa tttgctgata tcctgctgag tgatatcaga
    19501 acgtttgaat ttttcaatgt cgataccagt aggaatcacg cgaagcggtg ctattacacc
    19561 atagctgcgt aatgtatcaa taacacgttc acttggacaa actacacctg tcgtatgatt
    19621 tgcaaataat ctagacaagt attttacatg ggattgcctg atcacttttc ctttaccgat
    19681 ataatgaagg tagtcttcat acatcgtatg gtaagtatgg atcagaggta ttttcaactt
    19741 cttagctacc atcttcccaa gtatccctgc tccgaattct gtatgggtat gaataagatc
    19801 tagttctagt tctttggcaa ccaaataagc ataccacatg cctctaacaa caactcttcg
    19861 atccttgaaa gaaataaatg gtacacttgg cattcgaatc acatcttttt ccaacttatc
    19921 cgcattagga tcggtcgttg taaaaatata gacttcatgc ccatgtcttt ctaattcttg
    19981 ttttaatgtt ttgatcgatg tagcaactcc gctgacttgc ggaaagtagg tatcggtaaa
    20041 aaagccgatt ttcatgtttt tcgcctccaa actagtcctt gacctgattt acttttttta
    20101 atttgctaga gactttttca ttcagtacac tttcgtatac atttttaagt tcttcaccga
    20161 ttttattgat acttcgctct tttgctgttt gataaccggc ttgtgtcgta ctcggtagtt
    20221 cttgattggt aatctgttgg atcagtcgga taaattcttc gtttgtcttc cccatatagc
    20281 agttcttgct atcttctaac cagccattat acactggaat atctcgaaca agtacgtttt
    20341 gtttgcttgc taaagcttct aaaacaacga tcccttctgt ctcttcataa gaagggaaaa
    20401 agaatagatc tgcacctgaa tacgcacctt ctatgatctc tccacgaatg taaccaggaa
    20461 acaggacatt ttcaggatga tcttctttta caattttccg gatgttccga ggaatcgagt
    20521 acatgggaac atgaccaaac caaataaatg tgtattcggg catcttctta gcaatttcta
    20581 cgaaatcaat gattccttta cgttcgaaaa acaaaccgac acaaatgatt actttttgat
    20641 ccggagacag tttgaaatat tctctgaact tttgttcttt cattggatca gggtgatatt
    20701 tctctaagtc gatcccattg gaaatagctt gaatcggcac acggatacca tagctttcca
    20761 acaactgttt tgaataaggt gttggcgtga tcagatagtc cgctttttgg tacaatccta
    20821 ccagatattt ttttacgata ggagaaagct ggttcgatct gataaaggaa tttcggaaat
    20881 cttcctctgt agaatgcgca tgatagacaa ctttttttcc ttctcttctg gctttattga
    20941 ccatattatg actattgatt ccgtatgtat tgatgtgcag gatgtcataa tcttcttttt
    21001 catctagtgt ataagagata ccgacttccg tcagagcacg tttttgatga tcaagcgcac
    21061 gcccgattcc agattttgca agcatctttt cactttcaaa atataataat accttcacct
    21121 tacgtccccc ttcacttata tagtataaca tagcctacaa actgtcactc aaacttaagc
    21181 cctatttttg tctattctta ctgacaaaaa cgttttctta aaaaagaaca aaaaaactgg
    21241 cgtagctctt ttacaagcca caccagcttt tgatacgttg tttattttac atattcatga
    21301 acaagttcaa cgacttcttc catcatatcg cattctttca atgcacgatc agcaagttca
    21361 gccattttcg ctgtgtccaa gcgtttcatt aaactacgcg ttttaagaat agatgtcgca
    21421 ctcattgaga actcatctag acccatacct actagcaatg gaacagctgt ttgatctcca
    21481 gccatttctc cacacatacc tgcccatttt ccttcagcgt gtgctgcatc aattacattt
    21541 ttgatcaaac gtaaaattga tgggttatat ggttggtaca agtaagaaac gcgttcgttc
    21601 atacggtctg ctgccattgt gtattgaatc aaatcgttcg tacctacaga gaagaaatct
    21661 acttcttttg caaacttgtc tgctaagact gctgctgctg gaatctcgat catgatgccg
    21721 acttggatcg tatcagatac ttccttgcct tcacttatca atttttgttt ttcttcttca
    21781 aagatcgctt ttgctgcgcg gaattctttg agtgtagcta ccatagggaa catgatacgt
    21841 aaattaccat gaacagatgc acgaagcaat gcacgcattt gtgtacggaa catttcgtcg
    21901 ccttgttcag ataaactgat acgcaatgca cgatatccta agaatggatt catttcatga
    21961 ggtaattgca gatatggtag ttctttatct ccaccgatgt ccattgtacg aacaactaca
    22021 ggttttcctt ccataccttc taatacagct ttgtaagctt catattggtc atcttctgtt
    22081 gggaagtctg gagaatccat atacaggaat tcagtacggt acagaccaac tgcttctcca
    22141 ccattattat ggacaccaac tagatctttt ggtgttccga tgtttgcagc taattcaaaa
    22201 tgcttgccgt cagctgtgac agtttctgca ttttttaatt tttcccattc tgctttttgt
    22261 gctgcgtaat ctgcacctgc tttttcaaat tcagcttttt gttcatctgt aggatcgatg
    22321 attacatcgc cttcgatccc gttgacagcc aaaatatcgc cttctttgac tttagcagta
    22381 atttcttttg tcccaacgat tgctggaatt tctaaagaac gtgccatgat agcagaatgt
    22441 gaagtacgtc caccaatgtc tgttacaaac gcttttacaa aattgcgatc taattgtgct
    22501 gtatcacttg gagtcaagtc atgtgcaaca acgaccactt cttcgttgat cattgatgga
    22561 tttggtaaag taacgcctaa taagtgcgct aaaatacgtt tagcaacgtc acgaatatct
    22621 gcagcacgct cttgcatata agcattatct tccattgctt cgaacatacc gatatacata
    22681 tccgttactt cttttagcgc tgattcagca tttacgctgt tgtctttaat attttgcttg
    22741 atttgaccta ccatttctgg atctgaaaga accatcaaat gggcatcaaa tacttgcgct
    22801 tctgcttcac ctaagctttg cgctgctttt tcacgaattt gctgcaattc ttcagtagat
    22861 ttagctaatg cagcatccaa acgagcttct tcagccgcag tatcttctac tgttgcctta
    22921 ctgaatgtta aatccggttg aactagcagg taagcttttg caacggctac accgtcacta
    22981 gcggcaatcc cttttagcat ttcaaccatt attcagataa gccttctttc ttcattgttt
    23041 caacgattgc tgccattgca tcagcttcgt cagcaccttc tgcagtaata gtaacatcag
    23101 aaccttgacc tacacctaaa gacataacgc ccatgattga tttcaagttt actgatttac
    23161 ctttatactc taagtttaca tcagagttaa atttgcttgc tgtttgtact aatagtgtag
    23221 ctggacgagc gtggatccca gtttctgcta ctacgtgaaa ttctttcttt tccatattaa
    23281 tcggtctcct ttaagtgagt gttgttttta tgtgagggtt ttctttttta gtaattcaga
    23341 gaatcctaac aaatgccacc ctttcatctg taaagattaa catttttttt cgttagatac
    23401 aacagttttc attcaatttc agataaaagt attcaaatta ttccggattc tttttcatcc
    23461 ttttcttcac attttataac aaacgtgttt atttcctttt tcttgtcgct ttctcttctc
    23521 ctgacgttta tcattctttc ctttgaccct ctttgttttc atatgaaacg aattacttgc
    23581 ttcccttttt atttttagta taataagagt caaaggtcag gaatggtcaa tagagatttc
    23641 accttttcta cctcataaat ctcttaaaaa actaggggtt attcttttac ctcatgaaaa
    23701 acgagatgta aaatatggat gtacccattt ttgtgaaagg acgtgaatga ttcatgcttt
    23761 gtcaaaactg cggcaagaat gaggcaacga ttcacttgta tgccactgtc aatggtcagc
    23821 ggacacaact cgattactgt cagagctgtt atcaaaaaat caaaaatcaa caaaatggag
    23881 gtctgactaa tatggcccaa aatgatccat ttggatttgg cagtttggac gatcttttcc
    23941 gttccatgtc tcgccaaatg caacagcaag gaaattatga gcaaacacca ccaacacaat
    24001 ttggcggaaa caacaacttc aatggtggtc agccacctca aggcaataca gaaggtttat
    24061 tgggagaata cggaatcaat atcacagaac aagcacgaca aggtgatatc gatcctgtga
    24121 tcggacgcga tgacgaaatc aaacgagtca ttgaaatttt aaatcgccga accaagaaca
    24181 atcctgtctt gattggagaa ccaggtgttg gtaaaacagc agttgttgaa ggtttagcac
    24241 aaaaaatcgt agatggtgat gtaccgcaaa aattaatgga caaagaagtc attcgtttag
    24301 atgtcgtatc tttagtccaa ggtactggta tacgaggaca atttgaagaa cgtatgcaaa
    24361 aattgattga agagatccgt caagcagaaa atgtgatcct attcatagat gaagttcacg
    24421 aaatcgtcgg tgctggttct gctggtgatg gaaatatgga tgctggtaat attttaaaac
    24481 cagctttagc tcgaggagaa ttgcaaatgg ttggtgctac taccttaaat gagtaccgca
    24541 tcatcgaaaa agacgctgct ttagaacgtc gtatgcaacc tgtacgggta gatgaaccaa
    24601 ctgtcgaaga aacgatcagt attttgaaag gtttgcaaaa acgttatgaa gactatcatc
    24661 atgtaaaata tactgatgag gccatcgaag cagccgcaac tttatccaat cgttacgtcc
    24721 aagatcgctt cttgccggat aaagccatcg atttgctaga tgaaacaggt tctaagaaaa
    24781 acttgacgat ccaaatcgtt gatcctaaaa caatcgagaa aaaactgcaa gaagcagaag
    24841 aacaaaaagt tttagcttct cgagaagaag atttcgaaaa agcagcttat tatcgcgatc
    24901 agatcaataa acttcaaaaa atgaaagaac gtcagctcac tgaagaggaa acacctgtga
    24961 ttaccgaaaa agatatggaa aaaatcgttg aacaacggac aggtattcca gtgggtgaat
    25021 taaaagaaaa agaacagaca caactgaaaa acttagcaga tgacttgaaa gctcatgtca
    25081 tcggacaaga taacgctgta gatcgagtag cgaaagcgat tcgccgaaac cgtgttggat
    25141 taaacaaaca gaatcgtcca atcggctcct tcttatttgt tggaccaact ggtgtaggta
    25201 aaacagagct tgcgaaacaa cttgcctacg aactattcgg ttcacaagat tcaatgattc
    25261 gtttcgatat gtctgaatat atggaaaaac atagtgtctc taaattgatt ggttctcctc
    25321 caggttatgt cggatacgaa gaagctggtc aattaacaga aaaagtacgg agaaatcctt
    25381 acagtcttgt cttgttagat gaagtagaaa aagctcatcc agatgttttg catatgttct
    25441 tgcaaatctt agatgatggt cgattgacag atgcacaagg aagaacggtc agctttaaag
    25501 atacgatcat cattatgaca agtaatgccg gaactggtaa agtagaagca aacgttggtt
    25561 ttggtgcggc acgcgaaggt gttactcgtt ctgtattgaa ccaattaaac aactacttta
    25621 caccagaatt tctgaaccgt tttgacggga tcatcgaatt cagtgcatta tcaaaagaaa
    25681 acttgatgac aatcgtcaca ctaatgctag acgatgtcaa ccaaatgctg gcagcacagc
    25741 aacttcacat cgaagttcct acgaatgtca aagaaaaatt agttgactta ggctatgatc
    25801 catctatggg tgctcgtcct cttcgacgca cgatccaaga acaaatcgaa gatggtatcg
    25861 ctgaatttta tcttgatcac ccttctatcc atgagttgaa agcaaaactg gataaagatg
    25921 gaaaaatcat cgttacttct aaaccagaac gtttggctaa agaatcagcc gaagaaacgg
    25981 ctgaataaat ccagttttat agcgtgagac ggtatttctt tgagaaatgc cgtctcattc
    26041 ttttttcaga aaagtcaaaa tactgttcat tttataaaaa agaaattata ataagagaaa
    26101 caagcaatta aaaatacata aaagaggtga gcgccatgaa attggtaaat gtaacaaaca
    26161 gccattcccg tcttgtatta aaccagcttg aaaacactga tgcccatcta gtcaaggtct
    26221 acacagccgg aaataccact attgtctaca ctgaagcccc tgaacataat gaaattctgc
    26281 tgatcaatga caaacgtaaa atccagccga aagaaatcga agatgctaaa aattatttct
    26341 tagataaaat caaagacgcc gtttaccacg aggatgaaat caaagtaatc gaattggcag
    26401 gccttgttga aatctcgatt ccaaaacatg aattaggcgc tacattagcc gtgtaaacaa
    26461 attttgaaca taataattaa agagcgtggg acaaaagtaa agaacacttt tgtctcacgc
    26521 tctaaacacg tataaacggc gaaagcagaa gcaactcctt cggaaataag ctgaaattca
    26581 cgaaaattgc ttttcaaatt tttgtgaatt ccagcttatt tcttggagtt aaacgcttct
    26641 gtctcagcct ctttttcttt ctagcttttt gatttttctc ttttcttttg tacttttctc
    26701 ttttcatttt cagttctacg cgtgtcttta tttttaactt tttcctcttt atggatgatt
    26761 gatcgtactt tttgtgcaat ttgctcgttt ttgtcccagt cttttacatc taataatcga
    26821 cataatcgta acatctcttc ttttttctgt ttttttcttt cacgtctttg ccattctgtt
    26881 tccatgtgtc acactctcct ttctttaatc atatgtacgc aaaataattt ttatattttt
    26941 ttgattagac atttctatga ttttaatata gtaaaaagag ccgtcacgga aaatccgtca
    27001 cggctctaaa caaaatatga gaggctgttt ttgtttggct attcttatta cttgatgcag
    27061 ggcagcttgt gtcaagactt ctttacatca aactttttaa ttttacattg ggatatttat
    27121 ccgcaaacca gcgttctgca aattggttct cgaataagaa caacggttgg tcgaaacggt
    27181 cacgagccaa gatattgcga cttgaactca tcttctcatc caaatcttct ggatcgatcc
    27241 agcgagcgat tttgtgtccc attggagaca tgattacttc ggcgttatac tcatttaaca
    27301 tacgatattg gaatacttcg aattgtagct gaccaacagc tccaatgata tattcttccg
    27361 ttagatatgt tttataaagc tgaatcgctc cttcttgaac taattgatag attcctttgt
    27421 ggaaggattt ttgtttcatg acattttttg cagtcacttt cataaacagc tctggagtaa
    27481 atgaagggag ttcttcataa gcaactttta gttttccttc gaacaatgta tccccaatct
    27541 gataattgcc tgtatcatat acaccgatga tatcgcctgc tactgctttt tcgacgtttt
    27601 ctctcgaatc tgccataaat tgtgtgacat tgcttaattt cagtttttta tttgtacgtt
    27661 ccaaccagac atccattcct cgttcgaatg ttccagaaca gattcggaca aaagcgattc
    27721 ggtcacgatg ggctggattc atatttgctt ggatcttgaa tacgaaacct gaaaattctt
    27781 cctcataagg acttaccatc tgatcatctt gtgttttgtg tccataagga gctggtgcca
    27841 attcaacgaa agtctctaaa aaagtctgta caccgaagtt agtcaatgca gaaccaaaaa
    27901 atactggtgt ctgatcgcca cgagcaattt tttcttgatt aaatgcatct ccagcctctt
    27961 tgattagttc tacttcttct aagacttgtt catataagga attttcagtt agaggatgat
    28021 tttcgggaag cagtccatct tcatccaatt ctactaatcg ttcatcctga taattttccg
    28081 gacgataaaa ctctacacga ttatggtaga tatcataaag accttctagc cctttaccca
    28141 taccgattgg ccaattcatc ggataagatt cgatatctaa tagctcttct aattcttcta
    28201 acaattccaa tggttcacga ccatcacgat ctagtttgtt gataaaagta aatatcggga
    28261 tgccacgttt ttttacaact tggaataatt tctttgtctg tgcttcgatc ccttttgcac
    28321 tatcgattac catgaccgca ctatccacgg ccatcaaagt acgataagta tcttcagaaa
    28381 aatcttcatg tcctggtgta tccaagatat ttactcgttt cccttgataa tcaaactgca
    28441 taacagaact agtaacagaa atcccacgtt gtttctcgat ttccatccag tctgatttgg
    28501 caaaattacc tgtttttttg ccttttacgg tcccggcttg acggatagcc ccaccaaaca
    28561 atagtaattg ttctgtaatc gttgttttcc cagcatccgg atgggaaatg atcgcaaaag
    28621 ttctgcgatt gtccacttgt tgctttaatt ttggattatt cataacctaa ctccatttct
    28681 tccattcaat tatttatatt ttctctaggc acagattgtc cgatctctta aagaaagaca
    28741 atttcatata gggctgtgac aaaagccgtg tagcagtctc tttttccgag taaacggcgt
    28801 tctagagaca gcctatcagc aataagccca cccaagccaa aaatacgaaa agctgttttt
    28861 gcttggctgc tcttattact taatgcagga tagctttttc acaacctttg tcaactacta
    28921 caactgaatc agtatagcat ggattcagcg aatgaaaaag ccaccaaacc ataagagtca
    28981 aatatttttt tgtcagctga ctaaaagaaa agacgtgttg cttgtttatt caagcaacac
    29041 gtctttcata accaagtttc caattatctt cttggctcgt catcttcgaa ttcttttctg
    29101 aagtatcttc gttcttcttc aggttccgct tctgtttctt ccggatcatg aaaaatgaca
    29161 cggattttaa ggattcgaga accttccatt tcttcagaaa ccagcgtaat attatcgaca
    29221 tcaaacgata acttttcgcc ttcatccgga atcatgccta atgctgtaat caaataacca
    29281 gccatcgtat ctacatcact catatgcaga tcactgtcaa aagcttcatt gaactcatca
    29341 atcagcatac gtccttggat gatatattcg tgttcatcga ttttttcata aagattctca
    29401 acttcatctg attcatcatc gatttctccg acgatttctt ctaaaaggtc ttctagtgtt
    29461 gctaacccaa cgacgccgcc atattcatct aataaaatag ccatctgatt ctgcgtcttt
    29521 ttcatttcat ataacagatc atcaataaag accgtttcag gaacgaataa aggctcctgc
    29581 atgattttct tgatatctaa attatcaaag ccgaatttat gggcagcttt tagtaaattc
    29641 tttgtatgaa ggattccgac tactttatct ttgtcttcgt tataaacagg gattcttgaa
    29701 tagttttcgg ataaaacttc attcacattt tcttccacag tgtcgttgat atcaatcata
    29761 aatgcatccg ttcgtgggac catgacttct cgggcaactt tcgtatcaag agaaaaaacc
    29821 ccttggagca tttctaattc ttcattattc aatacgcctt cgttttcaag catataacgc
    29881 atttcatctc tggtcatttt tgaatcttca tcatcaaaag tcatcggcgt gatacgcgcc
    29941 aataaacttg tggaagcaga aagcagccag acgaagggct tagctactac accaatcaag
    30001 cgtatcagcc ctgaagtgaa ctttgcgact tcttctgatt tatttaaagc aatacgttta
    30061 gggtacaatt ctccaaaaac aatcgataca taggtcaaaa tagccagcac aatgatattt
    30121 gcaatatttt ttgccgctgt gccgccacct aataccggag ctaaccgttc cgataaagta
    30181 ttagctagtg aagcaccaga caaaatgttg accaaagtga ttcctacttg gattgtcgat
    30241 aggaaattat ttggattctg caatattttt aaaagctttt gagatttcac atctccttca
    30301 tcagcttttt gctcgattcg attcttattg atcgaaacaa cagcaatttc ggaagctgct
    30361 aaaaaagcat tgatcaatgt caaaacgatc aataataaaa tctgcgctaa cagcgactga
    30421 ctctcagggt cagcattcat aatcgcattc tctccttata aaattaattt ttagtttacg
    30481 atagaaaaaa acgtggcact atagccacgt taaaatggta tcacaaagca attttctttg
    30541 caataaaaac aagtgtacta aaactaccta cgaggtcgta gcaattttct tcttgttttc
    30601 attttgcttg tcttctttga cgattttgat tatataggaa atgatcgtct tgcagattgc
    30661 gtaaaaagga acacctaaga agataccaag caatccggca atatttccag ctactagaag
    30721 aacgataatg atcgttaatg gatggatttt cagtgatttc ccaattacat taggataaat
    30781 gatattccca tccaactgtt ggacgattag cacaaccaca caacaaagta atgcttttac
    30841 cggatcattg aagactgtta ctagaaatgc aggtgctagt cctagataag gtcctaaata
    30901 tggaattagg tttgtcaatc ccgcaatgac cccaaataag aaagcataat ccacaccaat
    30961 taccatatat cctaaaaatg tgaaggtacc aacgaataaa cattcgattg cttgtccgct
    31021 aatgtaatta gccaatgttt tattcagttg ccccaataac tccacaattt ggtttcttct
    31081 tttttctggg aaaaactgtt ggatgtttgg cactagtttt tctccgtctt tcaacatata
    31141 gaacaagatg aatggtgctg taatgattac tacggttgcc gaagcgatcg ttccaacgat
    31201 cgaacctaaa ctattggata ctccactaag gaattgctga atgattgtcc cgtaagaaat
    31261 gtctaattgc tcaaaatatt tattgatatc tacttccttg aacaaaggca attcggctac
    31321 ctcttttaac cagctttcta cctgtttgat gaaatcaggc atattagatg ccaatgagga
    31381 aatttgctgt actagacttg ggatgacact aagtaaaatc caaataattg ctgcaactaa
    31441 aaggagcagc acaattgcga cagcccatat tcttttgatt tttgctttca tcaacagctt
    31501 gacaatagga ttcaataaat agtaaagaaa gcctgcaatc agtacaggtg caaataacgt
    31561 ggaaaaaaat gtcccgatcg gttcgaaaat aaagtttatt tctgatgaaa tcatgatcaa
    31621 tgcagctatg atcaataatt caagcgacca aaacattagt ttagactgtc taagtttttc
    31681 aaacaataga tttcctcctt tctttcttac tagtatatat tatattcgtt aaaaattctt
    31741 ttattctttt tttatttttg aaaacttgcc atacttttta tttgttttat tttccagtat
    31801 actatacgaa aggaaggtgg ctagaccatg aaagttgtac aaacaaaaga tacaatgagc
    31861 aatatttact tagatgctgt acggatcagg catcaagttt ttgtagtcga acagggtgtg
    31921 cctttgtctc gagaaataga caaagatgaa gcccattgta tccacttcgt cctgtactca
    31981 gataaaaaag aaccgcaagg aactgtacgt cttttgcctt tagaaaacgg aaaaatgaaa
    32041 ttacagcgga tggcgattct ttctgaatac agacaccaag ggttaggaaa aatattgatt
    32101 gaagaagcag aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag
    32161 tccacagctg aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa
    32221 gatgctggca tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa
    32281 gcaaaaaaca gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt
    32341 tacttacgcg acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac
    32401 gggccatctc cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag
    32461 tcaatatcgt tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat
    32521 agtcgtttct cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag
    32581 gcctcatcgt ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct
    32641 ctgatcattg ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg
    32701 cgcgttaccc atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc
    32761 atagagtgtt cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg
    32821 tcgtgcttgt aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc
    32881 cttccttaag cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt
    32941 attatatcaa taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct
    33001 aactgatcta ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc
    33061 ttcaaccact tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt
    33121 ccgtaaaaaa ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa
    33181 tgtcgtgtct gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct
    33241 gctactaggt catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc
    33301 tcatactcct cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt
    33361 tggctatctc tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata
    33421 taataaacgt cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct
    33481 cgtttgttag ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt
    33541 gtacagatag ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca
    33601 tcaatgattt tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct
    33661 gcttcaatgc aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct
    33721 ggaagtccaa atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat
    33781 gagctaattt cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta
    33841 gcagcagctt ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa
    33901 agcttttctt ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga
    33961 taaaaataat cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag
    34021 atacgataag gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat
    34081 tgaatgaact gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta
    34141 ttcactttcc tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt
    34201 ttgatataag ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct
    34261 tcgccatagt ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc
    34321 ttgcggaaga tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa
    34381 tgtttgttgg atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc
    34441 tttttcggct gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc
    34501 ttgttcaatg atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg
    34561 tcctttgtca cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc
    34621 gacaaggtcc aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc
    34681 cttttctttt aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt
    34741 cattgctagg tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc
    34801 ttcgtctaat tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg
    34861 taaagcttct ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct
    34921 cgtaacggtt aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat
    34981 ccccgtaatc aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact
    35041 tatggcagaa acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg
    35101 cataaatggt gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact
    35161 accaaaagaa gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga
    35221 attaataaaa gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta
    35281 tgaatgcggt ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa
    35341 ggatctgctt ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact
    35401 tttgccgacg atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt
    35461 acgaaaaaat aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca
    35521 gttagtccct ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac
    35581 tgttgttcaa ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg
    35641 aatcttccgc acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt
    35701 tattactcgc agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg
    35761 actgcccgaa gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat
    35821 gggggatgaa acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt
    35881 gactttcgat ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct
    35941 ttatggcgaa ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta
    36001 ttgcggagtt gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat
    36061 ggataccgtt cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca
    36121 caacactcga tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg
    36181 ttttcagcct gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat
    36241 ccaatcatta ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac
    36301 acttgccaga gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt
    36361 cgacatgttt cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga
    36421 ctaaatgaaa gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca
    36481 aaaatagctc ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac
    36541 actatttatt taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta
    36601 ttggatattc acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac
    36661 tgctaaaatc acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt
    36721 ttttggtgga acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt
    36781 caaaggttga ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg
    36841 atgttgtgct gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg
    36901 ccatctgtta gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat
    36961 ttttccatca atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct
    37021 tccgacattt tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg
    37081 agaagtaata taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac
    37141 taacccaata aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa
    37201 ccacattgta aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg
    37261 aagtaaaaaa ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac
    37321 aaatagaaaa catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac
    37381 aaatgttcaa ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat
    37441 acaggtcttg ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg
    37501 aagatgataa aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt
    37561 ttatcagggt tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca
    37621 tttgcaccac ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt
    37681 atttctcttt atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt
    37741 ctttttgtga ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga
    37801 aatattttca ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg
    37861 tatcagttgt ggctgcatca tcgcctggca atgtggctca tgattcctta ttatttatgg
    37921 gtactattcg caactctatt gaattattca atttattttt taaactaagc gctaaaaaga
    37981 gacgtcctgt aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt
    38041 taggtggcac tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac
    38101 tcattattct ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt
    38161 tgagtatctt gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta
    38221 ttcggatctt ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct
    38281 tcactattcg atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc
    38341 attgaagcat tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt
    38401 ttcttcagtt cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata
    38461 tcattgatca tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg
    38521 gtgtagttcg tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc
    38581 tcaactgctt ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg
    38641 acgattttcg tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct
    38701 tcgtgtcgca ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact
    38761 ggtacatgga caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc
    38821 gcattgatca aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata
    38881 tcgagcagtt gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga
    38941 tttaatttat ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg
    39001 atgatcgttg attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt
    39061 ccttgttccg ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt
    39121 tctttgtcat caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta
    39181 gaaattttgt ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat
    39241 ccggcaatca gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc
    39301 ataatacacc tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca
    39361 aattcacttt ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt
    39421 tttaaaattt ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg
    39481 aatacacgca gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa
    39541 caacaaggta ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt
    39601 tttaacagtc tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat
    39661 gactggtcct tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct
    39721 cttaaaatga ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc
    39781 ctcttcaaag taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt
    39841 atactagtgg tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa
    39901 atccattact aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga
    39961 acaaatgcat ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga
    40021 ctctctaaga agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct
    40081 tgaggtccgc gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc
    40141 ttgaagccgt ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact
    40201 gtaataaacc caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat
    40261 gtatatcctc caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga
    40321 aaacgctagc aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg
    40381 aggaaatggt tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt
    40441 ccagaatgct gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc
    40501 ccaaacgtcc aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc
    40561 agtagaggta atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa
    40621 tcgtccagcg gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc
    40681 attaatcgct tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc
    40741 ccagaaaaat gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc
    40801 tgttggaata gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg
    40861 gtatttctca attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg
    40921 cagatgcatc gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc
    40981 tggtaatgtg taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt
    41041 acaaagagta acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact
    41101 ttgacctact tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat
    41161 atagttcgca aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt
    41221 cataacattg atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta
    41281 gaagaagaac ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa
    41341 tacccaaagt ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt
    41401 ggataaggcc atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac
    41461 aatcgtagcc aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc
    41521 taaatatcct tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct
    41581 tatttatcat actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt
    41641 tgatcctttg cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt
    41701 aataaagaaa ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa
    41761 tcaatatgtc cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga
    41821 acatccttta ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca
    41881 ggtgcacttt gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat
    41941 atatatatcg aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg
    42001 atcaaaattg ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa
    42061 taactagatt ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac
    42121 aaattcgcga gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct
    42181 ggtgaagtga accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg
    42241 aatgagttga tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta
    42301 tttgcttctt ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca
    42361 ctaaggttat ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata
    42421 aattctgata aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat
    42481 ctctgtggct gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag
    42541 acaagaataa catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg
    42601 tatttcactt gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt
    42661 atgacaacaa aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg
    42721 agaaatgtcc cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct
    42781 ttgccaatct cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag
    42841 attgctactc gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa
    42901 gaaggattca tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga
    42961 acagaacagg acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa
    43021 aacaaactta tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac
    43081 caattagaac ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt
    43141 tcttttttaa gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct
    43201 atttatccta gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt
    43261 ctctatgcac gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc
    43321 tattcaattt cattgcctac tataattgca acaatcttga tggtctttct tccttctttt
    43381 gacactagcg ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga
    43441 tgggcaaaaa tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga
    43501 cccttttaga ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc
    43561 tttctcaaaa aacaagcaag gcgcttcaaa gttcttcttg tatgc
//
LOCUS       DACCYH010000015        43488 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.015, whole genome shotgun sequence.
ACCESSION   DACCYH010000015 DACCYH010000000
VERSION     DACCYH010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.015"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            139..2736
                     /gene="adhE"
                     /locus_tag="IVY85_000772"
                     /gene_synonym="adhC"
     CDS             139..2736
                     /gene="adhE"
                     /locus_tag="IVY85_000772"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP8043806.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2965..4071
                     /locus_tag="IVY85_000773"
     CDS             2965..4071
                     /locus_tag="IVY85_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8043807.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4088..5116
                     /locus_tag="IVY85_000774"
     CDS             4088..5116
                     /locus_tag="IVY85_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP8043808.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5122..5601
                     /locus_tag="IVY85_000775"
     CDS             5122..5601
                     /locus_tag="IVY85_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP8043809.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5617..6912
                     /locus_tag="IVY85_000776"
     CDS             5617..6912
                     /locus_tag="IVY85_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP8043810.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6923..7747
                     /locus_tag="IVY85_000777"
     CDS             6923..7747
                     /locus_tag="IVY85_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8043811.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7912..8811
                     /locus_tag="IVY85_000778"
     CDS             7912..8811
                     /locus_tag="IVY85_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8043812.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8937..9806)
                     /locus_tag="IVY85_000779"
     CDS             complement(8937..9806)
                     /locus_tag="IVY85_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8043813.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9959..11419
                     /gene="rhaB"
                     /locus_tag="IVY85_000780"
     CDS             9959..11419
                     /gene="rhaB"
                     /locus_tag="IVY85_000780"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP8043814.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11416..12672
                     /gene="rhaA"
                     /locus_tag="IVY85_000781"
     CDS             11416..12672
                     /gene="rhaA"
                     /locus_tag="IVY85_000781"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP8043815.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12686..13531
                     /gene="rhaD"
                     /locus_tag="IVY85_000782"
     CDS             12686..13531
                     /gene="rhaD"
                     /locus_tag="IVY85_000782"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP8043816.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13533..13844
                     /gene="rhaM"
                     /locus_tag="IVY85_000783"
     CDS             13533..13844
                     /gene="rhaM"
                     /locus_tag="IVY85_000783"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP8043817.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13926..15077
                     /gene="fucO"
                     /locus_tag="IVY85_000784"
     CDS             13926..15077
                     /gene="fucO"
                     /locus_tag="IVY85_000784"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP8043818.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            15204..15962
                     /locus_tag="IVY85_000785"
     CDS             15204..15962
                     /locus_tag="IVY85_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP8043819.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            16062..16706
                     /locus_tag="IVY85_000786"
     CDS             16062..16706
                     /locus_tag="IVY85_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8043820.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            16713..17720
                     /locus_tag="IVY85_000787"
     CDS             16713..17720
                     /locus_tag="IVY85_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP8043821.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            17736..18578
                     /gene="kduI"
                     /locus_tag="IVY85_000788"
     CDS             17736..18578
                     /gene="kduI"
                     /locus_tag="IVY85_000788"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP8043822.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            18598..19401
                     /locus_tag="IVY85_000789"
     CDS             18598..19401
                     /locus_tag="IVY85_000789"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP8043823.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(19650..20714)
                     /locus_tag="IVY85_000790"
     CDS             complement(19650..20714)
                     /locus_tag="IVY85_000790"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP8043824.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(20711..21796)
                     /locus_tag="IVY85_000791"
     CDS             complement(20711..21796)
                     /locus_tag="IVY85_000791"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP8043825.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(21809..22786)
                     /gene="mvaD"
                     /locus_tag="IVY85_000792"
     CDS             complement(21809..22786)
                     /gene="mvaD"
                     /locus_tag="IVY85_000792"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP8043826.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(22779..23723)
                     /gene="mvk"
                     /locus_tag="IVY85_000793"
     CDS             complement(22779..23723)
                     /gene="mvk"
                     /locus_tag="IVY85_000793"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP8043827.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            24045..24698
                     /locus_tag="IVY85_000794"
     CDS             24045..24698
                     /locus_tag="IVY85_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8043828.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            24783..25688
                     /locus_tag="IVY85_000795"
     CDS             24783..25688
                     /locus_tag="IVY85_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8043829.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(25690..26322)
                     /locus_tag="IVY85_000796"
     CDS             complement(25690..26322)
                     /locus_tag="IVY85_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8043830.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            26642..27166
                     /gene="infC"
                     /locus_tag="IVY85_000797"
     CDS             26642..27166
                     /gene="infC"
                     /locus_tag="IVY85_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP8043831.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            27238..27438
                     /gene="rpmI"
                     /locus_tag="IVY85_000798"
     CDS             27238..27438
                     /gene="rpmI"
                     /locus_tag="IVY85_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP8043832.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            27491..27850
                     /gene="rplT"
                     /locus_tag="IVY85_000799"
     CDS             27491..27850
                     /gene="rplT"
                     /locus_tag="IVY85_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP8043833.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            28102..29439
                     /locus_tag="IVY85_000800"
     CDS             28102..29439
                     /locus_tag="IVY85_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP8043834.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            29487..31616
                     /locus_tag="IVY85_000801"
     CDS             29487..31616
                     /locus_tag="IVY85_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP8043835.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            31591..32280
                     /locus_tag="IVY85_000802"
     CDS             31591..32280
                     /locus_tag="IVY85_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043836.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(32688..33365)
                     /locus_tag="IVY85_000803"
     CDS             complement(32688..33365)
                     /locus_tag="IVY85_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8043837.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTRFRWLR
                     LTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFED
                     IQDRPIK"
     gene            complement(33501..33695)
                     /locus_tag="IVY85_000804"
     CDS             complement(33501..33695)
                     /locus_tag="IVY85_000804"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043838.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(33745..34185)
                     /locus_tag="IVY85_000805"
     CDS             complement(33745..34185)
                     /locus_tag="IVY85_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP8043839.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(34189..35034)
                     /locus_tag="IVY85_000806"
     CDS             complement(34189..35034)
                     /locus_tag="IVY85_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP8043840.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            35183..36601
                     /gene="pepV"
                     /locus_tag="IVY85_000807"
     CDS             35183..36601
                     /gene="pepV"
                     /locus_tag="IVY85_000807"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP8043841.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(36658..37605)
                     /locus_tag="IVY85_000808"
     CDS             complement(36658..37605)
                     /locus_tag="IVY85_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8043842.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(37828..38178)
                     /locus_tag="IVY85_000809"
     CDS             complement(37828..38178)
                     /locus_tag="IVY85_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8043843.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            38378..39457
                     /gene="pepA"
                     /locus_tag="IVY85_000810"
     CDS             38378..39457
                     /gene="pepA"
                     /locus_tag="IVY85_000810"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP8043844.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            39601..39921
                     /locus_tag="IVY85_000811"
     CDS             39601..39921
                     /locus_tag="IVY85_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8043845.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            39943..40407
                     /locus_tag="IVY85_000812"
     CDS             39943..40407
                     /locus_tag="IVY85_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8043846.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            40612..41217
                     /locus_tag="IVY85_000813"
     CDS             40612..41217
                     /locus_tag="IVY85_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP8043847.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(41256..42545)
                     /locus_tag="IVY85_000814"
     CDS             complement(41256..42545)
                     /locus_tag="IVY85_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8043848.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            42973..43452
                     /gene="rlmH"
                     /locus_tag="IVY85_000815"
     CDS             42973..43452
                     /gene="rlmH"
                     /locus_tag="IVY85_000815"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP8043849.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT        13750 a         7944 c         9312 g        12482 t
ORIGIN      
        1 cctgtaaata aagcaatttt atatttaaaa ataactgtga aaaaaatcac aaaaaagtgt
       61 ttacatagaa aaataaatat tgtatgatat acatgtgaat tatttaacaa ataaccatga
      121 cccattagga ggataatgat ggctaaagta atagaaaaag aaaaaacgaa gacaattgac
      181 gtacaagcaa tgattgatga tttggcagaa aaagcaaatg cagcattgaa agaaatggaa
      241 agctttgatc aagaaaaagt agaccatatc gtgcatgaaa tggcaatggc agctttggat
      301 caacatatgc cattagcgaa gatggctgta gaggaaacag gccgcggtgt ttacgaagat
      361 aaagcaatca aaaatatgta tgcgtccgaa tatatttggc ataatatcaa acatgataaa
      421 acagttggtg tgatcaatga agatgtccaa aaagggttga ttgaaattgc tgaaccagtc
      481 ggtgtggttt gcggagtaac tccaacaacg aatccgactt ccacaacgat tttcaaatca
      541 atgatcgcgt tgaaaacaag aaatccaatc gtttttgctt tccatccaag tgcccaaaaa
      601 tcttctgcag aagcagcacg agtggtacgt gatgctgcta tcgctgccgg agctcctgaa
      661 aattgtatcc aatggatcga acatccttcg atcgaagcga cttctatgct aatgaaccat
      721 ccaggtatcg cgatcgtctt agcgacaggt ggagctggaa tggtcaagtc tgcttactct
      781 acaggaaaac cagctttagg tgtaggtcca ggtaacgtac cagcttatat tgaaaaaaca
      841 gcaaaaatta aacgtgcagt gaacgatttg atcgtttcga aaacatttga taatggaatg
      901 atttgtgctt ctgaacaagc tgtgatcgtg gataaagaaa tctatgcagc agtaaaagca
      961 gaattccaag cacaccaagt ttatatcgta aaaccagatg aattgcaaaa attagaagat
     1021 gcagtgatga acgaaggaaa atatgcggtc aatccatcaa tcgttggaca ttctgcgatg
     1081 gaaatcgcaa aattggcagg aatcagtgta ccaaaaggaa cgaaaatgct gattgctgaa
     1141 ttggaaggcg taggcccaga ttatccgcta tcaagagaaa aactttctcc agttcttgcc
     1201 atgatcaaag ctaacaacac ggaccatgcg tttgatttgt gtgaaggtat gttgaatctt
     1261 ggaggattag gacatacagc agttatccat tcagaaaacg aagagttgca tgtcaaattc
     1321 ggtcttcgaa tgaaagcttg tcgtatttta gtgaatacac catctgcaga aggcggcatc
     1381 ggtgatatct ataacgaaat gattccgtca ttgactttgg gatgcggttc ttatggtaag
     1441 aactctgttt ctcgtaacgt ctctgctgta aacttaatca atgtcaaaac tgtagcgaaa
     1501 cggagaaata acatgcaatg gttcaaatta ccgccaaaaa tcttctttga aaagaactct
     1561 ctattgtact tagagaaaat ggaaaacgtt gaacgtgtca tgatcgtttg tgaccctggt
     1621 atggtccaat tcggttactg cgatacagtt cgtgaagtat tggcacgacg taaaaatgat
     1681 gtgaagatcg aagtcttctc agatgttgaa ccgaacccat cgacaaatac cgtatacgca
     1741 ggaacgaaac tgatggcaga cttcaagcca gatacagtaa tcgctcttgg aggcggttct
     1801 gcaatggacg cagctaaggg aatgtggatg ttctatgaac acccagacac atctttcttt
     1861 ggtgcaaaac aaaaattctt ggatatccgt aaacggacat ataagatcga caaaccagaa
     1921 aaaacacaat tcgtatgtat cccgacaaca tcagggactg gttctgaagt aacgccattt
     1981 gcggtcatca ctgacagtga aacacatgtg aaatatccgt tagcagatta tgcattgacg
     2041 ccagatgtcg caatcgtcga tcctcaattc gtcatgtcag ttccagcatc tgtcacagca
     2101 gataccggga tggatgtgtt aacacatgca atcgaatcct atgtttcagt catggcttca
     2161 gattatacac gtggattgag cctacaagca atcaagttag tctttgacta tttagaaaaa
     2221 tcagtaaaaa cacctgatat ggaatcacgt gaaaaaatgc acaacgcttc tacaatggct
     2281 ggtatggcgt ttgctaacgc attcctaggt atttgtcact cagtcgcaca taaaatcggc
     2341 ggggaatacg ggatccctca tggacggaca aatgcgattc ttttaccgca tattatccgc
     2401 tataatgcaa aagatccaca aaaacatgcg atgttcccta aatatgacta tttccgtgca
     2461 gatacagatt acgcagatat cgcaaaattc ttaggtctga aaggcgaaac aacagaggaa
     2521 ttagtcgaag ctttagcaac ggctgtttat gaattaggaa aatctgtcgg catcaacatg
     2581 agtctaaaag ctcaaggagt cacacaagaa acactagata caaccgtaga ccgtatggct
     2641 gaattagctt acgaagacca atgtacgaca gctaatccga aagaaccatt gatctctgaa
     2701 ttgaaacaga tcatcattga tgcttacaac ggataagcaa aagaaagcgt agaaaaaaga
     2761 taagaaaata gatgaaaaag aacttggaaa acgttgtttc tccaagttct ttttttaatt
     2821 tccaagtaac tgaaagttac ttttgttatt taacatttag gtcaatttta gacagttttt
     2881 gttcaaaaag cgatatttat agtggacggt attactgcta tattaaggat ataaaatgaa
     2941 agcgtttaaa aaggtggcgt aaagatgatt attaaagaag agatttttgt acctaaagaa
     3001 ttagtgaaag aaaaaattga cctgctcgtt gaaaacctga caacgatcaa agacgataat
     3061 ggtgaatttt tgttggactt tgatggactg aaagtagatg acaaaagctg gacagtgtgg
     3121 aattggccac aaggtgtcgg tctatatggt atttataaaa attaccagat gacaaaaaat
     3181 cctcgcgctt atcaagtggt caatgaatgg tttgaggatc gtatggaaga aggcgctccg
     3241 cccaaaaacg tcaatacaat ggcaccgcta ttgactatgg cttacttgta tgaagacacg
     3301 aaagattcac gctatttgcc ttatttagaa caatgggcag aatgggtcat gaatgacatg
     3361 cctcggacaa atgaagacgg gttacagcat gccacatatg gaccggaaaa taagaatcag
     3421 ttgtgggacg atacattgat gatgacagtg cttcctttgg caaaaatcgg gaaactgttg
     3481 aatcgcccag aatatttaga agaagctcgt taccagttta tgatccatat caaatacttg
     3541 atggataaac gaacaggatt gtggtatcac ggctggacgt ttgaaggaaa tcataactat
     3601 gccgaagcat tttgggctcg tggaaactgc tggttgacga ttgctattcc agagatcatt
     3661 gaaatcttag aattatcgaa agaagatgca ttgcgcaaat tattgatcga aacacttgaa
     3721 gcacaagtgc gtgcattaaa aacttatcag tctgaaagcg gtttatggca tacgttgttg
     3781 gatgatcctt cttcttacgt agaatcttct gcaactgcag gatttgctta cggtattttg
     3841 aaagcggtcc ataaacgtta tttaccacaa gaatataaag aagtcgctta tcgtgctatc
     3901 caaggattgc ttgaacaaat cgatgaaaaa ggagaagttc aaaacgtttc aatcgggaca
     3961 ggaatggggg acagtttaga tttttatcgg aatatcggaa tcacagcaat gccttatgga
     4021 cagtcgttaa cggtgctttg tttgacagaa ttgctcgtct cttattgtta aaagaaagga
     4081 aacccatatg aaaaaaacat cactgctgtt tgcagcaagt tcgcttagcc taggtctgct
     4141 aggtctaagc ggctgcggaa ataattctag tgtggcaact gtcgcagatg cagatgtaaa
     4201 tgagatagtt accttgcgct ttgcttatgc aagcaacagc cagccagtaa ttgatgcaat
     4261 gaatgaattt gggcgtttag taaaagaaaa aacagatggt tccgtacaga tccagtattt
     4321 cccagatgga caactgggtg gagaacgtga actgatcgaa ttgacacagt caggagcagt
     4381 agatttcaca aaagtaagtg cctcagcgtt ggaaagtttc tctaaagatt attcgatttt
     4441 ttcagttcct tatctattca atgatgaaga acatttcttt aaggtgatgg ataatgaaga
     4501 aataatggag ccaatctacc aatcgaccaa aaatttagga tttaccggat tgacctatta
     4561 tgattctggt cagcgcagct tttatatggt agacggtccg atccatacgc cggaagattt
     4621 gaagggaaag aagatccgtg tgatgcaaag cgaaaccgct atcaaaatgg tagagctttt
     4681 aggcggttcg cccgtaccta tgggaagtga tgaagtgtac acgtctcttc aatccaattt
     4741 gatcaacgga tcagaaaaca atgaatttgt cttgtataca gctggacatg gtggtgtggc
     4801 taaatactat tcctatgatg agcatacacg tgtgccagat atcatcatca tgaatgatgc
     4861 catcaaagaa cagctaactg atgagcagga aaaagccatt gaagaagcag cgaaagaatc
     4921 aacggttttt gaaattgaag cttttgcaaa agcaattgaa gaagaaaaag ctttagcaca
     4981 agaagaacat ggagtccaat tcaatgatgt agataataca ccgtttcgag aggcagttgc
     5041 cccacttcat gatatattca aaaacgatcc tacctacaaa gacctttatc aaaagatcca
     5101 agagcaagga gcgtagctgt gatgaaaatg attttaaaca aggtattaga gatcctggga
     5161 gctgttttgc ttgttgggat ggtcgctgtt gttttatggc aagtattttc cagaacggtt
     5221 ttaggaaatc caaatacggt cacggaagag cttgttcgat tcggtcttgt ctggtttgcc
     5281 atgttgtctt ccgcgtatgt tgtgggacaa aaagcacatt tagccgtaac ggttttaagt
     5341 gaaaaattaa ctggcaaaaa aagtaaaatt ttagaagttg ttgttcaaag tttatttctg
     5401 gtatttgctg cgatgatcat ggtgtatggc ggatggaatg ctgtcacatt gacaatgggg
     5461 cagatctcgc cttctttaag tattcctatg ggatatgttt atctgtcagt acctgtatcg
     5521 ggggtactca tcattatcta tagcctgatc aatctaatcg atacattaag caacaagcca
     5581 gcaattcaag aaaaaatata aaaatggagg aataaaatgg caatagaagc cggattagtg
     5641 ttattttttg tatttgttgt gttgcttgta ttagggatgc cgattgctat aagtattgca
     5701 ttctcatcga tgatgacttt attgttagtt ataccgtttg atgtttctgc ttttagttca
     5761 gctcaaaaaa tggtgggaag tatcaatagt ttttcgctga tcgctattcc gtttttcgtt
     5821 ctctcgggca ttatcatgaa taatggaggg atagctaaga aactagtgga ttttgcgatg
     5881 ttgttcgtcg ggcgcgtgcc gggggctttg gctcatacga atgtgttagg gaatgcttta
     5941 tttggttcca tctctagctc ggccattgcc gcttctaccg cgattggcgg agtgttgatc
     6001 cctcagcaag tggaagaagg atatgaccgg aagtttgcga cagcggtcaa tatcgcgtca
     6061 gctccgacag gtatggtcat tcctccaagt actgctttta tcatgtattc acttgttgct
     6121 ggtggtgctt cgatctctgc tttgtttatg ggaggttatc tcgtaggcgc tttgtgggcg
     6181 ctaggaatca tgctagtagc attcatcgtt gctaaaaaga ataaataccc tgttgtcgaa
     6241 aaaggacaga tggctcatgc tggaaaaata ataaaagagg cgattcctag ccttctattg
     6301 attgtcatca ttatcggtgg tatcttgacg gggatattta cagcagtaga agcttcagcg
     6361 atagctgtca tttactccct gttgattgct atgttttact ataaaacggt aaaactaaaa
     6421 gatataccta atatgttgtt tcaatcagta gcaatgtcag ggacgatcat gttcttattg
     6481 gctacttctt ccatgatgtc tttcgccatg gcgtttaccg gtattcctca ggcaattagt
     6541 tcgttgatta tgggcgtaac ggataataaa tttattattc tgttattagt caatcttgtg
     6601 ttattactgg tgggtatgtt tatggatgtg gcgcctgcta tcttgatttt tacaccaata
     6661 tttttaccaa tcgtcacaag tgtaggggtt gatcctgtac attacgggtt gttttcaatc
     6721 atgaatctgt gtgtaggctc catcacgccg ccagtaggta caggactata tgtcggtgca
     6781 agtgtaggag gcgtaaaagc agaggcaatg ctgaaaccgc ttcttccttt ttatggggtt
     6841 atcttagtcg tcttgttttt gattacttac ttcccgcaaa tcgtcatgtg gctacccaat
     6901 atgctgagtt aggagaatag ttatgttgaa cattacaatt agaggacatg atctttccga
     6961 agtccaaacg atagaagatc ttgcagaaaa aacaaaagaa caagggattc atacactgca
     7021 gctcgcctta ggtctttcgt ttcctgaaat accttctggc gcaaatgaga tcaattctgg
     7081 gatgggaaat tatgtcaaac gagttttaga aaagcaagaa gtgaccgtag gaattttaag
     7141 ttgctatatc aatatgattc atccagattt aacgatacga caagagttgc tgacaaagtt
     7201 cgagcagtat cttcgctatg cttcttcctt tggtgcgtcg atggttgctt cggaaactgg
     7261 atgtgttttg ccggaaatcc aatatacgga agaaaatttc acagatgaag catttgctga
     7321 agctgtgtcc gttatccgtc gcttggtaaa agctggagaa aaataccaaa tgatggtggg
     7381 gatagaacca ggtttgaatc atccggtgta ttctttagca agggtagagc agctgatcca
     7441 agcagtagat tcggactatc taggaatcat tttagatccg accaatttga tcacaagtag
     7501 tgattatcaa gaacaagtcc aattggtaga agaagctttt gaacgttttg gtgaaaagat
     7561 atgtgcggtt catttgaaag attttcgtgt cgaacaggaa aaaatcgtgc cggtaaattt
     7621 gggagacggt gtgatcgagt acacaaaaat caaagaaatc atcatgaaga atcgtccgta
     7681 cctgtatgtg gttcttgaag aaacaaaaga tgatgggatt cgttatggaa gaagtcttct
     7741 tgaataatgt aaagaagacg ttggttaact aatatttaga atggaggggg cggggctaga
     7801 agatgccacg tctcttcttt tttataagag tatataaaag cagctctttt tgattacaaa
     7861 aaacgtggta taataaaatt gtgcataagt gaacaaaagg aggtgcggca catgctccaa
     7921 aagaaaatca gcggtgaaca agtcaatacg atggtttctg atcagtatga aatctatcat
     7981 gtcaaagatg tcgtcagcca aaataaaacg atctatcatc atcatgattt ctatgagatc
     8041 catgctacat taaagggaga agcgactttt tatttgaacg gacgacaatt tacgatcaaa
     8101 gcaggaagtg tgctgctcat ccattccaat gatttgcatc gtatcgtccg ccaatcttca
     8161 gatgtattcg agcgtgtcta tatctttgtc accccttctt ttctagaaag tcgttccacg
     8221 ccatggtcta atctatcggc ttgttttcaa ccaatgggag agaagaggag tcgtgtattg
     8281 caaatagaac aggaagtatt gaagcaaaaa ttatcattta tcgaccatcc tcctgataaa
     8341 aaaatttacg gtgaagacat aaaatatgaa caagctctgg tagaatactt gatttttctc
     8401 aatcaagttg tacaaaaaga agagagtgcg caagaacaga atgctgtagt tcctaatgag
     8461 cgtgtggaac aaatgttaca atatatctca aaaaatttat ctcatccgct ttctttagag
     8521 gaaatggaaa agcagttttt tgtcagcaaa taccatatca cacgagaatt taaaaagtac
     8581 actggattta cgtttcatca gtatgtaatg aagaaaaagc tgatctatgc caagcacttg
     8641 ttgcgagaat accgcagctc tagtgctgta tatagtaaaa gtggtttttc ttcttatccg
     8701 cattttctga aagcattcaa gagagagttc ggcataacgc ctaaagaatt tttgaaacgt
     8761 gatacacaga aacagttggt tcattatgaa cattttgaag atgaacaatg agaatgatca
     8821 ctaaaaaata accgcagtag ctgctgttgt ttacatagaa gcagaaactg cggttatttt
     8881 atttttatct atatatttca aaatattttg aaaaaatgac gtacatatta tatgaatcag
     8941 ctgaatttac gaaagtctct cggcgatagt cctgtttttg ctttgaacag gcggctaaag
     9001 taagcaaaat tctcaatccc tatttcttcg gaaatcattc gaaccgtaaa ttctgtatac
     9061 cgaagaaggt ctttagcgta agaaagtttt ttgctgatca aataatcgat tggtggagaa
     9121 ccgataaatt tggaaaaatc tttgctcagc tgatatttat tcaataagaa ttgttgttct
     9181 aattgatcaa gtgtaatcgt ttctttgaaa tgttcgtcta agtattgctt cagtttgtaa
     9241 acgtaggcgg gaatatcctc ttcttcgaaa tctagctgat acttttgcaa aatcagttca
     9301 ttcagcaact cgtggatagc tacagaggac tggaaatcgg tacgggcatt cgtgccttgt
     9361 tgcatgtgga tcaattctgt gatcaaatgg tggatcttgt tttctgatgg atctcctgtt
     9421 gaatggaagg tgttatggcc gtcttttgtg aactcctgat aaaatgattg gacgttctga
     9481 ccattcaaat ggatccaatc catttcccat ggttcttcgc tgatcgtttg ataacgctga
     9541 tattcttggc agtcaataaa aaataagtct ccagctttta gctgttgctg attatcatta
     9601 tatgtaagcg aacctttgcc agacaaagta aactttatca agtaagaagg taaatgtgct
     9661 cgttcagtat agtaagggat tggtgctttg aagtaaccga tttcttggat ataaaaaaat
     9721 aaattcctgc ttctttgtgt aggcgtcaag atcactcttt ttgagtcttc cgaccatgag
     9781 tggttggctt tttcccagta atccatgtaa acactcccaa cttgtcaaga taatgcaatt
     9841 atataccaaa atagtgtgaa gcaaaatata agattctgta ttatgatagc attgtcaata
     9901 aatgataacg ctttattaat aaagtgctaa tcaacaataa agtaaaagag gtggagaaat
     9961 gaatccaaca tttgttgcca tcgatatcgg tgcatcaagc ggacgactaa tgaaaagtac
    10021 gagggatgca aaaggttctt tgatcttgga agaagtacac cggttcaaaa acggctttcg
    10081 gaacaaagac ggtcacgatc gctggaagat cgattatctt atcaaagaga ttttgattgg
    10141 atttgaaaaa ttaaagcaga cagggattga tcgccttttt gcggggatcg atacatgggc
    10201 agtcgattac tgtctgattg atgaacaagg ggatcggata aatgatccga ttgcttatcg
    10261 ggacaagcgt accaatggag cagtagaaca gttttctgaa atctattctc tagataagtt
    10321 gtatgagcag acagggattc agatccagcc tttcaatacc ttgttccaat tgtttgctga
    10381 agaaaaagaa aatttggcaa aagcacataa attgttactg atacctgatt atttagggta
    10441 tgtctttact ggtaacatgg tgacggagaa aactaatgca tctactatgc agctgctgaa
    10501 tgcagggaca aaagaatggg aaatagaact tcttgaaaaa atcggtgtct cgaaggaatt
    10561 atttccacca ttgacagagc cgggaacgat actaggagat ctgcaaaaag aacaattttc
    10621 aaaatatgat ttgccagatg tgacctttat cacagtagca agtcatgata cggcgtcagc
    10681 tgtattagga acgccaggtc aaggagaacg ttggggttat atcagcagtg ggacatggtc
    10741 gcttttaggg attgaaacaa agatcgccaa tgtttctcgc aatgcttttt ctgaaaatta
    10801 tacgaatgaa tggggtgccc ataacacggt gcgttttttg aaaaacatca tggggatgtg
    10861 gttgatccag gaagttgccc gaatgcaaga gtacgagtac agttatgcgg aattggcaga
    10921 gcttgctgca aaagaaaaac cgctgcagca agtgatggaa atcaatgacc ctcgattttt
    10981 gaatccccca aatatgatag aagagattca gcaatactgt tttgaaacag agcagccagt
    11041 ccctgtctca tcgggagcta tcgctcgttg tgtgtatgat aatcttgctc tttgttacgc
    11101 acaagagctg atcaaagtag aacacttaac tggaacgaaa ttggaaaagc tgcacatcgt
    11161 agggggcgga tcgaataatc gtctactgaa tcaattgaca gcagattatg ccggaatcat
    11221 tgtagaagca ggacctagtg aagccacagc aatcgggaat gttgccatgc aaatgctcag
    11281 tacaggtcag ttttcgaata tagaacaagc aagaaatgta atcaaacagt cgtttccatg
    11341 tgaaactttt tacccagatg aaaataagca aaacacgatg gaatggtacg tgaataaaat
    11401 caaaggagta atcgtatgaa cacgattgaa agaaattacg aacaagcaaa agaaaaatat
    11461 gcaactatcg gtgtagatac agatgctgta ctagaaaaga tacaggacat caaaatttct
    11521 atgcattgct ggcaaggaga tgatgtcaaa ggtttcttga caccggatgg cgaattgacc
    11581 ggtggaatca tggcgacagg gaattttcca ggagccgcac gaacacctga agagcttaga
    11641 caagatctgg agaaggctta ttcgttgata ccgggcaaac acaaattgaa ccttcatgct
    11701 atctatttgg atacagaaga atctgtcgat ctaaatgaaa ttgagccgaa acattttgaa
    11761 aaatgggtag agtgggcaaa aaaagaagaa atcggtcttg acttcaatcc gactttcttt
    11821 tcgcatccaa tgatgaaaga cggctttaca ttggctcatc cagataaaga agttcgtgaa
    11881 tactggatcg aacacgggaa aagaagccgc aaaatcgcgg agtactttgg gaaagaaact
    11941 gggcaaacaa gtatcaataa cttttgggta cctgatggga tgaaagataa tccgattgat
    12001 cgttttaccc cacgggaacg tttaatggaa tcactcgatg aaatttttac agaagagtta
    12061 gatgaacggt atgtacaaga tgcagtagaa agcaaactat ttggtttagg cgctgaagct
    12121 tatacagtcg gttcccatga attttatatg ggttacgggt tgactcgtga caaacttgtt
    12181 tgtctagatg ctggacattt tcatccaaca gaagtgattt ccaataaatt atcttcatta
    12241 gctttattca gcaaaggaat catgttgcat gtttcccgtc cagtgcgttg ggattcagac
    12301 catgtggttt tgatggatga tgaattgcag gaaatcgcta aagaattagt ccgtaacgaa
    12361 ttgttggaaa aaacaaatat cggtctggat ttctttgatg caacgatcaa ccgtatcgct
    12421 gcttgggtca tcggtacgag aaatacacaa aaagcactgc tgaaagctat gctggaacca
    12481 gttgagcgat tgaaagaaat ggagctggct tttgacttta cttcaagaat ggcttatacg
    12541 gaagaattga aagatttccc gtatgccgat gtatggaatt atttctgtta caaaaatcaa
    12601 gttccagttg gtttggattg gctggaagaa gtccaggaat atcaaaaaga cgtgttggaa
    12661 ttaagaaaat aggagtgtgg ctatattgaa aaaaatagat attttacaag cgccttatgt
    12721 gaaagaaatg atcgaaacga cgtcgaatct ttatcgcttg ggatgggatg aaagaaatgg
    12781 cgggaatatt tcttatttac taaaagaaga agagatcaca ccgttcttgg atcctgaaaa
    12841 agtgatccgc cgcatctcga tgatttttga tgccagccgt ttagcaggat gctattttat
    12901 tgtaactggt tcaggaagat attttaaaaa tgtagcgagc gaacctgccg agaatcttgg
    12961 aatcgttcga gttgctgaag atggcaaaac actggaactt ttgtgggggc tggaaaacga
    13021 atgccttcct acaagcgagt tgccaagtca tttcatgagt catatcgccc gtttagaagt
    13081 agacccagat aatcggatcg ttatgcattg ccatgctagt catctattag cgatgagttt
    13141 tacgcatgaa ttagacgaac gttcctttag tcgtacgcta tggcaaatgt gtacggaatg
    13201 cttagttgtt tttccagaag gaatttccat cattccgtgg atggttccgg gaacaaatga
    13261 gattggggaa gcaacagctg aaaaaatgaa agaaactcga ttggttttat ggccacaaca
    13321 tggaatctac gggtcaggaa aagacatgga tgaagtcttt ggtttgatcg agacagcaga
    13381 aaaggcagca gaagtctata cgtacgtcaa agcgcaagga ccaatcttgc aaacaatcac
    13441 ggacgaaaac ctgtggcatt tagcagatgc gtttggtgtc actccaaaag caggttattt
    13501 ggaggaagtt catacgaaag caggtgtctg acatggtgaa aaaagctttt tgcatgaaag
    13561 tttacccaga aaaacaagaa gaatacaaaa aaagacatga tgaactttgg ccagaaatgc
    13621 gtacagcact tcgtgaacac ggggtcatcg actattcgat ctatcttgat ccgagtacaa
    13681 gtttgttatt tggttactta gtgatcgaag atgaaaagtt atgggaaaaa atggcaaata
    13741 catctatcaa tcaaaaatgg tggaagtaca tggaagatat catggagaca aatccagatg
    13801 cctctccagt cagttgggaa ttgatgccag tctttgaact gtagcaacaa gcaactgggc
    13861 aaccggttgc ttggggttct ttcgttttta ttgtgaaaaa taatacaaat taggaggaat
    13921 agaaaatgag taatcgaatg attttaaatg aaacatcgta ttttggaaaa ggagcgattg
    13981 agtcgatcgt ccctgaattt cagcagcgag gattcggcaa agcagctgtc attactgaca
    14041 aaggattgat cgagcacggt atagcaacaa aagtaacaga acttctagat aaagcagcaa
    14101 tcgcttatgc tttatacgac gggatcgtac ctaatccaac gatccaaaat gtaaaagatg
    14161 gagtggcttt tgttaaagag gcagaggcag actgtttgat cgcaatcggt ggaggttcgc
    14221 caatcgatac ggcaaaagcc attgggatta ttttgacgaa tccagagttt tcagacgtca
    14281 ttagtttaga aggtgttgct gatacgaaaa atccatgttt acctatatta gctataccaa
    14341 cgacttctgg aacagcagca gaagtaacga tcaactacgt catcacagat gaagtaaatc
    14401 accgaaaatt tgtttgtgtt gatcctcatg atatcccaat cgtcgcgttc atcgacagcg
    14461 atatgatgat gggaatgccg aaaaaattgg ctgcatcaac aggtatggat gctatgacgc
    14521 atgctattga agggtttatc acaaaaggcg cttgggaaat gactgatatg ctgcatttaa
    14581 aagcaatcga aatcatcggc cattctttgg aagcttctgt agatggcgat caaaatgggc
    14641 gagaaaaaat ggcattagga cagtatattg cgggaatggg cttctcaaat gtcggtttag
    14701 gactggtaca cgggatggct cacccgttaa gtgcatggta caacattcct catggcgtag
    14761 cgtgtgcagc acttttgcca actgtcatga aatataacaa agaatatact ggagaaaagt
    14821 accgtgagat cgctcttgtt ctagggatta aaggagcagc tgaaatgtct ttagaagatg
    14881 taagagaagc ggcttgtggt gagatcgatc ggttgagcaa agcggttgga atcccagaaa
    14941 ccatttctga attaggtgta aaagaagcag atattccagc aattgcagaa gatgctttgc
    15001 gagatgtatg cacaccagga aatccaagag aaacgacagt agaagaaatc attgctttat
    15061 atcaaagttt gatgtaaaaa cgaagcggat cttcaacatt aaattgcctg taaagagaaa
    15121 tggtagttta tatctggata tctcttttaa attgatggta tttccgctat aatttttctt
    15181 ataaagaaat aggagaatat tatatggaaa ataaaaagcc ttatggaact gtattgatca
    15241 aagcctctaa aatactagac tatttagcgg aaaaccaaga tgtctctttg cagacgatcg
    15301 caaagggtgt gggaatgacc tcttctacag tcttgaaaat actagatact ttgttgatga
    15361 tcggctacgt aaataaaaat tcagagaaaa actatcgttt aggtgcaaag cttattcgct
    15421 acgcgaataa aaacattgaa caaatcgatt tggtagagtt gactttaccg tttttagaac
    15481 agctgcagca aaagatcgat gagactatcc acttaggggt attggacaac aatgagatct
    15541 tgtacgtgaa caaactagat ccaaaacatc aaacgatccg catgtcttca aaaatcggaa
    15601 tcacccgacc attatacaat tctgcgatgg gcaaagccgt attagcagaa ttcagtgaag
    15661 agcagtacac tgattatctt gaaaagcatc cgttgatccc ttacacagaa tatacgatca
    15721 ccaatgcatt acggttgaaa aaggaaatca aaacggtaaa agaaacccac atcgcatttg
    15781 acgatgaaga aatcgaacgt gatatttttt gtataggagc ctcaatcgtg aaaaacgaac
    15841 aaatcattgg tgcatttagt gtaagtatgc cgaaatatcg tttaacagat gagacaaaag
    15901 aacaaataat cagtgccctc aagcaaacaa aaagcgaaat cgagcaaaga attcaaaaat
    15961 aatgaaaaaa gcgtttgcaa tttttctttt ctgccgtata atatgggtat agatttccaa
    16021 gttataaaat aaaatttcta aataggaaat aggtggagaa gatgaaaaaa attgatattt
    16081 tatctcgatt gaaaaacgcc ggagtgattg cggtggtacg cggaaaaagc aaagaagaag
    16141 ctttgaatgc ttgccacgca atcatcaaag gtggtctaac tggtattgag ttgactttta
    16201 cagtccctca agcagatcaa gtaatcaaag aacttttgtc attctacaaa gaccaaccag
    16261 aaatagtgat tggcgcaggc acagtattag acgctgtcac agctcgcttg gctatcttag
    16321 ctggcgcaga gtatattgtt agcccttcct ttgatcaaga aacggcagag atgtgcaatc
    16381 tgtatcaaat tccttactta ccaggatgta tgactattac cgagatcaaa acagcgctga
    16441 aaagcggggt agatatcgtc aaactatttc ctggaagcgc ttatggacca agtatcattt
    16501 ctgccttcaa ggcgccgatg cctcaagtca atatcatgcc aactggcggt gtgagtctgg
    16561 acaatatgaa agagtggttc gatgctggtg tcgtaacagt cggagtaggt gggaaccttt
    16621 tagcacccgc agctacagga gattttgaca aagtaacgga agtcgcgcaa cagtatgcag
    16681 caaaaatgaa agaaatcaag aggtagctgc ttatgggaaa agtagtgaca ctcggagaaa
    16741 tcatgttgcg attgtccaca gagcagggaa cccgtatcga ttcaagtgaa cgattcgctg
    16801 cccattatgg cggaggggaa gcaaacgtag caatttcttt agctaattac ggacatgaag
    16861 cgatttttgc tacaaaagta cctgataacg cattgggaaa agcagtcaaa aagcacttgc
    16921 aatgttatgg agtagatacg aaacaggtat tatctggtgg atcaagacta ggtacttatt
    16981 atatggaaac tggtagtggc gaacgcgccg cctcagtgat ctatgatcgt gccggatcaa
    17041 gttttgctga aatggatcat ttggaatggt cgctagagga gttattccaa ggaatagata
    17101 tatttcatgt ttcaggaatc accccagctt tgtctaaaaa atggcaaaaa atgacgaaac
    17161 aggtgataga agctgcaaaa aaagctggct gcctgatcag cttcgatatc aactatcgtg
    17221 gaaaattatg gacacagcaa gaagccagcg aagtaatcca tcagctttta cctttagtag
    17281 atatctgctc agctggaaaa atggatgctc tttatttgtt aggtatcgag aaagcgccag
    17341 aagcagaaga acaacctcta atttattatt atcaaaaaat gcaggaaaag ttcccgaata
    17401 ttcaggtgtt ttattcgacc aagcgtaccg ttcattcagc aagtgaaaat gaattgaagg
    17461 ggacattgtg gatgaaccat gaatatgtgg aatcccaagt ccatagaatc accccaatcg
    17521 tggatcgagt aggtggcgga gatgcttttg caggtggcgt gctccatggg attttatcca
    17581 atatgtctcc gcaagaaatc attgattttg ctacggcagc ttctgccttg aagcatacga
    17641 tccatgggga ctgcaaccaa tttagtcaag aagaagtcgc acaattttta gcatgcggct
    17701 ctggaaaaat cattcgatag aaggagtaga agaaaatgca agaaatggaa acacgttata
    17761 cacacagtcc agaagatatc cgtcattatt caacggaaca attgagaaaa gaatttttag
    17821 tagaaaaggt atttgaacca gggaaaatca gtttgactta tacgcacaac gatcgaatga
    17881 tctttggtgg agtgacacct actgaaaaag aattagaaat cacattagac aaagaactag
    17941 gtgttgatta cttcttagag cgtcgtgaac taggtgtgat caatatcggt ggaccaggat
    18001 tcatcgagat cgatgggcaa aaagaagaaa tgaaaaaaca agacggatac tatatcggaa
    18061 aagaaacacg acatgttgta ttttcttctc aagatgctga aaacccagct aaattctata
    18121 ttagttctgt tccagctcat cataaatatc caaatgtaaa aatcagtatc gaccaagtaa
    18181 aaccaatgga gactggtgaa ggcctgacac tgaatgaacg taaaatctac caatacatcc
    18241 atccaaatgt atgcgaaagc tgtcaattac aaatgggtta tacgatttta gaaccaggaa
    18301 gcgcttggaa tacgatgcct tgtcatacac atgaacgtcg tatggaagct tatgtctact
    18361 ttgattatgc caatgaagat acacgtgtgt tccacatgat gggcaaacca gatgaaacaa
    18421 aacatttagt tgtagacaat gaacaagcca ttatttcgcc aagctggtcg atccattcag
    18481 gtgtaggtac aagcaattac tcattcatct gggcaatgtg cggagaaaat atcacctata
    18541 cagacatgga tatggtgcaa atggatcaat taaaataagc ataagggaga gaaaatgatg
    18601 gaatttagta tggatatgtt tcgtttagat ggaaaagtag cattagtcac cggtgctgtc
    18661 tatggtattg gatttgagat tgcacgctct ttagctacag caggagcaaa aatcgtattt
    18721 aataacttga atcaagaatc agttgatgaa ggtatagcgc attataaaga agcaggcatc
    18781 gatgcaaaag gttatgtctg cgatgtgacc gatgaagaag cggttcaagc gatggttcaa
    18841 cagatcaaag aagatgtagg ttcagttgat attctagtca acaatgcagg gatcatcaaa
    18901 cggacaccga tgatcgaaat ggatgcggca gacttccgcc aagtaatcga cgtggattta
    18961 aatgccccat tcatcgtttc aaaagcagtt attccggata tgatcgaaaa aggcggcggt
    19021 aaaatcatca atatctgctc catgatgagt gaattaggac gcgaaacggt cagtgcttat
    19081 gctgcggcaa aaggcggatt aaaaatgctg acgaaaaaca tcgcttctga atacggtcaa
    19141 tacaatatcc aatgtaatgg aattggacca ggttatattg ctacaccgca aactgctcca
    19201 ttacgcgaaa cacaagaaaa cggtgaacgt catccattcg atcaatttat cgttggacgt
    19261 acgccagcag cacgctgggg cgagccaatc gatttggctg gaccatctgt cttcttggct
    19321 tctagcgcat ctgatttcgt taacggccat gtcttgtatg tggacggagg catacttgcc
    19381 tatatcggaa aacaacctta aaagagcttt agtacactgt ttagtggaga ggttgtgaaa
    19441 tcagcggatt gaacgaatac cgaaggacag ctgattaaac atcatttatt cgaaaaaaga
    19501 ggctgggaca gaagcattta actccaagaa ataagaagga attcacgaaa attgcttttc
    19561 aaatttttgt gaatttcagc ttatttccga aggagttgct tttacttccg ccgtttgtac
    19621 gtgtttagag cgtgagacaa aagtgttctt tacttttgtc ccacgctctt ttctgtgcgt
    19681 cttccatact tcactaagtc gatccctctt gcttcgcacc aatccttcac tggtccggaa
    19741 aagatcagag attgttggtg tagggccgcg gtattcgttg cgccgaccat agtataaagc
    19801 agacgaagct cttcttgcca ttgcttcatc agtataatag tttcttctac gccgtgattc
    19861 atcagatgcg tcagtacggt tcctgaagta cccacggcat ttgcgccgag acaaagggct
    19921 ttaaatatgt cataggcatt gcggatacct ccggaggcta ggatttccat agaagtgtct
    19981 gcttcatttg cttcaagcaa tgaagagact gtcgattgcc cccagtcagc taaataactc
    20041 agttcccgct tggagcgacg ggcgttttcg atctgggtga agcttgttcc gcttcgaccg
    20101 ctgatatcaa ctgtatgaac tcctaaagca gctagatcat tgagcgtttc tcttgtcatg
    20161 ccaaaaccga cttcttttac gatgaggggg acgtcaatgg ctgtttgagt ttcttgaata
    20221 agtactttcc aattggtaaa atcacggtct ccctcaggca taaccaactc ttgaggtgca
    20281 ttcaaatgaa tctgtaaggc atctgcgtga aacaatcgga tagcttcttt ggctctttcg
    20341 acagaagtcc ctgcaccgat gttggcaatg attttcccgt gaggatattc ttgtcgcata
    20401 atcgtataag tatcggctaa agaagggact ttcaaggcag cgctgactga accagtggca
    20461 atcatcaaat ctgcttctct tgcgacgatt gcaagatctt ggttgatttt ttttgttttt
    20521 tcgcttccgc cagtcatcgc attgatataa aatgggctgc ttagtgtaaa cccaactgcc
    20581 tgtgtactta gatcgacatc agaaactgct atttgcggca aagggttatg gatgatccgt
    20641 acgaagtcaa attcgttttt ttgtttatca tgaaaggctt tagctagtga aacatgttcg
    20701 tcttttcgat tcatcggttt tcctttcttt gatcgctata gacatgtaac ggcagtggag
    20761 tgatttctgc tttttcccat gcactcatta aaggaagaat gccagatttc tggtcaacaa
    20821 ttacgattcc gcaatcgccc ccacctgcac cagaagattt tgcggctcct tcatactgtt
    20881 cagctaaatt acacaatttg ttcaaagcag gcgtttcgat gacgacccca gtgattgcag
    20941 ataaatcatg cagtaattgt cggttttttc gaatcatctg ttggatcaac gttacattat
    21001 tttctttaaa ccctttgatc atttcgttga cacattctgt actatttttt aagaaaagct
    21061 gataagccac cattttatct tctctcgaac ggtgaacttg atcgaccaaa tcagaagtag
    21121 aggcagggct acccgtccaa ccaatcaata aacgtaaatc ttcaggagca atcaatggct
    21181 caatggatag acctggccaa tccagggcaa gtaactcact gatagaatgc tgagtttctt
    21241 gttcttggag ccaaggatgg tcgaaggttg agaaagcgat ccagccacca taacagctag
    21301 cagcgatgtc gccgcaggaa ccattatctt gaactgctaa attggctagt gctgcaatct
    21361 tgaaaatctc caactgagat aaattcaacg cgtaaaaaac attcaaggct ttgaccgttg
    21421 caacggtcac ggcaccgcta gaacccaatc catatttccg gccatttgaa ctgtctaact
    21481 cacttgttac tttcagatca taaaaggata aaagaatgtt tttttcttgt gcatactttt
    21541 cagtcaagcg aatagcagca aggatataat gaaaaggatt ttcccgaata tctaaaacta
    21601 attctccgtt gcgtcttgtc caacgtacag gcatcccact atattgtgca gattggatac
    21661 ttccgacttt tcgtgcagat tctacagtga ctgtcacgaa ttgatcgact gcagcgataa
    21721 ctgctggatg gcctgtttca acaactgcat attctccggc aatatagagt ttgcctggtg
    21781 cagatacttc aatcataaaa atctcccttt attcaataat cgcaattcct ggaccagcat
    21841 gtgcagtaat tacttgttct ttgctaaata atttggtaaa tgtttcttgt acctcagata
    21901 aatggctgtt ttccactaaa actttgacat tagggccagc atccatcgta aagtagcatg
    21961 gaatgccttg ctttctcaat tgtcgcaccg catccatcgc ttttaagctg tcaggagacc
    22021 agtaagtgaa tggaggctgg gcagctaaag tcgttccatg catttttagt ccgtttcttt
    22081 ccatcgtttc tccgagaagt tggaaatctt ttgttttgat tgcttgtttc aattgatata
    22141 gatccccttc aacggaatct aaccagcctt gataaaaact ggatgtttcg actgtccgac
    22201 gcatcccatt tctgctggac acttcttttt tctggtcgtt gatcaaaacg aaaaccattg
    22261 ctaaatcgtc ttcgaaagaa tcggaaggga ctggcttagc gtaagaactt aagtcgtcat
    22321 ggcctttttc ccattcgacg aaaccaccga aaatacttcg gcaagctgaa ccagacccgc
    22381 gtcgagcaaa tcttgaaaga gagagatcat ctaatccaag cttaagagca gtattgcaag
    22441 cgcctgctaa agcagctagc ccgctggcag aggaggctaa tccagctgcg gtaggaacga
    22501 aattctgact gattactgaa gcttttagtg agaggccggc ttctttacga aaaaggtcaa
    22561 gaaactgact gacttttttt gttgcttttt cgtcttgcaa ggtgccatct agataaaatt
    22621 catctaccat atagctatca gaaaagatga cttctgtctc tgtataaaat gcatccaatg
    22681 ttaacgaaag gctgttgttc attggaagga tgagttcttc atttttctta ccccaatatt
    22741 tgattagagc aatgttggta tatgcgcgtg ctttgccttt aaacataggt atgtactcct
    22801 aatccttgta tccaagtttc agcagcaccc gcatcttcaa gtgcttgact gatttcttgg
    22861 gcttttgttt ttgtttgtgc taatgcaatc atacagccac cgcgtccacc gccagttaat
    22921 ttggcaccca atgccccggt atcttttgct gtttggatca atagattaag aaaatcattg
    22981 ctgattgtca gcttttccag aagtgattgt gattcatcca ttgtctgtgc caacgtttct
    23041 ggtgaatttt caatgattgc ttgttttgct tgcttcgtca agtatcctaa tttttggatc
    23101 atttgtccag tttcatgagg ctgggtttca aagaggtgag cgacatcttt gacggcttct
    23161 cttgtttgtc ctttgattcc tgtatcagcg acaatcaaaa aagcatcgat gttcaaagaa
    23221 aagtagtcga aaggatgccc ttttgtaaaa taaatcggct gcaagctgct agtagcggct
    23281 gcatcgattc cactaggatt accatgggcg attttttccg aaagctggac attttctaat
    23341 agtatttcac gagacaaagg aaatgctaag taatcataaa aagcacgagt gactgctgtg
    23401 gcgactgcag cgcttgatcc cattccacgt tcagcaggaa tcgtgctttc aatggtcagt
    23461 tgcaagtttt tttggatgtc atgctgtttt tttaagtgaa tgaataattt tttgatattt
    23521 tttaatgcat gaggagcttc ggctaaattt cctgaatagt aggaagaaac cagttgatcg
    23581 tccattgcat ccagctcttc aaggaatgcg gtgacttttg ttgcatagaa aggaaaggca
    23641 atcgctggtt ctccataaac aaccgcgtgc tcgcccatca atatgatctt tccgcttgat
    23701 tctccttggc catagtttgc cataatctaa ctccttctca cgtaaaagtg atttttaagc
    23761 aaaaaataaa gcgcccaaaa cgagttgcgc tgcttcacga atttttttgt ccactaaggg
    23821 tctcaaaaaa cattcctcct ttattttact aaaaccatct cagaagtaaa actaagaata
    23881 cgttttctaa agaaaaaaca cttttttctt aagaattttt tgactttttt tcttctttga
    23941 ctagaaaaaa atacaattga cactactaga tattgggggt atatttaaaa aggttggagt
    24001 taaacacaat atttagtgat gaagtgagct ggaggtaaat gagcgtgatc gttttagcag
    24061 gtacaattgg tgccggaaaa tcaagtttaa cagaaatgat ggctgaacac tttgatagtc
    24121 aggcatttta tgaatcgatt gatgataatg aagtcttgcc cttattttat gcaaaccctg
    24181 aacagtatgc ttttttgctg caaatttatt ttttgaataa acgttttgca agcatcaaac
    24241 aagcaatgaa agatgacaat aatgtattgg accgttcgat ttatgaagat tcactgcttt
    24301 tccatctaaa tgcagatcta ggaagagcaa cagaaacaga agttcgtgtg tatgatgaac
    24361 tgctggaaaa catgatggaa gaacttccct atgctgccca taaaaaacat ccggatttat
    24421 tagttcatat tcgtgtatct tttgatacga tgttggagcg tatcgaaaaa cgtggtcgtt
    24481 cgtatgaaca attgtccttt gatccaagtc tatacgatta ttacaaagaa ttgaacagac
    24541 gttacgatca atggtatgaa gaatacaaag aaagtccaaa aatccaaatc gatggagacc
    24601 gctataattt tgtggaagat cctcaagcaa aagaagaagt cttgaaaatg atcgaagaaa
    24661 aattagcaga gattcgccaa accaaagtag ccatttgatt tacagttgct caaataaagg
    24721 gtaaaccttt atttgagcga cttttttctt tatactggaa acaaatgtca ggaggataga
    24781 gtatgcggga aaaacactat tttaatcaag accgtaattt ttggatcaat gtacagcaaa
    24841 acgatgtgct agctgttaaa aacttaaaag aagaatatgg cattagtgac gagatgctga
    24901 cttattctct agataaaaat gaacgggcgc gcgtagagta cgattctttt gatgaagcgt
    24961 tactgcttgt atcaaatgtt ccgcaccagc aaaaagtcga taatcattat gaaactagtc
    25021 ctatcgcttt tattttaaaa gaagacggtt tgtttacatt taccacgccg aataccgagt
    25081 acgtgattcg actaattcga tcgcttttgg aacgaatgcc agacatgagt gtatacagtt
    25141 tattgtttcg tacattgttt ttgatttctg attccttttt tcctttaatc gaggaagtga
    25201 atagtgaacg tcagcggctt aatctgaaat taagagaaaa aacaacgaac aagaatctgc
    25261 tgcaactttc agatttagag atcggtttgg tttacttagt tacaggaaca aaacaaaatg
    25321 tagtactttt agaacaaatc aaggcattgg ctatttatcg caagttgtca gaaaaggaaa
    25381 aagaagagtt ggacgatgcg ctgatcgaag cacgacaagc agttgaaatg acgaacttag
    25441 cttcgcagat tttagaccag ctctctggca cttataataa tttgctgaat aacaacttga
    25501 atgacacgat gaaatttttg acggtctggt cgctgatctt gaccgttcca actattgtga
    25561 caggattctt cggaatgaat ctccagttac cctttaccca ttccgttttc ggatggggaa
    25621 tcgccttgat catcagtttg gtcttatcta tttggatgct tattgcactg tggcggcgga
    25681 ttcgttgatc tagtctttga tctgcaaacc aagggcttgg gcgatcacaa gtgcttgctc
    25741 ttggttgacg attagtcccg ccaaagaatc ttgagataaa gcgattgctg tgaagaaatt
    25801 agtcgtaaag tctaatccct tcagagaagt tcgaaaccag ttcgaaccgg tcaactgatt
    25861 gtcagacaaa aaaaggtttt tccaagtgac ctcataaaat tcgctgtctt ctaattgaca
    25921 atgatcgaag gagacagctt tcaagtttgt actactaaag gaagccatcg aagcaataca
    25981 atcagtgaaa gtacagtctc gaagataact ttcggcaaaa ttcgtacctg ttaatttaca
    26041 ttgatgaaac acgacttggt ggaaactgcc accaatccac tccagattgg aaaaatcaca
    26101 tttttcaaaa atgacattac tgcattcaaa atgacgtaaa actgtttttt gcatcgtaag
    26161 tttttggata tgactctctc ttaataccat atttttggca tcgagatagc tttggtcacc
    26221 cccgttgatc agaagaccag agaagatctc ctcatcttct aaataaaact ctccctttgt
    26281 aagaagagga agagaaggtg gaataggttc gatttttttc atgtgggttc acctcgtttt
    26341 tctttagtat agcaaagaag cgggaatcac tgctagaaag tctgctaatc taatcatttt
    26401 cagaaaagga agacaaactg tatgaaaaac attgacaaaa agattagttt ttgctatact
    26461 atgatggttg agaaataaaa gcagaagcac ccgcttctcg ccttagttga taatgtcact
    26521 gggctagata gttttcactg gttctattag gtgaagtacg cgcgggttct gtaagagaac
    26581 tcgttttttt attgccgttt attcggtaag cttttctttc tctcataata tttggaggtg
    26641 aatgaccata gcaaaggata tgatggttaa cgacggcatt cgtgcacgag agttacgatt
    26701 gatcggttca gatggtgagc aattaggagt taagacaaaa gcagaagcat tgaatattgc
    26761 agaacaagca aacttggatc ttgtgttagt tgcccctggt gcgaaaccac cagttgcgcg
    26821 aatcatggac tacggtaaat accgtttcga acaacaaaag aaagaccgcg aagcgcgtaa
    26881 aaaacaaaaa gtgatcaatg taaaagaagt tcgcttaagt cctacgatcg acttgaatga
    26941 cttcaataca aaacttcgta atgcacgtaa gttcttggaa aagggcgata aagtgaaagc
    27001 ttctatccgt ttcaaaggcc gtgccattac ccataaagaa attggtcaaa aagtcttgaa
    27061 tcgcttagct gaagaaactg cggatattgc aacagtggaa caaaaagcga aaatggacgg
    27121 acgcagcatg ttcttgacgc tggcaccgaa aaacgacagt aagtaatttg aatgactaac
    27181 tggctgttgg acagccgata gtcggataac gaaagatttt gaggaggaaa tatagtcatg
    27241 ccaaaacaaa aaacacaccg cggatcagca aaacgtttca aacgtactgg taaaggcgga
    27301 ttgaaacgct tccgcgcgtt tacaagtcac cgtttccacg gcaaaacaaa aaaacaacgc
    27361 cgtcaattgc gtaaagcaag catggtttct agtggcgact tcaaacgtat tcgccaacaa
    27421 ctagcaaaaa tgaaataaga agtacaaata acagaatctg aagatttatt tttattagga
    27481 ggaattatac atggcacgtg ttaaaggtgg aacagtaact cgtaaacgtc gtaaaagagt
    27541 gctaaaatta gcaaaaggtt actacggttc aaaacacact ttatttaaaa cagcaaaaga
    27601 acaagtaatg aattcataca actacgcata tcgcgatcgt cgtcaaaaga aacgcgactt
    27661 ccgtaaattg tggattgctc gtatcaacgc agcagctcgt atgaatggct tgagctactc
    27721 aaaattaatg cacggtttga aagttgctga aatcgacatc aaccgtaaaa tgttagctga
    27781 cttggctgtt aacgatgcag cagcatttac tgcattggct gaccaagcaa aagaagcttt
    27841 ggctaaataa gctaaacaat tataaagagg aaagaagata acgaaaagtt atgttctttc
    27901 ctcttttttt atttacaatc ctccatcaaa tgaaacagga caataacgta taagtctaaa
    27961 atagcataat taaggttttt taattgaaaa ctgaacattt atggtcaccc tttaatgaaa
    28021 gcgctaacta cataagttag aatgagtttg actgatatat tagtttgctt atcagaaaaa
    28081 caaaatggac ggaggaataa catgagtgat attattattg gtattttgtt agttatctca
    28141 ttctttttca tggtctggta ctgtgtaaaa ggctacaact tgatggtcgg ctttgccatc
    28201 atggctactg tatggatggg attagcgcta gtcggaaaca cgttttcacc aaacccggcg
    28261 atggaagggc aaggagtaat tgatgtgttg acgcatatct atacgacggg gccagctgag
    28321 tacgccaaat cgatcttggt aaatgtcttc tttggtgcct ttttcgggcg tgttctagta
    28381 gatagcggaa tcgcagctac cctgattcga aaggtagttg aacttggagg ggacaagcca
    28441 agaatcacga tgagcttact atgtattgta accgctgtta tattcatgtc gatgacagga
    28501 atcggaccag tgatttctat tgcagttatt gtgttgccaa tcttgatgtc attaggtatt
    28561 tcagtaccgg ttgcactgtt ctcatttatg ggctcgatta tggctggaat ctttgctaat
    28621 atcgtcaatt tcaaacaata tcaaacgatt tatgctggtt tcaatccagc ggctgaaagc
    28681 tatacttata atgactattt ccaaattggt atgattggga tgattgtaag cttagttgtg
    28741 gtactaacgg tcgctaatat atcaatgaac aaaaagaaac gctatgcaat ggcagcaaac
    28801 gtaccagctg aaggtggcga cgcaccaatg atctcatggt tagccgtatt attaccagtt
    28861 ttaggggtcg ttcttttaga tcttccaatc atcttaggtt ttattctagc aggtatctgg
    28921 gcattgctgt ttactggaaa attgcgcgga ggttataaag aaatctgtcg tcagtttgcg
    28981 aaattattta cagatggcgc agtagatgtt gcgccaatgg ttggtttctt gatgacctta
    29041 gcgatgttca ataattcagc tgcgtatgct tctccttatt tttcagctat ttttggagat
    29101 tggataccgc aatcaccatt agtcttggca atcgtatttg ctatattgac accactagga
    29161 tttttccgcg gtccaatgaa tctagtcggt tcaggctcag ctattttagc tgtcgtatta
    29221 gctgttaatc caacgatgtc gccagccttt ttgttcccgt tatttgcaat cacaacgatt
    29281 gctcctcagc atttagatat tacacaatct tgggttgctt ggggattagg ttatacaaaa
    29341 gtaacttctc gagaatatat gaagaaatcc atcccaactg gttggatcat cggagcgatc
    29401 ttatgcctga tcacgttctt attgtacggg aacgcctagt aaacaaaacg tcgataaaaa
    29461 cgattagatg aatggaggaa aagaaaatga aacgttttgt gcgaatggga attgatgttg
    29521 gcggtacaca tacgaaagca gtcgcaatcg ataatgcgac gcacgaaatc atcggaaaat
    29581 cttcagtgaa aacaacccac gatgatgtcc gtggagtagc cgcaggtgtc gttcagtctt
    29641 ttcaaaattg tttgctggaa aataatatca gtccagaaga tgttgtgttt gttgcacatt
    29701 ctaccacaca agcgactaat gcactgattg aaggagacgt agcaaaagtc ggcgtaatcg
    29761 ggatggcaaa aggtggatta gaaggttttt tagcaaaacg acaaactaga ttgaacgata
    29821 tcgatttagg gaataagaaa aaaatagaga ttgtcaatgc attcttacct gtcaaacact
    29881 taaatgtaga ccgtgtttca gaaacgattt cttctttaga aagagaaaga gcggaggtct
    29941 tggtttcttc tatggcattt ggagtagata atggggaacc agaacgagtt gtttatgaag
    30001 cagcatcggt aaaatcgatc cctacaacga tggcttctga catcacaaaa ttatatggat
    30061 tgacaagaag aactcggaca gcagcaatca atgcatctat tttaccaaaa atgctagata
    30121 cggcaacctc tacagaagat tccgtacgag aagcaggggt caatgtttct ttgatgatta
    30181 tgcgcggaga cggcggagta atggaaatca atgaaatgaa aaaacgacct gttttaacca
    30241 tgctttccgg tccagcagca tcagtcatgg gctctttgat gtacttgaga gcgtctaatg
    30301 gtgtttattt tgaagtaggc ggaacaacaa ccaatatcgg ggtcattaaa aatggacgtc
    30361 cagcaatcga ttattcaatt gtaggtggcc acccaacata tatctcttca ttagatgttc
    30421 gggttctagg tgtggcaggc ggttcgatgg tacgggctaa tcagtcagga atcatcgatg
    30481 tcggtccacg ttctgcacat attgccggtc tagattatgc agtatttacg gagacagaaa
    30541 aaatcaaagg tcctaaggtt gaatttttct ctcctaaaga aggagaccct gctgattacg
    30601 tcaaggtagt gatggaagac ggagaagaag tgactattac aaatacatgt gcagccaatg
    30661 tccttggatt agtgcaagaa gaacattttt cttatggaaa tgtcccatct gcacgaaaag
    30721 ccatccaagc attagctgat tattgtcata cgacggttga agacatcgca gaacagatca
    30781 tggaaaaatc ctatgctaag attgaaccgg tgattttaga attggcagac aagtatcact
    30841 tggagaaaga tcagatttct cttgtaggtg tagggggcgg cgctgcctca ttgattactt
    30901 actttagtaa taaaatgggt gtgaaatatt cgattcctga aaacgccgaa gtgatttctt
    30961 ctattggtgt agcacttgca atggtccgag atgttgtaga acgtatcatc ccttcgccaa
    31021 gtaaagaaga tatacgatcg ttgaaaaatg aagcgatgaa taaagcgatt gaatcaggcg
    31081 ctacaccaga atcgatcgaa gtccacgtag aaatcgatcc acagacttca aaagtaacgg
    31141 ctatcgctac tggttctacg gaagtgaaag caaccgatct aaccaaggag atcacaacag
    31201 aagaagcact agagcttgct gcagaggata tgcgattaaa taaaaatgaa gtttgcttat
    31261 tggaaaatac acctttcttt tatgtatgcg gcgaacagaa tcgttcaaaa aatgctggaa
    31321 gtcttcggat catcgatcaa aaaggattta tcaaggtcca acgtggacat gcttcatgta
    31381 tgaaaacaac tgcagccaat tatatgacag ctgtcgaaca actttgggaa gatatggcag
    31441 tttatcaaac tgaattgatt gcccgaccag aattttatct ctgcttagga gcaagagtct
    31501 ctgattttac agcgacggat ctggaacaac tacagctttt gatggatctg gaagtatcta
    31561 ctatggaacc ggaggaagaa gtgatcgtcg ttgccggaaa tatcaaacaa acataagtta
    31621 gaagagttac taaacgaatt acaacagatc cccgaagaaa tttggggatt ctaccagttt
    31681 cggcgagatt tattttggaa aaagattcct ttgtccaagc agaaaattct tatacaacaa
    31741 tcaatcgatt gtggtatcga aacggcatgt agcatcaaaa aaaagtaccc ttttgccgat
    31801 gtaggagaaa tttgtgaaca aatggctgtt ccaatcgttt cctgtgaatc agagcaaata
    31861 aacgaacgaa tcacttttgc aacttatgca gaagatgaag ggattcgatt gatgacggag
    31921 ccgttagaaa aattaaaatg ttcaggacta gcttcgatct ctaaagaaac agcgcaagct
    31981 ttgattattg gacatgaact atttcaccac atcgaagctt ctgttaaggg aatttatacg
    32041 caaagcgaaa aaattgtgct gtggcggttg cctttttata cacatcaatc aaacattcgt
    32101 gcattgagcg agatagcagc gatgagtttt tcaaaagaga tgaatcaaag cgatttttcg
    32161 ccttatgtat tagaggcagt cttactttgg ccatataatg aaattcgtag ccaagggatc
    32221 ttagaagaaa taaaaaaaat agaaaaaaga tgtacggagt atgatttaga ttacaaataa
    32281 agagactgtg acaaaagtcg tgtcacagtc ctttttttcc gaataaacga tgttctcctg
    32341 tcaactcctc ggtcacaagt caaaatattc aacaaacatg agaagctgtt ttttgaaatt
    32401 gttccttgtg actcggagat gaacggcagg ctcacgacct cttggaaaac ggtgttcaaa
    32461 agcttgcatc tgcggtaata agtccagcca agttaaaaac ataagaagct atttttactt
    32521 ggctgttctt attacttgat gcagggcagc tttttcacaa cctctcaata aacggtgtgc
    32581 aatcagctga ccctcggtat tagctcaaaa aaaacggcaa aatatgagga actattttgc
    32641 cgtttctttc tctaatactc aggtcaaaac gctgattcca aaacctctta tttgatgggt
    32701 ctgtcctgaa tatcttcaaa ataatcgggg taattatgaa tcacgtattc ttgctcctcg
    32761 ataatcatat gtaaatgcat cgtgcaaaga aatccgactt catcaaagtt atgatcaatc
    32821 attgattgaa gcattgtctg atgttcatct aatactcgac cccagtctaa ttctgaaatc
    32881 gtcaaacgta accatctgaa acgcgtacta taactttcta gccaatccca aatttctcgt
    32941 tttccagcaa tttcataaca agttcggtga aaatcagtat ccaaatcaaa aaaagctttt
    33001 ttattttgag cgattactgc ctgatcggtt tgttctagta ttttttctag tttagcttga
    33061 tcttgcttgg tcattttcgc actgcattct tgcaggatcg gtttttccaa cttttcccgc
    33121 gtataacggg ctaatgaagc agatcggata tcgatttttg aaacataagt gccggactgt
    33181 ggaatggttt ttacaagctc ttgattgcgc agttggatca gtgcttctcg aatcggtgta
    33241 cgcccgatag ctagttgttc ttctaacact ttatcggaga ttttacttcc aggtgctaac
    33301 tcggaataaa tgatttgttt acgtatagat tcataagctt gctgttgcaa attattgata
    33361 accatttaac cttcctttct atgcagatat aatctattat aacataaatc gcttacaaat
    33421 cgtgtgggca taaaaacaca gtctgcaaaa aaagccctga taggcatgcc tattagagcc
    33481 tcgaaaagaa aaatattgaa tcaatctggt cgtacctctt ggatcggttt gacacgaccg
    33541 ccgattcttt tagcaaaagc ttcggattct tttttgtctt tgaaaatgag cagattgtct
    33601 aagttttttt cccgagtgcg acagccagtt ttatcgatgt aaccgtgcat tactttaacg
    33661 acatacatgg ggatcacctg cttttttctt ttcattatac gaactactcg acacaataca
    33721 agaaaaatgc ttagacggtc tttattaaga atttacatca tccaaagaag cgtacgcttg
    33781 gaataactga tctaactccg gattgacttt gctcagcttc actaatcgtt gcgaatcaga
    33841 aagtaaaaaa cagtgtttac tactgccagt agtttttaat tttttgtctt cagttcccac
    33901 aatttcgtag ctaaaatcca accgtgtccc cgtatacttt tggatagttg gatggatgct
    33961 cactttttct ccgtaatgga tcatttcttt atagctgcaa tttacttcca gaacggggat
    34021 gatgatccca gattcttcga tttttttata gggaaaccct agatgtacca ataaggctac
    34081 ccgagcctct tcaaaccagc ggatataatt gctgtgatga ataatcccca tttgatctgt
    34141 ttcataataa aaaggttctc gcacataacc tgtaaatttc tccatgactc atgcctcgtt
    34201 ttccattgat tttttacaaa aataattcat ccatttcgga agtgcttcgc tgatttgtgt
    34261 cggcaaaaca atatattgtt cttttgccag ctggtctcca atgaaactat gaagataaat
    34321 agctgctgca atcgtttggt cattttgagg aaactgggct aaaaatcctg tgatcatccc
    34381 agctaaagtg tcacccatgc cgcccgttgc catagcagca gaacctaaag gattggtgta
    34441 gaagtgctgg tgaccataaa tttttgtctc atgacttttc aaaacgagca cagcaccgat
    34501 ttcctgttgc ttctttttgt tttctttatc ccgttgttct tgtatcggta gagaactcaa
    34561 ccgttgccac tccatttgat gaggtgtaag tacagtttgt tcgggatagg gtagcaaaag
    34621 atgattagca gcaagtaaac ttaaagcaga gccatcaatc accagccatt gttcttttga
    34681 ttggttttct agtacttgtt tgaaacgttc caaactctta gaagaagtac ccaatccggg
    34741 accaatcaag agtacatctg cttgctttat tacggaaaga aactggtcag tatccgtcca
    34801 aactacaatc attgcttctg gcagatgact gtgaagagca ctccgatttt tttcatcagt
    34861 tactacagtc gtgagacccg ctccgctgtg tacggcagct aaagcactca taatgattgc
    34921 tccgccatat tgttcattgc cgccaatcaa aacgatacgt ccaaatgttc ctttatgtga
    34981 aatttttgga cgaggtgtga tgactctttt cactagttgt tcgtttatca gcatctaaaa
    35041 acctcctttt actcattata gcgtatattt tttttagaga aaaacagata agaaaaggaa
    35101 ttgtcagatt ttttgaacta tttttttctg taatttgtgg tatcatgaac acgaagactt
    35161 aaataagtgg aggattaact ttatgacaat tgattggcaa aaagaagtag aagcacgtaa
    35221 agaagacttg cttgaagact tgaaaaatct tttacgtgtc aacagcgagc gcgatgactc
    35281 aaaagtaaca ccagatgcac catttggacc tggacctcgc gacgcattga aacatatgtt
    35341 agcttatggc gaacgtgatg gatttaaagt aaaaaatgtc gataactatg cagggcatat
    35401 tgatcttggc gaaggagacg aaacgctagg gatatttgga cacatggatg tcgttcctgc
    35461 aggagatggc tgggacacag atccttatga accagtaatc aaggatggaa agatctttgc
    35521 acgcggatca agcgatgaca aaggtccaag tatggcagct tactatgcga tgaagatcat
    35581 taaagaactt gacttgaaac tatcaaaaaa agttcgcttc gtagtcggca gcgatgaaga
    35641 aagcggctgg ggcgatatgg cttattattt cgaacatgaa gaagaaccag attttggttt
    35701 ctcaccagat gctgaattcc caatcatcaa tggtgaaaaa ggaaatgttt ctttagcttt
    35761 acgcttcaaa ggcgacaatg caggagatta tgtcttgaaa tcttttgttt ccggacttcg
    35821 cgaaaatatg gtgccaggaa cagcaactgc agcacttcaa gtaccaagtg cagatgcagc
    35881 tatcgcgatg gaagaagcat tttaccaatt catcgaagca aatccagtaa gcggaacgat
    35941 tgaagcggac aatacgtatg taaaaatcga attagttggt aaaggcgcac atggtgcaag
    36001 cccacaatct ggtatcaatg caggttcttt cttggcattg ttcttagata actatgaatt
    36061 cctaggatct gctaaacaat ttatccatgt agcagctgct tatgtccatg aagattttta
    36121 tggagaaaaa ctaggagtcg cttatgaaga tgaaaaaatg ggcaaattga caatgaatgc
    36181 tggactattt gcttttgaag aaaatggtac agaggaagcg aacttcatca atatgaattt
    36241 ccgttttcca aaaggtgtaa cagtagacgg attgcaaagt gacatcgaac aaacagtggg
    36301 gcaagaaggc gcaacagtta ctcgaggtgc tcgcgtaatg gaaccacatt acgtaccaat
    36361 ggatgatcca ttggtggcta cattattgca agtctatgaa gaccacactg gagaaaaagg
    36421 ctacgaacaa atcatcggtg gcggaactta cggacgtttg ttgaaacgtg gcgttgctta
    36481 tggagcgatg ttcccaggat atactgatac aatgcatcaa gcaaacgaat ttatgagtct
    36541 tgatgacttg ttcagagcaa cagcaatcta tgcagatgcc atctatcgtt tggcgaaata
    36601 attaattaaa aagaggatca agaattgcaa attatttttg cagtcttgat ccttttttta
    36661 ctttctactg tggtattcat gataaacatg atatagattc aattcatatt ctttcttagc
    36721 gttcgtgacg actgtatcca aaatcaaccc tgttactaat gaaagaagtg ctaaagtcat
    36781 caatccagta cttaaaatcg cagaagggat gcgtgtaata aagcttgtca gcatatattc
    36841 gcgaatcaca ggaatccccg tgaccaatcc aaataaaaag aagaagatgg tccaaatccc
    36901 aaagaaaagc aaaggcttat aatctttaaa cattttaata atcatcatga tcactttgaa
    36961 tccgtcagag aatgtattca attttgattc gctgccttca ggacgatcac gataatcgat
    37021 tgggatttca acgaacttga attttttatc taaagcatgg atcgtcaatt ccgtttcgat
    37081 ttgaaaacca gaactcataa taggaaaact tttgacaaag atacgattga atcctcgata
    37141 gccggtcatg acatcaagaa tttttgtttt atacattcta gtaattgtat ttctaacaag
    37201 gttatttcca aaatcgtgga aaggtctctt attctcatca aagtaggttc catttgatag
    37261 gcgatcacca atgaccatat ctgcttcacc agaacgtaat ttctcgagca aaccatgtac
    37321 agcttctgca ggataagtat catcgccatc taccattaaa taatagtcgg catcgatatc
    37381 aaagaacatt tggcggatca catttccttt tccttgacga ggttcttttt ttacgattgc
    37441 tccaccttga acggccaatt cgtatgttcg atcggtcgaa ttattatcat agacataaat
    37501 atctgcttct ggtaattctc gtttaaagtc tgcaatgact gttgaaattg ttgcttcctc
    37561 attgtaacaa ggaatcagaa cagcaatttt ttcttgttgt tccatttttc actctcctta
    37621 actatagaaa acaaaagcta aattaaaaat acctcataat caaaggaaat gctagttttt
    37681 cccgctaaat atcatttatt taaaaagaaa atactggaat atatgtttgg aaagctctat
    37741 ctaaaaaacc acccactaca aaagaaggac atgatacctc tcttttgaat agtgggtgcg
    37801 tattcgttct ctcacttttt tgacgtatta atctgttgca cctttttttc ttgaaatttc
    37861 taatacggtt cctgtatgag catctgctaa aaattcataa taaacagttt cgccatcttc
    37921 tattcgagaa agacctcccc gataagcact cgaccgaacg gcgaatttcc taataggttc
    37981 cttttcaaaa gagatccaag aaccttcgat tgcaccttct tttagaaaag atgacttgat
    38041 attttcaagt acttggtctg ctgacaatac ttttttgcga tgataccaaa tcgttgaagc
    38101 taccccgctc aataatccta gaccgattcc aactgctaac ccgcctttaa aatagcttag
    38161 ttcgctttct tcatacatgt ggacatacct ccttcttctc acttgttact ccttgtgctt
    38221 attttaccac actctcatat tttctttgaa ataaataacc gctaccagaa aaaataagct
    38281 ataataaata cgatcggtct gcagaaaaaa tcatatttta tccgcagatc aatataagtc
    38341 tgagaggagt ttagtctgaa atttagacga atagcatatg gaagaaaaaa catttcaacg
    38401 aatcaaagag ttaacagaat tacaaggaac aagcggtttt gaacaagatg ttcgcgctta
    38461 tatggaaagt catatggaac cgctggtaga tgagttacag ttagatggtt tgggcggcat
    38521 ttttggtctg cgccatcatg aagaagcgga tgcaccccgt gtaatggtcg cagcacatat
    38581 ggatgaagtc ggattcatgc tgacacaaat ccaagaaaac gggcttttta aagtcgtacc
    38641 tctaggcggc tggaatcctt acgttgtttc tgctcagcgt tttacactta aaacctctac
    38701 tggtaagaat tatccatgta tttcttcttc cgttccccca catttattgc gaggaacaag
    38761 cggacaaaaa tcagtagaag taaccgatat tttgttcgat gctgggtttg aatcaagaga
    38821 agaggcaatg agttacggtg tccttccagg agacacaatc gttccttacg ctgaaacgat
    38881 caaaacagcc aacggaaaga atattatcag taaatcatgg gacaaccgct atggctgtac
    38941 aatggtatta gaagctttag aagcgttgca aaatgaaaca ttaggtcata cattgattgc
    39001 aggcgcgaat gtccaggaag aagtaggctt gcgtggttct aaagcttctg tcaataaatt
    39061 caagccagat ctgttctttg cagtagactg ctctgcagca gatgatacag tgacgaaaaa
    39121 tggcacattc ggacatttag gagaaggtac attgatgcgt atccaagatc caggtttgat
    39181 catgcttcct cgtttgcgcg agtacttgct ggatatcgct gaaacaaaca atatccctta
    39241 tcaatacttt gtttcaaaag gcggtacaga tgcaggtgct gctcatacac aaaacgaagg
    39301 aatcccaagt acagtgattg gcgtagtggg acgttatatc catacgcatc aaacgatgtt
    39361 cagtattcgc gattttgaag ctgcacgaga aatgttgatt caaacactga aaggtttgga
    39421 taaatcaaca gtaaatacga tcgtatacgg aaaataatgc gatagtgaaa ctataaaatc
    39481 tggcaaaaga ttgctttttg ccagattttt tttataaaaa gaggatcaat gtttgttagt
    39541 tcgaaagaaa ggtgtataat ttaattgaaa aatacataaa cagaaagtag aggacaaaga
    39601 atgattatcc caaatagttt agaagaatta gcaggctacg tagaaactgg aaagagtgta
    39661 tttttcttta ctgccggctg gtgcggagac tgccgtttta tcaaaccgca aatgcctgaa
    39721 attgaaaatg acttttctga ttggcaattt attgaagtag accgtgacaa gtacatagat
    39781 gtagccgctg aatggaacat ttttggtatc ccaagttttg tcgtgatcca agacggaaaa
    39841 gaattaggtc gtctagtgaa taaagatcgt aaaacaaaac aggaaatcga atcgttccta
    39901 gaaagtgtaa caaaaggctg acatctgtta ggaggggtca agatgaatca agaatataag
    39961 aatattttag taggtatcga cggtagtgaa caagcacttg ctgctttcaa aaaagcggta
    40021 gaagtggctc gccgtaataa tgggaaagta tatgtagcaa acgtaatcga tcagcaattt
    40081 tataacttta tgggctatgc acctattgat caaaatctag tcgatcagca aactgaaaca
    40141 gccaaggaaa tgatcgaaga atgtaaagaa tatgggaaat ctgttgatta cacaaatata
    40201 gaaggaatta ttgcatatgg ttctgttcgt gaatccatgg cagtcaaatt gccaaaaaaa
    40261 tatgagatcg atctaatcat ggttgggcag tcaggtctaa atgcagtcga acgctttatg
    40321 acaggaagtg tagccagcta cgtgatacgt cattcacctt gtgatgtctt gatcgtttct
    40381 gacgaagaag aacagccaag taattaagca agtggaactt gtacgacctt tttaatcaat
    40441 taactaaaac agtaaaggga ctgtgacaaa agtcttgtca cagtcccttg ttacgaataa
    40501 atggtgttcc agaagaggct tcttcggaaa taaagcatga aaaaaatttc atgcagtgaa
    40561 tgcaattttt tgttaagata aatctcagca aaggataatg gaggaaagaa catgattttt
    40621 gcttataaca aagcacacgt cggagacacc ttgatggtta ttatagccga cgataaagga
    40681 acagaaaaca ctgttgaacg caaatggaat gttgctcgga tcaaagatga acaaggtcaa
    40741 gttgttgctt ggaacttttt ccatatctcc gatcatctaa caatcgaagg gaacggccaa
    40801 gtaagcttaa cagatgagga attagcgaaa ttgaatcgat tgatcaaaga agcaggattc
    40861 gaagaacagc ttgaagctga tcatgaaccg aagatcgttg tcgggtacgt aaaaacatgc
    40921 gtcccacatc ctgattctga ccatttatcg attactgaga cagaagtaga caatggtaca
    40981 gtattgcaaa tcgtttgcgg tgcacccaat atcgaagctg gacaaaaagt tgtcgttgca
    41041 aaaccaggag ctatgatgcc agatggaatg atgatctggc caggaacttt gcgaggggta
    41101 gatagctatg gaatgatctg ttccgctcgt gaattgcatt tgccaaatgc tccagcaaaa
    41161 aaaggaatct tagtattgcc tgaggatgcc gtagtcggag aagcatttcc aaaataaaga
    41221 ggttgtgaca agtctttgtc acaacctctt ttttattaat tttcactatt ttgattttgt
    41281 tttaatgccg attgatcgat cgtcaattca acagtagttg ttttttcttt cgagccttca
    41341 tagtaagtga ttttgacaga atctcccact tttttcttat acaaagcaga ttgaagttct
    41401 acaccagaag aaacttcttt gtcatcgatc ttagtaatga cgtcgtattg tttcaatcca
    41461 gctttttcag ccggtgtagc tgtttggaca tttgtcacga tgacaccgtt agttacagat
    41521 gaaggaatct tcaagatttg ttcttgttgc tgagtagaaa cggcagataa gtcgaccatt
    41581 gtgataccta gagctggacg tgtcactttt ccgtcttttt ccagttgatt gatgatattc
    41641 actacatcgt tacttggaat agcaaatccc attccttcta cgcttacatt agaagtagat
    41701 tcagaagtac ttgcgatctt acttgagttg ataccgataa cttgtccttc gatattaact
    41761 aaaggaccac cagaatttcc agggttgatt gctgcatctg tttggatcgc attgatattg
    41821 actgtttcgt tcgattcatt cgtacttgtc acttgtcggt tcaaggaaga aatgattcct
    41881 gaagtgacag aatttgcata ttcagatcct agtggggaac caatcgcaat cgcaggctca
    41941 ccaaccttca gagcacttga gtcaccaaaa gaggcaactg tttcgacttt gtctgaattg
    42001 atcttcaaca ccgctaaatc tgaataagca tctgttccaa ctaattcagc ttttaccttc
    42061 gtaccgtctt tcatcaagac ttcaagacct tgctggccat ctactacgtg attattcgtg
    42121 actacataag cagtattccc gtcttttttg tagatcacgc cgcttccttc actataagct
    42181 tccaaattac tatcatctga gctgctactt tcttcttgct gcccgaatag tccgccaaaa
    42241 ccgcttgatt ggttttgact ttgtaagttg ataacagaaa cgacagcgtc ttgaacttta
    42301 tctaccgctt tggtgatatc agaatctaca ttgactttga cattttccac aactgtttca
    42361 ccggcagaat tctgattgcc gcttgtagca gtagaactat tgttgccagt gcccatcgct
    42421 gcataaaaaa tgccagctgt cagtagtcct ccaacgatcc ctccaacaag ccccagacct
    42481 aacttacgcc agaggctatt atttttactt tttttcatct ttggtgtcac attttttcta
    42541 tccatgtgtt cctcctccaa ttacattcat ttatctactg ttagtatagt cttatttgtt
    42601 gactttagtc tactttgaga ctgtgaactt tttatgaaaa actttaggaa aaatcattgt
    42661 tttcagatga aaatatttga ttttcttttt ccacagatac tgtgtaaaac tacgcggata
    42721 ggattggaaa aataagggat aaaacatatc cacagcctgt gtataaagtg gataaattct
    42781 tataaaacgt atgtttgcct aatgaatgtg atgaaataag gtaaaataac actgttttct
    42841 gtggataaac ctgtggatgt tgtgcataac tgttggggaa atctttttaa caaaaatagc
    42901 caaaataaac atatagacga aaaaattgat tataataaac aaagtgagca tgaagaccaa
    42961 aggagagaaa acatgaatat caaaattatc tcagtaggaa aattaaaaga aaaatacctg
    43021 atccaaggta tcaatgagta tgtcaaacgg ctgaatgcct atgcaaaaat cgaattgatc
    43081 gaagttcctg acgaaaaagc cccggagaat ttaagcgaag cgcaaatgag acaagtaaaa
    43141 gaaaaagaag gggaacggat cttagcgaaa atcaaagaac aagaatacgt ctatgcctta
    43201 gcaattgaag ggaaaaatcc tacaagtgaa tcttttgcta aacaaatcga ccaattaggt
    43261 atccaaggaa aaagccacct tgtttttgtt atcggcggct ctttaggact cagcgaagcc
    43321 gtcatgaaaa gaagcaatgc tcagatctct tttggaaaaa tgacgtatcc acatcaattg
    43381 atgcgtctga tccttgtcga gcaaatctac cgtgccttcc ggatcaatgc gggggctcca
    43441 taccacaaat aatgatattt tttaagagaa aaaattatcc ctatttta
//
LOCUS       DACCYH010000016        37640 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.016, whole genome shotgun sequence.
ACCESSION   DACCYH010000016 DACCYH010000000
VERSION     DACCYH010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.016"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            25..702
                     /locus_tag="IVY85_000816"
     CDS             25..702
                     /locus_tag="IVY85_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8043850.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            705..1832
                     /locus_tag="IVY85_000817"
     CDS             705..1832
                     /locus_tag="IVY85_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8043851.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(2032..2472)
                     /locus_tag="IVY85_000818"
     CDS             complement(2032..2472)
                     /locus_tag="IVY85_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8043852.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(2483..4516)
                     /locus_tag="IVY85_000819"
     CDS             complement(2483..4516)
                     /locus_tag="IVY85_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8043853.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(4518..6221)
                     /locus_tag="IVY85_000820"
     CDS             complement(4518..6221)
                     /locus_tag="IVY85_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8043854.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(6315..7265)
                     /gene="manA"
                     /locus_tag="IVY85_000821"
     CDS             complement(6315..7265)
                     /gene="manA"
                     /locus_tag="IVY85_000821"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP8043855.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(7304..8329)
                     /locus_tag="IVY85_000822"
     CDS             complement(7304..8329)
                     /locus_tag="IVY85_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8043856.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(8538..9407)
                     /locus_tag="IVY85_000823"
     CDS             complement(8538..9407)
                     /locus_tag="IVY85_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP8043857.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(10111..12003)
                     /locus_tag="IVY85_000824"
     CDS             complement(10111..12003)
                     /locus_tag="IVY85_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8043858.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(12000..13727)
                     /locus_tag="IVY85_000825"
     CDS             complement(12000..13727)
                     /locus_tag="IVY85_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8043859.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(13743..14393)
                     /locus_tag="IVY85_000826"
     CDS             complement(13743..14393)
                     /locus_tag="IVY85_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8043860.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            14597..14893
                     /locus_tag="IVY85_000827"
     CDS             14597..14893
                     /locus_tag="IVY85_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8043861.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            15085..15705
                     /locus_tag="IVY85_000828"
     CDS             15085..15705
                     /locus_tag="IVY85_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP8043862.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(15797..16963)
                     /locus_tag="IVY85_000829"
     CDS             complement(15797..16963)
                     /locus_tag="IVY85_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8043863.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(16975..17328)
                     /locus_tag="IVY85_000830"
     CDS             complement(16975..17328)
                     /locus_tag="IVY85_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8043864.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            17456..18478
                     /locus_tag="IVY85_000831"
     CDS             17456..18478
                     /locus_tag="IVY85_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP8043865.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(18917..19351)
                     /locus_tag="IVY85_000832"
     CDS             complement(18917..19351)
                     /locus_tag="IVY85_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043866.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(19461..21320)
                     /locus_tag="IVY85_000833"
     CDS             complement(19461..21320)
                     /locus_tag="IVY85_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8043867.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(21438..22598)
                     /locus_tag="IVY85_000834"
     CDS             complement(21438..22598)
                     /locus_tag="IVY85_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP8043868.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(22905..24329)
                     /gene="araA"
                     /locus_tag="IVY85_000835"
     CDS             complement(22905..24329)
                     /gene="araA"
                     /locus_tag="IVY85_000835"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP8043869.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(24345..25043)
                     /locus_tag="IVY85_000836"
     CDS             complement(24345..25043)
                     /locus_tag="IVY85_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8043870.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(25000..26661)
                     /locus_tag="IVY85_000837"
     CDS             complement(25000..26661)
                     /locus_tag="IVY85_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP8043871.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            26879..28279
                     /locus_tag="IVY85_000838"
     CDS             26879..28279
                     /locus_tag="IVY85_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP8043872.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(28531..29610)
                     /locus_tag="IVY85_000839"
     CDS             complement(28531..29610)
                     /locus_tag="IVY85_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8043873.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(29696..30655)
                     /locus_tag="IVY85_000840"
     CDS             complement(29696..30655)
                     /locus_tag="IVY85_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP8043874.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSKKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(30667..32190)
                     /locus_tag="IVY85_000841"
     CDS             complement(30667..32190)
                     /locus_tag="IVY85_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8043875.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(32200..32763)
                     /locus_tag="IVY85_000842"
     CDS             complement(32200..32763)
                     /locus_tag="IVY85_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP8043876.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(32769..33632)
                     /locus_tag="IVY85_000843"
     CDS             complement(32769..33632)
                     /locus_tag="IVY85_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8043877.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(33646..34542)
                     /locus_tag="IVY85_000844"
     CDS             complement(33646..34542)
                     /locus_tag="IVY85_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8043878.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(34717..35988)
                     /locus_tag="IVY85_000845"
     CDS             complement(34717..35988)
                     /locus_tag="IVY85_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8043879.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(35978..37495)
                     /locus_tag="IVY85_000846"
     CDS             complement(35978..37495)
                     /locus_tag="IVY85_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8043880.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT        10923 a         7850 c         6521 g        12346 t
ORIGIN      
        1 aaattttata ttggaggcct atctatgaca ttactgatca aaaacatgaa catgaaacgt
       61 ggtaaaaaac gtgtgattcg acaattgaat cttttagtag aatcaggaga agtagctgct
      121 ttgattgccc cgaacggatt tggcaaaaca actttgctga atggcattgc ccaactgctg
      181 ccaacaactt atgatcaatt gtcccttcac aacatctctc ctactgctta tacagattat
      241 cgccggtctt tcttttttct agaatcaacg aatcagttat ccgactatct atcggctgtt
      301 gattatttga ctttgatcaa atactattgg cagagcaaag tagaatgcca aactgttttc
      361 caacgattga agatgactga gtaccttact ttgccgattg gaaaaatgtc tctaggaatg
      421 aaacaacatg tcttgcttgc tgcttacttg atcagtgatt catctgtcat gttattcgat
      481 gaaccgttga acggattaga cccaggaagt attgatatct tgaatagcct catcagacaa
      541 tggcagaaag aggggaagac gattcttctt tcttcccacg ctatcgcgaa tatccaatcg
      601 atttgttcta aagcatgttt tctaaaaaat ggagaaatcg tcacagaatc ctcaaatatg
      661 gaagagattt tagctattta tcgtaaatta tatgaggtgt aactatgtct ttacttacat
      721 ttgaacgcaa tctcttacta aaaaacaaag taaacttttt atttcctcta ttgttggtcg
      781 tgctctttgc attcccactt ttttttgatc ataaacttgc ttatacagaa ttcgatgaac
      841 tgaatcataa ctatgaagag atgcaacgtt tgattgaaac gttgaaagaa gatgagaatg
      901 agaaagaatt tgtggaaagt ttagagaaaa gcaacaaact gatcgaagca atcttacatg
      961 caaaaaacac agggaatgtc cagcaaatgg tcgaagcaac ttatcattat gaaaaagata
     1021 tactggatcg actgatttct ggtcaaagac aaggaattcc aatcattgaa caacaaaaac
     1081 gtgtcgagtt gctccgttat atgaaagaac atcagatcca aagatattct atttttgact
     1141 tgccagcgca tctctcttta gcaaattact acgaaaatat tttttcagga atgatttcta
     1201 gttttttgat tttatgtata accgccttgt ttctttcttc catcatctct tatgaaaaaa
     1261 gaaagcaggt aatctctcta gtcaatctat tactggatag tatggtaaag aaacatagca
     1321 tccgttttac catttattat ggtgcagcta tgctaagtct cgtgatgcca tttttgatcg
     1381 tctctatact agtaataatc aaaaatggtt tgggagactt ccgttatcct gtaggaacga
     1441 tcattggtca ggagatacga atcctgccga tgtatgagta tcttttccag agctttctgt
     1501 tcctgttgtt gtgggtgctc tttttaagta caatcagttt tcttctatct gctttatttg
     1561 aacattctct agtcaatctt ttaggtacac ttctttgtct tttcttagca gagtatcgct
     1621 tgttttcttc gattggatgg atagaatcaa tcagtcatta cttgccaact tcttatgttg
     1681 attttcaaaa tgtgatcata ggaggagata ttttctcgcc actagcttca gaacaggtta
     1741 ctttcatgaa tggtatcttg acacttggta tatggagtat cgttttatta ttcataggta
     1801 tgggcactat ctatataaaa aagagctatt aaaacacaaa aataataggg gcggtgacaa
     1861 aaaccttgtc accgtcccct atttcaaata aacggttttc tagaataaat agttttcaaa
     1921 agcttgcatc cgcagtaata agaacctccc aacaaaaata gctcctctta tttttatctt
     1981 ggttgaagtt attacttgat gcaaggcagc tttttttact gtgaacattg gttatatatg
     2041 tccatgttca aaaatcattt ggacttcttc aatggtttcc gcatcactga gcgccattac
     2101 ttggtcgatg tctgaacaga aaaaggctaa atcttggagc accgctaact gatgttcttt
     2161 ttttaaagcc actacaaata agagtgtcgc gatttttggt tcctcttttg ttccaaagtc
     2221 cacaccatca ggcacttgga ctaaatagat tccttcttct actacttctt catctttgtc
     2281 agtcccatgt ggcaaggcta cgaaattccc gatatagaca ctcgcttttt cttgtctttc
     2341 gatcattgat tggatatatg ccggtttgat tcccccttgt tgggtcatcc actggccgat
     2401 ctcggtcaac gcttctttcc aattttgata ggattgatcg agcaatattt tttctgctgg
     2461 tagctgtatc atcttttcac cttcatttct ttatttcttc aatcaggatt ttcgctaaca
     2521 actgataaac gatttcttta tttcctgagc ggaagatctc ggtgttcaag tcattcatga
     2581 tgattgctcc actgatcttt cctaaaagaa tcccgtcttt ttcttggatt ggcgaaggcg
     2641 ccagcataac taacatacgg gacaaatcga ctggctgtct atccatccct tcaatggtca
     2701 acggatgttt caaattgaaa atacaaaaga tcggtttaga aatcaatgag ctggatgtat
     2761 ggaataaccc catatttgta tgaggaatac cgataggtgc ttgttcatat cgcttaagca
     2821 gccggcgatg aaccacctct tcatccgtta ctagtccttc aggaaaataa ggaaataagc
     2881 tgcgaatcgt ttcggaaatc gtctcaggat tatccagttc ttggataaaa aaacgaccga
     2941 gtaattgatt gatttgatcc atgaaatgca ttgtttcctc ataagtatct tctatcatcc
     3001 actcgttatt gctgttatac gtaatttttt gccttttttt cttctcaata tgcgaaaaag
     3061 cttcttttag ctgcttgact tcatcttcta gcagtagcgg ggagactaaa atatattttc
     3121 caccgtatcc tggtaaaata gatgtagaaa taacaacatc atattcttct tgtaaatcga
     3181 ttttttttaa atcagcaatc cgatagaaat gaatttgttg gatgaaggga aaataccttt
     3241 tcaatcgtag ttccaacatg ctagtagaag ctaatccact tggcgaaaat cctgcaatac
     3301 tgatattgct ttctttcgga cttcgttcca atgaattcgc aaaatgaagg accatataag
     3361 cgatctcttc ttcggataat tttttttctt gaaaaatccg ctctactgct ttacggattg
     3421 cattcgcaac ttcctcgtat tgaaccatga ttttttctaa aatcggattg gtcagtgtct
     3481 cttcttgtaa aacggctctt gaaaacacac cagataaatg agtcaacagc attttgtaca
     3541 aagtttcatc ttcataaaaa tggacattcg tctcttcact tacttcttga atcagttgtt
     3601 ttactaaata agcaagctgt gtatcgaacg tctcttcaaa gaaattctga tcaaatgagt
     3661 tcgtgaagtc atttaataat cctgcaaaaa agacgatctc atttactgga taaagttgtt
     3721 ttgtctcaga agcgatccgt gaaaacaatc gtttagctgt ctgtagtaat tctttgtttg
     3781 ccagactatt atatgtctca tttgtcagta aatgattttt cgtcacacgc tccatcgtca
     3841 atgtcagcat caaaaccaaa tgttcaagct ttctatcact tagattggtc tttttttgga
     3901 tcaattcttc cccgattact ttgcgtgcaa gagatagatg ttgcggatca acgaatcgaa
     3961 aaaaataatt cagtgatgca tcgaaagaaa tactatgaaa aagttcatat tcattgattt
     4021 ctaattctaa tacattcgct gtcagaagcc ggcggtattt ttccggtcct tcgatctcgt
     4081 atccctggtt ccgaatgatt tccaaaggca gttggcgaat actttcttcc aactgtttga
     4141 tatctgcata aaaagtggta ttactgatgc gatatgattc catgaaatga gcaagactca
     4201 acggctctgt cgtcagtaac aactcggcta aaatcgcgtg ttgtcgttta gcagtggata
     4261 agtcattact tttcccatct gtattttctt ttaattgctc taatacttct tctgatgcct
     4321 caatgacaaa tgagcctcta gctgcttttt tcaaagaagc atttaccgat ttcaatgaga
     4381 cttcaagatt atctaattcg cggtaaatag tccgtttact gaccttcaat aaattcatca
     4441 ttttttggat agatacaggt gacgggctac tgagaaattc tgctaataat tgtttttctc
     4501 tgggcgataa atacatctta tatttctccc ttcacgagtt tttcgtattc attcgtagct
     4561 acccaatgtt caactagaac tagccgaggg atcttttctt ctagctgttc agttacagct
     4621 agttctcttt gtgcgatata aatcgtttct tcttggattt tgattttttc tattgattct
     4681 tttgagataa caaactgttt tttcgctttt tttgctaatt gacttaaaat cgtcatgccc
     4741 atagtttgtg agccgcgtct gttgttttta tacaaaaata caatattagc aggcgacgtt
     4801 ttttgttctg tgatatctgc tatctctttt ttttcttccc gctttggaaa ttggatcgct
     4861 tcttttattc gtgacagcaa gatatctgtt tccaaaaagt gatccagtac atagatttga
     4921 ctttgaggta ctttttctct tgcaagaccc gctagttcat gttgcacgat aaccagctga
     4981 tttacttggt tattcaattc atagatagag ctgtatccga cagaaagatc gattttttct
     5041 gctgctaatt tctttttcag cagggcggct cccatagcac ttgatcccat acctgagtcg
     5101 caagcaaaaa tgacggaatg aatgatagat ggttccaaga tttctcccct ttctttgacc
     5161 gttttgttct ctttttcttt atttggtaat ttcaatagta tcgctgcaca aatgaaggtc
     5221 acaaatgtac tgattgctat ccctatactt accccagcta gttgtggata aggcgcattg
     5281 gcaaaaatca cgagtagtga cccaggcgat acaggtgcag ctaatcccgt atctaaccat
     5341 tgaaatatca acgtgccgct tgccccacca gccattacag ctaaaaatag atacggtcgc
     5401 agcaagacaa atggaaaata gatttcgtga atcccgccaa tcgcatggat catcaatgct
     5461 ccactcgcat ttactttttc ctgtttttct gcaaaacaaa ggatagaaag taagatccct
     5521 aaaccaggtc caggatttgc ctccatcaaa aataaggaag agtacccgac tgtttgtgag
     5581 aattcgattc ctaaaggtgt caatacacca tgattgattg cattattgaa aaatagtact
     5641 tttagaggtt ctaagatcag actgacaagt ggtaacagat tcattgtgac tagccaagac
     5701 acaacggagg cgagcaacaa gtgaacacta tttaaaactg gttcaataaa aacgattcca
     5761 attatacaaa taacgctacc agtcagtcct actagtaggt tgctgacaag catttcatat
     5821 ccagccttga atttcacatg gaatctagaa acaaattgtt catatacgat gccggcaagc
     5881 ggcccgataa tcatcgctcc tacgatttgt ggagcattgg aacgagcgat caaccctaaa
     5941 gaagcaattg caccggcgac tgcccctttt tgtcccccta ccgatttccc gcctgaataa
     6001 ctgatcaaga tgggtaaaag cagctggatc atccaatggc atacctcttc taatgtggtg
     6061 tgcaaatcgc cagacacacc ttttgttgct aatgacaaga ttccccaagc gataaacaga
     6121 cttagatttg gcatgatcat ttcatttaag tgatgtccag cttttctcag ttgtttcata
     6181 gacacgcgac tgctgttgtt tgctttccat ttgtattcca tctttttcac cagacttcta
     6241 tttattgatg gacggatttt atgtagaaaa agcagcaaac gaaaaaccgt ctgctgcttt
     6301 cagctatctg ggaactattt gcctggttca gacgcaatga ttgctagttc accttggacc
     6361 gcccactcag aaacgccatt tggaataata cagcttgttc ctttttctaa tggatatgtt
     6421 tttccgtcaa cgacaaggtc tcctgctcct tcgattaccg tcatcaatgt ataaggggct
     6481 tgttttttga aagatgtgat tcctttaata tcccactcat atacattgaa aaactcagtt
     6541 tctaaataag tgacgatcga tgaattccct tttcggactt ctttgatttg taattctggt
     6601 gttttcgcag ggacagttgt tacatcgatc gattgttgga tatgcagctc tcttgtttgt
     6661 ccttgcgcat cttttctgtc gtagtcatac acgcggtaag tcgtgtcaga actttgctgt
     6721 gtttctaaaa tcatgattcc tttaccaata gcatggatcg ttccgcttgg tacataaaag
     6781 aaatcacctt ttttaactgg tactttcttc aaaaggtcat cccagcgccc atctttgatc
     6841 atctcagcta gttcttctct tgtctttgca tgatgaccat agatgatttc tgcaccaggt
     6901 tcagcatcga tgatatacca gcattcagtt tttcctaatt caccttcatg tttcatccca
     6961 taggcatcat ctggatgtac ttgtacagat agatcatctt ctgcgtctaa gatcttgatc
     7021 aataatggaa acactggttc tgaaggatgg ccaaacaatt ccttgtgttc tgaccatagt
     7081 tcatctaatc ttttcccttt gaattcgcca ttttctacta cactgacacc atgaggatgc
     7141 gcactgatcg cccaatcttc tccgatttta tcgttaggga tatcaaaacc aaagactgaa
     7201 cgtaaacgac ttcctcccca aattttttct tgaaaaactg gttttaaaaa cataggttcc
     7261 tgcataattc aaactcctct catttttcat agctattata gcattatttc ggtaataccg
     7321 ctttctcttt caaaaagttt tcaacaattt catctcgaca tatgagatac tccgcacgat
     7381 gatcgacagg atgtacgcga tttgacaaaa atatcaacgc ttcttgtgtt tttatatcaa
     7441 tcaagaaaaa cgtacctgta taaccagtat gaaaaagaat cgcatgaccg tcttctacat
     7501 ccttttttaa gtcccatcct aaagaacgat agccattttt tttcggtgtt tgatcttgca
     7561 gtaaagaatc aatcgtctct tcttctaaaa aacgaccatt acggattttt cccttgttca
     7621 aatacatttg gacaaaagta atgctgtctt tcaaatcaat aaagagaccc gcgttacctg
     7681 catgttcagc caaaactctt gctttcggat catgagtttg tccacgcaaa acctcacccg
     7741 aaggcagttg ttctgtggga acgatttttt tggaaggaaa aggaagaaat aagctgttca
     7801 tcattcctaa tggttccaat acttgttctt gaaagatttc cactgcagtt tcttggtaaa
     7861 tttcctctag catcaaccca agaagaatcg taccggcatc tgtatatttt acttcttttc
     7921 ctagttgctc acctggttct agcttcaaat aagcggcgat caattcttcc tgagatagct
     7981 tatctctgtt tggtatccat gttttgatat cagaagtatg tgtcagtaaa tgacgcaaag
     8041 tgattgtctg attttcaaat tcaggaagat atttatgaag cggttgatcg agttctatct
     8101 ttccatcttc taatagacgt aaaactaatg aagtggttgc aacgactttc gtcagtgagg
     8161 ctgcatcaaa caacagatct tctgtcatca gctgcttttc aggaacgact tgggcaaagc
     8221 cggctgtaaa tgtttctact tgattttctt ttatgaaggc gactgctaca cctggataaa
     8281 atccttcttc catctttttc aaaataagct gtttcgtgtg tggatacatc tttttctcct
     8341 gcttttctat cagattctta cagtccaagc ttaaagctta atctatgagg aatcaacctt
     8401 ttttacacaa aaaaactgcg cgaaaaaccg cgcagtttcc cccggtatac tattttctct
     8461 ttctatctct agaataagcg atagtatgac cgttcataaa atgacgagac atgcgacaat
     8521 cgttaagaaa gaaaaagtta agcgtcgcat cgaatgtcag ttttattctt ttcttttggc
     8581 tcactgacga accaccattc gattcctata gcatcataga ttgtgagtac tgtgtgcttt
     8641 ttgtctaaga agaaatcaac tgatagttgc tgcaagtgag caatcagtcc ttccatgctt
     8701 tcagtatcga tcatgaattt cagaaaatca agtccaatca cttcatctgt aggcaaatca
     8761 attgcgcctc cgctagcttc tgtcaaacca acatggaaat ctccttcatt caatacatgg
     8821 atgccctctt cttcattatg taccttcaaa ccaagtacat gattaagaaa ttggcgttct
     8881 tttgttaagt tagtaacatt taaatggatt ttttcaaaat aagaacctgt tgatagttta
     8941 ctgaattttt cttcagattt tcttaataat gcttcttgat ccattgtttc tggaatttct
     9001 ctttggtcgt tcttattctc ttcatggtaa atttccaatt cattctcttc tgggtctacg
     9061 agcaaaatcc ccatacgtcc atgatcttct aaagcttctt tgattggata attcatttta
     9121 cggatacgac aaaggatatc tgccatttct tctgtgctag ggataatcaa tgaaaaacgg
     9181 ttcagctttt tgatcacccc catgtgatca ttcgctctag gactttcttc cagcagcaac
     9241 atttcacttc cagcttcttc tgttcctaaa atggctaatt cgttttcttc tcttttcaaa
     9301 tcaaagccaa caacgttctt gtaaaaatca atcattttat ctcggtcttt cacccgaatg
     9361 gctacacttt ttaaataggt ggcagccccc aattgaaaat ctttcatatt tcttgctccc
     9421 ctctacgtca agctttattt taacgatgga ttcttcttct ttcaagcaat aactttgatt
     9481 ttgttaaaaa aaatgtgaga aaattcgcat tttgcttgtt ttttgtaaat taatgagtaa
     9541 aaactcttat aaaaagaggt tgtgaaaaag ctgttctgca tcaagtaata agaacagcca
     9601 aacaaaaata gctcctccta tttttcgttt ggttgggctt attaccgcag atgcaagctt
     9661 ttgaacaccg tttaaggaag aggttgtgaa aaagtcgctt agtttcgagt agtaagaaaa
     9721 attttgtgaa aatggctttc tacatttttg cataaatttg gcttaatacc gagaaactgg
     9781 cttttgaaca ccgtttacca agaggttgtg agcctgccgt ttaactccga gtcacaagga
     9841 gcaatttcaa aaaacagctt ctcaggtttg ttgactattg tgacttgtga ccgaggagtt
     9901 ggcaggagaa caccgtttaa ggaagaggtt gtaaaatcag cggtttgacc tgagtattag
     9961 agatacaaaa cggaaaaata gcttctcata ttttgccgtt ttttgagcta ataccgaagg
    10021 acagctgatt gaacaccgtt tattcggaaa aagggactgt gacacgactt ttgtcacagc
    10081 ccctaaaaaa aataataaat ttgtactatt ctatgaagct tcttcttcag aaaactgact
    10141 gttataaaga tctgcataga atccgtcttt ttccatcaat gtatcgtggt ttcctgtttc
    10201 tacgatcgaa ccatgattca tcacaataat gttgtctgca tcgcgtattg tagacaagcg
    10261 gtgagcaaca acgaaacttg tccggttttc caataggcgg ttcatcgctt tttgaatcag
    10321 tatttctgtt cgtgtatcta cactagatgt tgcttcatca aggatcagga catctggatt
    10381 tgctaagaat gcccgcgcaa ttgtgattag ctgacgctgc ccttgcgaaa tattgcttgc
    10441 atcctcgttt aatacagtgt tatatccttc tggcaattta cgaacaaaat cgtctacgtg
    10501 agcagcttta gcagcctcaa tgatttgttc gtctgttgca tcgtcgttcc catatttgat
    10561 attgtcatag atacttcctg taaacagcca agtatcttgc agtaccattg aaaactgtgc
    10621 gcgcaattca tcacgtgaaa gatcacgggt atctacacca tcgtaacgaa tactacctcc
    10681 agaaacatcg tagaaccgtt caagaaggtt gatcagtgtc gtcttccctg caccagtcgg
    10741 acctacaata gcgaccatct caccaggttt gacatccaag ttaaaatcag tcattagcaa
    10801 gttatctggt gtgtagccga attgaacatt ttcaaaacga actttgtatg gtgtatcttt
    10861 ttcttctgga aggccagatt tttcattgga catctcttct tcatccaaca cttcaaatac
    10921 ccgttcggct gaagcaactg ttgattggat agtgttcatc aaacttgcaa gttgagtgat
    10981 tggttgagag aactgtgttg tatattgcaa gaacgcttga acatccccta atgtcatgtt
    11041 cccatttgca acttttactc cgcctagtac agcgacgaac acatagccta gatttttaac
    11101 gaaattcatc aacggcatga tgattgccga aataaattga gctttccaac cagcggcata
    11161 cagattctga ttttcttttt caaatacttc ctggtctttt tcagtatgat tgaatgtttt
    11221 aacaatggta tgaccaccat atgtttcttc tacttggtta ttcaataaac ctaaactttt
    11281 ttgctgatct gcgaaaaaca cttgtgattt cggcgctacg atcatcacga tgatcaagct
    11341 taaagggatc atcaataacg cgactaacgt caattgccag ctgattgaaa gcatcatcac
    11401 gatgacccca atcaatgtga ctgcactagt tacaaattga gtcaaatttt gctgcagcgt
    11461 gcttgcgatg ttatccatat cattgattgc tcgagacatg atatccccat ttgtatgtgt
    11521 atcataatag gaaatcggta cacgattcat tttggcttcc aaatcatgac gtaattcata
    11581 aactgttcgt tgggaaacac gtgtcataat aaattgctgt aaaaagttga atacagcaga
    11641 aatcaaatac atcacgatta ctgtcgcaat gatctgcgcg attttatcaa agtctatcgg
    11701 aaattttgtt acttgttggc cttgttgcat ctgttgagcg ccagccatta cacctttgaa
    11761 tatttctgtg gttgctttcc ccaagacctt tggtgtttgg atttggaaga tcacggctgc
    11821 tattgctagt acaaatacag cgatgatcgc aaccatacgt ttagacatat agccaaacaa
    11881 tcggcgtacc gttccccaga aatttttagg cttttcacct tttacaccga gatttctgcc
    11941 aggtccacca tgtcctggac cgcctgctgg tccatgagag ggacgtgtgt ttgttttact
    12001 catgctaagt cctcctctct tagttgtgaa tgaacgattt cttgataagt ttcgtttgtt
    12061 gctaatagtt cttcatgtgt tcctttgcca accagttttc cttcatctag cacaaggatc
    12121 atatctgcat ccattactgt acttactcgt tgagcaacta aaacggtgat actttctttc
    12181 atattcgttt tcaatgcttg gcgaagattg gcatccgttt tgaaatccaa cgctgagaag
    12241 gaatcatcaa acacatagat atctgctgtt ttgactaatg cccgtgcaat tgccagacgc
    12301 tgtctttgtc caccagagaa gtttcctcct ccttgttcca catggctatc taagccatct
    12361 tccatctctg aaacaaaatc tttagcttgt gcgatttcta atgctttcca aatcatttca
    12421 tctgttgcat ccggcttacc atactgcata ttgtctcgga tcgttccaga gaacagaact
    12481 gctttttgag gaacaaagcc cattaattct cgcaaattat attgagtcat gtcactaatg
    12541 tccgttccat ttatttggat cgaaccagat tcgatgtcat ataatcgagg gatcaaattg
    12601 actaaggtcg ttttacctga accagtcccc cctatgattg cgacgatctc gcccgacttg
    12661 gcttggaaat caatatcttc caatgcaagt ttttctgctc catgatatcg ataattgaca
    12721 tgattgaagg ccaatgttgc gttttttcca gcaaaagaaa ctgttttagg attttcaggg
    12781 tcatggatac cgattttttc atccaacact tcattaatac ggtcagctga agcttgtgca
    12841 cgaggcacca tgataaaaat agcggaaagc atcataaagc tgatcaagat ctgcatcgca
    12901 taagtcataa atgcaatcag attaccgact tccaaagtca tatctgctat gtagtgtcca
    12961 ccaaaccaag tgattgctac gtttgtaccg ctcatgatca atgtcatcat tggcatcatc
    13021 aatgcgacga tcgtattaac tttgattgcc gtatttgtat aatctttatt cgctaaatca
    13081 aaacgatttt cttcaaagcg tgtcttatcg aatgctcgaa tcactctgac ccctgtcaat
    13141 ccttctcgaa ataccaggtt taaacggtca gttttctttt gcatactttt gaataatgga
    13201 acagcaaagt acatgattcc gccgatcaaa ataatcatta ctgggatgac aactaagaag
    13261 atttttgtca actgatgatc tttttgataa gcaagtatgc tcgcgccaat caacgtgatt
    13321 ggtgcattga tcatcatacg taaaaacatt tgtgtaacca tttggatttg agtcacatca
    13381 ttggtcgttc ttgtgatcaa agaggccgtt ccatatttat caaacgcatc ttttgtcaga
    13441 ctctctgatt ttttatagac atccgtacgc aattgcttac ccagtttctg tgattcacga
    13501 gtagcaaaaa atgtatttgc gatagcagct aagatactga tcaaagaaaa gccgatcatc
    13561 acaaaacctg tatgccaaat ataatcgaca tctccttgcg ctaccccttt atcaataata
    13621 ttggaggtaa gtgtcggcaa atataaatct gccagtactt ggatcaccat gaaaactgca
    13681 gctgcaattg ctgaagtcaa ggagatccgt tttacgattt ttagcatcta gttcccttct
    13741 ttctatttat atactccgtt tttcagccaa gaaagtttca aattgaattc actgattgct
    13801 tgctctactg agtaatcttc agatgttgaa aataagcgat acgaatgcgc aattgttgta
    13861 aatacagtat tcatcactaa ttggatcagt aattccaatt catgatcatt ttttactttc
    13921 agcttcgtcc gatcgatcaa ttcaagcaac tcttttgcat cttgtttgtg ttctttatgt
    13981 ggatcgtgag gcgttttttg gcgactcgta tgaggagcga cttttcttga ggcacggtaa
    14041 tccatgttca taaacaagtt acgataaaaa gaagcattct cgccttccaa aatctcataa
    14101 atcattttag aaaaatagga ttcgaatcct ttgaatagat caccatcgga ttctttcatt
    14161 agttgtaaca tatctttaga gctgtcacgt ctcattgtct caaagtaata aaaataaaga
    14221 tcctctttat cttcaaaata ctgatagaaa ctgcctctag ggatatccgc taatttgacg
    14281 atattagcaa tcgaagcttc ttttaagggt actctagaaa actcgattct cgcagcctcg
    14341 attagtcttt tttgtttttc tttaggcaaa tggaaaaatg tctgtgtcgg catatggatt
    14401 ctcctttctt tcaagatgac acattgtcat atgacgctat gtcattataa cacctcctac
    14461 ctgttttgca agacatttgc atagtttttt tcattatcat ccgatacatt aaaaaagtga
    14521 taagaggaat ccttccattt gaattaatga gagttgccca ttaaaatagc cacatactaa
    14581 acaaaggatg agataaatgg cccaatcaat tagtgtccaa gacttcaata aattgcctct
    14641 cgatgctgac agcatcgttt tagatacaag aaatacctta gattacacaa aaaaccactt
    14701 aagccactcg ctttctatcc ccgctgctat cttgccaaga caattaaaaa atctagatcc
    14761 aaagaaactt tattatattc taagctatac aggcaggcga tcagaagcat tagccgatca
    14821 acttactgct aatggttttc gtgctgttta tatcattggt gggatggaag ctcttcagtc
    14881 gagtgttgct tgaatattgc ttgttagatt caaaagaggc tgtgacaaaa aatcgtgtca
    14941 cagcctcttt tataccctgt ctttttttag ttatttattc ttcaacctta tcaatatgac
    15001 ttgatagcct tttctttttc gagtaaactg attttgttag tcatagttat aacaatcata
    15061 gcaataaaag gagaaggtaa gcatatggca tctgttttag tagtcaaagg tcatccccta
    15121 actgcagaag aatcgcgcac agttaaggca ttgacgtctt ttttaacaag ttataaagaa
    15181 aatcatccag atgatgaagt gactgttttg gaactttatc gcgatgatat tccggaaatc
    15241 gatgaagaat tattatcagg ttgggaagca ttacgtgctg gtgcagaatt cacttcctta
    15301 tcagaaagtc aacaacaaaa aattgctcgt ttcaatgaat tgactgatca gttccttgct
    15361 gctgataaaa tcgttatcgc aaacgcctta tggaatctaa atatcccaac aaaattaaaa
    15421 gcttggttcg ataccgtcaa tgtagctgga aaaacgttcc gctatacaga aaacgggcca
    15481 gaaggattag tgacaggtaa aaaagcactg cacatccaat ctaatggcgg tgtatacaac
    15541 ggacaagact ttgcctctca atatgtaaaa ggtattttaa actttgtagg catcgatcaa
    15601 gtagatcagc tattcatcga aggaatcgat tatgaccctg accgtgcaga tgagttgatg
    15661 caaaatgcat tagataaagc tgctgcttta ggtaaatcat tttaaaaatt gagataaatg
    15721 catacaaaag agacagagga aaaaatcttc aaagcagcat ttgtgccttg gatattttcc
    15781 catctgtctc tttttgttat tctttctgct ttttcaacgg attttcttca taaagagaag
    15841 agattgttgt gtaccattca aaaacatgag taatcactac tttagctgct gcataaacag
    15901 gaatccccaa gatcacaccg atgattccga acaatttccc agaagtcagc agaactaata
    15961 aaatcgttac tggatggatt gctaactgat tccctagtac taatggagag atcaagcgtc
    16021 cttctattgt ctgctctact gcaaaaacga tcagtacttt gatgatcatg aatggacctg
    16081 ctacaatggc taaaaagata gccggaatca tcgctaaaaa tgatcctaag taagggatca
    16141 ggtttaaaaa acccgcaata atccccaacg tgacagcata ctctaaacca atgaccgcaa
    16201 aaccaatcat aaacatgatt gccacagcaa aagccacagt tagttgtcct cttatataag
    16261 aagaaacttg actattcatt tcactcagca cattcaacgt cggtttttgc atgcgtgtcg
    16321 gtaaaaattt gacaaaataa ggtgctaatt gtttaccgtc tttcaataga taaaataaga
    16381 taaacggcat cgtcagcaga gccactaaaa tagtcgctac cgcgccgaca aaacttccaa
    16441 gactctgaac tgtactctta gatatatctt ggatcatatc gcctgccgaa ttcatgaact
    16501 tatcacctgc tgtttccaac tgttcgcgaa attggttgaa cagcgggtcc cgcaaaatct
    16561 cggttaattt attatcaatc gtatctacat attgagggaa gttactgatg aagctcactg
    16621 tttgttcctg aatcttaggg atgatcacta cgctccccca tgcgattagt gcaacaacca
    16681 aaacaaacag cccaatgata ctgtaaagcc ttggtacttt tcgcttttca aagtaatcaa
    16741 ctaccggatt catcaagtaa tacaagattc ctgctaaaat gataggcaga cctacgattg
    16801 ctaaaaattg ccagattggt gcaaacaaat atgaaacttt tgtaaataaa aagacgatca
    16861 gcaatattaa caatacaaca agtaaggctg tgactacttg attatttaaa aaccagcgcc
    16921 aaaaccacga taccggtctg ttcttcttat tttttccttc cataattcct cctgttagtt
    16981 ataaaaaatt tggcttccca aaccaatagc aggcagtcta tgcggatgaa gatagattcc
    17041 atttgcaatt tgtccttctt ctggacaagc atcaaaaatc aatgatacgt gagctactgt
    17101 ctttaaattc ccattggcca tcggaccgac ataatcgatc gtatatacct gatcatcaaa
    17161 agcaattgtt ccgcctgctt tcaaagaaaa aggttcgtct tttgatactt cttgaatcac
    17221 cgaatatttt cgtaaggcag ctgttgctgt tttatcgaac aaaatcagca tgggttcttc
    17281 cttgtttatt gcttctgtcc caatttctgt aatctttgcc tgaatcattg cactctctcc
    17341 tttaattgct ttcaaaaaca gtataacaca ttctcattct ttagccgtgt ggaataatta
    17401 tgtgatagaa taaaaacgaa aactatttca ttacaattag gaggaatcag ctcatatgat
    17461 cgaaaagata attttaggaa cctatacgcg tcgcgaaagt aaaggaatct atacgatcga
    17521 ccttgataca gaaaaagaag aattagccaa cctttcatta gctgttgaag aaaatagccc
    17581 tacctatgta gcaaaaagta aagctggcaa tctttacaca gttactagtg tggatggatt
    17641 aggtggtgcc ggtgcgtacg ataaggattt tcattttttg aatgcggtaa cagagtctgg
    17701 cgctcctttg tgttatgtag cggttgatga agcacgtcaa ttactatacg gggcgaacta
    17761 tcataaaggt gaagtaaatg tctaccatat attaggagat ggcgaactga aagcagcaga
    17821 ttcaatattc catcaagaag cgacaggtcc tcataaaaac caagatcatg cgcatgtcca
    17881 ctatacggat ctgactcctg accaacgatt agtcgtatgt gatcttggta cagatcgtgt
    17941 gtatacttat gatgtgtccg aaaacggcaa attgaatctt gtaactacct tcactgcaga
    18001 gccaggaaca ggccctcgcc atcttgtctt tcatccaaat ggacaattcg cttatttatt
    18061 tggtgaatta gacagcacag tccgcgtgct ttcttataat aaagaaacag gtgcttttga
    18121 agaattacag aaaatatcga ccttgccaga agaattcgac ggtgaaaatg gaggggcagc
    18181 tatccgcatt tcaaatgatg gcaagttcct ttatgcttct aaccgaggac ataactcgat
    18241 tgctgtattc gcagtttttg aagatggttc ccacattcaa aacaagcaaa tcatctcaac
    18301 agaaggggat tttccccgcg atttcgcact agatccaagc aataattttg tcgtgtgtgc
    18361 caatcagaac accgataatc tgacattata tcgccgtcat cctgaaacag gattactgga
    18421 attactgaaa aaagatgtct atgtaccaga atgtgtctgt gtttattttg atcgttagta
    18481 agaaaaggag aatgaaatga atcagcaaga cgtattagac agactacggg aagaactaaa
    18541 tattcctttc tttgatggga aaatcgaaga taaagagtat agcgaagagg aatatcagaa
    18601 actcaaagcc gatttacaga actactttga acaatatgta cagaatgtag aaaactaatc
    18661 agctttccat actatctgaa aagaacatca ggacaagact tttccgtcta tcctgatgtt
    18721 cttttctaca aataaaaaac cgccgagatg gttagactcg acggaaaagg agtatttact
    18781 cgattttgag taagtacaag gttgttgttg gttataggta tgtcattatc ataaaggatt
    18841 ttgatcactc tatcataaag gtaatattac atttatatta gataactcta gtttttattg
    18901 ttatcaaaga tttcttttat tttttcaaca acaaattagt cattttttgg atggcagctg
    18961 tcaattggtc tgtcgcttgg tcttgttttg cttcagatgt attgaataga tcttcatcta
    19021 cccctgattc aagatcgata ctttggatca tttcttcaca gcctgctaca tatgaaagat
    19081 aagctttttc aaaattttta tgaatgccca ttgcttttgc tggtggacgc aaagtactga
    19141 ctttcataag cattgcccga tatttttttg ttccttcttc aaataacagg atgatctctt
    19201 caagagtcga tttgtccagt tcacttgttt ttccttcatc aattgcgttt ctgatcactt
    19261 catatttcgg atgcattcct tctccgattg cttctgtttc cttgacgatt tgatcgatgg
    19321 actgcacata atagccaata ttcggtaaca tgttcgtttt cctactttct ccattgttgt
    19381 tattatttat tctacttgtt tttatcctca aatgacaagt acttgaaaaa atttaatggt
    19441 ttctatacag ctcgccattc ttattcgtgc tcaaagaaac catctgctaa actatattgt
    19501 ttccctgcga caatgtcata ttggatcaat tgatattcgt cgtaaatctc ttgttgcgag
    19561 cgattgaatt gcgcttcttt ataccactgt ccgttttgat agtacagttc tcgttcaaac
    19621 gccggaagtt cctgttctaa tgataaaagc agctgataaa agccatttgt tgtctgattc
    19681 gtctgttcca ttagattcgc tgcaaaataa aacggactgg tgattgcatc gtgagtcgtt
    19741 tgcttttcta attctccttt actgtcgaac ataagaaatt gtgtttcatg caatgtttgt
    19801 ttgtcatttt ttgcttggat cgtatctgaa tagatccctg gaagatgatc cccccaaaaa
    19861 acaactaaag tccttctaga aagattcttc aattcttccg tgaactgctt cagtgccgtg
    19921 ctgctatagg aaatatcttg cagataattc tctaagtcaa gtgtcccact tccatctgaa
    19981 gtttttgcag gatagtctag attatcatac tttccatcgt agggcatatg tgtctgcatc
    20041 gtgactaaat gaatgaactg aggtgttttt tcttccttga gaagtgtcat tacttctttg
    20101 taagctgact catcagaaat atatggatta ttctctatcg tatccgtata ggtcatcgta
    20161 cgctcgctga tgaactggtc aaatcctaat gtctgataaa catcctctct tttatacatc
    20221 gaagtattgt atggatggat cgctgtcgtc tggtatgatt gtgcgtttaa cgctgaaacg
    20281 atagaaggaa gctgatccat ttttggtaca agcatcgtgt aaggggtggt gagttgcgca
    20341 ttaaataacg ccatagaaaa accagtcaat gcttcaaatt cgatatttgc tgttccaccg
    20401 ccatagtttt gagaaagcat attgccacta tatgtttgat cagctacttc ataataatct
    20461 gccaatggct caccagatac ttctacgccg tttaatcgac taggatctga gaaactttca
    20521 ctcatcacga acacgatgtt cggcgactct tcctctacag ttttattttt ttcatcggct
    20581 agcttctggt atttttcagt gatttctttg atcttttctt tcgaatatcc ttcaggttca
    20641 tccattggtt ctaccttcag attgtacaaa aacccaccga taaaaccagt gttatagtag
    20701 ttcattttct ggctgtaagg tatccatagt gccgttttat tatatgcttt tcgcaacaaa
    20761 ttatcgggat tgttgaaatt actgatatac cctagcagtc caattgccac aagcagtgtc
    20821 gttaatcgga tcaattggaa tcgccttcct tttttcaggc ttcgaaacat gtaccagcaa
    20881 aacaatccta tcacggttcc tgcggcagct aagatcactg taaacagcat agtgccggtc
    20941 atatctttca gcaatccgat ttcagtgatc atcttcaaat cgtctggata aatcggttct
    21001 tgccgataac tcattttcat ataatttgca aaacctaata caccgatggc tacgacatac
    21061 gtaagggaac ctaaagggac agatcctgct agtgcaatca gaaaaataag aataatcagc
    21121 aagacgagac aggctaagaa gaatttttcc gtatgccaag aaaaagcaaa tttcaacgca
    21181 aggtctacgg atagatcatt ttgacaccac tgcaaaaata aattactaaa aacaacaaga
    21241 aaaaagatac tgatgattgc tgctgttcct tttaaaatct ttttctgttt ggtcgaaagt
    21301 ctgcttatat atcttttcaa atgcttgctc cttcatttct atcaataaaa gaatgttgct
    21361 tctcttattt tagttgtacg aaattcgaaa gtaaagaacg gaaagaagaa ttaatgaaat
    21421 ccaaatattc tctttcttta tggcctttct ggtttcttta ataagcggac ctttcgtact
    21481 ccagaaattt tttctagctg ttgtttcact tgattgagct gttcaaaatc agtactgtct
    21541 atgtcaatca atatgtagac atgctcattc ttatggttac gcgatatatc tgcgatcgct
    21601 agctcgttct cattcagcag agaaaatatc tctgctaata tattggtttt cttttgataa
    21661 aatatagtga atcgaaacgg cgcttggaaa aacattcgtg cggatggata attgaccgct
    21721 tctctaaccg taccgaacaa aagataatct tttaaggctt caactgccaa acgtccgctg
    21781 tctgccaaag cttcctgagt cgatccccct agatgaggca acataagtat acggtcattt
    21841 tctaagaact cttcttctgg aaaatcagtc aagtatttag ccagatgttg tttttccaat
    21901 gcttgcataa cagcatattt atcaacaatt tcacttcttc ctacattgat aagtacagca
    21961 tcttttttca ttttttctat attcgcctga tcgatcaatc cttttgtatc ttctgtcaac
    22021 ggcagcatga ctacgatgta atccgaggta gaaagaactg tttctaaatc tgcttgccga
    22081 acataatcca actgggcatc gcggatagaa taacccaaga catccatccc taagctatag
    22141 caacttagcg ctacttttgt gcctatcgct ccaagaccaa gaagaccgat ggttttgcct
    22201 tgcaattctc gtcccacata agcacttctt ttgttttctg cctgttctaa aatattagga
    22261 cctgttagtg tttgtaccat acgacttgct tcgattatcg gacgggaaga taagagcaaa
    22321 cagcacaaaa caagctcttt tactgcatta gcatttacac ctggtgtatt catgacgatc
    22381 gtcccgtttt cagaagcttt ttcaacgttg atcgtgttca ccccaacacc agctcttgaa
    22441 attgctagca aatctggtgt aagccattca tctttcactt tcgtactacg aataactatt
    22501 ccttcggctg gcaaatcatc tattattttg aaatattccc cttgtaactt ttctatctct
    22561 tgttctggaa tagcatcaat caaatgaacg tgatacataa aaccccctcc ttactaacaa
    22621 gtatctcgaa atttctgcaa ttagacaact gtttaccatt tgacttccta gaagcaaaaa
    22681 gagcagttag agcagagtag gctgagtgat aaaaataagg ctatgacaag ttactgccat
    22741 aagccttatt ttcgagagca aaatattgag ggtgagatat cttgataaat ggtgttccgt
    22801 tagctgacat tgataataag actaaatttc aaaaaagttg aaaagcaaat ttcgtcaatc
    22861 tcttcttatt tcttaaaact aagtggcttt ttcacaacct ctttttatct aataaccact
    22921 gtttccaact caactaattt tgcccacgtt tcaatttgat ctgttgttag atttagcgag
    22981 acaacggtat gatgaccgcc acctgcctga atccatgaat ggacaccttc atggaaattc
    23041 ggtttagttt tccataacac acgtgcaaca ggtaaattag gtgcttcagt cgttggttca
    23101 aaggcttcta cttcattgat cagcagttta taatgcgtac cgaaatctgc catcgatacg
    23161 actacccctt ctcctgcttt cccatcaaat acaagacgcg ccggatcttc tctatctccc
    23221 attgataaag gagaaacaac gattcttggt ttcgtcgcag caagcgtcgg atctacttcc
    23281 atcatatggg attccaagat tgcttcttgg ccactagcta gctcgtaagt ataatcttcc
    23341 atgaatcctg tatctttccc tcgagccatg attttcagca accgatcaat cgctgcagtt
    23401 ttccagtcgc cttccccagc aaaaccgtat ccttctgcca tcaaacgttg tacagctagt
    23461 ccaggtagtt gtttcattcc atacaggtct tcaaaattag tcgtaaaagc gttatagcct
    23521 ccttcttcta agaaacggcg aataccgatt tcctgctgag cttgtacttt tacatgttcc
    23581 tcccatgtct tttcttcata atcaccatag tcgaaggtat acaattcctt gtactcttca
    23641 aataattgat cgacttcttc gtctgatacg cgatcgatta cttgtacaag atctccgata
    23701 ccaaaataat caacagtcca accaaattga atctgtgctt ccactttgtc tccttctgtg
    23761 acacccacgt ttctcatgtt gtcaccaaaa cgagccactt ttattccaaa actttcatta
    23821 taggctactg caacatccat ccaatcagca atctcttttt ggatctcagg tcgtttccag
    23881 tagccaacga cgattttatt ttgtttattt aatcgagcat tgatgaaccc atattcgcgg
    23941 tcaccatgag cggattgatt taaattcatg aaatccatat cgatcgtttt ccaaggaatg
    24001 ctttcgttat attgcgtagc taaatgaagc aatggttttt gtagaagctt tgtcccgcgg
    24061 atccacattt ttgctggtga gaaagtatgc atccaagtga tcacacctgc tacttcttct
    24121 cgataattga cctctttcat gatttttgtg atcgtatcag cagtaactgc taattcttga
    24181 aggcggattg gataaggcaa ttggccattt tcattcagtt catcgaccat ttcttgtgca
    24241 tgtttcttta cttctcgcag tgcttcttcc ccatataaat gctgtgatcc tacaacaaac
    24301 caaaattctt tttctccaat attcaacata attagccact ccttttattt gttttgtccg
    24361 taatacgcat tttttccgtg ttttcgtaaa taatgtttat ctaaaatccg ttgaggcagc
    24421 tcttctgaaa atgaattgag ctgtctggta aataaattca tcttgcatac ttcatctagt
    24481 acgactgcgt tcattaccgc actttgcgca tctttccccc aagtaaatgg accgtgtccg
    24541 tgtaaaagta cccctggaat tgctaaggga tcaattttgc gttctttgaa tgtttctatg
    24601 ataacttttc ctgtttcata ttcgtacccg ctgtcgattt cctgttgtgt caaaaatcga
    24661 gcacaaggta ccgatccata aaaagtatct gcatgcgtcg tccccatcgc aggaacatcc
    24721 aatcctgctt gtgcccagat cgtcgcccat gtcgagtgag tatggacgat tcctccgatt
    24781 tgaggaaatg aacgatataa aacagcatga gtaggcgtat ccgaagaggg attgagttta
    24841 ccttcaatga tatgattgtc caagtccaca acgaccatgt cgtctgctgt catctcttca
    24901 taactgactc cgctaggttt gataacgaaa tatcgtgttt caggatcaaa agcgctcaca
    24961 tttccccatg tatatttgac gagtccgtgt ttaggcaagt caagatttgc ttggtatact
    25021 tcttctttca gttgttccag cattttctat cactcctttt tcttgcaatt gatgaatcgc
    25081 tgttttttca atgtcgatcc cctcacggta acgtctcata aatatctgat agcctgctat
    25141 ttcttctgct gaaggtgtga tggtgattcc ttggtcttgt ccaaacacgt cttctgccaa
    25201 gaagttttct aaagacttcc catgtcgatc catcatatag gctgctaata acgcgattcc
    25261 ccacgctcca ccttctccgg ctgtgtccat cactgtaact ggtgcctcca tagcagatgc
    25321 tagaatccgc tgtccaactt ctggtgtttt aaaaatcccc ccatgcccca ctaaacgatc
    25381 aatttggaca ttctcagatt tcaatatatc cattccgatt ctcattgcac caaaagcact
    25441 ggctaaatgg atccgcatga aattcgctaa atcaaaacgg ctacctggtg tacgtacaaa
    25501 taatggcctt ccttcattta ctcctgtaat gttttcaccg gaaaaataac cataagaaag
    25561 taatcccccg caatccgtat cgccttctag cgctttgttg aataataccg tgaataattt
    25621 atctctgctt atctccatac ccaaagaatt ggtaaattct tcaaaaatac tgacccaagc
    25681 attgatatcc gaagagcagt tattcgtatg aaccatgcct actagttctc ctgaaggtgt
    25741 agtgaccatg tcgatttccg gatggacatt ttctagttct ttttctaata cgatcatagc
    25801 gaaggcggaa gtaccagccg aaatgttccc tgtccctttg gctacactgt tcgtagcgac
    25861 cataccggtt cctgcatccc cttctggcgg acaaaccggg attcctggtc tcaaatttcc
    25921 cgtagggtcg atcagataag cccctgattc gcttaacttg ccagcttctt ctcctgctac
    25981 tagaacttta ggcaaaatgt gatccaagtc aatcgctacg tgcgcttttt cgattaactg
    26041 accaaaaata tttttcattt cttgatggta ggttttcgtt gaaggatcga taggaaacat
    26101 gcctgatgca tctcctacgc ctagtacttt ctcaccagta agctgccaat ggacataacc
    26161 tgcaagcgtt gtcaaaaaat taatttcttt cacatgttct tctttattca aaatagattg
    26221 atacaaatga gcgatactcc atctctgcgg aatattgtat tgaaattttt ccgttaatat
    26281 tcgttctgct tcttcagtga ttgtatttcg ccaagtacga aatggtacta gtaactgttc
    26341 cttgctatca aatgctagat aaccgtgcat cattgcactg aacccaatcg acccgattgt
    26401 cgtaagattc tcgccataga tttcgaatac ttctgctgcc atttttcggt aactgacgcg
    26461 cagtcctttc caaatatctt ctaatgaata ggtccagata ccattttcta gtttgttttc
    26521 ccactcataa ctaccagcgg caataggagt aaaagatgaa tcaatcaata cagctttgat
    26581 cctagttgat ccaaactcaa tccctaaaga tgtacgacca tctatgacat ctaacatact
    26641 ttgcgcattt acttccttca taatgattta actccctcct tctcgtcaaa cctgatcaaa
    26701 ccgtcttttt agtagcgctt acaatgatta ttgtatactt ttgtacgtac atgtcaagca
    26761 gttatttaca agaatacaat aaaaaacact tgttttgtac gtacataagt tataaaatga
    26821 aattgcttac aaaatctgat caaccgcctt ctcgaaattc tagaaggaga gaatacttat
    26881 gaaaaaagaa aaaaaagttt cctcatcgtt tatttatttt tttggcgcat ttggcggcat
    26941 tctatttgga tatgatattg gtgttatgac cggcgcctta ccttttttac agcatgattg
    27001 gggtttggca ggtaaagctt ctttgatagg atggatcacg tcttcggtca tgttaggtgc
    27061 gatccttggc ggctctttat ctggtcttct ttctgataag cttggacgac gaaaaatgat
    27121 tcttctttct gcacttattt ttatggctgg ttctgtcctt tctgcatctg cccctcacaa
    27181 tggttcttat tttctgattg cagcaaggat tcttctaggt ttagctgttg gcgctgcatc
    27241 tgccctagtt cctgcttata tgtctgaaat ggctcctgct agactaaggg gaagattgtc
    27301 agggatcaat caagtgatga ttgcttctgg catgctcctt tcctatgttg cagattattt
    27361 gctaaaaggg ttgcctgaaa cgatggcttg gcgcgtgatg ttagggctcg ctgctgtccc
    27421 cgcgctgatt ttatttttcg gtgttttagc tctaccagag tcaccgcgtt ttcttatgca
    27481 atctggccgc ttggaagagg caaaaagagt attgaactat attcggacac caaaagaagc
    27541 tgaacaggaa ttcgaacaaa tccagctgaa tgtcaaacaa gaaaagacaa ccggaacttc
    27601 ttggcatact ttgtttttag aaaaatatcg ttccttagtc attgccggta ttggtgtagc
    27661 agtattccag caatttcaag gagctaatgc tattttttac tatattccgc tgatcgtaga
    27721 aaaagcgact gggcatgcag cgagcgatgc attgatgtgg ccgatcatcc aaggaatcat
    27781 tttagttgct ggttctttgc tctttttggt tattgcagac aagttcaatc ggcgaacttt
    27841 attgaaaata ggcggtagcg tgatgggact ttctttcatc ctaccagctg ttttaggaac
    27901 tgtactggat gctcatacaa attcgttgtt gattctcttg tttttatgta tttatgtcgc
    27961 attttattct tgcacatggg ctccactgac atgggtaatc gtaggcgaaa tattcccact
    28021 agctgtccgc gggcgagcat ctggcctagc ctcttctttt aattggattg gttctttcct
    28081 agttggcttg ctttttcctg tgatgactgc ttctatgtct caagaaatcg ttttcggcat
    28141 ttttgggatc atctgctttt taggggtttt atttattcaa gagatcgtac cagaaactcg
    28201 tgggaaatct ttagaggaaa tcgaacaaag tgcatctaaa aaaacttatc ctaaacgtat
    28261 ttctatccat cattcttaaa gaggttgtga cagaagtggt cagcttcaag aaataagaag
    28321 gaaccgctta gtttccagtc acaaggaaca atttcaaaaa acagcttctc atgtttgatg
    28381 aatattgtga cttgtgacca gttggtagga gaacaccgtt tagtggagag gtggtgaaag
    28441 aaacgttcag ccttgagcga tgacaaaagc tctcacaaac attgcgacga gtaaccgtag
    28501 gagcaataag aggcggtaaa aacccgtctc ttatttttcg ttttctttca ctgtttcccg
    28561 ttcaactagt acaggttcat agagaatatt tggctgtgcc tgtccttttt caatcgttgt
    28621 gacgatccac cgtgcggcat cttgtcccaa cttttctttt ggatgcgtca atgttgtcaa
    28681 ttttttattc cctaaatggc ttaaagaaga atcgtctgtt cctacgatcg ataggtcatc
    28741 tggaatatga tagccatttt ctgtcaactt atctagcatt aaactagcta tttgatcgtt
    28801 atagcaaatg atgccatcta aagcttgttc ttctttcaac cgttgtactg ttttttcagc
    28861 gattgtttct cgtgtttccg tggtgaaagt aatgatatct tccgtcaaaa aacgcacatt
    28921 atactgttca cacgcgctga taaaaccttt catcctgtat tttccttgaa gatcgtcgat
    28981 ttttgtgatg aacaacagac gctgatgtcc tttttttatc aaatattctg ctgccataaa
    29041 accgactttc acatcatcta aacaaatata agaggcattt aactcttcat aagcagcatt
    29101 gatcatcacc atcggaatgc cttgttctct caaagcaaca taaagtgcta agtttgggtt
    29161 atactgatta cttttcgtag gctcaacgat caaaccatct accccataag caatcatttt
    29221 cttcaaacac tctttctcca attcgtgatc gttattcgta ctggctaaaa gcaatgaata
    29281 cccagattct cgtaattcat tttcgattcc tcggataatc gacggaaaaa tataatcaga
    29341 gagatatgtg gtgatgacac cgatcgtttt tgttctgttc aatggtgttt tttctttctt
    29401 atagctgtca tctacatatg tgcccgaccc tttttcttta cgcagatagc cctcatttac
    29461 caacacagcg attgcttgcc gaatcgtatg tctgctgacg tcatatattt cttgcagctt
    29521 gaattctgag ggaatggttg tatttacttc gtattcctca cgaaggatct tttgttttat
    29581 ctcttctgca atatcttgat attttgccat ttgccttgcc cctttttatt tgtacgtaca
    29641 tatatcataa cgagtctgca aggtttattc aagcaaaaat caaggtttct tgtgctcata
    29701 aggcatacgg aacaggttca ccaaattctg gaaatccttg ctcattccac gtaaagcatt
    29761 gcgcgttcgc gtgccgatta ggattgtcta acggatcccc tttttggttc ttctctggtc
    29821 gagcatgata gatcaaaata tcaaagcacc cgtcttctga aaccgtaaac gagttgtgac
    29881 caggaccaaa ttgcttattt tttttagaag aaacgaatac tggctccgat aatttatgcc
    29941 atgattcttt taataaaaga ttcttgtgct catcagccca cagcaggccc atggcatagt
    30001 tttcatctgt tgcactacca gaatacgtaa tgaatatttt cccattgcga gtaaggactg
    30061 ctggaccttc atttactgaa aaaccccgtt tttcccattc atactcaggt atagaaagga
    30121 ggatctgctt cccctttaac gtccaaggat tatccatttc ggatagataa agattagaat
    30181 tcccagtaat ttcaggatct tgttgggccc acacataata tagctttcct tgagaagaga
    30241 aagttgttcc gtctaaactg aaagtttccc ttttcgtttt tatttgtccc ttttctatcc
    30301 aatctgtatt cattggatct atatgctgat tttcaagtac gaacatgcgg tgatgatgag
    30361 attcatcacg tatagtttta tgattgcttg cagcaaagta aatataccat tttccattga
    30421 tataatggac ttcaggtgcc cagatcaatt cactcatcgg tccttcttca tgagcctgcc
    30481 atacaatcag gcttgcagca tgttctaatt cattcagtga ctttgccctt cttaattcaa
    30541 tcacttggta gcctggaacc gatcctgtaa aataataata accatctgta tgtttatata
    30601 cccaaggatc tgcccgttgc cgaacgatag gattattata aagtgtcttc atcataacaa
    30661 atttcctcac attttcttta ttctaattac attccaagag tatttttcta tccctacatt
    30721 taattcgtca tgtttttcta ttgaaaaata gtttatttca ggttttattt tttctggttc
    30781 ttcacgtgta tttttgtcca acagatcgct actggttaat gtgtagtgct ctccttgatc
    30841 tagcaaattg aaatcatgtt caatcgttat cttttgttgt tcatttgtgc gattgatcaa
    30901 gaaaatagtc agctgattgc cgttatctac gatcacactg tccacataag gaacattttt
    30961 tatcttttca caatcataaa cagggcaaat gatttgttgt ttcaatactt gacctttagc
    31021 atatttcgat aagtgttgca tcacataata agttggattc ctccatgctt gtcctttagc
    31081 atctgtcagt attaaaggta ttgtgtttac taacaatgat tgacaactaa tctttacgcg
    31141 atctgcgtgt cgtaaaatag ccagccctaa tgaaccaaat aataatgtat cttccatagt
    31201 aaagtaacac caatcgatgg gactaccgac ttcaaaatcc ctatgtcttt tttccggttg
    31261 ggcatgtaca ttccattcat caaacgctag atacattgtt ttctcacttc tcaaatatcc
    31321 ttttacataa tcacaagtag caacgatttc ttcaatttga cgatcgaatc gataagcttt
    31381 tgcaaggtat gtcgctgtat catcacgctc atttttagga ggtttagtca agtcttcttc
    31441 atcatactta tcaatatagt tatgcaaagc taaataatct acattctcat aagcttcttc
    31501 taatgcgata cgatcccatt cagggtacga tggtaaacgt ggcgtagagc ttccaactaa
    31561 gattgtttca attgaatcat ccactaactt catgactttt gaggcttcat ttgctaacct
    31621 accatattca taagcactct tagcccccac ttgccatgga ccgtctagct cattccctaa
    31681 acaccataat ttaacgccaa aaggcttttt ttgcccattt tttattctta aatcactcca
    31741 gaaggttcct tttggaaaat tacaatattc tactaaattt tttgcttcat caattccttt
    31801 tgttcctaaa ttcaccgtga atatcggttt cgcttgtaca tgactgatcc atttaaaaaa
    31861 ttcgtggagt ccaaaagaat ttggttctat acctcgccat gctaaatcaa ttttttttgg
    31921 tcgttgttct ttcctaccta cagtatccac ccaatcataa ccagatgtaa agtttcctcc
    31981 aggatagcga atgacaccta attctaattc ctttacataa gagaggacat cttgtctaaa
    32041 cccgtcacca tcagctgttt catggcccgg ttcataaatc cctgtataga cgacacttcc
    32101 catatgctca acaaatgagc caaacagccg agaactgatc tctcctaccg taaaatcctc
    32161 tctgattttt actttcactt cttcgtccaa cagctttccc tacctttcat ttcctatagt
    32221 caagataata gatacaagtt tccatacgcc accagcaaag ataaaaataa aaggagggaa
    32281 aagaaatatg agcagtacaa atcctaataa caatacgcaa caaagaatat ttctcaaaaa
    32341 atcaaaaaat actttccatg cactttgttc aaaaatttgt aaaaatgtga tatctttgtt
    32401 ttcagaaatc acatatatcc aggataaaag aaacatggaa taaataagta aaactacata
    32461 tccactaatt gaaagtacga ttgtctgttc ttctaataat tgattaacaa gaaaataaat
    32521 aatgatcgtt aacagactaa ggaacaagtt cttaccaaaa tgttgcttta ttccttgaaa
    32581 gaaaaaataa atgatatttc ctgatccctc acctttccat tgcgccatta cataaaaaac
    32641 agcacatgta gcaggcacga tagtaataac aggcaaacac acgattaccc agagaatacc
    32701 taataagatg taatcagaaa ccacaatcaa ctgtccataa agtttagtct caactatttt
    32761 catcttcctc actctttcat tccagacata gctgttcctt gaacaaaata tttctgtgca
    32821 aaaatataaa ccactagtaa tggcagcatt gaaataaccg ttccagccat tacaggtcca
    32881 taaaaagaaa tgttctgccc tgtaaataaa gctaaccctg ctggtaaagt tctcattttt
    32941 gaactacttg tcaatatcaa tggataaagc aagtcattcc aactattcat cagcgtgaaa
    33001 atccctaatg ataaaagagt aggtttgctc aatggcaaca tgattttcaa aaaaatatta
    33061 aactcactta atccatcaat gcgtgctgcc tcttccaaag aatctggtaa agtaataaaa
    33121 aaggaacgca tcatgaagat accaaatgca gttgtcatac gaggaatgat caaacctgca
    33181 taagtatcaa gcatgccaag caaattgact tcaataaata gaggaatcat gaagacttgg
    33241 aatgggatca tcattgtcag caaaacgaaa tagaacaaaa ctgattttcc tttgaaacgc
    33301 atacgagcaa atgcataccc cgcaagagaa tcaaagaata cagaagtgat cactgtccct
    33361 ccagcaaaaa tcactgtatt tttgatgtaa tctaataaag ggatcgttgt ccaaacctta
    33421 atatagttat ctagggtata gctatctgca aatattctag gcggaaatga gataatgtct
    33481 ttttcgtgtt taaaagaaga aataatcagc cacaaaaaag gaaacgcaat aaccaacaat
    33541 ataactagca tactaatcgt tcgaatgatt tttttaccac taactgcttt tcgactattc
    33601 acagtaactg taatcgagct ttctggtgcc atacaggttt ccctcctatt tcatttgttt
    33661 ttctttccgt gacatgtaca cattagtgga gatagctata gttgcaacaa tgataaacaa
    33721 aaccgaagac agtgctgatg catagcccaa ttcataaggt gctgtaaatc ctcgactgta
    33781 aatatattga acagctgttt ctgtcttaaa ttgtgggcca ccagcggttg caacatatac
    33841 ttggtcaaac acttgcatcg aaccaatcag attcgttaaa atacaaaaac ctaaggaggg
    33901 aacgatctga ggtagcgtaa tgttaaaaaa ctgctgtcga tttgttgcac catccatttg
    33961 agcagattca tacaaagagg gagagattcc ctgaatagca gtcaagagaa tcgtcatagt
    34021 aacaccaaaa ttcttccaaa cagtcatgat ggctaccgtc ggcatagcaa gggttggatc
    34081 gcgaaaaaat tgagggatcg gcaagccgac ttgtgaaaac aagtaaggaa tcgcccctac
    34141 attcggatca agcaacatag aaaaaataat cgcaattgac gtcaatgaac aaataactgg
    34201 aatgtaaaat gtcgaacgac aaaaacgatt gaatctggta tttttagata atgctactgc
    34261 tgctaataaa ccaacgataa tttgtgttgg cgtttcgagt acggtgaaat ataaagaatg
    34321 aatcaacgaa ttaactgctc gttcatcact aaatatacgt gtgaaatttt caaaacttac
    34381 aaaatttctt gatgtaaaga aaatattcaa atctgtaaaa ctaatagcaa atgttcctaa
    34441 aagcggtatg acagtaaata ataataaaac taaaaaagat ggagttaaaa acaaatacgc
    34501 agcacgatca ggacgattcc aatatttggt gaatccgttc atatagtcca cctcatcttt
    34561 cattttgata aaatatacaa tcttggttta aacgaatctg aataagatat ccgaacaatc
    34621 gctgtgattt ttgcaataac acctatctgc aaatactagg tcgatcattc agatatctgt
    34681 atcttgccaa cagtctatcc tgtataaagt tctgtactat ttttgcagta attgattgat
    34741 ggcatcatct gcatcgttca tgagtgtctc aggatcttct cctgctaata atttttcaat
    34801 cagtggattg atgatatcgt tattgatcgc agagatttgg gtaaaacctg gtaaatatgg
    34861 ctctgcatag tccatttgtt tcgataattc agatacaata ggatcatttt ttacttcttc
    34921 atcttttgct actgaatcca agtaggcagg aaaaccattt tcgatactcc attttttacc
    34981 aatttcagtt gtattccaat atttcaaaaa ctcatagaca gcttcttttt tattattatc
    35041 tgtacttgcc ggaatggcat atcctacgcc atctaaaata gctgtttttt tgtctgaatc
    35101 actcccttgt ggtaaggtag tcacattata atcaatctcg tttgctttca atccgttatt
    35161 taaccatggc ccattgattt caatggctag ttgaccagca ttcattaaat tgtccatttc
    35221 ggcgccagaa atcgattctg gaccagtctt gtctacatgg atcatttttt ggatcgcttt
    35281 taacgtttta aggttctcag gagacgcaaa ttcagactta gtataatcat cgttcactaa
    35341 tttaccgcca tttgctaaca tccagttata taacggtgca gttccatcct tattgaagac
    35401 aaaacctgat acatttttac tactatcagt taattttggt gccatttctg tcaattcttc
    35461 ccaagtagta ggtggttgtt cagtatctaa tccagcttgt ttaaacaaat cattattcca
    35521 atacatatac atgccttgta cctgcatggg gacaaaatac tgtttacctt ctacttcacc
    35581 taattcaata gccgtttctt taaaatcaga tttatctact ccatcatatt cccagaaatc
    35641 gtctaatggt tgtaccgctc cgttttctac atactgtgca aaatctggat aatttaatgc
    35701 aataaagtca ggtgcttttt tagaagaaat cgctgtagga agtttttcat tcaaattggc
    35761 ccaagtcatg atatccattt caattttgat gtttttatca tttgttttat taaattcatt
    35821 aacgatttct tgcaatattt caccatcaga tgcagtaaac ccattccaaa aagtcaaagt
    35881 cacttcttta tcagaatcag attctgctga acttccgcca ccgcaagctc ctaaaatacc
    35941 tgttgcagct accaacaaag agccaattaa ccatttttta cctttcattt tttgttcctc
    36001 cttcatctga taaacgacat ctgaccacat tccaagatag cggtttcaat aaacaattga
    36061 ttttcccatc ttcagctttt acatttgttg aatgacttgg ttttacttgt tcacttttag
    36121 gaagattagt tgcttttgta tcaaatccac tcatttcaga aaaatcaatc actccctcta
    36181 atgataattc agacaagtga acagttagat tcatttcttc ttctccacgg ttaactgcaa
    36241 atatgacaag ttctttgtca tttattacgg ctatactttc taaatacggc acttcttcaa
    36301 aatcttccgt cgcatagttt gctgaacgaa tttgtgggct taacacctct ccttgtccat
    36361 acacagcagc ttgcataaat ggataataga tcgtctgtct ccaagcttct ccgtttgttt
    36421 ctgtcctgat tggtgcaata acatttacca attgagcaag acaagcaatc tttactctat
    36481 ctgcatgttt caataaagtg attaatagac agcctaatag taatgcatct tcaaaattat
    36541 agttatcttc tagtaaagga ggagcaatct gccaaggcgc tgcttgctca tcttgttccg
    36601 ctgaatgata ccaaatattc cattcatcaa aggacaggtt gatctgcttc ttactatgtt
    36661 tcttcgcctt gactgtatcg cacatcgctg tcacttcaga aatgaaccga tccatgtcta
    36721 aggaacgtgc aagataatga gaaagatcat ttttttggtt gccataatat tgatgcagag
    36781 acaaataatc tatttgatcg tacgcttgat caagaactgt taactcccaa tcaccaaatg
    36841 taggcatttg ccgattcgaa cttccgcata aaaccacctc aattgaatca tctacgagtt
    36901 tcatcgcctt agccgtttcc tctgctaacc gtccatattc ttccgctgtt ttatgtccaa
    36961 tctgccaagg accgtccatt tcattcccta aacaccatgt cttaattcca aaagggctct
    37021 cttgaccatt cgaacgcctt aaatcactcc aataagttcc tttaggaaaa ttacaatatt
    37081 cgactaaatt acgagctgca tctactccac gagtacccag attgacagcc atattgattt
    37141 cggcccccac atgctgacac cagttataaa attcatgaat cccaacttca ttcgtttcaa
    37201 ttgtccgcca agctaaatcc aaccttctag gtctatttgc tatgggccca atactgtctt
    37261 cccaacgaaa tcctgacaca aaatttccac caggatatct aatcatggga atgttcaatt
    37321 cttttaccaa gtgaatgacg tcttggcgaa atccactttt attagctgat ggatgatctg
    37381 gctgataaat cccttcatat actgctcgtc ccatatgttc aataaatgaa ctgtacaacc
    37441 gcggatcaac tttgcctaca cgcttgacac tactaatcaa cattgtggct tccataactg
    37501 acctccttta atttatacat gaataataca cctcgttgaa agcgttgtca ataatataaa
    37561 tgttaaactt acttattttt tatcgatttt aattaaccaa aatattaata atgattaaaa
    37621 caaataattt aatataactt
//
LOCUS       DACCYH010000017        37386 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.017, whole genome shotgun sequence.
ACCESSION   DACCYH010000017 DACCYH010000000
VERSION     DACCYH010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.017"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..616
                     /locus_tag="IVY85_000847"
     CDS             <1..616
                     /locus_tag="IVY85_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8043881.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            613..1386
                     /locus_tag="IVY85_000848"
     CDS             613..1386
                     /locus_tag="IVY85_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8043882.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1383..1781
                     /locus_tag="IVY85_000849"
     CDS             1383..1781
                     /locus_tag="IVY85_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP8043883.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1966..2637
                     /locus_tag="IVY85_000850"
     CDS             1966..2637
                     /locus_tag="IVY85_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043884.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2696..3136
                     /locus_tag="IVY85_000851"
     CDS             2696..3136
                     /locus_tag="IVY85_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043885.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3148..3816
                     /locus_tag="IVY85_000852"
     CDS             3148..3816
                     /locus_tag="IVY85_000852"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8043886.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3880..4503
                     /locus_tag="IVY85_000853"
     CDS             3880..4503
                     /locus_tag="IVY85_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP8043887.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4559..4945
                     /locus_tag="IVY85_000854"
     CDS             4559..4945
                     /locus_tag="IVY85_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP8043888.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5158..6549
                     /locus_tag="IVY85_000855"
     CDS             5158..6549
                     /locus_tag="IVY85_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP8043889.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            7160..7528
                     /locus_tag="IVY85_000856"
     CDS             7160..7528
                     /locus_tag="IVY85_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8043890.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            7708..8649
                     /locus_tag="IVY85_000857"
     CDS             7708..8649
                     /locus_tag="IVY85_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP8043891.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            8718..9449
                     /locus_tag="IVY85_000858"
     CDS             8718..9449
                     /locus_tag="IVY85_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP8043892.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            9593..11773
                     /locus_tag="IVY85_000859"
     CDS             9593..11773
                     /locus_tag="IVY85_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP8043893.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            11793..12275
                     /locus_tag="IVY85_000860"
     CDS             11793..12275
                     /locus_tag="IVY85_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP8043894.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            12291..13052
                     /locus_tag="IVY85_000861"
     CDS             12291..13052
                     /locus_tag="IVY85_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP8043895.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            13183..14517
                     /locus_tag="IVY85_000862"
     CDS             13183..14517
                     /locus_tag="IVY85_000862"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP8043896.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            14824..15309
                     /locus_tag="IVY85_000863"
     CDS             14824..15309
                     /locus_tag="IVY85_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP8043897.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            15302..15985
                     /locus_tag="IVY85_000864"
     CDS             15302..15985
                     /locus_tag="IVY85_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP8043898.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            16111..18756
                     /gene="polA"
                     /locus_tag="IVY85_000865"
     CDS             16111..18756
                     /gene="polA"
                     /locus_tag="IVY85_000865"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP8043899.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            18801..19637
                     /gene="mutM"
                     /locus_tag="IVY85_000866"
     CDS             18801..19637
                     /gene="mutM"
                     /locus_tag="IVY85_000866"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP8043900.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            19601..20230
                     /locus_tag="IVY85_000867"
     CDS             19601..20230
                     /locus_tag="IVY85_000867"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP8043901.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            20282..20614
                     /locus_tag="IVY85_000868"
     CDS             20282..20614
                     /locus_tag="IVY85_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8043902.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            20926..21417
                     /gene="nrdR"
                     /locus_tag="IVY85_000869"
     CDS             20926..21417
                     /gene="nrdR"
                     /locus_tag="IVY85_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP8043903.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            21440..22813
                     /locus_tag="IVY85_000870"
     CDS             21440..22813
                     /locus_tag="IVY85_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP8043904.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            22813..23739
                     /gene="dnaI"
                     /locus_tag="IVY85_000871"
     CDS             22813..23739
                     /gene="dnaI"
                     /locus_tag="IVY85_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP8043905.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            23868..24308
                     /locus_tag="IVY85_000872"
     CDS             23868..24308
                     /locus_tag="IVY85_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP8043906.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            24452..25153
                     /locus_tag="IVY85_000873"
     CDS             24452..25153
                     /locus_tag="IVY85_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP8043907.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            25150..26271
                     /locus_tag="IVY85_000874"
     CDS             25150..26271
                     /locus_tag="IVY85_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP8043908.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            26286..27518
                     /gene="pepT"
                     /locus_tag="IVY85_000875"
     CDS             26286..27518
                     /gene="pepT"
                     /locus_tag="IVY85_000875"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP8043909.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            27650..28558
                     /locus_tag="IVY85_000876"
     CDS             27650..28558
                     /locus_tag="IVY85_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP8043910.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            28593..28865
                     /locus_tag="IVY85_000877"
     CDS             28593..28865
                     /locus_tag="IVY85_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP8043911.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            28876..29922
                     /locus_tag="IVY85_000878"
     CDS             28876..29922
                     /locus_tag="IVY85_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP8043912.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            30173..32782
                     /gene="clpB"
                     /locus_tag="IVY85_000879"
     CDS             30173..32782
                     /gene="clpB"
                     /locus_tag="IVY85_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP8043913.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(32933..33604)
                     /locus_tag="IVY85_000880"
     CDS             complement(32933..33604)
                     /locus_tag="IVY85_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043914.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(33597..34091)
                     /locus_tag="IVY85_000881"
     CDS             complement(33597..34091)
                     /locus_tag="IVY85_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP8043915.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(34111..35331)
                     /locus_tag="IVY85_000882"
     CDS             complement(34111..35331)
                     /locus_tag="IVY85_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8043916.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            35478..37313
                     /gene="lepA"
                     /locus_tag="IVY85_000883"
     CDS             35478..37313
                     /gene="lepA"
                     /locus_tag="IVY85_000883"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP8043917.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT        12417 a         6550 c         8101 g        10318 t
ORIGIN      
        1 gtttgttttt gcaccgttag aaggagaaga cctcgcttca tttctggttg gtgcggcaat
       61 catgatgatc ggaatgacgc tctttttgtt tggagcagat tattctatga tggaagtcgg
      121 agatctagta gggaaatata tgatcaaaaa gaaaagtttg accgttttgg tcagtttggg
      181 atttgctatc gggatcgtga tcacgattgc tgaaccttcc gtgcaagttt tgggacaaca
      241 agtatatgat atatctgacg gagagattgg cagagtgttg ctgattggga tcgtcagcgt
      301 cggcactgga gtatttctag cctttgcgct gcttcgggtc gtattcaagc tatcctatta
      361 tcaattgatg gctataggct acataggcgt tttgatcgcc tcatttttca ccagttccga
      421 atttatgccg attgcttttg attcgggggg agtgactact ggtccagtaa ctgttccatt
      481 tatcttggcc cttgccggag ggatgactag tatgatcaaa caaaagaaaa atgaaaatga
      541 cagttttgga atggtcggta ttgcttcgtt agggccgatt cttgccgtga tgattttggg
      601 ggtgatcttc caatgagttt ctggcagcaa gtgatagatg gaatcgaaat cgttaccatg
      661 gaagtcattt tggcagtggc acctatcgta atcctatttg ttttaatgaa tatcattagt
      721 tttcgtatgc gcaaaaagcg tttctttgac attatgaaag gtttcttgat aacgactatt
      781 gggttgatcc ttttcctgca cggagtcaat atggcttatg taccggtagg acagcattta
      841 ggcagtgcta ttgcgggatt gccttacaat tatatactgg ttccccttgg ctttttaatg
      901 gggttcttag tagggtttgc tgagccggcg attcatgtaa tgatcaaaca ggttgaagaa
      961 ttgagcgagg ggagaatccg atcgaaaatc atgctgatgg taatttcaat tgggatcggc
     1021 gcagcagtag gattgtctat gtggcgtttg ctcgtcggat tctctcttta ttatttcttg
     1081 atcccaggat acgggctagc atttatttta ggacgaaaag tggacaagat ctttttagca
     1141 atggcttttg ataacggtgg agtagcaacg ggcccgatgt gttcgacatt tattttatca
     1201 atgagcgtgg caatcgctgc acaaattgaa ggaagagatc cgatcattga cgggtttggt
     1261 gtggttgcaa tgattgcatt gacaccaata ttatccactt tagtactagg atatatttat
     1321 aaacgaaaag atgaaaaaga cagaatcgaa aaagaaagat tgcaaaaatt gggggaaagc
     1381 aaatgaaaga tcttgtagca ttgaatcttc acatgattgt gacgatcgtt gacggaggaa
     1441 tgggcgaaga tgttatcgac ttttcaaaag aagcaggtgc agcaggcgga acgatcttac
     1501 atggaagagg ttctggtgta catgattcag ggaaattttt aggtttggac atttcgccag
     1561 aaaaagatat tgtgttgaca ttagtacccg atagtttgac gaataaagta atggatgcga
     1621 ttggtcaagg catcaacatc gatattccag gtaatggtat ttgttttagt attgatgtag
     1681 acaaagtcat tggtatcact aagattcatc aatatcgaga aattatcgga atgcaagaac
     1741 ttttagatga aaataacgat agagaaaagt ctgaatcata gacaagcgtt tgaagaggaa
     1801 ataaacacaa acaagaaggc ggatgagcgt tatttccgct tttttgtttg tatttttctc
     1861 gtgaatactt atgaaatagt aaatttttat gaaattctac tctaagcatc agactttctt
     1921 ttcaaaatat gctattttaa aaatgatgaa aaaaggggga gaaaaatgaa ttttttagtt
     1981 gttttaaaag atactctgat tgagcgattt acatttctat atgttcaatt gcaggaaaat
     2041 aaaacattgc tgcatttttc accagagttc catctagagg ggctaacatt aggagaaatc
     2101 tatcaagcag aagggctgtc ggctgttttg cactttttta aggtagaatt ggaattacca
     2161 gtaaaagatt atatcgagct ttctcttgaa tcttgggatc gagcagtggt tgaactgttt
     2221 ccagaaggtt taatgatcga tacaaacgat gggaagatcc atctgactgc agcagaactg
     2281 caaaaacaaa tgcgttatca aatagatgac gaaaattctt tttctatttt tagacgtcag
     2341 caaaagattc taaaaagctt tttaaaagtg atcagtaaaa aacggaatct gatcaaaacg
     2401 acacagctgt tgaaaaaata tccaggtctg cttcacacgt ctattcaatt tcctcaattg
     2461 attacgatga tccatcgttt tatacgtatg caacagctac catctaagaa gctgtttctt
     2521 ccattgacgg acacatttcg agtagtcaac agagaagata aaaagaaagt gattgtgatt
     2581 gactttacca gaaatcggaa tgtccttcac caagttgcaa tggaaaaggc caattaaggc
     2641 tttttctttt tggatttttt tgtcataata aatattagat gatggaggag ataatatgat
     2701 gcaaacatgg ttagaaaggc taatacgatt tcagcagaac tatcacaaac ttatgcaggg
     2761 aagatatgcg aaatttgatc agttgaatcg cggtctattg atcgctgcgc ttgtctttag
     2821 tttgctaagt cgttggttgc cctatagaat cggacaattg ttgttcatcg tctttttcgg
     2881 atggatgata tttcgtttcc tatcaaaaaa aatctacccc agactaaatg agaatcaacg
     2941 ttatttgaaa tatcttgata gagtcaaaga aaaatatcat cagataaaaa acaaaaagca
     3001 agtcaaaatg gatcgaaaaa gttatacttt ttttgagtgc ccgaattgtc atcaaaagca
     3061 gcgtgcacct aaaggaaaag gacgaatacg tgtgacatgc aaaacatgtg gtataaaatt
     3121 cgaaacgaat gtttaaggga gaaaagaatg aaattagtat tattacgtca tggcgaaagc
     3181 gaagcaaatt ttgaaaatta ctggacagga tggctagatg tcgccttaac agaaaaagga
     3241 aaagaacaag cagcagttgc cggtcaaaaa atgcttgaag ccggacttca attcgatgcc
     3301 gtttatacct ctgttttgaa acgtgcgatt cttaccggac aagtcgcact agaagaaatg
     3361 ggtcagttat ggcttcctat aataaaaaca tggcgtctaa atgaacggca ttatggggat
     3421 cttgtgggta aaaacaaaga taagatgaaa gaaatatacg gaaaagatca agtaaaaaca
     3481 tggcgcagag gatatacgga agttccgccg ttaaccgcag aaaatagatt tgaccgcaga
     3541 tatgatcact tggatccgca tttgatccca tacggagaaa gtctggaaat gacagtgaat
     3601 cggatcattc ccttatggca agatcatcta gcaccaaaac taaaagatcg gcaagattta
     3661 ctagttgtgg gtcatggtaa tagcatccga gcactgacaa aatatttaga agatgttcct
     3721 gaagatcaaa tggatacgat cgatatccca aatgcacaac caatccagta tacattagca
     3781 gatgatttga caattctgga caaaaaaata ctttaaagaa cacctttatc taaaagtgag
     3841 agatagaaaa aagaagcaaa ggaatggaac aaaaggaaca tgaaaaatta tatatgggac
     3901 tttgatggta cattgtttga tacgtatccc gcaatggtag atggtgcatg gcaagcacta
     3961 aaagattttg gtatcagtat ggacaaaaaa gaaatctact tcaaaatgaa aaagtattct
     4021 actagttatt tgatcaatga atcaaacttg aatgccagag agtttaatga attatttcac
     4081 cgatatgaaa aagaaagtac ggaagtttcc cgtccatttc cagaaacgaa acaagtactg
     4141 gaaatgctaa aagacaacgg cggtcgtcat ttcatcctga cccaccgttt gacagaatca
     4201 acatggggcc tgttaaaaga acatcgattg gcacatctga ttgaggaagt agtagggatc
     4261 gatcaggact ttcctcgtaa acctgatcca gcatctttaa attatttgat cgatactttt
     4321 catttagaaa gaacagacac gatgatgatc ggcgatcgtc gtttagatat cgaagcaggg
     4381 aaaaatgcag gagtcgctac ttgtctttac gacatcgatc attttctggg tgagatcccg
     4441 gcagattatg tggtaggaaa tctgaatgaa atccttacct tgcagagcat cattaaaaag
     4501 tagtatcccc tttcaaagga gatatgttat aattttcata aaggagtgat aaaattatat
     4561 ggaaatcaga agacaaaaag aagttgtaga agcatttcat tatgatatga gattgccgaa
     4621 ccaagaagta gaaacagatt tgaaagtagg cttctcgcct ttaaaagaaa cgatcgaaaa
     4681 ctatccgaaa gaacatagca tcgtaggagc acgcttagag ttccgtttag tgtttgatac
     4741 atatgtttta tctggaagtg tgagtcagat caatcatatc ctcaatcgga aaattgaagg
     4801 tcaaaatgat gtcacacaag aagaagtgga cgagctagta gcaccgttat tcgatatcgt
     4861 acgccgaatg tcttatgagg tgacagagat cgccttagat cagcctgggg taaaattgaa
     4921 ttttcaatca gaaaaacaag aatagacagt aaaaacatac ctgcgataga ttaaagctat
     4981 cacaggtatg tttttttatt actaattgta agcaaaaaga atgtcatttc gataaaaatc
     5041 gtctaaaata taaaagtcac tgaaaaaaat tttttcagga ggatggttga agaatcattc
     5101 tcgggaacct ttacttcctt cgatggactt gtcattaata atatgaggag gagaaaaatg
     5161 acaacactca tcacagtttt agtggttcta gtttttgcgg cattattata tgtgttctat
     5221 cgtatgcaaa agcgccatac aaaattttca acacgcgtct ttacagcatt aggagttggt
     5281 atcgtctttg gcggtctgct tcaattgatc tttggtgtag acgacaaagt cgttactcaa
     5341 tcaatggaat ggatcgggat tgtaggtcaa ggatatgtat cattcttgca aatgctagtt
     5401 atgcctttag tgtttatttc gatcattggt gcattcacaa aaatgaaagt ttctgaaaag
     5461 ctagggaaaa ttagtgcgac tgtattgact tctttacttg gaacaacagc tattgcagca
     5521 ttgatcggaa ttggttcaac aatgctgttc ggactccaag gagctaaatt cacacaagga
     5581 gctgcagaaa cagcacgaat ctctgaactg gcaacacgtc aggaaacagt tgaaaactta
     5641 tcgattccac agcagatttt atcctttatc cctaaaaacg tttttgctga tttagcaggt
     5701 agtcgtgcaa caagtacgat tggagtcgtt attttcgcgg catttgtcgg aatcgcttat
     5761 ctaggcgtga aaagaaaagc gccaaaagaa gcagcattct tttcagattt agtcgacagt
     5821 ctgtataaaa tcattatgcg gatcgtgacg cttgttttgc gtttgacacc ttatggagta
     5881 tttgctttga tgacaaacgt tttggcaaca agtgattttg cagctatcgt caatttaggt
     5941 aaatttgtcg ttgcttctta cgtagcattg cttgctgttt tgattgtcca ctcactgatt
     6001 ttgattggtg caaaagtcaa tccaatcaac tatttcaaaa aagcctttcc ggtattgagc
     6061 tttgcgttta cttcacgttc aagtgctgga gctttgccat tgaatatcga gactcagaca
     6121 aaagctttag gagtagatga tgcaacagct aatttctcag caagttttgg tttgtctatt
     6181 ggacaaaacg gctgtgctgg cgtttatcca gctatgttgg caacgatcgt cgcaccagca
     6241 gctggagtga atgtctttag tttggaattt attttgatgc ttgtggcaat tgtaacaatc
     6301 agttcttttg gtgtagctgg agtcggtggg ggcgcaacat ttgcttcact gatcgtttta
     6361 ggggcaatga acttgccagt agcaatcgtt gggttagtta tctctgttga accattaatc
     6421 gatatggcac gtacagcagt aaacgtcagc ggtagcatgg tagctggtat cgtgacaagt
     6481 gcacgcatca aagatttaga tcgcgatgtc atcaatgatg attctaaagt gatcgatgtg
     6541 aatgcatagt caaaaagagg ttgtgataga agtggtcagc tttaagaaat aagaagaagt
     6601 ttcctaaaaa tagtttctca aatttttgtg aaatttcggc ttatttccga gaagctactt
     6661 ctgaaacacc gtgaataaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag
     6721 ccaaacaaaa atagttcctc atattttacg tttggttgag cttattaccg cagatgcaag
     6781 cttttgaaca ccgtttaaga aagaggtcgt gagcctgccg tttcactctg agtcacaaag
     6841 agcaatttca aaaaatagct tctcatgttt gttgaatatt gtgtcttgtg accgaggagt
     6901 tgtcaggaga acaccgttta ccagaggttg tgacttgttt ttagtcacga cctctttttt
     6961 tatttattcc atataaatac acattattta ttccgaaaag tgaaagcgca aataacaaaa
     7021 acaaccaact tatttattat taaagaaaag cgtatttaag taggacgatt ctgttgagag
     7081 ctttcgtaga ttttcccatt ttcctatggt aaactgacaa agttgagaat gttagaacag
     7141 atcgtttagg aggaaacaga tgtttacgaa tgaaataaaa atcatgcttt atgtggacga
     7201 tgtagaaaaa aatgcaacgt tttggagagc agttggcttt gcggaaaaag atcgtcaaga
     7261 aatggatggg acgcttataa ttgaagtagg agttgaggac agtcaaacct catttctgtt
     7321 atatgatcga gcatttgttg agtcccattc acctggcaca gctactagtg ccccatcttt
     7381 gatgttttac agtaaagata tctttggact atataaaaaa atgcagacgc aatcagtcac
     7441 attaggtgat atggtacagc ttggagaaga atatgtcttt aactttgctg atcccgatgg
     7501 caactacttt gcggttacag gtaaataata gaaataaaga ctttgttttt ctgtgcgaaa
     7561 caggaaagcg aagtcttttt aattcaaata ataatattat gtaaacaaaa aactttccat
     7621 gcgtaattat acacaagcaa ataaatcgtc aaatgcgaac gccttcttta tactgtttag
     7681 gaacgaaaag aaggaaggaa gaaatacatg gattatcaag tactgcttta ttacaattat
     7741 acaaaaatcg aagaaccaga ggaatttgcc aaaaatcatt tagccttttg caaatctttg
     7801 gatcttaaag gtcgtattct agtagctgaa gaaggaataa atggtactgt gtctggaacc
     7861 gttgaaaaca cgactgccta tatgaaagca atgaaagctg atcctcgatt tgctgatacg
     7921 atgttcaaga tcgatccaag cgaaggacat gcctttaaga agatgtttgt aagataccgt
     7981 ccagagcttg taagtttaaa gctagaagag gacattgatc caaaccaagt gacaggtgcc
     8041 tatctttcac cgcaagaatt taaagaagct attttagatg aacagacagt ggtgattgat
     8101 gcacggaatg attatgaata tgacttggga cattttcgag gggctgtacg cccagatata
     8161 cgcagttttc gagagcttcc agactggatc cgcgagcaca aagaagaatt tatggaaaaa
     8221 cgggtagtga cctactgtac tggtggcatt cgttgcgaga agttttctgg ttggcttgtc
     8281 cgtgaaggat tcaaagatgt cggacagctg catggcggta ttgctactta tggtaaggat
     8341 cctgaagtcc aaggagaatt atgggacggc aagatgtatg tgtttgatga acggatcgct
     8401 gtcgatatca accaagtaga taaacaaatc gtcggtcgcg actggtttga cggaacacca
     8461 tgtgaacgat atatcaattg tagcaatcct gagtgcaatc gtcagattct tgcttccatt
     8521 gaaaatgaag aaaaacattt aggagcatgt agcatagagt gtgcgaaaca tccgcgcaat
     8581 cgctatgttc aagaccatgg aatctctcca gaagaagcag aactgcggtt tacagcttta
     8641 attaaataga aaacaatagt tttgattgtc gaccatttcc aatatgcgct atactagcgt
     8701 tgaggatggt gatccacatg aaatcattga tttatgctat tggggatgtc catggcaaac
     8761 atgagctttt tgaaaaactt attcactatt atgatccaaa gatccatcag cttgttttat
     8821 taggggatct caatgatcga ggtcctcgta caaaagaatg ttttttatta ggaaagcaat
     8881 tagtcgaaga aacaggagcc atttacctcc ggggaaacca tgaagaatat tttttgcaat
     8941 ttctgcaatc accggaggac tggtatgcgc cttatatgag aaatggtgga aaagaaacgt
     9001 ttgaaagcct gattcaccca ggggcatcgg aggaatactc cccaactgaa atctcgatga
     9061 tgatccaatc gagatattca gaactgatta aatttttagt ccagcgcccc ttgtattatg
     9121 aatgggaaaa ttatcttttt gtccatgccg gtgtggattt gacaaagaaa gactggcgta
     9181 agacaagtct aagagacttt atttggataa gggaaccatt tcatgaaggg aaaaacaata
     9241 caggtaagac aattgtattt ggacatacga ttacaccaat gctccatgga gatatgcaga
     9301 caacagacct ctggattcag gatcataaaa tcggaatgga cggtggggct gtattcggag
     9361 ggtcattgca tggggttgtt tttgacaagg aaggtattct tcaagatatt gaatatcaga
     9421 acaacgaagg accttggcat ggaatctgac taatggaaag aatgaaggag ctgtgacttt
     9481 gttgcggctc tttttatttt tatagagaat attgtttctt tcagtagttt aacccaggat
     9541 aaaaagtatg atagaataaa gaagcaattt ttagcagaaa agaggcattt taatgacgga
     9601 aaatttgaac cagacgatca ttcagcttgt acaaaaagaa ctttcagact atcgtcctaa
     9661 gcagttgact actgtactta atttgttaaa tgaaggaaac actgttcctt ttatcgcgcg
     9721 ttaccgaaaa gaaatgactg gaagtcttga tgaagtccag attcgtgaaa tcgaagagcg
     9781 gtatgcctat cttgaaaact tagaaaaacg taaaactgaa gtgattcggc taatcgatga
     9841 acaagggaaa ctgactcccg agttagaagc agaaatcacc caagccgtga aaatgcaaca
     9901 agtggaagat ttgtatcgtc catacaaaca aaaacgacgg acaaaagcaa cgattgctaa
     9961 agaaaaagga cttgaaccac tcgcgaagtg gctgatgcag cttacagatg gagaggtgca
    10021 atctgaagca gaaaaatata ttgacaaaga aaaagaagtc tcttctgccg aagaagcgtt
    10081 gcacggtgct catgagatta ttgctgaaca agtcagcgat aatgcaaaat ttcggacttg
    10141 gattcggtct tatacgtata acaaaggtat gtatgtcagc aatgtcaagg atgagcaagc
    10201 agatgaaaaa ggcgtatatg aaatgtatta tgactttgca gaacccgtcc ataaaatggt
    10261 ttctcatcga atattagcta ctaatcgtgg agaaaaagaa gatattctga aagtattctt
    10321 tcaagtagat gaagcagcaa ttttagctta cttggatcgc caaatagtca aaaatcctgc
    10381 ctctccttct tcttcattcg tcagagaagc ctatcaggat agttataaac gctttattca
    10441 gccagcaatc gagcgagaac tgcgcaatga attgactgaa aaggcagatg agcaagcaat
    10501 cgctattttc ggcgaaaacc tccgtaatct tttgttgcaa ccgccactaa aaggtaaagt
    10561 tgtcctggga tttgatccgg cttatcgtac tggatgtaaa ctagcagtcg tagatgcgac
    10621 agggaaagtt ttagcaatcg aagttattta tcctcataaa cctgcagcgc aagcaaaaag
    10681 agaagctgct ggaccagctt ttatccaatt aattaataaa tatcaagtag atatggtagc
    10741 tatcggaaat ggtacagcca gtcgggaatc tgaacttttt gtggctgagc agttgaaatc
    10801 cgccgaccat aaaacctatt acgctatcgt caacgaagca ggcgcttctg tttactccgc
    10861 tagcgaaatt gcacgaaaag aatttccgca tctacaagtt gaagaaagaa gtgctgtttc
    10921 tattgcccgt cgcctgcaag atcctttagc tgaattagtc aaaatcgatc ctaaagctgt
    10981 cggagtaggc cagtaccaac atgacgtttc tcaaaaacgt ttggctgaac aattggattt
    11041 tgttgtggaa acagcggtta accaagtagg tgtggatgtg aatacagcaa gcccgcagct
    11101 cttgcagcat atatcaggat tgaataaaac aactgcccaa aacattgtca tttatcgtga
    11161 agaaaacgga gaattcaccg cacgaactca attgaaaaaa gtgccacgct taggaccaaa
    11221 agcatatgaa caagcaatcg gattcttgcg agtccctggt gggaaaaaca ttttggacaa
    11281 cacaggtatc catcccgaaa gttattctat tgcaaaagaa atattaactt cagtccaact
    11341 ttcagaaaaa gagcttggta cagaagaagc agtagaaaaa ttgacacgat tatccgtaga
    11401 gaaactggct gaaagcctat cagtcggaga agaaacgtta gccgatattt tagctgggct
    11461 gacacaacct ggacgagata tgcgtgatga gatgccagcg cccttattac gtacagatgt
    11521 attgagtatg gaagacttga aaccagggat ggaattgact ggaacggtcc gtaatgtcat
    11581 tgacttcggc gcatttgttg atatcggtgt gaagcaagac ggacttgtcc atatatcgaa
    11641 actcagcaaa aaattcgtca agcatcctac agatgtcgtg tcagtaggag atatcgtaac
    11701 agtttggata gaacaagtcg atacgaaaaa aggcagaatc agtttgacga tgctttcccc
    11761 ttatgaagag taacaatgag gagtaacaaa aattgacaca agaagaactg cagcagttag
    11821 tagaagagat atcattaaaa agcttccagc ttcctttcaa gcaccaagca atgttcaatc
    11881 cacgcttacg tacaactggc gggcgctatc atttaaacga tcatcattta gattttaatg
    11941 cgaagctatt tgaagagtca gacgaagaga cgattgcagg aatcatcaaa catgaacttt
    12001 gccattatca tcttcatcta gctggaagag ggtatcagca taaagatatg gatttcaagt
    12061 atcttttgaa aaaaacagga gggttgcgct atgcaccagc cttgacgaag aaacagataa
    12121 agatggagta ttatcgatgc caagattgtt ctgccacgat ctgccgcaag agaagaatcg
    12181 atgtacgcaa atatcgttgt ggaagatgtc atggagaaat caaatggatt gagacaaaaa
    12241 gagaagactt taatgaagca caaaaaatca gatgaaagga tagaaggcct ttggaaaaaa
    12301 gtgagcagag gaatctaaaa gactggaatc agctattaaa ttttttagcc gcacctgaag
    12361 agggaaattt tgaggaactg acggcaccta cacttatttt agctggaaac tgtcttccta
    12421 tcttagctga taaagcagca caaatgtatt taaatggcca agtagatcaa ttgtttttgg
    12481 tcggaggggt ggggcatgcg actagaatcc tttatgagaa ttttgaaaag caaggatttc
    12541 attttgaaga tggcatgagt gaaagtgaaa tctgtcgtca gtatctaaaa gaagtctatg
    12601 atttgcccga taaggctttt ctgatagaat cgaagtctac aaattctgga gagaacgcca
    12661 ctttttcact ggagattctt cattccttag gagctgtacc agaaaaaatc ttattgatga
    12721 atgatccgac actgcaacgc aggactcgag caacttttga gaaagtatgg caaaatgaac
    12781 aaactatttt cgcgaatgct gtgccatttg tgccggaaat ccttcacttt tctgaagaga
    12841 tcatctttac agcaaaagaa ctgaatcatc aatggcctaa ggaatatttc catgcgctag
    12901 tattaggaga aatggaacgt ctccatgacg atgagaacgg ctatggtccg aaaggaaaag
    12961 attttattcc tcacattgat atatcagagg aagtttggtc ttcttacacc agaattaaag
    13021 agtcaataaa aacggatttt tctcgtactt gaccatttta gcaagcgaat cctcctttcc
    13081 aaataaattt ggttgaaatt ttcaatcgtt tataagataa tgaggagcgt gttgaatgta
    13141 atagctaaaa gaatggacgc tagataagag aagaggattc aaatggaaaa tcaaaataaa
    13201 ttgtatcatt ttgttggtat caaaggttca ggaatgagct ccttggctct tgttttacat
    13261 gaacaaggtc taaatgtcca aggatcagac attgaaaaat atttctttac acaaagagat
    13321 ttagaaaaag caaatatcac gattctgcca tttaacgcag ataatgtaaa accagggatg
    13381 acgatcattg caggaaatgc atttcctgat tcacatgaag aaatccagcg agcaaaagaa
    13441 ttaggtttgg aagtgattcg ctatcacgac tttattggtc attttatcca aaattatacg
    13501 agtatcgctg taacaggctc tcacggaaaa acaagtacga ctggactgct ctctcatgta
    13561 ttgagtggtg tccgtccaac aagttatcta attggagatg gaacaggaca tggtgatccg
    13621 caagcggaat tcttttcatt tgaggcctgt gaatatcgcc gtcatttcct tgcctattca
    13681 ccagactatg caatcatgac caatatcgat tttgatcatc cggattatta tacaagtatc
    13741 gacgatgtat ttacagcttt ccaaacaatg gctggacaag tgaaaaaagc tatttttgcg
    13801 tatggtgacg atgcttattt acgcaaatta aaagcaaatg tgccgattta ttattatggt
    13861 gtcacagaaa acgatgatat ccaagcacga aacatcgaac gcacaacttc tggttctgct
    13921 tttgatgtct atcatgggga tgaatttgtt gggcacttca ctgtaccagc attcggtaaa
    13981 cacaatatct taaatgcttt aggtgttatt gcagtagctt atttcgaaaa actggatctg
    14041 aaagaagttg cagaagaaat gctgactttc ccaggagtaa aacgtcgatt cagtgaaaag
    14101 atcgtagcag acatgaccgt agtcgatgat tatgcgcatc accctgcaga gatcaaagca
    14161 acgattgatg gcgcacgcca aaaatatcca gataaagaaa taattgcagt tttccaaccc
    14221 catacgttta cgcgtacgat tgcattgatg gatgaatttg cagaagcact tgatttagct
    14281 gataaagttt acttatgcga tatttttggt tcggctcggg aagaacaagg aaacgttaag
    14341 attgaagacc ttggagcgaa aatcaaaaaa ggtggagaag tcatcaaaga aaataatgta
    14401 tctccattgt tagattatca tgatgcagta gtaatcttca tgggtgctgg agatgttcaa
    14461 aaatttgaac aagcgtatga aaaactttta agcagcacga cgaaaaatgt actgtaatac
    14521 aattactgca gtgtccttat aaaaatagaa agagggggag tggcaaaaat cttgccaagc
    14581 cccctttttt gcaacttatg ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc
    14641 caatcaaatc aaaaacatga gaagctgttt tttcttggct gttcttatta tttgatgcag
    14701 gacagctttt tcacgacctc tgtaaactat aggaatctgt taaatcaagg gagcagaact
    14761 tgccttttac gctacttttg gtacaataat gtatgattaa aaataaactg gagggtttct
    14821 atcttgaaca taggtaagcc gagaaaacta ggaaaaacaa taaatgaaat cgaggaaggc
    14881 gactcgttat ccttaacaga atctattgaa gataaccagc ttttgctgta tttgggattg
    14941 acgaatgatg caaatccttt gtatatccaa catgactttg cacaaaaaac agaatatcaa
    15001 cagccaatcg ttccatcagt gatgctgatg gggatcatca caagtgcaat ttcaaagcat
    15061 cttcctggac caggatcaca tgttgtcaat ttttctgtca attttattga accggttttc
    15121 cattatgaaa cactgacttt tcagtttgag gtcataaaag tagataagat gaaggatgtg
    15181 gtgacgattt ctgtagaagc ggagaatacg aacgaagatc gtgtgttgga tgctgtcgtt
    15241 atggtccaac cgccacaagt tcctgaattg gaaatagcaa tcgaaagtga aggtgaattg
    15301 aatgaataat ccaagtacat caatgtcagg tatcaatcgt ttttatgcaa aagtttatgg
    15361 atttttaggt atgggacttg cccttagtgc agtcacttct tacttggctc tttgggtatt
    15421 tcctttgcaa ttagcaactt ttatcaataa ttttccacta ggctttgtgg gtctgtggat
    15481 cgttgaactg attttagtaa tggttctagg cgtaaaagca caaaagaatc cgagcttagc
    15541 aatcggtgga tttattgcct actctctttt gaatggcata acattagcgg ttactcttgc
    15601 atactacgat attggaacag taacgcaagc tttcttgaca gctactttga tgttcatggg
    15661 gatgtctgtt gtcggagtaa caacaaaaag agatcttagc ggggttggaa gagcaggaca
    15721 ggcagcttta atcggagtga tcattgcact agtcttgaac atcttcattt tgagaagtag
    15781 cgtagttgag ctgttcatct ccctactgtt agtcgtcatc tttgcaggga ttacagctta
    15841 tgataaccaa agcatccgta atatctatgt ccagacaaac ggaacagcac atagtggaat
    15901 tgctgtattc atggcattga agctgtattt ggactttatc aacttgttct tggcgtttct
    15961 gagaatcttt ggacggaaca actaaaaaag aaaaaaattc atcagttggg gctataccga
    16021 attatttggt atggtcccgc tttttctttt cgcattccgc aggattcttt gttagaatga
    16081 ataagaactt tacagtaagg agttaagaag atgacaaaaa ataaattatt actcgttgat
    16141 gggaacagtg tggcttttcg cgcattcttt gcgcttcaca attctcttga acgtttcaaa
    16201 aatagaaacg gtcttcatac aaatgcaatc tatgcattca atacgatgtt tgaaaatgtt
    16261 atgcaaaaag agcaaccgac tcatgttctg gttgcgttcg atgcaggaaa aacgactttt
    16321 cggacagaaa tgtatgcgga gtacaaaggt ggaagatcca aaacacctgg cgaattcaaa
    16381 gaacaaatgc catatatacg tgatttgttg acaggactag gggtacagta ttacgaattg
    16441 cccaattatg aagcagatga tattatcggt actttggcag aaaaagtaga caaagatcaa
    16501 tttgatgtcg tcgtgctttc aggtgatcgg gatttgactc aattagctag taaagaagta
    16561 aaagtggata tcactgtaaa aggtgtcagc gatatcgaaa gctatacacc agaacatgta
    16621 gcggaaaaat atgatgggtt gacgccaaaa cagattattg acatgaaggg actagccgga
    16681 gatgcctctg acaatatccc cggtgtgact aaaatcggtg aaaaaacagc tatcaagcta
    16741 ttgaaacaat atggctcagt cgaaggaatt tatgaacata tcgatgaaat gaagcaaagc
    16801 aaaatgaaag aaaatctgat aaatgacaaa gagcaggctt tcttatcgaa aaaattggcg
    16861 accatcaata cttcagcgcc tgtggatgtc tctatcgact cattgaagta tgaagggaaa
    16921 aatctcgata aactcgttcc gttttataaa gaaatggatt tcaatcagtt cctttctaaa
    16981 ttgaatatcg tagaagaaga agtaaaaatg gatgatattt tgtttgaagt cgttcatgaa
    17041 ttcaaagagg aaatgtttac tacggatatg gcgttgtacg tggaaatgat gggcgacaat
    17101 tatcatacag aggaaatcgt cggagtagcg tgggggacag aaaagaaaat ttatgtcacg
    17161 aacgatctat ctgtattcga aaaccgagcg ttccattctt ggatcactga tccgactcgg
    17221 ctgaaaaaag tatatgatgc gaaacgaact tatgttgctt taaaccgcta tactggaaaa
    17281 acgaaaggaa tcgattttga tgtcttgcta ggtgcatacc ttctcgatac gaatgaaaaa
    17341 agcacagata tcgaaggaat agctgctcat tacggataca acgatatcca atcagatgaa
    17401 gctgtatacg gcaaaggggc gaaaaaaggt ttaccagaag aagaggaact cttttttgct
    17461 catttagcgc gaaaagtagc tgcaatcaat gcgttgacac caaaattgag ccaagaactg
    17521 gcagaaaaaa accaagaaga tcttttccgg aaaatggaac tgccattgtc tatcattttg
    17581 gcagagatgg aaataagcgg gatcaaagta gatgcgactc gcttacaaga aatgaagggc
    17641 gagttctctg ctcgcttaag agagatcgaa caaaaaatct acgaagaagc cggcgaagaa
    17701 ttcaatttga attcaccaaa gcaattagga gtgatcttat ttgaaaaaat gggattaccc
    17761 gttatcaaaa aaacaaaaac tggatattcc acagccgtag atgtactgga acagcttcgt
    17821 gaacaagcac caatcgtaga ggatatcctc acctatcgtc aaattgcaaa aattcaatct
    17881 acctatgtcg agggactgct gaaagtgatc caatcagatg gaaaagtgca tacccgttat
    17941 gtccagacat tgacccaaac aggacgatta agctctgttg atcctaattt gcagaacatc
    18001 ccgattcgat tagatgaagg gagaaagata cgtcaggcat ttgttcctag agaaaaagac
    18061 tggctgattt attcttctga ctattcacaa atcgagttac gcgtacttgc gcatatctca
    18121 aatgatgaac atttgaaagc tgccttccta gaaggtcaag atatccatgc cagcacagcg
    18181 atgcgtgtat ttggaatcga aaaggcagag gatgtcacac caaatatgcg taggcaggca
    18241 aaagcagtga attttggtat cgtgtatggc atctctgatt acggactttc acaaaatcta
    18301 ggaatcagcc gaaaagcagc acagcaatac atcgatacgt actttgaaaa atacccaggt
    18361 gtcaaagaat atatggaaag aatcgtacgc gaagcaaaag accaaggcta cgtcgaaacg
    18421 ctgtatcacc gccgtcgata tctgccagac atcaattcaa gaaattacaa tatccgttct
    18481 tttgcggaac gaacagctat caatacacca atccaaggaa gtgctgctga tattttgaaa
    18541 atcgcgatga tcgaattgga taaacgatta aaagaaacag gtcttcaagc tacgatgctg
    18601 ttacaagttc atgatgaact tgtcttcgag gtaccggaaa aagaacttga atcattagat
    18661 aaattggtga aggaagttat ggaacaagct gtttctcttc atgtaccact gatcactgac
    18721 agcagttggg gaaaaacgtg gtacgaagca aaataaacat tgtttaagac agagcaatat
    18781 aatgaaggtg aaaagaaaaa atgccagaat taccagaagt agaaaccgtt cgtaaaggat
    18841 tggaacggtt agtagtagga aaaacgattc aaaaagttca ggtcttgtgg cccaagatca
    18901 tcgagcaacc agagacaccc atctttgagg cttctcttgt tggcgaaacc atccaatcca
    18961 ttggacgtag agggaagttt ttaatttttc atctcgatca ttgtgaactg atctcacact
    19021 tgcgaatgga aggaaaatat caatttacga aagaaaatac accaatcgat aaacatacgc
    19081 atgtactttt cttttttgag gatggaagcc aacttcgcta caacgatgta cgaaaatttg
    19141 gtcgtatgac aatcgtcgaa aaaggagcaa gtgcaactta ccgaggaatc atgaaattgg
    19201 gacctgagcc tttgccagat agcttcttat tagcagattt cgcaaatgga ctgaaaaagt
    19261 cccacaaagc aatcaagcca ttgctgctag accaacgtct agtgactggt ctaggtaata
    19321 tttatgtaga tgaagcatta tgggaagcga agatccatcc agaacaaccg gcgaacacac
    19381 tgcgccccaa agaggtagaa caattacgtc tatcgatcat tgatgttttg gatcgtgcaa
    19441 tagaagccgg aggtaccacg attcgcagtt atttgaatgc attaggagaa tcaggcggat
    19501 ttcaagtagc actgcatgtc tatcagcaaa ctggcaaacc atgtatacgt tgtggaacgc
    19561 cgatcgtgaa aacaaaagtg gcacaaaggg ggacgcacta ttgtccgaat tgtcaaaggc
    19621 taaaaatcgc aaagtaggat ttgttttagg tttgacgggt ggaatagcta ccggtaaaag
    19681 tacggcagcc aaagtctttc agtctcatgg atttcctttg atcgatggcg atgtgattgc
    19741 cagagaaact gttgagcctg gaacgcttgc tctgaggaaa attgtatcag tgtttggcca
    19801 agaggtattg cagcctgatg ggcaactaga tcgagggaaa cttgggatga ttgtttttcc
    19861 atcaaaggaa aaacggcaaa aacttgatca gctattagat ccgtttatcc gaaaagcgat
    19921 aaaagatcaa atcgcctctc taagttccaa tcatccttta gtaattgtag atatcccatt
    19981 gctctacgaa ggtcattatg accactatat ggatgcagta gctgtcgttt atactacgcc
    20041 agaaactcag cttcagcgtt tgatgaaacg taatcaactg acgctagaac aagcgcagca
    20101 gagaattagt agtcaacttc caatcgaaga aaaaaagcag cgtgcggata ttttgttcga
    20161 caacaatgga acaaaagaaa aacttgtggg acaaatcgaa aattggcttt ttgctaatcg
    20221 ctttctttga gtatctgtgt caattttgct atcctgagta tatagaaagt gagggatagc
    20281 gatgggatca ttaaaaaatc aaaaggataa agtcgtaggt aacgcaaaag aaaaaatcgg
    20341 cgagatgctc aataaagaaa atctaatcga tgaaggtcgt acgcaatcag aaacagcaga
    20401 agaaaaagaa aagcagtacc aagcaagaat cgatgaaata gaagaccagc aggcagaaga
    20461 agaagcgaag cggcaagatt tgactcaaga acgaaaaaat ccgacacctg atcgtagtaa
    20521 aacaccgctc ccagaaagtg accacattcc cgatgagcaa aaaatcgagg aacagcgaat
    20581 gaaacactat acaggaatcg aagagaagtt ataaaatagt aaaaaatcga gatgtgactt
    20641 gaagcagatg agaaggtgtt catctgcttc aagttttttg tgctagatga ctaaatcagc
    20701 ttttgtgtaa gttatttctc agtaatcaag gtgatcagta ttggaaaatt gaaatttagt
    20761 aaggacaggg gacacaatta cgcatgaaag tagaaaaaat tgttcttata tccgtgtttt
    20821 ttgggtagga cagtcgcgtt tgtgatttgt ttcgtgttat aatacaacta gttgtaaaga
    20881 aagacacatc ctttatataa aatgcgatgc aagaggtgag acaaaatgag atgtcccaga
    20941 tgtcaacata ataattcacg agtaatcgac agccgtcaag cagatgatgg acgtgcgatc
    21001 cgtcgccgtc gcgaatgtga gaattgttcg ttccgattca ctacatttga acgaattgaa
    21061 gctgctccgt tattagtaat caagaaaaac ggggaacgcg aagagttcaa tcgcgataaa
    21121 attttaagag gactcatccg ttcagcagaa aaaagacctg tagctatgga acaaatggaa
    21181 caaatcgtca accatgtcga aaaccgcgta cgcagtctcg gagaaaacga agtatcgacg
    21241 aatctaattg gtgaatatgt gatggaagat ttagttgatt tagacgaaat cgcttatatt
    21301 cgttttgcaa gtgtttatcg ccagttcaaa gatatgtctg ttttcttaaa agagttgcaa
    21361 gatatcgtgg ataaagcaaa agaaaataat aaagagaata ataaagaaaa caattaagaa
    21421 acaaaaagga ggcatttcct tggaaaatgc ttggaaagag cttcaaccaa aaaatattta
    21481 ccaaggaaga aaagcggctc cgctgagaga agacagcaag aacgccttgc tgtatcttta
    21541 tcagccacta atcggcggag aagccttgag tatctatttc accctgttat cagaaatctc
    21601 tcaagaatct ggcaaaggtc ctgaagggat gcatgcagat ctgcttagta gtctaggctg
    21661 cggtatcccg caattttatg atgcaagaaa aaagctagaa ggaatcggct tactggatgt
    21721 ttattttaaa gaagatagtg atctgggacc tcgttttatt tatgagctgt tagaaccact
    21781 cattcctaaa gcatttttca aagactctgt tctggctttt cttcttctgg aaaaagttgg
    21841 tgaacgacga ttccagaagc ttacccaatt ctttgaacct aaaaggcata cagcagaggg
    21901 atatcaaaaa atcacaaaaa aatttttaga tgtttatcgc ttcaatgaag cagtgtacgc
    21961 tgctaatcaa gaaaacatcg aatcgctgaa taaatctttt gaagaatctg aatcagtagc
    22021 attacttgaa gcagattcga cattcgattg gcagtttttg aatgattggc tggcaaaaaa
    22081 acatattgca agtcttgatg atccaatcat caaacaagtc gacatgtatc atcagcttta
    22141 cggtttcgat gaattgacac ttggcgaact gatcagtcaa tcttatgact ttagtgaaca
    22201 aaaagtatct gaaaaagaac tgcaaaaagc cattttggca atgcagagac gtccggtgaa
    22261 gcaaacaaaa gaaacagttg cggtttctga aaacagtaat gaaccctcgt caaacgaaca
    22321 tttgacaaac gcagcgcttc agctgataaa agaagcacag agcattccac cgatgaaata
    22381 tctggaagca atcaagtcag aaaaaggcgg atatgtttct aaacaagaaa actggttact
    22441 gcaagatctt gtttctcaat caggtctttc cagtagcgtg atcaatgttt tgctgaatta
    22501 tgtactagtg atcaagaata atgctagctt aaatgccagt tatgtaaata cagtggcaaa
    22561 tgaatgggca caaaagaaaa tcgccacagc agaagaagcg atccagcaca ttcggcaaat
    22621 aagtaatcaa gcaaaacaat caaaacaaaa gaagcaaaca aattatcaaa ctggcaagcg
    22681 aaatgttcga cgggaaaagc ttcctgattg ggtcaaccag ccaaaagatg aaaaacagat
    22741 cagcagcgag aaaaaagcag agattgaccg ccgttttaaa gaatacctgg caaaaaagga
    22801 gggtgatatc tagtggaaga cgtaggcaaa gaattgaaga ggatcatcag caaacgcaat
    22861 tatcaagaaa agtatgctga aatgatcgct gaagtcttaa atgatgcgga tgttcaagct
    22921 tttctaagcg cacaccaaga agaattgaca caagcggata ttgaaaaaag ttacgctaaa
    22981 ttgtatgaat tcgtgcaaga aaagcgtaaa tatcaaaaaa acgatccaac gatgatcgct
    23041 cccggctatg agccgaagct gacgttgaat tttcattatg tggatgtcac ttatgtacca
    23101 acggaagcat tgttagcacg gcaaagacaa gaagaaatca gaagccgagt aaaagcaatg
    23161 gatatgccaa aagatattca atcggcttct tttgaaaact ttgaacgtac agatggacga
    23221 ggatatgcga gcttagaagc cctagatttt gtagagaaat atactgctga tccaaaaggg
    23281 tatcataaag gactgtatct agtaggaagt tttggtattg gaaaaacata tttattagga
    23341 gcaacagcga gagaattagc gattgctgga ttcaccacga cgctagtcca ttttccgaca
    23401 tttgccgttg agatgaaaca ggcaatcgga aaagatcaag tggcagaaaa gttagatgca
    23461 gtgaaacgtt cacctatttt gatgatcgat gacattggtg cagatgcgat gagtagttgg
    23521 atacgagacg aagtattcgg tgttatcctt caatatcgga tgcaggaaca gttaccaacc
    23581 ttcttctcat cgaattttac aatggatgaa ttggaacagc atttgtccgt cactcaacgt
    23641 ggagatgaag agccgctaaa agcgaaacgg ataatggaac gcatacgtta tttgacaaaa
    23701 gaaatcgaaa tgactggtcg caaccgacgc aatggttgaa cagttgacaa gtccaactga
    23761 catttgacct agtagtaaaa gcaggtcagc gaatcattat atggacagaa atgaccggag
    23821 acaatcaccg caacaagtaa tgcagaaact taaacggagg attttttatg agggctattt
    23881 tttctagtat atctgtagtt ggtctatttt ttcttttagc aggctgcggt agcgcagata
    23941 gcaacacagg ttctgttgat tcgattgatg cgggtcaaac agtaaaaaca agcagtacga
    24001 aaacggagag acaaagcata tttgtcggaa cgattgaaag tatcactcaa gtagaagcag
    24061 aagaagaaac gcttcagttg aaactcgtcg atgtcatggc caaagaagat ccagcgaata
    24121 tgggaacgtc attcagcaat gacggagtga tcttaaatgc tactgctgaa aaaataagcg
    24181 tggacataaa tgaattgaaa atcggtgaca aagtccaatt tacattagtt gagatgccga
    24241 tcatgacgat gtctatccca ccgcaagttc ctggagattc gattattgat gtttcattga
    24301 acaaataacc aataacattt tttcaaaaat aagaaaatgt tgttttatag aacgaaaagg
    24361 tatatttttt aaggagatat gggaattttt ttattcctag tctccttatt ttatttggaa
    24421 aatgctataa ttatctagaa ggagtgaagc tatgaaccat ttagatttat ctcagcgctt
    24481 agccgcagtg ggtgactttg tccctgaatc cgctcgttta gcagacattg gatcagatca
    24541 cgcttactta cccgttgcat tgatattaag aggaaaaatc gaatatgcag ttgctggtga
    24601 agtcgttcaa ggtccttatg aatcagcacg taagcaagtc aaaaaagatg gattagaaga
    24661 ccgtatcacc gtacgccttg caaatggtct agatgctatc cagccacaag atggtattac
    24721 agctgtcacg attgcgggta tgggaggttc actgatccga gatatcctag aagctggcaa
    24781 gttgaatcaa cgcttaagcg gacaagaacg tttgatcctt cagccaaaca tcggagagcg
    24841 tacattacga tcttggctgc aagaaaatca gtataaaatc caatcagaaa aaatacttga
    24901 agaaaataaa aaaatatatg aaatcattgt agccgaaaaa gaacaaaaac cagttactta
    24961 caatgaaatg gagctgatgt tcgggccatt tctgcttgaa gagaaaagtg agatcttcaa
    25021 atcaaaatgg acaagagagc ttcaacaaag aaaagctatc cgtgaacagc tcaaacaagc
    25081 agctaatcaa gaggcacgtc aaaaacaaat cgagcaggaa atccaaatga ttgaggaggt
    25141 tttaaagcca tgacaatcag cggacgcact ttcatccaaa agtttgaaag ctattgtcct
    25201 cagtggctag ctgaagaagg agacccagtc ggactgcata tcggaacatt ggataagcca
    25261 atccaacgag taatgatgac attagacgtt cgtccagaag tggtcgaaga agcaatcaag
    25321 aaaaaaatcg atttgctgat tgctaaacat cctccaatct ttcgtcctgt aaaacggctg
    25381 gttactgatc agccacaaga aaaaatgtat gcagatttgt tgaaacatga tattgccgtc
    25441 tatgcagcac acacgaacat ggatattatt tgggatggac tgaatgactg gttttgtgaa
    25501 cttctcggta ttgaagtgga gagttattta gtaaagactc atgagattca ttataaaaaa
    25561 ctggccgttt atgtacctgt ggatcatgca cagaaaatgc gtgaagtatt agctgcagct
    25621 ggtgctggta cacaaggtga ttataccggt acaagtttta cctcgattgg acacggtcga
    25681 tttactccgg aagctggtgc acaaccagcg attgggaaag tagggaagac agaacaagtt
    25741 caagaggcaa aagttgaggt tattttgccg gaaactatcg aaaaacaagt gattcaagcc
    25801 atgcggtcag ctcatcctta tgaggaacct gcttatgatc ttttcgctat agatgaacca
    25861 gtcgaaatgt ttgggttagg acgagtaggt gaattgccgc aagaaatttc tatagaggca
    25921 tttgtagaac aagtaaaaga agcgttccaa ctagatggac tgagaatagt ccagccaaaa
    25981 aatgcaaaat cctctgtgaa gcgcattgcg atttgcggtg gaagcggtga aaaattttat
    26041 ccgcaagcaa tcgcccaaag agcagatgtc tatatcacgg gagatatcta ttatcatacg
    26101 gctcatgata tgcaaagcgc tgggttgatt gcaattgatc ccggtcatta tattgaatcg
    26161 ttatgcaagc aacgatttat cgagaaattt gaatcttgga aacaagaaga aaattgggat
    26221 attaactttt tcgtttctga aacaaataca aacccatttc aatttaatta aaaggagaga
    26281 atactatgta cgaaaatctt ttgccacgtt ttttacgata tgtcaaaaca gaaacacgct
    26341 ccgatgcaac aagcaaaacg actccatcga ctcagacaca agttgctttt gcgcaagaat
    26401 tgaaaaaaga attagaagag ttaggcttag ctgatgtttt ctataatgaa gataacggat
    26461 tcgttatggg aacattacca agcaatgttg atcatgaagt gcgttccatt ggatttattt
    26521 cccacatgga tacagcagat tttaatgcag taggagtcaa tccacaaatc attgaaaact
    26581 atgatggcga gtctacgatc gtactagatg cggaagaacg ctatacattg aatacaaaag
    26641 attttccaaa tttgaaaaat tatgaaggcc acacattgat cacaacagat ggcagtacat
    26701 tactgggagc agatgacaaa tcagggattg cggaaatcat gactgcaatg gagatcttga
    26761 tcaataatcc ttctatccct cacggtacca tcaaagtggc ttttggtcct gatgaagaaa
    26821 tcggtgtcgg agcggataaa ttcgatgttg agcaattcga tgtagatttt gcttatacag
    26881 tcgatggtgg tcctttaggt gagttgcagt atgaaacgtt tagtgctgct caagcaaata
    26941 ttacgatcca agggaaaaat gtacatccag ggacggcaaa agatacaatg atcaatgctc
    27001 tacagttagc aatcgatttt cataatcagc ttccagccga tgaagttcca gaaaaaacgg
    27061 aaggatacga aggtttcttc catttgatgg cattggatgg gaatcctgaa gaagcttcta
    27121 tgagttatat cattcgcgat catgatcgtg aaaaattcga agcacgcaaa gcacaaatca
    27181 cacatatcca agaagagttg aatcagcgat ttgatcaaga acgaatcaaa gtagatatgt
    27241 atgatcaata ttacaacatg aaagaaatca ttgaaaaaga tatgagcatc gtggaattag
    27301 cgaaacaagc aatgatcgag cttggtatct ctccagtgat cgaaccagtt cgtggaggaa
    27361 cagatggttc aaaaatctct tacatgggta ttccgacgcc aaatctattt gccggaggag
    27421 aaaatatgca cggacgattc gaatttgttt ctttacaagt aatggaaaaa gcaacagacg
    27481 tgattgtaaa aattgcagaa ttgaacacaa agagttaaaa tcgaaaaaga acagcttatg
    27541 acaaggcgtt cgtcccaatg atggaatgtc atctgtctaa ggctaaaggg caggacaatg
    27601 gaattggttg tcccgccctc ttttttgaag agtaaatagg aggtaaagaa tggaaaagct
    27661 gctagttttt tataacaaaa gttcagggaa agatgagggt gagaaattag cacgctggtt
    27721 tcaagattat gtaaaagaaa agcagccaga attatatgta gaactagctg aaactggccc
    27781 atcgattgat gatgcggaat tagtcaagaa agcagaagcc atagaagctg atactttagt
    27841 aattataggt ggagacggca cgattcatca tatcgtccaa gcgttccaaa aaacattatc
    27901 tcaataccat gtcggattac ttccaggtgg aactgtcaac aaccttgcac gtgttcttga
    27961 gattcccctc aaaaaagaag aagctgctga cgtgatcctg gaaggcaatg tacggaaaat
    28021 agactatggt caagtcaatg atgaagtcat tatctctaca ctgacaatcg gaatcttagc
    28081 agatacagca gtttggatca gtcaagaaga aaaacagcgt tacggctcgt ggatctttat
    28141 caagaagttt tttaagttat tgctgaagaa gaaaaaatat catttggatt tagaaacaga
    28201 cgaggagcat tggaaaggaa aatcccaact gttgacagtc acgatgtcta actccgtggg
    28261 cggttttaca aattttgacg actcagcgac cctagatgat ggaaaatttc atgtaacgat
    28321 cgtaccttcc ttagatattt tccgatttat tttttatttg cctagaatca tgagaggcaa
    28381 attctatacg atccccggca ttacttattt cacagccaaa cgcatgaaaa tccaagcgca
    28441 ggaaaaatct gtgggcactc gaacggatgg agacacgaca gatgatcttc cagttgaatt
    28501 atctgttgta tcaaaaggat taacagttta ttcaccaaag aaagaagaaa aagcttagaa
    28561 atagtagtta acagaaagtt ggaagaagag taatggatgt attgttacac aatcctttgt
    28621 ggttttacct tttcattgtg aatttttact tattttgtct gatggggtat gataaacatc
    28681 aggctagaac tagcggctgg cgtgttcctg agaataattt gatatttgtc agcctgttag
    28741 gcggtggttt gggaggattg atctctcgaa aagttttcca tcataaaact cggaaaaaga
    28801 agtttactat aggattcgct gcaggtgtac ttgttgattt gattctcatt tattttttcc
    28861 attaaggagc aggagatgtc gtctaaagga acgaaaacta aaatcattct gaatctgctt
    28921 ttgctgattg tgatttttgc cattatttat tatttaatca atcaatcatt tgcagatatc
    28981 tttgccgaat tgctgtctac cagtgttgtt gttttggcag cggctgttat attagggaca
    29041 gtttaccaaa tcgctgaagg gcgctcaatc aaagaaattg ctgaaccttt cgcgccgaat
    29101 tttacaacga aagatgggtt cttgacctct agctatattg cgttttaccg aatcgtttcg
    29161 tttggtacag gaacgctgat ctcggagatt tatttttata acaaaaaagg aatgaagcta
    29221 tcccagggag caggggtgac agcactccat atgattatgt acaaactggc agtgatcact
    29281 tatgcattag ctggcttggt catccaattc tctctttttt attctaatgg tccatcaatg
    29341 atctggttta tcatcgctgg tatccttttg acgatcgtga ttgttgcctt tttattggcg
    29401 atgtccgtca gcttgaatgt tcaaatattt tttgtcagct tatcgaataa gttatttaaa
    29461 agtgagcgtt tgcggaaatg ggtggataca tgcaatcagc agatctactc tttaagggaa
    29521 acggtccaaa ccattatcca agacaggact gcgctattgc ggatttatct atggaatatg
    29581 ttaaaacttg tattttggta tgtacttcct tatttgattc tgattgaaaa ccatcctaaa
    29641 atagattttc tactgaccat ttcgtttatc agctttgcag tagtgctttc aggggttatt
    29701 cctacgcctg ctggtattgg ttcctttgaa tttgtctatt tgttattatt cagaccagtt
    29761 gttggaacag ttgacgcagt ttcttcgctt ttactgtatc gtttcagttc ctttatcttg
    29821 ccatttctct atggatttgt gtatgtgatt gctgaacggc gaagagtgat caaagaagaa
    29881 ataatagaag taaaaaaaga gaataaagaa aatcaagggt aggtctttta taagatctac
    29941 cctttttatc aaatcaaaga aaaacgttat ggataagata attttacgtt tgtttggctc
    30001 tttttttata tacatacaat caattttgtt tttttaagat ttatataagt cgagaaaaaa
    30061 agtcaaaaaa ctatgtgtat attttgacca aaattgacca aagcgatata atagtttttg
    30121 taaagatcaa agaaataagt gatcttacct ttaattagga gggtatacct gtatgaatat
    30181 tgagaaaatg actacgacgc ttcaagaagc gattgctgaa gctcaacaga ttgcggtcac
    30241 tcgaaaacat caagatatcg acattgccca tgtatggaaa atctttttac aatctaatca
    30301 ttttggtcga aatttctata cggatgccgg attggatgtc gaatcttttg aacatgaaat
    30361 tgatcgtcta ctggatgagt atccagttgt ttctggtgga aacgtccaat atggacaaaa
    30421 cctcagccag aatttctttc gattattgaa tgaagcagac caaatacgtg aaagcttcgg
    30481 cgacgagttt ctttcaacgg aagtcgttat tttagcgttg atgaaattaa aaaattatcc
    30541 gttaactgtt tatttgaata aaaacggatt atctgaaaaa gaattgcgta aaaacatcga
    30601 agagatgaga ggaggagaaa gagtgacctc acaaaaccaa gaagaacaat acaaagcatt
    30661 agaaaaatac ggcgttgatt tagtacagca agtcaaaagc ggaaaaatgg atccaatcat
    30721 cggtcgtgat gaagaaatcc gtgatgtcat ccgtattctt tcacgaaaaa caaaaaataa
    30781 tcctgttctg ataggagaac ccggtgtagg gaaaacggca atcgttgaag ggcttgctca
    30841 acgtatcgta cgcaaagatg tgcctgaaaa tttaaaagat aaaacgattt tttcattaga
    30901 tatgggtgca ttgatcgctg gagcaaaatt ccgtggtgaa tttgaagaaa gactaaaagc
    30961 agtcttgaaa gaagtcaaaa aaagtgatgg acgtatcctc ttgttcatcg atgaaatcca
    31021 caatatcgta ggggcaggaa aaacagaagg aagtatggat gccggaaatc tgttgaaacc
    31081 aatgcttgct cgcggtgaat tacatttgat cggtgccaca actttagatg agtaccgtca
    31141 atatatggaa aaagacaaag cgttggaacg tcgctttcaa aaggtattag tcaaagaacc
    31201 aacagtggaa gatacgatca gtattctgcg tggattaaaa gaacgttttg agatccacca
    31261 tggtgttaac atccatgaca acgcattagt agcagcagct actttatctg accgttatat
    31321 tactgatcgg ttcttaccgg acaaagcaat cgatttgatc gatgaagcca gtgcaacgat
    31381 tcgggtagaa atgaactcta tgccaacgga attagatcaa gttactagac gtttgatgca
    31441 gttggaaatc gaagaagctg cactgaaaaa agaatcagat gatgcaagta aaaaacgctt
    31501 gaaaaatttg caagaagaat tagctgaact tcgtgaagaa gcaaatgcga tgaagatgca
    31561 gtgggaaaca gaaaaagaag aagtcaattc tgtttcagca aaacgtgctg aaatcgataa
    31621 agcaaaacac gaattagaag atgcagaaaa taactatgat ttagaacgcg ctgctgtatt
    31681 gcgtcatggt acgatacctc aattagaaaa agaattgaag gaattagaag ctaaagcaaa
    31741 agacagcgag attaagatgg ttcaagagtc cgttaccgaa aatgagattg ctcaagtcgt
    31801 tggcagatta acgggtatcc ccgtaacgaa acttgtagaa ggtgaacgag aaaaactaat
    31861 taaattgaat gaaacacttc ataaacgagt gattggtcaa gatgaagcag ttgatgcggt
    31921 cagtgacgcg gtaattcgtt cacgggctgg attgcaagac ccaaatcgtc ctctaggttc
    31981 tttccttttc ctaggaccaa ctggtgtcgg aaaaaccgaa ttagcgaaag cgttagctga
    32041 gaacttgttt gactcagaag atcatatggt acggatcgat atgagtgaat atatggaaaa
    32101 acatgctgtg tctcgtctag tcggagctcc tccaggctat gtaggttacg aagaaggtgg
    32161 tcaattaacg gaagctgtca gacgaaatcc ttatacaatc gtcttgttgg atgaaatcga
    32221 aaaagcgcat cccgatgtct tcaatatctt gttacaagtc ctagatgacg gacgcttgac
    32281 tgattcaaaa gggcgagtag tagatttcaa aaatacggtt ctgatcatga ccagcaatat
    32341 cggttcacag ttactgctgg aaggcgtgac tgctgatgga acgattccag aagcagtggc
    32401 tgaacaagtc aacacgctat tacgtggaaa cttcaaacca gaattcttga accgaatcga
    32461 cgacacgatt ttatttacac cattgagcct agacaacgtg aaaggaatcg ttgacaaaat
    32521 ggtggcgcaa ttggcacaac gtctggaaca tcaagaaatc ctgctgacaa tcagtgatga
    32581 agccaaaacg tggattgctg aaaatgctta tgaaccagca tatggtgcaa gaccattgaa
    32641 acgatttatc actaaggaag tagaaacacc actagctaaa gaaatcgttg caggacatgt
    32701 tatgcctaaa tcaaaagtaa cgattacttt acttgatgga caacttcatt tcaaaacaga
    32761 agagctagaa gaaatcgtct aatcttccat tgagactata aaagaaaaaa ggggtctgac
    32821 acaactagtg tcagacctcc ttttttcaaa taaacagtgt tccagaagta gtttcttcga
    32881 aaaataagcc gaaatttctt gaagctgccc gcttctgtca caacctcttt tattaggtat
    32941 ttttcacttt ttctaattta taactttcaa agtaacgctc atctttttta ctttgagacc
    33001 atgcaaacaa tcccattaag gcagcaccta tcgtatttgt cagcaagtcg cccatcgtat
    33061 ccatcaatgc tgctcgtccg acaaacagtg tgccatcaga agcagcaaaa cgttgaagat
    33121 tcatgcctaa gaactggtcg cataagaatt cccagaactc ccaaaacacg ccgcaaagtc
    33181 cagcaaacgc aaagccgaat aagaggaaca accaaggact ggtttttgat atctcagcat
    33241 ctttcatcaa gtaaccaatc aatccatagc caagagctgt caggaccatt ggactcaccg
    33301 catgtaagat tttgtcccaa aaagagatga tactgatcaa atgcatccct gtgcctaaaa
    33361 agacagagat gaacaagaaa aaccagtaaa acaaaacgat tgctgctggt attttaagtt
    33421 tgaaaacttt caaaatgact tgtggcatga aaatcaatgc aatccctaat aaagattcca
    33481 cgataaatgt gattccttga gaagtcgaat atttttgtgt gataaattca taaaaattat
    33541 agcagacaac gaatacacca aaagcaagta aagctatttg cacctttttg atttctttaa
    33601 tcactgaaag tccctccatc taaaaatctt tttacttctg aaagcaactc aatttctgaa
    33661 gaaaccactt ttccgttcaa tttgatcaaa cctattgtat ataagttcag gtagtgaaat
    33721 tgattttcag ctgtttcttg tagtgcagcg atttttttcg ggtgggttcc gccttgctgt
    33781 cgagtatcag tatataagcc gatcactggg atgcctttcg cataagccac accgatttcc
    33841 gatgctaccc cggcatcgat cgtcagccca tctagtaaag caatcattaa atcactagcc
    33901 aacacttttt ctgtgtcagc aagagcaatc attcgactgt ctgcataagc agttttgtca
    33961 ttgattgctt cattttcttg aggaagatag atcgaaagct cagaagagag ctctctgatt
    34021 tctttaacta actgttggtt atataacaaa tcacttttag caaacattgg tgcggcaaaa
    34081 taaattttca tctaaacatc atccttttta tcaaaaataa tcgctaaagt aagcttccat
    34141 gtttggaatg atacgctcta gtgacttcag tgtttcttca tctgtttcta accgttcgat
    34201 tcttgccaaa taagaagcac ggcgataatt catcatcaag caagtcaatg tttggatatc
    34261 cagtcgaacg gattttccta taggttcatt taagacacgg acttgtccct gttcatccca
    34321 tgttaaagcg aagacaccat tattccaagg agcgacagga tcttcaatga tgaaatgaaa
    34381 agcatcagct gttccaacaa acggaaaccg ttgaaggaat tcttttacat caacgatccg
    34441 agccataaaa tatggttcga tctgctcttt gatctgacta tcttcaatca aaaatgctaa
    34501 aggctcattt ttataaatat ctccatgtac ccagtagatc atggaaaaat gtgcggaaat
    34561 aaagttccat aagccatttc gagcttcttg atcaagatag aacatttctt ttacgtgaaa
    34621 tatttcttca gctacccagt agaacagaac acctcttggt tcatggttgc tatcgtagta
    34681 gacagcggct gtgcgctcat cttcattttc gaaacgccaa tactcttccc aatgaaaatc
    34741 actgcggaat aaagcaccat ggttttgttg ggcaaatttg tcataaacgg aaaatacgtc
    34801 tggatgatca actggtaatc gctcaaccat accacttact gggatttgtt taggtagctg
    34861 tgtatctctg attttaaagg aaagtttatc agacatgatt tcccatcctt tacgccgata
    34921 gtaggggata ctgtaaggat acaaataaga gatccattgc ttattcttgc gcatattatc
    34981 caatgcaacg ataattagat cttgcatcaa tccatggtta gcatattctg gataagttcc
    35041 aactccagtc acaccaccca ttttataacg tgcaccgtga atattgactt cacaaggata
    35101 gatagcgatt tgagagatca gtttattttc gtggaaccat ccgaacactt tcgatacttc
    35161 caaaatcggt tttttggaac ggataaaagc tcgtttattc tcaaacccgc tttcctcgat
    35221 gtctgcttca gtcacttgga acacatagga aagcaattca ttgaattgat cgatatgttc
    35281 ttgagtgacc ggttttaatt caagattttc tctaaaatct ttttgatcca ttttttcatc
    35341 tccatttatt agcttgataa cagtatagca aaattctaca aaaaaaactt ctatagctga
    35401 ctaattaaaa actctttaaa ttactgcttt tcgttggtat aatggaaagt gctgattata
    35461 tgaaaagagg agacgctatg gatataaaga aaatgaaaga acgccaagag cagattcgta
    35521 atttttcgat catcgctcat atcgaccatg ggaaatctac attggccgat cgtattttag
    35581 aaatgacgca cactgtgacg tctcgcgaaa tgcaagatca gctgttagac tcgatggatt
    35641 tggaacgtga acgtggaatc acgattaaat taaatgctgt agaactcaat tacacagcga
    35701 aaaatggaga aacatatatc tttcacttga ttgacacccc agggcacgtc gacttcacct
    35761 atgaagtctc tcgaagcctt gctgcctgtg agggtgccgt tttagtggtc gatgcagcac
    35821 aaggaatcga agcacaaaca ttggccaatg tttatttagc gttagacaat gatttggaga
    35881 tcctgccagt catcaataaa atcgacctac ccgcagcaga tcccgaacgt gtccgtcaag
    35941 aaatcgaaga cgtaatcgga atcgatgcta gtgaagctgt attagccagt gccaaatcag
    36001 ggattgggat tgaagatatt ctcgagcaga tcgtggaata tgttcctgca ccaagtggag
    36061 acttagatgc accgctaaaa gctttgatct ttgactctat ttatgatagt taccgcggtg
    36121 ttgttctaaa tgtccggatc acggacggta tggtcaaacc cggagacaaa atcaaactga
    36181 tgagtaatgg caaaacattt gatgttacag aggtcggggt cttctcgcca aaagcagttg
    36241 ctagagactt tttgatggtc ggtgatgtcg gttatatcac agcaagtatc aaaactgtcc
    36301 aagatacacg agtaggggat acagtaacgt tagcagacaa tcctgccgct gaagctttac
    36361 caggttaccg aaaaatgaat ccgatggttt attgtggtct ttatccaatt gatacttctc
    36421 gttacaacga tttgcgggaa gctttagaaa aactgcaatt gaatgatgct gctttacaat
    36481 tcgaaccaga aacctctcaa gcattaggtt ttgggttccg ctgtggattc ctcggacttc
    36541 ttcatatgga tgtcgtccaa gaacgcttgg aacgggaatt caatctagaa ttgatcacga
    36601 ctgcaccatc cgttatttac catgttaata aaacggatgg tacgactgct acagtagata
    36661 acccggcaga ttttcctgaa cctgtaacga ttcaggatgt agaagaacca ttcgtcaaag
    36721 cacagatcat ggtcccaaat gattttgtcg gtgcagtaat ggaactttct caacgaaaac
    36781 ggggagaatt cattacgatg gattatttag atgattaccg agtaaatgtc gtctataata
    36841 ttccgctttc tgaaattgtc tttgactttt tcgacaaatt gaaatcaagc acgaaaggct
    36901 atgcctcatt ggattatgaa atgtctggtt atcaaaaaag caagttagtg aagatggaca
    36961 tcttgctgaa tggagaaaaa gtcgatgcat taagctttat cgttcaccga gactttgcct
    37021 atgaacgtgg aaaagcaatc gttgaaaaac tgaaaaaact aatcccacgc caacaatttg
    37081 aggtgccaat ccaagcagct attggacaaa aaatcgttgc ccgttcagac atcaaagcct
    37141 tgcgtaaaaa cgtcttggct aaatgttatg gtggagatgt ttctcgtaaa cgtaaactct
    37201 tagagaaaca aaaagaaggg aagaagcgga tgaaacaaat cggctccgtc gaagttccgc
    37261 aagaagcctt catggccgtt ctgaaaatgg acgaggacga accgaagaaa tagcaagggt
    37321 ttgtttgaaa acagtgtaaa aaataaacca tgtttacatg gatttgccac cggtttgcca
    37381 ccggtg
//
LOCUS       DACCYH010000018        36851 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.018, whole genome shotgun sequence.
ACCESSION   DACCYH010000018 DACCYH010000000
VERSION     DACCYH010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.018"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(94..822)
                     /locus_tag="IVY85_000884"
     CDS             complement(94..822)
                     /locus_tag="IVY85_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP8043918.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            981..1862
                     /locus_tag="IVY85_000885"
     CDS             981..1862
                     /locus_tag="IVY85_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8043919.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(2099..3142)
                     /locus_tag="IVY85_000886"
     CDS             complement(2099..3142)
                     /locus_tag="IVY85_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8043920.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(3145..3813)
                     /locus_tag="IVY85_000887"
     CDS             complement(3145..3813)
                     /locus_tag="IVY85_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8043921.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            3955..6291
                     /locus_tag="IVY85_000888"
     CDS             3955..6291
                     /locus_tag="IVY85_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8043922.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(6406..6717)
                     /locus_tag="IVY85_000889"
     CDS             complement(6406..6717)
                     /locus_tag="IVY85_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043923.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            6898..8373
                     /locus_tag="IVY85_000890"
     CDS             6898..8373
                     /locus_tag="IVY85_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP8043924.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            8420..9004
                     /locus_tag="IVY85_000891"
     CDS             8420..9004
                     /locus_tag="IVY85_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP8043925.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            9006..9389
                     /locus_tag="IVY85_000892"
     CDS             9006..9389
                     /locus_tag="IVY85_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP8043926.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(9706..9990)
                     /locus_tag="IVY85_000893"
     CDS             complement(9706..9990)
                     /locus_tag="IVY85_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP8043927.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(10342..11052)
                     /locus_tag="IVY85_000894"
     CDS             complement(10342..11052)
                     /locus_tag="IVY85_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP8043928.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(11049..12521)
                     /locus_tag="IVY85_000895"
     CDS             complement(11049..12521)
                     /locus_tag="IVY85_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8043929.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            12659..13132
                     /locus_tag="IVY85_000896"
     CDS             12659..13132
                     /locus_tag="IVY85_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8043930.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(13285..13836)
                     /locus_tag="IVY85_000897"
     CDS             complement(13285..13836)
                     /locus_tag="IVY85_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8043931.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            13941..14471
                     /locus_tag="IVY85_000898"
     CDS             13941..14471
                     /locus_tag="IVY85_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP8043932.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(14954..15397)
                     /locus_tag="IVY85_000899"
     CDS             complement(14954..15397)
                     /locus_tag="IVY85_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8043933.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(15413..16573)
                     /locus_tag="IVY85_000900"
     CDS             complement(15413..16573)
                     /locus_tag="IVY85_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8043934.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            17080..18780
                     /locus_tag="IVY85_000901"
     CDS             17080..18780
                     /locus_tag="IVY85_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043935.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(18882..19715)
                     /locus_tag="IVY85_000902"
     CDS             complement(18882..19715)
                     /locus_tag="IVY85_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP8043936.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(20019..22184)
                     /locus_tag="IVY85_000903"
     CDS             complement(20019..22184)
                     /locus_tag="IVY85_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8043937.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            22444..24738
                     /locus_tag="IVY85_000904"
     CDS             22444..24738
                     /locus_tag="IVY85_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8043938.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            24731..25396
                     /gene="pgmB"
                     /locus_tag="IVY85_000905"
     CDS             24731..25396
                     /gene="pgmB"
                     /locus_tag="IVY85_000905"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8043939.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            25389..26396
                     /locus_tag="IVY85_000906"
     CDS             25389..26396
                     /locus_tag="IVY85_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8043940.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            26418..27437
                     /locus_tag="IVY85_000907"
     CDS             26418..27437
                     /locus_tag="IVY85_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8043941.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(27718..29085)
                     /gene="nhaC"
                     /locus_tag="IVY85_000908"
     CDS             complement(27718..29085)
                     /gene="nhaC"
                     /locus_tag="IVY85_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8043942.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(29194..30615)
                     /locus_tag="IVY85_000909"
     CDS             complement(29194..30615)
                     /locus_tag="IVY85_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8043943.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(30858..32735)
                     /gene="tdc"
                     /locus_tag="IVY85_000910"
     CDS             complement(30858..32735)
                     /gene="tdc"
                     /locus_tag="IVY85_000910"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8043944.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(33026..34282)
                     /locus_tag="IVY85_000911"
     CDS             complement(33026..34282)
                     /locus_tag="IVY85_000911"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP8043945.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            34862..36163
                     /locus_tag="IVY85_000912"
     CDS             34862..36163
                     /locus_tag="IVY85_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8043946.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(36232..36831)
                     /locus_tag="IVY85_000913"
     CDS             complement(36232..36831)
                     /locus_tag="IVY85_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8043947.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        11355 a         7560 c         6622 g        11314 t
ORIGIN      
        1 atagtttcaa aatcagtttg tcaaatacaa aaaaactgac taaaagattt gatccttttt
       61 agtcagcaaa aaatggcttc tctttgattt ttattataac agtggcataa tccaatttgt
      121 ccacatccag ctcagtggca tgataacgat cattgcaggt atcatgtcag ctactgggaa
      181 cattttcacg ttgatcattc taaaaccggt agcgaccatc aagaatccgc cgcaagcttt
      241 aaaatctaag atcatgtctg gtgtagtcaa tggataaata aattttgcca ataagaacaa
      301 aatataaaaa ataatgaatt ggggaattgc tatcacagaa acgacatacc ctaaattaca
      361 cgcaaaaata gctgctgtga aaaaatcaag gacagattta gaaatcaaaa tcgtactgtc
      421 tcctgtcatc ccagaatcta aacttccata gatccccgta ccacttgcac aaaatagaac
      481 aatgaccgtc actaatgtgc tgataaattc atcgtgggtc ataccaagct tttcactagg
      541 gaagatttta gaaataggta cttgcatcag cgcagccccc ttatttatcc cattacctaa
      601 atgaacaatc aaaccaatcg ctgttcctat cactaaagca aaaataacag ccggcatatt
      661 tttcatcggc gcaatggaat agattcccat ccccatcgaa cacacaccaa aaaccatcgt
      721 cagctgtgct ttaaaatgtt cacttaattt atttcctact aatccgccaa ttagtccgcc
      781 aaacaaaacg gaacctacat tgatcaaaac tccagttggc atcaaaaaac tcctctcttt
      841 ttctacgaag cgaaaaaaat cagaaaaatt tctgtaatta ttgctttcgc tgcaagaaag
      901 attgtaacag acagagaaga ttacaccaat tcattttttg tgttaaagta ttctttattg
      961 gaataagtag gaggaatatg atggatatcc ataaattcga agtttttctt gatttagccg
     1021 aaacaatgaa tttcaccaaa actgctgatc gacaattcac cacccaagga aatatttcta
     1081 aacagattct ttcgttagaa acagaattag acgtcaaatt attcgaacgc gcccatagaa
     1141 aaatcgaatt gaccgaagct ggaagcttat tgctcccata tgtaaaaaat gtcgtagaac
     1201 aataccatgc tatgcaagaa gtgcttgaaa cctatacgaa agacaacaat ctttcattaa
     1261 atatcttaac gataccgaca atgatcaatt ataaaggttt ttccaaaatc accgagtttt
     1321 tgaaacgcca tcctgaattt acagtccagt tgaaagaagt ggaaagtgtc gaattaactt
     1381 acaatgacgc caatgaacat agtgacacga tccactttgc tagaagtttc caggccccac
     1441 caagttcgat tgagtggttg cctactgaaa cagacgattt tgtagcagtt ctacctaaaa
     1501 atcatccttt agctagtaaa aagaaattgg atctaagcga actgaaaaag gagaactttt
     1561 tgctccttgg ccccactacg aacttgtatc aacctgtact agatctgtgt cgcgaagctg
     1621 gatttgaacc aaaaatctct tataaaggag caagaatcga cttgatcatc aatatgattg
     1681 caaatgacat ggggattgct attgttatgg aaaaaacagt caagaatcta ttaaaagaaa
     1741 atactgtgat tctccctatt tctccaacaa cagagagtta tcttgcattt actcgtaaag
     1801 tcggtgagca ttctttggct tcagatacat tttgggagta tttaaatcaa gaagaaaact
     1861 aagccctaca ttttctgtcc atctatccac ccaaaaaaag agagcgacgc atgactttcg
     1921 ctctctttct tgcatcatgt ccttgtttca atgacacctc taacatttct cgtagttcaa
     1981 taagaaagca gcgattttta tgccaaaaga accgtaagaa catttcccta atcacaaaaa
     2041 aacgactaaa aacccctaaa aaccttcaat agaattttta ggggttttat tctttttcct
     2101 attgtttact taaccgtatc attgctgtcg ttcctactcc ttcgatactg ctataatcaa
     2161 tacgtccatc atgcagttct agaatttttt taaccagtga aagtcctagc ccattcccag
     2221 aaatttgacg acttgtatct ccttgataaa atttttcaaa cattcgatct ctggtctctt
     2281 cattcattcc tacacctgaa tcggtcactt ccagtacaat ctctgtggct gtctcaaata
     2341 gtttgatatg aatctgtcca ttgatttgat tgtactttat ggcgttatcc atgatattca
     2401 gccacacttg gtacaaaaac tcttcattgc ctgtgtaatt tactgcagca agttcgatat
     2461 ccaattctag ctgttctttc tcccatttag gctgaaaaaa aacgattact tctctgattt
     2521 gttcatctaa tcgatactct ttcttttcca gttggatacg ctggttttcc agtttgttca
     2581 attttaagat gttctctgtt aattgtgaaa gctgcgtgat actttcgata atccgatata
     2641 aaaatatttg gcgttcttcg tcagagagat ttggggcttg taacaattga acatatcctt
     2701 ggattgttgc tagtggtgtt ttgaattcat gggatacact ggacacaaag tcattcctca
     2761 gtgtctcgat actattgagt tcttggacca taacgttgaa atctttatac agttgctgta
     2821 cttctgctac tttctgctgt tcatccattc gaatcgaaaa atctcccgtt gctaccagac
     2881 tcatatttgt ccctagcgca ctgattggat gcaggatacg ccgcccaaca aaaatcgata
     2941 ttcctgtacc cacgaccata ctgaaccccg caaagacgaa taacgggtat aacgttggtt
     3001 ttccagccga ttcagcaggc gagatagaat gtcgagacaa taagaaaata aaaccgagaa
     3061 aacacagcag tgtcactaag atcgaaatga agacaatact agcaaaatag atccataatt
     3121 gtgaaaatag cttttttttc atctttaaat aaccgcctta tagcctagtc cccgtactgt
     3181 gatgatttga aaatcttccg tcttttcaaa acgggtcctt aaacgtttga tatgtacatc
     3241 gatcgttcgt tcttctgtat tcgtatctaa tccccaaata tcatccatca actgttggcg
     3301 agtgaagatc ttgttgggat aagacagtaa tttatacaga agcagaaatt ctttttgagg
     3361 cagaatcaac gaatgctgat cttgtaacac tgtataagca tctagctcta attttgtttg
     3421 tccgattatc aagctactgc tgtaattgat ctttgaacgt cgaagcaatg cttccacacg
     3481 taggatcatc tcattgacat cgatgggttt tgtcatataa tcatctgctc ctaatttgaa
     3541 cccgcgtttc ttatcctcaa aatcggcctt tgccgtaatc attagaacag gtgtgtcaaa
     3601 tctggtgcta cgtaatgagt ctagcaagtc atagccatcc attttcggca tcatgatatc
     3661 tgtaatgatt aattctatac gtgtcgcttc tagttttttc agcgcatcct ctccattcaa
     3721 tgcggtgatg actgaaaagc ccgctcgttc caacacagaa cggtaaagca actgtaattg
     3781 cggatcatct tctactacta aaatacgatt cattgatctc cctctgtttt ttgaaaaatt
     3841 tctagatttt ttcttgtagt tcatcttcag ttaacattta tttcgtatac tacgatcatt
     3901 gttctgatat acttattagt ataccgaata tttcaatacg ggagaagggt gattatgctt
     3961 caattaaaag atataaaaaa atattataaa gtcggcgaaa ccaccaccaa agcattggat
     4021 ggtgtgtctg tcgctttcag acaaaaagaa ttcgtagcga ttttaggtcc tagtggttct
     4081 ggtaaaacga ctatgctgaa tgtgattggt ggactagata attatgattc cggtgatatg
     4141 gtaatcaacg gaaagtctac taaagacttt aaggacagtg actgggatgc ctaccgaaac
     4201 aactctatcg gtttcatttt tcaaagttac aacttgattg gtcatctagg cattattgaa
     4261 aacgttgaac ttgggatgac attaagtggt gtttctaaag acgaaaaacg aaaacgggca
     4321 gaagatgcat tgcaccgagt cggcttgaca aatcatatgc ataaaaagcc aaatcagtta
     4381 tctggtggac aaatgcagcg agtggccatc gctcgagcac tagccaatga cccagatatc
     4441 cttttgtgcg atgaaccaac cggagcgtta gatacagaaa caagtatcca gatcatggaa
     4501 ttgatccaag agttatcgaa agaaaaatta gtgatcatgg tcacacataa tcctgaactg
     4561 gcccatcaat acgcagatcg aatcattgaa ttttctgatg gtaagatctt aacagattcc
     4621 catcctcaca tcgaacgacc aaaagatgat caatttaact tacgcagaac aaagatgagt
     4681 ttttggacag cgttaaagct ttccttcaac aatatccgaa caaaaaaagg acgtactttc
     4741 ttgacctcct ttgcttccag tattgggatc atcggaatcg cgatcgttct gtccctttct
     4801 tctggattcc aaaaacaaat cgataacacg caagctgaaa cgatggcgaa atttccgatt
     4861 actatttcta aggtaacgac taaccagact cgtgacgatg ccggattggg tgcaagcaaa
     4921 gcggattatc cagacagcaa aacgattact gcaaaagtca gcgaagaaga taaagcacag
     4981 cacacgaata agattgatca aacatacgtc gattacgtaa cagacatcga tcctaactta
     5041 agtaataata tcggatttac ccgtactaca ggaatcaatc ttttacgtga tgtaaatggc
     5101 aaagtacagc ctgtcagctt ctcgaatcaa aatcctgata cggaatctct ttctttatct
     5161 agtacgatgt cagcaatgac aggtgtggga gtgtcaagtt tccctacaca gcttgacact
     5221 agcaaagaaa actttttaaa agacaattat tctttacttg ccggaagtta tccagcctca
     5281 gcaacagatg tcgtgctgat cgtagacggc aataataata ctaatatcaa cgcattgaag
     5341 aatcttggat ttgatgtgaa agaagacgaa aaacttgatt ttgatgatat cgttggtaca
     5401 acattcaaat tagtaaacaa caatacttac tatacaaaac ttccaactgg gaattttatc
     5461 cctaatacag attatgatgc gatgtatcag aatgcttctg atgaattgaa gatctcgggg
     5521 atcctacgcg tgaaatcttc ttctacaatg aatctcttat ctccaggaat cgcttatagc
     5581 gatcaattaa cgactcaaat cgtcaacgaa aacaaagaat cagaaatcgt caaagctcaa
     5641 agagacagtg gtgtaaatgt cttaactact gaaaaagttg acgaaaatgc caaacaaaca
     5701 ttgcttagtt atttaggtgg agacagcttg ccttcaagta tcatgattta tccgaacaat
     5761 tttgaagaca aagaaaagat cttggattat ctagatgatt acaataaagg caaatcagat
     5821 gaagataaaa tcatttatac tgatttagct ggaacgatga ctgaactgac tggcggattg
     5881 atggatgcta tcacttatgt ccttattgct tttgcaggga tctctttagt aacaagtatg
     5941 atcatgatca gtatcattac ttatacatct gtgattgaac ggacaaaaga aatcggtgta
     6001 ctaaaagctt taggtgcacg taaaaaagac atcactcgcg tatttgacgc agaaacgtgt
     6061 attttaggta tttcttctgg gatattaggt gtgtttattg catggctagc tacgttccct
     6121 atcaatagca tcttatacaa catgacggat ctaaaaaatg ttgctcaact aaatcctgtc
     6181 catgcgatta ttctagtgat cgtctctact atattgacga tgcttggcgg tcatcttcct
     6241 gcacgaatgg cagcgaaaaa agacgccgct atcgctttgc gtgcagaata aggttgtgac
     6301 aaccacattt agatttgagt cataagagcg ctgaaatagt agaaaacagc aagaaacgag
     6361 gaagtaatat gttccttgtt tcgcgctgtt ttttttaatt attttttata attctggtat
     6421 acttttcttt cctttgtacc acaactcttc tggcgtatac ctgtcttttc catttatgaa
     6481 tactgttcca tctgcttcaa tcgttacttc agtcccaatc cacatattac gagaaagtgc
     6541 gagaagattt tttctttttc ctcttccttt ttttccaaat tgcaaggcat cggttatctc
     6601 ttctgtccaa tgagatttca cgtattttac ttctccgttt tcaaatgtaa ctttcaaaat
     6661 attcgctata ttttccactt ttactgcttt aggaaatgac tcctccattt ttttcacttt
     6721 ccttcctcca acactaagta tgcgctttct tttagtgata agtatatcat agtttattct
     6781 attatcaaaa aaacgcatcc attccattgt agaatacttt accgaacaag ttgaattgtt
     6841 ttcctctttt tcacctttta ctatagatag gtaaagacaa ctaaggagga atctctcatg
     6901 acagatcaac cgtatgattt aagaaaagtc atcgaagaac taaaaacact tccaggtcaa
     6961 tatcacgaaa cagatattga aatcgatcct gaagcagaat tatctggtgt ttaccgttat
     7021 atcggtgctg gcggtacagt aaaacgtccg actcaagaag gaccagctat gactttcaac
     7081 aatatcaaag gtttccccgg cacacgtgtg aacatcggaa caatggctag tcgtaaacgt
     7141 gtaggtgaaa ttttgcacca tgattatcga acacttggtc gtttcttgaa agacgctgta
     7201 gaaaatccaa tcaaacctgt gaaagtagac aaagcagacg ctccagcaca agaagtcgtt
     7261 catctagcta ccgatgcaga cttcgatatt cgcaacattt tgcctgcacc tacaaatact
     7321 cctgaagatg ctggtccata catcacaatg ggtgtagtct taggatcaga tccagaaaaa
     7381 acaatgagcg atgtaacgat ccatcgtatg gttctagaag ataaagatac gatcggtatg
     7441 tacatcatgc ctggcggacg ccacattggt cacttccaaa aacaattcga agccttgaat
     7501 aaaccaatgc ctatcactat caatattggt ttagatccag caattgccat tggcacgact
     7561 ttcgaaccac ctaccactcc acttggctac aatgaactat ggtgtgcagg agctttgcgt
     7621 aacgaaccta ttcaaatcgt tgatggtata gcagtcgatg aagtgggtat tgctcgtgct
     7681 gaattcatca ttgaagcaga aatcatgcca aatgaaacaa tgcaagaaga tatcaacacg
     7741 aactcaggaa aagcaatgcc agaatttcct ggctatactg gggatgctaa tccagctgta
     7801 caagtagtga aagtcaaagc agttactcac cgaaaagaca atccaatcat gcaaacaacg
     7861 attggtccca gtgaagaaca cgtatctatg gccggtattc ctacagaagc aagtattctt
     7921 gccttggtcg ataaagctat tccaggaaaa gtagtaaatg tctacaatcc tcctgcaggt
     7981 ggtggtaaat tgatgaccat catgcaaatc cgtaaagaaa acgaagcgga tgaaggtatt
     8041 cagcgtcaag cagctattct tgctttatca gccttcaaag aattgaagac agtcatttta
     8101 gtcgatgaag atgttgatat tttcgacatg aacgatgtag tttggacttt caacactcgt
     8161 ttccaaggag ataaagatat catcgtgatc ccaggaatga ggaaccatcc attggaccca
     8221 tcagaattac cagaatataa tcctggctct attcgtttcc gtgggataag tgcaaaaact
     8281 atctttgacg gtacagttcc tttcgatatg aaagaacatt ttgtacgtgc aaaattccaa
     8341 gaagtagaag attgggaaaa atatttgaaa taatgaatga tctgcaactg ttttgacatc
     8401 ataaaaagga gtgcggaaaa tggaacaatt ggcacaacgg aagaaaaaaa tcgtgattgg
     8461 tgtaagtggc gcttcaggca ccatttatgc catcaaccta gtgaagaaac taaaagaata
     8521 tccgatgata gaaacacacg gaatcatcag tccatgggca aaacagaact taaagctgga
     8581 atccgactta tcactaactg aattcgaaag cctgtttgat taccattatt caaacaaaga
     8641 tttaggtgct gctgtcgcca gcggatcatt tttgaccgat ggaatgatca tcgtaccggc
     8701 aagtatgaaa acagtcgcag gtatctctgt tggtttaggc gacaacctga ttgcacgtgc
     8761 tgctgatgta acaatgaagg agcaacgaaa gttagtcatt gttcctcgtg aaacgccatt
     8821 aaatgccatt cacttggaaa acttgactaa attagcacga ctaggtgtgc aaatcatacc
     8881 accaattcct gctttttata atcagcctca aacgatccaa gatcttgtta atcaccatac
     8941 aatgaaactt ttggatcaat ttgaaattgt aaatgagcat tcaaaacgtt ggcagggtga
     9001 ttaatatgga aaaagaaacg atagttgaag taaccaaact aggtttcacg atccaagctg
     9061 ctgttaaagt aatcggtaaa gacttattga tcactttaac aggcggagat acaccacata
     9121 tcggaacagt cactacattt tctcgtgata cagagatgca aactgtacgt ttccctagcc
     9181 acgatggtcg gttccataaa gacgatgtct tagccgaaaa aatagctgtg atcatcaaaa
     9241 ctgccgttcc gggaagttgt acgatcactt ccggtatcca tgttgaccat atctccaatg
     9301 cacaaatcaa cgcctctttt ccgatgtcag aagagttagg ttacaagatt ttagattggt
     9361 tggaaaaaat agatttccat atcaaataac cagtttatta taaagatggt gaaaaaccat
     9421 tatgaaagaa gaggttgtga aatcagcgtt atgacctgag tatgagagaa aaaaatggaa
     9481 aaatagcttc tcatattttg ccgtttttga gctaataccg agggacagct gattgaacgc
     9541 cgtttatcaa gaggttgtga taaagccgct tcgtttcgag tagtaagaaa aattttgtaa
     9601 acatgggttt ccacattttt gcataaattt gacttactac cgagaaactg gcttttgaac
     9661 accgtttatc aagaggttgt gacacgtctt ttgtcacaac ctcttttatt tttgcttcac
     9721 tgtaactgct acaatatcac cgatttcttt tccgatgttt ttacgaatat cttttcgtag
     9781 tccaaggatg tagcagactg aaccatctgg atttttcacg cccatattca cgatactgcc
     9841 ttcatacggc acattgtcaa aagttgcttg gactttaact cttccagcac cgaattcttt
     9901 tcttatatca tacggaaaaa taatatatgc cccgcccttg ccgacttcgg aagcatagat
     9961 ttttgattca aaagaataaa tttccctcat tttatttctc ctatttttta catctagtat
    10021 agcatagcaa aaaaaagaag gaactgtgac aaaagtcttg tcgcagttcc ttttttcgaa
    10081 taaacggtgt tctcctgcca actcctcggt cacaagtcac aatagtcaac aaacatgaga
    10141 agctgttttt tgaaattgct ccttgtgact cagagttaaa cggcaggctc acaacctctt
    10201 ccttaaacgg tgttcaaaag cttgcatcta cggcactaag cccaatcaga tgaaaaatat
    10261 ggagtgctat ttttatctga ttgttcttac tgcttgatgc agagcagctt tttcacaacc
    10321 tcctacatgg tgttcaaaat attattcgcc ttttgcttct ttcactaatt ttgcgaatgc
    10381 ttgttgttgg aattcagcag ctaaggcttt cgtacgttgt tcttgctctg ctttgctcat
    10441 ttttggattt tcagtcattg cttttaccat accgtcttga acataagctt ttgcttcact
    10501 ttccatcgta ctttgctgtt tcttcactaa ttcagctaac ttggctgctt ggtccgtact
    10561 taattctacc catgtctctg ggattttgaa tgtattcact cggctgtcat attcgtgatt
    10621 gttatcagca atattgaacc agaatttata catgtcgttt ttgtattccc aataagtagt
    10681 tttcgtcact ttttcagcat cgccgtctgt cttttctact ttatttgttt cgttatccgt
    10741 accagttgct tttggttttt cttgtttttc atttgttcgg taaagataca ctttttcttt
    10801 tccattccct aaagctttat acaaaagcat atctgctcct tgggaatcag cactggaaac
    10861 aattttttct gtttttgttt ccgttacttt ttccattcca aagtgattgg acaaattagc
    10921 gacgatcaaa cttagtgaag cgacgaagat tagtccgaaa atcaaagata aaaaggtttg
    10981 ccatgttttt ttagcgaaca cgttcgttaa agcaaaagca agcaagctga caatcaaaac
    11041 tccaataatc atgcattatc ccccgctttc attagaacag gttctttttt cttcaataaa
    11101 aaggcaataa taaagccaag aatcccaaag atgatcgcta cgataaacgc ggcatggtaa
    11161 cctgacagtg tggcattgat tgcctgatct ttataagcta atggcgtatt atccaataca
    11221 gatttgcttg ggatatttgc ttttgtcaca ttcgtcaaca cactgatcaa aatagctgta
    11281 ccaatcgagc ttgcgatttg acggaatgta ttatttaccg ctgtcccatg actgatcaag
    11341 ttatttggca atgcattcat accagaagtc gttaccggca tcatgaccat ggagatccca
    11401 aacatacgga tcgcataaag gatcacgata tataaaactg gagtcgtttt tgtcaagaaa
    11461 gcaaatggtg cagttgccgc tgtcaaaatg aacatccctg tgatggctaa acgtttcgcg
    11521 ccgtatttat cgaaaatcgc acccgtcaca ggcatcatag cccccatgat caatgcaccc
    11581 ggcaacaaca tcaagccaga atggaatgct gattctccac gaatattttg aatatacaat
    11641 ggtacgacca tttctgcccc taccatcgcc atatttgtta caccagctaa aacagctgca
    11701 atcgtgaatg tagatgattt gaatacacga agttccaaga atggatgttc caaatgtaat
    11761 tgacgccatg agaacaaggc gatgaaaatc acaccaacaa ctaagaaacc aattactttc
    11821 aagcttcccc agccgtcatc tcctacacta gaaaatccgt aaagtaaact accgaatcca
    11881 atcgttgata aaatagcgga taaaatatca atgctaggat ttgacaatgg aataacactt
    11941 ttcattagga agaatgctaa aaccaagaca agaacaacga ttggcagtac cataccaaac
    12001 aagtcacgcc aatggtagtt atcaatgata taaccagata atgtagggcc taaagcagga
    12061 gcaagtccaa taacgatccc tgacatcccc attgctgatc ctcttttttc tggcgggaaa
    12121 atagatagca tgatattttg caataatggc atcgatactc ccacaccgca agcttggatc
    12181 aaacgaccga ttagtaatgt actaaaatta ggtgctgtaa agcaagtaat cgttccgatc
    12241 aaaaagatcg tcatggcaga aagatataaa tttcttgaac tgaatttatt gatcagccat
    12301 gctgttattg ggatcatgat cccgttgact agcaagaaac cagtcgttaa ccattgcaca
    12361 tcagacgctg aaatatcgaa agctttcatc aatgtaggaa aagctgtcgt caataaggtc
    12421 tgattcaaaa cggtacaaaa tgtaccaatt aataagacaa caactaaaag tgtccgatta
    12481 tatggttttc cgtaaatatc tacgggttgg ttgttactca tatttctcct tctttctgaa
    12541 ttttttctga aactactaga atactttagt cctaaattcg ctatttgtca acctacttga
    12601 cacataagtt aatacggata taattaaggg aagcaagagg acaaaaggag tgacaaaaat
    12661 ggttggggct tcccttaaac gtttgttgga aaatgatcaa ttggttgttg gcatcagtga
    12721 attaagtgat atcgtaggtg tgtctccacg tcaattaagg tattgggaac aaaagggatt
    12781 cattcgttcg atcgaagctg atgcgaattg tcctagaaaa tatcgtttac ctacagtcat
    12841 aaaagtagaa ctgatcaaaa aatatttaga tgacggattt actttgtcaa aagctgcaga
    12901 aaaggcagaa atccgccaaa agaaaatgca ccatgtccgt aaagtttttt caaaagcaat
    12961 caaagatatc gaattgatcg acgaacgcta tacgatttta caaatcggta acttcaaaga
    13021 agagaatgct aagatgtaca tcattcatga caacgagaca gacgaattaa cttacaagct
    13081 ccttcctgtc gatgaaaaag ctgactacga acagttgtta aaagaactct aaacgccact
    13141 aatctcctaa gtaccaagct ttttaataag tagatgctac ataaatactt gaaaaaaggc
    13201 ctgtttgaac ataagaatat ccgaacggtg atctgcgttt ctttcatata gaaaggaatt
    13261 ctccatcact gttcggatat ttttttattt ttttcggtta ctttcttcta atacatctgt
    13321 tagccaattt gcatattctg tttcccgatt gatcgcatgc gctaaaatca ggtagtgtcc
    13381 gtaattttca tgtttttctt tttcagtagg aaagatcgtt ttttttcgct ctaatagatg
    13441 aagtcgtttt tcttcatgaa ggtcacgctg ctgctgaata atttctacta atgccggatc
    13501 attcacgtct ttgacgaaat acaatttcag aacaaattcg tctcgattaa ccggtagttc
    13561 attggtgggt gtatgaatcc actctgccaa ttgttccttc ccttcttctg ttaattcata
    13621 aactttcttt tccaattttt cacctgtgat ctctacttga tgctgaatga ttccttgctc
    13681 ttccattttt gcaagttcag gataaatctg actatgtttt gcttgccaaa attctcctat
    13741 ttcatttttg aacgattgat tcaattcata tcctgtcatc ttcttcttac ttaataaccc
    13801 taacaaaata tatcgaagtg tattttttct tgccatcagt tttccctcct tgacaaatct
    13861 ttaaatccat tctatcataa atgagtaatc gaaaacatgt agtttattac atgttttaat
    13921 ttataaggag atgcttaaac atgaaagaat ttaaagaatt ggaagatttt attggaactc
    13981 actttatcta tacgtatgac aatggttggg aatatgaatg gtatgccaaa aatgaccata
    14041 ctgttgatta ccgtatccat ggcggaatgg tcaaaggccg ttgggtaaaa gaccaagaag
    14101 ctgatatcgt taaattgacg gacggcgtat tcaaaatcac ttggacagag ccaacaggga
    14161 cagacgtggc attggacttt atgccaaatg aaaagaaact tcacggaatg atctttttcc
    14221 ctaaatgggt tgaagaacat ccagaaatca ctgttacttt ccaaaacgaa catatcgcag
    14281 aaatggaagc agctcgtgaa aaatatgaaa cttatccaaa attacttgtt cctgaatttg
    14341 ctacgatcac ttatatgggt gatgcaggta tagacaatga agatgttatt agcgaagaac
    14401 catacgatgg aatgccagat gatatccgta gcggaaaata cttcgatgaa aattataaac
    14461 gagtaaaata aggttgtgaa agagaggttg tgaaatcagc gttctgacct gagtattaga
    14521 gaaagaaacg gaaaaatagt tcctcatgtt ttgccgtttt ttgagctaat accgagggtc
    14581 agctgattga acaccgttta tcaagaggtt gtgaaatcag cgttatgctt tgagtaataa
    14641 gaacgctagt tagaaaatag cttttcatat tttctaggct agtgggctta ttaccgagaa
    14701 gcagccgttt gaacaccgtt tatcaagagg ttgtgaaatc agcgttttga cctgagtatt
    14761 agagaaacaa atggaaaaat agttcctcat attttgccgt tttttgagct aataccgagg
    14821 gtcagctgat tgaacaccgt ttatcaaaga ttgtaacaac tgcatattca cacagttaaa
    14881 ttagtgactt caaaatgaaa taatgctccc agcaaaaaat taggctggga gcattatttc
    14941 gttattctgt tcatcaaaaa tcagcgtttt gattccattt catcacaagt tcctcctcat
    15001 ttactgcttc atctttttgc tgtgtttgaa ttgtaaaacc ttgtgaaaga tagaaatgaa
    15061 tagctgattg atttttttga tagactgtca aagtcagtgt tttattggtt tcttttattt
    15121 ttttcattag ttgcgtaccg attctttgtt gccggtattc ccttttcaca aagattcctg
    15181 cgataaactg atcaatcacc ccagcaaaac caataatttc ttgatctatt tctgctaagt
    15241 aaatcactgc atctggtaaa gctttcctaa cagattctac ttgattctcc caataagttg
    15301 atggaatgaa ttcatgagaa tccaagttag catgcagcca aatcgataga acagattcca
    15361 gttctttggc agacaatttt gttgttgtgc ggatcatttt ttctctcctt acttattcat
    15421 tgattttaaa ataaaaacca tttttttgaa gctcttttac aagttttcgc ccctcaaaaa
    15481 agtatatctt ctcttgtttt acttttctaa taccaaaaga aacagcaact ttaggatcat
    15541 cagctgaaac gattcgataa gttccaccag gcaattgatc cagcttgact ttcccatgat
    15601 ggtcgctttg tactgaaaac ggcacaccat ttctagtaag tgtttttttc ttatgtttca
    15661 aaatgaatga actaccattt accggtttcc ctgacgaatc actaataata aatgaaaaat
    15721 caagcacttt tttacttaat ctatactgat agattagtcg tccgataaac actaacagta
    15781 tgactacagc tattatcgtt cctgcaagaa taaaccattg ccgcatggtt ctttcctgat
    15841 ttccttttcc ttcttctttt tttaccgatt cgttataagg aatccgatgt cctgtcacta
    15901 gcaaacgatg cgtgttgatc atatacggcg tacatgtaag caaggtaact aagtcttctc
    15961 cttctgaaat cgttagaaca gacgtgtcat ctggcagtac cacttgtata tttttaactt
    16021 gataagccaa tttctccccc aaaacagtca aaataaacgt gtctccgttt tctaaacgat
    16081 ctaattcggt aaataaccgt cgattaggta aaccgctgtg agcagagatc actgaatgcg
    16141 tcgaatttcc accaagcggg aaagaggttc cttgcaagac gcctgctcca ttttcaagga
    16201 tcctaccatt gagccgatca taaagtgaaa tatttaattg gatcttaggt atattgatac
    16261 ttccgatcag ccattcctct aattggctgg atgttgtact ttcatttctg ttcttttcat
    16321 tgaaaggatc agtaccagga ttgaatcctt ggtcagttaa tcgttggttt ttctcctcca
    16381 ttttctttcg ctgcacttca atttcagctg cggtccgttt ttgtacttgt tcgatacgtt
    16441 tttgatcaat aaaactattt aaagaatcca cataaaatgg atataaagcg atcagtaacc
    16501 ctacgacgat acatattaat aaaaacaaac gaaacaaaag tcgacgagac tgtttttgct
    16561 tactctgctt cacatgtgtc ctcccttgca agtttttttg ttctttcttt tattatatgg
    16621 gttcaggaaa aaaatagaaa atgaaagccg atgaggttgt gaaaaagctg tcctgcatca
    16681 agtaataagt acagcccaaa aaaatagcta ctcatatttt ttgttgggtt gggcttatta
    16741 ccgcagatgc aagcttttga acaccgttta aggaagaggt tgtgaaaact gcgttatgct
    16801 ttgagtaata agagcgctag tcagaaaata gcttctcata ttttctaggc tagtgagctt
    16861 attaccgaga agcagctgat tgaacaccgt ttaccgagag gtcatgaaaa agctactttg
    16921 tttcgagtat taagaaaaat tctgtttcaa aacaaggaga tgcaacaaaa cttatgtcgc
    16981 atcctctctc cttagaactt tcacttatgt ttttcttgta aaattcataa atattttcat
    17041 gaattttcta atttcttgcc tacctctcgc ataggatata tgatgaattt atctaaagtt
    17101 tgcaaaggag ttcgacaatt gaaaaaatat ggcccatttt tagcaatttt tttcattgct
    17161 ttggcatttc tcatgcctca tttattcaca cataaactaa ttttgggtgc tgatgcccca
    17221 ttccattaca atcggttcta tgatgccgcg caacaaatta aatatgggga tttccattat
    17281 tttccttccc tttatggatt ccagcaaagt ggcaggatcg tgaatgccgt ttacggccca
    17341 tttttcgctt atttacaagg attgatcgtg ttgcttgcag gtagctggtt tcattatcag
    17401 attctttcaa atttcattct ttatcttatt tcaggatttt ctatgctgat tttactgaga
    17461 tatgtcaatg ttcgaaattg ggtaagtgta ccagtagccg ttttattcat gagtacgtat
    17521 tctattcaat actggtctat caaccaagga ttcactagct gggggacttg tttttatcct
    17581 ttatgtatga tccctattgc tgatttcgtc cttcataaaa agttccctgt tatcaaagtc
    17641 gctgtctcag tgggacttat gacacagatc catttactgt cttcgatcat gttagctttg
    17701 atgtatatcc ctttttatct ttattacttt tttaaccaga ctgaaaaagg aaaagcactg
    17761 cttcaactga ttaagtcggt cttgctttat tttgttctga cagcaaatat ttgggcagga
    17821 ttagctattg tttacagtgg gaataagatt cttcctccct ttgttaacag agaaatgagt
    17881 caaaaggcga tagatctgga tggcagttat tggctccatt accctaaggc ctttcctata
    17941 ttgatattat caggtttttt gctttttatt ttctttcaac aaaagaacac cttattccaa
    18001 aaaatcgtgt tgtacacaag tcttgttttc ttttttcttt ctactagctt gattccatgg
    18061 acgacattga tcgaaaaaaa tgtgcctttt attagtttga tccagtttcc atttcgattt
    18121 ttcgtgccat ttacgctatt gcttctactt taccttgccc tttcactgaa ccagtggtct
    18181 gaacaaaagt ggttccgtct tttaagtata tttggcattc ttattttttc agcacagacg
    18241 atccagaact tataccagca tctggaaaag tgggaaaatg aaacatttgt cagtcgacat
    18301 acttatctat tcgatacgcc ggaagaagca aggaaaacat tcttcagcaa agatatgtct
    18361 accagcttgt tcgttttcca aaaaaccaca cctgactatc tgccaatcta taaggagaca
    18421 aaagacaata aatacgaccg gtataacgaa gaaatactag aaaacgaaac aacttttata
    18481 aaaacacatc caggcggtct tttgacaatc aagtggaaaa acactgggga caaaactgtt
    18541 catatccctg ttatcgtcta cgatcggact gtgctacagc aaaatggaaa aacattgact
    18601 gattatgaag taacagatat cgggacccca atcgtgaagc agcaaaaagg aatcaacgag
    18661 ctcacgcttc attatcaaac gccaatctat ttctatttca ttctttctct tactttgatt
    18721 ggctggttta ccttgctatg tttatttatt tatcatagat acaagctttt acgtgcttag
    18781 gcaaaaggga taaatacatc cgatcaaaaa actctccaaa cggtataatc gtttggagag
    18841 ttttatttta atcagttttt ttatgttata tgttatattt tttattcgtt cacatctaat
    18901 tcgatttcca ccccgtagtg atcactgatg attggactat cgatcccatc aaaaatccgt
    18961 tgatattttt taacctgcat tccttcaggg acgaaaataa aatctatacg caatttttct
    19021 gtattttgtt cccaaccatc tattttcttt tccacagtag ctgcttttcc tttttcattc
    19081 gcgacaacat acgaatcttg aagaagaagg caactttctc ttacagtata ataaccttga
    19141 gagtctactg gattattgaa atcacctagg aaaaaaagtg gtacgcgagt ttccaaaaag
    19201 tagttttcca agtttttcca ctcaaaataa aagccacttt ttttatctgt ccaccaagaa
    19261 aagtggcagc tcgagacaaa aatgtcctga tcatctatct ttgttttccc aatcaagatc
    19321 ttgcgtgtct gataatcttc tttcttttga aattgggaga cggtaaggac ctgactttcc
    19381 aatgtttttt tcgtaagcag tgcatttcct tcttcatatt tatcataacc gatgtggctc
    19441 atttcccaac tccaaaaata ctcttttccg tgttccttta aaagctgaac taaacggtag
    19501 gcaaaattat ctttacggat aggtacttga ttctttaccg gacagaactt cattagcttc
    19561 gcaggttcta cttcttcaga ttccagtaat tggttgattt cttgtaaagc aatcacttca
    19621 taatcttcta acaggatctt atctgctaat tcttgcaatt ttttctctgg ttctggctca
    19681 agccagctat gcgtgttgag tgtcatgaat ttcattggtt ttctccttgt cttcatttcg
    19741 aaacaaaagg gttgtgacaa aatttttgtc acaacctctc cagcaaatag tgttctcctg
    19801 ctaactcctc agtaataagc tcaatggacc aggagctatt ttttgtttgg ctgtttttat
    19861 cacttggtgc agggcagctt tttcacgacc tctccactaa acagtattca aactgctgct
    19921 tctcggtaat aagcccaacc aaacgaaaaa taggaggagc tatttttgtt tggctgttct
    19981 tatgacttga tgcaggacag ctttttcaca acctctcctt acattttaat ggagccgatc
    20041 actttaccag ctgtcactgt acctaattgc ttgatcacta cttcttctac tttatcgttt
    20101 gtaaatacga tcatgatcgt tggatctttt tcttcgtttt ggatcacatc aagatccatc
    20161 tcagctaatt tcgttcctgt tcctacttct tggccttctt tgacagcaat ctcaaaagct
    20221 ggctgattca tttggactgt atcgattccc atatggatca aaacttcgat accatctttc
    20281 gtttccagcc caattgcatg tttgctaggg aaaatacttg tcacttttcc agcgatcggc
    20341 gcaacaactt ctcttgatgt tggaataaca gcaaagccgt cacccatcat tttttgtgag
    20401 aataccggat cttttacttc agataaagga atcacttctc cagaagctgc ggaaactagt
    20461 tcttgttcta tacctaagta ttgcttacta cctgcttgtc catttcctgt agaaaccatt
    20521 tctggctctg gaatctctgc tcctgattgc agcagatctt cgatatctga tttcaagaca
    20581 tctgcttttg gtccataaac tgcttggact ccattgtctt tgacgatcaa tcccatcgcc
    20641 cctgctcgtt tccaggcttc ttcagtccct actttttctc gatcatttac acttacgcgt
    20701 aaccgagtca tacaagcatc gacatctttg atattttctt ttcctcccag caaatgaatg
    20761 atctggacca cttgctgatc tgctgttcct gttccgctgt tagatacaac ggtttcttca
    20821 ctgtcattgt cgtaatttcc gttacgtccc ggtgtagcaa agttgaattt tttgatcaag
    20881 aaatttgcta agaaatacgt aacaacacca aaagcgatca cacacaacac gaagttgata
    20941 agatctccac ctagacccgc tttgatagct aacggtgtcc gagtcagcaa ttcgatattc
    21001 ccaaatgaat ggacccgcaa tggcaagata tctgccatcg caaatgcagc cccttggatc
    21061 actgaataaa tgacatataa tggcacagca gcaaacataa acatgaactc caatggttct
    21121 gttacacctg ttagaaagac agccaacgct gctgaaaaat acattgattt atatcttgct
    21181 ttcttatcag gatcgacatt acgatacatc gcaaaagcca ttcccatcaa gatacctgaa
    21241 gaaccaatca tttgtcctac tttgaaacga gccggcgtcc agttatgcaa cacaaactca
    21301 tattgcgaca tatcccctgc acctttaaga ttcactaaat cagtggccca agccaaccat
    21361 aacggatctt gcccaaatac ttgtgtcccc gcctgtgcgc ctgataaaat ctcatacgat
    21421 ccgccaagct gtgtatagtt gataggaatc gtcagcatat gatgcaatcc aaatggcaat
    21481 agtaaacgtt ctaatgttcc aaataaaaac ggtgctagaa tcggtgcgct ttcttgtgat
    21541 tctgcaatcc ataaaccaaa attattgatt ccagcctgta cgtttggcca caccacagca
    21601 agaatcaatg ccacgattgt tgaccataag ataacaacaa acggaacaaa gcgctttcca
    21661 ttgaaaaatg acaaagcatc cggcaatttt ctataattat agtacttatt ataagccatt
    21721 gccccgacaa atccagcgat gatccctacg aatactccca tattcaaagc tggtgcttcc
    21781 aatacacttg taaaaaatcc tttaaccatg atcttcgtac cgaacagagt gtgggtaaat
    21841 gcatcttcat tcgcaagcat gtcacttgtg acaccaaaga tagccccagt gatccgattg
    21901 attaaaataa atgagatccc agctgcaaat gctccgcctg ctctttcttt tgcccaactt
    21961 cctcctattg ctaaagcaaa taaaatatga agattaccaa taattgccca accaatattt
    22021 tcaattactc cgcccgttgt gacaagcggc gtccaatcag gattgatcaa gggaattgat
    22081 ttccctatac tgatcatcaa cccagctgcc ggcataactg ctaccacaac cattaaagcc
    22141 tttccaaatt tttgccaaaa ctcaaaacta aatatctttt tcattccgtt tagctccttt
    22201 acgcaatcgg tttcgttaaa cgtattatag cgtgcgtttt cttttttgta aatgctttat
    22261 tttttatttt tctgtctata ccattctata aaacctataa tacgtgtgtt cccttctata
    22321 attttctcct aaaaatttat ttttttaagc ttttttctat gaaatatttt cttttgtcta
    22381 ttgacttgtg caatcggttg cgtttattat gaacgtataa aacagctcat taggaggaga
    22441 aaaatgaaac agatcaaacg actatttgct attgatccat ggaaaatcag cacgacacaa
    22501 ttagataaaa aaaatcttcg actgcaagaa tctttaacaa gtattggaaa cggttatatg
    22561 gggatgcgcg ggaactttga agaaacttac tcaggggacc accatcaagg tacttatttg
    22621 gcgggtgtct ggtatcccga taagacacgt gtcggctggt ggaaaaatgg ttatccagaa
    22681 tactttggaa aagtgatcaa cgccttgaac tttatcgcga tagatatttc gatcaacgat
    22741 cagctgatcg acttggcttc tttggaacca gaagactttt actgggaatt agatatgaaa
    22801 aatggtgtat tatctcgtac attcacaatc actacttcta ctaataaagt acgcatttcc
    22861 ttcgaacgtt ttctgagcat tgtcaaaaaa gaatcagctt atatctattt aactatagaa
    22921 atgcttgaag gtagcggaac agtcaaactc gtatcaaaat tggatggaaa tgttcaaaac
    22981 gaagacagca actatgatga acatttttgg aatgagattg atcgtggaac tatagaagaa
    23041 atcggctatt tgacgactaa aactattcca aacaactttg ggattgaaca atttactgtt
    23101 actgcagcaa tgcgccattt cgttaatgga aaatcagtca tccctgacta tcacgaagat
    23161 actttagcat tacatggttg cttgtctgca gaattatctc aaggtgagac atggtcagtg
    23221 gaaaaagaaa ttatcgttct tactagtaga gatattccag aagcgcagca agcagaaaaa
    23281 gctgtctcag aattaacgaa ccagacaaac aattatatcc aagcaaaaga agagcaaaca
    23341 gcggcttggg cagaacgatg gaagcttgcc gatgtggtca tcgaaggaga cgaagaagca
    23401 caacaaggta ttcgattcaa tttgttccaa ctattctcta cttactacgg cgaagatgaa
    23461 cgattgaata tcggccccaa aggattcaca ggagaaaaat acggcggagc cacttactgg
    23521 gatacagaag cgtatgctgt ccctctttat cttgctttag ccaaacctga agtaactaag
    23581 aatctgctga aatatcggca taatcaactg ccgcaagcaa tccacaacgc tcagcaacaa
    23641 ggattaaaag gcgcgcttta tcctatggtc accttcactg gtgtcgaatg ccataacgag
    23701 tgggaaatca cattcgagga gattcaccga aatggcgcga ttgcctatgc aatctacaat
    23761 tatacgaatt acaccggcga tacggaatat ttgaaaaatg aaggactgga agttttagca
    23821 gaaatcgcac gcttctgggc agaccgtgtc catttttcaa aacgagcaaa taaatacatg
    23881 atccacggtg tgaccggccc aaacgaatat gagaacaata tcaataacaa ttggtataca
    23941 aatacgatcg cagcttgggt cttgcgttac acgcgagaaa attatttaac ttatcaaaaa
    24001 gaagctgcta tcactatcag cgaagaagaa ctagccaaat ggaaagatat cgcagagaac
    24061 atgtacttcc cagttgacga agaattagga atctttgtcc agcatgacac attcttagat
    24121 aaagatctca tgcctgtatc cgatattccc acaagcgatc ttcctctgaa tcagcattgg
    24181 tcttgggaca aaatccttag atcttgcttc atcaagcagg cagatgtact acaggggatt
    24241 tatttcttca atgatacttt tacaaaagaa gaaaagcaaa aaaactttga tttctatgaa
    24301 ccaatgactg tccatgagtc ttccctttcg ccaagtatcc attcgatttt agcagcagaa
    24361 ttaggaatgg aaagcaaagc tgtagaaatg taccagcgaa cagctcgctt ggatttggat
    24421 aattacaata acgacacaga agacggcttg cacatcactt ccatgactgg tagctggttg
    24481 tcgattgtcc aaggatttgc tcaaatgaaa actgcccatg atcagttgag attcgcacca
    24541 tttctgccaa aaacttggaa agcttattct ttccatatca attatcgcgg acgtttattg
    24601 ttcatctcag tcacagatga acaagttact ttcgagttat tagaaggttc tcctctagaa
    24661 atttacgtct atgaacagtc ttattcttta gcggatcgtc tagaaataac gctaagaaag
    24721 gaggaagcaa atgtttaaag gggtattgtt tgatttagac ggtgtcatta ccgatactgc
    24781 tgaattccat tatcgtgctt ggcgaaagct tggtcaggaa atcggcatta cgatcgacca
    24841 cacattcaac gaacagctaa aaggcgtcag ccgagaagac tcactctctt tgcttttagc
    24901 ttatggcgga aaagaacact ctttttcaaa ggaagaattt gcagaattag cgaaaagaaa
    24961 aaacgattat tatttagaga tgatccagac gatcgaacca aaagatgtct tccccggggt
    25021 cgtgccttta ctggattctt taaaagaaga aaaaatcaag attgctcttg cttctgcaag
    25081 taaaaacggg ccattcctac ttgaaaaaat gggtcttaca ccttactttg atgctattgc
    25141 cgacccggca gaagcaacaa acggaaaacc tgcgccagat atcttcctcc ttgcagcaaa
    25201 agctgtcgga ctaaaagcag aagaatgtat cgggatcgaa gacgcgcaag ccggtatcca
    25261 agccatcctc tcaagtggcg ctcaaccaat tggcgtggga cgtgctgaag accttggaga
    25321 ggagatcccg cttgtccctg atacgacttt tcttacgatc gattacttga aagaaaagtg
    25381 gcatgaccat ggataatcca gagatctccg tcgtaccagt tccagagact gaaataaaga
    25441 aaatcacatt gaagaacgac tacttagaag tcgttcttct caattacggt gcccgcctgc
    25501 accaaatctt cgcaccagac aaagaaggaa aatcagaaaa tgttttattg tcttacgatc
    25561 gtttcgaaga tgttctaaca gacaaaagct tttttggagc aactgtcgga cctgtagccg
    25621 gaaggatccg ggatgcttct tggggtgagc atagattgga gaaaaactgc ggatcacacc
    25681 atatccacgg tggcacgaaa ggctggtctt tccaattttg ggaagtagaa gttttcaaaa
    25741 ctccatcatc aataggtgtg gtcttttact taaaagatga attttctacc tatccaggac
    25801 caatcactgc tacgatcact taccgtttaa caaaaaacga attagagatg acaacctctg
    25861 ctagtagcct tgttgaaacc atctgcaatc cgaccaacca tgcctacttc aacctttcag
    25921 gaaatggaac aagagacatc tatgaacatc agttaacggt ctttttagat ggtgtgcttg
    25981 aattagatca agaaaaactg cctactggaa attggacaaa aaaagaaaaa ctgccaatcg
    26041 actttagaaa atccccaaca ctgcaagaga tccttgcatg ttatccagat ggactagatg
    26101 atgtatttct tttgcatcat cctaggcttt ccaaaacttc cttacaatta ttcgagaagc
    26161 attctggtcg ccaaatgact atagcaactt ctaataaaag tatggtatta ttttctacga
    26221 ctggattcga agctgatttc tctgtcaatg gaaaacagat gcattcaaac tacggattag
    26281 caatcgaacc gcaagaaatc cctgatatcg ttcatttccc caaatttgga tcgatcaacc
    26341 tccatcctgg tcaagaacgg atcagtcaga cgatttatcg gttctctgca caataacaag
    26401 agaaaggtgt ttttatcatg gctattaccg taaaagatgt ggcgaaaaaa gccggtgttg
    26461 ccacctcgac cgtctctcgt gtcatcaacg atcatcctag tatttccgag acaacaaaga
    26521 agaaagtccg aaaaattatg gatgaactag gttatgttcc taacatgact gcccgcaatc
    26581 taggaaaacg catttccagt gccatcggcg taatcttacc ccctcttgat tcaaaagagc
    26641 ggatcggtaa cccattttat cttgagatca tggaagcaat caacgaagaa gcgcggaatt
    26701 atgatatgac tacagcaatc gctactgcta aaaattttga attgctgtta gagaacgtcc
    26761 agcgtatgca tttacaaaaa caagtagatg gttttattct agtgtattca gatagtcaag
    26821 atactgtcat tgattattta taccaaaacc acatcccttt cactttgatc ggacagcctt
    26881 atcatcatga gaacgaaatc gtttatgtag ataatgataa ccagcttcta ggtaaacaag
    26941 caacagaatt tttgatccag aatggacata agaatatcct gtttgtgaca aacacgactc
    27001 atgaaagttt gtatttcgaa cgatatttcg gttatcaaaa agcaatgatg atggctggtc
    27061 ttcctgcgtt atccagtgta gtactagagc atccggagga ttatctgtcc tttgagaaaa
    27121 cgttgaaaga aaacgaagcc acagcattgg tggtcattga cgatatcttc gcccttcgca
    27181 cgatgcagtt ggcgcaaatg tatggctatg tcattccaga gacactctcc gtcattagct
    27241 tcaacaactc gatcttctca acattaacac acccttacct tacaagtatc gatatcgata
    27301 tctccgaatt aggacgtgtc gctatgcaaa agctgcgcga attgatccat gaagaattga
    27361 gcagtggcgt acgtttggtc atccctcatc gcttgatcaa aagagagaca gtcgttcctt
    27421 tagaaaaccc atactaaata gctgactcac taaatacgct gaccatttat cctatggtca
    27481 gctttttatg taaaagaaga tactacttct aacacattgt ttatctgtaa aaaagagcgt
    27541 gggacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac ggcggaagca
    27601 gaagcaactc cttcggaaat aagctgaaat tcacaaaaat ttaaaaagca attttcttga
    27661 gtttcttctt atttcttgga gttaaacgct tctgtcccag cctctttttt ccttccttca
    27721 attttttaca cgtcgacgat tcaataaaaa accgcctaat attgtaaaga caggtgcaag
    27781 aatcgagaaa aaggcgaatg gcaagtaatg caacgcgcta actttcatcg ttcccataat
    27841 gaatgtgccg ctgactcccc acggtacgag agaattgacg gtcgcacctg aatccgctag
    27901 tgttcgtgtc aaataggttt ttgataagcc aacgtcatca aaagaacgct tgaatgtttc
    27961 tcctggaaga atgatggaca gatactgttc tccaacgatc agattgattc cgatacagct
    28021 caaggcagta aacccgacta aacgagaagg tcggtccatc ttttctttta attcttgaac
    28081 gatggtatca acgatcagat atttgatcag taacccacct aatgccaatg caagtaaaat
    28141 caaactagca gaacctaaca tactcatgat cccgcctcga gaaagcagtt catctagctt
    28201 tggatcaggt gtatttgcga cataaccatt catcaagatg tctgctgctt ttccgaccgt
    28261 caaacttttc tcatggataa aagaaagaac gagagcagct gtcgatcccg ctagtaatgt
    28321 agggatagcc ggaatttttt tgattgcccc cccaaataaa ataactagtg gaatcaatgt
    28381 ccaaggagag atccaaaagc tcgctttcat gttttggatc atttcattga tactagctag
    28441 atcggctgaa tgactgttat gtcctaatat cgtataaaaa atcaacgaaa ggacgaatgc
    28501 tgggaaatcc gtataacgca taccagcgat atgttcgaat agattaactc ctccaatacc
    28561 cgctgccaag ttggtcgtat ctgataaagg cgagcagtta ttccctaaaa aggcaccgga
    28621 aacgactgcg cctgtagtca ttgcatcatt gaatccaagt aaatgaccga tccccaaaaa
    28681 agcaattcca atcgtcgaaa tcgttgtaaa tgaactgccg acagtcactc cgacaattgc
    28741 acaaacaaga aatgctgtcg gtaaaaagaa acgcacagaa acaagtttaa atccataaac
    28801 cataatcgta gggatggttc cagataaaat ccatgaagca actaagacac caatcaaaag
    28861 aaaaatcagg attggaatga tccctggagt gattcctttg atgatcccgt catggatctc
    28921 atcccatgtg aactttttaa atctgccata aaacaacaaa cacatgaaag ctgcaaggat
    28981 cgggatatgt ggcgagagct ggaatccgat gatcagtact cctaggagtg ccaacaacac
    29041 gatcaataca gcgatacttt cttttaaaga aatattttta ttcatcgtcg atctcctatt
    29101 tcaaatgaaa aggccctgcc atgaaaaaac tattcacttg gcaggacctc ctactaaatc
    29161 agcgacaaaa cgtcggctga aagacttatt tttttatttt tcttctaaac ttttttctac
    29221 ctcaatcccc caagaatctt tcttgaagtg attgatgatc aatggaataa cgaacatgac
    29281 cactgtgata gctgctacta ataatgcatc actcaatgtc gctgtaccta atgtagcaaa
    29341 tacgacaacg atgatagaga agatcagcat gcagctgact acccaagcta atccgtttcc
    29401 gtttttacca attcggtaag gacgttcgaa ttctggatgt ttcttacgca ttttaagcaa
    29461 tgaaacagca atcaaaatgt acacgataca ataaataaca gttgtagcat ttgtaagtgt
    29521 caagaataca gcacttacgt catccattac accataaagc aaagcaaata atgaaataac
    29581 gattgattgt gttagaacga catttctaga tacgccgtat ttgttttctt tatggaatcc
    29641 gaattttggt ggaaggaatc cttcacgagc tacttggatg attgtttttg atggtccagt
    29701 aacccaagct gataattgta aaagcacacc aataaatacc atgaagctga agatatttcc
    29761 gatcacttct ggtaagccta ggatttgaca gtcaatcaag ataggctgcg tgatatttgc
    29821 taattccatt tttccatctg gtacgacatt tgctacaagc atcgcattga tgacattcaa
    29881 taaaactaat ccgatcaaag cgataaatac gccttttgta taattttttg ttgcgtcttt
    29941 caaacgaggc atgtaaacag aactcatttc gatcccaaca aaaataaatg tgataccagc
    30001 taagtatttc aatgaatcaa tatgttccaa gtcagggaaa gcttttgacc atgaaaagtc
    30061 acctagatag ccgtttgaga ctagtccgac tttgaaggct gccaaaacac ctaaaacgaa
    30121 catgatgact actgggatat atacccctag ccatacaccg atgttcccgc cgacttttgc
    30181 catatcgaat ttcaagttca agatcgtgat gatccagtag ataactaaaa tacaacctaa
    30241 aacgaaccag tgattattgc caagttctac attaccaaat gtgtttccta acaatggtcc
    30301 taaagtagaa gcgaccatta ccatacctgg gaacatttgc acccataata gccatgcaac
    30361 gacaaatccc catttgctgc ccagtgctgt ttttacccat aactgaggac cgccttcttg
    30421 aggaagcatc gtggaaagct caccggaaac cattgatatc ggaccggcaa aaaacaaaac
    30481 tgcaaaaatt gaataaaaaa tcaatgtcca accgactgcg gctaacgtag gaatactccg
    30541 aaccgttgca cacagtgcca ttgtcagacc gataaaggtc ccaagaccca ttttacttac
    30601 ttcagattta ctcaccgttg acactctcct tgattttttg gataaaataa cggtgtcttt
    30661 gaagtagttt cagcaataaa cgaaacaatt cagcgaatat ttgaggggat tctttgagtt
    30721 tattgcttga agggtttctt caaagacaac ctcaaaacaa agggatcgaa gagcttaggt
    30781 ctccccttgc tcttcagatc caaacatgcg tcagaaacag ttagtattca agctgttttc
    30841 taacatgatt accttgctta ttttgcttcg cttgccaata attggtctgc gtagattttt
    30901 tccaattttt cttgaagagc tgctttgatt ttttcagcat attcttcaaa gtgttcttct
    30961 ttgttcatgt atggtgtcat tacgctggca cgcaatacag ttacttttcc ggcacggttc
    31021 cattcttcat ctgagaatcc tagttggttc acaaattgta atgggctgtt gccatagtct
    31081 gggatagcaa agtcagtatg tgaagttaag aactcattac cgtaaatgct tcctttcacg
    31141 tatgaagaat aatcatatac gtcatggttc aatttattca ttgcaactaa gtcatcgttg
    31201 cctttttctt tgaatacata gtctaccata ttgaaatctg gataagttaa tggatgaact
    31261 tcgatttctt tgtctcctac tttaaatgac aagtcattca agaagttgta gaaacgatgt
    31321 gcaccttcga ttgatgcacc cattaattta ccatatcctg tgacattcaa aggtaagaca
    31381 tggtgtgctg cccaaacgct ggctgctgtt gcccctgctt ttgaaccttc caagatataa
    31441 gccccaagta atgctgggat atctgcgcct ttttcaaata catatgtcgc aaagtaagag
    31501 ataacgtctc tcatgcggat atctttgatg acgataccac cggcagaata tggaacatag
    31561 cccattttat gtgggtcaat cgttacagat tctgcttctt cgattgcttt atatgcactg
    31621 tgtacttctt ctaagatata atctttgttt tctgtaaaga cattgtattt gtaatgaaca
    31681 tcttttaaat cttcaaatgg aatgaagttg ttgtcttcgt ctaagaaaat cgcacggccg
    31741 tatccgccat aagcagcatc tacgtgtaag tagaagtaaa taccgtcttt ttctaatacg
    31801 cgacgcaatg caacgatttt atcgataccg tcgattgctc cttcttctgt tgatcctaca
    31861 acaccgacta cacctaggat tggtgttttt tcagctgcta gaccacgaac gattttttca
    31921 agttcgttga tgtccatgcg atagttgtgg tcaactggca ctggaatcac ttggtctaaa
    31981 ccgataccga tgatgtcggc agcttttagc catgaatagt gtttagtttg tggaaccaac
    32041 cattttccta atttttctaa atgttttccg ctacgtgctg agtgagcttt gatttcgtcg
    32101 attttttctg ggacgctgtc taacagattc atgatttctt ctgttgacaa gttcattaat
    32161 tcccaatcgc tttttccagc aactagttca ggtgtcactt ctttcattgc aagcggcaat
    32221 gatttgatgt tgcgggcata ccaaagtcct tctaagtttg ctaaagaacc atcagcaacg
    32281 atgtggcccc agccatcttt atagctcatt aattttgcaa attccattcc tacttcttct
    32341 tccatttggc ttgttgcagg agaagattca taggcaacgt tattaccatt ccatagcata
    32401 gcaaaattgt acgctagtaa agatggcatc aatgtttcag agttcatatg accccagtaa
    32461 cgtccggcat tatgccaagg aacggaatgg gtacgcatcc gcgctgaaat ttctgataga
    32521 acatcctttg ttttattcac agtgtgttca aaactagcac tgcttttttc ttctggcgta
    32581 ataataggca tgtcttgagg catgtagttt tgacgccagc ctaaatgttc atccaccaat
    32641 tcattcaaca aagctttata gatttggccg ttctcagctt tgtccccaat gaatagggca
    32701 tttaggttta gatctttcga caatgattca ctcatagtga aaccctccta aaaaaatatt
    32761 taaattttgt cgagccctac attacacctg ttcttctatt ctgtaaacta agttttggcc
    32821 tgatttttaa tcatatttta atatgtatca attatccgaa ttgttcacac ttttgaaata
    32881 attaaaaaaa acgagggaat ttacaaatgt aaactccctc gcaaagattg tattaacgac
    32941 aaccagcgtt ctattttagg atgggtttca aaattggaat gttgtaatag tatttccctt
    33001 tttcaaggta aaaacttact aaattctaat caattacttt ggccaaaaag taatttttct
    33061 taccttttcg aataaccaca aacttaccat cgaattcatc ggatggattt atgacaaaat
    33121 ttaaatcggt aacgcggtca ccgttaatac ttatagctcc gtttgaaaca tcttcacgtg
    33181 cttgacgttt agatggttca atttttgtgg aaacaagcag ttccacgata ttttcaggtg
    33241 tgcttgaaat ttcaacgttc ggcattttac cgaatccttg cgcgatttct tccgcattta
    33301 gttctttaat atttccagag aacaatgctt gcgttatttt ttgcgcttct ttcaagtctt
    33361 cttcactatg cacaaatctt gtaacttctt ctgccaaacg gcgttgtgct tcacgtttct
    33421 caggttctgt ctctactttt ttcgctaagt cttcgatttc ttcttgagac aagaaagtaa
    33481 agaatttcaa gtatttcact acatcgcgat catcttgatt caaccagaat tggtagaact
    33541 caaatggtga agttttcttc ggatcaagcc aaacggcacc gcctgcagtt tttccgaatt
    33601 tcgttccgtc agccttcagc atcaatggga tcgttaaacc gaacgcttct gcttctggtc
    33661 cttctaattt tcggattagg tctaatccag ctgtgatatt tccccattgg tctgctccac
    33721 cgatttgcaa ttgcacatcg tatgttttat gcagatgcaa aaagtcgata gattgcagga
    33781 tttggtaagt aaactctgta aatgagattc ctacttccaa acggctggca acgatatctt
    33841 ttgctaacat cgtgttgatg ttgaagtttt taccgtagtc tctcaaaaat tcaagtaaag
    33901 agatttttga caaccagtcg tagttgttca caaatgtgat atttgcatct ttaccaaaca
    33961 attttctcat ttgattggaa agtgcgtcca cgttatgttg cacttgttcc atcgtttgca
    34021 acacacgttc tgttttgcgg ccgcttgggt cgccgatcgt tcctgttccg ccgccaatca
    34081 gaatatatgg atgatggcct gccaattgga atcttttcat catcataaat ggaatcaaat
    34141 gtccgatatg catcgaatca ccagttggat caacgccgca ataaagtgaa atgctctttt
    34201 cttcaacgag ctcgcgaagt ctttcttcgt ttgtttgctg attaatggcg tcgcgccaag
    34261 ttaattcatc aataatattc attttactga cctccgtgta gtttttttat gaaagcagga
    34321 acctgctagc atatgtaatt aattatttct tcaggatagg ataggaaata aaaaaagtcc
    34381 cttggcatat aagccaaggg acgaaatctc gcggtaccac ccaatttgta tgtctagata
    34441 tccaacatac ctctcgaaca tttaacgctg tcgcgtggca atcactgcca aagactcctg
    34501 aaatgtaatt cgctttaaag catgtactgg tttgcagcga ccaccagctt tctgaaacat
    34561 tggctttaac aactacttcg tttcgatctt cgtcttgtta ttattaatat gtgtctatca
    34621 tagagcgcaa ctttttcttt gtcaacagaa aaaggatatt ttttttaaat tcctcatttt
    34681 ttcttttttg aatccgctct aattccgaac attttatttt caattattgt taaaagaaat
    34741 aactttttct gtattttttc tgcaatttat cgagaagtca ctttactttg cctcttattc
    34801 tttcttcgtt acaataagga atgggacaag taaaaaaatg aaaaagcaag ggacgaggta
    34861 tatggaaaaa aagcaaaaaa ttattttagg agccacttct gctttaacta tcgttatttt
    34921 aattctaagt ttgttttttg tcatccgatc atctacaaaa aaagaaccaa tcattcctga
    34981 aaaaaaaata acagaaaaag caaaagaacc gacagatgat aagcaacaac tagttactca
    35041 agcaaaaaat ctggctcaaa gctacgatta tgaccaagcg attgcgttat tggaaaaaga
    35101 tagtaagaac ggatctactg aaacacaaca gcttcttcaa acactaaaaa aggagaaaga
    35161 acaactgatc gactggtctg atcctactca gatctctcat gtctttttcc acagcttgat
    35221 cgtggacccg caaaaagcct tccactctca acaggcacaa ggatacaaag attatatggt
    35281 gactgttgaa gaattcaacc gttcgatcga acaactttat caaaatgatt ttgttttagt
    35341 taatttgaat gatttgatcc aaaaagacga acaaggaaat ctttccttta acgggcttgc
    35401 gctacctgaa ggaaaaaaac cattggttct ttcacaagat gatgtcagct attatgaata
    35461 tatggataat tccggttttc ctagccgatt ggtcgtatca aataacaatg aaatcaaaaa
    35521 tgtctataaa gagaaagata aagaagaaat cggtgacttt gacatggttc ctctgatcga
    35581 tgcctttgtc aagaaacatc ctgatttttc ttatcgaggc gcaaaaggta ccttggccct
    35641 gacaggttac aacggagttt taggttatcg aacatcaaaa tctcaatatg gagacaatga
    35701 aaaaacaaaa aaagagatta aagaagctaa aaaagtggcg gaacagttga agaaagacgg
    35761 ctggagcttt gcttcccata catggggtca cttgaatatg actcaagctt cactagaaga
    35821 tgtaaaaaaa gacaatgaat tgtggcagca agaagttgca cctatcctag gaaaaacaaa
    35881 tattctgatt tatccatttg gtgcagacat cagcgactgg cagccttatt cagcagaaaa
    35941 tcaaaaattt gcttacttga agagtcaagg tttcgatatt ttctgtaatg tcgatgcttc
    36001 tacaccagct tggggacaat taggtacgga tttctatcga aatgctcgga tcaatatcga
    36061 tggcattcgt tttcaatctg atttagacgg aaaaaatcct attcttgatc cattcatcaa
    36121 tgtccatgat gtctatgaca aagaagcacg cgaatctgct tgattttacg tcatttatga
    36181 accaaaaaag ccgaactgac attttatcgt cagtttcggc ttttttatgc tttatttctt
    36241 cttcggtgcg attcccttga tcaggacttc tgtccattct gctgtatatg gaagcagttt
    36301 tgcgtatatc tccggcttag aaattccttc taccattgct tgaaaataga ggatcaagaa
    36361 ctcatccgaa tatttcaaat ccacttttcc ttctttccgc cctcgatgga aaagatcaag
    36421 catgatactg aaactttctt gcgtatacgc atccatcgcg tcagaaaatt cttttttttc
    36481 tttttttgta tagtatgtca tcaaatcgat ataaaattgt ttgttcactc gattgagata
    36541 acggattttg ttttcactca tggcaatcag tgtttcttca aatggaagat tttccgcaat
    36601 caacgctttc gcttcttgcc ctaaatcatt cataaatgca aaaaatactt cataaatcag
    36661 cccttccttg ctgccaaaat atttaaaaat cgtcgcttta cttacctgag cgttctcagc
    36721 taccttatcc attgtcactc cagcggttcc tatgtctgtg ttcatcaagt caaaagctgc
    36781 ttgtaaaatc tctttctttt tctcttgtgc tctcttttca aaaccgttca tttgtagagc
    36841 ctcccgtaat t
//
LOCUS       DACCYH010000019        36696 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.019, whole genome shotgun sequence.
ACCESSION   DACCYH010000019 DACCYH010000000
VERSION     DACCYH010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.019"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            229..699
                     /locus_tag="IVY85_000914"
     CDS             229..699
                     /locus_tag="IVY85_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP8043948.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            921..2024
                     /locus_tag="IVY85_000915"
     CDS             921..2024
                     /locus_tag="IVY85_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8043949.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            2308..2988
                     /locus_tag="IVY85_000916"
     CDS             2308..2988
                     /locus_tag="IVY85_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8043950.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            3078..4112
                     /locus_tag="IVY85_000917"
     CDS             3078..4112
                     /locus_tag="IVY85_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8043951.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            4126..4461
                     /locus_tag="IVY85_000918"
     CDS             4126..4461
                     /locus_tag="IVY85_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043952.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            4445..6925
                     /locus_tag="IVY85_000919"
     CDS             4445..6925
                     /locus_tag="IVY85_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043953.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            6981..8540
                     /locus_tag="IVY85_000920"
     CDS             6981..8540
                     /locus_tag="IVY85_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8043954.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            8537..9901
                     /locus_tag="IVY85_000921"
     CDS             8537..9901
                     /locus_tag="IVY85_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8043955.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            10072..11142
                     /locus_tag="IVY85_000922"
     CDS             10072..11142
                     /locus_tag="IVY85_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8043956.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            11157..11825
                     /locus_tag="IVY85_000923"
     CDS             11157..11825
                     /locus_tag="IVY85_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8043957.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            11845..12423
                     /locus_tag="IVY85_000924"
     CDS             11845..12423
                     /locus_tag="IVY85_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8043958.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            12824..13735
                     /locus_tag="IVY85_000925"
     CDS             12824..13735
                     /locus_tag="IVY85_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8043959.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(13823..14740)
                     /locus_tag="IVY85_000926"
     CDS             complement(13823..14740)
                     /locus_tag="IVY85_000926"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP8043960.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            14942..15379
                     /gene="zapA"
                     /locus_tag="IVY85_000927"
     CDS             14942..15379
                     /gene="zapA"
                     /locus_tag="IVY85_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP8043961.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            15438..15986
                     /locus_tag="IVY85_000928"
     CDS             15438..15986
                     /locus_tag="IVY85_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP8043962.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            16101..18461
                     /locus_tag="IVY85_000929"
     CDS             16101..18461
                     /locus_tag="IVY85_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP8043963.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            18548..18862
                     /gene="trxA"
                     /locus_tag="IVY85_000930"
     CDS             18548..18862
                     /gene="trxA"
                     /locus_tag="IVY85_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP8043964.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            19044..20822
                     /gene="uvrC"
                     /locus_tag="IVY85_000931"
     CDS             19044..20822
                     /gene="uvrC"
                     /locus_tag="IVY85_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP8043965.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            20967..21866
                     /locus_tag="IVY85_000932"
     CDS             20967..21866
                     /locus_tag="IVY85_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8043966.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            21859..23121
                     /locus_tag="IVY85_000933"
     CDS             21859..23121
                     /locus_tag="IVY85_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8043967.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            23282..25180
                     /locus_tag="IVY85_000934"
     CDS             23282..25180
                     /locus_tag="IVY85_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP8043968.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(25510..26229)
                     /locus_tag="IVY85_000935"
     CDS             complement(25510..26229)
                     /locus_tag="IVY85_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP8043969.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            26566..26898
                     /locus_tag="IVY85_000936"
     CDS             26566..26898
                     /locus_tag="IVY85_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043970.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            26885..28888
                     /locus_tag="IVY85_000937"
     CDS             26885..28888
                     /locus_tag="IVY85_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP8043971.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            28907..29377
                     /locus_tag="IVY85_000938"
     CDS             28907..29377
                     /locus_tag="IVY85_000938"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP8043972.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            29398..29982
                     /locus_tag="IVY85_000939"
     CDS             29398..29982
                     /locus_tag="IVY85_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP8043973.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            29997..30983
                     /locus_tag="IVY85_000940"
     CDS             29997..30983
                     /locus_tag="IVY85_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP8043974.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            30973..31284
                     /locus_tag="IVY85_000941"
     CDS             30973..31284
                     /locus_tag="IVY85_000941"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP8043975.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            31306..33087
                     /locus_tag="IVY85_000942"
     CDS             31306..33087
                     /locus_tag="IVY85_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP8043976.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            33080..34456
                     /locus_tag="IVY85_000943"
     CDS             33080..34456
                     /locus_tag="IVY85_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP8043977.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            34463..35098
                     /locus_tag="IVY85_000944"
     CDS             34463..35098
                     /locus_tag="IVY85_000944"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP8043978.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            35128..36483
                     /locus_tag="IVY85_000945"
     CDS             35128..36483
                     /locus_tag="IVY85_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP8043979.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
BASE COUNT        12160 a         6282 c         7879 g        10375 t
ORIGIN      
        1 tcaaaagaaa tcaaaatagc ggtatttgtt ctttttatat caacaatcct tgcttgatta
       61 ctatgcgtat ctcttatatg ctaaagaaag aaaagtagaa gaataaagga tatgttagat
      121 agataaatag aatctctgaa gaataaatca cgtgatcgtg acgataaaat acacttacgt
      181 caggtgataa aaaatacata agtagaaaac taagaggagg aacagaaaat gaaagaagtt
      241 caattgatca gaaaaagcga actatcagaa ggtggttgca atgcatgcgg cgtagtagaa
      301 gcaacaagct atacattaaa gcttggtgcc aacaaagcga taatctctga gttgacagta
      361 ggcggtcttg tggacagtct ggcgcttgct gaaggtttta ttggagaaga tatttatgag
      421 atgttttcag aaatccgtca gttaaaaaaa ggggaaaact gcatagaggt tcatcatgag
      481 agcccgaatg tgagattcaa acgtggtgat aatgagatga tcttcaataa tcatgtgtcc
      541 gatcacaccg agctatatgg aatcgtgaat caaatactaa ctgagctttt tggattggga
      601 ccttatgcgt tcaaggaaga aaacggtaat ccaaaattaa acgaagaatg gcaagaaacg
      661 attgaaatac aaagaaacaa cccgcactta tttcaatagg catccacagt atttcgtaaa
      721 agggactttg acacgacttt tgtcaaagtc ccatcgtttt ttatctattt gtgttctaaa
      781 catttctttc agagtagtaa caactggaat gactgaaatg taaacagatt atttgcctgt
      841 atcaatacgg aagagtaata taaagtgtgt aaacgctatc atattattca ttcaattttt
      901 tatagaggga aggcattgaa atggaaaaaa cgtatcaagc aggtacaaat gaaggaatag
      961 tggattttat caatatggaa gatttagaaa tagccgcttc acaagtgatt ccagctggtg
     1021 gttatggtta catcagcagt ggtgctggcg acttatttac ttatcaagaa aatgaacggg
     1081 cattcaatca tcagctgatt attcctcatg tattaaaaga tgtagaatta cccgatacaa
     1141 ctacacattt tgacaaagaa acgctgactg caccaatcat catggcacca gttgctgctc
     1201 atggcttagc tcatgtgaag gcggagaaag cttctgcaaa aggtgtagca gattttggga
     1261 cgatctatac agcaagttct tacgcttctt gtacattaga agagataaga gaagcaggcg
     1321 gggagaaagc accgcaatgg tttcaatttt atatgagcaa agatgatggg atcaatttgg
     1381 atattcttga agtggccaag cgtaatggag caaaagcaat cgtattgaca gcggatgcaa
     1441 cagttggcgg aaaccgtgaa acagaccgca gaaatgggtt taccttccca ctgccaatgc
     1501 cgatcgttca ggcttatcaa tcaggagtag gccaaacgat ggatgctgtg tacaaatctt
     1561 ccaaacagaa gcttagtcca aaagatgtag aatttattgc tgctcattct gatttgccag
     1621 tctatgtgaa aggtgtccag tcagaagaag atgtctatcg ttcattagaa tctggtgctg
     1681 gcggaatctg ggtatctaat catggtggcc gtcaactaga tggtggtcca gcagcgtttg
     1741 attcgttgca atatgtggca gaagcagtag ataaacgtgt accaatcgtg tttgacagcg
     1801 gtgtacgacg cggacagcat gttttcaaag caatcgcatc aggtgcagat cttgttgcta
     1861 ttggcagacc cgttatttat ggattgtctt taggaggaag tacaggagtc catcaagtgt
     1921 ttgatttctt taaaacagaa ttggaaatgg tcatgcaatt agctggaaca caaacagtag
     1981 aagatatcaa aaaaataaaa ttgcgtgaga atcgttttat ttaagaaaca aatgacgaaa
     2041 aaaataggag tgcggcagat gatgccgtac tcctattttt atagttcaag gatgtcacta
     2101 atagaataat ttgaaacgaa aaaataaaat tgaaatattt tttcatactt aataaaaaag
     2161 gaagaatatt catgtaaacg attttattgt ttttttctct tttatacaaa aagggtcata
     2221 atttcaagta agcggatgaa ttcatgagga ttttttgcat actgtctcta ctatacttag
     2281 gtcactaaga aaaaggagtg atttattatg aaaaagaaca tgattgggac attattactt
     2341 tcaacacttc ttctaggcgg cttggcaaca ccagcttttg ctgagggtca agcgacttca
     2401 aaaggagata ttacctttac tgaaccaaca aataccgtgg agcctcttaa ccctactgat
     2461 ccttcaaaac cagtagagcc agcagatccg gaaaatccag caactggcca gacaggtcct
     2521 ttaacactgg atgtagttcc cgaattgcca tttggtacac acgagattga aagcggaaca
     2581 aaaacgtatc aagtagacgc ttcaaaaaac gatacacctt atctccaagt atcagatcgt
     2641 cgaggtgtag gtgcagatgg ccaagcacaa gggtggaacg tgacagtttc ggtttcagat
     2701 tttgtaaacg gttcacaagt tttgcaaggc gctgaattag attttggaac atccactgtt
     2761 aaaagtacgt cagataacga atccactgga ccaactagtc aaactgtaac tgggttgagt
     2821 aaagcaagtg cagcgacacc aatatttaca gcagcaaaag atcaaggtct gggaacatgg
     2881 ctttctgttt atgatccagc aaatatcaca ctgaaagtgc cgaaagcagc agctggaaca
     2941 ttcacggctg atttgacttg gaacctcgtg gctggcccag tagcatagag tatgaaataa
     3001 attcaacaaa ctgcggtgaa aatactatag gtgaaggatt ttttaccgca gtttttgttg
     3061 aaaggatgtg gtggacaatg aatcaaagta aacgactgtt tgtttcgttt ttttctattt
     3121 taagtctatt tttcattttt ccgtcaatca gccaagcaaa ggattcagca ggagactttg
     3181 gaatcaaacc ggtatttcca gagaaccaaa tagataaagc aatcggttat tttgatttat
     3241 tagtggcacc ggaacaaaat cagacactag aagtaattat cagcaattct tcagatgaag
     3301 aacgaacatt tgaggtctct gtcaatccgg cggtaacgag tgatggtgga acgatcgatt
     3361 atagtcaaaa aaacccaacg ttagatgaga cattgccgtt tgatgtccgt gacgttttgc
     3421 tcattgcgaa aaaggaaatc aatgtatcag cacatgcaga aacaactgta cctatagaag
     3481 tgaaaatccc agcaaaaagc tttaagggac gtgtattagc gggtattcat gtttcaccta
     3541 aagaagaagc tgaaacagaa aatgctacag aaggagcaca aatcaaaaat cgtattgctt
     3601 ataatctcgc agtcgtttta caagaaagtc aagagacgat agaaccggat ctgaaacttt
     3661 tatcaggaga tttggacgag gtcaacgcaa agccaactgt tcagcttcgt ttccaaaatc
     3721 ctcagcctag gattatcagc aatttgatat ttacgagcaa gatcttttat gaaaatcagc
     3781 tttatattga aaatacgtct aatgcatttt tagttgcacc aaattcgaat tttcacttga
     3841 atcttgattt agctggtgat aaagcaaaag ccggcgatta ccgcgcagaa atcatagcaa
     3901 aatcaggcga cagtaatgaa tggcgattta cacaaaactt tacgatcaaa aaagaaaaag
     3961 cacaaaaagt aaatgaaaat tccgtttttg cagtacaaga acaatctttc ccatggcttt
     4021 atgcaggatt ggttgctgca gttctagtgt tatggttgat tttgctgttt ttgtgggtcc
     4081 acaagaaaaa gaaaaaagga gaaacaacat gatgaggtga tttcgttgaa aacatcgatc
     4141 ttattgggag ggattattgc aagtttgctg ttcttcagct ggcaagagcc agtccaagca
     4201 gataccaaaa gttcaggtac gattacattt tatgatctaa gtccaattcc gcatccaagt
     4261 ggttcagtca atggcgcatc aaaacagcag acacagctac cgatttataa agaatctaat
     4321 caacatggcg tattcccgga gacgaatgaa caaaagatcc gttttcttta tttgatcatt
     4381 ggtatagaat tatggcttct tgcgtggttg ttgttatgga gaagaaagga aaaggaggat
     4441 gaacatgttg ctacaaaata aattttcatt ttttcgctcc ttctttttta cttcgctttt
     4501 tgttttaagt atatttatct tttcaccttc ggcagaagct gttggagcaa cgcctcctgc
     4561 catttcttat gatccataca cgtatgaaaa tatcgtacct ccaaatggtg gacgtagtgc
     4621 agtcaatggc gggaagcttg tccaactaac tggggctaac caattgccta aattgactgc
     4681 tacttatttg aacgatggtt caaatgatcc ttggaaaggt ggcacggtcc gttttcattg
     4741 ggaaagccat gtgaaactaa caaatgtact tgatttggtg aatatcactg cacaaggatt
     4801 tgcatttgtg gcgatcatca atttgccgga acatgtatca agcagtgatg tattaaaagc
     4861 aattgactgg aacaacgcct acttgtatat tgccggtgcc cccatcaagg ttactagcgg
     4921 aagtttatta tcagcaggaa gccatacctt gcgttttggg atggggtcgt gggatacaaa
     4981 tactctattg acagatatta ttgctattat tacgcgaggc gaattcaacg tggacgatat
     5041 cccgcttcaa tttgatgtta cagctaatgt cgcgaagatg acagaaaacg gagataaata
     5101 tgatacgagt ccaaacaaaa ttttgaccaa gggtaaatta caaccttcta tgtctaaaaa
     5161 ggccgttttt tctgttgatt tttatgattc gaataatatt atccaagatc gtatgttcaa
     5221 ttcgatttta gctccagctg caggtagatt ttatcctaaa ttaactggcg gaaacttgaa
     5281 tccaggagca tatgcatttt ctgcaagtgc agagataaat acgtggaata gttacatctc
     5341 accaagtgat tcaacaggaa aatataatac atttgctgat accacagaat acgtggatgg
     5401 aaataaccag aaccttccag gctctaccaa tagtcggaca ttgacgatgg ggctgactga
     5461 aggacgtttt gatcagttgc catttagtcg ctttgatcgc gtagtcaact attttacagg
     5521 aaaaaacgtt acacaaggag caatactgac tcatactcca gcaagcacaa gtggattgaa
     5581 tcagacagcc aatatcgtgt acagtgggaa agatgcgcaa ggcggattgc tttctcctgt
     5641 tgctttaaag gtattggagc agtacaagct agacggaacg gttttaccaa caaacttaac
     5701 agccgatagt cagtggggaa agctcctttc ttctgcatcg taccaagtca cggccaattg
     5761 gtcagcaact gaactaaaaa agggttcgat ccattacagg ctttattcga aaggaaccca
     5821 aaaattagcg acaggatgga cagatcaact tttccaaacg atccaatcaa ataacggggg
     5881 tgtaaataca gctacttcta cactacctgc attggcttca gggcaatatt attttgatta
     5941 tcgcttggtg gacgatgtac ttactaacat gtatccgtct tttgcgtaca aatggcagtc
     6001 tgaacaagct ggaatcacca ctcttcctca aataacagtg gctaattttc catctgtgag
     6061 tgcagatagt cagctaaaaa accttagcag acaacctgag acaggagatc ccctgtctgc
     6121 tctatcagga gatacgattc aagaaaatgt gacattcacg ttaacaaaat ctggggatgc
     6181 gataaccgac aaaaaaatca cgatctcact tcctgaaaat actacttata tcaatggttc
     6241 cttaaaatta aatggtacaa ctatcgcaga taccggcatt caacaaggag tttctgtccc
     6301 agcagatcta ttgagcaaaa taggagatac cattcatctt acttacaact atcaattaaa
     6361 tactgtagat acttctttac aatccgtcag catactgaca aaggcagctg tactttccag
     6421 caatattaca ttagcagatg gtgctaagct tcctaaccca gttgtccaaa catctgctaa
     6481 aacaattctt gtccccaaac aagaattaac attagttaat gtaccagatg attttacatt
     6541 tggtaacgat ctccccaaac cgttgaagac aagttattat gaatcaaaag gcgatttttc
     6601 ttttgatgtg cgggatactc ggcttccaag cacgtctcct tggcaactga caggtacatt
     6661 gacttcttta tttaagaatg atcaaggtca agaattaagc ggaacaaaat tatacttcaa
     6721 tcattctgga tcaaagcaat tgattcagca aggtcaaaac acgttgattt atgagagtga
     6781 tggaacagct aaaggagagg ttctcgttga ctttcccgat accgatggtt tactcttaga
     6841 ggtaaatagc agtacgaatg ctcaacctgg cgcaacgtat caaggcatgg tcacatggaa
     6901 actgacagct gggccaacca gctaagtgat tagaatttgt atggatctgt gataactgtc
     6961 acagatcctc tttttcaagg atgagaagga aacttctgct gttaaaatca gaaatgcttg
     7021 aaggagtgga cttggaactt ttcagtttgc ttgaaaaaaa tgaacaacgg caaatcaaac
     7081 ttttccaact tttattagag gaaaaaggag aggtttccgt taataacgta cttacccgct
     7141 tatcagttga tcgggctacc ttgaaagaag atctgtttta tttacaagct aacttggaaa
     7201 atatcaaaca attggaaatt gttatccaag aagggaatat tttattaatc agacatggaa
     7261 atatagcagc agtcgatgtt tattatcatt gttacttgcg ctattctaca aaatatcaga
     7321 tacttcttta tctgttggaa aatggctcat ttgatcggca gaaacttgtc atggaactta
     7381 atatcagttc ggctactttg tctcgcagag tgaaggaatt aaatgattct ttaaatgagt
     7441 ttcatctgaa aataaaaagc gggcggctga ccggtccaga aagtcaaatt cgttattttt
     7501 atttccaatt attttggttt ggacgtccct atcttgaaaa caaaaaagaa tacagtgatt
     7561 tatcaggaaa cactttttta gatctgctgc aaaaagagtt tagttttgct attacagaag
     7621 aaggacgatt gaaatttgaa atatggcttt ccattatgaa gaagcgaatg cgctgtacga
     7681 aaaaatccaa accagattat agcaatcttc ctgttggatt gtttcaaacg acacaatttt
     7741 tattggacct ccatacattt ttgacccgtt acttctttca atatgccttt ccttggaatc
     7801 aaaatgaaac gttggttttt tatcttttca tgatcagtaa ctttacatta gatacacata
     7861 actcgcatgt ccaggagttt ttgataagcg aaaaatacaa agacgacgta gtagctcaat
     7921 tgaatcgaac gtttaaacaa tcgatagaag aatatttctc tttttatgtt tttccagaag
     7981 agatttcgca aaagattgcg ttgacagtga tgcaaaatca cgctcatttt gcctatttta
     8041 gaggatggat gaatattttt ggccaagaag gattaaaaca gcggttaact gaagtagaag
     8101 ataaacaatt cctggtttta tgtgaaagat tggtcagaca aacatttgct attgttgatt
     8161 ttactgaagt agacgaggaa aatgcccgct ttgaattatt tgctcgctat gcatctattt
     8221 tacggacgct gatcaagcag ttagaattac gtctgcaaat tgcttatgat cttccttatg
     8281 aaaggctaat ggcggatatc gtgaaagaaa tcatccagga acaattgctg aatacgatca
     8341 aatatgattt agtcgaatac gagaaagaaa agcagtatga tatcattttg actagccaat
     8401 tgaaagagta cccgtcacag aagtccgcta aagtttttgt attcactagc aacgaatata
     8461 attatgattt cccctatttg aaccaatttt taaaagaatg ttatttagaa aaattgaatt
     8521 taaggatgaa taaaacatga aaatcgtgat cattggtgct tcccatgcag gcatcacagc
     8581 tgccctaaat ctaagaaaac ttcaaccgaa agcagaagtt ttactgatcg atgaagatca
     8641 taaagatgga ctaggctatg tttcaaatgg aatcaacctt tatttaaaag gaaagatacg
     8701 tagtttagct gaagttgccc ataacatgcg gactcttcaa gaaagtgggg caaaactgat
     8761 caccgaatgg agagtaactg aacttgatcc agacaaacat cagctcatcc tcgcatccaa
     8821 agaaggaaaa aacgagacca taacctatga taaattaatc gtagcgacag gttcctctcc
     8881 tgtcaccttg tataaacaaa ttgaagctga aaatgtctac acctataaaa atctagtgca
     8941 atctaagcaa gtacttgccg ctttaaaaga agcaaaagaa gtagtgatct tcggtgctgg
     9001 ttacattgga ttggaattag cagatgcttt gagaaataaa ggatacatta ttcatctagt
     9061 tgattatatg ccgaatgttt tatcaagata tttcgataag gatatgatca acagttttca
     9121 aaatcagctg caaactaagc aaataaattt ttatccaaat gaattcttga tcgattggaa
     9181 aaaatcagaa gagaaagtag tatccgtaca acttctgtct caaacaataa aggcagatat
     9241 ggtcattttt tctgcccaaa ctcgtcctaa tacgacattg ttaaaagaaa aagtggcttt
     9301 gtatgaggat gagactgtga tagtcaatga atatctgcag acaagtgatc cggacatcta
     9361 tgcgattgga gatatcgtcc cagtcagttt tgacaaaaat aaacggcatc tctttttacc
     9421 tttagttact cgagctgtac atatggcaag agcagtagcg ctaactttga gtggtcagcc
     9481 aaccgcttac gacctcagac aaaagataac agcagcagtc attatagact actttttagg
     9541 aactgtaggg ctgacagaag atgaagcgcc attcttggaa caaaataccg gttcgtgttc
     9601 aggagaattt gatctatttc cacaatacga cgaagaaaac aaaacagtaa atgcaaagct
     9661 gatctaccat ccggatacat tagaaataat tggcggacag ctgataagcc aagaattctt
     9721 attatcagat ttgaacctgc ttgcggatat cgtgaaacac aaaactacga ttccacagct
     9781 agcagttgag gattttggat ttctgtctga atacacctct cgttttcact acctcaatga
     9841 gttagctttt aaagtactag ctaagagagc aaatagcaat agagtagata aaagactgtg
     9901 agaaatatca cggtctttta ttgtgtgggg atataataaa tattaatcaa tcgagaataa
     9961 aaaattcata taatgaaata gcggaagaat tttttgagca taatattcgc caaaaattta
    10021 tcaagtgata aaattgttgt ataaaatgat tatttcagga ggaggagtta aatgttttta
    10081 gcattaaatg aaattatgca ttcaaagtta cgttatgcgt tagttgcagg ggtgatgttt
    10141 ttgatcgcct acctggtatt ttttttgact gggttagctt atggcttagc tcaagataat
    10201 cgaacagcag tcgacaagtg ggaggcagat tcgattgtgc tttcgaaaga tgcaaattca
    10261 aatctaggga tgtccatgat tactaagaag atagcagaag aagtcgaagg tgggaaagtg
    10321 gcgtatcttg cccaaacacc tggagtcgtg actagtaaag acagtacaga agaaggaaaa
    10381 atcaatgtta gcttcttcgg tatcgataaa aatcaattca tcatgcccaa tcttgttgaa
    10441 ggaaaagctt ttgataacga tgatgaggca gtaggcgata ttagtttgaa agaagaatat
    10501 ggtctagcca taggagatac agttaagtta tctggtagtg acaagacgtt taaactaact
    10561 ggctttacgg atcatgccaa attcaatgtc tcaccagttc tctatacaac catcaatgct
    10621 taccaagaaa tccgcttcga aaaagaagat acaagtgaaa atgcacggat taacgcaata
    10681 gttgtcagag gtaaaatcag taatttacct gaagatttag aacagatcaa aatttctaaa
    10741 ttcatcaatg aattacctgg atataatgcg caagtattga cttttggatt tatgatcgga
    10801 tttttgatcg ttattgcggc tattgtgatc gggattttca tttatgtact gacgatgcaa
    10861 aagattaata tctttggtgt aatgaaagca caagggatca caggaggatt tattgcgcgt
    10921 tctgtagtgg cacaaacatt cattttatca tttgttggta ttcttcttgg tctattagga
    10981 acagtgggaa catcccttgt tctacctgat gcggttccat tccaaagcaa ttggctgttt
    11041 tttggcgtca ttagcttgtt gatgttagta gtagctgtgt taggcgcact attttctgtt
    11101 cggacaatcg taaaaatcga tccgttgaag gcaattggat aagaaggagg aagaacatga
    11161 aagcaataga atttaaagaa gtagaaaaaa gtttccaaga tggcgaccaa gtagttaaag
    11221 cattgaaaga aacaaccatg agtatcgata aaggggaatt tgttgcaatc atcggtcctt
    11281 ctggttcagg taaaagtact tttttgactt tggcaggcgg attgcagacg cctagcagtg
    11341 gctctgtatt tatcaatgat caagaattca gtaatcaaaa agaaagcgaa cgcgttaaac
    11401 tacgttttaa agaaattggc tttattttgc aagcttccaa tctggttcct tttctgacag
    11461 taaaaaagca gttattatta gtagataaga ttaaacatga acatcgtcaa aaagaagcaa
    11521 acgaattatt tgagcagctt ggcgtggata agttgatcaa caagtatcca gaagaactat
    11581 ctggtggaga acggcaaagg gtcgcaatcg ctcgtgcatt gtacaatgat ccgtccatta
    11641 ttctagcaga tgaaccaact gctagtttgg attcagaaaa ggcacgagaa gtagtcgata
    11701 tcttggcaaa agaatcaaaa gacaaaaata aagcaacgat catggttact cacgatacac
    11761 ggctgattga caaatgcgat agaatattta tgatggaaga tggtgtattg aaagaaaaag
    11821 aataaccaaa aagcaggtgg aaaaatggtc catcgataca acaaagatac acagcaaaaa
    11881 attttgaaag aagcgactga cttgtttatg tccaagggat atctcggcac ttcaacaaga
    11941 gaaatcgcac aaaaagttgg gatcacccag cccaatctct atcattattt cggtgataaa
    12001 gaaaaactat atacggcagt tttagaaaac cacttaaaag atgttggaaa agctttgaga
    12061 gaaatcgttc aaacaagcga aacgggattt caagtaacct tgacaaaaat ggcgcagttt
    12121 ttgattgaga cacatctggt cgatttgttt ctgatgcttc acgatcttga aagcaatctt
    12181 tcaaaggaaa caagagatca tcttttcttt ctatggaaga aaaattatcg tgaacctttt
    12241 gaagaaatct tttcagcaaa ccaatctgtg ctaagaaatg ggatttcaaa agaaatcgca
    12301 gcaaggcatt ttttcttagt gttagcacct tacatcactc ggtcgaaaag acctgtggaa
    12361 gaaccgctaa atgtggcgca attgatcgat ttatatttga atggtgtatt aggagaagcg
    12421 taaagctgtt atatagggag aaaatagtta ggtgaagaag caagatatga cgaagatcgt
    12481 tatatcttgc ttctttatat aggtagtttt tcataaagac aaatttgtta ttctctcatg
    12541 attatcaggg tatattttta tttattatgt cggtgttcca ggaagtgttt taatttaata
    12601 acgaagaaat taattgtttg tagaatgaag gaaactattt attgggtaga tgaatcacca
    12661 cgaatagtca aaaggagatt tcaggatatg ccaaaaatga aaaaaatcat tttagcagta
    12721 atgttatttt ctacggttat atcttttaca gccccttttt tgcttactta agctgatttt
    12781 aagttttggc ccttagtctg gatctacgag gtgagagtca agaatgaaaa aaataattgg
    12841 agcttttgta tttgcaatca ttatcattat tatctgtttt caaatagatg tttttggtca
    12901 aaaaaagata gttggacaaa cggtcagcag tacgaacagt acggttacaa agacaaagga
    12961 atcatttgat gatacagaac aagagacatt aatcaaaatc ggaaaaaatg accgtacact
    13021 atatacagat gactcgctaa ctaccccact tgcaactata aacggcggag agctggcgga
    13081 gtttctttct gaaacaaaaa atgcttatca gatcaagaca aatgatggct atactggata
    13141 tttagccctc gaagatggaa ccaaagtcac aaagaatata caaacaaaac cggaagagct
    13201 atctgatgca gtgattgtat tagatcctgg gcatggcgga aatgatactg gggcattaag
    13261 caatgatgaa caaacagaag aaaaagatat aacattaagt acagccataa aagtaaaaaa
    13321 ggcgctagag gatgctggag caacggttta tttgacccat actacagatg aattagtaca
    13381 gcttggagat atatgcgact acagtgaaga aaaaaaggca gatgtcttta tcagtttaca
    13441 cgcagactct acggaatatg cgaatgaagc aactggaatc acgacatatt attactatgg
    13501 acaagaggaa acattagcac agacaattgc tgactccttc actgatctgc ctctggattc
    13561 aagaggaatc tccactggga actatcaagt tcttcgagaa aatcttcaac cttctatttt
    13621 aatcgagatg ggttatatga ataatgatag tgatttaaag gaattagtaa cagacagtta
    13681 tcagcagcag attgctgcaa gtttgacaca agcattgacc acttattttc agtaataaat
    13741 aagcccagcc aaacaaaaat agctcctcat atttttcgtt tggccgggct tattatcgca
    13801 gatgcaagct tttgaacacc gtttattttt ttagtagctt caaagctttt tctgtatttg
    13861 caaagtgaag tttggcatat ttttcaagca taggcatgcc gcctttctcc aatagttttg
    13921 ccgctacttg gtcagatttc gttccagctc ctgatggaag ggtgattcca gcttcagctg
    13981 atgctttgtg taactcttcc aaaaagctgg cacgactgat gatagaagca gcagcaacag
    14041 ctaaatgata ttgttctcct ttagtaacga agtacagttt ttcttgtaat tgattttttt
    14101 ctaaacgcgc atatttttta aaatttgctt ccggcgtaaa ctgatcgatc aaaatcgctt
    14161 ctggtttttc tggctcgatt ttttttagca gcaaatggat cgcttgattg tgcaaagcga
    14221 ctttcatccg aacggcattg tattttggtt ggatctcatt gtatttttct ggagataaga
    14281 caagcagttt ataggggatg gtttttttta agatgtcagc aagctggatg atttggctat
    14341 cttttaactc ttttgaatct ctaacaccaa gtttacgtaa ttctgcaagc tgatcacgtt
    14401 tcacataagc agcacatact gttagcggac cgaaatagct gccatttccg acctcatcac
    14461 tacctaaaac ggataattga ctgatatttt ttggcaaaga agaggctgtt tttgctggct
    14521 tggcttgttt atcctgtgcc tccccccatt tatcagcttc tattgctgca ttcgtacctt
    14581 gaaacatcac ttttccggat gtataagcgg taatggtcgt cccgtctttt tttgcagcaa
    14641 agctagtata aggaatctgt ttatttaaca aataagagcg atagtatgct ttcatttgac
    14701 taagttggtc gtttgttgct ttgataatga cgttgctcat aaaattctcc tcgtttttat
    14761 acataaaact ataccgcaac tcacgacttt tcgttttcta agagattgcg agacatagca
    14821 tttttttaaa gtttatcata taatagttaa gtatgtgaaa aatatttgtg gagaacggtg
    14881 aaagaaccaa cttcatggta cactaaataa aactttcatc aaataggagg aaatgattcg
    14941 tatggcgcac gaaaaaacac gctacaaagc ggtgattgcc aaccaaacat atactattat
    15001 cggacgggaa accaagcatc atatggatat cgtaacgaag ctgatcaatg aacagttagc
    15061 agaattgaaa caattatcac cacaaatgga taatgaacaa gcagcaatct tgatggctgt
    15121 caatgcgctt tcggatcaat tgaagaaaca agaaagaatc ttggagctgg aagaagaaac
    15181 agcagaatta aagaaaaaaa tgatcaaatt tacagagctt gaaaatcgtg tgaaacgcat
    15241 tgaagcaatc gagaacgaag ctcgggaagt attgaaagaa aatggtcaag cagatcatac
    15301 cattaaaaat catgtggaag cacaacagat tttaaacgag aaacgaaaag gacaaattaa
    15361 acaaaaatct tctaattaag atccttttat caaaataaga aaaatttaaa aaaagttaga
    15421 agagaaagga acgtagcatg ctgagtttgc tgattttatt tttattgcta attgcgtttt
    15481 tttctggagc acgtcgcggg tttgctttgc aaatcatatt cgcgattgga tatgtattgt
    15541 catttattgc tgcccagcat ttttacaaac cattagcaag tcatttggaa ttatacattc
    15601 catacccagc tgtcacgcca acatcaaagt tagcattttt tgatcaggta tttgcctttc
    15661 acctagatga agctttttat gcaggaactg cttttctgat tattttgcta attggctggc
    15721 tgcttacccg atttgtgggg gtttttgtcc atggattgac gtatgtgccg atcttgagac
    15781 aggtagactg gattgcagga ggcatcctgt cactaatcat ggcttacgtt actatatttt
    15841 tgattttgca gttacttgcc tttgttccat tagatgtggt ccaaaatcaa ttcggcgagc
    15901 atagtttcgc tcgctttatc gttgagagaa cgccatttct taccaacaag atgtatgagc
    15961 tttgggtaac gaatattttg aattaaggaa aatagtgctg gaaaatattt cgttttttgt
    16021 acagagtaga ttatttaaga aggctgcgta gcgtatgtcc ggcacagctc ttttttcata
    16081 gtgaaagata aggtgataaa atgaataacc gaatccttga aacattggaa tttgaaaaag
    16141 tcaaacagat ggtcagacaa tttgtagtga ctgcacaagg aaaagaagaa ctggcagaat
    16201 tggtaccagt gagcgaggaa caaacgatca caaactggct tcaagaaact gaggatggat
    16261 tgaaagtgca acgtctgcga ggcgggatcc ctatacctaa acttgaaaat atccgtcccc
    16321 atatgaaacg gattgagatc ggagcagact tgaatggagt ggagcttgca caagtttcca
    16381 gagtattgtc tacgacaagc gaattgaatc gtttcattga tgatctttat gacagtgaaa
    16441 tcgaatttgc acgtttgtat atgtgggcag atcagttagt cacgatccct gttttgagcc
    16501 gccgtttaaa ggaagcaatc gatgaagacg gacgcgtgac tgatgacgct tctcctgagt
    16561 taaagagcat aagacagaac attcggcgaa gtgaacaagc tgttcgtgaa cagttagatg
    16621 ggatcgtccg tgggaaaaat gcaaagtatt taagtgatgc gatcatcacc atgcgaaatg
    16681 accgctacgt tatcccagtc aaacaagaat accgcggtgt atttggcggt gtagtccacg
    16741 atcaaagtgc ttcaggacag actttattta ttgaaccaaa acaagtggtg gatttgaata
    16801 atcgtttgcg tcaataccaa atcgctgaac gaaatgagat tcaacgtatt ttaagtgaac
    16861 tttctgctga acttgttccc catcgtcaag aaatcatcca caatgcttat gtgattggta
    16921 aaatggatct gatgaatgca aaagcacgat ttggtaaaga agtaaaagct atcgtgccgg
    16981 gaatcagtga agacaatcat gtcgtcttga aacaagcaag acatccactg atcgatcagg
    17041 aaaaagtagt ttctaatgac atcacaatcg gaaaagacta tcaagccatc gtcattacag
    17101 gacctaacac aggtgggaaa acgatcacgt tgaagacttt aggtctgtta caactgatgg
    17161 ggcaagcagg attaccgatt cctgcaggtg aagaaagtca aatcggtatt tttgaggaag
    17221 tatttgcgga tataggagac gaacagtcga ttgagcaaag tctatcgacc ttttcttctc
    17281 atatgacgaa cactgtagat attttatcaa aagtcaatga aaaaagtctg gtattatttg
    17341 acgagctcgg tgcaggaaca gatccgcaag aaggtgctgc tctagcaatc gcaatcttag
    17401 atgatcttgg taaaaagtct gcttatgtga tggcaacaac gcattatcct gaattgaaag
    17461 tttatggata taatcgggca aatacgatta atgccagcat ggagtttgat gtggatacat
    17521 taagtccaac gtatcgtttg ctgattggcg ttcctggacg aagcaatgcg tttgaaatct
    17581 ctagccgatt aggtcttgat acagaagtta ttgatgaagc gaagcagttg atgaatgatg
    17641 agagccaaga tttaaacgaa atgatcacag atttagagaa tcgccggaaa atggcagaga
    17701 ccgaatatct ggaaatgcgt cattttgttt ctgaggcaca agaacttcac gatgatttga
    17761 aagaagctta cagctatttc tttgaagaac gtgaaaaaga aatggaaaaa gcaaagaaaa
    17821 aagcaaatga agtcgtgtca gaggcggaag aaaaagcaga aaaaatcatt gctgatattc
    17881 gtaaaatgca acagcagatt gggcaaggaa atgtgaaaga gcatcagttg atcgatgcaa
    17941 agacccaatt agcaaatcta catcaagaag aaactttgaa gaaaaataaa gtattgaaga
    18001 aggcgaaaga acaaaaaacg ttgaaaccgg gagacgaagt gttggttacg acttacggcc
    18061 aaagagggac gcttttacgt aaaaacggga atcagtggca agtagaaatc ggtatattaa
    18121 aaatgaatgt gtccgaagac gagctgacgc ctgttgctcc tcaaaaagag ccgacacaac
    18181 gagttatcca tgctgtacga tctgaatcta gtagtcacgt gccgaatcaa ttggaccttc
    18241 gaggaaaacg atacgaagaa gcgctaagtg aggtagatca atatttggat tctgccattc
    18301 tagctggtta tcctcaagtg actattgtcc acggaaaagg aacaggcgca cttcgcaagg
    18361 gaattactga ttatcttaaa aaccatcgga gcgtcaaaag ctttgagttt gcaccggcaa
    18421 accaaggcgg aaatggtgcg acaattgtta aatttaagta aagcttacta aaggtaagca
    18481 gatggttcgc tatccaagta tagaaatgct atactagtca tagattttaa aaatggaggt
    18541 ctttgatatg tcacaagtaa ttacagataa gacatttaat gaagaaacag ataaaggact
    18601 tgtattgatt gatttttggg caacttggtg tggtccatgt cgtatgcaag caccgatttt
    18661 ggatcagttg gaacaagaat atgatgaaga agaattcaga attgctaaaa tggatgtgga
    18721 tgaaaatcca gaaactcctc aacaattcgg tatcatgagc attccaacat taatgttgaa
    18781 aaaagatggt caagtagtag aaaaagctgt tggtgttcat tcaaaagaac aactacgtca
    18841 aatgatcgat caatatttat aaaaacttac agcaaaaata agctgagggt gggactgaat
    18901 ccagttccgt cctcggtttt tttgcggttg agttaaggtt attcaaatta atattttttg
    18961 agtataatta cgttgtcagg ctggattgtt tttttgacag acatgctata ataactttaa
    19021 ttgaatatga aggggagtac cagatgaacg aacgaatcaa aaacaaatta gctttacttc
    19081 ctgatcagcc cggctgttac ttgatgaaag ataaaaatgg aacgatcatt tatgtcggca
    19141 aagccaaaat attaaaaaat cgtgtccgct catattttac tggtagccat aatacgaaaa
    19201 cggaacggct tgttagtgaa atcgaagact ttgaatacat cgttacagaa tcgaatatcg
    19261 aagcattgct cttagagatc aatctgataa agaaaaatga tcctaaatac aacattatgt
    19321 taaaggatga taaaacctat cctttcctta agataacgaa tgaaaaatat ccccgtttgg
    19381 tgattactcg taaggtacta aaagacaaag cactgtattt tggaccatat cccgatgtcg
    19441 gtgcggcgaa tgagacaaag aagatcctcg atcgattatt tcctctgaga aaatgcaaac
    19501 ctagtcagac gaaggagcct tgtctttact atcatttggg acaatgtctg tgtccttatt
    19561 atttcgacgt agatccgaaa gtctatacga aaatcgttga tgaagtgaaa aactttttaa
    19621 acggaggaca cgaaaaaatc gagtcagaga tccatcagaa aatggaaaaa gctgctgaaa
    19681 acatggagtt tgaaaaagct gcagaatacc gagaccagat ccgtgccatt gagacaatca
    19741 tgactcgcca aaaaatgaca aatactgatt tactggatcg agatgtattt ggttatgcga
    19801 ttgataaagg atggatgtgt gtccaagtgt ttttcgtaag acaaggaaag ctaattgaac
    19861 gagatgtttc aatgtttcct ttctataacg aagcagaaga agatttcttg acttacctcg
    19921 gtcagtttta tcaggaaaat gagcatttca ttcctaaaga agtcttgatt cctgatacga
    19981 tcgacaagcc cagtgtagaa gctttgttag ctacgaaagt gctgcaacca aaacgaggag
    20041 aaaagaaaaa attagttgaa ttggcgagta agaatgcctc agttgctttg aatgaacggt
    20101 ttgatctgat tgccagaaaa caggaacgga cgatcggagc agttgaacgt ttaggaaacg
    20161 cgatgaatat cccgacccca atacgtatcg agtcttttga taactctaat atcatgggga
    20221 cgaatcctgt ggctgcgatg gtcgtattca ttgatggaaa accggcaaga aaagaatatc
    20281 ggaaatataa aataaaaaca gtcaacggac cggatgatta tgcatcaatg agagaagtca
    20341 tctatcgcag atattcgcga gtgattcgcg aagggttgcc tttacctgat ttgatcttga
    20401 tcgatggagg aaaaggacaa gtagatgtgg caaaagatgt cttagaaaat caattaggaa
    20461 tcgatattcc tattgctggc atggcaaaaa acgataaaca taaaacaaat gagttgctat
    20521 tcggtacaga tttggctgta gtaccgcttg agcgaaattc accagagttt ttcttactcc
    20581 agcgaatcca agatgaagtc catcgattcg caatcacttt ccatcggcag ctacgcagta
    20641 aaaacagttt tgcttcccga ttagatgaaa ttgaagggtt aggaccaaaa cgaaaaaaaa
    20701 tgctgcttaa agagttcaaa tcgttgaaaa atatcacagc agccacgaca gaagaattac
    20761 aagaaatcgg actgccgaaa aatgtcgcac aaaacgtatt tgataaatta catcaggaat
    20821 gaggaaaggg cactttacaa agaaagtaaa gtgtcctatc attttatgta caacaaaaag
    20881 agcgtttacg ttttgttggt aaataataaa tcgcttacgt ctggtgtcta aggatatatg
    20941 tgatttcttg aagaaggagt gaagaaatgt tagaagtcaa ggatcttgtt aaaacttttg
    21001 ggaactacac agctgtcgat catgtctctt tccaaatccc cgatggaaaa atcatggggc
    21061 taattggcca aaacggggct gggaagacaa cgacttttcg tttgatcttg gattttttga
    21121 cacaagacca aggagaagtg ttatggaatg gccatcatct tggggagaaa gattatgatg
    21181 tcatcggtta tcttccggag gaacggggac tttatccgaa agtaacgatt caagaccaac
    21241 tgctttattt tgcagaactt agagggaaat caagaaaaga gattgaacca aaaatcgact
    21301 tttggatgga aaaatttcag gtaaaaggga aaaagacgga taaagtaaaa tctttgtcta
    21361 aaggaaatca gcaaaaagtc cagctgatcg ccacgttaat ccatgaacct aaattgatca
    21421 ttttggatga gccattcagc gggttagatc cggtaaatgc tgaattgcta aaagatggaa
    21481 tattggaatt gaaaaaacat ggttcatgtg tcattttttc tagccacaat atggataatg
    21541 tagaaaaaat ctgcgatcat ttgatcatgt tgcgaaacgg aaaaatggtt ttgaatggaa
    21601 aagtacatga gattcgtgaa tcatttggcc gtacgaagtt attcttggaa tctggtttaa
    21661 gccaacagga agtagaagaa atcgaaggcg tgaaaaaagt gatccttcat gaagacaata
    21721 cgttagagat taccttagaa gatcctgaag ctgggaaaga aatctttact cgtgcgactc
    21781 agttcggcta cattcctatg tttaaccagc aaccgccaac tttggaagag attttcaaat
    21841 tgaaagcagg tgaaatcgat gcgtaagttt tggattatta ccaaagatgt ctatttaaaa
    21901 aatgttaagt cgatttcttt cttgatcatg atattagtcc cttttatcat gctgggtatc
    21961 atttatgtag caggaaactt tgctcaacaa aatagtgatg tcaataaaat cggcgtattg
    22021 gctgataatg agcaaatcag tcagcaaatg agccaagtca aaacagatga ttttgagttc
    22081 aaagcagttg attctgaaga aaaagcacaa aaagagcttt cggaagaaaa aatcgatgcg
    22141 tatttagtag tctcaactga gaatgataag ataaatggga tgctttatag cgaaaattca
    22201 ttaggacaaa gtacacaact actgatacaa caacaactga ctggcttgca atcgatgctt
    22261 cgagcgagtc aattaggcat ttcaccagaa gaagtagcta gcttgagcca acaagcaagt
    22321 ttgactcgac aaaaagtcaa ttttgattca aaaggaaaaa tgacagtcgg agaagataac
    22381 tcgacagtcc aatacgtcgt aagttatgta gcaacgattg tcttgttcat tattattttg
    22441 acgtatgcgc agatcattgc acaagagatc gcttctgaaa aaggcacgcg gatcatggaa
    22501 gtgatcctgt ccagtacgac tgcacaaaag catttttacg gaaaattaac aggtgtttta
    22561 cttgttgcat taacgcaaat ggcattatac ggtattattt ttggtatagg atacagtcaa
    22621 ttcaaagata tggaaatggt caaaaatata ttagacggta tctcattgga taacttgatt
    22681 ggaccattct tgtggtattc tcttctattc atgatctttg gcattttgat tttttctgtt
    22741 ttagcagctt tatgtggctc tttggtcaac aaagcagaag atacagcaaa agcaattttg
    22801 ccagtgactt atctatctct aggcggatac atgcttggat tgatactagg agcttcagat
    22861 ccaaacaata tcgttattcg gatcacttcg tatattccat tcttatcttc atatattatg
    22921 ccggttcgct tagcaaatga gacagtcggt atgggtggag cattgatttc cttgttggta
    22981 cttgtagccg taacattctt attgatgatc ttgtctgcaa acatgtacaa atcgaatgta
    23041 ctcgtttata gcgaaggcgg attatggagt tcattgaaac aatcgatttc aattatgagg
    23101 aatgaacgaa aaaaagcata aaacaatcct aaaatacaga aggaagctgg acatctattg
    23161 ttcccagctt ctttttgtat agacctattt tatttatttt actatattgg atatatattc
    23221 attgaaaata gtgtataaca actttataat attgagtata ccaatttttg gaggcgtttt
    23281 aatggaccgt tatttagatt tactttcaga aaaattcccc tcaacggagg atgtcatcac
    23341 ggaaatcatc aatctcgaag caattttgca gcttccaaaa ggaacagagt tatttttgag
    23401 tgatatacat ggcgaatttc ctgcttttga tcatattctg cgtatcggtt caggaaatct
    23461 taaagaaaag atcaaagacc ttttttctga acaaatgacg gaaacggata tcagccaatt
    23521 cactattttt acggcttatc cagaatatgc attaacgact gattggtaca aagaacaaga
    23581 taaatcgcag ctgatccatc atttgatcga tcttttacgg tttacgacag tcaaatatac
    23641 ccggtcaaaa gttcgaaaag gattgccgaa agaatatagc tatatcattg aggaactctt
    23701 gtatatcgat gaccgtatca atggaaaaaa agactatgta aaaaaaatca ttgaacaact
    23761 tgttttgatg aaggaagaag aaaaattttt aacgaaactt gcacagacga tccaaaaatc
    23821 tgtcatcgat catttgcata tcgttggaga tatttttgac cgaggaactc aagcagccaa
    23881 agtgatggat cgaatcatgg atttttcttc tgtagatatc caatggggaa atcatgatat
    23941 tttgtggatt ggtgcgtatt gcggctctga ggcatgttta ctgacacttt tacggattgc
    24001 agcacgctat aattatttat ttgaattaga gaaagaatat gggttgaatt tacgtccatt
    24061 gttttctttt gctcatcaaa cttatcagaa gaatccagta tttgcgccta aaaacagtaa
    24121 aaatctatcc gaaagagagc aagaagaatt agaaaaaatc catcaggcac tttctatcct
    24181 tcaattcaaa tcagaacatc agttattgga tcgtcgacct gaatttaaaa tggacgatcg
    24241 taaattgctg gaaaaaatcg actatgaagc atcaacgatt gatttagaag gtcagctcta
    24301 tgagttgaaa ggaacttgtt ttcaaacgat ccagccagat gatccaaaaa aattcttgga
    24361 agaggaacaa aaagtgattg atagtttgat gtattcattc cagcactctt tgagaatgca
    24421 aaaacatatg acgtttttga tcgaaaaagg gggaatgtat ctcacgaaca atcagcatgt
    24481 gctgtttcat ggctgtatac ctgtagacga gaagggacag ctcttagcat ttgaattaga
    24541 ccaatcttat cgcggaaaag aactgctggg cttttttgaa aaacagataa ctgaaagcgc
    24601 aaaagatctg actgcaaaag aagatttagc aaccgatttg atttggtatg cgtggagtgg
    24661 gccattttcg cctttattcg gcaagagtaa aatggcgact tttgaacgtt attttctcac
    24721 agagcctcat acgcacgtcg agaaaagcaa tccttattat cacttgcgag atgaagagtg
    24781 gttcagtgaa aaaattttag cagaattcga cacaaaagaa gaagggtcag caatcattaa
    24841 tgggcacacg ccagtaaaag taaaaaaagg ggagtcaccg atcaaagcaa atggaaaagt
    24901 atttgtcatt gacggaggtt tatccaaagc ttatcaaaaa acgacaggaa ttgccggtta
    24961 ttcactatta tgcaattctt atggtttcca aatcgtgaca cattatccgt ttttgcctat
    25021 tgaacaacaa tttgcaaaaa aatccgacca aacaaatgtg aaaaaagtaa tcgaacagca
    25081 actaccacgc aaactcaatc gtgatacaac aaaaggtatg gaactgcagc agcagattac
    25141 ccagctcaaa aggctgttgg actatcgaaa ggacgattga gaagaattcc agtgaacatt
    25201 gttccataga ctggagactg agacaaaagt cgtgtcgcag tctctttttc cggataaacg
    25261 gtgttcttct gccaactcct cggtcacaag tcacactatt caacaaacat gaaaagctgt
    25321 tttttgaaat tgctccttgt gactcagagt tgaacggcag gctcacgacc tcttggtata
    25381 aacggtgttc aaaagccagt ttctcggtat taagccaaat ttatgcaaaa atgtgaaaag
    25441 ccattttcac aaaatttttc ttaatactcg aaactaaaca gctttttcac aaccccctca
    25501 gaaagtctat taaaattcat tcacaaagaa atcgaataag cgctgttcag atggatgtac
    25561 acggtgggta agttccggat gccattgaac accgaggatt ttatgcatat catctttgct
    25621 ttgaaccgct tcaatgagtc catcgttaga aaaagcgatg gcttttaata aaggtgaaag
    25681 atctttgact gcctgatgat ggtaagaatt cacaaaatat ggatcgggga gaaggttgct
    25741 taacaaactg tctgaaacaa tcttgacttc gtgagtcgta aattgcgggg cagtgggctg
    25801 ttgttcatgt tttactgacc actttggata taatgaaaga tcttgataaa gtgtaccttc
    25861 caaaacgaca ttcaatagtt ggagtccccg gcagactgca aaaattggtt ttctttgttt
    25921 caatgcttcc aagatcagtg cctgttcaaa ttggtctcgt tgaatatttg tttccgttag
    25981 ctttggatga ggatcttcca tgtaaagttg tggactgaca tcttgaccac ctgcaagaat
    26041 aagtttgtcg atttgtccaa tcagttgagg agctgaatct ggagacatga ctggcagaat
    26101 aagaggcaat ccttgagcat ctaaaactgc atccacgaat ccttgcggtg tatagctcac
    26161 ttgatttcct tggaaagtat ctgttgctcg aatcagttga tttccggcaa tgccgatgat
    26221 tggtttcatt tgtattcccc ctaaagtaga cttattgtaa caaggattta aaaaaaatta
    26281 aactatgtaa gacttgcccc tatacttatc tgttcttgct gtgttactat ctgtaaagag
    26341 aaatttcgtg tgaaattcat gacgatagtt gaaaatattg cttaatttgt tataataaaa
    26401 agtaagcaga aataaaaagg ttgggcgtaa gcttcaagga tctagttctt caaggggtga
    26461 gaaggacatt ttattgaagg ggcctgccta tactaagtta tctttagagg tacatacttt
    26521 caatgaaaaa agaaagcatg tacctctttt ttaaatcgaa aggaagtgaa gcaagtgtta
    26581 gaagctttgg aacaaatccg aaaagcagaa gaagaaaatg aaaagcgtgc tcaaacactg
    26641 cggcagcagc tgaagatcta tgcaacagaa aaagagcagg aattggaaac gatccaaaca
    26701 aactatcgac aacaactaaa acagttggtg gaagaaaaag aacaatttga aatacaagaa
    26761 ttagaaaaag aaaaaatgaa attgaaagca gaagcagaca aggtgcaagc aactttgaaa
    26821 gatcaatatc aaaagcatca gagtcaagcg atcggtgcga tcattgaaag ggtgaaagaa
    26881 acttatggcc gtcactaaga tggaaaaagt gaccctcatt tcagataaaa gaaatcagga
    26941 gaggatttta caagcagtac aaggaatcca aaacgttgaa atccgggatc tgtatcaaaa
    27001 tagaacaaac aaccagtggg tggagaagta ttttccagat accacgatta ttgataaaga
    27061 agcaaaatta agcgctttag gtaatcggtt gaaagacatc caagaagcta tccaattcat
    27121 acaacaccac ggcgatagcc gtcaaaagaa actccatttg aaacgtcaag aactttcctt
    27181 acaagatttg gaagccaatt attccgaaga aaatttcata aataaattga aagaagtcct
    27241 tgatttaaaa gaaaagtggg aacaactaac tgaagaacgt gatcgtttag aagaagagga
    27301 agaatggctt cttcattggc aacagctgga tattgctcca agtatgtatg caagtaagac
    27361 gaccattttt gagatgggga ctgtgagtgc agcaaatttt gaaagtttca aggaagaact
    27421 gagtaagctt gatgacgttt atatggaaga aatagattat acgccaaaac aagtttatct
    27481 ttcgtatgtt gctttaaaaa aatcagttgc tgttatagaa gaaattgctg ggaaatacgg
    27541 gttcaataaa gaaacgtatc cttatgaaca aaatcctaaa gaaaaactgc aagagaccaa
    27601 gcagcaatta tccattgttc acgagcagca aaagaagata acgacagcgt taggtcagtg
    27661 cagtggctat atccaagaat ttgaatgggt agaagaagtc acacttgccg ttgcagaacg
    27721 agaaaaaata aaagaacggt ttatccatgc gacttatctg atcgttttgc aaggttgggt
    27781 agatgcagaa gaaaaacaga atttgctgca tgtgttaaac gaaaccgtat cagaagaaga
    27841 tatatacgtc agtttcgaaa caccaaactc agaagagatt cagcaggaag tccctactaa
    27901 attgaaaaac catccgcttg tcgaaccttt tgaattgctg actgaaatgt acagtctgcc
    27961 aaaatatgaa gaagtagatc caacaccttg gatgacacca ttttatcttg tcttcttcgg
    28021 gatgatggtc gcagatgttg gttatggttt gctgatgctc attgggacaa tactagctca
    28081 aaaactactt gttctaccac gcgggatgaa acgtttcgta aaattctttg agattctctc
    28141 gataccatcg atcatctggg gattgatcta cagttcattt ttcggacagt cattaccaaa
    28201 agaactgttt ggcatccgct tgcctttccc aatcctatct acgacggatg acgtcaatac
    28261 gattttgatt ttatccgtga ttttcggtct gatacagatt ttggttggat tatttgtggc
    28321 agcaaaggaa aatatcagga gaaaagatta cttgagtgct gtcagtgatg gatttgcctg
    28381 gcaagggatc ctaattggga tcgttattgc gttggttggt gccatgttga taaaaaatac
    28441 agcatttgtt taccttggcg ctgggctatc gatcattgct gccttatgta tcttgatcgt
    28501 tccgattatc caaacgccgt ctaaagtcaa aggtgctgcc aaaggtgcgt ataatcttta
    28561 cggactgacc ggctatatcg gtgatttagt cagttataca cgtttgatgg cattgggtat
    28621 ttcaggcgga agtattggag cggcattcaa tatgctagtt gctttcatgc cgccagctgc
    28681 tcgattcagt gtagggatcc ttttgatcat cgctttacac gcactgaata tgtttttgac
    28741 actgctaagt gcctatgtcc atggcgcacg attgcagtat gtcgaatttt ttgggaagtt
    28801 ttatacagga ggcggacgtg cctttgatcc gctgaaaacg gcggaaaaat acgtcaacat
    28861 caatcacaag aaaaaaaaca ataaataatt ggaggatttt tatataatga tggattactt
    28921 aattagtcaa aatggtggaa tggtatttgc ggtattagcg atggcaacag cgacgatttt
    28981 ttcagggatc ggttcagcta aaggtgttgg aatgacaggt gaggcagcag cagcattgac
    29041 aacaagtcaa cccgaaaaat tcgggcaggc tctgattctg caattgcttc caggtacgca
    29101 aggtttgtat ggattcgtta ttgccttttt gatttttatc aacttaggaa atgacatgtc
    29161 agttgtacaa ggactgaact ttctaggtgc ttctctgccg attgctttta caggcttgtt
    29221 ttcaggtatt gcccaaggaa aagtcgcagc ggcaggtatt caaattttag cgaaaaaacc
    29281 tgaacatgct acaaaaggaa tcatttttgc tgcgatggtt gaaacatacg cgattttagg
    29341 tttcgttatt tccttcctat tagtgttgaa tgcctaagca taaaggagga aaagcagatg
    29401 aacgccattg aaaaaatcat ctctcagatg aacgaagcag cagagcagga aagagcggcg
    29461 ttagaacaag aagagcgaat gaagatcgat caaaactttg aacaaaaacg tacgcaagtt
    29521 gaaacagaac atcaaaaaca aaaagaaaaa caaatcgaat tactcgaaaa aaaataccgt
    29581 cagctaagga atcgccagca agtagaagta cgacaagaaa atttgaatgc gaagcaggaa
    29641 tttttaagac gattgtttgc agatgcggta actgaaatgg aaaattggga tgaatctgaa
    29701 caaatccagt ttatcaaaaa tgcattgtat tcattacctt tgacaggaaa agtagcattc
    29761 attgctggag aaaaatcagc agcctatctt tctcagacgt tgttggatga atggaacaat
    29821 gaacttccct ttatgatggt tttaagtgat gaaactgttg ctgatcaagc gggttttctg
    29881 ataaacgatc aaggagttca gtataatttt cttttcagca gccttgtaca agatatccaa
    29941 ggaacgatga gttttgagat tgccaatcaa ttattcgaat aaggagggat cagtgtatgg
    30001 aataccacga actaaatccc ttgattcgcg gaagagagct tgaacttttg tccaatgaga
    30061 catttgaacg gatgattcaa acaaattctg tcgaagcact tggtgaaata ttgaaatcca
    30121 cgatttacag tccatatatt tatgacggat atgaatatga cttcgaacag aatcttttaa
    30181 aggagcagag ccgtctattt cgctggttaa aggaatcagc accggagcct gaaatcgttt
    30241 ggatctatac gatgcgttat actttccata atctgaaggt tttgacaaaa gctgagatga
    30301 caaatcaaaa tctggaccat ttgtatatcg acgatggatt ttattcatta gaaaccttga
    30361 aggatgccat tcatacgcaa gtttcaaatg aacttcctga aagcattatg gacagtatac
    30421 gagaagtacg tgattatttt gaagagtcta ccattcttca gggaattgac gtgatttatg
    30481 atcgacagtt tttgacagag cagcgacggc ttgccgataa gctaggatat caagaattgc
    30541 tgacagaagt cattgctttt attgacttga ccaatatcac aacgatggct cgaggaatat
    30601 tgcagaaaag aacatatggg ttcatgacga cggtactttc cagctcggga agtatcgaaa
    30661 aagaagtatt tttgaacttt gtagataaag atatggaaga ttacacgaat tttctattag
    30721 agacagatta tgctgatttt ctgcgccctg tcatccgtga gggaaatatt gatctcgtaa
    30781 agcttgaaca gttgaaagat gattatctaa gttcattgta tcaagaagca caaacccagg
    30841 ctttcggacc actgccacta ctagcctttt tgaacgcaaa agaggtagaa agcaaaaacc
    30901 ttcgtttact ggttatcggc aaaagaagcc atttcgaatc agaaacaatc agaaagcggg
    30961 tgagacaagt ctatgacgca taagatcggt gtagttggcg acaaagattc tgttttgcca
    31021 ttcaaattgt ttggctttga tgttcgttat ggtacaacca agcaaaccgt tcgtcaggca
    31081 attgaagaaa tggcaaagaa tgagtatggc gtgatttatg tcacagagca atgtgcagca
    31141 ttgagcccag aaacgatcga acgttacaag gaacaaatga tacctgctat cgtattgatt
    31201 cccaatcatc aaggatcatt agggataggg ctggaagaaa ttcaaaaaaa cgtagaaaaa
    31261 gcagtaggtc aaaatatctt atagtatgaa aaggagatag aaattttgca aattggaaaa
    31321 atcataaaag tctccggacc tcttgtaatg gctgagaaca tgtctgatgc cagtatccaa
    31381 gacatgtgtc tagtgggaga tctaggagtt atcggagaaa ttatcgagat gcgtggcgat
    31441 gtggcgtcta ttcaagtata tgaagagact tctggaattg gtccaggcga accggttcgc
    31501 tctactggtg aagctttatc tgttgaatta ggaccaggga tcatttcaca aatgtttgac
    31561 ggaatccaac ggccattaga tacctttatg gaaatcacac aaagtaattt cttagggcgc
    31621 ggagttcaat tgcccgcatt ggaccatgag aaaaaatggt ggttcgaacc aacagtagaa
    31681 gccggagaaa cggtgagtgc tggagatgtt atcgggatcg tagaagaaac aaaagttata
    31741 aaacataaaa tcatggtgcc aaatgggata aaaggaacga tcaaaacaat cgaaaaaggt
    31801 tcattcacta ttgatgaaac agtatgtgtc attgaaaccg aaaatggtga caaaaatttg
    31861 acgatgcttc aaaaatggcc agtaagacgt agtcggccaa tcaaagaaaa attaaacccg
    31921 gatgcaccca tgatcactgg acagcgagtg attgatacat tcttcccagt gacaaaagga
    31981 ggagcagcag ctgtacctgg tccttttggt gcagggaaaa cagtcgttca gcatcaaatt
    32041 gcaaaatggg ccgatgtgga catggttgtc tatgtgggat gtggcgagcg tggaaatgag
    32101 atgacggatg ttttaaatga attccctgaa ttgattgacc ctaatacagg agaatcgttg
    32161 atggaacgta cggtattgat tgctaataca tcaaacatgc cagtagcagc tcgagaagct
    32221 tctatctata cgggaatcac aattgcggaa tatttccgtg acatgggtta tgacgttgct
    32281 atcatggcag actccacttc acgatgggca gaagcacttc gtgagatgag cggacgtttg
    32341 gaagaaatgc ctggtgatga aggttatcca gcttatcttg gttcacggct tgcggaatac
    32401 tatgaacgat ctggaagagt cattgcatta ggatcagaag gtcgcgaagg aagtataacc
    32461 gcaatcagtg ctgtatcccc gtctggaggg gatatttcgg aaccagtcac acaaaataca
    32521 ttgcgggtcg taaaagtatt ctggggtctt gattccagtt tagcccaaaa aagacatttt
    32581 ccttcgatcg attggataca aagctattct ctttattcaa cagaagtagg gaagtatatg
    32641 gatcatatcc ttcaagctga ctgggcatcc atggtcaccg agggcatgcg tattttgcag
    32701 gaagaacagc agctgaatga aatcgtgcgt ttggtaggga ttgattccct ttcagataat
    32761 gaccgtctga cattagaagt ggcaaaatcc attcgtgaag attatttgca gcaaaatgct
    32821 tttgatgatg tcgatacatt cacttcaaga gagaaacaat tcaaaatgtt gaagatgatc
    32881 ttgacctttg gtgaagaagg acgccgtgcg ttgtcgttag gtgcttattt aagtgagatc
    32941 atggacggta ccgtatcgat tcgtgaaagg atcggacgaa gcaaatatat cccggaaaca
    33001 gaattagaaa aactagatca aattattggt gaaatcaagc aaacgatgca aacgatcgtt
    33061 tcagaaggag gcatgagcaa tgattaaaga atatcgtacg atcggtgaag tcgtcggtcc
    33121 attgatggcg gttgaaaaag ttgctggtgt caaatatgaa gaattaatcg aagtccgtat
    33181 gcaaaatggc gagattcgtc gtggacaagt attggaagtc caagaagata aagcaatggt
    33241 ccaaatattc gaaggaacaa gtggtatcaa tttacgtgac tcttctgtac gatttttagg
    33301 ccatccttta gaactaggtg tatcggaaga catgatcggt cgagtattcg atggtttggg
    33361 acgaccgaaa gataacggac cagagatcct gcctgaaaag catttggata ttaacggaga
    33421 agtaatcaat ccagtagctc gggattatcc agatgagttt atccaaacag gtatctctgc
    33481 aatcgatcat ttgaatacat tagttcgtgg gcaaaaatta ccagtatttt ctggctctgg
    33541 tcttcctcat aaagaattag ctgctcagat cgcgcgacaa gcaacggtac tcgattcatc
    33601 agatgatttc gccgtagtct ttgctgctat tggaatcact tttgaagaag ctgaattttt
    33661 catggaagat tttcgtcaaa cgggcgcaat cgaccgttct gtcatgttta tgaatttagc
    33721 aaacgaccct gcaatcgaac gtatcgctac tcctcgtatg gctttgactg ctgcagaata
    33781 tcttgcttat gaaaagggaa tgcatgtttt ggttatcatg acagacatga ccaactatgc
    33841 agaggcattg cgagagattt ctgcagcacg gcgagaagtt ccaggaagaa gaggatatcc
    33901 tgggtatctt tacacgaact tagctacttt attcgaacga gcaggaagaa tccgcggact
    33961 aaaaggatcg gttactcaaa ttccgattct aacaatgcca gaagacgata aaactcatcc
    34021 aatccctgac ttgactggtt atattacaga aggacagatc atcttgacta gagagcttta
    34081 caagagtggg attcagccac caattgatgt actcccgtct ctttcgcggt taaaagataa
    34141 gggaactgga cccggaaaga caagagaaga ccatgcagct acgatgaatc agctttttgc
    34201 agcctatgca caaggaaaac aggcaaaaga attagccgta gttttaggtg aatctgcttt
    34261 atctgatgta gacaagatct atgcgaagtt tgctgagcgg tttgaaaaag aatatgtcaa
    34321 tcaaggattc tacacgaacc gttcgattac tgaaacactt gatctagggt gggaactgct
    34381 atcgatgttg ccaagaaccg aattaaaacg aatcaaagac gacatgctgg acaaatatct
    34441 gccagaaggg aagtgagttc tgatggcacg attgaatgtg aatccgaccc gcatggaatt
    34501 gactcgattg aaaaaacagt tgacaactgc cacaagaggg cataaattat taaaagataa
    34561 gcaagatgaa ctgatgcgtc aattcatcct gttgattcga aagaacaacc agctgcgtca
    34621 agaaatggaa gagtctatga cagaagcaat gtctgatttt gtattggcaa atgcttcgat
    34681 cacggaacct ttcatcgaag aattatttgt tttgccggcg gaaaacgttg atttgtctat
    34741 cgtagacaaa aatatcatga gtgtaaaagt acctgtgatg aattttgaat atgatgaaca
    34801 gctgacagac acacctttgg aatacgggta tttgaattcc agtgccgaac tggatcggtc
    34861 aatcgatcgg tttactaccc tgcttccaaa acttttggaa ctgacagaaa tcgaaaaaac
    34921 ttgtcaactg atggcgagtg aaatcgaaaa gacacgtcgg cgagtcaatg cattagaata
    34981 tatgacgatc cctcaattag aagaaacgat ctattatatt cgtatgaagc tggaagaaaa
    35041 cgagcgcgca gaagtaacaa ggttgatcaa agtaaaaaat atgggtacca ctgactaagt
    35101 ggtatctttt tttaaaagag gaaaacgatg attaaacaaa gcaaacaaat aaagaaacgt
    35161 ttgccggctg ttcagcttct cgcattagga ttttttctcc tgattttagt tggcggcggc
    35221 ctacttaccc taccattttt tagtaatagt gggcatggga caaattttat cgatgcgctt
    35281 ttcactgcaa cttctgctgt ctgtgtaacg ggattgacaa cattgaatac agcagagcat
    35341 tggaacagtg ctggtcaatt tttgatcatg cttttgattg agatcggcgg tcttggtttt
    35401 atgatggttc cgattctatg ctttgcattg ataaagaaga aagtcagtct cagcacacgt
    35461 attatattaa aagaagcgct gaatctagaa gaagtatcag gtgtgatgaa gctcatgctt
    35521 tacatcctaa aattggcctt tgtgatccaa ttgattggta cggttgcttt atcttttgta
    35581 tttgtgcctg aatttggctg gttgaaaggt atctggtaca gtttgttcca tgcaatctct
    35641 agtttttgta atgcagggtt tgatttgtta ggagacagtt tagccaatca ccagcaaaat
    35701 gtttatttga tcatggtggt atctgctttg atcatttctg gtgggttagg cttcatcgta
    35761 tggcgtgatt tactggaata tcatcagaaa aagaagatga cgattcattc aaaaatcgca
    35821 ttaaccgtta caggtaccct tttattaggc ggattcgttg tgttcttttt cacagagcgc
    35881 aatggggcaa cgctagtgga agggaatatg tttcaacgat tggcaaacac cttttttatg
    35941 agtgtgacac ctagaacggc tggatattat tcagttgatt acctccatat gagccatgca
    36001 ggtctgattt taacgatgtt tttgatgtac atcggcggaa catctgggtc gacggccggc
    36061 ggattgaaaa caactacgtt aggtgtgttg ctgatccaga tgcgttcgat gttgaaagga
    36121 cgtacgagag cggaagcatt tggccgtacc atccgacaag gcgcagtgtt gcgggcactt
    36181 actttatttt ttgttacgtt gacgttgtgt gtagtcgcca tcatgatttt atcggtgact
    36241 gaaacgatac ctaaaacttc tggcatcgaa tatattgctt ttgaggtctt ctctgcgttt
    36301 ggtacagtag gtcttacgat gggattgacg ccagacttga ctgcaatagg gaaattgatc
    36361 attatagcgc ttatgtacat tggacgagtt ggtgtattga ctgtggtctt ctctctgatg
    36421 acaaaagcac aaagaacaca atcaaaatac aaatatccag aagaaagtgt catgattgga
    36481 taatttcatc ttctgacaga aacctagtga tttagtaaaa taagaaaata aaataatttt
    36541 tcttgcttct tggatacttt aagtgcaaaa tgttcgttta ttttttgagg cactacccgt
    36601 tgttgaacct cgggtagtgc ctctttttta ttgaaaggaa tcaataagat ttgattcctt
    36661 tatttatcaa taggttataa ttcatatttt tttatt
//
LOCUS       DACCYH010000020        35825 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.020, whole genome shotgun sequence.
ACCESSION   DACCYH010000020 DACCYH010000000
VERSION     DACCYH010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.020"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            215..1981
                     /locus_tag="IVY85_000946"
     CDS             215..1981
                     /locus_tag="IVY85_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP8043980.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            1996..2433
                     /locus_tag="IVY85_000947"
     CDS             1996..2433
                     /locus_tag="IVY85_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8043981.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            2446..3597
                     /locus_tag="IVY85_000948"
     CDS             2446..3597
                     /locus_tag="IVY85_000948"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP8043982.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(3659..4357)
                     /locus_tag="IVY85_000949"
     CDS             complement(3659..4357)
                     /locus_tag="IVY85_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP8043983.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(4350..5702)
                     /locus_tag="IVY85_000950"
     CDS             complement(4350..5702)
                     /locus_tag="IVY85_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP8043984.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(5925..7325)
                     /locus_tag="IVY85_000951"
     CDS             complement(5925..7325)
                     /locus_tag="IVY85_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP8043985.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            7486..8223
                     /locus_tag="IVY85_000952"
     CDS             7486..8223
                     /locus_tag="IVY85_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP8043986.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            8348..8827
                     /locus_tag="IVY85_000953"
     CDS             8348..8827
                     /locus_tag="IVY85_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP8043987.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            8824..9591
                     /locus_tag="IVY85_000954"
     CDS             8824..9591
                     /locus_tag="IVY85_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043988.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            9821..10390
                     /gene="lepB"
                     /locus_tag="IVY85_000955"
     CDS             9821..10390
                     /gene="lepB"
                     /locus_tag="IVY85_000955"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8043989.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(10494..11096)
                     /locus_tag="IVY85_000956"
     CDS             complement(10494..11096)
                     /locus_tag="IVY85_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8043990.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            11202..11671
                     /locus_tag="IVY85_000957"
                     /pseudo
     CDS             11202..11671
                     /locus_tag="IVY85_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            11777..12361
                     /locus_tag="IVY85_000958"
     CDS             11777..12361
                     /locus_tag="IVY85_000958"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP8043991.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            12364..13437
                     /gene="prfA"
                     /locus_tag="IVY85_000959"
     CDS             12364..13437
                     /gene="prfA"
                     /locus_tag="IVY85_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP8043992.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            13430..14269
                     /gene="prmC"
                     /locus_tag="IVY85_000960"
     CDS             13430..14269
                     /gene="prmC"
                     /locus_tag="IVY85_000960"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP8043993.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            14262..14441
                     /locus_tag="IVY85_000961"
     CDS             14262..14441
                     /locus_tag="IVY85_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043994.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            14463..15488
                     /locus_tag="IVY85_000962"
     CDS             14463..15488
                     /locus_tag="IVY85_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP8043995.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            15679..16923
                     /locus_tag="IVY85_000963"
     CDS             15679..16923
                     /locus_tag="IVY85_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP8043996.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            17076..18023
                     /locus_tag="IVY85_000964"
     CDS             17076..18023
                     /locus_tag="IVY85_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8043997.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            18157..18786
                     /gene="upp"
                     /locus_tag="IVY85_000965"
     CDS             18157..18786
                     /gene="upp"
                     /locus_tag="IVY85_000965"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP8043998.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(18925..19167)
                     /locus_tag="IVY85_000966"
     CDS             complement(18925..19167)
                     /locus_tag="IVY85_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8043999.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            19288..19360
                     /locus_tag="IVY85_000967"
     tRNA            19288..19360
                     /locus_tag="IVY85_000967"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19321..19323,aa:Thr,seq:cgt)
     gene            complement(19479..20117)
                     /locus_tag="IVY85_000968"
     CDS             complement(19479..20117)
                     /locus_tag="IVY85_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8044000.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(20240..20872)
                     /locus_tag="IVY85_000969"
     CDS             complement(20240..20872)
                     /locus_tag="IVY85_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8044001.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            21223..21522
                     /locus_tag="IVY85_000970"
     CDS             21223..21522
                     /locus_tag="IVY85_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8044002.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            22017..24182
                     /gene="acm"
                     /locus_tag="IVY85_000971"
     CDS             22017..24182
                     /gene="acm"
                     /locus_tag="IVY85_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP8044003.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(24454..24939)
                     /locus_tag="IVY85_000972"
     CDS             complement(24454..24939)
                     /locus_tag="IVY85_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP8044004.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            25419..26060
                     /locus_tag="IVY85_000973"
     CDS             25419..26060
                     /locus_tag="IVY85_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8044005.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            26079..26714
                     /locus_tag="IVY85_000974"
     CDS             26079..26714
                     /locus_tag="IVY85_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8044006.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            26732..27547
                     /locus_tag="IVY85_000975"
     CDS             26732..27547
                     /locus_tag="IVY85_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8044007.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            27866..29302
                     /locus_tag="IVY85_000976"
     CDS             27866..29302
                     /locus_tag="IVY85_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8044008.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            29491..31923
                     /locus_tag="IVY85_000977"
     CDS             29491..31923
                     /locus_tag="IVY85_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP8044009.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            32153..33220
                     /gene="rlmN"
                     /locus_tag="IVY85_000978"
     CDS             32153..33220
                     /gene="rlmN"
                     /locus_tag="IVY85_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP8044010.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(33413..34516)
                     /locus_tag="IVY85_000979"
     CDS             complement(33413..34516)
                     /locus_tag="IVY85_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8044011.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            35068..35616
                     /gene="aac(6')"
                     /locus_tag="IVY85_000980"
     CDS             35068..35616
                     /gene="aac(6')"
                     /locus_tag="IVY85_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP8044012.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT        12277 a         6116 c         7316 g        10116 t
ORIGIN      
        1 ctaaattaaa tgctaacaaa agttattgtt tgcatattat aaaaaaatta aaataacagt
       61 tttatttaaa attgaagttt tctgataccg aaatcttttt attttgctga catactcaaa
      121 cctttggcac aaagtttcgt gccaaagtac gactatttct ctttttagta agcggataca
      181 attagagtat caaaaggaga gagggagaga caaaatggaa cggacgattc ccaaaaacaa
      241 catttcgcta aaagcgaggg tacaaaaatt aggcagtact ttatcaagca tggtcatgcc
      301 aaatattgga gcactgattg cctggggtgt attaacggct ctatttatcc cagatggtta
      361 tttaccaaat gaggctttcg ctaccatggt aagcccaatg ctaacttatc tgattccttt
      421 attgatcggt tatactggcg gaaaagtaat tgctggtgac cgcggatcag ttgttggggc
      481 aattgcaacg atgggtgtga ttgtcggaac agatattcca atgatgctag gcgcgatgat
      541 catgggacct cttggtggat atgccatcaa gaaatttgat cagctattcc aaaaacggat
      601 caaatccggt tttgaaatgc tagtcaataa tttttcagca ggtcttatcg gttttggatt
      661 agcgttatta ggtttttctg cgattggtcc agttgtcgat gcattgactc aagcaatggc
      721 aaaaggtgta gagatcattt tatctgccca tttgattcca ttgacaagta tttttattga
      781 accagcaaaa atcttatttt tgaataatgc aatcaatcat gggattttga caccattggg
      841 aactgaacaa gtagcagagg ctggaaaatc tattctattc ttgcttgaag caaatcctgg
      901 tccaggactt ggcgtattac ttgcttttac tttgtttggc aaaggagcag caaaatcttc
      961 agcaccagga gccatgatca ttcacttttt aggcgggatc catgaaatct attttcctta
     1021 cgttatgatg aaaccgttat tattcttatc agttataggc ggaggaatgt caggaagttt
     1081 cattttccaa ttgatggatg ctggactaag agcgccagct tctccagggt cgatcattgc
     1141 gattctagcg atgacaccgt tcaattctta ccttcctgtt attttgggcg tgattgttgc
     1201 cgcagctgtt tcgtttggta tttctgcagt aattttgaaa gcggatgcga aagatacaaa
     1261 tgaagacttc gaaaaatctg ttcaagagac aaaggcagct aagcaagctt ccaaaggtgc
     1321 ccaaggtatt cctcaaggag ctacattctc tggcgtacaa aaaatcattt ttgcttgtga
     1381 tgcaggaatg ggatcaagtg cgatgggcgc ttcgatcttg agaaaaaaag tacaggaagc
     1441 agacttgcct caaacggtga ccaatcaagc aatcagccaa ctgacagatg actcgaatac
     1501 attgatcgtt actcaggtcg aactacaaga aagagcaaaa caaaaagcac cgagcgcgcg
     1561 ttttgtctca gtagaaaact ttttgaattc acctcgatat gatgaaatca ttcaagaatt
     1621 aagcagcaag cagaaaacat cagtttctga aattgagaat gagccagcag aagagtttct
     1681 ttcagcaaac atcaaacaaa tcaaagagat tctacttgtc cacgatgatc gttccggatc
     1741 agcaacaatg gggatgaccg tgctcaaaga tatattagac aagaaccatt taactatccc
     1801 gttaagaaaa gtcaatatcc aagatttaaa agatgaacct gattcactga tcgtagccaa
     1861 agaaagttta gcggaaaaag tacagatggc tgcacctcaa tccattcatt tatcagtcaa
     1921 cagcatggtc aatccgcaaa aatacgaatc gattgcacaa tatttgaaac agacagctta
     1981 agggaggaaa acagcatgca aagtttgcca ttcgacatga tcgagctcaa tcgatctttt
     2041 aaaaccaaag aagaagcgat ccgctattgc ggtcagatgc ttgtagaagc agggtgcgtt
     2101 gatgaggaat atatcgatgc catggtagat agagacaaaa tgttatctgt ttatatgggg
     2161 aattttatcg cgattcctca tggaacagat gaagcaaaaa aatatgtgaa aaaatccggc
     2221 atctgtgtcg ttcaagtacc agatggtgta gattttggta ccgaagaaga agaaaagatt
     2281 gcgactgttt tgttcggtat tgcaggagtc ggagatgagc atttgcaact tgtacagcaa
     2341 atcgcacttt attgcagtga tatggacaat gttgtccagc tagcagacgc gctcagcaaa
     2401 gaagaagtaa cggaaaatct agcaattgct taaggagaga gaaaaatgag agcagtacat
     2461 tttggtgcag gaaatatcgg tcgaggattt attggagagg ttttggcagc taatggattt
     2521 gagattactt tcgtagatgt gaatcacacg atcattgatg ctttaaatga aagaaaagaa
     2581 tacgaaatcg aattagcaga tgaatcaaaa gaaaaaatca ctgtaaaaaa tgtcaaagga
     2641 atcaacaatc aaacaaatcc agaaaaagta atccaagaaa tcgctgaagc agatttagtt
     2701 actaccgcca ttggcccgaa tatcttacct tttattgctg agttgattgc aaaaggtatc
     2761 caagaaagag aaaaggaagg aagcacaaca tctttagata tcattgcctg tgaaaatatg
     2821 attggcggtt cagcatttct agaaaaagaa gtgtataaat atcttccgga aacatcattt
     2881 actgacaaat atatcggctt tccaaatgca gcagtcgatc gaatcgtccc attacaacac
     2941 catgaagatc ctttattcgt ccaagtggaa cctttcaaag aatgggtcat tgacgatagc
     3001 caaagaaaaa ataaaaaaat ccaattaaaa ggggttttat atgtagatga tctagaacca
     3061 tatatcgaac gcaaactatt tagtgtcaat actggtcatg cgactgtcgc ttatacaggt
     3121 gccttgttag gttaccagac cattgatgaa gcaatgcaag atgcgttagt tgtggcacaa
     3181 ttaaaatcag ttttgcagga aacaggaagt ttgctgattg ccaaatgggg gtttgatgca
     3241 gagcagcatc atgcgtatat tgaaaaaatc attcatcgct tccaaaacaa aaatatctct
     3301 gatgcgatca cacgtgtcgc tcggacacca ttacgtaagt taggttatca agaaagattt
     3361 acacgtccag tgagagaact acaagaacat aacttgactt gcccacattt gacggcaaca
     3421 atggggatta tttttaatta ctatgatcct gaagatgaac aaagccgtca attgcatgaa
     3481 atgaagatcc atgaaaactt ggaacaattg attcaagaag tcacaggaat caatgatccg
     3541 aaaacaatcg ggaatatcaa gcaaaacgtg aaccgttatg ccaagcaagt cgcttaaaat
     3601 aggatacgaa aaaggggaat gtgacgaaat ttttgtcaca ttcccctttt tcgaataact
     3661 aggctgtttg tccttcaaga tagggcagat ccaattcttt accgtaacgt tgttctaaag
     3721 ctaggcggat caaacgagct gccaggttct catttcttgc aaggataggt ccatggaaat
     3781 aagaaccgaa gacatttcgg taaatgacac cttcagattg gtcttcacca ttatttcctt
     3841 gaccttgtac cactttcccc aaaggtcgtt caccttcacc taagaaagtc cgaccattgt
     3901 ggttttcaaa tccataataa gtttcattga attcttcgtt atggataacg atatcaccga
     3961 taaaccggtt attttcctga cttaacgtgt agtgatccag tgcactgatt ccttggatct
     4021 tttctccatt agctcccatg tagtagtgac ctagcaattg gtagccacca caaatggcta
     4081 acatgacgcc atcattttcg atatagttga tcaaagaatc tttctttttc tggatgtctt
     4141 ttgaaatgat taattgctca aagtcttgac caccgccaaa gaaaaccaag tcatatttat
     4201 cagcatcaaa aggttcatgt atactaacga tctccgtatg gcaagaaacc cccatttttt
     4261 ctgctatata tttcaacatc aaaaggtttc cgttatcacc atatgtgttc atcaaatttc
     4321 cataaaggtg ggcgatattg agttcataat tagccacggt tcattcctcc tttgataaat
     4381 ccttgagcag ctagttcttt acgaagctgc aatacagcag tgtaagtagc taaaatatag
     4441 acatgctcag ttggcaattc tttgattttt tcgattactt ctggtaattc ttttgtttct
     4501 gtcaatttat tttctggaat cccggcaact tttagacgta aagtcatatc tgtatgacgg
     4561 tctcctccag aaataacagc tgggatatcc atatcggcaa agttttcaaa attaccatcc
     4621 caaatccagc tgacatcgat tccgtctgcg taattggcat ttaataaagc cactaaagaa
     4681 aatggataag gagctagacc aatcatatcg attacttgat tgatacctac aggatttttg
     4741 acaagtacta atgtacattt tttcccattg atattgatga cttcttgtcg gccgaatact
     4801 ttctcgtcat atcctaaacc ttgtttgatt ttatcaggat cgacattgaa gtactctgcc
     4861 actgcagtag ctgctaaagc attgtagaca ttatacatac cgccaacttc gattccgtat
     4921 tcatgaccat cgatcacgaa atctgctgac acattgtcca tccgtaccat atcagttaat
     4981 cgataatcca attcaggacg tttgaagtca caatttggac agtaatattt ccctaagttt
     5041 gcataagtga tcattttata atgcaagatg tgatgacaat ttggacataa gacaccgtct
     5101 gtattgtaat gagccatttg ttcacgatct tcttcatggt caaagccata atattttcta
     5161 ggattgactg tttcgataga attgaagatt ggtgaatcac cgttactgat aatcgttgca
     5221 tttggtgact ttgcggcacc gtctacaatc attttatagg tcgtatagat ttctccatac
     5281 cgatccattt gatcacggaa aatatttgta aataagaaca gttctggttt catatattct
     5341 gtgactttgc tcaaacttgc ttcatctatt tctaggacag caaacttttt ctgtcctttt
     5401 tttggttttg cctgcaagaa agtcgataca attccctgta ccatattcgc accagtaggg
     5461 ttggttagaa cttcatcgaa ttgctggcgc aaaatattga ctgttaaagc ggtagtcaat
     5521 gttttaccgt ttgttccggt gacaacgacg acttggtagt cttttgccaa actatctagt
     5581 atatgtggat cgactttcag cgctagttta cctggtaaac ttgatccgcc tttaaaaaaa
     5641 gtttttaatg cccattgcga cgtttttcca actgcaatcg caaaatgact gcgtattccc
     5701 attatgtaac ctccaaaaac gatttgctga cgaataaatt caactaatca taccatatgt
     5761 tgaaaaaaag gttaaattgc ttttcttatt cttaagaaaa ggttacggct gaaagtgcat
     5821 gaaatcggac aaaatcgcat gatttaacaa aaaaacagct tgaaacaaaa tgattgtttc
     5881 aagctgctgt gattttatta ttccctctta cctatattcc cattttaacc gataataatt
     5941 ttctcgcttg gatattcata gcctagatct cgtgtttctt ttggcacgaa taacatcaat
     6001 gtatatagca ttccgatccg cccaataaac atcagtgtag caatcgtgat tttgcccaca
     6061 acagacaagt ctccggtaat ccctaaagat aaaccagttg ttccaaacgc agaagttact
     6121 tcgatgatga tcgagatcag cgtttgttct tctgtagcag ataaaaaaac gatacaaaag
     6181 aaacacatac caagagaaag cataaagaca acgactgatt ttcgcacatc atcttgatcg
     6241 attctccgtc caaaaatatt gatgttatct tcacttttga ggaaggaata tagatacaaa
     6301 ccgatgattg ctacagtagt tgttcgaatc ccgccgccta cagaactagg actgcatccg
     6361 ataaacatca gtaaagaaaa aatgatcagt gttgtgattt gaaaatcacc caaatcgtga
     6421 atctgcagac cagcattacg agtcgtaatc gaataaaaca tcgaattgat ccatttgaca
     6481 ctcatattgg aatcttggaa taaatggtcc ttttccaata gataaatcaa gacagttccc
     6541 gaaacaaaca agataacaaa agctaacaca gctaatttgg taaacaagga aaaacgaaac
     6601 ggaagcttcg cattggaacg tttgtataat agccattccc ggcactccat caatacagga
     6661 aaaccaattc ctccgacaaa aatcaaaaac atgatcagga tcaagaaaaa atagtcatgt
     6721 gcaaagggct tgatggaatc cccagtgacg tcaaacccag agttcgtgac agccgatatc
     6781 gcctgataaa aaccgtaaaa aacggcatca cgccaacgat caaaatagcc tctgtaataa
     6841 aaataaatag aaaagaatgt cccaaaaagt aattgaaacc agattaaaat agcaaaagtg
     6901 atccgaatca gtcggacgat accagacaaa cgcggttgat tcatatccgt catgatcagc
     6961 tgtcgctgtt tcagtgtgat ccgtcgttta gaaaaaatga caaaagcagt agaaatcatc
     7021 atgatcccca aaccaccaac ttgaaataat atttccagca gtataatacc gttatcattg
     7081 aacacagaat tgatatcaaa agtcgatagt ccagtcacac tcactgtact gattgccatg
     7141 aacagcatgt ccacaaaaga aacatggcta tctggttcgc gaaaaatcgg catataaaat
     7201 agaacgagtg ataagaccgt catcaatata taataaaaaa cgatgatttg gatcgatgag
     7261 aaatgattgg cagcaaactt ctgtcctcgc cgctgcagcc ttcgaaagaa taaatcaaca
     7321 tgcacagtac tatcctcctg acaatagtcg tttctaccag tatacaaggg gaattaggaa
     7381 tactcaaaga aattatcaaa cagataacaa tacagctttc gattaaaata gaaatggctt
     7441 ataataagga aaatcctaga aaaaagaagg aggaggaata acaaattgat taatgctcaa
     7501 caaatcgtag aaaaaatgaa aaatcaaaaa ataaattatg atacagttct gaaaaaaatg
     7561 atcatgcaat gggagaaaga agaagtaaag cctaagatat taatacatac atgctgtgct
     7621 ccttgcagta cgcacacatt ggaattcatg tctcaatatg cagagatcac gttatttttt
     7681 tcaaattcga atattcatcc taaaagtgag tatttgcgaa gattacatga acaaaaacga
     7741 tttgtagacc aatttaatga gaaaactgac catcatgttg cgctaatcgt tgatgaatac
     7801 cgtccaaatg aattcaccaa aatggtttta acaaaccatc ttgaaaacga aaaagaaggt
     7861 ggtgctcgct gcagtgcatg cttcaatatg cgattagatc ttgttgcgca aaaagcgcaa
     7921 gaactaggat acgattactt tggaagtgcc ctgaccatct cgccaaagaa aaatgcagct
     7981 ttgatcaatc agatcggaat ggatatccaa aagatttacg ggacacaata cttgccaagt
     8041 gatttcaaga aaaacaaagg ctatgaacgc tctttgcaaa tgtgcaaaga gtatgatatt
     8101 tatcgccaat gttattgtgg ttgcgtgttc gctgccaagc agcaaggatg cgatctgaaa
     8161 gaaatcaatc gggaagcaaa agcttactta aaaacatcaa ccgtagtttt tgaggaaaat
     8221 taaagaaatc tttcggaaaa acgtaagaaa aaaataaaga atttatggta gaaataaggt
     8281 agactgtata atttgtccgt agtaaagtaa ggaaacaaga ataaatttca atgaaggaag
     8341 agataagatg cgaaaagtag cacctatttt gaagccaatc gctgtattaa caatctcgac
     8401 gattgtcgcg actactacag gtattgtgaa tagttccagc taccgtgcac caagttcaag
     8461 ttctagtact gtagaagaaa caacaccaac tacttcagtc tcgacatctg aaccaacaac
     8521 aacggaaagt ttgaatgaaa ccactacgac aagtacatgg gacgatacaa caactagttc
     8581 gtcgtatgat gagacatata caactgacag ctcaagcgaa acaagtaata cggtaccaga
     8641 tggcactcca tcggattcaa ccaatccaag tacaagtccg gatacaagtg ctagctccga
     8701 aacgatacca agcagtacga ctgtagatga taacacgcaa aatgatgatg cagcacgtac
     8761 agcacagcaa aaccaagatt caggaaatac ccaaacgtat aaccagcaag caggtgattc
     8821 tcaatgacat ttttactaga ccttatcaac ggggtgcata aattccttgg ctatttagac
     8881 atcagtccta aatatcttaa ccgtggatat acgattttaa gtgtgattcc cacgctttat
     8941 ttattgagaa ttgtttatgg actttggcaa aaccaaaact atcttcaatt tttcttatat
     9001 ggacttgcat ttctagtgct tgtttatttt actgttttaa atgtatttta ttacttttta
     9061 gataaaaaca cgaaagccga tgtcactcag ctttttgtga agtatttacc tgatgaagcg
     9121 ttcaacatcc agacagaaga atcgatagat aatggaaatt cgatcgataa agtgaataca
     9181 caagaggtca tggtttctta tgacgaagat tatcaattga aattagccga gaatatgcgg
     9241 tatttgattg gaaatggtga gatcaaaaca aatgaactcg gtcgcatgga tggatttctg
     9301 gtcgatagaa atacattgta tccttattac tatgtcaaga aaacaggaga gaaagagtat
     9361 gttctaaata ttggacgaag ctactcggac atggaaaaag tagggaccat tcgacttgac
     9421 tcaacagaag acacattgga acctgtcgga ttgttcatcg tcggcggtga ttttgtcaaa
     9481 gacgggttac gctatcatga gccttatcgt ctgaaattga ttgcaaaaaa ggcacagtcg
     9541 caggaagaac cagtggttta tagtcgtagc caaagaagaa gacattcata atgtgatgaa
     9601 acgccgtgta tcagtggttg cgataaaaac gtttattcgg aataaggggg atgtgacacg
     9661 tcttttgtca cgttcctttt ttatataacg agaaagataa gacgaacaca tcacttattc
     9721 tttcctttca tttataaaca ttgtagaata aagaaagtac aacgaagtat tgtgaaaaaa
     9781 cgtaataaat aacgaggaaa atacaagtag gaggtaagct gtgaaagaat ttttgaaaaa
     9841 ttgggggatt ttgatccttg tattagcggc tattttatta gctagagtgt acgtgtttac
     9901 accagttact gtaaacgggc attcgatgga ccctacatta agtgatggac aacgattgat
     9961 ttcatctaag atctcaaact atgaacgaat ggatattatt actacaaagg aaccggggga
    10021 tgaagaacgg atgattgtta aacggatcat cggtatgcct ggagatacag tcaagatgga
    10081 aaatgaccag ttgaccatta atggcaaaaa gtatgatgaa ccttatctgg atgagttcaa
    10141 aaaagaattt tcagaagata agttacaagg agaatatgct tatagctcag gattccaagc
    10201 acaagcagaa agttcatcca cctttacgag tgactttgaa tatactgtgc caaaaggaaa
    10261 gtatcttgtt ctaggcgata accgcttgat ttcaaaagac agccgaatgt ttgggttagt
    10321 cgacaaagat atgatccaag gaaaagttgt tttccgttat tggcctcttt cagaaattaa
    10381 aatcatgtga tgaaaaaata aaaagaagct gtgacatgtt ttttgtcaca gcttcttttt
    10441 ccgaaaattc cttcttattt cttggagttg ctcacttttt ttgcaatctc attttaagct
    10501 ttttggctga tggataagaa aagcgaagcg tttttgttca taaatagctt ttccccacga
    10561 tggaaggtat gactagtagc agagacaaag cccgtttttt ccaatcgatt attcaattct
    10621 tcttggctaa agccattatg gactttcgga tgactaattt gttcattctt gtcaaagtct
    10681 acgatgatca attgaccgcc aggagctaaa atttcataaa gttttgtaag aatattctcc
    10741 gtatcaggaa tgtgtaaaag tacaagtgat agaaggatga gattagcttg gacagcagga
    10801 aattcgacag aagcatcagc gtggatcgtt cggacatttt tcagatctgc tgcttggatc
    10861 ttttcttctg ccattttcag catagtttct gaagcatcgg caatgatcag ttctttgaat
    10921 ctttccgcaa gcggtaaact aacaagccca gttccgcctc cgtaatccag caggactttt
    10981 tggtggctgt cgcgcggtag ataagtagtt agttcttcgc gaataatctt ggctaatgct
    11041 tgacggtcag gggaatcata atggttagcg atggaatcaa aaatattttt tgtcatcaaa
    11101 tagctcctta cgtaaaactt tgaaatttta acttattata gcacgtgaaa agaaagaaag
    11161 catgctatac tcaattcaaa aagggagaat ctagaaagaa tatgccgata tcgtttgtaa
    11221 aagatagaga ggaaaaagga aagtgtgtcc gagaaatatt actagacttg cctgagtggt
    11281 ttgggcttcc tgaatcaacg gaaaaatata tcgaggaatc aagtaaacta cctttatggt
    11341 gtgaaaaaag aaaagaagaa tatttaggct ttatcactct ttcacagaca agtgaagata
    11401 ctgccgaaat ctattctatt gtatgggaat aaaaaaagac tatcatcacc aaggattagg
    11461 aacaaaactt tttaaagaag cagaaggtta cgcagcaaaa cattacaaat atttgcaagt
    11521 gaagactgta gacgaggggc actactctat atatgatcaa actatttgtt tctacgaatc
    11581 attaggattt tcaaggttgg aagtttttcc aaatttatgg gatgaatgga acccatgttt
    11641 agttcttgtc aaaaaattgg aacaaaagtg agcaatagat ttaatactaa tctgtgcttg
    11701 atgaaaattc tcactgtaaa aaatgctgaa aatcaagtat aataccatag gaacaaactg
    11761 gaaggtgaaa aagagcatgg cacaactatt ttttaagtat ggcgcaatga atagcggcaa
    11821 gacgatcgag atcctaaaag tcgcacataa ttatgaagaa caaaataagc cagttgtctt
    11881 aatgacaagc ggtattgata caagagatgg tttaggcgtt gtttcaagcc gaatcggact
    11941 gaaacgtaat gcgatgccga tatttgaaga aacagacgta tttgacgtaa ttcagaaaat
    12001 ggaaagcaaa ccctattgtg tgctgatcga tgaagcgcaa ttcttaaaaa agaaacatgt
    12061 cattgctttt acgagaattg tggacgaatt ggatattccg gttatggcat ttggtttgaa
    12121 aaatgatttt cgaaacgagt tatttgaagg atcaaaatat ttattgcttt atgcagacaa
    12181 aatcgaagaa atgaaaacaa tctgttggtt ctgccacaaa aaagcaatca tgaatttaca
    12241 ttatattgat ggaaaccctg tttatgaagg cgatcaagtg caaattggcg gaaatgaagc
    12301 ttattacccc gtttgtcgaa aacattactt tcacccatca atgattacag aaggagaata
    12361 agcatgtacg atcaattaca agcaattgaa gaccgttatg aagagcttgg agaactttta
    12421 agtgacccag aagtgatttc tgatacaaag cgttttatgc agctttcaaa agaagaagca
    12481 aacacgcgcg aaacagtcga agtttatcgt cgttataaag aagtagtaca aggaatcacc
    12541 gatacagaag aactgcttgg tgaaaaactg gatgatgaaa tggctgaact agcaaaagaa
    12601 gaattatctg aattaaaaaa agaaaaagaa gtcttagaag aaaaaatcaa gatcttgctt
    12661 ttaccaaaag atccaaatga cgataaaaat atcatcatgg aaatccgtgg agcagcaggc
    12721 ggagacgaag cagcattatt cgctggcgat cttttcaata tgtaccaaaa gtatgctgaa
    12781 gcacaaggct ggaaaacaga agtgatggaa gctaacgtta ccggaatcgg tggatataaa
    12841 gaagtgatca tgatgatcac cggagacaac gtatattcga aattaaaata tgaaagtggt
    12901 gcgcaccgcg tccagcgtgt tccttcaact gaatcgcaag ggcgtatcca tacttccacc
    12961 gcaacagttg tcgtgatgcc agaagctgaa gaagtagaga tcgatattga agacaaagat
    13021 atccgtacag atatctatca tgcttcaggt gctggcggac agcacgtcaa taaaacagca
    13081 tctgctgtac gtttgactca cttaccaaca gggatcgttg ttgctatgca agatgaacgt
    13141 tctcaaatca agaaccgtga aaaagcaatg aaaatcttgc gtgctcgtgt gtacgataag
    13201 atccagcaag aagcacaaag cgagtatgat gccaatcgaa aatcagcagt cggaactggg
    13261 gatcgttcag aacggatccg gacgtataat ttcccgcaaa atcgtgtgac tgatcatcgt
    13321 atcggattaa ctatccaaaa attagatcag attttagcag ggaaattaga tgagatcgtt
    13381 gatgcattga tcatgtatga tcaaacatca aaactggaag ctatgcaaaa tgggtaaaat
    13441 gacctatcga gaagtcctgt cacgggcttc ttctttttta gaggatcatg ggaaagaagc
    13501 atatagtatc caatttttat ttttggaacg aaaacaatgg aaaaaacttg attggctcct
    13561 tcatatgaac gaagagattt cagaagaaga acagcggttg atcgaaaccg atctgcgact
    13621 tctgcttgca gatcatccac ctcagtattt gctaggatat gcagattttt atgatcatcg
    13681 gttaaaggta actgaagcaa cactgatccc tcgaccagaa acagaggagt tagtcgaatg
    13741 gtgtttagac gagacgcctg gtgtaccgtt agaagtgata gatattggga cagggactgg
    13801 agcaattgct attagtttga aagcagcaag aaaaaattgg catatatctg ctgtggatct
    13861 ttcagaagaa gcattagaag tagcgaaaga aaatgctcag aaagaaggaa cgaaaatcag
    13921 tttttatcat ggagatacat taggaccagt aatggatcag caatttgatg tgattatttc
    13981 taatccgccg tacatcagtc ggaatgaatg ggaactgatg gatgaaagtg tccggtcatt
    14041 cgaacctaaa atggcccttt ttgctgagaa cgatggatta gctatttatg aaaaaatagc
    14101 agttgaagca ccaagcgtac taaaatcaaa tggaaagatt tttttagaaa tcggatttcg
    14161 ccaaggtgaa gcagtaaaaa aaatatttca gcaagcattt ccagataaaa aagtggcgat
    14221 aaaaaaggat ctttttggca atgaacgaat gatccaagtt tatggttgaa gttgtggatg
    14281 aaaaattttt tatttgtttt tttattataa aaaaacactt atttgacaag gatgtaaacg
    14341 actttttggt ttatcaacaa gtttatccac tattgacaaa tagattatcc acaaacagtg
    14401 gaaaacttga ggataaactc agacaaaata agaatgtatg aaaaaggggt tgtggataaa
    14461 ctgtggaaac gaaacgatat gatgaaacag aattgcaaga ggcagccgaa gcactgaaag
    14521 aaggagaact tgttgctttt cctaccgaaa cagtatacgg attgggcgcc aacgctcttt
    14581 taccaaacgc tgtcaagaaa gtgttttctg taaaagggag accgcaagat aatccattaa
    14641 tcgtccatgt cgcctctttt gaacaagtaa aagagtatgt ggacaacttt catccggcag
    14701 cagagaagat tgtgaagaac ttctggcctg gaccattaac tttgatattt aagatcaaaa
    14761 aagacacttt gccctcagtt gtgacgggag gattatccac agctgcattt cgtataccgg
    14821 ataacaaaaa aacactagaa gtaatcgaac tttccggtgt tccgcttgtc ggtccaagtg
    14881 caaatacgtc gggaaaacca agtccaacga ctgcagatca tgtatatcat gatctacaag
    14941 gaaaaattac cgggattatt gatgatggag ctacgcgaat tggtgtagaa tccactgttc
    15001 ttgatttaag tgatccaaca gccatgccta tgattttacg accaggtgca gtaacgaaag
    15061 aacaaatcga agcagtcatc gaatctcctg ttgctattga tcagcatctt gtaaaagaaa
    15121 atgaaacacc aaaagcccct gggatgaaat acaaacacta ctcacccgat acacgtgttc
    15181 tgatggtcag agatggcgat tggtcaactg cagttcaatg ggcaaaaaat aagaagatcc
    15241 gtgcaggtgt catagcaagc cccgaaattg cggatcaagt gcggacagat actgcagctg
    15301 tttatatgta taacgacaac tcagtagaag cggcggctaa aggattgttt gcaggtctaa
    15361 gaggattaga tgaaccaaca cttggattag atttgatttt tgtacaagtg tatccagaaa
    15421 ctggattggg gaatgcttac atgaatcgct tgaaaaaagc agcaggacaa aattattttg
    15481 aaaaatgatc aattggttat tgtcgcattc ttttagtatg atacaatgct atcaagtaaa
    15541 tcttctatca gtgaacgaaa aactaagcgt aactaatcgt aatagaagag atacgaatat
    15601 ttggcaattt gtttttgatg ggaaaaataa aattgtagga aaataaaatt gtaggaaaat
    15661 aaaaaggagc tgttgtttgt ggtagattac aaaacgtttg accctgatct ttgggctgcg
    15721 atcgccaaag aagaagaaag acaagaacat aatttggagc tgattgcctc tgaaaacttt
    15781 gtctccgaag cagtgatggc tgcacaagga agtatcttga caaataaata cgcagaaggt
    15841 tatccagggc atcgctatta tggtgggtgt gaatttgtcg atatcgttga aaatttagcg
    15901 attgaccgtg caaaagaatt attcggagcg aaatttgcga acgtccagcc tcactccggc
    15961 tctcaagcca acacggctgc ttatctcgct ttggtagaac caggggacac gatcttagga
    16021 atggatcttt ccgcaggcgg acatctaaca catggatcac cagtcaactt cagtggcaaa
    16081 acctatcact ttgttgctta cggtgttgat ccgacaacag aagttattga ttataatgtg
    16141 gtacgtattt tagctagaaa acatcaacca aaattgatcg tagcaggtgc cagtgcttac
    16201 ggacgaacga tcgactttgc gaaattcaga gaaatcgcag atgaagtagg cgcaaaactg
    16261 atggtagata tggcacatat cgcaggatta gtagcagcag gacttcatcc taatccagtt
    16321 ccttatgcag atatcacaac gactaccact cataaaacat tgcgtggccc tcgaggcggc
    16381 atgattttga caaacgatga agcattagca aaaaaaataa atagtgcggt atttcctggt
    16441 attcaaggcg gccctctaga gcatgtgatt gcgggtaaag cagtagcatt caaagaagca
    16501 ttagatcctg catttaaaga atatagtgaa caaatcatcg ccaatgcaaa agcaatggtc
    16561 aaagttttca atcaagcaat tggaaccaga gtcatttcag gggcgacaga taatcatctg
    16621 atgttgatcg atgttcgcga gttaggtata aatggaaaag aagcagagag cattttggac
    16681 agtgtgaata tcactgtaaa taaaaactcg attccttttg aaacactaag tccattcaaa
    16741 acaagcggta ttcgaatcgg cacgccagca atcactactc gcgggttcaa agaagaagat
    16801 gctgtaaaag tggctgaact agttgtcaaa gcattacaag caaaagatga taacgctcaa
    16861 ctagacgaag tcaaaacagg tgtacgcgaa ttaactgaaa aatttccgtt gcataaaaaa
    16921 taaaaattta aattccatta gtaaacagaa aagttcaatg ttgggctttt ctgtttattt
    16981 tttgggaaaa cggattcttt gacaatatcg gtgatgctct ctagtatatg aggtgtaatt
    17041 ttagtcttca cactaaatct cagggaggaa aagtcatgac tgatcgtatc tcgttagaaa
    17101 aggaagcagt agcattcagt gatgcaaatg cgcctcatcc gcgtatctat gaactagcac
    17161 cggaagaagg aagagtttta ctagaagaag tacaagaatc acctgtgaac aaatattccg
    17221 tcgatattga agacttgatg gtggatactg gcgaatgggg aaaactcaat attcgtctcg
    17281 tacgaccaga agggaatatg gataaattac cagttatttt ttatattcat ggagcaggct
    17341 gggtttttgg cagtgctcaa acccatgata aattgatcag agaacttgcg gttcgtacga
    17401 attccgtcgt tgtctttcca gaatatagtc gatcaccaga agcaaaatat ccaacagcaa
    17461 ttgaacaaaa ttatgctgtg ttgcaacagc tgaataaatt agccaagtca aaaaatctgg
    17521 atctcgagaa tctaatcgtt gcaggagatt cagtaggtgg taacatggct acagtcatga
    17581 cgatcttaac aaaagaacga aagggcctcc caatatccaa gcaattgctg tattatccag
    17641 tcacaaacgc tgattttgat acagattcct atcatgaatt cggagaaaac tattttttga
    17701 caagagaagg aatgatgtgg ttttgggacc aatatacgac gaatgaagaa gagcgtgctg
    17761 aaatcacagc ttcgcctttg agagcaaata taaatgaact aaatgatctt ccttcagcaa
    17821 tgattttaac gggagaagcg gatgtattaa gagatgaagg tgaggcttat gcgcgcaaac
    17881 ttcgtgatgc aggggttcct gttacacaag tacgcttcca aggaatcatc catgattttg
    17941 tcatggtaaa tgctatggat cagacaaagg caacgcgtgc agcaatggca ttatctactt
    18001 cttggatcaa gaaaaaagaa taagctttgc ttgtccgcag gaaaacttct tttcaaaaac
    18061 ggattttctt aggaaaaatg gcgagtttga ctagtcaatc acttgatttt gttttacaat
    18121 taaaaagaaa cgacagaaca aaggagagct taaaaaatgg gcaaatttca agtgatcgat
    18181 catccattga ttcaacacaa actaactatt attcgggaca aaaactgcgg aacaaaggtt
    18241 tttcgtgaag tagtcgatga aatcgctatg ttgatggcat atgaagtatc acgggatatg
    18301 ccattggaag acatcgtgat cgaaacaccg atcactgaaa caacacaaaa aacgttgtct
    18361 ggtaaaaaag tagcgattat tccaatcttg cgcgcaggta ttggaatggt tgacggtatc
    18421 ttggaattga ttccagctgc taaagtggga catatcggtc tttatcgaga tgaagaaaca
    18481 ttggaaccac atgaatattt cgtcaaactt ccagaagaca ttgatgcacg tcagttgttt
    18541 gtagtagatc caatgttggc aacaggcgga tcagcaatca tggcaatcga tgcattgaaa
    18601 gaacgtggcg cgtcgaacat taaatttgtt tgtttggtag cagcacctga gggagtaaaa
    18661 gctttgcaag aagcgcatcc tgatattgac atctatacag cttctctaga tgaaaaattg
    18721 aatgaacatg gttatatcgt tcctggacta ggggatgcgg gtgaccgttt gtttggtaca
    18781 aaataagtaa atacgaaaaa gggtgtgaca caacttttgt cacatccttt tttggtcatt
    18841 tttaacgttt gatgcagaac agctttttca cagctttcgt ttgcatataa aaaaaccatc
    18901 ccaaaaatgc agagatggtc aaattcaaat cttttttagt tctttgtagt taacgattac
    18961 tacaccctta ctttgcgtca tcaagttcgc attttctgca gtttcatcaa tctcaacaac
    19021 agcggaattt ttcaattgct ttgaaatcgt tccagtttgc gacttgccgt gaattgtgaa
    19081 cattacgtga gtattgatct tgaatttttc accaatttcc gatggtacga tagttgtatc
    19141 aaattttcca aaagttgcca ttttcataaa acctgccttt cacatcaatt ataccacgtt
    19201 ttatgaaaaa tatcatgaaa ttaaactaat tttccatcgg actctttaca atcagccaaa
    19261 tagagtatat aatttctttt ggataaggcc agcttagctc agttggtaga gcaacgcact
    19321 cgtaacgcgt aggtcgtagg ttcgatccct atagctggca ttaaaagaga agacagatat
    19381 atcaaggatt tttaatccat tgatacatct gtctttttgt ttaaaggaaa taaagtatgc
    19441 tttcttagca atagagcatg tattatttct ataaaaattt aggagaataa aacgactagc
    19501 aatatagctg tgaaatcaaa tatcacatga actgcccaac ttgtaatgat cgagttgctt
    19561 ttaatggcag cttggttgaa taacaatctt gccacccctt gtatcaaaag gatgtgtgct
    19621 acagtgtgta aaatatttcc attatcataa gtactgtaat ggataaatcc aaagatgatt
    19681 gctgacaaga aactagcgac aggaatgctg aacttactag aaaaaatagc gagaaaatac
    19741 actgagaaca attcttctcc aagtatcatg ataggcaaaa taataaataa taacgggctg
    19801 aaagcctcat ttattggatt gcctgttaaa ccccatttca aaatttgttg caaaaatacc
    19861 gagactgcgg cactgattac tacattgata aaaaaatatt tggctatatt tttgacagga
    19921 gcgacaggtt tcgagaacat ttttttcgta tcttttttac caaaaacaag tgtaaatgct
    19981 ataaatccag tgatagttaa agaaaaaatg ctaataatgg atggcgcaaa tagcaaccct
    20041 aacaaaccga gtacaacgac aatagtgccc aatatccctt ttttaagttc tatttttttt
    20101 cctaaaatgt tattcatgat aataactcct actattcagt ttttgaagat agagtcagta
    20161 tacatgaaga gttaaaatag gcaatgtttt tttatggata tttgtattgc taaaaattta
    20221 tcttatatat tcattttgat tatactactt ttatttgtaa aaattttcta gcaaaaccaa
    20281 gtgcttgcaa ctgattaagc gtacaatttt ttagatgttc cgagcgtact tcaatcattt
    20341 caaaggagtt gctacttaaa tcgatatttt ttaaatcagt atcaaaaaag ctagcatttt
    20401 ctaaatcatt agtagaaaac attattttag tgagggaaca gtctacaaaa tctgcgcttg
    20461 ataagacaga gtgctcaaag gtactttctt ttatgtttga accagataaa tttacatagt
    20521 ccattttaca attctcgaat aacgtgttcc caatataact ttccatcaat ttagtacctg
    20581 ttaatttaca attgacaaat ttcacgcgat gcaacatgct ttttgtcaat atacagcctg
    20641 aaaaatcaca gttttcgaat atacagtcgg taatctccat tctttgaaaa tcattggaag
    20701 caaaaaatgc atacttgaaa atagatttgt aaataattgg atggcagatt atttggtttt
    20761 cgatgacctc tttttgtgaa aggatattat acagttcaaa atcttcgttt tcatagatat
    20821 gaaaaaagga tcctttattc aagcttgctt cattaacttt aggaaaaatc atatttaatt
    20881 acctccattt tcataaagtc atattaacaa aagctaaacc aacttacatt agcagaaaat
    20941 gagataggtt ttcgcctttt tcgataacga tagtgtcgct atctcttgaa acgttcttag
    21001 aattgccctg agagacacaa attaggcgta attctaataa aaatcttaga ataaaaattt
    21061 ttactgattt aatttagaat gtttttaaaa taaaaatcgt tgccataaca gtgtattgaa
    21121 aggttttaac aatccagtaa tgatttaaaa aaatctagat gtagcgtata ctttgtttat
    21181 agaatgtagt gggaagaaat atatttttgg gaggaaatcg tgatgtctaa aagtgaagaa
    21241 atagaaaaca tggtactaaa attatatgat ctcgttttaa atccagagat caaagaaaag
    21301 gaacgtatac ttttgataga cgcaaagaca ggcttagaaa aaggtcagta ttatcctaaa
    21361 gtcataaata atttggaacg tagtttacgt ccgttagcaa tcaggggaga attgtctaaa
    21421 ccagtatcac cgttttatat ggagatatca acaataggaa aatttgaaaa agaattaggt
    21481 cgaggaatgg cttcggcacc gattactttt gggcatttat aaaaacgagt tattcatctc
    21541 aatgaattaa aaagtatagt caatctcata atgcaggcta ggttcattta tttgaacaga
    21601 gtctgtattt ttttattcta aataaagaaa cagatgtttc tttatattgt attttatcct
    21661 ttaattaaca caaaaacagc tagtataaac taataatgtt aattaaaagt gtattttatg
    21721 aaactatcgt tatttattaa tgttgcattt ttgtaagtta aaaattggaa atatagattg
    21781 aaaaaatgaa agcaataagt aaaataaaat gaatatatgc gaaaaaataa tttacattag
    21841 gaaaaacctt gtaaaaacag ttttttggca tatttatttt ttataaatat ttgtaaaata
    21901 actatcaatt ttttagttaa aagattggaa atgagtgggg gaggaattga agaaaggatt
    21961 tttgagagat gatatagtag gttttatttt tagttaaaat aggggagata gaaaaaatga
    22021 aaaagtgtag aaatattatt ttatcaatgt tatttattat tactaatata acatcactga
    22081 ttcctgttca tgtctatgcg gatgcaggca gagatatcag cagtaatgtc acttcgttga
    22141 ccgtggaccc aacgaatatt acagatggag gaaacataaa agtaaaattc tcgtttgacg
    22201 aaaaaaaaca aaatatccaa ccaggtgatt atctttggat caattggcca agtgaaggaa
    22261 atattcgcgg tgaaggattc caaaaagaaa tcccattaat gattgaaaat aaaaatgttg
    22321 gaactttaac ggtcagaaaa gattctgcgc aggttgtgtt taatgaaaat attaaaaacc
    22381 tagactcagt agaaggttgg gggcaatttg agatacaggc cagaaacgta accgataccg
    22441 gtgaagaaaa tacgggacca ttcaccgtga cgagcggtga taaaacagct actgtaaatg
    22501 tgacaaagcc agcttctggt tcttcttcta gcgtttttta ttataaaaca ggagacatgc
    22561 tacctgaaga tactaagcac attcgatggt ttttgaatat taacaataac ggcacttatg
    22621 ttgaacaacc agttaaaatc agtgatgaaa tccaaagtgg acaaagatta gatccaagta
    22681 cgtttgaaat taatcaaata cacttaggtg aacagaaggt ttatagagga gaagagggaa
    22741 tccaacaatt tttacaagat ttccccagcg ctacatttaa tttttcagta actgataatt
    22801 acattgaaat aactatacca aaaaactttg tcaatcttag gaaaataatg gtgagttata
    22861 agacgatcat agaaaaccca gaacaaataa attttgagaa tcattcggaa gcttggttta
    22921 aagaatttaa taaacctgct gttgatggag aaagtttcaa tcatacagtt aaaaatattt
    22981 cagcttctgg tggagtcaac ggtacagtta gaggcgaatt gaagattttc aaatatatca
    23041 atgacacaga gattggtatc ccaaatgtga ctttcgaatt aagacgagca gatgaacagc
    23101 ctattcaagg acagtctagt atcctgttga cttcgaatga acagggagaa gctagtatca
    23161 aaggcctgca agtaggagac tatgttgtaa aagaaaaaga agcacccaat tggattgatt
    23221 ttgatccact aagttcaaat gaactcaaat tctccataaa cgaaaatgat acagagggag
    23281 tcagtttacc tatttataac aagaaaaagg ttacgaacat cacagcaact aaaatttgga
    23341 acggtggaac cactccaaga ccgagtattt attttaaatt attcagagca agtacaaaca
    23401 attgggaacc agttcctgat gcagaaacaa aacgattaga caatggaata acttcagtga
    23461 cttgggaaga tatccaacag tatgatgata gtggaaacga atatacgttt aaagtgcaag
    23521 aagtagatca aaacggaaat gattatgtcc cttcaggtta tcaaaaaatt gaaaacgggc
    23581 tagtcgttac aaatgagaat aaagaagtca tttcagtctc aggacaaaaa acatgggaag
    23641 acaatgagaa ccaagacggc aaacgaccga cgacgatcac agtaaatcta ttagccgacg
    23701 gtcaaccgat ccaacacaaa gaagtaagtg aaaaagatga ttggcgctat cgtttcacaa
    23761 atctgccaaa atacagagac gggcaagaaa tcatctatac tgtaacggaa gacaacgtgc
    23821 ccgaatacag tacgacgatt gacgggtata acatcaaaaa cagttataca ccggaaaaaa
    23881 caaatatatc tataccaggt agccatataa caccgtggaa aaattttcca gagaagacag
    23941 agagagagaa aattagaaat ctattctctc aaaaattgca aatatttgct catacaggag
    24001 aatccagcga acaaagaagc aattatatta gtaagagagc tgtacctaaa cagataacaa
    24061 ataaatcaaa aagcattctt cctaaaacaa gtagtaaaaa atctagtttt gctgtaatca
    24121 ttggtttatt gatagtaact atctgtgctg ggatattctt acaaaaatat aaaaaagttt
    24181 agttagaata taatattttt tgttaaatat gactgaaatc aaaagaataa aattccgatt
    24241 tataatcgaa aaaagaagca tttttatata tgcttctttt ttcgtagaat caataaatat
    24301 ggatttggct aactatggaa gaatactcgt attagttttc agcgttcata ctagttgtta
    24361 cattgtttga ttggccaaaa gaatttctga tatattttgt gctaaataaa gaaaaagacg
    24421 cttgaattgg gaaataagaa aactactcta tcattacaaa ttttcataaa ctggcagatg
    24481 ttctaagtaa acaagtatct tttcaaagtc ggaaaaatcc ataatcaacg tagtggtatt
    24541 gatatttgga tgaaagccta cggtatcttg acgatcaata gcagcatcga tcacgacttg
    24601 gatttttttc tctttgtcgt gcatcaatgc taatggagtc actgtacctg ctggaacgcc
    24661 taggaggtca ggaagctgtt cttcagaaac aaaagataag cgcttttctt ttaattgttc
    24721 agctagttgt tttaaatcgg cgtgcttttc atcaggcaag atgacaagat agatttgttt
    24781 tccttttctt gatttcaata atagattttt tacttgcgga ccaggtaggg aaaaagggac
    24841 atttttgact gacgtaatcg caggatgatc gactttttga aaagagatac ccagtttttt
    24901 tagtagttca taagcttctt gttcggttgc acgactcatc gcgcatcacc ctcctttttt
    24961 tattagtgta tcataaaaaa gagttgacaa atgaaaaata atcatgtaaa ttacacgtaa
    25021 agttaaaaca aatgacatcg aaaagaagag tagccgttag gtgtttttag agagtctctg
    25081 gttggtggaa agaggcagca gttaaccgtg aaacacatct tggagtcgat tttctgaagc
    25141 gtatagtagg aaaatccggg gagcccgtta cagctgcggt cgttgctaga tgaccagata
    25201 aggctggctt ttccgtgagg agtcggtaaa aaaaggtgga accacgtgat taacgtcctt
    25261 tgatcttcgg atcaaaggac tttttgtttt catctgaacg gcaacgactg tgtgaggttt
    25321 tttctgcaag gaaaaaataa acagaggtgg aaccacgtat aatacgtcct cttggcattt
    25381 gccaagagga tttttttatt ttaaaggagg aaaagaagat ggagtatttg attgaagtta
    25441 tgccgcaatt attagtagga gcactcacaa cattgaaatt atttttattt accttgattg
    25501 gctcattacc attagggatt gttttagcat taggattgtc tttgcatttt aagccgctac
    25561 gctacttgct tcaaacatat gtatgggtta tgcggggaac accgctgttg ttgcaactga
    25621 tttttatttt ttatgggttg ccgacgattg ggatcgtttt tgatcgtttc gaagcagcac
    25681 tcattgcctt tatcttaaac tatgcagctt attttgcaga gatctttcgt ggagggatac
    25741 aatcgattcc tcaaggtcaa tatgaagcag cgcaagtttt gcaattaagt ccctttcaaa
    25801 cggtgacgaa aatcgtcatc ccacaagtga caaagattgt tttaccttct gtagggaatg
    25861 aagttatcaa cttggtcaaa gattcttcct tgatctatat attaggttta ggtgacgtaa
    25921 tgcgtgctgg gaaaatcgcg atggaacgcg acgcgacttt actgcctttg ctaggcgtgg
    25981 caatcatcta tcttacattg actggtattc taacaatcgt aatgaaacaa gttgaaggaa
    26041 aattaaatta ttaccgctaa ctgataggag gaagaaatat gattgaattg aagaaagtga
    26101 acaaagcatt tggtgaaaaa aaaataatca aagatttgaa tctaatcatt ccagatggga
    26161 aaacattagc tatcgtaggt ccttccggtg gcggtaaaac cacgctttta cgtatcttgg
    26221 ctggcttaga aagttctgac agcggagaat tttatttaga cggtgtaccg ttcaacccat
    26281 tcgagcttaa aatgaaagaa caagttgtag gagtcgtatt ccaagacttc cagctatttc
    26341 cacatctgaa tatttttgaa aacattacct tagcaccaaa aatggtattg aaacagacaa
    26401 aagaagaata caccaaaaaa gcagaagaat tagcggcttc attagggatt agtgatttat
    26461 tgaatatcta tccttatcag ctttctggcg gacaaaaaca gcgggtagcg attgccagag
    26521 cattggctat gaatccacgg gtattggctt atgatgaacc aacaagtgct ttagatcctg
    26581 aattacgcca gcaagtcgag cgtttgatcc tatcattgaa agaagatggg gtaacccaaa
    26641 ttattgtcac ccatgattta gtatttgcag aaaatgtcgg tgatcaatta ctaaaagttt
    26701 ccccaattca ttaagaaagg agaagataga gatgaaaaaa accagttttc tattcgcttt
    26761 gatcgcaggt cttcttcttt ttgcaggctg ttcgaacaaa aaaacttcag cagataattg
    26821 ggcgactatc aacgaagaga aaaaaatcgt tataggttta gacgatacat ttgttcctat
    26881 gggatttaga gataaagacg ggaagctcac aggatttgat attgatttag ccaaagcagt
    26941 ctttgaacaa tatgggatta cggtagactt tcagcccatt gactggtcaa tgaaggaatt
    27001 tgaactgaac aatggaacga tcgatttgat atggaacggc tattccaaaa cgccggcaag
    27061 agaaaagaaa gtgcaattta caaaacctta tatggaaaac gatcaagtgc tgatcacacc
    27121 taaagaatca ggaatcacaa actttggaca aatgaataat aagagattag gtgcgcaaaa
    27181 tgggtcatct ggttatgatg tatttacgaa acaaccagaa gtattaaaag agatagtcaa
    27241 aaaccaagat gctgttttgt atgatagttt taatgaagca ttgatcgatc tgaaaagcag
    27301 acgggtcgat ggtctgctga tggataaagt atacgcagac tattatttga aacaaagaaa
    27361 cgaactagaa gacttcaaca taacaaaagg ttcttatgag agtgaggatt ttgcagttgg
    27421 tgcacgcaag tcagatagta cattagtgaa aaaaatcaat gaagccctag atcaactaga
    27481 gaaaacaggt gaattccaaa aaatatccga taaatggttt ggcgaagatg ttacgccgag
    27541 aaattaacaa ataggggact gtgacaaaag acgtgtcaca gtcccttttt ccgaataaac
    27601 agtgttctcc tgccaactcc ttgtgactcg gagctgaatg gcaggctcac aacctcttgg
    27661 taaacggtgt tcaaaagcca gtttttcggt attaagccaa atttatgcaa aaatgtagaa
    27721 agccattttc acaaaatttt tcttactact cgaaactaag cgactttttc acaacctcct
    27781 ttacataatt ttattttgtt gtggaagggg tttcgtgtta ttctaaaaga ggaacgattg
    27841 aaggttatag gaaaggaaga gatgaatgga acacaaagaa cttacctcat ttccaaaaga
    27901 ttttctgtgg ggctctgctt ctgcagcgta tcaagtagaa ggagcttggc aagaagatgg
    27961 gaaaggagaa tcagtctggg atcgatttgt acggatacct ggaaagacat ttaaaggaac
    28021 gaacggtgat ttggcagtag accactatca tcgttataaa gaagatattg ctttaatgaa
    28081 agaacaaggt ctgaaagctt accgcttctc tgtagcttgg acacggattt ttcctaatgg
    28141 acgaggagaa gtcaatcaag ctgggctcgc tttttatgag agattgatcg acgaattgat
    28201 tgaaaacgat attgaaccta tgttgacttt atatcattgg gatcttccac aagcattgca
    28261 agatgaatat aatggttggg aatcacgtca gatcattgaa gatttcactc attatgcaga
    28321 aacattattt gaggcattcc gcgggaaagt ccattactgg attagtttga atgaacaaaa
    28381 tatcttcact tcattaggtt atttgttagc agcacatccg ccaggtgtga cagacccgaa
    28441 acgaatgtat gaagttaacc atattgctaa tttagcaaat gcatctgtca tcaataaatt
    28501 tcacgagtta gaaatgcctg gaaaaatcgg accaagcttt gcgtatacgc caaattatcc
    28561 aattgacagt aatcctgaaa atgttctggc tgctgaaaat gcagaagacc tgatggccaa
    28621 ttattggatg gatgtctata tgtggggaaa atatccgatt gctgccatga ggttcttaga
    28681 agaaaaagga tgggcaccaa cgattgaagc tggcgatgca gaattgctag aatctgctaa
    28741 accagatttt cttgggatca attactatca aacggcgacg aatgcattca atccattaaa
    28801 cggagttggt gctggaaaaa tgaatacgac aggaaaaaaa agttcatcag aagaaactgg
    28861 agttcctgga atgtataaaa aagttgagaa tccatttgtc gaacgaacaa attgggactg
    28921 ggagattgac ccagaaggat taaggatcgg actgcgtcgt atcacaagcc gttaccgcat
    28981 gccggtactg attacggaaa atggcttagg agagtatgac aagttgacag aagataaaca
    29041 aatccatgac gactatcgaa tcaattactt acaaagccac gtcaaagcca tcaaagaagc
    29101 aatttcggat ggagcagaag tcctaggcta ttgcacctgg tcttatacag atttgcttag
    29161 ctggctgaac ggttatcaaa agcgatatgg atttgtatat gttgatcaag acgaaacaca
    29221 agaaggatct ttggaacgat ataagaaaga cagttattac tggtaccaaa aactgattga
    29281 agaaaatgga gagaaccttt agttcacata cttaaaacaa gaaggtacgg ctggatatgg
    29341 ccgtgccttt tcttgataaa atcatttaga aattaagaaa aactgtaaaa taagctgaaa
    29401 aatgaaagaa agcgtcagtt ctttcaaaca aaactttagt tcccttactt ttttttgtat
    29461 aatgaaactt gatgaaatga gggataataa atggcacaaa agaaacggcc tactaaaaaa
    29521 aggaaaaaga cgaaaaaaac gaaaaagcaa aaacagcagc aggaacaaat gctgacgatt
    29581 ggtgttgggt ttgccttgat tttatttgct gtcttcggct ttttaaaatt aggtttttta
    29641 ggaatattga ttgcaaacgg ctttcggatc attgcaggaa atacttacca aatcctctgt
    29701 cttttattag ccgtattagg attttggatt gtcattaaga atacagagtt ttcaattggt
    29761 aaaaaccgac gctggtttgg cggcatactc ttttatttcg gtatcttatt attgttacat
    29821 gcccatttat ttggcaaact ccatactggg gaaccaaata tcatggggac gacatgggat
    29881 ttgttagcca gtgatataaa acaaagtcaa gtagacaata acgttggtgg tgggatgatt
    29941 ggagcaatcc tttatcattt tacttacttc ttgattgcac agcctggaag ttatttagca
    30001 gcagtgctat tactggcagg aggagcattc ctgttcagta atctggaagg ctatcagctg
    30061 ttaaatggta tccaatcaat tggagaacga gtacaggaat tattggaagg tgatcctgcc
    30121 aagcaagctc gaaagcaagc ggcaaaggaa gagagaatga aacagcgcgc agaagcgaaa
    30181 gaagcaaggc gcttagctgc aaaagaagct gcagaatatg agaagaaaaa agcggtgagc
    30241 cagcaaaggg aaaatactgc tgatgaatgg caggaaccag aaaatacaga gccggagcag
    30301 ctaagttttg ttccaatcga cagttttcaa gaaaatatcc atcctgcgaa tctagagaaa
    30361 ccagtaccag atacaccaaa acagaccaat acagcagaag gtttttcaga tgagttgcca
    30421 gaggatgacg gtacctcatt agaatttgag attgaagcag aacaggaaaa tcaagactat
    30481 gaattaccga cagttgattt gcttgattct ataccaactg tggatcaaag tgatgagtat
    30541 aaaaaaattg aaaaaaatat cggcgtatta gaacaaactt tccaaagttt cggtgtcgat
    30601 gccaaggtcg tcaaagccag cttaggacca tccgtcacaa aattcgaagt ccagccggct
    30661 gtaggggtaa aagtaagtaa aattgtgaac ttaacagatg atatcgcgct tgctttagcg
    30721 gcgaaagatg tccggatgga agcaccgatt ccaggtaaat cactgattgg tattgaagta
    30781 ccaaatggaa agatcagtat ggtttccttt agagaaatca tcgaagcaca accaaaccac
    30841 ccagataaat tactagaagt acctttagga cgtgatgttt ctggacgggt acaaacggct
    30901 gatctttcga agatgcctca cttactcgtg gctggttcga ctggtagtgg gaagtcagta
    30961 gcaattaatg ggattatcac aagtatatta atgcgtgcta agccccatga agtaaaactg
    31021 atgatgatcg atccgaaaat ggttgaattg aatatgtaca acggcatacc tcatctgttg
    31081 acgccggtcg tgacgaaccc tagaaaagca gcgcaagcct tacaaaaagt agtacaagaa
    31141 atggaagaac gatatgaaaa attcgcggca accggagtaa gaaatatttc tggttacaat
    31201 gaatttgttc aacagaaaaa cttggaaaac ggaacgaaac atccaacctt accattcatc
    31261 gtagtgatcg tagatgaatt agctgatttg atgatggtag ccagcaatga agttgaagat
    31321 gcgattattc gtttagcgca aatggctcgg gcagcgggga ttcatatgat cctagctact
    31381 caacgtccaa gtgtggatgt tatcacagga atcatcaaag caaatgtacc ttccagaatg
    31441 gcatttgcgg tatcaagcgg tacagattct cggacgatca ttgattctaa tggggcagaa
    31501 aagctcttag ggcgaggaga tatgctattc ttgccaatgg gtgagaataa accgatccgt
    31561 gtgcaaggag cattcatttc tgaccacgaa gtagaacggg tcgttcaatt tgttacagat
    31621 cagcaagaag ctcattatga agagaaaatg atgccgacag atgaagttga aacgacaggt
    31681 gctccagaac agccacaaga tgagctgttt gaagaagcaa aagcacttgt cgttgaaatg
    31741 caaacggcaa gtatttccct tctccaaaga agatttcgga tcggttacaa ccgagcagca
    31801 cgtttagtag atgaactgga agctcatgga gttgttggcc cgtcagaagg aagcaagcct
    31861 cggaaagtat tcattgagca ggaagaaaca tcagaaacgt tgccggacga taatgacatg
    31921 taaaacttgt gtgatgaagg gttgaaaaac gaataaccaa taaaaagaca aggcatgatg
    31981 ataccaaaag aaaaaatggt ttttttccat cttttctaat acggtatgat gtcttgtctt
    32041 tttagttttt gtgagattaa agttctattc gatttatttg tgaagtattg cattaaggaa
    32101 atgaatcatg tatagtatgt agcgaaactt tgagaaaaag agggaaaata ctttggaaaa
    32161 acagtccatt tatggcttga cgaatgaaga attaatcaat tggtttatag aaaacggcga
    32221 gaaaaaattc cgagctgccc aagtttggga atggttgtat caaaaacgtg taagtaattt
    32281 cacagaaatg acgaatctat cgaaacaatt gatcgaaaaa ttatcggagc attttatcat
    32341 caatcctcta aaacagatgg tcgtccaaga agcttcggat ggtacagtaa aatatttgtt
    32401 tgaactccct gataaaaaca tgattgagac tgtactgatg agacaagaat atggcttgtc
    32461 agtttgtgtg acgacacaag taggatgtaa tatcggctgt acgttttgtg caagcgggct
    32521 gctcaaaaag aaccgtgatc tgactgctgg agaaattgtt gcacagatca tgatggtcca
    32581 acattatttt gatgagcgca atcttggcga acgcgtttca catgtcgttg taatggggat
    32641 cggtgagcca tttgataatt acgacaatgt aatggacttt ttgcatatca tcaatgatgc
    32701 caaaggattg gcaattggtg ctcggcacat tacagtttcc actagtggat tagcacacaa
    32761 aatcaaggaa ttcgctaata acggcttgca agtcaatctg gctatttcct tgcacgcgcc
    32821 gaataatgag gtgcgaacat cgatcatgcg gatcaaccga agtttcccaa ttgagaaatt
    32881 gatggaagct gtggatgagt atttagagaa aacaaaccgt cgaatcacgt ttgaatatat
    32941 catgttgaat caagtaaatg atagaccaga gcatgcgcaa caattagcag atctattaaa
    33001 agataagaaa aaattagcat atgtcaactt gattccatat aatccagtca gtgaacatga
    33061 tcaatattct cgcagtccaa aagctgatgt attgagattt tatgatgtat tgaagaaaaa
    33121 cggcgtaaac tgtgtgatac gtaaagaaca tggtacagat attgatgcag cttgcggaca
    33181 attgcgaagc aagcaaatga aaaaaacagt tgccaaataa tcgtatccta ctcgtttttt
    33241 caattaccat aaaaggagca gttttcttaa ttaaggaact gttcaactta aataagagaa
    33301 aagattctat agcgaaagct ataaagcttt ttttgacaag aaaaaccctc atcaagcaaa
    33361 gaaatgtcct caatttgatg acagcctgct tgatgggggt tttagcaatt atttaactga
    33421 ttttcttata tccttgctgg ctttcttgtg tagcatgata agtttctacg tcaagaatgg
    33481 cctgtttaaa tttttctcgg gtgatttcat aaggcacatg tttaatatca tttgttgcca
    33541 tggctttatc taagattgct tctaaatcgg tcgtgacatt gagattcatg tcggttaacg
    33601 atactggaag ttggttcttt ttcataaaca catagatttt atcaaacaaa tcgaactgct
    33661 gatccatcat caataaggtc agtacaccat aactgacaga tgctccgtga aggtatccct
    33721 ccattttttc tagaacagtc gtaccgtaat agaatgagtg tgccatagca gaattatagt
    33781 tattgttaac taaaacagaa accaatccgg tagtaacaat gatatccgaa acgacttgtt
    33841 caattgcata tgagggacga tcgtcggcac tatctttcaa tgcttgcgta ccataagtca
    33901 agagacgatc aatgcacatt tttccgattt ggattcctaa tctatccgtg tagcctagat
    33961 catcgccacg agcagaaaaa gtagattcga cttctttgga taaagcatcg ccaatacctg
    34021 cccataagta tttatcaggt gcttcggcaa taattgctgt atcaatgaag gtgtgtacag
    34081 gtggttttga aggaaagaag agtccgtcca ttacgccttc ttcgttatag atgacacata
    34141 ccgcagtgac gggcgaacag ttcgaaccaa tagtgggaaa ggtgaagact ggtttagcta
    34201 gtttttcacc aatgactttt acagtatcgc aagcacggcc accaccgact gcaaacagga
    34261 cgtctgcttt ttggacactt tcatgctgga tcaactgttc aatatttcca taagttgaat
    34321 tacccccgta ccagagactg gcgacaacct ctatttcctg tgtcaaagct acttttattt
    34381 tgttttctgc ctttgaaagt gcagtcttcc ctccaatcag cactactttt gtcccatatt
    34441 ctttgacgat tccatttgct ttttcatacg catctgttcc aatggtataa tttggtaaga
    34501 aaacactttg actcatattc atttccttct ttcttcgttg aaacgggtta aaattttaaa
    34561 gagtgatcga tctgactgcg gctgtagatg gagtagaggt gaacctaaga caggataagt
    34621 gatgaataag gtggcatgaa caccttgaaa tcatcaataa gtatcctaaa aacgaacaag
    34681 tacaaaaaca ttgaaaacga caataatgaa tttttgttcc tcttagtatt tctaccgatt
    34741 ccctttgctt ttttgtaatt atctaaaaat tctaaaaatt aagattatta tatgaaaaca
    34801 ggttttattt gtcaattcat aattggtttt ttttaaagca aaagataatc tatatcatga
    34861 tgaacaaaca gagttttttc ctccaattga tggctttcct gatctttcct attttgaata
    34921 aagtgtttct gtataatttt ttattttata agtaagataa ataatgaaag aatagagata
    34981 ctgattggta gctaaagatt tttgagcgag aacatttcgt tttttcaaaa ggactgttag
    35041 aataagcaag aacaaggggg aagacgtatg ataatcagtg aatttgaccg taataatcca
    35101 gtattgaaag atcagctttc tgatttactg agactgactt ggccggaaga atatggagac
    35161 agctcggcag aagaagtaga agaaatgatg aatccagaac gaatcgcggt agcagcggta
    35221 gaccaagatg agttagtagg atttattggt gcaatccctc aatacggtat cacaggttgg
    35281 gaattgcatc cattagttgt agaaagctcc cgacgaaaga accaaatagg tactcgatta
    35341 gtcaattact tagaaaaaga agtagcttcc agaggaggaa tcacgattta tttaggtacg
    35401 gatgatttag accatggaac aacgttaagt caaacggacc tgtatgagca tacatttgat
    35461 aaagtggctt ccatccagaa ccttcgtgaa catccgtatg aattctatga aaaattaggt
    35521 tataaaatcg taggtgtctt accaaatgca aatggctggg acaaaccgga tatttggatg
    35581 gcaaaaacga ttattcctcg accaaattct caataataaa aagaggttgt gacagaagta
    35641 atcagcttca agaaataaga aggaatttac gaaaattact tcttgaattt ttgtgaaatt
    35701 ccaacttatt tccgaagaag ctacttttgg aacaccgtgt accagaggtt gtgaaaaagt
    35761 cgcttagttt cgagtagtaa gaaaaatttt gtgaaaatgg ctttctacat ttttgcataa
    35821 atttg
//
LOCUS       DACCYH010000021        35333 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.021, whole genome shotgun sequence.
ACCESSION   DACCYH010000021 DACCYH010000000
VERSION     DACCYH010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.021"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            30..329
                     /locus_tag="IVY85_000981"
     CDS             30..329
                     /locus_tag="IVY85_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8044013.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            594..1469
                     /locus_tag="IVY85_000982"
     CDS             594..1469
                     /locus_tag="IVY85_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044014.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            1466..1684
                     /locus_tag="IVY85_000983"
     CDS             1466..1684
                     /locus_tag="IVY85_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044015.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            1671..4298
                     /locus_tag="IVY85_000984"
     CDS             1671..4298
                     /locus_tag="IVY85_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044016.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            4313..4531
                     /locus_tag="IVY85_000985"
     CDS             4313..4531
                     /locus_tag="IVY85_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044017.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
     gene            5713..6031
                     /locus_tag="IVY85_000986"
                     /pseudo
     CDS             5713..6031
                     /locus_tag="IVY85_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348881.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6050..6364
                     /locus_tag="IVY85_000987"
     CDS             6050..6364
                     /locus_tag="IVY85_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044018.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(6417..7190)
                     /locus_tag="IVY85_000988"
     CDS             complement(6417..7190)
                     /locus_tag="IVY85_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044019.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            7295..7540
                     /locus_tag="IVY85_000989"
     CDS             7295..7540
                     /locus_tag="IVY85_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8044020.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            7696..8547
                     /locus_tag="IVY85_000990"
     CDS             7696..8547
                     /locus_tag="IVY85_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044021.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            8580..9254
                     /locus_tag="IVY85_000991"
                     /pseudo
     CDS             8580..9254
                     /locus_tag="IVY85_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9511..10710
                     /locus_tag="IVY85_000992"
     CDS             9511..10710
                     /locus_tag="IVY85_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8044022.1"
                     /translation="MLFTSGPDRAFERLMQEKPGFDHYDGGGADAPEDCGTCRFYRPD
                     WKYQFCQFAECPYQPGKLTDYAAVKFTVKGANNAMAVFRVEKNKGYTVMSNHHLRNKN
                     LTLKAKGLLSQMLSLPEDWDYTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDA
                     KGKMADMVYTIYEQPVLENPVLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLS
                     KKEKIITDGQSTHSIPIPSPTPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEY
                     AYLIQDRSIDREQLDEIVDLMLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRF
                     VLDCLRENTTKVRNIKQYLRAMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYS
                     CGEGESL"
     gene            10732..10944
                     /locus_tag="IVY85_000993"
     CDS             10732..10944
                     /locus_tag="IVY85_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP8044023.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            11019..11495
                     /locus_tag="IVY85_000994"
     CDS             11019..11495
                     /locus_tag="IVY85_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAP8044024.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            11492..13300
                     /locus_tag="IVY85_000995"
     CDS             11492..13300
                     /locus_tag="IVY85_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP8044025.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            13377..13592
                     /locus_tag="IVY85_000996"
     CDS             13377..13592
                     /locus_tag="IVY85_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein"
                     /protein_id="HAP8044026.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            13684..14547
                     /locus_tag="IVY85_000997"
     CDS             13684..14547
                     /locus_tag="IVY85_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044027.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            14578..15120
                     /locus_tag="IVY85_000998"
     CDS             14578..15120
                     /locus_tag="IVY85_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HAP8044028.1"
                     /translation="MFISTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVE
                     RLASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTR
                     GNAEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFAR
                     QKTPGWDVWGNEVECDLILT"
     gene            15134..15556
                     /locus_tag="IVY85_000999"
     CDS             15134..15556
                     /locus_tag="IVY85_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP8044029.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            15486..17885
                     /locus_tag="IVY85_001000"
     CDS             15486..17885
                     /locus_tag="IVY85_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP8044030.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            17917..19908
                     /locus_tag="IVY85_001001"
     CDS             17917..19908
                     /locus_tag="IVY85_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP8044031.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            19931..20182
                     /locus_tag="IVY85_001002"
     CDS             19931..20182
                     /locus_tag="IVY85_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAP8044032.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            20172..21401
                     /locus_tag="IVY85_001003"
     CDS             20172..21401
                     /locus_tag="IVY85_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAP8044033.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            21398..23479
                     /gene="topB"
                     /locus_tag="IVY85_001004"
     CDS             21398..23479
                     /gene="topB"
                     /locus_tag="IVY85_001004"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8044034.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            23628..27548
                     /locus_tag="IVY85_001005"
     CDS             23628..27548
                     /locus_tag="IVY85_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAP8044035.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            27549..28493
                     /locus_tag="IVY85_001006"
     CDS             27549..28493
                     /locus_tag="IVY85_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044036.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            28497..28700
                     /locus_tag="IVY85_001007"
     CDS             28497..28700
                     /locus_tag="IVY85_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044037.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(29000..29236)
                     /locus_tag="IVY85_001008"
     CDS             complement(29000..29236)
                     /locus_tag="IVY85_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8044038.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(29419..29790)
                     /locus_tag="IVY85_001009"
     CDS             complement(29419..29790)
                     /locus_tag="IVY85_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044039.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(29787..31115)
                     /locus_tag="IVY85_001010"
     CDS             complement(29787..31115)
                     /locus_tag="IVY85_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8044040.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(31076..31405)
                     /locus_tag="IVY85_001011"
     CDS             complement(31076..31405)
                     /locus_tag="IVY85_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP8044041.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(31663..32033)
                     /locus_tag="IVY85_001012"
                     /pseudo
     CDS             complement(31663..32033)
                     /locus_tag="IVY85_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            32178..32363
                     /locus_tag="IVY85_001013"
     CDS             32178..32363
                     /locus_tag="IVY85_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8044042.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            33306..33965
                     /locus_tag="IVY85_001014"
     CDS             33306..33965
                     /locus_tag="IVY85_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAP8044043.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            33968..35311
                     /gene="vanS"
                     /locus_tag="IVY85_001015"
     CDS             33968..35311
                     /gene="vanS"
                     /locus_tag="IVY85_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP8044044.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
BASE COUNT        10008 a         8138 c         8862 g         8325 t
ORIGIN      
        1 gaataattaa ttaaaaagga gaggtttttg tggaagcaat aaaattaatg atttcagaag
       61 aacaggagga aatgtttcta aagcagtttt atcaaattgc tacaaaagct attgaaaagg
      121 ctcaagaaaa tgcggggatg acaagggaat atctcaatca aaaagaaatg gcggaatact
      181 taaatgtgag tgaaaacttt ttaaaaaatg ctgtgagaaa tgagggattg cccgtagttg
      241 aattaggggc aagaaaattt tattcgaaaa aggcagttaa tgagtttatg tttgctcgaa
      301 tgaaaagagg ggaaaatatt ggaagatgat gaagtagaaa ttataaaaaa atgctttaaa
      361 aaaagaaaaa aagtgttccc aaaagtttgc agactggggg aacactcaat aataaagtgc
      421 tgagaaaact ttatttatag ctattatatc aaaaaagaag gtgaaaaggt gttttcttct
      481 ggaaatgttg tagttgacaa acttagagaa ataaattttg acggaaatgt aatccacaca
      541 catggtacga tcaattaaga aaaaaaacga aaaaaggtgt tgagaagccc tatttgaatg
      601 caatagttat cttagcagaa attacctatt ggtatagacc taaaaaaatt tttaacgatg
      661 atggtcagct acttggttat caaaaaaagt ttcttgaaga tattttgcaa aaatcatata
      721 agcaattaag caaaaaaaca ggattatcag aacgagctat aaaagatgca attgtgtact
      781 tagagaaaaa ggggattatc aaaagggttt ttagaaatat ttattatgag aaaagagcag
      841 ttacattatc aaatgttatg tacattgttt taaatgtaca taaattgata gaaattactt
      901 ttcctacttt ggattgcgaa aaaaaggagt atatgccgaa aattcgtcag aggggtatcg
      961 aaaaaaagag atatgtctct ttaaaaaaag gtagaggtat gtacaaattt gtatatacgt
     1021 atacaaagac tacttcacag aacatatcaa agactactct cgttatggaa gaagaggata
     1081 ttaatataaa gaggatttac agtaagatta gtgttcttta tgacgcaaat aacgaagatg
     1141 gtcgaaaatt atttcaagtg tatcactatt tgagaaacgc taatgtagat acagaagata
     1201 tcagaaaaat cattgaatat ttatctgaaa atactcagta tcttattgct gagtgtatta
     1261 taaaacaaca tgaagcatgc ttttttcaaa gaaaaactga agaagggtta tacgattatg
     1321 taacatattt tttgaatgga cttcagaaaa gatataaaag tcaattttta gaaaatgtag
     1381 agcctataga tcagttcaaa aaagctttcg aaatcaaaga gttacctgaa attactatgc
     1441 ataattggtt gaaaggagaa gaatcatgat taaaaatcgc ttaatagatc aaatgtggtg
     1501 tcttgatcaa aatacagata ctctcgaatt aatcgagagt atctgcttca acacagtagt
     1561 tctcgattta agagctgaaa atgacaattg tgtaactcat atcataatta atcaaaaaat
     1621 ggctcagcag ctctctgaga gcctaagaaa atgggctgaa ggagaaaact atgaaagcaa
     1681 ttaaagaaaa aatcgttagt agaaaaataa actggttagc actcagtgta cttgttttaa
     1741 tgattagcgt tgtttttaca agtaatattg ttgaagctag cattttcaat tggtttgatg
     1801 acacaaaaag tcaaacagag tttttgaata attctcttta tagaccttac ctaaaaaaat
     1861 acaatggaga tttccttagt cagttcggga tttggcttgg atgggctgtg gtaaaaggca
     1921 tgtttactgt gactgattcg attcaaaata tgattccaga tgtgttggat ttgttcaatt
     1981 tcattgaaag tacagggcta aataatgttt atcaatcagt cataaataca attgtcgttg
     2041 gattaatgat cctttcattg atgtttgttg gctacaagat gattactgga aaaggaacaa
     2101 tagatttaag atctgttgga atgaatatag tgatgtctgt agcgttaatt cttttaatgc
     2161 caacaatgat ttctagcggt attcaatttg ccaaaatatt ttacaatgat gctactacga
     2221 ttacaaattc agatgacgga gtcgcttggt cattgataaa acaaggagta acagatctgg
     2281 catatattaa taaaacagat caatactcaa gcattgataa aacggaggat cgaaataagt
     2341 taacacgaaa aaattttcaa cagacagatt taacacaagt gttgacggat aaagtgattg
     2401 ataaattgga aaaagaaaat ccagctgcgg ataatcttcg ctatgaatta gttgaaaatt
     2461 ctaataatga atttgttgcc acaaagtttt ctgataactt cttatcaaca ttttctgata
     2521 gcttgaaatc aggttattat cgatatcaag caaatctttg ggggatttcg attgggttga
     2581 cagcgttggc aatcgcctat gtgttcagcg cgtttgtgat cattacagca atattagaac
     2641 ttgcatttaa acgtgtgtta ggtgttcttg tttttgcgac agatattgaa acaggacaac
     2701 gttcaaaagt tgttttatcc gatattttac aatgttactt aacagttgga tttcaagggt
     2761 ttggactatc tatgtttgct atgtttatca attttttaaa tgctggacaa ggtatctcca
     2821 caaatatttt tataaaaaca attgcttata tctgtgctgt gtttgtgctg ataaaaggca
     2881 gtggaacagt catgagatac tttggtgttg atattggact caaagaaggc tatgggcaac
     2941 ttgcttctgc ttttggtatg ggcgcaatgc tgtttagaaa aggatcaact ggatttaatc
     3001 gtgctaaagg aagcggaaat ggaaatggat cagacagtgg agaaggagaa gatcgaaaac
     3061 cagaaaagaa tttcggcgaa actctatcaa aaaaagctgg caaaactgga agagctttgg
     3121 gatatgccca tgaacgtgga ttatcaggtt tagcttcaga tggagctaca atggctagtg
     3181 aaagagcaat aaaaccattt aagagtatgc gtgatatggc taatgatacc aagaataagt
     3241 tcaaagaagg tttagatgat ggtacagtgt ccgctattaa taaaaatagc aaacctatgt
     3301 tagctaaaaa taaagaagat gaaactggaa aatatgctga ttccatgcca acacgtcttt
     3361 cggatagaaa agatggtgct aaggttgaaa atgccgatag aattatgtct agttctgaac
     3421 gtatgcgtga agcaatgaaa aataatgctg aatcgaataa taatcctgta agtgcaattc
     3481 agcaaaaagt tcaacaagat attgaagaac gtaaaaatgc tatgcacggt caagctaagt
     3541 ctgctgaaga attaattaat cagaagcgac aagaagctaa atacacgcct gaagcaatga
     3601 atcgtgagga aatgttaagg aaacgtgtag aagggcgtac aggagctaat acggacgaaa
     3661 aagaagcgtt gaccaaagaa agaatccaag aagctaaaaa ctcaaatacc ggattagaaa
     3721 aattggtcaa agaaaatcta caaaattcta caagtagtca aggtggccga tcagtagatg
     3781 tccgagaaaa tattcaaggt tcattgaatg gtcatgtcgg acggaatgtt gatgtaagag
     3841 agaatttaca aagctcttca aatggtcaag gtactaagac aattgatgtt cgagaaaatg
     3901 tacaaaattc tacaagtagt caagggttta aatcaatgga tgtaagagag aatgtacaaa
     3961 attctacaag tagtcaaggg tttaaatcaa tggatgtaag agagaatgta caaagttcta
     4021 caagtagtca aggattcaaa acagtagatg ttcgggagaa cgttcaaaaa gatagtggag
     4081 aaatgaaaga aaaaacacaa aaaatcaata tcgttgaaga cagaaagtca gcgaaaaaat
     4141 ttgattcaaa tcatgaaaca gtgattattg atagtgaaat ccgtgaaaat gataagggat
     4201 caaaaccaag acgaagattt acctatgaag acaacgagtt attcagagat acacttaatg
     4261 atcctaatcc gcttttcgat aatctattaa aaaaataaaa gggggaattg tcatgaaatt
     4321 aattctaaag attagtacgc atgatttctt agaacttgac gaattatgtg aatcagtaaa
     4381 gaacgcaaca aatgaaaaaa tcacggtcac taattatatt gaactgatga ttcaagaaga
     4441 attaaagatg aaacatgaga taaaaaacaa cttgaaatat tgttatctag aaaaaagtga
     4501 agagataatc catgaattaa atggctttta aaaattgtat ttgacatgaa tttacgacaa
     4561 ggaattagtg gggattaagc gattattctc ttccacttta gagaaataat gggttaaaaa
     4621 gccagaatac gtagagcaat attgacaacc tataaaggag ctattgttga aaattgaagt
     4681 aacaaaaaga taaagagagg tttacatcta tcagattaaa agtgatagta cacccatgaa
     4741 acctgcaaaa gagttgaacc taatgtatat cggtttaact ctttttattt gcaaattaaa
     4801 aaaatatcac cgaagtgacg aatgaaacta tgaccaaatt aagcaagaaa atatatcttg
     4861 caaataaagt agtataaaaa gatattgata aagccaatca aaactttgaa tttcaaacgt
     4921 tagataatca aacagataca aaaaatgaaa tcagttctac gcgtacgatt attaaaaata
     4981 ataaattttc actagaagaa aatggaaata aaatcaaata attaggaaga aaaaacaaag
     5041 caattagaaa agcctgcaag caaatttgag taatcgagat ccaatttttt taaggaagtt
     5101 taacaaaaat atgtaattct attatcacta ttaaagagaa agttggagcg gaatataaag
     5161 aaaagagaaa agtaattaaa cgcaaaaaaa tgtagaactt aaaaaatata aagaggaaca
     5221 accgttaaaa ttaggaatga tgttgtgtaa acattataat tgctatgata taaatgaagt
     5281 gttaaagaaa gcaaatttaa accaattttg tgaacagaaa agccttagtc actatgacca
     5341 aaactacgga cgaaactatg accaaaagta ggtactaaat tatgaccgaa aataggtcca
     5401 aaatggattt ttttatgata tcaaaaatta tgaaatgatt ttgaaagaaa accagttggc
     5461 acagccaact tagccacctt cctgtaggaa ggtgctatca gttttccctt atgctctttt
     5521 ctttcttttt tgtttcatat gtcaaatgcc attttgaaat gtaccatctt aagaaattta
     5581 tccttaaaaa caattgcgtt attggataaa aagatgctac attatcttaa aaaaggaatc
     5641 aaatatgatg aatttagtgg ctagcatatc gtgtgattaa gatactttat agtgtaagtt
     5701 gaaggagaaa aaatatttaa aatatacttc ccatttcagt gggaagtaaa attaatcttg
     5761 ttgtttctac tagtaatgta tctttttgga aactatgtaa cgatgtaaac aagatttttt
     5821 tcagaagaaa aaacgataaa agttgtgata aacgatttaa aaaataggag aaaatatatc
     5881 aagcgcatgg tatgaaacgc acatttaaaa agattcgact aactaaaaaa ataagtttac
     5941 aaagcattgt tgtgtattac aagtgatcta tacactgaaa aatgatagag aaaatctgat
     6001 acgtccgcaa caatggacaa ttgaaaatga aagtgaggaa attggaagaa tggctgatga
     6061 aaataaagtt gttccgcttg aaacactagt gataaataag gaagagtttg agcaactttt
     6121 tcttgatttt tttgttcaat atgacaggat ttatgatttt agttcgtcac tagatttatt
     6181 tgaattagag gcagaaataa tagaaaacag tcgagaggag aatggtaaaa tcatcatttc
     6241 agtagaaaaa ttcaatgaat tattctcaga aacatttgaa atagatacag attattatca
     6301 accacaagaa gttttccaat catttagaaa tcagattatt cgctcaggta agaccgtaaa
     6361 ctgaaaaaca ataaaagaaa cgttgaaaga ataattattc cttcaacgtt tcttttttag
     6421 aatgttttaa tgaccaaagc agtttcattg tcaggcttag gtaattcatg aaatcccgta
     6481 tttatagtgc tcaattgatc aactaaccat gttttacttt ttgaattgta gataaatcta
     6541 aaattaatat cgttattatc gtgttgaagg ggcgtctcca aataatctgc tgtatattca
     6601 tagtcaacgt aacctgctaa tacaaaagag agtatccctt tatttaatgt tagatttaat
     6661 ggataatcat acgactttat ttttttcaac ttcatattgt acatatcttc agccataaaa
     6721 gttgagttcg tgatttcatc ttgggtctca ttaaaaaagt tatccgaata atcgcttatt
     6781 tttgaaaagt ctttattggc accagcctca aaaaattgtt caactttttc agtataaagt
     6841 tgtgctaacg ctcctttttc aatattaact ttaatatttt catgtctatc aatttgtgtg
     6901 ctgttcagta agggaagatc agcttttagt tttatatcgt cttgatttct ttcaatttca
     6961 attggcaaaa tcttttcagt agattcagtt tctgataaag gaacatctag ttttttatca
     7021 tgataataag cttcaaatcc atctggaacc ggaaaaaaat gtattgggta attcttttct
     7081 gaggttgttg tactagtttc cgtacttgta gaacttgtgt cagaagtggt acacccgctt
     7141 atagttccga tgagtaaagc gcaagttaga atgattgcaa atttcttcaa aaaaattcct
     7201 ccttaaaatt gaatatggac taatactagt cccattgtag cataccgaat ctttaagatt
     7261 aataagaaaa aggactaatt ttagtccaaa aggtgtgcta ttcttgaaag aggaaaaaat
     7321 tattgataaa gaaaatgtaa tcggtagcaa tattagacga gtccgattag aaaaaggaat
     7381 tggtcaaacc gagctgatta gagacctaca gcttcgtaaa attgctataa ctagggaaac
     7441 gttggttaag attgaaggtg gccgccaaca cattaagttg gatcaattga aagccatcaa
     7501 agatattcta caggtgtctt atgaagattt gcttcaatga ctacttgtag tcatttcgtt
     7561 tgaaattatt atgttacacc cttatgtgtc acgaagaaat actggtataa taatgaatgg
     7621 gatatgtaac gatagggtat actgttatgt tgcctaataa attgtggatc aaaatacaag
     7681 aatggaggga tatccttgta taaggcatat gaattggatt ttacaaatgc tgacttaaat
     7741 gcattctcct caagctcgca atcatataca catgtagtaa atcaaataga tcacaaccaa
     7801 aaatctatta acaagagaat agaaaatcta tttgaaagtg aaaatgattt agttgattat
     7861 tattctaagg acagcaaaat tctagatgca gacgaaatta ttaaagattg gtttccaact
     7921 ataaaagcag atatttttat atctcactct catgctgatg aaaaattagc tatacgtttt
     7981 gcatcatggc tatttgaaaa ttttggtctt actgcattta ttgattcttc ggtttgggga
     8041 tacagttctg atttattaaa aaaaatagac caaaaatatt gctataagga acaaacaaaa
     8101 acatatgatt atgataaaag aaatgtaaca actagtcatg ttcatatgat gctttctaca
     8161 gctttaaata atatgattga tagtacagaa tgtctgtttt ttcttaatac acctaattca
     8221 attagtttga gcaatgaaat cactaatgaa cagaaattta cgtattcgcc atggctatat
     8281 agcgaactga cgacagcttc aatagtggaa aaaaagaatc cacgattgga aagtaatcct
     8341 caaatgagca ccgaggatgt tagaagcatt ataaagcact attcttcgca agaaaatctg
     8401 caaataaaat attctttaaa tttaagtgaa ttaacttatt tacgaaaagc tgatttgaaa
     8461 aaatgggctt tagatgctgt ggaatgcact aaaggaagta atacattaga tattttatat
     8521 aaaaacacca ttttcactaa tctctaatca atttactgaa tcgaaaggaa ccgggtagaa
     8581 tggataagaa agttttaata aagaggttct ctgaagctat tgaaaaagga aatgcagcaa
     8641 tatttgcagg tgcaggatta tcgatgtcac aaggatatgt aagttggccg gaactactta
     8701 atgatcctgc aacagaaatc gggttggatt ctaaaaaaga aacagactta gttactctgg
     8761 ctcaatatta taaaaatgaa aatggtggat caagaggaat tttgaatcag atacttatgg
     8821 ataattttgg tgaagaatta gaaatatctg aaaatcatcg aatactagca agtttaccta
     8881 ttgaaactta ttggacaact aattatgatc atctaataga aaaatcaatc cgagaagctt
     8941 ataaaaaccc tcaggttaag aaaaattata ctcagttagc aacaactaat ccaaatgtgg
     9001 atacaattgt ttataaaatg catggtgata tagatgatgt cagttcgaca gtaataacaa
     9061 gagatgatta tgaaaaatat gatgattcgt acgcgttatt taaagaaact ttaaaaggag
     9121 atttgttaac aaaaactttt ctctttttag gatttagttt tacagaccct aatttagaaa
     9181 gaattttatc agatataaga tgggttttga gagaaaacca aagacctcat tattgtataa
     9241 tgagaaaaat attatagaaa aaattttagg ttatcgctga aagccccgga aatacgggca
     9301 tttcgcagga tatgagaact caatttacta ccaatttact actttcggat tgagccgcat
     9361 atatgctgaa ccatactgct aatacccgcc accccaaata cggcacatca taaattgaat
     9421 acgacaccgt aaacgcggcg ttcctctttc ccgtcatggg agaacaggac gccgcttttt
     9481 tatgcccaaa tagagaaagg aggcgacgcc atgctattta cttccggccc tgaccgggcg
     9541 tttgaacggc tgatgcagga aaagcccggt ttcgatcact acgacggcgg cggggcggac
     9601 gcgccggagg attgcggcac ctgccgattt taccgcccgg attggaaata tcaattctgc
     9661 cagtttgcag agtgtcccta tcagccgggc aagctgaccg actatgcagc ggtcaaattc
     9721 acagtgaaag gagcgaacaa cgctatggca gttttccgtg tggaaaagaa caaaggctat
     9781 acggtgatgt caaatcacca cctgcggaat aagaacctga ccttaaaggc caagggcctg
     9841 ctgtcgcaga tgttgtcctt gccggaggat tgggactata ccctaaaagg actgtcctat
     9901 atcaaccggg aaagcatcga cgcgatcagg acagcggtat gggagcttga aaaagctgga
     9961 tatatccggc gtgagcaggg ccgtgacgca aaaggcaaaa tggccgatat ggtctatacc
    10021 atctacgaac agccggtatt ggaaaacccg gtattggaaa atccaacatc ggataacccg
    10081 gtgttggaaa atccgacatc ggataatccg gcgtcggaaa atccaacgca attaaataaa
    10141 gaagtacaaa gaactgactt atcaaaaaaa gaaaaaataa ttacagatgg acaaagtacc
    10201 cattccattc ctatcccatc cccgacccct gcccctatgg agggcgaggc agcggctacg
    10261 ccgccggaac ggaaaaggac aggaaaggat gaggctgtac ggatttatcg ggaaatcatt
    10321 ttggagaaca tcgagtatgc ctatctcatt caggacagga gcattgaccg tgaacagctt
    10381 gacgaaatcg tagacctgat gctggaaacc gtctgcaccg cccgaaagac aatccgtgtc
    10441 gccggggacg actaccctgc cgagctggtg aaatccaagt acatgaagct gaccggcgaa
    10501 catatccgct ttgtccttga ctgtctgcgg gagaacacaa cgaaagtccg caatatcaaa
    10561 caatacctgc gggctatgct gttcaacgcc cccagcacca tttccaacta ctacacggcc
    10621 cttgtcgcgc acgacatggc acagcccgat tggggaaagc ctaaatccgg cctgccggac
    10681 tattcctgcg gcgagggcga aagcctgtaa cgagaaaagg aggacgaccc tatgaagcag
    10741 ggcgcattga tttttgacga gcgaacagac cgttacgaca tccgctttga tctggccgac
    10801 tattacggcg gcctgcattg cggggagtgt ttcgacgtga tgatcggcgg caaatggagg
    10861 cccacccgca ttgaaatggc cgaaaactgg tatcttgtcg gcatccgggc cgacgacctt
    10921 tccggcctgc gggtgcggat ttgacctata tctacctgcg gcgtctggtg gttccgcctt
    10981 agacgccgct tttgactttc tgacaaggag ggatgcgatt gcaggaagaa gttactcaaa
    11041 agaccattgc cctgtccatg aaaacgggca agctcacggc tcaagtgctg caagcggcgc
    11101 tgaaaaagta cctgcaacac cgggccaagg gcaaaaacac gctgcaccac ggccagcaga
    11161 gcctaaagca gcttaaaaag catggggctg atctctccaa cattgagatc accgaggcca
    11221 acattggagc gttcaagcca tgcgccaaga aatacggcgt ggattttacc ttacgaaaag
    11281 ataagaccac ccagccgccc cactacgttg tgattttcaa ggccaaggat gcagataacc
    11341 ttgatctggc gtttcgggag tttaccgcca agacgctttc caaggagcag cggccctcta
    11401 tccgcaaggt tttgtccgct gtgaagcaaa aggcggcggc ccagaccaag cagcgggcca
    11461 aggagaaaat caaggaaagg gggctggaac tgtgaagcct gacgtaaaga aattgctgat
    11521 cctgaacctc ccgtatctga tttttgtgta cctgtttgcg aaatgcgggg aggcgtaccg
    11581 gctggccgct ggtgctgacc tgtcggggaa gctcctgcat tttgcggatg gttttgcggc
    11641 ggcttttgcc aacccgctgc caagcctgca cctgtttgac ctgtgtatcg gtatcgccgg
    11701 tgccgtgatc gtgcggctgg tggtgtactg caagagcaag aacgtgaaga aataccgcaa
    11761 aggcatggag tatggcagcg cccgctgggg gacggccaaa gacatagcgc cttacatcga
    11821 ccccaagttt gaaaataata tcctgctcac ccaaacggaa cggcttacca tgaccggcag
    11881 gcccaaagac cccaagacag cgcggaataa aaacgtgctg gtgatcggtg gcagcggttc
    11941 agggaaaacg agatttttcg tgaaacccaa cctgatgcaa tgctttccga ccacggacta
    12001 tccgacttcg tttgtggtga cagatccgaa aggcactttg gttctggaaa ccggcaggat
    12061 gttccagcag gcgggctacc gcattaaaat cctgaatacc attaactttt ccaagtccat
    12121 gaagtacaac ccctttgtct acatccattc ggaaaaggac gtgctaaagc tggtaaatac
    12181 cctgatttcc aacaccaagg gcgaggggga aaagtcagcg gaggattttt gggtgaagtc
    12241 ggaacggttg ttttattcgg ccctgatcgg ctatatctgg tacgaggccc cagcggagga
    12301 aatgaacttc tcaacgctgc tggaaatgat aaacgccagt gaagcccgcg aggacgaccc
    12361 ggagtttcaa agccccgtcg atctcatgtt tgagcgtttg gagcagaaag accccgacca
    12421 ttttgcggtg cgccagtaca agaaattcct gctgtccgca ggcaagaccc gttcttccat
    12481 cctgatttca tgcggtgcca gacttgcgcc ctttgacatc cgggaggtac gcgagctgat
    12541 ggaggacgac gaaatggagc tggacaccat cggggacgaa aagacggtgc tgtatctcat
    12601 tatgagcgat acggacacga cctttaattt cattctggct atgctccaaa gccagctaat
    12661 caacctgctt tgtgaccggg ctgatgatgt gtacggcggg cggctgcctg tccatgtacg
    12721 cctgattttg gatgaatttg ccaacattgg ccaaataccc aatttcgata aactcattgc
    12781 caccattcga agccgtgaaa tatcggcctc tattattttg cagagccagt cgcagctaaa
    12841 agcgatttac aaggacgccg ccgagatcat ttcggataac tgcgatagtg tgcttttcct
    12901 tagtgggcgc ggcaaaaatg ccaaggagat ttccgatgcg ctggggaaag aaacgattga
    12961 cagtttcaac accagcgaga accggggaag ccaaacgtcc cacggattga actatcaaaa
    13021 attaggaaag gagttgatgt cccaagacga aattgccgtg atggacggcg gcaagtgtat
    13081 cttgcagcta cggggtgtgc gcccgttttt atcggagaaa ttcgatatta ccaagcatcc
    13141 ccggtacaaa taccttgccg acgccgacaa gaagaatacc tttgacgtgg acagattttt
    13201 gactatcacg cgccggaaac ggcagcaagt ggtaacgcag gatgaagtat tcgacctgta
    13261 tgaaatcgac ctgtcggacg aggacgccgc cgctgaataa acagcggctt ttttcattcc
    13321 aacaacaaaa tcaaaaaatc attatgagcc ggaaaccggc agaaagaggt aaatttatgg
    13381 cattttttaa ttccgcagtt actactttgc agactatcgt gatcggactg ggcggcgctc
    13441 tgtgcgtatg gggcggcatc aatctgttgg agggatacgg gcaggacaat ccgggggcaa
    13501 aatcgcaggg tgtcaagcag cttgtcgctg gcggcggcgt ggccctgatc ggtgtcactc
    13561 tggttcccct gctgtccggg ctgctgggct gatcctggcc tttgtccctg tccaaaccct
    13621 taactaatcg cctccgcgtc ctcccggcca gaccgggagg gcgcgggaaa ggaggtgcta
    13681 cccgtgatta gtgaaatcat tgaagaatgg ataaaaggca tcctcatgga cggtatcacg
    13741 ggcaacctgt ccgggctgtt cgatacggta aatgccaaag tcggagaaat cgccgccgac
    13801 gtgggacgaa ccccgcaggc atggaacagc ggtattttca atatgctgca cagcctgtcg
    13861 gaaaccgtga tcgtccccat agccggggcc atactcgccc ttgtcatgtg ctatgagctt
    13921 atccagatga taacggaaaa gaacaatatg cacgactttg acagctccat gttcttccgc
    13981 tggatattca aatccgcgtt tgcaatcctg atcgtaacga acacatggaa catcgtcatg
    14041 ggcgtattcg acgccaccca aagcgtcgtc aatcaaagcg ccggtgtgat tatcgggcag
    14101 acatccattg attttaacac cctgatacca gaccttgaaa gccaattgga ggctatgagc
    14161 gtgggcgctc ttttggggct gtggttccaa acccttgttg tgggcctcac catgaatatc
    14221 ctttccatct gcattttcct tgtgacctat ggccgcatgg tggaaatcta tgttgtcacg
    14281 tcgctggggc ctatccccct tgccacaatg ggcaacgcgg aatggcgcgg catgggccaa
    14341 gggtatctga aatccctgct ggcgctgggc ttccaagcct ttttaatcat ggttgtggtg
    14401 ggtatctacg cggtgctgat acagaatatc ggttccgctt ccgacatatc cggcgcaatc
    14461 tggggctgta tgggctatac ggtgctgctg tgcttctgcc tgttcaagac cggcagtata
    14521 tcaaaggccg tattcggggc gcattgatcc caaaatccaa gcatacacag gaggaacttg
    14581 tttatttcaa cgtacaacat catttacgct gacccgccgt ggcggtatga ctgcaaaaac
    14641 gtacagggcg cagcggaaaa tcactattcc accatgagca ttgacgagct gtgcgccctg
    14701 ccggtggaac ggctggcgtc caaagactgc ctgctgtttt tgtgggccac attccccatg
    14761 ctgccggaag ctctgcggtt aatcaaagcg tggggctttt cattcaagac cgtggctttt
    14821 gtatggctga aacagaaccg taaaagcctt acatggtttt acgggctggg ccgctggaca
    14881 aggggaaacg cggaaatctg cctgctggca acacacggca agccgaagcg tcgttctgcc
    14941 tctgtccatc agtttattat cagcccgatt gaacaacaca gcaaaaagcc ggatgtgacc
    15001 cgtgaaaaaa tcgtagagct ggcgggcgat ttgccccgcg tggagctgtt tgccagacag
    15061 aaaacccccg gctgggacgt gtgggggaac gaggtggaat gtgaccttat cctgacgtga
    15121 aaggaggtgc caaatggctt atgtaaccgt ccccaaggac ttaacccatg tgaaaagcaa
    15181 ggttgtgttt gggcttacca aacggcaact gatctgtttt tccggggcgc tggtgatcgg
    15241 cgtcccctta tattttctca cccgtgacta tcttaccaac agcgcggcgg ccctgctcat
    15301 ggtatttgcc atgctgccgg gctttttgct tgccatgtac gagcgtcacg gccagcccct
    15361 tgaagtggtg attatgcaga tcatccagtg ctgcttcatc cagccaaagg agcgtcccta
    15421 tcagaccgac aacacctaca ccgcccttgt gcggcaatac cagatggaac aggaggtaaa
    15481 ggccattgtc caaaaaggaa atcccaagag aaaccgaaaa aacaaagctg acccgcgccc
    15541 agcgaaaaga aattgacgcg gtaatccgta aatataaggg cgacggcagg ccccacacgg
    15601 cgcagcagag catcccctac gaggtcatgt acccggacgg cgtgtgccgg gtatcgcccg
    15661 gcgtcttttc caagtgcatt gagtttgcgg acatcagcta tcagcttgcc cagccggaca
    15721 cgcagacggc tatctttgaa aaactgtgcg acctgtataa ctatgtggac gcctccattc
    15781 atatccagtt ttctttcctc aatcggaaag ttgacccggt gcagtacgcc aagagctttg
    15841 agatcgcgcc gcagggggac gacttcgacg acatccgggc cgagtacacc ggcattctgc
    15901 aaaagcagct tgcaaacggc aacaacggca tggtaaagac caaatacctg acattcacca
    15961 tcgaggccga aagcgtgaaa gcggcgcgag ccagattgaa gcgcatcggc tttgacctgt
    16021 taggctattt caagagcatg ggggccgtag cccatgtgat ggacggctgg gaacggctga
    16081 acctgctgca tggcgtgtac cacccggacg gcgagatatt caattttgac tggaagtggc
    16141 tggccccgtc cggcctctcc accaaggatt ttattgcccc gtcctccctg tgcttcggca
    16201 atgcaaagac attcggcatg ggcggcaaat acggcgcggt aagtttcctg caaatcctgt
    16261 ccccggagct gtcggacgat atgctggccg acttcctcaa tacggaaagc ggtgttttag
    16321 tcaatctcca tgtgcaggcc attgaacaga ccaaggcgat caagaccatc aagcgcaaaa
    16381 ttaccgacct tgacgctatg aagattgcgg agcagaaaaa ggccgtccgc agcggttacg
    16441 atatggacat tcttcccagc gaccttgcca cctacggcga ggacgccaag aagctgctga
    16501 caaagttgca gacccgcaat gaacggctgt tccagttgac gttccttgtc ctcaacgtgg
    16561 ccgacaccaa gcaaaagctg aataacgacg tgttccaagc cgcaggcgtg gcccaaaagc
    16621 ataactgtcc ccttgtccgg ctggactacc agcaggaaca gggccttgca agcagcctgc
    16681 cgctgggcgt caatcagatc aagatacagc gcagccttac gacttccagc gtggccgtgt
    16741 tcgtcccctt tgtgacgcag gagctttttc agggcggcgc ggccatgtat tacggcatca
    16801 acgccaagtc ccgcaacatg attatgctgg acaggaagca ggcccgttgc cccaatgcct
    16861 tgaagctcgg cacccccggc agcggcaaat ccatgagctg taaatccgag atcgtcagtg
    16921 tgttcttaac gacgcctgac gatattttta tttcagaccc ggaggccgaa tattaccccc
    16981 ttgtcaaacg gctccacgga caggtaatcc ggctgtcgcc taccagcaag gactttgtga
    17041 acccgctgga catcaacctg aattattcgg aggacgataa cccgctggcc ctgaaatccg
    17101 actttgtgct gtcgttctgt gagcttgtca tgggcggcaa gaacgggctg gaagccattg
    17161 aaaagaccgt gatagaccgg gctgtgcgcg tgatctaccg gccctatctg gcagaccccc
    17221 gcccggagaa tatgccgatc ctgtccgacc tccacaaagc gttgttagat cagcatgtcc
    17281 cggaggctga ccgggtggcg caggcccttg acctgtatgt gtccggctcc ctgaacgtat
    17341 ttaaccaccg taccaacgtg gacatcggca accggcttgt gtcctttgac attaaggagc
    17401 tgggcaagca gctcaaaaag ctggggatgc tcattgtcca agaccagata tgggggcgcg
    17461 tcacggccaa ccgaagccaa ggaaaggcca catggtattt tgccgacgag ttccatttgc
    17521 ttttgaaaga ggaacagacg gcggcctatt ctgcggagat ttggaagcgg tttaggaaat
    17581 ggggcggcat tccaacaggt gccacccaga acgtcaaaga cctgctttcc tccccggaaa
    17641 ttgaaaatat ccttgaaaac agcgacttca ttacgctgct caatcaggcg tccggggata
    17701 gaaaaatcct tgcggaacgc ttgaatttgt ccaccgaaca gcagaagtac atcgacaatt
    17761 ccgaacccgg cgagggcctt ttgatctttg aaaacgtggt gctgcccttt accaacccca
    17821 tcccgcacaa cacccagctt tacaagatta tgacgacccg cttaaacgag gtggcgggag
    17881 tatgacccgg cgacgccaca aaggaggtga accaccatga aagagctgaa agccaaggcg
    17941 aaagtgacgc aggccatgac ccgcgacggg ctgacgctgg aaaatcaggc caccggcgag
    18001 gtggagaaca tttccagccg ggaggccgaa caggatttgt ccgcgccggg gccgggcggc
    18061 accgccgata agctgctgga acgggccgac gccatccatg acggccataa ggccaagaaa
    18121 gccgcgaagc aggccggggc tgtggccgcc gagggggacg ctgccctgca ccggccatcc
    18181 gcccggctcc aactgtcgga ggaagaacgg gccgacccgg atttacagcc ctatatccga
    18241 aaatcggaca aacgggccga taagctggat aaggcccgcg ccgccatccc caagaagcgc
    18301 gtcccggtca aggaaaccgt ctttgacgag gcaagcggca aagcaaagac cgtcctgcac
    18361 tttgacaaac aggacaaggg gcctccctcc ctgaaaccca atccggcagg ccgtcctgtt
    18421 tccgaggttt ttctattcgg ccacgggaaa atccatgaag tcgagcatga aaacgtgggt
    18481 gtggagggcg gccataaggg ggaggaactt gccgagcggc aggccggaaa agccatccgc
    18541 agcaccatcc ggcaccacag gctgaaaccc tactgggacg cggaaaaggc cgagcgcagg
    18601 ctattgtctg ccaacgcgga gtatttctac cagaaatctt tgcgggataa cccggagatc
    18661 gcacaggcgg ccagcaaccc catttcccgg ttctggcaga aacagcagat caagcgccag
    18721 tacgccaagg ccgcccggac agcgggccag accgcaggcg cggcggcaca gggcgcggcc
    18781 aagacagccg gggcctccac gaaagcggcc aaggccgcag ccaaagaaag tgaacgggcg
    18841 gcctcctttg tggcccgcca ctggaaaggg gcgctggccg tccttgtgtt ggggcttttg
    18901 atgctgctta tcatcggcgg cttacagtcc tgcaccgcca tgttcggcag cgccggaagc
    18961 ggcatcacgg cgtcctctta cttttccgag gatagcgata tgttggccgc cgaggacgcc
    19021 tacgcggagc tggaagccga gctgcaagac tatctggaca actacgaggc cacccatgac
    19081 tatgacgaat accattttga ccttgacgag atcagccatg acccctatgt gctgatttcc
    19141 attctgtcgg cgctccatga cgggatattt accatcgggg aggtacaggg tgatttgtcc
    19201 atgctctttg acaagcaata tatcctgaca gaaaacgtcg tggtagaaac ccgttaccgc
    19261 accgaaaccg atacatggac ggacgcggag ggcaacaccc atacagacac ctaccaagtg
    19321 ccgtatgact attacatctg ctacgtcacg ctggaaaact tcgacctgtc ccatgtgcca
    19381 gtctatatca tggacgagga aacgctaagt ttctattccc tgtatatgtc cacgctagga
    19441 aaccggcctg atctgttttc cggcagccag taccccaacg cctccaccct gacgccgccg
    19501 acctactacg acgtaccccc ggaggctttg gaggatgcca cctttgccgc catgctcaaa
    19561 gaggcggaaa aatatatcgg ttatccctat gtgtggggcg gcagctctcc cagcaccagc
    19621 tttgattgca gcggcttcat ttcgtgggta atcaatcata gtggatggaa cgtgggacgg
    19681 ctgggggcgc agggcctata taacatctgc acccctgttt ccagttcgca ggccaagccc
    19741 ggcgaccttg tatttttcaa gggaacctac gacacccccg gcgtgtccca cgtcggcctg
    19801 tatgtgggca acaatgtgat gctgcattgc gggaacccga tttcctacac gaaccttact
    19861 tcaaactact ggcaatccca tttttacagt tacgggcgtt taccttaacg ccagaaagga
    19921 gctttcaatt atggcaacca aacttgaccg catcgaaaag gacatccaga aaaccaagtc
    19981 taagatcgcg gagtttcaga aacagcttcg ggagctggaa acgcagaaaa ccgaacagga
    20041 gaatttgcag atcatccagc ttgtgcgcgg catgaacatg aagccggagg aatttgccgc
    20101 gttcttacgc agcggcgcaa tgcaggccgc ccctgccgca accccttacc atgaacagga
    20161 ggacaatgcc catgaagaat aagaccagac ggatttcccg tattctcccc gcacttctgg
    20221 ccgtgatgct ctgcgtcacg gcctttcccg ttaccgccct tgcaggcggc aatgacccgg
    20281 caccggcccc gctgccggag gctacaaccg aacccgctac gggcggtttg gagcctgaaa
    20341 cagacgaaac cggcaccgcc acggacggga cagaaaccga ggccgaaaaa gataccggcc
    20401 ctctgaccgg caaggacatc accgacctgt tttccgcgct gttcggttcc aaggtgagca
    20461 ttgccgccac ggacgacggc atccagatca ccaccggcac cgacaaaaaa gaacctgaac
    20521 agaccggcac cgtcaccacc aacggcggca ggctgaatgt ccgcaccggc gcggggcttg
    20581 acaagaccgc gttcacccag cttcccaatg ggacgacggt tgaggtagtc ggcacagacg
    20641 gcgactggat taaaattctc ttgccggagc gcatcggcta tgtccattcc gactatatga
    20701 ccgtcagcga aaaggaagta gcggctaccg gcgagggcgg tttttccctc tccattgacc
    20761 cggaggaaat tgcttctctg ctggaactgt tcaatggcgg cggggccagc gccgccctgt
    20821 ccccggacgg gaacctgtca ctgattgacg acatcggcag cgccgcgaaa aatggcaagc
    20881 agtttatcac cgtggaaacc aaaaacggca atgtgtttta tctgattatt gaccgggacg
    20941 acgagggaaa agaaactgtc catttcttaa atcaggtgga tgaagccgat ctgctgacgc
    21001 tgatggagga cggggaaacc gtcacccccg ctgctgtctg ctcctgtacc acgaaatgca
    21061 aggccggggc tgtgaacacg aattgcccgg tatgtaaaac caacctgacc gagtgcagcg
    21121 gcccggagcc gcaggaaccc cagccggagg aacccgaagc cccggaggaa gaacccaagg
    21181 gcggcgcggg cggcctgatc gtgttcctgc tggtggcgct ggcgggcggc ggtgcggccc
    21241 tttactattt caagttcaaa aagcccaagg ctgaaaccac cggcagcgac gatttgggcg
    21301 agtatgattt tggggaggac gaggatttgg aggacgagga acccgaaacc gagtttgacc
    21361 cggaagctga ttttgtgagt gaacaggagg acgaggaatg acatataaac ttgtaatcgc
    21421 ggaaaaaccc agcgtggccc gcagcattgc gggcgtgatc ggggccacgg agaaacacga
    21481 cggctatttg gaggggaacg gctatctggt gagctggtgc atcggacatc tggtgtcctt
    21541 tgccgacgcc ggacgctatg acgagcgttt caaaaaatgg cgctatgagg atttgcccat
    21601 tctcccggaa acatggcagt acatcgtccc cgacgacaag aaacagcagt ttgacgttct
    21661 ctgttcgctg atgcagcgcc ccgacgtgac cggccttgtg tgcgccaccg acgccgggcg
    21721 cgagggggaa ttgattttcc gtttcgtcta ccagatggcg ggatgccaaa agccctttga
    21781 gcgcctttgg atttcctcta tggaggacag tgctattaag gacggctttg cccacctgaa
    21841 accgggcggc gactatgatc cgctgtatca atcggccctg tgccgggcca aggccgactg
    21901 gctgatcggc attaacgcca cccggctttt ttccgtcctg taccacaaga acctgactgt
    21961 gggccgcgtc cagacgccga ccttaaaaat gctggtggac agggacgcca agattaccga
    22021 tttcaagaaa gagaaatatc acatcgtcca tatcacggcg ggcggcgcgg acgcggtaag
    22081 cagccgcttt tccgacgctg ccgaggcaaa caccgtcaag gcggcttgtg caaaagcgca
    22141 ggccgtttgc gtttccgtga cgcgggagaa aaagacggaa cagccgccca agctctatga
    22201 ccttaccacc ttgcagcggg aggctaaccg gcttttcggc tataccgcca agcagaccct
    22261 tgactatgcc caggcactgt atctaaagaa gctgctgacc tatccgagaa cggatagtca
    22321 atatcttacc gacgatatgc tgcccgccgt ggaaagcctt gtgtccggcc tgtgggagct
    22381 ggtgccgttt gccaagggcc tgaacctttc cccgcagttt gaccgcatcc tgaacagcaa
    22441 aaaggtgtcc gaccaccacg ccattatccc caccgctgaa tttgtgaagc agggctttgg
    22501 cactctggcc gagagtgaaa aaaagctgct gtccctgatc tgctgcaagc tcttatgtgc
    22561 cgtggccggg ccgcatatct atgaggccgt caccgccaca tttacctgtg ccgggcagga
    22621 gtttaccgcc aagggcaaga ccgtcctttc ccccggctgg aaagagattg aacgccggtt
    22681 ccgttcttcc ttaaagaccg acgccgacga ggacgccgag gcggtgcggg agctgccgga
    22741 gctttgcgag ggccagacct ttgcagacgt ggccgcctct gtcaccgagc atttcaccac
    22801 gccgcccaag ccctacaccg aagatacgct tttgtccgct atggaacggg ccggggccga
    22861 ggatatgccg gaggatgccg agcgcaaggg attaggcaca ccggccaccc gcgccgccat
    22921 tctggaaaag ctggtgcaga tgggctttgt ccagagaaaa ggcaagcagc tcattcccac
    22981 caaggacggc atcaatctgg ctgtggtgct gccggaggcg cttacttctc cccagcttac
    23041 tgccgaatgg gaaaaccggc tgaccgagat cgccaagggg caggccgatc ctgacgaatt
    23101 tatggacggc atcgagacgc aggcgcggga gctggtgcag acctattcct gtatcagtga
    23161 ggaaaagcag aaattgtttc aggccgagcg tgttcctatc gggacgtgtc cccgctgtgg
    23221 tgaggctgtc tatgagggca agaaaaacta ctactgcgga aaccgggcct gccagttcgt
    23281 catgtggaag aatgaccgat tttttgagga acgcaagaaa gcgttcaccc cgaagatcgc
    23341 cgctgcgctg ctcaaaaccg gcaaagtcaa ggtaaagggc ctctattccg taaagaccgg
    23401 caaaacctac gacgggacgg tgcttttggc tgataccggg ggcaagtacg tcaactaccg
    23461 cattgaacac aggagctaaa ggacagtaac acagcaagca tagggagtgt ggctacactc
    23521 ccggtaaaat cccccggccg cgtccctgcc gggccgggga tttttgcttg ttggggaagt
    23581 ccccaacccc ctattttttg aaacagaaca gagaaaggag tgaccgcgtg aacgaagtat
    23641 tttctatcca agtccataac cggcagtttt tggaacaggg caaacaaggc gtctggctgg
    23701 atttgcccac cacaaccgaa acgctgcaag ccgccctgcg ggatgttggc attactgccg
    23761 acaatccgca ggattttttt atcaacgggt attcctgccc ggaggacaga caccttgcca
    23821 ttccctatga catggtgctg gccgccgacg tggacgagct gaatttcctt gccgcccggc
    23881 tgggccagct tgacgccgcc gagatcgcag agctgaacgc cgccctgcaa aatccccgtg
    23941 gcggctttga gaatatcggc cagatcatcg actacaccga aaatgtggat tactatgtgc
    24001 atcttcccga cgttaggggg gatggacagt tgggcgacta ctacctgaac cgttccggca
    24061 tggtggatat gccggaggaa tggaaagccg ggattgatac ggcgcagttt ggacgccaca
    24121 ttgcccagca ggaacaaggc gtttttaccc agtacggcta tctggtgaga agcggcgacg
    24181 aatggcagcg cgtccatgag ggccagccgg tgccggagga ataccgggtg atgcactacc
    24241 cccagccgga agctgaccgg gaggaaccgc aggcccagca gccggaggca tccgaaagcc
    24301 ccgacaaagc gccgcccccg gttatcccga tcatgctgga cggcatgaac agcaaagaac
    24361 gcatgaaagt gattactgac cgtctggaaa ccggcgtaca ggagcttttt gaaagtagcc
    24421 gctatatggc ttacttatcc gctatgtcca agatgcacga ttacagtctg aacaatacgc
    24481 tgctgatcgc catgcagggc ggttcgattg tggcgggcta taacaggtgg caggatgaat
    24541 ttgaccgcca tgtgaagcgg ggcgagaaag gcattaagat actggccccg gccccgttca
    24601 aggttaagca gcagatggag aaaaccgacc cggccaccca gcagcccgtg atcggcgcgg
    24661 acggcaagcc cgtgaccgaa gaacaggaag tgacggttcc ggcctttaag gtggtgacag
    24721 tctttgacat cagccagacc gagggcaagg attttcccat gccctatatc agtgaactta
    24781 ccggcgacgt gcagcagtat caggatttct ttgccgcgct ggaacggaca tcaccctatc
    24841 ccataagcat tgagccgatt tcagacgggg ccaagggccg ctgcttctac gcaaaacagc
    24901 gcattgccat caacgagggc atgagcgaat tgcagaccct aaagaccgcc atccatgaga
    24961 ttgcccatgc caagctccac gccattgacc gggaggcccc cgccgaggaa caggcggccc
    25021 ggccagaccg cagcacccgc gaggtacagg ccgaaagcgt ggcctatacc gtctgccagt
    25081 attacgggct ggacacgtcg gactattctt ttggctatgt ggccgattgg agcagcggca
    25141 gggagttatc cgagctgaaa gcctcccttg aaaccatccg ggccacggcc agcgaattga
    25201 taaccgagat cgacggccat tttgcccagc ttcagcagga gcgcgaggcc cggcaggaac
    25261 aggaggccgt cacggttgac gttaccccgg aggcagcggc cccggcgcag gccgaggaag
    25321 aacaggcccc ggcccttgat ccggcagccg agccgcttgt gactgtgcta tggagcgaaa
    25381 gcccccagct aaaggacggc cagcaaatgc cgttgcatga ggccgacgcg ctgtttgccc
    25441 gtctggatgc agcatatccg gcagaggcag gctatgacaa aaccgcgttc cgcattgact
    25501 gtatctttca gggcgagccg gacagctacg aggggcggca ggattttgga gatagggacg
    25561 gttccctgat cgaacatatc cagagctacc accaatttta cgcgcaggac gaaaactgga
    25621 aaaagcatgt aatcgctaca accggcatag agggctggga gcaggacaag gcccatcggg
    25681 atatgctgtt aggcgagttt gtcccctata tgaagctgca ttgcagcctt gcccaaatgg
    25741 aacagacggc gcagggccgc ttacagtccg gggaaaccct gacgccggag gaaacggcct
    25801 atttgggggc gcttctctct tatgtgcagg agtgccgccc catgctcaat cagggccagt
    25861 atcagttgcc ggagccgccg aaactggctg acttcgacca gagcttgcag gactacaaag
    25921 cacaggtaca ggccgagatc gcgcaggagg ccgccgcagc cggtatgacc gtggaggaat
    25981 acgcggcggc tggctatgaa gcaccccaac aggacacctt ttccatctac cagcttcgag
    26041 gcggggagga aacccgcgac tatcggtttg agccatacga gcgtttacag gccgcagggc
    26101 tggccgtgga cagtgccaac tatgaccttg tttatacggc accgctggat aaaagcacgt
    26161 ctttggagga tatttaccgc acattcaaca tcgaccaccc ggcagatttt aggggacaca
    26221 gcctttccgt ttccgacgtg gtggtgctgc accagaatgg acaggacacc gcccactatg
    26281 tagacagctt cggctatatg gaagtgccgg agtttttgcg ggaaaatccc ctgcgtaccg
    26341 ccgaactgtc tacggagcag aacgaaaaca tgattgacgg cattatcaac aatacgccgc
    26401 ccgaaccgca gccggaggaa cccgaacagc tatcttttga ggatgtggca gttgcttccg
    26461 gccagacaca ggaggaaccc ccggaggctg cgcccccgca aacggctgtc cgctattatc
    26521 ccatcaatga gggagccgcc cgccgcgcca aggaagccat gagcttttct gactaccggc
    26581 ccggcagcgc cacggcggaa taccggcact atgtcgatca ggccgtggaa attgcggagc
    26641 gccagaaaaa gcgtgtggag ccagagtatc acgaaaagat agacagcctc cttgacacct
    26701 acgcccgcaa gctggccgag aacatgaata agggctatga gattaccgcc cgcgtcccct
    26761 ctgtcctgat tgccgggccg tccaatttcc cggtacgggc gaaagaaaag cagaacgccg
    26821 ccagtgaccg caacatggag gaatttcagt acattcaggg cttgcttgac aaaatccgca
    26881 gcaccggcat gggtggtatc agcgccgacg atccgcaggc cgtttccaag ctggaaaaga
    26941 aactggaaaa gctggaagcg tcgcaggagt tgatgaaggc cgtcaatgcc tactaccgca
    27001 agcatgggac gctggacgga tgcccccatc tgatcgaaag aggcattgaa aacctgaaag
    27061 cggatatggc gtcgggctgg cactatgaga aaaaaccgtt ccagtcctgg cagctctcca
    27121 acaataacgc cgagatacgc cggttaaagg gccgcattga ggaattgacc cgccaaaagg
    27181 aggcggccta tgtgggatgg gagtttgacg gcggcactgt ggagatcaac cgggtggcca
    27241 accgcctgca aatcttcttt gaggggaagc cggatgctgc tgtacgcgac gagctgaaaa
    27301 gcaacggttt ccgctggtcg cccaaggccg gggcatggca gcggcagtta aacgacacca
    27361 caatccgcgt cgctgaccgt atcaagtgca tccagcccct ttccggcgaa aagcccagcg
    27421 ccttacagct tgcggcccgc agggagaaag aaaagccctc tatccgggcg cagctcaacg
    27481 cggccaagac cgaacaggcc aagcagccac cggcgcggga aaaatcaaag ggattggagg
    27541 tagagtaaat gcagaagttt gaaaatgtgg acgttctggc cgcgctggaa cagatcatgc
    27601 ggcagaacac cgcgttttat caaagtgact ttggcattga caaagacatt atcaggcggg
    27661 atgccgccag cgatcaggcc gcagacaaga ccctgctctg gatgtcccgg ccctccggga
    27721 cgtactgttt ccgggaacgt gatgttttcc tgaaagacac ccgacagtac aacacatgga
    27781 aattttacgg ggagcagacc cgtgacaaga tactggccta cgcggtggag ctgaccggca
    27841 cacagggcgg cactatccgg ggcaatctct atgagctgga ctatccgcag catttccggc
    27901 acgtcattga aaccgcccag ccggtgagtg tgaaccggct cttttatgag catggcaccc
    27961 gtgacatccc ggagagccag cgttttgacg gaagccctga ccgggtattg ggaaactttt
    28021 tgtactatga ggcccagccc catgacccgg ctgtgctgca ggaggcgcta cggacggagc
    28081 agcacagccg ggggcgggcc gtccccggcg atttcaaagt ccacgtcacc gctttgcatg
    28141 acagccggat tgaaaccgag gcccggcgta ttgtggacag cctgaaagat ctgtccgcgc
    28201 ctaacagccc caataaaacg catttcatgg tggagatctc cccgcacttt gcggcgattg
    28261 cttccaccaa ggataatgac cggctctttg ccatgctgcc ctataaatcc ctgtgtttta
    28321 ccgggatgaa aggcaggcat ggcgtgtatg ccgtgattaa ccgggacgag cgccgggatg
    28381 tgagtattcg caggccccgg ccctctatcc gcaagcagct atccgacagc aaacaggccg
    28441 ccagcccaaa aaaggcggcg gccagaacca aaaagaatga attggaggta taaggaatga
    28501 tgcactttac cgtggaggaa gaaaacctga tctgtatgta ccacaatgca gaccggcgca
    28561 gaacgataaa caggctcacc gccgtcatgc cggttatgga tggggatatg cgggcgcagg
    28621 cccagcagac cctttccaag ctggaacgca tgacggatgc cgactatgac ggccagaagt
    28681 tccacttcac agacgaataa cgaacagcag acgccgggcg cggcggccat gtggtcgctt
    28741 gcgtccggcg tcttttcttt ttgcctattc ctacgcattt agaacccggt attgtcgggg
    28801 ataggatgaa gtattcgctt gcccctgcaa acatgcggga gaagccttgc agcgggcggt
    28861 tttcaatccc ttaaatgcgt aggttgcagc tataattaga cccctgaaac agatatagcc
    28921 gcgcagcggg tgtatctgtt tcaggggtgt gcaggtgata gccgcaaagc ggcgtaagag
    28981 ggtgcagccc tcttgccgtc tatctctgtt ccagcgggaa gctcggcaat gcttccagca
    29041 gtttcaggga aatgcgctgc cgtatatctt cgtcaatgac tttcttcttt ttgccgtttg
    29101 cctgtctgat ctccaccgtg gaaagctcgt tgatgtagtc agcataatgg gccaacactt
    29161 tttccgtggc ccacctctca ccggcaacgg cggctttgat cgtttcatag tccagcagct
    29221 tttcttgtcc gtccatcctg atacccccgt cagactgatt ttttctatga taccatattc
    29281 ggaagcatgg aaccagagaa aacggcgctt ttctgctggt aaattctacg catttagaac
    29341 agcacttcgg cggggatagg ataaaacacc cacatacccc gctgaccatg ccggaaaagc
    29401 cttgaaatat aagggctttt acagctctaa gtgcgtaggt ttccagtatc ttttccgctg
    29461 gcgctcggcc tctttgcggc ggcgcatccg tttggagcag gccgcgcagt atttggcccg
    29521 gttagacttc ggcgtgaacg tggccccgca ctcacagcag cgtttcactt cgtcccggtc
    29581 tttggtgatc tcggcataca gggccgcatc cagcgggagg acagcggcct taaaccagcg
    29641 gcacagaagc gaataggaaa tgctctgtac gcaaatacat tcttcgccgt cgtccagcaa
    29701 caggcagttg ccgtcacaat agttgcagca ctcatgcgtc agcttccggg ccttgcgata
    29761 ttgagggtaa ttcattcgtg gaatactcat agctcttgcg tccattcctt tcccggcgtc
    29821 atacgcaaaa tgccgtccac attctgcttg atgatgccgt attcctgcat ctgctttttc
    29881 gcttcgccgt acttctcata cagccgttcc ttttcctcgg ccaatcgctt gtactcggct
    29941 ttcagggcgt acagatcggg gagctttttc acaccctgtc cctttagggc ctttgccgca
    30001 gcttcataca aaatcaacgt gctttcatgc tcacgccgga attttgcctt atccgctgca
    30061 ttgcggtatt ccatagccac gggccagagc tgcttgtagg tagaaatatt ctttatcagc
    30121 accgccatat ccgcaagccg gtgttccata gctttgaggg tagcggcggc ctcgtcgctg
    30181 gcggcggtga tctcggccac tttgctttcc aaatccgggt aggtgtctat cccatgttca
    30241 gtcaggaaat tcaaagtctt tgcggcctgc ttcaaattgt ggagcttcgc ccacctctca
    30301 tagcctgccg actgctgggc cttaatgttg ttttccagat caatccgcaa agaaatcccc
    30361 ttgcgctctt tgggcgctct ggtgataacg cggcctttga tacgctcttt gatggcttcc
    30421 tcggtgtagg cttcccccaa tgtcttgcag cgggtaaagc gttcctgtcc gggagcgcgg
    30481 aaagaaataa actttcccgg cttgatctca tagccctgcg cctgcatgag ccgcaggaaa
    30541 tcatcaaaat cttttgcctg cgggatagcg gcgtcaatgg ctgctttgag cttcgctttc
    30601 cagctcgtcc ctttccgctg ggcgtcccac tcggcatagc tcttgccctt actctggccg
    30661 ggcttcacca cggacaatcc gttttccttg cacagcctgt cgctggtgcg ccggatatag
    30721 gcatagctct gtttgttgga aatgtacttg cgctgattta ccatatccac ggcacagaaa
    30781 ataatgtgat tgtggacgtg gcccttatca atatgggtag tcagcacata gggatatttc
    30841 ccttgcagca cttcctcggc aagctgcttc ccgatctcat gggcctgttc cggcgtggct
    30901 tcgccggggg caaaggattg tatcaggtgg tggcccagat tattgccctt ttggtatgcc
    30961 tgcgccagca gcatttcaaa ttcaatgtcc gctgtttcat aagagcaggc aaaggaggcc
    31021 accagcagct tgccgtcagt tttgtccggg ttctcgatgt agtcaagggc ttttttcagc
    31081 gttgacttga taggatgtat ctttgtaact gccatatctg cgccagcgcc ccctttatat
    31141 cctcgatgtc ctgtgcgtac accggcccca taccgttcag cctctttgca atctgattga
    31201 cgttgacgcc gatcttctgt atctcggcgg tctgcgcccg gatgtcgctg gtgtccatgt
    31261 tgacgatata gccgtcaatg agcatcttcc gggcgtaggc tgaaaagttc ttcgtatgca
    31321 gcagcgccat tttctgttca atcatagccc gttcctccgg cgtcacaggg acgcgcagca
    31381 cgatttttcg ctttctgttt gccatgcttc accgtccttt ccattcaggg gtttggggat
    31441 ttccccaaca agcaaaacgg acgcggcccg gcagggacgg ggcaggccgt ttttccggga
    31501 gtggctacac tccctatgct tgctacgtta ctgtttctcc actaacaaga cgtttccaaa
    31561 gccccaaact acccgcaaaa tagccctctg gaaagaaaaa agtgcaaaaa aatagaagct
    31621 acgctgtgca gctcccgcta aaagtaaaac agaggatgcc agttacacat cctccgtttc
    31681 attcttcttc ggcttttcaa ttagtatggc gctgtcaatc gcgccctcga tgatcggcag
    31741 gtattcttcg gggcagagtt tcagcttgtg gctgaccctt tgccgcagtt cgctttcctc
    31801 ccgcatgatc tccgggttaa agtagcgttc cacaggaagc ccgcaggctt taataagctg
    31861 tatgacaacc ggcagactgg gaatggcccg tcgttttcga tgttggcaag atagcgccag
    31921 tcaatattta ttttctctgc cattgctttg cgcgaaacgc gctttgcttg ccgggccgct
    31981 ttcacatccg cgccgaaagt ttcaaagccg ggacaatctt caatattcgc catatcgcac
    32041 ctcccactta cattgtatat ttcatactct gcccgtggaa tgttggtata tgcacctttc
    32101 ttaaagttta tatttcacat tttgtttctt ttattcttcg agcaaaagaa ttataatgta
    32161 ccctgtggag gtgtactatg gactacatga cgttgaaaga ggcaggcgag aaatggggtg
    32221 tgcccccacg atggataaac tactattgtg ctgcgggacg catccccggc gctgtaaaga
    32281 tggcgacgat ctggctcatt cccaaaactg cggaaaagcc gattgacagg cgtacaaaga
    32341 aaggaaaggc ggttactgaa tagtataatg aaaccggcgc attttggata tggttttgaa
    32401 gagagagaaa gcatggtatt ttgtcacgga aaaactataa gatgatagat ggcaggctgc
    32461 tccagaccga taaaaagtat tctcatctga aacagaaaga aaagatttac acatggatgt
    32521 atgaagaagc gaaactttat catgacagaa atggaaaatg cccgaaacaa agacgaggaa
    32581 gctgctgctg tatcagccgt atatggtaga attgaaaagg ccggcatctg gattctttat
    32641 ggtgaagtct ccagacattg gataactgct ggtctaatgc gcctttgagt atggggtgat
    32701 tgattttaat accggggagg tacggctttt tgaggaaaat attctgggat ttacatttgg
    32761 cgattattac atttcctgta actgcaagga cacggatgat gaagatatga ggaatataga
    32821 cttgctgatt ttctactgtc ttgaacggta ttaggactga atctgctgcg cggagtgcgg
    32881 caagagcgac cggagggagt acacacaggc cctgcctgtg tgaaaagcca taccgcaagg
    32941 tgcattcgca aaaaagaggc cgcgcatttt gctgaggctt ggggtgtccc caacaagcaa
    33001 aacgagaacg ccgcaggcgg gcaagtggtg gatcagaata tccgtaaagg atattctgat
    33061 ccattgcttg ccagtattat cgggatgttt tgacaaccgc tgtaatcgaa acatcccaaa
    33121 cattaagaag gtgttaatta ttattatctt gatttaattc gtttaaacca ataatataga
    33181 tacatacacc atgaaaagaa gcgagtatcc caatgacgga tgattaaaat ccgaaaagaa
    33241 atcgctttga aactacaggg aaactacaga ctgttatgtt atcttcttaa aatggaggga
    33301 tttttatgtc gatacgaatt ctacttgtcg aggatgatga tcatatctgt aatacagtaa
    33361 gggcgtttct ggctgaggca ggatatcagg tggatgcctg cacagatgga aatgaggcat
    33421 acaccaagtt ttacgaaaac acttatcaac tggttattct tgatattatg ctgcccggta
    33481 tgaacgggca tgaacttttg cgtgaatttc gtgcgaaaaa tgatactccc attctgatga
    33541 tgacagccct gtcggatgac gaaaaccaaa tccgggcgtt tgatgcagag gcagacgact
    33601 atgtaacaaa gccattcaag atgcagattt tactaaagcg ggtggaagcc ctgctgcggc
    33661 gcagcggtgc gctggcaaag gaaatccgtg tcggcaggct gacacttctg ccggaggatt
    33721 tcacggtact ttgtgacggt acagagctgc ccctgacacg aaaagaattt gaaatccttt
    33781 tgctgctggt gcagaacaaa ggcagaacct taacgcatga aatcattttg tcacgtatat
    33841 ggggatatga ctttgagggt gatggcagca cagtccacac tcatatcaaa aatttgcggg
    33901 ccaagctgcc ggaaaatatc atcaaaacca ttcgcggtgt aggataccga ttggaggaat
    33961 cataataatg gaaagaaaag ggattttcat taaggttttt tcctatacga tcattgtcct
    34021 gctactgctt gtcggtgtaa cagcaacact gtttgcacag caatttgtgt cttatttcag
    34081 agtgatggaa ttacagcaaa cagtaaaatc ctatcagcca ttggtggaac tgattcagaa
    34141 tagcgatagg cttgatattc aagaggtggc agggctgttt cactacaata accaatcctt
    34201 tgagttttat attgaagata aagagggaag cgtactctat gccacaccaa atgccaatac
    34261 atcaaatagt tttagaccca actttcttta tgtggtacat agagatgata atatttcgat
    34321 tgttgctcaa agcaaggcag gtgtgggatt gctttatcaa gggctgacaa ttcggggaat
    34381 tgttatgatt gcgataatgg ttgtattcag ccttttatgc gcgtatatct ttgcgcggca
    34441 aatgacaacg ccgatcaaag ccttagcgga cagtgcgaat aaaatggcaa acctgaaaga
    34501 tgtaccgccg ccgctggagc gaaaggatga gcttggcgca ctggctcatg atatgcattc
    34561 catgtatgtc aggctgaaag aaaccatcgc aaggctggag gatgaaatcg caagggaaca
    34621 tgagttggag gaaacacagc gatatttctt tgcggcagct tctcatgagc taaaaacgcc
    34681 catcgcggct acaagcgttc tgttggaggg aatgcttgaa aatatcggtg actacaaaga
    34741 tcattctaag tatctgcgcg aatgcatcaa aatgatggat aggcagggca aaatcatttc
    34801 cgaaatactg gagcttgtca gcctgaatga tgggagaatc gtacccatag ctgaaccgtt
    34861 ggacataggg cgcacggttg ccgagttgct gcccgatttt caaaccttgg cagaggcaaa
    34921 caaccagcgg ttcgtcacag atattccagc cgggcaaatt gtcctgtccg atccgaggct
    34981 gctccaaaag gcactatcca atgtcatatt gaatgcagtt cagaacacgc cgcagggagg
    35041 cgaggtacgg atatggagtg agcctggtgc tgaaaaatgc cgcctttttg ttttgaacat
    35101 gggcgttcac attgatgata ctgcgcttcc aaggctgttc accccattct atcgcattga
    35161 tcaggcgcga agcagaaaaa gtgggcgaag cggtttagga cttgccatcg tacaaaaaac
    35221 gctggatgcc atgagcctcc agtatgcgct ggaaaacacc tcggatggcg ttttgttctg
    35281 gctggattta ccgctcacat caacattgta aatatttaaa acttaaatga ttt
//
LOCUS       DACCYH010000022        34960 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.022, whole genome shotgun sequence.
ACCESSION   DACCYH010000022 DACCYH010000000
VERSION     DACCYH010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.022"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            156..1862
                     /locus_tag="IVY85_001016"
     CDS             156..1862
                     /locus_tag="IVY85_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP8044045.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="IVY85_001017"
     CDS             complement(2020..2922)
                     /locus_tag="IVY85_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8044046.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3623)
                     /locus_tag="IVY85_001018"
     CDS             complement(2919..3623)
                     /locus_tag="IVY85_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP8044047.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="IVY85_001019"
     CDS             complement(3654..4295)
                     /locus_tag="IVY85_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8044048.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="IVY85_001020"
     CDS             complement(4333..4647)
                     /locus_tag="IVY85_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044049.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /locus_tag="IVY85_001021"
     CDS             complement(4635..5264)
                     /locus_tag="IVY85_001021"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP8044050.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="IVY85_001022"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="IVY85_001022"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP8044051.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="IVY85_001023"
     CDS             complement(5984..6916)
                     /locus_tag="IVY85_001023"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP8044052.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="IVY85_001024"
     CDS             complement(6913..7689)
                     /locus_tag="IVY85_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP8044053.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="IVY85_001025"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="IVY85_001025"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP8044054.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="IVY85_001026"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="IVY85_001026"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP8044055.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="IVY85_001027"
     CDS             complement(12014..13294)
                     /locus_tag="IVY85_001027"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP8044056.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="IVY85_001028"
     CDS             complement(13313..14239)
                     /locus_tag="IVY85_001028"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP8044057.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="IVY85_001029"
     CDS             complement(14252..15535)
                     /locus_tag="IVY85_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP8044058.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="IVY85_001030"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="IVY85_001030"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP8044059.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="IVY85_001031"
     CDS             complement(16323..17234)
                     /locus_tag="IVY85_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8044060.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /locus_tag="IVY85_001032"
     CDS             complement(17231..17695)
                     /locus_tag="IVY85_001032"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP8044061.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="IVY85_001033"
     CDS             complement(17700..18398)
                     /locus_tag="IVY85_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8044062.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="IVY85_001034"
     CDS             complement(18385..18771)
                     /locus_tag="IVY85_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044063.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="IVY85_001035"
     CDS             complement(18918..20588)
                     /locus_tag="IVY85_001035"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP8044064.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="IVY85_001036"
     CDS             20771..21004
                     /locus_tag="IVY85_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP8044065.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="IVY85_001037"
     CDS             complement(21103..21534)
                     /locus_tag="IVY85_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP8044066.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="IVY85_001038"
     CDS             21613..22026
                     /locus_tag="IVY85_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP8044067.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="IVY85_001039"
     CDS             complement(22064..23212)
                     /locus_tag="IVY85_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8044068.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="IVY85_001040"
     CDS             complement(23334..23930)
                     /locus_tag="IVY85_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8044069.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="IVY85_001041"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="IVY85_001041"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP8044070.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="IVY85_001042"
     CDS             27160..27591
                     /locus_tag="IVY85_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8044071.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="IVY85_001043"
     CDS             complement(27636..28985)
                     /locus_tag="IVY85_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8044072.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="IVY85_001044"
     CDS             complement(29055..30218)
                     /locus_tag="IVY85_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044073.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="IVY85_001045"
     CDS             30347..30670
                     /locus_tag="IVY85_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044074.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="IVY85_001046"
     CDS             complement(30853..32202)
                     /locus_tag="IVY85_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8044075.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="IVY85_001047"
     CDS             complement(32271..33224)
                     /locus_tag="IVY85_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP8044076.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="IVY85_001048"
     CDS             complement(33326..34516)
                     /locus_tag="IVY85_001048"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP8044077.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9951 a         7641 c         6120 g        11248 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact
    34681 tatatagtat gaacaattac cttttagata atgatctatt ttactctacc tgggcagata
    34741 aatataacac aaccattgat agaagccctt tcgttaaaat caattgtatt ctgaatattt
    34801 aatttattta ttgcatagac caataaaaat acataaaatt tccatcttcg tttatcagca
    34861 atttttaatt ttactctctg aatatttctt aaaagtttta cttgttgaat tcgaaaaaag
    34921 gactgtgaca aaagtcttgt cacagtccta ttcttccgaa
//
LOCUS       DACCYH010000023        34422 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.023, whole genome shotgun sequence.
ACCESSION   DACCYH010000023 DACCYH010000000
VERSION     DACCYH010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.023"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            352..1041
                     /locus_tag="IVY85_001049"
     CDS             352..1041
                     /locus_tag="IVY85_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8044078.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="IVY85_001050"
     CDS             1028..2236
                     /locus_tag="IVY85_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8044079.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="IVY85_001051"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="IVY85_001051"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP8044080.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="IVY85_001052"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="IVY85_001052"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP8044081.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="IVY85_001053"
     CDS             complement(6339..7034)
                     /locus_tag="IVY85_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP8044082.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="IVY85_001054"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="IVY85_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP8044083.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="IVY85_001055"
     CDS             complement(8233..8424)
                     /locus_tag="IVY85_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP8044084.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="IVY85_001056"
     CDS             complement(8445..9335)
                     /locus_tag="IVY85_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP8044085.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="IVY85_001057"
     CDS             complement(9322..10089)
                     /locus_tag="IVY85_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8044086.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="IVY85_001058"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="IVY85_001058"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP8044087.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="IVY85_001059"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="IVY85_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP8044088.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="IVY85_001060"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="IVY85_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP8044089.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14705..15043
                     /locus_tag="IVY85_001061"
     CDS             14705..15043
                     /locus_tag="IVY85_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044090.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15211..15969)
                     /locus_tag="IVY85_001062"
     CDS             complement(15211..15969)
                     /locus_tag="IVY85_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8044091.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="IVY85_001063"
     CDS             complement(15987..16805)
                     /locus_tag="IVY85_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8044092.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="IVY85_001064"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="IVY85_001064"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP8044093.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="IVY85_001065"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="IVY85_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP8044094.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="IVY85_001066"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="IVY85_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP8044095.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="IVY85_001067"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="IVY85_001067"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP8044096.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20824..21066
                     /gene="yaaA"
                     /locus_tag="IVY85_001068"
     CDS             20824..21066
                     /gene="yaaA"
                     /locus_tag="IVY85_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP8044097.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21053..22177
                     /gene="recF"
                     /locus_tag="IVY85_001069"
     CDS             21053..22177
                     /gene="recF"
                     /locus_tag="IVY85_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP8044098.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22174..24120
                     /gene="gyrB"
                     /locus_tag="IVY85_001070"
     CDS             22174..24120
                     /gene="gyrB"
                     /locus_tag="IVY85_001070"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP8044099.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24140..26611
                     /gene="gyrA"
                     /locus_tag="IVY85_001071"
     CDS             24140..26611
                     /gene="gyrA"
                     /locus_tag="IVY85_001071"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP8044100.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26847..27146
                     /locus_tag="IVY85_001072"
     CDS             26847..27146
                     /locus_tag="IVY85_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP8044101.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27196..27708
                     /gene="ssb"
                     /locus_tag="IVY85_001073"
     CDS             27196..27708
                     /gene="ssb"
                     /locus_tag="IVY85_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8044102.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27734..27970
                     /gene="rpsR"
                     /locus_tag="IVY85_001074"
     CDS             27734..27970
                     /gene="rpsR"
                     /locus_tag="IVY85_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP8044103.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28113..30086
                     /locus_tag="IVY85_001075"
     CDS             28113..30086
                     /locus_tag="IVY85_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP8044104.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30092..30544
                     /gene="rplI"
                     /locus_tag="IVY85_001076"
     CDS             30092..30544
                     /gene="rplI"
                     /locus_tag="IVY85_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP8044105.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30818..32185
                     /gene="dnaB"
                     /locus_tag="IVY85_001077"
     CDS             30818..32185
                     /gene="dnaB"
                     /locus_tag="IVY85_001077"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP8044106.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32965..34257
                     /locus_tag="IVY85_001078"
     CDS             32965..34257
                     /locus_tag="IVY85_001078"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP8044107.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10505 a         6693 c         6817 g        10407 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20701 gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg
    20761 ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg
    20821 gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa
    20881 gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg
    20941 ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt
    21001 gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct
    21061 gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa
    21121 gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat
    21181 ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg
    21241 gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt
    21301 agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa
    21361 gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt
    21421 agtaaaaggc agcccgcaga tcaggcggaa gtttttagat atggaattag gccagatcaa
    21481 tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta
    21541 cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga
    21601 acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt
    21661 ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat
    21721 cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca
    21781 atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt
    21841 tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg
    21901 atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat
    21961 taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga
    22021 tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac
    22081 aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt
    22141 tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa
    22201 cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt
    22261 aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg
    22321 gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg
    22381 attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat
    22441 atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga
    22501 ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct
    22561 gttgtaaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac
    22621 tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat
    22681 cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag
    22741 tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg
    22801 cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac
    22861 ggcgggatca aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa
    22921 cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca
    22981 gacggctacc attccaatat catgagtttt gccaacaata tccatacgta tgaaggtgga
    23041 acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa
    23101 caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg
    23161 acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa
    23221 ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa
    23281 ttcttgctgg aaaacccaag tgtgggccgg caaattgttg aaaaaggatt actagcttcc
    23341 agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag
    23401 attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa
    23461 ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag
    23521 tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac
    23581 aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga
    23641 gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat
    23701 gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct
    23761 attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg
    23821 aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt
    23881 gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg
    23941 gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc
    24001 agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta
    24061 gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa
    24121 tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct
    24181 aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc
    24241 acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa
    24301 cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt
    24361 catgggtaaa taccatcctc atggagacat tgcgatctat gagtcaatgg tacggatggc
    24421 acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga
    24481 cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga
    24541 aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga
    24601 aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg
    24661 gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc
    24721 tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc
    24781 tggaccagat tttccaacag gtggtttggt tatggggaaa tcaggaatcc gaagagcgta
    24841 tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa
    24901 tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat
    24961 cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga
    25021 cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc
    25081 cgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat
    25141 gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaatcc ttgaaaacta
    25201 tatcgaacat caacgcgaag tcatcacacg acggacggtc tttgaaaaag aaaaagcaga
    25261 agcaagagca cacatcttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat
    25321 tgcgattatc cgcgggtcta aatcagatga cgaggcaaaa gcaacaatga tcgaacggtt
    25381 cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg
    25441 tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt
    25501 aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga
    25561 cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag
    25621 tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg
    25681 ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt
    25741 ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca
    25801 tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat
    25861 tccagaatac gggcgtacag ccaaaggaat cccaatcatc aatttactag gtatcgattc
    25921 gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct
    25981 cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat
    26041 ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct
    26101 actgacagat ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt
    26161 caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggtcagag ggatacgcct
    26221 gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaaaatcaag aagtattagt
    26281 gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg
    26341 cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt
    26401 gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg
    26461 tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa
    26521 gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca
    26581 aacagttgaa gaacaaggat cagaagaata accatcaaga ctaagtgtgt aagctagcaa
    26641 gaataaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc
    26701 ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatta
    26761 gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc
    26821 gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta
    26881 ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct
    26941 tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt
    27001 atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat
    27061 cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc
    27121 acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga
    27181 gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca
    27241 gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt
    27301 aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc
    27361 aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga
    27421 cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta
    27481 gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc
    27541 ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat
    27601 caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc
    27661 ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac gaatttgata
    27721 ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta
    27781 cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt
    27841 tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca
    27901 acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag
    27961 cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc
    28021 tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta
    28081 aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc
    28141 cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac
    28201 catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt
    28261 ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga
    28321 ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt
    28381 atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc
    28441 aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga
    28501 aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg
    28561 ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc
    28621 agccggtgat tggtatttta tctattgata actatgcaga tgccattgat aaattagatg
    28681 acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg
    28741 aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga
    28801 tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag
    28861 ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc
    28921 tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg
    28981 accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat
    29041 ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc
    29101 ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg
    29161 ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca
    29221 tcgttatcaa tgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata
    29281 agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag
    29341 aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc
    29401 tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc
    29461 caagtaaacc tctgctgact tatattgaat cttcagcttc ttcagcttca gagctagttt
    29521 ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat
    29581 tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa
    29641 cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt
    29701 tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact
    29761 atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta
    29821 tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca
    29881 ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg
    29941 tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac
    30001 tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata
    30061 tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa
    30121 aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct
    30181 aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca
    30241 aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga
    30301 attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg
    30361 tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat
    30421 caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa
    30481 cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga
    30541 ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa
    30601 ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca
    30661 gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata
    30721 aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct
    30781 tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg
    30841 aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc
    30901 agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca
    30961 tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt
    31021 tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta
    31081 tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat
    31141 cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa
    31201 aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt
    31261 ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc
    31321 cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac
    31381 tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct
    31441 tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg
    31501 aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt
    31561 aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct
    31621 ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat
    31681 ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact
    31741 agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg
    31801 taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact
    31861 ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca
    31921 gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga
    31981 tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga
    32041 cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg
    32101 aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc
    32161 gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc
    32221 tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga
    32281 tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt
    32341 caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac
    32401 gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg
    32461 tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt
    32521 tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac
    32581 ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct
    32641 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc
    32701 acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa
    32761 gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt
    32821 tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat
    32881 aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta
    32941 aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc
    33001 gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg
    33061 taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg
    33121 catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt
    33181 gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa
    33241 acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg
    33301 gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc
    33361 ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat
    33421 aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta
    33481 aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac
    33541 ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat
    33601 gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga
    33661 acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt
    33721 gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc
    33781 ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta
    33841 ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta
    33901 gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat
    33961 gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta
    34021 atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa
    34081 ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac
    34141 gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct
    34201 gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga
    34261 taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac
    34321 gataataata acttgaagag gtagtgaaag agacgtttag ccttattgcc gataggctgt
    34381 ctcttgaaca ctgtttattc agaataaggg actgtgacaa aa
//
LOCUS       DACCYH010000024        33492 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.024, whole genome shotgun sequence.
ACCESSION   DACCYH010000024 DACCYH010000000
VERSION     DACCYH010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.024"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(13..693)
                     /locus_tag="IVY85_001079"
     CDS             complement(13..693)
                     /locus_tag="IVY85_001079"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP8044108.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(742..1377)
                     /locus_tag="IVY85_001080"
     CDS             complement(742..1377)
                     /locus_tag="IVY85_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8044109.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(1443..2846)
                     /locus_tag="IVY85_001081"
     CDS             complement(1443..2846)
                     /locus_tag="IVY85_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP8044110.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(2833..3864)
                     /locus_tag="IVY85_001082"
     CDS             complement(2833..3864)
                     /locus_tag="IVY85_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044111.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            3895..4116
                     /locus_tag="IVY85_001083"
     CDS             3895..4116
                     /locus_tag="IVY85_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP8044112.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(4281..5018)
                     /locus_tag="IVY85_001084"
     CDS             complement(4281..5018)
                     /locus_tag="IVY85_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP8044113.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(5171..6511)
                     /locus_tag="IVY85_001085"
     CDS             complement(5171..6511)
                     /locus_tag="IVY85_001085"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP8044114.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(6748..8271)
                     /locus_tag="IVY85_001086"
     CDS             complement(6748..8271)
                     /locus_tag="IVY85_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP8044115.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(8649..9260)
                     /locus_tag="IVY85_001087"
     CDS             complement(8649..9260)
                     /locus_tag="IVY85_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8044116.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(9383..9508)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(9590..10306)
                     /locus_tag="IVY85_001088"
     CDS             complement(9590..10306)
                     /locus_tag="IVY85_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8044117.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(10312..10881)
                     /gene="scpB"
                     /locus_tag="IVY85_001089"
     CDS             complement(10312..10881)
                     /gene="scpB"
                     /locus_tag="IVY85_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP8044118.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(10865..11662)
                     /locus_tag="IVY85_001090"
     CDS             complement(10865..11662)
                     /locus_tag="IVY85_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP8044119.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(11632..12003)
                     /locus_tag="IVY85_001091"
     CDS             complement(11632..12003)
                     /locus_tag="IVY85_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8044120.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(12022..12909)
                     /gene="xerD"
                     /locus_tag="IVY85_001092"
     CDS             complement(12022..12909)
                     /gene="xerD"
                     /locus_tag="IVY85_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP8044121.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(13005..13478)
                     /locus_tag="IVY85_001093"
     CDS             complement(13005..13478)
                     /locus_tag="IVY85_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8044122.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(13583..14446)
                     /locus_tag="IVY85_001094"
     CDS             complement(13583..14446)
                     /locus_tag="IVY85_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8044123.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(14642..16741)
                     /locus_tag="IVY85_001095"
     CDS             complement(14642..16741)
                     /locus_tag="IVY85_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8044124.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(16961..18070)
                     /gene="rpoD"
                     /locus_tag="IVY85_001096"
     CDS             complement(16961..18070)
                     /gene="rpoD"
                     /locus_tag="IVY85_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP8044125.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(18094..19935)
                     /locus_tag="IVY85_001097"
     CDS             complement(18094..19935)
                     /locus_tag="IVY85_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP8044126.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(20117..22510)
                     /locus_tag="IVY85_001098"
     CDS             complement(20117..22510)
                     /locus_tag="IVY85_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8044127.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(22617..23477)
                     /locus_tag="IVY85_001099"
     CDS             complement(22617..23477)
                     /locus_tag="IVY85_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP8044128.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(23536..24057)
                     /locus_tag="IVY85_001100"
     CDS             complement(23536..24057)
                     /locus_tag="IVY85_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP8044129.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            24377..25096
                     /locus_tag="IVY85_001101"
     CDS             24377..25096
                     /locus_tag="IVY85_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8044130.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            25122..27713
                     /gene="mprF"
                     /locus_tag="IVY85_001102"
     CDS             25122..27713
                     /gene="mprF"
                     /locus_tag="IVY85_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8044131.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(27901..29088)
                     /gene="tuf"
                     /locus_tag="IVY85_001103"
     CDS             complement(27901..29088)
                     /gene="tuf"
                     /locus_tag="IVY85_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8044132.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            29456..29812
                     /locus_tag="IVY85_001104"
     CDS             29456..29812
                     /locus_tag="IVY85_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8044133.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(30053..31057)
                     /locus_tag="IVY85_001105"
     CDS             complement(30053..31057)
                     /locus_tag="IVY85_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP8044134.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            31223..32074
                     /locus_tag="IVY85_001106"
     CDS             31223..32074
                     /locus_tag="IVY85_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8044135.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            32091..32336
                     /locus_tag="IVY85_001107"
     CDS             32091..32336
                     /locus_tag="IVY85_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044136.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            32523..33227
                     /locus_tag="IVY85_001108"
     CDS             32523..33227
                     /locus_tag="IVY85_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044137.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         9466 a         6994 c         5806 g        11226 t
ORIGIN      
        1 ttttttctta atttaaaaaa gaagataacc ttttttcaaa acaacagctt ttattgctgc
       61 aacgacttct ggaatcgatt tgccagtcgt atcgatacga acagcatcct ctgcttgctt
      121 cagcggagag acgtcccttg ttgaatcata atggtctctt tcctgtatct ctttcgtcag
      181 tgtttcaaga tcagtcggaa ttcctttttc ttggttctct ttgaaacgtc tttctgcacg
      241 ctcacttacg cttgctacta aaaagatttt cacttctgca ttcggtaata cagctgtccc
      301 gatgtctctt ccgtccatga caacgccacc gcgttcccct atttttcgtt ggacagcaac
      361 taattcttct ctgacttttc catgtgcaga gacttcagaa acagcttttg tgacatctgg
      421 ttgacggatt tctaaagtca catcttttga tgctatgaat acatgctgtc catcttcttg
      481 ttgcgaaaaa gagataggat actttttgat cagcgaaact agcccttctt catctgaaaa
      541 agatacattg ttcgtaatag ctaaataagt tactgcgcga tacatggcac cggtatctgt
      601 ataaatataa ttcaattctt ttgctaatat cttggcaacg gtacttttcc cagatgaagc
      661 aggcccgtcg attgcaatac tgatttgtgt catgatataa ctcctttttc tcagaaaaaa
      721 agccgcaaag cgcggctatt attatttaat tcttaattct tgaccagggt aaagcataaa
      781 gttgtttgga tctaagccat tcaattcaaa caattgatca gtggtgatcc ctgcacgctc
      841 agccacttgt tttggacctt cgcctgcttg aacagtcgtt gttgaaccgg ctgcagctga
      901 tgaagaaggt gttgtttctg ctgcattgct gcttgaatcc cctgcttgat aagttgcctc
      961 acttgaggaa gtcggtgttg tctctgctgc aatactgctt tctgacgtta cacctgtatc
     1021 ttgagacaca gtagattctt ccgcgattgt actagattct actgttgaag tctcagttga
     1081 ctcctctttt gttgtacttg tagaactttc tactactgat gtcgaagttt cagaagaagt
     1141 tgctgctgtt gcattgttgc ttgatccatt tctgatccag aaataagcac ctgctggaat
     1201 agcgatacat aagaccagta aaatcaaaaa tagcgaaaga aataatgtat ttcctttctt
     1261 ttgttgtctc tgagaacttc ttgatgaaac ttcatcatct gtatcgtaga ttggttgttc
     1321 ccatggttct tgtgtttcgt tattttctgt agattgatgt ctatcttttc tgctcactgt
     1381 tttacctcct aaactctatc gtgtgtatta taccatagga aaaagtgtct tgtcctaaaa
     1441 aatcaatgtt ccatgttttt tttaaataaa tttagtaaaa tcgttcccca atgaagttgt
     1501 tcagtcttag aaaaaagctt cctctttggc agaagttctg cttttgctcc atcaatatca
     1561 caacaattct gcggcttttc ggagagtttt tcgccaaaat aagctaagat gaattttctg
     1621 cggcagtttt cttcattaat atagcgaagc atctgctgca ggcggaattc tttttctttt
     1681 tcctgacgtt tgagccgttc cacccaattt tctggttttt cagactgctg gatcagctcc
     1741 atccattttt tttgcagctc atcaaaggga gcttgctctg tggcatattc caaataaagc
     1801 tcaaaacttt gtcgctgttc tctagaaagt tgattaaaga aataatggat acgttcatcc
     1861 ccactttgat aaaaaagaat tgctgcactt tcttcctgat ctctgcctgc tcgaccgatt
     1921 tcttgtacat agttttctag actgtctggc aaatcataat ggaccacata acggatatca
     1981 gatttatcga tccccatacc aaatgcattc gtagcaatga gaaactgcag ctgatttttt
     2041 acaaactgct gttgaagttg tcttctttga gctgcatcaa gaccaccgtg atagtatcca
     2101 actgtaaaac gttccctgaa taaatgatac aatcgttcga cttctttttt tgttgcacaa
     2161 taaatgattg ccgctccttg tgctctttcc atgaagtgtt caagttcttg ttctttttgc
     2221 tgcgttttcc gaacaaaaag cgagatattt tgccgattga ctgattgtct gatctctttt
     2281 ggtggtcgat caaatagtac atgacgtata tctcttgcta ctaaatctgt tgctgtcgcc
     2341 gttagtgcca gtgtgaccgg gtttcctaaa agctttcgga tttttcctaa ctgttgatat
     2401 tctggacgga aatcaactcc ccattgagac acgcaatgag cctcatctac tacatataac
     2461 gcaatatttt gttttttcag atgctctaaa agagaaggat tagtcaacat ttcgggactc
     2521 aaaaacaaaa atttatattg cgatagatga cggaggacgt atcttctttc agattgcgaa
     2581 agtaaactat tgaaagcagc tactcgtttc tcccctcgtt tttgtaattg cgtcacttga
     2641 tcttccatta gagaaagcag cggtgacacg atgatgacca gaccttccat taaatacccc
     2701 gtcaattgat agcaaaggct ttttcccgtt cctgtaggca aaatagcgag agtatcttct
     2761 ccatcaagca acgcctgaat aatttcttct tggccgggac gaaatgaatc aaatccaaaa
     2821 tattttttta attcatttat taacattctg tatcgcttcc tttctcaata gatagatttg
     2881 atacaaacga aattctccat aatctaattg tccatctaca ttccattcgc taaagcgcca
     2941 ctctcgcacg gaatccttat ttggttccaa acgttcgatt gtttctgcag agagaatcca
     3001 ctcgtatgga aaatcatcaa aaaacaaggc ccactcaata agatgatctg ttactgttcc
     3061 tctttttaaa tgacggattg ccatgatctg gtcaattgtc tgaccattca taaacatctg
     3121 cttggtgatc atcatgcttt gattgaaatt ttgctgtaat aatgaatcga tcaaagcgtg
     3181 ccaaagcgaa tctggccgtt tttcgatttg tgctaacaaa aggtggatac atctgctttg
     3241 ttgcagataa accgctgact catcattgtt ttttgccagc tgataaggaa gcagccctgt
     3301 tcgctcattt ccagataatt ggttagctaa aaaatctgct gcttcttctg gtatctccga
     3361 aaacagctgc accaaactct catacgcagt atcaatcaaa atttcacgtg gcaattggct
     3421 ttcactaagc cattttttta gttggaaagt ataaaaagga cttgtctctg ctggaagata
     3481 gtcttgaata cccgttgcca gattagaaat tacttgaaca gcaaatttaa tgagccgcca
     3541 actagtttcc cctgttctgc catagcggtc aaaacgcaaa ccagtatgtt ctatttgatg
     3601 ttcggacaac atatcagccc ctgcaggcgt tagctttgct tcattttcat ttatttcgat
     3661 ccaaccttgc tgcgcaattt tttgcaaaat ttgataaaac ttatcctgct tcaattctgg
     3721 aaaactgtta tggataaaca aaagttcgtg ggtaaatcca taaataagta ctgaagtcgt
     3781 tcgttttccc acaaggagtt ggtaaagaga actaatccgc agcttgtgtc ctgatgaaaa
     3841 tagcgataaa ataaaaacaa tcatattatt ctccttaagt taggatggtt agaaatgtca
     3901 ctactatgta aacttgtacc tgaacgctgt attgcatgcg gtctttgtca aatcgaagcg
     3961 ccagatattt tcgattatga tgaagaagga atcgttttat ttgcccaaga accagaggct
     4021 catcaacagt tcgtgatgcc ggaagaagaa acatatgtaa aaaaagcgta tcaaaaatgt
     4081 ccagttcgag cgattttatt agaaaataaa atataaatat aaacgaaact ttaaaaacgt
     4141 acactaagtt ctctaaactt gaaaataagc cgggatctgt ttctcttgtt ccgtgaaatc
     4201 ccgacttatt ttgaaagatt catttgctat ctatagttaa ttgactagtt ttttcacgga
     4261 ccctatagga cccatttata ttatttcaac tttttcagcc agttttccaa tgtattgacg
     4321 atcacagttt gctggtctgc gttagaaatt tctgcctctc catctccttt ttgctcacca
     4381 tagctgccaa aacctccatg atttccgcca ttgattgata catactctgt tctttccggt
     4441 aaatactgct tagcttcttg ataagcttcc tgattcaata ctttatcttt tgtcgccgtg
     4501 atcgataaaa caggaacagc tgattctgtc aatgttcctt ttttgtcggg ataacttgcc
     4561 atgaagaata ccccttttaa atgtttgtct gaatgattct tattgacata acgacttgcc
     4621 attacgccac ctaatgaatg gccaccgatc acataatcgg aatttggaaa atcttctttg
     4681 accttatccg ctttgtttcc gcccatcact gctagatcaa gtggcatttt gacaatagcg
     4741 actgaaaagc cgttttttgc taaatcttgc gcccaaatac tataactttc tggctctacg
     4801 agagcaccag gataaaaaat aagtaatgga gactttccat gattcttttt ctctccttca
     4861 aaaaataagt aatcttcttt gttttttcct tcttttacca acacttgcgc ttctgacgat
     4921 ggttcgtacg tattttgctg tatatagtaa taaccggctc caccgagtaa tcctaaaatg
     4981 atgactaagc caattagtat tcgtctaaat attttcactt tctcccacct tttctatctg
     5041 agtgacccct ttttcttaat caagtatgat tataccttaa aaaaataaga gagaaatgca
     5101 aactttccag catcaaataa aaaaagacgg aatatccatt tgaatatccg ccctttttgt
     5161 atgtttattt ttatttagca cataaacgat acacagcttc tgcataaatt tccattgctt
     5221 tatacatact acttaattcc catcgttcat tcgcctggtg cataaagtca ggagtatcag
     5281 ggaacatcgc accgaatgcc acacactggt tcatcgtacg agcaaaagta gcgccgcctg
     5341 atacaaacgg ttcagtcatg tctccagtaa tatcgcgata tgtccccaat agatttttca
     5401 ctaactcact gtctagtggc acgtataatg aatcaaggaa gtcaaattct tcataagtca
     5461 acccatattt ttctgctgtt ttagttaatt tagctactaa atcatctttc ttgaatgtaa
     5521 cagggattct catatctaca ccgatttttg tttgttctgg tgtgatttcc aaacttgcaa
     5581 agttcatcgt tagctcgccg gattgttcat cttctgtttt gcccacgaca gcttcaccag
     5641 ttgcattttc ttgaactaat tttcctaaga aattgattgg gccaaaatca aatacttctg
     5701 aaagagcaat tgccagacga gaaatagcat tcactccttg tgctgcatct ttcgcgtgga
     5761 tacttttacc aagaacgacg atgccttcac cttgttcttc gaaatcgaat tcgtgttttt
     5821 tcaatgcttc ttttacttca gaaagctttt ctccagagta aggagctgca tcaggaacaa
     5881 cattcaacgc tcctccagct tttacagaaa actcgttcgt tcctggtcct gtcaagtaag
     5941 cttgcaataa gcctttttct gcataaatta aaggaaattc agcatctggc gcaaatcctt
     6001 gcgtaattcc ttcttctttt tcgttatatt tttctaaaca acgccataaa ttttcttcat
     6061 ctgtaccaaa gataaaacga atacgtgtct tgaattccac gccagcatca atcaaagctt
     6121 tgacggcata cattgccgca atagaaggac ctttgtcatc ttgggatccg cgaccaacta
     6181 accagccgtc ttttatagtt ggatcgaatg gttttgtttc ccaattattt tcgtcccctg
     6241 ctggaacaac gtccatatga cacaagatac cgaataattc atctccagag ccaatctctg
     6301 aataaccgta atagccttct ggatctttaa atgttctaaa tcctagcttt tcactgattt
     6361 ccaatacttt atccaaagct tcaatcactt ttttaccgaa gggatgtcct tgtcctgaat
     6421 ctgcttcatc tagtacggaa ggaattcgga tcaattcgct caatgattct aatgcttgtt
     6481 catgatgttg ttctgtcaca aattttttca tgatattatc ctcctaatat gttaaaaagg
     6541 gtcatgtcaa attcctccga cacaacctct tcttctcaaa taatgacgtt ttaagcctgc
     6601 tccatcagca agcagaatgt tcttaacaaa tgatgaaaga aaaaacactc actgaatgga
     6661 gctattgact tatccacgtg cttatcgata gccgtttatc ggtttcttca ttcttatgat
     6721 agaccttgta cctgtatttg tctaggttta gaacattgct gctacaccta ggatcacaca
     6781 gatggctaca aataatatcg caattaattt accggtaaat ttccaccaca ctgaaacatc
     6841 gaaacgagca attgctacag cacccattac gatagcagat gttggtgtaa ttaagtttac
     6901 taaaccagat gcagattgat aagcagtgat tactaaatcc ttaccgacgc cggcaaattc
     6961 acccataggt cccatgattc ccattgttgc agcagctaag ccggaagttg aaggaatcaa
     7021 gaatgacata gggatataga aaataaatgt aataatgatg aatacacttg aagataatga
     7081 acttaatcct tgttcgcccc aatgtaagat ggtgtctgtg attaatccat tgttcataac
     7141 cacttggata ccacgagcaa cagctactac gattgctaca ccgatcaaat cagaagcacc
     7201 agctaaaaat tcagagacaa attcagactc tttcatgcca taagtgattg ctactacgat
     7261 ggacattact aagaataaca tagtaatttc tgggaagtac cagtcaccta atggaagcat
     7321 gcttttacct agaaccgtac ctagtaccgg aaggtttgtt aaccagtttg tcaatgattc
     7381 aaaaatcgtg aaattcgcat tcaaggttgt ccaaggaatc aaactgatga tcatgatacc
     7441 aaaagtaata aagaacaata cattaacatg tttttgtttt ttcgtgatta cttcttgacg
     7501 accgattgct tcaatatcga aatgcttcat atcttcttta cgttggttgt atactaatga
     7561 ttttgttgga tctttttcga tctttgatgc ataacgatac acatatacaa tagaaatagc
     7621 taacaaaata acgaacatca agaatcgaag accaatacct tcacccatcg aaatcccaac
     7681 agcttgtgaa gctacgcctg ttgcaaaagg atttaccgtt gaagctagac agcctacttg
     7741 agaccctact aatacgattg ctacagctac tactgtatca aagcctacag ccatcatgac
     7801 tgggatcagt aaagcataga atggaatcgt ttcttctgcc attccgtaag tagagccgcc
     7861 taatgcaaat aacagcatca atactggaat aagcattttt tcttttcctt tgaatttctt
     7921 cacaactgaa gcaatcccgg catctagtgc ccctgttttg ttgataatac ctaaaaatcc
     7981 accaacaacc aaaatgaaga aagagattgg gattgcagca ggtgtttctt tagttagctc
     8041 ccccgctggt acacctagca tcccattgat tggcgccatg aaaatatccc acagcccttg
     8101 aggattttgt tgtactcgtt catatgatcc agcgacgatg ttgcctgcat cgtctacact
     8161 atagtgccca gctggtataa accaagtaag tacagcgatt actgcaataa taataaataa
     8221 gaccgtatac gctgaaggca tttgaaattt tttctttttt gtttgttcca tcttcttctt
     8281 cctttctttt ccgttagaga caacaatttt ggctctaatg ttattttatc aaaaagagta
     8341 tcagaacaaa aataataaca cgcattttgt aaaacttttg ttcaaaaagt tcgtttttcc
     8401 ctattccctc ctcttttttc aggtttttaa agctttcttc tttttcattg tagcgtttcc
     8461 ttttttttat aaacaaacat tttcgtcttt taaaaggaga agaatttcac atttgcaaag
     8521 aattgattga tttttatgca taaatcaaaa aaatactcaa aacaaacaaa taaaacgttt
     8581 acattattgc atgtcttgta tttttttcca aaaaaaaaga ccaagaaatt tcttgatctt
     8641 tttgatattt atttgactgc tgtatgacgt tgttcgaact gatgttgttt tcgacttaac
     8701 catggaagaa gttgcgcatg cagcactaaa aagacagcac tcacgatgaa tcctttgatc
     8761 aagttgaaag gaacgatccc aatcagtaca tagtttgcta aagacatgcc cagcatctgt
     8821 gttgcgttca gccctaaaaa cattaagtaa agaggcgtaa taacgaaata atttgcgata
     8881 ctcataaaaa tagttagagc gatggttcct gtaatgacac ctaaaaattt gtttcttcct
     8941 ttatgctgtt tctttacact aaagaaataa taaatcggga atgtaaagat cgttgttgat
     9001 aagaaactag cagtgtctcc taccaaatta tccggagaaa aacctgtagt cagcagatgt
     9061 aatactgaac gcaagaacgc tgttgcaact ccggccgctg gaccgaataa gaacatgctg
     9121 atcaataccg agatatcact aaaatcgatc ttcaaaaaac taaaagccgg catgatcgga
     9181 aaagcaaaat actgtaaaac taatgcgatc gcagcaaaca tcgctactgc aaccattttc
     9241 tgtacacggg tgttcttcat ttgaaatcct cctgttagga ctcctcttca gcgaactttt
     9301 tgacgtttct ctccaaatga aaaaactcta ttttccaggg aaaatagagt tagagttatt
     9361 tgatcaagcg tcgtcaaata agctctatct tctttatcca gactatactg tcggtatcgg
     9421 aatttcaccg attcagctat ttttttgcaa aataggtcgt ggactataac caccggtcgg
     9481 gaatttcacc ctgcccctga agacgaacct tatataattt ttgtttacat attcattata
     9541 catatctatt caaaaaacgc aacatctaac acttataaaa agtaagtgtt cattttgagt
     9601 ggttcagcaa actgctgact tcttttttgt tcagttcacg gtaatcccca ggacgtaggc
     9661 cttgtaaagt cagatctccg tatttttcac gttttagttt ttgcacagga agaccaactg
     9721 cttgaagcat atttttcact tgatgattac gtccttcgtg gatcgtcaat tccacgatac
     9781 tgtggtttgt cttaggatcc actgataaaa tttggaattt agctggagat gtttttcgtc
     9841 cttcgatctt tattcctttt gtcaaaggtg caagcatcga ttttgtagcg atccctttta
     9901 cctttgcaac gtaaacttta tccacttcat gttttggatg tgtcaggcgc tgagcaaaat
     9961 ctccatcatt tgtcagtagc aagatccccg aagtatcgta atccagtcga cctacaggat
    10021 agatacgttc ttttaccatc ggcaaaagat ccgtaacaac ttttcttcct ttgtcatctg
    10081 caacagcaga aataactcct ttaggtttgt acaataaata gtaaccgtat tcttcttgat
    10141 aaattgggac ttcatccact tccacagtgt catgtttccc aacttttatc cctaattccc
    10201 ttacaacttg tccgtttact tttacacgtc ctgcaaggat gaattcttct gcttttcgac
    10261 gagaagtgat gccagcgtgg gcaattactt tctgcaatct ctccattaca attattctcc
    10321 tttattcatt ccatcaaaga atagatctgg tgacacgtct tcttcaagtg aaatttccat
    10381 ttcttgtgta tcaggcagtt cttctaatga tttcaaacca aaataatcta agaagtagcc
    10441 agttgttcca tataatatcg gccgacctgg tccttctact ctaccttttt cttcaatcag
    10501 tttatgggca gctagacgct gtaccgctcc tgaaacctga actcctcgaa tctgctcgat
    10561 ttcagctctt gtgattggct gtttataagc gatgattgct aaggtttcga cggcggcttg
    10621 agataatgtg tttgatgtcc cttgcgcata tcgcttaagt aagggagcca gctctttttt
    10681 agtcgttaat acataatggc tttccgtttc aaaaatatgc aaagctgagt gcgagttttg
    10741 agtgtaagat tcttttaatt ctttcactaa acttgtgact tctgcttcgc tgagatttaa
    10801 cagtccgctt aattcttcat tactgatccc ttcctcgcca acaataaaaa gaagggcttc
    10861 aagttcactt aatttcgtca tgttcggtat ctcctactgc tggataaagc ataattgatt
    10921 catagttttc tttttgttgc gcaatgatct gccctttttt catcaattct agcaaagcca
    10981 taaaagtagt gacaacttca cttttagaag gtgactcaaa aaaagaatcg aaagtacagc
    11041 catgcgcatt tctttttata cgctcttcga taaataccat tcgttcttcg atcgttgtgt
    11101 catcgggcgt gatcgttttt tccacttttt gagaaaattt cttacgttct aagattttct
    11161 gcatggcacg gatcaaatcg atttttttga cttgtcctcg cttcaacggc ttttcagcat
    11221 ccgctaaccc atcgatgttc ataggttctt ttgtgtaata aagacttcgt tcctctgctt
    11281 tttctgtcag tctttcagct gcatatttat atttacggta ttctaaaagc tgagtgacca
    11341 attcttctcg aggatcttct tcccataatt cttcatcgtc tgtgacgaat tcttggacag
    11401 gaagcaatgt cttgcttttg attgccatca atgtagcagc catcactaag tattctcctg
    11461 caacagctag ctcgagtgtt ttcattgtat ggatataatt catatattgc tccgtgatcg
    11521 tagcgatcgg tatatcatat atatcgattt ccatttgctg aatcagatga agcagcagat
    11581 cgagaggacc ctcgaataca tctaatttaa tattgatctc cgtcaactca ttcactcttt
    11641 cttctttttc tttgctagcc attttccaat gtaaggctct gtgatttcag ttgcacggat
    11701 ggagacatcc gggtactttt cctgtaattc atctaacatt tgaaagccca tattctcacc
    11761 gcgataagac ggattcaaag aaatgtcatg gatgatcagc tgatcgttcg ttgcccagta
    11821 aataccaata atcccttgaa tgttggttga accttctggc gtaaagaaaa aaagttcata
    11881 atcgtcttca gattcgtacg tatcaatctc cttcaacaaa gaagttttac ctgtcagttt
    11941 cttatggaaa ctcaatagtc ccatagcaat tttacggttt tctttccggt aatgtgttaa
    12001 catcttctca ccttcctatg actaagccct tggaaaatgc tgcttataaa catccgtcat
    12061 tcttttcttc gtgatatgtg tataaatctg agtcgtagag atatccgcgt gacctaacaa
    12121 ttcctgtact gtccgcaaat cagctccatt ttctagcaga tgtgtggcaa agctatgccg
    12181 cagcgtatgt ggtgttactt ctttgtaaat cccagcctct cgtactaact gcttcagatt
    12241 tttccagatt ccttgccgag aaagaccttt cccatgatta ttgacaaata catgcatttc
    12301 agatgcgtcc tttacaagta atggacgtgc ttcgtctaag tagcgttcca gccattggat
    12361 cgcataatca cctaaaggaa tgatccgctc tttatctcct tttccaactg tctgtaccag
    12421 tccaagagaa agatgaagat ctcccaattt caatccaatc aattcactga cgcgcatccc
    12481 agtagcatac atgacttcca agattgcacg atctcttatc cctaacgttt ttgtcgtatc
    12541 aggtgcttca atcagctttt ctacttctgt cagagtcaat gtacttggta atttctgtgc
    12601 tttcttcggc gtatcgatat gttgcatcgg atcatgatca gtgatacgct cttgccgtaa
    12661 aaactgatgg aatcttctga gacttgagat catccgagta acagttgctg tcgctttttt
    12721 ttcttcatgc aacttctcca gaaaacggat gactaaaaag cgatcgacat cctgccatga
    12781 agcaattttc tgctcctgta gaaaatgcaa gtactgttcc aaatcacgtt catagctttg
    12841 tcgtgtattg gcggacaatc cccgttcaat attcaagtag tggagataat cgttgatttg
    12901 ctctttcata ataatccccc tcatgcctct ctatcatatc agataccgct tcattctgat
    12961 agtctttttc cataatctga gcaaagaaaa agccgtctag acactcagac ggactggttt
    13021 cttttttctt tttcatagca ttcctgacag attccatgga acgttagccg atgatctttt
    13081 actaaaaaat gataacgttc ttctactact tgctccactt caagtaaaag atcctcttct
    13141 acttcctcga tcgtgccgca gttcaagcat agtaaatgat gatggaaatg tttggcgcct
    13201 tcttttctca aatcataacg ggctaatcca tcattaaaac ttactttatc gacaattttc
    13261 aattctgtca agatttcaag ggttcgataa acagtagcaa gtccaatttc cggactcttt
    13321 tttttcacta aaaagaagat ttcttctgca gataaatgct ctttctcatt ttccaaaagc
    13381 acaagcagag tagcttctct ttgcggtgtc agcttaaacc ccgcttcatg tagctgctgt
    13441 tttgtttttt tcatcgccga aatagcattc atgaccattt actcaacccc aacaatttat
    13501 aataattaca aataaaaaaa gatataacac tatgttaatt aaaattatta taagctagtt
    13561 aagaaaaaca tgcaagggat tctcaattag attttttgac tagttttgtc acaccgtttt
    13621 cttgtgtaat caatccttgt ttcaaaagat tacctaatgc gcgcttaaat gctcctttgc
    13681 tgataccaaa agctttcatg atttcttctg gatccgattt atctgtaaag ttgatttgct
    13741 gatctgcaga acgttctaaa atggttaaaa tcatttgtgc atcatctgat atagcttcat
    13801 atgctcttgg cttcaatgaa agattcagta caccgtctgg tcggacacca atcactcgac
    13861 cttttatctg ctcgcctaga cgaggttcat tatatcgttc agaaggatgg ataaacccaa
    13921 gatagaaatc ttccgttaaa acgtacgtac ctgttaattt caagcggtat gctgttccta
    13981 tcacatcttt gtttttcatt tcttctgttc catatttgct taaagatttg aatatttttt
    14041 catctgctaa actgccccaa atacggtctt ttgcatcaac tctaagggaa atcatcaagc
    14101 gatccccttt tttaggccat agctctgtca ttgtcggcaa ttcatcaaga gatacgacaa
    14161 agtctttatc tggaagaccg atatcgacaa acgcacctaa atcttttcgt gtagcagtaa
    14221 cgattccaaa agcgtaatga ccgatacggc ttttaggaat ttgtgtagtg atccgatttt
    14281 catgtttttg attttggtaa ccgaatcctt caactgcttc accgattttg tgttcacctt
    14341 ctgatttgct taagccaaag gtttgtccgt ttttttggac aaagtagttt ttttcatttt
    14401 catcaataat aatacctgta aatatagttg ctaataaatc gttcattttt ttctccctct
    14461 atagtgaaag gttttcttaa tacatttctt tgctatcata acataatgtt atctacttga
    14521 atagctgagc cttggtttgg aaaggtgaaa aaaggagtat gacaacagtt gtgtcatact
    14581 cccctttttc gaataaacgg tgttaaacgg caggctcaca gcctctttca tttgcctaaa
    14641 attaattgct cccatttcct ccgaaccatc cagttttaca gttgatggtt acgatgtaaa
    14701 atggcaaagc actttctcca atgcctttca tcaatgcaga ttttgttgaa gtggcatata
    14761 gattataata gccttcccca ttttcgattc tggctttttc taatctgtaa cctccttcaa
    14821 cgatatatcc acgttggata catgtagcaa tcactttgct ttggacacct ggtgcccacg
    14881 tccatgctgg attagatgca tcggcaatat cacttgctct ggcagatttg attggctgat
    14941 tcgctgagtt tgaagaggaa ggggtcgagg atgccgcttc ttgctgtgct gcttgtgcga
    15001 ccgcagcttg ttgagcggct tgagctgcag ctgctgcttg agcggcttgt tttgcttgtt
    15061 cttcagctgc ttttttcgct tcttctgctt ttttcttttc ctcttcttct tttttctctt
    15121 tatcagtgag catcttatct acttgtttca attgttcatt caactgagca gatagatctg
    15181 tatttttcac tttttctact gcttcttttg ccgtcttgta ttggtcgcgt gttgctgaat
    15241 ccactacttt tccgtcttta aagactatag ctgttttttc tttagctgtt tgcaattcag
    15301 catattgatt tttagcttct gtttgtgctt gttgaatcaa ttttccaaaa tctgtgttat
    15361 ccgtttcgat tgcttggatc tctttatctg ctttcaactt gatgtcatct gctagatgat
    15421 catcgacgat ggcaggtgct atgaaccact cattcacttt ttggatatct cggatcttag
    15481 attggatatc ttggtaagtt gtttctaact ctgaatatcc tttttcattc ttaagactgg
    15541 ataaatcatt ttttagtttt gataaatctt gatgaatcat atctgcacga ataaattgat
    15601 ggtgatcatc tgtataaaag gcagcaagtt tctcatgaag ttcttccatt tgttgctgtc
    15661 tttttgcttc tgctgctact tcggcttcta ctttttgatg atggtcataa taagcccagc
    15721 ttcctccacc gagtagaagt actgctgcta aaacgagata aggtactttt tttgattttt
    15781 tcttttgcgc tttatctgga tctgtttttt ctaagtttga ttcttgatgt gttttttcag
    15841 tatctggcac gattgttatt gatttcgctt ctgcttcaaa cagcttaggc tggttctctt
    15901 ctttttctat ttttgcttca agatcagtac cctctttgct tacagattga ttttcagcat
    15961 cggattcact atttatttca tcgggctctg ttgacttttt gtctgtgttt tcgagtattt
    16021 cttttttctc tgcttccggc agtttattcg ttttttcctt ttctaattgt ttttcagtaa
    16081 atacttcagt tttttctgtg atatccgttt ttacgtcttt tatttttact gtttctttac
    16141 ctgtggcgtt ttctctatca gagtcttttt cagtttcttg ttccttttct ggctctactt
    16201 tgttttcctg tgcaattttt tcactgatca ttttgagcag ctctttcgcg tgctcttctt
    16261 ttgtatcttc acgatgtgct ttgatataag cggatagtat cgaattttct tcctcattat
    16321 cgacgatttc tttcagttca gctactcttc ttttttgttt tgattcaagc gtttcttctt
    16381 gattctttcc tgattcttcc gaagtctcat gaacagttgc tttcttctgt tcatttttct
    16441 ctatattagt tacttgatcg tgagcaggtg gttcttcgtc ttctggttct gctttttcga
    16501 ataattccat tacagattct agaggtaaat ctttcaattc cgaccattcg attggatcgt
    16561 ttaattctgt atcaggaaag actgcatctt cagttgattt atcctgcata gctgcatctg
    16621 tatttgtttc ttcaattgat tcttttattt gctgatcttc tgcagttatt tgatgaccgc
    16681 agtctggaca gatagtattc tgctccattt ttttcgatcc gcaatttgga cattttttca
    16741 tcacttagcg atcggctcct ttactaggtt tacgctacca tttaatctat catattctat
    16801 acgtcacgcc aatagcttat ttttaggatt tacttaaaaa taatgaatga atttccttcc
    16861 tatagaaaaa aaagcttgag actgcgtagt ctcaagcttt ttttctttta aaataaacca
    16921 aacagtttta ctgatttctc ttctggtcaa tagtccacgc ttattctaag aaatctttta
    16981 attgttttga acgagaagga tggcgcaatt ttcttaatgc tttagcctcg atctgacgaa
    17041 tacgttcccg agtaacacca aatactttcc ctacttcttc caatgttctc gtccggccat
    17101 catctaaacc aaaacgtaag cgaaggacat tctcttcccg atcagtcaat gtatctagta
    17161 catcttccaa ttgttctttt agtaactcat aagctgcgtg ttctgctgga cttgttgctt
    17221 cctggtcttc tatgaaatca cctaagtgag agtcatcttc ttcaccgatt ggcgtttcaa
    17281 gggaaacagg ttcttgcgcg attttcaaga tttctcggac tttttctgtt ggaagatcca
    17341 tttccgcacc gatttcttct ggcgtaggtt ctcttccaag gtcttgcagc aattgacgtt
    17401 ggatacgtat caatttatta attgtttcaa ccatatgaac tggaatacgg atcgtacgtg
    17461 cttggtcagc gattgctcga gtgatggctt gacgaatcca ccatgtagca taggttgaaa
    17521 acttaaatcc tttgcggtag tcaaattttt caactgcctt catcagtccc atgtttcctt
    17581 cttggatcaa gtcaaggaac tgcatgcctc gtcctacata acgtttagcg atacttacta
    17641 ctaaacgaag gtttgcttct gccagacgct gtttcgcttc ttggtcgcct tcttcgattt
    17701 tcaatgctaa cgctacttct tcttccgcag ttaaaaggga gacacggcca atttctttca
    17761 gatacatacg aaccgggtca ttgattttta caccggttgg cgcagaaaga tcttctgttt
    17821 ttgcttgttc agcttttttc tcagcactct tcaagctgtg aattgatgga tcaccatttt
    17881 catccaccac actgatacct gcgtcttcaa tcttttggat caatttgtcc atttcatctg
    17941 cattcaaagt gaatggtgta gcaagctgat tggataaatc atcatataca acttgtcgtt
    18001 tgggtttgtt ttctttgatg aaagctgcta ctgctgcttc atacgttttt ttatctgttc
    18061 catttgccat aaaagaaggc cccccttgag tagctatttg gcttgtttta actgtttcgt
    18121 taaattgatg atttcaatcg tcaattcaag ctcaagctgt ttgtttccag ttcgtctggc
    18181 ttcttgctgc atttgttttt ttaatgttat ttcatcagct aagccagatt tgctgatcac
    18241 atctagcaag tcttggattt ctcgtggcga actttcttct gccaccggta aatattcgat
    18301 ttccaccacc aaccgcctta aattttcatc ttgcaaaaag tctaaaaact ctgcaaggtt
    18361 gaattcataa tgttgtgaga catatgcatc gaataaaaaa taaacttctg catatgcatc
    18421 atgaataaac tgaaattctc gttcttttaa aatattacgg acagaaactt ctttaaatgc
    18481 acgatataat agaatctgct ctgccttttc tacttgcgtc aaaggtctct ttttccgtgc
    18541 agtttcttgt ctcgtaacct gagtgtgttg actttcctga ggttcttgac gattttgctg
    18601 atttgtcctt cgaagatcgc gcagttgttt ttgtatcgtc tctctgccga tctgaaactc
    18661 tgttgacagt tgagatagat acatatcttg ttcgattggt gaaacaactc ggctaagttc
    18721 aactagtaat tcttcgaggt attcgatctt gtctttttca ttatctaaat ttttcccttg
    18781 ttttaaataa cgtgatttaa aagtaaagac tgtttcgcgc ccatgagcca ctaattcttg
    18841 gaaagatgct tctccatact tacggagata ttcatcagga tcaagctttt ctggaacgac
    18901 tacgacagaa agtgctaatc gactgttttg ccgaagcaat tcaatccctc gattggttgc
    18961 ttcaaaacct gcgttgtctc cgtcatagca aaagaccaat tctttcgtca gcttttccat
    19021 ttgttggatc tgttgagctg ttagacttgt ccccatagaa gctaatccag ttttgatacc
    19081 agcctgccat gcagcaatca catccataaa cccttcgaac aacaaaattt ctccgttttt
    19141 ccggatatcg cttcttgctt tatcaagatt aaataaaact tgtcttttat tgaacagttc
    19201 tgtttctgga ctattcaagt atttgggctg atctttttcc tgcccttttt caggtgcaag
    19261 ccagcgtcct gaaaaaccga tcgtctggcc ttgagaatta cgaagtggaa acatcactct
    19321 ttgataaaaa cgatcagaca tacggccatc atcatgcgtg acaaacaatc cagagcgttc
    19381 taacaattca tttgaaattg attcattctt gaatacttgc tgaagaaaag aacgctcatt
    19441 aggtgcaaaa cctatgttga attcttcaat taattctaaa gacaacccac gactttgcaa
    19501 atattctaaa gctggctgtc cagcttttgt atgcaatagc atgtgatgat agatttccgc
    19561 tgctttttca tgagcagcaa tcagtttccc cgtagcagat tcaggatttt ggacagctat
    19621 agtacgctga cgccattgat cttctaacgg tatctgttcg aagtctgcca cttttaccac
    19681 tgcttctgga aaacttagac cttctagttc ttgaagaaaa gtaaacacgt ttccgccttt
    19741 accacaacca aagcaatgaa aaatctgctt gtcttctgca acagaaaaag acggtgtacg
    19801 ttcttcatga aaaggacaca gccccaagta atttttacca gactttttca actggacata
    19861 ttgtccaatc acctcaacga tattcgtttg actgcgtact gtttcgataa cctcttgagg
    19921 aatccgttga gccatttttt caccccctgc tctattgata tcttttgggt ttaacagaag
    19981 atatttcagg agaataaccg gtaaaaaatc gcactaactg aaatcagtgc gattttaaag
    20041 gacacaatta tacatgtttg attgtatcat atttcattgc tttttcaagt ataacacatt
    20101 tctatctcat taagcatcaa tttgctactc ttttcagtaa ctgattttga atatcagcct
    20161 tccagtacaa tactcgaaga acaatattta cgagcatgaa tacaaagaag tatcctacgc
    20221 tctctcccaa ccctaagttt acttccaaaa ttccatggaa cagcattgca gctacatata
    20281 tccctacgat cgtcgttact gctaggaaga tgcgcaatgg gttgaaggga taacaagctt
    20341 ttacaactgc catgcaactg atcccaatca gcaagtaata catcaaagta gtagtttcag
    20401 catttcccca tgcgaatgtt tttccaagta gatacaccgc aatgatattc actactacaa
    20461 gcaaggcatt tggtaaagca ttaataagtg ctgtaggcaa ataacggtaa gtgatttttc
    20521 ttttatcttg ttcaaaagat aaaaagaagg aaggataccc ttcaatcgct aaatcaatca
    20581 gcgtgatttg gatagggata aacggaaatc caattgctgt aacggcacat ataatcgata
    20641 aaataaatga ataaatcgtt ttgataaaaa agatgcctga gactttcgtc acattgttga
    20701 ccactcggcg tccttcaaac aggacttctg gcaatgtagt aaaatcagag tccaacaata
    20761 ccagattcgc aatctgacgt gttgctccat caccttctgc catagcaatg ctgcaatctg
    20821 cttctcttaa cgccaaaaca tcattcacac cgtcgcctgt cattgcaacc gttctgccgc
    20881 tttctttcaa ttcatttacc agcaatttct tttgctgcgg actcactcgt ccaaaaaccg
    20941 tatagcgatg agcagcttct cgcacttcca tttcttcagt aatttgagaa agatcgacat
    21001 acgcatcata attcggtaat cctgcacgtc tagcgatatt ggagacagta aaaggattgt
    21061 ctccagaaat cactttcaga tccacacctt cctcatgaag ataagctagt gtttcttgag
    21121 cgttttttcg gattggatca tcgatttcaa acagagcaac cggctcaaca aatgctggtt
    21181 tttcttcgtt caattgctga tttggagcga tccccaacat aagtacgcga tagccattcg
    21241 cttgggctgt ttttagactg tccggaagcc gatcttcttc caccaaacgt tcaggcgctc
    21301 ctacgtacac gactcccagt tctggaaatt ccatcgcgcc ccatttgcga tcagaagaaa
    21361 atgcgacttt gtttgtcaca tcataccgat tagaaagctc aaaataatct ctcatagccc
    21421 gcatagtgat gttgttatca ctgctttctg ccagatacgt ccccataatt tctgggatgg
    21481 ctttctcgta cagcggatgt agtgcttcta cccgctggat actcattttc ccttcagtaa
    21541 tggtccctgt tttatccaag cataatgtat cgacatgggc cagcgtttca attgaataca
    21601 tgtcttgaac aaggattcgt ttttttgcca gtttggttac ccctgtcgtt aatgcaatac
    21661 ttattagcaa taccaaaccc tttggaagca tgcctaacaa agctgctgca gatgcaataa
    21721 cagaaatttt gatagaagca tcgcggatga acagtgcttc caaaaatagt atgatcccca
    21781 aaggaataat cacgaaacta gtgaatttgg atactttgcg aatcgaatct acaagttcag
    21841 aacgaatagc tttgtgagtc tttgcctcgc tagtcaagcg gacagcataa ttatctgcac
    21901 ctactcggat aacttcagcc atgacagaac cgcttgtcaa aaagcttcca gaaagcaatt
    21961 cagctccagt ttctttgaca atcaaatcag attcgcctgt caaaagagct tcatttgctt
    22021 ctgcccgtcc agatataacc tgtaaatcgg ctggtatctg ttctcctgct ccgattttta
    22081 ccaaatctcc taataccagc tctttagtag aaatcgattg ttctttaccg tcccggatca
    22141 ctagtacttc tgattgggaa acaatcgata atttccgtac aagatttctt gcatgtattt
    22201 cttgataaat tccaataacg atattgacga caataattgc taaatacgcc atgttggaat
    22261 acgcacctac aaataacaga caaatcccaa taccaaagtt cagaaaattg aataacgtca
    22321 atatattatc gctgaatatt tgttttgtac tttttgatgc atcttcctgg tagtcatttt
    22381 gctggccaga agccattttc tgaactacct cttgctgtgt aagtccattc attttctttt
    22441 tctccatttt ccgccaactt tcaaaaataa tcacgtgttt aagtctagcg tgttccaaga
    22501 ttaaaaacaa cttgttttta taaaatttat agaaaaacac acgaatgatt ttacaaaaaa
    22561 gatgccaacc tttttgtttg aatcacccgt gtgctgtttt tattatcaat ctatctttac
    22621 ttatattgtt cctccaaacg gctcatccac caacctagtg ctgtaccggc tataaaaaga
    22681 acaggacaag ctagtacccc agcaactccc aatcccgcgt aatcggtagg aatgatcata
    22741 atcgttgaag caaaaactac acctaaaatg aaatggaata gcttgctata tgcctttgag
    22801 aaaatgtaat ccatgatttt tgacaatcca agaacacaaa gaatcccacc aattccgatc
    22861 ggcacaatca cttcaagtcg tacttcttta aatccgtcag ccatcgcttt atacaatccc
    22921 atatacacca aaaaattaga agggctcaat cctgggacga tcatacccaa accaatcaat
    22981 ccgccggcaa tcagccatgt tccaaaattc tgatcgactt gcccgtcaaa taattgagcg
    23041 ccgtataata aaaacaaata agccagaaca aaagtaacaa ccatgatgat cacatcagaa
    23101 gtatctcttc cttttttccc tgcttctttc cataacgcag gaacagttcc agcgatacat
    23161 ccgacaaaaa accataatat cgttgtttca tacgcaccta ataaaaaact gactaaaaaa
    23221 gaaagtagaa agatgcctgt cactccgcct agaccaacag gcagaaaaaa caaaacattt
    23281 tctttgaaat tttttgtaag gtgggctaaa aaagaaatca gccgctcata catgccaaag
    23341 acagctgcta atgcaccacc gctgactcct ggcaagataa atcctgaacc gataaacatt
    23401 cccttcacaa accgtaacat ccagtctttg gttgtgtacc ccacttcttg attttccatt
    23461 ttttgccttt tttccactat ttgcgtactc cttctttcat ttaattttat tcatgattga
    23521 acatttcctg ctattttact taaagacttt ttcataggtc tctattattt tttcattcat
    23581 tttgacaata tcaggataaa acatgttacc tccattatga tagagatagc cgccattgta
    23641 aagcaaggac tggatccgcc aatatcggta ttgtttccct tcctcatttc ctaaaagtgg
    23701 gctcagcaca tctttagaat acgcttctgc taattctact gtgttttctc cgccatgatt
    23761 ttttacatag gcaatataat ctttcccaaa attatatgct tggacagctg tccataaatc
    23821 gcatccttgt ttctttgctt ctactatcat ttccgccaaa tatttgacac cttgatcaat
    23881 actgtcagct ggattatcaa tgatctcagc ttctccatgc agactttccg agctctgcat
    23941 gagatctgtc ccttttcctt ttgattcggt caatattatc gccttgatca aattctcgta
    24001 gttttccata tgataatttt ttgcagctgt tgttatttct atttgatagc tttccactcg
    24061 ttttaccgaa cgataagttc ttcctcataa aaacaacacc gcaccaataa ccgtcagaat
    24121 gatcatgtaa aaaagaatac ggataataga taatcgtttt cgcttcattt catcacctag
    24181 ttattttgac atcttttttt atcttactat ttctttatct tcttggctat ttcttccttg
    24241 atttctgtgt acgccttaag aaaaaatgaa ttttcctgta ttttttgaca tcttacgaaa
    24301 atgatcgttg tttttcagta aaaacaattg cccaatgact gatataatcg ttatacttta
    24361 cacagtaggt gattatgtga aaaaagaaaa gaaatatgca aaaatgccag atgggagcga
    24421 aatttactat gaaaagagcg gtcaaggctt ccctctcttt ttactgcatg gcaatgacgg
    24481 cagcggccgc tttttttcag aacaggtccc tgtactcgaa cgctactata ccgtgtatct
    24541 agtagatagc cgaggacatg gtcgttcgac aaacgaagca tctatgttga attttcagtt
    24601 aatggccgag gatctgaata cgatcatgtt gctggaaaaa atcgatcagg cagattttct
    24661 tggatttagt gatggagcaa atctagcact ggtatttgcc agcagcttcc ctaaaaaagt
    24721 tcatcgattg attttaaatt ctggaaacac gcttgttaaa ggcgttcgtt tttctgccag
    24781 agtcatcagt aatattcatt atgcatgggt atggttactc tctttatttc gcccaagtct
    24841 tcgcaaaaat cttttagtta tcaaactttt gctgcatgac atcgggctta cagaaaatga
    24901 tttaaaaaaa atcaactccc ccactttgat catcgtggga aaaaaagatg taatcaagct
    24961 aaaacattct ttgtatatag ctaaaactat accaaaagct tcctttgtgc ttgtgaaaga
    25021 acaaggacat gaattagcta gaaaagatcc tgaacgcttc aatcgagaag ttcttcaatt
    25081 tctttctgaa acataactat taggaataga aggtgattta tttgttaaaa aataccatac
    25141 aatggctgaa aaaccaccta ggactgttca aaacaatctt tttgatttct gtcattgtca
    25201 ttattgtcgg tgaattgatg tccatcggta aaactctatc tatccagcaa ttaggagaaa
    25261 cgtttgccac tatacctgta tggaaaagcg gactaatgct attgattggt cttatctctg
    25321 tgttacctat ggttggttat gatattattt tgaatcggat gcttgaacaa aagcagaatc
    25381 ctcgttacct gtttgagaca agctggctga tcaatacgat caacaatatt gccggtttcg
    25441 gcgggtttgt tagtgtgggg cttcgctctg aactatatgg acaaaagaaa gagagcagaa
    25501 acgtgatcca agcattatcg aaagttttct tgtttttgat ggctggactt tctatttaca
    25561 gtctgctttc ttttttactt gtagtcttca cgccagttgc tccttttttg aagcaatatt
    25621 ggatatggtt gatcggcggc ggtctatatt ttcctgtcgt ctttttattt actactttca
    25681 aaaagaaagg attccttggt ggtacctcac ttaaaactca aggacagcta ttgctagttt
    25741 cattgttgga atggaccggt gtcctgttta gtttcctttc tgttggttat ttaatggaaa
    25801 tccggattga tctgtggcaa acaattccat tattcattgc tgcttcagtg ataggaatcg
    25861 tgtctatgat tccaggagaa attgggagtt tcgatgtaat gatgattatc gggttgtccg
    25921 ctattggtgt gccaagagaa atagtcgttg tgtggatctt gctctatcgc ctgttctact
    25981 acatcattcc gtttttgatt ggaatcgtct ttttcttcaa aaatatcggt tccacttttg
    26041 atcagcgtta ttctggtatc cctaaacaat tagcaacaga aattgcgcat aaaatcgtgg
    26101 ttgtcttatt atacttttct ggaatcatgc tagtactttc tgccactatc ccgcaagctt
    26161 ttacagagtt tcgctggctt catagtttga acccattgaa atttcatttt attatccaat
    26221 ttcctagtat actattggga tttcttttaa tcattatggg cagagggatc gcagcacgtg
    26281 tgaaacgagc atatttgcct acaatcttct tgattgttct agctttactt tatgttttat
    26341 taagtgattt tagtttcaca ccggtggttt tcttgtctat tctgcttctg atcattcttg
    26401 cttctaaaaa cgaattattt cgggaacaat tgatctattc atgggagtgg cgtacgatcg
    26461 atggaatcat tattggtgca ctgtccttgc tttacatcgt tattggggta tacaatctgc
    26521 cggatttccc tcatcggcgt caccatttta tttccttttt cttatttcct tctgaaaaga
    26581 tttggttttc gggattgttg gccatcatcg ctgttagttt catgatcgtt ctatttgttc
    26641 atttccttca aggagaaaag aaacagattg gcgaagcctt taatgaagaa aaggccctga
    26701 agatccttac aacttatgga ggaaactccg atagtcaatt gatcttctta aaagataagc
    26761 ggatgtttgc ctatgaaaaa gacggtgaac caactgttct tctccaattc gcttgcttca
    26821 ataataaatg tattgtaatg ggtgatcctt ctggtaagaa agaagatttt ccagaagcaa
    26881 tcgaagcttt tatcgaagaa acagatcgac tatgctattt accagttttc tacgaaacaa
    26941 gcgaagagat tgttatgatt ttgcatgaat tcggctacga tttcattaag atgggggaag
    27001 aagcttatgt agacctgaat agttttacaa cttcagggaa aaaaatgaag ggcactcgtg
    27061 ctgtcttgaa tcggatcgaa cgagaaggat ttacatttga tgtcttacaa ccaccatttt
    27121 ctactgagca aatgagtact ttcaaaaata tctctgataa ttggttgggt tcacgaaagg
    27181 aaaaagggtt ttctttaggc tttttctctg aagattatct acaaagagcg cctattgcag
    27241 ttgtcaaaaa tacccaagaa gaaattgtcg cttttgcaac gatcatgcct acttacacga
    27301 acaataaagt aggaacgatt gatctgatgc gatatgattc taaaaaagca ccttctggca
    27361 gtatggattt cttgttcttg catttgtttg cctatatgaa agaagaacat atccaatggt
    27421 tcaatttagg gatggcccct ctttcaaatg tcggaacttc cagaaagagt tttttacagg
    27481 aaaggattgc atacttggtt tacgagtttg gttctcattt ttattctttc catggattaa
    27541 gagaatataa aagcaagtac gctacaaact gggtctcacg ttatactcta tattctagag
    27601 atagttggat tgcatatgtt atgattgctt tgctgattat tgataatgcg ccagttgagc
    27661 agacacaagg tagaataagt ggtttgaaaa aatggatacg aagaaagtat taaaaactgc
    27721 ataccgatag atgaacactc tcatgatgaa taaaagagac tgaagtaaaa cgaaagttca
    27781 tgcttctaca atctcttctt atacaattga agaacaaaga gcggtccaaa cagaaatcct
    27841 gtttggaccg ctcttttatt ttaaacattt ttcattacca aaatattcga gtcgcgtttc
    27901 ttatgcttcg atctctgtaa cgatacctga acctactgtc cgtccgcctt cacgaattga
    27961 gaatgtcgtt ccgttttcaa cagcgattgg atggatcaag tcaacatcaa tcgtcacgtt
    28021 gtcaccaggc atgaccattt ctgtatcttc tggcaatgtg attgttcctg taacatcagt
    28081 tgtgcggaag tagaactgtg gacgatagtt gttgaagaat ggagtatgac gtccaccttc
    28141 ttcttttgtc aacacgtaca cttctgcttt gaattttgta tgtggagtaa tggaacctgg
    28201 tttagcaagt acttgtccac gttcgatatc gtcacgttgg atcccccgta acaacacgcc
    28261 tacgttatcc ccagcttccc cgtaatctaa cgttttacgg aacatttcta cacctgttac
    28321 aactgctttt tgtgtttcag gtttgatccc tacgatctct acctcatcac cgacacgaac
    28381 agcaccacga tcaatacgac ctgaggcaac agttcctcga cctgtaattg agaagacgtc
    28441 ttccactggc aatagtaatg gtttatctgt atcacgttct ggtgttggga tatattcatc
    28501 tactgtatcc atcaattcca taatagcagc ttcagcatct ggatcgcctt gcaatgcttt
    28561 taatgcggaa cctttgatca caggagtgtc atcgcctggg aaaccatatt cgctcaataa
    28621 ctcgcgaact tccatttcta ccaaatcgat caattcttca tcatcgacta aatcaacttt
    28681 gttcaagaaa acaatcaggt attttacacc aacttggcgt gacaataaaa tgtgttcacg
    28741 tgtttgaggc atcggaccgt cagttgcaga aacaactaag attgcaccat ccatttgtgc
    28801 tgcccctgta atcatgtttt tcacatagtc cgcgtgtcct ggtgcatcaa tatgcgcgta
    28861 gtggcgtttt tctgtttcat attctacgtg tgcggtattg atcgtaatcc cacgttcacg
    28921 ttcttctgga gctgcatcaa tactagcata gtcttgagga tttgctaggc cttttttgcc
    28981 taatactgta gtgatcgcag cagttaaagt agttttccca tggtcaacgt gtccaatcgt
    29041 accaatgtta acgtgtggtt tacttctgtc ataatgttct tttgccataa tttacaaacc
    29101 tcctaaataa tatagatcac tgaaaaattt cagctctcac ctttttatta tagtcttttt
    29161 ttgactaaat caaaaagaat acgtccgact tactttttgt aagattcgaa ctaagcttat
    29221 tatacgtcaa tattggtcaa agtcaactaa gagcactcgt ctgtcttata cgcgaaaaaa
    29281 gtacctttta taaataaaaa aatgtgtttt ttgagaaaaa cttcaagata gtccttaact
    29341 ttcttttgaa gcaataagaa caaaatgagg aaaagtttaa aattcagctg ttgccaacaa
    29401 ctttcaatag acgaaatctt ctatttcgtt aataataagg aaagaggtga agtacatgaa
    29461 aaaattaatt ggcgtacttg tcgttttagg catatttctc ggcggaagtt ttgcagctta
    29521 tcattatttt tacggcggcg aagcttatta tacaaaaatc acgacgaatg gcgaaatgtc
    29581 agctacccaa gcagataacg gagagaaatt cacgacttat acctacaagc aagcggctta
    29641 taataaagat ggtgaaaaaa aacaggtgac gcttcgcgaa gaaagagaac atccattaaa
    29701 gatgaatgcg tatcttaaac tcaaagtcaa tcctcgtaaa ggtgtattaa gctgggaaga
    29761 aataaaggct tctgaagtgc ctaaaaatgc ggcagaaaaa atcgataatt agtaaatggt
    29821 cttaaaattt tattagataa aaagaggctg ggacagaagc gtttaactcc aagaaataag
    29881 aaggagttgc ttctgcttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
    29941 ttacttttgt ctcacgctct ttttttgtta tattcgtctt ttccgatcct tctcttgaca
    30001 caattcattt aactgatcac tggtacttga acacctaata ttctagtcat ttttataaac
    30061 gattcaatac ttcatccaca tttttgatca tcacggatat cgtaccatcc ggattattca
    30121 caaattcaat caaatctggg ttgcgatata catctactgg tacgatcagt tcgatcccgt
    30181 ttgatagctt gaatttttgt tttccaaatt ttttctcaga aatttcttgt acttctttta
    30241 caggaggtgc ttctgggaca aatccttgct cttgtacttc ttctttaaaa gccatttttg
    30301 ccgtgatgtt ttccttgaag actttttccg caatcatctc atgatccaac cgaccttttt
    30361 cttctatcgt atcatagaca gcttctttca aatcagccat gatatcaaat tcttctgtct
    30421 caaatttttt tccaagatgc ttggctactt tttggatcac ttttacattt tcttctaaag
    30481 aaggtgcagg aacactttcg atcactcttc cagaaaaata aagctctttt ttgccagaaa
    30541 actcatattt tttttcaatt aattcgtagg tccaatcagt caaattcaca gcgaatgctt
    30601 catctgcttt ttgtgttttc gcccctagaa ttgctcgatt caaaatcaag cggttgtcga
    30661 tccctgcttc gtctgcttca acaaagtgag tgtaagcctc acgataattg actttcaata
    30721 aagataagta catcactgta tctaattcat acaagacaac aaatacatct gcactaggtg
    30781 cttcttcact ttcttggtag atatcgaacc accgctgaac gattgcttca cttgcttcca
    30841 caaaattttc ttgagcgatt ttaagtgatt caacgatcat gctttcttct gctagttgtc
    30901 cagtttttgt ttgagcagaa gagatttttt ggatcttctt ttgaagaaaa tcacgaatat
    30961 attcttttgt cagatccagc tctttttgtg aaaagactgg agaaccagtt tctctgtcaa
    31021 taatatgcag aattgctttt tttaaataga tatccatttt ctcattccct tctgtcttag
    31081 tttacggaaa aaaaatcaaa aataccagaa tcaaaatcat tttcttttaa acaaaaattt
    31141 caagagtaga aacaaaatgg attctttttt tcgaaaaatg cgctacactt atagttgaac
    31201 gaataaaaaa ggagctgaaa gtatgaattc attaacaagc acttatcgtc tatcaaatgg
    31261 ttacgaaatc ccggtagtag gattcggcac atggcaaaca cctgatgggg atgtcgctgt
    31321 ttcctctgtt aaagaagcgc tggctgcagg ttatcgccat atcgatactg cccaaggata
    31381 caagaatgaa gaaagcgtcg gccaagcgat taaggaaagt ggaattccta gagaagagat
    31441 attcttgaca acaaaattat ggaatgcaaa ccacagctat gaacttgtga tgtcttcttt
    31501 tgaagaatca ttgaagaagc tccaaacaga ttatttagat ctgtttttga tccactggcc
    31561 aaaccctgtt gcttttcgtg ataattggga acaagcaaat gcagatacat ggcgagcatt
    31621 tgaagaactg tatgaagcag gtaaaatcaa agcaatcggt gtgagcaatt tcttacccca
    31681 ccatctagat acactagcaa aaacagcaaa aattatgccg atggtcaatc aagttttcct
    31741 tgcaccaggc gaattacaga caactgtagt tgagtatgca aaaaaacatg atatgatcct
    31801 cgaagcatat agtccattag gcactggtaa aatcttcgat gtgcctgaaa tgaaacaaat
    31861 cgctgaagca catgacaaga cgattgcaca agttgctttg cgttggtcgc tgcaacacga
    31921 attccttcct cttcctaagt cagtgacacc aagcagaatc aaagaaaata ctgaattatt
    31981 tgattttgaa ttaacagaag aagaaatgaa acaaatcgat cagttagatg gtgtagtcgg
    32041 caaagcaaaa gacccagata ctacccaatt ttaagtcgaa aggtgatttt atggatccaa
    32101 tcgaacgctt gaattctctc tcggaagaag ttacccagac ttttcattcc gattttgtct
    32161 ttttgatcga tgcagagaaa atccaacatt ttcctgcaag aaactggaca catgatcaaa
    32221 tcatcgaaga actgaaaaaa cggttcgatc attcgctaat ggtcaaaccc tggcacgaac
    32281 atgaagtcat ttattcacct gaactacctg tttttgcctt gataccgaaa aaatagaaag
    32341 aatggaacgg attatgtata tatacataat ccgttccatt ttttattttt ctttcaaaaa
    32401 acgggaaaat acgcaaattt ttctgttgta atttgcgtat acgcaagttt ttttgcatta
    32461 ctccttttca tttggctgtt tttttcttat aatcgaaagg gtatgagcga aggagagaac
    32521 atatgaagaa aaaacagaaa caaaaattat taaatcaatt ccgtccttca ttggatggtg
    32581 taagaggaca attattccgt tctctagaag aagagagctt gcgacgttat ggtttgccgt
    32641 tacaagtcat gcttgatccc aaaaaccgtc aaatcttaac gatcggatta gctgggaaaa
    32701 caaatgaaga tacacggatc aacgtgccaa tcgatgacaa cttcaatact gtattaaaga
    32761 gaattcgttc aggagaaaac gggattttcg aacgattccg tgacaatctt cttattgaaa
    32821 tcgtgtctta ctggaatgat caacgattga aaaacgatga accaactgcg caagctgttg
    32881 cgacaccagt tacagaagaa acagtcagtg tgccagaaga aaaaacggaa aaaccaaaag
    32941 cggacacaac tgaaaataca cgctcaactg atacgaatgc cgctttaagt tttgctgatt
    33001 tttcaaaagc cgttgctgag tatcctaaat tttacacaga acaaaatgaa catgaagtag
    33061 ttattaatga aaaaaatgct gatgaacctc gtaaattagc aactatttct atgactactg
    33121 aaaatgagtt cgttatcgaa aaagcactcg aacggaaata caaagtcaaa ttgactttga
    33181 ttcctttgat cgaacaattt gcagcgacag cgattactga tcgataaaaa ttctatttgt
    33241 cataaaaggc tgggatttaa aatcccagcc tttttagtca tataataatt tctacagcaa
    33301 gtaaaaagaa attccttcta acatcaggct caaatatcag cttgacctgt ccaacaaatc
    33361 acctatgatc ctacaatgtt aggcgacttc tcttctacct gaagatatct tggagttgcc
    33421 tgatttctta agatgcctaa ccttaactat aaaaaattct aataataaac atattttctg
    33481 atagagaaaa aa
//
LOCUS       DACCYH010000025        33264 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.025, whole genome shotgun sequence.
ACCESSION   DACCYH010000025 DACCYH010000000
VERSION     DACCYH010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.025"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(42..230)
                     /locus_tag="IVY85_001109"
     CDS             complement(42..230)
                     /locus_tag="IVY85_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP8044138.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            500..862
                     /locus_tag="IVY85_001110"
     CDS             500..862
                     /locus_tag="IVY85_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8044139.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            914..2596
                     /locus_tag="IVY85_001111"
     CDS             914..2596
                     /locus_tag="IVY85_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP8044140.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2713..4749
                     /gene="recG"
                     /locus_tag="IVY85_001112"
     CDS             2713..4749
                     /gene="recG"
                     /locus_tag="IVY85_001112"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP8044141.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4798..5799
                     /gene="plsX"
                     /locus_tag="IVY85_001113"
     CDS             4798..5799
                     /gene="plsX"
                     /locus_tag="IVY85_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP8044142.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5921..6163
                     /gene="acpP"
                     /locus_tag="IVY85_001114"
     CDS             5921..6163
                     /gene="acpP"
                     /locus_tag="IVY85_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8044143.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6431..7435
                     /locus_tag="IVY85_001115"
     CDS             6431..7435
                     /locus_tag="IVY85_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8044144.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7436..8380
                     /locus_tag="IVY85_001116"
     CDS             7436..8380
                     /locus_tag="IVY85_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8044145.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8380..9342
                     /locus_tag="IVY85_001117"
     CDS             8380..9342
                     /locus_tag="IVY85_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8044146.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9369..10283
                     /locus_tag="IVY85_001118"
     CDS             9369..10283
                     /locus_tag="IVY85_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8044147.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10309..12090
                     /locus_tag="IVY85_001119"
     CDS             10309..12090
                     /locus_tag="IVY85_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8044148.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12438..13124
                     /locus_tag="IVY85_001120"
     CDS             12438..13124
                     /locus_tag="IVY85_001120"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8044149.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13144..16725
                     /gene="smc"
                     /locus_tag="IVY85_001121"
     CDS             13144..16725
                     /gene="smc"
                     /locus_tag="IVY85_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP8044150.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16734..17543
                     /locus_tag="IVY85_001122"
     CDS             16734..17543
                     /locus_tag="IVY85_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8044151.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17554..18552
                     /gene="ftsY"
                     /locus_tag="IVY85_001123"
     CDS             17554..18552
                     /gene="ftsY"
                     /locus_tag="IVY85_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP8044152.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18739..21006
                     /gene="gshAB"
                     /locus_tag="IVY85_001124"
     CDS             18739..21006
                     /gene="gshAB"
                     /locus_tag="IVY85_001124"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP8044153.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(21095..21547)
                     /locus_tag="IVY85_001125"
     CDS             complement(21095..21547)
                     /locus_tag="IVY85_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP8044154.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            21697..22647
                     /locus_tag="IVY85_001126"
     CDS             21697..22647
                     /locus_tag="IVY85_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8044155.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            22640..23599
                     /locus_tag="IVY85_001127"
     CDS             22640..23599
                     /locus_tag="IVY85_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8044156.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            23596..24351
                     /locus_tag="IVY85_001128"
     CDS             23596..24351
                     /locus_tag="IVY85_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8044157.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            24374..25327
                     /locus_tag="IVY85_001129"
     CDS             24374..25327
                     /locus_tag="IVY85_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8044158.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(25971..26267)
                     /locus_tag="IVY85_001130"
     CDS             complement(25971..26267)
                     /locus_tag="IVY85_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP8044159.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            26622..26978
                     /locus_tag="IVY85_001131"
     CDS             26622..26978
                     /locus_tag="IVY85_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8044160.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            26988..27887
                     /locus_tag="IVY85_001132"
     CDS             26988..27887
                     /locus_tag="IVY85_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP8044161.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            28404..29135
                     /locus_tag="IVY85_001133"
     CDS             28404..29135
                     /locus_tag="IVY85_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8044162.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            29280..31307
                     /locus_tag="IVY85_001134"
     CDS             29280..31307
                     /locus_tag="IVY85_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP8044163.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            31415..32512
                     /locus_tag="IVY85_001135"
     CDS             31415..32512
                     /locus_tag="IVY85_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8044164.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            32819..>33264
                     /locus_tag="IVY85_001136"
     CDS             32819..>33264
                     /locus_tag="IVY85_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8044165.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT        11139 a         5762 c         7123 g         9240 t
ORIGIN      
        1 aaaaaaccgt tcactatctg aacggtccaa gtatcattca attaaacgcg ttcaactttt
       61 cctgatttca aagcacgagt tgacacccaa acttttttag gttttccgtc gaccataacg
      121 cgaacttttt gtaagttagg tttaacagta cgttttgttg agttcatagc gtgtgaacgg
      181 ttgtttccac tttttgtctt gcgacctgta aagtaacaaa cttttgccat ggtaatttcc
      241 tcctttgcct tgatttggag caatagaata attcagctca taccagacta acatatcata
      301 tagaatggaa atttgcaagt acattttcat agatacaatt gacaattgag aagaataatt
      361 gtaaattaaa caaggttata cctgaaattt tgcaaggttt ttgagtactt tgggtaactg
      421 ccttttattt caagttgcgc tatgataaaa tatgatagaa gaaaaatggg agttttttac
      481 ccgttaagga ggctcattca tggctgtaaa aattcaaacg tcagctggaa ccattgaaat
      541 taccaatgat gtaattgcaa cagttgttgg tggcgccgcg acagatgtgt tcggtgttgt
      601 aggaatggcc agcaaaaatc aaattaaaga taatattact gagattttgc gtaaagaaaa
      661 ttactcaaga ggagtagttg tccgccaaga agataatggt attgctgtag atgtttatac
      721 gattatcagc tatggtacga aaatctctga agtttcccgc aacgtccaag aaaaagtgaa
      781 atacaatcta gaggcattat taggcgtaac tgccaattca gttaatgtct ttgtacaagg
      841 tgttcgtgta ctgccagact aggcaggaga ctggctggta actcagcagt cgatctgaag
      901 gaggattatc taggtgagtg taacagaaat aagtgcaagc cagttccagg aaatggtcca
      961 ggcaggtgcg aatcgtctgc aagtaaatgc agaatacgtc aattcattaa atgtattccc
     1021 tgtgccagat ggcgatacgg gaacaaatat gaatctatct atgactagtg gagctaaggc
     1081 agtagtagat tcaacctcag aaaaagtagg agaattagca gcaatgcttt caaaaggatt
     1141 gctgatgggt gcgcgcggaa attccggcgt tattttgtct caattgtttc gtggattttc
     1201 taagcaaatt ccagatgttg tctcattaaa tgcttccgat cttgctgcag ccttcactca
     1261 tggagttgaa actgcgtata aagcagtaat gaaaccagta gaaggaacga ttttgacagt
     1321 agctagagaa gcagcgaaag ctggagaaaa gaaagcaaaa agtactgatg atgtcgttga
     1381 agtaatgaca gctgttgtaa aaggggcaaa gcgtgcttta gctaaaactc cagacttgct
     1441 tccagtccta aaagaagtcg gtgtcgtaga cagtggaggg caaggactgc tgtttgttta
     1501 tgagggcttt ttaagtgcat tgaatggaga attccaagcg gatgaaacat atgaaccttc
     1561 tccagcagaa atggatgata tggtcaatgc agaacatcat cgcagtgtcc aaggtcaaat
     1621 ggcgacagaa gatatccatt ttggttactg tacagaaatc atggtaaaaa tcggagaagg
     1681 acctacagtc gatagtacat tcgattacga agaattccgt aactatttag atggtatagg
     1741 agattcttta cttgtggtca atgatgacga gatcatcaaa gtccacgtgc atacagaaaa
     1801 cccaggagaa gtcttgaatt atggacaaaa attcggttca ttagtcaaaa tcaaagtcga
     1861 caacatgcgc ttgcagcatg agacgatttt ggaacacgat gaagaagtga acgagtttgc
     1921 gaaaaaagaa acaccacgta tcccgtatgg tatcgtagcg atcgctgctg gtaaaggcgt
     1981 ccaagagctg tttgaaagtt taggcgctaa ttatgttatc agtggtgggc aaacaatgaa
     2041 tccaagtacg gaagacattg ttaaagcgat tcaagaagta aacgcagata aagtgattgt
     2101 cttgccaaac aataagaaca tcttcatggc agctgatcaa gcagcagaag tagcagatat
     2161 cccagtagca gttgttcctt caagaacgat ttcccaagga ttgacagcta tgctttcatt
     2221 caatgatcaa gcttctttag aagaaaacaa agaaacgatg acagaaatgt tgtctagcgt
     2281 tgtgagcggt caagtcactc acgcgatcag agatacctca attgacggtg tagagatcaa
     2341 agaaggagac tttttgggca tgatcgatgg aaaaatcctg atctctaacc cagatatcac
     2401 agcaacagcg ttaagtacac tagaacaaat gatcaatgag gacacagaga tcgtaacgat
     2461 cttggcaggt gatgaaggaa cagttgaaca agcaaatgaa tttgctgaga aactaacaga
     2521 aaaacatcct gatttggaaa tcgagatcca ccaaggagat cagcctgtct atccttatct
     2581 attttctgct gaataatttc tgtgtcagaa tatctttcta acacagtatt cattcataaa
     2641 aggccttcgg ttagtgatca ggtatgatcg ctgaccaagt cctttttttg ttaagggagg
     2701 gaggaaaaat ttatgcgttc tttagatgat ccaatcacta tgctggcagg agtcggagaa
     2761 aaaagagcag ctagtcttgc cagcttgggc atccaaacga ttgaagactt actgacttat
     2821 tatcctttcc gctacgaaga tattcaggaa agaaatctaa acgagatcca agatcaagaa
     2881 aaggttactt tgaaaggaat cgtagtctcg cctcctgtga tgaatcgatt tggttataaa
     2941 aaaagccgat tgcaatttcg tatgatgcaa gaacatgatg tgttcaacgt ttcttttttt
     3001 aatcaacctt atctaaaaga aaaagtcatt ttatcggagg aaatcgccgt atacggtaaa
     3061 tgggatgcaa agcgtaaagc attgaacggg atgaagattt taggttctca aacaatggat
     3121 gacttttcgc cgatatatca cgtcaacaaa tcaattcgtc aaacgacatt agttgatcta
     3181 atcaaaaaag ggtttcaaga atacggcaca ttgattgaag aaaatctgcc aaacaacctt
     3241 gtggagaaat atcgactgct tgaccgagct tctgctgtaa gaagtatgca ttttccaaaa
     3301 gaccacgaag aaaaccatca agccaaacga cgtgtcgttt ttgaagagtt ctttttattt
     3361 caaatgcgtc ttcaaggatt gaaaaaagca gaaaaagcag aaacgaatgg tttggagatc
     3421 ctttatgatg tccaaaaatt gaaagaattt actcaaaagc taccttttga actgacaaat
     3481 gcacaaaaac gtgtaacgaa tgaaatctgt cgggatcttc gcagtccgca gcatatgcag
     3541 cgacttttgc agggagatgt aggtagcgga aaaacagtgg ttgccgcgat tgcgttatac
     3601 gctaccgtta cagcaggttt ccaaggtgcg ttgatggtgc ctacagaaat cttggcacaa
     3661 caacatatgg aaagcttgca acagctgttt gatcccaatg aagtaaccat cgcattgctt
     3721 acggggtcaa cgaaagcaaa agaacgtcgt gagctccttg aaaaactaga gcaaggggaa
     3781 attaacatcg tgatcggcac acatgcattg atccaagatg gagtcgaatt tcagaattta
     3841 ggtctagtta ttacggatga gcagcaccgt tttggcgtga accagcggaa agtacttcgt
     3901 gaaaaaggct ggcgaccaga tgtcttattt atgacagcga ctccgattcc tagaacatta
     3961 gctattacag catatggtga gatggatgta tctatcattg atgaacttcc agctggacgt
     4021 atcccgatcg aaacacgctg ggtaagaaca cctcaattgg acagtgtact ggattggacg
     4081 aaaaaagaac tggcaagagg acatcaaatg tatgtcatct gtccgctgat tgaagaatca
     4141 gaagcattag atgtaaaaaa tgcagtggaa atctatgaaa aacttcgtga actatttgca
     4201 ccaaaatatg aagttggctt gttacatggt aaaatgaaga atcaggaaaa agaagtaatc
     4261 atggaagcct tcaaagacaa ccagatgcag atattagttt cgactactgt aattgaagta
     4321 ggggtaaacg tgccgaacgc gaccgtcatg ctgatcatgg atgcggatcg atttggattg
     4381 gcacagctgc atcaacttcg tggacgtgtt ggacgcggaa gtgatgcttc ctactgtatc
     4441 ttagttgcta atcctaaaaa tgaactaggt gtggaacgga tgaaaatcat gacagagacc
     4501 accaatggtt ttgtactaag cgaaaaagat ctggaattac gtggtcctgg tgaagtattt
     4561 ggcttccgtc aatctggtct gcctcaattt gcgattgctg acttggtgac ggatggaaat
     4621 gttttggagg tagccagaga agaggctgca gccctttggc agataaagga ttggcagatg
     4681 cttcctgagt atcagccttt agcgcaaagc ctccaaatag aaaatcagga taatcgttat
     4741 ttcgattagc agactgtgct atactttata ctgatgacgg aaaaaggagt ggaaacaatg
     4801 aaaattgccg tagatgcaat gggcggagat cacgcacctc aagcaattgt agaaggcgtg
     4861 gctttagcgc agcaagattt tccagaagta gaattcttac tttacggaaa agaagcagaa
     4921 atcaagaaat acttaacaaa tgaaaaaaac atcacgatta tccatacaga cgaaaaaatc
     4981 aatagtgatg atgagccagt aaaagcaatt cgacgaaaaa agactgcatc aatggtcctt
     5041 gctgcacaag cagtgaaaaa cggtgaagca gatgctgtat tttcagcagg gaatacaggc
     5101 gcgctactag cagcagggtt gttcatcgta ggacgtatca aaaatatcga acgccctggc
     5161 ttgatgtcta ccttgccagt agtagggaaa gaaggtgccg gtttcgatat gctggatctt
     5221 ggcgctaacg cagaaaacaa accagaacat ttgttgcaat acggtatttt aggttctttt
     5281 tatgcacgta aagtccgagg tatcggacgt cctcgtgtcg cactcttaaa caacggaaca
     5341 gaagcaacaa aaggtagtga agtgacgaaa aaagcctatg aactccttca gaatgaaaca
     5401 agcttgaatt ttattggaaa cgtagaagct cgtgagctac tcaatggtgt ggctgatgta
     5461 gtggtcacag atggatttac aggcaacgct gttttgaaat ctattgaagg aacagcaatg
     5521 aatatgatga atctattaaa atctgctatt ctaaatgaag ggatcaaagg aaaaatgggc
     5581 gctatgcttt taaaagacgg cttacgttct ttgaaagcag aaatggatta ttccaaacat
     5641 ggcggtgccg ttttgtttgg cttgaaagca ccagtcatta aaactcatgg agcaacaggc
     5701 cccgatgctg ttcgctatac gatccgccaa atccacacaa tgcttgagac agatgtagtt
     5761 ggacaattag tcgaacaatt tgaaaaaagc gaagaatagt atttggatat ctgaagcaat
     5821 ccgttccatt gaaaaaaagg tggacattcc tgaaaaatgc cagtaaaatt agccatggtg
     5881 taggtaattg acaagtgtat gattcttgga ggtgaaaaag ttgactcgcg aagaagtatt
     5941 taacaaagta gcgaaaatta tttcaaatca ttttgagata gataccgata aagtgacgga
     6001 tgaattgaac atcaaagatg atttaaaagc tgattcaatt agtattatgg aattcgtttt
     6061 agagcttgaa gatgaattcg gtacggaaat ttcagatgaa gacgctgaac aaattgaaac
     6121 agtcggtggc gcagtagatt atatcagcag ccatcttaaa taatttcaaa taaaagcaaa
     6181 gaaggataaa aagctgcata cagcttttta tccttctttt tttaatcttt ttgacacgta
     6241 aaagtcattt ttctgataat tctggcgata aacagaaatt atgttgcaaa gtaaagcgat
     6301 tttctatata attattaata attcattaca caaagataag aataaccttt gttataaaac
     6361 gctttattct gtgtaatgtc cttttttatt aagaaaaact tccatttagg aagcagagag
     6421 gggtattcga ttgtccgata accaattatt agaagttcag aacctacata caggattccg
     6481 actcaaagat gactattacg atgcagtaga tgacgtttca tttactttag acaaaaacga
     6541 gattttagca atcgttgggg aatctgggtg cgggaaaagt accttggcta ctacgattat
     6601 gggattactt gatcctaata acacaaaaat tacaggtgag attttatata acgatctgaa
     6661 tttaatcgac ctgaacgaaa cgctctataa taaaatccgc ggaaatgata tcggaatgat
     6721 tttccaagat ccgttgtctg cattaaatcc tctcatgaga atcgaagacc agatcaaaga
     6781 aggactttcc tatcacacaa agatgaatgc agaacagcgt caagctcgtg cactagaatt
     6841 gctagaacaa gtagggattc ctaatcctgc gagagttgga cgccaatatc cccatgaact
     6901 atcaggcggt atgagacaac gtgtcatcat cgcaatcgca atcgcctgca aacctccaat
     6961 cgtcattgca gatgaaccga cgacagccct agatgtaacc atccaagcac agattcttga
     7021 tttactgaaa gaccttcagg aagaaacaaa aacagggatc atcttgatca ctcatgactt
     7081 aggagtcgta gcagaaatgg ctgatcgagt ggcagtgatg tatggcggac agttcgttga
     7141 agtagcaggt gtaaaagaac tgtttgatca tcctaagcat ccttatacac gctctttact
     7201 gaactctata ccacaagaag gaaatcatga tgcagaactc catgtgatcg aaggtgtcgt
     7261 tccttctctg aaaaacatgc ctcgtaaagg ttgtcggttt gcaccacgta ttccatggat
     7321 accagcaagc gatcatgaag aagagcctat ccttcatgaa gtcgcaccga accacttggt
     7381 acgttgctcc tgctataagc atttccactt tagagacgaa aaaggagagg tgtagatgac
     7441 agaattaatc acaatcaaag acctgaaagt tcattaccct attcgcagtg gattcttcaa
     7501 tcgagtaacg gatcatgtat atgctgtaga tggtgtggat tttattatcg aaaaaggaaa
     7561 gacatatggt ttagtgggag agtctggctc tggaaaatct actacgggaa aagccgttgt
     7621 gggattagaa aaggtaacag ccggacaaat catgtatgaa ggaaaagatg taacgaagcg
     7681 aagcaaccgc aaaaaaatgg gttataacaa ggatgtgcag atgatcttcc aagattcaat
     7741 gtccagtttg aatcctaaaa agcgagtgtt ggatatcatt gctgaaccca tccgaaattt
     7801 tgaacggtta agcgatcaag aagaaaagaa aaaggtaaaa ggactgctag atatcgttgg
     7861 gatgccggaa gatgcgctat ataaatatcc tcatgaattt tcaggcggac aacgtcaacg
     7921 tctaggagta gcccgtgctg ttgctaccaa tccaaaactg atcgtagccg atgaacctgt
     7981 atctgctctg gatctatctg ttcaagcgca agtattgaat ttcatgaaac gaatccagca
     8041 agaatacggc ctcagctatt tattcatttc acatgattta ggtgtagtaa aacacatgtg
     8101 cgacaatatc gcaattatgt acaaaggtcg atttgtagaa atcggtacaa gagaagacat
     8161 ttataatgac ccgcgacata tctatacgaa acgtctgctt tcagctattc cgcaaatcga
     8221 tgtagaaaat agggaaagcc acaaagaaaa tcgacgaaga gtagaacaag agtatatcca
     8281 aaaccaaaaa gaatactatg atgcgactgg tcgagtctac gatttgcgta cgatcacaac
     8341 tacacacaaa gtggcgttga aagatggagg tgcaagctaa tgtggaaaac gattctgcga
     8401 cgtgttttat tgatgatccc gcaagttatt attttaagtg ttctgatctt cttattagcc
     8461 caagcgatgc cgggggatcc atttaccggt ttgatcaatc caaatcagga tccggcagtg
     8521 atcgaacaga tgcgacaagc agctggcttg aatgacccat ggtatgtcca gtacttccgt
     8581 tggatcggaa atgctttgca aggcgatttt ggtcaaagtt tcttatacaa acgctctgtt
     8641 gctgacttga tcggcgaacg gatcatcaac actttatatc tatcgatctt gacagttgtc
     8701 cttacttatt tgattgctgt gcctctgggg atgcttgctg gccggtatca aaattcaatc
     8761 ttggataaga ttgtcgtaat ctacaacttc tttagttttg ctgttccttt atttatcttc
     8821 ggcttgatca tgctatttgt ttttggttat cgtttaggct ggttcccaac aagtggatca
     8881 caaacactag gtttgcaagg tggatttatc actcaatgga tagaccgttt gaaatatatt
     8941 ctgttaccat caattaccca agcatttttg gcgactgccg tcacaatcca atatctgcga
     9001 agtgaagtaa tcgattcgaa atcgctcgat tttgtcagaa cagcccgttc caaaggggta
     9061 ccgacgaata aagtattcag tcgacatatt ttccgaaatg cagcgttgcc gatggcttct
     9121 caaatggggt atgaaatcac ttcgttgatt gctggttctg tagttatcga aaagattttt
     9181 ggttatccgg gagtcggaaa actatttatt gattccatcg gccaacggga ctataccgtg
     9241 atcacatcct tagtattgat tttaggaatc gcgacgctag tggggacatt actttcagat
     9301 atcatcatga gtatcgttga cccgcgtatt cggattcaat aaaaccaatc agaaaggaga
     9361 atggatcgat gagtgaaaaa aacaatacag cagttcaaga aagtattcct cctatgggga
     9421 tacgaatgat tgctcgagaa tttaaaaaag ataaaattgc tattttttca ttaggtttgc
     9481 tagtggtttt attattagtg atctttatcg gtgcgatgct gaccgatcaa gacaaagtga
     9541 tgtatgttag tatcttcgac aaatacgctg caccaggaac agtttcgaca caaggacaaa
     9601 agttcattct gggagcagat gaaggcggac gtgatgtatt aggacaattg atcataggtg
     9661 ctagaaactc tgttacgatc ggatttgcga tcacgatcct tacttcgatc atcggggttg
     9721 gattagggat catctctggc tactacggag ggatcattga caacatcttg atgcggatcg
     9781 ttgattttat catgattcta ccgatcatgc tgatcattat cgtcttcgta tcgattattt
     9841 ccagatacaa tgtctggtcc tttatattca tcatgagtgc attttattgg gtagctaaag
     9901 cccgattatt ccgaagcaag acattatctg aagcaagaag agattatgtc agtgcctcaa
     9961 aaacaatggg aaccaatgat tttaaaatca tgttccgcga aatcatgccg aatctaagtt
    10021 cattaatcat tactaatttg acgatgaact ttgcggcaaa catcggtatc gagacaactt
    10081 tgactttctt aggtttcggc ttgccggcaa atgtacctag tttaggtaca ttgatcggtt
    10141 atgcaagtaa cggagaagtc ttagtgaatc ggcaatggat ctggttaccc gcatcaatcc
    10201 ttattttagt tttgatgttg agtatcaatt atgttggaca agcattcaag cgttctgcag
    10261 atgcgcgcca acggttaggc tagtatagaa gcgaaggggg aaataggaat gaagaaaacg
    10321 atttttggat ttgcagcgct gatgtctgct gtcgttcttg cagcatgcgg gggcggaggt
    10381 acagatacct ctggaaacaa ctctggaggc aacggcaata acaaagagga attaactttt
    10441 ccaattgcaa caaaaaataa tgatgatgca atcgaagacg gcgaattaga agctgcggtc
    10501 gcaacggaca gtcagtttaa aggattgttc cagtgggagt tttaccaaga ttcatatgat
    10561 gcagctttta tggctccttc tcatgaaccg ctatttgcaa acgatgacga tttcagaatc
    10621 acagacgacg gagctgcaac gcttgatcta gatgaagata cgaaaaaagc gacaatcaaa
    10681 gtaaaagaca acgtcaaatg gtcagatggt gaagatgtaa cggcggacga tgtgattttc
    10741 ccttacgaaa tcattgggaa taaagattac actggtatcc gttatgacga cactttccgt
    10801 aacatcgtag gtatggaaga atatcatgat ggaagtgccg acacaatctc cggaatcaaa
    10861 aaaatagacg acaaaacagt tgaaatcgaa tataaagaag taaacccaag catgcttcaa
    10921 tcgggtggtg gtatctggtc atatgcagca ccaaaacata cgttagaagg tgtagcaatc
    10981 aaagatatgg aatcaagcga tcaagtacgt aaaaatccag tgacttttgg cccttattac
    11041 atgagtaata tcgttgcagg agaatcagta gaatacttgc caaacgagca ctactggaat
    11101 ggtacgccta aattgaaaaa gatcacgatg aaatctgtac cgatggcaag tgcaactgaa
    11161 gcattgaact ctaaattata tgatatgatt ttccaaatgc caacagatac ttacgatacc
    11221 tataaagaaa ttgctggcta tacaaactta ggccgtgaac aaacttctta tacttatcta
    11281 ggattcaagt taggtaaatg gaatgcagat acaaagagtg tggaatacaa tccagattca
    11341 aaaatggctg ataaatcatt acgtcaagca atgggctatg cattagataa taatgcagta
    11401 ggtgaacgtt tctatgcagg tttgagaagc aatgcgactt cattgattcc tccagtattc
    11461 cagtcatacc atgatgatga gatgaaagga ttcacacaag atctagacaa agcgaaaaaa
    11521 ctattagatg atgctggcta caaagatgtg gatgacgatg gtttaagaga agacaaagat
    11581 ggaaataaac taacaatcaa ctttgcttcg atgtccggtg gggaaacagc gcaaccattg
    11641 gcagattact atatccaaca atggaaagaa attggtttgg atgtccaata cacaacaggt
    11701 cgtctgatcg aattcaattc tttctatgat aaattagaaa acgatgatcc tgaaattgat
    11761 gtttaccaag ctgcttggaa tacaggaact gatccaaatc caagtggact atggggacca
    11821 aatgctgcgt tcaactacac tcgttatgaa tcagaagaaa atacaaaatt gatcaacgat
    11881 atcagttcaa gcaaagcatt tgatgaagag taccaaaaag aagcatatca aaaatggcaa
    11941 caatatgcat ttgaagaagc atttgcgatt ccaacgttgt tccgtaatga agtattgcca
    12001 gtcaacgacc gtgtcaaaga ctgggattgg tcttatgatg taccaaaccc atgggcaaat
    12061 gtttctgtta cagcagattc acgttcataa tcactaaaaa tgaccgaaaa agggcgatgt
    12121 cctttttcgg ttttttgata tttttctgtt tatttctcta atattcaggt caaggcgctg
    12181 atttcatgac ttctccaata aacggtgttc aaagtccagt ttctcggtat taagtcaaat
    12241 ttatgcaaaa atatggaaag ccattttcac aaaatttttt cttcatactc gaaactaaac
    12301 gaatttttca caacttctcg tcaatcacta ggattattaa agctttactt accttttgac
    12361 cgaaccattt caaatttgac aaaaaaccag tataataagg aagtatcgaa aaaatatcca
    12421 taaaaggaat gtggaaaatg gacaaccagc tgacaaaaga actaaaagaa aactataaca
    12481 tcgtttttca cgatctaaat ttgcttgaac aagcttttac ccattcatcc tatgtgaatg
    12541 agcatcgcaa tctgcaatta tcggataatg agcgacttga atttttagga gatgccgttt
    12601 tagaactgat ggtttctcag tatttattcc gtttatatcc tcatttgcca gaaggaaaat
    12661 tgacaaaaac acgagctgcg atcgttcgcg aagacagctt gtcgaaattt gccaaagaat
    12721 gtcactttga tcaatatgtt ttgttgggca agggagaaga gaattcagga ggacgtacgc
    12781 gaccagcatt gctttgcgac ctattcgaag cttttttagg cgcgttatat cttgaccagg
    12841 gattcgacac gacccatgca ttcatcgaaa aagtgatttt tcctaaagtc aaagcaggtg
    12901 ctttttcaca tgagatggat cataaaacaa aactacaaga agtcttgcaa aaatcaggtg
    12961 atgtatcaat cgaataccga ttgatcaatg aagaaggtcc agctcatgaa cgagtatttt
    13021 ggatcgaagt ttatgtagat gatcaattga ttggcactgg acaagggaaa tcgaaaaaat
    13081 tagcagaaca agctgcggcg gaaaatgcat tggcagcact gtaaaagtaa gtgagggaaa
    13141 gctttgtatt tgaaacgaat tgaaatagcc ggttttaaat catttgcaga caagacagtc
    13201 attgattttg aaaatagcgt cacagcagtg gttggaccaa atggcagcgg aaaaagcaat
    13261 attacagagg ctatccgctg ggtattagga gaacaatctg ctaaaagtct gcgaggcgga
    13321 aaaatgccgg atatcatctt tgccggttcc gatacccgta agcagctgaa tattgcagaa
    13381 gtcacagtga ttttagacaa tagtgatcat tatctgccat tggagtacaa tgagatcagt
    13441 gtaacccgcc gttatcgacg gactggggaa agcgagtttt tcatcaataa acaaagctgc
    13501 agattaaaag atatccaaga attgttctta gattctggat taggaaaaga atcgttctca
    13561 atcatttctc aaggaaaagt agaagcgatt ttcagtagta aaccggaaga ccgtcgtggg
    13621 atctttgaag aagcagctgg tgtcttgaaa tacaagcagc ggaaaaagaa agcagaacag
    13681 aagctttttg aaacagaaga caacctcagc cgcgtccaag acatcatcca cgaattggaa
    13741 gaacagttaa ctcctttggc cgcccagagt gaggcagcaa aggagttttt acggctaaaa
    13801 gaaacactga ctcaaaccga tgtctctttg atggttgctg aaatcaagac agcaaaaaaa
    13861 gattgggaca acaaacaagc acagcttgcg aaattcaatc tagaattagg caaactatct
    13921 gagtcgatcc aagaacaaga atccattttg gcgaagcagc gtaaagaaaa tgcgcaagcc
    13981 gatcgtctga tcgaaaaaaa tcagcaagta ttattagatc tttcggaaaa actgaagcag
    14041 acagaaggac aaaaagatgt tttgcaggag cggacaaagc atacacaaaa aagctcgcaa
    14101 gaatatcaaa cttcattagc agaagcacaa aaaaaggtta aacattttga aaagcttcaa
    14161 gaaagcttga tgaaagcagc agcagaaaaa gaaacagaga tccaaaaagc tgaagcaaat
    14221 ctcataaaaa cacagcaaga attagaaaaa taccaaaaat caacgaaaga attattggca
    14281 gaattaagag atcaatatgt tgatttgatg caagaacaag cagctgtagg aaacgaactg
    14341 aagtatctag aacgtcaata tatacaagaa acagcaaaaa gcaagcagac gttagccaag
    14401 caatcggaag tagaagcatc agtagaccga ttgatcttac aaaaagaaga attgacacaa
    14461 aagcaggcgc agctaaaatc atctttaact gaaacaaaag agaagttaga aatgatccaa
    14521 caaaacggga aaaagttcca agaagcatta gcaaaagaac agcctaaaat gtatcaatta
    14581 atgaatcaag tccagcagct acgtgctcgt aagaaaagcc ttcaagaaat ccaagaaaac
    14641 tattttggtt tttaccaagg ggttcgattg gtcttgcagc ataaacagca gctttcgggt
    14701 attgtcggag cagtggctga actgatcgat gttcctgcag atttcacgtt agctatcgaa
    14761 gcagctttag gtggggcagc ccagcatgtc atcgttgaaa atgaaaaaga tgctcggcaa
    14821 gccatcacgt atttgaaaca gcaaagagga ggaagagcaa cattccttcc actgacgaca
    14881 atcaaaccaa gacaattacc ggctcatata ttgacacaag cagcagctgt agaagggttt
    14941 atagggattg ccagtgaaca agtcagttat cccgatcaaa tccaaactgt ggttcaaaac
    15001 ctgctaggaa cgatcctttt agccaaagac ttaactagtg ccaatgcaat cgcgcaaacg
    15061 atccgctatc aatatcgagt ggtttcctta gaaggtgatg tgatgaatgc cggaggatcc
    15121 atgacaggtg gagcgaataa acgaggaaac caaggcagtt tgtttgtaca aaatcaagaa
    15181 ttgaaacagt tgactagtga atttgaagaa gcagataaac aactccaagc acaagaaaaa
    15241 aaagttcagg agctgcaaca agaaacagct cgtttagccg aagaacaaga agttttgcgt
    15301 acacgaggcg aacaattacg ttttgaagaa caagaagcga caaatcagct acaaaacatc
    15361 ataaatgaac ttgaacgttt tgaaaaagaa aagcaaatct caacttttga gacacgtgaa
    15421 ctgcagcagt ttatcgaaga ttacgaaaag caaacgaacg aattgaaaga caaacaaaca
    15481 gatcttgaat cgcaacgaca aaaaatcgac gaagaaatca aatcgttaag tcaagaaagc
    15541 gaccagatgg aagccagaag agcacaagta caaagtcaaa aagcacaaga acaagcagat
    15601 cttgctgtct tgaaagagca gtttaatcat ctgcagatcc agctccgcgg tgcccgagtc
    15661 caaaaagcag aggcaaccga acgtcaagaa gcgatagaaa agcagttggc gacattgaca
    15721 gcagactttt ctgatcatga agtcacagaa gaaagtttgg aaaaacaaat taacgaatta
    15781 tctgctcagc gagaaacatt aaaagccgag ttggcaaaag ccaaagagca gcgagataga
    15841 aggcaaaagg agatcgataa gcttgaagca gtcttggcag agcgtaatcg tgagcaaaaa
    15901 gcacgactgt cggaacaatc caaattagaa gtccaaaaag accgagctga aatgatttta
    15961 gataatcatt tgacctatct gcagtcagaa tatcagctga catttgagaa agcttcccaa
    16021 gactatcaag aaacaaccga tatcgaagac agtagaacaa aggtttcatc cttaaaagaa
    16081 cagattgaaa aactgggacc agtaaactta aattcaatcg aacaatacga acaagtcagt
    16141 gaaagacata cattcttagc aacacagcga gatgatctgt tagcagcgaa aaaccaattg
    16201 tttgaaacga tggatgagat ggatgatgaa gtgcggacaa gattcaaaga agtatttgaa
    16261 gctatccgtc aagaattcaa aattgtcttt ccaaatatgt ttggcggcgg taaagctgaa
    16321 ttagtactga cggaaccaac tgatttgctg aaaacaggta ttgagattga agtacaacct
    16381 cctggcaaaa aactgcaaag tctaagtttg ctttctggag gagaacgagc acttacagcc
    16441 atcgcgttgc tcttttccat tatccgtgtc tgtcctgttc cgttttgtat cttagatgag
    16501 gtagaagcag cgttagatga agccaacgtg aaacgttttg gtcgttactt gagcgatttc
    16561 caagatgaca cacaatttat cgtagtcaca caccgcaaag gaacgatggt cgcagcagat
    16621 gtactgtacg gagtgacgat gcaggaatcc ggtgtatcaa aaatcgtttc tgtacggatg
    16681 gaagatatca atgaagaggc gaaaatcaac gaaacgaaag tgtaggaacg aaaatgatca
    16741 aattagtagc aatcgatttg gacggtacat tattaaatga acaaaagctg atttcagatg
    16801 agaataaaca agcattagca caagcaaaac agcaaggggt caagatcgtt ctatgtacag
    16861 gacgcccctt agctgcgatg gcgcattatc tgcaagaatt aggactggta gatgaaggtg
    16921 actacagcat cactttcaac ggcggtttgg tacaaaaaaa tgatactggt gaaatcatgg
    16981 aaaagaaagt gatggaagta tcagatatcc aacgcttata caaactggct cagcaattgg
    17041 atctgccact agatgttttg tctgacaatg tggttttaca gctgcctagt gccccaagga
    17101 agcaatcctt gtacaatgtg ttgaataagc tgctgcaatt ccaaccagcg gatataagcg
    17161 atattacaga tgaatttgtg cttaataagg cagtaatcgc ttatgaccaa gaagaattag
    17221 atcctaaaat caaagaaata ccagcagaat tccatgaaat gtatgagatc atcaaaacaa
    17281 gaagtatgct tttggaattt atgccaaaag gtgttacaaa agcttatggt atttctttat
    17341 tggcaaaaga tcttggtttg aaacaagaag aaatcatggc gatcggtgat gaggaaaatg
    17401 atttaccgat gatccaatat gctggactag gcgtagcgat ggagaatgct gtgcctttcg
    17461 tcaaagaagc agcagatttc gtgacagcca gcaatgaaga aaatggcgta gccaaagtga
    17521 tccaaaaatt tgtattagag tagggggagt caaatgggat tttttgataa aatcaagcgt
    17581 gcttttactg gcgaagagaa aacagaagaa gttgaagtac aggagaagta cgacaagggt
    17641 ttagaaaaga cgcgtaaaac atttggtgaa cggatgaatg agttgtttgc caattttcga
    17701 acagtcgatg aagacttttt tgaagaatta gaagaaacac ttatcggtgc agatgtaggt
    17761 tttgaaacag cgatacgaat caccgaagca ttacgtcaag aagtgaaact cagaaatgcg
    17821 aaaaaatcgg cagaagtcca aaatgcgatc atcgagaaac ttgtggatct ctatgaagcg
    17881 gaaggaatca atgaagtaaa tgaactaaat atccaagaag acggactaac agttattttg
    17941 tttgtcggtg ttaatggtgt agggaaaaca acaagtattg gtaaacttgc ccatcagttc
    18001 aaaaacgaag gtaaaaaagt attgatggca gcagcagata ccttccgcgc tggagcaatc
    18061 gaccagttag ttgtctgggg cgaacgcgct ggtgttgaag ttgttcgtgg caatgcagga
    18121 ggcgatccag cagctgtcgt attcgatgct ttggaaagag caaagacaga gcaggcagat
    18181 gttttactag ttgatactgc aggccgactg caaaataaag tgaacttgat gaacgaatta
    18241 gaaaaaataa aacgagtaat ccagcgggaa ttaccagaag caccccaaga aacacttctt
    18301 gtattagatg caaccactgg tcaaaacgcg atggttcaag caaaacaatt caaagaaaca
    18361 acagatgtga ccggtcttgt tttgacaaaa ttagatggta cagcaaaggg cggtatcgta
    18421 cttgctatcc gtaatgaatt gcatttacca gtgaaattgg tcggtcttgg tgaaggaatc
    18481 gatgacttgg agccgtttga tccaaatgat tttgtcgttg gattgttcaa aggattacta
    18541 aaagaagaat aacccatgtc taaaaagcag gagctgtgac aaaagttgca gctccttgtt
    18601 tgaatgattg tttttaacat agctgtgttt ttgatgcctt catttttata cttatttgta
    18661 agcggttctc aaacaatttc gttctttttc ttagactttt tgattataat tagattcata
    18721 tgaattggag gtcacttaat gatgaatttt aagcaattat tattgcatgt caatgcgcgc
    18781 ccttttatcg accaagctcg atttggtata gaacgggaag gacagcgggt tgatcttgca
    18841 ggaaatctag caaaaaccga tcatccagca atctttggcg atcgatccta tcacccctat
    18901 atccaaacag attttagcga aacacaaaca gagatgatca cccctgttac cgattctatt
    18961 cccgaattat ttcagtatct cgctgctgtt tatgatgtga ctgctcgttc tataccgaaa
    19021 gaagagatga tctggccatt aagtatgcca cctgccttac cggaaaaaga cgaagagatc
    19081 attattgcaa aattaaaaaa tttcgaagat gtcttgtatc gacgttattt agcaaaaaaa
    19141 tacgggaagc gaaaacagat ggtaagcggt attcatttca attttgaatt tggcgatgag
    19201 ttgctaagaa cattgttcag ccatcaggaa gaatttcaag atttttccga attcaaaaca
    19261 gaactttatt tgaaaacagc gagaaacttt atgcgctatc gctggatgat cacttattta
    19321 ttcggtgctt caccaatgag tgagaagaac tactttttag atgaatcaca tccgcaagaa
    19381 cctgttcgca gtattcgcaa cagtgcgctg ggctatacga atcatccaaa tgtgaaagtt
    19441 tcctatgctt ctatgaaaca gtatttagca gatatcgagc gaatgatcga agaagggaaa
    19501 ctttcggagg agaaagaatt ttatacaccg cttcgtttcc gaggcggaaa gaaagtcgca
    19561 gatttagcaa caacaggtgt tcgttatatt gaattgcgga atatcgattt gaatccttat
    19621 gcaagattgg gtatcaatcc agagcaagtt cggttcttac aattattcct gatgtacatg
    19681 ttatggacag aagaaaaaga agactgtgac caatgggtag cagaaggtac gactcgaaat
    19741 aacaaagtag cactagaaca accatcagat caaacagaat ttcatcaaga aggtagagaa
    19801 attcttgaag gaatgaagca aatgctggtt gaattggatt ggttggattc tctttattta
    19861 gtagaggaag cattgactca aatggatcat cctgaacaaa cgttagcagc gaaactctat
    19921 caagaagcac agctatcgag tcagcaagaa gttgctgtgg cattgggaca tcaatattat
    19981 aaagaaagcc atgaacgtcc ctatcagttg gccggttttc gcgaaatgga actttctact
    20041 cagatattta tgttcgatgc catccaaaaa ggtgtccaag tcaaagtatt agatgagtcg
    20101 gatcaatttt tgcgtctgca attccaagac catgtagaat atgtgaaaaa tgcaaacatg
    20161 acaagtaagg acagctatat cgtcccactt atcatggaaa acaaaacagt aacgaaaaaa
    20221 gtcttaaaag aagctgggtt ccgggtacca ggaggcgcag aattttcatc catggaagaa
    20281 gcggtaaaag cttatccgag atttgccgag caggcatttg tgatcaaacc taaatcaacc
    20341 aattacgggt taggtatcac gattttcaag gaaggagcta gtctggaaga ttatcaagca
    20401 ggtttagcca ttgcttttcg tgaagacagt tcggttttag tagaagaatt tatgccggga
    20461 acggaatatc gtttctttgt gatcgacgga gaagtccaag ccatcatgtt gcgagtccct
    20521 gctaatgtta ttggtgacag tatccgaaca gtgaaagaac ttgttgaaga aaaaaatagt
    20581 gaccctttgc gtggaacgaa tcatcgtgca cctttagaat tgatccaatt aggtgaatta
    20641 gaacaattga tgttgaaaga acaagggttg acgatcgaat cagttcccca agccaatcaa
    20701 attgtttatc taagggagaa ctcaaatatc agtacaggtg gtgattcgat cgatatgact
    20761 gatgaatttt ctgaagctta caaaaaaatc gccgtttctg cggtagaagc actaggagct
    20821 aagattagtg gtatcgattt gattatacca gataaagaaa tcgatcctac aactgataaa
    20881 aaagcatatg ggatcatcga agcaaatttc aatccagcca tgcatatgca tgtttatcct
    20941 tttgcaggaa aaggcagaag attgacgatg aatgtattga aattgttata tcctgaagta
    21001 ttttaaaaga aaaagctttt gacagattgt cctatttgat gtcaatctga tcaaaagctt
    21061 tttttgcatg taaaaattta tgtgtttttt cgttttaaaa taaacgatcc cagaagcttt
    21121 ttttcttttt tggtttttct ttttcagctg gcgaagagtt ttcttctggg tatttttttt
    21181 cgatatcaat cacaggttca tttactgctt cttttgctgt taagagcaat ccgaattcct
    21241 caggatttgc agtgacgtaa tcgttgacta cagtgaaacg gctattgttt ttcgttgcta
    21301 gctggatata ttggttttgg acagaaaggg gtagagcccc attcaagagt atggaagcat
    21361 ctggatgttt tttgagttct tttaaaaagt tttctttgtt ttttggttgg atcatttcag
    21421 caacagtcat actaagatag cagcgttccc tgaatgtccc taaatattta tgctgttctt
    21481 cagggttcac taaaggtgta ccataaagtc ccttatccaa atggcttttt acatcatctt
    21541 gtgccataaa aaataccctc cttcttctct ttgtctttct tacgattata gcatattccc
    21601 aataatacct aaaacatatg aaaaagcggt tgcttttgtt caacgattca cttaaaataa
    21661 gaatgaaaat cattctcaat tagaaaggtg gattggatgc gctacggacg actagtagca
    21721 ttattagtta ttttaagtag tatttcattg ttcatcggag tatcgaatgt acaattgtca
    21781 gaactgctcc agttagatgg gcaaagctta caaatcttgc tagtgactcg tttgccacga
    21841 acgatttgtt tgatccttgt gggagcgacc agtagtattt gtgggttgat catgcaacat
    21901 ttgacccaaa ataaatttgt ttcaccaacg actgcgggca cgatggatag tgcgcgttta
    21961 ggtattttag tggctatgat tttcctacct ggtgcatcct tgattgtccg atccttgaca
    22021 gcattttgct ttgcttttgc aggaacattg ctttttttat cccttacgcg attgttcctc
    22081 cagaaaaatc aattgatgct tccgttgatt ggagtcatgt tagggaatat cattggttca
    22141 gttgctactt tttttgctta tcaattccag ttggtacaaa atatgacttc atggcttcaa
    22201 gggaattttg cgactgtgat acgaggaaat tatgagttat tgtatgctgt tgttccattg
    22261 ttgctcttgg tttggctatt tgcttaccga tttacgatca ttggtctggg cgaatcgttt
    22321 gcagtaaatc tgggaataga ttaccagaag atgcagtttt taggcattgg cttagtatca
    22381 ttggctagtg cagtcatgtt gatcatggta ggaagcattc cttttatcgg agtgatcatt
    22441 ccgaatcttg tctctagaat atatggagat caagtgcaca aaacaattgg cgtaactgct
    22501 ttagcaggta gtttgttctt agtattttgc gatcttgtcg ctcgcgtgtt gatttttcct
    22561 tatgaaatac cagtcagtgt gatcgttgga atcatcggtg gggctatctt cctttatctt
    22621 ctgataaggg gacgacgata tgcttaaaaa aaagccatct atttttattg gtatgcttgt
    22681 tcttgtttta tttgttacag gaatgtactt gacgatccat acatatggaa actggcattt
    22741 tgcatgtgca ttgaggagta agaaaatttt tgcatttatc cttgtctcgc tagctgtcag
    22801 cagtgccaca atcagctttc agacaattac gcataaccag tttttgactc cttcagtatt
    22861 gggacttgag tcgttatatg tccttgtcca aacggtcctt ttcttcttta tcggaggagt
    22921 gacgatgctt cagcagacag gtacagtttt tttcttattg aacgttgggc tgatggtcgg
    22981 gctgagtcta ttgttatttt tctttctttt gaaaaaaatg agagcgaatc tttttttatt
    23041 actgatgtca gggatgattt taggtacgct ttttggtagt gtcagtacct ttttacaagt
    23101 catcatggat ccgaatgaat atgatttgct tcaaggcaga ttatttgcaa gctttggcaa
    23161 tatccagagt cagtatcttt tgcctgcttc tttgatcatt atcggctgct ggtacctgtt
    23221 atttcgatat cgacatgtgc tagacgtttt ccatctcgga aatgaacaag cagtaaacct
    23281 cgggatagac acagatcgtt ttgaattttt gattttatgt tgtgtcagtc ttttgacagg
    23341 gacatcaacg gctcttgtgg gtccagttac ttttttaggg tttattgtcg caaatatcag
    23401 ttatcgtgtc ttttctactt atcgtcatgg tcagctattt atcggaagct ttctggtagc
    23461 tatgctgttt ctgacaggag gacagctcgc agtagagcag attttcggct ggaatacaac
    23521 agtgagtgtc gtcgttgaat ttgtcggagg aatttatttt gtcacaaaat tgattcgcga
    23581 aaggaagagt atcctatgat tgatttacaa aaggtcaata aactgtacca tggaaaatca
    23641 gtggttaaag atgtacagtt aacgattcct gaaaagaagt tgactgcttt tatcggtcca
    23701 aatggtgctg gaaagtcaac ggtgttgtcg atgatcagtc gtttgatacc aaaagataca
    23761 ggtgaaatct atttagacca taacgaagta aaggcttgga aatccaatga attagccaaa
    23821 aaattatcga tcctacgaca atcgaatgaa ataaatgtcc aaatcaccgt gaaagaatta
    23881 gtagaatttg gacgatttcc ttatacgaaa ggaaaattga cgacaaaaga cgaggaaatc
    23941 gttcaggaat cactggaaca ccttgggtta gaagaaatag cggatcaatc gattgacact
    24001 ctttcaggcg gtcaactcca acgtgcgtat attgcaatgg tgctagcgca agacacggat
    24061 tatattttac tggatgaacc tttgaacaat ctagatatga attttgcggt tcaaatcatg
    24121 cagattttaa aaaatttagt acaagaaatc gggaaaacaa ttgttattgt tcttcatgat
    24181 atcaactttg ctgcaagtta tgcagacgaa attgttgcaa tgaaagatgg acagttattt
    24241 acgcaaggaa cgacccaaga aatgataaaa aaagatgtat tggatgaatt atacgaaatg
    24301 gacatacgga tttgtgagat tgaaggccgc agattctgtc tttatttcta aaactaacat
    24361 taggagagaa gagatgaaaa aattagtagt aggtatcttg attctagcat catttggttt
    24421 agcagcatgt ggaaatagca acaacaccag tcaggcagac acaaaaaaaa gcagcacaca
    24481 aactgaaact acattgacga taacagatag taatggagac caaatcgagg tgccaaataa
    24541 tccagaaaaa gtcgtcgtat tcgacaatgg ttctttagat acgatggatg ctcttggtgt
    24601 tggagataag gtagttggag cagctacaag cagccttcct gagtatcttt catcctataa
    24661 aaaagtagaa tcagcaggtg gaataaaaga accagactta gaaaaaatta atcagcttca
    24721 gccagatcta atcatcattt ctggccgaca aagagatttt caaaaagatc taagtcaaat
    24781 tgctccgacc attttccttt ctttagatgc gaaaaatcca tgggaatctt tccagcaaaa
    24841 tgtcactgct ttaggggaga tctttggtaa gcaagaagaa gctaaaactc aattagagga
    24901 attatctagt gcaatcgatc agacgaaaaa gaaagcagaa gctactgaca aaaaagcact
    24961 agtcacactt gtgaatgaag ggcaattatc agcctacgga tctggttcta gattcggttt
    25021 catccatgat ttatttggct ttgaacaagc agatgatcaa atcgaagcat ccactcacgg
    25081 tcaaagtgtt tcttatgaat atgtgctgga aaaaaatccg gatatcttat ttgtagtaga
    25141 cagaacaaaa gctattggtg gagacgacag caaagatgat atttctgcca atgaactggt
    25201 agcacaaacg aatgctggta aaaatcagca gatcatctcc cttgaaccag atgtctggta
    25261 tttaagcggg ggcggtttag aatcaatgaa actgatgatc gaagatgtga atcaagcatt
    25321 taaatagcat aaataacaga ggattaaact ctcatactat gtaacaataa tgtttcttac
    25381 attcttaaat agcaagagcc tatcctcaaa gaagttatca aaaaccatcc gtaaaatcgt
    25441 attgatgatt ttacggatgg tttttattta tttgagaaat cttttttcaa cgtgaattca
    25501 ttatctacat catacgggtt gattctagaa tggggagtgg caaaaaatag tgtgatccat
    25561 tttttcagca tcaagatcgc ctcctttgat ttgaatcaaa ggtacaaagc gaaacttaga
    25621 taaaacttaa atttagatat ttttattttg taccgatctc ttctttaaac gatgttccat
    25681 ctgctgcttc tcggcaataa gtccactaga ctagaaaata tgagaagcta ttttctgtcc
    25741 agcgttctta ttactcaaaa caaatcgcag ttgtcacaat ctcttctaaa cgatgttcca
    25801 tctgctgctt ctcggcaata agcccactag actagaaaat atgagaagct attttctgtc
    25861 cagcgttctt attactcaaa gcaaatcgca gttgtcacaa tctcttctaa acaatgttca
    25921 atcagctgac cttcggtatt agctcaaagc actgatttca caacctctat ttaggcatgt
    25981 gtctcagcaa gcggttgata gttgatgatc tgcatttttc gataaaaatc ggcgggaagc
    26041 ttactccacc gttttagtaa ttgatagaaa taactctctt ctgctttgat tttatactcg
    26101 cttaatatca atccaaaact attcgttttt tttgaagcta attttttata aatatccaat
    26161 gctttggttt tatcttcatt gacaaagagt gtttttactt caatataaga accctcgata
    26221 ttttgtgttt cttcgataaa atagatgttt tttatatatt ccttcatagt gattcctcca
    26281 aagaagtctt ttatttgata agtttactct accgaagagt tgttaataat ccgtgaacaa
    26341 aaagtaattc tttttccaat agttgttaaa ggttttttat tttttatagt attttttgtt
    26401 atttaagaaa ggaataaggt taaacaaata acaggttgct ttttgggtct ttttccgttc
    26461 aagaaactga acgaagaaaa gggaaagttg tctcttttgt gataagagaa atcataataa
    26521 acgaatgact tactttccaa taaattgaga acagtcagaa ccagacaaat tagttgcatc
    26581 tgatgaataa agtgattatt ctatgacatg aggtgataca gatgaaatta gaagtgacaa
    26641 aagaagcaca agaagtattg aagaataaat tagaagcaga cgatcaactt ttattagata
    26701 tcgaaaacgg tgacggtcca tttgcagaaa gccaagtcag ttgccaattg gatacggcat
    26761 ttcgattgat cattgtcaaa aaagatacac aaacagatct aagtctttat tctgtagtgg
    26821 ctgatacacc agttggacca atcaaaatca aagacagtgc aatactttac atggatgatc
    26881 catccacact tgctttagaa ccaacttata atagtttaca attaaaagga ccaagcggct
    26941 tgagaaaagg caacttgcaa gttgttcgaa aagactagag ggataaaatg ggaaaattgg
    27001 cgatcatcag cgacctacat gtggacatca ataaattcgg cgaatcagag ctgatgcagc
    27061 tttggcaagt tttgcaaaag cacaagatca ctcggcttca tctagccgga gatacggcta
    27121 ataaagtaga ccgttgccta gctgttgtcg aatttttcgt caataaagga ttatctacga
    27181 catttcattt cggcaatcac gagttggcag atgtgcccaa tgaacagatg atggaccatt
    27241 ttccagaccc acatttttta aatgaacggt atattgcttt aaatgagcag accgttcttt
    27301 tagggatgaa tggctggtac gactatcagt tttccgaatt gaaagattac gaggcaatcg
    27361 tacgaatgaa gcgtttgtat tggtatgatc ggttcatcca gcgtgggaaa acagatctcg
    27421 aggtcaatgc agaaatgctt gcagctgcaa aaaatttgct agaccagctg gaagcaaaaa
    27481 aacttgaggt gattttatcc actcatttcg ttcctaaacg ggcatttatc atgtatcaat
    27541 ctgctcctta tgagcgttgg aacaaattaa atgcattttt aggatcagaa tcattcggag
    27601 aattacttga ccgttattca aatatcaaac aagtagtttt tggccatacg catcgtcgct
    27661 ttgaagataa atccatcggt cacacgattt acagctgcag accttttggt tattattatg
    27721 aatggcagct gacccgagat tttgtcttga aagaacagtt gctagacagc tatgatccta
    27781 tgaaattacg tgtcctcttg cgtgcccatc acccagcttt tcaggcctat caaacacaac
    27841 atttgcaaga ggaatttgaa caagcgatga caatcgttcc gtattgagac agcattagta
    27901 atgtgtaaaa gaaaatacct tgtaaaacat aaggataagt aagagcagta gatccattta
    27961 tcgtggatat gctgcttttt ttgcggaaaa taatcagcga atgtttattt gatcggatga
    28021 tttgtaactc gcagagtact agatgcaagc gtgtattctt tttttgaacg ataatcacaa
    28081 aaaaaaatat aatatacatt ttttatttaa ttataattaa tattgattga agtctaggca
    28141 gtcaagtcac attttgatag ataggagggg gacttacgaa ttattttaaa ggctatgaca
    28201 gctgtatttc tagattgtgt ttgcatcaag ttgcaataaa aactagaaat caaatcgggc
    28261 tgataatcat ttataactaa tcgatacata ggaccaaata taggtctatg ttcttttatt
    28321 tcataagtat cgtaaaacag aattttgcta tggattaatc caatcgactg atatgtttag
    28381 taaataaaga aagaggaatg tcaatgatga aaaaagaaat cgtattaaca gcagcaatga
    28441 tgttattcag catggtagca agtacgactt ttgtttctgc aactgaagta tacccaaagg
    28501 aatataacac agaaggaacg attacatttg aagcaggaga tgagggtgta actcctcctg
    28561 ttgatccaga gaatcctgat cccaataaac ctgtcgatcc atcagatcct ccaagccctg
    28621 gaacaggagg ggcattatcg attgattatg gatcaaaatt caaatttggg actcaaaaaa
    28681 tttctactgc ggataaaacg tattatgcag ctgctgatgt gatgaacgat gggtctagaa
    28741 aaccgacata tgtccaagtg acagatagga gaagtacctt atctgggtgg aaattaagtg
    28801 tctctcaacc agaacagttt aaaactgcca gtggggatga actagttgga gcacagctga
    28861 aatttacgaa agggcaggct gtttctttgg ttgatccaac atatacgccg caaacggtga
    28921 attctgaatt aacgttgaca ccgggaggaa acaatacgtt agcaattaat gctaaatcag
    28981 gaacaggtgt ggggacttgg gtctatcgat ttggtgcaaa tgaaaatgaa aatcaagatg
    29041 cggtacagct ttcggtaccc ggtaaatctg tcaagttagc gcaacaatat tccactaaat
    29101 tagtttggac attagaagat acacctaaca actagtttac tgaaaagaac tgttaaaaat
    29161 aaatacgtag tcatattgtc ttgcgaggta atcgagcaaa aattgtcaaa ggctaggcta
    29221 aatttgtttg gatacgaata gttcatctag gaatttaacg atcaggagga aaagatatat
    29281 tgaaaaagag gaggatcgtg ctcattttaa gcagtctctt agtgactaat atctttccga
    29341 tcatagggta ctctttggaa ttgaatcagt tggagcatgg aaatagaaga gacgaagaaa
    29401 gcaaactcac ttcgacattc gacagtttaa tagaatccaa ccagacacaa gaaagcagtg
    29461 aggatttaag tatttcgagt agtggagaga ccactgacaa cgtagaagct gagggttcgg
    29521 acaaacaagg actagaagat gcaccaattt ctgcggttga gctgcctgac gatcccaata
    29581 ttatttctat cgataaaatt tttcaaacac ctatcggtac tagcacgtcc atattggaaa
    29641 atgggaagtt actgcagttg aacccagcag aaaggtctca aagaggagct atttggtcaa
    29701 ataagaagat tagtttactc tcggatttta catttaaatc ttatttatac ttaggaaatc
    29761 aacgagatag agcgggagat ggcatgacct ttacattgac tggcgatcct agaatggcta
    29821 gtaatcccag tcaagttatt ggatcgccag gtatgggaat tggctcatat tctactcgtt
    29881 ctggccaacc ttacattgcg aatgcgctct cgattgagtt tgatacgtat aaaaatagtg
    29941 gttcttctga tcgaatggat cgagagataa ctgctgataa tagagggcat ggacatgtcg
    30001 cttttgtcac gcctaaacca aataataata attattctgg agagcatact ggcgtgacgg
    30061 tagctcctac atttctttct aatggcacat ggcgcttgtt aataattaaa tgggacgcgg
    30121 agcaaagaag tcttacttat gatttggaag gtgtcggtcg ctcttctcgg attatacctg
    30181 atctcattag gcagtttggt tctacagaag taaattgggg gtttacttct tcaacaggta
    30241 ctttttttca agaaaatgct gtcgctgtca cgcgacttcc atcgaatgtc acatcaacag
    30301 ctgatgtaaa agtcaacgat agtgattatg gggccatcgc tgaagctgga ggaaacgata
    30361 agataactat cagaaataca ttgaaggtcg agggggattt tcttgaatat aagaaaatgc
    30421 agactagtat cgacctccca caagaattaa ctattcccga gcatatcttg atcaatggaa
    30481 atcaaattga tgccaaagat atcttgataa ccggaaatcg agtcacgcta aatctgacag
    30541 attattttat tgctggagat acgttaataa tcgaattgga gacaactttg caggaagtaa
    30601 ttcctgaaaa agtactcact atgcattttg aataccttga aaacgggaat ttgattgagc
    30661 aatcgaatga aataaaccta aagatcgcaa agctgaagga gatagatatc catgtttatt
    30721 ataaagataa agacagtcag attgatattg ctgaaatgaa aacaatcact gggaaaatag
    30781 gagagaagta taaagaaact cctattgata ttgaagggta tgtttttgac tcggattcag
    30841 cgaatacaga aggcatcttc tccgaaaata cagaggatat ctatttttat tacagaaaag
    30901 gcaaacttta tctctcaact gctcctcaat atgtcaattt tggaaaacat aaaatatcca
    30961 gtagaccatt aagtgaattc ggacagtttt caggtgggtt gaaaattata gatgaacggg
    31021 cttttggaag ttggaaatta caattgaaac aagagaaagt tcttacaaat ggagattttg
    31081 agcttccaga agctctttcc tttgttacct ctgaaaatac aacggttata gggacatccg
    31141 ctgttacgat tttcgaaagt gatcaaaagg gtgaaagcga tttgtcctct cttttagatt
    31201 ctacagctca tcgtggaatc agaataaata ttcctgtaga aaaccaaaga atcggaacct
    31261 ttgaggggaa actatcttgg atacttgcag atgtcccagg aaattaacag gaagagaaga
    31321 atataacaaa attattgagt ttcaagaagg gcacaggtac cataggctct tgtgtcccac
    31381 agagacaaga ttttgttata gtcttctctt acatatgatt aaaggagcga ttttcttgag
    31441 accaaaacaa caattattta taacactcct agttatttta ggggcacttt ttaacaatat
    31501 atcagtgtca gctagtgcat ttaatttttc agtgacgccg ataacttcag agaatcaaat
    31561 cgacaagaga aaaacttact tcgatcttca acttgatccg gatcaggaag tagaagtaaa
    31621 agcggaattg agaaatgata cagaaaaaga agtgaaaatc gatatttctg tgaatagcgc
    31681 aaccaccaat tcaaatgtga tggttgaata cggaaaaaac gagattgaaa aagacatgag
    31741 cctgatcttt gatctggtag attatgtgag ctatccacag atagtgacgc taaaacctaa
    31801 aagtgtccaa accgttgctt ttcatgtcag gatgccgaat gagcgatttg atggtgtatt
    31861 ggctggtggg atcacattcc aagaaatcca gacggaaaag gaccaaacag aaactaagga
    31921 tcaaagctta tcgattgaga atgaatacac ctatattatt gctttgttga tgcaacagac
    31981 actgaacgaa gttgctccga accttactct tcatgaagtt aaaccagatc aaatcaatgc
    32041 aagaaatgtc atcttagcaa acgttcaaaa tgatcaaaaa acctatatca atcaagtagt
    32101 tattgaaaca aaaattacaa aaaaaggaca ttctgaagtt ttatatcaag aggaaaaaga
    32161 aggattgcag attgcgccga atacaaattt ttcttttcca actgctttaa atggtcaacc
    32221 gctaacccca ggtgaatatc accttacaat gactgtttta ggaaatgaaa atgaaaatgg
    32281 aaaattttcg agaaaaaaag gaaatactac gatcaactat accaatcaat gggtatttga
    32341 aaaagatttt acaatagacg gaaaagtagc caaggaattg aacacgacgg atgttacgat
    32401 taaaaaagac aataattgga tttatatctt agtagcttca cttttcttat tgtttatttt
    32461 attatgcata cttttgtttg ttttgagaaa aaaagaagag aaagaaaagt aaggttaaga
    32521 taacaaatag taaattttta ttattgtagg atggttgggc tctttttata acaatataaa
    32581 atataccatt ttattaataa aaatgcatac tagtaaatga tataattcta ataacaggtt
    32641 tgatcgcgag aaaggccaaa taaaacgctc tccaaaatgt taaaaaaaag acaggacgat
    32701 gtatattata caaacaagaa tcgctgacaa cagtgactcg gatccaggaa atcagtttta
    32761 tccccagcta gttggtgaat gcatggaaag taatatacgt agaaagagct gatgactaat
    32821 gaattatgag ttttttttaa gcaaagaaga tcacagaagt tattcattac ttaaatattt
    32881 agaagcaagc tcaaatttat cagagtcaat atcgaatgtt caagaagagt tgtcgctatc
    32941 gacatttctc ttgaaaaaaa cgatcgataa gctgaaacat gatttacaaa agttcgatct
    33001 agagaaaaat ttgcgagtaa ccgtcagtga attggatata tgtctggaaa ttaatggaaa
    33061 atgttctagc aaagctctgt tatctcgtta tataacggat tctttatcta tgaaaatggt
    33121 attggctttc tttcaggaaa aatatatctc agtagaaaat tttgctaaac agcatcacgt
    33181 gagttattct gtggcttata aagtattaca aagactaaaa agaaatctaa aaaaatatca
    33241 aatttttttt gaaaagagaa agtt
//
LOCUS       DACCYH010000026        32191 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.026, whole genome shotgun sequence.
ACCESSION   DACCYH010000026 DACCYH010000000
VERSION     DACCYH010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.026"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(129..509)
                     /gene="yajC"
                     /locus_tag="IVY85_001137"
     CDS             complement(129..509)
                     /gene="yajC"
                     /locus_tag="IVY85_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP8044166.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(606..1751)
                     /gene="tgt"
                     /locus_tag="IVY85_001138"
     CDS             complement(606..1751)
                     /gene="tgt"
                     /locus_tag="IVY85_001138"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP8044167.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1969..2667
                     /locus_tag="IVY85_001139"
     CDS             1969..2667
                     /locus_tag="IVY85_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8044168.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2835..5474)
                     /locus_tag="IVY85_001140"
     CDS             complement(2835..5474)
                     /locus_tag="IVY85_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8044169.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6093..7124)
                     /gene="queA"
                     /locus_tag="IVY85_001141"
     CDS             complement(6093..7124)
                     /gene="queA"
                     /locus_tag="IVY85_001141"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP8044170.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7317..8492
                     /locus_tag="IVY85_001142"
     CDS             7317..8492
                     /locus_tag="IVY85_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8044171.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8496..9137
                     /locus_tag="IVY85_001143"
     CDS             8496..9137
                     /locus_tag="IVY85_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8044172.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9134..10051
                     /locus_tag="IVY85_001144"
     CDS             9134..10051
                     /locus_tag="IVY85_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8044173.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10048..10719
                     /locus_tag="IVY85_001145"
     CDS             10048..10719
                     /locus_tag="IVY85_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8044174.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11260..11748)
                     /locus_tag="IVY85_001146"
     CDS             complement(11260..11748)
                     /locus_tag="IVY85_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP8044175.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11949..12215)
                     /locus_tag="IVY85_001147"
     CDS             complement(11949..12215)
                     /locus_tag="IVY85_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8044176.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12215..12478)
                     /locus_tag="IVY85_001148"
     CDS             complement(12215..12478)
                     /locus_tag="IVY85_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8044177.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(12471..12743)
                     /locus_tag="IVY85_001149"
     CDS             complement(12471..12743)
                     /locus_tag="IVY85_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044178.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            12954..13139
                     /locus_tag="IVY85_001150"
     CDS             12954..13139
                     /locus_tag="IVY85_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8044179.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13281..15104)
                     /locus_tag="IVY85_001151"
     CDS             complement(13281..15104)
                     /locus_tag="IVY85_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP8044180.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15702..18407)
                     /locus_tag="IVY85_001152"
     CDS             complement(15702..18407)
                     /locus_tag="IVY85_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP8044181.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18713..19660)
                     /locus_tag="IVY85_001153"
     CDS             complement(18713..19660)
                     /locus_tag="IVY85_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP8044182.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19850..20119
                     /gene="rpsN"
                     /locus_tag="IVY85_001154"
     CDS             19850..20119
                     /gene="rpsN"
                     /locus_tag="IVY85_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8044183.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20295..20657)
                     /locus_tag="IVY85_001155"
     CDS             complement(20295..20657)
                     /locus_tag="IVY85_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8044184.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20668..21789)
                     /locus_tag="IVY85_001156"
     CDS             complement(20668..21789)
                     /locus_tag="IVY85_001156"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP8044185.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21805..22155)
                     /locus_tag="IVY85_001157"
     CDS             complement(21805..22155)
                     /locus_tag="IVY85_001157"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP8044186.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22285..23796)
                     /locus_tag="IVY85_001158"
     CDS             complement(22285..23796)
                     /locus_tag="IVY85_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8044187.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24118..25470)
                     /locus_tag="IVY85_001159"
     CDS             complement(24118..25470)
                     /locus_tag="IVY85_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP8044188.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25623..26699)
                     /locus_tag="IVY85_001160"
     CDS             complement(25623..26699)
                     /locus_tag="IVY85_001160"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP8044189.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26895..28640
                     /locus_tag="IVY85_001161"
     CDS             26895..28640
                     /locus_tag="IVY85_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP8044190.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28920..29357)
                     /locus_tag="IVY85_001162"
     CDS             complement(28920..29357)
                     /locus_tag="IVY85_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP8044191.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29341..30714)
                     /locus_tag="IVY85_001163"
     CDS             complement(29341..30714)
                     /locus_tag="IVY85_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044192.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31036..31524)
                     /locus_tag="IVY85_001164"
     CDS             complement(31036..31524)
                     /locus_tag="IVY85_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8044193.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31546..32148)
                     /locus_tag="IVY85_001165"
     CDS             complement(31546..32148)
                     /locus_tag="IVY85_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8044194.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9202 a         6884 c         5942 g        10163 t
ORIGIN      
        1 tttatatgac acacccgatt attcaatatt gagcaatata tcccatgctc ttatctcttt
       61 cttttttgaa taaaaaatga ggtaggacat ctttctgccc tacctcgtct ttaaataagc
      121 tttgatactt atttttcttc cttctcttct gtctcttcca gcgtttctgt tgtttcagtt
      181 tttacttcaa ctgtctcagc tggttcttct gttgtttcaa cagtagtcgt tgcatcattt
      241 gttacgacag tccctggttt gaccgttttg attgctgcac gatcgtactc aaggaagatc
      301 ccttcgcagt ctaaaacaac tgtccgtttg tcattgtcga tttctgaaac aactccatgt
      361 aatccgccga tcgttacgac tgaatcacct ggtttcatgc tatctaacaa gttttgacgt
      421 tcttgctgtt gtttcttttg cgaacgtgac ataaaaaacc acattgctaa tagcgcgata
      481 aacatcacaa gcattggtaa tgttcccatt tttttcacct cactaattct gtatctacaa
      541 aaaacacttt agcagaaaaa tgctgataac actagttttt tacttaattt ttaaaagatt
      601 ttcctttaga agctttttgc gttttcttta ttgaacccgt attcttcaaa aaaggcttct
      661 ctaaattcaa gcaaattatc atccatgatt gcttgacgta cttgtttcat tagattcaac
      721 aagaagtata aattgtggta agaagtcaaa cgaatcccaa atgtttcatc acatttgatc
      781 aaatggcgga tataggcacg tgtatagttt ttacatgtat aacaatcaca tttctcatca
      841 attggacgga aatcatgggc atatttcgca tttttcacta ctagacggcc ttgagaagtc
      901 atacatgtgc catttcgagc gattcgagta ggcaagacac agtcaaacat gtctactcca
      961 cggataacac catcgatcaa tgaatcagca gcaccgacac ccatcaagta acgtggtttg
     1021 ttttcgggga tcaacggtgt agtgaattct aatacacgat tcatttctga tttaggttcc
     1081 ccgacagata acccgccgat tgagtaacca gggaaatcca tcccgactag atctttcgcg
     1141 ctttgacgac gaagatcttc aaaccctgct ccttggatga ttccaaataa tccttgacgg
     1201 tctggattag catgagcttt caatcctcgt tctgcccatc gagacgtacg ttctactgat
     1261 tttttcacgt agtcgtaact ttcatcgaat ggtggacatt cgtcaaaact catcatgata
     1321 tcagagccca atttattttg gatgttgatt gctttttctg gtgataaaaa catacgcgaa
     1381 ccgttcaagt gatttttgaa atgcacgcct tcttcttcga tattccgcat atcacttaat
     1441 gaaaaaactt gaaagccgcc tgaatctgtt aaaatcggtt gatcccagtt cataaattta
     1501 tgcagtccgc cagcttcttc tactaagtct tcaccagggc gtagccataa atgatatgta
     1561 ttacttaaaa taacgccagc acccatttct ttcaactctt ctggggacat tgtttttact
     1621 gtagctaatg ttcctactgg catgaacatt ggtgtgggga aagttccgtg tggcgtgatc
     1681 aattccccta aacgcgcgcc agtatgtttt tctttcttga ttaaacgata gcgaatggca
     1741 ggttgcgtca ttcatttccc tactttctat ataaaatatc ttgacaactc ccaccatcat
     1801 aatcgtttca agtgcttttt gcaagttccc tcggcttcct tctactttat ttaatattct
     1861 tttttttatc ctttttttat gaataacaaa aaaaagaaaa acattatgac agaaatattt
     1921 tttgatacat ggtatattgg tgttattaaa gaaaaggagg gaaaaatcat gcaatcttct
     1981 gcagaattaa aacgccaagc aaaagagagt ctaaaaggac gttggggaca agctgtttta
     2041 ctaaatctaa tccctacatt gattggtatt gctactattc ttcttattat gattcctatt
     2101 atcttgtttg ccaccaccat atatgatgga tcagcgagcg tctcagaagc gacaacttca
     2161 ggaggcggta gttcaggagg cggtggcgga attatttcca gcatcattag cgctttgttc
     2221 atgagcggta tttcttggac ttatcttgat attttacgtg gacaaaaaga tcagatcgaa
     2281 ccattcaaag atgcatttcg tggattcaaa ggtttgttta tcggcggagt gatcagctta
     2341 gctgtgctga tctcgatctt tacaacatta tggggtttgc tattagttat tcctggaatc
     2401 attaaaagct attcttattc tcaaagctat tttatttact acgatatcgt ccaagaaaca
     2461 ggacaaacac ctaaagtact ggatacgatc actgcaagtc gaaaattgat ggatggttat
     2521 aaaggacaac ttttctggtt agatctttca tttatcggct ggcatatctt agcattagca
     2581 actgctggta ttggttatct atggctcagc ccatacattt ctgcaacaaa agcagcattt
     2641 tatgaacaat tgccaaaaga tatttaaaag agagtgtgac ttgtgaccga gggggtggca
     2701 ggagaacacc gtttatcgag aggcagtaaa agaaacattc agcccattag caaaaaggag
     2761 gtgtgacaac attcatgtca cacctccttt ttgtatagtt ccagtaaaaa gaaaatcaac
     2821 ttagaaacag ttaatcattc ctcttcttct gcttccggta atcgatggaa cttgatcagt
     2881 tcattcaccg cgattggtac gatagataaa atccctacca agaaccagtg attcaaactg
     2941 atgtgtacta ggccgaatag ttcttgcgta aatggcagca aagcaacagc cgttgtaata
     3001 gctaatgcca gtaacgccat ttctaccaaa gctttattgg tcgctaaatt gactttgaag
     3061 atcgattggc tgctgcgtac attgaacaca tgcagaatcg atgaataagc taagatcaag
     3121 aatgcaacag tttgaccgac atgctgactt gcgtctaccc atgggctgat agtatcgaca
     3181 taactaccta aataataacc aaacaaggtt acgatggtga agacccctgc atttatggca
     3241 attttttgca ataaacctcg agcaaagatc ccttcatttt tcggaatggg tggctgatcc
     3301 atgatgccgt gttccgctgg ctctcttcct aaagcaaatc ctggaagtcc atcagcaact
     3361 acattgacaa acagtagttg cacagctgta aatggtgctc cccatcctaa cagcatcgca
     3421 atcaaaacaa taaagatctc cgaaatatta cagcttagca aaaagttgat agctttacga
     3481 atattctgat aaaccgaacg tccttgactg acagcgtcta cgatcgtcgc aaagttgtca
     3541 tccgtcaaaa tcatatctgc ggcactttgc gccacatctg ttcctgtgat ccccatggca
     3601 cacccaacgt tacttgcttt caacgctggt gcatcattca caccatcccc agtcatcgct
     3661 acgactgcac ccgtacgttg ccaagatttg acgatccgga tcttgtcttc tggcgttacc
     3721 cgagcataga cagagagagc tttgacacgc gcatctaatt cttcgtctga taattgatgc
     3781 agttctgatc ctgaaagtgc ttctgacgga tcattcaaga tacctaattc tttcgctatg
     3841 gcactggcag tcaccacatg atctccagtg atcatgactg ttttgatgcc agcctttttc
     3901 gctcgcttga ttgctccttt actttctgga cgtggtggat cgatcattcc gatcagacca
     3961 atcaaacgta agtctttttc cagtgcttca gaagaaatat cttgcggttc ttcgtcataa
     4021 gtagcataac caacagcaat cactctcagt gcccgtttac caaagcgatc attaacgatc
     4081 gccgcttgtt ctacatcgcc tgaactaaag cgcggagcaa gaacatcgaa tgctcctttg
     4141 gtgattgaga tgtatttttc gcccatttgg tgaactgttg tcatcatttt tcgttcagaa
     4201 tcaaaaggaa gctcgtttac tcgtggatat ttttcttcta attctgcctt tgtatgataa
     4261 ttctcttcaa cagcccttac gatggctact tctgttggat tacctttgat aacaaggtcg
     4321 tcatcttctt gctcgaccgt cacatctgta catagtgcag ccattttcaa tatttccatc
     4381 gcgctgttcg tcattgaatc ttcgatattc gttacttcat cttctttgct ccatacccgg
     4441 cggacgcgca tttcattttg tgtcagcgtt cctgttttgt ctgagcagat aacattagcg
     4501 gttcctagcg tttcaactgc tggcaactgt cggatgatcg catgtttcct cgccatcttc
     4561 tgcacaccgt atgcaagtgt caacgtaaca ataacagtca aggtttccgg tacagctgca
     4621 acagccaagg aaatcgaagt catgaacatc tcaaggagtg gttctccttg cagttcacca
     4681 atcacaaata ctaaagccgc agccgctaaa gcgatcatac tgattcgttt gcctagctga
     4741 ttcaatcgga cctgtaaagg tgttttttgc atagtatttt cgttcaaaag atccgcgatc
     4801 ttacccattt ctgtagccat gccaatggca gtgacgatcg ctttcccacg acctgcagca
     4861 accgtacatc ctttaaatac catattaagt tggtcaccta atgactcatc ttcttcagca
     4921 ataaatgctg catctttttc aactgcttca ctttctccag tcaacgccga ttcttctaca
     4981 cgtaattgtg atgcttcgat caaacgggca tcggcagaaa tagcatcacc ggactctaaa
     5041 acaagaatat cacccactac cagttgctct gactcaacag tggtcacttt gccgtctcgt
     5101 atgacagttg ccgtttgttt attcatatct tgcaaggatt ccagtgcttt ttctgcgttt
     5161 ccttcttgga caatcgccaa taccgagttg atcacaacga tagcgatgat cagcagtcct
     5221 tcaaataaat ccccatgttc tgtagcaaat gcggtataaa aggaaatcac cgctgctaca
     5281 agaaggatga tcgttgtaaa atcggacaag cttcgaaaga attttttgat catcgattct
     5341 ttcggcgcct catcgaattt attcaatccc tgcttttttc gccgttcatc cacttcttgt
     5401 gaagacaacc cagtatctgg atctgcttct gttaccttct ttacttcttc tattgtctgc
     5461 ttgtattctt ccatcctatt cctcctttaa aatcttcgta caaaaaaagg tacttatttt
     5521 actataacat ccttttttta tttacttcaa ataaagggct tatcgattat ttgttaagaa
     5581 agcgtaaaat ttaaagtgct tatatttgca aacgctacca aaggatgcta ttatatagat
     5641 gtaaggaagg aggataccat caaatagaca gaagaatttc tcgatagaac agtgtaagca
     5701 aacaaagttg ttagaagtac aggtttcacc cagacatgcg tgtagagtac aaacctataa
     5761 atagtgaatg cgtatggatg tttggatcac gaagtatccg aaacaagaag taatgcgata
     5821 cgtaacaatt gaataacgta aacgtctgaa aattgaatag gaacaagaga tgtttcaaat
     5881 gttgaagatg taacgaaaac aaaatttttg tttaacatga agaaattcaa caccgtgctt
     5941 tgcatgtatg atggaaagtt acaatcattt ctgtttattt gaatccttcc gaggtacaag
     6001 aaagccgagc ttatcgagag ctcggctttt tctttgtcat aaaatctttg taaagctttt
     6061 ttgttattca aaactagcgg cttttcataa ccttatttca cgaacatcgc atctccaaaa
     6121 ctaaagaaac gatagcgctc ttcaatcgca tgctggtaag cagataaagt caattctctt
     6181 cctgcaaagg cactaactaa catgactaaa gtcgattttg gcaaatggaa attagttgag
     6241 aaagcttcta ccactttgaa ttcatagcca ggcgtaatga aaatatcagt ccatccgctg
     6301 tctgctttga tttctccatt aaatttcgtt ccaatcgttt ctaatgttcg aattgaagtt
     6361 gttccgacag ccacgatttt cccgccattt ttacgtactt ggttcaatcg ctcagccgct
     6421 tcttccgtca aacggtagaa ttcactatgc atatggtgtt cttcaatttt atctacgctg
     6481 acaggtcgga atgtacctaa ccctacatgt agtgtcagat agactaattc aacacccttt
     6541 gcttcgatct catcaagtaa ttctttcgta aaatgcaatc cagctgttgg cgctgcagca
     6601 gaaccatttt ctttcgcata gacagtttga tagcgatctg ggtcttccag acgttctttg
     6661 atatatggag gcaatggcat ttctcccaat gattcaagaa tctctagaaa aattccttca
     6721 tactcaaagt caatgatccg tccgccgtgt tccaattctt ctttcactac tgcttgcaat
     6781 ctgccatcac caaaagagat tcttgtccca actttggcac gtttcgcagg tttgatcaat
     6841 gtctcccacg tatctccttg tgtattcgtt agaagaagga cttctaaatg cgcaccagta
     6901 tctggttttt ctccatataa acgagcaggc agaactcgtg tattattcat aactagcgca
     6961 tcaccaggct gtaactcttc aatgatttca tggaaatgtt tatcttccat ctctccagtt
     7021 ttccgatcca aaacaagtaa acgagaatga tcgcggtctt ttaatggtgt ttgtgcgatt
     7081 aattcttccg gcaagtcaaa atcaaaatct tctgttgtta acatatcgtc actcactttc
     7141 tatcatgtgc ttcatcattc tatcatgttt ttcgttctgt tggcaaaatc agcacttttt
     7201 cacttctatt ttttgttttc ctaaataaaa aaactaccct atttgtattt tttcattgtt
     7261 ttaggcatac tagtaataga tagttcttca ttgaaaatag aaaaagaggt ggcaccatga
     7321 ttgaatttca aaatgtgtca aaaatttata aaggtggaaa agtcgctgta gaggacgtca
     7381 atctttcatt tgaaaagggt gagttcattt gttttatcgg tacgagcgga agcggaaaaa
     7441 cgacgacgat gcggatgatc aaccgaatga ctgatccaac acaagggaaa attttgattg
     7501 atggaaaatc catccaagaa attgatccag tggaattgcg acgtcagatc ggctatgtta
     7561 tccaaagtat tggattgatg cctcacatga cgatccgtga aaatatcact cttgtccaaa
     7621 aattgttgaa agtcagtcaa gaagaacgga ataaaacagc tgaaaaaatg attgatcttg
     7681 tggaattgcc ccgtgagatg ttagatcgct acccacatga attatctggt ggtcagcagc
     7741 agcgtattgg agtcgttcgt gcccttgcag cgaatcaaga tatcatcttg atggacgagc
     7801 cattcggtgc gcttgatcca atcactcgtg attccttgca agaccttgtc aaagatttac
     7861 aagagcgttt gggaaaaacg atcgtatttg taacccatga catggatgaa gcgattaaac
     7921 tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa
     7981 atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac
     8041 tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga
     8101 ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata
     8161 cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga
     8221 ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg
     8281 taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat
     8341 acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag
     8401 tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt
     8461 cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg
     8521 gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca
     8581 ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca
     8641 cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat
     8701 tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt
     8761 cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc
     8821 gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc
     8881 ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat
     8941 gggctacact tgcctcttat atcggcgctg gtggtttagg cgatttgatt tttagtggac
     9001 tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac
     9061 tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag
     9121 aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat
     9181 acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat
     9241 cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga
     9301 acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca
     9361 tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt
     9421 aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa
     9481 agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatagtt ttgaaaatac
     9541 gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga
     9601 tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa
     9661 aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc
     9721 tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg
     9781 ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca
     9841 attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc
     9901 tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga
     9961 attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt
    10021 acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa
    10081 caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac
    10141 ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc
    10201 atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag
    10261 acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat
    10321 gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc
    10381 gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct
    10441 agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata
    10501 agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt
    10561 ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta
    10621 gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg
    10681 gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag
    10741 cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata
    10801 atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca
    10861 ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt
    10921 tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc
    10981 ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac
    11041 atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt
    11101 cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa
    11161 tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc
    11221 catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca
    11281 actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc
    11341 gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt
    11401 gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt
    11461 gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt
    11521 attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca
    11581 ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg
    11641 ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac
    11701 tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc
    11761 ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg
    11821 tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga
    11881 acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc
    11941 ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca
    12001 tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt
    12061 gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga
    12121 atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc
    12181 gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat
    12241 cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg
    12301 tttgctcaag aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag
    12361 cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca tttacctctt
    12421 tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg
    12481 tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac
    12541 aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt
    12601 tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa
    12661 cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag
    12721 tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct
    12781 tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc
    12841 gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca
    12901 ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac
    12961 tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca
    13021 catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat
    13081 ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa
    13141 taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc
    13201 tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt
    13261 ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata
    13321 ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc
    13381 tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc
    13441 gctgcatttt tacttacgat atctacataa cggataattg gattttcaat cgagcgataa
    13501 gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt
    13561 tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg
    13621 atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca
    13681 agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct
    13741 gctacttttt tataatgatc atggatctta taaaaaatga acataacgat tggcataatg
    13801 atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat
    13861 gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt
    13921 ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag
    13981 tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct
    14041 agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg
    14101 tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg
    14161 gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata
    14221 ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg
    14281 atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc
    14341 atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca
    14401 ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc
    14461 aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca
    14521 gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat
    14581 gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc
    14641 atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc
    14701 gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg
    14761 atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg
    14821 acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat
    14881 gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca
    14941 gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca
    15001 tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca
    15061 gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta
    15121 cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta
    15181 cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa
    15241 tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt
    15301 tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt
    15361 tttgatatct taacttttgg aagagagatg taaggctgct ttctacccat cattcttact
    15421 actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc
    15481 tgcggtaata agcccaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt
    15541 attacttgat gcagagcagc ttttttacaa cctcttggta cacggtgttc aatcagctga
    15601 ccctcggtat tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc atttttttct
    15661 ctaatactca ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa aatatggctc
    15721 tcatgcgccg cttctttgaa tttcgtcatt ttttcttcga tgaacggata gattgccgtt
    15781 cctacgattc caaagacaac aaacaaaccg atcatgatcc agattgcttt cgtagcattt
    15841 ggccaataaa ttcctcctgc agattcccgt aacaagttta ccgcatgagt aaatggcaag
    15901 aacggattga tcatttggaa gaatttccct gagacttgga tcggatagtt cccgcctccg
    15961 ccagagatcg acagtactaa gatgataata gcgatccctt taccgacatt atcgaataat
    16021 gcagccaaga catagacgat catcatgaac gctaaggcga tcaagacagc aaatgacacg
    16081 ctgtagaccg gattttggac atctactcct agtaagaaaa tattccctaa tgtaacgatc
    16141 aagctttgcg caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt
    16201 ttcgtaaaat tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc aacacttgaa
    16261 agaagaacag cgccgaccca cagacatagt gcagtataaa acggggtgct agctgaaccg
    16321 ttatttgcga ttggataaag cgcattggtt tgtagctcaa caggttgtgt aaagaagtca
    16381 ctttcttttg ttgcgtctag tttcaataat ttgatgactt cgcctagatc gacttcttgt
    16441 tcgccttttc ggatcgcgtc tgctgctttt tggataccag ctttgatatt tggccagtca
    16501 ttgttgatca agtccgcagc agcgttcaca gctgtttcga cttctggcat tttttcattt
    16561 actgttgtca atgtaccggt gatttcattt tttagtgctg gccaatcatt ttggataaaa
    16621 tcagcggcca atcctaattt ttgctgaatg atcggtaatt cattgttgta taggtctgcc
    16681 ccttcattga tgccattgac gatggtatcc atatttccat tcagcattgt attggcatca
    16741 tggatttctt gtccaattgc aggcatttct ttttgatatt tttctaaaat tgccactgca
    16801 ttcgttacag ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg gtccgctttg
    16861 ctcagtgcgc cttgtgcagt cccaatcgta tcgatgactt ggtctagtag agaagtgaca
    16921 gtggaactga tttgacctgt attgatacca gcaatgatgc cattgatatt attaacaacg
    16981 gcatcaattt gatctagcaa ttcatcaata gccttcgtgt cttcattttg aacagctgtg
    17041 ttcaaagcag ataggcggtt ttgcagattg cttagcaagg gttttaattg atttagctga
    17101 tcaataattg gctgtaattt cgtatttcca gcacttgttt gcaatttagt caggaactcg
    17161 accagtttat cgattgcatt ttgttgttga gatattccgt caataaagcc gttcaataca
    17221 ttgctgatgt ttttcttttc atcatcagtc agcttaccat cttcaatagc tgttcttaca
    17281 gtatctatta acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct
    17341 acactgtttg tgatgcttgg tatcgcatct ttcagctgag aggcacctga ttttgtttgg
    17401 tttgctagat cacttgcctg attttctaaa tttttcacat ctggcaagat atcttgtact
    17461 tgttggatga tcgtcaagcc ttgttttgct tcatcgattc cttggttcat tgtctcttgg
    17521 atcgaagcaa aatcgccgtc gatctctgct agttgcttac cggcattttg gatctctggg
    17581 attttttctt gcaaatcaag gatgattttt gcttgatctt tcaactcagg cattttgtca
    17641 ttcaatgcaa cgactttttc acccatctca tctactttag ggatgtaatc cacgaattca
    17701 tttgctttat tcagtttttc cttgatttca ggtagttgac tttgtaattc gaggacttgt
    17761 tttgtgtagc cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat atctttaact
    17821 ttattgatac ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa tactttcaat
    17881 aaagtcgaac tggctgtttt gataaattca tttgtgattt gcgattggat cgaacttgct
    17941 cctttatcgg tgattttcgg tgcaatcgca ttgatttttt catttaccgt atattcgatt
    18001 tttggtttct tgatatcacc actagtaaaa ctcagcagat cttctgagaa atcctttggc
    18061 aagtaaatac ctgcgtaata ctttccggaa cggactccgt cttctaattg ttcttttgaa
    18121 tcaacgaatt gccatcctaa ttgtttattt ttatgaagcg attcgatgac ttgctcccca
    18181 atggccactt ctttaccttg aaactctgct ggcttgtctg cactgtaaac agcaattggc
    18241 agctctccag tatttccata tggatcccat aatgctttga tgttaaacca cgcatatagc
    18301 gaagggatga tcatgatcgc gatgatcaga agtgttgcaa tcgggttttt gaaaatccgt
    18361 ttccagtcaa gcaaatataa tcgaagcgta tttttgattg actgcatagt tctccccctt
    18421 cttctttttt cagctgaatt actttaccat gtatttcata cttttttcaa tcggcttttt
    18481 tcaaaaaagt cgaatttttg gacatatcac ggcaattgtc tcattgtaat ctcttacata
    18541 ttgagaaata attaaggatt acagccttta ctttatcatg aaataaaagg gaaagataat
    18601 tttatgccaa agtttttatc tattatacgt atacgcaaaa aagctaaaac attttttgtc
    18661 tttgccccta caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt tcttattttt
    18721 cgaatgacag gatgattttc ccagcaggag gatgtccttc aactagctga tgtgcttgga
    18781 tgacatgctt aagtgaagca gacagattca ttgaaataaa aacatgaaag gctccttttt
    18841 ggtaggcttt gattatgcca gcaaaggctt ctgcatctag atattccttt cttggaacaa
    18901 acgattcata aaagccttcc tttttttgcc gcaaatctag atctgggagg tcatttaatg
    18961 caacatacct gccgcctggt ttcatgatct ggatgccggt ttctcctttg atacttcctt
    19021 tcgtcgcatc gattacaagg tctgcctgat cagcaaattg cagacctgga tttgttttat
    19081 cataagcagc aaatgctgaa gctcctaatt tttttacctt ttcctcattt ttactggatg
    19141 cactggttaa gattcggatt cccttttcat ggagaagctg gatcagactt gacccgaccg
    19201 cacctgatgc tcctagtatc attacagtat ctgtcggctg gatttccgtc aaatggttga
    19261 ttagattata agccgtaatt cctggcgtga ccattccggc tgcttcttcc caactcattt
    19321 tatctggaat ttttgctact tttgctgatg gcaacacgac ttcttctccg taagtcccgc
    19381 cgactgcatg gacagctact cgatcaccta catgaacagt tgtgacatct gaaccaatct
    19441 ctgtcacaat accagcgcca tcattacctg gtacataagg gaatttcaat gttcgtatct
    19501 ctttcatttc tcctaaacgc aaggcaacat cataaggatt cacactgaat gcttttattt
    19561 ctacacgaac atgtccagaa ccgacttctc tgggatgagc atcgatttct tcaaaaacat
    19621 cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc tccctttttc
    19681 tttaagttta cgtttatgcc tttattttga caaaaaaaat gtcttttcgt tttggcgcat
    19741 gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt taaattattt atctaaaagc
    19801 gtaaagatta cgatttacgc taataaaaaa taattggagg aatttgttga tggctaaaaa
    19861 atcaaagatt gccaaagcaa aaaaacagca agaaacgatt gaacgctatg ctgctttacg
    19921 ccaggaatta aaagcgcaaa aagattacga aggcttgcgt aaacttcctt tagatgctcg
    19981 tccagaacga ttgaaaaatc gtgatttaat cgatggacgc cctcgcggat acatgcgtaa
    20041 attcgggatg tcccgtataa aattccgtga attagcacat aaaggtttga ttccaggagt
    20101 aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac
    20161 cactccttgg atcatcaaaa aagatacgtt cgcttcttct agcagaaatg ctggtactag
    20221 aagcagacgt atcttttttt acgcacagct ggtccttttt tatatgtaaa ggctgcatgg
    20281 ttacttcgct atatttattt agtaacagga gtcagtgctt cgatacccac actaattttc
    20341 aatgctcggt caacttctgc cataatgggt cgatccaaat ggcagacctt ttctttcaat
    20401 cttgatttat cgatcgttct gatctgctcc aacaggataa cggagtctct ttctattcct
    20461 gttccagtag ctttgatccc aatgtgagtc ggaagcttag gtttagccat ttttgccgtt
    20521 atggccgcaa caatgatcgt cgggctaaaa tggttaccta gattattttg aataaccaaa
    20581 actgggcgtg ttccgccttg ctccgatccc acgactggag aaagatcagc aaaataaata
    20641 tctcctcttt ttaccatagg atcctcctta ttcttgatat tctcttggta tccgctgtcc
    20701 taatccgcaa gccacttcat agtggatcgt acctaattga tctgctatat cctgcatagt
    20761 gatttctttg tctgcatttt tgccgataag cgtcactttt gttccgacag gaaattcttg
    20821 aggcaatcgg atcatcagtt gatccataca tacacgccca atcgtttcac agtagttccc
    20881 ttctaccaat agagaaaaac cctgcatctt tcgtggccag ccatctgcat aaccgatcgg
    20941 tatcgttccg atccactctg cttcaggagt gatatatgtt tctccatagc cgattccttc
    21001 acctgctggg agttttttga cttgaatcaa ttcagaaacc agctcaagtg ctggctgcaa
    21061 tggataaact tccggtagtg cgtgcccaga gggattaagt ccgtacattg ctactccata
    21121 acgaatcata ttgccgattg tttcatcatg ccataaagcc gttgcactat tgctgacatg
    21181 tacatatcga ggaagttcgg gaagtttttt cagcacttct ataaagcgtt ctttttgcag
    21241 attccaatat gtatcatctg cctgatctgc tgtagaaaaa tgggtaaata aaccttccca
    21301 ttctaataca cgactttctt tgatgaatgc agctgcctct tttacttctt caggtgtaca
    21361 aaatccgata cgacccatcc cagtgtctgc ttttagatga atcgaaagag gcgtctctag
    21421 cttacttttc tctaaaacag cagctgctgc ttgcaaccat tcccgtgtac ctgcagttac
    21481 tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa ggaaccatat tcaagataag
    21541 gatgggctga gtaaatccag cttctctcaa ctcgactcct tcatctaaat ttgaaacaca
    21601 aaagcccgaa gctccacctg caactgctgc ctcagctgtt tcaatagctc catgaccgta
    21661 tccgttcgct tttactactg caaacaattc ttttccttgc ggaagtcttg caacttcatt
    21721 tgctacattt tcagttatag cttttttatg tatcactgct ttcgttgggc ggtgccaagc
    21781 gacaaccatc gtttttcctt ctttctagtc ttccaaaata atttgtgcaa aagctgtttc
    21841 atttgtatga gtaatactga cccatacatt gccttcatga ggtgaacgcg tcacaacagg
    21901 ctgaccattt tcatttttta aaatctcgac atcttgaaag gtaacttttc cgatcccagt
    21961 tccccaagct ttagagaaag cctctttgca agcgtaacgc ccgcctaaaa attctacttg
    22021 gcgatgaaaa ggcaaggact ggaatagttt catttcatca gaggtcaaga ttcgtgcaat
    22081 gaattttggt ttttcttcga tcagccgagt gatcctagga agttcgactg catcgatccc
    22141 aattcctttt atcatatgct tttctctttt caataaattt agaacactct cattctacca
    22201 aattataacc attttgccac aaataagtcc acaaataaca aaaatgaaag tttctcttaa
    22261 aaaagagaaa ctttcatttt atgtttaatc gttatttgaa cggattacaa atccgcgttt
    22321 tttatcattg tgacgtttac cgccatcttt ttggctgtta ttatttttgc tataaccgcc
    22381 ttttgatttg tttccacgat aattaccgcc gtcacgacga ttacggttgt ttccgccacc
    22441 acgtttgcca tttttattgt atcctttttt ctgcattggc aatgggcgtt ctggtgtgat
    22501 cttcactggc actgcatcag ctggatcttt tgctgtcgtt tttagcaaaa gtgcaactaa
    22561 atcttgtgca gaatattctt ctaataattt atctgctgtt tgcaggtatt tatctagacc
    22621 attttctgca agttttgttt cgatttgttc aactgcagca cctaattgac ctttgaatgc
    22681 ttctttttcg gttggtggac gtaaagtcgt catccgtttt ttcgtcaagt tttcaatcac
    22741 atgcaagtag tccatttcat ttggtgtaac gaaagtcaca gacataccgc cttttccggc
    22801 acggcctgta cgtccgatac ggtgaacgta gctttctgga tcttgaggga tatcatagtt
    22861 gtacacatga gtgacacctg aaatatctaa tccacgtgca gcaacgtctg tcgctacaag
    22921 gatatctaaa tgaccgctct taaatgaacg caatacgctc attcgttttt gttgagacaa
    22981 atcgccatgg attccttctg cacggtaacc gcgtgcttct aatccgcgtg ccaattcgtc
    23041 tacacggcgt tttgtacgtc cgaagacgat tgttaattca ggtgtttgaa catcaaacaa
    23101 gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg acatagtatt gatcgatcaa
    23161 gtcagctgtc atttctttcg ctttgatctt cacatgatgt ggatttttca tgaatttaac
    23221 gccaatattc ttgatagctg gcggcatcgt tgctgagaaa agtaatgttt gtctttgatc
    23281 aggaacttgt gaaatgattt tttcgatatc ttccaagaag cccatattca acatttcatc
    23341 tgcttcatcc aaaactaatg tttgaacggt tccaagtttc aatgtgtggc ggttaatgtg
    23401 gtcaagcata cgtcctggtg tacccacaac gatatgtgga cgatctttca agccacggat
    23461 ctgacggcca atatctgctc cgccgtaaac ggcttgtaca cgtacttttt tgtcgcgccc
    23521 taaacgatat aattcttctt gtgtttggat agctaattca cgtgttggtg cgataactaa
    23581 accttgtaat tcgtgacgat ctggatcgat tttttctaac attggtaatc caaatgcagc
    23641 tgttttccct gtacctgtct gtgcttgtcc aataacgtct tttccagcta atgctagtgg
    23701 aatcgtttct gcttggatag gcgttgcttc ttcaaaacca gcgcgttcga ctgatttcaa
    23761 caattctggt gataattcta gttctttaaa tttcaaatga atcctcctat atggttgtta
    23821 actgaggatg tagctttttt gctaccccgt cgatttgttc ggtcaaagtg tattgtttct
    23881 ttttgacagt ggccacgcac gtttttatcc attcttagcc cctgtggcac caccgaaaaa
    23941 tcactttgcg aagtttaggc attagaaaag ccaaacgggt cgcgttcagc tttatcatta
    24001 ttatgacatc tggcttcaac aagttgtttc tatggcaaaa ccagtccaat ttctcttcat
    24061 ctttcggcaa taggttattc taacatagtt tttcgttttc tgcaagttta cagattttta
    24121 tttcatttgc tgaagttgtt ctaccacttc aaataagccc atgctgttgc tgccttttag
    24181 cataatacta tctgttgctt caagattttg tttgatagca tcaatcaatt tattcttttc
    24241 tttttcaaaa tagaacacag gtagttgggg gtatttttgg ttcaattctt cttttagtgc
    24301 atgcatttgt tccccataaa ggaaaatcat ggcgaatttc tcaggatcga tatgttcact
    24361 catttgcgca tgcatattta aagactcttt ccctaattcc agcatatctg ctaacacagc
    24421 gattctgcgt ccttttgttg gcagattttt aaaagtatct aataccagtc ccatggctgt
    24481 tggatttgca ttgtagacat cgctcaaaat agctgcacca tttcctgccg ttagccattc
    24541 ggttctgttt tgagtgatag aaacatgggc gagcccttgg cgtatctcag gtatggacag
    24601 tccaaagaag cgcccgatcg tatacgcaat caaagcattc gttacattat aacttccagg
    24661 cagcgggatc gtaaatgttt ctccttctac tacaaaatcg gtttgtgctt ttccttttgc
    24721 tgtcacttcc gcatgaacat cgccatttcc aataccgaat gttgtgactg tttgtactaa
    24781 tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc acaacgagta atccgtctga
    24841 tacaagtccg tcagtgattt ccattttggc tttagcgatt ccttctcgcg acccaagatt
    24901 ttctatgtgt gcttcaccga tcattgtgat tgctgcagct tttggttgag ccataagtga
    24961 aagttccgtg atttcattgg cgtgatccat gcccatttcc aaaacgatct tttctgtatc
    25021 ttggggcatg tgcaaaatcg tgtaaggaag accaatatta ttgttgtaat ttccttgcgt
    25081 cttatatgtt ttgtattttt cagacaagac agacgcggtt agatctttcg tggtggtttt
    25141 accattgctt cctgtaattg caacgacttc tgcgcctact ttttccaagt aataggttgc
    25201 caattgctgc atggctttca aaggatctgc tacaggaata actggaaaat tttttggagc
    25261 ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg tttgtgatcg cttgctggat
    25321 aaattcatgt ccatctcgcg taccagcaag aggaacgaat aaacttcctt cggttatctt
    25381 ccggctgtca aattcgacat tggtaatctt tgtcagtgca gagcattcgc tgcccacggc
    25441 ttgtgcaatt tcttgtattg ttagattcat tgagatctct cctaaaaaag gttgtgaaaa
    25501 aagcgtttgg cctaaacggc gggttcgcat gactctcact tccaagtaaa tttctctatt
    25561 cgtatttaga agctagaatg aacgcggtta cgcaatcact ccagcctctt ctttatttct
    25621 ctttattcat ttttttcaaa aaaagattga cgctgatggt atcgattcat tcctaactgg
    25681 atcagttctt caatcaaatc accgtatttc aagcccatat tttcccataa gagtgggtac
    25741 atactgaact ccgtaaatcc tggcatagag tttaattcat tcaggaataa ttcattttta
    25801 tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac ctaacatcgt gtaagctaac
    25861 ttcgcgtact cttgcgcttt ttgataaact tcttctggca cttcggctgg aatctgcatt
    25921 tcgattttat tattgatata ttttgcttca taatcataga atgctacgtc ttttacgact
    25981 tcaccaggca aagtcgtccg aacgtcttca tttcctaata cagcaacttc gatttcgcgc
    26041 gcttcaattc cttgttcaac gattgctcga gaatcatact gataggctgt tgctaaagca
    26101 ttttgcagct cttctcggtt ttctgctttt gtaatgccga cactagaacc catattcgcc
    26161 ggtttgacaa acatcggata aagcaaagaa ccttcacatt gatcaaatac ttttttagga
    26221 ttttctttcc attgattctt aagtactggt acataaggaa cttgcggcac accagcagct
    26281 tgtaaaatat acttggtcat gattttatcc atggcacatg cactggtcaa tacgcctgcg
    26341 ccgacataag gcatattcaa tgtctctaag aagccttgga tcgttccatc ttccccgttt
    26401 ggcccatgta aaactggaaa aacgatggct ccttcttctt tgatttcgcc cggattgatc
    26461 acttttcctg taaagccttc ctctgtctgt ccacttgggt cccatgaaag atgcaagaca
    26521 tctttgctgg caggtttttc tgttagtaat ggacctttga cccattgtcc ttcttttgta
    26581 ataaatacga gttgaacttg gtaataatta taataaatgg catttaaaac tgaaaatgcg
    26641 gaaagaatgg acacttcatg ctctgcgctg cgtccgccat atagtaaagt aatcttcaaa
    26701 agattgtcct cctgtattct aaatcgttca gtgatttact ctcatatttt agcacaaaac
    26761 aaaaataaac gaataactta tttataaaaa aacgagccag cttctaaaag aacgagcgtc
    26821 atttattttt tgtcagctag attatttgtc ccgtttttta taaaaagtta tgataaagaa
    26881 aaggagggat gatgatggcg caaacaaatc cacgcagccg cttggcagaa atcattcgag
    26941 tgttcatcaa ttacaatgta gtgccaaact ttgtacagca aaaaaatccc gaacaggtaa
    27001 aaaaagcctt tgaagaacta ggccccacct tcatcaagat aggacagatg ctttctgttc
    27061 gagaagattt gctttcttct gccttcactc aaacattcaa aacattacaa gatagtgtac
    27121 ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac agaactttct ctttcattat
    27181 cagatatatt cgatgatttt tcaaaatcgc cttttgcctc tgcttctatg gggcaggctc
    27241 atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa gatccaacac cctaacatcg
    27301 ccgaagagat ccgacttgat cttcaactat ttgaacgagc gatccctttg atcaaataca
    27361 ttcctgaaac tagcgtagtt gatctaaaag gggtactcca agaagtaaaa cgttccttga
    27421 tcaatgaaat ggacttctta aaagaaagtc aaaatggcga acaattctat caaaagaaca
    27481 atggctggaa agagatccgc agccccaaga tttatgacgc tttttgttcg aaaaaagtga
    27541 tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt aatgaacgcc gaaaacaaaa
    27601 ctgaaacttt tatcactgga attcaaaaca agcagctgaa acaagaagtt gcaaagcttt
    27661 tagttgagaa ctttatgaaa caggtgtttg atgatggttt tttccatgct gatcctcatc
    27721 cagggaatct gctattccat gtactaacaa aagaagaaca gacacaagct agccgaaaaa
    27781 ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt tcgagcctcc acttctgcag
    27841 aagaccctgt tgcgccatat accatcaact acatcgattt tggtatgatg gggcatcttt
    27901 ctgcaggact taggcaaaaa ctcacacaag ccgttttagc gctgtatacg aaagatgctt
    27961 atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga aggctcgttt gatgaaagtc
    28021 ggttccacca agaactgaca agttttcttg aacagtatta tgattctcct attgatgaaa
    28081 tcaacttgca agaagtattc acacatgtcg tgacgatttg tcatcaaaat aatctgcagt
    28141 tcgatcgtga tatcacgctt ttgctgaaag catttgggac attagaaggg gtgatccgcg
    28201 tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc ttttgctcaa cattattttc
    28261 tcactcatct ggatgtagaa gatacattga aacaaagcgg attagactta ttggaaggga
    28321 tgaaggctgc gcctaagatc cctcaacagc tccaccatct attagaaatg tggacttcgg
    28381 gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa attattgagc aggatcgaaa
    28441 gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc attgatcgta gggtcctccc
    28501 tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt ttccttatta gggatattta
    28561 cgtatgctat tgccgcattc gtcattattt ttttggcaat cgatgcattg atccaaatgt
    28621 ataagaagag gaaaaagtag aaaaaggttg tgacagaaag aatcagcttc aagaaataag
    28681 aaggggtttt acataaattg tttctcaaat ttttgtgaaa attcggctta tttttcgaag
    28741 aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa gctgttctgc acccagtaat
    28801 aagaacaatc aaaaagggaa catggaacac atttatcctg tttccctgtt ccctttttga
    28861 tgtattcgag cataaataaa ataccttcgt tacctactat cttctgtact tggtaaacct
    28921 tagtcaattg ccttttttaa cgcttgacga aaagacatat gatctttctc cgtaaagggt
    28981 tttggaccct ttgtccttat gccagctttt cttaactttc cactatgata acgttgttct
    29041 agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag agtgatggaa cactcgcaca
    29101 cccttcggca atacaagtga agctaaacca taatcttgcg tgatcaaaaa gtcattcttc
    29161 ttaaccatac cgacgatttt ataatccgca ctatcagctc cgcggtctac gtagacgatt
    29221 tggacatgat tagcgtattc tttggtcgta taatggtcca cactggtcac gataaccacg
    29281 tcaagaccat aggattctcc taattttatg acatcttctt ttactggaga accatctcca
    29341 tcaatgaata gcctcattct ttttcgcctt cttccataaa tgataaacag ttactaatcc
    29401 gctcagtaaa agaccaacat tgatccattt tgccaagata agcagttgta cactttctat
    29461 cggcactgat tcatggttca gcgtgatcaa taatagatgg attggctctc ccatttcttt
    29521 tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg atcacactcc ctttattttg
    29581 agtagtatac ttgacaacga tccggcgatc cattggaata gctgtatcag tttgataata
    29641 ttccttattt agccagctgc ctgttactac gtgccagaaa ctaagcagaa agacacctat
    29701 cgcaaaacca tttggaatca agaggaccaa taaagaagaa ctcagtccaa gcaattgcat
    29761 agccaatgga gaccatgtta aacgtttcaa aatccatggt ccgactattt ttgctgcaat
    29821 caagcctgct gcataaggca aatacataaa aatcgaaagt ttttctaatc cgtacagtat
    29881 gccaacataa aaactgctaa ataaaaaaag gaaattccct agcattccat ttagaaaagt
    29941 aaacagattt agccagcttg gcaacttcca ttccttcttc tggtgtccta aaacaaacac
    30001 gaacaagatg aacagcagca agaaaccata aatagcaaga tcaaaaagcc tttcatcgaa
    30061 taacaatcgg gcatttctca aaagaaacaa taagccaaag aatatcaaaa ataaaacaag
    30121 ctctcgcgta gctaccagat ttcttttgat gtctttgaaa tccagttcgt aacgcggata
    30181 atgactaacc gtatggtagg ccatgacgta aaaaagtgta taaatagtaa agcttgctgc
    30241 aatctgagtc ggtgcaggca actcaatcgc ttgatacagg agaaatagcc agatagctgc
    30301 aaataagtac tttttcccat ccatattgat aaaaccttgc gtcttttcgt gaaaattgac
    30361 cgtggtatta gcaggtacaa gccatgctgc actcagccct agcaaaacac ttgaaaaata
    30421 aaataaagga aaataaaata cgcctaatat tccaagcaat gaaccagcac caccaatcag
    30481 cagagcgatc atcagcaacg tgtaactatc tagaccaaat cgtagactgt tgactaaaaa
    30541 tagtccagtc atccgaaagg cataaaacag cgtaaatggc aaaatcgtca gccaattatg
    30601 cgagtctgtt agtcccaaaa ataaaagata aggaataaaa ataaccgtgt tacttaagat
    30661 aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg agtttttcga acatgacaat
    30721 ggctccttaa acagtcttat aactaatctg ctcttcgatt ttagcattaa aggggaaata
    30781 aaccaatttt acagaatata ttcttgtaaa aaaaaataca gaaagaagca taagcttctt
    30841 tctgcatttt tacggtactt gattactcaa gcagacaagc ccttctccac aaaagtcttg
    30901 cccgacaccc ctgcatccgg caggtgataa agatagaaat caattatcct gtgcctccaa
    30961 aatcaattag gagctctgta aaccagagtc cttctcgacc cgtcgcataa atttacttta
    31021 acacagttcc tatgtttatg cgagagcttt tttcatttta taatcgacca tttgaaatcc
    31081 ttgaccgata tctgaaataa gttcttctgt aatctcaaat ccttcatgct tataaacctc
    31141 gattgcttga tgattcccct gattcactcg caaaaagatc tctctttcct ttgtttgcgc
    31201 agcaagttct tcataccagc gaaaaactga agaagtcaat ccttgaccac gtactttatt
    31261 tagcaaatat aatttactta aataaatttt tccatttatc tcttcataag cagtgtaacc
    31321 gacagtcttt tcttcgcttt ttaataaaaa gtaggacaca ccttctgtca gttctttttg
    31381 aatctgtttt tttgactgat aagttttcag catgtaatcc acttgttttt gaccaatgat
    31441 cggcacaaaa acttccggcc atacttcttg tatgactgcc atcagctcat ccagttcatt
    31501 ttctgttact tttatttttt ccatacaccc acgtcctttt ttacattata aatgaccttg
    31561 cgcttgatgg aataaaaaga gcatgatcat gctatacaca cccaccgacc acggtttcaa
    31621 gaaaatcaca atctgcataa attccctaaa agataatttc gtcaaaccag ctactagaca
    31681 gatcaagtcg tctggcgcaa agggaagcag caaggtaaca actagtactt ttttaatatc
    31741 tttcgttttt cttgtcagca attcatcaaa tttctgatac ttttttggcg ataaaataag
    31801 ctggacaaag gctacgccat agtaacggac aagcaaaaat ccgattgctt ccccaatcac
    31861 taggccaata caactatata aaagcccaaa tccattgcca aacatcagca tgcccgctac
    31921 tgaggagatc cctcccggca aaataggaat gatcacttgg atcacttgca acagaacaaa
    31981 tatcatcact gcatacttgc caaattgctg gataaacgct tgcaaagacg tgctcgatcg
    32041 aaaaatccca tgatgataag cataaagaat caacacaaag aaaaaaaaca gaccaataat
    32101 gggcatgatc tgaagacatt tttgtaaaac tgatcctttt ttagacatct atattcccca
    32161 catctttttt catttattaa aactgcctcc t
//
LOCUS       DACCYH010000027        30637 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.027, whole genome shotgun sequence.
ACCESSION   DACCYH010000027 DACCYH010000000
VERSION     DACCYH010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.027"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            147..518
                     /locus_tag="IVY85_001166"
     CDS             147..518
                     /locus_tag="IVY85_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP8044195.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(792..1484)
                     /locus_tag="IVY85_001167"
     CDS             complement(792..1484)
                     /locus_tag="IVY85_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8044196.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1487..2272)
                     /locus_tag="IVY85_001168"
     CDS             complement(1487..2272)
                     /locus_tag="IVY85_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8044197.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2509..3909
                     /gene="lacG"
                     /locus_tag="IVY85_001169"
     CDS             2509..3909
                     /gene="lacG"
                     /locus_tag="IVY85_001169"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP8044198.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            3934..4251
                     /locus_tag="IVY85_001170"
     CDS             3934..4251
                     /locus_tag="IVY85_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8044199.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4264..5937
                     /locus_tag="IVY85_001171"
     CDS             4264..5937
                     /locus_tag="IVY85_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8044200.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            5951..7582
                     /locus_tag="IVY85_001172"
     CDS             5951..7582
                     /locus_tag="IVY85_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP8044201.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8073..9638)
                     /gene="guaA"
                     /locus_tag="IVY85_001173"
     CDS             complement(8073..9638)
                     /gene="guaA"
                     /locus_tag="IVY85_001173"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP8044202.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9697..10620)
                     /locus_tag="IVY85_001174"
     CDS             complement(9697..10620)
                     /locus_tag="IVY85_001174"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP8044203.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10816..11421
                     /locus_tag="IVY85_001175"
     CDS             10816..11421
                     /locus_tag="IVY85_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8044204.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11729..12745
                     /locus_tag="IVY85_001176"
     CDS             11729..12745
                     /locus_tag="IVY85_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8044205.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12886..14187
                     /locus_tag="IVY85_001177"
     CDS             12886..14187
                     /locus_tag="IVY85_001177"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP8044206.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14233..14895
                     /gene="deoC"
                     /locus_tag="IVY85_001178"
     CDS             14233..14895
                     /gene="deoC"
                     /locus_tag="IVY85_001178"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP8044207.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            14916..15314
                     /locus_tag="IVY85_001179"
     CDS             14916..15314
                     /locus_tag="IVY85_001179"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP8044208.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15380..16462
                     /locus_tag="IVY85_001180"
     CDS             15380..16462
                     /locus_tag="IVY85_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8044209.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16674..18236
                     /locus_tag="IVY85_001181"
     CDS             16674..18236
                     /locus_tag="IVY85_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8044210.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18229..19362
                     /locus_tag="IVY85_001182"
     CDS             18229..19362
                     /locus_tag="IVY85_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8044211.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19362..20318
                     /locus_tag="IVY85_001183"
     CDS             19362..20318
                     /locus_tag="IVY85_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8044212.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20417..21583
                     /gene="deoB"
                     /locus_tag="IVY85_001184"
     CDS             20417..21583
                     /gene="deoB"
                     /locus_tag="IVY85_001184"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP8044213.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21607..22425
                     /locus_tag="IVY85_001185"
     CDS             21607..22425
                     /locus_tag="IVY85_001185"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8044214.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22442..23149
                     /gene="deoD"
                     /locus_tag="IVY85_001186"
     CDS             22442..23149
                     /gene="deoD"
                     /locus_tag="IVY85_001186"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8044215.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23447..24133)
                     /gene="gpmA"
                     /locus_tag="IVY85_001187"
     CDS             complement(23447..24133)
                     /gene="gpmA"
                     /locus_tag="IVY85_001187"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8044216.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24305..24886)
                     /locus_tag="IVY85_001188"
     CDS             complement(24305..24886)
                     /locus_tag="IVY85_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8044217.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25019..25702)
                     /gene="rpiA"
                     /locus_tag="IVY85_001189"
     CDS             complement(25019..25702)
                     /gene="rpiA"
                     /locus_tag="IVY85_001189"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP8044218.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26021..26434
                     /gene="rpsL"
                     /locus_tag="IVY85_001190"
     CDS             26021..26434
                     /gene="rpsL"
                     /locus_tag="IVY85_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP8044219.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26518..26988
                     /gene="rpsG"
                     /locus_tag="IVY85_001191"
     CDS             26518..26988
                     /gene="rpsG"
                     /locus_tag="IVY85_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP8044220.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27082..29169
                     /gene="fusA"
                     /locus_tag="IVY85_001192"
     CDS             27082..29169
                     /gene="fusA"
                     /locus_tag="IVY85_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP8044221.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29341..30528
                     /gene="tuf"
                     /locus_tag="IVY85_001193"
     CDS             29341..30528
                     /gene="tuf"
                     /locus_tag="IVY85_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8044222.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
BASE COUNT         9387 a         5644 c         6713 g         8893 t
ORIGIN      
        1 atagaatcaa cactcagaga acgggttggt ttttaataga tttaataagt actgataatg
       61 aaatttctcc tggttgggaa aacgaagttg gttaccctgt agaagaattt tttcaagatt
      121 actttgcgga gtatgccgat aggataatga gagattacgt aacagataaa actcaagaag
      181 tcatcaatca atcccagtca ttaaatgaat tatctgataa ggcgaaagaa gaagcagttg
      241 ccattatttc ttcagggatt agtgactata ttgatatgag tggtgaaatc aattatggcg
      301 ataaagacaa aattgtacgc atgataaaag cttttatcaa aacttttgat gtgaaggcag
      361 atcttaagtt taatgatact tacctagtag gaaaattaat taatatcctt gggacagaga
      421 aagatacgga aacgtttatt tcctctttgt ctatgttgtt tacagataag catttttcaa
      481 ctcagcttgg tggagaggaa ctagtggaaa tcttttaacc attacgccca gcattaataa
      541 aagtatatca aaaaagtact cctgatgatt tttaagattg gtttgagaaa taatgtgaaa
      601 acactatttt taatgttaat tatagataga gatgaacgcg tcatatggtt aaaagaatga
      661 aaaagtcaaa gatacactgc tatatttcac taaggatata gcagtgtatc tttcatgtcg
      721 tttcactggc agttttttat aaaaggaggt gtgcatttca gttgcttttc tttggtagtt
      781 agttgaatcg tttaagaatt aacggatgtg gcagacaggt gctctgtcca aaaaccagta
      841 aaatctttga tggttccatc tttatttagc tgtgcctgtt caaatactgt tttgttttta
      901 ccgaatggat caattgcaat aattgttccg atccccgcac gtttggcagc aagtaatcct
      961 gagtaagcat cttcgataac aagacaatca gccggagcta agctcagttt gtctgccgct
     1021 ttaagaaaaa tatccggttg cggtttccca gggaaagagc cgtcgtcata gacaatcttg
     1081 tcccagtcaa accagcgggc taaatcgaaa atatcaaaat aaaaagcaac attctctttg
     1141 acagtagccg tggcaatcgt taatggaatc tgttggtcag tcagctggtc aagagtcgct
     1201 tcaagccctt ttgtcaactg aagctcgttt tcattttgca agcaaagcat acgataatag
     1261 ctttcttttt cttctgacaa tacggcgatc tcagaggcgg tcaaatgctc tgaaatgaaa
     1321 tgtttcaaaa tctccgtatt cgtccgacca tgaatatttt ttaaaatatc ctcgtctgat
     1381 aaaccatctt ttgtatgatc atgtatcata tgcagccaag cagcttcatg cagatgagag
     1441 tccaaaaaca ttgttccgtt gaaatcaaat ataatccctt tcatagttac gctcctatag
     1501 tggtattttc ggttgaatca gcaatttgca gatcgacata ttgtgcatag tgctcttgaa
     1561 cctcccgcga gatctggcga tccgtaatga gcagatcgaa atcatgcaaa ttataaaaca
     1621 cataaaaatc atctcgattg aattttttat tatcaacgag tagatatttc ttacgggaat
     1681 tatttagagc gatctgctgt gcttctcctt cttcggtact gtaagtagag atcgcttcgt
     1741 tgtgaacgcc atttgaactg ataaatgctt ttgtaaactt tagtcttctt aaattgtcgt
     1801 tggtgatcgg tccgacaaat gcgccggtat ttttccggaa atcaccgcca atcagtagaa
     1861 tttctggtcc atctgcttga gcaagaatat caaaaactgg ataattattt gtaatgatcc
     1921 gaattttctt gttcagtaaa tgcgtcgcca atagttcgat tgttgtacct ggtcctaaga
     1981 aaatcgtttc tccctcgtga atcaaggagg ctgctgtggc agcaatttct tttttttctt
     2041 gaatctgcag agtggctttt tcagtgtgag aaagttcttg atctaaagtg taggaaagac
     2101 tttgagcccc tccatggaca cgcagcagct tgcccgattt ttctaattca tctaaatctc
     2161 tgcgaacggt catatcagaa acatctaatt gattgatgat ttcattaacg gtaaggattc
     2221 cttttttatt gaccatttct acgatagtaa gcaatctttc ttttttcaac attgtcttca
     2281 cctctaattt aaggataatc tatcatataa acggacaaca gtcaatctaa ttcgaacaaa
     2341 agtaaacatt tagggtggtt tttatgtttg ttacaataga ttttattgct gttttctggt
     2401 ttttatgatt atttgtgttt gtttttgttg acaaatgttt tggatgcgtt tacaatgcaa
     2461 tcagttagaa agagcgaaaa ctgattatag atttgaggag agatgtacat gcttaaatta
     2521 ccagaagatt ttatttttgg cggagccaca gcagcctatc aggttgaagg agccactaag
     2581 gaaggtggca aaggcgctgt tgcttgggat gactttttag aggaacaagg gcgattcagt
     2641 cccgatccgg caagtgattt ttatcatcaa tatgcaaagg acattgaatt gtgtgaacgt
     2701 tttggtgtaa atggtctaag attatccatt gcttggagcc gcatttttcc tgatggagct
     2761 gggaaaccaa atccagaagg aatcgctttt tatcatcgtg tgttcgaaga atgtaagaaa
     2821 agaaacgtaa cgccgtttgt gacgttgcat cattttgata cccctaaacg tttgtttgac
     2881 catggagatt ttttgaatcg agaaacaata gaagcatttg tttcctatgc cattttttgt
     2941 tttcatgaat tcaaagaagt caaggtgtgg agtaccttta atgaaatcta tccagttgca
     3001 accaatcaat atttgttagg tgttttccca ccaggaatca aatatgattt tacaaagatc
     3061 gtcgcctgtc ttcataatat gatggttgct catgcccgtg tagtgaacta tttcaaagaa
     3121 aacgaacttc cgggtgaaat tggtgtagtt cactcattag agacgaaata tgcggcaaca
     3181 gacgcgccgg aagataagca tgctgccttt ttagacgatg cattgtcgat ccgtttctta
     3241 ttagatgcta cctatttagg ctattattca actgaaacct tgactgctct tgatgagata
     3301 tgtgaagcaa atcaggccag ctatcatttt cctgaagaag atttcgttga attgaaaaaa
     3361 gcatccacgc gtaatgacta cttagggatc aatcactacc aatgccattt tgtcaaagct
     3421 tacgatggag aaaatgcaat ccatcataac ggtacgggtg aaaaagggac ctctgtttat
     3481 aaagtgaagg gaatcggaga acgtatttat aaagaaggaa ttcctcgaac tgattgggac
     3541 tggctgattt atccagaagg tctatatgac ttgttgctgc gaattaaaag cgattaccca
     3601 cattacaaca aaatctatat cactgagaac gggatggggt ataaagatca gtttgaagat
     3661 ggcattatta tggatcagcc gagaatcgac tatttgagag tctatttgga atccttaagc
     3721 aaggcgataa cagccggggt aaatgtgaag gggtattttt tgtggtcgct tatggatctt
     3781 ttctcatgga caaatgggta taacaaaagg tatggcttgt tttatgttga ttttgaaacc
     3841 cagaagcgtt acccaaaaga atcagcttac tggtacaagt tggtcagtga aaccaaaacg
     3901 attatttaat aagtgataat ggaggaagaa aagatgaata aagaggaatt acaaatgctt
     3961 ggttttgaga tcgttgcata ttcaggcgac gctcgcagta cattattgaa attattgaaa
     4021 gaagtaagac aaggaaactt tgaacatgtt gattcggcgt tgaaagaagc cgacgaaaat
     4081 ttaacccttg cacataattc gcaaacaaaa attttagcgg aagaagcatc tgggaaagag
     4141 atggagatgg gattcatttt tatccatggg caggatcatt taatgaccac tttattgctg
     4201 agagatctga ttcaagactt tattgtactg tacagacaga atcaaggata ggagaagaac
     4261 gctatggatg gaatcattaa acaaatcgaa aaagggaaac cattttttga aaaaatctca
     4321 cgtaatagtt atttaggagc cgttcgtgac ggatttttaa cagcgatgcc agctatttta
     4381 ttttcaagca ttttcatttt gattgctgcg gttcctgaga tttttggtta ccaatggcct
     4441 gaatctgtct ccacatggct atggaaagtc tataattatt cgatgggtgt tgtagggtta
     4501 ctcgtttcag caaccactgc tcgctgcttg gctgattcga tgaatcgtcg cttaccgaaa
     4561 aacaaagtaa tcaacaccac ctctgtgatg ctggcctctg tggtaagttt cctgttgtta
     4621 agcgtctctc cgattgaagg tggattttca acggaatata tggggacaaa gggactactg
     4681 gcttcctttg tcgctgcatt tttaacagtt aatttatata agttttgtgt gctgaaagat
     4741 attaccatta aaatgcctaa agaagttcct ggaacgattt cgcaaacctt ccgagatatt
     4801 tttcctttct ctttttcagt atttgcggcc gttttaattg atttaggcat tcgacaaatc
     4861 ttccaaatct catttgcaga agcaatcatt acggtgctgc agccgctatt tacagcagct
     4921 gacgggtact taggaatcgc tattatctgg ggagcgatgg cgctgttttg gtttgtcggt
     4981 gttcatggac cgtcgatcgt ggagccggca attgcggcaa ttatctatgc caatgtggaa
     5041 accaatctgc aattgttcaa ttccggtgaa caagcttcta aagtactgac ggtgggttta
     5101 gggaattttg taggaactat gggaggaact ggggcaacat tggttgttcc ttttatcttc
     5161 ctactctttg ctaagtctaa gcagttgaag gcagttggga aagcttcctt tattccagta
     5221 agtttcgctg tcaacgaacc attgctattt gcggcaccca tgattttgaa cccttatttt
     5281 tttattccat ttttgttaac acctgttgta aacgtttgcc tgttcaagtt ttttgtcgat
     5341 gtattgggca tgaacagctt tatgtacgtt ttgccttggg caactccagc accgatcggt
     5401 ttggttctag gaacgggcat gggtatctta gcttttgttc tagcagcggt tctaatcgtc
     5461 gttgacttcc tgatctatct accattttgc aaagcatacg atgctgtctt agtagagaaa
     5521 gaagcacaaa aagcagaggt gatcgcaacc gaggaaggtg cttctgccga tgtacgtgag
     5581 gaagtacctg tggcagcgag tcaagttatc agcgatgcaa ataaagaaat caatgtgttg
     5641 gtactttgtg ctggcgcagg gacaagtggg atgttagcta atgctttaaa aaaaggtgca
     5701 tctgaatcga aagcaccgat cagtgcaatg gcgggtgctt atggttctca ttacgaaatg
     5761 ctgcaggatt ttgatatggt ggtcttagca ccgcaagtca attcttacta tgatgatatc
     5821 aaagaagaca ccgatcgctt aggaatcaag ctggttgcga caaaaggagc agaatatatc
     5881 ggattaacga gagatcctga aaaagctgtt cagtttattc tgaaccaatt tcaataaagg
     5941 aggagccata atgaaaatgg gaatttggtt gcgtaaagct gttttatttg cagctgtgtt
     6001 aggggctttg ggggctgttt caaaaactgt tgaagcatct gccagcgaat ttattcgcgg
     6061 agcagatatt tcgatcctgt cagatatgga aaaatctggt gcaaaatatt atgaagacga
     6121 tatttcaaaa gatgccttag agattttaaa aaataatggt gtcaactatg ttcgactgcg
     6181 tttgtggcag gatccttata atagtaatgg agaaagttat ggagcaggga caaatgatct
     6241 ggaaacgacg attgaccttg caaaacgagc aaaaaatctg ggacttaagg tgctgctgga
     6301 ttttcactac agtgattttt gggttgatcc tgggaaacag aatcttccta aagcatggca
     6361 aggtctgacc tttgaagaaa tgaatacggc attgtacgac tatacaaaga acgttctgtc
     6421 ggaaatgaag cagctggacg tttatcctga tatggttcag atcggcaatg aactgaatag
     6481 cggaatgctc tggccttatg gcaagtcttg gggagaaggc gggggagaat ttgatcgatt
     6541 ggctgctttt ttgaagtctg gtatccaagc tgttcgagac actcaaccta aaacaactcc
     6601 agtaatgctc cacttagctg atggtggcga tacaggagca tttacttggt ggtttgatga
     6661 aatcacgagt cgaggtgtgt catttgattt aattggagtg tcgtattatc cgtattggca
     6721 cggttcaatg aatgaacttc aaaccaatat gaatgcgatc agccaacggt atggtaaaga
     6781 agtgatcgta gttgaaacag cttatgggca tacaacagcg aatgccgata ctatgccaaa
     6841 tgcttttggt gaagccgaag cagctgcggg gggatatgaa ccaacaccga caggacaagc
     6901 agagtatttg ctggatcttg ccgatcggat tcaggcagta ccgaataatc gcggggcggg
     6961 cttcttctat tgggaacctc tttggtacaa cggaaatgtg agttgggcaa cacaagcagg
     7021 tatgaattat ctcagtgttc aatccactat gggaaacgaa tgggacaacc aagcgatgtt
     7081 tgattttgct ggaaacgcct tgccaagctt gcgtgcgttt aaacaagcag gggcgcaaat
     7141 aaatctagtt aaaaatgcaa gtttcgaaag cgatggacca acgaccagtc cttccaattg
     7201 gcaaacctgg tttcaacaag gaaccgctaa gggtacgatc aaaacggaag cgggcacttt
     7261 ttttggggac tacaaactaa ctttttggca ggatcaagca tacgaggcat cagcttatca
     7321 aaatgttagt ggcataagca aaggaactta cacattgagt gcctatgtaa tgtctggggg
     7381 agagtttgat actaatcagt tatatatcaa aaattatggt ggagcggaac taaatactgc
     7441 ggttgttaaa acaagtgaat ggacaaaaat tacaatcaaa gacattccaa ttacaacagg
     7501 tgtttgtgaa attggggttt atagcaaggc ttcaggaaat gcttggacga acgttgattt
     7561 tgtttcattg gtgaaacaat agactgcgga tcgtttcgat tcaagatcgg aggttccctt
     7621 cagccacatc gttagcgtag aaggcaggaa tcgaacctgc acctcgcctt tgccaaaaat
     7681 aaccagaaac gatttccagt catttttacc aaaacgagta aagcaccaga cttcatctac
     7741 actttgtatt atgctaaaga gagttcaacg atttcgtaag aagcaatgaa aaaattgcaa
     7801 aaagttgtgc agataacata aagtttgttt aagaaagggt aaccttagaa ttccgtcgtg
     7861 aatatgattt gagcacttag atggatagaa aaaagttctc ggaaaacagt tcgtttttcg
     7921 agaacttctt tttatgataa gaatgaaaca aattaactta tattatagtc tcttttggta
     7981 gggaaccatc ttatttttaa tgaaaatcaa gcattttagc tattgaagaa tgttgattta
     8041 tagggattcc ccctccatat tatgttggct ctttattccc actccactgt tgctggtggt
     8101 ttagaagtga tgtcatacac gatacggttg acgtgggcta cttcgtttac gatacgtaca
     8161 gagatttttt gcaagacttc ccatgggata cgggcaaagt cagcggtcat tccgtcgatt
     8221 gaagttacag cacggatacc gactgtgtag tcgtaagtac gtccgtcacc cataacaccg
     8281 acagagcgga tccctggaag aacagtgaaa tattgccaga tatcacggtc aagtccagca
     8341 gctgcgattt cttcacgcaa gatagcatca gattcgcgta cgatttctag cttgtcttca
     8401 gtgatttcgc caagaacacg aataccaaga ccaggaccag ggaatggctg acgccaaacg
     8461 attgaatcag gcatgcctaa ctgagtccct aattcacgga cttcgtcttt gaataatgtg
     8521 tttaatggtt cgatcaattc aaattgcata tcttctggca gtccacctac attatggtga
     8581 gatttgatcg tctgtgctgt ttctgtacca gattcgataa cgtcagtata caacgttcct
     8641 tgtgctagga aagagacgcc ttcttcacca gcaagttttg ttgcttcgtc atcaaaaaca
     8701 tatacgaatt cattcccaat gattttacgt ttttgttctg ggtcagatac acctttcaac
     8761 ttgtctaaga agcgatctct tgcattgact ttgatgatgt tcaagccgaa ttttccgcct
     8821 agactttcca ttacttgttc cgcttcgcct ttgcgtagca gaccgtggtc aacaaagata
     8881 gatgtcaatt ggtcaccgat cgctttttgt aaaagaaccc caacgacact agaatcaacg
     8941 ccacctgaaa gaccaagcaa gacttttttg tcaccaactt gttcacggat tttttctact
     9001 tgcatatcga taaagttatc cattgtccag ttaccttcac agtgacaaac atcaaaggca
     9061 aagtggcgca gcaattcgat tccgtattcg gaatgacgta cttctgcatg gaattggata
     9121 ccataaaaat tacgtgttgt gtcttggata gaagcaatcg gacaatcttt gcttgttccg
     9181 actgtttcaa agccttcagg aactttagtt accaagtcac catggctcat ccatactgtt
     9241 tgttgttttg gtgtttcttt aaacaagaca gcttgatcgc ttgttacttc taacaaggct
     9301 ttcccatatt cgcggttttt agcaggctct acggaaccgc caagtttgta cgtgatcaat
     9361 tgcataccat agcaaatccc taatacagga atacctaaat tgaagatctc aggatcgatc
     9421 ccaaatgcat tctcatcata tacactgttt ggtccgccag aaagaatgat tccctttgga
     9481 ttcatcgctt ttacttcttc tgcagtaatg cggtggctca ataattcgga aaagactcca
     9541 agatcacgaa tacgtcttgt gatcaattgg ttatactgac taccgtagtc taatacgata
     9601 atcttttcaa cgttcgtcat atcggcaacg tttgtcaaat ttattacccc tatcccttta
     9661 aattttatct caaaatagtc aaactatttt aaaatgctaa tattttcgca tgaaaatcga
     9721 atcgatgata tgattttcag ttttatggag aattatatcg gctcttcctc gtgtaggcaa
     9781 gatatattcc tgtaaatttt tcaaattcac atttttccat acatcttttg ccattttaaa
     9841 ggcttcttca cgatctccga tagcaaaacg ataataataa ttctctggat tttggaaagc
     9901 cgtatccagt aacgcaccaa aacgatccaa ataccatttt tcgatcagca cgggatcggc
     9961 atcgacatag acagaaaaat caaaaaagtc gctgacgtag atttgctgat tcgcaggtaa
    10021 ttgaagcgta ttgatcccct caacgatcaa gatatcaggc tgctggatca attcgtactt
    10081 gtctggaatg atatcataca catcatgtga gtaaacaggt acttttatct cttcctgacc
    10141 agattttaca cggttcaaga aatcgatcag catctccata tcataacttt caggaaatcc
    10201 tttacggtcc atgattccac gctcttttaa tatacgattt ggataaagaa atccgtcagt
    10261 cgtgatcagc tgaacgtttc ttcgtttgaa ggtacgagac aaaatcgtct gcagcagtct
    10321 ggctgttgta ctttttccaa cggctacact tccggcgatc ccgataataa aaggcggtac
    10381 ttctacgtat ttatgaagaa ataaaccttt actcaagaac aaagattcaa attctttcat
    10441 ataaagataa atcagatgag caagaggtac atagatatct cgaacatcct gcatggaaat
    10501 gcggtcattc aaacttttga tattatctag ttcatcttgt gtcaaaggtg cttgcccatt
    10561 acgataaaat tctcgccatt cttctcttgc cacacggtag tagttcattg gatcattcat
    10621 attagcctcc tgcgttctcc catcaatgga actattttaa cacaaaataa acgcaaaccc
    10681 caccacttaa gagagattgc cgatcttttt gtataatctt tataatagaa agaaacggag
    10741 tcttattctt gacttgcgca cggatacata aaaggataga ctgttgtcat gaaagaaatg
    10801 agaaggatgg aagacatgac aaatcattac tattctgaac gcccagatac ggcgcacgat
    10861 tttgaacaat gggcttttga gttaaaaggc agaacctttc aatttgtaac agattctggt
    10921 gtattttcac gtgaaacagt ggattatggt tcacgtgtcc tgattgatgc tttcgattgg
    10981 gaagagttac ctgatgaagg aaagatctta gatgtgggat gcgggtatgg accgattggg
    11041 ttagctttgg catttgcgac acagcggttt gttgaaatgg tcgacattaa tgaacgagcg
    11101 gtaagtctag cgcaaggaaa cgcaaagcgt aaccaaatcg aacatgttga tatccatcaa
    11161 tcgaatatct atgaagcagt tcatgaagaa acctatgcag ctatcgtaag caatccgccg
    11221 atccgtgctg gaaaaaaagt tgtacatgaa attttgacag gcgcatatcc tcggttgaag
    11281 aagggcggta cactgacaat tgtgatccag aagaaacaag gagcgccgag tgcgcaaaag
    11341 aaaatggaag aaacatttgg taatgcagaa atcgtcacaa aagataaagg atattatatc
    11401 atcaaaagtg tgaaagaata actagctgaa aaatagtgga gcgacgaaag agtggaactg
    11461 ggcgtgcatg tacgggtacg tatcagttct ttttttgttg agaagttttt aaaaacaaag
    11521 aactattttt tcttcaaaaa gaaatagtag gaacagagtc gttgtttttt acgataaaat
    11581 aaataagata cagaacttgt atagaaggct gccaagtcgg tagataaagg aggtttttat
    11641 caaaaaacat actttccttt tttctctatt cttttcttgg gggttttggt acactgatag
    11701 ggaatttaga tagaagggag ccaataggat gaagctgacc atcagagata tcgcagaaat
    11761 ggcaggcgta tcagtaacaa ctgtctcaca gattttgaat aacaaaggaa gcagatttag
    11821 tgataagaca agaaaaaaag tgttgtcgat cgtgaatgaa catcattata agcccgatta
    11881 tttcgcttca aatgtgatca atcgacattc aaagaccatc ggaatgatcg tccctgatgt
    11941 cactgatttc ttcttctcca aactgatcga aggtgtagag agctatttga atcctttggg
    12001 ctatgtgatc atgttatgca attctcgtca tagccaagaa aatgagatca agtatttaca
    12061 agagctttcc caccgctcag tggacggaat cttgctggcc acaccaaata ttttacctga
    12121 gaaatatgca ttagacagcg gtttttatca aaaaatgcca atcattttaa tcgatcgcgg
    12181 attgaatcgt cgggataacg gacggcttat cgtaaaggaa tatgagggcg cctatcaagc
    12241 tgtttcgctg tttattgaaa atggccatac gaagatcggc atgctgaagg aaaccactgg
    12301 atactatcaa ttagaagaac gtttcaacgg atatcgtcat gcgctgaaag atcatggcct
    12361 gccttttaat gggaaatttg ttgaacatgg agagttgact gtccaaggtg gatatactgc
    12421 atcaaaaaaa ttactcaaac ataaagatat cacggctatt ttttgcggga atgatgccat
    12481 ggcgatcgga tgctatcagg cgatcgatga attgggcaaa aaaatcccag aagatatctc
    12541 agtcatagga ttcgatggat taaagctgtc agaatacatg attccgcaat tgacgacagt
    12601 gcagcagcca agttttgata ttggatttta tgctgcacgt ttcctgatcg atacgatcga
    12661 atttcctcaa agaaaagttc cgaacaaagt gttcgaaaca aaattaatta ttcgtgagag
    12721 cgtaaaagta ttgacaaagg gctaactcgg cggtatattg cttatgtaag catttacaaa
    12781 ggctagttag tgcttttttt atttttagta aaccagttta ctaaagtggt ttacctttcc
    12841 taaaaataat gtataatacc gaatgaagac aaggaggatc aggcaatgcg aatggtggat
    12901 ctgattgaaa aaaagaggga tggaaacgag ctgtcgaaag aggaaatcga gtacatcgtc
    12961 acgaactata cgaatggaaa gattccagat tatcaagtca gtgcattatt gatggcaatt
    13021 ttttatcaag acatgacaaa tgaagaaatc acgaacttaa cattggcgat tgctaattct
    13081 ggtgatgtca tcgatttgag ttctcttgaa ggaataaagg tagataagca ttcgacaggt
    13141 ggcgttggtg acacgaccac attgatttta gcccccctag tagctagtgt cggagtgaca
    13201 gtggctaaaa tgtctggacg cggtctaggt tacacaggtg gtacactaga caagctagaa
    13261 gcaattcccg gatttcagat cgagctttcg gatgaagaat ttgtacgcat tgttaatgaa
    13321 agtaaagtag cggtaattgg tcaatcagga aacttggcac cggcggacaa aaaattgtat
    13381 gccttgcgtg acgtcacagc gaccgttgat tctcttccgc tgattgccag ttcgattatg
    13441 agtaaaaaaa tcgcggcagg agcagatgca atcgttttgg atgtgaccac tggagacggt
    13501 gctttcatga agaacatcga agatgcaaga cgtctggcaa agaccatgac gagcattggg
    13561 aaactggcta accgagaaac agtggcggtt atctcagata tgtcagaacc tcttggcgaa
    13621 gctatcggaa atagtctaga agttgtcgag gcaatcgaaa cattacaagg aaatggtcca
    13681 gaagatctag tagaaatgtg ctacgcctta ggaagccaaa tggttgttct tgctggcaaa
    13741 gctaagacaa ccgatgaagc aagaacgttg cttcaagaag ctctggaatc aggtaaagcg
    13801 ttagcaaaat tcaaagaaat gatccaaaat caagggggag acccagcgat cgtggaacat
    13861 ccagaacgaa ttttgactgc acgctacaca atggagcttc ctgctaaaca aagcggagtt
    13921 gtgtcaaaaa tcgtggcaaa tgaattagga attgctgcaa tgatgctagg tgctgggcgt
    13981 aaaaccaaag aagaagatat tgatcacgct gttggattga aactgcataa aaaaattggt
    14041 gacactgtga caaaaggtga gtcactattg acgatctata gtaacgacga agaaatcact
    14101 tctgtcattg aattattata taaaaatatt gaaattggtg aatcggcaat gaagccaacg
    14161 ttgattcatg acatcatcac agaataaagg aaaaaagcgg actttaccgt tttaaagaaa
    14221 aggggaaaag aaatggaact aaaccgaatg atcgaccata ctattttgaa agcagacgca
    14281 tctaaagaag ctgtgatgca aattatcgaa gaagcaaaaa aataccattt ttattctgtc
    14341 tgcatcaatc cagcatgggt tgcgctagcg aaagaacaat tacaaggtga gccagtagcc
    14401 gtatgtacag taatcgggtt tcctctagga gcaaacacac ccgagacaaa agcatttgaa
    14461 acaacagatg ccatcaataa cggtgcagat gaagtcgata tggtcatcaa tatcggtgct
    14521 ttgaaatcaa aggatgatca acaagtccaa aaagatatcg aagcagttgt tgaagcagca
    14581 aaagatcgtg cgcttgtcaa agttattatt gaaacatcgt tattagatag agaagaaatc
    14641 atccgagctt gcgaaatagc aaaagctgca ggcgctgatt ttgtcaaaac atcaactggt
    14701 ttctcaacag gcggagcaaa ggtagaggat gtccgtttga tgcgtgaaac tgtaggacca
    14761 gaaatgggtg tcaaagcttc cggcggtatc cataacgaag aagaagcaat ggcaatgatc
    14821 gaagctggtg ctacaagaat cggaacaagc gcaggagtag caatcgtatc tggttcaact
    14881 ggtgaaggct attaattttt tcaaaggaga agctcatggc agaactcaaa caagaatgga
    14941 tcaacacagc tattgaagcg ttagataaag cctatgttcc ttattcccat tttccggtag
    15001 gcgcttgtct cgtaacggaa tcaggcaaga tttatcaagg catcaacatt gagaatgcat
    15061 catttggatt aacgaattgt gccgagcgca cagctttttt caaagcagta tctgaaggag
    15121 aaaaaacatt tacgcatcta gtcgtagcgg ggcacacccc cgatcctatc tctccttgtg
    15181 gtgcatgtcg acaagtaatg gcagaattct gttcccctga tatgccggtt acgctagtag
    15241 gagacaatgg tgtgacgaag gcaacgaccg ttcgtgcact gctgccttat gcatttactg
    15301 aaaaagatct ataactcatg ttcattcagt cagctgactg ttgaaaaata aaacaattat
    15361 tttaggaggc tttacagaaa tgaaaaaagc aaaattattt ggtttaggtg ctgtcgcact
    15421 agcagcaggg ttatttttag gtgcatgcgg caacaacggc tctactgatt catccggtgg
    15481 caaaagctca tcagacacaa caactgcagc attgatcaca gacacaggtg gtgtagacga
    15541 ccgttcattc aaccagtcag cttgggaagg actagaaaaa tgggggaaag atcaaggact
    15601 ttcacgagga aatgatggct tccaatattt ccaatcttca aatgaatcag attacatccc
    15661 aaatattgac caagcattga atgctggatt caaaacgatt tttggtattg gttataaact
    15721 aaaaccagcg atccaagaac aagcaacaaa caatacaggt acgaactttg ttattatcga
    15781 tgatgtcatc gatggattag ataacgttgt atctgcaact ttcaaagaca acgaagcttc
    15841 ttatcttgca ggtgttgcag cagcttatac gactgaaaca aacgtagtcg gctttatcgg
    15901 cggtgtaaaa ggtgaagtaa tcgatcgttt tgatgctgga ttcaaagccg gtgttgatgc
    15961 aggtgcgaaa gaattaggaa aagaaatcaa agtattgaat caatatgctg gtgacttctc
    16021 tgctccagat aaaggacgtt caattgctca aggtatgtat gcacaaaacg cagatatcat
    16081 cttccatgct tcaggcggta caggtaacgg tgtattccaa gaagcaaaat cattgaacga
    16141 atcaggcgac aaaaaagttt gggttatcgg tgttgaccgc gaccaatcag acgaaggcga
    16201 atacacatta aacggcgaaa agaaaaactt cacattgaca tctacactga aagcagttgg
    16261 aacagtagta gaagatttgg cacaaaaatc agcggacggt aaattccctg gcggcgaaca
    16321 cactgtttat ggattgaaag aagacggtgt tggtctaaca gaaggacaac tttctgacga
    16381 agcgaaaaaa gcagttgatg aagcaaaaga aaaaatcatt tcaggtgacg taaaagttcc
    16441 agaaactcct gaagaaaact aaaaccattt ctatagcctt gttagacaca tgtttgacaa
    16501 ggctatggtt tgattagaac aagaattgtt catttatcca agctgccttg tgacagtttg
    16561 gataagggaa caactccctg aagtaatgtt tgtttcaaaa gaagaacgct cactaagcgt
    16621 gaatcttagt caatcgttgc cctagataga aatcgttagg atgtgaaaaa aaggtggttc
    16681 aagaaaatat tgtcatagaa atgcagaata ttaccaaaga atttggaaca ttcaaggcaa
    16741 atgacaacat caatttacaa gtaaaggcag gagaaattca tgcgttgctt ggagaaaacg
    16801 gtgctggcaa atctacattg atgaacgtgc tttccggatt attagagccg acatcaggga
    16861 aaattttgat gcgtgggaaa gaagtaaaga tcacaagtcc gaccaaagcc aaccaattag
    16921 ggattgggat ggtccatcag cactttatgc ttgttgatgc ctttactgta acagaaaaca
    16981 tcgtgttggg aagcgagcct agtcgcgcag ggatgcttga ccataaaaaa gcacgaaaag
    17041 agatccaaaa agtttctgaa cagtatggat tgtcagtcaa cccagatgct tacgttcgtg
    17101 atatttcagt tggaatggaa caacgggtag aaattttaaa aacactttac cgaggagcag
    17161 atgtactgat ttttgatgag ccgacagctg tattgacccc tcaagaaatt gatgaattaa
    17221 tcgtgatcat gaaggaatta gtcaaagaag gcaagtcgat cattttgatt acgcataagt
    17281 tagatgaaat caaagcagta gctgaccgct gtacagttat ccgccgtgga aaaggaatcg
    17341 gtacagtcaa cgttaaagac gtaacctcac aacaattagc cgatatgatg gttggaagaa
    17401 cggtttcatt caaaacgatg aaaaaagaag cgaagcctca agaagtcgtt ttgtctattg
    17461 aaaatctagt ggtgaaagaa aatcgtggat tagaagccgt gaaaaacttg aacttagagg
    17521 ttcgtgctgg cgaagtactt ggtatcgctg gaatcgatgg aaacgggcag tcggagttga
    17581 tccaagcttt gactggtttg cgaaaggcag aaagcggaca tatcaagcta aaaggggaag
    17641 acatcactaa taaaaaaccc cgaaaaatca ctgaacatgg tgtaggacac gtgccagaag
    17701 accgtcataa atacgggtta gtcctagata tgacattatc tgaaaatatc gcactacaaa
    17761 cctatcatca aaaaccttac agtaaaaacg gcatgctgaa ttattcagta atgaatgaac
    17821 atgccagaga attgatcgaa gaatatgatg ttcgaacaac gaatgaactt gttcccgcaa
    17881 aagctttatc aggcggaaat cagcaaaaag caattatcgc tcgtgaagtc gaccgagatc
    17941 ctgatctgtt gattgttgca aatccaactc gtggactgga tgtaggtgcg atcgaattta
    18001 ttcataaacg tctgatcgaa caaagggaca aatacaaagc agtgttactg atcagtttcg
    18061 aattagaaga aattttaaat gtttcggatc gtattgctgt tatccatgaa ggagaaattg
    18121 tcggtatcgt tgatccgaaa gaaacatctg aaaatgaatt aggtctattg atggcgggct
    18181 ataccttaga agaagcacga aaagagttag aaaagggggc tggcgcacat gaatgatcgc
    18241 agcgaaaaat tccgtaattt attagtgcct gtactttcag ttgttttagg attgatctta
    18301 ggcgcgatat tgatggctgc ttttggtttt cagcccatcc aaggttattc ttctatgcta
    18361 gatgcagccc ttggttcgca aagagggata ggtgaaacgt taaggcaagc aacgccgttg
    18421 atttttacag cattaggttt ttctgtagct aactcagctg ggtttttcaa tattgggctt
    18481 tctggacaag cactttgcgg gtggatcgtc agtgtctggg tggcacttgc gtttccagat
    18541 ctgccaaaag ttgttttgct tccgttatgc gtgatcttag gtgccttagc tggtgcgatg
    18601 gcagcggctg ttccaggttt acttcgagca ttttttggaa caagtgaagt gatcgtaaca
    18661 atcatgatga actatatctt attatacgta agcacatttg ctttacatga agtgatgcct
    18721 gaaagctatc gctcaagttt ggactcttcc aatatgatca gtcaaaatgc ctcactgcgg
    18781 ttaccatggt taacacaagc ttttggcggc tctcgcgtaa actcaggatt atttttagca
    18841 atcatcgcgc ttattttcgt ttggctgctg atgaagaaaa cgactttagg atttgaaatc
    18901 cgttctgttg gattgaatcc ttttgcttct gagtatgcag gaatgagtag taaacgtacg
    18961 atcgttttgt cgatggtcat ttctggcgct ttggcagggc ttggcggtgt ggttgaagga
    19021 ttaggaacgt atcaaaactt ctttgttcaa acgacttcgc tatctattgg atttgacgga
    19081 atggctgttt ccttgcttgg ttctggatca gcaatcggta ttcttttatc agcgatcttg
    19141 ttcagtattt tgaagatcgg tggacttggc atgcagacag gagcaggtgt tccgtttgag
    19201 atcgtcaatg tatcgattgc tttgattatc ttctttgtcg ggatcaactt cctgattcgt
    19261 ttcgtcatgg ctaaagtctt ccaagggaaa aaacaagaag agatcgttgc gacaatagaa
    19321 tcgaaaccaa cgaatcaaaa ccatgaaggg ggcgaactat aatgtcgact atcgaattaa
    19381 ttgccttgat ccttacttct acactggttt attcaacacc gctaattcta acgtcactag
    19441 gcggaacttt ttctgaacga agcgggatcg taaacgtagg gctagaggga atcatggtca
    19501 tgggtgcttt tagttccatc gttttcaact tgacgatgag tccatcactt ggttctatga
    19561 caccatggat cgctatttta gtgggtggcg tcgtaggtat cctattttct ctgattcacg
    19621 cagtagcaac agtcagtttg cgtgctgacc atattatcag cgggacagta attaatctga
    19681 tggcacctgc tttaggtgtt ttcttgatca aagcatggta tggtaaaggt caaactgata
    19741 acatcacgca gaatctgggg tatttttctt tcccaggatt agcagatatt ccggtcattg
    19801 gaccaatttt ctttaaaaat acattcttac ctgcttattg tgctattttg attgctgtcc
    19861 tagcttgggt gatcatcttc aagacgaaat tcggcttgcg cctccgttca gttggtgaaa
    19921 atcctcaagc tgccgacaca ttaggtatca atgtatatgc tatgcgttat tcaggcgtac
    19981 tgatttctgg cttgttaggc ggtatgggcg gtgctgtttt tgctcagtcg atttccggta
    20041 acttttcagc tggtacgatc gttggacaag ggttcatctc catggctgct atgatctttg
    20101 gtaagtggaa tcctttaggg gcaatgggtg cctcattgtt ctttggtttt gctcaaagct
    20161 tgagtatcat tggcgcacaa ttaccagtta tttcttcgat tccgccagta ttgctgcaaa
    20221 ttgctccata tttgctgaca atcattgtgc tggttgtttt cttagggaaa gcttctggtc
    20281 caaaagcaaa cgggaagaac tatatcaaat caaaataatc ttttagcgaa taaaaagggt
    20341 gggttacggg aagtgttttt cgtctcgccc tttttgtttt aaaggcaata aaaataaaaa
    20401 gaaagaagga ataatcatgt ttaaacgcgt acatctgatc gtcatggatt cagtaggaat
    20461 tggagaagca ccagatgccg ataagttcgg tgatgttggt tctgatacat taggacacat
    20521 tgcgaaagaa gcaggattga acgttccaca tttagagcag cttggtttag gaacgatccg
    20581 tcctcttgaa ggtgtggaat cagtgaaaga tcatcaaggc tatgcgacga aattagaaga
    20641 agtttctgta gggaaagata cgatgacagg ccactgggaa atcatgggat tgaatatcaa
    20701 aactccattt cgtgtgtttc ctaatggatt ccctgatgat ctgatcaaac aaatcgaaga
    20761 attttctggc cgtaaagtcg tatgtaacaa accttatagc ggaacagctg ttattgacga
    20821 ttacggtgaa caccaaatga aaacaggcga tttaattgta tatacttctg ccgatcctgt
    20881 ccttcagatc gcggcacatg aagaaatcat tcctttagaa gaattatatc ggatttgcca
    20941 attcgttcgt gatataacaa aagatgatcc gtatatgatt ggccgaatca ttgcacgtcc
    21001 gtacgtgggt gaaccaggag acttcacacg aacaagcaat cgccatgatt atgcgttgaa
    21061 tccattcgga cacacagtcc ttgattcttt gaaagaagca ggaaaggacg tgattgctgt
    21121 tggaaagatc aacgatattt tcaacggaca aggaatcact gaatctgtcc gcacgaaaag
    21181 caatatggat ggcgttgacc agctgctgaa agttatgaaa aacgattttt caggaatcag
    21241 ctttaccaat ttagtagatt ttgatgcatt atacggtcat cgtcgggatg tagtcggcta
    21301 tgcgcatgcg attgaagaat ttgacttacg aattccagaa ttgcttgaag cgatggaaga
    21361 agacgacttg ctaatgatta cagccgatca cggaaacgat ccgacgttta ctggtaccga
    21421 tcatacgcga gaatatgtac cgttattggt ttacagtaaa aaaatgaaag aacaaggggc
    21481 attgcctcaa ggatattatt cagatatttc agcaacgatt gctgagaact ttagtgtttc
    21541 ttcgactgaa aatggcgaaa gcttcttgaa attattaaaa taatcttttt aaataggaga
    21601 aagcaaatga ccaagttaag cgagatgtta caagaaacga cccagtttat ccttgataaa
    21661 ggagtaaaag aaattgattt tggtttgatt ctaggttcag gattaggtga attagcagat
    21721 gaaatcgaag atgctgtaat gatttcttat gatgagatcc cgttctttcc tgtttcgaca
    21781 gtagttgggc atgcaggaaa gctagtttat gggtctctgg caggcaagaa agtgttagcc
    21841 atgcaaggga gattccacta ctatgaaggt cattccatgc aaacagtgac ctatccagta
    21901 cgtgtgatgg ccggactagg ggcccattca gtcattgtga ctaatgcatg tggtggtgtc
    21961 aacgaatcat ttgtaccagg agatttgatg ctgattaccg atcatatcaa ttttactggt
    22021 gaaaatcctt tgatcggtcc gaatgaagat gcaatgggcc ctagatttcc agatatgagt
    22081 caagcttata cgcctgacta tcaagcaaca gcaaaagcag cagcagaaaa actaggtttg
    22141 gatcttaaag aaggcgtata tatgggctat tctggaccaa cttatgaaac gcctgcggag
    22201 atccgaatgt ctcggacaat aggagccgat gcagtaggga tgtcgactgt tccagaagtc
    22261 atcgtagcag ctcacagcgg attaaaagtg ttaggaatct cttgtattac caacttagca
    22321 gcaggtatgc aagcgaatct gagccatgaa gaagttgtag aaacaactca gagagtcaaa
    22381 caatctttta aagcattggt taaagaaaca ttgacattat tgtaatagga ggaacaataa
    22441 aatgagtgtg catattgaag cgaaagaagg cgagatcgcc gataagattt tacttccggg
    22501 agatcctttg cgagctaaat atattgcgga gacttttttg gaagatccag tttgttacaa
    22561 tcaggtacgc ggtatgttag gctttaccgg caaatataaa ggagaacgta tttctgttca
    22621 aggaacagga atgggaatgc catcagccac catctatgcc catgaattga tccagtcgta
    22681 tggtgtaaag aaattgattc gagtaggtac atgcggtgca ttgtcaaaag acgtccatgt
    22741 tcgtgattta gtactagcac aaggtgcagc gaccagttct tcgatgatcg aaaaaaattt
    22801 ccaagccttt cattttcctc caatcagtga tttcaatcta ttactgaaag catatgagat
    22861 agctaaagaa aagggctata cggttcatgt aggaaatgtt ctttcggaag attctttcta
    22921 caaagatgat ttgacagaga cgttccaatt agctgaactt ggcgttttag gagtggaaat
    22981 ggaagcagcg gctctttatt acttaggcgc gaaataccat gtgcagacat taagtttgat
    23041 gaccgttagc gaccatttga tcaccggtga agaaacaaca gctgcagaac gtcaatctac
    23101 gttcaatgaa atgatcgaag taggattgga aacagcgata gcagaataag aaggaaatga
    23161 aaaagctgcc ctgcattaag taataagaac atccaagcaa aaatagctcc tcatgttttt
    23221 gacttggctg gacttattat tgcaaatgta agcttttgaa caccgtttac caagaggttg
    23281 tgaatctgcc gtttaactct gagtcacaag gaacaatttc aaaaaacagc ttttcatgtt
    23341 tgatgaatat tgtgacttgt gaccgagaag ttggcaggag aaaaccgttt atacgaaaaa
    23401 agggactgtg acaaatcgtg tcacagtccc ttttttatag tgttttttat aggtagtatt
    23461 ttttagcaac tgttaagtca tcatttaatt cataaacaag tggttgacct gttggaattt
    23521 caagatccat gatgtcttcg tcagagatac cttcgatatg tttagctagg gcgcgtaatg
    23581 agttaccatg tgcagctact aaaacagttt tgttgtctag taatgctgga gcaatttcat
    23641 cttgccagaa tggcaaagcg cgttctaatg tcactttcaa gttttctcca ccaggaatat
    23701 cgcgtttgtc taacattgcg taacgacggt catttgctgc agaaccttcg tcagtttctt
    23761 ccattaatgg aggaagagta tcgtaagaac gacgccagat gtgcacttgt tcgtcgccgt
    23821 atttttcagc tgtttctttt ttgttcaaac cttgtaattt accatagtga cgttcgttta
    23881 agcgccatga tttgatttgt ggtacccaaa gttgatctga atattctaag atcaagttac
    23941 atgttttgat tgcacgagtt aaaacagaag tgtaagcaac atcaaattcg attccagctt
    24001 ctttgatttt gcgtccgcct tcttttgctt cttcgattcc ttctggtgct agatttacat
    24061 cagcccaacc agtaaattgg ttcaatgcat tccattcact aagaccgtga cgagaaaaaa
    24121 ctaattttgg catgtttagc tctcctttta agataggttt atatttctga aattttcagt
    24181 tctgttttat tgtacaattt tttggaagag atttccatcg aaaaattaga taagattcta
    24241 tgagaaaaca agtagagaat ataaaaaaac gaacagtgcc cacgcaactg ttcgttttta
    24301 ggtattagat gcataattgc tcgatttctt tttcaggtaa attgatatat ttataacatg
    24361 ttccaactga aacaccgcat tttgaagaaa taaattggat cgtttctttt ttttcataat
    24421 agagacgtcg gatttttttg atcgtttttg catcgatttt tggtcttccg ccgatttttc
    24481 cttttttacg agcttcatct aatccaacta atgtacgttc tttgatcaat gcgcactcca
    24541 tgtttgctag gttttccatt aattggaaat agatttttcc catttcgcgc gtatcgattc
    24601 cttcatcaag actaatcaag tgcagcccgc gatctttgaa taattgcgtg agttctgtta
    24661 attgccgcgt agatttgcct aatcgatgaa gttggcaaat gactagtgca tcgccttgtt
    24721 gcatgtcagc gatcactgac tccaaatgaa cagcagtaga atcattttgt tccacttcat
    24781 atgtctcatg gaaaatttct tgacaaccaa agtcagataa ttgatcaacc tgagttgtta
    24841 aatcaacgtc atttatggtt gtgcgtgcat aaccaataat tttcatggtg attcctttct
    24901 agcagtgatt ttctaactgg atataacagt aaaaatgtag cataaagtac gtcaaaaaga
    24961 tagttttttc gcaaattttc cgctgtcttt ttttattgga tgatcaagca gtttttcttt
    25021 atttacgacg gattgcgtca agaatctcaa cgcctttttc tgagccgaca actactttag
    25081 aaaccatgtt taggaaaagc ccatgttcta ctaccccaac aagttcatct aaataggtag
    25141 ctaagctttc tggctgttcg atgacttcta gatgcagatc aatgatgtaa tgaccaccat
    25201 ctgtggttaa gacttctccg ttttcgtctg tacgcaagac gggttggaat cttttttctt
    25261 caaaaatatg aagtagctgt tgggagccat aagggatcac ttcaactggg agtgggaact
    25321 tcccaagttt atcgactaat ttagaagaat caacgatcca aatcgtttct tttgagtacg
    25381 tagcgactat tttctcgaaa agcaaagcag cgccgccgcc tttgatccct tgaaaatcag
    25441 cactgatttc atcagcaccg tctattgtca aatcgactac ggggacttca tcaatactct
    25501 ttaacggtat tcctaatttt tccgcttgtt cttttgtcgc atttgaagta gtaactcctg
    25561 tgatacgtaa accttcttca cgcatacggc gaccaatctc ttctaccata taataggcgg
    25621 ttgaaccggt cccgagacct acgatcatcc cgtcctttac gaatttcgcg gcttcaatgc
    25681 cagctaattg ttttaaattc atctttgcgt ccaccttttt ttagtttgag catcgtgctt
    25741 tcctcactat tttagcgtga tttacggtaa aaagatacca ttctttttta ttccaataaa
    25801 gctttttcaa aaaaaattgc atctcttgtt gacatgtttt ttcattcgtg atattctaac
    25861 aaaggtgctt tatctacagg tttctgtgaa gaaacgtgct gactgtttat caaagcatat
    25921 ttgataagga tgcagacatt gcatttattt tttatcacaa aaaaagaacc acctggatgt
    25981 gtggaactag acgcaaatcg tgggaaggag gaaacaagga atgcctacaa ttaaccaatt
    26041 agtacgtaaa cctcgtaaat caaaggtgga aaaatctaat tcacctgctt tgaacaaagg
    26101 atataacagc tttaagaaaa ctcaaactaa cgtaaactca cctcaaaaac gtggggtagc
    26161 aactcgtgtg ggtacaatga cacctaaaaa accgaactca gctttacgta aatatgcacg
    26221 tgttcgtttg tcaaacttaa ttgaagttac tgcttatatc ccagggattg gtcataactt
    26281 acaagaacac agtgttgtat tattacgcgg tggtcgtgta aaagacttac caggggtacg
    26341 ttaccatatc gttcgcggtg ctttagatac tgctggtgtt aacgaccgta aacaaagccg
    26401 ttctaaatac ggaactaaaa aacctaaagc ttaattttaa ttattaaaca aatgaaactt
    26461 gcttctacta tatagaagta attactatga acaatatttc ggaaggagga gttacggatg
    26521 ccacgtaaag gtcctgttgc aaaacgtgat gttttaccag atcctattta taactcaaaa
    26581 ttagtaactc gcttgatcaa ccgtgtaatg gttgacggaa aacgcgggat tgcagctaac
    26641 attatctata atgcctttga cttgatcaaa gaatctacag gtaacgatcc attagaagtg
    26701 tttgaacaag caatgaaaaa cgttcaacct gttcttgaag ttaaagcacg tcgtgttggg
    26761 ggttcaaact accaagtacc agttgaagtt cgccctgaac gccgcgttac tctaggtctt
    26821 cgttgggtag taaactacgc tcgcttgcgc ggtgaacaca caatggaaga acgcttagca
    26881 aaagaaatca tggatgctgc aaacaacaca ggtgcttctg ttaaaaaacg tgaagacact
    26941 cataaaatgg ctgatgccaa ccgtgcgttt gctcactatc gctggtaaga ttctctctgt
    27001 ttggtcggct tttataagtc gacctcagat agtgtcctta tttttagcaa ctagatagta
    27061 aagagaggag aaacaaggag aatgaccaga gaattttctt tacaaaacac tcgtaatatc
    27121 ggtatcatgg cccacatcga tgctggtaaa acgactgcta ctgaacgtat cttgtactat
    27181 actggtaaaa tccataaaat tggtgaaact catgaaggag cttcacaaat ggactggatg
    27241 gaacaagaac aagaacgtgg tattacgatc acttctgctg ctactacagc acaatggaag
    27301 ggtcaccgaa tcaacatcat cgacacacca ggacacgtag acttcacagt tgaagttgaa
    27361 cgttcattgc gtgtacttga tggtgctgta actgttcttg atgcacaatc aggtgttgaa
    27421 cctcaaactg aaacagtttg gcgtcaagca acaacttatg gtgtacctcg tatcgtattt
    27481 gcaaacaaaa tggacaagat cggtgctgac ttcttatatt cagtatcaac tttacatgac
    27541 cgtttgcaag caaacgctca cccaatccaa ttaccaattg gtgcggaaga cgacttcaca
    27601 ggaattatcg accttgtaac aatgaaggct gaaatgtata caaacgatct tggaacagag
    27661 atcgaagaaa cagaaattcc tgaagaatac cgcgaattag ctgaagaatg gcgcgaaaaa
    27721 ttggttgaag ctgtagctga aactgatgaa gaacttactt tgaaatactt agaaggtgaa
    27781 gaaatcactg aagctgagtt aaaagaaggt atccgccgcg caacagttaa cgtagaattc
    27841 tacccagtac tatgtggttc agcatttaag aacaaagggg ttcaattact attggacgct
    27901 gttcttgatt acttgccatc accgttagat atcccagcta tcaaagggat tgaccctaaa
    27961 actgatgaag aagtagaacg tcctgcagac gacagcgctc ctttctcagc tttagcattt
    28021 aaagttatga ctgacccatt cgtaggtcgt ttaactttct tccgtgtgta ctctggtgtt
    28081 ttacaatcag gttcatacgt acaaaacgca acaaaaggca aacgtgaacg tgttggtcgt
    28141 atcctacaga tgcatgccaa ctctcgttca gaaatctctg aagtttacgc tggagatatc
    28201 gctgctgctg ttggtttgaa agacacaact actggggaca cactatgtga tgaaaagagt
    28261 ctagttattc ttgaatccat ggaattccca gaaccagtta tccaagtcgc tattgaacct
    28321 aaatcaaaag ctgaccaaga taaaatgggt gtggctttac aaaaactatc tgaagaagat
    28381 cctactttcc gtgctgaaac aaacgtggaa acaggcgaaa caatcatcgc tggtatgggt
    28441 gaacttcact tagatatcat cgttgaccgt atgcgtcgtg aattcaaagt tgaagctaac
    28501 gtaggtgcgc ctcaagtatc ttaccgtgaa actttccgtg ctggcactca agccgaaggt
    28561 aagtttgtac gtcagtctgg tggtaaaggt caatacggtc acgtatggat tgaatttaca
    28621 ccaaatgaag aaggtgctgg tttcgaattt gaaaatgcga tcgtcggtgg ggttgttcct
    28681 cgtgaataca ttccagctgt tgaaactggt ttgaaagatg ctatggaaaa tggtgtctta
    28741 gctggctatc cattagtaga tatcaaagct aaactttacg atggttcata ccatgatgtc
    28801 gattcaaatg aaactgcctt ccgtgttgct gcttctatgg cattacgtgc agcagctaag
    28861 aaagctaatc cagtaatcct tgaaccaatc atggctgtag aagttgttat ccctgaagat
    28921 tatttaggtg atgttatggg tcacgtaact gctcgtcgtg gacgtgttga aggtatggaa
    28981 gctcgcgctg gcggccaaca agttgttcgt gcgatggttc cacttgctga aatgtttggt
    29041 tatgctacaa ctcttcgttc tgctactcaa ggtcgtggta catttacaat gacatttgac
    29101 cactacgaag atgtaccaaa atcagtacaa gaagaaatca ttaagaaaaa cggcggaaaa
    29161 gctgaataat ttcttgaaac cggatagtcc tgcaaagaga ttatccaaag agactatatt
    29221 tttaatatag tttcgtgtaa aataattatc gatgatatgg gcatgtggtg taccttgtgt
    29281 acatcatata cctatcagac aaataaaaaa tatattgtat ttttaggagg acattttaaa
    29341 atggcaaaag aaaaatttga ccgttctaaa ccacacgtta acattggtac tatcggacac
    29401 gttgaccatg gtaaaactac attaacagct gcaatcacaa ctgtactatc taagaaaaac
    29461 ggcggccaag ctatggctta cgatcaaatc gatggtgctc cagaagaacg cgaacgcgga
    29521 atcacaatct caacagctca cgttgagtat gaaacagata cacgccacta cgctcacgtt
    29581 gactgcccag gacacgcgga ttacgttaaa aacatgatca ctggtgctgc tcaaatggac
    29641 ggagctatct tggtagtttc tgctgctgac ggcccaatgc ctcaaactcg tgaacacatc
    29701 ctattgtctc gtcaagttgg tgttccttac atcgttgtat tcttgaacaa agtagacatg
    29761 gttgatgacg aagaattact agaattagtt gaaatggaag ttcgtgacct attaacagaa
    29821 tacgaattcc ctggtgacga tgttcctgta gttgctggat cagctttgaa agctctagaa
    29881 ggcgacgctt catacgaaga aaaaattctt gaattaatgg ctgcagttga cgaatacatc
    29941 ccaactccag aacgtgacaa cgacaaacca ttcatgatgc cagttgaaga cgtgttctca
    30001 attactggac gtggtactgt tgctacaggt cgtgttgaac gtggacaagt tcgcgttggt
    30061 gacgaagttg aagttgttgg tattgctgaa gaaacttcaa aaacaacagt tactggtgtt
    30121 gaaatgttcc gtaaattgtt agactacgct gaagctggag acaacattgg tgctttacta
    30181 cgtggtgttg cacgtgaaga catccaacgt ggacaagttt tagctaaacc aggtacaatc
    30241 acacctcata caaaattctc tgcagaagta tacgtgttga caaaagaaga aggtggacgt
    30301 catactccat tcttcactaa ctaccgtcca caattctact tccgtacaac tgacgtaaca
    30361 ggtgttgttg aattaccaga aggaactgaa atggttatgc ctggcgacaa cgtaacaatg
    30421 gaagttgaat taatccaccc aatcgctatc gaaaacggta ctaaattctc aatccgtgaa
    30481 ggcggacgta cagttggtgc cggtgtcgtt acagaaatca aagcttaatt cacttaagtt
    30541 ttaaagtaaa gcaacattta ttcggacgta atcgctcgga acgaaggtct taggatcttt
    30601 gttccgagct tttttattta ttgcaggatc aggaaaa
//
LOCUS       DACCYH010000028        29837 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.028, whole genome shotgun sequence.
ACCESSION   DACCYH010000028 DACCYH010000000
VERSION     DACCYH010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.028"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..628)
                     /locus_tag="IVY85_001194"
     CDS             complement(<1..628)
                     /locus_tag="IVY85_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HAP8044223.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
     gene            complement(740..1771)
                     /locus_tag="IVY85_001195"
                     /pseudo
     CDS             complement(740..1771)
                     /locus_tag="IVY85_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(1853..2542)
                     /locus_tag="IVY85_001196"
     CDS             complement(1853..2542)
                     /locus_tag="IVY85_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8044224.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(2648..3016)
                     /gene="rplL"
                     /locus_tag="IVY85_001197"
     CDS             complement(2648..3016)
                     /gene="rplL"
                     /locus_tag="IVY85_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP8044225.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(3069..3572)
                     /locus_tag="IVY85_001198"
     CDS             complement(3069..3572)
                     /locus_tag="IVY85_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP8044226.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3982..4671)
                     /gene="rplA"
                     /locus_tag="IVY85_001199"
     CDS             complement(3982..4671)
                     /gene="rplA"
                     /locus_tag="IVY85_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP8044227.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4775..5197)
                     /gene="rplK"
                     /locus_tag="IVY85_001200"
     CDS             complement(4775..5197)
                     /gene="rplK"
                     /locus_tag="IVY85_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP8044228.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            5540..6208
                     /gene="sdaAB"
                     /locus_tag="IVY85_001201"
     CDS             5540..6208
                     /gene="sdaAB"
                     /locus_tag="IVY85_001201"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP8044229.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            6221..7093
                     /gene="sdaAA"
                     /locus_tag="IVY85_001202"
     CDS             6221..7093
                     /gene="sdaAA"
                     /locus_tag="IVY85_001202"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP8044230.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(7184..8203)
                     /locus_tag="IVY85_001203"
     CDS             complement(7184..8203)
                     /locus_tag="IVY85_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8044231.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(8376..9263)
                     /locus_tag="IVY85_001204"
     CDS             complement(8376..9263)
                     /locus_tag="IVY85_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8044232.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(9340..9885)
                     /gene="nusG"
                     /locus_tag="IVY85_001205"
     CDS             complement(9340..9885)
                     /gene="nusG"
                     /locus_tag="IVY85_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP8044233.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9986..10156)
                     /gene="secE"
                     /locus_tag="IVY85_001206"
     CDS             complement(9986..10156)
                     /gene="secE"
                     /locus_tag="IVY85_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP8044234.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(10180..10332)
                     /gene="rpmG"
                     /locus_tag="IVY85_001207"
     CDS             complement(10180..10332)
                     /gene="rpmG"
                     /locus_tag="IVY85_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8044235.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            10494..11138
                     /locus_tag="IVY85_001208"
     CDS             10494..11138
                     /locus_tag="IVY85_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8044236.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(11194..11697)
                     /locus_tag="IVY85_001209"
     CDS             complement(11194..11697)
                     /locus_tag="IVY85_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8044237.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(11710..11756)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12294..13259
                     /locus_tag="IVY85_001210"
     CDS             12294..13259
                     /locus_tag="IVY85_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8044238.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(13358..14530)
                     /locus_tag="IVY85_001211"
     CDS             complement(13358..14530)
                     /locus_tag="IVY85_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8044239.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(14517..15662)
                     /locus_tag="IVY85_001212"
     CDS             complement(14517..15662)
                     /locus_tag="IVY85_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8044240.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(15676..16608)
                     /locus_tag="IVY85_001213"
     CDS             complement(15676..16608)
                     /locus_tag="IVY85_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8044241.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            16725..17804
                     /locus_tag="IVY85_001214"
     CDS             16725..17804
                     /locus_tag="IVY85_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8044242.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17801..18419
                     /locus_tag="IVY85_001215"
                     /pseudo
     CDS             17801..18419
                     /locus_tag="IVY85_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(18529..18756)
                     /locus_tag="IVY85_001216"
     CDS             complement(18529..18756)
                     /locus_tag="IVY85_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8044243.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18859..21447)
                     /locus_tag="IVY85_001217"
     CDS             complement(18859..21447)
                     /locus_tag="IVY85_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP8044244.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(21467..22126)
                     /locus_tag="IVY85_001218"
     CDS             complement(21467..22126)
                     /locus_tag="IVY85_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8044245.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(22175..22678)
                     /gene="trmL"
                     /locus_tag="IVY85_001219"
     CDS             complement(22175..22678)
                     /gene="trmL"
                     /locus_tag="IVY85_001219"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP8044246.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22899..23765)
                     /locus_tag="IVY85_001220"
     CDS             complement(22899..23765)
                     /locus_tag="IVY85_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8044247.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23874..24515
                     /locus_tag="IVY85_001221"
     CDS             23874..24515
                     /locus_tag="IVY85_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP8044248.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(24577..25938)
                     /gene="mgtE"
                     /locus_tag="IVY85_001222"
     CDS             complement(24577..25938)
                     /gene="mgtE"
                     /locus_tag="IVY85_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP8044249.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25956..26855)
                     /locus_tag="IVY85_001223"
     CDS             complement(25956..26855)
                     /locus_tag="IVY85_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8044250.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26857..27654)
                     /locus_tag="IVY85_001224"
     CDS             complement(26857..27654)
                     /locus_tag="IVY85_001224"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP8044251.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(27632..28336)
                     /locus_tag="IVY85_001225"
     CDS             complement(27632..28336)
                     /locus_tag="IVY85_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP8044252.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            28467..29042
                     /locus_tag="IVY85_001226"
     CDS             28467..29042
                     /locus_tag="IVY85_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP8044253.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            29160..29837
                     /locus_tag="IVY85_001227"
     CDS             29160..29837
                     /locus_tag="IVY85_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8044254.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8779 a         6292 c         5364 g         9402 t
ORIGIN      
        1 ttttatggct aatatatcct ttgtccccta ggatattggg aagattcgct tcttccgata
       61 aagtctctaa cactttcacg tcatggacac ctggattcgt gattgagtaa gtaatcggaa
      121 agccagtctt agtaacgatc atatgaattt taagaccata aaaataagat ttttttgttg
      181 agttatagcc aactttggct atttgattca atagttttgc ttgtctattt ctaactggtt
      241 tacataatgg actaggaaaa ctatctataa ttccgactag ttcacctttg gtgagcttct
      301 tgatgaaaaa gtatcgaata atcttgatag tgtaagctaa gtttgaactt aagcgagtga
      361 atcgactcct actaggaaag tcaccattag gaaataagac ggaacaaacc gctctatacg
      421 tggctctttg gctagtatag ccattaatta tgccccatat aacacaagca ataatcactg
      481 tatcgcgttg cttcagttga tcagtatttc ttcggttttt aatcttatct ggtacacaat
      541 tgtggtatat gttagaaaca gttctcataa tttccttgaa tgttataaaa gtatctgtat
      601 aatgttcagt atattttgat gaacgcatat gtagtcctca tttcttggga gttttgagac
      661 tactatatgc gtttttttgt tgatttacca acatgtttag acattttcct ctaggaaaaa
      721 attaactagc accacgggta aatacagtat tatttcggtt gcttgctgct tcacctagcc
      781 cttgtactcg tgtttgattg gcgacttgac caaattgatt cccgacgtct aataaaataa
      841 tagctaaaag caaaatggcc aaatggcttc cgccaaccaa taaagcaata aagcttattg
      901 tttgcataac aagtcccata tacacgattt ttctttcaga ataagaatct gccagtcgtc
      961 ccacgatagg tgcagcaact gctccagaca aaccaaaaat cgctaatatc cctgcttcga
     1021 atgttcccca attgtaggcg ggactgctga tataaaaaat caacgtagac caaaagatcg
     1081 aaaatgttcc aaacatgaaa aatccactga ctgccgcttc tcttaataga cgttgagaag
     1141 caactaaatg cggcaaactt ttaagtgaat caaggtatga aacagctgta ctactttgct
     1201 ggacagaacg cggcattttc cagtaaagca gaagtgtaag aattccgact gcacataaag
     1261 caaataaata gatcatccgc caagaaaaaa gactggcaac aataccagat accgtccgag
     1321 aaagcaaaat ccccacgagt aaaccgctaa gcaatgtacc catgaccttc cctcttgctt
     1381 ttggtcccgc tagtaccgca ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc
     1441 ctattaaaaa agaagcgcat gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca
     1501 gagagacggc agataacgct accattcgaa tgatcaattt tggacgatcc accacgtccc
     1561 ctaaaggaac taaaaacagt aaccctaacg catagcctaa ttgggtcaac atcgtcaata
     1621 tcccgactgc acttgtgcta acagataaac tagctgctac ttttgttcca atcggctgga
     1681 tgtaatacat attggcaaca acaacgccac aagtgattga taatagtaaa ataagcgaag
     1741 ttgttaaagg tgttttttca ttactcttca ttgatttccc ccatttccta tcttagttta
     1801 caacttatgg aaaagattgg aaatagaaaa atacaactat tcgccacact tattatttgt
     1861 gcttttgttt tccttgtttt ttcttgcgat ctttataaat gaagacaact aaccctacta
     1921 gcacagtaac acttgcaacc aacgtgactg cagttttgtt atatccgtga aacagagctt
     1981 ctccaccaca cgcatacaac acagacgaag ggatgactcc aatcacgatc gacaatatga
     2041 tttgctgtag aggcaatttc atcgcatctg cagcaaaatt gaccatagaa gatggaataa
     2101 ccggtatcat atagcctaac attacaccta tttttggatg tttcatttgt cggattgctt
     2161 gcacccaatg attcgtttcg gttttcttat ctaaaaattt taagcgatgc ataaggaaaa
     2221 ttgcagtgat gttaccaaga gcattaccca aaatgttgat gatcgcacct gtcagtggtc
     2281 caaagctcaa accagcaatg actccaacta ctgaagtagg aatacctgga acagcacaca
     2341 tcaccgctgt caatacgatc aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg
     2401 cgagtagctc tgccttactc gctctcggat ttaaaaataa ttggatgtct gtctgatatt
     2461 ctaaatacaa ccgatagccc actaccagta tcagaatgat tcccaacgag atcaacatca
     2521 atcggattat tttctttttc atatttcctc ctgaaaaaaa tctatattca ttaaataata
     2581 gtttaacata aaaacaaaaa agcaccgaaa tccaacatag gatctcagtg cttttaaata
     2641 gtaaaaatta tttaacagtt acagatgcgc caacttcttc aagtttagct ttcaattctt
     2701 cagcttcttc tttagatacg ccttctttaa ctggtgcagg agcgccatca actacagctt
     2761 tagcttcttt caagcctaaa ccagttgctt cacgaactgc tttgataact ttaacttttt
     2821 ggtctccagc tgcagttaat tctacagtga attctgtttg ttcttcagca gcagcagcgc
     2881 caccagcagc agctacagct acaggagcag cagcagatac gccaaattct tcttcaatag
     2941 ctttaactag gtcgttaagt tctaggatag ttgcttcttt taactcagca acgatgtttt
     3001 caatgttcaa tgccattata atttcctccg tttaattgtt cttttttatt taatgcttta
     3061 tgcggcaatt aagctgcatc tccatcttct ttgtctgcca ctgctttgac agcgtaagcc
     3121 acgttgcgga ctggcgcttg tagtacagat agcaacatag atagaagtcc ttcgcgattt
     3181 ggcaattttg ccaatgctgt gatttcttcc aatgaagaga ctttaccttc gatgataccg
     3241 cctttgattt ctaatgcttt tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg
     3301 actacatctt cgttactgaa tgcaacagct gtaggtccag tgaatacttc gtccatgcct
     3361 tcaaggccag cttttttagc tgcacgtgaa agaatagaat ttttgataac tttcatttct
     3421 acgttagcat cacgaagttg tttacgtaaa cgtgttactt catcaactgt taatccgcgg
     3481 tagtcaacga ctactactga tgcagctgct gtaaattttt cgcttacttc atcaacgatt
     3541 gctgcttttt ttgcgataat tgcttcactc atttttgagt ttcacctcct gaattttgat
     3601 ggaagagttt tttgcactga aaaggcaagt cacttttata gttactttgc tcaaccagta
     3661 ttattcatca cctattcact tcgttcatag tgattctact cgctttgctc gaaaggaaac
     3721 tcctttcgct ttgctcaacc agtattgttc atcatctctt cacttcgttc atagtgattc
     3781 acaataaaaa actctatgcc accgcagaca tagagggact attgaatata ttcaataaac
     3841 gtcctcggta ggaaattaag gcttgatgcc acctactgtc ttcggtacaa gttaattatt
     3901 aacctcaact agcttagcat gctaaactag ttgagtcaag aattattttt cagttggatc
     3961 aatcaacatt ctaatattta attagaaaga agcttggtca acatggatac caggtccaaa
     4021 tgttgttgtg atcgttacat ttttcatgta ttgaccttta gctgctgatg gtttagcttt
     4081 tagcaataca tcatggattg ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac
     4141 ttttccgatt ggtacatgga tatttccagc tttgtccaca cggtaagtta ctttaccagc
     4201 tttcacttcg tttactgctt ttgttacgtc cattgtaact gtaccagttt tagggtttgg
     4261 cattaagcct tttggtccta atactcgtcc tagacgacct acagtagcca tcatatcagg
     4321 tgttgcaaca actacatcaa agtcaaacca tccgttttgg attttttgaa ccatgtcgtc
     4381 gtcacctacg aaatctgcac cagctgcttc agcttctttt gctttgtctc ctttagcaaa
     4441 aactaaaaca gtttgtgttt taccagttcc gtttggtaat acaacagcac cacggatttg
     4501 ttggtctgct tttttagggt ctacgttcaa acggtatgca acttcaactg ttgcatcaaa
     4561 ttttgcaatg tttgtttctt ttgctaaagc tactgcttcg gcaacaggat aaactttact
     4621 tgaatcaact ttttttaatg catcttgcat ttttttgctc tttttagcca ttttggttcc
     4681 tccttgattg tggttataac ggttgtacct cccacgtgtt ctatatcttt caatatagag
     4741 ccaactgaga agctgctgtc ttagggtgta tagattattc tacagtgatc cccatgcttc
     4801 gtgcagttcc ttcaaccatg cgcattgctg cttcaacgtt agctgcgttt aggtcttcca
     4861 tttttaattc agcgatttct cttacttgat cgctagaaac tttcgcaact ttatttttgt
     4921 ttggttcgcc tgaacctttt tcgattttag ctgctttttt caatagaaca gcagctggcg
     4981 gtgtttttgt aacgaatgtg aatgaacggt cttcgtatac agaaataaca accggaatga
     5041 tcaaacctgc ttgatcagct gtacgagcgt tgaattcttt tgtgaatccc atgatattga
     5101 tacccgcttg acctagtgca ggacctactg gtggagctgg agttgcttta cctgcaggaa
     5161 tttgcaattt aacgattttt tctactttct ttgccacgag acatacctcc ttaagtccgt
     5221 gatgtggtta attggagatg catatttctc ctcccacgtt ttatctgatc tgaaaaaatc
     5281 cagacaaata atgtgcaaaa caatgcacac tggtacagta tagcaaattt atttgttttt
     5341 tcaagatttt tttctgctat ttttacattt tgcttgaata aacgctaaat ttattctaaa
     5401 aaaacaacaa tttttcgttc tcatttcttc ttttatgtta cacttgcgct agcatggttc
     5461 aacagatgaa agcaaacaat aacacggttt tttagaccct tgtacaaaga ttcctacata
     5521 acaaaatgag gtggagaaaa tgaaagattt aaggtataga agtgtctttg atattattgg
     5581 acctgtgatg attggtccaa gtagctctca cactgccgga gctgctagaa tcgggaaaat
     5641 catacgtagt atatttggcg aacagcctga ttctgtagat atttatctat atgaatcatt
     5701 tgcaaaaacc tatcgggggc acggaacaga tattgcatta gttggcggcc tgttagggat
     5761 ggaacccgat gatgaacgtt tagctgattc tttgaaattg gcatatgaag ccggtatgga
     5821 agtcttgttt gttcctaaga gtgaaaaagc cgaacatcca aactctgtaa agatgcttct
     5881 aaaaaaagga acacgaaagc tttcagtcac tggcatttcg atcggcggtg gcaatatcca
     5941 gatttctgaa ttgaatggat tcaaaatttc tttaagcatg ggtactccta ccttgatcgt
     6001 tgtccaccaa gatgttccag gtatgattgc taaagtgact agtatacttt ctgagaccaa
     6061 tacgaatatc ggcactatga cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat
     6121 aattgaagta gatgatcctc aagtagaaga aacggtaaaa agactcaaac agctgccaaa
     6181 tatcgacagc gtaaattatt tcgattaagg agctaaaaaa atgtttttat cgattgaaga
     6241 attagtagat caagcaaaag cttatccaag tgtagcagaa ttgatgattg cagttgaaat
     6301 ggaaatgagt ggccgttccc gtagtcacat catcgagatc atggaaaaaa acctctcagt
     6361 aatggaacaa tcaatcaacg agggaaaaga aggtgttacc tccgtcacag gaattaccgg
     6421 tggtgatgca aaaaaattag atgattattt aaatgaggca gactttttaa gtggagaaac
     6481 aatcttaaaa gctgtacgca atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt
     6541 gatttgtgct acaccgactg ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt
     6601 ccgagatcgg cttgatttga cacatgaaca gcaattggaa ttcttattta ccgctggcgc
     6661 tttcgggtta gtcatcgcaa acaacgcttc tatcagtggt gcagaagggg gatgtcaagc
     6721 agaagttggt tctgcaagtg caatggcagc agcagctttg gtttgtgcaa aaaatgggac
     6781 tcctcatcaa gcagcacaag cagtagccat tacattgaaa aatatgatgg gactgatttg
     6841 cgatcccgtt gcaggcttag ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc
     6901 tcaagctttt atctctgctg atatggcatt agcgggtatc gaaagtgtca ttccgccgga
     6961 tgaagtcgtt gctgcaatgt accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac
     7021 agcagaaggt ggcttagctg ttacgccaac tgctaaacga cttgcggaag agattttaaa
     7081 taagagggcg tgataaagct ccttaatttc gagtattaag agattagtta tcttcataac
     7141 aaaaaacgaa gagaggacta attgcccttc tcttcgtttt ttattaatcc atcaatttcg
     7201 tattttttac ttcatcgatc gtttttgttc ctgcaagttg catcgtgatc gacaattctt
     7261 tgttcaggtg ttcaaaaaca gatgttactc cttctgcccc acctagattc aacccataaa
     7321 tcactggtcg gccaatcgca actaagtcag caccacttgc cagagctttg aagacatgct
     7381 ctcctcgacg gacaccagag tcgaaaatca ctggtacacg tttgttgact acttcggcaa
     7441 tttttggcaa tacttcaaag gacgcaggac ctccatctag ttgacgtcca ccatgattgg
     7501 atacccagat accatctgcc cctgcagaga tagcgattac tgcatcttct ggcgattgta
     7561 ttccttttac gataactgga agatgggtga tttctttgat tgttttgatg tcatctggtg
     7621 tcaagccttg ttttgcagct gcataaattt ctgcgattcc ttttccttcg ccattaccac
     7681 ttgcactttg ttcgctatac gcagctaagt taggcattgg taatggaaat tggaattgat
     7741 tgatgacgtc ttcttcacgg taaccaccga gcgttgaatc tgccgttaaa atgatagctt
     7801 tagcccctgc ttctactgct ttgttcaaaa tgaactcatt gaatctgtcg tctttactca
     7861 tatataattg gaaaaactgc ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg
     7921 tatttgcata agtgctgatg gaaaagattg atccagcagc agctactcct ttagctgtat
     7981 cagcttcccc tttttcatgt gccaatcctt gggccgctga tggcgcttgg ataatcggtg
     8041 tatctaattc aattccgaaa acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata
     8101 tccgtggcat gattttcttt gtattaaaag aagtcgtatt ttctttcatc gtccactcat
     8161 cttcagaacc accgcgaata tagccaaaag ctcctgcttc catgcgctct tttacacgac
     8221 cttctagtta ggcaatgttt acaatatcga ttggatgttc ttcattactt gtatgataag
     8281 tcatagtaga tccttctttc atcttcttga ttacatataa atcatactaa acttacaaaa
     8341 agtaagcaaa catttactct cagatacttt tatcgctatt ctttccaaat aaatcgactt
     8401 acaagctgat ctacttcttg gttttcatga agcatactat gagaggcatt ttcacctgtc
     8461 actacttttt cctctatttc atttccgcgt tgcttcaaca acgcatagac cgctaatgca
     8521 ctgtttaacg gtactgttcc atcactcaag tctgtttcat ctagttgacc agcaatcaag
     8581 aaaaatcgag tggttatagg aatattaccg atcatttgct ggtaatcttg atagcggtta
     8641 gaaacgacag caggcccctt tgctaactca tctgtcagag actgcgcact atcatcgaca
     8701 aaatcattaa aaggcgcgcc aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc
     8761 tgtccatacg ttcctaaata gcgtagtgca ctaactcctc ccatggaatg ccccactatg
     8821 ttgactttat ctatgccgta gttttctttc agatacaaaa gacaagtttt gatccattct
     8881 gcttgattcc actcgttatt tttattatct tcaaacaata cttggaccat cggattgtct
     8941 gcttttccag acaaatctcc tgttgcttgt atttgtccat ctgttcctac tgttaaaacc
     9001 aattctttta ctgcttgctg atttttttcc aaacgccgca acatcccttt aaaggaattt
     9061 tctgtcccgc tgtagccgtg gaaaaaaagt gttggaatag tggttttttc tatcacttta
     9121 ctggattctg ccgttttttc tttatcagaa acttcagtag tatttatctt cgtcgtctgg
     9181 caaccactta acgcaaacaa actgataagg actaaagctg ctgcagttgc acgtttgcat
     9241 aaatttttga ttggttctgt catacacctc ttcctttcca tatagaaaaa agaaagactt
     9301 gaaaggccaa tcatatgatc gacatttcaa gtctctttat taatcgattt tatctacttg
     9361 atcgaaatca agttctgtac tggtttcacg tccaaacata tcgatgttga ctttcaattt
     9421 ttctttttct ccgtcaactt ctgttacttg tccttctaat ccagcaaatg cgccttcgat
     9481 gattttcacg acttcgccaa cttctacttc tagatcattt tgtcgcaggc tcataccgat
     9541 agaacgcaag atatgattga cttcttctgg aagtaatggt gctggtttgc ttccggctcc
     9601 gtgtgaacca acgaatcctg tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc
     9661 tgtcatgatc atttcaacaa gtacgtatcc tgggaatgtt ttatgaacga tttctttttc
     9721 tttcccattt ttgacttcag tttctttttc ttcaggaaca acgacacgga aaataaagtc
     9781 ggccattttc atactttgcg cacgagattc gatatttgct tttactttgt tttcatatcc
     9841 tgaatatgta tgcaagacat accagttttt ttcaaaagat tccattgaag gctcctcatt
     9901 tcattgtaaa ataaaaaaac ctcagtacac ccgaagtttt tctttcgctt cattatagca
     9961 ctaaaagtac gttgtggcta gcgagttatt ttaaaatcca gccaaataag ctttggatgg
    10021 ctgtatccat tacgtaaaac atcaatccga aaataataga agtttcgatg actactaacg
    10081 tatctttacg gagttgtttt ctgcttggcc aagttacttg tttgatttca tcttttacgc
    10141 tttttagaaa tttcatgaga caacctccct tgctactgtt tatttcgttt ctttatgtaa
    10201 tgtatattga ttacaatatt tacaaaactt attgatttcc aaacgttcgc cgctttttcc
    10261 ttcactgact gattttgtgt agttgcgaga cccacaaacg gaacaagcga gtgctgcttt
    10321 tttctttgcc atttacttct gcccccttgc tgagaatcag acttatactt cataacagta
    10381 tcacgtaact ttttacttgt caatttgtga aataaactat ctattgtttt ggataaaatt
    10441 atgatatgat taatttgaaa ttaagctaaa atgagacaat tggaggtata gcgatgctat
    10501 taaaaacact tgtatataag aaaaaagatc tgacaaccgt tacagagact agtacattag
    10561 aagaagcatt aaaaatttta gaaacttccg gataccgttg tgtgccgatc ttagattctt
    10621 caggtaatat tttccgtgga aacatttaca aaatgcatat ctatcgtcat aaagcaaatg
    10681 gcggtgatat gacgcttcct gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc
    10741 tggatacttc tttcttcaaa gtattcttta cgatcaaaga gcttccttat attgctgtcc
    10801 ttgacagtga aaatcatttt tatggtattt tgacacatag tacgttgctt aatatgctct
    10861 ctcaatcttg gagcgtagaa caaggaagct atgtactgac aattgcttcc acaggaaagc
    10921 aaggagattt ggctgctatt gcaaaaatca tttcaaaaca cagcagcatt gccagctgta
    10981 ttactcttga tatcgaaaaa gacgaataca ttcgacgtac attgatcacg ctaccggcta
    11041 acactgaagc gaaagtatgt gaaacgatcg tcaaccactt agagaaaaag aattataaag
    11101 tggtagaaat tgaaaatttg caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac
    11161 agctaatctg ctttcttttt tcgcttttta taattagaca cgcttttcaa aatcaagcgc
    11221 gcgatctaaa taatacatca atggtgctga aacagccatg acgataaact cacttaatgc
    11281 aagcgtgcca taggttgtcc aaaatgcttg gccacctgtt ggaactaaca tataagcaat
    11341 cagtcccatg ctgatggtaa agaagatcgt gttcaatact aaacgcatct ttacattttt
    11401 cactttgttt tgcaataaag ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa
    11461 aaccacatcc atgataccaa aaccaaagaa ggcattatag acgatgaccc cgcctaaaac
    11521 tccccaaagc aatttacggt taaagacaac taaatgattc aaactttctg aaatacgaaa
    11581 ctggatcggc cctgaggata caggcgcaac taaaatagtt aacgctagat aaagagccat
    11641 gatgatgcca tttacaacga ttacggataa ccgttgtctg cttttcgttt tttgcatttt
    11701 ctctcctctt ttctcctagt ttttttaacg cgggatggtt gatgaaccgc ggttatgtga
    11761 aacaaacaat aattataaca gacttttcat ttatttcaat ccgtcttatc tgcttttaaa
    11821 aaagcaaaag cttaaacaat gtaaacgttt gctaaaaatc gtgggatatg gtataaagta
    11881 agtgtaaggg gtcataagaa ttcccagtaa agcagacgaa gtaggatctg caagaacaga
    11941 attgttgtga aacttaaata aataaaaaag gatgtgggaa tttgagcaac ctcttactag
    12001 agaacggtct agcaagcagc tgaagatcta agtggacatg ccgaaccttg gaacttctcc
    12061 ttacgtaagt aatggatcat gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc
    12121 tttttcattt tcctagactt ctgctataat aaccaagaat taattttgta acagattttt
    12181 ttgaaaaaac aagtttcttt cattcaaaaa atagaaaaag cttgctaatt attggttaac
    12241 cagcaaacag gagtattaaa atatcttgtt ttattgaata gcaggaggaa atcatgaaat
    12301 tccattatca tatattgatc aatccatcgg caggcagcgg aaatggctat aaagtagcag
    12361 aaagaatttt accggtatta aaaaataagc atattgacta tacacttcat tatagtgaat
    12421 acaaaggaca cgatgctgag attgctgaaa ctttggcaaa agaaacattg attccttgga
    12481 tagaagaaga acagaaaaca atcgatactt tccccctttt gatcatcgta ggaggtgacg
    12541 gcaccttaca tcaggtactt gatactttct atcagatgga agtagaattc ccagttgctt
    12601 atattccagc aggttctgga aatgattttg ctcgaggggc aggccttcca aaaaatccca
    12661 aaaaaggact tcagctgatt ttagcagctc aatcaccaga aaaagtccac ataatggctt
    12721 atgaagaaaa aataagtgaa aaaaaaggaa tagctgttaa taatttcggt ataggattag
    12781 atgcagctat cgtccatgcg acaaatcatt cttccacaaa aaagcgattg aacaaataca
    12841 acttaggatc attttcgtat ttattctcta ttctccgtgc tttattcact caaaaaggat
    12901 ttccaattct ggtagatgca ggaggaaaac aattaagttt ttcaaacgca tttttatgta
    12961 cagcaacgaa gcatcctttt tttggcggag gaatcgcgat tgttccaact gctgatgcgt
    13021 ctaaaccagt cattgatttt gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc
    13081 tcattctttt acttatacag aaaaaacaat tgaaatcgaa atatttccat cactacacaa
    13141 caaacagatt acgtatcgtt tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa
    13201 tggaaaaacg accttttgat atcacaataa ccacaactaa acagttgttt tggtgttaat
    13261 aaaagaggtt ttgtcagaag ctacatctgg aacaccgttt attcggaaaa aaggactgtg
    13321 acacgacttt tgtcacagtc ctttttttac tatgcagtta attctttgcc caattgggtt
    13381 gccatcgata gtttctttct ttacccaaaa taaattggat cattgaaaac accgcagcaa
    13441 aagcaactaa gacacaacca ttgaaccaaa aagattctgc aaatgtttga ttgtaactga
    13501 gtgtttcact gatcaagtta ttattttgaa caaaccaata tgttggtgtg aatgccgcta
    13561 ttttgctaac catttctggc aagtattctg ctggtacaaa caccccaccg ataaagcaac
    13621 tgcctgtgat aaaaatatct ttgatcccac cgctggtttc tgagtttttt accaatgaag
    13681 tgatacaagc cgaaaagcta atgatcggca ataaaaacaa gaaagtattt aaaatatgat
    13741 accataccgt tatatcccac gaggtgtgga tcactaacag aaccattaga agtaagaagc
    13801 cactgaacaa cagcagcgag taactaatca aactaaaggt tactttgcga ttcaagtttc
    13861 gtttggtaat gggcgcactc tggtttcgtt tactgatctc tttgcgatta aacgctagat
    13921 tgatgacgct gaaaccgcta aaaatggata gaaacaaccc ataagacaag aaattaaagg
    13981 ttgtgcccgt caacagcttt ttcatccgct gactatatcc cgcacttagc tcaacttctc
    14041 cagtaaccga gagtgccatt tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt
    14101 cttgaaacag aatcagtgtt tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag
    14161 aaaaggcatc tgggcgactc tgtatcttga tcgtcgccat ctttcctact tcgacttgtt
    14221 cggtaaagtc tgcaggcaga tgtaagataa tgtttacacg attaaaataa agagcatcat
    14281 cgatttcttt ttgtgaggtt ccatccaatg taacgactgt ttccttgtca ttcaaatatt
    14341 tacgaaggcc atcgactaca ggcgactctt cttcactaat caaggcgatt ttcgcatctt
    14401 gcagagaggt attttcaaca tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga
    14461 taattacacc tatcagtaaa ttcgatttat ttgctttaag caatgtccaa atcgttttat
    14521 aaagcgtcat aacgcacccc tctttccatc caagcatttc ctaacgcaaa aaggaaaata
    14581 aatccgatca accatagaag attggtataa aaactagcca aatcagtgta gaaataaagt
    14641 ttatacaaac tttcgctgat caagttgacg agatttaatt gaccaacaat cggaatatga
    14701 agatcaatcc aatacttcac ttgttccgac cccatcatac cagcaaaaaa gctcatcgca
    14761 atcgtcactg aaatcaagat gctatccttt tgcaccagat tcatttttgg tatcaaatta
    14821 ccaaccaatg tacctaacaa aatcgcacac aacgacccca gagcggtaac aatcatcaac
    14881 cattgccaac gttcaccaaa gtctacttga tagataaaac gaaagacagc taacataata
    14941 taaatctctg tcaaaacaat cgtaaagctt gctgccaaat tagcgattga aactaacgat
    15001 tgctttctag gaatcaagga caagcgaata ccatttgacg attgatttgc ttgctgatcc
    15061 tgtacattgc ggacccccca catcgtcccg tagagaatcg ccattccaat cagagtgaag
    15121 aaataaaaac ttttgaaact catattacga cctgcatgaa cttcttgaaa agatacttct
    15181 tgttcaaaaa cagtcatgat gggcgcgaga tcaacgttct ccacttctgc cactttactc
    15241 gaaattatac caatacgctg taaaaattga tccatgacgt ttttcatcaa cgtctgactc
    15301 attcctgctt gacccacatg gagcgaaaca gatgaaccat caatgagata aaaaccagcc
    15361 aaatcatctg catttagctt ttcattcgct tctacttccg atagttcctg tacttcaaat
    15421 aatggtttat cattgacttc gacttgttct aagactgtgg taaaattttc cgaaagcgct
    15481 gtttgatccg ctttgattac aattccagta ttaatcgttt caagattgcc accttgatca
    15541 atctcgccaa aggccgccat aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa
    15601 aacaacatgg atttatttcg cagcagtatt tttgtgcgat atttataaaa atgccaaaac
    15661 attatttccc ctcctctagt ctctcaaatc cttgccagta atctctaaaa agacatcatt
    15721 caaggacgct tctttggttg ctatttttcc gtaactgatc ttttcttctt gtaagattgt
    15781 aagaatcggc aacaatactg tatttagttc agtagttgtt acttggagct gacctttttt
    15841 ataagcgatt ccatcagaag ctattgttcg ctgaatcgct gacaacacat tttctgggag
    15901 taatgggatg tcgatcgtca aacgatccgc cgtccgtacc atatctttta gagattcttt
    15961 ggttccttta gcaatcacct gtccttgatc catgatcacg atctggtcgc aaagaatttc
    16021 tacttcttcc atgtaatgtg ttgtatagac aatggttgct ccttgtcgat tcagctcctt
    16081 gatactttct aaaattttat tccgactttg ggggtctaca gctactgtcg gctcatctaa
    16141 aaaaattaac cgcggcttgt gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc
    16201 accactaagt tctcctggac gaaacgaaac atatttttct aatcccacta attgaatcac
    16261 ctcttcaatc aaggctttcc ttttctcttt ttcggaaaca tataaaccgc aaaaataatc
    16321 gatattttct tgcacactta gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat
    16381 cattgccttg acagcataat tttcaggagt cattgttgtc ccgaacaatt taatggttcc
    16441 tttgtcataa tttaacaaag aaagcataca gttgatggca gtcgatttcc cactgccgtt
    16501 tggacctaac agacctaaga cttctccttc ttgtacttgc agatcaaaat gattcaatgc
    16561 tacaaaatct ccatagcgtt tgactaattt atcaatttcg ataatcatca tgaaaaactc
    16621 cttccaaatt tcttgttgat ctgactataa taaagtgcaa gaaaagaaac tagtgacgat
    16681 tggctaaacc acacatgaca tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc
    16741 tactcaacga aactagcttt ctactggtta tctcattgct tttgctggta agtcaaggat
    16801 tcgttgctat gcaagttatc tatttattga tcgccgtttt attcactggt gtcagttatt
    16861 tactgcctac aaagccaaat tggattttcc aagttattct atttctttta tgtgcctttt
    16921 tacctgatct tgtcctgttt ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc
    16981 accttgatta cttgtttttg ttaggttttt tgttgatttc gacggttggt aatcatcccc
    17041 ccatcaaggt cgcctttgtc gtaattttgc tgtttttttc cttttactta cgaaaaaaag
    17101 atcaacgaac gaccgatctt gagaaaaagt acattgtctc caaagatact cattgggaaa
    17161 aacaacaaga gttatcggaa caaaataaag cattggccct gtcacaagaa acacaattag
    17221 ccttgcaaat tgtggaagaa cggaaccgaa ttgcccgaga catccatgac aatgtcggtc
    17281 atctgctgtc aagtgcgatt ttacaaattg gtgcgcttga aatgatcaat caagaaccaa
    17341 aactaaaaga accattagaa aatttaagtg ccaccgttca cactgggatg gatcggattc
    17401 gtcaaagtgt ccatgacctc catgagacat cgatctcttt tcaacactcc ttagagtttc
    17461 tgatcgctga ttttccttct tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg
    17521 aaacgcagca aaactgcttc ttaatggtca tcaaagaagc cttaaccaac agtatcaaac
    17581 atagtcatgc caaaaccgtt gttttacatt ttcgaacgtt gccggccttt tatcgcttgc
    17641 aaatcacgaa tgatggcacc agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa
    17701 cgatgcgtca acgtgttcaa gaacaaaagg gacaagtcca tattttccaa gaaaaccagc
    17761 aatttcagtt aacgatcatt ttaccaaggg agggacacca atgataacag tagtcattat
    17821 tgatgatgat ccatttgtga cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat
    17881 ccgtgtgtta ggcattggtc attgtgccaa ggatgcgcga aacccatcgt cccgatgttt
    17941 tgctgacgga catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg
    18001 ctaaatttcc caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc
    18061 gcgaagcatt ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta
    18121 ttccttctgt caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga
    18181 ctttcactca aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta
    18241 gtgaacgtga gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg
    18301 cagatgcctt gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa
    18361 agctagattt acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag
    18421 tcttatctaa cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac
    18481 atcttctatt caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc
    18541 agggctgcca ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc
    18601 taacgttttt tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt
    18661 caatttgaat tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc
    18721 taatttcgct aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt
    18781 cagcttagca tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc
    18841 tatttctcat ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt
    18901 ttccatacca atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa
    18961 atctttagaa acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa
    19021 cagatctggt tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc
    19081 ttctacaggt agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa
    19141 tggatcattt ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt
    19201 cattttatca ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt
    19261 cgctacacaa gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg
    19321 agtcactgcc gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg
    19381 caaaataacc atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag
    19441 agtgatttta ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac
    19501 taattcatcc actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat
    19561 atctccattg aatacattca attcgggagt attgactaat tggaggactt tgtctcctat
    19621 ccgataaacg gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat
    19681 ctcctgcatc attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa
    19741 tacttggata tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat
    19801 caagggttca atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt
    19861 gaaatctttt ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc
    19921 atcaccttgc cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag
    19981 atcatggagt acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat
    20041 aacttgcata ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat
    20101 agacatctca tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt
    20161 tttttcccgt ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt
    20221 ttcgttcatt cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc
    20281 ttgggtatat tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat
    20341 tccgttgatc acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga
    20401 acggattgct tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt
    20461 ttcgatacgc cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg
    20521 actgagaagg cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc
    20581 gtaaagatta gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt
    20641 agcaacctga tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg
    20701 ctgaagcaat gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat
    20761 ctgatgcaag atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc
    20821 ggctagatta tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg
    20881 attttcttcg atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa
    20941 ctgactaccg aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag
    21001 ccgaatcgtc tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa
    21061 taccgaagga tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt
    21121 ttctgctgtt ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc
    21181 atttgcagtt gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt
    21241 aggatgatcc actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa
    21301 acttccggtg actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac
    21361 caaaagcact ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac
    21421 gaaataaggc atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt
    21481 ctggtgctgt ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca
    21541 ataatctgca aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca
    21601 tttcacgcaa ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag
    21661 ctccatgtcc tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa
    21721 cagtttcgat acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat
    21781 acagatctaa atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat
    21841 cgatcgactg accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa
    21901 tctctaccgg acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg
    21961 ctgtactgga ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga
    22021 tttcttcgta actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt
    22081 cctgattcca ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc
    22141 tgtctttctt ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt
    22201 gatgcgtcaa ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg
    22261 cagctgtatt cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac
    22321 acttttcttc gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata
    22381 aaaagaagtg ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt
    22441 ttgagatcaa atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt
    22501 ggtaagtaat atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat
    22561 cagttgaaaa acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac
    22621 gggcaatatt tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca
    22681 ggatcattcc tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa
    22741 aaaaaaacgt atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct
    22801 cactaacttt tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta
    22861 atataacacc ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt
    22921 tttctcttag ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta
    22981 tataagtctg ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga
    23041 tctttttgat tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat
    23101 ttcattgctt cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt
    23161 tgattcggat aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc
    23221 aacacttctc catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag
    23281 atatttaaaa ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca
    23341 caccaaaatg cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca
    23401 aatccaacag cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct
    23461 ccgcagccaa catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata
    23521 acgggctgat acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg
    23581 tattctgttt taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa
    23641 cggtgattat ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc
    23701 ggacagcgga acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt
    23761 aacatagttc cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc
    23821 taaaaatatg atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca
    23881 aagaattttg cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc
    23941 ggatcgaatt gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg
    24001 aagaaacgtt aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta
    24061 gagggggcga cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg
    24121 aagcaaagaa attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc
    24181 tagacgaaga ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc
    24241 atatggcttt tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag
    24301 aacatggtgt tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa
    24361 atttcccacg gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg
    24421 gtggtggtgt cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag
    24481 tacacggaac caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca
    24541 acaggcataa tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa
    24601 agaagcaata ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg
    24661 accgcttgct actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc
    24721 tgcaagattc gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat
    24781 aaaattatgt ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt
    24841 gacaacgccg gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc
    24901 agaaatcgca agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt
    24961 tcctgtaatc agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata
    25021 gtgactgatt aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa
    25081 acgtcgagac gctgccttta ctggattttc attaatttct tccacattga ccccggcaag
    25141 tcctgaataa tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac
    25201 gattcctaac aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct
    25261 aaatgtttgt gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct
    25321 catcagatca gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac
    25381 ccctactagc tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc
    25441 tgcttcgttc ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac
    25501 aaattcagtt gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat
    25561 ttcactggca tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag
    25621 cgtattaagc aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata
    25681 gctaggacgc atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc
    25741 gaacatatcc gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc
    25801 tgtaagagat tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct
    25861 gaatgcctga atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa
    25921 ttcttgattt tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact
    25981 gcctccatgt ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg
    26041 taaaaacgca gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg
    26101 ccatacatat catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga
    26161 tgtgtccttc ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct
    26221 atccagtact cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa
    26281 gaataatctc gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt
    26341 ccactgacta aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat
    26401 tttgcgtgcg caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt
    26461 cgcgtcacca catggattac ttggttttcg taaccttgtt ttttataata agcctttaca
    26521 cgattagcca ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt
    26581 ttatttacta ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa
    26641 agtacggttt catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct
    26701 agagaaaaca agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt
    26761 attttcgcta acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga
    26821 gaatgatctt tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc
    26881 tttcacacga tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc
    26941 tattcgataa taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac
    27001 aagataatct tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat
    27061 cggagaaccc aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa
    27121 ggcattgata ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc
    27181 agtaggcgta gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat
    27241 gtatacatcg gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa
    27301 aaaatgctgg tccggtgttt catccaaata actgatccga acatccagca agggataact
    27361 aacactttgt tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg
    27421 ccaatccgtg taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag
    27481 acaatgactg tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac
    27541 caattctggc tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg
    27601 ttctgtcact tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg
    27661 cctcctctct ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc
    27721 ctcttccatg ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct
    27781 cttcatccaa ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg
    27841 gaaaaacgcc ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg
    27901 caagagtccg aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag
    27961 gatactcgat caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat
    28021 ctcgttcttg gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga
    28081 tatcttctac aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta
    28141 aacgtcccat tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga
    28201 ctctcccagt gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga
    28261 tcccccgcag tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt
    28321 cccaatctcg ttccatagct gatacctcta cttcattaac attcattgtt tattctacca
    28381 tatatgcaaa gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc
    28441 aaaatagaca aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta
    28501 tgctgacaaa gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt
    28561 tccgtgtaca gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt
    28621 gtggcctacg gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg
    28681 aaaaagggcg tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct
    28741 tcgaaaaacg tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg
    28801 atcctgcttc attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg
    28861 aagaaggact tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg
    28921 aactagaggt aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt
    28981 tttctttacc ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt
    29041 agaaaccttt cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt
    29101 ataggatgtg aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata
    29161 tgatcgaaat ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac
    29221 agttattaca atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc
    29281 gctttcttcc tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt
    29341 ctcagaacaa tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg
    29401 actgcaaagc ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc
    29461 aagaagcagt cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg
    29521 aagaaatcgg cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag
    29581 aagtattctt ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg
    29641 cagtcgttta caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta
    29701 caagtagcct ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt
    29761 ttaatggtga acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac
    29821 acttgctttc taattaa
//
LOCUS       DACCYH010000029        29096 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.029, whole genome shotgun sequence.
ACCESSION   DACCYH010000029 DACCYH010000000
VERSION     DACCYH010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.029"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            278..1063
                     /locus_tag="IVY85_001228"
     CDS             278..1063
                     /locus_tag="IVY85_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8044255.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1076..1927
                     /locus_tag="IVY85_001229"
     CDS             1076..1927
                     /locus_tag="IVY85_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP8044256.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(2051..2503)
                     /locus_tag="IVY85_001230"
     CDS             complement(2051..2503)
                     /locus_tag="IVY85_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8044257.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(2968..3336)
                     /locus_tag="IVY85_001231"
     CDS             complement(2968..3336)
                     /locus_tag="IVY85_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP8044258.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            3486..3968
                     /locus_tag="IVY85_001232"
     CDS             3486..3968
                     /locus_tag="IVY85_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP8044259.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            4005..5015
                     /locus_tag="IVY85_001233"
     CDS             4005..5015
                     /locus_tag="IVY85_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP8044260.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(5130..5843)
                     /locus_tag="IVY85_001234"
     CDS             complement(5130..5843)
                     /locus_tag="IVY85_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044261.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(6033..6623)
                     /locus_tag="IVY85_001235"
     CDS             complement(6033..6623)
                     /locus_tag="IVY85_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP8044262.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            7107..7334
                     /locus_tag="IVY85_001236"
     CDS             7107..7334
                     /locus_tag="IVY85_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP8044263.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            7350..8516
                     /locus_tag="IVY85_001237"
     CDS             7350..8516
                     /locus_tag="IVY85_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP8044264.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            8750..9793
                     /gene="hrcA"
                     /locus_tag="IVY85_001238"
     CDS             8750..9793
                     /gene="hrcA"
                     /locus_tag="IVY85_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP8044265.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            9887..10450
                     /gene="grpE"
                     /locus_tag="IVY85_001239"
     CDS             9887..10450
                     /gene="grpE"
                     /locus_tag="IVY85_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP8044266.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            10501..12330
                     /gene="dnaK"
                     /locus_tag="IVY85_001240"
     CDS             10501..12330
                     /gene="dnaK"
                     /locus_tag="IVY85_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP8044267.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            12481..13647
                     /gene="dnaJ"
                     /locus_tag="IVY85_001241"
     CDS             12481..13647
                     /gene="dnaJ"
                     /locus_tag="IVY85_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP8044268.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(13729..14394)
                     /locus_tag="IVY85_001242"
     CDS             complement(13729..14394)
                     /locus_tag="IVY85_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP8044269.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            14370..14543
                     /locus_tag="IVY85_001243"
     CDS             14370..14543
                     /locus_tag="IVY85_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8044270.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(14588..15325)
                     /locus_tag="IVY85_001244"
     CDS             complement(14588..15325)
                     /locus_tag="IVY85_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8044271.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(15327..16829)
                     /locus_tag="IVY85_001245"
     CDS             complement(15327..16829)
                     /locus_tag="IVY85_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8044272.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            17088..19082
                     /gene="uvrB"
                     /locus_tag="IVY85_001246"
     CDS             17088..19082
                     /gene="uvrB"
                     /locus_tag="IVY85_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP8044273.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            19092..21908
                     /gene="uvrA"
                     /locus_tag="IVY85_001247"
     CDS             19092..21908
                     /gene="uvrA"
                     /locus_tag="IVY85_001247"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP8044274.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            22171..23436
                     /locus_tag="IVY85_001248"
     CDS             22171..23436
                     /locus_tag="IVY85_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP8044275.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            23440..24171
                     /locus_tag="IVY85_001249"
     CDS             23440..24171
                     /locus_tag="IVY85_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP8044276.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            24306..25190
                     /gene="rapZ"
                     /locus_tag="IVY85_001250"
     CDS             24306..25190
                     /gene="rapZ"
                     /locus_tag="IVY85_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP8044277.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            25187..26185
                     /locus_tag="IVY85_001251"
     CDS             25187..26185
                     /locus_tag="IVY85_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP8044278.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            26219..27154
                     /gene="whiA"
                     /locus_tag="IVY85_001252"
     CDS             26219..27154
                     /gene="whiA"
                     /locus_tag="IVY85_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP8044279.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            27697..28347
                     /locus_tag="IVY85_001253"
     CDS             27697..28347
                     /locus_tag="IVY85_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044280.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            28534..>29096
                     /locus_tag="IVY85_001254"
     CDS             28534..>29096
                     /locus_tag="IVY85_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAP8044281.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT         9565 a         4897 c         6176 g         8458 t
ORIGIN      
        1 aaggggctgt gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa
       61 gtagcttctt cggaaataag ccgaaatttc ttgaagctga ccacttctgt cacaacctct
      121 ttctttatag aaataaaagg acactagcaa ttcatgaaaa cgattgctag agcgtatcat
      181 ttgctttaga ctatatatct tggtgtacac tcactttaag gcggaaataa aagttatccg
      241 taataacagg tttaggtatg aaaaaggagt gggtactatg tacaaggaat tagaaaacaa
      301 agtcgcagta gtaactggtg gctcaaaggg gattggaaca gcgattgctc aacgattcgg
      361 gaaagaaaaa atgaatgtag ttgtcaacta taactcggat aaagaaggag cagaaaaagc
      421 agtcaaagca atcgaagagg caggcggaaa agcagtctct gtacaagcag atgtcagttc
      481 tgaagaagga atacagcgtt tacttgatac ggctttagaa aattttggga cactggacgt
      541 ttgggttaat aatgctggga tggaaaatca gcatccaacc catgaactta gccttgaaga
      601 ttgggaaaga gtgttaaaag tcaatttgac tggcgttttt atcggaacga aagcggcttt
      661 aaattacttt acagagaata ataaaaaagg aaatatcatc aatatgtctt ctgttcatga
      721 acagatccca tggccaacgt ttgctcatta cgctgcttca aaaggcgggg taaaattatt
      781 taccgaaacg gtagcaatgg agtatgcacc gaagggaatc agaatcaata gtataggccc
      841 tggtgctatc cgtacaccta ttaatgcggc aaaatttgat gatcctgaac aaaaagaact
      901 cttggaaagt atgatcccac taggacgcgt aggtgaacca aaagaaatag cggcgacagc
      961 tgcatggttg gcttcagacg aatccagtta tgttacaggt attacactat ttgcagatgg
     1021 tgggatgact ttatatcctt ctttccaagg tggaaaagga taagggtgaa taaaaatggg
     1081 tttattgatt gcattgatcc cggcagttgg ctgggggatc cagccactga ttttgaaaaa
     1141 gattggtggc agaccaacaa atgagatatt aggaactggt attggagcgg tgcttgtcgg
     1201 cttactcgtt caattattcg cttctccagc tggtatttca gtgactacat ttttgattag
     1261 tttactttct ggtgcatttt gggttattgg acaaacagga cagtatacat ctttcaactt
     1321 gattggtgtt tctaaaacga tgccaatttc gactgcatta cagttagtcg gaacctcgtt
     1381 gattggtgtc ttcgcttttg gagaatggtc tggaagtatg gggaaaatca ttggagcagc
     1441 agcaatcatt cttcttgtat ttggttctgc tttaacggca atcagtgatg atcctggaaa
     1501 aaaaggcggt atcacaaaag gaattagtct attagcatca acaagtattg gttattgggt
     1561 atacagtgca ttgcctaaac tagttgatgc aagcggactt tccattttct ttccgcaaat
     1621 gatcggtgtc tttttagggg cagtgattta tgtgattttc cgtaaaccaa ccgcattttc
     1681 tgaagacaag agttggaaag caacgatcgt tggagccatc tttagtgtgt cagcattggc
     1741 ttatattttt tcagcaaatg aaaatggggt tgctactgca tatatcatta cacagctaaa
     1801 cgtagttatc tctacattag gcggtatgta catcctgcat gaaaataaga caccaaaaga
     1861 aaaaaaatta acgattatcg gattattgct gatcgtagta ggaagcatgg tcacggtctt
     1921 tatttgagga taaagatggt aaaatttcat ttattaaaaa aatatttgtt tgaaccaaac
     1981 agtaagtact tataaaaaaa tagcgctaga atttccaaca tctctggaat ttctagcgct
     2041 ttttaattgt ttaagataca agggaaatat ctgtctggat caataatttt tcattgatcc
     2101 attgactaaa gtttacgaca taattgaagt taccaccgat tagtatctcc gcatgattgt
     2161 ctttataaag atcgtattgt aaaagacggt cttttttatt tgcgtaaaca tcgttatggt
     2221 actggactaa ttgttctcgg aattgattga ttgctgtaaa atcacgatat tgaaggttga
     2281 aacgcatcgt gattggtgca tttagcatga atcgctgcat ttcttttgct gcttcctctg
     2341 cttgatctcc gtataggaac tcaaaaagat tacgttcaga aaatttccat tgcccgccaa
     2401 cctttgttcc ttttagtttt ccttcatgaa gataatttct gatagaacgt gtagtaacac
     2461 ctaattgttc ggctaattca tttactgagt aaaaatcttt caatgttcac cctcctatgt
     2521 gaatggttct actgacttag ttgatatttg cttaatcacg aaatgtagaa agaacgattt
     2581 taattaaggt cattgtaaaa atcaagtcag aaaattctaa cttttatttc tctaaagaaa
     2641 ttcgttctct cctaaagtga tattatattt aaatatacct gaaaataatg ggcagtatta
     2701 tgaaagatca ctgatatttt ctcgtatttt ttccttattc aaaagttttg caggaaaatt
     2761 gctgaaaaaa taattttact aagtagaaaa ttcaaagcca ttcaatcagt gatttttttc
     2821 agcacaagaa agactcctta taaaaatagc agatactttg tcaattaagt gtttaaccag
     2881 ggatgataaa aaggatagga ttgaacaaaa cctgtataag gtgatgaata gtttcctcgc
     2941 cagtaaaagg tttattcgta taaagcttta tcgccaaaat ttatcatgac cttttttatc
     3001 ttcaatatta aatgaaatca attgttcaat caattcccag ttgattacac ttttccaagg
     3061 aactttgatg acgttatccg tatatacgta gcctgcttct tggatatttt tttcaagggc
     3121 acgaatcgct gctttttccg gtgcgataga aaaatgattt ttagcaaaac taaatgcaat
     3181 aataaaggta ccatggtcag taaacatagg ttggttccat tttacagttg tgtctagctg
     3241 gggatacgtt tctgttatct tagcaaaaag gtctttgatc ttttgttgat gttctttttc
     3301 tttgatttga ttgatgatcg gctgaaattt ttccattcta aaacgcctcc attaagtggt
     3361 ttgatttatt ttaccagaaa aacaatagtt atgtataaaa aacgaacaaa acacaagtaa
     3421 tgttgtaacc gctatattta tatgataaga tgtctcaaat aataaaatag aaaagagaca
     3481 taaaaatgat tgacttggca aagaaattct tacacgaaaa tatatatttt gagttgtatc
     3541 atcatcgagc catttataca aatgaagatg ctttggtaat aaagaaagaa caaggattta
     3601 caggtacaga gaccaaaagc ttattcttaa aagataaaaa agataataac tacgtatatc
     3661 tgacttttac tacgaaacaa acggatttca aagcattagg gaagatcgtc ggtaaaaggc
     3721 tttctattgt ttcatcagaa cgcatggaag aacaaacggg acaaaagcag ggcgctgttt
     3781 ctccgtttgg ttatgaaaaa cagattccga ttattattga tgaagagctc ttatcaaatg
     3841 aaaaacttgt ttttgcacca ggtagaccag atcaaacgat ggtcatccaa acggttgact
     3901 taaacaaaat catccagcta cttgagatgc cgatattttt gatgccggca aacgaaaaaa
     3961 acaattaacc aagtaaaata gtgcatactt ggaggtgaag caacatgata agttatccat
     4021 tattagaaag aaatgcaacg attggggtta ctgcaccatc ctcaggcgta tcagaggaac
     4081 accattcgtt actcgaatca gctttaaaga gaatggaaga aaaatctttc caagtagtta
     4141 ctggaaagac gctttggacg caagataagg aaaaatcagg tccagcaaga aaacgagcag
     4201 atgaattcaa tcaaatgatg caagatgaaa atatccaaat gatctttccg acttggggag
     4261 gagaattatt gattgagatt ctcgattttg tcgattttga tgctctacaa ccaaaatgga
     4321 tagtaggtta ttcagatatt agtacgttgc tacttgctgt cacattaaaa acagggatag
     4381 ccactgcaca tggaactaac cttattgatt taagaggaga gtacttggat gaaacgacag
     4441 caatgtggga aagtgtactg aaaacaagag aaggagaatc gattctacag caatcttctg
     4501 aaaagtatca aaaagtgtgg ccccatgagc attcatcatc ttctgttttc catctcactg
     4561 aaccaacaaa ttggcaatca gtcagcggag atgcagtaag gatgaaggga cgattattag
     4621 gtggttgtat tgatacgatc aggcatttaa tcggtactcc ttatggagat gtccaatctt
     4681 ttaaaagaaa gtataatgat ggaaagccga tcttgtggtt tctagaaaac tgtgagctat
     4741 cgtcagttga tttaagaaga tcacttgttc agatgaaact ggcaggctgg tttgatggca
     4801 gctcggggat catgttcggt agaagtgcgg cagaggattt gttagattat aaaatgaagg
     4861 atgtatacga agatctgtca aaggaattaa aactaccgat cttgtatgat attgattgtg
     4921 gtcatgtacc gccacaactg acattgatta atggtgccta tgcagaagta gcggtagaag
     4981 atagaaaagc gactgttttg caaacgttta tttaaaaaat tttttcctgc cttggagtct
     5041 atcaaagacc aataaatcta aatagaaaag aaagtgaaag aagctgacaa aatgctgtca
     5101 gcttcttttt gtataacata ttagaaagtc taattatcta taactgtcac atcgattaac
     5161 tgtttcacgt cattgggatt tcctgtagga atagctacta cagatgcatt atcaatgatt
     5221 tcttgagttg tttctctaat gaacgctgga taagatgttt gatctgcagg catttgccaa
     5281 tgtgaaggaa ctggataact agtgattgtc ccgtcatggt tacttgaata cactaaaact
     5341 ccctggtaac tgtatttccc agtgagcata accacatctt tcggatagac tgcactccct
     5401 gcatcataag gggcgatagg tgttcctgca ggataatgaa taacatgtaa ttcgaaatca
     5461 cccgatgtat ctctaaaagt attacctcta atagctagcc aaacacgtgc gtactcaatt
     5521 tgtagatcag aatatcctgc taacggatct attgactgag aagactgtga ggaggttgtg
     5581 tctattgttg aagaagaaat atttgtctca actgtactac tagatgtaga agaagtattg
     5641 ggctctgaga ctttactgct tgttgaagat gtattcgttt cagtagaagc tgatttctga
     5701 gtacttgatt tttcagctga actggtttgt ctgattgaac ttttgtctgt ggtcgcagat
     5761 ggatcactat tttctttact aggagacgta catcctacaa ggaataaaga gaccaacaat
     5821 aaaataaaac ctaacttttt catataaaat acctcattat ttttttaata ccagtatagc
     5881 ataatcctga atttttgaat ccgtaaaacc ttgtctatga atataaatag agtgtatctt
     5941 ctttgataaa tgtcagaaaa agtgtgacaa ataatcatca gtaaaattac ttgtcacact
     6001 attgataagg agtatttaat tgaagagaat gattaggaga aaggatctcc gtacttttta
     6061 tagtaatcat agaatccaag tttgtataga cgaatcgttt tttctcttat ttcttctgtt
     6121 tcaaaatgga ctgcttgaag aatcaagtct gtgaattcta taggagctgt tccattcgca
     6181 gtaatcaaat tacgatcttt cacagcttgt tgttctataa aatcttcttt attctgataa
     6241 ttttccgatt ctaaccaagg aaattgcgca tttccggtgt gtttgtaacc ttgcaataat
     6301 ccgctctgag ctaaataatc aactgcccca caaatagcag ctactggtac gtctgttttt
     6361 aacaatttat cgattatttg atataaatga tgatttttta tgtcccatga attgccaccg
     6421 atcataataa aaaggtcaac tttttcaggt atttgttcga ttaagtaatc gatttttgtt
     6481 tttaatcctc caatggaagt gacctctgat tggatagaag cagttttgtt ttgccatttc
     6541 tcattttggt ttagtgtgct agtaatataa gcaccttccc aatcggcata ttcctctaat
     6601 agaaacgtaa tcgcagtttt cattttcaag ctctccttta ctatctataa caaggacaac
     6661 agttttaacc tacagacatt cattaaaatt gggtagcgac agcgaaaaca acaattctta
     6721 agttttagac agagtataca gatagtcaac ttaattaaat acgaactaaa taaaaatgac
     6781 aggtaatcat ttttgtttac cagcattata acattttgca tacatggatg acaaaagaac
     6841 caattcccac ttgcttgagt acaatattaa aagaaactgg aaggcaaaaa gtaattatag
     6901 aaacgaaaaa taaatcatat cgggcactca gacatgaaaa caaccctaga gatatataac
     6961 taggtaacca gtttaatgaa agaaaaaatc atccaagaag tcgattcttg gattttttag
     7021 tggatttgtt cgccaaatac gtttagtatg cgtatatgag aggttgtaag caaagcgttg
     7081 tggtctgagc acttaaggag gaatctgtga aaatagcttt tcatattttt gcagattttg
     7141 acttaatgcc gaaggtcagc tttgcgatta ccgtttatcg agaggctgta aaagcaccgt
     7201 gtagctccga tgacaataag caagcatttt tagagctagg tttccttgat tctaaatatg
     7261 cggcttattt caatgagttg gtgcttgaac gcgattttaa gaaggtcgaa aacaggcacg
     7321 acttttcgac gtaaggagaa agaatttcaa tgatagaaac aataaatccc gcagaaaaca
     7381 aggtttctgc gtacattcat atcccatttt gcgagcatat ctgctattac tgtgatttta
     7441 ataaagtttt tttagaaggg cagccagtag atgagtatgt ggaaatgctg ctgaatgaaa
     7501 tgaaaataat gctagaaagt catccgacag cgaatttaga aacactttac gtagggggcg
     7561 ggacaccaac ttctttatct gctaaacaac ttgatcgact gttatcaggt gctagagaga
     7621 tcctgccttt tcaaaatggt aatgagttta ctgtagaagc gaatccaggt gatttgaccc
     7681 gtgaaaaact tcaagtaatg aaaaactatg gcgtcaatcg tttatccatg ggagtgcaga
     7741 catttgataa tcgcttattg aaaaaaattg gacgtaaaca caccgcagaa gatgtgtatc
     7801 aaactatgca atttttagaa gcagaaaatt ttaccaatgt aagcatcgat ttgatttatg
     7861 cattaccggg acaaacacta gaaggatatc tggatacttt ggaaaaagct ttggctttgg
     7921 atctgcctca ctattctttg tactcattga tcctcgaaaa caaaacaatg ttcatgaact
     7981 gggtacgaca aggaagactc gaactgcctg atcaggaaac ggaaaccaga atgtttgaag
     8041 agacgattga agcaatggaa aagcatggac gtcaccaata tgaaataagt aactttgggc
     8101 tctccggaca tgaatctcaa cataacctca tgtactggaa caatgcccac tattttgggt
     8161 ttggagctgg tgctagcggt tatcttggag atattcgtta ccgcaacaaa ggaccaatcc
     8221 aacactattt acgcccatta cgagcaaatg aacgcccggt attggaagaa gaaaaattga
     8281 cacgaaaaaa tcaaatagaa gaagaaatgt tcttaggact aagaaaaaaa acagggatta
     8341 ctaaaaaaca tttctatgat cgctatcaaa aaacaattga atcggtgtac ggtgatgtgc
     8401 taagggaatt agagcaagaa gggttacttg ttaatgaaga agaccgtgtt tatttgacac
     8461 caagagggac ctttttgggg aacaatgtgt ttgagcgctt tttgtttgaa aaatagttta
     8521 aagaatattt gttttttatc tgaatttaaa taaaaaaagt tcaaaaatac aataaaggac
     8581 tattgaaacg ttgatataac tggatttaca tcaattattt agaattagca ctcttgtgtt
     8641 tagagtgcta aaaaatggag tctttctctt gacaggagaa gggcttcttg ctatatttat
     8701 aagtggatta gcacttagaa tatatgagtg ctaatgagag gtgaggaaga tgttaacaaa
     8761 gcgacaaaac gacattttac gtctgatcat ccaacactat acgagtagtg gcgtgcccgt
     8821 tggttccaaa acattgatgt ctgagggagt caaggccagc tcagcaacga ttcgcaatga
     8881 tatgaaagca ttagaagatg aaggattgct gttgaagact cactcttctt ctgggcggat
     8941 tccatctgct ttaggctatc gttattacgt ggatcatttg ttaaaacctg ctcgcgtagc
     9001 agaagatgat ttgctgctga ttcgtcagtc tctaggcaga gaatttcatg aaatcaatga
     9061 gattattaag caatctgctg agatattatc tgagctgaca agctatacga ctttttcatt
     9121 gggacctgaa ataaaagacc gccgactgac tggctttcgt atcgtaccct tgaataatcg
     9181 acagattatt gctatcgtcg taacagatat ggggaatgta gaaagccaag tgttcactct
     9241 tcccgaatat atgggaagtc aggaactaga aaaaatggta cagattgtaa acgaccgtct
     9301 agtcggagat cctctgatga cggtgtacca taaacttcgt acggagattc cgatgattct
     9361 tcataaatat ttccaaacga cagaagggat gtctcagtta tttgacaccg ttcttggtca
     9421 tgtttttgaa gataaggttt atgtcagtgg acaaatgaat ctcttggatt atgagccccg
     9481 tcaagatctt gatcagttca aatcgatgtt ttcttttatg aaagactcag acggacttac
     9541 acaaatgatc actccgatgg atagcaatat tcatatcaag attggtgctg aattaggcaa
     9601 tgatttattg cagaatatga gtatgattca agcaagttac gagatagatg gtcatggaag
     9661 aggtacaata gccttgttag ggcctactag tatgccttat tctcggatgt tcggtctagt
     9721 cgatgtttat cgaagagaac tagcagctaa acttgctgat tactaccgtt cgcttgactt
     9781 gtcaaactcg taatgtgaat aacgaagcta tttgcagctg atcgttctct ctttcttagt
     9841 aagagcaatc aaacagctga ctgaataatg aaaggaagac tgtatcgtga aagaaaatca
     9901 agaagaacta gataaagaaa tgacagatgc tcagccagaa ccggaaatcg atgtcgaagc
     9961 tgcagaagat tcgggtattt cagaagctga agcggaagaa ttcgaaacag cgaaattaaa
    10021 agctgagtta gaagaaatgg aagataaata cttgcgggct cgtgctgaaa tcgcaaatat
    10081 ggcaaatcga ggaaaaaatg agcgtgagca attacaaaaa taccgttcac aagatttagc
    10141 caaaaaatta cttccatcaa ttgacaacct ggaacgtgca ttagccacag aagtgtcaga
    10201 tgatcaaggg gcaggtttga aaaaaggcgt ggaaatggtc ttggaaagtt tgcgtaacgc
    10261 actggaagaa gaaggaattg aaaaaatccc tgctaaagga gaagcctttg atcctaatct
    10321 tcatcaagca gtacaaactg ttccagcgac tgaagataca ccagccgata cgattgtaga
    10381 agtactacaa gaaggctaca aattacatga tcgcgtgtta agaccaacca tggtcattgt
    10441 tgcacaataa ttgtgcggat ataaaactta cactttcaat taaagaagga gacgttaatt
    10501 atgagtaaaa ttatcggtat tgacttaggt acgactaact ctgcagtagc agtattggaa
    10561 ggaaacgaag caaaaatcat cgctaaccca gaaggaaacc gtacaacacc ttctgttgta
    10621 tcattcaaaa atggagaaat ccaagtaggg gaagttgcaa aacgtcaagc tgtgacaaac
    10681 ccaaatacga tttcatcaat caaacgccat atcggtgaag caggttacaa agtagatgta
    10741 gaaggtaaat catatacacc acaagaaatc tcagcaatga tccttcaata tatcaaagga
    10801 ttcgcagaag actatttagg tgaaaaagtc gaaaaagcag ttatcactgt gccagcttac
    10861 tttaatgatg cacaacgtca agcaacaaaa gatgctggta agatcgctgg tctagaagta
    10921 gaacgtatcg tcaatgaacc aactgcagct gctcttgcct atggtttaga taaaacagat
    10981 cgcgacgaaa aaatcttagt attcgacctt ggtggcggta cttttgacgt atctatcctt
    11041 gaactaggag acggtgtctt tgatgtattg tcaacagctg gcgacaacca tctaggtggg
    11101 gacgactttg ataacaaaat catcgactac atggtagcag aattcaaaaa agaaaacagc
    11161 attgacttgt ctcaagacaa aatggcttta caacgcttga aagacgctgc tgaaaaagcg
    11221 aaaaaagatt tatctggcgt atcaagcaca caaatcagct tgccatttat cacagcagga
    11281 gaatctggac cattgcattt ggaaatgaca ttgacacgtg caaaatttga tgaattaaca
    11341 gctgacttag tagaacgtac aaaagtacca gtacgtcaag cgctaaaaga tgcaggttta
    11401 tcacaatcag aaatcgacga agttatttta gtcggtggat ctactcgtat ccctgcagtt
    11461 gtagaagcag tacgtaaaga aacaggaaaa gaaccaaaca aatcagttaa ccctgatgaa
    11521 gtagttgcta tgggtgctgc tatccaaggt ggcgtgatca ctggtgatgt gaaagatgtt
    11581 gttttacttg acgttacacc attgtcatta ggtatcgaaa caatgggtgg agtgttcact
    11641 aaattgattg accgcaatac aacgattcca acaagtaaat cacaagtatt ctcaactgcg
    11701 gcagataatc aaccagcagt agacatccat gtattacaag gtgaacgccc aatggctgct
    11761 gacaacaaaa ctctaggaag attccaattg acagatattc cagcagctcc tcgtggtatt
    11821 ccacaaatcg aagtaacatt cgatattgat aaaaacggga ttgtaaatgt atctgcgaaa
    11881 gatctaggta cacaaaaaga acaaaaaatc acgatcaaat cttcttcagg tctaacagac
    11941 gaagaaatcg aacgtatggt aaaagatgca gaagcaaatg cagaagcaga taaagctcgt
    12001 aaagaggaag ttgacctacg taatgatgta gatgcattgc tattctctgt ggacaaaaca
    12061 ttgaaagaat tagaaggcaa agtggacgaa gaagaagtga aaaaagcaga aacagctcgc
    12121 gatgaattga aagccgctgt tgaagcaaac aacatcgaag aaatgaaaac taaacgcgat
    12181 gcattgaacg aaatcgttca aaacttgact gttaaactat acgaacaagc ggcacaacaa
    12241 caagctcaag aaaacccaga agcagctcaa ggcggagcag atgatgtcgt tgatgctgat
    12301 tttgaagaag tcgatggcga cgataaataa tcagttcact gaatttcgag agaaggccaa
    12361 agttgctact ttggcttttt cttttgaaaa atagtttgaa gtatcggttt ttgtttcaaa
    12421 caaaatatga tatgataaca agcgacgtga aaatgatgag aaccaatagg aggaaagcct
    12481 atggcaacaa aacgtgatta ttatgaagtc ttagggctct caaaaggagc atcagaagat
    12541 gaaataaaga aagcttatcg taagctttcg aaaaaatacc atcctgacat caataaagaa
    12601 cctgatgcgg aagaaaaatt caaagaagta tcagaagcat atgagatttt aagtgacccg
    12661 caaaaacgcg ctgcatatga ccaatacggg catgcaggca cagatccaaa ttacggtgct
    12721 ggtggagctg gtggcggatt tggtggattc ggcggcttct ctggtggcgg cttcggtggt
    12781 tttgaagata tcttcgactc tttctttgga ggaggcggtc ggtcagtcga tccaaatgcg
    12841 cctcgtcaag gtgctgattt gcaatatacg gtcgacttgt cttttgaaga agcgattttt
    12901 ggtgtagaaa aagaaatcaa gtataatcgg gaagaaatct gtcatacttg tggtgggaac
    12961 ggtgcaaaac caggcacaca accaactact tgtcataaat gtcatggtgc aggtacgatt
    13021 aatgtagaac gccaaacacc acttggacga gtgatgagcc gtcaaacttg tgatgtttgt
    13081 catgggacag ggaaagaaat caaagaacct tgtccaacct gtcatggctc aggacatgag
    13141 aagaaagcac attcagttaa agtgaatgta ccagctggtg tagaagaagg acaacaaatg
    13201 cgtttagctg gtcaaggaga agctggagaa aacggcggac catttggtga tttgtatgtg
    13261 gtcttccgtg tagaagaaag tgatatcttt gatcgtgaag gttctgaaat ttactatgaa
    13321 ttgcctttaa gttttgtaca agcagctttg ggggatgaag tccaagtacc aactgtccat
    13381 ggagatgtga aattgaagat ccctgctgga acacagacag gtacgaactt ccgtttacgt
    13441 ggaaaaggcg ctccgaagtt acgtggtggt gcaacaggcg accaacatgt caaagtaaaa
    13501 ctgatcacac cgaaaaatct aaatgaacaa caaagagaag cattacgtgc attcgctgaa
    13561 gctggcggca tcaaagttga agaacagcaa gaagacggtt tcttcgataa gtttaaagat
    13621 gcttttggcg ggaaaaagcg gaagtagttt ttctagtaaa aacgctaagt cattaataat
    13681 taaaaaccag acgaacaatc atatgttcgc ctggtttttt tgtagttgtc atgtttgtac
    13741 caacaaaaaa gccagcttta gaatatgata atcgtcatag tttctagggg tataaccagt
    13801 gagagaatgg aacttgttca atcgatattg caaggtgttt ttatgaataa ataattcttc
    13861 tgagcaattt ttgatgcttc cgttattttt actgaagcat gccaaagtct tcttgaacaa
    13921 atgtacttct tcttctgtca ggcttttcaa aatacgtttt acatataatg ttcgttgatc
    13981 tgacgggatt gctggtaaaa cgatcccgag gtctaaatct tcaaaaaagc ggatctcgtt
    14041 cacagattga tggaaagcta tccattcgca aacttgtttg gcatgaagga aagattgcgg
    14101 tgtctcttgc tctgtataac aaagcgaccc aattccaaag tttagctgta actgatacat
    14161 tttattaaac tttgtctgga ttttatcaag caatgatagc aatgaggctc tggaaatccg
    14221 attgactagg ataatgattt gttgtctatg tacagtgagc aaagcctgtc cgtaataaga
    14281 gaagaccctt tcgagcaact ccaataaagg attgatccgt tcttgacaca attgctgatt
    14341 gtcagaaagt tgtcccacaa ctacataata tggagaagaa aaatcaagct caattaagtg
    14401 agggtggtat cgactatgta cttgatatca tcaatgaacc gatggtactt tcttatgcga
    14461 tggaacaggt agagcgattg cttgtttgtg caggaaaaca agcgatgcgg ctgatacgat
    14521 taggtgagaa aataagaaga taaaaataca cggtcccttc atggcgaccg tgcatttttg
    14581 gtttttatta aatatttaat actttattca agaaatcttt tgtccgttca ttttgcggat
    14641 tttcaaagat ttgttgaggc gttccatctt ctaaaaagtt tccgtcgtca atgaacatga
    14701 cgcgattggc cacttcttta gcgaacccca tttcgtgcgt aacgataacc atcgtcatac
    14761 cttgttctgc taactttttc atgacgttca agacatcacc gaccatttca ggatcgagtg
    14821 cagaagtagg ctcatcaaaa agcatgatgt cagggttcat cgctagagca cgagcaatag
    14881 ccacacgctg tttttgtcca ccagaaagca tttcaggata catatctttt ttatcttcta
    14941 gaccaactgt ctgcagtaat ttgattgctt ttttctctgc ttcttctcta gattctttct
    15001 tcaggtccat cggtgctaaa atgatgtttt ccaaaataga aagatgaggg aataggttaa
    15061 agtgttggaa taccattccg atatgttgtc tcaccaagtt gatatctgtg cttttatcca
    15121 tcaagtgggc gccgtcaata ataatttcac cgtcagaagg ttcttctaaa cgattcaagc
    15181 aacgcaagaa tgtacttttt cctgaacctg aagggccaat gatacagacg acatctcctt
    15241 cgttgatgga tacattgatg tcgtttaata ctgtgttatc gccgtatttt ttgactaaat
    15301 gctcaactaa gattttttct gccatcttac ttcaccttct tttctaaaac ttttgctaat
    15361 tttgtcaatg ctgtgatcaa aatcaagtac atgatcgcaa tgatcaagta aacatacgta
    15421 ctttgtgttg tacgtgcaac aatgatctta ccagtttgca gcaattcgac tacaccgatt
    15481 gcggaaatga tcgttgtgtc ttttaatgag ataacgaact gattgacgaa tgatgggatc
    15541 atgatgcgta tcgcttgcgg caaaatgatt ttttgcattg tccgattata agaaagacct
    15601 aaacttctgg aagcttccat ttgtccgaca ggaacggcgt tgatcccgcc tcgaacaatc
    15661 tctgcgatat aagcacttgc gttaagagtc aaggtgataa caccagcaag gaagtcagga
    15721 atcgtaaatc caacgatacc aggcaaccca aagaaaataa agaacgcaag aaccatcatt
    15781 ggaattcctc ggatgatatc gacataaata ctagcaattg tgcgaagagc gcgtattggt
    15841 gctacactga ataatccaaa aatcacaccg attactatag ccaaagcaaa ggagatcaaa
    15901 gttaggagaa ttgttctcca aagtcctttc aacaatgctt gatagttgtt ttggataagt
    15961 ccaagaaatg ttgattcatc agcagtggaa gactcactgg aagtttctga accatccgct
    16021 acatattctt taataatctt atcgtattcg cctgttcttt ttaattcttt caatccttca
    16081 ttgaacatat taagaagctc aggattttgt ccttttttaa cggcaaatgc atattttccg
    16141 ccagattctt ttttgatcgg tgtttccaat tcttgtcctt gtttgatcgc ataaccgatt
    16201 accggataat catccatcat tgcatcgatt gcgccaactt ttaacccgtc gtacaattga
    16261 tcagcagcat caaaatattt gatagaatag ccatattcag attcatggtc gcttaaaaat
    16321 tcagcacttt cggtaccaac ttttgctccg actgtttttc ctttcaaatc ttcataagac
    16381 ttgatcgaag tatctccttt tttgacagct aattggatcc cgctatcaaa gtaagaatca
    16441 gaaaaatcat agcttttctt tcgttcatct gttacagtca ttccggcaat cattccgtct
    16501 gcttgtcctg attccaaagc ttgcaaagca gcagagaaac cacggtagtc cattcggatg
    16561 ttaaatcctt gcagtttggc gatgttttcc atcaatttta catcgatgcc ttcgtactcg
    16621 ccctttgcgt tttgaaattc aaatggagca aaagcagagt cactggcaat tacgtatacg
    16681 tcttttttgg gctctatttt ttgcattgtc tcgcttgtgc ttgactctgc cgttgtagtg
    16741 ttgtcagctt gtgcaacatg accaaacgca agtgtcacaa gtcctgtcat taagaaaaca
    16801 aatgagtgaa ataaatgctt tcttttcata atcaacctcc taaatttcca atattttatt
    16861 attataggcg aaaagtgctg aatcagacaa gactgtaagc tcgaaaaaat gaaaaaaatg
    16921 cgagtttcta acattttatg ttataaaaca taacatgtat aataagataa aaaagtggcg
    16981 aaagtatgtt cggtgtggta taatttttta gtaaggaatg cctttgcttt ccttttcttt
    17041 ttattggtcg taaaatgaaa aggaatgaat gaaaaggagg acgaagaatg attgagagag
    17101 atacctcccg acactttgag cttgtctcga aataccaacc ggctggtgat cagcctgaag
    17161 cgattaacca attagtggat ggcgtagtcg gcgggaaaaa agcacaaatc ttattaggtg
    17221 cgactggaac agggaaaaca tatacgattt ccaatcttat tgagaaagtg aataaaccaa
    17281 cactgattat tgcccataac aaaacattgg ctggtcagct gtacggggaa ttcaaggaat
    17341 ttttccctaa caatgctgtt gaatattttg tttcttatta tgattattat cagcctgaag
    17401 cttatgtgcc ttcaagcgat acgtatatcg aaaaagattc aagtgtaaat gatgagatcg
    17461 acaaattacg acattcggct actagttcat tattagaaag aaatgatgtg atcgtcattg
    17521 catccgtttc atgtattttt ggattaggct cgccttttga gtaccaaaaa caagtagttt
    17581 cgattcgaca gggagcagaa ctagatcgca atcagttgat tcgtgactta gtcagtatcc
    17641 aattcgaaag aaatgatata gattttcagc gcggtcgttt tcgagtgcgc ggtgatgtcg
    17701 tggagatttt tccggcttca agagatgaac gtgccttacg tgtggagttt tttggagatg
    17761 aggtcgaacg tatccgagaa gtcaatgcac tgacaggtga agttttagga gaaacggaac
    17821 acgtagcaat cttcccggcg actcactttg tgacgaatga tgaacatatg gagcatgccg
    17881 tagcgaatat caaagcggaa ttagaacagc gtttaactgt cttgcgaaat gaaaacaaac
    17941 ttctggaagc tcaacgttta gaacagcgaa ccaattacga tattgaaatg atgttagaaa
    18001 tgggctacac gtcgggaatt gaaaattatt ctcggcatat ggacggacgt aaagaaggtg
    18061 aaccgcctta tacgctattg gatttctttc ctgaagattt tttgatcgtg gcggacgaat
    18121 cgcacgtaac catgcctcaa atcagaggaa tgtataatgg tgaccgagcg agaaaacaga
    18181 tgctagtaga ttacggtttc cgtttgccat ctgctttaga caatcggcca cttcggttag
    18241 aagagtttga aaaacatgtc aatcagatca tttatgtctc tgcgacgcca ggaccatatg
    18301 aacatgaaca aacagatacc gttatccaac aaatcatccg tccgacaggg ctgcttgacc
    18361 cagtgatcga agttcgtcca atcatgggac aaatcgatga tctagtaggc gaaataaatg
    18421 aacgagtcga aaaagaccag cgggtgtttg taacgacatt gacgaaaaaa atggcagaag
    18481 acctcacaga ttatttcaaa gaattaggga tcaaagtcaa atatttgcat agtgatatca
    18541 aaacgctgga acgaacagaa atcattcgag atcttcgatt aggtgaattt gatgtgttag
    18601 ttggaatcaa tttgcttcgt gaaggattag acgtacctga agtttccctt gttgctatct
    18661 tggatgcgga taaagaaggc tttttacgta gtgaacgttc cttagtccaa acgattggac
    18721 gagctgctcg gaatgaagaa ggtaaagtaa tcatgtacgc agacaaagta acagattcta
    18781 tgcgtctagc gatggatgaa acgtcaagac gtcgtacgat tcagcagaag tacaatgaag
    18841 aacatgggat cgttccaaaa acgatcatta aagaaatccg agatttgatt tctattacga
    18901 aagaatcgga agatgataca aaagaagcgg tacaagtctc gtacgaagaa atgacgaagg
    18961 aagaaaaaga cacgttattg atgaaattag aaaaagaaat gaaagatgcg gcaaaagctt
    19021 tggattttga aacagccgcg aatgtgcgag atatgatttt agaattaaaa gcttcaaagt
    19081 aggaggacaa catggctaac gataaaatta cgattcatgg tgcacgtgcc cataacttga
    19141 aaaatataga tgtaacgatt ccacgagata aattcgtggt cgttacaggt ttgtccggtt
    19201 ctggaaaaag ttccctagca tttgatacgc tgtacgctga agggcagcgg agatacgtag
    19261 agagcctttc tgcttatgct cgtcagtttt taggacaaat ggataagccg gacgtagata
    19321 gcatcgacgg attgagtccg gcgatttcta ttgatcagaa aacaacaagt aaaaatcctc
    19381 ggtcgacagt aggtactgtg acggaaatca atgactattt acgcttgttg tttgcacgtg
    19441 taggacatcc gatttgtccc aacgatcata tcgagattac cagtcaatca gtagatcaaa
    19501 tggcagatca agtaatggct ttgcctgagc gcacaaagat ccaagtgttg gcaccgatcg
    19561 tggtaaagaa aaaaggacaa cataaaaaaa tattcgaacg aatccaaaaa gagggatacg
    19621 tccgcgttcg agtcgatggt gagatctatg atttaagtga agtgcctgag ctggagaaaa
    19681 acaaaaaaca tgatatcgcg atcgtcatcg accggatcat tgtgaaagaa gggattcgtt
    19741 ctcgcctatt tgattcttta gaggcggccc tgcgattagc agacggctat gctctggttg
    19801 acgtgatcgg tgaagaagaa atgttgttta gcgaacacta tgcctgtcct tattgcggat
    19861 ttactgttgg cgaactagaa ccaagactgt tctcattcaa tgcgcctttt ggagcttgtc
    19921 cagactgtga tggtttaggt gtcaaattag aagtcgatca agatttagtg attccagata
    19981 gaacgaagac acttagtcaa ggtgctatcg ttccatggaa tccaatcagc tctcagtatt
    20041 atcctcaaat gttagcgcag gcttgtgaaa gtttcggtat cgatttagat acaccattcg
    20101 aagaattacc ggaagagcat caggagatca tcttgaatgg gacagatgat cttttccatt
    20161 tccattatga aaatgatttt ggtggcgttc gcgatgtaga aacgactttc gaaggggtat
    20221 tgaagaatat cgagcgtcgt tatcatgata cgaacagtga tttcacgcgt gagcaaatgc
    20281 gtctttatat gacagaatta acttgtcaga catgtaaagg ttatcgattg aatccccaag
    20341 ctctatctgt tcaaatcaat ggaacgaata tcgggcaaac aaatgagttg tctattcaaa
    20401 aagcagtaga tttcttctca aacttaacgt tatctgaaca agagaaagta attgccaaac
    20461 cgattttaaa agaagtaaat gacagacttt cattcttgga aaatgtggga ctagactatc
    20521 taactttgag tcgcgcttcc ggaacattat ctgggggaga agcgcagcga atcagattgg
    20581 ctacacaaat tggatcgaat ctttctggag ttttgtatat tttagatgaa ccatcgatcg
    20641 gattgcatca acgcgacaac gatcgactga ttggctcatt aaagaaaatg cgtgatcttg
    20701 gcaacaccct cgttgtagtc gaacatgatg aagatacgat gtgggcagca gattatttga
    20761 ttgacgtcgg acctggggcc ggagaacaag gcggacaaat cgtggctgca ggcactcctg
    20821 aagaagtagc taatgatgaa aattctttga cagggaaata tctttcaggg aaaaaatcaa
    20881 tccctgtacc aaaagaacgc cgtaaaggta atgggaaagc aatcaagatc actggtgctt
    20941 cagaaaacaa tctgaaaaat attagtgttg aatttccatt aggagaattt gtagcagtca
    21001 caggtgtgtc tggttctgga aagagtacat tggtcaatag tatcttaaag aaatcgttag
    21061 cgcaaaaatt aaataagaat tctgctaagc caggtaaatt caagacaatt tccggctacg
    21121 aaagtatcga aaagatcatc gatatcgatc aaagcccaat cggccggacg ccgagaagta
    21181 atccagcgac ttatacaagt gtatttgatg atatccgtgg gttatttgct caaacgaacg
    21241 aggcaaaaat gcggggttat aagaaagggc gttttagttt caacgtaaaa ggcggtcgtt
    21301 gtgaagcttg tcgcggggat ggaattatta agatcgaaat gcactttttg cctgatgtct
    21361 atgttccttg tgaagtatgt catggtaaac gatataattc tgaaacatta gaagtgcatt
    21421 acaaaggaaa aagcattgct gatattttgg aaatgacagt agaggatgct gtagaattct
    21481 tcaagcacat tccaaagatt catcgcaaac tgcaaacgat tgttgatgtt ggcttaggtt
    21541 atgtgacgat ggggcaacca gcaacgacat tgtccggtgg tgaggcacaa cggatgaaac
    21601 ttgccagtga attgcacaaa atctctaatg gaaagaattt ctatatacta gatgaaccaa
    21661 cgacaggact tcatagcgat gacatcgccc gcttgttgca tgtattacaa agattagtag
    21721 atgctggtaa cacagtttta gtgattgaac acaatctaga tgtaatcaaa acagcagatt
    21781 atatcattga tttaggacca gaaggtggag aaggtggagg aacgatcctt gcgactggaa
    21841 caccagaaga aatcattaac gtaaaagaaa gttatacagg tcactatttg aaaaaaataa
    21901 tggtataaag aggatagata gctgagatag ttaaaaaaac ataaaaaaat gaatttttgc
    21961 ttgttttatt aatatcagtg ttctagaatc attacatggt agttctttct acactgattg
    22021 gtatagataa aaacttccta taagttcaat ccgttcaaaa aagaattgag caatcggagg
    22081 actttccttt gattgctttt ttctttatgg tatgatattc cttgtgtagt tggaaaagaa
    22141 aaacaagaga ttatgagtaa gtgagagata atgatgaaca gtattgaaaa tgaagaattt
    22201 atccctatat tattaggaag cgacatgaac gtttatggta tggcacgttc ttttaatgaa
    22261 gcatacggca aaatctgcca agcatatgct tcggatcagt tagcccctac ccgctacagt
    22321 aaaattgtca atgtagaagt tattccagga tttgataaag accctgtatt tatcgagacg
    22381 atgcttcgtt tggcaaaaga aagatattct gataagagta aaaaatatct tttgattgct
    22441 tgtggtgatg gatatgcaga attgatctcc caacataaac aagagctgtc agaatatttt
    22501 atctgtccat atattgatta ttctttattt gaaaggctga tcaataaagt aagcttttat
    22561 gaagtttgtg aagaatatga tcttccgtat cctaaaacat tgatcgtcag agaagaaatg
    22621 ttagtaaatg gacatttgga acaggaattg ccttttgaat tcccagttgc tttgaaacct
    22681 gcaaacagtg tggagtatct ttctgtccaa tttgaaggaa gaaaaaaagc ttttatttta
    22741 gagactagag aagagttcga tttaatttta ggccggatat acgaagccgg ttataaaagt
    22801 gaaatgattg tacaagattt tatcccgggt gatgatagta atatgcgtgt gctaaatgcg
    22861 tatgtagatg aagatcatca agttcggatg atgtgccttg gtcatccact attagaagat
    22921 cctacaccag catcgattgg taattacgtt gtgatcatgc cagattataa cgaaaaaatc
    22981 tatcaaacca ttaaggcatt tttggaaaaa atcgaatata caggctttgc caatttcgat
    23041 atgaagtatg atcctagaga tggagaatat aaattatttg aaatcaattt gagacaaggt
    23101 cgtagcagtt tctttgtgac attgaacggc ttgaatctgg ctcgttttgt cacagaagat
    23161 cgagtgttca acaaaccctt tgttgaaacg acctatggaa cgaatcaatc agataaagca
    23221 agattatgga tgggtgtgcc caaaaaaatc tttttagaat atgctcggga aaatgaagac
    23281 aaaaaactag ctgagcaaat gatcaaagaa aatcgttacg gtactactgt attttatgaa
    23341 aaagaccgat caatcaagcg ctggttgttg atgaaatata tgttccataa ctatattcca
    23401 agattcaaaa agtattttca tgtgaaagaa ggctagttca tggagaattt tttcagtatt
    23461 ttaggcggaa tgggcacgat ggcgacagaa agttttgttc gcttgatcaa ccaccgaacg
    23521 aaagctacaa aagatcaaga atatttgaat tatgtcttat tcaatcatgc aacagttcct
    23581 gatcgtacag cttatatttt agatcgatca gaagaaaatc caatgccgtt tctgctggat
    23641 gatattgaga aacaaaattt attgcgacca aacttcattg tgttgacttg taacaccgca
    23701 cactactttt ttgaagagtt acaagcggca acggatattc ctatcttgca tatgccaaga
    23761 gaagcagcaa atgaacttgt ccgtcagcat acgacaggga gagtagcgat tctaggaaca
    23821 gaaggaagta tgaaagctgg aatctatgaa cgcgaagtca aaaatcttgg tttcgaaaca
    23881 gtgattcctg atactgcgct tcaagaaaaa atcaattacc tgatctatca tgagatcaag
    23941 gaatcggatt atttgaatca agagctctat tacgaaatct tagaggaagc ggtagagcgc
    24001 ctaaactgtg aaaaagtcat cctaggttgc acagaattat ctttgatgca tgaatttgct
    24061 gaagataatc attacccagt gatcgatgct caatctattt tagcggatcg aacgatcgaa
    24121 cgtgcattag ctgaaagaag cgaggcattg gatacagcat cggaaaagta aaaaaattgg
    24181 atttggggca aatatcctaa aagatatttg gtccaaatct ttttgtaaaa gaaaaagaaa
    24241 actggtaatg atacgcccgg gatgatataa ttttattatg cacaatgtga gaggggttcg
    24301 taaatatgac agataatttg caattagtca tcataacagg tatgagtggt gcagggaaaa
    24361 cagtagctat ccaaagtttt gaagatatgg gctatttttg tatcgataat atgccgcctc
    24421 gattgattcc aaaattctgg gaattgatca aagaatcagg taaagtcaca aaaatggctt
    24481 taggagtgga tttgcgttct cgttccttct ttgaagaaat ccaaaacatg ctgatcgaaa
    24541 tcgaaaatac gaacttcatc gacacttcta tcttattttt agatgcttca gacgaagaac
    24601 tagtggctcg ctacaaagaa acaagacgag cacatccaat ggcgatggac ggtttgatca
    24661 ctgaagggat acgtaaagaa cgggcgatac tagacgactt gaaagcccaa gcttccgtga
    24721 tcattgatac gacaacattg acaccacgac aattacgtga aaaaatcaat gagtcattca
    24781 aatcaaaggg agaccaagga tttcggatcg aatttgtatc atttggtttt aaatacgggt
    24841 tgccgatcga ttcggatatt gtgatggatg tgcgtttttt gcccaaccct cattatatcc
    24901 cggaactaag accattgacc ggaaaagatc agccagtcta tgattatgtg atgtcctttg
    24961 atgaaacgca atcattctac cataaatttt tggatcttct ggaaactgta ttgcctggat
    25021 acattaaaga aggaaaaagc agcttgacag ttgcaatcgg ctgtacaggc ggtcaacacc
    25081 gttctgtggc attgactgaa cgtttggcaa atgctttagg gaaaaactat aaagtcaatg
    25141 tgacacatcg ggacaaggat aaaaggaaag agacggtgaa ccggtcgtga aaatgaaaac
    25201 ctaccggata agaaaaccaa agatcgtagt ggtcggcggt ggcactggtc tgccagttat
    25261 cctgaaaagt ttacgaaatc aaggagcaga tatcacagca gtagtgactg ttgcagatga
    25321 tggtggaagc agcggtgcaa tcagagaatc gatcgctatg gctcctcctg gtgacttgag
    25381 aaatgtctta gtggcgttat cggatatgcc acaattctat gaagatattt tccaataccg
    25441 tttcaaaaag gaagatcagt ttttagccaa tcatacgatc ggtaatctga tcattgccgc
    25501 agtttccgag atgcggggaa gcacttatga ggcgattcaa ttattagcaa aaatgatgca
    25561 tgtagatgga catgtttatc cttcttcaga aactcctttg accttgcatg ctgtttttaa
    25621 agacggaacg actgcagtag gagaatctaa aatcgcgatc aaccgtaaaa ccattgaccg
    25681 tgtgtatgtc tgcaacacaa atgataaaag tgaagccaaa gcggctcgaa aagtcgtttc
    25741 agcaattatg gacgccgaca tggtcgtttt gggacccgga agtttattca ccagtatcct
    25801 tccaaattta gtgataccag aaatcggcga agcaatgctc aagacaactg ctgaaaccgt
    25861 ctatatctgt aatatcatga cccaaaaagg ggaaacggaa catttcacag atgccgatca
    25921 tatacgtgtt ttacacaagc atttgaatca gccttttatc gatacagtat tggtcaatac
    25981 ggaaaaagtg cctgaagatt atatggaccc agagatctat gatgagtatc tagtacaagt
    26041 cgcacacgac ttcaacggat tgagaaatga aggatgtcga gtgatttcca ccgacttttt
    26101 agaattgaaa gatggtggtg tcttccacga tggagacaaa gtagtagaag aattgttccg
    26161 tttagttttc ggttcaaaat attaagcaat ttattgttgg agaaaggagg agaataggat
    26221 gtcatttgct gcagatgtga agaaagaact aactggtttg gaagttcatc gagaacatgc
    26281 gaaagcggaa ttagctgctt tgctgcgaat gaacggctct ttaggtattg tgaaccagca
    26341 gtttattttg aacgtccaaa cagaaaatgc tgcaattgcc agacgtatct attctctttt
    26401 gaaagatcat tatcaggtac gaagtgagct actcgttcgt aaaaaaatga aattaaaaaa
    26461 gaacaatgtc tatatcgtcc ggttgaagca aggcacaaaa gatgtgttga tggatcttga
    26521 tatcatggat ggtatgatgt tcaatggaca cgtttcggac gagattatgg gaaattcgca
    26581 aaaaatgcgt tcttacttac gtggtgcttt tatggcctca ggttcagtca ataatccgga
    26641 aacgagtcgt tatcatttgg aaatattttc tatttatgaa cagcataacc aagacatttg
    26701 cgatatgtta aattattacg gtctacatgc tcgaacgctt gaacggcgca atggctatat
    26761 ttcgtattta aaaggagcag aaaaaattgc tgatttctta acattgattg gtgcaaccaa
    26821 ctcaatgctc cgatttgaag atgttcgaat cgtacgggat atgagaaatt cggtcaatcg
    26881 tctagtcaat tgtgagacag caaatctgaa caagacgatt gatgcggctt ctaaacaaat
    26941 cgaaaacatc gaattgatcg aagcaaaggt cggtctacat gcccttcctg aaaaacttca
    27001 ggaaatcgca gaacttcggc ttcaacatcc agaggtcagc ttgaaagaat taggggaaat
    27061 gattccctca ggtgcaatat caaaatcggg aattaatcac cgaattcgca aaataaatga
    27121 atttgcggaa aaactgcgtg aagaagcagt ttaatccaaa gaaaaaactg atacatgaag
    27181 caggagatgc gaatgtatca gtttttttta aaagctatac ttgcttttct tccgtcaaac
    27241 tataattcca actttactgg agtaacataa tgttgaaagg cttctattgt tttttaatac
    27301 aatcgtataa aaagcgggga gacaactgaa tgaagatttt agttttatac ataaactttt
    27361 gcttaatata gaagtcctga tatagatagg atcaagtatt tatctataac tggagattaa
    27421 ttaaaaactg gaaggaactg ctcgatcaac cttgagaact gtttttctta cttctggtca
    27481 agattctcgt ggttggtgga taaggatggt tgataagttc attggattag ctagtcgaaa
    27541 acaaccaggt gtaattaatg tagaagtttt aacaagggga tggagtgatt tattcagtga
    27601 taatcataag ttcataaata gtaattggga tgtatggttg gtatacgtta tacctgaact
    27661 tgaactatca agatttggtt tagaatttga gaatcgatgt ttataaaggg agggcaaaaa
    27721 atgtgctttt ttacaacttt tactatcatt atgattgcag tactttttat ttttctagat
    27781 atagcaaagc gaaatacagc atttttatta tatcgggttt tacttcgagc aggtctcata
    27841 acatttatat cgatcgttgg attttttcta ttcacagtta ttgtatttat ttggagaact
    27901 acagcaccac cattgccaga gattacatat ggagaatttc cgtttagtct tgaatatgaa
    27961 atagatgagg aaagaatttt gattgaagat acggtgattg cagaatttat aggaagtttt
    28021 agagggaatg tgacaactca acctagacga agttggagta taaaactttt aaataatagt
    28081 ggagagttga aagcatttca ttatgaattc ttaataatag aaagtgaaga tgtaacaatc
    28141 actttaagta gtggctctgc tggatatttt atgaatgatg gtggaagcaa gccacagctc
    28201 acagtccgtg atcgtattgc aggttttatg catttttctc actatgactt agaagaagct
    28261 cgtaaaatac ttgcagaata tgggatcaca ttaatcaatt gggaatatgc gccaccgata
    28321 agaaattttt tcaatgatta ttattagtct tataaaaaac agaatgtaaa atttatgtgc
    28381 gcgagtattt agttttccat tttttgtttg atagatatat agcattgttg aagcatggga
    28441 tagtggtgcc aattttatca atgaggttgg tcaggcagtt aacataattg gtgatgctat
    28501 aaatccattt aattggaatt ggaggaatta ggtgtggaaa cagtgagatt atggatattc
    28561 atgaaaaatt ttcgttcggc tttgagatat gttattctgg aatcaaaatc aggttattat
    28621 ctgattgaca cagatcacta tactttttgg ggaggctacc tttttccgat ttttaattgg
    28681 ataatccccc ataaggttat gaaaattaac ttgcctgcag aagaaataaa tagattaata
    28741 ctcaacaaag atttacaaag gaaaagagag aaagaacatt ctaatgttgc ttggataggt
    28801 ccagcatttg cagtagtgac ttcttcaatt gtcggtccta tgctagagag tatttatagt
    28861 gatttttcta ttggaatacg ttatttattt gttactttaa gtatagctgt tcttttgtgc
    28921 ataaagggtt atgttagcaa gaagaataaa gatattttaa cactaatcgg tgtaaacaat
    28981 ttatctagta ctaaaattat actgtttcct gaatctataa aagaaggatc aaggttgttg
    29041 ataattttta taatgcttat actgttttct gttttatttg gggtgatgtt tataga
//
LOCUS       DACCYH010000030        27669 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.030, whole genome shotgun sequence.
ACCESSION   DACCYH010000030 DACCYH010000000
VERSION     DACCYH010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.030"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            238..2004
                     /locus_tag="IVY85_001255"
     CDS             238..2004
                     /locus_tag="IVY85_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP8044282.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2106..3365
                     /locus_tag="IVY85_001256"
     CDS             2106..3365
                     /locus_tag="IVY85_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8044283.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3512..4813
                     /locus_tag="IVY85_001257"
     CDS             3512..4813
                     /locus_tag="IVY85_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8044284.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4816..5667
                     /locus_tag="IVY85_001258"
     CDS             4816..5667
                     /locus_tag="IVY85_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8044285.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5680..6495
                     /locus_tag="IVY85_001259"
     CDS             5680..6495
                     /locus_tag="IVY85_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP8044286.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6646..7812
                     /locus_tag="IVY85_001260"
     CDS             6646..7812
                     /locus_tag="IVY85_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8044287.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8042..8689
                     /locus_tag="IVY85_001261"
     CDS             8042..8689
                     /locus_tag="IVY85_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP8044288.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8773..9717
                     /locus_tag="IVY85_001262"
     CDS             8773..9717
                     /locus_tag="IVY85_001262"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP8044289.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9852..11303
                     /locus_tag="IVY85_001263"
     CDS             9852..11303
                     /locus_tag="IVY85_001263"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP8044290.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11324..12160
                     /locus_tag="IVY85_001264"
     CDS             11324..12160
                     /locus_tag="IVY85_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8044291.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12160..12819
                     /locus_tag="IVY85_001265"
     CDS             12160..12819
                     /locus_tag="IVY85_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8044292.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            12945..13634
                     /locus_tag="IVY85_001266"
     CDS             12945..13634
                     /locus_tag="IVY85_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044293.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13640..14596
                     /locus_tag="IVY85_001267"
     CDS             13640..14596
                     /locus_tag="IVY85_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP8044294.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14640..15152
                     /locus_tag="IVY85_001268"
     CDS             14640..15152
                     /locus_tag="IVY85_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8044295.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15408..15956)
                     /locus_tag="IVY85_001269"
     CDS             complement(15408..15956)
                     /locus_tag="IVY85_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8044296.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16045..17226)
                     /locus_tag="IVY85_001270"
     CDS             complement(16045..17226)
                     /locus_tag="IVY85_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP8044297.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17462..18970)
                     /locus_tag="IVY85_001271"
     CDS             complement(17462..18970)
                     /locus_tag="IVY85_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP8044298.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19129..19320
                     /locus_tag="IVY85_001272"
     CDS             19129..19320
                     /locus_tag="IVY85_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044299.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19523..20440
                     /locus_tag="IVY85_001273"
     CDS             19523..20440
                     /locus_tag="IVY85_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP8044300.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20433..21506
                     /locus_tag="IVY85_001274"
     CDS             20433..21506
                     /locus_tag="IVY85_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044301.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21654..22172
                     /locus_tag="IVY85_001275"
     CDS             21654..22172
                     /locus_tag="IVY85_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044302.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22153..22545
                     /locus_tag="IVY85_001276"
     CDS             22153..22545
                     /locus_tag="IVY85_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP8044303.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22621..23556
                     /locus_tag="IVY85_001277"
     CDS             22621..23556
                     /locus_tag="IVY85_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP8044304.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23689..24468
                     /locus_tag="IVY85_001278"
     CDS             23689..24468
                     /locus_tag="IVY85_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP8044305.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24461..24778
                     /locus_tag="IVY85_001279"
     CDS             24461..24778
                     /locus_tag="IVY85_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8044306.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            25022..26632
                     /locus_tag="IVY85_001280"
     CDS             25022..26632
                     /locus_tag="IVY85_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8044307.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26789..27637
                     /locus_tag="IVY85_001281"
     CDS             26789..27637
                     /locus_tag="IVY85_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8044308.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         8960 a         4890 c         5553 g         8266 t
ORIGIN      
        1 aaaactttta ctttagaaaa ttggagaaat tgatcgctta agataaattc aggacactaa
       61 atagtacagg aagtagatta aaacttttaa aagttttaag tctatctatt tggcaagttt
      121 aaaaaataaa aattcggtat caaaaaactg ttaccgataa catttttgaa agcgttgtct
      181 tttgtttctc tatcacttat agtgatgacg tcatattaaa acacaaggag gaaatcaatg
      241 gatacagctg caatttatca tcgaccggaa agtgaatatg catttctata tacgaataca
      301 agatttcgta ttcggcttcg tactaggaaa aatgatatca aaaaagtata tgtgctttgt
      361 ggagatccat ataccataac aactgaaaaa tggtatcaaa aacaacgacc aatgaaaaaa
      421 tgtctaagta ccaatgttca tgattattgg gaaattgaag tgacgagcga aacaaggcgc
      481 ttacagtatg cgttccatgt tgttggggaa gataacacgg actgttttta cggtgatcaa
      541 ggaatttttc cttatcgaga aaatgtgatt actgaaccaa atttttattt tcgaatacct
      601 tattttcatc aaatcgatcg attcactatt ccagaatggg taaaagaaac agtatggtat
      661 caaatttttc ccgaaagatt tgcaaacggg gacaaagcaa atgaccccga acacacttta
      721 ccttggggaa gtaaagatcc agaccgagaa gacttcttcg ggggagattt gcaaggtgtg
      781 agggatcatt tggattactt gacggacctt ggcgtcaatg gcatctattt ttgtcccata
      841 tttaaagcca catccaatca taaatacgat acgattgatt attatgaggt ggatcccgct
      901 tttggcgata aaaaactttt aaaaaacttg attgatgaag cacataaaag agggatacgt
      961 atcatgttcg atgctgtatt caatcatatg ggtgtccatt caccacagtg gcaagacgtt
     1021 ctcaaaaacg gagaaaaatc gatctataaa gactggttcc acatccgttt tttcccagta
     1081 gattcttatc agatgactga cttaccagaa actgctgaaa atatcccata tgatactttt
     1141 gcttttactc cattcatgcc gaaactaaat acagcaaatc cagaagtgca aaaatatttg
     1201 ctggatatcg caacttattg gataaaagaa ttcgatattg atggatggcg tcttgatgtt
     1261 gctaatgaag tcgatcatca tttttggaaa aaattcagag aagcagttac agaaatcaaa
     1321 ccagacatct atatccttgg agagatttgg cattcctctc aagcttggct gcaaggggat
     1381 gaattccatg cagtgatgaa ttatgcattc acagattcga ttaaagatta ttttgcaaaa
     1441 aagaaaatca cagcttcaca aatggtcagt ggaatgaatc atcagcaaat gctttacaga
     1501 gatcaggtga acgaaggaac attcaactta ttagattctc atgatactgc caggatcttg
     1561 accctttgcc aaggtaacaa agaattgatg aagtctgtga tggcctttat gtttcttcaa
     1621 aaagggtctc cttgtatcta ttacgggaca gaaataggga tgactggtga agatgacccg
     1681 gattgccgca aatgtatgat atgggagaag gaggaacaag atctagagct attcggtttt
     1741 ataaaagaac ttgtttctct aaggaaacag ttgtctaaga ttatttctga aggttctact
     1801 cagtggctaa tagtatatga tcgtgaagat aagctctatt tcacacggga actagaggga
     1861 cagatcatct atgtttactt taatcagtca aaagaacctt gggtagtaga acaggaaaat
     1921 gaggtgattc ttagtcaaaa ttgtcagttg ttagaagacg ggaaagcaga gatacgacaa
     1981 tacggatttt tgatcatgtg ttgaaaactg ttaccggtaa caacaagaaa gcgtttgcaa
     2041 atgaagattt gtgcccatta taataagggt gtaacagcac gttgaataca caggaggaaa
     2101 tgaagatgaa aaaaagaagt gtgaaacagt tgggtttagg gttaatctct ttaggaatct
     2161 taggcggatt agcggcttgt ggcaatggta actcttcagc agaaggggaa gccgatgaag
     2221 gaaaaacgtt aacagtttcc gttgattctg gatataaaga ttatataaac gaaataaaaa
     2281 acactttcga aaaagaaaac gatgtgaaaa tcaagctagt agaaaaggac atgttcgatc
     2341 agttggaatc attagcgtta gatgggcctg ctggaaaggg acctgacgtt atgatggcag
     2401 catatgaccg gatcggtgcg ttaggacagc aaggacatct ggcggaagta aaactgggaa
     2461 atgaagcagc ttacgatgaa acagacaaag cccaagtgac ttatgatgga aaaatctacg
     2521 gagaacctgc agtaatcgag acccttgttt tgtactataa caaagattta gtcgatacag
     2581 caccatcaac gtttaaagat ttggagaatc tatccaaaga cagtcgtttt gcttttgagt
     2641 cagaagcagg aaaaaataca ggatttttag ctaaatggac tgacttttat tattcctatg
     2701 gtttaattgc aggttacggt ggctatgtat ttggtgatga tggcacaaat ccttcagata
     2761 tcggtttgaa taatgcaggt gctgtagaag gaatctctta tgcaacagat tggttcaaaa
     2821 atgtttggcc aaagggaatg caagacataa aaagtgcagg agacttcgcc agccagcaat
     2881 tcatgtccaa taaaactgca gcaatcattg acggtccatg gcaagcgcaa acatacaaag
     2941 aaaacaatat caattatggt gtagccaaaa ttcctactct aaataatgga cagccttatc
     3001 aaccgtttgg cggaggaaaa ggatgggtag tcagcaatta tgctaaaaac aaagacctct
     3061 ctcaaaaatg gcttgattat gtaacgaatc aggaaaatca agaaaaattc tttgaaatgg
     3121 taaatgaaat ccctgcaaac caacaagcta gggaaacagc aaaggctaaa aacgatgaat
     3181 taacaacagc tgtgattgaa caatatgaaa cagctcaagc gatgccaaac atcccagaaa
     3241 tgggcgaagt ttggacaggt gcagaaaact tgatgttcga tgcagcatca ggttcaaaaa
     3301 caccaaaaga atccgcagat gaagcagtaa aaacaatcag tgaagccata gaacaaaaat
     3361 actgattaaa atgataaccg tccattttta taaaaaatgg aacaggtgct tcagataaaa
     3421 gtggcaagag cgagatggaa aattatcttg ctcttgttgc cattatctta aaagcagttt
     3481 gcggtttagc aagtttagag ggggcattca gatgcagcaa gataaaacaa gcaatatcaa
     3541 cgaaaaaaaa atcacgcttt gttcacttat tccagggatg gggcagtttg taaatggcca
     3601 agcttttaaa ggtatcgttt ttttcgccat attcgtccta ttcattggtc agatgcttct
     3661 gggcggattt catgcattgg taggattggt tactttggga agtataccaa tggaagatca
     3721 ttcattattt attttgattg aaggcacttt gcaattcatc attacaatca ttttcttgat
     3781 tttttacggt ttgaatcttt tagacgctta tcgagtagcg aagatgcgtc aagagggcaa
     3841 aaaaatcaat cggacgatga aggaagtcat acataatata ggtgatgaag gttttcctta
     3901 tctattgaca cttcccgctt atcttctcat gatttttacg atcattttcc cagtcctagt
     3961 tacattgttc actgcattta ctaattatga cttcaatcat attccaccag ccagtttaat
     4021 tgattgggta ggggctaaaa acttttttag tatcttcttt ttaagctcat atcgtaatac
     4081 tttttttgca gtcttttcgt ggaccgtgat ttggacttta tgtgcaacaa ctttgcaaat
     4141 cacccttggt attctcacag cggttgttgc tcaccagaaa tttattaaat tcaaacgtat
     4201 tttcggtatc attttcttat taccatgggc ggtcccagct tttattacta tcatgagttt
     4261 ttcgaatata ttcaacgata gcgtaggtgc catcaatact caagtgattc ctttcataaa
     4321 ccagcttcct tttgtagata tcagtgcaat ctcttggaaa acggacccga actggacgaa
     4381 agtagcaatt atcttagtgc agggctggtt aggatttccc tatatctatg tgatggttac
     4441 aggaatctta caatcgattt cagaagactt atatgaagca gcaaaaatcg acggagcaaa
     4501 tgcatggcag cgcttttcaa acatcacgct tcctgctgtc ttcctagtgg cagcgccaac
     4561 atttattaca cagtatacgg ggaatttcaa taatttctcc atgatctatc tgtttaacaa
     4621 tggtggtccc ggaagcgtag gcggaggagc tggttctact gatattctta tttcctggat
     4681 ctataaactt acgactggaa cttctccgca gtattctatg gcttcagcaa tcacgttgat
     4741 tatctccgtc atcgttatct gtgtgtcatt gctggtattt aaaaagacaa atgcgttcaa
     4801 tatgggggat taacaatgaa aaaatttcat tcgagtgctc gattccaaaa acgactttct
     4861 cactttttta cttacttttt tcttttaata ctgtcagtca ttatcatcta tccgcttttg
     4921 atcacagcaa gtactgcctt caagtcagga aatattgcag cttttagttt agatctttca
     4981 agtgactgga ctttaggaaa ctttactcgg ctattcagtg aaacacttta cgggacttgg
     5041 tataaaaata cattgatcat ttctgtccta acaatgatcg tacaagtgac tgtagtaact
     5101 ttggcgggct atacttatag tcgctatcgt tttatcggcc gcaaatcaag tttgatgttc
     5161 tttttagtga ttcagatggt accgactatg gcagcattga ctgcctttta tgtcatggct
     5221 ttcttattag aagcgctcga taagtactgg ttcttgacaa tgatctatat aggcggtgga
     5281 attccaatga atacttggct gatgaaagga tactttgaca ctgtaccaat agatttagat
     5341 gaatctgcta aacttgatgg ggcagggcat ttcaggattt ttttccagat tgtattaccg
     5401 cttgtcaaac cgatgctcgc cgttcaagca ttatgggctt ttatgggacc ttttggggat
     5461 ttcatgttag ctaagttcct gcttcgaaca cctgaaaatc agactgttgc tgtcggctta
     5521 caaacattta tttctgatgt gacaaaccaa agagtctctc tttttgctgc tggggcaatt
     5581 ttgattgcac taccgatttg tctattattc ttctttttac aaaagaactt tgttaccggc
     5641 ctgacatctg gcggaacaaa aggataggag cagagtatca tgcaaacatt tccgttaaac
     5701 tatttttccg cattacgtac gccaactcaa gtgtttgcgg gacgcaaact attatcatgg
     5761 cctaaatttt tcttgatatt tgtattttta gtttccttga tggtcatgcc tgtgactctt
     5821 ttttatgcca atcagatcca agcgattcca ttagaacaat ttctatccgt tcattcgctg
     5881 atcgatgaac aaggaaccca aaaattttct gaacttgaat tatctgaaac tgggttacaa
     5941 gcatcgcaac aaacgatagc tgtgacccct gaaattttgg taggtgtgtc tttatctgag
     6001 aaacaacaat ccgatcatgg aacttttatt gattttgaga aggaacaatg ggtgatccaa
     6061 caaaaggata aatctggtat acgccgctat acgatgaatt atagcccatc tttccaacca
     6121 gattctgtac gtacgcccga agatttccaa cgatttttag aaagagaatt ttatgccagt
     6181 aatcgtccaa caatcatttt atcttatagt ttaagtcttg gtcttgtcct atttgtcatg
     6241 acgagtctca ttttattcgg ggcttccttt ttcttgtgga tgacaagaaa aagccaactt
     6301 tcatccattc atacattcaa agagagtgca aatttgatgc tgaatgttat gggaataggt
     6361 tccatggcag cagcagttgt agggtttatc cactttgact ttatcttgat gctaagtgtc
     6421 caaacaacga taactgtctt gctgctttta tgggtttttg tcaaaacaag attcaaagat
     6481 aagcgtgtcg aatgacaagt ttaagcgatt tatgcgacaa tgaatgtgaa aaaaatgaaa
     6541 aataaagtag tttttgaaaa atttctgaaa aaattaaact tcttagttga ttcttttctt
     6601 ttttagtatt aacgtaatag ggtagttttt ttaaggagga aaatcatgtt agagaaaact
     6661 ttatacaatc aattattttc acgttctttc tcgctccctg tcgaagtaaa ttattgggac
     6721 ggtacaacga aacaatacgg agatacggac actcctcccc aaatcaaaat caatatccat
     6781 gaagaaatcc caatgaaaga gattacaagt aacgcttctt tggcactagg agaagcctat
     6841 atggatcatc gaatcgagat cgagggaagt atccaagcat tgatcaacga tgcttatcag
     6901 caagcaaaca gctttttacg aggaaaagat tatctaaaat ggctgccaaa gaaagaaaaa
     6961 catacgaaac aaaaaaacaa agaagatatc catgcgcatt atgacttagg taacgacttt
     7021 tataaacttt ggctagatcc aactttgact tattcttgtg cttatttcaa tacacctgaa
     7081 gatacgttgg aacaagcaca aatcaataaa gtccatcata tcttagacaa actctttatc
     7141 aaagaaggcg aaacgttgct tgatatcggt tgtggctggg gaacattgat gttcactgct
     7201 accaaagagt ataatgtcaa ggcaactggg atcacactaa gtgaagaaca atacgatttg
     7261 attaccaaaa gaataaaaga agaacattta gaagataaat gccgtgtatt attaatggat
     7321 taccgcgaac taaaaggcga aacgtttgat catatcacaa gtgtaggtat gttcgaacat
     7381 gtaggttctg aaaatcttga aggttatttc aaagtggtaa aagatttatt gaaaccaaaa
     7441 ggaacagctt tgatccatgg aatcagccga caacaaggcg gtgcgacaaa tgcttggatc
     7501 aataaatata ttttccctgg cggttatgtc cctggtgtta ctgaattagt tgataatatt
     7561 acaaaaaacg atttgcaact gattgatttg gaaagcttac gtcgtgacta ccaattgaca
     7621 ttagaacatt ggacaaagaa ttttcatcaa gtccaagatc aagtcacgaa agaaatgggt
     7681 gattccttct atcgaatgtg ggatctatat ttacaagctt gtgcagcttc atttgaatca
     7741 agtaatatcg atgtgattca atatttgctt gttcaacctg gaaacaatga catcccaatg
     7801 catcgtgcat aattgaggca aaagtactgt ttaaagacta tttaaaaaga agctaagttt
     7861 tatgatgctc cagaagtgaa cttttttgtc taacttctag gattgtctct aaagcttagt
     7921 ttctttttct ttatattttc ataataaaag tttggttctt taatttttaa acatgacaga
     7981 aaattttttc ttcgttatac ttgtaataga atactatttt ataacaaagg ggatcgaatc
     8041 aatgaaaaaa actgttattt atactagttt atttgcagct ttgtttttag ctgcttgtca
     8101 atcaaatacc aatcaaggca catctgggaa tacgtcttcc agtagcgaac agcgctcttc
     8161 acaggtatcg gactcctcaa caaaagcaag cacatcttcg tctagccaga caaaaagttc
     8221 tagtgaagca agtactcagt cagatatcaa agtgtctgca gctcgtgcga tcgaattgtt
     8281 ccaagataaa tacccagata ctgcaattac tagtttggaa ttagatagtg attggggatc
     8341 ttacttctac aagatagaag gtgtcgatga tcaaaaagaa tacgaagcaa gaattgatgc
     8401 tcaaaatgaa aaaatggaga cagagaatcc agaaactcta gatcgagatg aacaaaatgg
     8461 tcaaaagaaa aaagaagatg gtctggatgt tgataaattg atcacgattg aggaagcagc
     8521 aaaaatcgcc gtcgacaaag taggaagcgg tacggcaaca gattgggatt tggataaaga
     8581 tttaggtact acttattggg aagtcaaagt aaaagacggc aatcaaagta catcagtcaa
     8641 gatcaatagc caaacaggcg aagtactgtc aactgaacaa gatgattaag aatttcttcg
     8701 gaaaacaaga gttgattttt cttttagtca tgatacacta gacaaaataa cttgtttgaa
     8761 ggagcgattt acttgagttt agaaacgacc tttgccaatc atatctttgc caatcctttg
     8821 atgaatgcat ctggtgtcca ctgcatgact acccaggaat tagatgaact ggctcattca
     8881 gaagcaggag catttatcac taagagctgt acgatcaatg aacgaaaagg aaatcccgaa
     8941 ccacgctatt ttgatgttcc tttagggagt atcaattcta tgggacttcc aaacttaggt
     9001 ttttcttatt atctagagta tgctcttgcg tatgaaaaag tgcaggaaaa ccaaaatcaa
     9061 ccgttatttt tttccattgc cgggatgagt gtccaggaaa acttggaaat gcttgagaaa
     9121 atagaaaaaa gtgggtttaa tgggatcact gaattaaatt tatcttgtcc caatgtgcct
     9181 ggaaaaccac aactagctta cgattttgag gccacttatg aaacactgaa agaagtattc
     9241 agcatctttt ctaagccgct aggaatcaaa ttgcctcctt attttgattt tgctcatttt
     9301 gatcagatgg ctgatatctt gaatcagttt ccattgacct atgtcaatgc tatcaacagt
     9361 gtgggcaatg gtttgtatat cgatacagaa caagaagcag tagtcatcaa accaaaagaa
     9421 gggttcggcg gcattggcgg agagtatatc aagccgactg ctttggcgaa tgttcgcgct
     9481 ttttatacac gtttgaaacc agaaatccaa atcattggta caggtgggat tcggacagga
     9541 caagatgcat ttgaacatct gctatgtggc gcaagcatgt tacagattgg tacagagctt
     9601 cataaagaag ggccggaaat tttttctaga atcataaaag aattaacgca gattatgtca
     9661 gaaaaaggat atacgtcaat tgacgaattt aaaggcaaat tacgcaccat ttcatagcaa
     9721 agagacgaag gtcatgattg atcttcgtct ttttttataa aaaacactcg tctgttcatt
     9781 cctgtgcaag cgtactcatt gtatgttaga cgaacttgtg ggaaaataac agaggcaaaa
     9841 aggaggataa tatgaatgat gcaacagaat tagcaaaaga gcttaaagaa aaagaattta
     9901 gttttcaaga actagttacg gatatacaga aaaaaataaa gaagcaaaat catgaattga
     9961 atgcgtttgt cgcgcttgaa ccattcgaga gtaattccca cgagctattg attccccagt
    10021 cagaaaaagg gccattggct ggtatcccat ttcctttaaa aatgctcggt caagaaaaaa
    10081 agggatggct agctacttca ggatctcgtt tatttgaaac acaccgagct tctcatacat
    10141 ctaattatgt gcggcaagca gaagcgatag gtctagttcc ttttggacaa accaatgcgc
    10201 cagagtttgg tttcaaaaat attacagacc cagtcatcta tggcccagca agaaaccctt
    10261 ggaaccttga atgtacaccg ggcggatcaa gtggaggagc tgctgctgca gtttcatcag
    10321 gaatcgttcc tttagctggt gctagtgatg gcggtggttc tattagaata cccgcttcgt
    10381 tttgcggatt gattgggctg aaaccttcgc gagggacgat gccagttggt ccatatgctt
    10441 ggagaggatg gcaaggagct gctattgatt tcggcttgac agtttctatg agagacacgg
    10501 aggctttatt ttatgggatg agaagtatcc attccggtgc gccatatcaa gtatcgccag
    10561 ttgagtggca aacacatcca agaaaaaaac ggttgaagat agctttttgt gttgattcac
    10621 cgatccactc acagattagt gaagaggcag tacttgctgt aaaaaaagct gtagatttcc
    10681 ttggaaaaga agggcatgat ttgacagaga ttccttaccc actagatggt cgtaaactga
    10741 tcacgtctta ttatcagatg aatggcgcag aaacagcagc aatgatccgc tcgattgaaa
    10801 caggcatcca gcgagctgtc agaaaagaag aacttgaacc catttcttgg actttgttcc
    10861 aatatggtca gaagctctct gccgctactt acatcgattc tctgcatgta tgggatgaag
    10921 cagcaattgt catggaaaac ctacatcaag aatacgatct attcctttca ccaacgactg
    10981 catttaccgc ccctaaaatc agcgaagatt tgcaaagtga tgcgataaga gcacagatgg
    11041 aacacatcag tgattgttct gaagtagaag gtttagacgt tgtttcttcc atgtttgagg
    11101 agagcttgaa gatcacacct tatacacagt tggctaatct gactggacaa ccagcaatca
    11161 gcttgcctac tcatttgaca aaagaaaaca tgcctctagg aatccaattt atggctgcca
    11221 gaggaagaga agatttgcta tttcaaatcg gctatctatt tgaagcaaat catcagttcc
    11281 atttaccaaa tccatggaaa tagcagaaac aaggagaaga agaatgaaga aaaaaagatt
    11341 gtttgctcta ataactagtg ccttttttat tttaagcgct tgcggtacag ctgccgctaa
    11401 tcaggaagat aacagctgga gtaatgtaga aaaaaagaaa gaattagtag tagcgacttc
    11461 tggcacactg tttcccgcat cttattataa tgatcaaaat gaattagttg gttatgatgt
    11521 agatttggca aaagaggtgg ctaaacggtt aaatgtcaaa atctccttta aagaatacaa
    11581 cgtggatgga caaatcactt ctgtttcaaa aagagaatca gattttgctg cgaacgattt
    11641 tggtatcaca aaagaaagag aaaaaaaatt ctccctttca gagactttca aatattcatt
    11701 tgacagtatg atcgtacgaa aaaaagatga ttcaggaatc cactcacttg aagatcttaa
    11761 agggaaaaaa gcagctggcg aacccaatac tagctatatg cgtttagcgg aaaaacacgg
    11821 agctaaatta gtcacatatg acaatgccac gaatgatcag tatttaagcg atgtagcaaa
    11881 tggtcgaaca gatgttattc taaatgatta ttatctgcag aaaatggccg tatctgcttt
    11941 accagatatc cctgtaaaaa tactggagaa cgtttatttc aatgaaaacg aaagtggttt
    12001 tttattgaaa gaaggaaatg atgctctgaa aaagaaaatc gatggcgtat taaaagagat
    12061 gaaagaagat gggacgacaa agaaaatatc tgaaaaatat tttcaaactg atgtttcagt
    12121 caaaccgaaa gaaacgatgg ctgagtcggt gagtgagtga tgtactttcc tctaattgat
    12181 ttttccctca tgtggacttc acttcctttc gtactgcaag gcttggtata cacattaggg
    12241 attggcttcg tgtcgtttct tttaggaaat ttggtagggt tgcttttgac agttttaggt
    12301 cttttggatt ggctgcctct gaatgtattt attcgttttt atttatcgtt ctttagaggc
    12361 attcccgcac tagtcctgct gtttctgtta tatttcgggt taccttatca attaagtgcc
    12421 ttaacggctt cagtcatctg ctttacgatt accagcagtg catttattgg tgaaatctat
    12481 cgaggatcac tagcaggcgt ttcttcaggt cagtgggacg ctggtttagc ttctggtttt
    12541 tctttctttg gggtattgaa atatatcgtt ttgccgcaag cttttcgaat ctcagtaccg
    12601 gcactcagta acgtagctat ggatctagtg aaaggtactt cgttagcagc aacaatcaca
    12661 gtgccagaaa tatttcaaaa agcaaagatc ataggcggtc ggacattcga ttatttttct
    12721 ctctatattt tagttgcact gatttattgg gtcatttgta tcgggatcga aaaagtgcaa
    12781 cagtatttag aaaaacgttc tatccatcag tcaatctgaa aaatagaaat ggtgctagcg
    12841 tggctggcac catttctatt ttttgtttct tgtatattct gcctagtatt ttaaaaccat
    12901 ttcatgtaaa atgtaaatcg aaaatatgtt cggaggtaga aaacatgaag accccgctaa
    12961 ccatcgaaat agaatcctcg ttataccaat attgcatcga gcaaggcggc ttggttgtag
    13021 aagaagtcac gatgcctgat gaccaaggaa ttgtagatac gcttagctgc ttgacaaaat
    13081 ttgatggaag ccgagaatgg cgatgctatg agctgaaagt gacaaaagca gattttcgct
    13141 ctacagccaa actgtcattt gttggacatt ataattattt tgttttacca agtaaactat
    13201 atgaagaagt aaaaactgaa atccctacag gaataggtgt tttagtgtat cgtccgtaca
    13261 cggttgaaga ggagatgcct gcctctggta catttctgat tgcgaaaaaa gcagcaaaga
    13321 aagagcttgc tgtaccggaa gaagctttga caaaccgctt catggcttct ttattcaggg
    13381 aagtaagaaa agcaaagcaa atggagcgag gcgtaaaata ttttccaaaa gatcaattgt
    13441 acaaagaatt gaaaagacgt tcagaaaatc aagatccatt ttctaacgaa cgatattatg
    13501 aacggttttt agaggaaata gctagtcagg caatcaatga gttgcaagaa gaagttatcg
    13561 cgcttcagca tgattatgaa tatttgaggc aacaacagca gatccgtcgg ttgcctacag
    13621 agccattgga gtgaaaaata tgtactatcc ttatttaaga ggaaaacaat ttgacctatt
    13681 agcattaaaa gaagcactgt cgcgaggatt gctctccaat aaaatccaac cagtgatcga
    13741 accagtacgt gattccgcta cgcttaaaaa tgtgatcgaa ttatttcaaa aaaagcacca
    13801 tccaatcgct gtgatccaaa atccccaagt aggacaattc aaattattcg atcagcatgt
    13861 gcacagttgg acagtcaaag aaaatagctc tgtcgtccct gcacagatct taacgccaga
    13921 aaatctcagt gaagtactgg attcacctcc agcgtttctt gttttcgatg gacagcacta
    13981 cccaaaagat acagaggtgt ggaaacagtt ggcaggagtt gattcgaaat ttttgatacc
    14041 agatactagt cgctttcgca tctggctgcc cgaaaacaaa attgtgatcc gtgactcttt
    14101 tcaaacgaga aagcatgttg aaagttatgc agataaaaca gatgactttt ttagtgatga
    14161 ctatcttttt ttccacgcag acggatatat tggattttct gatttcacga tcgaaggtag
    14221 ccgttatttc gataaagggt tccctagccg agcgcttgct atccatctaa cgtatattga
    14281 tgcttacggc aatatacgcg tgaaacattt tgtctctgac agtaacgact ctgctaaaga
    14341 ccaagcattg aagtttcttg aagcaggaga taaaatgaaa aaatggatca tgcgtcacca
    14401 tcatcaactc ttgattactt caggaatgat tgaattattg gctctttacc agcaacaaaa
    14461 attccctgga ctgggagtgt tgaaaaagtg gtcacttatg catcatttgg aacttattag
    14521 tcacctgcta gatcatccaa aagattggct taaatattat tcaaaagttc aggaactata
    14581 tgaagtgatc caatgattag ataatgtaaa tgaaaaagga atagaaaaga ggaaactgga
    14641 tgtatattcg gcaaacaagg aaagaagaag tagaagaagt ggtagaaatc attaatgccg
    14701 cagcggattt cattgcttcg caaggctctc ctcaatggca aaacgggcag aaaccaacaa
    14761 aagaaacgat tttgaacgat attgaaagaa aagaaagcta tgtattaata gtggaagatc
    14821 aaattgctgg aacagctgca ttagtctcag gcgtggatcc cgtttacaca aaaatagatg
    14881 gtagttggaa agaatcaact ggtccttatt tatcgattca tcgtgtcgct ttagcagaaa
    14941 gtactcgagg aaaaaaacta gcaaaaccgt tcttagaaca tttgttgacg attgcagcca
    15001 gcaaaaatat ccaagatatc cgtatcgata cttatccagc taatgaaccg atggaaaaga
    15061 ccatctattc tgctggattc acttatcaag ggatgattga atttcctttt gaacacggcg
    15121 aaagaaaagc atatcaaata atcatcaatt gactataaaa tcatctgagc aaagataaag
    15181 acattaacac tactgaaaaa aacaatcata tgatagccaa gaggttgtaa cagaagtggc
    15241 cagcttcaaa aaacaagaaa gaatttccga aaatgcttct caaatttttg tggaatttta
    15301 gcgtatttta cgaaatagct gctttctgaa actcccttca tcgagaggtc gtgacatagc
    15361 ttttgtcacg atctctcggc ttatcctacg agtattattc gatacattta tatcaataat
    15421 ccttttttta ttttatctgc agcttcttgg aacgctccat cgtttgacac gactaaaagt
    15481 gaggggatag ctttcaaacg tttcaactgt gctaaatctt ctacataaac cgccagtctg
    15541 gctgaaatct gttcagatga atctccacgt tttttcaggc gcgattgcaa tgtttcttta
    15601 gagatcgtaa taaagatagg acggactacc gtcccgaaat actcatataa atgccaaaaa
    15661 ccatttgctg tcaaaacggc gtaacaattt cctactgtca gtttcttttt aatttctgct
    15721 ttactggaac catatttatg ttgagcatat tggtcccact ctgcaaactc atttcccttg
    15781 atcatctgat tgaatgtatt gtcagaaaca aaataataat cctcttgatc tttttcccca
    15841 ggtcttggtg atcgattcgt gtaagtaatg atcttttgtt ccggctgaaa aatagcttct
    15901 gcgagtgaag tttttccaga accagaagga ccgataaata catagcaata attcattgtc
    15961 atttctcctt tgatgattga acaagtgaaa aagctttgga aaatatttcc aaagctttta
    16021 aaagtaactt tgcttttttg tattttatac cagcaagcca gtcacaatag cacttaaaaa
    16081 gctgactaaa gtcgctgtta gcaaaatttt caatccgtgt tttgcgacaa gatttccttt
    16141 ttcttcattt aatcccttca tcgcaccaga aatgatcccg atagaagaga agttggcaaa
    16201 tgaaacgagg aagacagata taattgctgt tgtacgctca gtaaaatgga aattaccatt
    16261 tgctaattct gtcatagcaa caaattcatt tgtaaccagt ttagtcgcca tgatactacc
    16321 ggcatccact gcttctttcc atgggattcc gctgatgaag gcaagagggg caaagatgta
    16381 acctaggatc tcttggaaag tgatgccgaa tatcccgtgg aagatagcat tgatcatggc
    16441 aatcaaagca acaaaaccga taagcatagc agcaaccgtg atcgccacat ggaaaccatc
    16501 aagaatgtat tctcccaaca tttggaaaaa tgtctgttct ttgttttctt caatgatcag
    16561 ctcatcttct ttttcttcta atacataggg atttacaatt gaagccagaa tataaccgcc
    16621 aaataagttc aaaacaaggg cagtgatcac atatttagaa tcgatcagtg ccatataaga
    16681 tccgacgatc gacatagaaa ctgtagacat agccgataca ctcatggtga acaaacgttt
    16741 ctcagatagg aagggtaatt ccttttttat ggaaataaaa acttccgatt gtcctaaaat
    16801 agcagaagct actccattat aagattccaa tttgcccatc ccgttgattt tacttaatcc
    16861 tagaccaaca gctttcataa ataatggaag aatcttgata tagcgtagaa taccgataat
    16921 agcagaaata aatacaattg gcatcaatac ggaaagaaag aaaggtgcac ttcctttatt
    16981 tgctacaccg gcaaaaacaa agtcaacacc ttgcccagca aaagcaagaa gatgatcaaa
    17041 catttttgcc aatacagaaa caaccgctgt accaaaagtt gtacgtaata atacgaaacc
    17101 aagaacaagc tgtaaaacga acatcacaat tagtggtttc aatttgattg cttttttgtt
    17161 actgctgatc aaaaaagcaa gccctaatgt gacaaataaa ccaataatac ctaataaata
    17221 actcaaataa tcactcctat tccaaattcc gttttatttt acctgttttg caaataaaat
    17281 actaatcttt agtgaaattt tcattacttt tttagagtga aacgcattca ttttccatta
    17341 gaaaataaag cgaacaaaat aaaaggttta aaaagtgtaa cgtcttagta aagtctataa
    17401 aaaaacgaaa ctatcgatta agctaatagt ttcgttttca acatttataa gtaaactttt
    17461 tctattggta atatagattt tcggaagggt agacaggtgc gcaggtaata tcgattccta
    17521 gttttttcaa tgtttgttca tcatttttat tgaccatcac agtagagtgg gcttgtgctc
    17581 cctcaagttc agctagttta tcataagcaa cttgagcgat tggattcgtt actgcactga
    17641 ttgataaagc gatcaagact tcattgatgc ttagtgcatc caaagaggaa tgaagtgtct
    17701 ttgatttcaa tccttgtatt gtttcaagaa ctaatggtga caataaagga atgtcatctg
    17761 cgatatgggc taaatgtttt actgcattta agataactgc cgctgacgca tccatcaatg
    17821 aacttgtacg cccggtaatg atgacttcat ctggtaattc gattgcaatc acagcagaag
    17881 gatcttttgt aggactttgc gcttgaatct tatctgcata gtcatgggca gggactacca
    17941 cgtttcggtc ttcaggacgt aaaccgactt cttccataat cagtttgatt cgattaactg
    18001 cttcttcgtc gatcaatcct tttttgaagt cgcattctgt ttggaaataa cgacggatga
    18061 tttcttgttt agaggcttct tggacaacgg tatcatccac gatcccaaat cctacacgat
    18121 tcacacccat gtcagtagga gattgataaa cagattcttt accagtaatt ttttcaataa
    18181 tacgtttgat tacaggaaat gtctcaacat cccgattata gttcacagct actttattgt
    18241 aggcatcaaa gtggaaggaa tcgatcatat tgacatcttt tagatcgact gtggctgctt
    18301 cataagcaat gttcagcgga tgtttcaacg gaacattcca tactgggaaa gtttcgaact
    18361 tggagtaacc agctgcatgt cctttttggc tttcatgata cagttggttc aagcaagtag
    18421 cgagctttcc acttcctggg ccaggagctg tcactacaac gattggttta gttgtttcaa
    18481 tatactcatt ttttccaaaa ccatcttctg agacgatttt ttctacgttg actggatagt
    18541 cttcgatttc tgagtgttta tagaccttga tgtttcgtct ttctagttta ttgataaaga
    18601 ctttcgtggc aggctgtcca ctatagcgag tgataacgac actgttgatt aataagccat
    18661 aaccacgtaa ttcatcgatt aaccgcaaga tatccatatc ataagtaatc ccataatcac
    18721 cacggatctt attacgttca atatctccag catagacgca aatcagtatt tcagcttgat
    18781 ctttcaattt ttgcagcaat ttgattttcg catcttcttc aaatcctgga agtacacgtt
    18841 tcgcatgctt gtcgtcaact aacttcccac caaattctaa atataatttg tcatagtggt
    18901 tcactcgttc caaaatatac gctgactgct cttcaatata tttttctgaa tcaaaaccaa
    18961 tttttttcac taggttccct ccttaagtac taaagcattt tttcattttt ttgaggattc
    19021 gtcaactgga agagcgaaat tcctcaaaaa aacaaagaaa atggtttaaa ccaacttgtc
    19081 tagatagtta ttcattggta tgatagtttt tgataaggaa ggtgctacat gaaaaaaaga
    19141 cagaaaaaga aaaacgcata taaacactat atccgttcga tttttaccgg ttatgaaaga
    19201 atgcttgaag atcccgaatt ggaacaattg acgtttactt atctaaacga agaaacgcag
    19261 ctaactcgtg atgagcatca gcgcattcat ttcacaacaa gagatctgcc tagtaaataa
    19321 aaaataagat aaaggtcatc ggattcgatt ctgtcctatc agatggaaag actagttgat
    19381 cgaaaaaaag gctgtaccaa attttggtgc agcctgtttt atggagcatt gcccgctctt
    19441 ttttataaca attttttaca gaaaatggta ctattgaagt attaataagt gaaaaacaga
    19501 tgagtaagag gaggcaagta gtatgaaaga gccagataca aatgcaaatt atgagccaga
    19561 cgaactagag gagacgaacg cggatcagga tacaggacaa gaatcgtctt ctttttacga
    19621 tatcaaagaa aaagcaagca tgaatctttc ccaattatta gaagacttac aggcttttga
    19681 aaaagctgtc cgcgaagaag atatccctga gatctaccgt ttatacaatg gccgattgaa
    19741 tgaagaatta aaaagaagct caaatgaaaa tcacgagatc gatcaattgc tgatgcgtaa
    19801 gatccatgat cgttttttac aggaatttcc ttttatggaa caagtagaaa acatctcgcc
    19861 aacgatgagc tattacaaaa tcggcactta ttaccatgat cggcccacgg tcggtattga
    19921 tgcaagtctg ccagaaattt ttgttttgcc taagatcgat gaagagtggg agaaatattc
    19981 ccaaccggag tcaacggact atgacaaaaa aatcaatgag ctagacgcaa aggtgatcac
    20041 tgctcagaca gaaattgaac gtttagatga acaaataaaa gaaatcaatc gacaaatgac
    20101 tgatttgaac gacaataaag gattcttgaa tcgtaaaaaa atcgaggaag aaattcaaga
    20161 attagagaag aagaagcaag tactatctaa tgaaaaatta ggctggcttc cttatattga
    20221 gacgcctgaa acaatccagc agcaaaaaga agcattgaag caagaagcaa gagcagacca
    20281 gcttcgtgca gccatcgtag aaaaagaaca aaggcaaatc agacgctatt tcggtagcaa
    20341 agagggattc ggtcaagcaa tccatgaatt cttgatgaat tatcttggca atgagaatcc
    20401 taataatcaa tctaatgaag gaggttccga gtatgaatga acacgatgaa gcagaatgga
    20461 caaaaaatga agaacaaaca gaagatcatg aagaaacgaa cacttcattg gaagaacaaa
    20521 caagtaatcc tctggatgac acagaagaaa tccctatgct agaggaaaca aaaatacatg
    20581 aaaaagaaaa agtaactccg acgaatacga atactgcaga aaacacccat gcttacgaaa
    20641 taactgaaaa tcccgggtat ttagaaggaa aagagcaaga tcatcaagat atcgtcagtt
    20701 tgattgaaac agcggaagca gaattagcgg atttacgtat gagaaaagat ttgatcgaat
    20761 cagattttcc acaattatta gaatcatatg atcgttttat attgaatgaa gaaccaatct
    20821 ccacgatcgg aaagacaagc ctgatcgaat acttcacgta tgaattactg aaaccgttga
    20881 atgatattgg tctggaacgg tcaaattcag cttatgacac atggaaaaca agacattttt
    20941 cgatcaatta tacgttgaat ggacaaaacg aattagtctt ttcctttgta ttaccgacca
    21001 tcgatcaacg ataccaagtg gaatcgatga gtctcctaaa cgtatcacca gagacaatgg
    21061 aaatcaatgt ccaagatgac cgggtgcttt ctttgatccg acactggtca gtggatcgag
    21121 tattctctgc agggcagatc acgatattca atcacaaatt gaatcaatta ttagcccatg
    21181 caagaacact tggttttacc gttaatcaga cattactaga caatacgaag ccacttcgtt
    21241 tgaatcttca gagcgaattt gaattgacga atgaagtatt ggatgatatt tttatcgtgg
    21301 caatgaaaaa tccatcctac gatatggaaa agatcaacga aactacttat caagtacgat
    21361 tagataaagg acagtcttta acgataacaa caaacgaaaa agatcaaaca gtcttgtcga
    21421 tctcatctgg agactaccca cgatctgtca ttgatttctt tatcaattac gaattcttgg
    21481 tcccattaat ggtgagaaag tcctgattgg ataagttgga ttaccagatg tcattcagat
    21541 aattctgttc caaaggactt gcggcagtta tccagaataa ttgtcacaag tcttttctgt
    21601 atgtactaaa tggacgtgaa taacttgctg atgaaggagg gatttcatta ggaatgtcca
    21661 caaataaaat agcacagctt agcttattgt ctgctgcttg tgtagcaggt aggatcgctt
    21721 ttcaatttat tccgaatttc cagccaatga ctgctatttt tttatttata gtactttact
    21781 taagcttaaa agatgcgtta attgtcatga gtctttctat tgcgatcagc agtttttatt
    21841 ttggtgttgg accatgggtc atcggccaat ggatcagcta tactgttgtt ctgagtttgt
    21901 tttcaatcgt ctgcttgaga aaaacagtgc aaaggaatct atggttaaag ggagtctgtt
    21961 tttttctggc gggcatggtt tacggaatag gtatgaccgt ttttgatacg ctgttatatc
    22021 gtttgcctca accttggatc tattaccttc aaggggtgtc gtttgatttg atgcacagta
    22081 taggcaatgt tgtgttcttt cttgtttttc tgccagtcgt caaacgtttt tacaatcatt
    22141 caggaggaaa aaatgaaaaa aataatttat agtttgcttg caatgagtgt cagtatactc
    22201 attttgacaa gttgcagtac aacctataca caatcggctt ccaaccaaaa ggaggaagta
    22261 aaaacatcgc aaaaagcaac aatcacgtta caagaaaatg gtcaaaactt ttcggaaaaa
    22321 gaagtggtct tcaaaaaagg cgatgattta ctgagtattt tgaaacgaaa ttttgaagtc
    22381 aaagaagata acggcttcat cacttcgctt gaagggcact cacaggatga gaaaaatcaa
    22441 ctctattgga tgtttactgt ggacgggaaa agtgctacta ctggtgccaa agagatcaaa
    22501 ttgaatgatg gacaatcggt cattttcaat ttgtctaaac tataagtttc ataaaaaact
    22561 gttaaagggc gaatattgcc ataattcagt gagttcagtt gatgaaagaa ggaaaataac
    22621 atggaaaatt ttaaaaaaag cggaatgttt tccgtaattg ctgctctagg ggctaatatt
    22681 ctagttgcta tcagcaaatt tgtcggctat gcaatcagtg gcagtgccgc catgctaaac
    22741 gaaagcatcc acagtatcgt tgattgcagc aatcagattt tgctgttatt cggtgataaa
    22801 caagccaata aaggacagag cgagttgcat caatttggtg aaggacgagc aaaatacttt
    22861 ttcagtacaa tcgtcgcaat gatgcttttc ttcggcggtg gggcacttgg ggtgatggaa
    22921 gcttttgaaa agttgacgca tccatcccat gaagtgggta atccgatcat tgttatagca
    22981 atactgttat ttggtttggt aatcgaagga agttcgttac gcgtggcaat gaaagaaatc
    23041 aaagaattga atactgaaaa actttccacg atgcgctttt tgcgcgaaag tcgacacagt
    23101 gaaatactga ttatttttac ggaagacttg tgtgctgtaa tcggtttggt cttggcttta
    23161 gcaggaacaa tattgactta tgcaaccggg attgctgcat ttgatgcgat cagcggattg
    23221 ctgatcggtt tcttgctgat gggagccgcc attttcctag caaaagaatt ttatagtctg
    23281 atcatcggtg aaagcgtgac agcaagcgat ttatcaaaaa tcaagttagc ttttgaacgt
    23341 cctgagatca gtcggctgat cgatataaaa acagttcatt taagtccaac ggagatttta
    23401 gttgcagcaa aagcagatat cgttggtaac aaagagaatg aagcttactc ggtcatcaat
    23461 gatattgaaa aaaatatgcg tcagtctctt cctgacaaga agatgtatat atacattgaa
    23521 acagataaat atgacccaaa ttattctcgt aaataaaacg tttattcatg aattgaagca
    23581 aaaatgagaa aaatataatc ttgtttttaa ataaaacggc tacaatttag cgaatttatg
    23641 ttacaatgag tccgctgttt atttgttgta attaaaggag atattttaat gaacaaaaag
    23701 aaaagtgcgc catggcttca agctttgcat gcagtgatgc cactttgtat aagctatata
    23761 ccagtaggat tagcttgcgg agtattgttg caaaaagttg gatttgatcc tctcttatca
    23821 ggactgttat cgattctggt cttttccggt ggcgcgcagt ttttagttgc atctatgctt
    23881 acgacgcaag catcatttgc aacgacatta ttgatggttt tctttttaga attacgctat
    23941 acattattag gagcaagtct tgccggtttt atgaaaaaag aaaagcggac gtttttggct
    24001 gtcttttctc aatcactgaa tgatgaaaac tatgcggtga actacttaaa gttttcaact
    24061 gacccttcct ggaataaaag aaaggcttta tatgtcaatt ggttctcaat gactgcatgg
    24121 gccggtagca atatgatcgg taattttttc ggctcggtga ttcgagtaga tgctgacctt
    24181 gtccattttg ctttgacggc catgttcatt ttcatgttta tcatgcagat gaaaagcgca
    24241 ctactgatct ttacaggttt attctcaggc gtattagggg tcgtattcat ggtattattc
    24301 caaaatactt tcggattgat tgcagcaaca gtcattgctt catttgccgg atttatgctg
    24361 gagaaggcat tcaaaaaaga aaaagctaag aaactaagag accgcggtaa agatgtcaat
    24421 gagccgctct tcaacttccc ggaacaggag gccaatcacc atgactaaac tatatttgct
    24481 acttttagtt gcttgtctgt tcatcgtggc atatatcccg cgcttgttcc cgatgcttta
    24541 ttttactcat cgaaaagtgc catcctggtt cagtgactgg atgaaatatg taccggtggc
    24601 attatttgcg gcgttagctt ttaaagatgt tttcattact catgaacatc tggatattgc
    24661 ttggaatatc aaaatagctg caatgattct agtagcaggc gtggcttaca aaacacgttc
    24721 gatggcgttg tcagttttga caggacttgc gtctgtcttt ttgctgtcta tgctttaaac
    24781 tgttttattt tattaaaaac agagattgcg acataagttg ttcaaactga tctatccgaa
    24841 ctgcatttgc aacagtcaat tctggatgca agtttattcg tagggataaa aaagattagt
    24901 gttggcttat gatgcaagct cttttttatt ttaggaatta gagagggatt tgtccttcta
    24961 tgattttttg ctataatagg gaagatgttt attttatgaa agaatagttg agggaattta
    25021 aatgaataaa aaattgatgc aaggaacatt ttggctaacc tttgctaatc ttctatgtaa
    25081 agttttaggg gtagtctatt tgattccctg gctaagtatg atggggaaca atcaagatgg
    25141 tatgcttgct acgacattgt ataatgttgg ctatcttcct tatggattgt tcctgatgct
    25201 tggtacagtc ggatttccaa atgcgattgc aaaaaaagta gctgtagcca caaaagaagg
    25261 agataacgct gcttgtcggc tgatttttag aagtacgatc aatatcatgt ttgtcatcgg
    25321 tatcgcctct gctgcactga tgtatttatt tgctccttta ctagccggaa tcagtccgat
    25381 tgctaacgtt aataatggga tactagctat cagaagttta tgcccatctt tgattgctat
    25441 cccaattctt agtgcaatga gaggatattt ccaaggaaaa aatgatctgc ggccttatgg
    25501 aacttctttg attatcgaac aggctgtgcg agtgatcgtt atcttggctg gtacttatta
    25561 cttgcgcgta ttgactgacg gaaccatatt agaagctgta ttgatcagta cagtcgcttc
    25621 tttcttcggt gggttagctg cgattatcca tatgtatatc gtcggacgca gaaaagactt
    25681 cttcgaactt aaagattttt tgatttccag ccgttatttc gaaagagaaa accgatctgc
    25741 ctctgtctct atcattcggg aaacactacc gtttattttt gtcggctcag tcattactat
    25801 cgttcaaatg atcgaccaag taacgatgaa accactgctt cactttttcc gtccagagat
    25861 cgcagatcag caactggaat tcttattcag ccgtgcttct gtcaatccaa ataaattgac
    25921 attgatcctg atctccatgg taggaacagt agctataagc agcttgccaa tattaagcac
    25981 gatgaagaaa aaagatcgga tacaaatcga aaaaacagta ggagatagtt tttcgattgc
    26041 gttattgatt cttcttcctt cattgactgg aatgtcttta ttagctggac cactatacac
    26101 attgtttttt ggctatgatc ctgaaagtgt cggatatttc caaatggctt tgctagcttc
    26161 tttgtttttc tctttattca cgatcctatc tacgatgctt caatctttga atcaccatcg
    26221 ttttgcgatc cgattaactg tagaagcaat tatcctgaaa gtcatcttcc aagtaatcgg
    26281 attaggatta gttggcggat acgggatgag cttatcaaat ggcttagcat tcggtattgt
    26341 ctttgttcgc ggctatcact ttatgtgcaa agaataccga atagcacctt tagcaaagat
    26401 cagcaatttt ttcttaaaaa ctttccgctc caccctgatt atgcttgcag tatgttttgc
    26461 cgttttcttt ttgctgaatc agcgattgtc aatggaatca aaaagctatg caatgattta
    26521 ttgtgtggct gtaggaagta ttggcggctt agtattttta ttttctcaat tcggaaaaaa
    26581 tgggtttcga atgttgaaaa atgttaaaca tcatagcaga agcaaagaat aggttaacta
    26641 atcgtttttt gctgtcactt gaaaaacggc aactcgtatg attacgcttg tcgtttttct
    26701 attttccaat tttagtttca tatctaatct tacttatgat atagttataa ggtctggaaa
    26761 aattctttga aacgaggaaa ttaaacacat gaaacttgca gtagttacag atagttcggc
    26821 atatctgccg gaacgtatca agaaccatcc cgacttattc gttattccaa tccctttgat
    26881 tatggatggg aaaatttata acgaaggaat cgatatcgaa gcggatgaat attatggact
    26941 tttaaaaaac agtaaagatt ttcctactac ttcacaacct gtcgtagggg aagttttggg
    27001 tttatatgag gaattgaaag agaaagggta tgacacgatt ttaagtatcc atctttcttc
    27061 agggatttct ggctttgtca acacattatt tgcgatgaaa gatgacatca gcggagtaac
    27121 ggtcattcct tatgactcta agatcactag tatgccgatg ggacacatgg tagaagctgc
    27181 cttagactta aatgatgaag gtcagtccct tgatgaaata cttgcacata tcgatcgaat
    27241 ccgagataat acctatgctt atctgattgt agatgatttg aacaatttag tacgaggagg
    27301 acgtttgaca aatggtgcag cgctgattgg cagcttatta aaaatcaaac cgatcctgac
    27361 attttctgaa gggaaaattg ttttattcga aaaaatccgt tcaagtaaaa aagcatttgc
    27421 tcgagctgaa gaaattattg gtagaagaaa taacgaaata caaccgccag tcaaacttta
    27481 cgtgatacat gcaaataatt tagaagtagc gattgcggag aaagagaaat tgcagaaaaa
    27541 atatccaaat gcagaaatcg aaattggtca tttcggacca gtcattggta cacatttagg
    27601 tgaaaaagcc atcggcattg ccatttcagc gcaataattc cttttattca aaaaacatac
    27661 caaaaatat
//
LOCUS       DACCYH010000031        26768 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.031, whole genome shotgun sequence.
ACCESSION   DACCYH010000031 DACCYH010000000
VERSION     DACCYH010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.031"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(26..1957)
                     /locus_tag="IVY85_001282"
     CDS             complement(26..1957)
                     /locus_tag="IVY85_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8044309.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1975..2889)
                     /gene="pfkB"
                     /locus_tag="IVY85_001283"
     CDS             complement(1975..2889)
                     /gene="pfkB"
                     /locus_tag="IVY85_001283"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP8044310.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2886..3638)
                     /locus_tag="IVY85_001284"
     CDS             complement(2886..3638)
                     /locus_tag="IVY85_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8044311.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3797..4006)
                     /locus_tag="IVY85_001285"
     CDS             complement(3797..4006)
                     /locus_tag="IVY85_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044312.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4017..5344)
                     /locus_tag="IVY85_001286"
                     /pseudo
     CDS             complement(4017..5344)
                     /locus_tag="IVY85_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5709..6296)
                     /locus_tag="IVY85_001287"
     CDS             complement(5709..6296)
                     /locus_tag="IVY85_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP8044313.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6379..7629)
                     /gene="clpX"
                     /locus_tag="IVY85_001288"
     CDS             complement(6379..7629)
                     /gene="clpX"
                     /locus_tag="IVY85_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP8044314.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7876..9165)
                     /locus_tag="IVY85_001289"
     CDS             complement(7876..9165)
                     /locus_tag="IVY85_001289"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP8044315.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9319..10251)
                     /locus_tag="IVY85_001290"
     CDS             complement(9319..10251)
                     /locus_tag="IVY85_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044316.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10408..11067)
                     /locus_tag="IVY85_001291"
     CDS             complement(10408..11067)
                     /locus_tag="IVY85_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP8044317.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11070..12095)
                     /gene="sppA"
                     /locus_tag="IVY85_001292"
     CDS             complement(11070..12095)
                     /gene="sppA"
                     /locus_tag="IVY85_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP8044318.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12108..12398)
                     /locus_tag="IVY85_001293"
     CDS             complement(12108..12398)
                     /locus_tag="IVY85_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044319.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12807..14612
                     /gene="glmS"
                     /locus_tag="IVY85_001294"
     CDS             12807..14612
                     /gene="glmS"
                     /locus_tag="IVY85_001294"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP8044320.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(14684..16039)
                     /locus_tag="IVY85_001295"
     CDS             complement(14684..16039)
                     /locus_tag="IVY85_001295"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP8044321.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16063..17232)
                     /locus_tag="IVY85_001296"
     CDS             complement(16063..17232)
                     /locus_tag="IVY85_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044322.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17229..18116)
                     /locus_tag="IVY85_001297"
     CDS             complement(17229..18116)
                     /locus_tag="IVY85_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP8044323.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18305..21997)
                     /gene="nifJ"
                     /locus_tag="IVY85_001298"
     CDS             complement(18305..21997)
                     /gene="nifJ"
                     /locus_tag="IVY85_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP8044324.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            22196..22717
                     /locus_tag="IVY85_001299"
     CDS             22196..22717
                     /locus_tag="IVY85_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8044325.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(23416..23973)
                     /locus_tag="IVY85_001300"
     CDS             complement(23416..23973)
                     /locus_tag="IVY85_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044326.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(24152..24763)
                     /locus_tag="IVY85_001301"
     CDS             complement(24152..24763)
                     /locus_tag="IVY85_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044327.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(24876..25133)
                     /locus_tag="IVY85_001302"
     CDS             complement(24876..25133)
                     /locus_tag="IVY85_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044328.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(25730..26008)
                     /locus_tag="IVY85_001303"
     CDS             complement(25730..26008)
                     /locus_tag="IVY85_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044329.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(26042..26293)
                     /locus_tag="IVY85_001304"
     CDS             complement(26042..26293)
                     /locus_tag="IVY85_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044330.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         7558 a         5767 c         4584 g         8859 t
ORIGIN      
        1 aaacccagcc tcttcttttt tatttctatg cttctgctac tgcaggagct tttctcaata
       61 aaccataaat cacaccagat acgactgctc cgatagcaat aaacaccaaa tacatgaccg
      121 ggtgactcag taagaataca acgaaaatcc cgccgtgagg agccagtaat ttgattccga
      181 aagctccgac taatccgcca gttaaagcag aaccaataat aaaacttgga atggcacgaa
      241 tcgggtcagc tgctgcaaat ggaatcgatc cttccgtgac aaacgatagt cccatcacga
      301 tgttcgtcaa accagcatct tgatctgttt tgctgaattt attcttaaac aaacgtgtag
      361 caacgaaaat tgccaatgga ggtaccattc ctccagccat aacagaagcc atcacgacac
      421 ttccgccagt tgcaacagtc gtagctaaag ttgctgtacc aaacacataa gcggctttgt
      481 tgattggacc gcctaaatct gccgccatca ttccagctaa taatgcacca agtaaagcag
      541 cattcgaacc actcatactt gtcaagaaac cgttcaatgc atcattgatt gctttcatag
      601 ggacattgac cagcaacatc aagaaaccag taatcagtaa gccaaacact ggataaaaga
      661 ggatcgtttt aattccatcc aatgattttg gtaagttctt gaataattta cgtaaaaata
      721 cgatgacata accagctaag aatccaccaa tcagtgcacc taagaatcct gcaccgcctg
      781 tattagctaa tgcaccagca gcaaatccaa cgatcaaacc agggcggtca ccgatacttg
      841 aagcaataaa tcctgctaat accggcagca tgaaaccaaa tgcagcttgt ccgatttggt
      901 tgaaccagct agcagcttga ttgtaattcc ctagatttga taattgatca tgtggcacgc
      961 caataaattg gtcgatcatg aaagaaagcg caatagcgat tcctccacca ataacgaaag
     1021 gtaacatgtg tgatacgccg ttcatcaaat ctttgtaaat tctttgtccg atagtaccat
     1081 ccgcatttcc ttcttctgaa ctgctttctt ttccatcacc atggaatact ggtgcagtac
     1141 cactaagggc tagattgatc aactcttctg gtttacggat accatcactg acaggacgat
     1201 tgatcagttc ttttccgtca aagcgattca tctctacttt tttgtctgct gcaacgatta
     1261 caccttctgc acgggcgatg tcttcagcag tcaatcggtt tttgattcct tctgaaccgt
     1321 tcgtttcgac tttgatctcc acacccattt ctttcgcttt tttcttcaaa gcatcttctg
     1381 ccatgtaagt gtgagcgatc cctgttggac aagctgtaac ggcaacaacg aattttcttt
     1441 ctggatgagc agaagcttct gcagcttgtg tttcttgttc tgctttttct tctgcttctt
     1501 tttgctcttc agctgtttgg aacaagtctt ggacttcttc tggtgtattt gcttctttta
     1561 attttcctac gaaatctgca tcaatcagca atcgagatag tgctgctaat gcttgcaaat
     1621 gtgtgtcgtt tgcgccttct ggagctgcga tcatgaaaaa cagataagtt ggctgtccgt
     1681 ctaaggcttc ataatctact cctttttggc tttttgcgaa taaaactgtt gcttctttaa
     1741 ctgcactgtt tttcgcgtga ggcatggcga ttccatcacc aagacctgta gaagtttgtg
     1801 cttcgcgggc tagaattcct tctttatatg tttcgatatc ggaaatccgg ccggcatcaa
     1861 acattttttg gaccatttca tcgattgcgc cttttttatc agtcgcttgc aaatccatga
     1921 tcatcgcttc tttgaccatt aaatccttga tattcatgat tatcctcctt gaaactaatc
     1981 aatttttgtg atttcaactt ctggcagcaa ctcatcaatc aattcgcgag ctgccaagtc
     2041 atcagaaaaa gctgttgcgc ttccgcaagc aactccccat ttaaaggctt cgattggatc
     2101 tttcgtacgg ctgtaagccc caataaagcc agcaatcatt gaatcccctg cacctactga
     2161 atttttcaat gggcgaacta atacattcgt tttataagtt ccttgttccg tgaagagcaa
     2221 agcaccgtct ccagccattg agatcagcac attctgtgcc ccttcagcta gtaatttttt
     2281 cccatacgga atgatttctt ctacagattg gaaggttgtg ttgtaaagct cagctaattc
     2341 gtgattgttt ggtttgatca acaacggttt cttaacaagt gcatttttta ggtcatcacc
     2401 tgttgtatca atcacgaaat ctgcaccttt ttctcgaatg atatcaatca gttcttgata
     2461 aaatcctttt cgcaggctag ctggcgcgct tcctgacaat acgacgatat ctccttgtcc
     2521 tactgttcct aatgcttgct tcaactcatc gatttcttca tctgaaatag atgggcctaa
     2581 gccattgatc tcagtttcag tgtctgattt tagtttgata ttgattcttg tatcttcttt
     2641 tacggttgtg aacaaagact ggatttgttc tttttcaagc cacttcgaaa taaattcgcc
     2701 agtgaaccct cctaaaaacc ctagtgctgt agattgagaa tcgatccgtt tcaaaatacg
     2761 ggaaacattg atcccttttc ctccaggtaa cttgaagtct tccttcattc ggttcaaatc
     2821 gcctagtttt aacccatcta cgcgtacgat ataatcaatt gatggattaa gtgttactgt
     2881 gtaaatcatt gtattgcctc cttaattgtt gtcttttcac ggaattcatt aacgaattct
     2941 aatggacaac gctcggttat gatcgtggct gcttccaaag gtgcaacaga ggtgaaggac
     3001 aactggttga atttggtatg atcaatcaat acaaatgtct cttcggaatt tttcatggct
     3061 aaacgtttaa cagctgcctc ttctggatcc ggagtcgtga atccttgttc aagatgggca
     3121 ccgttcattc ccataaatgc tttattaaaa tgaaaatttt ttagttgttc tatcgcatta
     3181 gatcctaaaa cagcattagt tgccagcttt atcgttccac ctaaaataat ggtagcaagc
     3241 tccaaatcaa ctaaatttgc cgcatgttga acggaatttg tcactactgt gatctgtttt
     3301 ccttgcagaa aaggaatcat ttctaacgta gtagagccag cgtctaaata aatgacatct
     3361 tcgtcattaa tggtttcagc tgccgatttg gcgatttgct gtttttcgtg aacgtttttg
     3421 agtgattttt cactcatatt ctgctcaaaa ttcaataatt gctctttctt agctccgcca
     3481 tgaactcttt ctagaaaccc actattctcc agctcttgta aatcacgtct tatcgttgat
     3541 tcagaagcat tcaaaaggtt tacaagatct tgtgatttca cgattttctg ctgttcaagc
     3601 aagtctagga ttttttgtct gcgttcctct gtaagcattt tcacgatcac ctcaatacaa
     3661 ataatagcat attttttttc tactagcaat ataaatcagt caaaaacgtt cataaaaatt
     3721 ataaatcagt caaaaaaaaa taaaaaacca gtcagaaaga ctggtttttt atttttttgt
     3781 ctatcatcaa gccgttttat cgttttttat tttcgcttct tgtttttttc atataaacag
     3841 aagcaggttt tctttcctta tgaagaagat aataataaag tcccatcccg ccaaacatga
     3901 ccaaaaaacc gatgactgat cgaataggaa aattaggaac aaaaaagaga cagacggata
     3961 ataatgatgc cggaaccatt attcgataat agagttgttt atccattttc ttcacctcat
     4021 attttttgta aagtaatcaa ttgttctaat tctcggtaat ctaaaaattg cacacgcaga
     4081 tgctgctcac ctttcttgac atcgaattgg atacttcgcc gctttttctt tattcgtttg
     4141 aaggtaacat cagagagtct atgtttgatt tcctttaacg gtaattctga cagggattct
     4201 gaaggaacat agattcttaa ggataaaaga tactcttgat atttttcatc tgttacctct
     4261 acttctagat attccctaat ataataacaa taagcttcga ttccattatc caaagcaaca
     4321 gtcggatcga taactttata gcacatcatg cgtgatcgat tctcgtcaac ccaaggaaaa
     4381 tacgcctgag catttagtgt tccgcgaaca cgcctttctt tataatctcc ttgttggata
     4441 tcataacttt ttgttacaaa aaatgaagca ttctttttag gaacaggttg catttgtgca
     4501 ttctgatact ggacagattg gatcgtatgg tcagtgtaaa tgtattcaac gaatggcggt
     4561 ttcaataaac cgacaccatt gtcccaagca agatgatcaa acccaagctg gtccctaaaa
     4621 taggattgaa acatttgatt gtttggcttt tgccagttaa aggggtctcg ataaagctgg
     4681 atacatatga cttcgttttc taattccttc actttttcta cttgttggat gtcgagaaaa
     4741 atcggtttag ggatgatttg atgataatag cgactataat acatttccca acacgaagtg
     4801 aaacataaac cttggtaaaa aagatcgtat cctggaaatt ggttgcaatc gactactact
     4861 tccccgtttt gttgtttcat tttaggtaaa tgttcgattt catccttttt ctcaaacaat
     4921 agccgttgag agatctgttt tgtattgtga tagataaact cgctataagc tcgcatatag
     4981 gcgaacaccc catgagcaat catttttttc tgaatgaagt ctcgaatcaa aaattgcttt
     5041 tctttgaaaa cagagataga caatacattt tttatcaata attgattatc ttccaagcga
     5101 aaactacatt cgttctcgtc atttcctaca actaaccaac tttcgtctct taccgatgct
     5161 agcattattt tttcttgaag ctttcgaaga tcatacggat ccgcttgttg tgtgaaagag
     5221 cggataaatg ttggctggat caacagacgt gtcaaaatag cccaatcgct tatttcctgt
     5281 ttttttcttc ttcatttaag tctgtgagct ggatagtcat caataattta tcttcgccaa
     5341 tcattttctc acctctttca aatcttttta taatttaatt ataaccaatg ataaagaaaa
     5401 actaaaactt tatgtcttag aaaccaattt ggacgattac atgatagact aagtaaaaag
     5461 aaacaaagag gtgtttacaa tggggttgaa atttgaacgt acagataatt tagataaact
     5521 tttcgaatca tttgctgtcg atccggaaaa aacagttgca ccaaaagaag agatcgatcg
     5581 atttctaaaa ccacagccac aaaatgaaaa gaaaaaagac aagaaacaaa agtaagttaa
     5641 gctcaaaaaa agacttacca gccaaacttt tatgaaagag tttgactggt aagtcttttt
     5701 tattcttttt atgattcaat tgcttgttcg attgctgccc acgcttcttc ttttccctgt
     5761 tttgtaacgg aagaaaagag aataaacgtg tcattgcgat caaaatccaa cgcttttttg
     5821 atttcgctct catgcttgtt ccatttacca cgagggatct tatctgcctt agtggcaaca
     5881 acaattacag ggatatcata atacttcaaa aattgataca tctggatatc ttcttgagaa
     5941 ggcttatgac gcaaatctac taaagaaacg accgcacgta gctggtctct agtcgttaaa
     6001 tatgtctcga tcatctttcc ccattttgct cgctcagttt tggatacttt tgcataccca
     6061 tatcccggaa catcgacaaa atgtaatgcg ttctcgatca aataaaaatt caatgtctgg
     6121 gtctttcctg gcttaccgga agtacgggct aaattctttc gatcaatcaa tgtgttgata
     6181 aaagaagatt tccctacatt tgatcgtcct gccagagcta tttctggcaa ttgtgtatct
     6241 ggatattgtt ttggtgccac tgcacttatg ataatttcag cttgatggac tttcatggtc
     6301 tctctcctta acaaggtgat gaataaagca agcggctgtc gcaaaagcca aaatgacttt
     6361 tacggcagcc tcttttcatc aacccgcttt tttctctgaa tctttataga ttactgttgg
     6421 ctcaccagac aattcagctg cttcttttgt gataatcact ttttcgatgt tttcatttga
     6481 agggatatca aacatcacat ccatcatgat ttcttcaatg atggaacgaa gtcctcgagc
     6541 accggtattg cgttcgattg cttttttcgc aatggctctc aacgcttcag gttcgaattc
     6601 aagttcggta ttatctagag aaagaagttt ttgatattgt ttgactagcg catttttcgg
     6661 ctcagtcaag atccgtacca gatcatctgt tgtcaatttc tctaaagctg ccataacagg
     6721 taatcgtccg ataaactcag ggatcaatcc gaattttagt aaatcttcag gaatgatatg
     6781 ctgcatgaca ctttcgtctt cagaaagttt ttggttttgt gtaccaaatc caatcgtctt
     6841 ttcgcccata cgattcttca caatcgtttc gatgccatca aatgcaccac ctacgatgaa
     6901 caatatgttc gtcgtatcaa tctgaataaa ttcttgatgt ggatgtttac gtccgccctg
     6961 aggtggcaca cttgcaacag ttccttctag aattttcagc aaggcttgtt ggacaccttc
     7021 gccggaaacg tcacgagtaa tagatacatt ttcgctttta cgcgcaattt tatcgatctc
     7081 atcaatatag atgatccctt tttctgcaag ttccacattg taatcagcag attgaagcaa
     7141 tttaagcaaa atgttttcta catcttcccc cacataacca gcttctgtca aacttgtcgc
     7201 atctgcgatt gcaaaaggta cattcaatgt ttttgctaat gtttgtgcta aaaatgtttt
     7261 accagaccct gtaggtccga tcaagcagat attgcttttt tgcagttcaa catcatctgt
     7321 gttttcttgt gcttgctgat tgacacgttt atagtgattg tatacggcaa cagctaacga
     7381 gcgttttgcg cgatcttgtc caatcacgta gttattcaat acttcaagaa tttctagtgg
     7441 tttagggact tcagtgaatt cgcgtacagc ttcttcataa aattcttcat caatgatttc
     7501 tttacatagg tcgatacatt cattacagat gtagacccct ggaccagcta cgatcttttt
     7561 cacttcttct tgggttttcc cgcaaaaaga acagcgtacg gtctcattgc tacttggatt
     7621 gtcatacatg gtcttcaccc cttaacttgc aaaaagtatg caagaaacta tcaaagactt
     7681 tacagtcttt tactatcata acatagttta gtaaaaagaa aaagtgttta acaaaggatc
     7741 aactgaccta tacctttgca ttttttactc ttaacaaaaa ataaggaact gtgacaaaat
     7801 cgtgtcacag tcccttattt cgaataaaca attttctaga agtagcttct tcagaaatga
     7861 tttattactt gatttctatt ctttcgcttc tgtcgctgta cctgtgatca attcgattgc
     7921 ttttttcatt gtcacatcat gttccagcat atcttctgtc aacacgcgtt tgatttgttc
     7981 gattggcatg ttgtatgttt ctgataattc ttggatttct gcattgattt cttcttctgt
     8041 tgcttgaaga ttttcagcag ctgcaattgc ttcgatcaca agatttgttc ttgtgcgcat
     8101 ttctgcttca ccttcgaatt gtttatgcaa gtcttcttct gtagaaccag ttaattgata
     8161 gtacatttca ggagagatac cttgacgttg catgttgttc aagaattcgt ccattgaacg
     8221 atgtacttca tcatgaacca tcacgtgtgg caattcaacg atttctgcgt tttcaacagc
     8281 catacggatc gctgcttcat cttttgcatc gtccgctgct ttttcttttg tttctactaa
     8341 ttctttgcga tatttttctt tcaattcgtc taatgttgat acttcgtcat caacatcttt
     8401 tgcgaattcg tcatctaatt caggcacttc ttttgctttt acttcatgga ttgtcacttt
     8461 gaagactgct tcttttccag caagatcttc tgcttgataa tcttctggga aagttacatt
     8521 tacatctaga ctttcacctg atttatgacc aactaattga tcttcaaaac ctggaatgaa
     8581 tgagcctgaa cctaattcaa gtgagtaatt ttcgcctttt ccgccatcaa acgcttcgcc
     8641 atctacaaag ccttcgaagt cgattactac tgtatcgcca ttttcagctg cagcatcttc
     8701 tttgataact aattcagctt gtgcttcttg ttcacgtttg atacgtgcat ccacgtcagc
     8761 atctgttact tcacggtctt gtttttcaac aactagatct ttatattgtc ctaattttac
     8821 ttctggtttc actgttactt cagcagtgat tacccagtct tgaccttttt ccatgctttc
     8881 aacatcgatt tttggttgag aaacaggatc gatgccagct tctttgacag cagcttcata
     8941 agcttctggt aatacagcat tcaatgcatc ttcatataaa gcttcttcac cgtacatacg
     9001 gttgaatact tgacgagaaa ctttcccttt acggaatcca ggtacgttca agcttttttt
     9061 cactttatta aacgcagttg ttaatccttt ttggatttcg tcttgcgcaa tcgtaaattt
     9121 taaaacacca tcattcgtgc cttttttttc aaatgttgca gtcatttaat tccctccgag
     9181 tttagaaatt cattttatac atcaaaaacg tttcatgcat acctttaaat agtacactaa
     9241 ccctttcaaa ttgtaaacat attgaatgga tagaatcctt gtttttcata acatttttat
     9301 ctttttcact gataaaatct aatggaagaa gatcatttgc tgcaaaattt tttctctttt
     9361 ttttaaaaaa tcttgttttt catcttctgt accttcatac atcgtttttc ctgtacacgc
     9421 ctctacagtc agttgaaacc agcgcttcgg gtcttcgatg tatgtatcat agacaggata
     9481 aacaattgca gattgcaacg tgaattcttc ttttaacacg gaactaagca gcgcatcttc
     9541 attcccgtaa cgatctgaaa atacacataa ttgctggtaa cttgcctgct gctgcggctt
     9601 tggcagatct tttggtatga ctttgactaa cttttcttca atcgtcaaat agaaaacagg
     9661 atggcattcg ttcaattgcc taagtgtctc cagtaatttt gcccttgcta aaatatggac
     9721 ctctggcgat acaataagtt tactcgctgt ttggataaac tcatgataag gaagctgttc
     9781 gatctcatca atagacgcaa gctgacgtgc aggttccaac gaaggaagtt cctccgattt
     9841 attgtggagt gccttgatct tctgcctctc catttgtccg gataattctt ctgcaaattg
     9901 gattttttca atgacccgct gctcgaaagc ttcataaaaa tctgtacttg ctatcaattt
     9961 tctagcatat aaaaaatctt gtgtactgat taacaagtca aaataaaatt cagcaaactc
    10021 ctcattttga agatactgtt tttcatgcaa gagtgcttgc ttcaatgctt cttttttctc
    10081 gtccaactcg taaagacaaa aaacaagcag gctattagcc tcaaagcttt ctttcaactc
    10141 gtaagctcgt tgaaataatt cttttgcctg ctgatattgc tggtttgcta gtgcttgttg
    10201 tgccactcgc attaaattct catatttttc tggaaaatca atcggcttca cgcagtgtcc
    10261 tcctcttgct catattcagc tcatgttcaa tagtagcaaa acaaaaaaag catgacaatt
    10321 aaaaataact gaagcaccct ctaaaagcta gattttagat ctgactttta gaaggtgctt
    10381 tcaactgtta ctgcttttta ttgctcatca gctgttgact tcctggttga acgctttgat
    10441 cagattgatc gtaacaacac ttgtattagt gaaaaaatcg cccagatgtt gctttttctg
    10501 attaaagact gctggaagat accctaacat cagcaaagga aattttagaa tgaaccggca
    10561 agcaccttct ctaactaaaa cggttgacca gctaagttct tcttcatcaa agctgatgac
    10621 tcgaatacca aagatcattt tcccaatcgt ttggccatga ttgaatttcg tcaataaaat
    10681 aaaatacgct agataaataa gtaaggctgt gaatccgtag agactcagat aagaatctga
    10741 aaaattaagc caacctagat ttttggcaat accaagtgtt aaacgcgtaa tagctccgat
    10801 acacaaaaga tcgaccaaga aggcaaacat tcggataaaa aaacctgcat agaaatactt
    10861 aggaaaatca tttgcttttg gcggttcatt tcttgtgtcc gtatactttt tccatttacg
    10921 ttgtttcttt ttgatttctt cgggcgtcaa aggctctctt tcaaaagagc tcagtacctt
    10981 ttgggaaaag ttggagtcag gtcctacagc aggctgctgt tgcttatctt tttcttgttc
    11041 cttattttca tttgaaagct ctgacatgct tactcacctc cgtaataata cattgctttt
    11101 ggtgattccg ctgtccctag attctcaaga acggataaga tctgactagt ctcagaaggc
    11161 ttcaatccct ggaattctgc tagtttactt cctaaccatg tactagcaaa cccagatggc
    11221 gtggaagaat attctactag ttccgcatct tcaagctgtt gttcctttcg tatagctttt
    11281 aaagcatctt caggaaatcc caattcatcg actaaaccta cttcttttgc ttggacacca
    11341 tcataaatcc ttccatcagc aatttttttg acttcttctt cagatttatt ccgtccctcg
    11401 ctgacgattg ctacaaaacg actatatgcg ctgtcgatat atgcttgaag caccgcttct
    11461 tcttcttttg tctctggtcg cgttcctgaa cccatatctt tcaacgctcc gcttttaact
    11521 gttgtatctt ccacacccag tttttctaga agtcctgaat aattcaatcc tgacatgatc
    11581 acaccaattg agccggtcac tgtttcttct gttgcaaaga ttttatctgc ttgagcagaa
    11641 atatagtagc caccgctagc tgccatgttt ttcatgctga catacattgg gatatcatgt
    11701 tctttacgaa tcgtatccaa tttcttagcg atttccgcac tttcgtagac accgccacca
    11761 ggagaattta cttctaaaaa gatacctttg actgttggat cttcttcaat cgccttcagt
    11821 tgttccataa aatttttatg attgtaacct tcagaagcaa ataaacctga agaccctccc
    11881 gcagcaatcg ttccatctac tgataatttt acgatttttt gcgtactgtc tccctcttcc
    11941 aacacatttg gagctagttc ttcatcccca tataaccaac tgttcaatcc ctcaatttga
    12001 tcgttttcct gaggcttagt caaactagct gaaactaacg aaaccacaaa taatcctgca
    12061 gcaatcagaa ctgctgccca acgtctttta ttcattcttt ctcctttcta atacaagttt
    12121 tttatgacga tcacagtttc tgactgctca gaagtgtcga accgctctgc tttttcttcc
    12181 cgcgcatacc cggaaaacga ctcttttgtt aattcatctt ctaccgtaaa ctcacgagct
    12241 tgagcaactt ccaaggcatc aggtgcgatt tcttggatgc tatcccagcc agtatctaaa
    12301 aatattttcg tatcatctga tacaccagct gatgcttcga atttggacaa ttgttttttt
    12361 aacattccca gtgtcatgat ctcaattttt gaatccattt ttactcctcc tctatccgat
    12421 tactttctat tgtaccgaac tcctgaaaaa gatgatagat aaaaacccat tattttttga
    12481 cataaaaagg acatttgaca aagaatttga tatataccaa tttatttttt attctttttt
    12541 tgttgacaag aacattcgca aaaggttatg attttaaatg tttccagata aaaaacaaaa
    12601 aggaggatag ttataccttt taggatagcg ccaggtctgt gtaaacagat gacgaggaga
    12661 gggtttatcg aaagattcgg cggatggccc tagggactgc actctacaga aaatccacaa
    12721 aaaatgtctg caaagataaa acaaaaggat tttcctgcag ctggaagcat tcatacgaat
    12781 tcattataga aagtaggatg ttttttatgt gcggaattgt tggaatgatt ggattaaaaa
    12841 atgtaacacc aggattgatt gacggtctag aaaaattaga ataccgtggt tatgattcag
    12901 ctggtatctt tgtctcagat ggcacaactg attatctggt caaagcacaa ggcagaattc
    12961 aaaatttgaa aaacaaaata aatattgata ctacaggaaa tataggtatt ggtcatacac
    13021 gatgggcaac tcacgggcaa ccttctgaag aaaacgccca tccacatact tcccaaagtg
    13081 gacgcttcgt gctcgttcac aacggcgtaa tcgaaaactt tgaagaactt aaaattgctt
    13141 atttagcaaa tgaccacttt attggtgaga ccgatactga gattatcgct catctaattg
    13201 aaactttcgc gaaggatact acaacgcaag aggcgttttt aaaagctttg cgggtcatca
    13261 aaggttctta cgctttcgct ttgattgatc ggacagcacc tgatgttatt tacgtcgcta
    13321 aaaataaaag tccactatta atcggactcg gcgatggatt caatgtgatt gccagcgatg
    13381 caatggctat gcttgctcat acgaaggaat tcgttgagat tgaagatgaa gagatggtta
    13441 ccgtcacatc agaaaaagtt attattcaaa attttgcagg tgacatcgta gaacgttctt
    13501 cattcgaagc acaagtagat gcttcagata tcgagaaagg tacctatcct ttttatatgc
    13561 tgaaagaaat cgacgaacag cctattatca tgcgtcgtat cgctcaaaaa tacgtaacag
    13621 aagacaatcg tgtctctctt gatcaaaagc tggtcgatac attgtctgat agcgatcgaa
    13681 tctatatcgt tgcttgcgga actagttacc atgcggggtt agcaggtaaa cagactttgg
    13741 aaaaattgac acagattcct gttgaggttc atttagccag tgaatttggc tataacacac
    13801 ctcttctttc aaaaaaacca ttctttattt tcttgagtca aagtggcgaa acagcagaca
    13861 gccgccaagt gctggtaaaa atcaaccgtc tcggctaccc ttcacttacg attacaaatg
    13921 ttgctggttc tactctttcg cgggaagcat cctttacact gctattgcat gctggacccg
    13981 aaatcgcagt agcatcaacg aaagcttata ctgcacaaat agctgtacta actttattag
    14041 ccaaagctat cggtgataaa aaagaatttg aaacttctct tgtattcgat atcactcatg
    14101 aattgagttt agttgctaat gcgatggaat cggtcatcgc ccaaaaagat tatttagaag
    14161 agttggcaga agtatatctc agcgatacaa gaaatgcctt ttacattgga cgtggtgctg
    14221 actacaacgt ttctcttgaa gctgctttga aattgaaaga aatcagttat atccaggcag
    14281 aaggttttgc cgctggcgaa ttgaaacatg gaacgattgc tttgatcgaa gaaggaacac
    14341 cagtgatcgg aatcatcagt gaagaagtga ctggtgcgca tactcgagga aacctgaaag
    14401 aagtcgaaag ccgtggcgct aaaacactag ttattgtctc agagggatta gaaaaggaaa
    14461 gtgaccagct ggtgctacca actgtccacc cttatctaac tactctaacc acagtcgtac
    14521 cgactcaatt acttgcatat tacgcaacgc tattacgggg attcgacgtt gacaaaccac
    14581 gtaatttagc gaaatcagtg acagtagaat aataaaagaa caaggaccag tgtcagacat
    14641 ctgacactgg tccttgttct tttatttttt atgaaaatat ctcttaatcc agtccgattt
    14701 cttctttgac tactgccgcg attttatcga catagtaatt aactttctca tcagtaggcg
    14761 cttctgccat cactcgaagt aatggttctg taccggaagg acgaactagg attcttcctt
    14821 ctgatcccat ttcagcttct gcctcttcaa tcactttttt gattgccgga acatccatcg
    14881 caccgttttt atcacttacg cggatattca ctaatttttg cggatagatt gttacctctg
    14941 ctgcaagctc tgataatttc ttacctgtct gtttcatgat attcagtaac tgaattcctg
    15001 aaagcatccc atctccagtt gtgttaaaat ctaagaaaat catgtgccca gactgctcgc
    15061 cgccaaagtt ataatcgttt ttgcgcattt cttctacaac gtaacgatca ccaacttgtg
    15121 taacgacatc tttcaaaccg attccttcca ccgctttgtg gaatcctaag ttactcatga
    15181 cagtcgtaac gatcgtatct tttttcaatc gatttttttc tgccaagtat ttagcacaga
    15241 taaacatgat tttatcaccg tcgacaatgt ttccaagttc atccaccgca ataatacgat
    15301 cgccatcgcc atcgaaagct agtccagcat ctgctccttt ttcaacgacc atctcagcta
    15361 aacgttctgg atgggtagag cctacgccgt cattgatatt taacccgtct ggatttgtgc
    15421 ccattgtata aaaatctgtt tcaagatcgg caaacaaacg attaacactt gttgaagttg
    15481 ctccgtttgc tgcatcgaca caaatggtca aaccggaaag gtcaccgttg atcgtttgta
    15541 caaggaattg agaatatttc aataagcctt ctggaaattc ttctacagtt cccaatcctt
    15601 cagctgatgg acgaggcaat tcatccactt cagcatctaa taatgcttca atttctagct
    15661 cttggtcatc tactagttta aaaccatctg caccgaaaaa tttgattcca ttatcttgtg
    15721 ctggattatg agaagcagaa atcatgaccc ctgcgcttgc tttttgcaaa cgtgtcagat
    15781 aagcaacacc tggtgtagag atcacaccta gctggaacac ttcgataccc accgataaaa
    15841 gtcctgaaat cagcgcttct tctaacattt gacctgagat tcttgtatcc cgccctacta
    15901 acacgcgtgg gcgttttcct tcttcttcat gctggcttag gacataacct ccgcaccgtc
    15961 ccagcttaaa tgcaagttct ggcgtaagtt ccttgttggc ttcaccgcgt acgccgtctg
    16021 ttccaaaata tttacccata gttgtaaaat tactccttta acttaatttt ctgaggaatt
    16081 tgttctgctc atttgtgttg attcgcttgt ttctgatgtt tcttctgtac ttgtggtcga
    16141 gctgcttgtc ccagattccg aactggaaga aatggttgtt gtcgatggtt ccgttcctga
    16201 atcagcagat gaaatacttc tactcgtact tgtattttct tgtgtccgac tgctcgtcga
    16261 agcagagcta ctgcttgacg tttgcaacac gggtgtaatc tccacactaa ctgattcggg
    16321 cttgatactt aacccctctt ctactggtat ttcaatttta cgagttgttc gatgattgat
    16381 acctgagaca ttgatcggta cagcaatact gtcaccgatc gatgcaagca aatcgctaga
    16441 gccgcttact tctgcttcca gttttgaaaa actgaaatca tagctttcta ccccggcagc
    16501 tggtgttcct tgctgtatcc cataaagtcg taccgacttg cttggcttga tcgaatcagc
    16561 tctcactgtt acttgcactg gatcagagac gatacttaat acttcccctg ctgcattcag
    16621 cgcctgtatt gggacctcac tattaatccc atcatctgtg accatcgaag cgtccacatc
    16681 agctactact cgtttgattt ctgccaatgt tttgtcacct gtagtgatag agactttttt
    16741 aggactaacg gaagtatcat caagttcata tccagatggt gtgaacgttg aaggaatcaa
    16801 cgtttccaca tcgaaatctt ttattacctt tttctcaacc gttactgtaa tagtcgatgg
    16861 ttcgatcttt gctgtgacag cactgctcaa attttgaatt tttagcggaa cttcatgtgt
    16921 tccttcgccc agcttagtga tatctgctgt gacgcggaac atccgtgtat cttcgtttgc
    16981 ctctgcattc aactgaactc ggtttgcact ggaaagtttg acagtcactg cattttcata
    17041 tccatggata tagtagcgct ttgaatcata agaaatcttg atcggcaccc ccgtaactgt
    17101 ttgattatac gcttcatttc cagaaagcgt atttggaacg gactggccat ttgcattgaa
    17161 gtacagcgca atactgaaca gcaatgcaat aaaactgtaa agaatgcttg tccgtttctc
    17221 ttttgagatc atttgcgatc ccctccctta gtcataccgt ctaggaaaga ttgaatcacg
    17281 tttttcttgt tttttgtttt ctcttcagca attagttctc tacgcaagat tgccaaatat
    17341 tcttgctggg ataagccagc catcagttca ttatctaacg tgatgctgac gtccccggtt
    17401 tcttcagaga caacgatggt aatcgcgtca ctgacttcgc ttataccgac agctgcccgg
    17461 tgtcgagtcc cgaattcttt agggatcaac atactttctg atagaggaag ataggcgctt
    17521 gctactgcga tctttccttc ttttacaata actgcaccgt catgcagcgg tgtattagga
    17581 ataaagatat tgatcagcag ctccccagtg atatctgcat ctaaagcaat ccctgtctca
    17641 atatactcat ctaatccagt atgacgttcg atagtgatca acgcaccgat ttttcgcttg
    17701 gacatatatt ggatggcttt gtcaaaagac aagatcatct tctcatcttc ctgctgttcg
    17761 ctttttgatt gtttgaaaag cgaactgcgt ccaagatgtt ccagtcctct ccggatttcc
    17821 ggctgaaaaa tcacgatagc agctatcacc ccgtacgtaa tgacttggtt catcagccaa
    17881 gacaaggtgt gcagaccaat gatttccgct acgattcgga taacaataaa aatagctaca
    17941 cctttcagaa gctgaatcgc ttttgtgcct cgcagcagtt ggatcagttt gtatacaaga
    18001 taccagacaa ccagaatatc aagtatgttc accaaaaaat tcagagagaa caagtcaccg
    18061 gaaaacattt gctgccaata gtccaaatta aaaagttccc cgatttgaaa tgacataaaa
    18121 agcctctctt ctcttttgag atttcctctc tagtatatca tatgttttaa ttttagaggc
    18181 tgatatgctc gtatttttct tgagaaaaac gcataatttt caagaaaatt gcaagtatgc
    18241 gttttatttt tctctgcaat aaatattttt aaacttcaga gaattattca ttatattttt
    18301 tctgttattt ttcctttttt tcttttgtag cttgttccat ttttttacgt cgttctgctc
    18361 tggcagctct tctagcagct cttctttctt ctgcttcaag gtccgtcgtt ttttcttttc
    18421 tagtcttaac gggtacatcg atagattcct ctggttcaag tcgtttcttg attttttcta
    18481 attctcccgt cagtgcagca tagttgtaaa aacgtgtttt agcgtcgtgg acagtctttt
    18541 caaataaagc ttttgcaaga tttggatttg cgttatctag cgaggaaaag cgaacttgtt
    18601 tcctcatgaa atcaggcata gcagagaaat caggtttttt gtagtccaaa gtcattgggt
    18661 tcttgttttt ttcgaccatt gtagggttat atcgatataa tgaccaataa ccagaagaaa
    18721 cagcttcttt tgcttcttta agcgattctt tcattccgcc aagcaagcca tgtgtgatac
    18781 aaggcgtata ggcaataatc agagaaggac caggatactt ttctgcttct tcaaatgcct
    18841 tgatcgtttg catttgattg gcacctgaag caatttgcgc cacataaaca tttccatatg
    18901 tcatcgccat cattcctaga tctttctttg aagcatactt tccactagcc gaaaatttag
    18961 cgattgccga agctggtgtt gcttttgagg tttgaccgcc agtattggaa tagacttcgt
    19021 tatccaaaac aaggatattc acgtctgctc cgctggcaag tacatgatcg atcccgccaa
    19081 aaccaatgtc gtaagcccat ccgtctccac caatcatcca ttggctgttt tttacgaata
    19141 gatcctttct cttgtatata tcctccagtt cagggaaaat tgatcgttcg ctttcgatcg
    19201 cagcttgcag ttttgctgct ctttgctgcg ttccttcact ctcaaacatg tgtactaacc
    19261 agtcttccat caattctcgt agttcttctg atacttttgg caatacttct ttgattgaac
    19321 ttgccaaagc ctcgcgacga gtctgtgccg ctagaagcat accataacca aattcagcat
    19381 tatcttccaa taaagaattt gaccatgcag gcccttgacc acattcattg gtcgtataag
    19441 gagaagcagg agaagcaccg ccccaaatag aagaacagcc tgtcgcattc gcgatcatca
    19501 ttcgatcacc gaacatttgt gtcaataatt ttacataagg tgtttcgcca cagcctgagc
    19561 aagcgcccga gaattcaaga agcggttgat tgaattgtgt agacaagaca gtattctttc
    19621 ctttgactgg atttgctttt tgtcttaatg tcattgcaaa agcccaattc acagcttgtt
    19681 ctttctgttc ttcataaggc ttcatcacta aagctttttc ttttgcggga caagcctcta
    19741 cacacagacc acaacctgta cagtcttcca ctgaaacttg gatacggtat tttacgccgt
    19801 ctgcgcctcg catttctctc acgataaatc cttctggcgc ttcttccatt tcctcttcat
    19861 ctgctaaaaa tggacggatg gcagcatgag gacaaacaaa ggcacattca ttacacatcg
    19921 tacagcgatc actaatccac tcaggtactt ctaaagcgac gccccgcttt tccatagaag
    19981 ccgttcctaa aggcatttcg ccagttgtca ttccatttgc tacaagtgta ccaacagaga
    20041 gttgatttcc ttcctgtgca ttcactggtt caagtatttc ttgtatataa ccagtgttcc
    20101 ttttattgat tttttcaggc atctccaacg ttctccaatg gtctgggatc gcgacttgat
    20161 gaagcagttc ggtcgtccga tcgattgctt gtatgttttt ttctacgata gccattgatt
    20221 tatgtccata gcttttcagg gcttcttctt ttagcagcgg aagcacctca tcaaaaggaa
    20281 tgatgtcagc taatttaaaa aaagccgttt ccattgccgt attgatccgg cgtcccaaac
    20341 cgacttcttg ggccaatctc atggcattga tcgtatagaa tctaatgtta ttttcagcca
    20401 agtaacgttt taattttaag ggcagatgcg tttctaattg ttcgtcgctc caaagagtat
    20461 tcaataaaaa aatgccacct ggcttcaatc ctttgaccaa gtcatagcta tgcaggtaag
    20521 ctggtgtgtg acaggcaaca aggtccgcat gctcgacaag atacgctgaa cgtatcggcg
    20581 tatctccaaa acgaagatga gagacagtca gaccgccaga ttttttcgaa tcataataaa
    20641 aatagccttg cgcatattta tcggtgtgat ctccaataat cttgatagct gatttatttg
    20701 ctccaactgt tccgtcagat ccaaatcccc aaaacttggc ctggaatgtt tgcggttctg
    20761 tcaaatccag actttccttt ggttccaaag attgataagt cacatcatcc acgatcccta
    20821 tggtaaaccg ttttttgcgt atgcttgggt cttttttcaa ttcatcaaaa actgctgaga
    20881 tttggtcggg agtcacatct ttagacccca atccgtagcg accaccaata acagccgggc
    20941 gaagttcaga atcatataac gcactttgaa cgtctaataa gagaggttct ccacctgccc
    21001 ctggttcttt acttcgatca agaacagcaa ttgtttttac tgtatgaggc aacttttgca
    21061 cgaatgtttc cactggaaaa ggacgataaa gatggatatt caaaaaacct gttttccgtc
    21121 cgtttgcatt caagtaatca atcgtttgtt caatcgtctg tgcaactgat cccatagaaa
    21181 caatgacttc ttccgcatcg tctgctccgt aataagtcac caagtcataa gaagttcctc
    21241 gtaatcggtt gatctctttc atatattttt gaacgatttc aggtaattta tcataatagg
    21301 ggttgatcgt ttcccgttgt tgaaaatgga tgtctggatt ctggttcgta ccactaacag
    21361 tcggatgatt gggattcatc cctcgtttac gaaatgaagc taatttctcc tgattgatca
    21421 gaggagctaa ttcttcatat tccaaaactt cgattttttg aatctcgtga ctcgtacgga
    21481 atccatcaaa gaaattcaaa aaagggactt ctccttcaat agaagcaaga tgggcaacag
    21541 gtgctaaatc catcacttct tgtacactgc tttcagctaa catagcaaat cctgtttgtc
    21601 ttgcagccat cacgtctccg tgatctccga aaatattcaa cgcatttgtc gtcactgccc
    21661 gactagcaac gtggaaaaca cttggcagta gttccccagc aatcttgtac atattcggta
    21721 tcatcaaaag cagcccttgt gaagcggtat atgtagttgt cagtgcacca gttttcaatg
    21781 atccatggac cacaccagct gctcctgctt cggattgcat ttctactact tttactggtt
    21841 gaccgaatac gttttctttt cctgctgctg accattgatc tactaattca gccatggtcg
    21901 aactgggtgt tattggatat attgcagcaa tctcggtaaa tgcataagaa atatatgcgg
    21961 ctgccgtatt gccatccatt gttttcattt ttcgcatgat tcatcatcct tgttcatatt
    22021 ctctcttatt atctgttcca ttataacgta aaacatgcca tttttttctt ttatttataa
    22081 aaatatttta catgtatcta tttgaattgg tcatctaatt tttagacaaa cagtctaaat
    22141 cgttgccttt ttttgtcaga catgtataaa ctataagcga aagaggtgtt ttaacatgga
    22201 tatatcagtg attgatgcaa cgaaaacaaa tacacaaaaa ggaatccttt atggttcatc
    22261 gaatgctcct aaaaaaatgg tcgaattcat caacttagct tgtccgtatt gccgacaatg
    22321 gtttgaagaa tcttatgagc tattagaaga agcagtccaa agcgggcaat tgcagcgagt
    22381 gatcaaattg ttcgataaag aaaaagaaag cttacagcga gggaacgtga tgcaccggta
    22441 tctcacaatc agcgatggcc aaaaagcgat caaagagatc aaacaaatat tcgatactca
    22501 agacgagtgg aaacatcttt ctctccaaga agtagcggat tttgcagtag ataaactaaa
    22561 gctgacagaa caaaaagacg aacagctgag ccaagctgtc atcaacgaag ctgagcaagc
    22621 acatattcga tttgttccaa ctgtcatctt gggtaaagaa atctttgatg aatctatttc
    22681 aatagaggaa ttaaaagaat tgatccaaac aaaataaaaa aataaggagc gtgggacaaa
    22741 agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag cagaagcaac
    22801 tccttcttat ttcttggagt taaacgcttc tgtcccagcc tccttattcc gaataaatgg
    22861 tgtccaaaag cttgcatctg ccagattatc tcatttctta tgacaagcta agcaaattat
    22921 ctccaccaaa taatttattc atatttttga cttgcgcata ctaactaatt cttaaatttt
    22981 catacaaaat ttctcaattt cccttaacga gtaatattta tgtacggtga ttctcactag
    23041 tatcccaatc actttttaag ttatcccgat ttcaattcta cttcaaaaat agttgctttt
    23101 atgtatgctg ttagttgatt agatggtcat ttgttgagct aggtcgttta ttttaaacaa
    23161 gttgtttctt tagcttatac ctatctcgtc taccaaacgc aagatggcaa acacaaatgc
    23221 atacgcatct gtatttgcca ctttgattcg tataaagcta acaactatta actcctctca
    23281 gctaatagtt gctctctttt ttattcagat tcatttttgc tgatcacagc ctagtatttc
    23341 aatggttttg aacactaaac caaattgttc cattacaaca ttcactggct gatgaaatgg
    23401 attagtagat ttcgattatt caaagcgatt ttctattggt ggatcgtgtt cccaactaat
    23461 aagacggatt ccaaacctat cgtacagatc ttctaaacca cttataaatt ttgcacctat
    23521 ttcaggtgtt ttattgcgtg gatattctat tgttacgcca ttaaatggat accaattagg
    23581 attaggctct ctttttttct gtacatctcc catagaatag ttagcacttt ctgggacata
    23641 atatattctt cgaccatcct ctaattcatc taataagata tttcgtgatc gtctatcact
    23701 tgctaaatgt agacgccacg tgcgatacca tgccatgcta cctgcatctg cagaaaatcc
    23761 atcaaataat gcaacaattg tatcttctat cacaatcctt tccccatcta cttcatattc
    23821 taatctaaaa tcaaactccc ctcgggtaat ttctggtctt ggtggcttag gacccaatat
    23881 tattctactt aaaaccgcat tcgttcccag atgaaacaca gctataacaa caataatgat
    23941 taatattttg tttctatttg ataaatttct cattattcgc acatcacctc tacattctca
    24001 catattagtt cgtaatactc ctttaatatc aaaatatcta ctggtcgtaa taatattcgc
    24061 tatgaattcc tactactcgt ataagaaagg ccaaatcatc agcttttact tgctgatgat
    24121 ttggcttgtt tctatttatc gtaattgttt tctaatcacc aaaattgtta acaatcggtt
    24181 cagatatttc ccagttgatt aaggtaattc cgtattgtgt caaaacttct tgcgcttctc
    24241 cgaaattaag tacagttctt atgtatccac tttctcctaa tataaagtct acctctcttt
    24301 taaaaccttc ctcgagattt ctaagggcta tatgtggttt taatgacatt gattgtacat
    24361 tttcccccat ataatattgc gcacttcctc gactaaaata gattcctaga tcgtccccaa
    24421 aaggtagatc ttctctacca ctagctaatc tacttctcca tcttcgttct cttttcatag
    24481 aacctgcgct gaactcacac ccgttaaact cagctatgac agtatcttca attatgttaa
    24541 tctgtccatc tagttcgtat tcaatcctaa atggaaattc tccataggtg atttccggtt
    24601 cgggtatttt aggtgagatt aatgcaaaga aaggaaatat taagcttggc aacatcaaaa
    24661 aaactcctcc tatgatcaca attcccgatg taatccaaaa tataatttgt acaattctca
    24721 ttgatccatt gtattccatt ttattccccc tattactatt catatccgcc tatctatatc
    24781 gatcttccaa tgcagagata attgcttcca taccttgatt ttttactgag ttacctatgc
    24841 gagatatcat tctccactaa ggtgcatcat attgtctatc taacatgatt aattctccaa
    24901 taggtgtata ctcctccaaa aacaacattt aatgcatctc cctcgacaac ataagctacc
    24961 atgtttgctg tatatgtgtt tgagtctaat tcataagcag ataaatttac tgtcgctgga
    25021 ttaaatataa tcgcattcgt atttatagct actgaatttg ctgcttcagc accacctttt
    25081 gaatgtccga tcattgtgat ttcaatagta ggatttacgt ggacagccct caccgtttac
    25141 cctgaccatt atagaaatcg cttacttttt tgaactacgc aacaaaggat acaatgctcc
    25201 taacttcact gtctccattt ttgtccacta aggaatataa aaaataggca ttaattcatt
    25261 taattcggtc acaacacgtt accttttaaa tcattatact gtatctcata cattattatc
    25321 aataaactat tataaaaatg caaaaaatgc tttatcaatc tattttgtaa ttggtcataa
    25381 tatcattgaa atcataccct gtatcgattt ttcctatgaa tctcgtctat ttgtgattga
    25441 cgaaaaagag gcaagacaga cactggaatg gtaattttac ccactatttt gctccaacta
    25501 tttgtaatcg tacccacttt tgaaataccg agatgatcat tttaattgat atgctgattc
    25561 ccaagcaaaa ccccaagaaa ttaaatgctc tatcgcatct agcttcttgg gttttgctta
    25621 tataaattcc aacatttaaa ttttttatgc tgttcggtct cagaaatgcc gtttttgctt
    25681 atatcacaat atcaagatgt caaattattc gtgttgccga tgactttgat taattttttt
    25741 caaattgttt attaagacta atgctgtctt tttgcagata ccaatgttcg aactttttaa
    25801 cattatcata tgatcctgca agttttaact tcttaccatt agatgttatc aaagttataa
    25861 ttaaccttgc ctttttccca ttaggatctt ttattttatg aattttctgc aggtaatctc
    25921 tcctaatggc agctattttt acctcatcag tgctgataaa ccattgatca tccacataca
    25981 tactgcctgt tatgttgtgt ttttccattt cttccgagaa cgaaaagttt aaggctcgtt
    26041 cttacttcca aagttgaatg agtccatgga ttggacttag tgtgagaatt gagaacaaca
    26101 ataagaatcc atatcctccc gcaaacatcc ctaaggctat actgtctctc catccataag
    26161 taggattaga agagaaaaga gtcaccgttt gtaataatat gaaaaggaat aatccagcac
    26221 aaacaccaaa gataacaact ccaaacattg aatttttata caatgaccat ttatttatag
    26281 gcttttcttt cattttactt ccccctactg ttctgttcac acggaatgaa ttcaaagttc
    26341 ataagccctc ctctttgaac atatgaatac gacttgctaa ctatacttta aaaaagcttg
    26401 ttttaagcgg ttataggcga gttgtaatct ctattcatct cattagcaaa taacttgccg
    26461 aaaagtctac tgaaattaaa gcagatagtg gaagaattat ttagccagga tagacactat
    26521 ttgtgaaaca catgtcttta cttttgaaat tctatgatga aaagccttgg aatacagtag
    26581 ctcttaattg gtaatacttt ttccattata aataacctct tttcatttta tatttatccc
    26641 ctctaaaaat aatatctaat ctaatatttt aaacttatat ttccctcgct gtgctaatag
    26701 agcattacta aataaataga aactagaaaa aagcattacg cctgccagaa taaaccaatt
    26761 atttgtat
//
LOCUS       DACCYH010000032        26641 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.032, whole genome shotgun sequence.
ACCESSION   DACCYH010000032 DACCYH010000000
VERSION     DACCYH010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.032"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            476..718
                     /locus_tag="IVY85_001305"
     CDS             476..718
                     /locus_tag="IVY85_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAP8044331.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            715..1185
                     /locus_tag="IVY85_001306"
     CDS             715..1185
                     /locus_tag="IVY85_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8044332.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            1313..2527
                     /locus_tag="IVY85_001307"
     CDS             1313..2527
                     /locus_tag="IVY85_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8044333.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(3237..3425)
                     /locus_tag="IVY85_001308"
     CDS             complement(3237..3425)
                     /locus_tag="IVY85_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044334.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            3516..3725
                     /locus_tag="IVY85_001309"
     CDS             3516..3725
                     /locus_tag="IVY85_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAP8044335.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            3837..5450
                     /locus_tag="IVY85_001310"
     CDS             3837..5450
                     /locus_tag="IVY85_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAP8044336.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            5485..6696
                     /locus_tag="IVY85_001311"
                     /pseudo
     CDS             5485..6696
                     /locus_tag="IVY85_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            6719..7645
                     /gene="glsA"
                     /locus_tag="IVY85_001312"
     CDS             6719..7645
                     /gene="glsA"
                     /locus_tag="IVY85_001312"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP8044337.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            7853..9313
                     /locus_tag="IVY85_001313"
     CDS             7853..9313
                     /locus_tag="IVY85_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044338.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            9534..11297
                     /locus_tag="IVY85_001314"
     CDS             9534..11297
                     /locus_tag="IVY85_001314"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP8044339.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            11381..11839
                     /locus_tag="IVY85_001315"
     CDS             11381..11839
                     /locus_tag="IVY85_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP8044340.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            11948..12631
                     /locus_tag="IVY85_001316"
     CDS             11948..12631
                     /locus_tag="IVY85_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8044341.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            12778..13392
                     /locus_tag="IVY85_001317"
     CDS             12778..13392
                     /locus_tag="IVY85_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP8044342.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            13408..13614
                     /locus_tag="IVY85_001318"
     CDS             13408..13614
                     /locus_tag="IVY85_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044343.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            14155..15285
                     /locus_tag="IVY85_001319"
     CDS             14155..15285
                     /locus_tag="IVY85_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8044344.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            15377..16132
                     /locus_tag="IVY85_001320"
     CDS             15377..16132
                     /locus_tag="IVY85_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP8044345.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            16190..18229
                     /locus_tag="IVY85_001321"
     CDS             16190..18229
                     /locus_tag="IVY85_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP8044346.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(18338..19489)
                     /locus_tag="IVY85_001322"
     CDS             complement(18338..19489)
                     /locus_tag="IVY85_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8044347.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(19643..20173)
                     /locus_tag="IVY85_001323"
     CDS             complement(19643..20173)
                     /locus_tag="IVY85_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044348.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(20272..20622)
                     /locus_tag="IVY85_001324"
     CDS             complement(20272..20622)
                     /locus_tag="IVY85_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP8044349.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(20779..22308)
                     /gene="ahpF"
                     /locus_tag="IVY85_001325"
     CDS             complement(20779..22308)
                     /gene="ahpF"
                     /locus_tag="IVY85_001325"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP8044350.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(22318..22881)
                     /gene="ahpC"
                     /locus_tag="IVY85_001326"
     CDS             complement(22318..22881)
                     /gene="ahpC"
                     /locus_tag="IVY85_001326"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP8044351.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(23025..23687)
                     /locus_tag="IVY85_001327"
     CDS             complement(23025..23687)
                     /locus_tag="IVY85_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8044352.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            23871..25517
                     /locus_tag="IVY85_001328"
     CDS             23871..25517
                     /locus_tag="IVY85_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8044353.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            25508..26590
                     /gene="uxuA"
                     /locus_tag="IVY85_001329"
     CDS             25508..26590
                     /gene="uxuA"
                     /locus_tag="IVY85_001329"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8044354.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
BASE COUNT         9044 a         4559 c         5332 g         7706 t
ORIGIN      
        1 tttttccgcc atgcttgcaa gggggcggtt cgcttcggcg gttcggcgag cttgcgagcc
       61 gttcaaagcc cccgttatct aacaaggggg gcacaaaaaa caagaaacac tatacactaa
      121 caagcggaaa acgctgtatt tataaggcaa aaccccattt ttacagtagt gatataaatt
      181 gtacctatat cactatgaaa attgaatagt aaatatgaga aagacagttg atttcaaaaa
      241 ataagaaatt gattgtcgtt ttcttttgca gaaatttaag tgcaggcaat tatggtagtt
      301 gtcgtaattg ctgtcagctc agtgtttatc actgatggtg tgaaagattt acggttggag
      361 atgttcaata agattattgg tgcttaatta tttttattgt agtgctgata tgagaatgtt
      421 ataattgggg tataaaacat acaaataaat ttgctgaaaa atgaggtatc aaaatatgga
      481 aaatgaatta ctggaaaact taggaagact tcacacaact gaacttggcg ttattagaat
      541 taaaaagaac ttatctttga atgtagaaaa tgtaattgaa tggtgcaaag aaaaaattag
      601 cctttctaat gcggaaatca caagaaaagg aaaaaactgg tatataacga ttgataattg
      661 tattataacg gtaaatgcgt acagttatac cgttatcact gctcacaagg taaaatgata
      721 aatttttcta taaaggaatt aaacgcattt gcagttattg ggcaggaagt agaactgact
      781 aattatcaaa aaaagaatat tcagattagt acacagtttt ggagaaagtt taacagtagt
      841 ttgaaaaaag cgtatctttc acaatcagga aattgggtaa aatatgcttt tatggaaaga
      901 agaaatggaa aactttatta tttctgttct attccccaaa gaaccattac cccagagggc
      961 tttctgtata aagaaatacc gtcttataaa tatttggttg tggagcatat tggtgctatg
     1021 gataaaatat atgaaactta tggcaagatt tatgaagaaa ttattcctag tacatcgtat
     1081 attcctataa aagatattat cctacacttt gaaaaatacg attatcgttt tcattggaat
     1141 agtgataatt cagttattga aatttggata cctattcaag attagaagca attttatatc
     1201 catacacaca aagcagttag tcttaggatt gactgctttt tctatacaga tttttaatga
     1261 caggcaatta tcaattatgg tagttgcctt ttttgattgg aggaattgaa gaatgaatga
     1321 taaaaacttt attgaagaac taagacaaaa gcgtgaggaa tacggagtaa cacaaacaag
     1381 gcttgctgtt gcctgtggta tcagccgtga atattacaac cgcattgaaa aaggaaaaca
     1441 gccattgaat gacgaattaa gagaaatcat agaaaaacag attgagcgtt tcaatccgca
     1501 agaaccacta ttcttattga ttgattactt tcgtgtccgc tttcctacta cggacgcatt
     1561 ggcgattatc cgtgatgtat tacaactgaa atctaattac atgctttatg aagattatgg
     1621 aaaatacgga tatgaaagca aatatgtact tggcgacatc aatatcatgt gttccatgca
     1681 ggagcattta ggtgttttat tggaactgaa aggcaaaggc tgtcggcaaa tggaatgtta
     1741 tctgctggca caggaacgct catggtatga cttcatgctg gattgtatga cggctggtgg
     1801 tgtgatgaaa cggcttgatt tggctatcaa tgataaagca ggaatattgg atattccaaa
     1861 gttaaaggaa aaatacaagg ctggcgaatg tatgtcctac ttccgtatgc agaaagatta
     1921 cagcggtaca gaaaaatgcg gtagcgattt accaaagaat acaggagaaa ccttatatct
     1981 cggttcaaca agtagcgaat tatatatgtg tgcttatcag aaaaattatg agcagtatgt
     2041 caagaatggc acagaaattg aagatacgga gattaagaac cgttttgaaa tacgcatgaa
     2101 gaacgaacga gcctattatg cggttgtaga tttactgacc tatcgggacg ctgaacgcac
     2161 cgctttttct atcatcaatc attatgtccg ctttgttgac agagaggacg acaagccaaa
     2221 aagtcaatgg ataacaaatg atgattgggc atggtttgta ggggaaaaca gagagccgat
     2281 acgcttaacc acaaagccag agccttacac tttacaaaaa gcgttgcatt ggctacaaag
     2341 acaagtcgca ccaaccataa aaatggtaca ggcattagac agagaaaatc gtacaacgat
     2401 actgaaagat atgattgagc aggcagaact aaaagacaag cataaacatt tattacaatt
     2461 agagaaatca accatagaag aacgtataga taccgctgtt ccacaagaga atgacggtat
     2521 tttttaatgt aattgaaaaa tttcagattt agtcagcaaa gagctgtttt cattccctat
     2581 gtgaagtgaa agaagaaaaa taaatttttt attaaatctg caacaaaatg gctacttgcg
     2641 tacagatatg gtgcgagaaa aggaaatcta aacttttttt gaaaataggt cattaaatcg
     2701 gggcttgctg tccctatatg aagtgaaaga agaaaaaagt tttgaaaaaa tgacggaaat
     2761 tcgtgattag tggacagtct tctgtgaaaa gaggaaaaac atcatcattt tgtattcaaa
     2821 caggcattag cagttgagtg ttgatgtgaa aagatgaaaa tactttgttc tcaacttaac
     2881 aatttaaggt ttccgttccc tatatggtgc aaagaaaata tttcattcta ttgttggcag
     2941 taacggtatg gtttgggtag ttagggtgtg aaaaggaaaa tattcttttt tcatcatcaa
     3001 cttagcaaaa tgacattttc tatccctata tggtgcatga aaagaagtta aaatttttcc
     3061 atttaaactt agcaaatgga cgtttgctgt acagatagta gtgaaaaagg agaaaatcaa
     3121 ccgttgattg ccaaaatgaa ccgttccatt tgtactaata atggaaaaag ttttggattt
     3181 tggttacgtt tctcctcttt tccaacgcga tatataggaa agacgatttt tttctttcaa
     3241 tcgttctttg acaactgaat aaagctattc aatacgtttg acagacagct agccgttgaa
     3301 acaaatacgc cacgaccttt cccctgcaag agcgagcgaa aacctatttg tgttcttgta
     3361 aaagatttgt cctcttttat cgccatgacc tgtactgtca gataatgata ctcccgtaca
     3421 gccacagctt gagcattaag agcgtcgcac gcaatgggta cggctacata agaactatgc
     3481 agaggtggaa ctcctgtggg ctatgacaaa agccgttgaa ttgcttagaa agattttaca
     3541 gaaagggggt atgtgttatt gataatgctg taaaaacaat tcaaaagaaa aatggcaagt
     3601 gtggcattgg caacagagga aagacaaaga ttgtagtaaa agagcatttt tccgaaaagg
     3661 gcaaaacaat ggaagaactt ctaactgatg tcatgctgga aaaagcaaag cagacaatcg
     3721 cataaaatcg gtgcagttgt aagaaataga ttgaatgaca aaaagtaccc gtgttatact
     3781 ttacttgcaa gcaggtattg taaacacggg ttagttataa aggaggataa ctgacaatga
     3841 aacaacagat ttacaatact gcactttatc ttaggttaag ccgagatgat gaattacagg
     3901 gcgaaagttc cagcattacc acacagagaa gtatgttgcg tttatatgca aaagaacatc
     3961 atttgaatgt cattgatgaa tatattgatg acggctggtc gggaacaaat tttgacagac
     4021 cgagttttca aagaatgatt gaggatatag aggcaggaaa aatcaactgt gttgtaacga
     4081 aagacctttc ccgtcttggc agaaattata ttatgacagg acaatataca gaattgtatt
     4141 tccctagtca taatgtccgc tacatagcga ttgatgacgg tgtagacagc gaaaaaggcg
     4201 aaagtgagat tgcaccattt aagaacatca tcaatgaatg ggtggcaaga gatacaagcc
     4261 gtaaagtcaa atcagcattt aagacaaagt ttgcggaggg ggctcattac ggtgcttatg
     4321 ctccgttagg atataagaaa caccctgaca tcaaaggaaa actgttggtt gatgatgaaa
     4381 caaaatggat tattgaaaaa atcttttccc tagcctatca aggttacgga agtgcaaaaa
     4441 tcacaaagca gttaagagca gaaaaagttc cgacagcgtc atggctaaat tttacaagat
     4501 acggtacttt tgcacatatc tttgagggaa aacccgaaag taagcgttat gagtggacga
     4561 ttgctcatgt caaggcgata ttgaaaagtg aggtttatat cggaaacagt gttcacaata
     4621 tgcagtctac ggtatcgttc aagagcaaaa agaaagtgcg taagcccgaa agtgaatggt
     4681 ttcgagtaga aaacactcac gagccgatta ttgacaagga agtgttctat cgtgtgcagg
     4741 agcagataaa atcaagacgc agacagacaa aggaaaaggc aacgccgatt tttgcagggc
     4801 ttgtcaagtg tgcggattgt ggttggtcta tgagatttgg aacgaataag acaaataaaa
     4861 cgccttacag ttattatgct tgcagttact acgggcagtt tggcaaaggt aattgttcta
     4921 tgcactacat tcgttatgat gtgctgtatc aagccgtatt ggaacgcttg cagtattggg
     4981 ctaaggcagt acagcaggac gaagaaaagg tattgaacaa gatacagaaa gctggcaatg
     5041 cagagagaat acgggaaaag aagaaaaagg caagtacatt gaagaaagcc gagaacagac
     5101 aaaatgagat tgaccgttta tttgcgaaaa tgtatgaaga tagagcctgt gagaagataa
     5161 cagagcgaaa ttttgtgatg ttgtccagca agtaccaaaa agaacagata gaactggaac
     5221 agcagataac aagcctaaga gaagaactaa gtaaaatgga acaggatatg ataggtgctg
     5281 aaaagtggat tgagttaatc aaggaatatt ccgtaccaaa ggaactgaca gcaccattat
     5341 taaatgccat gatagaaaaa atcctcatac atgaagcaac aacgaatgag gataacgaaa
     5401 gaatacagga aattgagata tattaccgat ttattggaag agttgagtaa tcaacaagag
     5461 taatattttt aactaaggga aaccggcggt gtctatactt gggtcagtcg cacattggga
     5521 aaacgattcg gttttgccgc aatctttttc caatggtttg agataacagt tggctacttg
     5581 accatgctgt atttcttaac tggtgcatta tcctatgcaa cgggtattgg tgctatacaa
     5641 aacaataaat atctgaaatt ggcaatccta ttgattattt tctgggctat tttactctca
     5701 cagttaaaag gaaccaaata cacttcattg attgctcgga tcggttttgt agctggtatc
     5761 ttgttgccag cattagtctt gtttggctta gggatccatt atgtcgcttc aggcgctcca
     5821 ctacaaacga tactgtctat gaaaacattg attcctgatt tttcgaaatt gcctacgctg
     5881 gttgtttttg tttcctttat tttagcatat atgggcgtag aaacatctgc cagtcatgca
     5941 aatgaaatgg aaaaccctaa aaaggattat ccattagcaa tgtttgtatt ggttattctg
     6001 gcaatcattt tagatacttt tggcggattg accgtcgcta ctacagtacc tcaacatggt
     6061 ttgtctctta atacaggggt gattcaatcg ttagaattcc tattaagaca cattgatcca
     6121 aatcttgcat ggattgcgaa aattcttgcg attcttgttt gtttaggtgt catcggagaa
     6181 attgcttctt gggtaacctc tccttcaaaa gcacttcacg tagcagcaga agaaggatta
     6241 cttcctgaat attttgcaaa agaaaacaca cacggtgttc ctgctcattt gatgatcgca
     6301 aatgggatcg ttgcaacaat ctgggcagcc gtctttacat taagcggcgg ggggaataat
     6361 ctttcatttc ttgcagcgat gtcattgact gttgtaatct acctgatgtt gtactttatg
     6421 tttttcgcgg gctatttgaa gctggttttt aaagatgaca gtcttgaaag agcctatcag
     6481 gttccaggcg gaaaagttgt aaaagtaatc gttgcttgtt taggtctttt gacttcactt
     6541 tttgcttttt gtatctcatt tgttccacct gctagtatcg cagctggaca gcatgggatt
     6601 tatgaaacga ttctgattgt ttcttatctt atcgtcttag ttattccatt tacgatctac
     6661 gcatgccgtc ataaattcca gcatgcagaa aaataaaaaa tgaaagctgg ttattcaaat
     6721 ggaagtaaaa gaattagaac aattgattgg aaaaaatttc cactattatc aaacaggaca
     6781 agtggcggat tacattccag cattagctga agtggatccc aaacaattag gtatggcgat
     6841 ttatgatttg gatacaaaac aactgattga gggaggagac gccgatgtgc gctttgccat
     6901 cgaaagcatg tcaaaagttc ccgtattgtt attagcaatc aaagacaacg gaatagaaaa
     6961 agtctttcaa acaatcaaca cggaaccaac cggttttgcc ttcaattcta tcatgaatat
     7021 ggaaatcaat catcgcaaac atccaatgaa tccttttgtt aatgcaggag cgattgcaac
     7081 aacctcgttg attgccggta aagatgcaga tgaaaaattt gagcgaatct tgtcatttat
     7141 gaaagagatc tgtgatgatc ctaatatcac attgaatgaa gaaatctatc attctgaatc
     7201 aagaacaggt gatatcaatc gctctcttgc ttactatatg aaaggtaatc aaatgatcga
     7261 aggagacgta ccggagatac tcgatgctta tttcagacaa tgctccctca atgtcaccgc
     7321 tacaggcata gcaaaattag ctgctgtgct ggcaaataaa ggaattgctc cttggaatgg
     7381 caaacaaatc attactgaag aaagtgctac aatcgtcaaa tcaattatga caactgctgg
     7441 tttgtatgat gaatcaggag agttttccgt ccatgttggt gtacctgcaa aaagcggtgt
     7501 aggcggcggg ttgatggcag ctgtgccaaa tcgttacggc ataggtgtct tcagcccggc
     7561 actagatcca tttggtaaca gcgcagctgg tatccaattg ttgaaagatg tagtcaaaga
     7621 actagacgct gacatctttg aataaccatt cttgaaagag gctggggtaa aagtccaaac
     7681 ggctcaaagc tacctacaat gaggctgaaa ttgtgagctt gtttgacttt tgtcccagtt
     7741 tcttttttta ttggtttttg aaagggcttt tctaaataaa aaggaaacaa aaatacgatt
     7801 tacagatatg attaaataaa atcataagaa aaactggagg cgtgtatcag gaatgctgga
     7861 aaattacatc gagcgaaata tatttagaaa agtgtatctg tgtgaacaat tatttgaatt
     7921 ccaagaaata gacatcgaac aaacagctat ttctctaagg gtgactacac caacgatcct
     7981 tcacgatcta gaaagtctag ccgagtgtct agaatattgt ataaaagaac aagtaagaga
     8041 gaagcataag tataaactcg tattcaaaca tggtattgct ctttctgagc tcacacagtt
     8101 tctgtacggt caatcttatt ttttaaagtt tttaagttat tattttaatg gtatattcac
     8161 gagtacagaa ctggctgatc ttgaatttat ttctttgagt aaagtatata caatcaaaaa
     8221 aatcgtattg gattttttta aagaaaatga ttatttgaaa aataaagaaa tcattatccc
     8281 agaatttgac tcgcggaaca tcttgctagc tttagtacgt tatgtcaatt gggaaggcta
     8341 tgaaaataaa aatccgtatg tagaagaagc ttgtaacgag ttgattgatt atgtagagac
     8401 tcattttttt aaaagaagat attcggaaga tgaaaagtcc tttatcatac gagaaataga
     8461 gatcgcaatc ggaagaaagg catttcccct tggactgaaa gaagaagata agaaaagcgc
     8521 agaaacgaag ccgttatttc aattagtctg tcaaggattg gaagagcaaa aaggaattct
     8581 agacttacaa gaagaagatg tctattatat tttttctttg ttcaacagta gaaattatac
     8641 caatgagaac atggagctgt taagaaaaga tttcgaagtt gtttataaaa attttgttct
     8701 caatgaccta tcactgaatg aattgatttt acagattatt agtatcgctg aaatcgacga
     8761 tgcagaaaat tttattttca agcaatcctt tttacaattt gtccgtacgt tgtgggcaga
     8821 tggacaagta tttttaccgg aaaggatcta tcttctatca gaaaaagaac aacttctgta
     8881 cggaaaaatt gtagaaatat tggaagagtg gaaaaagaaa aatcaaatcc aactaagatg
     8941 gaatgaaaat ttcattcgaa aattcgtcaa aagtctttct ttgatcaaca gtgaacaagc
     9001 ggggagacag gaaatagaaa tttttgttgt ttcagaaagt tcagtcaaac aattttttta
     9061 tcgaacccaa tttcatttgt atgcggaaga aggcatcgct gaaataaatc ctattttatt
     9121 tcgttcatta gaagaattgc ctgatgaatg tctatgtgcg aaaaaaagag tggttttgtg
     9181 cgatgctgct agctatcaaa atggattaga aactgaaaag acaaagatct atccaatctc
     9241 attaaaaggt gcaaacatcc atttgtatca tattgctcag gaacttcacc tattaaaaaa
     9301 acatatttct tgatgagacc ttaattagga gaccttctac aaagcttttt aattgtctgt
     9361 agaaggtttt tatttgagtc ctgattcgtg cgaagatcta cttatataac aagctttctc
     9421 tgaaattttt ttaaaatgct attgacttag agtcaactct aagtagtaga atagataaag
     9481 tgtaacaaat ctattttcat taaaagaaga tagtacggag gagagaatgt attatgagta
     9541 cagtattaca tgtattggat gcaatcaata gccccgcaga tgtcaaaaaa ttatctgttg
     9601 aggaaatgaa acagttggca gaagagatgc gccaaaaaat cttgcaaaag gacagcgatg
     9661 taggtgggca cgttggtccg aatctaggtg tagtagaatt aacgattgcg tttcattatg
     9721 tatttgattc accaaaggat aaaatcgttt gggatgtttc ccatcagtct tatggtcata
     9781 agcttttaac tggtcgaaaa aatggttttg aaaaaggaca ttaccgtgaa gtgagcggat
     9841 attccaacca acatgaaagt aaacatgact attttacagt ggggcacaca tctacatcta
     9901 tcagcttagc aacaggttta gctaaagcga gagatctgaa gaaagaatca ggaaatgtgg
     9961 tagcatttat cggtgacggc tccctaagtg gtggcttagc ttttgaagga ttgaacaatg
    10021 gtggtgtttt acaatccaat ctaatcgtat tagtaaacga taatgagatg tctattgatg
    10081 agaatcatgg cagtttatat aaaaacttag ctgagcttcg tcaaaccaac ggtaaaagca
    10141 gcaataacct atttaaagca atgggatttg attatcgtta cgtggataat ggaaacgatc
    10201 tggaaacggt gatccgttta tttgaagaag taaaagatat tgatcatccg attgtacttc
    10261 acgtccatac ggaaaaaggt cgcggttatc agccggcaat cgataataaa atgaaatatc
    10321 attggcatgt accatttgat ctgtccaccg gggaggcaaa gcaagtaact tctggagaaa
    10381 catttaatat gatcatcatg gattatttgg ataagaaaat gcaaacaagt acagctccta
    10441 ttgccgcagt caatgcagcg ataccaggcg cctttggact gaaaacattc caagaaaaac
    10501 atccagatca ttattttgac gtggggatcg ctgaacagca ctctatcact ttcgcttctg
    10561 ctttggcttc tcagggagta cgtccagtcg tcttccaatc aagtactttc ttgcaacggg
    10621 catatgatca attatcacat gatttagcga tcaacgagaa tccagtgatt attttagtcg
    10681 gtggcggaac gatctcagct agtgaccgaa cacaccaagg aacatttgac attccttact
    10741 tatcaagtat cccgaacatc aaatatcttg ctccagcaac aaaagaggat atgatctcta
    10801 tgctagattg ggcgttcgaa caaacagcgc agcctgtagc aatccgaatt cctacacatg
    10861 gtgtagaaca tggtacagct atcgcaaatg attatagtat tccagcctat caaacagtaa
    10921 ataatggagg aaaagtagca attttagctt taggcggttt tcttgaatta ggaaaagaaa
    10981 cagtaaaagc ttttagaaaa aaaggaatcg aagcaacgtt gatcaatcca ggaacgatca
    11041 cagaaaatga cgtagaaaca ttagataaat taaaagaaac acacgaatta gtcatcacat
    11101 tagaagatgg tagtttgtca ggaggatttg gcgaaaatat cagtcgttat tatggtgatt
    11161 cttcaatgaa cgtattaaat tttggtgcag agaaagaatt cacggatagc gttcctgtag
    11221 ggcagcttta tgaacgttat catttaacac caaaacaaat cgttgaagat agtctgcgtg
    11281 tcttgaatat tcaataattc attatgctca ccatccagat aattgtttat actagtatct
    11341 ggatggtttt ttcattataa aaagcaaagg ggtggatgtt atgtatcagc aagtagaaga
    11401 atatgtcaaa aagttccaag aaaaatatcc tagttatcaa tcagaaaaat taggtttctt
    11461 ttcatttggg gatacagaaa aaatggcaga cgagttgagc gaactagttg taaaaaagat
    11521 aaagactggt acatcaagtg gttacgatct gtataaacaa gagaatgagc ctttaccaaa
    11581 agttggtcaa ttagacgtgg tcctgaatgg aaaaaatgaa ccagtttgta tcatcaaaaa
    11641 tataaatgtg accatcttgc cttttagtga agtcacgaaa gagcatgctt ggaaagaagg
    11701 agaaggggat cggacattag cttattggcg gaaagtccat gctgagtttt ttaaacatgc
    11761 ttataaagaa gcgttgaaca tagaatttac agaagagaag ctggtcgtat acgaagaatt
    11821 tgaagtaatt ttcttttagt aagaaaggtc tgctgaaaga aatagctcat tattgtctcc
    11881 tgaatagaaa agggttacat ttttatatga agctgtattt ttatataaca ttactggagg
    11941 agatattgtg gatacactgg aacaaatcaa aggcggagtc gtcgtttctt gtcaagcttt
    12001 ggaaaacgag ccgctacaca gttcttttat tatggggaga atggctttgg ctgcaaaaga
    12061 aggcggagca gttggtattc gagcaaatac gacagaagat atcaatgaaa tcaagcgaat
    12121 cgttgattta ccggtaatag ggattatcaa aagagattac gatgggtcag aagtgtttat
    12181 tactgcgaca gaaaaagaag tggaagaact tttggagacg caagcagaaa tcattgcact
    12241 ggatgcaact tctcgtaaac gtccgacaga aaaaacaact gctgaacttg tccaaatgat
    12301 tcacgaacac ggtcggttag ctatggcaga tatttcgact tttgaggaag cgcttcaagc
    12361 gcaagaagat ggttttgatc ttttgtctac gactttagca ggctatacag aatattcacg
    12421 aaagacgcag acacctgatt ttgatttatt gaatcaaatc atagaacaag tcgatatccc
    12481 agtaattatg gaaggacata ctgactctcc tgaacaagta gagaaagcat atgaattagg
    12541 tgctttttca gtcgtagttg gatctattat cacccgtccg caattgatca ctaaaagata
    12601 tgtcgaagcc gcaagtaaag caaataacta aaaaacacgc acggcacaga ttcttgacaa
    12661 aaaagaaaaa ttgatagata gtttaaagca gattctaaga agatgaaaaa agcattactt
    12721 ttcattttgt cttcaagatc tgctttttaa cgaattttat agaaagaagg cgaaaccatg
    12781 tttgaacgga gtccttcaga attgacagaa cgtgaaaatt acaagttgct gattggttca
    12841 gtcatcccta gaccagtagc agtagtaacg actaaatcag caaaagatat agtgaatatc
    12901 gcacccttca gttacttcaa tatcgtcagt tcgaatcgac ccatcttatc cttggcgatc
    12961 cagagaaaac aagatgcagt aaaagacaca gcaaagaata ttcttgagac aagagaagct
    13021 gttatccata tccttgatga ggacaacgta gaagaagtaa ataaaacagc agccaatctg
    13081 cctaatgagg aaagcgaact gtacctgact gacttgactt taactgattc agtaatcatt
    13141 tcagtaccag cattgacaga agcaaaagtt cgttttgaaa catccttata tgaacatgtc
    13201 gagatacgga ataaaaataa agtaacagct gatttatttt tgctagaagt acagaaatat
    13261 catttaaatg aaacagtcta tgatgataag acaggaagga tcgatccaag aaaattgaat
    13321 gctgtaagcc gtttggcagg aaatgattat gcagcaatag gagaaatttt cacgattgca
    13381 cgtcccgaat aaaaaggagc actagttatg ttaaaaataa gctataaacc atcgacagat
    13441 tcaaaagaaa tgaaaaaaga atatgaaaca gtgaatgatt ttctgcaagg gcagtactta
    13501 gaagttccgc cattgcaaga ccattttgtc gttactactg ttactttaga tggtaaggaa
    13561 atagaaatgc cagatcaaac gattagcgga ttgtttaatt actttaacaa ataaatattt
    13621 attccgaaaa gtgaaaaaac atataaaaaa acacataaaa ctaaaaaatt atcaaagaat
    13681 cctggtgcaa ctgaaagaat ttgcaacggg attctttcaa cttatttgtt atttttttct
    13741 aaaaaatatt aaagattgtt aaatttcact atttcatttc aaattccatt gcctaatcat
    13801 taggaaaagt ctatcatgaa ctcgtaaata gaatatagta ataaccttgt taggtgaggc
    13861 tcctatacaa acataggctg ttgcccagaa acgtcgaaag tcgccaatgg gtaaaacaga
    13921 aactgttgga ttaaggcttt ttccaatagc taagaatgtt ttctttacgt tgtatagtgc
    13981 taaaactcgg acgatgagat gatgctaggt cagaattctt gttcgaaaag gtttctgacc
    14041 ttttctttat atttttgtga ctgacttatt tgatgagaca aactcgctcc tcacctgaca
    14101 agtggattgc tgtgtgagga ggagatgcgg tgaaaaaaga tcgattggtg gattttgggt
    14161 ccttatttga ctttgtccat gatcggtctt ttagaagtat acagtgccag ttcttatcgt
    14221 ttattgcaag ccgatgaaaa tacaaaaagt ctattattgc gtcaactaat tttcatattt
    14281 ttgagttgga gcgtgatctt cttagctcgt tcagtcaaac tacactattt acttcaccct
    14341 aaaatagcag gatacggttt agccttatcg attttctttt tagtattagt aagaataggg
    14401 atattcggtg tcactgtcaa cggcgcacaa cgttggatct ctctgtttgg cattcaattc
    14461 cagccttctg aactggcaaa tctttttttg attttttatt taagctggtt ttttcgtgac
    14521 ggaaatagta gcccaaaaga tctaaaaaaa ccattcctga ttacagtagg tataactttt
    14581 ctgattttat ttcagccaaa gattgctgga gcattgatga tcctttcgat tgcgtgggtc
    14641 atattttggg cagcggcggt tccatttaaa aaagggatct atctaatcgt tactttttct
    14701 gcattgctga ttggagcagc aggcggggta ttatatttag gaaataaagg ttggcttcca
    14761 cagatgttta atcatgctta tgaaaggatc gcgactttaa gagattcttt tatcgatagt
    14821 catggtgcgg gctatcaaat gacccattcg ttttatgcac tatataatgg tggcatattt
    14881 gggcggggat taggaaatag tatcacaaaa aaagggtatc ttccagagac agaaacagat
    14941 tttatttttt cgattattac agaagagctt gggctaatcg gtgcattgtg tgtgcttttc
    15001 ttattgttct cattatgcat gagaatcttt tgtttaagca gtcgctgtaa aaatcagcaa
    15061 gctggattgt ttcttttagg attcggaact ttgctattcg ttcagacgat aatgaacgtg
    15121 ggaagtatcg cggggctgat gccaatgact ggtgtgccat tgccatttgt tagctacgga
    15181 ggaacaagtt atctgatcct ttctttagga atcggaatca cgttgaatat ttcttcaaag
    15241 atacaggccg aagagttggc gctctatcga cctgaaaaac aataaagaga aactgagaaa
    15301 ctctcagttt gaattatcat aaagtacaat cttttatttt tcaagcgaga ggttctttta
    15361 gaaagaggaa ttcgcaatga aagtatctca aaaaatcata ttatctattt tgttactgct
    15421 tacattagta gttggctatt tttcgattga gtttatccat ggattttcgt ctgcaaaaca
    15481 gagttcaact gtgaaaaagg tagagccgaa aagtatccct accacactaa atgtggcttt
    15541 gattggttca gatgctcgtt cgaaagacga aaacggtcgc tcggattcac ttctggttgc
    15601 acaatacaac cagaaaacac aacaagcaaa actgatctct atcatgagag attcatatgt
    15661 cgatattcca ggttacggaa tgaataagat aaatgcagct tactcttatg ggggagttga
    15721 tttattaaat caaacattaa aggaaaattt caaatttgaa gctccctatt atgcaagtat
    15781 cacatttcaa gattttatcg attgcgttaa tgaactgttt cctggtggag tcaagatcga
    15841 tgcagaaaaa tctctagatt tagatggcgt atatataaac aaaggagagc aagtgatgga
    15901 tggtaatact ttactccagt atgctcgatt tcgggaagac aaagaaggag acttcgggag
    15961 gatcagaagg cagcagcaag tgatcaaagc tatctctcag cagctgaaag acgtaacttc
    16021 aatattcaag ctacccaaag cggttgggaa attacttggt agtatccaaa cgaaccttcc
    16081 agaaagtgtg cttctggatt gtggtatgga tttcctcaaa gacatatgtt agaataaaca
    16141 ggtataaata gtgaaataaa ggaatgacaa gcaagagaag gaggaaaaaa tgaaaagaag
    16201 tgacaagcac ggcaaaaatc gaacaggcgc ttatattgcc ggcgcagtga tcttaatagc
    16261 agctgcgggt ggcggttatt tttactacca gcactatcaa gaaacccaag cagtagaagc
    16321 tggagaaaag acagttgagc aatttgtcca agctttgaac aagggagatt ataacaaagc
    16381 tgcagaaatg acatcaaaaa aggcggcaaa taaaagtgca ttatctgaaa aagagatctt
    16441 agataaatac cagaatatat acggtgctgc tgatgtcaaa ggacttcaga tatcgaatct
    16501 aaaagtagat aaaaaagacg attctactta cagtttttca tataaagcaa agatgaatac
    16561 ctcattaggt gaattgaaag atctttctta taaaggaaca ttagacagaa atgatggtca
    16621 aaccacgatc aactggcaac ccaacttggt ttttccagaa atggaaggaa atgacaaagt
    16681 aagcctgacc acacaagaag cagcaagagg gaatattata gatcgaaatg gtgaaccatt
    16741 agcaacaaca ggcaaactaa aacaattagg agtcgttcca agtaaacttg gagatggagg
    16801 cgaaaaaaca gccaatatca aagcgattgc ttcctccttc gacttaacag aagatgctat
    16861 caatcaggcg atttcacaaa gctgggtaca acccgattac tttgtcccat tgaaaatcat
    16921 tgatggagca acgccagaac ttccagctgg agctaccatt caagaagtag acggcagata
    16981 ttatcctttg ggtgaagcag ctgctcagct gattggttac gtgggagata tcacagcaga
    17041 agatattgat aagaatccag aattaagcag taatggaaaa atcggtcgct caggtttgga
    17101 gatggctttt gataaggatc ttcgtgggac aacaggtggg aaattaagca ttacagatgc
    17161 agacggtgta gagaaaaaag ttctgatcga acatgaagtt caaaatggca aagatatcaa
    17221 attgacaatc gatgcgaagg cacaaaaaac agcttttgac agtctaggag gaaaagctgg
    17281 atcaactgtt gcgacaacgc caaaaaccgg tgatcttctt gcgcttgcta gctctccaag
    17341 ctatgatcca aacaaaatga caaacgggat ctcacaagaa gattacaaag cttatgaaga
    17401 aaatcctgaa catccattca tcagccgatt tgcgacaggt tatgctcctg gatctacgtt
    17461 taaaatgatt acagcagcaa tcggtctcga caacggcact atcgatccaa atgaagtgtt
    17521 gacgatcaac gggcttaaat ggcaaaaaga cagttcttgg ggatcttatc aagcaacgcg
    17581 tgtcagtagt gatgtatcac aagtagactt aaaaactgct ttgatctatt ccgataatat
    17641 atatgcggca caagaaacgt tgaaaatggg tgagaaaaaa tttcgtacag gcttagataa
    17701 attcattttt ggtgaagacc ttgatttgcc aattagtatg aatccagcac aaatttctaa
    17761 tgaagatagc tttaactcag atatcttgct agctgatact ggatatggac agggcgaact
    17821 tctaattact cctatccagc aagcagcaat gtattctgtt tttaccaaca atggcacact
    17881 tgtctatcct aaattgattg cagataaaga gacaaaagat aagaagaatg tcatcagcga
    17941 aacagcagta caaacgatcg tgccagatct gagagaagtt gtgcaagatg taaatggtac
    18001 agcacattct ctttctgctt tagggattcc gttggcagcg aaaactggta cagcggaaat
    18061 caaagaaaaa caggatgtaa aggggcaaga gaacagtttc ttgtttgctt tcaatcttga
    18121 taaccaaggt tatatgatgg tcagcatgtt ggaaaataaa gaagatgatg attcagcaac
    18181 caaacgagca tcggaactat tgcaatacct caaccaaaat tatcaataaa aaattgcttt
    18241 ttaagtacag taaaaggact gtggcgttat cgtcacagtc cttttttcaa ataaacggtt
    18301 ttcacgatgt ctctttcaca acttcttttt gatttactca cacttcataa actttttttg
    18361 taaaatattt gagaacaagt ggagaaataa gtgtacttaa taaaatcacg ataactaaag
    18421 gtgaatagta ctgtggttcg atcaattgac tttgctgtcc aatttgtaaa atgatcaaag
    18481 ccatttctcc acgcgaaatc ataccagcac cgaccatcaa tgaactatgt tgagaaaaac
    18541 cggcaatctg agcaccaaaa taacctccaa gcaatttggt caaaatcgct acaagcgtca
    18601 atatagaaat aaagagtagt tgttcactaa agtcagaaaa atcaacttct aatcctacac
    18661 tgacaaaaaa tactggtata aatactgcat atcctaaagc ctctacattg tggtatactt
    18721 cttctttaac ctttgtctga ctgacagcta tcccagcaaa aaaagcacca atgacagagc
    18781 taagcccaac aagatccgct acgtaagaca ttcctaaaca aataactagc gatacgatga
    18841 taatagaaga atttgcgtac actttttctg caaatgaaag taggaaaggc gcaatccatt
    18901 tgactaataa gaaaataaaa acaaaataaa ttagctgttc aatcaaaaca agcggtaaag
    18961 aagcattgct agaagcttcc cccgttagaa aagataggct gaaacttaga actaaaacga
    19021 ctaaaatgtc atctaccaca gaagcaccca aaattgttga tccttctttt gtattcacta
    19081 cgtttagttc ttttaatact tctactgaaa tactgacaga agttgccgca agaataatcc
    19141 caaagaaaat cgcttcattt cccgaaactt ggaatgcttt gcctgtcaac cagccaaaaa
    19201 caaccgggaa gatgatccca agcaaagcaa cgaacatacc aggtcggaaa tattttttca
    19261 aaagagataa atcactttcc agaccagcta aaaacattaa taagatcaca ccaatctccg
    19321 aaaagtcatg gactagaata tcaggtttga cccaattcaa tccagcttgg cctagcaaaa
    19381 caccaacaag taattgtcca atcactgctg ggatacctaa tctccgcgag atatgtgagc
    19441 caatcgttgt tgaaatcaat atcaggcata aaataccaat aaattccatt ctcatcattc
    19501 ctttgtataa aatatagccc gaacttagca taacatacat tattgaaaaa ataggaaaat
    19561 atactcacaa aaaaaccaac aatccaaaag atttttttgg attgttggtt tttatacggt
    19621 ctattttaat aactaaaaat gattaacgta tagtcatttt tcttattctg atgaaattga
    19681 taatctcctg gatgcccatg aaaatcaaaa taaagccgaa tatttggaat agaagcatca
    19741 aacttttgaa tggattgaac aataagacga atccgccaat caacaacaga atactactat
    19801 aaacactcca acttttagct tttgtattca tgctgaagaa caactggaac aagctgttga
    19861 tgataacagc taatccaaga agaatcggca ggatagaaac gattgcaggt gcaaaagcat
    19921 agacgatcag agctaagatc aggaaaaaaa gaccactgaa cagaccgata ctccaagaac
    19981 ctgttttcct tttaaaacgg taagcctcaa aaagattcaa caacccaagc aaaacaagat
    20041 aggcggtgat cagataaccg ataaaatgaa aaacagcaga agggtttagc acaatcgcaa
    20101 tcccagaaat aatataaaca gcagctctta ataaggcgta tttttgaata ttttgtttaa
    20161 tcgtctcaaa catataagaa aacctccttt gacttatttt aacgtgaact agtcatttta
    20221 ccaaattaaa tgttacaaaa tgagcgaagg agaagaatat ctctggaaaa atcaacttct
    20281 gaggatcttt tccatttttt tgccttttgc tagctcatcg atcattttat ctaaataacg
    20341 aatttctttc atcaagttat cctcgatgtc ttctactcgt acaccacaaa caacacctgt
    20401 aatcaaggaa cgtgaaggat tggggacagg ggcttccgca aaaaattgtt cgaaagtcac
    20461 ttgtttttgt agttgatatt ctagttctgt ttgggaataa cccgtcagcc aacaaatgat
    20521 ttcatctact tcctctttta tacgatcttt tctctccgct tttgctacat acataggata
    20581 aacgcttgca aaacttgtcg taaaaatacg attttgcgcc ataatcagtc acctttcttt
    20641 ttttcaaatt atttactact tatagtatat aagaaaaaga gaagaaaaag cgattattca
    20701 aaaaaacaaa aaggattgtg acaaaattct tgtcaccatc cttttttccg ctctctgact
    20761 ttttacaatc ttcatagctc aagtgcgcat cagataatcg aatgctccta aagctgcagt
    20821 agctcctgaa cccattgaaa caatgatttg tttgtatgga ctatctgtac aatcaccagc
    20881 tgcgaaaacg ccggggatat tcgttgcgcc atttttctcg gtcactattt catttcttgg
    20941 attcatttcg atcgttccat ctagccaatc cgtattaggg agtaagccga ctagaatgaa
    21001 tactcctgaa acagctagtg tatgcatctc catcgtacgg cgatcgatat aggagagaga
    21061 ttctacctga ttcgtaccgc tgatttcttt cgtcgctgca tgtttgataa cagtgacatt
    21121 cgcaagagag actagtttat cctgtaagat ttgatctgct tttaattctg gtaagaattc
    21181 tagaacatat acatgttttg ctaacccagc caaatcgatt gctgcttcaa caccagaatt
    21241 accaccaccg ataacagcaa tctcttttcc tttaaataaa gggccgtcac agtgtgggca
    21301 ataagcaatt cctttatttt taaactcttt ttcgcctggt acattgatag aacgccagtg
    21361 cgcacctgtc gcgatgatgg cggtttttgc tttaagtgat ataccgttag ctaagccaat
    21421 attaataaaa tgttgctctt tatttaagga aacggcctgt tgattcatca taatgtcgat
    21481 agggtaacta cgcacatgac tttctacttg ctgcatcaat tttggacctt ctgtgtaaga
    21541 ggtaccaatc atattttcaa tccctagtgt ttcaacgact tgtccgccga aagaatcagc
    21601 gacaagtcct gtacggattc ctttacgtgc tgcatagata gctgcactac tagctgctgg
    21661 tccaccgcca atcactaata catcataagg ttctttttca aacaactctt cttcaatcaa
    21721 agggccagtg attttttcaa caatttgttc gatcgtcatg cgtccgctac taaattcttc
    21781 tccatttaaa aacacagttg gcactgccat gattttttta tcatcgattt ctttttggaa
    21841 catcccacct tcaatcatcg tatgagtaat atttggattt aatacagcta atatattcaa
    21901 tgcctgtaca acgtcggggc aattatgaca agtcaagcta acataactgt caaaatgcaa
    21961 tgtttgtgtt atttttttga ttttaagact caaagattca ctgattttcg gtgcccgtcc
    22021 actaatttgt aacatagcca gtaaaaaaga ggaaaattca tgaccgaggg ggataccagc
    22081 aaaaacgatt ccactttctt tgccaactgg attgatagaa aaactaggtg tgcgcatcga
    22141 atctcttttt tcccaagaga ttttaggaga catttctgct atttccttcg taaattcagc
    22201 tagtttttga ctattttcac tattatccaa actaacagaa aaaacaatag gagattcaat
    22261 cagtgctaaa tattgttcga gattttgttt agtttctttg tctaacatat ccaccaccta
    22321 aatctttcca acaagatcaa aacttggttt caatgtttcg ccactttctt tccattttgc
    22381 cggacaaact tcacctgggt gggtacgtac atattgagca gcgcgaatct tatcaatcag
    22441 tgcactagcg tcacgaccga ttccatctgc attgatttcc atagcttgaa cgattccgtc
    22501 tggatcaata ataaatgttc ctcgctgagc taatccttgc tcttcatcca aaacatcaaa
    22561 aatacgagaa atctgatgag acggatcacc gatcatcacg tattcgattt taccgatagc
    22621 atctgatgta tcatgccatg ctttgtgagt aaagtgggta tcggtagagc aagaaaagac
    22681 ttcaacacct aaagatttca atgtactgta ttgttcttgt aagtcttcta attcagtagg
    22741 acaaacaaaa gtaaaatcag ctggatagaa gcaaagaata ctccagttcc ccataagatc
    22801 tttttcactg acttctaaaa attctccttg atgataagca tgtgcattaa attcttcgat
    22861 ttttttccca atcaatgaca tagtaaaacc tccaataata attatttatt tataattatt
    22921 gtaatttagt tggagttgca aagtcaatgg atattttgtt atttaataaa gattactcaa
    22981 aaacgaaggt taattataaa aatagaagag gtcgcaagaa aatgctactg ctggaaatac
    23041 tcgggataac gttgagtaac agtttcctgt tctttcagca tcatgtgtaa gtgcaatccc
    23101 gtttgaaatc gagcttcttc tggtttttta tcaactaaag ccttgagtaa atcataatgt
    23161 tgttccatga tcgtttccca tggaagcccc tgaatggtca ctcgtaacca gcggaaacgt
    23221 tctaaatggg tattatggtc atctaaccag ttccaaactt cttttcttcc tgcaatttcg
    23281 aaacaagctt catgaaataa attatctgca cggaaaaaag cgcgttcgtc atttattttg
    23341 actgcttttt cttgttcttc taaaatcgtt tctagcacta ttcgtgtttg tgtgtccaat
    23401 ttggaacaac attcaatcat gatttgctct tccaactgtt ctcggacaaa gcgagcattt
    23461 tctgcagaaa caagattaat ttttgaaaca tacgtaccac tttgaggtat cgtatacacg
    23521 agttcttgct gacgtagttg aatcaatgct tcgcgaatgg gagtccggcc gatagcaaga
    23581 tgttcctcca gtgccttttc agatattttc cttccaggtt ctaagtcagc atagataatt
    23641 tgtttacgaa tcgcttgata agcttgagat tgcaagctgc ttttcatctt tattcctcca
    23701 aaatcatttc tatgaacgta gtataacaga agaggtctga aaaacaatct ggtatgtcac
    23761 attaactagt aaatttgtat aaatagtaaa attttggttg acatagcgat ttttattgca
    23821 taaaattcac ttgaatacaa aaactaatat gttagtttag gaggggaaaa atgattgatt
    23881 taaacaactg gaggaacttt atccctgaat tatctcaaaa agaagtagaa atacctcatt
    23941 ttgaccgtgc atcattaaga gaaaatggac aaaaaaatcc tgtctggatc catttcggtg
    24001 gcggaaatct ttatcgagga tttcatgcag aaatcgctca acgtttagcg gatctaggcg
    24061 aattgtccac tggtgtcatt gtttgtgaaa cattcgatga agaaataatt gaaagggctt
    24121 accaagattt tgatgatttg attcttgaag tggtcatgcc aagcagtgga gagttcgtca
    24181 aacgtatatt agctgcgaca gctgacagtc tgtattgtca tccagattgt tctgaacact
    24241 ttgataagat gattcgacac tttgaaaatc ctagtttaca attagttact tttgctatta
    24301 ctgaaaaagg ttatcgattg aaagatacaa ataataaata tataggaccg attgcagtcg
    24361 atataaaaaa tggtccaaag aagccatcac atacaatgag tattcttgct gcgctacttt
    24421 ataaacgttt tttatctgga gcttggccga ttgcacttgt ctctacagat aatttttcac
    24481 aaaacggtag tcaattacgt acttctttgc taacgatcgc aaaagaatgg caaaaggaag
    24541 gttatgtaca aaatacattt atccaatact tatcgaatga agaaattgtt gcgtttccat
    24601 ggacgatgat tgatcgtatc accccgaatc cttccttgtc agttagcaaa atgttggcaa
    24661 aagagggctt ttcaaatatg tcgattttcc aaaccgcaaa aggaaataat attgctccat
    24721 ttgtcaatac agaggacatc cattatttaa tcatggaaga tcattttccc aatggacgtc
    24781 cgaaattcga aaaagctgga gtattcttga ctgatcgtgc aacggtagat aaagcagata
    24841 cgatgaaggt gacgacttgc cttaatcctt tgcatacgtc tttagctatt atgggttgtt
    24901 tactggggta taggagcata gctgaggaga tgaaagatcc agatttatcg ctgctagttt
    24961 ctgaaatcgg gtacaatgaa ggcctcccag ttgtggagtc tccaggtatc attgatccaa
    25021 agatctttct ggaagaagta attactgtgc gcttttctaa tccaaatatt cctgatatgc
    25081 ctcagcgaat cgccacggat acctctcaaa aggtggcgat tcgttatggt gagacgatta
    25141 aaaaatatat ggaaatagaa gttaaagacc cgcagacact tgtattcata ccattgactc
    25201 tcgctgcttg gcttcgatac cttattggca ttgatgatca aggaaaacca ttcatcccga
    25261 gtccagaccc actattgaca gactttcagc ataagctttc cgatattcaa ctgggtacgc
    25321 aagatacatc acatattcat gagaaaatgg ctcctatttt gtcaaataaa gaaatttttg
    25381 gtgtagatct ttaccaagca gagcttggca gaaaaataga agaaatgttc atcaagatgg
    25441 tagcaggacc gaatgccgta cgaaaaacat tacaggatta cttacaagaa catggaggaa
    25501 atttatcatg gaaatgactt ttagatggta cggatcacag gaagaaaaga ttaccttaga
    25561 tgaaatcaga cagattcctg cggtcaaagg gattgtaggc acattgatgg atataccagt
    25621 aggagaggta tggcctaggg agaggatcaa agaactaaaa gaagaaattg agtctcgtgg
    25681 gttaacacta aaagtaattg aaagcgtcaa tgtgcatgat gccattaaaa ttggattacc
    25741 ttcccgtgag cagtatatcg aaaactataa gcagacaatc cgaaacttgg cagaatatgg
    25801 aatagaagtc atctgctata attttatgcc tgtatttgac tgggtgaaat ctgaccttga
    25861 ctaccgctta gaagacggct catccacgtt agcttttata tctgatgata ttccagatga
    25921 tccaagagaa atcgtcgcgc gcatcgaaca ttccagcaat gaatttgaat tgccgggatg
    25981 ggaacctgag cgcttagctc atatcaaatc cttgcttgaa gcctatgcat cagtagacga
    26041 agaaaaactt cgagaaaatt ttgcctattt tctccaatca atcatcccaa catgtgaaga
    26101 ggtaggggtc aaaatggctg tacatccaga tgatccgcca tactcgattt ttggattacc
    26161 tcgaatcgta aagaatcgag aagatttaga ttggttgtgc cgcatagtag acagccctta
    26221 taacggaatc actctttgta cagggagtat tgcagaagat ccagaaaatg atgtatatgg
    26281 gattttagca gaattttgtc aaagagatcg aatcccattt gcacatgttc gaaatattaa
    26341 atttttagct aatcgagatt tctatgaggc accgcataaa tccgaatacg gttcattaga
    26401 catgtaccgc atactaaaaa caatgtatga caacggattt aacggataca tccgtcctga
    26461 tcatggaaga atgatctgga atgagacagg aagaccagga tacggcttat atgatcgagc
    26521 attaggtgct gtttatctaa atggattatg ggaagcaatc gaaaaaggaa acaaaagtaa
    26581 agataattag tgaaaaaaac tgtaatttta ttatgtaaat aagaaggatg ttcaaattct
    26641 t
//
LOCUS       DACCYH010000033        25765 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.033, whole genome shotgun sequence.
ACCESSION   DACCYH010000033 DACCYH010000000
VERSION     DACCYH010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.033"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..87)
                     /locus_tag="IVY85_001330"
     CDS             complement(<1..87)
                     /locus_tag="IVY85_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HAP8044355.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKI"
     gene            complement(100..510)
                     /locus_tag="IVY85_001331"
     CDS             complement(100..510)
                     /locus_tag="IVY85_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8044356.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            785..997
                     /locus_tag="IVY85_001332"
     CDS             785..997
                     /locus_tag="IVY85_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8044357.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1000..1212
                     /locus_tag="IVY85_001333"
     CDS             1000..1212
                     /locus_tag="IVY85_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044358.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            complement(1504..1728)
                     /locus_tag="IVY85_001334"
     CDS             complement(1504..1728)
                     /locus_tag="IVY85_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP8044359.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            1806..1994
                     /locus_tag="IVY85_001335"
     CDS             1806..1994
                     /locus_tag="IVY85_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044360.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2320..2655
                     /locus_tag="IVY85_001336"
     CDS             2320..2655
                     /locus_tag="IVY85_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044361.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            2888..3829
                     /locus_tag="IVY85_001337"
     CDS             2888..3829
                     /locus_tag="IVY85_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP8044362.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            3831..4721
                     /locus_tag="IVY85_001338"
     CDS             3831..4721
                     /locus_tag="IVY85_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAP8044363.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            4764..5564
                     /locus_tag="IVY85_001339"
     CDS             4764..5564
                     /locus_tag="IVY85_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8044364.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            5579..6430
                     /locus_tag="IVY85_001340"
     CDS             5579..6430
                     /locus_tag="IVY85_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8044365.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            6631..7092
                     /locus_tag="IVY85_001341"
     CDS             6631..7092
                     /locus_tag="IVY85_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010781228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8044366.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7118..7570
                     /locus_tag="IVY85_001342"
     CDS             7118..7570
                     /locus_tag="IVY85_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044367.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            7676..7870
                     /locus_tag="IVY85_001343"
     CDS             7676..7870
                     /locus_tag="IVY85_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044368.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNGFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            7901..8287
                     /locus_tag="IVY85_001344"
     CDS             7901..8287
                     /locus_tag="IVY85_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044369.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQKIVLEWLKSETILTREAPILSVNAFSDKNLLGKLPDKVRKAY
                     KLLACKQEYEVLSAFAQWGLEQEEAE"
     gene            8284..8487
                     /locus_tag="IVY85_001345"
     CDS             8284..8487
                     /locus_tag="IVY85_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAP8044370.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            8484..8780
                     /locus_tag="IVY85_001346"
     CDS             8484..8780
                     /locus_tag="IVY85_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044371.1"
                     /translation="MKIKDGFYASSHGIGGLMLDMPIKNPKTRQKSKFKIGDMVRCKA
                     EEFIYPFRGYVEHVYNHSAIIHIENTMECDKWLAKSKDNLAVARLVDMELINDK"
     gene            8856..9272
                     /locus_tag="IVY85_001347"
     CDS             8856..9272
                     /locus_tag="IVY85_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP8044372.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            10080..10310
                     /locus_tag="IVY85_001348"
     CDS             10080..10310
                     /locus_tag="IVY85_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044373.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIVEMTREAT
                     PKEIDKCKLLYCGHGFYDEPNIQNNINKNLRD"
     gene            10469..11302
                     /locus_tag="IVY85_001349"
     CDS             10469..11302
                     /locus_tag="IVY85_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HAP8044374.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPKRNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11295..12710
                     /locus_tag="IVY85_001350"
     CDS             11295..12710
                     /locus_tag="IVY85_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase B"
                     /protein_id="HAP8044375.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            12722..14251
                     /locus_tag="IVY85_001351"
     CDS             12722..14251
                     /locus_tag="IVY85_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8044376.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEYMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKKLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14343..15221
                     /locus_tag="IVY85_001352"
     CDS             14343..15221
                     /locus_tag="IVY85_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAP8044377.1"
                     /translation="MMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15223..15540
                     /locus_tag="IVY85_001353"
     CDS             15223..15540
                     /locus_tag="IVY85_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044378.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            15589..16290
                     /locus_tag="IVY85_001354"
     CDS             15589..16290
                     /locus_tag="IVY85_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAP8044379.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMSMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            16305..17195
                     /locus_tag="IVY85_001355"
     CDS             16305..17195
                     /locus_tag="IVY85_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAP8044380.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17218..17433
                     /locus_tag="IVY85_001356"
     CDS             17218..17433
                     /locus_tag="IVY85_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044381.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            17445..17786
                     /locus_tag="IVY85_001357"
     CDS             17445..17786
                     /locus_tag="IVY85_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044382.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            17786..18157
                     /locus_tag="IVY85_001358"
     CDS             17786..18157
                     /locus_tag="IVY85_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044383.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18150..18545
                     /locus_tag="IVY85_001359"
     CDS             18150..18545
                     /locus_tag="IVY85_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP8044384.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            18547..18924
                     /locus_tag="IVY85_001360"
     CDS             18547..18924
                     /locus_tag="IVY85_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044385.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            18925..19533
                     /locus_tag="IVY85_001361"
     CDS             18925..19533
                     /locus_tag="IVY85_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8044386.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            19533..19874
                     /locus_tag="IVY85_001362"
     CDS             19533..19874
                     /locus_tag="IVY85_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044387.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGPVVEEAAEVMEG
                     MQNAVDGKSVSEALSRAYSFIADILFEGQFTGEDYRQGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20116..22905
                     /locus_tag="IVY85_001363"
     CDS             20116..22905
                     /locus_tag="IVY85_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAP8044388.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            22895..23635
                     /locus_tag="IVY85_001364"
     CDS             22895..23635
                     /locus_tag="IVY85_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8044389.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            23632..>25765
                     /locus_tag="IVY85_001365"
     CDS             23632..>25765
                     /locus_tag="IVY85_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044390.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWM"
BASE COUNT         9377 a         3665 c         5309 g         7414 t
ORIGIN      
        1 tattttatat ggattaatcg catcagatcc aaactctaca aataattttt ctatttcttc
       61 taatatccag tatttgttta aatgcatacc aaagcccacc tattgacgat attttttagg
      121 agtgaatttt ttctttgctt taactttggt taaggcaagt gcaagttgaa tggcatcgta
      181 taatttttcc ttatcttcat cgtctaaagg ttcaccataa taatttgttt cagcatctga
      241 attcaagcct tcaagtaatt tttcagcttg taaagcaata tcttgtttat ctttttctgt
      301 taaatcataa taatggcgtt tatcagtacg tcctaaaaga taatcagttg atacattaaa
      361 gtaatctgca actttttgta ttttatctcc agatggagta cttttattcc atctagacat
      421 gcttccattt ccgaaattta actttctttc aagttctgcc aacgtcattg aacgttcttt
      481 taataagatt tttatacggt ctaatagcat aggatatgca ttcctttcaa aataccgaca
      541 aaaaaagtat ttttctgcaa attatctatt tacaaataga gaatttgcag ttatactagt
      601 tttgtaagca agatatttac ggcatacaaa gactaaaagc aataaactaa tagtctcccc
      661 agacatcagt aagctcgctt tttgtgcgct gtattttgta tgcttacata atagcatatt
      721 tgcagttttt gtcaaactta ttagacaaaa atattagaaa atattcgata agggggtatg
      781 aagcatgact ttaaaacagc aagtattaat tgctttagta aagaaaggtt ggagtcaaag
      841 agagttagct cgacgaatgg gtatatcaat tacttattta cgagatatct tcatcgaaaa
      901 aagaaaacct aaggaacgtt taaaacaaat cgaagaactt ttggatatca agttagaagt
      961 agattctaat gatcagccat cagaacagga ggcgtaaaaa tggtacaacg tttgagcaaa
     1021 gcggagttta aagagaaata taagtattct gaaagtactt atcaaagaag aatgaaggaa
     1081 tttaaaaaat cacgttttag tgaggggtat aacgcagtta cttccaaaga aataacaatt
     1141 gatgtcgatt tatatgaaca gtttagaaca tggcgttcta aaaatcgatt tagagttaga
     1201 gctgtttgtt aatagctaaa aaatctaaat tcaccacttc tgaaaagagg agttgctgct
     1261 ctcctttcaa gtttgccaaa gtccacgttt tttcattttg gcaactcctc ttcttggagg
     1321 tggtgacggc gagagacgaa ggagggatga catgaaggaa gaatcaaaaa tagggatttt
     1381 gaatgagaac aattacacaa gattaattat tgaaacagaa gaagggaaaa aagtagcaga
     1441 agttacattg accgatgcaa ctcctgctga cggttatcgt attcgattaa ctcctaaata
     1501 tgattaacct tttggtgggt gtggatcgtt tccgtgacta tcacggcttt gaatttttcc
     1561 atctctgcca tgaataaata gctcagatcc ttgattttta gaaatctctc tagcgatttt
     1621 tactgcttct gattttgtat tagtatgtgc agtcgcttta gaatttccag cacctttgac
     1681 attccaacca ccattggaat taggtacaac atgttgattt tttcccatag ttttcacctc
     1741 catttctata gaaatatttt atcaaataga aatggagaaa ttcaataaac gaaaggagaa
     1801 gtgatatggc atataccttg caacaagaac atcaaattct cggtttgatt aaacaacgca
     1861 ggaaacaatt acaagatgac cgtgcagcgc ttagaaaagc cgatgagcta tcagataggc
     1921 aagctgaact aattgcttct gaacttgagg atttgagaat gctagaaata aaaaataggg
     1981 agattagatt atgaagaaga cagacacact ttttatagga ttcattttgg ggttattagt
     2041 gattgtagcg caccaaagta ttatcggggg aagcttgttc gcagcattga tggttttaat
     2101 caatctgctt gattcaaaag aaaggagcaa ctatggcacg agaagaagcg ctaaaaatcg
     2161 gtaaagtgat tgctgataat cggtggggaa atagccgccc tattatttta agcaagcaac
     2221 atatcgaaaa gcaaaaagca tggcaacaaa taaaaaagtg actctgccag ccagcaaaga
     2281 gtcacataca aatttataaa actaggagaa ttatatcaca tgaataagaa aattgaaaat
     2341 cttatcgaag aattgaaacg tgaatgccaa aagcaaggtg tttctattat ttgtacagcc
     2401 caaaaagaag gggaacttaa aagtctcgtt tatggtgaga caacagaaat tttactttgt
     2461 cttgctatgc aagaagaaca tttagatgaa aatctcccat tgtcagcaca tattatgcgt
     2521 agaattgctg tggatgctta cgaacaagcg aaaaatgaag aagaaaatca accttcaaat
     2581 catacttttg ttattaataa caaggaagat ttagctgatg tgatgactcg tattctcaag
     2641 ggggaatttc aatgatgaat gaaaaaacaa ttgatctact cattatttta tttttcacgt
     2701 tagtgattgt agcgaaaagt tatgtaatct ttggcttgtt attcgcaatt accatgcttg
     2761 tgctttgcgt gttagcaagc agaaaagagg ttaaacatga acgagttagc aaatcttgac
     2821 aactatttaa ctgatcctga ttatgctaaa ccaccatatg aagcaccaat tgatgaggag
     2881 gatgaagatg agtaaatcta ccttagaaat gagccatcaa gaatggcttg aagaccgtaa
     2941 gaaaggcatc ggaggttcgg atgttggaac aattttagga ttgaataaat ggaaatctcc
     3001 ttatcaacta tggctagaaa aaacaggaca agtagtactt gaagaatcag gaagtgagcc
     3061 cgcttattgg ggtaatgttt tagaagaagt ggttgctaaa gagtttcagg aacgtacagg
     3121 caaaaaagta cgcagaagaa accaagtatt tgaacatcca ttacatccat ttttaagagc
     3181 aaatattgat cgggaagtag tgggagaaaa tgccattctg gaatgcaaaa cagctaatca
     3241 atttctcggt aaagaatggg aaggcgaaga ggtaccgctt agctatctct gtcaagttca
     3301 acattacatg aacgttctaa acaaagacta ttgttacatc gctgtcttaa tcggtggtca
     3361 aaaatttatc tggaagcgga ttgaacgaga tcaagagctg atcgatacaa tcactgaaca
     3421 attagtagag ttttgggaaa caaacgttct tggaggtatc gagccaatta ttgatggtag
     3481 tcaagcgaca gctgatttct tgaaagagaa gtatgcagat gttgaagacg ttcaaacgac
     3541 gttaccaatt catttcgatg aactagttga acagaaaaac gaactcaaac ggactaagaa
     3601 agaaattgaa tcagctatcc gtcaagtaga caacgagatt atcagcgagc taggtaaacg
     3661 tgaagccagt atcggtatca ctcaaaaaaa catcatcagt tggaaatttg tccgtacgag
     3721 acgtatgaac tcgaagaaac tagcagagaa atatccagat gtcgcaaatg atgaagagat
     3781 ttataacgtt actgaatcaa gaaggctaac cgaaagggag atcaaataat atggcaacaa
     3841 atgaatcgtt aaaaaatcaa ttggcagcaa agccacagaa acaagttgca ccaggtcaat
     3901 taggacttaa agctttgatg aatacaccaa caatgagaaa gaagttcgaa gaggtgcttc
     3961 atgacaatgc taatgctttt atgtcgaatg ttatgactct tgtatctaat gacagttatc
     4021 ttgcagatag tgaaccgatg tctatcatga gtggtgcgtt aactgctgca acattaaatc
     4081 ttgggctaga taagaattta ggttatgcat atttagttcc attcaatagt aaaaacaagc
     4141 aaacaggaaa atgggaaaag aaagctcaat tcatgcttgg ctataaagga tatatccaat
     4201 tagcccaacg atcaggtaaa tacaaagcat taaatgtgat cgaagtttac gaaggagaac
     4261 taaaaagctg gaaccgactg acagaagagt ttgagtttga tccaaatggt agaacgtctg
     4321 atgaagtcat tggatatgtt ggctatttcg agttactgaa tggattcaaa aaaactgtct
     4381 attggaccaa acaagaaatc gaagctcatc ggattgctaa caataaagat cgcgataaga
     4441 caaagttaag tggtgtgtgg gcatctgatt acaatgcaat ggcacgaaaa actgttttga
     4501 gaaatcttct ttctaaatgg ggaatcttgt ccatcgaaat gcaagaagcc accacatcgg
     4561 atgagagagt ccaaagggtt caagaagacg gcagcattat tgctgaaaca gaagttgaag
     4621 aagatattcc tgaaagaaaa gaagcagagg ttatatctga agaaaatgaa gatgtacaaa
     4681 ctggattatt tgatgcatct aatccgccgt taaacaaata atgagggagc tttctccctt
     4741 gcttttctag aggagaaata cgaatgaaca caggatatgt caaattacat cgaaaagtga
     4801 tgaattcatt cgtttggacc aatccttata tgtataaatt atggaacttg tgtttgatga
     4861 aagcgagtca tgagaatcgc aagatacttt ttaatggaaa agagatacaa gtgaacagcg
     4921 gagaattcgt cacagggcgc gatgctatca catctgagat gaacgaaggc gtgaaacgtg
     4981 aacatcaagt gaacagcggt tctgtatgga gatggctaaa acaatttgaa aaaaacggaa
     5041 tgttgaacat caaatcaact acgaaataca gcgttatatc aataaaaaac tggtctttgt
     5101 accaaagcag tgaacaacaa atgaacatca aacgcacaac aagtgaacaa caagtgcaca
     5161 caaacaagaa tgaaaagaat tataagaatg aaaagaataa taaaacatca tcgtcgcaac
     5221 cacgcaaaaa gcgtgtttac gacaccgact cagtttacta cattctcgcg gaggagttat
     5281 tcaaacagat ttgtcagaat caggaaatca aaaagccgaa tctgcaaagt tgggcagata
     5341 acattcggaa aatgatcgag atcgacaagc ggaccgaaaa ccaggtacgg ggaatgattg
     5401 aatggagtca gcacaatgtg ttttgggcat cgaatatttt atctgctaaa aaattgcgag
     5461 aaaaatacga cacaatggca gcgcaagcca atcgtgatta taaaacaaaa caaactaaaa
     5521 cgcttgaata cgagaaattt agcacagatg agttgcctat ttgagaggag gcgtaggcat
     5581 ggaaactgtt ggggaaatca tggaaaagct gatacagaaa gtgcttgtcc aacgtggcga
     5641 atgtcctgaa tgcggacagc ctttgtatgg atggcgtacg aagaaccctg atggctcaga
     5701 acgttgtaag ccaacctgta tgcaatgtgg gtataaagca ttgcgagttc aggaagactt
     5761 acaaacagaa cgaatttaca acgagagcct gaaagcaaga gcaatcaatt ttttcaaagg
     5821 tggttctgtt gtgcctaatc aagcgttgtt tgattgcaca ttgcagaatt atcaaattgt
     5881 cgatcaagaa acaagacaag cggttgaagt aaccaaacgc tttgttaatt cagtcttgtt
     5941 aggaaatcca agtcaccttg ttttaactgg aaaacaagga acaggtaaaa gtcacctagc
     6001 gatggcagcg gcttgggaag tcttgaagcg atcaaactac gataaaaaaa tcttatttat
     6061 cgggttacag gaaatgctgg atcaaatcaa attttcgtac aacaatcctg aactcagaaa
     6121 aacgattgag ggatcgttga ttgcagatat caaaacagcg gatttagtca tcattgatga
     6181 tatcggttca gaactaggaa aagatgcatc agatagtcga gcgtttggca taaacacgct
     6241 aaattcgttc ttggatgcac gacagaacct agcaacgatt atcacaacga acttgcttgg
     6301 tgaagaactg aaaaaagctt acggtacgag aacaatatca agaatgtttg tcaactctga
     6361 tggatttacg atggtatttt ctcaaacagc agacaagcgc ataaaaccag tcaaaggagc
     6421 gatcgcatga atttgactaa acaagaaaaa agaatctgtc gtcagataat gttgcaaatt
     6481 ataggtgatc gtgacatgga cgaattatct caacaagaga gtcgaatggt tcggaagata
     6541 agtaagctaa ttatttatcg ggatacagaa agatttctca ctgttttagg taacgtctac
     6601 gagaatccag agttattgga ggtagagtaa ttgccaaaaa tattagatgc atgttgcggt
     6661 agtagattgt tctggtttga taagaacaat tctgacgtaa catttatgga ttgtcgtcag
     6721 cagtatgaag aattatcaac aggtcatgtg atcaatgttg atccagatgt ggtcgcagac
     6781 tttcgcgata tgccttttga agatgatgtg ttcgatatgg ttgtatttga cccgccacac
     6841 ttaagacatg tcggcgaaaa ctcatggctt gccaaaaaat atggaaaatt agacgaactt
     6901 tggccagaag acatcaggca aggttttgca gagtgtatgc gagttttacg accttcagga
     6961 acattaattt ttaagtggaa cgaggaacag ataccacttt ctgatgtgtt agaggctatt
     7021 ggagaacagc cattgtttgg taataaacga agcaaaacac attggcttgt gtttatgaaa
     7081 aaattcagct aacggaggaa acaaaggagg aaaagtaatg aagattgtat taaataaatg
     7141 ttttggtggc tttgggttga gccatgaagc aaaaatggaa atttttaaaa gaaaaaatat
     7201 tgaagtattc ccatacatca ataactttag ctatgactca gatgatgaat acacgagata
     7261 cacaggccaa aagctaggtt cgatggattt tatctactat ttcaaaaaag acccgaaaat
     7321 cgacaaagtt acaggaattt atagcgaaat tgaccgactg tacggaattg ctgatgattc
     7381 cagtttttca agtgattcaa acagaggtga taaggacctc gttgctgttg tcgaaaaact
     7441 aggaagtgag gccagtggac catatgcaag tcttaaagtc gttgatatac ccgatggggc
     7501 tgagtgggaa ataagtgatt atgacggcgt tgaaactgca cattatggtt ttcaaacagg
     7561 tagtatttag tccactaatg acaactaaag aggaggcaga aggatgagtg aagatgaata
     7621 ctttgaacag tagtctatta acgacagaga tttttaactg gaaaggggtg tagtcatgga
     7681 cgcatcggaa gtagtaaata aaattaatag tctcaaagag gttttcggag acgtcgaagt
     7741 agtgataaac gcacaaggct atgacaacgg attttttaag cagattaccg acattagcat
     7801 tcagcaagga atggaagacg aagatggaaa ctttatcgat gaagtagcca ttttagtgac
     7861 ttgcgagtaa gtaattgaca gctaaagagg aggcagaagg atgaaaaaat tgcaccaact
     7921 catatctgag aaagaaagcg aactccaaaa tttagaagat agtttgggat tggggtttcc
     7981 aattgttgag caagctaaaa tgactcaaat cagtcatcta cgacttgaat tggaagattt
     8041 aagacaaata gaaaaatcaa ttcaactcaa cgacaaccag aaaatcgtgt tggagtggtt
     8101 gaaaagcgag acaattttaa ctagagaagc accaatatta tctgttaatg ctttttctga
     8161 taaaaattta ctaggaaaat tacctgataa agtacgcaaa gcttataaac tattggcttg
     8221 taaacaagaa tatgaagtcc tatcagcatt cgctcaatgg ggattagaac aggaggaagc
     8281 ggaatgaaca acagacatcg cagaataaca aaactaagaa aacaggaact gaatgtacta
     8341 aagacaaagt ttgaaaaaga atatggaatt tcagcagaag aaacatataa agtggcaagt
     8401 cagtttgttg ctgatgcgag taatgctatt cgtaagtttg ggatttcgat attaaatgat
     8461 gatcgtaaat gggaggaaat gagatgaaaa taaaagacgg attttacgct agtagtcatg
     8521 gtatcggcgg tttaatgcta gacatgccga taaagaaccc taaaacacgt cagaaatcaa
     8581 aattcaaaat cggtgacatg gtccgatgta aagcagaaga gttcatctat ccgtttcgtg
     8641 gatatgtaga gcatgtctat aatcattcag caatcattca cattgaaaac acgatggaat
     8701 gcgataagtg gttagcgaaa agcaaagata atttagcagt ggctcgtttg gtggatatgg
     8761 aactaatcaa tgacaaataa aaaagccgga tcgctccgac tgattcaata aatccaacac
     8821 atttattata tcacataaag gagcggtttg acttgatgca attgttacga gaggtagatt
     8881 tcaaacagac aagatgtaat gcgagagatg tgctgaagaa ctttcggcgt ttggagcgga
     8941 tggcaggtcg ctctttgata gatattaagt cgccgattat tacggatatg ccgaaggcac
     9001 cgaagcacgg caataaggca gaagacgcga tcattcagat gatggatata gaagcggaga
     9061 gagacgcaat actagcggct ttgatggctc ttagtctgat tagccgtcag atactctact
     9121 acagcttctg tgacgtaaac aagcactcta attatgaaat agggcaattg atacgaggat
     9181 acggagagaa gaatgtagag aagttgaaat ccatcgcatt aatcgaattt gcagaagcat
     9241 acaaaaaagg cgtgttagtt cagtatcgtt gattttgtag ggtttttgta gggatagtgt
     9301 agggtttttg agcggtttaa cgtgatatta tggtagtgtc gaaagattag tgataggtct
     9361 gagacaaaat aaaatgtaag ggaggaaatc tccctcatcg ttttaactta agcttcgata
     9421 gacagcaacg gaaatattaa gaataaggat gtgaatttca actccttcta aattgttctt
     9481 attatctatc atccgttgtt gtctattaat ttatgcatcg gaggaaaaac gaatggataa
     9541 agaaatcaaa gtaactgtca aattagattt gactgaacta aaagaactgc tcaacaaggc
     9601 tagtgaccaa gtcgaacagt tacaagaaac tttagatgaa attgctaatt ttaaaatcca
     9661 agtttcttag cagtatattt tttagcagct gcactcataa aatcatacca agtatcagaa
     9721 aaatataact taactaaata attaaaaaag gtgggtaaat agaaatgacg gcgttaataa
     9781 tttcaatttt tgcgctctgt cttaatgtct atatgattgg atttaaaaat ggacaaaata
     9841 aaaaatagta gcagccgaga ataattttat agtgtcactg tggcggaaat agaagacgca
     9901 aaggtggagg taggaagcgt atggcgcact atcgggacct tgtacaaaac tagaaaggac
     9961 tagtacgtgt gtggtgcgag ccctagggag gaacaggata accgcctgtg tgtaggttgc
    10021 tattacatat tagatcactc tttgagtggt ctttttattt acataaagga ggctacataa
    10081 tgagaaacta ctggtatata tcgctaacta atgaatatcc tcgaaccatt gatgattgtt
    10141 cagtgcgtgt tgtgcgttct gtccaaatca aaaagaagta ctccattgtt gaaatgacca
    10201 gagaagctac gccaaaagag attgataaat gcaaacttct ttattgcggt catggtttct
    10261 atgatgaacc aaacattcaa aataatatta acaagaattt gagggattag aatgcaagaa
    10321 atggcttaaa aatctccaat caggacaaat caagttagac aaagtttccg atttaaagat
    10381 attgattgaa gctgatttaa tgctaaaaga tattgaaaat tagaaaacaa aactcaacct
    10441 aagaagattg cgaggtggtg tgtattgaat ggcaagacaa cgtgatccaa gacgtgatga
    10501 agccaaaaga atttggttag aatccaacgg agaaaagcag ttaaaggaaa ttgcatctga
    10561 attaaatgtt tcagattctc aggttagaaa atggaaatcg caagacaaat ggagcgctga
    10621 attgaaaagt aacgttacca atggcaaaag taacgttact aatcaaggtg gcgctcctat
    10681 tggtaatcaa aatgctaaag gtaacaaagg aaacagcaga gcatcaccgc caaagagaaa
    10741 taagaatgct cttaaaactg gtgagtatga aacaatattt tttgacacgt taaccgataa
    10801 tgagaaggac atctattcta gtttgaatga tgatccttct tttgctttgt ccgaagaaat
    10861 acgacttctt aaaataagac aacttcgaat gatgaaaaga atccaacaag ctgaagctgg
    10921 actaaatgat gaagaagtcg aacgattgca gcagctaaga aagattaaaa ctccgattga
    10981 aaaaaatggt aaaaagctag aaatcaagcg tgaggttatg caagatgtgc agattagcag
    11041 aaaaaaacat cgcaaaattg atgatattct ttcaattgaa gattcattga ctcggattag
    11101 caaccagtta gctaaagcca tcaagcaaat gaatgaactt tatatgaatg aatacagaac
    11161 tgatttaatt aaagctcaga ctgataagat ccaagctgag acaaatgaaa ttggcggaaa
    11221 taattcaggt gaagaaatag aagaatggaa acaggcagtt ttaaatgccg caaacaaacg
    11281 ggcggtgaaa gaaaatgaat aatgaattta ttccttttgc tgatattggt tctgccattg
    11341 attattacta tgataaacca gtagcttttt gccaagatat tttgcatttg aatcctgatg
    11401 aatggcaaga aaatgtttta aatgatttag ctgaattttc aaaggtttct gttcgttctg
    11461 gtcaaggagt tggaaaaaca gcattagaag caggagcaat actttggttc ttaacgtgtc
    11521 gaccctacgc taaagtaata gcaacagctc cgacaatgaa gcaactttac gatgtacttt
    11581 gggcagaggt agctaaatgg ttaaatgata gcttgatcaa aaacttactg aagtggacaa
    11641 agaccaaaat ttatatggtt ggtgattcag agcgttggtt tgctacggct agaacagcga
    11701 ctaaaccaga aaatatgcaa ggttttcacg aggaccatat gttgattgtg gtagatgaag
    11761 cttctggtgt gtctgatcca attatggaag ctattcttgg tacgctatca ggttttgata
    11821 ataagctgtt gatgtgtgga aaccctaata atattgaagg tgtcttttac gattcccaca
    11881 attcagaccg tgataaatac agagttcata aagtatcaag ctatgatagt aaacgtacaa
    11941 acaaagacaa tatagaaatg attcttaaaa aatatggaaa agaaagtgat gttgctcgtg
    12001 tccgtatttt tggagaattt cccaaaggtg cgttggattc atttatcagt cttgaaacgg
    12061 ttgaattggc tacagaaaaa caaattagtg attctttagt caataaaaca acggttgctc
    12121 atattggtgt tgacgtagct cgatatggtg atgattctac gattctcttt cctagaattg
    12181 ctaccagggc attggagtat gagaagtatt caaaacgtag cactatggaa acaacaggat
    12241 atgtcatcaa catggccaag aatctaatga gtcaatatcc gagtattgat aaagtgatga
    12301 ttaaagtcga tgacactggt gtcggaggtg gtgtaaccga ccgcctagaa gaacttatag
    12361 aagacaagca ttatcctttt gaggtgtttg gagtgaataa cggttcaaca tcagaagacg
    12421 atttttacga taatttaggt actcaactat ggggaaacat caaggaaatg ttagaagaaa
    12481 atatgacagc aaatcttaac ggagaacagc ctgttattga attgccttct gatagttcgt
    12541 taatcaaaga attaagtact cgcaaattca aaatgacaag tagaagtcgt atacgtttag
    12601 aaagtaaaga tgatatgaaa aagcgaaata ttggtagtcc cgatattgct gacgcactgg
    12661 ctttagcgtt ttatgagcca ccaagtcact atcaatttat tcaattttag gaggtgagtt
    12721 tttgattagt acagtattat ctttagagag atataagaag ctacgtgtta aatatgcaac
    12781 acaaattgaa gatggaatgt ttgatccgaa tggttttata gaatatatga aaccattttt
    12841 tgctgatcgc gaaagaaaat atctagctta tactagcgaa aagaatgaaa tagacaatag
    12901 accaaagcct aacactgata ttgtaaaagt taataataaa cttcatgctg gtatgtattc
    12961 aattgtcgta gaccaagcag tcaatcattt tactggtata cctatcaaat gggattacga
    13021 tgtatcggaa caaaaaagaa cactcattca aagattaaaa gataaattct taaaaaatga
    13081 tatagaactt ccaacagttc cagaagcttt taacaaatta acaagcaatc ttgattccat
    13141 gagattcgca atgcttgatt ctgaaactgc aacttttcaa ggagcttgtg gagtagcttt
    13201 ccgattgtta gagcctgtgg aagaagatga tggttggaag ttaagagcaa gtaatattga
    13261 accttggaga gcagaaagat atggaaatgc cggaatctat atcaaagaaa agtatgactc
    13321 ttaccagaaa aaattttttc aagaaatgaa agttatgaca agaaataaaa ttctgacata
    13381 tgcttgctat ggtgatttga attttgttac aagcggaacg ttcaaaaaaa ttgatgagac
    13441 cgataaccct ttggggacga ttaccttggc agaatttaaa aataatacga atcgttattg
    13501 tgattttgaa gtagctgagg aaattggaga tgcaattgat cgtgctttgt ctgatcaaca
    13561 aaacgaaatc gaacaattta agcttgcgta catgcttgtt actggcacaa caatgagcaa
    13621 aggcactgct aaagaaatga tgaatcagct agggattatc aacttaaaag accctactgc
    13681 aaaagctgaa tatgtcacaa aaaaattagc aaaagatttc aatgaatatc atatggatct
    13741 attgaaaaaa cagttttaca ccatttgtaa agcaatcgat tttaacgatg aggtgtttaa
    13801 atcaaacagt tctggagaag ctcgcaagtg gcaaatcatt agcctagaag cgaaaacaaa
    13861 tactaaagag caatatttta gagaaggatt gaaagaatgc gcagaaacaa ttgctgcttt
    13921 tcttaagttt catgacaaag tagaaattga gcctgaaaaa attattttta ctttctctag
    13981 atctttgcca actgatttaa gttatttagc tgaagcttta cctaaacttg caccatacgt
    14041 atccaaacga actattcaga gccaaattcc atttgtaaca gatgttgatt atgaaaatga
    14101 aatgatggaa ttggaaagtg gaagtgctta tccagatagt gaatacaatt ttggcggagg
    14161 tggcaatagt gacgatagaa acgaagtatt ggaccaaacg tcgagaactg gaggatcaag
    14221 caaggctcaa acaagaaaat cagacactta aaaaattaac tagtgtattt cctgaagcac
    14281 tgaaagagat acaagcaaaa ctattatcac aagctgactt acacaatatc acttatccag
    14341 aaatgatgga gttttacagt acaagtaatc agaaaaaata tcgtgaatat gtggaaaaaa
    14401 attataagtc attaaaaatg tatgatgcaa aatacaaaga gtttatcgat gaattttttc
    14461 caccatttga ctatgcaaaa gtcaatcgct tattacaaat acgatcagat gtatttaaaa
    14521 ttcttgcaga atatgcgatg gatgcagatg tgaatcaata tttttctgat cgcttagagg
    14581 aaattcttca aagaacatat tcttctaatg ctaatgtttt tgttcaactt ttaaacgttg
    14641 atataccaaa ttatttacca gaaaatgaaa ttaaacatta tttgaactat ccatggtcag
    14701 gaaagacatt ttcaagaaga ctttggggaa atatttcatc gcttgaacag aaactctcta
    14761 atgctattgt aaaaagtgtt gctagtggag aaggcgttat acacgcatta aacaccatga
    14821 gattagactc agaaatttgt gacatgttta agttagaaga atcaaagtat aacaaagcga
    14881 tagaaaatct cgttcgaacg gagtatgcaa aatttgcaca agatggtatt gaaaaatcat
    14941 atttagaaac aggtattgag gaatacaacg tattgactgc aaaagatgag agagtttgcc
    15001 ggatttgtgg aggaaaggca agtaagaatc cctataaact gaaagatgct gtcataggtg
    15061 aaaatcgagc acctttccat agtcgttgta gatgtacgga tgttcctaat ttaccaaaat
    15121 taggaaagga tattgatgaa gaatatgacc gtttatttgg cgatttatta gatgagtttg
    15181 cacatgattc ttttggaatt aatttgaaac ggaggaagta gaatgaaaga tttttttgaa
    15241 gcagtactaa caattaatgt aaatgctgat attgcagaag cctacaaaac agctattgag
    15301 tctgagaacc atcctaatgg cttgagagac cattggaatg gcaattatgc ctacgtggtt
    15361 attggcgatc aaactgttaa ttatcaagat aatactccag ttgataagaa taccgttaat
    15421 ttaacgattc aattattatc tcattcatta ccaaatttaa aagaaacagt tgattggtat
    15481 gaaaagatgg gatgtattgt tgttagaact gactacaaag aaggaaagtc tagtaattag
    15541 gcttttttat tttgtccgaa atgacgttaa actagcgcaa tactgggctt ggttgaatgg
    15601 tggggcgcaa ctattaaaac tcaaagcaat gcggggcgtg aaaacgaatc gtggggcgaa
    15661 aggagaatga gcatgaaaca caaatcatta atgccaatga atttgcaata ctttgccgaa
    15721 ggtgacgatc aaaagttttc ttttgatgac ttcaaatctt ttgtggaatc caatgaagaa
    15781 gctcaaaaat ttattcagtc acaatctcaa agtgttgccg acaaacaatt ggaagcttgg
    15841 aaacaaaata acttagataa aattaaacag gataccatca aggaatatga ggaatctaag
    15901 aaaaacaagt cacctgagca aattcaactg gaaaaactac aagctgaatt tgaagcagaa
    15961 aaagcgttgc gtgtgacaag tgataataaa gcatttgttg cagaacaaat tgctggatta
    16021 gaactagatg gagagttaaa agagtctatt tctcaattta tgctaaataa tcttgttagt
    16081 tcggatacag atttcactaa gaatgctgtt gaaggtttta caagtgtttt gaatgcaatt
    16141 aatgagaaac atgcagatgc attaaaagaa ctacaaatga agtctgcatt tggtggaact
    16201 caacaatcga ataaccaggt tcagcagaac aatgaaacat taacaaatcc agaagaacaa
    16261 ttaggacaaa ttcttcaaca atttaactag gagagtgaaa aattatgaaa aaaacatctt
    16321 taaataattt agagtatttg gatatttcac cagcgattaa tgctatgcaa gtaccaaata
    16381 cacctttttt aagctattta cttggtgctg gaaaaacaga gccagcaaac tcgacagaaa
    16441 ttaaatggcg tgaatatgat atcaacaacg atgattcttc tgaaaaactt gagggcggag
    16501 aatatccaga tgctgaatca ggtcgaactt ggtttaacaa ctatactgaa atttttagaa
    16561 aatcaacctc tgtatctggt acattagatg ctattaatgt gaatggagtc ggaaatgaat
    16621 taactaatca agtagcacta cgtggtatgg aaatgaaaat tgatttgaac cgaaaattga
    16681 ttactggtgt aaaagctgat gaaaatggtt ctaaaggtcg tcgaatgaat gggattttga
    16741 acttgatcaa ttcagcaaat aaggcagaaa cagccactgc gggtgcagta acaagaaaag
    16801 atatcgatac tttatttaaa ttgatgtatg aaaaaggtta tatgggagaa aaactatgct
    16861 tgatttctcc agatatgcag gagttaatga ctgatgagtt agatggaaaa tcaacaaaaa
    16921 ttgttcagtt cggggaaaga gtaacttttg gattgcaaat tggaaatatc gtgtctaatt
    16981 acggtacagg tattgctcta ctagaaccat cattgccaaa aggaacaatt gccgcaatcg
    17041 atactaatta tgtgaaacta cgtccattac gtgaatggag agcagaagaa cttgcaaaaa
    17101 caacggattc tagacgtatc ggtcttgtag gtgaatactc tcttgaatac aatgcttcaa
    17161 actctggggc aattttaaat ttaaagtctg aataaaaggg agttagtact cccttttttg
    17221 ataggaggaa ttacagtggt aaaaaaagat gaaactaaaa aagatgaagt cgtgaaatat
    17281 agagtaggta aaactaaaaa ttttgttggg tttgttcatc ctaaaactcg tagatttatc
    17341 acagcagatt caaataacga atttatcatt tctatagatg ataaagaagc aattgcaatt
    17401 ttggaagatg caattgatgt taatgaaatt taggaagtga tctgatggat gaatcgctaa
    17461 aaacggaaat cattgagtct acaaaagaag attttccaga tttgagtgaa gaacgcataa
    17521 ctaatttatt agaaataatt ttgctagaaa ttgaatcata caacacttgt aaaaatgata
    17581 tttcatggga aaagttaaaa agcgtgatta gtgaagtgtt gtataaaata ataaaaaatg
    17641 aatcagaaaa aacagtatct tcagttagac gtggcgatac aacgattagt tatgcttcaa
    17701 caacaaatga tgttagtgag ttacttctag gctatggtga tttgatacga agagttattg
    17761 gttgtggagg attggagttt ttttaatgaa tgaagcagat attttagaaa ctacttatga
    17821 agatagttgt attattgaaa gactgacgga cattgaagat tctaatacaa atattactat
    17881 tcaagattac aaaaaagtat atgataatcc tatttcttgc gctctttcac aaggtcaaat
    17941 cgatggacta gcagtcatag aagatggaga gatggtaaat gtttcaactg acacatataa
    18001 attatttgtt catcctaaga ttaaactaaa aaaaggagat cgaataacaa taactcaaaa
    18061 ggccagtggc ttaatttttt ctctatttgc tactaagcct ttttactatc ctagtcattg
    18121 tgaagtgaat ttgataggaa gtgagaaaaa tgggtaatct caaatatgaa tcaaatgcag
    18181 aaaagatcat tgagaatttt aaaaatatga ctgtaattgc tcaaaaagaa ggaatatcct
    18241 tcgttaacga ttcaatgaat aaagtcgtta gtttagctaa accattaact cctgtaaaat
    18301 caggtaattt aaggcgagga tatcgtgtag taaaagctag aaaactatca agcggtcgaa
    18361 ttgttggagc agttataaac aatgaacatt attttaaata tgtagaagaa ggtcatagga
    18421 ctaaaaatgg tggctttgtc aaagggaaat tcatgttaac tcgtgcaacg aatcttgcaa
    18481 atatgtctta tattcctcga agatttaaac aaatggcaat aaaaatcgtt aagaaaggaa
    18541 agtaacatga aagatgaaat cattgctgca atcagtagca aattaaaaga aatctatcct
    18601 gatgggacaa tatatcttga ttcagttatg cagtcaacta aagattttta tttcgtttta
    18661 tcagtaatgg aatctggaac tgaaaatgta ggaattgata ttcaaaatgt ttctttctta
    18721 attgatattg cattgattga taataaacct aatagaaatt taattaatga attagtctca
    18781 cgttgtggga ctttttttaa tacgattaca atcgatgagc aaatattatt tccaaaagcc
    18841 tatttacctg atgaagcaga tggtgttcaa catattcgtt ttacattaga atttccacaa
    18901 tatattgaat ggagtgaaag ataaatggga gaaaaaagaa gtaaaaccgg aattatttct
    18961 gtcgaaaaac ctacttggtt tcctttggaa gatgagacag ggaattttcc ggtatatggt
    19021 actgcgttgc caatgggaac agcagtaagt atcaaaccaa cagctaatta tgaaacaaca
    19081 caagattatg gggattcagt ggttcaagat caatttacag cttttggtgg tgcagaagtt
    19141 gaactggaag caaatggata tactcataaa gttttatcta caattactgg agggaaaatt
    19201 gttaaaggtg gtgccttgcg ttctggagaa gatattgcgc aagatggtgc gtttgcatat
    19261 agacgtaaaa aatcgaatgg gaaataccgt tatacggtct tttataaagg acagtttgct
    19321 ctagattcag atgaaacatc aacgattgaa ggaagtaaag taagttttac tcatccaact
    19381 tggaagggtt catttgtaga tgtaccagga cttggatata tgtattcagt tgatgaagat
    19441 gatgaaggcg tggataaagc aatgatcgga aattggttta caaaagtagc aattccaatt
    19501 gaagaagcag aactttcagg aggtacagaa taatgtcaaa atatcaaaca acgattaaat
    19561 taacgaaaaa agatgaagaa ggtaaatatg agcaagtaca atttaaatct gctgaatttc
    19621 taccaggccc agtcgtagaa gaagcagcag aagtaatgga gggaatgcaa aatgctgtag
    19681 atggaaaatc agtatcagaa gcattaagtc gagcttattc atttattgca gatattttgt
    19741 ttgaaggaca gtttactggt gaagattatc gtcaagggat cgatgctcga gaaattgctt
    19801 cgttgacagg taagttatta aaatctgtta ctgcaggctt tgatgaaact tatacagaaa
    19861 cgaaaaaaaa gtaagcgaag ctctcaaata tccttttttt aaacattcga ttacttatcg
    19921 agaattagat ttaaagacgc aattactcga agcaggttgg acgttaccag aaattgaacg
    19981 tactgacttt gacgagttaa tgcgtctttt tgcttttcga gatgcagtaa aagagcatga
    20041 ggatgttgag tactacgata acttcaccca attttaggag gtgatacttt gaataacgat
    20101 gacctaattc tgaagatgat actagatgaa tctggtttta ctgccggtat gaataatgct
    20161 gtcaaaaagt tgaactcttt tgatgaaaca atcgaaagaa aaagtagaaa tagtggcagt
    20221 tcattaggta gtatttggaa aatatttgct ggaagttttc tagctagcgg agtaactaga
    20281 atcgtaggag ctggctttga tctaatcaag ggttccataa gtggagcggt tgatcgagta
    20341 gatacgatga ataacgctct acgaaatttc caaaatatgg gatttagtaa ttcagaaatt
    20401 atgaagaata tcggaaagaa tgggctttta tctcaaggta ttcaaggact tcctactgct
    20461 ttgaatgatg cgataagtca tgttcaactt cttgcttctt ctacaggtga tttaactcgt
    20521 tcaactcaaa tatttaaagc tttaaatgat ggaattcttg gtttcggtgg ttcaactgat
    20581 caagttaatg aagctgttat tcaattatct caaagtttct caaacggaaa agtagacgca
    20641 caaacttgga actcaatgat caacgctcaa cttggaccta ctctttctgc tattgctaaa
    20701 aagatgggaa ttacaatggg agatctgaaa gaaggtttat ctcaaggtaa gatttctgtt
    20761 gaagaattcc aaaatcaatt aatagaaatg gataccaaag gtggcggagg acttaaatca
    20821 ttaagtcaaa tcgctaaaga ttctactaag ggaattaaaa cctctataca aaatgcaaaa
    20881 acagctgtta cacgtggtgt tggtgaggta atagaaggat taaataaagc attagtagat
    20941 tcagatttag gtggatttaa gggaattatt gataaagtgg gcagttcaat ggaatcgttc
    21001 ttaaaagtaa ttgctgcaaa tattcctaca gcagtatcat ttttaagtaa tttatttaac
    21061 gaaattcaaa aatttggttc tgctttgaaa ttcatgatgc cctttcttgt tcctgcagct
    21121 accgcttttg gagcgttcat gtttcaactt aaaggtatac cagcaataat aaaaagcttc
    21181 aataatttta agaatgccat aattggcgtt ggaaattcat taaagattat gggggcaata
    21241 gctgctgcta attcatttgt tttaattatt ggtgcagtcg ttggtgcaat agctgttttc
    21301 ggttacttta tggcaaccaa tgaagagctt agaaacaagg ttatatctat ttggaatgat
    21361 gtgaaagatt ccgtaattgg tgtattaaaa aatataaagg actggggaat tgatacttgg
    21421 aattctgcta aagaaatggc atcaaatgca gttgagggtg tcaaagatgc ttggtcagga
    21481 acaaaggaat ggttttctaa cacctggaaa ggtattaagg atggggccac aggtttattt
    21541 gataaaacag tagagacttc aagaaatgca gtcgatagtg taaaaaatgc atggtctaat
    21601 atgaagaaat ggttttctga tacttggcaa agtataaaag attcagccag agaaaaatgg
    21661 aatggaatca aaggttctat tatgtcagtt gctggtccat tgattacagg tataaagaat
    21721 gcatttttac atgttacttt ttatctagag accttatgga ataatttagt tgagattggt
    21781 aaaaacgtct ttgagatttt aaaaaatgtt atacttgcgc cggttttgtt tattacctcc
    21841 atgatttctg gtggatggga agaaacaaaa aataatatga ttgctgtttg gaataatatt
    21901 aaagaaagtg ccataaatat ttgggaatct ataaagaata tatttgtaag ttattttaca
    21961 aatatttatt ttgctgcact caatatttgg acagggttca agcttacatt gataaatatt
    22021 tggaatgaag tggtaaatca agctaaatcg atttggttaa acataaagta tttttttatt
    22081 aatctttgga ttgatataaa atattttgca attcagaaat ggattgaatt gaagttttct
    22141 attattcaaa cttggatcga tttaaaatat aatgccatta ctacttggaa taatattaaa
    22201 cagtttttca aagatacttg gaaaaatatt aaagatacgg catacaatac gtggatttct
    22261 ataaaaaata ccatgattaa tacatggaat aacattaagg actctttctg gaatattgtt
    22321 actggaattg ttaattctgc tgaaaatgca tggacaaatc taaaaaatgg tgtttcaaat
    22381 gctattaatc gggtaaaaga aatctttaat tcgttaaggg aagtcaattt atttgaaatt
    22441 ggcaagaaca tcattgatgg acttataaat ggtgtaaaag aaaagtggaa tgctttgaaa
    22501 gatactatca aaggtattgc aggtaatatc aaaaattgga ttaaaggagc tttagatatt
    22561 cattctccat caagatggat gcgtgatatg gttggtaaaa atattgttca aggtattatc
    22621 gttggtattg ataaagagca aagtaagtta gatcaaacca tgacaaattt agtaaaaaca
    22681 ccgtctgttc aacctgtagt aaatggaata agtcacctac caattacaca aacaaagcaa
    22741 aatatatctg ctagtgaaac aaaagaaatt catttacatt taaatgttta tggagattta
    22801 cctgattcaa tggttcgaaa gattactaca aaaattaaaa cagaattgac aagacaaatg
    22861 aaacgagatg ctggtgcagt aggaggaaca ttatatgcaa cttaagagag gacaattttt
    22921 tattaatcaa cattattctt ctgaatttaa tgtgtatatt caaaacaggc ctgcttctgt
    22981 ttcagctagt cgagtaattg aattgagaga gcgagaagga aatgattcta tcattattga
    23041 taaaggctat tacaaaaatg tgactagaaa gattgagtgt tactataaag caccttcaat
    23101 tgatatggtg caggagtggg aagatcgaat cactgaatgg ttagatatgt cgtcttatag
    23161 tgattttatt ctttactatg atgaacaata tatttatcaa gctgtagtaa tagaaggacc
    23221 tgaatttaaa ggaacaagaa aaacaggaaa tattgtccct tttgaattta cagtgagtat
    23281 tcgaccattt aaggaaaatt acaatggtag atttactatt cgacaaacag aaacttttga
    23341 aatttataac ccagagaagt attcttcaaa accgcttatt aaattgagtg gttctggaga
    23401 tgcttctttt tatattaata aggataaata tgatttgaaa tcgttggaca gagaattatt
    23461 tatagattct aaattagaag aggcttatcg aaaattagat ggtaatttag aacatcagga
    23521 tcaggtcacc ttatttttag attttccatt tttatatcct ggaaaaaatg aaattaaatg
    23581 gacgaaaaat attcattcat ttgaaataat gcctaggtgg tggagaaaag tatgaaacca
    23641 agaatatata gtcctactga aacagatttc tcaacgaatg gcttaggaat tttaaaggat
    23701 tgtacaagat gtgaaatata tgaagtagct aatggaaaat atgaattaga gttggattat
    23761 cctttaggaa ctagatttga tgaatatttt gaaaatgact atcaaataaa agcaaagcca
    23821 aacgatcaag aagagtatca tatctttttt attgatgata aagatataga tactttttta
    23881 aatacagtaa ctatttatgc tcagagtcgt acaaatcgac ttggaagacg ggtagtcact
    23941 cttgtggaag tggactctaa aactggtcaa gaagcgatat caattattga aactaaaatg
    24001 gataaaaaat ctgacatacg actttattct gatattacgg ctgtttctag cacaatcttt
    24061 gaagcgagaa atgttttaaa ttgtattgct ggtgaacaag gatcattgct tcaatattgg
    24121 ggcggagaaa ttaaacgtga accatttaag ctttctttgt taaagcgaag aggtcgcaat
    24181 aatgttggaa cgattcggta tggaaaagat atgtctggtt tgaaggtcaa attagattgg
    24241 acaggtataa aaacaagaat tattccatat gctgatcctc aaagtgaagt aggtacgact
    24301 aatcgaattt atggttcgcc agtagatagt gcctatatta ataactatcc tgatgtctat
    24361 acagaacatg ttcagtttac agaagaacaa ggagtaaaag acattaagag cttaaataaa
    24421 atagctaaaa attactttaa aacgatcaat cctggctgtg ataagcctaa agtttctatt
    24481 acggttgaat ttgataagtt gactgatagt gaagaagcga aagaatttgc gaagattaga
    24541 aattatggtc tgtttgatac atttaaaata tatcataaaa aatataatat ttatcttgaa
    24601 tcaaaggtta gtggactaca atacgattct ttatctgaaa aaactttgaa attagaagca
    24661 ggagacattc aagttgcttt ttatcagcaa caagctgtaa ctattcagga taaattaaaa
    24721 gattatgcaa cgaataatta tatgagtgat tttaatgatt atgtttcttc aatgattaca
    24781 ggtcaaggca gtgcaggagg atatgtagtt ttatggccaa aagaaaaacc ttccaacatc
    24841 tttattatgg ataatccaga tttagaaaag gcaagagaag tactaagaat gaataaaaat
    24901 gggattgctt tttctaagaa aggatggcgg gggcctttta attcagcttg gacattagat
    24961 agtatattta atgccaattt tatacaaact ggattaatca aagcagatat ctttcaaaat
    25021 tcttttaaca agactggaga tgtattgaaa ttagttaatg gactactcca aatttggaac
    25081 aacaaaaaga aaataatgga gttaactaaa aaaggaatgg aattttggaa ttctaagggt
    25141 tcaattggaa cgattggaac aactgattct gttggtaatc cttttcctgg ggcttctact
    25201 ccaaccccta ttgaagataa ttctttagtt attcgtacaa atggagacgg caaatatatt
    25261 ttgatttctc ctaaagtagg aaaaggatta gttttattag gaaatggtaa agcaatttat
    25321 tttggagact tagatgtaca aggaaaactt acagttaaag gaaaagaaat aattcctgga
    25381 caaggtggtg gtccatcggg aggtggagaa tctactggtg ggtatcctag tgaattaaaa
    25441 acagatgcag aaaagagagc ttggagaatt tacgatattt tgtgtaataa cggatttacg
    25501 aaacaatctg catgtggaat attggggaac attcaacaag aaacaggagg aacttttgat
    25561 cctgatactg ttcaaatagg tggaccagca tacggattag ttcagtggga tggttcttca
    25621 tatcctcttg ttggtccagc aacatgggat ggaaaagttt atgttcaaaa cttatttaat
    25681 gctgcaggta ttaaagaacc aataacgagc ttagatgcac aagttcgatt gcttatttgg
    25741 acatttacaa atggacaatg gatgg
//
LOCUS       DACCYH010000034        25737 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.034, whole genome shotgun sequence.
ACCESSION   DACCYH010000034 DACCYH010000000
VERSION     DACCYH010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.034"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            624..1952
                     /locus_tag="IVY85_001366"
     CDS             624..1952
                     /locus_tag="IVY85_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8044391.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2154..2987
                     /locus_tag="IVY85_001367"
     CDS             2154..2987
                     /locus_tag="IVY85_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP8044392.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3003..3740
                     /locus_tag="IVY85_001368"
     CDS             3003..3740
                     /locus_tag="IVY85_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP8044393.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3895..4863)
                     /locus_tag="IVY85_001369"
     CDS             complement(3895..4863)
                     /locus_tag="IVY85_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP8044394.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5095..5928
                     /locus_tag="IVY85_001370"
     CDS             5095..5928
                     /locus_tag="IVY85_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP8044395.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5962..6801
                     /locus_tag="IVY85_001371"
     CDS             5962..6801
                     /locus_tag="IVY85_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8044396.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6827..7387
                     /locus_tag="IVY85_001372"
     CDS             6827..7387
                     /locus_tag="IVY85_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8044397.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7586..9307
                     /locus_tag="IVY85_001373"
     CDS             7586..9307
                     /locus_tag="IVY85_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP8044398.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9472..10614
                     /locus_tag="IVY85_001374"
     CDS             9472..10614
                     /locus_tag="IVY85_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8044399.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10630..11841
                     /gene="thiI"
                     /locus_tag="IVY85_001375"
     CDS             10630..11841
                     /gene="thiI"
                     /locus_tag="IVY85_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP8044400.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12414..13061
                     /locus_tag="IVY85_001376"
     CDS             12414..13061
                     /locus_tag="IVY85_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8044401.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKLLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            13454..16099
                     /locus_tag="IVY85_001377"
     CDS             13454..16099
                     /locus_tag="IVY85_001377"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP8044402.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16409..17722
                     /locus_tag="IVY85_001378"
     CDS             16409..17722
                     /locus_tag="IVY85_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP8044403.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17712..18365
                     /locus_tag="IVY85_001379"
     CDS             17712..18365
                     /locus_tag="IVY85_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8044404.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18420..19112
                     /locus_tag="IVY85_001380"
     CDS             18420..19112
                     /locus_tag="IVY85_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP8044405.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            19262..19462
                     /locus_tag="IVY85_001381"
     CDS             19262..19462
                     /locus_tag="IVY85_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8044406.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(19716..21395)
                     /locus_tag="IVY85_001382"
     CDS             complement(19716..21395)
                     /locus_tag="IVY85_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8044407.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(21397..21609)
                     /locus_tag="IVY85_001383"
     CDS             complement(21397..21609)
                     /locus_tag="IVY85_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP8044408.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            22002..23732
                     /gene="efrA"
                     /locus_tag="IVY85_001384"
     CDS             22002..23732
                     /gene="efrA"
                     /locus_tag="IVY85_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP8044409.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            23729..25489
                     /gene="efrB"
                     /locus_tag="IVY85_001385"
     CDS             23729..25489
                     /gene="efrB"
                     /locus_tag="IVY85_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP8044410.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
BASE COUNT         8436 a         4409 c         5447 g         7445 t
ORIGIN      
        1 tcgtaggttc gagtcctact gccggagtaa ataaagctta tgcaaagaaa aagaaatcca
       61 atcacttggg tttctttttc ttttattatt atgatgaatc aagctttctg tttttgtcac
      121 gtatgttgta atctttacga aagagtagag ttggctactc ataatcaacc atacttccca
      181 tcatcgctaa atacgaaata aatcagcatt cctacaccaa tcaaagcaca agctgcacca
      241 agaaaaggga tgacattccc aaagttatct gtattcataa gtatgccacc acccacaaac
      301 aaaaagccgc cagcaatcag gtttttcatt ttattcatct cctttcttac gttatagtat
      361 ttccttcttt caatgagtat tttgaaagct atcttattgt atgatgttct tctaggcctc
      421 ttctttatta tacaggccat tttcgttatc tcatagaaat attccttttt atctgaaaac
      481 tattgtatac cgtatgacat ttttcttcat gaagttgtaa gtatattctc attcaaatga
      541 aaatttcatc atagaagaga ttgtatttct aaattcttaa gagtaaaata cgaagtataa
      601 cttggatttg gaaggatgat tggatgaaag ctattacgcc tgatatgaca acaaaattta
      661 acaaacagtt tcaagcaaat atcaaacagc aggcattgca gcgggcagtt gtgaagaacg
      721 gaattactgc ttctgctgaa aatgtttcag cacatgtgga gaatacaccg gtattttcta
      781 tagatttggc tacggggaaa gtggctaatc aaatgcaatc ggggcgttgc tggatgtttg
      841 ctgcactgaa cacattccgt cataagatgc tgaatcaatt ccatttaaaa gagtttgaat
      901 tatcacaaaa ttacactttt ttctgggata aatatgaaaa ggcaaattat ttctatgaaa
      961 acatcattgc aacaggaaaa gaggcaatcg atagccggaa agtagctttc ttactcgcta
     1021 ctcctcaaca agatggcggc caatgggata tgatcgtttc attattccaa aaatatggcg
     1081 ttgtgcctaa atcggtgatg ccggaaagtg cgaatagttc aaattctcga gatttgaata
     1141 actacttgaa caaattactg cgtaaagatg cagtagtgtt acggaaaatg gttgctcaag
     1201 gagaaacatt agacgctatt gaagaaaaga aagaagaaat gctagaaagc atttataact
     1261 tcttagctat ctcattagga acacctccaa aagaattcga ttttgagtac cgcgatgaag
     1321 aaaagaacta tcatcttgac cgaggactga caccacaaat tttttatgat aaatacatcg
     1381 gtgtaaaatt agatgattat gttagcgtga tcaatgcacc aacaaaagac aaaccattca
     1441 atcgttctta tacagtagag atgctaggaa atgtcgttgg cgggaaagaa gtcaaatatc
     1501 tgaatgttga tatggaaact ttcaaaaaat tagcgattgc tcagcttgaa gaaggagaat
     1561 ctgtttggtt tggttgtgat gtaggtcaat cttctactag aactagtggc atcatggcct
     1621 tagatgctta ttcaatggat gatctatttg atacggattt tacaatgact aaagctgaac
     1681 gtttagattt tggagaaagt ctgatgaccc atgcgatggt attgaccggt gttgacttag
     1741 tagacgggca atcaacaaaa tggaaagtcg aaaatagctg gggcgaaaaa gttggaaaga
     1801 atggcttctt tgttatgagt gatgcctgga tggacgaata tacgtaccaa atcgttgtaa
     1861 gaaaagattt attgacgaaa gagcaattaa atgcgttcaa tgaagaacca attgttctat
     1921 ctccgtggga tccaatgggt gctttagcat agacgagtat ttcaatgaag agtatgtagt
     1981 aaagataaaa agagctggct taagccagct ctttttatct ttaaagtcca ttccctgttt
     2041 tttatggtcc ctgtgctata attctaaata aaggataagg attggaaata atccttttat
     2101 cattaaaaat atgatgacga agtagaataa aattgggagt aggggggaaa gtcatggata
     2161 tccaaactgt tttatctgat ctgaaattaa atggtaaagt gattccagtt gttggtggcg
     2221 atgttaatca gacctatcgt ataaaaaccg aacatcgtgc ttatttttta aaaatccatc
     2281 cgaatgtaaa aaaaggattt tttgaagcag aagtagacgg attaaaagaa ctgtcagcat
     2341 tcgtacgtgt accggatacg tatatgctag gtgaaacaag tgaaggcgct tatttgttaa
     2401 tggaatggat cgagccggga aaaggggatc aacgagatct tgctgcagct ttagctaatc
     2461 tccatcagca aacagcgcca caatttggtt ttcgtaaaga taactatttg ggtacacttg
     2521 ttcagaaaaa ttcttttgaa gaagattggt ggactttttt ctttaaagat cggcttgaat
     2581 cccagatttc tttggcagaa gaaacgaatc gatggaatgt acagcgtcaa gaaaagtatc
     2641 tgcgatttaa agaacgtgta ttgaaatcgg ttgaaccaaa aaagatcact ccacgcctct
     2701 tgcacggaga cctgtggagt ggtaatgtgt tctttgacca gcaagggcat ccagtttttg
     2761 tcgacccagc tgtttcttat gggaatcggg aacaagatat tgcaatgagc cagttattcg
     2821 gtggtttccg accggaattt ttagatgctt atcaaactat atttccatta gaaaagggat
     2881 ggaaagaccg actgccgatt taccagttat attatctttt ggcacatttg aatatgtttg
     2941 gagaaagtta cggttcccaa gtagatcaat tactagaaaa tttttaagag gagggcgtgt
     3001 ttttggcaaa aaaatatacc aaaaagcatg aagtttcata ttatgagtgt gacatcaacc
     3061 aaacgatgac atttccttcg atgttaggag tagttatcaa aacgtctgaa gatcaaagcg
     3121 accagttggg aagaggcagt gaattcgtca atagctttgg acttacttgg gtcatcacga
     3181 attattccat ggagattaat cgtctcccta gagtcggtga agtaatctca gtaacgactc
     3241 aagccatgga gtacaataag tttttctgtt atcggaactt ctggatccat gatgaagatg
     3301 ggaaagaatt agtcaaaata gaatctgttt tcgtattgat ggattttgtg aatcgaaaaa
     3361 tgagcagtgt taatgaagag atcattgctc cttttgagag tgagaagatc aagaaaatca
     3421 agcgtcagga aaaaattgaa aagattgatt cgggcttcat gttgccatat cgtgttcgtt
     3481 tttatgatat cgatagtaat cagcatgtta ataatgcgat gtattttaat tggatcattg
     3541 acgtattagg atatgacttt ttgactacgc atgttccaga aaaagtaatt attcgatttg
     3601 ataaagaagt agagtacggg aatgagatcg aaagccattt tgaacaaatc cctatcgaaa
     3661 atggtgtcaa aacaaaacat gaaatccgaa tgcaagaaca gctgtattgt gaagcaaata
     3721 ttatatggaa aaataaataa aaaagaggtt gtgacagaag ggggcaactt caagaaataa
     3781 gaagaaattt acgaaaattg tttctcaaat ttttgtaaat tttcgactta gttttcgaag
     3841 aagctatttt tgtcacagcc tctcttttta agtttagcta ttagataatg atgtttagtt
     3901 ttggataaac gaaatcaatt cttcacccga caaaccatag tttacagcaa tcaatagttt
     3961 taaacgtgct ttttgactat taatactgtt acagaaaata atccccattt tttctagttc
     4021 ttttcccccg ccttcataat cataaacagg ctctgcgatt ccattgaaac aacgagaaac
     4081 aagtactaca gggatttttt ggacaagaag cttttccaaa gcagctaagg cttgtggagg
     4141 gagattcccc gcacctaaag cttcgattac caaaccgtct actttcgtat gggcgatggc
     4201 ttccaataag tccccttgca tcccagcata tgctttaacg atcgggatag tcccatctac
     4261 agcggaaatg tccagtcgct ttgtttctaa aagttcctgc aagaataaaa ttcgattctt
     4321 agttactaag ccgatgggac ccaatgtagg tgttcggaaa gtagcaacat ttgtggtatg
     4381 ggtctttgtc acgtagcgtg cagaatggat ctcatcgttc atgaccacca aaaccccctt
     4441 atctagcgct tcttcacaac ttgcgacacg aatcgcactt tcaaaattat aaaggccgtc
     4501 acttccaagt tcattacttg aacgcattgc cccagtcaaa acaattggta atttgttgcc
     4561 aatcgttgta tcaagaaaaa aagcagtttc ttctaatgta tctgtaccgt gtgtgatcac
     4621 cactccggat atacctgatg cataagcgga caagatacgt tcttttaata ccaacatgtg
     4681 agaaggcatg atgtgtggtg agggaagatt gaagatatct tcaactaata attctacatg
     4741 cttggggatt gtgatttctg catctaataa tgggtttgcc acatcaggac taacaccgcc
     4801 cgtgagcatg ttttctttca tggcgatcgt tccgccggtg tgtagtacaa gtattttttt
     4861 cattttacag acttccttac gaataatttt gcatagccgt taaatcttat tgtatactat
     4921 ataaaagagt tttgctatca gtgaagaata acgaatagcc taacttatca taaaaaagct
     4981 gaccttatta aaatggaaaa gactcaacct tgacctccta atacaatgga gtatctggtt
     5041 gagtttcttt tacaagaaag cagccaagga aatcaatcac gaaggtggcc ggaaatgatc
     5101 agaatcggat taacatcgtt taaagaacat gacaaattaa ctggtaaaaa gaataacact
     5161 ctttatgaat atgcagggtt tctaccctta gtggaattag atactgccta ttatggaatt
     5221 ccccgaaagt cctcagttga gaattggata agtgaagtac ctgaatcatt tcgctttgta
     5281 gtcaaagtct atcaaggaat cagcggtcaa ggagactgga agccttatta tcagaccgaa
     5341 gaagagatga ttgcgcattt tttagatgtt atgtcgccaa tggtagatgc tggaaagatg
     5401 ttttgctttt tagtccaatt cccctcatca ttcaaatgta cgaaagaaaa tgtccagtac
     5461 ttgcgcaaac ttagaaagtg gtttgagggt tatcctgttt ctatagaatt gcgggattat
     5521 tcttggtaca gcgatgaatt tcttgaacgt acaagaaact ttatgtctag agccgctttt
     5581 tctttagcca tcatcgatga gccacagttg ctgaatacaa cgattccttt tgatccattt
     5641 gtcaccaatc cgaactttgc tctttttcga ttccatggta ggaatgcgca aggatggcaa
     5701 gcaaatgata aagagtggcg taaaaaaaga acgttatatc gttattcaga aaaagaactg
     5761 caagaactag cacatgaagt caaagtagtt gattctaaga caaaagaggt aggtgtgatt
     5821 tttaataata attctggtgg tgatgcggct gaaaatgcat tagcactgca aaaaatatta
     5881 aatatcaagc cagatggact caatccaacc caaatggatc tgttttaagg gcttatttta
     5941 ttcaagaaag gggcgaagtc tatgatcaaa gctatttttt ttgatattga cggaacattg
     6001 gtcaccaatc gttcaaaagc gttggaatca acaaaacaag caattgaata tgctcggaaa
     6061 aacggcattt tatgcggtgt agcaacgggg cgcagcccag taaagatcaa agaaatcatt
     6121 gatgaattgg aattagatat gtatgtcgtg tataatggtc aactggtttt tactgcagat
     6181 cgcacgatta tcgaccaccc ttttgaacaa aaagtattgg aacatattgt tgaatttgca
     6241 gatgagaacc atcgacaaat cgtttttggt gcgagaaatc ggttagatgg atcaacgacg
     6301 atgctattag gacaatcgat ttttatcaag cgactagtca gctttttacc gcgtaaattc
     6361 ccggtacgtc tgatgaaaaa aatcctccag gtttttagcc ctcaccgaca aaaggatcgt
     6421 tatgaaacgt tagagatatt aaaagaacca atttatcaat gtatattgct cagtccggag
     6481 tcagaacaga aaaagttaga acatcgcttt cccgagtgta cgtttcagcg ttcaaatacg
     6541 tatactgtag atatcatccc aaaaggtggt tcaaaattac taggcatcca agctttcgca
     6601 aatgctgtcg aaattgaaat ggaagaaatt atggcattcg gcgatcatta caatgatatc
     6661 gagatgctga aaggtgtagg aattggagtg gccatgggga atgctcagat cgaagtaaaa
     6721 catgctgcag actttgtgac tcaatcaaat gaagaagatg ggatatacga aggactgaaa
     6781 cacttcggtc tgatttatta gtcaatgaaa gaaggaaaaa atcagtatgg aagcagagac
     6841 accttttcaa aaagtagaaa cattccacaa aacttttgat ccaagaagac cagagatacc
     6901 cacggctttt acagaaaatg aagctcatac acgtgctgcg ttcaaaatag aagaacttgt
     6961 tgaatttgtt gcagctactt cacctaataa agaacaactg catgaagctg ttatgctgct
     7021 gcacgaagca ttagataaag cagaagaaaa aatacttggt aaaacacagt ggggaagtga
     7081 tccgctggtt gaacaagtag atgcactgac agatatcctt tactttactt atggttcttt
     7141 ttcattacta ggaatagtgc ctgatcgaat attcgaaatc gtccatcagg caaatatggg
     7201 gaaattattt ccagatggaa aacctcatta tgaccctgta acaaataaag tgttgaaacc
     7261 tgaaaactgg cagatggaat atgctccaga acctaaaatc gcggcagaaa tccaacgtca
     7321 gattcaagaa gcgattgcta agaagaaatc cgctgatata ggcatgtgca caaatgaaaa
     7381 agagtgatca gagtttcact atttttgtta tctttatacg atggaaaaac aaaagatttt
     7441 cgtttaagtt agtgaatgca tgaaaatagt atgctataat ctagaataga tgtcttttgc
     7501 acgagagacg tattatttgg atttatgcca agcagaacgc catctgttgc ataaaagatg
     7561 catactgaaa acggaggaac cataaatggg aaataatgtt atcatcggaa tcgtcattgc
     7621 aatcattata gtcgccattg tcgtcttttt ggttagttac ttgatgaaga aaaaaaacca
     7681 agatcgattg gatgccttag aaaaacgcaa ggaagaatta tttgacttgc cagtgattga
     7741 agaagtcgat gatgttaaaa aaatgcatct ggttgggcaa agccaaaata cctttcgtga
     7801 atggagtcaa aaatggaatg acatatctac ttcctcgttc gctgaactag aaagtcaaat
     7861 tttcgaagtg gaaaacctca atgaaagttt tcggttctta aaaggaaaaa aagcagttgt
     7921 tgaagcagaa gctacaatgg accaaatgga gaaacaagtg acggaaatcc gtgaaggtct
     7981 aaaggaattg cgggaaacgg aagaacgcaa ctcactagct gttcagcagg cacttgacgt
     8041 ctatgaagaa ctgaaagaaa aagtcagaga agacgagcat gaatacggac cagctctttt
     8101 tgaattgcaa aaacagatca aagtgatcga aaaagaattt acgcagtttg tgactttaaa
     8161 cacttctgga gaccctgttg aagcccgcga ggttttggat aaagcagaaa aacatacgta
     8221 tgaagtggaa gctctgatga aaaaaattcc accgctgtat gaggagctcc ataccacttt
     8281 ccctgaacaa cttgaagaaa tcagtgatac ttacgagaaa ttgatggaag aacaatatgt
     8341 attcccagaa gaagatcttg ctgaagatat cgcaaaagta agtcgaagaa ttgaaaattc
     8401 attagccaat cttgaaaaaa cagaagttga aacggtcgaa tttgaaaacc gtgaaacagc
     8461 tgatttgatt gattcgttat atgatatctt agaacgggaa atggaagctc agagatatgt
     8521 aaaaactaac caatcaacga ttgcagagta tatcaaacat acaacaaaga ataaccgtca
     8581 attattgatt gagctggatc acacatctca aagctataca ttgaaccaca atgaaattgg
     8641 ccgtgtacga ggtttccaaa ccgaagtgga agaaatggaa cgtcaaaatg aacagatgat
     8701 tccacagatc cgtcaacacg aaattcctta ttcagaagtg cgcacgttct ataaaacagt
     8761 cttcaaagtc ttagaagata tcgaaaagca acaagtagag atcgacgact cattacatga
     8821 attgagaaaa ggcgaaaaag aagcgcaaga aaagatcgat acattcgaat tcaaattgcg
     8881 cagtttgaaa cgttttgtag aaaaacaacg tttgcctggc ttgccgaatg attacctaga
     8941 attctttttt gttgctactg accgtattga agaactaagt gttgtcttga ataaaattcg
     9001 agtaaacatg gaagaggtca atcgtttagt tgctctatgt gaagaagatc tagaattatt
     9061 agataagaag acacatgatt tagtagatgc agcagcatta acggagcaaa tgctccaata
     9121 tgcaaatcgt tatcgtcata cacacgaaga agtgagagaa gcaattgata aaagctacta
     9181 tttattcaat aaagaatatc actatcaaga agcattagac gaaatcggta cagcattaga
     9241 gcgagtagaa cctggtgctt tcaaacgcat cgaagatttt tattttaatc atccagactt
     9301 agtataaagc aaaaaagtta gtaaaccagc aagagcaaga cggattattg tcttgttctt
     9361 gtcttttttt gtatatttga aaattgaaac aagtgaaaat ttcacctgct cttgaaagca
     9421 ccttggacaa ccgttataat agaaaagaat ttcaaaaaag aggggtttct tatgatatat
     9481 tttgataaca gtgcaacaac cccaatcttt ccgcaaagct tagatgcata tgtaaaaacc
     9541 agtcagcgga ttattgggaa tccatcaagc ttgcatgatt taggcaatca agctaaccgg
     9601 ttgttgcagc aagcaagaaa gcagatcgct gatttgatcc atgtagaacc agaagaaatc
     9661 tattttactt caggcggaac agaaggagat aactgggtat taaaaggaac gatgatcgaa
     9721 aaaagagaat atgggaatca tatgatcatt tcaggagtcg aacatccggc cgtcagcgag
     9781 acagcagaac aattaaaaga attaggaatc gaagtaagta tcgccccagt ggataaacgc
     9841 ggatttgtac gtgtcgacga attgaaagaa ttaataagaa aagagacagt attagtctct
     9901 gttatggctg tgaataatga agtaggatcg attcagccca tccaggaaat cagtgatctt
     9961 ttggatgatt ttcctaaaat tcatttccat gtagatgctg ttcaggcgat tggaaaagta
    10021 ccattctcgc aatggttgac agaaagagtt gattttgcaa cattttcagc tcataaattc
    10081 catggcccac ggggaactgg tttcatttat tggaaaaagg gtcgtcgttt agcaccatta
    10141 ctgacaggtg gagggcaaga aagaaatcag cgaagtggga cagaaaatgt tccaggtatt
    10201 gtggctatgg ttaaagcttt acgtttatca ttagagataa aggaacaaaa acctgagcag
    10261 acacaacgct taaaagtagc tctaatcaat gcgttgaaga cgtatgagaa agtgacgatt
    10321 ttttcagaag gaactgacta tgcaccgcat attttatgtt ttgctctaaa aggaattcgc
    10381 ggcgaagttc tcgtccatgc cttagaagaa aaacaaatat ttgtttctac tactagtgcc
    10441 tgctcctcac gtaaacatat ggcaagcagt acattgcacg caatgcatgt accaaatgat
    10501 ctggctacat ctgctgttcg aatcagctta gatgaaaaca attcgcttgc agaagtcgag
    10561 caatttatga ttattttcaa gcagctttat aagaaatttt ctgcaatcaa ttaaaagagg
    10621 agtgttcttg tgaaatacag tgaaattatg gtccgttatg gggaactttc gacaaaagga
    10681 aagaatcgaa aaagctttat catgcaacta gcacaaaatg tcagacaagc tttgaaagac
    10741 tttccagaaa tcaaaatcca tgctgatcgg gaccggatgc atcttttatt gaatggcgca
    10801 gacgggcagc tagttattcc aaaactagca aaaatatttg ggatccaaaa tttctctcca
    10861 agtattcgtg tagaaaaaga tgtcgaagtt ttgaaacaag ctgttcaaga catcatgaaa
    10921 gaaatttata ctgggaaaga aacatttaaa attatcgcta aaagaagcga tcaccaattt
    10981 gaattgacaa gtaatgaatt gaatcaaaca ttaggtaatg ctgtttttga tattttcccg
    11041 catatccaag ttcaaatgcg tcaaccagat attccattac gtgtagagat tcggcgtgat
    11101 ggcgcttatc tatcgtatga aacgatcaag ggagctggag gattgcccgt aggaacaagc
    11161 ggaaaaggaa tgcttatgct ttctgggggg attgattctc ctgtagcagg gtactttgcc
    11221 atgaagcggg gagtggaaat tgaagctgtt cattttgcta gtccgccgta tacaagtgaa
    11281 caagcactgc aaaaagcaaa agatctaaca gccaaactag caccttatgt gggaacgatc
    11341 cattttatcg aagtgccatt taccgagatt caagaagaaa tcaaaaagtc agtgccacaa
    11401 ggatattgga tgacaattac tcgtcgaatg atgttgcgtt tgaccgatga aattcgccgt
    11461 attcgtcatg gtttagtcat tttgaatggt gaatcattag gtcaagtggc ttcacaaact
    11521 ttgcaaagta tggtggctat caatgaagta acgaatacgc caatcattcg gcctgtagcg
    11581 acaatggata aacttgaaat cattgaagta gcagaacaga tcgatacatt taatctttct
    11641 atccagcctt ttgaagattg ctgtacgatt tttgctcctc cacagccaaa aacacgccct
    11701 caactggaaa aggtccatca atatgaagag cgtttagcaa tagatgaaat gatccaacga
    11761 gcactagctg gcttgaagat ggaagagata caagcagaat cgtctaatga aaaaactgaa
    11821 gaattttcag aattgctata agaggttatg aaaatagtgt tttgaccaga gtattagaga
    11881 aacaaaacga aaaaatagct ccatgtattt taccgttttt gaactaatgt cgaagattag
    11941 ctgattgaat accatttatc aagaagttat ggaaaagccg gttctttttt actaactgaa
    12001 aaataaaatt ttatttagag atcaagctac agttgataaa tgatataaaa aagagaattt
    12061 cccatgaaat agcgaggttg tgacagaagt gggcagcttc aagaaataag aaggaattta
    12121 cgaaaattgc ttttcaaatt tttgtgaaat ttcggcttat ttttcgaaga agttacttct
    12181 ggaacaccgt gtatcagagg ttgcgaaaaa gctgccctgc atcaagtaat aagaacagcc
    12241 aaacaaaaat agttcctcat attttacgtt tggttgagct tattaccgca gatgcaagct
    12301 tttgaacacc gttttatttg tctcacaccc ttttccttcc tacttgtaac cttgctcccg
    12361 tgttagaaac atgttacact aaatatgtga aattattaac aaggagcgtc gttatgcgag
    12421 aaaaaacaga tacactgcca aaagcaactg cgaaaagatt acctttatat ttacgctact
    12481 tgaagttgct ggatgactca ggtataagta gaatcaaatc aaatgagttc agcgaaatca
    12541 cccaaattcc ttctgcaacg atccgcagag acttctcgca attaggcgag ctaggcagaa
    12601 gcggatatgg ctatgatgtc ccttttctta tcgatgtatt caataatatc cttaatacta
    12661 aagaagaaaa acgaatcgca ttagtaggat atggaaatct tggcaaagca ttgaaacaca
    12721 acaattttag aagaaatgaa aatctaaata tcgtgtgcgt ctttgataat gatccagcac
    12781 tgatcaatcg agtaattgat ggagaaatga tctatccgat tgatcgtttc gcggagatag
    12841 ccaaagcaaa aaatgtgaca gtagcaatct ctacggtacc aagtaaatat tcacaatctg
    12901 ctattgatga aattgtcaaa ggaaatgtaa ccgctatact taattttgcg cctgacagag
    12961 taactgtccc agcttatgtc aatgttcaat acattgattt gacaaccgag ttgcagacgc
    13021 ttatttactt tgatgagaat tactcagaag ttttttctta ggaaaatttg agtaggctta
    13081 ttgatcttgc gagaactcgt cgtaattctt tcaaaaattt tttagtacta aatgaagtgg
    13141 ttttttcacg accacttgtt gactaaaaaa aaaaaccatt gtagaatgaa gcataacgct
    13201 gaacaagagg agtaactggc acattcattc ttagagagag aactatctgc tgaaaggttc
    13261 tcaatgacaa ccagtgaagg tagcttggga gtttctgtct tgaatggagt agagatagac
    13321 gagtcgtgat cgttaactca cttgagaggc aataatgatt gattattgca atttaggtgg
    13381 taccgcgtaa atcatacgtc ctagaatacg aaagtatttt aggacgtttt ttagtttaaa
    13441 ggaggaaaac agcatgacgg aagaaaataa cctatcaaca aaatataatc cgcaagaagt
    13501 tgaagctgga cgttatcagc attggttaga acaagatttg tttaaaccga gcggagataa
    13561 aaaagcaaaa ccttattcaa tcgtaatccc gcccccaaat gttactggaa aacttcattt
    13621 agggcatgct tgggatacga ccttgcaaga tatgctgatt cgtcaaaaga gaatgcaagg
    13681 atatgatact ttatggctgc ctggtatgga ccatgcagga atcgcgacac aagcaaaagt
    13741 agaagcgaag cttagagagc aagggatttc tcgttatgat ctaggtcgtg aaaagttcat
    13801 cgatcaagtg tgggaatgga aagaagagta tgcgagccac atccgcgaac agtgggctaa
    13861 aatgggctta tctttagatt acagtaggga acgttttaca ttagatgatg gattatctga
    13921 agcagtacgt aaagtttttg ttaccttata cgaaaaaggt ttgatttatc gtggtgaata
    13981 tatcatcaac tgggatcctc aagcacgaac agctctatct gatatcgaag ttattcataa
    14041 agatatcgaa ggcgcctttt atcatatgag ctatgaacta gcagatggat caggcgtagt
    14101 agaaattgcg acaacccgtc cagaaacgat gttgggagat acggcgattg ctgttcatcc
    14161 agaagacgag cgttatcaag cgctcatcgg caaaaaagtg attctacctt tagtcaataa
    14221 acaaatccca atcattgcag atgaatatgt agatatggaa ttcgggacag gtgtagtaaa
    14281 aatcactcca gcacatgatc caaacgactt tgaggtaggt aatcgtcatg atcttccacg
    14341 cgtaaatgtt atggatgatg atggtacgat gaatgaacta gcaggaaaat atgctggaat
    14401 ggaacgtttt gctgctcgta aagcaatcgt gcaagatctg aaagaaatcg gtcgattgat
    14461 taaaatcgat acgatgatcc acagtgtggg tcattccgag cggacaggtg tcgttgttga
    14521 acctcggctg tctactcaat ggtttgtaaa aatggcgccg ttagcgaaaa aagccattga
    14581 taaccaagaa acagatcaag cagtggaatt cttccctcca agattcaatc aaacgttttt
    14641 acgttggatg gaaaatgtcc atgattgggt aatctcacga caattatggt ggggacatca
    14701 aattcctgca tggtatcaca aagaaacagg tgaaatgtat gtagggatgg aagcaccagc
    14761 ggatagtgaa aactgggtac aagatcctga tgttttagac acttggttta gttcagcttt
    14821 gtggcctttc tcaacaatgg gctggccaga tacggaaagc gaagattata agcgatactt
    14881 ccctactagc actttagtaa ccggttatga catcattttc ttctgggtaa gcagaatgat
    14941 tttccaaagc ttggaattta ctggcgaacg tccatttgag aatgtcttga ttcatggatt
    15001 gattcgtgac gaacaagggc ggaagatgag taaatcatta ggtaatggaa tcgatccgat
    15061 ggaagtaatc gaaaagtatg gagcagatgc tctccgctgg ttcttatcga atggttctgc
    15121 accaggacaa gatgtccgct tcagctatga aaaaatggat gcttcttgga actttatcaa
    15181 caaaatctgg aatgcttcac gtttcgttat catgaatatc gaaggaatga ccgtagaaga
    15241 gatcgattta tctggagaaa aatcagtggc tgaccggtgg atccttactc gtttgaatga
    15301 aacgatcgaa cgagtaactg agctatttga tcgttttgaa tttggtgaag caggtcgaca
    15361 actgtataac tttatctggg atgatttttg tgactggtac atcgaaatga gtaaagaagt
    15421 actttatgga gatgatgctg tttcaaaaca aaccacccgt agcatcttag tccatacact
    15481 tgatcagatc ctacgtttat tgcatccaat catgccattc gttacagaag aaatatggga
    15541 acatattcct catcagggaa attctctagt agtggcagaa tatcctgttg tacgtccaga
    15601 gtttgatgat gaaacagctt ctaaagggat ggaagtattg aaagaattga ttcgagccgt
    15661 tcgaaatatt cgttcagaag tgaatacacc attgtctaaa ccaattacgt tgctaattaa
    15721 aacaaacgat gctaaaatcg atcaattttt agttgaaaat acaagctata tcgaacgctt
    15781 ctgtaatccc gaagagttga cgatatcaag tgagatcact gcgccagatc tagcgatgtc
    15841 agctgttctg acaggtgcag aaatcttctt gccgttagca ggattgatca atattgaaga
    15901 agaaatcaaa cgattagaaa aagaactgga aaagtggaca agcgaagtaa aacgtgtcca
    15961 aggaaaactt tctaatgaac gttttgtttc taacgcgccg gaagaagtag tagaggcaga
    16021 gcgtgcaaaa gaaaaagatt atttagagaa gcaaacggct gtcaaagaac gaatcgaaag
    16081 tttgcgtact attcaataag aatagaaaaa gagagtgtga aatcagctag ctattactaa
    16141 aagctagctg attgaacatc gttacaggaa agagtttgag cctcactctt agcttcgatt
    16201 cataaggaga tgtgacataa attttgtcgc atctcttttt tagtctactc ttattatgta
    16261 ggagaaggag ggaggtggat catgtatcaa gagtcaataa agtagttgat aaaatcgttc
    16321 ctctctctgt ttcttttctc tttgtcatac ttttattttt cttatgtgtt ctatttgtta
    16381 taataaaaga gaaatggggg aattgttgat gacgattgaa gaagcaatag aatggatcca
    16441 tagtcgatta ccatttggtt cacgtcctgg ccttgaccga gtcgaagctt tattagaagt
    16501 ggtagggcat ccagagagaa aagtaaaaac gatccatatc gctggaacaa acggaaaagg
    16561 ttctactgta gcttatttga gaagcctatt agaagaggct gggctgacag taggtacatt
    16621 tacttcacct tatatcgaaa cgttcaatga acggatcgca atcaatggca aaagcattcc
    16681 agacaatgat ttgatccagc ttgtaaaaaa atatcagcca ctgatcaaac aacttgatca
    16741 atttgaagca gtagctggta ttactgaatt cgagacatta acagcaatgg catttgatta
    16801 ttttttgatg aagaaagtgg acgtagcgat tatcgaagtt ggcttaggcg gcttattgga
    16861 ttctaccaat gtagctgtac caatgctgac gggaataaca acgattggac ttgatcatac
    16921 cgaaatactt ggagatacaa ttgaagaaat cgctgcacaa aaagctggaa tcatcaaaca
    16981 aggcgttcca gtagtaacag gaaatattgc cccagaagct tttactgtca tccaacaaac
    17041 agctaaagca aaaaatgcac cgctttatgt atacggggaa aattataaaa taaactatct
    17101 gcatcctgat gagatgtggg gagaagtatt tgaatttttt aatgaaatag gaaagatccc
    17161 tcaattaacc actccgcttt tagggcaaca tcaagctgaa aatgcaggag tagccatcga
    17221 attgtatttt gtttactgtc aattggaaaa gctgcctttc cgagaaaaag atgttttgaa
    17281 gggattgaag aaatctcgat ggccaggaag aatggagcgt ttgagtaacg agccattagt
    17341 tgttttagat ggtgcccata atacgcatgc aatgaaccgg cttgttgaga atttgaaaaa
    17401 agaattcaaa ggatatcaaa tcaacatttt gttttccgcc ttacgaacta aaaatgttag
    17461 agaaatgcta gaacgattgc agcaagtacc aaatgtccat atttatttga cgacctttga
    17521 acatccaaaa gccatcgaat taagtgaagt atcagattta gcaagtgaga gagtatcaat
    17581 cgtttcattg tggcaattcg gattagcaga tatattggaa aagatgacaa atgaagacgt
    17641 attagttgtg accggttcac tttatttcat ttcagaagtt cgccatttat taatacaatt
    17701 aggaggagat catgaagtat aatgcagtga tttttgacat ggatggtttg ctgttcgaca
    17761 cagagttagt gtattacgaa gccacacaag tagtagcaga caagatgggt tttccttatg
    17821 ataaagacct gtatttaaaa ttcttaggtg tttcagatga agaagtatgg gcgaattatc
    17881 atatgatttt ctcggaattt ggcaaaacaa gtgtacaaag attcatagat gattcatacc
    17941 aggaaacgct tgaacgtttt tcgcaaggag cggtacaaat gaagcccggt gtgttggaac
    18001 tcctctcttt tttagaagaa catcagattc ccaaagttgt cgcttccagt aaccaacgag
    18061 cagtcattga acttttattg aagaagaaca atctgtttga acgatttgag acgattgttt
    18121 ctgcagaaaa tgttcaacga gcaaaaccag acccagaaat tttcttgtta gctaatcaga
    18181 agctaggtac agcgaagaaa gaaacattga ttttagaaga ttcacaaaat ggtattttag
    18241 cagcttctgc ggctgagatc ccggtagtca tggtaccaga tctgcttcca ccatcccaag
    18301 aattgatcaa aaaaacagct ggggttgttt cttctttaca tgaaatccct gcattgttaa
    18361 aataaaaatc catctctttt cgtatcttat tttagataag atagaaagga gatgtttttt
    18421 tgggaaaacg attgatcaaa gaagtaccta ctagctcttt accaagggaa cgtatggaaa
    18481 tttatggcgc ggaagcttta tcggatcagg agcttttggc aatcttactg cgtaccggtc
    18541 aacaccctta tagtgtgatg tcgattgctg gaaatctgtt aaaaacgttt ggaggactgg
    18601 cctctttacg acaggctact ttgcatgagt tagaagagat tcgagggata ggacgcgtga
    18661 aagcaatcga aatcaaagcc ttgatcgaat taggtcgacg aattcagacg gatcatgaaa
    18721 gagcggctcc accggttcgt accagctacg aattggccca gcaattgatt ctggaaatga
    18781 aagatcacaa acaagaacat ttggtttgta tttatttgga cacgaagaat caagtgatct
    18841 taaaaaaaac agtgttcatc ggttcgctga atcagtcagt cgctcatcct agagaaattt
    18901 tccattatgc tgtacgttat tgtgcagcaa ggatcgtttt agcgcataat catccttctg
    18961 gtaatgttat tccatcgcag caggatatga attttaccaa aaggatacaa aagtgtggag
    19021 aaatgatggg gatcactgta ctggaccact tgatcatcgg acgcaaacga tattttagtt
    19081 tgagagaaga aggaatgatg gaagagaagt aagttgcttt cttgcaaaaa aaatgaagca
    19141 agtgtacaat tatattgtaa ttttgtttgg gaatagaagt tgaaacatct gttgcacctc
    19201 tatcgtacac gagcaatgtt acaatttaaa ttagtgcttt tggcacgagg aggatattat
    19261 tatggaaaac ggaacagtaa aatggttcaa cgctgaaaaa ggatttggat ttatctcacg
    19321 tgaagatgga agcgacgtat tcgttcactt ctcagctatc caaggtgacg gattcaaaac
    19381 attagaagaa ggtcaagcag tgactttcga cgttgaagaa tcagaccgcg gcccacaagc
    19441 tgcaaacgtt gtaaaagctt aattttatct gtaaatcaaa ttgatggtag acatcaattt
    19501 ttacgagaga acaaagcgag taacaaaaat acctgccaca agagacaatt tctcttgtgg
    19561 caggtatttt tttgtatgaa aaagtaaata aatttaagag gctatgaaag agccaccttg
    19621 tatcaaggaa taagaacaaa caaaaatggc ttctcatgtt tttcatttgt tttctcttat
    19681 attcaggaca aggtgctgat ttcataacct cttatttatt cttcatctgg tgtcattacc
    19741 attggcaaaa tcattggatg tctttctgta cgttcgaata agaaaggctg aagcgcgcca
    19801 gtcatcgctt ctttcaattt atattctgaa cagtcaggtc cattcaaaca ttctcttaaa
    19861 gcgtggaata agattcgctg agcttcattg atcatttctc ctgattctcg catgtagaca
    19921 aatccacgag agagaatatc tggaccagcc aagatttctt ttttcttgat atccactgtc
    19981 gcgacagcta atacaagacc ttcttctgaa agcaggtgac ggtcacgtaa aacgacatta
    20041 ccaatatcac cgatcccgct accatcgata tacacatctc cagccacaaa atgcccagca
    20101 ggacgcgcag aatcgctagt taacgctaaa acatcgccat tttccatgat gaagcagttt
    20161 tctgcaggaa cacctacatc ttgtgctaga gaagcatgta ctttcaacat gcggaattct
    20221 ccgtggacag gaataaaata ttttggcttc atcaaacgaa gcattaattt ttgttcttct
    20281 tgtccaccgt gaccggaagt atggatgtta ttgatttttc cgtgaatcac ttctgcacca
    20341 gcttcagaca gcaaattgat caaatgattg acacttgttg tattaccagg aataggtgaa
    20401 cttgaaaaga tcactgtatc gtctggctgg atttggattt gacggtgagt accattcgcg
    20461 atgcggctca acgcggccat cgcttctcct tgagaacctg tacataggat catcgtttca
    20521 ttggcaggca agcgattgat ttcgtgtgca tctacaaatg tgccgtctgg aactttgata
    20581 tatcctaaac gttgaccgtt gacgatggca ttttccatgc ttcgtccgaa aacagcgatt
    20641 ttacgaccag ttgccacagc ggcatctgct gcttgctgca aacggaagat gttagaagca
    20701 aagctggcaa aaatgatacg tccttcgatt tttgaaaaga tcttcaaaat agaatgccca
    20761 atcgtttttt ctgatttagt aaatgttgga atctcagcat ttgtactatc agataaaagg
    20821 cacaagactc cttcttcccc gatacgagcc attttatgca agtttgctgg ttcgccaaca
    20881 ggagtgaaat cgaatttgaa gtctccagtc gctacgatat ttccagaagg tgttttcacc
    20941 acgatcccta atgcatctgg gatactatgg gtcgttctga aaaagctgac agaagtcttt
    21001 ctaaaacgga tgatcgtatc ttcattgatc tcatggagct ctgcatctcg aagcaagcca
    21061 tgctcatcta atttgtttgt gatcaacgct aatgcgagag gaccagcata gataggaata
    21121 tttgcttgtt tcagcaagta gggtattccg ccgatgtggt cttcgtgccc gtgagtaatc
    21181 accaaggctt ttactttgtg taagttttgt atgatgtagc tgtagtcggg aatgacatag
    21241 tcaattccaa gtaaatcgtc ttccgggaat ttgatcccgg catcaatcaa aatgatttca
    21301 tcttggaatt gtaccccata cgtatttttc ccgatttcac ctaatccacc aatagcaaag
    21361 acgcctgtct cgttattttt gatggaaagt ttcatattaa aactccacta atgagaaatc
    21421 agcattttct ttttcgtact ctagatgact tccttctaga agttgaacga attcaatatt
    21481 gtatggggta ttttcttcca cacgttggcg agcgtctaca tcgctttttg cctcaatgta
    21541 aagagatttt gtttcttccc gtttagggtt gcgtactttt gtttcttgat aataaacttt
    21601 atagatcatt gttttgtctc ctttatctgc gtaaagcagt gatttagcta gttgattttt
    21661 ttagacaaca caaaacgagg ggacaagcca cctggttttt catatgcttt gactagaata
    21721 ctggattcga gtcaaagaga caagcctcgt ttttgcttat ctgttcggat attttttcaa
    21781 ccgtaccttc tgtataaaaa atatacaaca attaattgtt attctagcat aaaacagcaa
    21841 ataagtatag aaatcaacta gattgaaaga aaaaatcaat gtttatcatc ccgctataat
    21901 cataatgact tttttatgag agaagtgttc tattaatagg ctcgttgtgc tatcattttt
    21961 agtaatgaaa atgtaacaaa agattaaaag gagatgagaa agtgaaacta atgtggcatt
    22021 ataccatgcg ttataagaaa ttcttgtttt tcaatttcat atgcgtattt ggttttattt
    22081 tgattgaatt aggacttcct acgattttag cacgtatgat cgatgtagga atccgcaatg
    22141 atgattttga ttatgtgaag caacaaggga tatttatgat cgttattact gtaatcggta
    22201 ttattttgaa tatcttgtta ggttattttg gttcgaggat tacgactaat atcgtagcag
    22261 atattcgaga tgatttgttc gaaaagatcc aaagttattc gcatcaggaa tacgaaaatc
    22321 taggcgtatc ttcattgatc actcgtacaa caaacgatgc ttatcagatc atgttgttcc
    22381 tgcaaaatat tttacgtatc ggttttatgg caccaatgat gttcattgtc agtttgtaca
    22441 tggtcatgcg tacaagtgtg acactcagtt tgtatgtagt aggtgcatta cctttactat
    22501 tattggcagt cgtagcgatt gctaaggtgt ctgaaccgtt atctaaaaag caacaaaaaa
    22561 acttagataa aatcaacagt atcttgcggg aaaatctttc aggattgcgt gtgattcgtg
    22621 cattcgtcaa tgaaaagttt gaagaaaaac gttttgctgt agtcaatgag aattatgctt
    22681 ctagttcaaa aagcttattc cgtttgatgg ctgttgcaca accgggtttt tatttcttat
    22741 ttaatatcgt aatggtattg atcatctgga gcggggcagt gcaaatcgat gcaggcgagc
    22801 ttcgtgtagg agatttgatt gcgtttatcg agtatatatt ccatgccttg ttctcattca
    22861 tgttatttgc tagtgtgttc atgatgtatc caagagcagc tgtttcagct ggacgtatcc
    22921 aagaagccct agatgccaaa ccttcgattg tagaagatcc aaatggtata tctgatacaa
    22981 aaactaaagg gtatatcgaa tttcaaaatg taacatttgc ttatccaggc cattcgcaag
    23041 agccagtgat acgaaatgtc agttttacag cctctccagg agagaccgtt gcttttattg
    23101 gaagtaccgg tagcggaaaa tctacgttga tccaattgat ccctcgcttt tatgatgtga
    23161 gtgaaggaaa gatcttgatc gatggagtag acgtgagaga ttatcggcta agcaagttgc
    23221 gagataagat tgggtttatt ccgcaaaaag cgctgctatt tactggaacg attgcagata
    23281 atttgcgata cgggaaagaa gatgctacgc aagaagaaat ggaacgtgca gctgagattg
    23341 ctcaagcttc agaattcatt tctcgaaaac cagatggatt taatgaacat ttatcagaag
    23401 gtggttccaa tttttcaggt ggacaaaaac aacgattggc aatagccaga gccatcatac
    23461 gccgtccgga aatctatatt tttgatgaca gcttttctgc attagattat caaacggatg
    23521 cgaagttacg tgctcgtctg aaaaaagaaa cgacagaatc tactgtattg atcgtagcac
    23581 aacgtgtggg aacgatcatg catgcagata agatcatcgt attaaatgaa ggagaagtcg
    23641 tcggtatggg gacgcatcgg gagttgttgg aaaaatgccc gatttattat gatattgccg
    23701 cttctcaatt atcaaaggag gaattagcat gaaaaaaacc ttttcttcct taaaacgttt
    23761 agctcgatat atcagaccgt atcgtgagac ttttctttta gtgatcctat ttacagttct
    23821 gacagtggct ttcaacgtgg ctttgccata tgttacagga cttcctacta cagaaatcag
    23881 ccgtaatatt gccaatggcg aatcaatcaa tttttcttat atattcaaat gtctcatttg
    23941 gatcacagtt gttggaatag ggtattgtat ttcgcaatta ctttctggtg ttttgatgac
    24001 aaatgtcgtt caaagtgcca tgcatgattt gagaagagac atagaagaaa aaatcaatcg
    24061 tcttccggtt tcatatttcg ataaaaacca gcaaggaaat attttgtctc gtgtgacgaa
    24121 tgatgtagat gcagtcagcg gtgctttaca acaagctttt attggtatcg taaatgcgat
    24181 attgggaatt acgttggctg cagcaatgat gttttatatc cagccgatga tggcgctgat
    24241 ctctatgatc atgattccaa catcgatctg gatctcaaaa cgagtgatca atgcttcgca
    24301 gaaatatttt caaagcatgc agaattctct tggtgagcta aacggctacg tccaagaaaa
    24361 tatgacaggt tttagtgtat tgaaagtata tggacgcgaa aaagaaacat tggaaggatt
    24421 caaaaaagtt aatcacagct tgaaatactt tgggtttcgt gcagcattca tttctggctt
    24481 gatgatgcct ctagttcaat taactgccta tgctacttat attggtatgg ctgttctagg
    24541 aagcttttat gttattactg gggcgatcgt tgtcggtcaa ctacaagcat tcatccaata
    24601 tatttggcaa atcagtcagc caatggggaa cgtgacacaa ttgtcttcag ttttgcaaag
    24661 tgcatcagcg gcaacgaaac gtgtattcga gattttagat gagccagaag aaaaagaaaa
    24721 caaagtagat gcttcactgc cagaaacgat tttaggagat gtgacattcg atcatgtcga
    24781 ttttgcatat gatccgaaga aaccattaat ccaagatttg aattttgaag tgaaagctgg
    24841 tcaaaccgta gctgtggtcg gtccgaccgg tgccggcaag acgacattga tcaatctatt
    24901 gatgcgtttt tatgacgtag acaaaggagc aataaggatt gatggaatcg acaccaaatc
    24961 aatgagtcgc agtgatgtgc gttcgctatt tggtatggtc ttgcaagatg catggcttta
    25021 tcaaggaacg attgcagata atatccgctt cggaaagctt gatgcgacag attatgaagt
    25081 agttgatgct gcgaaaacag caaacgtaga tcattttatc cgaacgatgc cagatggata
    25141 tgatatggcc atcaattctg agggagacaa tgtgtcttta ggacaaaaac agttgctgac
    25201 gattgcccga gcagtggtct ccgatcctaa aatcttgatt ttagatgaag caacaagttc
    25261 agtagacacc cgtttagaag cgttgattca aaaagcaatg gatcgcgtga tggagggacg
    25321 gacaagtttc gtcatcgctc accgtttatc gacaatccgt gatgcagatc tgattctagt
    25381 gatggatcaa ggtcagatca ttgaaaaagg aacgcatgag agtttattgg cacaaggtgg
    25441 attttatgaa aaactgtata acagccaatt tgctgaggaa gcagaataag tcatataaga
    25501 ataaaacagt ttaaaactta ttttttgttc tctattcttg ataatggtaa agtaaagtta
    25561 tcaagaaaag tgaggacacg gagatggaac agtttttaaa tgaaaaaatt actgaagcca
    25621 aaagagaagt tgatcgttta acgcacgtct gtcagacgaa gccttttgat tcagctgatt
    25681 tttttgaaaa tgagtttcaa atcgaacgct tggcagcaca aatagaagtg tacgaat
//
LOCUS       DACCYH010000035        25503 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.035, whole genome shotgun sequence.
ACCESSION   DACCYH010000035 DACCYH010000000
VERSION     DACCYH010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.035"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            47..2107
                     /locus_tag="IVY85_001386"
     CDS             47..2107
                     /locus_tag="IVY85_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044411.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2190..2759
                     /locus_tag="IVY85_001387"
     CDS             2190..2759
                     /locus_tag="IVY85_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8044412.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKSDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2934..3740)
                     /locus_tag="IVY85_001388"
     CDS             complement(2934..3740)
                     /locus_tag="IVY85_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044413.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3878..5392
                     /locus_tag="IVY85_001389"
     CDS             3878..5392
                     /locus_tag="IVY85_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP8044414.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5795..6778)
                     /locus_tag="IVY85_001390"
     CDS             complement(5795..6778)
                     /locus_tag="IVY85_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP8044415.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6783..7430)
                     /locus_tag="IVY85_001391"
     CDS             complement(6783..7430)
                     /locus_tag="IVY85_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8044416.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7515..8147)
                     /locus_tag="IVY85_001392"
     CDS             complement(7515..8147)
                     /locus_tag="IVY85_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8044417.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8163..8714)
                     /gene="coaC"
                     /locus_tag="IVY85_001393"
     CDS             complement(8163..8714)
                     /gene="coaC"
                     /locus_tag="IVY85_001393"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP8044418.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8711..9448)
                     /locus_tag="IVY85_001394"
     CDS             complement(8711..9448)
                     /locus_tag="IVY85_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP8044419.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9445..10434)
                     /locus_tag="IVY85_001395"
     CDS             complement(9445..10434)
                     /locus_tag="IVY85_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8044420.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10692..11234
                     /locus_tag="IVY85_001396"
     CDS             10692..11234
                     /locus_tag="IVY85_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8044421.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11251..12336
                     /locus_tag="IVY85_001397"
     CDS             11251..12336
                     /locus_tag="IVY85_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8044422.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12348..13154
                     /locus_tag="IVY85_001398"
     CDS             12348..13154
                     /locus_tag="IVY85_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8044423.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13155..13982
                     /locus_tag="IVY85_001399"
     CDS             13155..13982
                     /locus_tag="IVY85_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8044424.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            13979..15052
                     /locus_tag="IVY85_001400"
     CDS             13979..15052
                     /locus_tag="IVY85_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8044425.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15480..18176
                     /locus_tag="IVY85_001401"
     CDS             15480..18176
                     /locus_tag="IVY85_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8044426.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18253..18741
                     /locus_tag="IVY85_001402"
     CDS             18253..18741
                     /locus_tag="IVY85_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8044427.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18753..19247
                     /locus_tag="IVY85_001403"
     CDS             18753..19247
                     /locus_tag="IVY85_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8044428.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19268..20077
                     /locus_tag="IVY85_001404"
     CDS             19268..20077
                     /locus_tag="IVY85_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8044429.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20074..20895
                     /locus_tag="IVY85_001405"
     CDS             20074..20895
                     /locus_tag="IVY85_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8044430.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            20946..23045
                     /locus_tag="IVY85_001406"
     CDS             20946..23045
                     /locus_tag="IVY85_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP8044431.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23042..24160
                     /locus_tag="IVY85_001407"
     CDS             23042..24160
                     /locus_tag="IVY85_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP8044432.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24199..25077
                     /locus_tag="IVY85_001408"
     CDS             24199..25077
                     /locus_tag="IVY85_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8044433.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25268..25420
                     /locus_tag="IVY85_001409"
     CDS             25268..25420
                     /locus_tag="IVY85_001409"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044434.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         8058 a         4439 c         5413 g         7593 t
ORIGIN      
        1 aattactact tcatgtttag acaagagtag ggaggactaa gaataaatgt gttcaaaaag
       61 acgttcaaaa caactaaata aacacgcttg tcgtttagat aaggaacggt cgtgttctga
      121 aaaacactac gttaagttta tgatgcgcac gatgctgatc agtttatctg taggtgtcta
      181 tatgcatgta cccgttcagg caacgacaca aaccatcaag gtcgtacgta cctctgtttc
      241 tgagaccact gattccccta tcactattac tcatactttc actgatgcaa atggaaaaca
      301 gctacttcct gcattgactt cctataataa agtcagttat cctccaacga taaaccacta
      361 ctggttaatt cctaacaaaa cattatggac atcggaaacc ggaagcagtg tgccgctttc
      421 tatcttttcc gatatgactg atcctacaaa aggtgatcag ttaaaacaga tcactgatat
      481 tttgaatcat gctttgacag atataaagga cggtggaagc gcacaagttt cttatgtata
      541 tgaaaaagat tattcacagt ttgaaagaaa gaaaatcgat gttctggcag ggacaaaagt
      601 ttcgatatct gatctggtag aaaggctgaa gaacgcagat ggaacacaag gagatatgtc
      661 caaagtaatt tcaaacaatg ggaatatcgt ggatacgact aatacaggaa catctactat
      721 ttccttgtcc tattttgatc caattgcttt aaaaacaatg actgcatctg ctgtgatcca
      781 tgttcgtgct gatcaaacag ctatcaaagg aaaagatctt cacttaagaa caggagagag
      841 ttacgcccct aaagatcttg ttgaatatgt aacaaaaagc gatggaactt caggaaataa
      901 agaagaaatc aaaatgaacg ggacagtaga tactacaact ccgggagaat atcccgttgt
      961 attaacttat atagactcga aaacatcaaa tgtggcagaa acaacagctg tagtttatgt
     1021 agaagcatct gctgcttcat cgactgtcat cactcatttt atagatagtc aaacaaataa
     1081 agaacttcaa gcagaaacac atatgagtag tgtaaatcgt attcctgatt tgcgcctacc
     1141 tgctgaattt tctgatcgag tgccaaatat agatttatcg acttatgaac aaaatgggaa
     1201 agtcacttct atttctgatt ggatgcaagc aaacggatta gacagaaaaa gtcactggtc
     1261 ggagatcctt gaagtacttc actcagaact gatgaataat aggaagcaat caggagggga
     1321 aattaccttt acttatgttt atataccaga caatcgttca ttagaaggtc atgatcttat
     1381 tgttcatcca aacgaaaaaa taaacaaaga agagttgatc caatcagcaa caaattcctt
     1441 aggtaaaccc gtaccgttaa aggatgtatc ggttacaatc aatgaagtac cattggagga
     1501 tggttatttg accaaacaaa caggtgattt tacgattatg tatagttatc atgacccgta
     1561 ttcactccaa gaactccatg ctcagtcact attacatgtt ttaccattaa acaaacagat
     1621 agatcaaaaa gaacaaaaaa gacaagaaga aagcttgtca gaaaaaaaac aacaaatggc
     1681 tcatgcagtt tctccaacag ggaaagtaaa acaaacaaga agcctagagt tagcaagaat
     1741 atctagaaaa atctcagaat ctaataaaac gataaggcaa gaagctcatt tggccccaca
     1801 agtttctgga ttggaaaatg aaattttaga gcctaaaata aaacaacaat cagaaaaaga
     1861 atttgttgct aaacagtcgg aagatcagaa ggaatcagat gaccagaagg aaacgaagaa
     1921 aaaacgcttg tcaactattg attccaccga tgagcatctg cagccgcaag taccacttcc
     1981 tagtccggga acacccgcac ctggtggagg tattccttct tctgctttag gagatttcat
     2041 gcgaggtatg gcaggaacat gggtatacgc taatcgaaat gaatttgacg cagatgactg
     2101 gttctgattt atatctctat ctgttatagt caaataagta gctgagtcat atgttatttg
     2161 gcataatgga gacgaggagg gatagaaaaa tgttagataa aacgttacct tacgcagaaa
     2221 tctggatgac aagaccactt caacaaaatg ttccaatcac gcctttagcg gaaggttttg
     2281 aatttcgatt ttatcaaaaa gaagatgaat ctgcttgggc ggagattgag acttccgttg
     2341 gggaatttca aacgacagat gaagcacttg agtattttca acaaaacttt gctccttatc
     2401 ctgaagaact tgaaagaagg atgttttttg tagagacaaa aaaaggagaa aaagtagcaa
     2461 cctgtaccgc ttggcgaaaa aaatcaagag agcaagtgta tccggttttt cattggttag
     2521 cagtgaaatc agattaccaa ggaaaagggc tcgcaaaagc attgactgcc agagtcttgc
     2581 aagaatttcc taccctccat acggacggac caatctatct tcacacacag acatggagcc
     2641 atcaggcgat tgctctttat aaaaaaatgg gttttacttt tattgctgaa aatctagacg
     2701 gcacacctaa tccagattat gaaaaagtta tgcaggtttt aagtaaatta gataaataac
     2761 gaaatggtga aataagtcct cagtattggg ataaaaaacg aaatagtcat tcctcatatc
     2821 ttactttttt gagttaatac tatcggataa ctaatggcat gccatacatt caaaaaagaa
     2881 ggggcagtga caaaagtatt gtcactgccc cttctcttgt tttttagcat gttttatcgt
     2941 ttgatttttt taggaataaa attcttgatg aacgtaaatt tttggtaatc gtttcgacta
     3001 tagaggtaac caaatacact gaaaacaact gctccaataa aattaacaaa taaatccttc
     3061 atagtatcca tgattcccac gtctaagtaa ccattttgga tgattgtttt atctgtttta
     3121 ccatctgcat tttgactttc aatgattgtc cgagtgatat ctggaacatg atagacagtg
     3181 tttccgtctt tacttaactc cacactattg atttgctgga tgatcgtatc tttttgcata
     3241 tcgaattgca gtaatttatc tgctgaaaat tccacaaact cccacataac accaactgtc
     3301 atcgaaaaac agaaggaaac aactgctagt aataaaggag tcaagttcat ggtttttgta
     3361 tttttattga gcaaataaac aagggaaaag ccgacacccc cagctaaaaa tccgctgaat
     3421 gcatgtagaa aagtatccca cattgaaaaa tagccataaa aatcacttaa ctctcctaaa
     3481 actgttgaac taaaaataaa agataacaaa atgatttcta gcatatttgg aatctgtatc
     3541 tttaatatct tttccagaaa ataagggatt tcaaataaaa ttaatgtcag taataaaagc
     3601 attgtatttc cccagtgttg gttccagacc tgatccacta gcataataac gacgatgata
     3661 cgtaataaaa tatagattcc gtttgtgatt ctacctgcag tttttgtact gttcgtaaaa
     3721 aaggttttca aatgattcat aaaataactc cttcatgtat atccttaatt atactaagaa
     3781 gcgtgtgttt accaaagcta tatgacttaa agaaaaacag agttgatatt gtgaaaaaaa
     3841 tcacttaata tagtataagg agaaaagggg gatacaaatg aaaaaattag ttgctggttt
     3901 attaacagcc gttgcacttc ttgctatgag cgcatgcggc gcagatcaga aaaatacagc
     3961 gcagacaagt tctagtacga aggaatctgc cgaagtatca tcaggcgctt ctgaacaagt
     4021 atatacagat ccaaaagaaa tgaaagaatc ttatgatgtc gtgattattg gagctggtgg
     4081 tgcagggatg gcagcagcta tcgaagcaaa agatgctggg ttgaatccag taattttgga
     4141 aaagatgcct gttgccggag gaaacacatc taaatcatca agcggtatga atgcttcaga
     4201 aacaaaattt caaaaagaag aaggaatcaa agactcgaat gatgccttct atgaagaaac
     4261 gctaaaaggt ggtggaggaa cgaatgataa agaaatgctt cgatactttg tcgatcactc
     4321 tgctgatgcg gtcgattggc tagatcaaaa cggtatcaaa ctggacaact taacgatcac
     4381 tggagggatg agtgaaaaga ggacacatcg tccgtctgat ggctcggcta ttggtggata
     4441 cttagtggag gggttattga ataacgtcca tgagcgggaa atccctattt ttgtcaacgc
     4501 agatgtgaca gacattaaga aaaatgggga aacggtcaat gaagtgactg tacatgttca
     4561 aggagaagaa ccaaaagagg tcaccggaaa agcaatcgta gttaccacag gcggttttgg
     4621 tgccagcaaa gaatttattg aaaaatatcg tcccgattta gctgattata ttacgacaaa
     4681 tcaagaagga tcaactggag atggtatcac catgattgaa aaattaggtg gacaaaccgt
     4741 tgatatggat aaaatacaaa ttcatccaac tgtgcagcag gatgaggggg tcttgattgg
     4801 tgaggttgtt cgcggagaag gagcaatctt ggtcgatcaa gaggggaaaa gatttgtcaa
     4861 tgaaatggat acaagggata aagtatctgc agcaattaca gcattgcctg aaaaatcagc
     4921 ttatttgatt tttgaccaag gcgtgcgtga tcgtgccaca gctattgaat tttatgacca
     4981 aaaaggatat gtcacaactg gaaagacaat ggaagaatta gcagaaaaaa tcgatttacc
     5041 aaaagaaaca ctagcaaaaa cagttgatgg atggaatacg gatgttgcgg agaaaaaaga
     5101 tgctcaattc aaccgtgcca cagccatgga acatgactta tcgtctccga attattatgc
     5161 gatcaaaatc gcaccaggaa tccattacac gatgggcggt gtgaaaatca atacgaacac
     5221 tgaagtatta gataaagaaa accagccaat tagggggtta tatgctgctg gtgaagtcac
     5281 aggtggctta catggaaaca accggatcgg tggaaattca gttgcggaga tcattatttt
     5341 tgggcgacaa gcgggcatac aagcagctcg atttgcttcg gaaaatgaat gaaatagaac
     5401 gtaaataaaa agaggttgtg aaatcagcgt tttgagctaa taccgagggt cagctgattg
     5461 aacactgttt agtgaagagg ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc
     5521 aagtaaaaac agcttcttat gtttttaact tggttggact tattaccgca gatgcaagct
     5581 tttgaacacc gtttattcgg aaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc
     5641 acgctctaaa cacgtataaa cggcggaagc agaagaaatt ccttcggaaa taagctgaaa
     5701 ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc
     5761 ttctgtccca gcctcttttt tgatcagcat gattttattc agttatcttc aaaatgattt
     5821 ttccgatgtt tttgtttgct tccatatatt catgcgcttg tttgactgcg ctgaattgga
     5881 atgtacggtc aatgattggc cgaatcttgc cattttctag aaaaggccca gctttttgca
     5941 taaaagtttg tgcgagctct gctttatact gatcacttct aggtgtcaat aatgtaccaa
     6001 gcagttggac acgtttccca atcaatacac caaggtctat ctcaggcacg atggtcccac
     6061 ctagcatacc aatcaagacc caacgtccat caacggctat agaagctaaa ttcttttccc
     6121 agtaagaagc gccgatgaaa tccaaaataa catcgactcc ccgaccttca gtgatttttc
     6181 gtacctcttc ttgaaagtct tgttttttat agttgatcaa ttcatctgca ccaagttctt
     6241 tacaaaaggc gagtttttct tctgatcctg cagtaacgat gattttcgcg tgagaaagct
     6301 gtctagctaa ttggatcgct gctgttccca caccacttgc tcctgcatgg atcaagacag
     6361 tttcgccttc ttgtaatttt ccaagccagt aaagtgtttg ataagcagtc aaaaaaactt
     6421 ctgagattcc agcagcagaa acatagtcta gcgtatcagg gagaaggatt gcacgatcgg
     6481 caggcatcac agcatattcg gcataaccac ctagattgac taatccgtat acttttgtcc
     6541 caggagtaaa agtggagata tcacttttat tctcaactac ctctccagct acttcgactc
     6601 ccaaaatagg gtagggaggt ttcaaagaga ctgattctct tttcataata tctgttcgat
     6661 tgacagctgc tgtatggact ttgatcagta attcgccttg ttttggttta ggaacaggca
     6721 cttcaatttg ttctaataca tctacagaac caggatgctt gattgaaatg gcacgcatgg
     6781 atctattcct cctttgccca ttcttggaat gctgtattca actctttgct accttcagaa
     6841 agtactagag tcgtacgaga aatatccgtt tcttccacta gagcggcaag tacagcagca
     6901 actgtagagc gagtgatcgt ttttgatgcc ttctcattag gcttagttaa ttgaatggaa
     6961 gtaacttctt catcgttagt caatgtgact ggttgaagaa tgacaaaatc taagttagtt
     7021 cgtttcagcc attcatctgc ataatgttta gtaatataat aatctgtcat ggactcaggc
     7081 cattttgctg gttcatctgc ataaacagca ctgaccatca gatagcgaga gacatgcgca
     7141 ttttcagctg cgatcactgt tttgattgcg ccatccaagt ctacttgcag aagattcttt
     7201 ccttgagagc cggcagcaaa aatcaataca tcgatgcctt tgaatgcttc ggtcattttt
     7261 tccacggacc aagttaaatc aaaagagaca tacgaaacat tctctttttt tactaatgtt
     7321 tgggaagcaa catctctcac ccctgcaaaa atttcatggg aacttggagc taaatctttt
     7381 atcaggtgtc ggccaatttg tccgtttgca cctacaacaa aaattttcat ttggatcatc
     7441 ctttcgacgt caaatctttc cattcaatag taacaccaaa agagaaaaaa atcagagatc
     7501 ggctacgatg actatcagtt agtaagagaa gggtgcttga agtatcttaa caagacattg
     7561 catactgctg cagtcgcaag acctgcgacg attccttcag gtataccact tgtaaaaata
     7621 accgctagaa tagctgaata aacagcattt aaatttgttc cgatgacttt ggcataatct
     7681 tgctggaata aaaagtagat catattcatc actaaaatcg tattggtcaa tgaaccggaa
     7741 agtccagcta agaataaaga aacggaatct gctttttgtt tcattagttt gcgcagacct
     7801 ttataaacga aataaggaac caccccaatc aagatgcgag gaataaacgc gatcagcaag
     7861 gctttccagc ttccatgttc tgttccgatg actgggataa acggagaaaa gacaaaagac
     7921 aaaggtgttt ggataactgt gctacggatc atgctgatca gtccaaatgt accgccaaga
     7981 aaagcaccta gtcttggtcc taaaacgatc gaagcaatga ttacaggaat atgcatggtt
     8041 gttgcattga ttggaccgat agggataaaa cctaaaggcg taacagctaa taaaatcaaa
     8101 atagctaaaa acatcgcagt taatgtaaag ttttttgtat ttttcataag tactcctttt
     8161 ttctaagcat ttctttcatt aagagtcagc atgacctgtt cgatgatcgt ctgaatatca
     8221 gctaaggcac cttttccaaa atctccacaa gctaaaagag attctcttgg ttcaatttcc
     8281 tgatagccga cagatctcaa tgtttccaga ttacgctgag tgatcgggtt ttgatacata
     8341 ttcgtgttca tggcaggggc aatcaatttt ggtgttccca ctggaagcgc cattgcgact
     8401 gttgaaagaa gatcgtctgc aatgccgttt gctagcttgc cgataatgtt tgctgtagca
     8461 ggcgcgatga gaaacaagtc agcttgtttt gctagttcga tatgattgat tacctgcgga
     8521 tttgattctt gcatgacgtc tgtatggact gcgcgcttcg acagagattg caaggttaat
     8581 ggcgtgataa aatgtgtgct gcttttagac ataatcacat ctacctgggc acctaatttg
     8641 acgaattgac ttgtaagatc agcgcttttg taagcagaaa tacttccagt aacgcctagt
     8701 aagatctttt tcattttgat tcctccagtc taattttctc agtaatcaat gcagcaatct
     8761 cagatttact ttgagcttct tccactgttc catctttaga taagagatag ccgtggtgtg
     8821 tctcgccatg gatttccgtc aaatcatttg cgaaaataaa atcagttttg ttatttatca
     8881 gactattttt tgcgactgcc agaagagatt cctttggtac atctactaat agtttgaaac
     8941 caatgatttt tgcttttggc tgccattcac gtagataaga aatgattttt ggtgttttct
     9001 tcaaaacgag cacaagatgc tcggtgttag aagagatctt tgaaacagtg gtatctgtct
     9061 gttcattttc cgcaaaccaa ttgtccagtt cttcttgcgt tgaagaagtg ggtaggtttt
     9121 ttgccagctg ttcttctgaa aaactaaaag caggagtaaa atcactgact gccatactgt
     9181 ggataatcgc atcgtagtgt tcttttttcg ttagttcttc taattgtaaa aataagtcca
     9241 gtgcagattc gatcgtataa atcgttatat tggagtgtct tttcggtttc agtgcttgtt
     9301 tcgtagtcac gtaatcgatt gtggtatccg gattggcagc taaataatca gctaatgctt
     9361 gccctaatcg tcctgtcgaa tgatttgtaa tagcacgtac ttgatcgatt ttttcagtcg
     9421 ttcctccagc tgtgattaga atcttcattt atggtcacct acatttacta gtgttcttcc
     9481 ttggtgtgtt ccagcaagta gtttagatgc tgtttcaggc aattgatcca gagtaatctc
     9541 ttggacgacc agttcatctg caatctgcaa atccgccatc tgttgccata aagaaagacg
     9601 ttgatctatt ggtacattga cagaatcaat tccaatcaac tggatgtttc gcagaataaa
     9661 aggcaagaca gtcgcgttta gtttgattcc cccagcattg ccacataaag ctactgcacc
     9721 gttataagag atcaatggaa gaagactgct tagctgctcg ccacctactg tgtcgatgac
     9781 atagtcgatt tgctgttttc ctaaagcttt tgttgtctct ggcagaaatt catcaggtcg
     9841 tacgacttgc aaagcaccta gactttttag ccatgtgaca gactcttctt ttctggaaaa
     9901 ggcaatgatt gactggaatc ctagtttatg taacagggct atcgctgtac tgcctacacc
     9961 gccagaagca ccagtaacga caactttttt atcggaggtc ataccttgac ggattaaagc
    10021 gttcacacac aacatagcag taaaccctgc tgtaccaagg atcatcgctt gacgcagcgt
    10081 catatttttt ggtagaggaa ctaaccagtc tcctggtact ttttgatatt ggctataacc
    10141 gcctggatgg gtgacgccaa gcccaaaacc agtagcaagt acaagatctt cttttgaaaa
    10201 acgtggattg ttactttgga cgacagttcc ggataaatca atccctggag tcatcggata
    10261 tgttcgtatc acctgaccag actcgctcat tgccaatgca tctttataat tgacatcaga
    10321 ataggcaatt tttaccaaga cttcattttc agccaactca gtaaatggca gatcactgat
    10381 tgctgtatgg aaaacaggtt cctttattac ctgaaaagat cgaaaagcgt tcattcgtga
    10441 gacactcctt ttaaaatttt cttttatcat accataaagc ctactctttt attcttgttc
    10501 ttccgtaaat ttagacaaaa attaggtgaa aaataaagaa tcaaacaact agaatttatt
    10561 ccaaaagata aagaaatatt cagcaaaaaa aattttacaa agggttgcct ttttcaataa
    10621 atttcgtata ctaatacgag tggtagtaaa caggaagttt actattagaa aacttcgatt
    10681 cggaggaaat tatggaaatt ggtgagaagt taagaaattt gcgcatccaa aagaatttga
    10741 cacaagaaga actaggcgaa cgcacagatt tgtcaaaagg gtatatttcc cagttggaac
    10801 gagatttaag ttcaccatca atggaaacat tcttttctat tcttgaagtc ttaggtgtga
    10861 ctccagaaga attttttcat caagaagcag cgaatctgca agtggtttat tcaaaagaag
    10921 atcatacggt ttatttcgat gaagaaaacg ggtatgaatt ggaatggctc gtggctgatt
    10981 cgaatgaaaa agaaatggag cctgtcttgc tgacttttga aggtgcagga gaatataaga
    11041 cgtttgaacc ttcattatct gaaacgttta tttttgtatt agaaggggaa ttagagttaa
    11101 gcttaggaga gacggtctat gtggcaaaag caggacagtc gatctattat caagcgacaa
    11161 aacaacatca attaaggaat catctcaaaa aacgaacaaa agtattgatt gttgcaacgc
    11221 aatcatattt gtaatatagg gggtaaaaac gtggggaaac caatcatttc atttgatcaa
    11281 gtagtgaaac gctatgatga tgaaacagta ttaaaaaaag taagttttga gatcgaacaa
    11341 ggaaaatttt atacactgct tggtccttcc ggctgtggta aaacaacgat cctacgtatt
    11401 atagctggat tcaccgaagc tacagaaggg gacatctatt ttgaagggaa acggttgaat
    11461 gatttgccag ctaataagcg acaagtaaat acagtcttcc aagattatgc actttttcct
    11521 catatgaatg tttttgataa cgttgctttt ggattgaaga tcaagaaaat gtccaaatca
    11581 gatatcgaaa agaaggtaaa agatgctttg cgtatggtcc agcttcctgg atacgaaaaa
    11641 cgggagatta gtgaaatgtc tggtggtcaa agacagcgtg tagcgattgc acgagcaatt
    11701 gtaaatgaac cgaaagtatt gctattagat gaaccgttat cggcgcttga tttgaaacta
    11761 cgtacagata tgcagtatga actgcgggat ctgcaacaac gattaggaat cacgtttatt
    11821 tttgttactc atgaccaaga agaagcgttg gcaatgagtg atgagatttt tgtcatgaat
    11881 aaagggaaaa tcgtccaaag tggtacaccc gtagatattt atgatgagcc aatcaatcat
    11941 ttcgttgctg attttgttgg ggagagcaat atcgtggacg gtgtaatgat cgaagataat
    12001 ctagttgagt ttgttggtaa gcaatttgaa tgtgtcgatg gcgggatgcg tctaaatgaa
    12061 ccagttgaag ttgttttacg cccagaggat ttgacgatca cgacaccaga taaaggaaaa
    12121 ctagtcgtaa cggtcgatac tcagctgttt cgtggtgtcc attatgaaat catctgttat
    12181 gatgaacagc aaaatgaatg gatggtccat tcaacgaaaa aagctaaaga aggaagcaaa
    12241 gtaggattgg cctttgagcc agaagatatc catgtcatgc gtttcaacga gtctgaagaa
    12301 gaattcgatg cacgtcttga cagctacgaa gaataggggg gacaagaatg aaaacaatgc
    12361 gcaggatcta ttccgtccct tatattttgt ggctcggttt attcgtgatc gcgcctgtat
    12421 taatgatcat ttatcaatct ttttttgata tgaatgggca attcacgctg aataattatc
    12481 tatcttattt tacgtcgggg acatcgcttt ctatgacgat caattctgta tggtatgcat
    12541 ttttgatcac gtttttcacg ctgatcatca gttacccgac tgcttatttt ttaacaaaac
    12601 tgaagcacaa gcaattatgg ctgatgctga ttattttgcc tacatgggtg aatctgctgc
    12661 ttaaagctta cgcatttatt gggattttca gtgtgcacgg cagcgtgaat catttctttg
    12721 attttatcgg gatcgggccg aaacagatat tattcacaga cttcagtttt ctgttcgtgg
    12781 caacgtatat cgagattcca tttatgatta tgccaatttt caatgcattg gaagagatga
    12841 atccttcttt gatcagtgct agtagagatt taggcgcgaa tagctttgag accttccgac
    12901 gagtgatttt tccgttatca ttaaatggtg tgaaaagtgg tgtccaagca gtatttattc
    12961 cttctctttc tttattcatg ctgactcgtc tgattggcgg aaatcgggtg atcactttag
    13021 gaacggctat cgaacagcat ttcttggtta cacaaaactg ggggatgggt tctacaatcg
    13081 gtgtgatttt aatcattgct atgttcatcg tgatgctcct gacaggcgag aagaaaaaag
    13141 gaggtcgctc ataaatgaaa aaatttaaat ggtcgtatct ttatttaggg atcgtctttt
    13201 tattcctata tgcaccgatc ttctatctga ttttttattc cttcaatgaa ggaggaacga
    13261 tgaacagctt tacaggattt acttgggaaa attatgaagc tgtttttgaa gatacacgtt
    13321 tgatcattat cgtattgaat acgttcatgc tggctttctt atctgcattg atcgccacga
    13381 tcatcgggac atttggggct atgggaatct attacacgaa aaaacgacgt gcacggacag
    13441 cgttgttgag catgaataac atcttattag tctctcctga tgttattatc ggtgctagtt
    13501 tcttgatttt ctttacacta ctaggattcg gattaggttt tcagagtgta ttgctttccc
    13561 atattgcttt cagcattcca atcgtcgtct tgatggtctt gccaaaattg caggaaatga
    13621 acgattcaat ggtcgatgcg gctagagacc ttggtgctaa taatttccaa gtagtcaaaa
    13681 atatcatttt gccttatctt tctccaggaa tcatagctgg atattttatg gcatttacct
    13741 attctcttga tgattttgcg gttacatttt tcgtcacagg aaatggcttt acgacgctct
    13801 cagtagagat ctattctcgt gcaagacaag gaatcagttt agaaatcaat gcgctaagtg
    13861 cgttagtttt cttgttctcc atgatattgg tcattggata ctactttatt agtaaagaaa
    13921 accgtccaaa acggctgaag aaaaacagaa gagtacgcga gggggtggca cggctgcgat
    13981 gaaaaaatta caatctctgt ttttaggtat tctcatcatt attgcggtgc tgctgcttgg
    14041 tgttcgtcaa cttgaaaaaa caagtggcat gagtggagca aaggtactta ccatctataa
    14101 ctggggagat tatatcgatc ctagtttgat cacgaaattt gaaaaagaat atggatacaa
    14161 agtgaattat gaaacatttg attctaatga agcgatgttc acaaaaatcc agcaaggtgg
    14221 gacaaactac gacatcgcta taccaagtga atatatgatc caaaaaatga tcaaagaaaa
    14281 actagtactt ccattagatc attcgaagat caaaggatta aataatattg atccacgatt
    14341 tttagacttg gattttgatc cagataatca gtattcgatt ccatattttt ggggaacttt
    14401 agggatcgta tataacgata aattctttga tgcaaatcaa attaagcatt gggatgattt
    14461 gtggaagccg gaattaaaag acagtttgat gctgattgac ggtgcacgag aagttatggg
    14521 gctttctttg aatagtttag gctattcttt gaatagtaag aatatggcac agctgacaga
    14581 agcagcaaat aaattgaatc atttaacacc aaatgtcaaa gcaatcgtgg cagatgagat
    14641 caaaatgtat atgatcaatg aagaagcctc cgtggcagtg acattttctg gcgaagctgc
    14701 cgatatgatg tgggaaaatg aacatttaca ttatgtgatc ccaccagaag gatccaatct
    14761 ttggtttgat aatatcgtta tgccaaaaac cgtgaaaaat aaagaaggtg cctatgattt
    14821 catcaacttt atgctagaac cggaaaatgc ggcacaaaat gcagagtata ttggttattc
    14881 tacaccaaat gaagaagcaa aagcgttatt gccaaaagac atttcgtcgg acgaacaatt
    14941 ctatccatct gatgacacga tctcccatct tgaagtctac aaagatctag gatcgaaata
    15001 cctaggcatc tataatgacc tattcttaga attcaagatg tataggagat aatggcaaaa
    15061 ctcagtaaga atggggactt atcaacgttc taaagagcta aaaatcgtcc tttttctatc
    15121 tgagttttcc aatcttctac caaaagcgga tgaaacgccg ctttttttgt gttattttat
    15181 tttttctacc acgtttttct accagagaaa agtggacaaa accattgtaa aatagggccc
    15241 gagacgggct agtaaatcta agattgaaaa agttacagaa ggataaagta attggaaact
    15301 atgcaaatat acttaaccct tctgtaatct attaataaaa ctaatatatg aatccagttc
    15361 aatatttgta gctatgaaaa aacataaaaa tcatattaga taaaaactaa attggtagat
    15421 aaattatttt gtcagatatc tgaggcaaat attcgactat ttgtgtttca aagtaaaaat
    15481 tggaatacaa atatagaata gacatattgc agccgatagt ttattatgct tttagacaca
    15541 ttaaactgga ggaaaaaatg aaaaagagcg ctaaagatcg agtttttgat gagctgatac
    15601 gactaacagg gaatatgtct tctgtcactg ccgagaaaat ttcgaagaat ctaggtatct
    15661 ctcgtcagaa tgccagccat tatttgacga gattagtgga agataaaaag gtcgagaaat
    15721 taccgggaaa gcctgttttg tggaagcctt tagatgaata tgcggttgta gataatacgg
    15781 agcaaataaa cgaagccttt cattctgtag ttggtcatga cggaagtctg agagaggtca
    15841 ttcaaaaatg tatagccgct gtcaaatatc cgcctaacgg actcagtgta ttaataaatg
    15901 gagccacggg tgttggaaaa agttttttag ccacaaaaat ttttgaatac gctatccatg
    15961 agcaaattat tgaaaaagat gcaccatttg ctatcttaaa ctgtgccgat tatgcggaca
    16021 atcccgaact gctttcggca acactgtttg gatacaagaa gggggccttt acgggggctg
    16081 aaaaggatac cgatggttta ttggcaacgg ccaacaacgg ctatcttttt ctcgatgagg
    16141 tccatcgcct atcgaaagaa aatcaagaaa agctctttct atttattgat actggaaact
    16201 atcgtccagt cggtgaaaat gtaaattggc attctgctaa ggttcgtttt attttcgcca
    16261 cgacggaaaa aggtgaaaat tatttattag acacctttga tcggcgtatt caaattagtg
    16321 ttatgttgcc aacttttgaa gatcgcccca ttcgagaacg attagagctg attcaattat
    16381 tttttcaaaa tgaagcggat gttttacaaa aagatattat tgtttcaaga gaagccttgt
    16441 ctttaatgtt atctcatccc tttagtggga atattggtaa gctgaagaat atcattaaaa
    16501 tcagttgtgc ggatgtgtat agtcgaacac aagaagaacc attaacgatt gggaaaaatg
    16561 agatcttgat tcagttaaat atgctggaat ccgaagtaga aagcttgccg attgctagtt
    16621 tattgataaa gaaaaatcaa aagataaaaa cggatcgaat caaaccgttt gattatgatt
    16681 cgattttgga aaaaattact cagctgataa atgaaggtaa tttcaatgaa aatttttctt
    16741 cgataaagaa tgaagtacag catttggtgc atcacattga taaggattct tttttatcaa
    16801 tgagtagaaa attgatgatt cagatgttta gaaaggtctg ggtatccgtt ttaggaaaaa
    16861 cgtacggatt gacgacgagt ggacctgttg cagaaattat tagcagatcc tatgtcattt
    16921 ccccaagcta taagacagac ttaaccggga taaatcgtac attagctgca aatctacctc
    16981 ggacgactta cttatgtaat cagttcttgg agcatttacc accgctctca gaaaaggacg
    17041 agaactttct aagactgctt attgtcgtga ctttatctga ttatgtagat gaatccatcg
    17101 agctgaaggg cctgttgctt gctcacggag atagtacggc tagcagcatt caagcagtag
    17161 tgaatcaatt atgcggcaat tatgtttttg aagcgttgga tatgccagta gagagtaatg
    17221 tgtcagagat tgtcgaatcg acaaggaatt tcgtgaaacg gcaagtgaat acagatagtc
    17281 tagtgctgat cgtagatatg ggctctttaa atcaaattta cacacagatc aagaacgatt
    17341 taaaagggga gttacttctt ctgaataatt tgacgacttc cattgcactt gatattggct
    17401 tgaagatgac gatgaatgcg ccatttaaac agattgcgga ggatgcagca gacaagtatt
    17461 caatcaatgt tcaatacttt gaaggctttt ctcaaagtag caacattatc atttcctgta
    17521 tgtcgggttt ggggatttcg gacaaattga aagatgtttt tcgtcatcat ttatcggatg
    17581 aatccatcga agttttcacg aaggactaca gagaactacg tgaattaatc gaccaaaatg
    17641 atgaaactta ttttgacaag acaaaattgg tcatcacgac ttcggatctt ccacgttcct
    17701 tttcaattcc taatattaat gtttatgaca tcttagacgg tgatggagca gccaatttgg
    17761 agcatatttt gagtcctgaa atgaagccgg cagaatttaa ccgcttgatt caggagctag
    17821 taaaattctt ttcgttggaa ggaattgcgg accgtttgaa ctttctgaac ccaacgatcg
    17881 tgatgagtga agtggaaaca gtcatttcta aatatgaaaa ttactatcat ttcactttaa
    17941 atggccgtgt aaaactaaat ctctatatgc atacggcatt gatgatggaa cgtttattct
    18001 tggctcggac agaaaaatct gaagaaccga attatgattt gaaaccagat gaagatgaat
    18061 tttattatgt ttctagaagt atttttcaat caatggagat gaaatataat tttaaagtca
    18121 atgcttatga gctttcgctg ctctatgaat tattaggacc gtatattcaa aaataaagtg
    18181 tctaaaaaag tgtctaatct gtttttttta ggttagacac tttttttaat gtgaattaac
    18241 gtgattaaac caatgttttt aaaagttggc acaggaattg cttatataaa agtgaacaga
    18301 aagaaaggaa ttaaccatat gaagaaaatt ctaatagcga ctcacggtca cttagcttca
    18361 ggctttctta gcagtattca attactgacg gggaaggttc aagagattac ggtgattaat
    18421 gcttatgtgg atgagtgtga ttttgaacag gagttgaatg tatttgtaaa ccaggtgaac
    18481 gagcaggatc agatttttgt ttttaccgat ttatttggcg gaagtgtcaa tcagaagatt
    18541 accaaaacct ttttggagaa agagattgct gtaactattt tgtctggctt taatctgcca
    18601 attctgctcg aaatagtatt ggcaaccaag gaattaacaa aggaggatat tcaggaactg
    18661 gtaaataaat gccaacagga attaaagatg accattcttg atgaggtaca tgtagtaaat
    18721 gaagaggaag atttttttta ggaggaagaa aaatgattaa agcagtgcga attgatgaac
    18781 gattaattca tggacaagta gcgatgactt ggacaaagag tctaaattta accgggttag
    18841 tagtagcaag cgatgaggcg gctaccaacg atattcaaaa gatgacattg aagatggctg
    18901 caccatcgaa tgtcaaagtc attattaaaa cagttgatga cgcgattcat ttgttaaaag
    18961 atccgcgggc gaaggacatg cgcttgatgg ttttagtccc taatgtcaaa gatgcggtaa
    19021 aagttgcgaa agctttccca acggaaatcg aattaatgaa cgtggggaat gctgggaaaa
    19081 tgacaccgcc atcggaagat aagaaaacct tatcaaagga agtgatgtta acgacgtcag
    19141 aaattcaaga cttaaaagaa ttagttgaac tttatccaga taccgttttt cagcctacac
    19201 cggcgatgga aaaacgttca gctatctcta ttttgagtga tttttagaaa aaggaaggag
    19261 aaaaattatg tatgatgcat ttgtagtcgc tttggccgtt tttattggag ttgctggaca
    19321 tgaaatgttc gggatgtcca tgctgagtcg tccgattgtt gttgctccgt tagcaggagc
    19381 gttgatgggg gatttgcaga cggggttgat tgtcggggcg tcactagaag ccatctttat
    19441 gggtgtcgtc aatatcggaa tctctagtac ggcggagcct tctttggctg ctgggctggc
    19501 tacagcgttc gcaatcaaaa tgggaggaaa cgtggatgcg attattccca ttgtctttcc
    19561 actagcagta ttaggattac agttaatgaa tatgattttt tcttttgtat gtggaccgat
    19621 ggctgctaaa tttttcaaat ttgcaaaatc tggtgatcaa aaaggaatca tcaatctgca
    19681 cttctttatg tggtttgtcc actatgcgct ttatgctctt attccattct ttgccgtttt
    19741 atttggagca gatgttgtca aaagcgtctt ggataatatt ccagaggtca tcatgaacgg
    19801 attaacagtc gctggaaatc tgctgccggc agttgggatg gcgatgctgt tacggatgct
    19861 ttggagtaaa aatattgctg tgtggttttt ctttggggcc gttgttatgg cgtatctcga
    19921 gttaccattg attgcaattg ccgcagttgg aacgattatt gcaattgtta tagcccaaag
    19981 agatctacaa ctaaaaaata tatctgttca tgcagttcaa agcgatgctg ctgtaaatgc
    20041 aacgcttgac caagaagagg aggatttctt tcaatgaaat tagctgatgg aatttctaaa
    20101 gatgagaaaa aattggttcg gaagatgttt tggcgctcga tgactatgta tgcttcggtg
    20161 aatccggtga cgatgggcgg cggtggtttc gcctattcta tgatgccctt cattaatcat
    20221 ttctacaaaa gtgaagaaga tcgagctgcg gcgctggaac gtcatacaca gtatttttcg
    20281 acgaccattc cttgcgcgtc ctttgtcatg gggattgcgg catcgatgga gaaggaaaat
    20341 tctgaaaaag ctggttttga cgtggaaagt atcaacgcga tcaaaacgag tttgatggga
    20401 ccattggctg gaattggcga ttcattgttc tggggagttt ggcgtgtcgt taccgcggga
    20461 ctagcgatca acttagccaa tgccggaaat attttggcac caattatctt tttagttatg
    20521 ttcaatatac ctaattactt gatgcgatac tatggcggcg ttttaggcta ttcattaggc
    20581 tctaaatata ttgaaaagct gtatgcgaac gggttaattg aaattctgac aaaagctgct
    20641 agtatcttgg gtctgatcat ggtaggtgca atgacgatca gtatggtcaa attcaatacc
    20701 gttttgagct tttcaatgaa gggcgaagaa ctaatgaatg tccaagaaat cctcgatagt
    20761 atttttaaag gaattgtacc gttattggta acattgagct gtttcaaatt actagataaa
    20821 aaagtcggcg tcatcaaaat tatttttgga atggtcgtct tgggaatttt attgtcatta
    20881 cttggaatcg catagtagga agctgcagct actaggctgc agttttttta gcaaggaggg
    20941 aaaagatgac tattaatgaa gtaatttatt cacccaaaca cccatttttt aatgctgagt
    21001 taaacgacgg aaatgtgact acttcaaacg aatacagcta ctatcggaat attcctgatg
    21061 taaagggatt gaccttcgat gtaagtaatc aaaagacatt agcaaacctt gatgtttgcg
    21121 ggacgattca aacgttgacc ttttatcgag aaaatttact aactgaggaa aaaccaggtg
    21181 tttgggtcaa taaacagctc tctcaaacca attcgttatc cttaaaggtt gaagtaaacg
    21241 ggattgtcta ccatttgtca gaatcggatc atcgtattga agtcgatgtg attcaagact
    21301 gtttgcctag atatattcat cattattcga cgtttaaggt cattgtagta tcctttgctc
    21361 ccatactggc taataagcgt ttaagtatgc ttgttcaaca ggtttacatt gtgaatgaaa
    21421 cacacgaaag ccttcaagtt tcagtgaaag atgtgccttc gtatcaagag aagtattcgg
    21481 atcagcaaaa tgtgttgatt tctcaaaaag gaaataagga cacgatcgca caacaggaag
    21541 tggcaagctt cgccattgct tttgtggacc caaatgcttt tgaagaagta atgacttttc
    21601 aggaatctga tatcgaaaaa tggctgaagg aaacattgaa ctattttgct cagctatttg
    21661 ggcagctgtc aatgccaaac gatagagttg tccatctcta tcgtcgtgca ctttatcaaa
    21721 gcttttctag tttcggtatg aatcgacagg gacaggtcgt cggcagcaat tggggcagct
    21781 atcctgctac taatcgaatt tggaataagg atatgtatta cgcatcattg ccattcacca
    21841 tgtttgaacc ggagttatgt caaaaaacgg ttctatggtt tgatcaatat ggcgttaagt
    21901 ttcctggttc aaaatttaca ggaggtatca atcattctct gagtaattcg ttatccagtg
    21961 cgttactggc tagtctttat tttgaacata ctggggatac aaaattcttt gagcggtgtc
    22021 cgcaggttct actgaatgct tcaagaatta ttgaggacat attagctcaa cgtcggtcag
    22081 gggaaccaat gctttttcca tctgtttggt tatcagacgc ctttgcactt ggaaagtatc
    22141 ataccggcag caatctttgt ctttggaagg cctgtattgg acttgcagaa atttttgaag
    22201 ccctagaaca actgagtctt agcaagcgat acaaaaacat tgcctgtaaa ctaaaggaat
    22261 caatttgtaa acaaatgact atcgatggtc cttttggtga gcaatttttg gagggaatcg
    22321 gtgatgagga gaaaacaacc tattcagtga agcactatca aaaaccgatt cttgagcaag
    22381 ggttaatctt tttaagtgat gtgattgtgg atggaaaaat taatttattg atgcatgatg
    22441 gagaagaatc agacactacg ttgatgcctt tttatcaatt tttagataat aaagatcatt
    22501 tatatcagaa caccatggcc ttttctgcta gttctttcaa tccaacctat agcgaggaaa
    22561 tcaaaggcat cacttgggga ttagaatccg gcgccacttt tcctggcttt atcactgttt
    22621 taatgagcga gctgcacaac cctcaagctt tcgtaacccg attagaagaa ctaatttgtt
    22681 tggcagattt agacggttcg tggtggtggt ggccgtatcg cttagaggct caacaaggag
    22741 aagtcgtgcg agactttggc tgcggcaaat gcggctgggc atcgggtctt tttgtcagcc
    22801 taatgatcag tcaatatttc ggattgaaat tcaaaaaagg agtattgcaa attgatcctg
    22861 ttgataattt aacgttttct tggaagaatt tgaagttcgg tcaagccttc atttcgattg
    22921 aatgtacgca atccgagttg agtatttcaa atttgtcaga aaagccattg aaaataagtg
    22981 atacgaaaaa aattcttcac gtagaaaagg gaaaaacgat tcacattcaa aggagaaaaa
    23041 gatgagtata ctaaaaaata ttacccaatt taaaaaaatc cagagtcgag caatcactcc
    23101 cgaaaatcca acaggcgaga aaggtaaagc tgcgatgttg ccaagcgagc taggaccaag
    23161 ccgcaaaggt cgtggcgcaa ttccgctgac ttctggtgaa gaaactgtga ttgcggatat
    23221 tactggaacg ggaataattc gtcatatgtg gatgacgatt cgtgatcgaa cagaatttgg
    23281 cagttttgtt ttacgtgatt taatcttaag aatttattgg gatgggtcgg atagccctgc
    23341 tgttgaaagt cctttagggg atttcttttg taatgggttt ggagagcgtt gcgaggtgaa
    23401 ctcgttgccg atagtggtta atcctacagg gggctttaat tcctatttcg aaatgccttt
    23461 ccaaacatcc gctaaaatta cgatcgagaa tttacatccg aaggatatgt cctcattttt
    23521 ttatacgata aattacgctt tggtggacga attggaagaa aatagcctat attttcacgc
    23581 aaattggcat cgggaacgca ttaccaaatt acaggaggat tataagttaa ttgatcgatt
    23641 aaagggtcaa ggctattacg tgggtactta ccttgcgctt acctgcctag aacgttattg
    23701 gtggggcgaa ggcgagttta aattctacat tgatggggat agtgagtatc cgaccgtaac
    23761 ctcaacaggt tcagaagatt attttggcgg ggcgtgggcc tttcatgagt acgatgagtt
    23821 cggacgtcca catgcgaaaa actattcaac agccttttta gggtatcctt accaatcaaa
    23881 tagagatgcc acaagagatc gttttgaaac gggaaaattg aatgccgttc atgcctttgg
    23941 tgacgacgca atgccacgac atggtcttta tcgttggcat cttcaagacc ccatctgttt
    24001 taccgaagat ttatcggtca ctttacaaca aatcggcaac gatgatgttc gcttatttga
    24061 gcggcaagac gatgttgcta gtgtcgctta ctggtattca acagaagctt caggaaatac
    24121 taaacgtcat ttcactgatc gagaacgctt gccgcgttaa tgaagtgttt ttcacatatt
    24181 cgaagggagg aacctatgat gatatatggg gcaattgaag ctggagggac taaatttgtc
    24241 tgtgcagtcg gtactgagga attaacgatt ttggaaagag tcagttttcc cacaacaact
    24301 ccagttgaaa caatgaagca ggtcattgat ttttttaaac cttttaagga acaattggca
    24361 ggcatcgggg tcggctgctt tggtccaatt gatattcaat gcaaatcaac gagtttcggt
    24421 catatcacat caacaccaaa acttgcttgg caaaatttca attttgtagg aacgctaaag
    24481 caatatttca atattccgat tgaatggaca acagatgtca atgcggcttg ctatggtgaa
    24541 tatgttgctg gttctggcaa agggttagca ggtgttgcat acttcactgt gggaacaggt
    24601 atcggaggtg gtgccttgtt caatgggact ttcgtggaag ggttcagtca tccggaaatg
    24661 gggcatatgt tgatcaagcg acatccaaag gacaattttt caggaaactg tcccttccac
    24721 caagattgtt tagaggggat ggcagcagga ccagcgattg aaaaacggtt gggtgtaaaa
    24781 ggccagaatt tattggctga cgattcgttt tggcagattg aagcctttta ccttgcccaa
    24841 tgtgcataca atacaacact gatgttttcg ccagatagaa ttatttttgg cggtggtgta
    24901 atgaaacaag aacacatgaa gaaaaaagtt caggataaat ttgttgagct tataaatgga
    24961 tatgttgaaa taccaccaat tgatagctac attattacgc ccgaattagg tgataatgcc
    25021 ggaataatcg gcggattagc attggccaga aaagcggtaa gaaataagca accataatta
    25081 atgaatgttt cattgatact catgtcaaaa gtatttaacc gagtaatcaa atgatcaaat
    25141 gaagttggat aaagcgaaaa tgaattcaaa taaaaaggct ctaagtcaaa tcgtgttgtt
    25201 ttcattaaat cactgaaagg aaatgcttct gcgtttcctt ttttcttttt gttgtgcgct
    25261 gtataccttg aggatattca ctggtggcgt acacttaaat agtaatatat tgtgttactt
    25321 tatgtctgtg gtaatagtta tagttgctta ccaatgtatg ttcatttttc gaaatgttga
    25381 cttcataatc cttggcttaa tgtttgaatt tttttggtga tagatttttt cagccgtata
    25441 aaataaatcg taattctatt atagagagtt gtgattataa aaagatagca agaaaaaaaa
    25501 aga
//
LOCUS       DACCYH010000036        24259 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.036, whole genome shotgun sequence.
ACCESSION   DACCYH010000036 DACCYH010000000
VERSION     DACCYH010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.036"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(44..1339)
                     /gene="purB"
                     /locus_tag="IVY85_001410"
     CDS             complement(44..1339)
                     /gene="purB"
                     /locus_tag="IVY85_001410"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP8044435.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1352..2485)
                     /gene="purK"
                     /locus_tag="IVY85_001411"
     CDS             complement(1352..2485)
                     /gene="purK"
                     /locus_tag="IVY85_001411"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP8044436.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2469..2957)
                     /gene="purE"
                     /locus_tag="IVY85_001412"
     CDS             complement(2469..2957)
                     /gene="purE"
                     /locus_tag="IVY85_001412"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP8044437.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3373..4680)
                     /locus_tag="IVY85_001413"
     CDS             complement(3373..4680)
                     /locus_tag="IVY85_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP8044438.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4683..5264)
                     /locus_tag="IVY85_001414"
     CDS             complement(4683..5264)
                     /locus_tag="IVY85_001414"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP8044439.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5380..5477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5836..7128)
                     /locus_tag="IVY85_001415"
     CDS             complement(5836..7128)
                     /locus_tag="IVY85_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8044440.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7125..7748)
                     /locus_tag="IVY85_001416"
     CDS             complement(7125..7748)
                     /locus_tag="IVY85_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044441.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7751..8587)
                     /locus_tag="IVY85_001417"
     CDS             complement(7751..8587)
                     /locus_tag="IVY85_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8044442.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8604..9491)
                     /locus_tag="IVY85_001418"
     CDS             complement(8604..9491)
                     /locus_tag="IVY85_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8044443.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9507..10790)
                     /locus_tag="IVY85_001419"
     CDS             complement(9507..10790)
                     /locus_tag="IVY85_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8044444.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11473..12156)
                     /locus_tag="IVY85_001420"
     CDS             complement(11473..12156)
                     /locus_tag="IVY85_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP8044445.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12174..12791)
                     /locus_tag="IVY85_001421"
     CDS             complement(12174..12791)
                     /locus_tag="IVY85_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP8044446.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12801..13625)
                     /locus_tag="IVY85_001422"
     CDS             complement(12801..13625)
                     /locus_tag="IVY85_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP8044447.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13618..14178)
                     /locus_tag="IVY85_001423"
     CDS             complement(13618..14178)
                     /locus_tag="IVY85_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044448.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14352..15308
                     /locus_tag="IVY85_001424"
     CDS             14352..15308
                     /locus_tag="IVY85_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8044449.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15613..16569)
                     /locus_tag="IVY85_001425"
     CDS             complement(15613..16569)
                     /locus_tag="IVY85_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP8044450.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16673..17275
                     /locus_tag="IVY85_001426"
     CDS             16673..17275
                     /locus_tag="IVY85_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8044451.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17363..18862)
                     /locus_tag="IVY85_001427"
     CDS             complement(17363..18862)
                     /locus_tag="IVY85_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP8044452.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18878..19402)
                     /locus_tag="IVY85_001428"
     CDS             complement(18878..19402)
                     /locus_tag="IVY85_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044453.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19700..20449
                     /locus_tag="IVY85_001429"
     CDS             19700..20449
                     /locus_tag="IVY85_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP8044454.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20440..21225
                     /locus_tag="IVY85_001430"
     CDS             20440..21225
                     /locus_tag="IVY85_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8044455.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21194..21910
                     /locus_tag="IVY85_001431"
     CDS             21194..21910
                     /locus_tag="IVY85_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8044456.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22012..23031)
                     /locus_tag="IVY85_001432"
     CDS             complement(22012..23031)
                     /locus_tag="IVY85_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP8044457.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23050..24108)
                     /locus_tag="IVY85_001433"
     CDS             complement(23050..24108)
                     /locus_tag="IVY85_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP8044458.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         7188 a         5222 c         4225 g         7624 t
ORIGIN      
        1 ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc aaacctactc
       61 gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca tcgtctattt
      121 cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg cggaactgca
      181 cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct tcttctcttg
      241 tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg
      301 cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg atattgccaa
      361 aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt cgttctgctg
      421 aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca gtgaccatgt
      481 gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga ttccttttgt
      541 gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt
      601 cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta gcaataagcg
      661 ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta gagatttctt
      721 gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg atatttgcga
      781 acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca tgttcaaatc
      841 gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca aaagtagttg
      901 gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt tcttttgctt
      961 tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca tttgcttgct
     1021 tgatgagata gccataagca gtatctacga catccgtact tgttagacca taatggaccc
     1081 attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc
     1141 gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc tcacgaattt
     1201 ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct gctaaaatct
     1261 cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc atttcagggc
     1321 gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt tgtttgattt
     1381 cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc ttgcgacctt
     1441 tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc tcaatcaagc
     1501 gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg cttatcaaac
     1561 gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg aactgactca
     1621 tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct aattcattca
     1681 catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct gaaaggctca
     1741 ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc tgatcaatcg
     1801 cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc acgaccggaa
     1861 aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct ttttcaaacg
     1921 ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca ggagctagat
     1981 ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga gtggttttca
     2041 aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga ctgacgatcg
     2101 ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct aacaggcggt
     2161 cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt aaaatcgcat
     2221 ttaacgcctc tacactgacg ttttcgaatt cataggtaat gacatctgtt cttctggcaa
     2281 gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct gcaacttgtg
     2341 cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt tcttttgcac
     2401 taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt gaaccaggca
     2461 acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa cattgaacgt
     2521 ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg tatcgcaagt
     2581 agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg tactcctcca
     2641 ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga ttggaccggt
     2701 acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg cgcggctccc
     2761 cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc atattcaaac
     2821 atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac accgaattca
     2881 tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc cataatcaca
     2941 gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt ttaacaatca
     3001 ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt attgttcgtg
     3061 tttattaaag ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct ttttatggat
     3121 aaacggtgtt ctcctgccaa ctcctcggtc acaaggaaca atattcaaca aacatgagaa
     3181 gctttttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttc
     3241 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
     3301 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
     3361 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
     3421 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
     3481 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
     3541 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
     3601 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
     3661 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
     3721 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
     3781 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
     3841 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
     3901 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
     3961 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
     4021 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
     4081 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
     4141 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
     4201 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     4261 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     4321 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     4381 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     4441 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     4501 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     4561 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     4621 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     4681 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     4741 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     4801 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     4861 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     4921 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     4981 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     5041 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     5101 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     5161 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     5221 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     5281 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     5341 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     5401 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     5461 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     5521 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     5581 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     5641 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     5701 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     5761 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     5821 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     5881 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     5941 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     6001 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     6061 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     6121 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     6181 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     6241 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     6301 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     6361 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     6421 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     6481 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     6541 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     6601 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     6661 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     6721 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     6781 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     6841 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     6901 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     6961 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     7021 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     7081 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     7141 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     7201 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     7261 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     7321 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     7381 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     7441 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     7501 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     7561 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     7621 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     7681 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     7741 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     7801 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     7861 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     7921 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     7981 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     8041 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     8101 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     8161 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     8221 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     8281 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     8341 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     8401 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     8461 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     8521 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     8581 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     8641 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     8701 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     8761 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     8821 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     8881 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     8941 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     9001 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     9061 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     9121 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     9181 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     9241 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     9301 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     9361 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     9421 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     9481 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     9541 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     9601 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     9661 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     9721 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     9781 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     9841 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     9901 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     9961 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
    10021 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
    10081 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
    10141 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
    10201 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
    10261 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
    10321 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
    10381 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
    10441 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
    10501 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
    10561 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
    10621 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
    10681 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
    10741 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
    10801 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
    10861 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
    10921 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
    10981 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
    11041 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
    11101 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
    11161 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
    11221 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
    11281 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
    11341 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
    11401 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
    11461 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
    11521 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
    11581 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
    11641 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
    11701 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
    11761 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
    11821 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
    11881 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
    11941 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
    12001 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
    12061 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
    12121 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
    12181 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
    12241 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
    12301 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
    12361 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
    12421 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
    12481 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
    12541 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
    12601 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
    12661 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
    12721 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
    12781 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
    12841 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
    12901 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
    12961 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
    13021 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
    13081 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
    13141 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
    13201 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    13261 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    13321 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    13381 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    13441 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    13501 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    13561 aaaataacat agcccattgc tgcgatcata atcacaactg ctccaaatcc taaaaattta
    13621 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    13681 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    13741 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    13801 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    13861 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    13921 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    13981 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    14041 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    14101 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    14161 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    14221 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    14281 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    14341 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    14401 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    14461 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    14521 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    14581 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    14641 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    14701 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    14761 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    14821 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    14881 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    14941 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    15001 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    15061 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    15121 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    15181 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    15241 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    15301 atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    15361 acaaaaatag ctcctcctat ttttcgtttg gctggactta ttaccgcaga ggaaagcttt
    15421 tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca gctccgagtc acaaggagca
    15481 atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttgac
    15541 aggagaacac cgtttaaaga agaggttgtg aaatcagctt tttgacctga atattagctg
    15601 aattcacaac ctctattttt ggattttttg ttgggagaat tctgccttga tttcaataaa
    15661 acttttaaaa gactgcaaaa actggaacaa ttctgctcga tcctcgaatt cagggcgact
    15721 agtcggcaga ggcatttgcc gatacaatac ataaacttct tcaatacgtg ttaaaatttg
    15781 tctgccatca tttgactctg aaaatgtttc tgcagtataa atcaataaac catagatatg
    15841 ttttccataa taagctggtt gctggattct ctctagattt tccagcatgt ctttaatcac
    15901 attggcctgt gctcttctca tagagaagta agtttcgtag tatagcggct gtctgagcca
    15961 gtagttttct tgatgttctc tggcatcttc ttggctttcg cgaatgaatg tcagtaaatg
    16021 ttcgcatttg atctgcacat cttctttatt ttctgataaa agaaattctg ccatttgtct
    16081 caaaatattt cgaaattctt tttcgatctg tatttggttt tccttcagct gcttcgtacg
    16141 atctggcatg taaacatttg caagcaaagc aaatactaat ccaatcgcca ttaatcccgc
    16201 ttcattgccg atcagctgcc aagacatgtt ttcctccact aaataatgag tgaccaaaac
    16261 cgaatttact actatccctt cggttaattt gaagcgagag gcaagcggga tgaacaggaa
    16321 taaaaaaaga ctgaagccaa aaatagtata cccaacagca aaaaaactga acagtgctgt
    16381 tgccgtagct aatataaagg ctgcgattcg attcatgccc gtacgcaatg tagagcgctt
    16441 ggtatttcct acacttaata atgcaatgat ccctgcagct ggccaataca acagcgataa
    16501 gctacccgct gtgaccatcg aaaagaagac actgatcact gttttgactg ttcgcattcc
    16561 tatacgcatt tacttccccc ttttttattt taattatact tgaataatga aaaactagct
    16621 atcagacaaa agtttaggta tgatagtaaa aagaccaaaa aggagaatct ctatgcaatt
    16681 tggccgtttt cgactaggta tgcgtacgat caaaagtgcc ttagctgttt ttttatgtat
    16741 cttgtttttt catttgactc aacgaggaat cccgatgatt gctgcattat ctgctgtttt
    16801 ttctttgcgt caggacttga ctacaaccgt ttcttttgga aagtctagga tcatcggcaa
    16861 tacattaggt ggtacattag ctatcattta tttttttgtc aaaagctatt ttcacaatga
    16921 ttttttggta gaattgatct tgcttcctat tctagttatt attgtgatcg tcgtgtcaga
    16981 tggtattaac aataattccg gtatcatttc cgcaatagct acgttgttgc tgattgcgtt
    17041 gagcatccct caaggcgaat ccgctttgta tgctatccaa cgtgtactag atacttttat
    17101 agggacattc attgcaatcg gtatcaattt ctttcttcgt cctcctgaaa cagaaaagaa
    17161 ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa gaacgattgc taagaacaaa
    17221 tttacaagag atagaagcta agatccaaaa acaaaagaaa aatagcgagg actaattttc
    17281 ttttgcaata aaataaaaaa atggagagaa gaactttaaa aagtcttcct ccatttttga
    17341 ttgaacggtg aagtgtacta ctttattctt ccttcatcga gttttgttct gcctcttttg
    17401 gcgcttcata catatttact tggctagtag tcccacgtgt cgacaaattc tctacgattt
    17461 ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg agtagaagaa agcaaatttg
    17521 tggcataatt tgtcactcgg ttcgctccac tggtttctcc tgaactgctt cctgtatcga
    17581 caacagagat tttttcgata tttcctagcg gctgtgctgc ttcgcgcatc aattgcggta
    17641 gcatatcgat aaccatactc aagacagctg cttcgccata ttccttgaag gcattggcga
    17701 tcttttgttt tgcttcggct tctgcttttc cttttgctag tgctgcttcg gcttctgctt
    17761 gtccagctaa acgtgtctta ttggcttccg cttctgccag tgcttccaca cggaatcgtt
    17821 ctgcttcggc ttcagtcact tctttgactt tttgagcttg tgcttcttgt tctttagcgt
    17881 accgatcggc atctgctttt ttcttcactt ccgaatcata ttgcttttca cgacgtgtga
    17941 tttctttttc ttccagttcg atttgttttt gacgttctac gattttaatc tgcatttcct
    18001 gttcgattac ttgttgttgt gctcgggcac tttctaaatt gtaggcttgg tctgcttttg
    18061 ctttagcaac atcttgttct tgtttgtagg ttgctaattt cagttctttt tcttttaatg
    18121 attctgcgat ctctgtttgt cgctgtaatt cagctgcttg tgattctttt tctgcttgag
    18181 cttttttgat acgcgtttct tttagagctt ccgcttctgc tatatctgca tctcttttga
    18241 cttgggcgat tcttggtttc cctaaagagt caagataacc gtttttgtct ctgacctctt
    18301 tgatagtgaa agaaacgatg atcaacccca tttttgcaag gtctacagaa gcgacttctt
    18361 gtacgctttg actgaactta tcgcgatttt gatagatttc ttctacagtc atagaaccaa
    18421 gaatcgagcg caagtgtcct tctaatactt ctcttgcttc attttccagt tcttctcttg
    18481 tcttgcctaa aaactgttct gctgctgtag caatctcttc tacagatgaa ccgattttga
    18541 tgatcgaagt accatcacac atcaccggta cgccttgttc cgtatacact tcaggggtgg
    18601 agacgtccaa tttactcgat aatagactga tccgattcga acgttgaaaa actggcaaga
    18661 cgaatgcgcc acctccacgc acaattttga ttttgttatt gctttcatca atgtgcacat
    18721 ttttgcttcc taaataactt ccactgatga tcaacgcttc atccggtttt gctgtctggt
    18781 acttcgctac aaagacgatc agcaacatca gtaaaatcaa agctgcgatt gcgatcgcga
    18841 taaaaacaac tggtatttcc atttaacaca cgctccttta agcttttatc atgctttcat
    18901 aaggcatgac ataacagatt ctttccttga tctcaatgat caatactttc gttccccgct
    18961 cagctgtctg ttcttggggg ctgtaaaaac aagctggacg actaatggaa ccggtaacac
    19021 ttttcaactg aatttctccc atacctcgta cagggatcgg tgtgatcaca acagctactt
    19081 gtccttccag agttttttca gaagacgaca tcgttgattc ggcatgtttc atcggcatca
    19141 atatatagaa attaagcaaa aataccaaca ctatcgatat gcctccacta gcaataaata
    19201 aaaataaaga tgattgatct gttaaacgct cacctagaaa accaaataag cttgtgaaag
    19261 ttaaccaagg gataataagc atcggatcaa gcgggccatc aaaatcgaat acatctccaa
    19321 aaataatcaa taggactgcc aatcctgcac aaatcagcaa tgtataccaa taaatcgtct
    19381 caagtgcgat tccccctatc atatacacgc ctccttccat ttctacgtct atcataccaa
    19441 tgtttgaaaa ttctgtatag cttcttttac cgactcagtg gtgtttttta taggaaggtt
    19501 gtgtttatcg ttgacaattt gttaaatttt tgttttcatg ttaaaatttt cacaataaat
    19561 tctttcgagg tagcctatgt ttcctttttt taagaaaaaa ccattaaagc tccagaacac
    19621 gatcatcctt gtcgtttgtt tctccacatt cgtttctttg attgtttctg ccatattgat
    19681 caggaatttt attctttcga tgaaccgatc tagtgtgtca gataaaatct ctagcattgc
    19741 taaaatcgtt gccaatgata aactcatcca aaaaaagctg cagactggtg acccgacaca
    19801 tgcgattcaa ggaataacaa atgaaatcat gattgcatcg aatttagatt ttatcgttgt
    19861 catggaccct gaaggcattc gatattctca cgcagaccca agttacatcg gtcttccttt
    19921 ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa gaatttttct ctgaaaaaga
    19981 aggaatgtta gggttcggct tccgctattt cgtccctatt tattatgatc aagagattat
    20041 tggtgcagtt tgtgttggct taacaaacaa cacgattgac aatcaagtca aagatggaca
    20101 aaaaatgatt gttttttcat tgattattgg acttagcgta ggcatcatag gcgccgtttt
    20161 tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa ccacaagaaa tagcagaaca
    20221 gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa ggaatcttag ccatctcttc
    20281 tgataaaact atcctcttga tcaatacacc tgcaaaaaac attttctacc aaacagagca
    20341 gctattggat ttcgaagtag gaaaaaaatc agtgataatc tatatgacgt tttgtttaaa
    20401 gagacgattg agcatcaaac gaccgttcgg actcaatcca tgtatttaaa tgggatagaa
    20461 actatttcta gcgttacacc tctttttagc aatgaccgtt ttttaggagc agtcgcaact
    20521 ttacgaaatc gatcagaagt aatgaaatta atggaggagc ttggggggac gaaacaatat
    20581 ataaactcca tgagagctca aacccatgaa ttcatgaata aacttcatgt catttctggt
    20641 ttgattgatt tgaagaagta tgaagaagtc acccatttta tccagcagct aaatcaaaat
    20701 tataaaaaag agattggcca agtctcaagt ctgatcaaag tacctgcaat tgccggtttt
    20761 ttattaggca aaataaacga agccaaagaa caatctgttg aaatgctgtt agaaagcgat
    20821 tcttttatcc ctaaaatgga gatggatgac tccatccatt cacttttgca agtgttaggg
    20881 aacttacttg ataatgcaaa agaagccgtg ctacaaaaac atgaaagaaa cggacaggtc
    20941 cggattcatt taaactatga cacagaagct gaagtattca tcgtgaccgt ctgcgatgac
    21001 ggtgtcggga tatcagaaga aatctcagaa aaaatattta cgctaggttt ttcgacgaag
    21061 ggaatcaatc gtgggtttgg attgaatctc atccattcaa tcgtcaaacg ccacggtgga
    21121 tttttagaat tttccaaaca aacaaatggc ggtacaatcg tctatctaga attaccaaaa
    21181 aaggaagatc aaaatgatga caaatgtcct gataatcgaa gatgatccaa tggttgcaga
    21241 attgaatcgt acttttgtgg aaagcatccc taatatgaac gtaatcgaaa gtttcagaaa
    21301 tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca gatctgctgc tgcttgatgt
    21361 atacatgcct ggcgaaagtg gtatccattt tttatcttgg cttagacagc aaggaaatga
    21421 tatcccagtg attttgatca ctgctgcaga tgatatcgaa accataaaga atgtgatccg
    21481 ctacggtgcc ttagattatt tgatcaaacc gttcactttg caacgttttc agctggcgat
    21541 cgaaaagtat cttgctttct ctcagacaat ccaaacgact gaaaaagccg atcagaaatt
    21601 gatcgatcaa gtattcaacc acaccactcc cattcacgaa ataccatcat tcatcgattt
    21661 gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt gcatttattc taaaagaaga
    21721 tactattttt tcaacaaaag aactggctca aaaaagctca ctgtcacgta tatccgtcaa
    21781 gaaatatttg aattttttag tagaacaaga tatattgaac gaaagaatca aatatttgga
    21841 aatcggccgg ccgctaacct gttatgaatt gacaaagaat gggcggaaaa agctcacaga
    21901 ttactgttga gtcatcgaat tatcccatac aaaatagtcc aagtatacag aagtaacaac
    21961 tgtttacttg gactattttt aaacatttta ttgacgaata tccatgtttt cttatcttaa
    22021 ttgacgggct ttaggaagag atacctcatt tttcgttttt ccagtttcta ttatttcacg
    22081 aaagttctcg aaacttgtcg aaatcatatt tgataatgcc tcatctgtat ttgaacctac
    22141 atgcggggtt acaagaactc tagggtacat ttctactaat tgctggatag cagggtctgg
    22201 aatcgtctcc catggcttga aggatcggaa aaaaacctct gtttcatttg caaaaacatc
    22261 ggttgcaaaa ccagctaaat gattgctttt caaagctctt aaaatagctt cattatcttg
    22321 aagctctcca cgggctgtat taatcaagat gctacctttt ttcatttgag ctaaaaatcc
    22381 ttcattgatc atttgatcgt tttcacctgg gatatgaggt acatgaatac tgacaatatc
    22441 gcttttggct aataactctt caagcgagtg gaacgtcaat acttttttag cagcttctgt
    22501 ctgatgaata tcgtagccga tcactttggc tcctagtccc ttaaacaaaa cagcttccgt
    22561 caaccctatc ctgcctgttc ctatgatccc tactgtgcaa tttctgattt cacgactaaa
    22621 catttgctca tccactcgaa aatctttgaa agatgttcgg atcgtcgtat acgcagtatt
    22681 tctcaaaagc atcatggcaa aagtcaaaga taactctgct atcgcatttg gcgaatacga
    22741 tggtacacgg gctactgtca taccaaaatc agctgccgct tgtagatcga tatgattgac
    22801 tcccacagtc cgtgtaaaaa cataacgaat cccatattcc tgcatttttg caagattggt
    22861 ctcatctgct atacaatttc ccctcaacaa tacagcatca tggtcctttg ctgacacgat
    22921 attgtcatgt gtcaacagtt cttcataaag actgagatca taatggtaaa tattcaattt
    22981 ttcaaaaaaa tctatctcgt tcggacgaac accgtaacat gcgattctca tactttcttc
    23041 ctccatttct tatgaaaata aacctatgcc atgaatcatt tgtaatacaa cgatccatac
    23101 aggtgtcagt accacagcaa aaatagttga cagtaaggat gcattggagg ctaaaagtgc
    23161 ctccctatca aaactgatag cataggctgc tgccactgtt gcagctggtg tagccatcat
    23221 gataacgatt gttgccagtg cttcaaaatt gactggtaga atattcgttg ccgtcaaaac
    23281 tgctagaatc acgatattca atgcaggtac aaataccact ttgactagac tgtaatacca
    23341 tgaagttttt tcagatgcag cttttttgaa gcttacttca cctaatgttg cgccgattgc
    23401 tagccacgcc aatggtgaac ttaaaccagc taaataatcc atcggctgaa atagccacgg
    23461 tgcagtctgg tcaattcgca aaaacgctac ttggcttatc tctccttctg cacctgtaac
    23521 tgacacttgt ggcagatacc cttggaacag ccaaatgaaa aggccggcaa aagtagcgat
    23581 aatgattgga ttgacgaaca tcgctttgat atttttagct tccatcttca agccactcat
    23641 ttttatgtat ccataagaat acaaaaagat tcggtagcca atattgaaaa ttgaggaata
    23701 catcacgccg acaggaccat aaatcgcact gacgataggc gttccgaaaa aggtcgtaga
    23761 accaaagatc gtcaggacac gcaacgttgt ttgcttatcc cctttatagc ttaagaaaaa
    23821 aggttttgaa actagaataa gaataatata aataactaat ccccaaatca agacattgat
    23881 tccttgcatc agtaattctg tattgatatc ttgcatgaaa gctcgaaatg ccagtgctgg
    23941 caaagccact gttaagacga ctttcgatag aatttttcca acagattcag aaaaaatccc
    24001 cttttttcta caaacaaacc ctaataatat aataaaaact gttgaagtga ttgcactgac
    24061 aatcgacata tctgtcaaag tcgatcgaaa aatgtcaaat aagttcatct ttctcctcct
    24121 aattatgttc atttattcac aataaaaaaa gcttttattt tgctaatacg tattaacatt
    24181 tttaatttta tctttacatt gttacttatt gaacaatagg aaattaaaga aacttttgaa
    24241 acttatgtaa agtgatttt
//
LOCUS       DACCYH010000037        23828 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.037, whole genome shotgun sequence.
ACCESSION   DACCYH010000037 DACCYH010000000
VERSION     DACCYH010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.037"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(53..787)
                     /locus_tag="IVY85_001434"
     CDS             complement(53..787)
                     /locus_tag="IVY85_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8044459.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="IVY85_001435"
     CDS             1114..1524
                     /locus_tag="IVY85_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP8044460.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="IVY85_001436"
     CDS             1517..2218
                     /locus_tag="IVY85_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP8044461.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="IVY85_001437"
     CDS             2218..3804
                     /locus_tag="IVY85_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8044462.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="IVY85_001438"
     CDS             3823..4044
                     /locus_tag="IVY85_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP8044463.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="IVY85_001439"
     CDS             4041..4802
                     /locus_tag="IVY85_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP8044464.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="IVY85_001440"
     CDS             5182..5691
                     /locus_tag="IVY85_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8044465.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="IVY85_001441"
     CDS             complement(5766..6305)
                     /locus_tag="IVY85_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP8044466.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IVY85_001442"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IVY85_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP8044467.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7372..8169
                     /locus_tag="IVY85_001443"
     CDS             7372..8169
                     /locus_tag="IVY85_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP8044468.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8197..8832
                     /locus_tag="IVY85_001444"
     CDS             8197..8832
                     /locus_tag="IVY85_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP8044469.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="IVY85_001445"
     CDS             8852..9625
                     /locus_tag="IVY85_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8044470.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="IVY85_001446"
     CDS             10033..10830
                     /locus_tag="IVY85_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP8044471.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="IVY85_001447"
     CDS             10808..11221
                     /locus_tag="IVY85_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044472.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="IVY85_001448"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="IVY85_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8044473.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="IVY85_001449"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="IVY85_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP8044474.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="IVY85_001450"
     CDS             15998..16558
                     /locus_tag="IVY85_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP8044475.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IVY85_001451"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IVY85_001451"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP8044476.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="IVY85_001452"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="IVY85_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP8044477.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="IVY85_001453"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="IVY85_001453"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP8044478.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="IVY85_001454"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="IVY85_001454"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP8044479.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="IVY85_001455"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="IVY85_001455"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP8044480.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="IVY85_001456"
     CDS             21905..22483
                     /locus_tag="IVY85_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8044481.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="IVY85_001457"
     CDS             22535..23017
                     /locus_tag="IVY85_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP8044482.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="IVY85_001458"
     CDS             23134..23508
                     /locus_tag="IVY85_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044483.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7781 a         4286 c         5115 g         6646 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaca
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttt
//
LOCUS       DACCYH010000038        23758 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.038, whole genome shotgun sequence.
ACCESSION   DACCYH010000038 DACCYH010000000
VERSION     DACCYH010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.038"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..213
                     /locus_tag="IVY85_001459"
     CDS             <1..213
                     /locus_tag="IVY85_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044484.1"
                     /translation="GEVAASLARKDEHGLRDGIGDVVVTLIILAMQNDMDLYECLNQA
                     YNEIKGRTGKMVDGVFVKSSDLEDSK"
     gene            210..428
                     /locus_tag="IVY85_001460"
     CDS             210..428
                     /locus_tag="IVY85_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HAP8044485.1"
                     /translation="MISLTIFWCVIGGLLAMLGAYLIYESAKGKKYTAVGIILFVVGI
                     LVAIGAEINLYLNGTTEELMWFLLWTRR"
     gene            441..722
                     /locus_tag="IVY85_001461"
     CDS             441..722
                     /locus_tag="IVY85_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044486.1"
                     /translation="MGDMGEYWKDIKPHLKERRKQHVRKMGNSATKNIETLGFEFTHY
                     PNNHQFAINTHNGIIDYWGTTGTWIERKTKKRGKGIRSLRKYINQEDSK"
     gene            719..1045
                     /locus_tag="IVY85_001462"
     CDS             719..1045
                     /locus_tag="IVY85_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044487.1"
                     /translation="MNYTQQELTDLCPKHVAEFINNEVLPKYADGLNTAENVTDFMIN
                     DAIDRLIFLEIDCIAYYRLHAEVALIDPYIALSQNRKILVAYIDTKQILEGRDVEIEI
                     DEEEVG"
     gene            1042..1239
                     /locus_tag="IVY85_001463"
     CDS             1042..1239
                     /locus_tag="IVY85_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044488.1"
                     /translation="MKAYTIKFYGNKTYVYILSGLRLDFRGYSKEDLLESLYGYGYLN
                     EDELISLEKFTNAWTIEEEAE"
     gene            1236..1445
                     /locus_tag="IVY85_001464"
     CDS             1236..1445
                     /locus_tag="IVY85_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044489.1"
                     /translation="MKIYVVKFGNKFYRSDERSIGANTLFIVDILQSARWFDSLEEAN
                     QVSRRLGGLTQVYELVTVDHEEVER"
     gene            1442..1729
                     /locus_tag="IVY85_001465"
     CDS             1442..1729
                     /locus_tag="IVY85_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044490.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKKRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMKAI"
     gene            2321..2788
                     /locus_tag="IVY85_001466"
     CDS             2321..2788
                     /locus_tag="IVY85_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAP8044491.1"
                     /translation="MVLFDVKKYETPEAKDVDMDRTKHNVGVFLSAYLSARCRIGQPR
                     EPKVTASYSLVPPSTANNTFEAERMMIEREEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            3087..3428
                     /locus_tag="IVY85_001467"
     CDS             3087..3428
                     /locus_tag="IVY85_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044492.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDPRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(3421..3600)
                     /locus_tag="IVY85_001468"
     CDS             complement(3421..3600)
                     /locus_tag="IVY85_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAP8044493.1"
                     /translation="MYFVIREATNGQYYFVIKSDNNEVVATSETYLTKYSAEQTINSI
                     KNGITKDSQVIDVTK"
     gene            3962..4174
                     /locus_tag="IVY85_001469"
     CDS             3962..4174
                     /locus_tag="IVY85_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044494.1"
                     /translation="MNENQLRELFKTNEANKTMEATFYETQKSLALIAKQAKYFYDQL
                     ILQGFNDAQAMEFMMRTFSASNQQKE"
     gene            4181..4405
                     /locus_tag="IVY85_001470"
     CDS             4181..4405
                     /locus_tag="IVY85_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044495.1"
                     /translation="MRNYWYVSLSNKYPQPNADDPIRVVQSVQIKKKYSIIEMTREAT
                     PKEIDKYNLRYCGHGYFSEQNIQTNIERYC"
     gene            4432..4965
                     /locus_tag="IVY85_001471"
     CDS             4432..4965
                     /locus_tag="IVY85_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HAP8044496.1"
                     /translation="MVNLTPKQKAFADEYIKNGGNATQAAIKAGYSKRSARVIGKENL
                     TKPNIIQYINERLNPIEKKRKLSAEDALNELIDIWQGEVQISVSKQIDRLEKNKVIKH
                     MQYEYTPDLESKLKALDLYLKYKSLLSQTQLEKAQTEIKLMQAKLEQLQINSERSTEE
                     KLDELLEKISGELDGTS"
     gene            4952..6196
                     /locus_tag="IVY85_001472"
     CDS             4952..6196
                     /locus_tag="IVY85_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAP8044497.1"
                     /translation="MALVDIYNPKQIDVLNKTIKNDWFITLLHGAKRSGKTKINNDLF
                     LFELRRVRKIADEEDIKEPMYILAGVSSATIQKNILQELYNMYSIEPKFDKHGNFKLF
                     GVKVVQAYTGNIGGVGAIRGMTAYGAYINEASLARQEVFAEIVSRCSGTGARILADTN
                     PDNPEHWLKKEYIDNSSKNIQSFHFELDDNTFLSERYRNNIKESTPSGMFYDRDIKGL
                     WVSAEGVVYRDFDASKHYIQSKDLPPLSNFYCGVDWGYEHWGSIVVIGETDDGTAYLI
                     EEHATQFEEIDYWVDVAKGIQERYGLRVPFYCDSARPEHVARFVREKINAKNAHKARL
                     SGVEEVAKRFKQDKLFICQDRVMKFRDEIYQYIWDKKKGEPIKEYDDVLDSVRYAIYT
                     HELLKKPKVNVNEKIKRIKRMF"
     gene            6209..7708
                     /locus_tag="IVY85_001473"
     CDS             6209..7708
                     /locus_tag="IVY85_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8044498.1"
                     /translation="MDKVNEFEYGADIHYSNDVNTNYVKFSVDSNLHYRFSSAEDLLN
                     DLDTLAAMIKHHHEYQVKRLSVLDDYYKARNTNIMDNRRRREKEKADHRSAHNFGKVL
                     CTFDVGYNTGNPIKVQIEDTNQQKEIEEFNTNNDIDGLNAELWLDMDKYGRAYEIIYR
                     DSDDTDYVDLANVFETFVVYDTTVKREPILAVRYPKTRFNKDADKQYIQPIVYTKEKS
                     ITYDETTLTAIELKNPQDEPHEYKEVPITEYSPNRFRMGLYEDVLSLIDLYDAGQSDT
                     ANYMTDLNDALLVISGDIEAAGLSTEDAIKQKEANMLLLESGTDVNGNKTSVTAGYIY
                     KQYDVNGVEAYKYRVRKDIHEISMVPDLTDDNFSGVQSGEAMKYKLFGFEQMTATKQR
                     LFKKGLMRRYRLLFSLKSSISEMDNSDLKGLRVIFTPNLPKAILEELKSLVDAGAELS
                     QETILGLASFVPDVQAELKRVNKETQKQIGIFDSDGEEVINNKKDETGG"
     gene            7713..8639
                     /locus_tag="IVY85_001474"
     CDS             7713..8639
                     /locus_tag="IVY85_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAP8044499.1"
                     /translation="MNSQEYWIKREKEWQKQQIKDDKKRMAEIKSRMQYAQDAIQKEI
                     DAQWDSFSNGQKITRSEAMKRASEMDVKAFARKAKKYVKEKDFSPTANQELKLYNLTM
                     RVNRLELLKANIGLELISLFNELDKYFSNELTKAGLAELKRQAGILEMTIASSGYAKL
                     IELVINSSFLSDDVSFSDRLWMYQSELKSELDRLLVRSITMGKNPKQLASKLAEYLTA
                     EGRENTKFNTQRLMVTETTRVQVGIQERSYRDAGITQYIYIAEPTACKLCIPLNNQVF
                     DVADMQPGSNAPNMHPFCRCSTAPYIERISSR"
     gene            8766..9368
                     /locus_tag="IVY85_001475"
     CDS             8766..9368
                     /locus_tag="IVY85_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAP8044500.1"
                     /translation="MKKRLFMPMNLQFFSEPGDGGSGDEGQQGNLPAGSQETPPEAKE
                     ENNTGKTFSRDEVAKMIAAETNKAKAAWEKELAAKKEEAKKLAKMNAEEKLQHELEQK
                     EAEIAELKRGQALSEMTKEASKMLTDANLPHDDDLLGLIVSDDADATKQAVAVITNFA
                     SLIKRENARQTPPNEGGQFTASKNTKETVAKLAAKNRIIK"
     gene            9381..10304
                     /locus_tag="IVY85_001476"
     CDS             9381..10304
                     /locus_tag="IVY85_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8044501.1"
                     /translation="MKKKQLLPMNLQMFAQTWDPDNVLVYETKEGKIPDKYNTLILSE
                     VMENSKIMQLAKYEEMTDKEKKFEYFAEGPGAYWVGEGEKIKTSKPKWMQATMTAKKL
                     GVILPVSREYLNYKLSDFFEEMQPKIAEAFYKKFDAAALLNKENPFPQSLDGSVISAG
                     NVVEGGLTYDNILALEDKLAENEFEPNAFISNRKNRTELRSAAQTVGSNVEFIYDRST
                     NTIDGLPVVDLKSLDKGTLYAGDFNYMFYGIPYNISFKISEEAQLSTLTNEDGTPVNL
                     FEQELIALRATMDVGFMIVKDEAFGKISPKA"
     gene            10322..10585
                     /locus_tag="IVY85_001477"
     CDS             10322..10585
                     /locus_tag="IVY85_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044502.1"
                     /translation="MPNQKTWTGKVGDTKTFTISAVPADASDAAAVVAATTATSSDGA
                     IATVTKNENGGFDGTIAAEGSATFTFTSGEFTTSINVTGQPAS"
     gene            10597..10926
                     /locus_tag="IVY85_001478"
     CDS             10597..10926
                     /locus_tag="IVY85_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HAP8044503.1"
                     /translation="MMIADDIKKLLKGTIDEKLEVIERRTNERMKTLLNTQEVPKEFE
                     TVVYEVSLKRFNRIGQEGMQSYSQEGLSMAFPDSDFSEYQNEIDEFKRKDQEELYKPK
                     RGRFKFV"
     gene            10923..11249
                     /locus_tag="IVY85_001479"
     CDS             10923..11249
                     /locus_tag="IVY85_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044504.1"
                     /translation="MRFTDEIIFVKRSSDSKYDPDLGEWVEGKPERTRTEANVTDIGT
                     DRSVTIFGSVEEGAKVIRTQPLFSIPTFDYIEIEGKTWQQKTARNPAYRNSLIVQEVV
                     LDEGTT"
     gene            11233..11571
                     /locus_tag="IVY85_001480"
     CDS             11233..11571
                     /locus_tag="IVY85_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP8044505.1"
                     /translation="MKAQLEYKGIDQLMRHLKKAATLNDVQKVVKSNTAEMTERMQKG
                     APVDTGYLRRSINMNLLEAGLTGIVGPTADYAPYVEYGTRFMSAQPYVRPAFNYQKVK
                     FMAEMKALVK"
     gene            11571..11963
                     /locus_tag="IVY85_001481"
     CDS             11571..11963
                     /locus_tag="IVY85_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HAP8044506.1"
                     /translation="MIKTRDQSIFDELFKISQNKLGYKTYDYKTLEDVGYPFVEFENT
                     QTIHEVNKTDIKGSVIVVLSVWGLQKKRKQLSDMASALFNEARLIEATEGYYWALNYQ
                     ASGIQVMDDTTTNTPLKRAVVTLEFRIR"
     gene            11974..12486
                     /locus_tag="IVY85_001482"
     CDS             11974..12486
                     /locus_tag="IVY85_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HAP8044507.1"
                     /translation="MEALKGIDVILLYRLLKKETQEAAWKMAFQTEHENGLSRDSDST
                     VTKDGNVQSLSPVEYDFSATSIVAKGDSHVDEMKQALLNGDIIEIWEINKAEQGTDDN
                     ANKYKATYYQAYVSEFTPSAAAEDNVELSLSFAVNGVGQDGYATLTEDQADVVQYAFK
                     DTVKATSTGA"
     gene            12532..12900
                     /locus_tag="IVY85_001483"
     CDS             12532..12900
                     /locus_tag="IVY85_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044508.1"
                     /translation="MKLKIKGKEYSFKFGTKFVRELDKVMPFVDGNMEFGMGLSAKVL
                     PELRSYNVNTLSRVLEIANRTEDESITLDELDDYIDEVKDIEKLFDEVLKELAESNAG
                     KLAVRNLNQKLKEAEKQQVE"
     gene            12954..13241
                     /locus_tag="IVY85_001484"
     CDS             12954..13241
                     /locus_tag="IVY85_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044509.1"
                     /translation="MTNISDIERMTLYEYNIRMTAAQLSWLDKEKLIHELAWANQQVQ
                     AEKKVGKKTVPVYRSFEEFFNYQKIEDSIMGVSELSKQDKKFQSLLTKANS"
     gene            13258..16377
                     /locus_tag="IVY85_001485"
     CDS             13258..16377
                     /locus_tag="IVY85_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8044510.1"
                     /translation="MEQFSVEALLKATDSGFVKTFKDAQDAVKTFEKNSNSMTTAVGK
                     VMQGTGAAMTKYITTPLIGVGVAAAKVGGDFEAQMSRVKAISGATGDTFEQMKQQAID
                     LGAKTAFSAKESAAGMENLASAGFSAQEIMKAMPGLLDLAAVSGGDVALASENTATAL
                     RGFGLEASEAGHVADVFARAAADTNAEVGDMGEALKYVAPVANSMGISLEETAAAIGI
                     MSDAGIKGSQAGTTLRGALSRLARPTKAMQDTMDNLGVSFYDADGKMKPLKTQVELLK
                     KAFEGLTPEQQQNALVTLYGQESLSGMMALIDKGPDSLGKLTKSLKDSDGAADDMART
                     MQDNMNSSIEQMFGAFESAAIVIQKILAPSIKKVADAISGLVEKFVSAPESTQKLVVA
                     IGAIVAAIGPLIFMIGSVIIWINRVKVAFKALSESSKLFSGLSKAMGLLTNPVFLVIA
                     AVALLVVGFIYLWNTSEDFRNFWIGLWEGIKSAVSSAVEWIQNAWKSTGEWFNNLWKS
                     IKEGADNVWTTVQEAPGKAADWIKNKWTGTKEFFSNLWSSIANSASEIWNSLKEGVIS
                     VIDDLVSSAGEKWEGFKNTISTAWKTITSKIKSGFDFILKYIGPFVSSFSDVFSNIVK
                     AITSIFAEVKNIIVNAWEIIKSLIAAPLLFIIDLITGDFEQMKEDLDLIWNTLVQSVV
                     NIWTSVKNIFTEYIGAIVNSAVSLWTGFIQSISNIWNEVVYQATMIWIDLKLFFTNLW
                     IDIKYSAIQMWINLKFSIIQTWIDTKYGAIELWNNLKQWFFQTVNNIVQTLIKSWNSL
                     KQGTIDLFNNTVQGAKDIWTSFKSWIGDLITGTKDNIIQGWENLKQGTIDTFNNLVNG
                     AQEAWDNLVNAVSDTVDRVTGWFDNLKNIDLLAAGKAIMDSFLEGLQNAWKSVQDFVG
                     GIGDWIREHKGPIQYDRKLLIPAGQAIMNGLNKGLTGGFNDVQNTVGSMADFIAELFN
                     ANSDVDIAANLKNANKNIGAQVEHKINMGGSTKPAVFKINLGRQSFRLFMDDISQAMG
                     EGADINLEF"
     gene            16410..17177
                     /locus_tag="IVY85_001486"
     CDS             16410..17177
                     /locus_tag="IVY85_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8044511.1"
                     /translation="MYSFKDTTINLTSSKRFLPTSAMMYDGMYLEDLIDGYQTLTVEG
                     REMLSVEVEQQEIQIGSIITNQKIPSRTLKITYKLEDRDPEKLQFKFKELLNYLYRNE
                     DVEIRFHDELDYYYYGRYTSTDTVPGDSNSIISSFNVFCADPLKYTKECVSDGYIGNP
                     IQFPITPRKIEVTLSMNNSIKITNGEQNITITDAAIKTGDVLVFDFSDEQVTVNGEDC
                     TSMIDLESDFENFYLKQGQKITSNNGKLKIFYRGATI"
     gene            17174..20005
                     /locus_tag="IVY85_001487"
     CDS             17174..20005
                     /locus_tag="IVY85_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8044512.1"
                     /translation="MSETVYFFDHLQKLIKRKNTRSLIEVSQEKEISSDKSDLMKDTL
                     YVTTKYDKEIEDARYMAIRENESSFSLYRITKVSDPFETLEFTGLGFATNELDAYIIK
                     DIRPSGQPLKNVLDRLIEFTEGNWRVGHVEAMLPTVTATFYYVSVKEALKELQTLGME
                     FVFRCSLNSDGIKDKWIEVYEQIGEESNTRFVYGSKALTVVREIDRSSISTSMIGRGR
                     GEEVGDGYGRRIEFTDVEWKKSNGDPLDKPKGQNWLEDPESTQKYGIPQKDGSMRKRE
                     TVVVFDDIDDPTDLLKNTYSTLIDSARPLVQFKAEVTGGDVIGNTVTIHRYDKGYHYK
                     TRIYKTTFNRLTGQTNIELGDNLTQDVRKQTASIVNNINSLESSKMTFYESTEIGKYQ
                     DDIMRGAGDNGGSIYWVNGIEAGVSDSREIYETVYMDGPNIPRSRFFMVQNNSGISFK
                     QCKKGEWQTIQDVHNGDSTTAWTLDGTFNANFIRAGILSGILVQGVALKTFDDKDFQL
                     VAEGGELSFEKQVKSTGLDDVHGERLGSIVSTYGSKGINGFAVWKEPNYIFSINAGDG
                     GDRGNPIFQIPADVTADKRKYNLYGDGNFSEGNITIDGRLDVKELYVNGTKIDTNGGG
                     NSGGNDNGWNGQYPPEVTTDRDKRYWQIWATAIGAGFTQQAAAALLGNAQGESDANPT
                     ADEGNGAPGFGYGVWQWTDSSGATSGRVYMINLMTKAGISDDPDTITAQFKLLMWHAP
                     NGQWIATSAYPYTWTQFMNLTDINTAAQAFVANFERPRDPHPERTAWSQEWYDKFKDL
                     EIPVSKGYMKPIADPITVTSEFGWRTSPITGAQEFHNGIDLVNGNPNTPIFASADGEV
                     IVAGDANYFDWYGNWTVIKHADGMYTGYAHQSRVDVSKGQKVTAGQQIGLMGTTGPST
                     GEHLHFQFMDEFYPSSSGHFHNARDYIDF"
     gene            19980..21737
                     /locus_tag="IVY85_001488"
     CDS             19980..21737
                     /locus_tag="IVY85_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAP8044513.1"
                     /translation="MQETTSISKGGIVMAETQHKMVLSTTEPNNGINLVRIRQGDVLT
                     QKFVVEVVEHGKLKTFEGLVPFFINTTKFGENQPVEQKVQEYSPAQARLVYTLSEPDW
                     QWGGENTAHFSFRSLNGDGTWSEQFSTQDFTYRVISGISRSQLRDSGYVWTFEDLLRK
                     FKDYMDQGKNDWEQWLEDNREILENIDPGGMIINILNEAKGDYESLADRLNQKYQVPV
                     GSSQIRETTRFFDYDTMKYVDLVPRNLNTVVNSVNNSKFNFSFITDIHADNHNLRIDG
                     VGYKDASYLRHWRAIPQFQKLGNKTDVMIYGGDNIDGGLGSLGSDIGIIDEWSARHSM
                     LGTLKRFTNAAVAGQEKPVIICKGNHDACFDPAWRKRKGMLCNADFEEYWNGLYGGVL
                     FPDKNAAIYRFDTCDFYEGGTGDKYVDGYSDTAPGAFSAKQINAFGEWLVNVPRNYHV
                     ALVGHTPLGLSKFPVRNENMISTLIEGFKSGSPVTIDWSKLGQPNDGSFGGLKTFAMN
                     TKGAGVVVGYFCGHWHEQVEGAFGTVKMILCDVGFSHTASQVDTPDELAFYKIEVDTA
                     TRKVTSKGVGRARDFTYNY"
     gene            21754..22536
                     /locus_tag="IVY85_001489"
     CDS             21754..22536
                     /locus_tag="IVY85_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044514.1"
                     /translation="MLDFQSKPNIFEEMSYEEAVKWLLRQAAIHYDGSDHDAHVLATE
                     SNAGFATPETVMQARGRWLRDYKLPQKYPNILDIPPGKYATKAGWGADNPDGIEDDSF
                     VEMMVFADHDSRKLIVAFARYSGEIYIKMTHNSEPVEGYNSLGWRRVYTTSVLFEGEL
                     RKGQSVNLPDDTFRYQTLRIHYTDGDGDFVEEVKRQRYARITKANLWNGDAGVTFIEF
                     ELTIEARKITMSNGRALDISSGNVSNPAMSNNVKITKIEGVK"
     gene            22536..>23758
                     /locus_tag="IVY85_001490"
     CDS             22536..>23758
                     /locus_tag="IVY85_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAP8044515.1"
                     /translation="MAHVIKKGSIKVPTQPKDYDLQATGLVFKSYDNQIALEFNVEQQ
                     NGTPADLLGANLRLLMFIYDEVDGMITKEPIPFITKNLITESFLNGQVVYILPEAMKA
                     YNGMVEAYVYIEYPDGSTSDNLGFTFRMKRSAIDGLAQDKADYFIADFQQLLDGVKQE
                     ATDAVNAALTNVDSTIEKANQQINEFVEGATQAIDQTVDEVTEQLQATQTKIDTVSQN
                     VTSAQNNLKAVEDKMNQTNQQIGDLGKLKKMYSNSIDFGNYDYSGNPNLMSKLKSSDF
                     NVGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGKTYTLSAKVRFDEGTTGAI
                     DKLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFV
                     DRDKINGGFKLYDIK"
BASE COUNT         8351 a         3791 c         5144 g         6472 t
ORIGIN      
        1 ggggaagttg ccgcttctct agctagaaaa gatgaacatg gtttaagaga cggaattgga
       61 gatgtagtag taaccttgat tattttagct atgcaaaatg atatggattt gtacgagtgt
      121 ctgaaccaag catacaacga aatcaaagga cgcacaggaa aaatggtaga tggtgtattc
      181 gtgaagtcga gtgatttgga ggacagcaaa tgatcagcct aacaatattt tggtgtgtaa
      241 ttggcggctt actagcgatg ttaggagcat acctcattta cgagagtgca aaaggcaaaa
      301 aatatacagc agtagggatt attttgtttg ttgtcggaat tttagttgcg attggtgcag
      361 aaataaatct ttatttaaac ggtacgacag aagagttgat gtggttttta ttgtggacaa
      421 ggagatagga ggacagcgaa atgggcgata tgggcgaata ctggaaagat attaaaccgc
      481 atctaaaaga acgtagaaaa caacatgtca gaaagatggg caattcagca actaaaaata
      541 ttgaaacctt gggatttgaa tttactcatt atccaaacaa tcatcaattt gcaattaata
      601 cccataatgg aattattgac tactggggaa ctacaggaac gtggattgag cgcaaaacaa
      661 aaaaacgtgg taaagggata cgtagtttga ggaaatatat taatcaggag gacagcaaat
      721 gaactataca caacaagaac tgactgattt atgtccaaaa catgtagctg agttcatcaa
      781 taatgaagtc ctgcctaaat atgcagatgg tttaaataca gcagaaaacg ttacagattt
      841 tatgattaat gatgctattg atcgtttgat atttttagaa atagattgta ttgcttatta
      901 tcgtctacac gcagaagttg ctttgattga tccgtatatt gctttaagcc aaaatcgaaa
      961 aatactcgta gcctatattg ataccaaaca gattttagaa ggcagagatg tcgagataga
     1021 gatcgatgag gaggaagtgg gatgaaagca tacacaatca agttttatgg aaataaaacc
     1081 tatgtataca tcctaagcgg tctcagactt gattttagag gctattcaaa agaagacctt
     1141 ttagaatcct tatatggtta cggatattta aatgaagatg aactaataag cttagagaaa
     1201 tttacaaatg cttggacgat tgaggaggaa gcagaatgaa gatttatgta gtaaagttcg
     1261 ggaataaatt ttatagaagt gatgaacgct ctataggggc taacacatta ttcattgtag
     1321 acatactcca aagtgcgaga tggtttgata gcctcgaaga agctaaccaa gtttcacgac
     1381 gacttggtgg attaacacaa gtatatgaac tggtcactgt cgatcatgag gaggtagaaa
     1441 gatgaaatta aaagacggat tttacgctag tagtcatggt atcggcggtt taatgctaga
     1501 tatgccgaca aagaatccta aaaaacgtaa gaaaccaaaa ttcaaagtcg gtgacatggt
     1561 tcgctgcgaa gcagaagggt tcatctatcc atttcgtgga tatgtagaac acgtctataa
     1621 tcactcagcg atcattcgta ttgaaaacac gatggaatgt gataagtggt tagcgaaaag
     1681 caaagagaat ttagctgtag cgagattggt ggatatgaag gctatataaa cagcgaaaaa
     1741 atgtacttct aagagaaaaa tttatttttt ataaaaatag gaaagtatca ttatgaaaaa
     1801 gaccacagag ataaataaac ctaccacata aaaaatgaat atttcttgcg attcaatata
     1861 ggtatattta acaatttgta caataaaaaa tactataggt aaaaaagata acatgaggag
     1921 cttatttgtt ctagagtgtt tgacaaagta acaaaatgcg acgataccaa tacaaacggg
     1981 aatccaaaat aacagcatgc tccacatagt cactcaacca tctcctatac gctttattat
     2041 caatagataa tatcattttt cgtaataaag ataaaaaaat aagtaaggct tctcattaaa
     2101 atataaaaaa gacagccgac cactggctgt ccttagcaga atattgaaat agtgagccat
     2161 ctgttttccg ccagatagct caacatgtat atgggtaacg agtgcaacct cgctacttga
     2221 aaagcttagc ataagcatgg gtataaaaaa agccggattc ctccgacctt aattaataat
     2281 tctgacacaa ttattatatc ataaatggag gaatcaatgg atggtacttt tcgatgtaaa
     2341 gaagtatgag acaccagagg caaaagatgt ggatatggat cgcacaaaac ataatgttgg
     2401 tgtttttctt tcagcatatc tgtcagctag atgtaggata ggtcaacctc gggagcctaa
     2461 agtaacggca tcttactcct tggttccacc ttctacagca aataatacat ttgaagcaga
     2521 acgaatgatg attgagagag aggaagcgca agaagaattt gagtacttgc acaaattatt
     2581 tgttcgtggc tattcagcaa ttcagcatcc gcataaacct gatgtaacag aaaggcgcaa
     2641 gaagatattc tatgatcgtt atatcaatgg tctgcccatt tatgtaactg ctcaaaggaa
     2701 taatactagc gaagaatcgg ttaaagtaga atcaaacaga attatcatcc aatttgcttc
     2761 atcgttagaa ctggttgctt ttaagtagcc agtttttaca ctttttatac cctttgatta
     2821 cccatttggt ttccatttta tacctttttt ataccaatca cttacctggt aaatgattta
     2881 ttatgatagt gtcgaaagat tagtgatagg tctgagacaa aataataata aaaggaacat
     2941 cgttttatta ttgtttcaca attaagcttc gatagacagc aacggaaata ttaagaataa
     3001 ggatgtgaat tccaactcct tctaaattgt tcttattatc tatcatccgt tgctgtttat
     3061 taatttatgt attggaggga aaacgaatgg aagtcgagca aataaagtat caaggtatgg
     3121 atcgggaacg gttgattgat attattgagc aacaacgttt agaattgatc caaaaagaag
     3181 ctgtatgtaa agagtacaaa aaacatttag aacaagtaat tgagtatcat tcagtagaaa
     3241 agtacagatc agtggtgcaa aaaaatagag caacgagtcc tgatcctcgt caagatgtat
     3301 cgattagtat caagacgcca gtatttaatt ctgttaaatc agaaaagcta gaaagtatct
     3361 caactgcttt ggtagaaaaa gcagctgaaa tgaactccct agcaaatgaa gttagtcgtc
     3421 ttatttagtc acatcgataa cttgagagtc tttggtgatt ccgtttttta tagaattaat
     3481 ggtttgttca gcggaatatt tagtcaagta tgtttcgcta gttgctacta cttcattgtt
     3541 atcggactta ataacgaagt aatattggcc attagttgct tctcttataa caaagtacaa
     3601 atttttcacc accttttaat ttatttcagc ggaccactcg ctgataaata aaattataca
     3661 cttagtattt attttcacaa tattaatttg tcactgtggc ggaaagggta gacgctaagc
     3721 atgtgtgtta ggtcaatgct tcggcaacca tgcaaggttc gattccttgc cagtgacttt
     3781 ggcaactgag ggttggaaat gggcgctcaa agtacacgag caaggcgagg tcgatagtaa
     3841 tcgatggaat cggtgtaggt tgcttgatag aactataact gcatctcgtt tatgaggtgt
     3901 agtttttaca tattagatca ctcatcgagt ggtcttttta ttttggaagg ggagtaaaaa
     3961 aatgaacgaa aaccaattaa gagagttgtt taaaacgaat gaagcaaaca aaacaatgga
     4021 ggcgacattc tacgaaactc aaaaaagctt agcgttaatc gcaaaacaag ctaagtattt
     4081 ctacgatcag cttattctgc aaggatttaa tgacgcacag gctatggaat ttatgatgcg
     4141 aaccttttct gccagtaacc aacagaaaga gtgatacgta atgaggaact actggtatgt
     4201 atcattatca aataaatatc cgcaaccaaa cgcagatgat ccaattagag ttgtccagtc
     4261 agtccagatt aaaaagaagt actccatcat tgaaatgacc agagaagcta cgccaaaaga
     4321 gattgataag tacaatcttc gttactgcgg ccatggatat tttagtgagc agaacataca
     4381 gacaaatatt gagagatatt gttaataaaa ttgaaaggtg gtggcttgaa tgtggtaaat
     4441 ttgacaccaa aacaaaaagc ttttgcggat gaatatataa aaaatggtgg taatgctact
     4501 caagccgcca ttaaagcagg ttatagcaaa cgatcagcaa gagtaatagg taaggaaaac
     4561 ctaactaaac ctaacataat acagtatatt aatgaacggc taaatcctat cgaaaagaag
     4621 cgcaaattaa gtgccgagga cgctttaaat gaattgatag atatttggca aggagaagta
     4681 caaataagcg tgagcaagca aatagaccgc ttggagaaaa acaaggttat taaacatatg
     4741 caatatgaat atacaccaga tttagaaagc aaattgaaag ccttggattt gtatttgaag
     4801 tataaatcgc tgttatcaca aacgcaatta gaaaaagctc aaacagaaat aaaattaatg
     4861 caagcaaaat tagaacaatt acagataaac tcagagcgtt ctaccgaaga aaaacttgat
     4921 gagttgttag aaaagattag tggtgaatta gatggcacta gttgatattt ataacccaaa
     4981 gcaaatcgac gtgttaaata aaaccattaa gaatgattgg tttattactt tattacatgg
     5041 agcaaaacgt tctggaaaaa caaaaataaa caatgatttg ttcttgtttg aattgcgacg
     5101 tgtgcgaaag attgccgatg aagaagacat taaggaacca atgtatatcc tagcaggagt
     5161 ttcaagtgca acaatccaaa agaacatctt acaggaacta tacaacatgt acagcataga
     5221 acctaaattc gataaacatg gaaactttaa gctatttggc gttaaggtcg tacaagctta
     5281 tacaggaaac attggcggtg ttggcgcaat tcgtggtatg acagcatatg gcgcttatat
     5341 caatgaggca tcgctagcta gacaagaagt atttgctgaa atcgtttcac gttgttcagg
     5401 aacaggagcg agaatactag ctgatacaaa ccctgacaac ccggaacact ggctaaagaa
     5461 agagtatatc gacaattcaa gcaaaaatat tcaatcgttc cactttgaat tagatgataa
     5521 tacattttta tctgaacgat atcgtaacaa tattaaagaa tcaacaccaa gcggcatgtt
     5581 ttatgatcgt gatatcaaag gtttatgggt ttctgcagaa ggtgtcgttt atcgtgattt
     5641 tgatgctagc aagcactaca tccagtcaaa agacttacca cctttgagca acttttattg
     5701 tggtgttgac tggggatatg aacactgggg ttcaatcgta gttataggtg aaacggatga
     5761 cggaacagct tatttaatcg aagaacatgc tactcaattt gaagagattg attattgggt
     5821 agatgtggca aaaggcattc aagagcgtta cggcttacga gtaccttttt attgtgactc
     5881 tgcgagaccg gagcatgtgg ctaggtttgt aagagaaaag attaatgcta aaaatgctca
     5941 taaagcacgg ttatctggag ttgaggaagt cgccaagaga tttaaacaag ataaattgtt
     6001 tatctgtcaa gatagagtga tgaaatttcg ggatgaaatt tatcaataca tttgggacaa
     6061 gaaaaaaggc gagccaataa aagaatatga tgatgtgcta gactccgttc gatatgcgat
     6121 atatactcat gagcttctta agaaaccaaa agttaatgtc aacgaaaaga ttaaacgtat
     6181 taaacgcatg ttttaaggag tgtgaaaaat ggataaggta aacgagtttg aatacggagc
     6241 tgatatacat tattctaatg acgtgaacac aaattatgta aagtttagcg tagactcaaa
     6301 tcttcactat agatttagct cagcagaaga tttacttaac gatttagata ctttagcagc
     6361 aatgataaaa catcatcatg aatatcaggt aaaaaggcta agtgtattag atgattatta
     6421 taaagctaga aatacaaata tcatggataa ccgtaggcgc agagaaaaag aaaaagcgga
     6481 tcaccgatca gcacataact ttggaaaagt tctttgtacg tttgatgttg ggtacaacac
     6541 aggcaatcct ataaaagtgc aaatcgagga cacaaatcaa caaaaagaaa tcgaagagtt
     6601 taatactaat aatgacatag atgggttaaa tgctgaactc tggcttgata tggataagta
     6661 tgggagagcc tatgagatta tctatcgaga ttcagatgat acagattatg ttgatttggc
     6721 taatgtattt gaaacgtttg ttgtatatga tactacagta aaacgagagc ctattttggc
     6781 tgtacggtat cctaagacaa gattcaacaa ggatgctgat aaacagtaca ttcaaccaat
     6841 cgtatacaca aaagaaaaaa gtatcactta tgatgagacg acactaacag caattgagtt
     6901 aaagaatccc caggatgaac cgcatgaata taaagaggta cctattacag agtattctcc
     6961 taatcgtttt cggatgggct tgtatgaaga tgtactatct ttgattgatc tatacgatgc
     7021 agggcagtct gataccgcca attatatgac tgatctaaac gatgctcttc tagttattag
     7081 tggtgatatt gaagcagcag gactatccac agaggacgcc atcaagcaga aagaagcgaa
     7141 tatgcttttg cttgagtctg gaactgatgt gaacggtaat aaaacaagtg tgactgcagg
     7201 atatatttac aaacaatatg atgtgaacgg tgtagaagca tacaaataca gagtacgcaa
     7261 ggatattcac gaaatatcca tggttcctga tcttactgat gacaattttt ccggagtgca
     7321 atcgggagaa gcaatgaaat ataaattatt tggatttgaa caaatgacgg caacaaagca
     7381 aaggctattc aaaaaaggcc ttatgcggcg ttatcgtctt ttatttagcc taaaatcaag
     7441 tatttctgaa atggataact ccgatttgaa aggcttacgt gtaatattta cgcctaatct
     7501 acctaaagcc attctggaag agttgaaatc tttggttgat gctggagctg aactcagtca
     7561 agagacgatc ttaggactcg cttcttttgt tccagatgta caggcagagt tgaaacgagt
     7621 aaataaagaa acgcaaaagc agattggcat ttttgattca gatggtgaag aagtaattaa
     7681 caacaaaaaa gatgaaacag gggggtgatt aaatgaactc ccaagaatat tggatcaaac
     7741 gggaaaagga atggcaaaag caacaaatta aagatgataa aaagcgcatg gcagaaatta
     7801 aaagtcgcat gcaatacgca caagatgcga tacaaaaaga aatagacgcg cagtgggaca
     7861 gtttctccaa tggccagaaa atcactcgta gcgaagcgat gaagcgtgct agtgaaatgg
     7921 atgtcaaagc attcgctcgc aaagcaaaga agtatgtcaa agagaaagat ttttctccta
     7981 cagcaaacca agaattaaag ctatacaatc ttacgatgcg tgtaaataga ttagagctct
     8041 taaaagctaa tatcgggctt gaattgattt cactgtttaa tgaattggat aagtactttt
     8101 cgaatgaatt aacaaaagct ggtttagctg aattgaagag acaagccggt attttagaaa
     8161 tgactattgc ttcaagtgga tatgcaaagc tgatagaact agtaataaac agctcctttt
     8221 tgagtgatga cgtgtctttt agtgatcgct tatggatgta tcaatctgaa ttgaaatcag
     8281 aattagatag gttgttagtc agaagtataa cgatggggaa aaatcccaag caacttgcat
     8341 ctaaattggc agaatattta acagctgaag gacgagaaaa cactaagttc aacactcaac
     8401 gtttgatggt gactgaaacg actagagttc aggtagggat tcaagaacga agttatagag
     8461 atgcaggcat tacccagtac atctatatag cagaaccaac ggcgtgcaaa ctatgtatac
     8521 cgttaaataa tcaagttttt gatgttgccg atatgcagcc aggaagtaac gctcctaata
     8581 tgcatccatt ttgtcgatgc agtacagcac cttatataga acgaatatca agccgttaac
     8641 acaaattaac ggctttttat tgtgccttct tacagcttac aggcgttaaa gagaaagcta
     8701 ttttcggctg accggcgtaa ctggtcaaat ttatcgggta gcggcgtaac cgtggaggat
     8761 taatcatgaa aaaacgttta tttatgccaa tgaacttaca atttttttct gaaccaggag
     8821 atggtggatc tggtgatgag ggacaacaag gaaacctacc agctggctca caagagacac
     8881 cgcccgaagc aaaagaagaa aacaatactg gcaaaacatt ttctcgtgat gaagtagcga
     8941 aaatgatcgc tgctgagacg aataaagcaa aagcagcgtg ggaaaaagaa ctagcagcaa
     9001 aaaaagaaga agctaaaaag ctggcaaaaa tgaatgcgga agaaaaacta cagcatgagt
     9061 tggagcaaaa agaagctgaa atcgctgaat taaagcgtgg acaggcacta tctgaaatga
     9121 cgaaagaagc ttctaaaatg ctgacagatg caaatttacc acacgatgat gatttacttg
     9181 gtctgattgt ttctgatgat gcagatgcca caaaacaagc tgtagcagtc atcactaact
     9241 ttgcttcttt gattaagaga gaaaacgcaa gacaaacacc accaaatgaa ggtggacaat
     9301 ttacagcatc gaaaaatact aaagaaacag tggctaaact agctgctaaa aatcgaatta
     9361 tcaaatagga ggaaaactta atgaaaaaga aacaactttt accaatgaac ttgcaaatgt
     9421 ttgctcaaac atgggatcca gataatgtct tggtatatga aacgaaagag ggaaaaattc
     9481 ctgataaata taatacgctc attttgagtg aagttatgga aaattctaag atcatgcagt
     9541 tagcaaaata cgaagaaatg actgacaaag aaaagaaatt tgaatacttt gcagaaggac
     9601 caggcgcata ctgggtgggt gaaggtgaaa aaattaaaac gtctaaacct aaatggatgc
     9661 aagctacgat gactgcaaaa aaactcggtg tcattcttcc ggtttctcgt gaatatttaa
     9721 attataaatt atcagatttc tttgaggaga tgcagccaaa aattgctgaa gctttctata
     9781 aaaaatttga tgcagctgcc ttattaaata aagaaaaccc atttcctcag tcactagacg
     9841 gatcagttat tagtgcgggg aatgtggttg aaggcggatt gacttatgat aatatcctag
     9901 ccttagaaga caagttagca gaaaatgaat tcgaacctaa tgcgtttatt tcaaaccgaa
     9961 aaaatcgtac agaattacgt tctgcagctc aaacagtcgg gtcaaatgtt gagtttattt
    10021 atgatcgctc tactaataca attgacggat taccagtagt agaccttaag tctttagata
    10081 aaggaacact ttacgctgga gactttaatt acatgtttta tgggatccca tataatattt
    10141 catttaagat ttctgaagaa gcccaattgt ctactttaac taatgaagat ggaaccccag
    10201 ttaacttgtt tgagcaagaa ctgattgctt tgcgtgcaac aatggatgtt ggatttatga
    10261 ttgtaaaaga tgaagcattt gggaagattt ccccaaaagc gtaacgcctg ctaccggtat
    10321 tgtgccaaat caaaagacat ggaccggtaa agtaggcgat actaaaacat ttactatttc
    10381 agctgtgcct gcagatgcta gcgatgcagc tgctgttgtt gcagctacta cagcaacttc
    10441 aagtgatgga gctatcgcaa cagtgaccaa aaatgaaaat ggtggttttg atggaacgat
    10501 tgcagcagaa gggtcagcaa cattcacatt tacttctgga gaattcacta cttcaatcaa
    10561 tgtgacaggt caacctgcta gttaggaagt aaaaatatga tgattgcaga tgatattaaa
    10621 aaacttctta aaggaacaat agatgaaaag cttgaagtta ttgagcgaag aacgaatgag
    10681 cgtatgaaaa ccttgttaaa tacgcaagaa gttcctaaag aatttgaaac agttgtatat
    10741 gaagtatcgt tgaaaagatt caatagaatt ggtcaagaag gtatgcagtc atattctcaa
    10801 gaaggtttat ctatggcttt tcctgactcg gatttttcag agtatcaaaa tgagattgat
    10861 gaatttaagc gtaaagatca ggaagagttg tacaagccaa agcgagggag gtttaaattt
    10921 gtatgagatt tacagatgaa attatatttg ttaaacgttc atctgactct aaatatgatc
    10981 cagatctcgg tgagtgggtt gaaggcaaac cagaaagaac aagaacagag gcaaacgtga
    11041 cagatattgg cactgataga agtgtgacta tttttggtag tgtggaagaa ggggcgaagg
    11101 tcattaggac gcagcctctt ttttctatcc ctacatttga ctatatcgag attgaaggaa
    11161 agacttggca acaaaaaaca gctagaaatc cagcatatag aaatagttta attgtgcaag
    11221 aggtggttct tgatgaaggc acaacttgaa tataaaggaa tcgatcagct gatgcgacat
    11281 ctgaaaaaag cagcaacgct taatgacgtt caaaaagtcg tgaaaagtaa tactgctgaa
    11341 atgactgaac gaatgcaaaa aggtgcgcca gtggatacag gttacttacg aagatcaata
    11401 aacatgaatc ttttagaagc tggtttaact ggtattgtag gaccgacagc agattatgca
    11461 ccttatgtag aatatggcac tcgatttatg tcggcacagc cttatgttag accagcgttt
    11521 aattaccaaa aagtcaaatt tatggctgaa atgaaagcct tggtgaaatg atgattaaga
    11581 caagagatca atcaattttt gatgaacttt ttaaaatatc ccaaaacaaa cttggatata
    11641 aaacatacga ttacaaaact ttagaggatg ttggttatcc ttttgtggaa tttgagaaca
    11701 ctcagacaat ccatgaagta aataaaactg acattaaagg gtctgtgatt gtggttttat
    11761 ccgtttgggg attacagaag aaacgaaagc agttgtcaga tatggcatct gctcttttta
    11821 atgaagctag attgatagaa gccacagaag gctattattg ggctttaaat tatcaagcaa
    11881 gtggaattca agtgatggac gacacaacaa cgaatacacc gctaaaacgg gcggttgtca
    11941 cacttgaatt tagaattaga taggaggaag aacatggaag cattaaaagg tattgatgtc
    12001 attttgcttt atcgcttatt gaaaaaagaa actcaggaag ccgcttggaa aatggcattt
    12061 caaacagaac acgaaaatgg tttatcaaga gattcagact ctacagtgac aaaagatgga
    12121 aacgttcaaa gtttaagccc ggttgaatat gatttttcgg ctacttcaat agttgctaaa
    12181 ggagattctc atgtagatga aatgaaacaa gctttattaa atggggatat cattgaaatt
    12241 tgggaaatta ataaagcaga acagggaaca gatgataatg caaataagta caaagctact
    12301 tattaccaag catatgtgtc tgaatttact ccatcggctg ctgcagagga taacgttgaa
    12361 ttaagtttat catttgcagt aaatggtgtt ggtcaagatg gttatgcaac cttgacagaa
    12421 gatcaagccg atgttgttca atatgcattc aaagataccg tgaaagcaac ttcgacagga
    12481 gcataagagg gcttagatgc tctctttttt attttaggag gatgaaaaac attgaaatta
    12541 aaaattaaag gtaaagaata ttcgtttaaa tttggcacta aatttgtacg tgaattagat
    12601 aaagtgatgc ctttcgtcga tggaaatatg gaatttggaa tgggactctc cgcaaaagtc
    12661 ttaccagagc tacgttctta taacgttaac acgttatcac gagttctgga aatagcaaat
    12721 agaactgaag atgaatctat tacattagat gaattggatg attacatcga tgaagttaaa
    12781 gacattgaaa aattgtttga tgaagtccta aaagaattgg cggagtcgaa cgcgggaaag
    12841 ttagcggtcc gaaacctgaa tcagaaattg aaagaagcgg aaaaacaaca agtggaatag
    12901 attctgcact ggcatacgaa caaattctta tcaattcttt tcgatatttg ggaatgacca
    12961 atatctcaga tatcgaaaga atgacgttat atgaatacaa cattcgtatg actgcagccc
    13021 agttatcttg gcttgacaaa gaaaagttga ttcacgaatt agcgtgggca aatcagcaag
    13081 tccaagcgga gaaaaaagta ggcaaaaaga cagttcctgt atatcgatcc tttgaagaat
    13141 tcttcaatta tcaaaaaatc gaagattcaa tcatgggagt ttccgaactt tcaaaacaag
    13201 ataaaaaatt ccaaagctta ctaactaaag ctaactcttg aggaaaggag gagaatcatg
    13261 gaacaatttt ctgttgaagc cttattaaaa gccacagata gtggatttgt aaagactttt
    13321 aaagatgcac aagatgctgt taaaactttt gaaaagaatt caaatagtat gacaaccgct
    13381 gttggtaaag tgatgcaagg tactggtgcc gcaatgacaa agtatattac cactcctctt
    13441 ataggagtag gcgtagcagc tgctaaagtt ggtggcgact tcgaagcaca aatgagtcgt
    13501 gtaaaagcta tatcaggagc aaccggcgac acattcgaac agatgaaaca gcaagcgatt
    13561 gatctaggag caaaaactgc ttttagcgca aaagaatcag ctgctggaat ggaaaactta
    13621 gcttctgctg gatttagcgc acaagaaatc atgaaagcaa tgccgggtct tttagactta
    13681 gcagctgtat ctggagggga tgtggctcta gcttctgaaa atactgctac tgctttgaga
    13741 ggatttggtt tagaagcaag tgaagcagga catgtcgctg atgtatttgc tcgtgctgct
    13801 gcggatacca atgctgaagt tggagacatg ggagaagcat tgaagtatgt tgctcctgta
    13861 gctaattcaa tggggatttc tttggaagaa actgcagcag ctattggtat tatgagtgac
    13921 gcaggcatta agggttctca agcaggtaca acgttgcgag gagcattgtc taggttagca
    13981 aggccaacaa aggctatgca agatacaatg gataatttag gtgtttcgtt ttatgatgct
    14041 gacggtaaaa tgaaaccttt aaaaactcaa gtagaattac ttaaaaaagc ttttgaaggc
    14101 ctgacgcctg aacaacaaca aaatgcttta gtaacactat atgggcaaga atcattatca
    14161 gggatgatgg ctttgattga taaaggacct gattcattgg gcaaattaac aaaatctctg
    14221 aaagattctg atggtgcagc tgacgatatg gctcggacca tgcaagataa tatgaattct
    14281 tccatcgagc aaatgtttgg agcttttgag tcagcagcta ttgtaattca aaagattcta
    14341 gcaccatcca tcaaaaaagt agcagatgcc atatccggct tagtagaaaa atttgtaagt
    14401 gctccagaat caactcaaaa attagtagtg gccataggag caattgtcgc tgctatagga
    14461 ccgttaattt ttatgattgg ttcagtaatt atatggatca atagggtgaa agtagctttt
    14521 aaagctttaa gtgaaagttc aaaattgttt agtggattaa gtaaagcaat gggtcttctt
    14581 acaaatccgg tttttctggt tatagctgcg gtagcactac tcgttgtagg tttcatctat
    14641 ctttggaata cgagtgaaga ttttagaaac ttttggattg gcttatggga gggaatcaag
    14701 tctgctgtaa gctcggcagt agaatggatt cagaatgcat ggaaatctac aggagaatgg
    14761 tttaacaatt tatggaagtc cattaaagaa ggcgcagaca atgtttggac tacagttcaa
    14821 gaagcccctg ggaaagcggc agattggatt aaaaacaaat ggactggaac aaaagagttc
    14881 ttttcaaatt tatggtcaag tattgcaaac tctgcttcag agatttggaa tagtttaaaa
    14941 gaaggtgtca tatcagttat tgatgattta gtttcaagtg ctggtgaaaa atgggaaggg
    15001 tttaaaaata ctatatctac tgcatggaaa acaattacaa gtaaaatcaa atctggtttt
    15061 gattttatac taaaatatat tggtccattt gtaagtagct tttcagatgt gttctctaat
    15121 atagtgaaag caataacaag tatatttgct gaggttaaaa acataatagt aaatgcttgg
    15181 gaaatcatta agtctttaat agctgcgcca ctactgttta ttatagattt aattactggt
    15241 gacttcgaac aaatgaaaga ggatttagat ctaatctgga acacacttgt ccaatcagtg
    15301 gtaaatattt ggacatctgt aaaaaatata tttacggaat atatcggtgc aatagtaaat
    15361 agtgccgtta gtttatggac tggattcata caaagtattt ctaatatttg gaatgaagta
    15421 gtttatcaag cgactatgat ttggattgat ttgaaactat tttttactaa tttatggatt
    15481 gatattaaat acagtgcaat tcaaatgtgg ataaatctaa aattctccat aattcaaact
    15541 tggattgata caaaatatgg tgcaattgaa ctttggaata atctaaaaca atggtttttc
    15601 caaacggtta ataatatcgt gcaaactctt ataaaaagtt ggaacagctt aaagcaagga
    15661 acgatagatt tattcaacaa tacagttcaa ggtgctaaag acatttggac ttcattcaaa
    15721 tcttggattg gtgatttaat tactggaacc aaagataaca tcattcaggg atgggaaaac
    15781 ctaaaacaag gtactataga tactttcaac aatttagtaa atggtgctca agaggcatgg
    15841 gataatttag taaatgctgt tagtgacacg gttgatagag taactggctg gtttgataac
    15901 ttgaaaaata tcgatttact agcagccgga aaagctatca tggatagttt tctagaaggg
    15961 ttacaaaatg catggaaatc tgtgcaagat tttgttggag gtattggtga ttggattcgt
    16021 gaacacaaag gacctatcca atacgataga aagctattga ttccagctgg tcaggctatt
    16081 atgaacggtc tgaataaagg tctgacagga ggattcaatg acgtacagaa tactgttgga
    16141 agtatggcag actttatcgc ggaacttttc aatgcaaatt ctgatgtaga tatagctgca
    16201 aatttgaaaa atgcaaataa aaacattggt gcacaagttg aacataaaat aaatatgggt
    16261 ggctctacta aaccagctgt atttaaaatc aatcttggaa gacaatcgtt tagattgttt
    16321 atggacgata tttcacaagc tatgggcgaa ggtgcagata ttaatctgga attttaggag
    16381 ggaatatttt ggatcaacga gaaaataaaa tgtactcatt caaagataca accattaatc
    16441 tcactagttc taaacgattc cttccgacgt ctgccatgat gtacgatgga atgtatttag
    16501 aagatttgat tgatggttat caaacactta cggtggaagg tagagaaatg ctttctgtag
    16561 aagttgaaca gcaagagata caaattggtt caatcattac aaatcagaaa ataccttcaa
    16621 gaacactaaa aataacatat aagctggaag atagagatcc agaaaaacta cagtttaaat
    16681 tcaaagaact gttgaattat ttataccgga atgaagacgt ggaaattagg tttcatgatg
    16741 aattagatta ttattactac ggtcgctata catcaactga tactgttcca ggagactcca
    16801 actcgattat ttcgagtttt aatgtattct gtgcggatcc actaaagtat acgaaagagt
    16861 gtgttagtga tggctatatt ggaaatccga tacagtttcc tataacacca agaaaaattg
    16921 aagttacttt atccatgaat aattcaatca aaattacaaa cggagaacaa aatatcacga
    16981 taactgacgc ggcaataaaa acaggagacg tgttggtttt tgatttttcc gatgagcagg
    17041 taactgtaaa cggagaagat tgtacttcta tgattgattt agaaagtgat tttgagaact
    17101 tttatcttaa gcaaggtcag aagataacta gcaataatgg gaagcttaaa atattctata
    17161 ggggggcgac aatttgagtg agacagttta tttctttgat cacttgcaaa aacttattaa
    17221 aagaaaaaat acaagaagtt tgattgaagt ctcccaagaa aaagaaatta gttctgataa
    17281 gagtgatcta atgaaagata ctctttacgt tacgacaaaa tatgataaag aaatagagga
    17341 tgcaagatat atggcgattc gtgaaaacga gtcgtctttt tcgttgtatc gaattactaa
    17401 agttagcgac ccatttgaaa cattagagtt tacagggtta ggatttgcga caaatgaatt
    17461 agatgcttac atcatcaaag atattaggcc gagtgggcag cccttaaaaa atgttcttga
    17521 tcgattgatt gaatttactg aaggaaattg gcgcgttggt cacgtagaag caatgttacc
    17581 aacagtaact gcaacttttt actatgtctc tgtaaaagaa gcgttgaaag aattgcaaac
    17641 cttaggtatg gaatttgtct ttaggtgttc tttgaattct gatggaataa aggataaatg
    17701 gatcgaagta tatgaacaaa ttggcgaaga atcgaataca cgtttcgtat atggtagtaa
    17761 agcattaaca gttgtaagag agatagatag aagctcaatc tcaacttcaa tgataggtcg
    17821 tgggcgaggc gaagaggttg gtgacggtta cggtagaaga attgaattca ctgatgttga
    17881 atggaaaaag tcgaatggtg atcctttaga taagcctaaa ggccaaaatt ggcttgaaga
    17941 tccggaatca actcaaaagt atgggatacc acaaaaagat ggatcaatga gaaaacgaga
    18001 aaccgtagta gtgtttgatg atatagatga tccaacagat ttacttaaaa atacttattc
    18061 aaccttaatc gattctgcta gaccgttagt acaattcaaa gctgaagtca ctggaggaga
    18121 tgtgatagga aatacagtga ctattcacag atacgataaa ggttatcact ataaaactcg
    18181 tatttataaa actacattca atcggcttac cggtcaaacg aatatcgaac taggggataa
    18241 tttaacacaa gatgttagaa aacaaacggc ttctattgtc aataatatta atagtttaga
    18301 atctagcaaa atgacatttt acgaatcgac agagattgga aaatatcaag atgacattat
    18361 gcgaggtgca ggagataatg gcggttctat ttattgggta aatggaattg aagctggtgt
    18421 tagtgatagt agagaaatct atgaaactgt ttatatggat ggacctaaca ttcctagatc
    18481 acgctttttt atggtccaaa ataactcagg gatatctttc aaacaatgta aaaaaggtga
    18541 gtggcaaaca attcaagatg tacacaatgg tgatagcacg accgcttgga cgttggatgg
    18601 aactttcaat gctaatttta ttcgcgcagg cattctgtct ggtatcctcg tgcaaggggt
    18661 agctttaaaa acatttgacg ataaagattt ccaattagtg gcagaggggg gagaactttc
    18721 ttttgaaaaa caagtcaagt ctactggact agatgatgta catggagaac gcttgggctc
    18781 aattgtatct acttatggtt ctaaagggat taacggcttt gctgtctgga aagagccaaa
    18841 ctatattttt tccattaatg ctggggacgg tggcgatcga gggaatccta tttttcaaat
    18901 tccagcagat gttactgctg ataagcgcaa atataatctt tatggtgacg gcaatttttc
    18961 agaaggaaat ataaccatag atggacggct agatgtaaaa gaactctatg ttaacggtac
    19021 aaaaattgac actaatggag gaggcaattc cggagggaac gataacggtt ggaacggaca
    19081 atacccacca gaagtaacta ctgatcggga taaacgatat tggcaaattt gggcaacggc
    19141 aataggtgcc ggttttacac aacaagctgc tgcagcctta cttggaaatg cacaaggaga
    19201 atcagatgct aatccaaccg ctgatgaggg caatggcgca ccagggttcg gttatggtgt
    19261 atggcaatgg accgattctt ctggcgcaac tagcggacgt gtctacatga tcaatttaat
    19321 gacaaaggct ggcatcagtg atgatccaga cacaatcacg gcacagttta aattgttgat
    19381 gtggcatgca ccaaatggtc aatggatcgc aactagcgct tatccttaca catggacaca
    19441 attcatgaat ctgaccgata tcaacacagc agcacaagca ttcgtggcta actttgaacg
    19501 tccacgtgat ccacatccag aacggacggc atggtcacaa gaatggtatg acaagttcaa
    19561 agatttggaa attcctgtat caaaaggata tatgaagccg attgcggatc caatcacagt
    19621 gacgagcgaa tttggctggc gcacttctcc aatcacagga gcacaagaat ttcataacgg
    19681 tattgacctt gtaaatggaa atcctaatac acctattttt gcttcagcgg atggcgaagt
    19741 gattgttgca ggtgatgcaa attactttga ctggtatgga aattggacag tgattaaaca
    19801 cgctgatgga atgtatacag gatacgcgca tcaaagccga gtagacgtct caaaaggtca
    19861 gaaggtaact gctggtcagc aaattggact gatggggaca acaggaccat ccactggaga
    19921 acatcttcat tttcaattta tggatgagtt ttatccatct tcttcaggtc atttccacaa
    19981 tgcaagagac tacatcgatt tctaaaggag ggatagtcat ggcagaaacg cagcataaaa
    20041 tggtcctatc caccaccgaa ccaaacaacg gaataaattt ggttcgaatt cggcaagggg
    20101 atgttttaac gcaaaagttc gttgttgaag tggtggaaca tggcaaacta aaaacattcg
    20161 agggcctagt gccgtttttt attaatacaa caaaatttgg cgaaaaccaa cctgttgaac
    20221 aaaaagtaca agaatacagt ccagcgcaag caaggcttgt ttacacctta agtgagcctg
    20281 actggcaatg gggtggtgaa aacaccgcac atttcagttt ccgatcactt aatggtgatg
    20341 gaacttggag tgaacaattt agcacacagg attttaccta tcgagtcatt tctggaatat
    20401 ctagaagcca gttacgtgac tctggctatg tgtggacatt tgaggatttg ctaagaaaat
    20461 tcaaagatta catggatcag ggcaaaaatg actgggagca gtggttagaa gataatcgtg
    20521 aaatactgga aaatatcgat ccaggtggta tgatcattaa cattctgaat gaagctaaag
    20581 gcgactacga atcattagct gatcgtttaa accaaaaata ccaagtgcca gtcggcagct
    20641 cacaaattag agaaacaaca cgcttttttg attacgacac gatgaaatac gttgacctag
    20701 tgccgcgcaa tttgaatacg gttgtcaaca gtgttaataa cagcaaattt aacttttctt
    20761 tcattacaga cattcacgca gataatcaca acttgcgtat agatggcgtc ggttacaaag
    20821 atgcttctta tttaagacat tggcgcgcaa tccctcaatt tcaaaaatta gggaacaaaa
    20881 cagatgtgat gatttacggc ggagacaata tcgacggcgg acttggttca ctcggcagcg
    20941 atataggcat tattgatgaa tggagcgcgc gacattccat gttaggcacg ctcaaacgct
    21001 tcactaacgc agcggtagca ggacaagaaa aaccggttat tatctgcaaa gggaatcacg
    21061 acgcttgttt cgaccccgcg tggcgcaagc gaaagggaat gttatgcaac gctgacttcg
    21121 aagagtattg gaacggtttg tacggtggcg tgttgttccc agataaaaac gcagcaattt
    21181 accgtttcga tacttgtgat ttttatgaag gcggcacagg cgacaagtac gtggacggtt
    21241 acagcgatac agcgccggga gctttcagtg ccaaacaaat taacgctttc ggagaatggt
    21301 tagtgaacgt tccgagaaac tatcatgtgg cgttagtagg acatacgcct ttaggtctca
    21361 gcaagtttcc cgttcgcaat gaaaacatga ttagcacgtt gatagaaggg tttaaatctg
    21421 gtagtccggt aactatcgat tggtctaaat taggacaacc gaatgacggt tcgttcggcg
    21481 gattaaagac cttcgcaatg aatacaaaag gcgccggcgt tgtagtaggt tatttctgcg
    21541 ggcattggca cgaacaagtc gaaggggcgt ttggaacagt aaaaatgatt ctttgcgacg
    21601 tcggcttctc tcatacagct agccaagttg atacaccgga tgaattagcg ttttataaaa
    21661 tagaagttga cacagcaaca agaaaagtga caagcaaagg cgtagggcgc gcgagagact
    21721 tcacttataa ctattaaggc ggtgaacaat taaatgttag attttcaaag caaaccaaat
    21781 atttttgaag aaatgagtta tgaagaggct gtgaaatggt tattgcgtca agcggctatc
    21841 cattacgacg gctcggatca cgacgcgcac gtcctagcta ctgaaagcaa cgcgggcttt
    21901 gctacaccgg aaacagttat gcaagcgcgt gggcgttggt tacgcgatta taagctacca
    21961 caaaaatatc cgaacatttt agacattccg cccggcaaat acgcaaccaa agccggatgg
    22021 ggcgcagaca accctgacgg gattgaagat gacagttttg ttgaaatgat ggtattcgcg
    22081 gatcacgatt cgagaaaatt aatcgtcgct tttgctcgtt atagcggcga aatttacatc
    22141 aaaatgacac ataacagcga accggtagag ggttataact cgttaggctg gcggcgcgtt
    22201 tacactactt ctgttctttt tgaaggcgaa ttaagaaaag gacaatcagt caatctgcca
    22261 gatgatactt tccgttacca aacgcttcgt atccactaca cagacggtga cggagacttt
    22321 gtagaagaag tgaaacgtca aagatacgcg cgaattacaa aagcgaattt atggaatgga
    22381 gacgcgggag tgacgttcat cgaatttgag ctgacaatcg aagcgcgaaa gataacgatg
    22441 tcaaacggca gagctttaga tatttcatcc ggcaatgttt ccaatcccgc aatgagcaac
    22501 aacgtgaaaa taactaagat tgaaggtgtg aaataatggc gcatgtcatc aaaaaaggct
    22561 ctatcaaagt acctacacag ccgaaagact acgatttgca agcaacgggg cttgtattta
    22621 aatcatacga taatcaaata gcgttagagt tcaacgtcga acaacagaac ggcacaccgg
    22681 cggacttgct aggagctaac ttgcgcttgt tgatgtttat ctatgatgaa gtagatggaa
    22741 tgatcacgaa agagccaatc ccttttatca cgaaaaacct catcactgaa agcttcttga
    22801 atggacaggt cgtatatatc ttgccagaag cgatgaaagc ttataacggt atggtggaag
    22861 cttatgttta catcgagtat ccagacggat caacaagtga taacttaggc ttcaccttcc
    22921 gtatgaagcg ttcagcaatc gacggactag cgcaagataa agcagactac tttattgcag
    22981 actttcaaca attacttgat ggagtcaaac aagaagcaac agatgctgtt aacgcagcat
    23041 taacaaatgt ggattctacg attgaaaagg caaaccaaca aataaatgaa tttgtagagg
    23101 gagccacaca agcaattgat caaactgttg acgaggtaac agagcaacta caagctactc
    23161 aaactaagat tgataccgtt tctcaaaacg ttacatcggc acaaaacaat cttaaagcag
    23221 ttgaagacaa gatgaatcaa accaatcagc aaatcggcga cctcggcaag ctgaaaaaga
    23281 tgtacagtaa cagcatcgac ttcgggaatt atgattatag tgggaatccg aatttaatgt
    23341 ccaaactaaa atcgagcgat tttaacgttg gttaccacgg gtcactaact tcggataacg
    23401 aaaagctaca ttttacttct gatggtacag gaagcattat tatgtttacg cgtattaata
    23461 cacctcagct tgctagtggg aaaacctata ctctgagtgc gaaagttcga tttgatgaag
    23521 gaactacagg agctattgat aaattacgtt tggtgtatcg tacatcacca ggagaaaaga
    23581 tattattgga agcaaatagt acaaatatta caacagatga tgtagggaaa gaaataacaa
    23641 tcaaaggtac agctaacgtt aattatcaaa tcacaaattt agatcgattt tatatgagta
    23701 ttagctttgt tgacagggat aaaataaatg gcggatttaa gttgtacgac atcaaaat
//
LOCUS       DACCYH010000039        23653 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.039, whole genome shotgun sequence.
ACCESSION   DACCYH010000039 DACCYH010000000
VERSION     DACCYH010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.039"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..506)
                     /locus_tag="IVY85_001491"
     CDS             complement(<1..506)
                     /locus_tag="IVY85_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8044516.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSM"
     gene            779..3250
                     /locus_tag="IVY85_001492"
     CDS             779..3250
                     /locus_tag="IVY85_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8044517.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3353..5089)
                     /locus_tag="IVY85_001493"
     CDS             complement(3353..5089)
                     /locus_tag="IVY85_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP8044518.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5086..5790)
                     /locus_tag="IVY85_001494"
     CDS             complement(5086..5790)
                     /locus_tag="IVY85_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8044519.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5921..6424
                     /locus_tag="IVY85_001495"
     CDS             5921..6424
                     /locus_tag="IVY85_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP8044520.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6384..6722
                     /locus_tag="IVY85_001496"
     CDS             6384..6722
                     /locus_tag="IVY85_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP8044521.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6743..8161
                     /gene="ffh"
                     /locus_tag="IVY85_001497"
     CDS             6743..8161
                     /gene="ffh"
                     /locus_tag="IVY85_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP8044522.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8273..8851
                     /locus_tag="IVY85_001498"
     CDS             8273..8851
                     /locus_tag="IVY85_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP8044523.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8855..9376
                     /locus_tag="IVY85_001499"
     CDS             8855..9376
                     /locus_tag="IVY85_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8044524.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9455..10009)
                     /locus_tag="IVY85_001500"
     CDS             complement(9455..10009)
                     /locus_tag="IVY85_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8044525.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10262..10537
                     /gene="rpsP"
                     /locus_tag="IVY85_001501"
     CDS             10262..10537
                     /gene="rpsP"
                     /locus_tag="IVY85_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP8044526.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10549..10800
                     /locus_tag="IVY85_001502"
     CDS             10549..10800
                     /locus_tag="IVY85_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8044527.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10925..11716
                     /locus_tag="IVY85_001503"
     CDS             10925..11716
                     /locus_tag="IVY85_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044528.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11886..12407
                     /gene="rimM"
                     /locus_tag="IVY85_001504"
     CDS             11886..12407
                     /gene="rimM"
                     /locus_tag="IVY85_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP8044529.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12397..13158
                     /gene="trmD"
                     /locus_tag="IVY85_001505"
     CDS             12397..13158
                     /gene="trmD"
                     /locus_tag="IVY85_001505"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP8044530.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13294..13641
                     /gene="rplS"
                     /locus_tag="IVY85_001506"
     CDS             13294..13641
                     /gene="rplS"
                     /locus_tag="IVY85_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP8044531.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            14014..14676
                     /locus_tag="IVY85_001507"
     CDS             14014..14676
                     /locus_tag="IVY85_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044532.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            14755..17982
                     /gene="fss3"
                     /locus_tag="IVY85_001508"
     CDS             14755..17982
                     /gene="fss3"
                     /locus_tag="IVY85_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP8044533.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            18096..18410
                     /locus_tag="IVY85_001509"
     CDS             18096..18410
                     /locus_tag="IVY85_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8044534.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            18423..18797
                     /locus_tag="IVY85_001510"
     CDS             18423..18797
                     /locus_tag="IVY85_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8044535.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            18807..19151
                     /locus_tag="IVY85_001511"
     CDS             18807..19151
                     /locus_tag="IVY85_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP8044536.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            19222..20574
                     /locus_tag="IVY85_001512"
     CDS             19222..20574
                     /locus_tag="IVY85_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8044537.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            20634..21776
                     /locus_tag="IVY85_001513"
     CDS             20634..21776
                     /locus_tag="IVY85_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAP8044538.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            21801..22055
                     /locus_tag="IVY85_001514"
     CDS             21801..22055
                     /locus_tag="IVY85_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044539.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            22311..23495
                     /locus_tag="IVY85_001515"
     CDS             22311..23495
                     /locus_tag="IVY85_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8044540.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            23510..23629
                     /locus_tag="IVY85_001516"
     CDS             23510..23629
                     /locus_tag="IVY85_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP8044541.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
BASE COUNT         7832 a         4218 c         5073 g         6530 t
ORIGIN      
        1 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
       61 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      121 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcac ctgcttcagt
      181 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      241 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      301 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      361 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      421 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      481 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      541 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      601 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat
      661 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
      721 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
      781 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
      841 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
      901 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
      961 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     1021 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     1081 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     1141 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     1201 tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt
     1261 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     1321 caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     1381 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     1441 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     1501 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     1561 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     1621 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     1681 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     1741 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc
     1801 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     1861 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     1921 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     1981 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     2041 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     2101 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     2161 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     2221 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     2281 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     2341 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     2401 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     2461 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     2521 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     2581 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     2641 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     2701 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     2761 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     2821 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     2881 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     2941 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     3001 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     3061 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     3121 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     3181 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     3241 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     3301 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     3361 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     3421 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     3481 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     3541 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     3601 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatt agatttttga
     3661 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     3721 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     3781 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     3841 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     3901 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     3961 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     4021 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     4081 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     4141 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     4201 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     4261 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     4321 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     4381 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     4441 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     4501 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     4561 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     4621 aaaaaccgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     4681 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     4741 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     4801 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     4861 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     4921 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     4981 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     5041 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     5101 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     5161 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     5221 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     5281 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     5341 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     5401 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     5461 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     5521 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     5581 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     5641 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     5701 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     5761 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     5821 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     5881 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     5941 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     6001 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     6061 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     6121 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     6181 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     6241 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc
     6301 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     6361 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     6421 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     6481 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     6541 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt
     6601 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     6661 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     6721 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     6781 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     6841 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     6901 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     6961 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     7021 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     7081 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     7141 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     7201 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     7261 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     7321 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     7381 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     7441 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     7501 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     7561 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     7621 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga
     7681 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     7741 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     7801 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     7861 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
     7921 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
     7981 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
     8041 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
     8101 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
     8161 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
     8221 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
     8281 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
     8341 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
     8401 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
     8461 tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
     8521 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
     8581 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
     8641 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
     8701 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
     8761 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
     8821 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
     8881 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
     8941 tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat
     9001 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
     9061 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
     9121 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
     9181 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
     9241 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
     9301 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
     9361 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
     9421 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
     9481 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
     9541 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
     9601 cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac
     9661 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
     9721 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
     9781 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
     9841 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
     9901 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
     9961 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
    10021 tccttcaaac tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac
    10081 ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa
    10141 atcttctatt ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca
    10201 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
    10261 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
    10321 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
    10381 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
    10441 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
    10501 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
    10561 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
    10621 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
    10681 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
    10741 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
    10801 ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttta ttaattgaag
    10861 aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca
    10921 gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc
    10981 ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag
    11041 tccagaaaaa agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa
    11101 taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc
    11161 tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg
    11221 ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa
    11281 tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac
    11341 aatccaagtt ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc
    11401 tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg
    11461 aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc
    11521 taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa
    11581 aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc
    11641 gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc
    11701 tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca
    11761 ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag
    11821 aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat
    11881 ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg
    11941 aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc
    12001 taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac
    12061 ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat
    12121 atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat
    12181 tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag
    12241 taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag
    12301 ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag
    12361 tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta
    12421 tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa
    12481 ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca
    12541 gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat
    12601 gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt
    12661 gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac
    12721 ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca
    12781 gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg
    12841 atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg
    12901 gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa
    12961 gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa
    13021 ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa
    13081 tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca
    13141 gcagatgaag ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta
    13201 gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct
    13261 cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca
    13321 aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac
    13381 gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa
    13441 cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc
    13501 gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac
    13561 ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt
    13621 atcaaagaaa tcagaagata aacacggaaa acttccttgg aaatctgaaa tttgtgaaaa
    13681 gtccttgtat atcaagggct ttttcttttt tctctatcac agttttaagc catttttggc
    13741 tgttttggac catttttcaa tcatattccc ttaaaaattg tcctagattg tccgacattg
    13801 actatcagtg tcgtcaaaat agggcaaaat ttagtagtca gcttgacgac aaatataata
    13861 cgaaaaatac caaaacattt accgaattta gcaacttttg aacccaagtt tctcaaaggg
    13921 tcagctatta gcaaaaaaga caaaacatgt acttttctac taaaaagttg atgtgatagg
    13981 cttctatcta aataactatt aggagttttt atcgtgaagc aaaatgatgg acgtatcatt
    14041 tggaaaaatc aaagtgagtt aaaactgatt ttaacaatta atgaattcat cgaaaaacac
    14101 ggaattacat ctagccgtca ataccagaaa aagctatcag aaaatccaaa ttctgcacca
    14161 agtatgtggt tcattaacaa aaaatatgga tcatgggaga atttattgat tagtatagga
    14221 cgagaaaata ctgggtatgg aaaatgggct agaatgtcag aacaagaatt attggaaata
    14281 gtagaagcct ttattaagtg cgagaagata acctctcaac gaatgtacga acaaaaatca
    14341 gtagggaaga atattccttc acttagtacc attaaaaaga tgttaggaga tatacgtcca
    14401 ctttttaaag aaaaaaatga tggatctcga tttactgatt tcgaactgct gttagaacta
    14461 aaaaatgaaa taatccgatt aaaactacag gatgatttat caatgacaaa gtttcgaaaa
    14521 ctagttcaat ccccaaaact accatcagtt gatacaatta tgaaaagaac gaacaaaaat
    14581 tgggaggaat tgatggcaga gattggattt gattatcgaa gaatcaaaat ttataagcag
    14641 agaaacaacc tatccaagac aaagaaaact aaatagtatc actctaaatc agacgttcaa
    14701 aattaaaatt tggacgtctt ttttattacc caaaaacgaa aggagattta ttttatgaaa
    14761 aagccaagat ttaagaattg gcgtttgctc gcaacactga gtctattatg ccaaacaatt
    14821 ggtggatcgc ttggcccaac gattgccttt gctgatgaaa ttactcatcc acaaacggta
    14881 actgtggaat tggatttggc tcaccagtat gctgtcgaag gtacatttag cgatggtcgt
    14941 cccatgtcag aagttaccgt tccgcactat gcagtttaca atggtgtgaa gcaagatatt
    15001 ttctgtattg agccaggggt gccgatttac aatgagttca ctcctggtta tgagaaaaat
    15061 ccattgcctg atatgtcgga gaaagcgaaa ttagtttccg ttttatggaa ggaagctggt
    15121 acggatgtgg atacacatat tgtggcccag aagatgattt ggcaagaagt taatggctac
    15181 acgcttcact caattaaacg ttctgatggt agtgcagtaa atatcgcagc cattgagtca
    15241 aaaatcaatc aagccattgc ggattatcaa aagaaaccaa gcttccataa ctccacagtg
    15301 aaaaccgtgt taggtcaatc gactactgtg acagatacga acggcttgaa tttatcggag
    15361 tttgatgaag ttgtcgagaa tatcgcaaat attgattatc gtgtgaacgg caatcaacta
    15421 gtgattactc caaatgccaa ttcaaaagag agtggtgaat taaccctaaa gaagtcagct
    15481 aatacaggaa caccggtagc ttataaaatg gctggtcaac aaactttgat ggcgggggca
    15541 attgataagc ccaataccta cacaatcaaa attgatgtgg aaactgaggg ttctttgaag
    15601 attaaaaaag tcgataaaga aacaggaaat gttgtaccgg gaaccgtttt ccatttagac
    15661 tttggaaaaa cattacctgc aaaagacgtg accaccgata aagaaggcat tgcgacattg
    15721 gatgagattc ctcatggcgc gaaagtaacc attactgaaa aatcagtgcc agctccttat
    15781 acgattgaca ctacaccaat caccgcaacc attaaatctg gtgagactat ttctgtgact
    15841 tcaaaaaata ctcgcgaaaa aggtcaaatc attttgaata aaatgggggt ggaaacagga
    15901 actgatcttt ggaacgacaa atactcatta gccggaaata cgtttgccat tcgcaaagac
    15961 agtccaaccg gtgaaattgt ccaagaaatg accacggatg aaaaaggtca tgcggaaaca
    16021 ccaaaagaga ttgccaatgc gttggaattg ggaacctatt atgtgaccga aaccaaagct
    16081 agtaatggct ttgtgaatac ctttaaacca gtaaaagtcg aattgaacta tgtcaatcaa
    16141 accgtggctc ttgttaccag taacgtaaaa gggcaaaacc aagaaatcac tggtgaaacc
    16201 actttgacaa aagaagacaa agataccggt aatgaaactc aaggtaaagc tgagtttaaa
    16261 ggagctgaat atactctctt tactgcaaaa gatggtcaag cggtcaaatg gagtgaggct
    16321 tttaaaccag agttagtgaa gggaacgaaa gcttctgatg aaacggtgac tttgacttta
    16381 gatgaaaaga atcaagttgc tgttaaacac ttagcgatta atgagtatta ctggcaagaa
    16441 accaaagcac ctgaaggata taccttggat gaaacaaagt accctgtttc catcaaaaag
    16501 gtggatgata acgaaaaaaa tgccgtgatt acccgagatg ttacggcaaa agaacaagtc
    16561 attcgctttg gctttgattt ctttaaattt gctggatcag ctgctgggac tgccgaaact
    16621 ggcttcaatg atctgacctt taaagtatcg ccattggaag gaaccagtga gattacaggt
    16681 gccgaagatg aagcaattac agcttataac gagcaactag gttttgatgg ctatggcaag
    16741 tttgaaaatc ttccttatgg ggattattta cttgaagaag tagaggctcc agaaggattt
    16801 caaaaaatta cgccattaga aatccgttct acttttaaag aaaataaagc ggattacact
    16861 aagagtgaat acatttttac gattaccgaa aaaggacaaa aacaaccaat taaaacggtg
    16921 accgttcctt acgagaaact gacgaataaa gcattttctg ttagcttgaa tcgtttgatg
    16981 ctctatgatt tacctgaaga agaagatagc ttaacttctc ttgggacttg gaaagacgga
    17041 ggcaaagaat tgacagacct tgattctacc gagctagttg ataaattgag ctataacttg
    17101 catgaaatca aagaagactg gtatgtggta gcgcaagcca ttgatgtgga agcgacaaaa
    17161 gctgcccaag aaaaagatga aaaagccaaa ccagtggtaa ttgccgaaac aaccgcaacg
    17221 ttggcgaata aagagaaaac aggaacttgg aaaattttgc ataaattaac cgcagaacaa
    17281 gttttggata aaaccatcgt cttattcaac tatgtgtatg aaaacaagaa accctatgaa
    17341 gcaggtgatg atccagtagc gaaagatgct agcttgaaca atcaagccca aactgtcaat
    17401 tgtacggtgg aacgccatgt ttccatccaa acaaaagccc acctagaaga tggttcgcaa
    17461 acctttactc atggggatgt ggtggatatg ttcgatgatg tgtcgattac ccatgatgta
    17521 ctggatggct caaaagaagc ttttgaaaca attctgtatg cattacttcc ggataatacg
    17581 accaaagaaa tttggaaatc tggcaaaatt gattatgaag tgaatgacaa agaattcact
    17641 aaaaccgtac ttgcggaaaa agtggacaca agtaaatatc cagaaggtac aagttttacc
    17701 ttcaaagaaa ttaactatgg caaagacgga aacatcaatg ggaaacacaa tgaggatcta
    17761 aaagaaaaaa ctcaaacttt aacacctaaa aaagttccga caacaccaag tgcaccagaa
    17821 caaccggaaa taccaactgt tccaagtgac tctcaagaat ctagtcctac agtaaagaca
    17881 ttcccacaaa caggggagaa aaattctaat gttctgctgt ttattgggtt taccttgatc
    17941 tttgcgactg ctgggtatta tttctggaat cgccgaaact aaggtgatgt gatgaaacaa
    18001 ctaaatagga aaaaccaacg tgcgcgaccg cctcctaaaa aaagtggtcg cgctattttt
    18061 gtatccaaaa ctaaaaatga aaagaggaaa agaagatgga attgaaattt gttgtgccag
    18121 atatggccga aacctttgga aagataaggt atgccggcga aggcgaagtt ttaactgaag
    18181 gatatgggcg gaatactaca gtaattggtc gtagttatca tttgtattcc agtaagcaac
    18241 gagccgatga tattgaagtg gtggtagctg cggaagccgg cgaaaaggat tttgatcaag
    18301 atcaaccact aaaagccgtg aatccccatt tggttgccaa aggctatgag attgagaatc
    18361 gtgggtttac cgactacgtg ttgtatgtcg atgatttagt gaaagcatag gaggaaaaga
    18421 agatgagatt aacagaaggt attgttgtag attcaggact aacgtttggg aaattacgat
    18481 tttccgcatt acgccgagaa gtacggaagc aaaatgagga tggaacggtt agtaacgagg
    18541 taaaagaacg gacttataat ttgaaatcct ctgcgcaagg tcgaatgatc caagttagta
    18601 tcccagcaaa tgtaccctta cgcgagtttg cctatgacgc agaggtagag ttggtcaatc
    18661 caatcatgga tacggtcgcc aattatgttt tccgagaagg aactactgtc aattggttca
    18721 ttaaggccga tgatttagtc ttgaaacgac aaccagatca gggaaattct acaaaccaaa
    18781 atgaaggaaa gaaataggtg aagcgtatgg aaattaatgt tgaacatatt ttagattgtc
    18841 tcgatcaata tggaaaaggt gagctaacgg aggaacaact cactaaagca ttaacctatg
    18901 atgaaaaaat gtttctgatt atgcatcaag ggttactgga agtaggtaac gatgtcgaag
    18961 aagattttga tgtactgaat tggttagggg aagaagagtc cttctttatg gttatcgaag
    19021 taaatgaaag tctatgtcga gaagccgaaa ccgtattgga agaaattggt gtggaaatgc
    19081 cggatgccat tgaagttttt ttaaagcaac tagtggaaac gaaacaactc cctatagcag
    19141 taaatgacta gcataactgc ttgaaattgg atcaatgaaa tcctatgaag cttttttcga
    19201 ttggaggtgg aaaccataaa aatgtacaaa gggcatcgta tacgggcagg agatcagcac
    19261 ttggtttatc actttgttct tggctggcta ctcgctctgt tcatcggttg gatgagcgtc
    19321 ttttattttc aagagcttag acaatttgat atttctaagc tatcactttc tactatagaa
    19381 atcgttcgtt ctataaaaga tttaatctat ttgctaggaa gtctcgttct ttcaggaagc
    19441 acgatgttgc tttatataca cttttttcag gatcattggc gcagtttgtg gcatcgacaa
    19501 aagctagccc ggatgattct agaaaatcat tggtacgaag taaaacaaac tcagagtgaa
    19561 ggatttttca aagacctcaa tagtagtcga accaaagaga caatcagtta cttccccaaa
    19621 atctattacc gtatgaagga tggtttactt tctattcgcg ttcaaatttc actgggaaaa
    19681 tatcaagatc agcttttaaa gctggaaaag aagttagaaa gtggtctata ttgtgaacta
    19741 gtagaaaagg aactcaaaga ctcctatgtg gagtacactt tactgtatga tataattgcc
    19801 aatcgaattg ggatagacga agtagtggca gaaagtgggg ctttgcgatt gatgaaaaat
    19861 caagtatggg cttatgattc tttacctcat atgttaatcg ctggcggcac aggtggtggg
    19921 aagacttact ttttactcac tatcatcgag gctttattaa aatctgatgc cgagcttttt
    19981 gttcttgatc ccaaaaatgc ggacttagcc gatttaggta cggtaatgcc tcatgtctat
    20041 tcccaaaagg aggaaatttc tgcttgtgtg gaagatttct atgagcgcat gatgactcgt
    20101 agcaaggcaa tgaaagaaat gcccaactac aaaacaggag agaattatgc gtttcttgga
    20161 cttccaccaa actttttaat ctttgatgaa tacgtggttt atatggaaat gttaacgacg
    20221 aaggaaagtg cggtgatttt gaataagctc aaacaaatcg tgatgttagg tcgtcaatct
    20281 ggtttctttc tgattctagc ttgtcaaaga ccggatgcga aatatttagg agacgggatt
    20341 cgcgatcaat ttaatttccg ggtggctttg ggtcgtatga gtgaactggg atattccatg
    20401 atgtttggcg aggttgataa aaaattcttt atgaagcgta tcaaaggtcg aggctatgta
    20461 gatacgggag ggagtgtgat tagtgagttt tatacgccac ttgtaccaaa aggatatgat
    20521 ttcttaaggg aaattagcga cattggtgga ttaaacgtta atacggaagg ataaaataat
    20581 agtatgtagc ctaggagtgt caagaatcct gggctttttt tacgaggtga tttatgacat
    20641 ttttagattt attcgctggg attggtggct tttgcttagg tatggaacaa gcgggtcacc
    20701 agtgtatcgg cttttgtgaa attgatgatt ttgctcgcca gagttataag gcaatccatg
    20761 atacaagtaa ggaggtggaa atgcatgaca tcacaagtgt atcagacgag tttattcaat
    20821 ctctcggacc agtggatatc ctttgcggtg gatttccgtg ccaagctttt tcaattgcgg
    20881 gaaagcggca aggattttcc gatactcgag gtactttatt ctttgaaatc gctcggttcg
    20941 ccgctattct ccaacctaag tttttattcc ttgagaacgt ccggggatta ctcaatcacg
    21001 aaggaggggc tacgttcgag acgatcctcc gaacgctgga tggattgggg tatgatgtgg
    21061 aatggcaagt gcttaactca aaggcctacg ttccccaaaa ccgtgagcgg atcttcctta
    21121 tcggacattc tcgagacgca tgtaccgaac aagtatttcc tatcattgga tcgtctccaa
    21181 catctgatca aaacatcaga aacttattaa atatcaaccc ttccaatcga ggaatggggg
    21241 gacaagtcta tgggtcagat gacgtagcgc ctaccttgac cggtgatgaa ggaatcaaaa
    21301 tcgcattgcc ggtagatgat ggaatttctg tagcgggtat gttactggga aattttgaac
    21361 aaggcaatcg ggtttatgag ataactggaa cagcgcctac tttgtcaacg aaacaaggcg
    21421 gaacgaagat catgcttcgg aaaaacgaaa cagaatatca agaggtgaag ccagggaaca
    21481 gtgtgaacct ggcttttcct aattccacaa gtcgtcgagg ccgtctggga aaacaaagtg
    21541 tgcatacctt attaacagga gaccaacaag cagtcgtcac ggatcagtat caaattcgaa
    21601 agttaacacc tagggagtgc tggcggcttc agggctttcc ggactgggct tttgatcgag
    21661 cttctcaagt caattcagat agtcagttgt ataagcaggc tggaaattct gtaacagttc
    21721 cggtcatttt tgatattgct agaagattaa aggaggtaaa aacagatgat ttatgatgag
    21781 ttgattgggg aatctactgg gtgattggca agattcagtc agatccggaa ttggaagaag
    21841 aactgtaccg gctcaatttt gatattcgca aaaacggggt gaaagtccct gatgatccgt
    21901 atgtaattga tgaggaaacg gatgctcgaa tggagctgaa tcaagtcatc agtgaattgg
    21961 aacgaattgc ggactttgct aaagaacctg atatccgcca atacttgttt gagataaaag
    22021 cagagttgga aattgagggt attaccgcgg agtaacggcc aaataaacca ctgataggca
    22081 ctgaccgcgg gggatttgcg aagcagaaat cctccgtggt gctgtgcctg tttatggacg
    22141 gcgttagccg gccatgatgt ctcacccccc gtatctaaca ggggggtaca aaaacacaaa
    22201 aaataagcaa gcaacgataa ggaatggctg tcctatcaag gataataagc aatttgtgta
    22261 gatgttaact gatttgaaaa agttaacacc ttaaatttgg aggtgagaac ttggcacaaa
    22321 ggaatttaga ttatcggcta ttaaaagacc ggcgtaatga atatggcgtt tcacaaaata
    22381 aattagctac gacttgtgga cttagtcgcc cgtacttaaa tcagatagaa aatggtggcg
    22441 taaccgcatc gacaaaaacc atgaggaaaa tttttgacca actggaaagt ttcaatcccg
    22501 agttaccttt aacgttactg tttgactatg taaggattcg ctttcccaca acggatgcga
    22561 ggaaaatcat tcaagagatt ctccatttga aatttgatta tatgctccac gaagattacg
    22621 ccttttactc ctatcaagaa caatatgtta tgggagatat tgtggtgatg ttgtcacatg
    22681 aagaagataa aggcgtcctt ttagaattaa agggtcgtgg ttgtcggcag tttgaaactt
    22741 ttttactcgc ccaaaagcgt agctggtatg actttttcga agattgtcta aaagccggtg
    22801 gcgtgatgaa acgcttagac ctggcaatca atgatcgagt tggcctgtta gatattcctg
    22861 atttaacgaa aaaatgtcag aaggaagaat gtatctcctt gtttcgtacc ttcaaaagct
    22921 atcgttccgg ggaactgttg aaacctgatg ataaagatgg tatgggcaat actttgtata
    22981 ttggcagtct taagagtgag gtctatttct gtttgtacga aaaagactat gagcaatata
    23041 tcaagttagg gattccttta gaccagaccg aaacgaaaaa tcgttttgaa atccgactga
    23101 aaaatgatcg agcttatcat gcaattcaag atttgttaaa aggtcgcagt attgaaagta
    23161 ccacattttc cattattaac cggtacttgc gatttgcgga taaggtggaa ggcaaaaggc
    23221 gaactaactg gcccttgaat gaacaatggg ggcgcttcat tggacggaat cagaaggaaa
    23281 ttcaattaac ttctgaaccg aagccttata ccattgaacg aacgttgaac tggcttggac
    23341 gacaagtcgc ccctacgtgg aagatggcca aggagttgga tcggttgaat cagacaacgt
    23401 acattcagga tatggtaaat aatactcgac tatcagaccg gcacaaaaag attttggagc
    23461 aacaaagtat ggcaattgaa aatttgatta tatgaaaaga ggaatcgtga tggaaattgt
    23521 gaaagatatt ttattggtgt ttggtggcag tatgcttggt gttactgtga tgtgcttgat
    23581 gcatgcaagt gcagcggcgg accgagcaat ggaaaagaaa ggaaagtgat gaaatgaatt
    23641 tcggtcaaaa ttt
//
LOCUS       DACCYH010000040        23648 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.040, whole genome shotgun sequence.
ACCESSION   DACCYH010000040 DACCYH010000000
VERSION     DACCYH010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.040"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(26..601)
                     /locus_tag="IVY85_001517"
     CDS             complement(26..601)
                     /locus_tag="IVY85_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8044542.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(606..1580)
                     /locus_tag="IVY85_001518"
     CDS             complement(606..1580)
                     /locus_tag="IVY85_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleosidase"
                     /protein_id="HAP8044543.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1604..1648)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1863..2831
                     /locus_tag="IVY85_001519"
     CDS             1863..2831
                     /locus_tag="IVY85_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8044544.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2928..3221
                     /locus_tag="IVY85_001520"
     CDS             2928..3221
                     /locus_tag="IVY85_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8044545.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3339..3575
                     /gene="secG"
                     /locus_tag="IVY85_001521"
     CDS             3339..3575
                     /gene="secG"
                     /locus_tag="IVY85_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP8044546.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3687..4433
                     /locus_tag="IVY85_001522"
     CDS             3687..4433
                     /locus_tag="IVY85_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8044547.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4436..6793
                     /gene="rnr"
                     /locus_tag="IVY85_001523"
     CDS             4436..6793
                     /gene="rnr"
                     /locus_tag="IVY85_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP8044548.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6815..7279
                     /gene="smpB"
                     /locus_tag="IVY85_001524"
     CDS             6815..7279
                     /gene="smpB"
                     /locus_tag="IVY85_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP8044549.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7581..8300
                     /gene="atpB"
                     /locus_tag="IVY85_001525"
     CDS             7581..8300
                     /gene="atpB"
                     /locus_tag="IVY85_001525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP8044550.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8353..8568
                     /gene="atpE"
                     /locus_tag="IVY85_001526"
     CDS             8353..8568
                     /gene="atpE"
                     /locus_tag="IVY85_001526"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP8044551.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8659..9183
                     /gene="atpF"
                     /locus_tag="IVY85_001527"
     CDS             8659..9183
                     /gene="atpF"
                     /locus_tag="IVY85_001527"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP8044552.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9170..9712
                     /locus_tag="IVY85_001528"
     CDS             9170..9712
                     /locus_tag="IVY85_001528"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP8044553.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9735..11291
                     /locus_tag="IVY85_001529"
     CDS             9735..11291
                     /locus_tag="IVY85_001529"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP8044554.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11305..12207
                     /locus_tag="IVY85_001530"
     CDS             11305..12207
                     /locus_tag="IVY85_001530"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP8044555.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12230..13636
                     /gene="atpD"
                     /locus_tag="IVY85_001531"
     CDS             12230..13636
                     /gene="atpD"
                     /locus_tag="IVY85_001531"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP8044556.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13650..14072
                     /locus_tag="IVY85_001532"
     CDS             13650..14072
                     /locus_tag="IVY85_001532"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP8044557.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            14541..14771
                     /locus_tag="IVY85_001533"
     CDS             14541..14771
                     /locus_tag="IVY85_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP8044558.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            14868..16169
                     /gene="murA"
                     /locus_tag="IVY85_001534"
     CDS             14868..16169
                     /gene="murA"
                     /locus_tag="IVY85_001534"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8044559.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            16182..16358
                     /locus_tag="IVY85_001535"
     CDS             16182..16358
                     /locus_tag="IVY85_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8044560.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            16493..16789
                     /gene="yidD"
                     /locus_tag="IVY85_001536"
     CDS             16493..16789
                     /gene="yidD"
                     /locus_tag="IVY85_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP8044561.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            16853..17320
                     /locus_tag="IVY85_001537"
     CDS             16853..17320
                     /locus_tag="IVY85_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8044562.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            17405..18289
                     /locus_tag="IVY85_001538"
     CDS             17405..18289
                     /locus_tag="IVY85_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8044563.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            18406..18639
                     /locus_tag="IVY85_001539"
     CDS             18406..18639
                     /locus_tag="IVY85_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP8044564.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            18727..18807
                     /locus_tag="IVY85_001540"
     tRNA            18727..18807
                     /locus_tag="IVY85_001540"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18761..18763,aa:Tyr,seq:gta)
     gene            18814..18885
                     /locus_tag="IVY85_001541"
     tRNA            18814..18885
                     /locus_tag="IVY85_001541"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18846..18848,aa:Gln,seq:ttg)
     gene            19401..20108
                     /locus_tag="IVY85_001542"
     CDS             19401..20108
                     /locus_tag="IVY85_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8044565.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(20167..20811)
                     /locus_tag="IVY85_001543"
     CDS             complement(20167..20811)
                     /locus_tag="IVY85_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP8044566.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            20938..21225
                     /locus_tag="IVY85_001544"
     CDS             20938..21225
                     /locus_tag="IVY85_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044567.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            21333..21653
                     /locus_tag="IVY85_001545"
     CDS             21333..21653
                     /locus_tag="IVY85_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8044568.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            21971..23047
                     /locus_tag="IVY85_001546"
     CDS             21971..23047
                     /locus_tag="IVY85_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8044569.1"
                     /translation="MFDQPEAKPREVVFFWTGQLTEKQTEIAKRILCHSEKRHHLIWA
                     VTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNELFLRYRYVFPEEKITLLHGNTA
                     TAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYNGDAGLAHAVQTAIRSNGRFIY
                     LSATPDEKLLKEIKQTFELHKLALRFHRRLLPEPVLVFMNNWRQKCGNKKKINSLVRI
                     IQQLIKENRILLFCPSITMMHRLKITLQEAFPEYEITDVSSQDDQRAKKVQKMREAIY
                     EILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQIAGRADRKGPYTNSRVYFFYE
                     EKTSAIRKACQEIKEMNEEGKQML"
     gene            23044..23648
                     /locus_tag="IVY85_001547"
                     /pseudo
     CDS             23044..23648
                     /locus_tag="IVY85_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
BASE COUNT         7815 a         4125 c         5092 g         6616 t
ORIGIN      
        1 attttgctaa tccagctctg tttattcagc ctatttttct ttttgaatat atcaagcgtt
       61 gtggcaacat agtgatgact aatgaaacaa cgacgataga caacaaacga tcaaggtagt
      121 ctgtacctgc ctgtaccaag atcacacttg ttgtctgact taaacctaac ccatgtaata
      181 gctgaacgat catactggat cctgaggagg tgatcccgcc aaataactga acggtaatga
      241 tcgaagaaac gatcgttcca ggtaccgaca aaatcaaagc gagtaataac agtttcaagc
      301 tgtttttatc tttgccgaga tatcttggca aaatcaatcc agcaagcaag cctgtgagaa
      361 tttggattgg cgaataatac agagaaaaaa tatctgtagt cactccactt aaaataccgc
      421 tggacaatcc tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca aaaatcgttc
      481 cgatagaatc gagatagatt ggcaaacgaa gccataaagc aatcgttcct ccaatataat
      541 tgatcccaat acacaaagcc atcactgtaa gtacgcgagt tgccattttg tttttattca
      601 ttttgttagc ccatctttaa cctttccaaa tctttcataa tcgtttcttc ttgtgcattt
      661 aaaagaacgg ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt cataatttta
      721 ctgttagcag gtttctgcca aaattcataa cgatcaacca atgtttgtcc acgacttatt
      781 ccttgagttt ccacagcagt gaagcttttg aaaccttcac agatggcctc ctcgataaaa
      841 taagcaacag ctaacggatc attgatgaca catcctaaaa ttctttcttg tttccagtgg
      901 aagtcaaaat aaaaacgagt gatcgctttt agatactctc cctcttcagg attcatttga
      961 cagcagtatt cgaggatagt cggtgtcaag acgatttctc ttgtcacatc taacccaacc
     1021 atttctatcg tttgtttgag attctcgaat acataagaag ctgcttcagg atcacaccag
     1081 taattgtatt cagcaactgg cgaacagtta ccatggcttt tgtaagttcc acccatcgag
     1141 acaaaacgtt ccatatgttt cccaatattt gggttgactt tcaaagcaga agcgatattg
     1201 gtcaaaggac ccaaagcaat gactgatgta tttgtctttt ctttgaaata atcagccaaa
     1261 aaatcaacgg catggagagg ttctgcctgt tttctcaaga tcatcgggaa atttgtttcc
     1321 cctaaaccat ccataccatg agtgtcttgt gcactgatga aaggttgctt caaaggtttt
     1381 tccattcctt gataaacagg aatgtctaaa cgatcacagc gttccaggca tttcagcaca
     1441 ttctgcgtac ctatatctgc agggacattc ccacaaacta cggtaatagc aactacttcg
     1501 atatcagggg atttgacagc taatgataaa gctaaagtgt cgtcgatccc tggatcgcaa
     1561 tcaataatta cttttcgcat tatttccaat ctcctttctt tagagatact tagtttttta
     1621 ttctgggaag tttccgaacc agtccaagac gagcgtcttg attataatgc acgaaattag
     1681 tatactggct tttattgcga atgtaaatgc tctcttacaa gtaaatctat tgaggagaac
     1741 tttaagtaaa tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa ataaagaatc
     1801 ctatggatcg gtacattgga taatagatag attatagttt ggcagaaagc ggcgggtgta
     1861 caatggttat tttttatttc attcttattc tgataattat agggatcagt taccattttc
     1921 tttcttattt ttatgcaaag aaattagtaa gagcgggagt acagagagga tatgactggt
     1981 atgaagacac tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta gaaaaaaaac
     2041 gacaaaaatt agaagaagag tgtgaaagct tttgggagca gggaacaccc aaaacaatca
     2101 aaagttatga cgggttgaaa ctagctggtc agatgttcat ccatccaaat aagcagaata
     2161 aatgggtcat ttgcgtacat gattaccgaa gcacagggaa aagagacatg tctcatatag
     2221 gtaaaagata tgcagagaaa ggattcaacg ttcttgtacc tgatttacgt gcgcacggag
     2281 aaagtgaagg agaaatcatc ggaatgggat ggctagatcg gctagattta attgcctgga
     2341 tccaactcat cttagatgaa cagccgaatg cgtccattat tttacacggc ggatcaatgg
     2401 gagcttctac gatcatgatg gcaagtggtg aaaaacttcc atcagcggtc aaaggattta
     2461 ttttagacag cggatatgtt tctgtttatg cagaattccg atatatgtta agtaaaatca
     2521 ctgtctttcc taaaaaaatg gtcatgagat atgcgaatca ttatgcccag aaatacgccg
     2581 gttactcctt aaagcaagca tcagctacga gacagcttgg cagcaatcat ctgcctttat
     2641 tagtcatcca tggtgaaaga gatcattttg tccctacaga agcagcgtac acgattcaaa
     2701 atgctacggc aggggataaa gcactgctgc ttgttccgga agcagaacac ttggaagcat
     2761 cgatgaaaga tccgaaaacc tattggacag tgattttttc atttattgaa caaagaataa
     2821 cgatgaatta attttctctt cttttttgta agtaattcga ggataaataa ttgtttatag
     2881 atcaggagac tgctaaaata aggtaggaat aaaaaaatat cagttatttg aggagtgcgg
     2941 catatggaaa tcaaagaaga aaaaatcgtc ttgctttatt caatgatgaa caacaagaga
     3001 tcggagaaat gacatggtca gatgcgggcc cagacatcat gatcgttgac catacatttg
     3061 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
     3121 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
     3181 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     3241 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     3301 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     3361 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     3421 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     3481 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     3541 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     3601 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     3661 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     3721 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     3781 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     3841 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     3901 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     3961 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     4021 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     4081 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     4141 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     4201 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     4261 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     4321 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     4381 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     4441 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     4501 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     4561 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     4621 agtgaaactt cctttaaaac caatcctgat cgaaggaaca tttcgagcaa atgaacgagg
     4681 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     4741 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     4801 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     4861 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     4921 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     4981 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     5041 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     5101 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     5161 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     5221 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     5281 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     5341 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     5401 tgtatatttg actgatcgag taattccgat gatcccgcaa cgtttatcga atggtatctg
     5461 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     5521 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     5581 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     5641 tcagctcgta cctatgttcc aagaaatggg tgaattgcac cgaatccttg aaacgatgcg
     5701 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     5761 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     5821 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     5881 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     5941 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     6001 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     6061 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     6121 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     6181 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     6241 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     6301 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     6361 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     6421 gggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     6481 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacagaaag tgcgagtaaa
     6541 agtagtcaaa gctgatccag atgaacgagc agtagatttt gaacttgttt ccgcagaaga
     6601 ggttacagcg ttggaacggc caaaaacacg aaatggaaaa tcaggaaaac gcgaaggaaa
     6661 acaagcagga aaacgcccag aaagaaatgg caagaagaac aaacgatata gcgatagtaa
     6721 gcaaacgttc ggtaagaaaa aagggaagaa gccattttac aaaggtgtta aaaagaagaa
     6781 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaattaat
     6841 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     6901 ggtcttgcaa ggaaccgaga tcaagtcgat acgaaacagc cggatcaatc taaaagatgg
     6961 atttattcgc gtccgcaacg gagaagcttt cttgcataat gttcatatca gtccttatga
     7021 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaaaaa
     7081 acaaatcatc cggcttgaaa atgaattgaa aaatactgga atcactgttg ttcctttaaa
     7141 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcgaaag ggaaaaaatc
     7201 ttatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     7261 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     7321 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcat aggttttttc
     7381 ttagatcagg agcaccgtgt ataaagaaaa attcgcttcg tttttgactg aaataaaaaa
     7441 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     7501 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     7561 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gttccacatt ggaccaattt
     7621 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     7681 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     7741 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     7801 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacatcggtt
     7861 tagtaacaaa aatcgtttta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     7921 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     7981 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     8041 ttccgatgaa actgattgaa gaatttacaa acttattaac acttgcttta cgtctttatg
     8101 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     8161 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     8221 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     8281 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     8341 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     8401 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     8461 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     8521 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     8581 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     8641 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     8701 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     8761 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     8821 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     8881 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     8941 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     9001 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     9061 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     9121 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     9181 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     9241 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     9301 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     9361 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     9421 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     9481 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     9541 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     9601 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     9661 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     9721 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     9781 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     9841 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     9901 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     9961 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
    10021 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
    10081 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
    10141 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
    10201 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
    10261 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
    10321 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
    10381 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
    10441 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
    10501 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
    10561 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
    10621 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
    10681 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
    10741 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
    10801 cggttgatgc cggactatct gtatctcgtg taggaggttc tgcgcaaatc aaagcaatga
    10861 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
    10921 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
    10981 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
    11041 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
    11101 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
    11161 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
    11221 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
    11281 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
    11341 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
    11401 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
    11461 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
    11521 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
    11581 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
    11641 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
    11701 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
    11761 tctgatcagc caagctttga tgaagtccgc aaaattgtaa atatggcaac aactatgttc
    11821 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
    11881 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
    11941 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
    12001 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
    12061 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
    12121 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
    12181 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    12241 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    12301 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    12361 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    12421 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    12481 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    12541 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    12601 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    12661 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    12721 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    12781 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    12841 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    12901 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    12961 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    13021 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    13081 attgcaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    13141 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    13201 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    13261 ggcttcttct tcaagtgctt tagcacctga aatcgtggga gaagaacatt ataaagtagc
    13321 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    13381 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    13441 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    13501 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    13561 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    13621 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    13681 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    13741 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    13801 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    13861 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    13921 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    13981 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    14041 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    14101 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    14161 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    14221 tggaacaccg tatatcagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca
    14281 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    14341 tgaacaccgt ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt
    14401 tgcatatttt tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat
    14461 acttgttttt ccttttatac attttatggt attatcattt tattagttat tataaaacaa
    14521 tcgttagaaa ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga
    14581 gtcatttggc gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt
    14641 ttttcagacc aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt
    14701 tagggtacac tgcaagtaat tttttcttag aggttatcgc tttatgccga aatcttttcg
    14761 ttagcctgta aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa
    14821 gattttgagt aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca
    14881 tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg
    14941 ttttgcctat tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg
    15001 ttccgattct ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg
    15061 tagattttga tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg
    15121 aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac
    15181 ttgccagaaa tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac
    15241 caatcgattt gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg
    15301 gctatatcga agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta
    15361 gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca
    15421 ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg
    15481 gcgctcaagt ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac
    15541 atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg
    15601 ctgcaatgac acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat
    15661 tgatttctaa attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg
    15721 tcatcggacc aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc
    15781 ctacagatat gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtgg
    15841 tgactgaaac agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg
    15901 ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg
    15961 cagaagtata tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag
    16021 caaatggaat cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc
    16081 atatcaaact acagcaatta ggtgcagacg tcgaacgtgt agacatggat caaacttctg
    16141 ctgaaaaaac agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata
    16201 tcttattaac tttgataaag attttagtgg tgatcttatt gcttatcctt ctttttgtgg
    16261 ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtattccagc
    16321 caagtttgtg gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc
    16381 tagtcgatat gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt
    16441 gacgttagtc gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa
    16501 gatattgatt tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag
    16561 ctgccgttat tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggtgc
    16621 gctaaaaggg acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg
    16681 cggtatcgat tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga
    16741 accagattat aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaaaagagg
    16801 aaatttattg tcaaagtaac aactccgcaa ctacttatat gggggacaaa aaatgatcga
    16861 agaactaaac aagaaagatt taccatggtc cttgttatta gatgcagatc ctgataaaga
    16921 aaaagttcaa gtatatgttt ccagagggag tggctggata tggaaagaaa aagaaaaaac
    16981 aataggtgta ctgatctatg tggcaaggga aaaagaatac gaaatcgtca atgtagcagt
    17041 aaccccaacg tatcaaggaa aggggattgg cggaaaatta cttgagacag cttttcagaa
    17101 gctttcacag ctagcttcaa accaaactcg gatcattata cgtactggca ctactagtag
    17161 cgctgcgtta cacctctatc aaaaaatggg ctatgtggag attggtcgag tcaaagacta
    17221 ttttatccat cattactctg agcccatttt tgaaaatgga gatcaattaa gggatcaagt
    17281 gattttagca attcacttac ctaaaaatag ctttaactaa taaactttca attcgagaaa
    17341 atgctttcaa atgctaaata gatacattac aatagcttta gcaaacaaaa aggagaatat
    17401 ttttatggga atattagttt ttgattttgg tggatcggct gtcaaatatg gttgttggga
    17461 tggcaaagag attaaaggta agggacaatt tgcaacgcct gaatcttggg aagaaatgaa
    17521 aacgcaatta tttcaagttt ataaaaaaat actcgtttct tttgaagtcg aaggaattgg
    17581 aatcagctct cctggagtag tgaataatga gaaacaggtg attgagggaa tcagtgcgat
    17641 tccttatatt catggattta atattttccg agatttggaa actctgtttc aactcccagt
    17701 aacgattgaa aatgacgcca actgtgccgg aatggctgaa ttttatgaag gtgctgcccg
    17761 tgattatcaa gatgttgctt ttgttgtggc aggaactggg attggcgggg cgttgttcac
    17821 gaaaggacaa ataaataaag gggcacatct ttacggtgga gaatttgggt tgatgttctt
    17881 agatggcgat aagacattca gtaaacttgg tacagcggta cagatggctt ggcgttattg
    17941 tgaacgcaaa ggtctcgata aaaatgctta taccggaaaa gatgtctttg aattagcaga
    18001 aactggagat gcaatagcaa aagaagaagt tgagagtttt tatacgtatc ttacaaaggg
    18061 attattcagt attcaatttt cgtttgatcc agaagtgatc gtattaggcg gcggggtttc
    18121 tgcaaaagaa gggctcattg atgagatcaa aagccgaatg aagaagctga cagaacaatt
    18181 cgatctgcat gatttcgaac cgcagatttt attatgtgag tacagaaacg atgctaatct
    18241 tataggtgca gcagcaaact atctcgctgt acgtcaagga gagaattaag agctttagag
    18301 aaggtgcagc ttgcataaaa gaagagaatg aaatgaatag gagattttta cctaaggaat
    18361 gttttatttg ctgcagtatg ataaaatatc tgagaggtga aaatgatgtc aaaaaaaaat
    18421 aaagatattg aagtgcgtat agaagaagtg aaaaaagata tcaaaggtaa gaattatgaa
    18481 gtcactcagt tatttatagg gaaaaaaatg attggtgaga tcttagccta tgggccaaaa
    18541 gaatatgaaa ttttctttgg tgaagaagat tttggaaaag aaaaaagtct tgaaaatgcg
    18601 atagaaacag tgattcgaca ctggaatctg cacgaataaa aaaatatcaa aaaaactttc
    18661 aataaagggt tgcaaaaatc tggaaaatta ggtattataa ctaagtcagg ttgatacaaa
    18721 cctttcggag gagtagcgaa gtggctaaac gcgacggact gtaaatccgt tccttagggt
    18781 tcagtggttc gaatccactc tcctccattt cattggggta tagccaagcg gtaaggcaag
    18841 ggactttgac tccctcatgc gttggttcga atccagctac cccagtatac cacaatcgta
    18901 agctctcgtt agttcgagag ctcttttttt ttgctttgct accttgttcc ttgaagattc
    18961 tgctgaatta agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga
    19021 aagaaaaagt agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca
    19081 atattgaagt gaatattccg aaaaaaggcg gaaaagcggt ccaggtaaaa tcaaaacgcc
    19141 caacgaatca tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata
    19201 agtcggcatt ttcatcgatt ttagggtctt ttgttatttt ctaggttgaa catatctaaa
    19261 aaattgtttc tgacaatagg agtagggaga acgatgtgtg tctaaatcct ctgttgatgg
    19321 atagaaacct ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa
    19381 cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc
    19441 aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa
    19501 gagaattagc agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact
    19561 tggcagatga aggaatctta gaaagaaaga tcggttcggg tacgtatgtt gcgcgagaaa
    19621 aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcacaaaatc
    19681 gagtaccgtc tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg
    19741 aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag
    19801 atgatgtccc tatttgtttt gaagttgcca gtgttccaca aaagctgatc gatggctaca
    19861 gcaaagcaga gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga
    19921 tcggtccagc gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt
    19981 tagaggtaaa acgaagagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg
    20041 gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg
    20101 aaaaataaaa aacaggtgat ccctgattcc ttattagaaa taaaagagaa tcagaatcac
    20161 ctgtttttat atatgatcag ttatcagttg ctcgtgataa gaaacagaac cctcttcaaa
    20221 ttggacttgt ccgttcagca gctcaatgat ggattcttta aagtggttag cacttttttc
    20281 atctacagca cagatgacgc taacttgatc agtatattgg atttctttta taaaaaaatg
    20341 ttggagttca agataattct gtaattttcc tagttgcgga tacgtgatca tcacagagat
    20401 ctcctgttgc aaggtacctt ctacgattcc aatgtcagac aatgcttgag agaccgcatg
    20461 agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac
    20521 tactgcagct acattgatca gttgattttt tttgaggact tccaacatcg gtacgcccgc
    20581 cgttccactt ggttctccat catcgctgct tcgctggatt tcattttttt ctccaatgat
    20641 gaaggcgctg cagttatggt tcgctttcca atgttctttt tttatttctt gaatgaaatt
    20701 ttttgcttgg tcctctgatt ctattcgtct aagagaacag ataaagcgtg attttttgat
    20761 ctcaatctca ctttgtccat tttcttttat tgtacgatag cgctcgatca tcttgtcacc
    20821 tctttttttc attttcttta ttcagtataa ataaagttct aggttgtttc aacactgtaa
    20881 cggttgcgca attgctgtag tatgcgataa taaagacaaa ggcaggtgga tctggggatg
    20941 caggaacgaa tgaaaaagta cgatgcaatt acgcattatt tgaagaacaa tggcggctct
    21001 caagtaacgt tgacctttac ccaatttgac gaactgctgt ttcccagtaa cggacttccc
    21061 aaaacagcaa gggaaagtac agattggtgg gcaaatgatt ataaacaccc tgaaaaaggt
    21121 gcctatggct ggataaatgc aggctatgaa gtagttgtca tcaatttgga caaagaatat
    21181 gtcgtgttca ataaattagt gaaatctagt tggctatttg attaaataaa ccgcgtttag
    21241 aaaacgttga caaatttctt tctttttata taataaagta acagacgcga agaaaacgtt
    21301 tgcgtagttt gttttaaaaa ggagagaaaa atatggctaa aaaaacaatc atgcttgtat
    21361 gttcggctgg tatgagcaca agtttactag taacaaaaat gcaaaaagct gctgaagcaa
    21421 aaggaatcga ctctgatatt ttcgctgtgt ccgcttctga cgcagataac aatcttgcaa
    21481 ataaagacgt ggatgtgttg ttattaggcc cacaagttcg ctttatgaaa gctgattttg
    21541 agaaacgtct ggaaccaaaa ggcattcctt tagatgtcat caatatggct gattatggta
    21601 tgatgaatgg cgaaaaagtg ttagatcaag caatcgcgct cataggtgaa taaatagaac
    21661 agtataacta tcgaacaagc gatccaaaaa atagatcgct tgttttttcg tatatttttt
    21721 tagggcgaat aatatgaatc atttttgggg tagaaggctt ctgacgagag atattaaagc
    21781 aatagactgt gaggaggtaa atttaccaaa ttatgagaag atcgcagcag tggaacgttt
    21841 gggcaactgg attctttgtc atcgctgtgg aatgaggacg tccgtcattg ttggacagct
    21901 tgcagattac ggttattttt gtacacattg tctatattta gggtgatgcg acactcaaca
    21961 agatttatat ttgtttgatc agcctgaagc aaaacctcga gaagtcgtct ttttttggac
    22021 aggacagctt actgaaaaac aaacggaaat cgcaaagaga atactttgcc attcagaaaa
    22081 aagacatcat cttatttggg ctgtaacagg tgcagggaaa acagagatgc tttatcctat
    22141 tttagtggaa actttaaaag ctggaggaag agtagcgatt tgtacaccgc gagtcgatgt
    22201 ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca gaagaaaaaa tcacgttgct
    22261 ccatgggaat acagcaacgg cttatacctt tacttctttt gttatctgta cgattcatca
    22321 gctataccgt ttttatcggt cctttgattt aatagtgatc gatgagatcg atgcgtttcc
    22381 ttataacggg gatgctgggc tagctcacgc tgtgcaaaca gcaatcagat caaacggccg
    22441 attcatctac ttaagtgcca cgcctgatga gaagttacta aaggaaatca agcagacatt
    22501 tgagctccac aaacttgcct tgcgctttca tcggcgcttg cttcctgaac ccgtgcttgt
    22561 tttcatgaat aattggcgac aaaaatgtgg aaacaaaaag aaaatcaact ctttggtgcg
    22621 gatcattcag caactaatca aagaaaaccg tatcctttta ttttgtccaa gtattacgat
    22681 gatgcatcgt ttaaaaatca cactgcaaga agcttttcca gaatatgaga taacagatgt
    22741 ttcttcacaa gacgatcaac gggcaaagaa agttcagaaa atgcgagaag caatatacga
    22801 gatactgtta accacaatga tcctagaaag aggagtcaca tttgaaagaa tctctgtcat
    22861 cgttttagga gcagatcatc cagtttttac taaatcctct ttggtgcaaa tcgcaggaag
    22921 agctgatcgt aaaggtcctt atacgaacag tcgagtctat tttttctatg aagaaaaaac
    22981 ttcagctatt cgaaaagcct gtcaggaaat caaagaaatg aacgaagaag ggaagcagat
    23041 gctgtgagat gtagttgctg cggtaaagac atcattcaaa atttaacatt cagcgagatc
    23101 atcggagtgt cgaaatctag acttcctcag acatgttcag tttgccgcgc acaattccat
    23161 ttattagaag caaaaggttg tgcaggttgt caaaaaccaa tccatttgaa atacgaggag
    23221 acgaaaaaga aagcaaatac tagtttcgtt tactgcaacg actgtcaaga atggaagaag
    23281 aaatatcctg catatgattt tcaacaccgc gcctttttta cgtacgatga aaagatgcag
    23341 gcttggatca atcggtacaa gtttatggga gatatccgat tagcagggac ttttgcatat
    23401 aaatggactc agataaaaaa aagattaccc ggcatatatt tattgtccaa tacctttatc
    23461 tgaaaaaagg tggctagaaa gaggattcaa tcaagtaagc gagatgttga aagcagcaaa
    23521 aattcctttt cagatgctgc tcaaaagaga gcaacatctg cttgctcaag caaaaaacac
    23581 gcgaaaggaa agattggaga tgcctcagcc ttttgagctt gccgtacctc tttctaagat
    23641 acaacata
//
LOCUS       DACCYH010000041        23450 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.041, whole genome shotgun sequence.
ACCESSION   DACCYH010000041 DACCYH010000000
VERSION     DACCYH010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.041"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            39..356
                     /locus_tag="IVY85_001548"
     CDS             39..356
                     /locus_tag="IVY85_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8044570.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            349..957
                     /locus_tag="IVY85_001549"
     CDS             349..957
                     /locus_tag="IVY85_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP8044571.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            973..1803
                     /locus_tag="IVY85_001550"
     CDS             973..1803
                     /locus_tag="IVY85_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP8044572.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            1941..2429
                     /locus_tag="IVY85_001551"
     CDS             1941..2429
                     /locus_tag="IVY85_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP8044573.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            2480..3352
                     /locus_tag="IVY85_001552"
     CDS             2480..3352
                     /locus_tag="IVY85_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP8044574.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            3528..3890
                     /locus_tag="IVY85_001553"
     CDS             3528..3890
                     /locus_tag="IVY85_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8044575.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            3899..4651
                     /locus_tag="IVY85_001554"
     CDS             3899..4651
                     /locus_tag="IVY85_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8044576.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            4665..6467
                     /locus_tag="IVY85_001555"
     CDS             4665..6467
                     /locus_tag="IVY85_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8044577.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            6744..7784
                     /locus_tag="IVY85_001556"
     CDS             6744..7784
                     /locus_tag="IVY85_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8044578.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(7836..9980)
                     /locus_tag="IVY85_001557"
     CDS             complement(7836..9980)
                     /locus_tag="IVY85_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8044579.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLRLDKSFWLDLPLKK"
     gene            complement(10337..11710)
                     /locus_tag="IVY85_001558"
     CDS             complement(10337..11710)
                     /locus_tag="IVY85_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8044580.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(11710..12105)
                     /gene="rbsD"
                     /locus_tag="IVY85_001559"
     CDS             complement(11710..12105)
                     /gene="rbsD"
                     /locus_tag="IVY85_001559"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP8044581.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(12133..13050)
                     /gene="rbsK"
                     /locus_tag="IVY85_001560"
     CDS             complement(12133..13050)
                     /gene="rbsK"
                     /locus_tag="IVY85_001560"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP8044582.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(13158..14171)
                     /locus_tag="IVY85_001561"
     CDS             complement(13158..14171)
                     /locus_tag="IVY85_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8044583.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(14252..14851)
                     /locus_tag="IVY85_001562"
     CDS             complement(14252..14851)
                     /locus_tag="IVY85_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8044584.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            15037..15738
                     /locus_tag="IVY85_001563"
     CDS             15037..15738
                     /locus_tag="IVY85_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8044585.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            15748..18459
                     /locus_tag="IVY85_001564"
     CDS             15748..18459
                     /locus_tag="IVY85_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8044586.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            18910..20538
                     /gene="treC"
                     /locus_tag="IVY85_001565"
     CDS             18910..20538
                     /gene="treC"
                     /locus_tag="IVY85_001565"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP8044587.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(20598..21779)
                     /locus_tag="IVY85_001566"
     CDS             complement(20598..21779)
                     /locus_tag="IVY85_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8044588.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            22031..>23450
                     /locus_tag="IVY85_001567"
     CDS             22031..>23450
                     /locus_tag="IVY85_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8044589.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAG"
BASE COUNT         7629 a         4195 c         4785 g         6841 t
ORIGIN      
        1 gaaataaaat attacacttg agaatagaag gtgacgtaat gcacatccag attccaactg
       61 ttcttcttga tggtactgtg ctagcagcat taagcaagga agatatgtat gggtatgcct
      121 taacaaaaat cgtacaagaa agtctctctg tcagtgaatc tacactatat ccggttttga
      181 gaagattgaa aaaagaagga tatcttgaga cctatgatga accttttgaa ggcagaaacc
      241 gtcgatatta ctcgttgaca ccaatgggaa gtcgtcattt agaagaaatt attgatgaat
      301 ggattgtttt tcgagattct ttagaccggt tattggagga gagcacaaat gaatgaatat
      361 ttgaatgaag ttgtggatca tcttcaagaa attcctgaag acgatcgttt tgatttgatc
      421 cagtattatg aggaatattt tttagattct ggaaaaacat tagatcagat cattgatgaa
      481 tatggtacac caaagcagtt tgccttaaaa ttaaaaatca gttacttttc agaagaagaa
      541 caagcagaaa acgagttagt gaaaaatcca aaaagacaaa ttcgcctgat ttggttaatc
      601 attatcggtt tatttgcttc tcctgtcctc attccactag cactggggtt tatattggtt
      661 attgggggac tattggttgc tttactagcg ctcattttta gtatctatgt tttatgtatc
      721 agtatcttag gtgttggcct attcatgatc atctcaggaa tcagtgtgtt tggacaatcg
      781 attctgagtg gattattctt tatcggtgtc ggtctgttcg ccacaggaag cgcagtattt
      841 atttcgccac tgttgctgaa aggaacaaaa gggctttttt atgtcatgat gaactttgtt
      901 aaatggatgg gacgacgttt tattacaaaa agagatctgc attcagcggg cgtttgaagg
      961 aggacacata aattgagatt gaaacatttt ttgattaccg gtattggtct gatgcttact
     1021 ggtggcctcc tagcaggtgc aacttataca ttaggcgctc aaaagagtct gacttgggat
     1081 aatggtcctc ggttgatcga tatggttact gagacaaaaa aaatcaagga tgcaaacaaa
     1141 atcgtcatcc atgcagaaaa tcaaacagtt tcggtcatac gcggattgga ttttgagata
     1201 aaaacgactt atgataagac agaaaagcca gaaataaagg agaaaaatgg cgtgttgact
     1261 gtgaatgcag tcaaaaaatc aacaggtgca atcattagtt tagaagacac aagtgcacaa
     1321 atcactattg ttgtccctgc agatcatgaa ttatctgaat tgacaatcga agggaataat
     1381 agtgcaattg atatcaacga agtatcaatc aaaaaagtga atactaagac acatgatggc
     1441 tgggtatcgt tcaatgaatt tacaggagga acaataactg cgaaagatga attaggctca
     1501 cttcgattag atgaggccaa tgtggataac ttgatcgtca caggtgaaaa cagcaacttc
     1561 catctagaag acagtttgat cagcaaagaa ggaaaagtca tcctaaacgg tggcaattta
     1621 tctttgcatg agatggaaaa cagtggctat actatccaaa cgtctggtga atcttatatt
     1681 gaaaaagact accagccaat aacaaatact ttgttagaac aaggacagaa aaagctaaag
     1741 atcactgcta aagacacatc gatcaatatt acgacggatc aagaggacaa tgaaacagat
     1801 tgatcctgta ctcaactgga tattttaaga aaaaacagca aagatccttc caatctttgc
     1861 tgtttttcat cgcttaacgc acataactat agtaaaataa gaaagaaaat atctaaaaag
     1921 aaaacagggt gaaagagtat atgaaggtag aagaaggtca aagagaaaag ttaaaaacag
     1981 aaatggaaag attacatact tatatcactc aactaagcca aaccttctat gatccagaca
     2041 aagaaaaagt gatggttaat tacccgaaca attcagaagg tcgccaatta gagcaagtgt
     2101 accatgaagt tttcaaacat ttattgacgg tgaagaaaga gctggattat tattctttac
     2161 cgattatcga tacaggaata ttgaagtatg atgggaagaa ggaacgattt atctttaaat
     2221 ctgttcggga agatttgcct ctttcagcag ggatggatct agaggtcttg gtagaagact
     2281 attttacaga agaaaagcac tgggttcgaa caaaattgga ttatttaccg caggcagccg
     2341 gagggacaca ggcaagcggt tggtacatca ctgaagacaa agagttagaa ctagaaggtg
     2401 taatggcgcg tattcgaaaa aaaaattaaa aaagtaacat tagatattta ttgaaggata
     2461 aaggagcgca ggaagtagca tgagagaaaa aagagcattt tatatcaatg gttatgcagg
     2521 gattgtcgga ttggtcgtat tagctttagt tggattgttt ttcttctatt taggtatgtg
     2581 gcaagcgaaa gtttgggcac tttttttaag tatttttctt tggttgatca cgttattgtt
     2641 gttaagttcg gcaactgtag tcagtcctaa tcaagcaaaa gtaattttgt tttttggaca
     2701 atatttaggt acgattcgag aaaatggctt tttccttacg atacctttgg cacaaaaaat
     2761 gactgtttcg ttgaaagttc ggaatttcaa tagttctgtt ttaaaagtca atgatttgga
     2821 tggaaacccc atcgaaatct cagcagtagt agtctttaaa gtcgttgata cagcaaaggc
     2881 attattcgat gtagcgtatt atcaagattt tgtagaaatc caaagtgaga cagccattcg
     2941 gcatatcgct agccaatacc cgtatgatac attcaatgag aatgatttga cgcttcgagg
     3001 aaacacgacg gcagtgtctg acgagttgca aaaagagctg caagagcgtt tagcagtagc
     3061 aggtgtcgaa gtaatcgaaa cacgcttgaa ccatttagcc tatgcaacag aaatcgctag
     3121 tgccatgctc caacgtcaac aagcaaaagc cattttgtct gctcgacaaa ccattgttga
     3181 aggagctgtc acaatcactc aaatggcctt agagcaaatc gaagatggac aagaaatcaa
     3241 tttcaccgat gatcggaaag tccaattgat caataattta cttgtctcaa tcattacaga
     3301 caaaggaacg caacctgtta tcaatacagg cgacatgaaa gaccgtaagt gacatacttt
     3361 ttgtaagata ggaagaagga gtttaggacg gattattgtc ttgaactctt tttttagttg
     3421 ttttaaagaa aatgacaaaa ttgaaagaag aatgatagtt gattcgaagg cagaattgtt
     3481 cgcttttttc tataatcagt atataaagta agagagaagg ggaaaaagtg aaaaaacttc
     3541 tgatcggtct acttgttttc atcgttgtgg tacttgcagg ggtgaaaatc gcagattacg
     3601 tagttatggg tggagaagaa tactatgtac aaattacgac agatggtaca aaaacagttt
     3661 caaaatcaga ctctggacaa gaaacagtca actatagata tatcctgcct ggctatacga
     3721 aagagggtaa ggaaaaagaa ctggacttca agggacaatt gactcgaccg cttcgtaaag
     3781 gagcatattt aaaaatcacc tggaacaaaa ataaaggtgt cacttcgtat gaagaagtcg
     3841 aaaaaaaaga cgtgccgaaa acagcactgg aaaaactaat taaagggtag ggaaagaaat
     3901 gaacaaaata gtcgaagtac agaatgtaac aaaagtatac ggaaaaaaca atgagaaaaa
     3961 gacgcaagca ttaagcggca tcagcttcga tgtagaaaaa ggcgaattta ttgggatcat
     4021 gggcgcttct ggttctggta agtcgacact tttgaatatt ttatccacat tagataaacc
     4081 aacagatggg catatccgaa tcaatcaaaa agatgtgacc acattaaaag gaaatcaact
     4141 agcagatttt cgtgcgaatg agattggctt tattttccaa gatttcaatt tattagaaag
     4201 cttgacagca caagaaaata tcgctgttcc tctttcttta caaggcgtca gaccaaaaga
     4261 aatcaagcaa cgtgtccaaa aaattgcaga acgtttatcc atctctcata ttttggaaag
     4321 ctatccttct gagatttccg gtggacaaaa acaacgggtc gccgccgcac gtgcattgat
     4381 cacacaacca acgattctat taggggatga gcctacaggt gcgctggatt ccaaaagtgc
     4441 ccgggatctt ttagatacga tggacgaatt gaatacaaaa gatcatgtat cgattcttct
     4501 tgtgacgcat gatccgtttt ctgcaagtta ttgccaacgt attttattca tcaaggatgg
     4561 cggtatccat caagaagtga aacgtggaga tcaaagccgt gattccttct atcgagaaat
     4621 actatctatt cttggaaact tggaacagta gggggagatt gactatgctt tggaaattat
     4681 cacttactgg gatcaagagc cgtttaagag actacttggt gttattctca ggattagtta
     4741 tggcatctgc tattttttac atgtttgaat caatggcaag caacgaagct tttttgaaaa
     4801 gtaatacgat cgttgcggct acagttatca ttttccgatt cggttctgtt ttactaggga
     4861 tcatcacatt cgtttatatt ttatatgcaa attccttttt gatgacgatg cggcaaaaag
     4921 actatgcaat gtttatgatg ctgggagcaa aaggcagaaa aatcgcacag atgatttttt
     4981 ctgaaacatt cttagtaggg atcgcagcta ctttagtggg ttcagctgtc ggtgtaggat
     5041 tgacaagtat cgtccaccga ttattagtgg atcagttaga tctcaatatt tcgcatttta
     5101 caccttttag tattcacggg ttgttgatca ctgttttgtt ttttgctgtt ttgtttttat
     5161 tagctgctgt tgtcaatgct ttttcgattg tcaaaaaacc gattttagca ttgattcgtg
     5221 cggatcagac accaacaaga atcaagcagc ataaattctt gtttttcctt gaagtagtac
     5281 tgggaattgc ttttctaggc tgcggttact atatgatgaa acacgtgggg acctatcaag
     5341 tctttggtat aggggtagcc ttagttacga tcgtattagg aacttatttc attttccatt
     5401 ctgtgatcat tttcttttta tcattactaa agaaatcaga acagatttca ttgaaaaaat
     5461 tgaacaattt cacattgtca cagttaaact ttcgtattcg tgaatataca caaatgctct
     5521 caatggtcgc tatgctgttt gccttggcac tgggcgcttt gacggtagga ttaggtttta
     5581 aaaatgaaat tgcagacttg acagaaaaca attctggata tgatctagtg atgaataatc
     5641 ctcgggcaga agatcagcaa aaaatcaaag aactttcacc aacactttct gcttcttata
     5701 ctcaaaaaga agatgcggga acgatttact ataatgcaag tgaatttgcg gctcaaccat
     5761 tgatcatgat aaaacatgaa gatggaacac cgccgaagat cacgaaagaa aaagttcctg
     5821 ctaaaaaaat gaatgaactg actgtacaag aggaacttcg aagctatgaa ttgccggaac
     5881 agcaagaaaa agaaatcaag gtagtcagtc aatcagaatt tgatcaactg aacttgccac
     5941 aatccacttt acagctagtt caggtaaaag acttccaagc aaacctgaaa ccaatcgaaa
     6001 cacttgttgc tcaaaataaa atgaacaatc catctttgca aaaagtagag tacagcaacc
     6061 agaaatatga aatgtatgaa gtgtataatg gtctattctc tggttttgaa tttatgggat
     6121 tcttcctagg tttagctttc ttgaccatgc ttgctagctg tttgatgttc aagatcttgt
     6181 caggaagcaa gagtgacatt gctcgttatg aaatgcttga aaagatagga acacgacgcg
     6241 ggttgcttaa acaatcgatc cgtagagaag taggcgtgtt attcttagca ccaggaatct
     6301 taggtgtagt ccatgtctta ttcggattac aaatgtttgg tctattcatg caaaatccat
     6361 acaaagatat ttgggttccg tttacgatat tcttcgtatt gtatttcatc tactatgtac
     6421 tgacgacatg gttatatact ggaatcgtat tgaaaaagag attgtgaaaa agctgtcctg
     6481 catcaagtaa taagaacagc caagcaaaaa tagctcctca tatttttcgt ttggttgggc
     6541 ttattaccgc agatgcaagc ttttgaacac cgtttgttcg gaaaaagagg ttgtgacaat
     6601 atttttgtca caaccttttt ttcacggtct tttattagaa tatagtaaat tttttctcgt
     6661 tactcacgga ataagctgtt aagaaaacgg tttccatgta tactggaatc agtgcaaatg
     6721 aaagaaaaga gggtgcaagg aacatggaaa tcaatgaaca aaattttggc gaaaactatc
     6781 acttgattac gatcacgaat caaaatggga catctttgtc tgtcagtgat cttggggcac
     6841 gggttgttca ttttcaaaca ataatcgata atgaaaaacg agagttgatc ttaggttttg
     6901 atgaagcaaa agaatatcta gaaaaagacc cttatatcgg tgcttcaatc ggacgtatgg
     6961 ctggacggat tacgaatggc acattcaaat tagatgatga aacgttcgaa gtaaaaacag
     7021 acccggacac tggtcattgt ttacatggtg gtgcacctag ctttgaagcc aagcagtggc
     7081 attatacagt catcaatggt gaaaacgaag catcggtcat tttctatcag actagcccag
     7141 atatggaaaa cggatttcct ggaactttag atgtagaagt acggtatacg ctgacgaacg
     7201 atgatacatg gcgagtgacg gtccaaggga tcagtgaccg caaaacagta ttcaatccta
     7261 caaatcatgt ctatttcaat ttaaccggtg atccagcaca gtcaatcgac gaacatatac
     7321 tttggttgaa tagccgatta tttgctacgc tcaataaaga tacaacgcct acaggagaag
     7381 tagtttcagt aagtggcaca gcatttgact tccaacgtcc taaaaaatta ggtgaggtct
     7441 ttgccagtga ttttcgccaa tcaatgatag tgggcggcat cgatcatcca tttttgttga
     7501 aaaaacaatc tggtttacta aatgctgcgg aattgacaag cccagatgaa aaagtatcga
     7561 tccaagtaaa aacagatgct tcaggaatcg tgatttttac agcaaatttt ggagaagatg
     7621 gaccggaaat gcgcgggaat aaattagtaa atcatggcgg tatcacattt gagactcaag
     7681 aactgccagg agcggagcga cttgaaagtt ttggagatgt cactctggaa cctaaccaag
     7741 tacgcgagtt tgtaacggaa tacaaaataa atataaagaa atagaataaa ttaaaaaggg
     7801 agtgtgacag tgtcacatcc cctttttaat ttattctatt tctttaaagg cagatcaagc
     7861 cagaatgatt tgtctaacct taacaaggtt agtacaggaa cagttgcagc tttaactgta
     7921 cgagtagttg tttctggtat ctgtcttgcc gtaaaattgt tccacaaatc ccagattgaa
     7981 gcgacacgga agatggaaga agcgttgttg acgcgtttta tcgttgaaaa ttctaatcct
     8041 gtgattggca cgccatctgt tgtccatttg attggcacat cgaatcgctt agacatattg
     8101 aggatttcag cgtttgcttc ttcagttgtt gctgcttctt ttttcggttc atcatatttt
     8161 gtcaggtcgt acgcttcgat gatcgcgttc aatttttgtg aatattggcg atctgtagca
     8221 taacgtcctt ccaaatattt ggttgcttct ttgtagtctt ttgtttccga cttccaagtt
     8281 ggtttgtaga aatcaggatt gccagtcagt ccatttttga tcaagtcggc atagtcttcc
     8341 aaactttcct tgtaagaagg atatttacgg aattgtgcgc ggatttggta gctttgtccg
     8401 gaactatctt gttcaagtgt attgaaggtc acgctgcttc ctttatatga tcctttgata
     8461 ccaaaaaggt tatgatttgg ttcgcttgat aataagctgt ttccagatcc actttcaagg
     8521 atagcttgag ccatcatgac tgatgcgtag attccttctt tttgcccgat ttcctgtgca
     8581 tcatctgcaa tctttttcaa gaactgatca gttgtttggt tttgactgaa atggaacgag
     8641 ccatctaatc catctaaaat agatgagccg cctgtcagcg aacctgaagc agcaggtaaa
     8701 cctgctaagt ttactcggac agcaaaatca ggcgtgaaat ctgaaataga agttactcga
     8761 actttatctc ctggttgagg cgcatggata aattgtccgt tcccaatata gatacctaca
     8821 tggtgagtaa caccggaaga aggtccccag aagacgaggt cgccaggttg ggcctgactt
     8881 actggaatct gcgtacctgc atattgctgt tcgcctgtcc agccaccgat ataatgaccg
     8941 gttgctttca tatatacgta ataagtcaaa ccagaacagt caaaagtatt tggacctttt
     9001 gctccccata catatggttt tccaagatgc ttgaatgctt ctcgaacgat tgcttgttgg
     9061 acattcgttg tatttgtagg caatacagca tcaattggtg attttacaac aaaatcagaa
     9121 gaagcagaag atggattctc tataccgctt tgatttgctg tatttggacg agatgaagca
     9181 ttgttcgttc cgccattcga tgaaccattg tttggatggt ttggttctct ctgagtattt
     9241 ccattgttat tcgatccaga tgggtttgtt tgattcgaag aattcccgct gttcgaatta
     9301 ttgttattat tgtttgaatt atttgaacca ttattattgt tgttattacc cacatctggt
     9361 ttgtttggcg tttccggatc tggtgtacta gggacttctt cactaggatc agttggtttc
     9421 tctgttgaag tatccggtcc agctttaaat tctgcagtaa tagataaatt gttcgtacct
     9481 actgtaaagg tctttgcatc ataatttact tctttggcac cattaacagc gactgaagca
     9541 atttgttcac cttcagctgg cgtaatagtg taagttacct tcgtttccgc taataaacct
     9601 tctaccgttc cactttccgt aatatccaaa ggttgctgtt caggggaatc cgctgtcgaa
     9661 gcagtcagtt ggatcttacc aatcaaagaa ggagcaattg taagggaata tgtctcggct
     9721 ggcttttcat tttctgatgg atcagccgga tcaatttcag ttggctcggt cgatcctgtt
     9781 gttgagttag ttgttccagt tgggtcttct gtaaaagaat ccgttgtttc actagtagct
     9841 gagctggatg tagtcgtatc tgttgtgact gtttgatcag ctatttgatt atctacggca
     9901 aatacggtat tggtcgacaa aacagacgga gcaatcagcc aagtagcagc tagcaaacgt
     9961 atcgtttttt tatttttcat aataaactag tccttttcct ttcattgatt ttctgtctta
    10021 ttcagcattc attagacatt ttttggaaca gataatcgtg tcgaaaagct ttttcttttc
    10081 ataaatctgt ctgtgtattc taaataaaac aatttgtaaa taagcaacga gcattcattc
    10141 gtttttcttt gacgttcatt ttattgcata gaccaccttt gtttggggat aaacaagtgg
    10201 ggataaaaat ggttatgcat atgtaaatta taccaaagtt tggaaagtat cacaaaaatt
    10261 tataaaaata atgatcctaa aaagaaaaag actgaaatat gtgataaaca tctcatactt
    10321 cagtcttttt cattaactat tcataagaca ttaggtcttc atcaaacccg ctaacgagag
    10381 tagtgatcat tgctacgact agactaacag ccagtcctgc taagtaccag aaaaaagtac
    10441 cggaaatgaa tgcaggcaat gtggtcagac taccaaatac aaaagcgatc gctttcactt
    10501 gggcaaatcc cattacagct ccaccagcag ctgcaccaat gcaagcagca acaaatggtt
    10561 ttttcaatgg gatcgcaatt ccgtaaacag ttggttcggt cactcccgca agtaaaatcg
    10621 aagcaaaagc agaaccggag atcgaacggg tttttgaacg tttacttcgc agaaatacac
    10681 ctaaagctgc accagccatt ccaaaattgg ctgctcctgt caacggaacg attcggtcga
    10741 acccataagc agagatatta ctgatcatga cagggtttac agcccattgg ataccaaata
    10801 ttaccaaaat attccaacca ccagcgatga ttgcgccact gatcggtccg ttaatatcca
    10861 tcatccaagt aattgcggca gctaaacctt cgccaaggta aacacccgca ggcccgaata
    10921 ccattaacgt caaaggaacc gtgataacta atgaaagtaa aggtacaaat actaattgca
    10981 tattttcgcg aatatatttt ttgaggaatt tttcaacaaa agaataaaag aaaataccta
    11041 aaatcgccgg catgacagta gacgtatact gcatcagtac tacaggaatc cccagaaaat
    11101 cagtagctgc tttgccactg tctgaaagta aagaggtcag attcggttca atcagtgctg
    11161 cagcaatgac agccgcaata tatccattca ctttaaacgt tttggcagca gaaaaagcaa
    11221 gaaagattgg cagaaaataa aagacggcat tcgacgcagc agacaaaata tgataagttc
    11281 cgcttgtttc actaagccag ccaatcgtag tcgctaaaac aaccagacct tttaatatac
    11341 cagaaccggc aaacagcgga atgatcggcg taaagatttc cgatatcaaa ccaaatagtt
    11401 tttcgatcgg atttagcttt tctttcggag ctgaatgatc gatttgacgc gtcaattcct
    11461 tgtaagcagg agccaatccc tgatccatga ttatttctgc atatctatcg agtaaaatga
    11521 aatacttaat ggaagggatt tgctgtttta atttttctgc attggctact ggctgttttt
    11581 tcaatttgat acgaagccga gtcgcacatg gaaggacaga atcaatattg tcttttcctc
    11641 ccaactgttc aatcagcata tgaatctgct cggacagtac catctgttca ggattcattg
    11701 tcgtttccat tagaagacca ctccactcac taatataata ttggcgtaag gtgtcatttc
    11761 gcctgtacga ataaatgcat gacagttatt caactctttt ttcatttctt catgtgggat
    11821 aaagctaatc ggtgtatctg gtaacaataa tttgattttt tccaatattt ccgggttcat
    11881 tgtctttatt tcttccgcca attcaatcga ttgtacctca agttcttcta aaacgttttc
    11941 taaaacagat aaaaaacttg gaagaccgtt tgagacagcc aaatctattt tttcagtacc
    12001 aaaggggaca ggcatgcctg catccccgat acttaattta tcaaagtgcc ccatttgtgc
    12061 gattactcgt gaaatatctg aattgattac ttttcctttt ttcatgtttg tttcttcctc
    12121 tcattgttaa ttttagactg ctgattgttc taaagcttcg cggtaaggga tagaaggttg
    12181 cgcaccataa gactgcacag caacagaaga ggctagattt ccataaagga tcgcttcttt
    12241 caagttacta aggtctggct gaagtgtgct gcttaatgcg ccaataaatg tatcacctgc
    12301 ggctgttgtg tcaacggcat ccactttaaa agcagggacg ataccgtgtt ctttttctgt
    12361 atgatagaaa gcgccggcac tgcctagtgt aatgattact gttccaatac ctaattcatg
    12421 gagtttttcg gctgctgcga ccaaactgtt atgatctgtc actctgattc ctgtaatgat
    12481 ttctgtttct gtttcatttg gtataatgat atctgtcttt tcaagtagtt cagtaggaat
    12541 cgtttcacga gctggtgcag gattcaggat cgttgttttt cctgcgtctt ttgcaataga
    12601 aaaagctagg atcgtcgcat ctaagctact ttcaaactga gcaataataa agtcactgtt
    12661 tgtaatcagt tgcttgtttt ttaggacttc ttgttcatgg aaagcattat ttgcccctgc
    12721 atggatcaag atactatttt ctcctgctgc gtctaccata atcattgctt gtccggttgt
    12781 ggcgccttgg atttcagcta tcccagatgt atcgatgttt tcttttgtca gtagatctaa
    12841 taactgttgt ccttcgctgt cagccccaac gcctccaata aaagatgtcc gtgcgcctga
    12901 acgtttggct gccactgctt ggttggcacc tttacctccg ccagcgtgaa aaatctcatg
    12961 cgcatgcatc gtttcgcctg gcttgggcat gttggttaaa cgaagggtag tgtccatatt
    13021 aatacttcca attactgtaa ccgtgttcat tttacaaacc tccaactatc tcatttattt
    13081 tgatgggaaa attatttttt aaaacgtttt agtaaaacgt tttaatagtt cgataaaaat
    13141 atagcacacc ttgaaagcta tttcaagggt gcagttgaaa atcttttttc taatcgtaca
    13201 ggtaatcttt tttcttccca ttctttttct gggaattgga tccgatctaa caataacttg
    13261 gcagatgttt gtcccaattc gaagattggt tgtgcgatcg ttgtcagttt cggcttgata
    13321 tactcgcaca tatctatatt atcatagcca ataatgctgt agtcttcagg gatgcttttt
    13381 ccagcttcct ctaatccgcg atataatcca aaagctaatt catcatttaa agcaaacaca
    13441 ccagttgcag cagaatcaag aagttctgct gtgatctggt agccaccttg ttttgagaac
    13501 tgtgtaggta agaggatcag ttggtcgtga gggatttggt aaacatcaag agcagattta
    13561 aacccctcaa tccgagcgtg gacattttcc ggtggatttt caggatagac aagggcgatc
    13621 gtttgatgtc ctagactaag caagtgcatg cccgctaggt aaccaccgcg aaaatcgtct
    13681 gttcgtacgg catcactaaa tccttcagat tttttctgat ccaaaacaat gaagggccgg
    13741 ccttgttttt tcaaattttc gttgattgca tctgtagaga cagcagaagt cgcgataatg
    13801 aatccatcca cacctcgacg tgtgagctcg gctagatatt cgatttcttt ttgatgatca
    13861 gaatcagcat tacataatat cgtgacgaaa ttctgcttat acaagatatc ctcaattcct
    13921 cgcatcagtg tactgaagaa tggattcgtg atatcaggaa cgagtacacc aattgttttc
    13981 gtttccttgg taatcatttg tctagcaaaa tagtcaggct gatagcctaa ttcttctttg
    14041 gcagctaaaa ctttctgaac agttttcgga ctgaatttct ccgtatttcc atttaatatc
    14101 aaggagacag ttgtgattga aacgccggaa agtttagcga cttctttgat tgtgatcttt
    14161 tttttcacca tgttcatact cctatctaaa tcctgtggtt attttcccat gaaatcaaaa
    14221 agaagtaaat gaaaatcacg tgtgccagta cttatttgct ttgttggata gcttcgatta
    14281 tttgtttcgt tgagcgtact cgtgcagttt tcggaaatat cttttttatg aacaattgat
    14341 gggattcttt gtcacgatca ctgcaagcat cttcagcaag cacgatatgg tacccgtgtt
    14401 gaaaggcatc taatgcagta gcatatacac cgtttgttgt cgctacaccg cttaaaatga
    14461 tagtttcgat tccacgccgg cgaagttgaa gatcaagaga agtaccaaaa aaagcacttg
    14521 gattgtattt tgtgatagtc aacatattat ctgtatcttt caaagaatcc ttcaaaagta
    14581 aatcagtgaa ttctggaggt acagaagcag tcattccacc catatcagtt tgttgactca
    14641 aataagggaa agtggagatg tctacgttca ctaaaacttt caaggcagcg gtacgtttca
    14701 atgttcttac taacaaatca ttgtttgtca gaatagatgc tgcagtgtgt ggagagagtg
    14761 taccgttatt tacgatctct ttttgcatgt cgatcgcgac aaaagcagtt tttgataaat
    14821 caaaaatcat attttccttt aaatcattca tgatgttcct tctttcaaat aacaatgttt
    14881 tttatgtaag taaatcataa atcgtttatt tgtcaactaa attaaaggat tcctttcttt
    14941 agtctagaga aattcagtgg taaaataaat aagtgtgaga aaacgcattc gatgcataga
    15001 tatagcgaga aatatcgtga aaatggagga atcagagtga agtatgtcga agtaaaaaat
    15061 gaatacaaac gatacaaaat gggagaaacg accattacag caaatgacga tatttctttt
    15121 tcaattgaaa aaggcgaatt agtgattata cttggtccaa gtggagcagg aaagtcaacg
    15181 attcttaata ttttaggtgg gatggataca cctgatgaag gtcagatcat tattgatgac
    15241 acagatattg cgcaattttc agataaacag cttactgctt accgacgaac agatgtaggg
    15301 ttcgttttcc aattctacaa tctcgttcct aatctaacag caaaagaaaa tgtggaattg
    15361 gcaacggaag tttctcctga tgcgcttgat cctgtagagg tattgaggca agtgggccta
    15421 gctcatcgat tgaataactt tccgtcccaa ttatcaggag gcgaacagca gagggtttca
    15481 attgctcgtg cactagctaa aaatcccaaa cttcttttat gtgatgaacc gacaggcgcg
    15541 ctggattttg aaacgggcaa gcaagtattg aaattattgc aaaacgctag cagacaacac
    15601 ggcaataccg ttttgatcat tacacataat tctgctttag cgcccatcgc tgatagagtc
    15661 attcacatta atgatgctaa agtaaggtct gttgaattaa atgatcatcc ttcatccatt
    15721 gatgagattg tatggtaggg ggattttatg aaaacaagaa catatcttaa agcaggtttt
    15781 cgcgaaattc ggcaatctaa aggacgattc attgcaatca tccttattgt catgctagga
    15841 acacttttat ttgtaggtgt caaaactgct ggacctgtca tgcaaaaaac gatggatcac
    15901 tatgttggta cagcaggact ttctgatttg caaatcgttt ctactggtgg cttgacacaa
    15961 aaagatattg ctcaagcaga aaagatccca gatgcaaaag tagaaacagg aaaacagatc
    16021 tattattcca atactggcaa aaacgaagtg attcaaatat tttcttataa caaaaataac
    16081 aagcagaaca agctacaatt aactgatgga aaactcccgg aaaaaccaaa ccagcttgta
    16141 ttggatgaaa aagcggaaga tgaagggtat aaaatcggtg atatttatca gattgattca
    16201 gatgaattga aagaaaaaga gtataccatc acgggctttg tccgttctcc attgttcatc
    16261 aacaatttgg agagaggata cgcgaacgtc ggaaatggta gcgtcgatta ttttgtctat
    16321 ttacctgaat ccgtttttaa aacagatatt tattctgtct tatatcttga ttttacaaac
    16381 gtaaagaatt tgaatacgta cagcaaagct tacaaagata aaatggagca aaatcaagaa
    16441 aaggcagaaa agtatttaca agatcgaccc gaagaacgct tagaagagct gaaaaaatcg
    16501 gccaacgaag aattagaacc ggcacagcaa aaaatagcgg atggcaaagc acaaacaacg
    16561 caagcaagaa cacggttaga agatgctaaa aaacagttag cgcaacagtc agcaactatc
    16621 cagcagcttc ctgaagcgca acgaacaatc gctgttcaaa cattatctca acaagaagct
    16681 caacttgctg aacaagaaag gcaattagtt gagaaagaac gtgaactggc ctctgcagag
    16741 gaagaattga atcgaaatgc agaaaaaatt gaaaatttaa ctaaaccatc atacctatac
    16801 aatgaacgtt cagaaaatgt cggttttcaa gaatttggtg atctgtctga tcgtatcgct
    16861 gcgattgcca atgtttttcc tgttttcttt ttctttattg ccgcattgat cacctttacg
    16921 acaatgacac gtatggtaga agaaaaccgc agggaaatcg ggacattaaa ggcgttaggt
    16981 tatacgaaag ttgaaatcgc aaaaaaatat gcaatctatg caagtctagc ctcagggata
    17041 ggtattgttt taggaacgat attaggaacg aatcttttgc caaggatcat ttttgagctg
    17101 tctaacgagc ggtatgatat tgggagtgcg cttatttttt atgactggcc gccgatttta
    17161 caagcagctg ccgcattctt cattgccgca tttggcgctg caatgatcgt tttattcaaa
    17221 gatttaagag aacggcctgc tgcattgctt caaccgaaag caccgaagcc agggaaacgc
    17281 atttttcttg aatacatcac tccgctgtgg tcacgcctta gctttaatca aaaaatcagt
    17341 taccgaaatc tcttccgata taagtcaaga atgcttatgg cgattatcgg aattgctgga
    17401 tgtgccgggc tgatggttgc aggcgtggga ttgaaagatt ctctaagttc tgtttcagcc
    17461 aaacaatttg gaccgatcat tgactatcaa gcaatcgtta cattggattc atccacggaa
    17521 gatagtcgtc aaaaagcagc ggatatgctg cagaagcaag aaaaagtaaa tgagatccta
    17581 gctattcata ctcaaaccgt agaattgaga aaaaaaggac atgcttcgca aagtctgaca
    17641 gtgatcgtac caagtaaaac agctgatttg gatcgtttta tccatttaac tgatcttgaa
    17701 gagaacccac gctccttgcc taaaacagga atcgctatta cgcaaaaagt agcagaacta
    17761 tttgacttat ctacaggaga tcatatttcg ctttatgatg aagatcaaaa agaatggaaa
    17821 gtcgaagtaa aagaaatcgt gaaaaactat ctaggtagct ttatttatat gtcccctgaa
    17881 tattatgctt cagttactga tcaatcgatg atggataatg catttttgct gcgtacagat
    17941 aagatgacaa caaaagaaga agaaatgctt tccgagaaac tgcttgccac agacgctgtg
    18001 acaaatacgt ctttcatgtc caaacaaatc gaaacgcaag aagaatcaat ggcgaactta
    18061 gactcggttg tcttaatctt tgtcgtatta tcaggactgc tggcttttat cgtcttgtat
    18121 aatctaacta atatcaatat ttccgagcgt gtacgcgaat tatctacaat aaaagtcctt
    18181 ggattttttg ataaagaagt cacgatgtac attgttcgag aaaatatcat ctttactctt
    18241 ttaggaatca tcggaggatt tggtgtagga tatatattga ctgacttcat cttgcaacaa
    18301 gcgtctatgg agaatgtcat ctttccatta gtgatcacat ggtgggctta tgtcttatca
    18361 gcaggattaa ctatcgtatt tacggttatt gtgatgattg ttacccattt caaattaaag
    18421 catatcgata tgattgatgc gttgaaatcg aatgaataaa aacagaggtt gtgaaatcag
    18481 cggtttgacc tgagtattag agaaacaaac ggcaaaatag tttttcatat tttgccgttt
    18541 tttgagctaa taccgaagga cagctgattg aacaccatga atcagaggtt gtaaaaaagc
    18601 cgtcctgcat cgagcaaaaa gaatactcaa ataaaattag ctcctcatgt tttacatttg
    18661 agaagactga ttgccgtaga taaaagcttt tgaacaccat ttatagaaga aacaagaata
    18721 ttttgctatt ttaaacaaga ggatagacaa atacatgtct gatctcttgt tttttattgg
    18781 gcaacagacc tcttagaatg tataaagata aattttacaa acgctttcgg ttcagttata
    18841 ataataaatg ataacttgta tatataagtt gtggtaggta ataaatcagt tgaatggagg
    18901 taaatatcca tgacatttaa agacaaagtc atttaccaaa tctatcccaa atcttacaaa
    18961 gacagtaatg gtgatggaat tggagattta aaaggaatca aagaaaaact cccgtatctg
    19021 aatgatctag gcatcgacat gatttggcta aaccctattt atccaagccc tcaaaaagac
    19081 aacggttatg atatctctga ttacacagcc atcgatccta tctttggtac gatggaggaa
    19141 ttcgaacagc taatagccga agcaaagaaa taccggatag aaatcatgct cgatatggtg
    19201 ctgaatcacg tttcgacaga acatgaatgg ttcaaaaaag cactagcagg ggaccagcat
    19261 tatcaggact tcttcatttt aagagaagag ccaacggatt gggtatcaaa atttggtgga
    19321 agtgcttggt cgccatttgg agatacgggg aaatattatt tgcacttata tgatcggaca
    19381 caagctgact tgaactggcg gaaccaagca gtaagagagg aactattcaa agttgttcgt
    19441 ttttggatgg aaaaaggggt aaaaggattc cgtttcgatg ttatcaatgt tatcggtaaa
    19501 gacgaacaat tgaaaaataa taaagaaaat gatggcaaac ctgaatatac ggacaaacca
    19561 atcacccata cgtatctaaa agaattgaac caagcaacgt ttgggacgga ttccactatc
    19621 atcacagtcg gtgaaatgag ctctacgacg atcgaaaatt gtatcttgta tacagaacct
    19681 agtagagaag aattatcaat ggtctttaat ttccatcatt tgaaagtcga ttatgaaaat
    19741 gggaataaat ggtctactcc tccatttgat tttgcagaat taaagcgact atttcataca
    19801 tggggagaaa agatgagtga gggtaacggc tggaatgctc tattttttaa taatcatgat
    19861 cagccacgtg cgctcaatcg atttgtagct atcgatcagt atcgaaaaca aggagcagag
    19921 atgctagcaa ccatgatcca tttgaaccgt gggacgccct atatttatat gggagaagaa
    19981 atcggcatga tcgacccaga ctattcatcg atttccgact atattgacgt agaaagtttg
    20041 aatgcttata agttactcct tgaacaagga ttcacagaaa aagaagcgtt tcgtcggatc
    20101 aaagcaaaat ctcgcgataa ctctcgaaca cccatgcaat ggtcagacac agaacaagca
    20161 ggatttacta cagggaaacc ttggttaaaa gttggaggca agctagaaga gattaatgta
    20221 gaaaaagaac tatcttctga agattctatt ttgtcctatt acaagaaatt gattcggctg
    20281 agaaaaacat atccaatcgt tgcacaagga gattatcatg cttatgaagc aaatcatccg
    20341 gaagtttatg gctttcttcg ccaatttgaa ggacagcagc tgcttgtatt aacaaatttc
    20401 tatgataaag atacaaggat cgccatccca gaaggattca cagaagccaa tattcttatc
    20461 gataattatt ccgatactgt tcttcatcaa agtctggagc taaaaccata ccaggcaatt
    20521 gctttgctga aaaattaaaa caaaaacaca aaacgtcgtt cctacgagct attctcgtaa
    20581 gatcgacgtt ttgtgtttta tttaaaataa tcttcttcag aaaatgaaga gttttcatac
    20641 tctcctttat cgccataaaa tttattgtag cgttgtttga atttacggaa aataaaagta
    20701 acgagtaatt tgactaacgt atagatagga ataccaaaaa taacgcccac aattcccatt
    20761 agatcaccca tgacaagtaa aacgattaag atcgtaatcg gatggatctg taaacgatca
    20821 cccattactc gaggaatcac caagtcaccg tggagaagtt gtacggcaaa ccagacaatg
    20881 acaaatttga cagccataaa agtagagtct tggaatgcaa tgatcaaacc tggaaagaag
    20941 gcgataaacg gcccaatata tggaatgata cataacaacc cagcagaaat agctaaaaca
    21001 ctagcaaatt ctaatccgat cagtaagaaa caaacccagt agactgctcc aaggataaat
    21061 gaagcaatga tttgcccctt tagaaatgaa cccaattgtt gattagcaat ctttgtcaaa
    21121 tttttaaaat ctgtacgaaa tgctggtggg acaatcgcta aaactgactg ataaaatttt
    21181 tgaggatctc tcaaaaggaa aaaagcgaca attggaccag taaataaagt aataaaaata
    21241 gctgtaacag ttgaaaaaat gttgctgatc cgttgtgcgc cactttccca ataatttcca
    21301 ataaatccag ctatatcaga cgtcacgtcg tcaatagaag cgaaaaattg attaaatgaa
    21361 tcggcaaaag gtgttttctc caaaaattgt cggagactct cttgaaaatc ttgaaacaaa
    21421 tctggcaaac tactgatcaa tgtctttgcc tgttcttcaa gaaaaggaat aagccatatg
    21481 cttccaagcc ccaataaaaa taaaataatc acataaacga taatgactga caagttcctc
    21541 gaaaggtgct tgctcaaacg tttgatgact ggttccaaaa gataatacaa gatcagtgca
    21601 aacaataatg ggggcaagat agtagcgata atgattccga caggcttgaa tataaacgag
    21661 atctgatgga aaaaataaat gatcagcgca agtaagataa ctattcctaa aacatagtaa
    21721 gaggtttttc ctcctaaaaa gcggatcaaa cgattcgttg tatctgtttc ttttttcact
    21781 ctttttcctc ctttatctat aacttcatta tagacaaaag cgggtataaa aggaaaatga
    21841 tagaggttga ttgcaaaaat atcgtcaatc attcatataa actttctcat caattaaaat
    21901 aagaaaacgc ttgacagttc ttttaaccat atgataaatt aaatcctgtt gctaaaacgt
    21961 aactattacg gtttgcgaaa agcgaatcgg aacattcaaa tgaaagaaga tgagaagagg
    22021 agtgtcataa atgaaacgat tgataggtat attggccatg ctggttttag caggtatgtt
    22081 aactgcttgc ggggcaagtg ggaaagccga ggacagtaaa gaaaaattga gcgtgatgac
    22141 gaccttttat cccatgtatg attttacaaa agcaatcgtc ggggatgaag gagaagtaga
    22201 gttattgatt ccagcaggga ctgattccca cgattatgaa ccttctgcta aagacatggc
    22261 aaagatccaa gatacagata tctttgttta taatgatgaa aacatggaaa catgggtccc
    22321 cgcaatccaa aaaacattac aagaaggcaa tgtccatacg ataaaagcga cagaaggtat
    22381 gcttttactt ccgggaagtg aagaaggaca tgatcatgac catgaacacg gggaagaagg
    22441 ccatacacat gagctagacc cacatgtatg gctagctcca agtttagcaa tcaagcaggt
    22501 agcaaacata cgtgatcagc tgattgaagc ttacccagaa aaacaggaag tatggacaaa
    22561 aaatgcagca gcctataccg agaaacttca agcactccat cagctttatc aagaaacctt
    22621 taagcaagca aaacaaagat cttttgtcac gcaacatgct gcattcaact atctagcgtt
    22681 agaatatgga ttgaatcaag tatcgattgc tggtctttct tctagtgaag aaccgtctgc
    22741 ggcgagaatt gctgaactga aacactttgt taaagaacat ggaatcaact atatttattt
    22801 tgaagaaaac gccaaagata gtattgccag aacgttagca aacgaagcag gtgtatcttt
    22861 agaagtattg aatcctttgg aaggattgac gaatgaacaa atagaaaatg gagaaaacta
    22921 tctttctatc atggaagcaa acctcgaagc gttgaaaaaa acgacagaga cagaaaaccc
    22981 gctagaagat actttgaccc ccaaaaaaga aaagagcgtc tacaacggtt attttgaaga
    23041 cgaggatgta caagatcgct ctctttcaga ttgggcagga acttggcaaa cagtagatac
    23101 gtatgtagaa gatgggactt ttgatcctgt ttttgaatat aaagaaaaat tgaatcagga
    23161 taagacagca caagaatata aagaatatta tacgaagggc tatcaaacag atattgcttc
    23221 aatcaagatc agtgagaaag agatggcatt tacgtttaag aatggaacaa cgaaacgttc
    23281 aaattatcgt tatgtaggga aagaaatctt gacctacgag gcaggaaacc gtggcgtaag
    23341 gtatctattc gaagcagaag ataaggaaag tggcgctttt agatatattc aattcagtga
    23401 tcacacgatt gcaccggctg actccgaaca ttttcattta tatgcaggaa
//
LOCUS       DACCYH010000042        22953 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.042, whole genome shotgun sequence.
ACCESSION   DACCYH010000042 DACCYH010000000
VERSION     DACCYH010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.042"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..132)
                     /locus_tag="IVY85_001568"
     CDS             complement(<1..132)
                     /locus_tag="IVY85_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8044590.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..841
                     /locus_tag="IVY85_001569"
     CDS             542..841
                     /locus_tag="IVY85_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8044591.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            914..2275
                     /locus_tag="IVY85_001570"
     CDS             914..2275
                     /locus_tag="IVY85_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP8044592.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            2297..2557
                     /locus_tag="IVY85_001571"
     CDS             2297..2557
                     /locus_tag="IVY85_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044593.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2571..3323
                     /locus_tag="IVY85_001572"
     CDS             2571..3323
                     /locus_tag="IVY85_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP8044594.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(3417..4022)
                     /locus_tag="IVY85_001573"
     CDS             complement(3417..4022)
                     /locus_tag="IVY85_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8044595.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(4075..5049)
                     /locus_tag="IVY85_001574"
     CDS             complement(4075..5049)
                     /locus_tag="IVY85_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP8044596.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(5427..6206)
                     /locus_tag="IVY85_001575"
     CDS             complement(5427..6206)
                     /locus_tag="IVY85_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8044597.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(6273..7544)
                     /gene="celB"
                     /locus_tag="IVY85_001576"
     CDS             complement(6273..7544)
                     /gene="celB"
                     /locus_tag="IVY85_001576"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8044598.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7697..9094
                     /locus_tag="IVY85_001577"
     CDS             7697..9094
                     /locus_tag="IVY85_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8044599.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            9175..9615
                     /locus_tag="IVY85_001578"
     CDS             9175..9615
                     /locus_tag="IVY85_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8044600.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9763..10089
                     /locus_tag="IVY85_001579"
     CDS             9763..10089
                     /locus_tag="IVY85_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8044601.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            10090..10839
                     /locus_tag="IVY85_001580"
     CDS             10090..10839
                     /locus_tag="IVY85_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044602.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10919..11374)
                     /locus_tag="IVY85_001581"
     CDS             complement(10919..11374)
                     /locus_tag="IVY85_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8044603.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11801..12076)
                     /locus_tag="IVY85_001582"
     CDS             complement(11801..12076)
                     /locus_tag="IVY85_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044604.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            12370..13185
                     /gene="thiD"
                     /locus_tag="IVY85_001583"
     CDS             12370..13185
                     /gene="thiD"
                     /locus_tag="IVY85_001583"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP8044605.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(13514..14965)
                     /gene="cls"
                     /locus_tag="IVY85_001584"
     CDS             complement(13514..14965)
                     /gene="cls"
                     /locus_tag="IVY85_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8044606.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            15306..17972
                     /locus_tag="IVY85_001585"
     CDS             15306..17972
                     /locus_tag="IVY85_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8044607.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            18336..19088
                     /locus_tag="IVY85_001586"
     CDS             18336..19088
                     /locus_tag="IVY85_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8044608.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(19110..20264)
                     /locus_tag="IVY85_001587"
     CDS             complement(19110..20264)
                     /locus_tag="IVY85_001587"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP8044609.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            20449..22890
                     /locus_tag="IVY85_001588"
     CDS             20449..22890
                     /locus_tag="IVY85_001588"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP8044610.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         7392 a         4027 c         4772 g         6762 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaagaaaa
      541 aatgaaaaat cggttaattg tcgcatgtgg aagtggagta gcaacgagtc agacaatcgc
      601 tagtaagatc caaagtatgt tagaagatga cggcatcgca tttcctgtag aagcagttga
      661 ttacaaatcg attcaaaatg agcttctaac tgctggaatc tatgtgtatg tcgcacaacc
      721 tgatgaggaa gtacttgaac aagcaaaaga tttgggtatt gaagtatttc caggaattcc
      781 atttttaact ggaatgggtg tagaacctat ttatgacagt atcaaagagt tagtacagta
      841 aaggaggatt gttggataaa aaagttggtt catagtttta tttactatat ttagaaagaa
      901 agaaggttta tttatggaag ttttccaaac cattatcaat tatgttttga acttaggatc
      961 agccattttt gttcccctga ttattttgat acttggattg ttggcaggga tgaaattaaa
     1021 aaaagcattc atgtctgctt tgactttagg gattgctttt agcggaatgt ctatggtcat
     1081 tggctatatg tctaacgcag taagtccagc atcagaagca ttggctaaaa atacgggaat
     1141 cagtttgcct gcacttgatc ttgggtggac aggtgcagca agcatcacat ggtcgtggag
     1201 ttatgctttt atattttttg cagtaacgat tggggtcaat tttgtactgt tactttttaa
     1261 ttggacgaag acgttaaatg ttgatatgtg gaatgtttgg gggaaagctt taactgctta
     1321 tttagtttat tatgtttccg gtagtttggc tgcaggcttt cttactgcaa tggtccaagt
     1381 tatattggaa ttaaagcttg gtgatatgtt tcaaaaacat atacaagatc tcacagggat
     1441 tccattagta acagtgactc attttatgac atctgccgct gtcttgttgc tgcctttcaa
     1501 tatgatcatg gataaaattc ctgctttaaa taaacgagcg gatacgaatg cattgaagaa
     1561 gaaaattggg atcttctctg aaaactctgt aatgggattc attattggat tggggcttgg
     1621 ctttgctgct gcgtatggcg tttcaggttc tcttaatcta gcgatccaaa cagctacagc
     1681 attaagtttg tttcctatga tctctaaaat gttcatgcaa tctttgtctc ctctagcaga
     1741 cgctatgtca gaatttatga aaaagcggtt taaggatcgt gaagtatata tcggattgga
     1801 ctggccgata ttagctggtc gttcagaaat ttgggttact gctattttac ttgttcctgt
     1861 gtttattggt tatgccatta ttttgccagg aaaccaagta ttgccattag cagggattat
     1921 caactattcg attgcggtcg gcggattact tttaacagga ggaaacttgg ctcgtatgct
     1981 gatcttggga atcatcacta tgcctattta tttgtacggg gccacttacc ttgcgccaat
     2041 actcagtgat ttagcagaaa aaagcggagc agtcgaggga atcaagaaag gacagttgat
     2101 cacatggtca tccatagaag ggccggagtt cagggtttta tttgcggagg catttaatag
     2161 gaatattctc gctattgtag gatgcgtgat ttttctagga ctgtttgttc tactttataa
     2221 atatatgata aaagcgaaag taccgagtga acgttatgct gaactacaaa aatagcattg
     2281 aaatggtggg aaagtaatga aaagagaaga gacagataaa ataaaatgga cagtcgctct
     2341 ttgtggtaca cttctgctat ttctatatgg actctttact cagaatatca tcatcaatct
     2401 attggtcatt tttttcgcac tagtgatcta taaatatgga aatcacgttt tattcagaga
     2461 gtacgatgaa aaaagaaaac ggaaaataga agaatcaatg aaaataaaag aagctaccaa
     2521 agaaatattg agagaaaaaa gttttataaa aagataatgg aggggtcatt atgtcagatg
     2581 gacgtatgtt atttgagaga gaacgacagg acatggcacg tatcgtaaga gtgattttcg
     2641 aaaggaaaaa cacaaatgtc gcaggaggga atttttcctt taaaaccact gatgatcaag
     2701 ggaaagacta catcatcatg acgccgacca tgatgtcaga agcctatcta ggagagattt
     2761 ctcctgcaca ggttctagta gtagaaccaa gaacgcgtag aatcgttgac ggagtcggtc
     2821 atttaactcg tgaaatcaat atgcatgaag cgatgtatga gactaatcca gctatcaaag
     2881 ctgtcctaca tgctcatgcg cctaatgcaa tgttctgggc gactactggg ttgcctatgc
     2941 ctaatttgac agaagccaca gctaaagtaa aagagattcc tgtcatggac tttgagccta
     3001 attgctctga agaacttgct gaattgacaa gcgactatgt aaaaaaacag catctcgaat
     3061 taccgcatat gttgctgctc aactctcatg gcgtattgat caacacaggt ggagaatcag
     3121 gaatcgaagc tatccataaa gcactgcaaa tccttgatac agttgaatgg aatgcagaaa
     3181 ttgcctacaa gcagacgatt tttcaagcat tagggattac tgacggtttt tattcaaaag
     3241 gtgagaaaat cgggggattg gaagagttga aacataaaac ggctatttat aataaatctg
     3301 ttattgcagc tggtggagac taacctatct gacacaaaaa actacgactt tcacaaaatc
     3361 tgatccagat cgatatctgc ttcaggttgt attatgtaag tcgtagttct tttcttttat
     3421 ttagttaaga ggtggtctgt ccattccctc gcttgcttga tttcgtccat cgtcaactga
     3481 tgacctgtta cagttagctg aatagtcaca tttgcttggc gtgtttcgaa ttgatcggcc
     3541 aattctttaa aggaagcagg ggaaacgatt ggatcgttat caccataaga aatccagacg
     3601 gttttatcct ctaatgtgga acggctttga tgaacaccta gagacatcgg atggaaaaac
     3661 aaccctttat ttagctcggt ctcacgctcc aataataaat gagcagcgat gtttgcgcca
     3721 tttgaatagc cgacaagcag ccagtcactt aacggtatca gttctttttc gctgatttct
     3781 ttgatttttt catggagttt atctgtttct ttttccaaac tgtccaggtc gaattgattc
     3841 aatccattac gcttgaaaaa acgattcatc ccatcttctt ggaccgtgcc acgaaaagaa
     3901 agcaatgtac tgtttggtgc aagaaagtga gcgatatcca gtaaagaatg ttcatcgcca
     3961 cctgtgccat gaagtaagat caaatgcttg ccacttgttc ctttttcaaa aatgtaggtc
     4021 attttgttcc ctcttttctg tttctataga tgcaaatgaa gtagcatgaa gagattattc
     4081 acgtttttta tttgcatcac ttgtatcgaa aggacggaca taagcttcaa tggcttcacg
     4141 acgttcttct aagaatggag gaagagatag tttttctcct gctgattctg ctggttcatc
     4201 ttcaaggaat ccaggaccat cagttgctaa ttcaaacagt acgtgatgag cggctaggaa
     4261 gtactcagat tggaaataga aacgatcgac gaaaccagaa tttggcaatt caatcttatt
     4321 gattacttga atccagtatt ccaatgcttc tttgtctgct acacgcaatg ctaagtgatg
     4381 gatatttcca aatccttcga ttgcttttgg caagtctgtc cgatgttcaa taatgatgct
     4441 tgcaccattt cccccttcac cgatttcaaa taagtagaaa tctcctgaat ggtctgtttc
     4501 tttgaatcct aagatttttt caagaatcaa gcggatatgc tctaattgac tgacacggac
     4561 aaagacaggt ccaagtcctg tgatcgcaaa ctctgtcggt acattgctgt ttttccaagg
     4621 aatgccagct gctacacctt gattgttttc gtcagagatc aattgataat actgattatc
     4681 gaaatcttca aattcgatat attttttgcc gaaacgttca ttgattgctg aatgagtaac
     4741 gtcatatttt tcaaaacgtt ccaaccaaaa gtctaatgct gcgtcacttg gcacacggaa
     4801 agaagcacga gagatcgtat ctgttccttt ttttcctttt ggctgatttg gaaaatcaaa
     4861 gaaagtcata tctgttcctg gcgcaccagt atcgtctgta aagtaaagat ggtaagtttc
     4921 gatgtcatct tggttaactg tttttttgat taagcgcatt cctaaaatat ctgtaaaaaa
     4981 gcggtagatt tttgtagcgc tagatgtcat tgctgtgacg tggtgaattc ctctgatttg
     5041 tgtatccatg ataaatttcc tcctgttaac ttacaaaaag taagtagttg atttagacat
     5101 agtatagagg gtgcatctct attttgcaat taatttgtct tctaaagtat caggaaggaa
     5161 tcaagaatgg aaaagaggag ggacggatca ataaaataaa aagacttacg actagctgta
     5221 tcgatttcaa aaaaactagc tttaaaaagc taactttaga atcattacgg gtggtcataa
     5281 gtctttttat gctgagttat tttttcattt ttgagctaat gcttaggata gaatagaatg
     5341 ttgatttcac gatctcttct tgttacttga aactgcttac cactgtcacg atctcttggt
     5401 aaacggcttt ttcacaacct ctttggttaa gttgaacgca catccagcac agaatagttc
     5461 cgaacatcaa atcggtttcg gctggaagaa tattcaatcg gctgtccatc tttgagatag
     5521 acgacttgct gaacttccag tacaggatcg ttttctgcac agttcaaata ttgctgatca
     5581 tattcatcac ttttatctgc agaaatagca cgataagccc ctgcaaaatg gacattcaat
     5641 tcttgatgaa gatgatcata gatagaggct aacaaatgtt ctttttttaa acccggcaca
     5701 agatggactg gcataaaggt atgctctaaa acatatggct cgccatctaa gatccttaac
     5761 cggtgaatat tgtagacagg ttgctcggca tcgatcatca atcgttcttg gatatattga
     5821 ttagggaatt caacatcaaa atgaatgatt tgactcttca aagaacgatg tctgcgtttc
     5881 atttggagac tcaaaccttc atactcgtta gcgggcgatg tgtctttttg cataaatgga
     5941 tgattcaaga ttttcgttcc tgatcctcgc tgcgaataga caagtccttc cattgctaag
     6001 atattgatgg cttttttgat tgtcatccgg ctcgcaccaa attcttttac caagtcgact
     6061 tgattaggca aaaaactatc gagaggatac tcttttgaac ggatacgttc tcttaaaata
     6121 tttgcgattt cttcgtattt tggcacgtta ttcacctctt ctagagtata acgaaagatt
     6181 atgcttccga caactcgaag tagcatctgg aacattgttt attcgaaaaa agaggtcatg
     6241 acaagttttt gtcacaaccc cttaatgatc tattaagcaa tatctaaatc tggttgttta
     6301 tctagtactt tcaggaatgg gtaccagata aaggcaacga tgacaaagtc gatgacttgc
     6361 agcataccac caagccatga atttgttgct aagacaccgc ttgcaatgat tggcacagtc
     6421 catggaacag agacgccagt tggtgcaggg acaagtccag cagccattac caaataattg
     6481 aacgtcgtta cgattagagg tgccacaacc caaggaataa ggattgttgc attcaatacg
     6541 atcggtagac cgaagatcgc tggttcattt acattgaaaa taccaggtgc taaagctaat
     6601 cgtccaacat cacgatactg tttcttttta agaacaaagg ccatcaataa aacgacagct
     6661 aaagtcattc cagagccacc aataccaaca gtgaaggttt ccatgaatgg ttttgtgatg
     6721 atgtgtggta attcttttcc atgttgataa gcttctaagt tatcaagcat cagtgtattc
     6781 caaatcgggt ccattacaga gttgacgatg atttgtccgt gaagtccgaa gaaccataag
     6841 aattggacaa agaacaaagc aatcaatgtt gcaggtaaac tacttcccaa accagttaat
     6901 ggtttttgga tcacttcata aacgacgtca tgtaagttag cattgaaaat accagtcatc
     6961 acagcattga ttactaagaa aatagttagt gtcactacag cagggatcaa agcggcaaat
     7021 gattttgtta ctgcaggcgg aactccatca ggcattttga tctgccagcc gcgtttcgtg
     7081 attctgcagt agatttctcc agccaagaaa gcagcgatca ttccaatgaa cattcctttt
     7141 gcgccaagac gatcaagtga aagcacacca gtcacatctt caccgccagc ggttttcatt
     7201 gcaaaaggcg taagaatcaa gaagctggca aaagacacgg caccaccgaa aataccttca
     7261 acgtcataag attttgataa ataatagccg ataccaaaag taacaaaaac cgacatgatg
     7321 ctcattgttg cattttgccc attaccgaat aaattcgata aagttccctt tgttgcgtca
     7381 ctgaaaaaag gcaagttatt gataacgaca acgatcgaac cgaacatcgt taaagggaag
     7441 gaaagcatga aagcatcccg cagcactgta aggtaacgat tttgccctaa taaattagct
     7501 aagggcatga ttttctcaga aagcttgtcc ataaaaccgt tcatttttat tcacctcaaa
     7561 tttatttgca atcgattaca tacaaagtat aacgccaaaa attttaaatg tctagtcatt
     7621 ttcataaaaa gtattgattt attttttagg gttcgttatg attacggtat caagaaaaaa
     7681 gagaggaagt aaaaaaatga aaacctacac attcccagaa aatttttggt ggggttctgc
     7741 tgctagcgga ccgcaaacag aaggaagagt aagtgatgat ggtaaaggag aaaatatttg
     7801 ggatcattgg tatgaattag agcctgaaaa attcttcgat caagtaggac ctgaaaaaac
     7861 ttcgcaagtt tacacaaaat acaaagaaga tgttcagtta atgaaagaaa ctggccataa
     7921 cacatttcgt acatctatcc aatggagtca tttgattcca gaagggacag gtgaaatcaa
     7981 tcaaaaagct gtttcttttt atcgagatta ttttactgaa ttgctggata atggtattga
     8041 accttttgtg aacctttacc atttcgatat gccactacca ctgcaagaaa aaggcggatg
     8101 gttatcaaga gaaacggtcg acgcttatgt ttcttatgca gcagcgtgct ttgaactgtt
     8161 tggagatctg gtcaaaaaat ggtttactca taatgaacca atcgtacctg tagaaggagg
     8221 ctacttgtat caattccact atccaaatca tatcaatatg aaagaagcgg tccaagtagg
     8281 ctatcatgag aatctagcaa gcgcaaaggt aatcaaagcc taccatgaag gcggacatga
     8341 tggtgagatc ggaatcatct tgaatctgac accaagctat ccgagagatg aaacgaaccc
     8401 agaagatgta aaagcggcga agatcgctga cgcattcttc aaccgatcat tccttgaccc
     8461 tgcagtgaaa ggagagtttc cacaagagtt gatcgaaatt gtcaaagaat tggctataat
     8521 gccagagatc catgaaggag acctagaact catccgtgaa aacacgattg acctgctagg
     8581 aatcaattac tatcagcctc gtcgaatcaa agcaaaagaa actccaatca atcacgaaca
     8641 tagccccatg ccagatgatt attttgataa ttacgacatg cccaataaaa aaatgaatcc
     8701 atatcgtgga tgggaaattt acgaaaaagg aatctatgat attttaacga atacaagaga
     8761 aaactatggt aatatcaaat gctatatttc tgaaaatggg atgggggtcg aaggagaaga
     8821 acgatttgtt aacagcgaag gaattattga agatgattac cggatcgagt ttgttacgga
     8881 tcatttgaaa tacgtccatc aagcaatcca agaaggaacc aattgtgtgg gttaccacat
     8941 gtggacatgt atggacaact ggtcttggac aaatgcttat aagaatcgct atggctttat
     9001 cgctgttgat ttggcacaag acggtaaacg gacgatcaaa aaatcaggtc gctggtttaa
     9061 aacggtctct gaaaaaaacg gatttgatgc ctgatatttt ttgataaaac gttcacaaaa
     9121 tgtttgttct aacactgtaa tcgtgctaca ctaacagtga aggaggcgtt tacaatggta
     9181 gatacttatc gtaatatttt agtagcaatt gatggttctg aaaaggcaga aaaagcattt
     9241 cttgaggcaa tcacgattgc caaacgaaat caggcaacgt tgcacatcct gtatgtcaat
     9301 gaagtaacag gcaattactt tggtgatttt gcatttgtta caacaagcct tcaggaagaa
     9361 ttggatgagg ttgcggaaaa ccagatgaaa gaacatcgaa acttggcaat tgaaaaaggc
     9421 ctgacagata tcgagacata tgttctttac ggttatccaa agacactgat tgctaatttc
     9481 catgaatcga aagaaccaat cgatctgatt gtcatggggg cgacaggtct gaatgcggta
     9541 gaacgtgcac tagtaggttc cactacttcc tatgtagtca accacgcatc atgtaacgtc
     9601 atggttgtaa aataaataaa ttaagggaac agaaagcaaa aaagcataac gcttcttttg
     9661 ctttctgttc cttttatttt tttgtaaaaa attactttac ctgatgaagt aatcatgcta
     9721 ttctaattac aacgacagat ggagtaatat ggaggaaaga aaatgatttc aagtgatgga
     9781 atacgcggat acaatgatgc gattatttta tcgatcctta acaaaaacga ttcttatgga
     9841 tatgaaattt ctaaaacgat tcgtgagagg acaaatgatt cctatgtgat caaagagaca
     9901 acactttact ccgtttttgc ccgtttagaa aagaatcaac tgattgtttc gtatcctgga
     9961 gaagtgactc atggaaaaaa gcggacatat tataaaatca ctgaaatagg gcgtaatctt
    10021 ttagaagaaa aaaagcaaga gtggctgacg acgaaagacg tagtcgatca atttttggag
    10081 ggaaattaaa tggatacaat ccgtaattat ttagacagtc tttttatcgg agtacctcaa
    10141 agtaccgaga tagataagct gaaaactgat ttgttggcaa atatggaaga ccattatcat
    10201 gaattgatgg gagaaggaaa aaatgaacag gaggccatcg gtacagtaat ttcgacattt
    10261 ggttcaatcg atgaattatt agaagaacta gatgtcgaaa aaaaacatca agctgacgag
    10321 acggaaacaa acacagcttc tatttattta agcgaagcag aaaactactg gaaagaatac
    10381 agagcggcaa gtctccaagt ggcatcaggt gttctattca ttagcttatc ttttgcaagt
    10441 tttcttttct tttgttcagc aggatatgtg ttcatgggta tttcttgtct gattttcggt
    10501 atagcattag ctgtcggttt ttttattgct tctggtatga aaatcacacg tttaaatcac
    10561 tttttgcacc atcggaaaat acctgaaaaa gtattagcag aagcaaaaga aaaagaagaa
    10621 gagtatcagc gaagttttgg cttctcactg attgcaggaa ttggattatg tattttttca
    10681 cttttcccac ttttggcttc tttaatgtgg tatatggatg gtagtatcgg tgcctctata
    10741 ttttttgtta cggtaggaac gggtgtcttc ttgatcattt atggcagtct agtccgtcat
    10801 tcttatcgtc aattcactca aagcgcttat tactggtgaa tgtattcaga tttgtaaaaa
    10861 tattctattt atccggcgta gaaaaaaaga ctgtgacacg cttttgtcac agtctttttt
    10921 atatataata atttaaaaat tcatcatcta aatccgtaga attttctaga ttgcttaccc
    10981 atgtaaaatc gttatagaca gatttcagtt gacttctgcg ccattgtaca gggataaaaa
    11041 gacgcatgct atcttcattg aatccgacta ataatttctt tccgtcagta atgatgggac
    11101 gttgtaacaa agtaaaatgc gagacgatca gatcgacaag ctctgaggtc gtacattggt
    11161 cgatttggtc tttcaaattt ttataatcaa tcgagcgttt agctaataga tcgtcaaatc
    11221 cgttttctgt taatgacaat aaataaaata tctctttcct tgttaaaggc tgtttaggtg
    11281 tgaaaattcg ttctctgtaa ggcatttgat ggtctttcag ccagtttgcc atcagatggt
    11341 aactatgtgt gcgagaagaa tagatcgtaa gcataaaaaa actccttaaa tagtccatta
    11401 atttgttaga tctgttgaga taaataatag gtaaggagct gtgcataagc attgatcaaa
    11461 acaacccaaa catcattaga aataactttc taaagtctta aacttgcgac gagaagcaac
    11521 tagactaata gaaaaaatgt cttagccatt tggcttcctg tttgtttttt tgcttatgct
    11581 gcagctcctc gggaaagggt tctaacaatt tgaagaaggt tgatgattga tctagtttaa
    11641 attgtgtgaa atttgaaatc tggtgttttt actagatgaa tcacctaaca actatgatta
    11701 taacttatag aacttctgtt ttgcattcgg ttttgttata gttgttataa agttttattt
    11761 tattacagaa catttttaat taataaagaa cagttagaag ttaaaattgc tgattcacat
    11821 agagtttatg acttcttgga aattcttttt caatcagttt tttcatctgt ttatgaaatt
    11881 cttttttttc agtaaacata aaggtttggg tatcgtaacc ttcttcatgt aggaaataga
    11941 ttattccttt ttctgtgttt tgcgtataag cttttatact cctgtctttt ttgaaagaac
    12001 agatcgtata gccatctatt tggtctggta gtcgtttgat tttcttcaaa atgacttttt
    12061 tatcctcaag tttcattttt ttcacctgac tttatttttt tcatatggaa atcatagcgc
    12121 ctttggttat atgaaaacaa gaaggatact gaaatatata taaatcaaat agtggttatt
    12181 tattattaga atagcgaata caaataaacg accactattt tctgatttat tgcaaatgtt
    12241 ttttcaaata aatttgatta ccaagaaaag gtagtggtat atttactgag ggatactttg
    12301 aaaaacatat gatagaaaca gtaacagtat tatgaaaaat ctttttataa aaatggagga
    12361 atcgatttga tggaagatca gtatgtacaa gcaatgacga tcgcaggatc agatagcgat
    12421 ggaagcgcag ggatgcaagc agatatgcat acattcttta caagaaatgt ttacggtgtt
    12481 tctgtcatga ccgcatgtgt tgcaggtaat tcttacggaa tcggagcaag tgtgacattg
    12541 ccaacagatt ttatcgacaa agaatttgaa ctgattgcga aggactttca aatacgtgca
    12601 gctaaaacag gtatgctagc agattcaaag ttgattgaga cagttgtaaa aaattaccaa
    12661 aaatatgatt ttggaccatt agtcgtggat cccgtaatcg taacgaaaca tggaaatctt
    12721 ttactagaag aatctgcttt gcaaagcttg aaagaaaagc ttgtcccttt ggccgaagta
    12781 ctgacaccta atttttatga agcagaaaag ctgactggac ggacattagc agctgaagaa
    12841 gattttgtgc aagcagcaaa agaactgcaa gatatgggtg caaaaaacgt tatgatcaaa
    12901 ggacatcatg ctaaaaatgc gcagcaagaa acagtcaatg actacgtatt gttcgaatca
    12961 ggaaaatcat tttggttaaa tgcaccattt tatcctaccg accggatcaa cggaactggc
    13021 gattcgctat ctgcatgtat tgcagctgag ttagcgaaag gaaaaacagt tgaacaagcg
    13081 atccgattgg caaaagacta tgtaaaccgt gcaattcgtg aagaaataaa tgtagggcac
    13141 aaatatggcc ccatcaatca ttgggcagct ggtgaattag agtaaaagaa agaagttgtg
    13201 aaaaagttgc cctgcatcaa gtaataagaa cagccaagta aaaacagctt cttatgtttt
    13261 taacttggtt ggacttatta ccgcagatgc aagcttttga acaccgttta agaaagaggt
    13321 cgtgagcctg ccgtccagct ccgagtcaca aggaacaagt tcaaaaaaca gcttctcatg
    13381 tttgttgaat attgtgactt gtgaccgagg agttggcagg agaacaccgt ttgttcggaa
    13441 gaagggactg tgacaagatt tttgtcacag tccctttaat cttagtatat ttttctctac
    13501 ttgaataaaa aaattatagg attggagaaa ataatcgaga aatttgttgc ttgaataata
    13561 gccagttaga catttccttg actgtttcag ttgtcatgga tgaacaatgt gtctcatctt
    13621 ctttgaactg agtagcaagt tcttctaaaa actcaggatc gtaaatgaca gcacttgttt
    13681 caaaattcaa tcggtagcta cgaatatctt gatttgctga acctaccatg cagatttcat
    13741 catccattat caatgttttt gcatggagga agccgttctc atagacaagg atttgtacat
    13801 tttccttgat cagctgccga gcataatatt gtgttgctcg ataaataaaa ggatgatccg
    13861 gtttgttggg aatcatgatt ttcacatcta caccagaggc agttgcgatt tttaaagcag
    13921 caatgacact ttcatcagga acaaggtaag gtgtctgtat ccaaacacgt tttttagcag
    13981 aagtaatcaa tttgataaat gaaagcttga tttgttccct ttcgttattc ggtccgcttg
    14041 aaacaagctg gatggatgta tcagcaagct tagattcatc tcgatctgct tttttaaaga
    14101 aataattcaa atgatagccg accttttttt cttcaggtac cgagacgttc cagtctgtta
    14161 agaagcgaag ctggagcaaa gaagaagcgg caccgaaaat ccgtatatgc gtatcgcgcc
    14221 aatagccgaa tttttttgtt gtatttacat attgattggc aatattgaaa ccaccggtat
    14281 aactaatctt tccatcgatc acaacgattt ttcggtgatc atggtaattc aatcggaaac
    14341 gaagtaatgc cctttgtgag gtaacaaatg tatggacatg tccaccattt ttgattagac
    14401 gattgaaatc ttttgctttt gtgccatgag agccaaatgc atcatataat attcgaactt
    14461 ccacgccttc tgcggctttt tcttctaaaa catgtaagac ttgctggccg atattatctg
    14521 tcacaaatgc gtaatattcg atatggatcg aatgtttggc tttttttaga tcttgaagca
    14581 gtgaatccaa tttctcttgt ccgtctgtga gaagagtgac agaattcatt cttgtcagcg
    14641 gcatacgatt taatgaagag aagaagtcaa caaattgctg tttgtcttta tcgcccatgt
    14701 caggatcgta atgttcaatg gtatctcctc taaaagactg aaagttttct aattctttca
    14761 agtcactttg tcggagatag aattttttct tatccgttaa tccacgtccg aaaaataaat
    14821 ataatacaaa gcccacccca ggaagggcaa acaatactaa gagccatgcc caaatggctg
    14881 ctacatctcg gggtttgatc aaaatagcca ccaaagcaat aagtgcattt aatagataaa
    14941 gggcggttat aatactagat accacaaaaa tgttcctcct tacatacaat aacattagta
    15001 tagcatgata gaagaaagaa gcctaccaag gtgaatatac ttttcactcc tttcatgaaa
    15061 atgtaggtaa atcgggctaa agtagttgct ttttgtaggt taataaggta ggatattagt
    15121 acagttgaat atctccttag gggagtaacc ggcaacgttt gttgcgataa tatcaacagt
    15181 aagttaacca aatggttttc tggtattgta tgaaatggtg agacctaaga caaatgcgta
    15241 tttgtcttag gtctcttttt tttcagacag gactagggcc aagatcaaaa aatgggggat
    15301 gaaatttgga aaacaaggaa caagcacaac aagcggtcta taaagaaacg atcgactcgt
    15361 tgatgcagca aacgcagagc tcaaccgatg gattgagcac cacgcaagcg aaggaaaaat
    15421 tagaaagaga tggcttgaac cagctaaaag aaaagccgaa gaagccaact tggaaattgt
    15481 tcttagaaac tttcaaagat gcaatggtta tcgtattgct gattgtcgca ctgattcaga
    15541 tggtcatggg tgactacatc gaattcttag ttatttttgc agtattgatg ttgaattcta
    15601 taatcagtgt tgtccaaaca aaaaaagcag aaggttcttt agaagcgttg aaaaaccttt
    15661 cagcgcccga tgctcgagtg attcgtgatg gtaaggaaaa aacgattccg gcaaacgaac
    15721 ttgtggtagg agatatcgtg ttgttagacg caggcgatta tgtacctgct gatggacgtt
    15781 tactagatgc gggatcatta aaagtagatg aaggtatgct gacaggagag tcgacttctg
    15841 cagaaaaaga agtaacggat atcacaagta cggtaccaat cggagatcgt acaaatatgg
    15901 tattcagtgg aacgatcgta acttacggac gcggtaaatt cttagtgact tcaactggga
    15961 ataatacgga gatcggacaa gttgctggcc ttttggaatc aacaactgag caagtcactc
    16021 cgttacaacg aggtctggat aaattcagta aaaaactaag tttggctatt ttggcattgt
    16081 ctttattgat tttaggtatc caactgtttc gtatttatct tggagaaggt acaggagata
    16141 tgactgcaga tatcgtcagc gcagtgatgt ttgccgtagc cgtagccgta gctgctattc
    16201 cagaagcatt acagtctatc gtaacaatcg tgttatcttt gggaacgaat aaaatggcaa
    16261 aacgtcatgc cattattcgg aagcttccag cagttgaaac acttggttcg acaagtatca
    16321 tctgtacaga taagactggt acattgacac aaaacaaaat gacagttgtt gactactttt
    16381 taggtaatgg cgatactggg gatttcacaa atgacccttc aaaatggaca gctgacgaac
    16441 gtcgcttgat cgaaatcagt gttttagcga atgatgcatc gatcagtgaa gacggtacta
    16501 aattgggcga ccctacggaa gtagcgttta tcgactttag tgaaaaatta aaccagcctt
    16561 atcaggaaat ccgaaacaac tacccaagac aagcagaact tccttttgac tcagatcgaa
    16621 aattaatgtc tactggacac acaattgata acaaaaatta cttgtttgtt aaaggcggac
    16681 cagatatcgt cttccaacgc agtaaaaaag tattgctaaa tggtgaagtc gttccgatga
    16741 cggatgaatt gatgaaaaaa ttccaagagc aaaatgaagc tttttcaaca cgcgcattac
    16801 gtgtactagc ttttgcttac cgtccacttg ataattttga tttatcattt gaagatgaag
    16861 acgatttgat ccttgttggt ttgcttgcta tgatcgatcc tccacgtgct gaagtgactc
    16921 aggcggtaac agaagcaaga agtgcaggaa tcaaaacaat catgattact ggtgaccaca
    16981 aaacaacagc tgttgctatc gcaaaacaaa tcgggatttt tgaagaagga aacctggctt
    17041 tgaccgggac ggaactagat gaattgacag aagatcaact aaacgaagtt ttagaaaaag
    17101 tcactgttta tgcacgtgtt tcacctgaga ataaaattcg tatcgttcgt gcttggcaaa
    17161 ataaaggtca tatctcagct atgacaggtg atggtgtaaa tgacgcacca gcattgaaac
    17221 aagcagacat cggaattgct atgggaacgg gtacagatgt ggcaaaagat gcagcagcga
    17281 tggtattgac ggatgataac tttgcttcta ttgtcagcgc agtagaagta ggacgtaatg
    17341 tgtttgataa tatcaagaaa gctatttctt atctatttgc aggaaattta ggtgcgatca
    17401 ttgcgatcat ctttgcctta gttgctgact ggtccaatcc atttacagca ttacaactgt
    17461 tgttcatcaa tttagtcaat gattcactgc cagcaattgc attaggtaca gaaaaagctg
    17521 aacctgctac gatgaaccgc ccgccaagag atccaaattc aggaatcttt acggacaaaa
    17581 cattgatttc tgtaacgtat cgtggtgtgc tgattgggat tgctgttatc tttgctcaat
    17641 ggcttggcat gcagcattca cctgaattcg gtgtagccat gtcattttca acattgattt
    17701 ggtgtcggac attgcaaaca ttcccagcac gttcaaacac acaaacagct atccaagcag
    17761 ggttcttctc taacagaagt gtcttactag ctgtagttgt atgtagcgcg ctttactgct
    17821 taacaatgat cccaggactg aaagaagtct tctctatccc atcagcattt ggattgacag
    17881 aagtctttac atgtatggga atcgcacttg tgacaattgt tttgatggaa atcactaaat
    17941 tgatcattgt acgagtacaa ggaaataaat aaaacgaatc aacgaggttg tgagcctgcc
    18001 gtttaattct gagtcacaag gtgcaatttc aacaaacagc ttctcatgtt tgttgaatat
    18061 tgtgacttgt gaccgaagag ttggcaggag aacaccgttt aaggaagaag ttgtaaaatc
    18121 agcattttga gctaatactg agggtcatct gattggacac tgtttattcg gaaagaggga
    18181 ctttgacacg actttggtca cagtcccttt ttttatttat gatagacgga aagccaatca
    18241 ttttattatt catcaaaaga gatagaatag ataaaagagg agaatgattt attccgaaaa
    18301 gtgaaaacat ataacaaaaa attagaggaa atcaaatgaa tgaaaaagaa tgggatgaat
    18361 ttgctgaaga atattatgaa atccagcaag aatcgcaagt aactattgct gaagatgtaa
    18421 aaaattatct gaaaacggag aaagtcctgc ctgctgaaac agcagcagac gttgctggag
    18481 gatctggcag atttctttcc ttagcaaaag aaattaatcg ctatgatttg atcgattttt
    18541 cttcaggaat gctagaaaat gcaaaaaaag aagcagaaaa aaatgagttg ccaaatgttt
    18601 cctttatcaa acagtcgtta gaagaattta tccaatcaaa tgagacatat gaaatggttt
    18661 ttacagcagc aaatccagca ttggtacatc ttattcaatt agaaaaattg atgaaaaagt
    18721 caagcaaatg gtgcgtgata atcagagtag tggaatcaat cgatgatgtt ttttatccta
    18781 tcgaaaagca actaggaatg atagaagaag atcctaatac atcgccagtt gtaatggatc
    18841 aatttgaaac atacttaatt gatgaaggat tcaaagtgaa gaaaaaagat tttacttatt
    18901 ttacacagga atcaattact gaagaattga tgaagctata ctatgacgcg tacgcaaaag
    18961 atcctgttgt gaccaggtac attcgcaaac tattcagtga aaaacgagaa atccttagta
    19021 cgactaagct gacctataga ctgctccttt ggtccgcaga taaaaaaaga acctttcacg
    19081 actcgtgaag ggttcttttt atgattttat tatattttgt agtaacgaat atcattttcg
    19141 atctttgtaa tcgaaaaagg tacatctgaa gtatattctg cacattctcc gtgttcagga
    19201 agcgtctcac tgaaaatcgt ttcatattcg tcaacagtga tccgtgttcg agaatctagc
    19261 atctcttgat gagattgtgc gaagagatat tcttgatagt tttcttccag ataacctgta
    19321 aaaaattcac ttactgcacc agaaccgtag ctgaatagac cgatgcgatc tcctggctgt
    19381 agtgatttcg aattttccag tagagaagtt agccccaggt ataatgaacc agtgtaaaga
    19441 ttaccgattc gtcggctgta acggatgctt tcttcatagc gagccataag acgttcctga
    19501 ttttcttcgt ctgtttggtc taatacgctt tgcaatgcct tttttcccat cttagtatac
    19561 ggaatatgga aagcaatcgc ctgataatct tcgagtcctc gacccgacaa ttctttatgt
    19621 cgattccaaa ctttttggaa tgattcgata tacgtagaat tagataaagg accatcaaca
    19681 acaggaaatt cgctataatc tggacgccag aaatcataga tatcttctgt cagaaatacg
    19741 ctgtcgtctt caatcgataa aatacgcggg ttttgagtga tcatcatcgc aacagcaccg
    19801 acaccttgcg tcacttcacc accgcttgcc aagctgtaac gagcaatatc acttgctatg
    19861 actagtactt ttcgttctgg atgatttttg acatattctt tcgccatgtg caaagctgcg
    19921 gtgccaccgt agcaagcctc ttttacttca aaagaacgag caaaagattg gattttcagt
    19981 aaatggtgga taattaccgc actcgctttt gaatgatcaa tcccggattc tgttccgacg
    20041 atgaccatgt cgattagctc acggtctttt tctgttacga ttttgctggc agcatttgct
    20101 cctagtgtaa tgatgtcttc atttgcacga ttgactgcca tttggtcttg accaatccct
    20161 atatggtatt ttgcaggatc atccccacgg ctttctgcca actctgtcat atctaaatat
    20221 aaattaggaa taaaaaagga aagacgatca atccctattt tcatttcaac acctctttac
    20281 ttatgataaa aaacttactt tactaaggta tcataaaaag attaaaagaa atataaaaaa
    20341 gagaatattt aaggattgtt aaaatttaat cttagaaaaa aataactgga acaagagcta
    20401 tttgttttta cagcaaagaa tggtatattt ttgtaggagg tggcttactt gaaagaagtc
    20461 gttatgatag atgctgcaag aacgccgata ggaaagtatc gcggaagttt aagtccattt
    20521 actgcagttg aattaggcac acttgtgact aaaggattat tagataaaac aaaactgaaa
    20581 aaagataaaa tcgatcaagt aatatttgga aatgtcctgc aagctggtaa tggtcaaaat
    20641 gttgccagac agattgcttt gaacagcggt ctccctgtag atgtcccagc aatgacgatc
    20701 aatgaagttt gcggatctgg tatgaaagca gtgatcttag cacgccagct gatccaatta
    20761 ggtgaagcag aacttgtgat agccggcgga acagagagta tgtctcaagc acctatgctg
    20821 aaacagtatc agtcagaaac aaatgaatat ggtgaaccaa tttccagtat ggtcaacgac
    20881 ggattgactg acgcattttc aaatgcacat atgggattaa ccgcagagaa tgttgcaaca
    20941 caattttctg tgagcagaga agagcaggat cgctatgcct tgtcgtccca gttgaaagca
    21001 gcacatgctg tcgaagccgg tgtattttct gaggagatca tcccagtcaa gatttctgat
    21061 gaagacgtgt tatctgagga tgaagcagtt cgtggaaata gtacattgga aaaactgggc
    21121 acgttacgta cagtattctc agaagaagga actgtaacag caggaaatgc ttccccgttg
    21181 aatgacggtg cctctgtggt gatccttgca tccaaagaat acgcagaaaa taataatctg
    21241 ccttatttag caaccatcaa agaagtagcg gaggtcggta ttgacccaag tatcatggga
    21301 atcgctccaa tcaaagccat tcaaaaattg acagatagat caggaatgaa tttatcaaca
    21361 atcgatcttt ttgagatcaa tgaagcattt gcagcttctt ctatcgtagt tagccaagaa
    21421 ctgcagttag atgaagaaaa ggtcaatata tatggaggag caatcgcatt aggccatcca
    21481 attggtgcaa gcggtgcacg tatcttgact acattagctt acggattgct tcgtgaacag
    21541 aagagatacg gtattgcttc attatgtatt ggtggaggtt taggcttggc agtcttgtta
    21601 gaagcgaata tggagcaaac acataaggat gtgcaaaaaa aaaagtttta tcagcttacg
    21661 ccatctgaac ggcgcagtca attaatagaa aaaaatgtat tgacacaaga aactgcgttg
    21721 atttttcagg aacaaacact ttctgaagag ctctccgatc acatgatcga aaaccaagtg
    21781 agtgaagtgg aaatcccaat ggggatcgcg cagaattttc aaatcaatgg caagaaaaaa
    21841 tggattccga tggcaacaga ggaaccatct gtcattgcag ctgcaagtaa tggtgcaaaa
    21901 atctgtggaa atatctgcgc agaaacacct cagcggctga tgcgagggca aatcgttcta
    21961 tcaggaaaat cggagtatca agcagtgatc aatgcagtta atcatcgaaa agaagagctg
    22021 attctttgtg caaatgaaag ttatccttca atcgtgaaac gcgggggcgg tgttcaagat
    22081 atctctactc gggaattcat ggggagtttc catgcatact tatctattga ttttttagtg
    22141 gatgtaaaag atgcaatggg cgctaacatg atcaactcga ttcttgagag tgtggcaaac
    22201 aagttacggg agtggttccc tgaagaagag attctattca gtattttaag taattttgct
    22261 acagaatcat tggcttctgc ctgttgtgaa atcccattcg aacgcttagg gagaaacaaa
    22321 gaaatcggtg aacaaattgc caaaaaaatc caacaggcag gggaatatgc gaagcttgac
    22381 ccttaccgtg cagcaacgca caacaaaggg atcatgaatg gtatagaagc tgtagtggca
    22441 gctacaggaa atgatacacg tgcggttagc gcttcgatcc acgcctacgc ggcaagaaat
    22501 ggattatacc aaggattaac agactggcag atcaaagggg ataaactagt tggaaaacta
    22561 acggtgcctt tggctgttgc cacagtaggc ggagcttcca atatcctgcc aaaagcaaaa
    22621 gcatctttag ctatgttaga tattgattca gccaaggaac tagcacaagt aattgctgca
    22681 gtagggttag ctcaaaacct ggctgctttg cgtgcattag ttacagaagg aatccaaaaa
    22741 ggccacatgg gacttcaagc tcgttcatta gcgatctcaa ttggggctat tggtgaggaa
    22801 atcgagcaag ttgcgaaaaa attacgtgaa gcagaaaaga tgaaccagca gacagctata
    22861 caaatattgg aaaaaatccg ggaaaaataa aaaagcaata tctttagaag aataaagcaa
    22921 gatttacagt tattttattt actgcagatc ttg
//
LOCUS       DACCYH010000043        22669 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.043, whole genome shotgun sequence.
ACCESSION   DACCYH010000043 DACCYH010000000
VERSION     DACCYH010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.043"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            50..3328
                     /locus_tag="IVY85_001589"
     CDS             50..3328
                     /locus_tag="IVY85_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP8044611.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3869..5776)
                     /locus_tag="IVY85_001590"
     CDS             complement(3869..5776)
                     /locus_tag="IVY85_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP8044612.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5906..6781
                     /locus_tag="IVY85_001591"
     CDS             5906..6781
                     /locus_tag="IVY85_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP8044613.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6861..7835
                     /locus_tag="IVY85_001592"
     CDS             6861..7835
                     /locus_tag="IVY85_001592"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8044614.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8024..9160
                     /locus_tag="IVY85_001593"
     CDS             8024..9160
                     /locus_tag="IVY85_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8044615.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9297..9641
                     /locus_tag="IVY85_001594"
     CDS             9297..9641
                     /locus_tag="IVY85_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP8044616.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9912..11198
                     /gene="efmA"
                     /locus_tag="IVY85_001595"
     CDS             9912..11198
                     /gene="efmA"
                     /locus_tag="IVY85_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP8044617.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11336..11935)
                     /locus_tag="IVY85_001596"
     CDS             complement(11336..11935)
                     /locus_tag="IVY85_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP8044618.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12339..13460
                     /gene="mnmA"
                     /locus_tag="IVY85_001597"
     CDS             12339..13460
                     /gene="mnmA"
                     /locus_tag="IVY85_001597"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP8044619.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13706..15127
                     /gene="arcD"
                     /locus_tag="IVY85_001598"
     CDS             13706..15127
                     /gene="arcD"
                     /locus_tag="IVY85_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP8044620.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15141..16259
                     /locus_tag="IVY85_001599"
     CDS             15141..16259
                     /locus_tag="IVY85_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP8044621.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16319..17611)
                     /locus_tag="IVY85_001600"
     CDS             complement(16319..17611)
                     /locus_tag="IVY85_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8044622.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17761..18321
                     /locus_tag="IVY85_001601"
     CDS             17761..18321
                     /locus_tag="IVY85_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8044623.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18450..18827
                     /locus_tag="IVY85_001602"
     CDS             18450..18827
                     /locus_tag="IVY85_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP8044624.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18760..20832
                     /locus_tag="IVY85_001603"
     CDS             18760..20832
                     /locus_tag="IVY85_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8044625.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21043..21990)
                     /locus_tag="IVY85_001604"
     CDS             complement(21043..21990)
                     /locus_tag="IVY85_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8044626.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22236..>22669
                     /locus_tag="IVY85_001605"
     CDS             22236..>22669
                     /locus_tag="IVY85_001605"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP8044627.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQ"
BASE COUNT         7337 a         4142 c         4812 g         6378 t
ORIGIN      
        1 ggtaatactt taagtaaacg ctttctaatt tgttacggga ggaatacact tgttaaaaaa
       61 acgtataaaa tacagtgcgc taatgatgaa tctccttatg ctcagtaccc cggttttaat
      121 ttctgtcgtt gaagttgctg ctaacgaaca acagtctgtg aacgaagaaa atgcacacgt
      181 gaacattgaa gggatgtcaa caatggagga aataccaaga actagtgaag atatggatac
      241 ggaagaagga aatgatccta tgatgaaaga atcagaaaat gaaaagacaa aaaagcaatc
      301 tgaagaagca aatgaagagt ggaaaggggt tgttttcgga gaaagcacaa gtgctagtag
      361 gtatgccatt gaaccattag aagacggcgt gcggctctct tccacgaata acggcggaaa
      421 attccagtca agcggatcag atggaatgac ctattattat caagcaatcc ccaaagacat
      481 caactttagg atgcgggcga cgatcgaaat agaatcttgg acctatacga atgcccaaga
      541 aggatttgct ttaatggtaa gagacgcagt accaaaaaat catttgtttg ggcaggcatt
      601 ttattcaaat tcatttgcca tactaggcag tcggatcgaa tatgtctggg actcggatag
      661 acaagaagtg gtcaacacga gcggcagcaa atatacgatg cgacttggat tggggacacg
      721 ggcaatcaca ggaatatcat ctgaagatcc gcaagctgcg ccagctcctg ggagtgtctc
      781 agtggaaaca acacctttag agacttcagc agggttcttg gaaaaagaaa caggttctta
      841 taatatattc ggaaacggtc atgggaatct gtttccggcc acgaactcga ttactaaact
      901 ggacgtagag atgaaaaaaa cgaatactgg tttagaggca tattattacg atcctgaaac
      961 aggagaagaa atggcttctg acatcatgta tgactgggaa aaattatacg cttcagatga
     1021 atcggttatt tatgcaggat ttgctgcagc aaggaatatg acgatcaagg tagaaggaat
     1081 cgaagttgct tactcagatc ctgcgacaga tcctcctggg caagaaagac ctactcgctt
     1141 gattgatccg atttacactg tgacctctag taatcattca ggaaaccaag atcatactat
     1201 gtcctttaga gcaaatgctg acggattatt gacaattaaa aataaagcaa caggagaggt
     1261 gcttaagaac gcaaaaaact tggctatcac tgcgaatcgg gaatttgatt atcagacaaa
     1321 actgatggaa ggaacgaacg aatttacttt cagttttacg ccagataaaa actatccgcc
     1381 agaagaatac gtagagatga ctagttatga aacaaaagag attcttcata cagtggacta
     1441 ccgtaaacct aaatattcga cagtttatgt aacaccagaa gggtcagatg caggaaacgg
     1501 aagcagacaa aatccattat ctctgacaca agcattgaga tacgcatatg ctgggcagac
     1561 aatcgtcatg gcgcctggaa cttattcctt cgaaagagga ttgacgattc caaaaggcgt
     1621 taacgggaca agagaaaacc cgattcagtt gattgcagag aaaaatccag aaaacaagcg
     1681 tcctgtactt gattttaaag gaactggaaa tgggatgaca ctgttcagtc actactgggg
     1741 tttacgaggg tttgacatta caaatagtgc agctatgcaa aaaggattac agatttctgg
     1801 aaatcataat ttgatcgaag agattcatgc ttatcggaat ggaaatacag gaatccaaat
     1861 cagcggtagc tcaaatgatc ctttcgaagc atggcccgcg cataacttag tcaagaactg
     1921 tacatctttt gaaaatgccg atccaggatt tgaagatgcc gatggatttg cggccaaact
     1981 gacaatagga gaaggaaatg tatttgacgg ttgtatcgcg tatcataatg cagatgacgg
     2041 ttgggacctt tttgctaaaa atgaaacagg ttcgatcgga aaagtaatca tcaaaaactc
     2101 agttgcttat cgaaacggct gggtaccagg aattgaggga gaaggtaacg gtaatggatt
     2161 caaaatgggc ggttcttctt taacgggccc tcatgaattg atcaatagtt tgtctttcga
     2221 aaatcttgcg aaaggaatcg attccaatag cggaacagat attatcgtca atagcagtac
     2281 aagttttaat aacggttcat ataatgtggc tctttatacc tcttcagcag gaaacacaga
     2341 ctaccatgct tctggtatct tatctttccg aacagagaat cttgagatga gagagcagat
     2401 ccgcccattg aatcaaaacg aagatcaaat ctaccactcg ttgaactact attggaatga
     2461 acgagagaaa caatcagaaa atactcttgg acataccata tcaaaagatt ggatcgagtc
     2521 tctaaataca tgttcgaaag aaggtcagca gcctgttacg cgctatgaaa atggaagtat
     2581 ccatgttcat ggactgatgc agattaaacc ggggaatcgt caaacagaaa aggaaagagt
     2641 ggatggtctt ccattatctg taggtgctat ttttgacgga gaaacctcac cgtcttcgga
     2701 atatccagag tatgcaatcg tacaagaacc aaatcagaca aaaccaaatg aacaaaataa
     2761 tccgattatt atacaagaag caaatggtgt ggcggagaat gcagaaggaa atatcattga
     2821 actgaagcct tttacagttt ttgtgtcaac tttcacacca aatcaaggaa cgcctgtctt
     2881 tgaagatatg cgttatttcc cagaaaagat ccaaatcgtt gaaacacagt caagtgctca
     2941 attttctaat cataccgcta ttttttcagc accttcaaaa ggagaatata cgctagaagt
     3001 cacttatcgt tcggaaatat ttgatgggga aaaatggtct gctcatacag aatctgttac
     3061 ccagcaaaaa gcgatcactg tgcgagaaac tgccgatgtg cccccgggaa atgacaaacc
     3121 agaaagagaa aatccaggaa atgaatcacc aaatggaaaa ccaaatggaa aaccttctca
     3181 tccggatagg acagcaaaag aaagtgaaaa aaatggcact caaggactcc caaaaacggg
     3241 agaatttaat aacccgttgc tagcattagc tggaggaatt ttgttaatcg gtgtagttgt
     3301 ttatgtcatg aaacagcgaa aaaaataagt ggaatttctc cttttatgat gtaaaacaat
     3361 tgactgagga aagtaaaaat agaaggaaat gagaccaata gatgtctcac ttccttctat
     3421 ctttttatag atgaggttct ccttgctgct tttgattggc tgttcttatt acttgatgca
     3481 ggacagcttt ttcacaatct ctccactaaa cggtgttcaa tcagctgacc ctcggtatta
     3541 gctcaacaaa cggcaaaata tgaggaacta tttttccgtt tttttctcta atactcaggt
     3601 caggacgctg atttcacaac ctctcgataa acggtgttca aaagccagtt tctcggtagt
     3661 aagtcaaatt tatgcaaaaa tgtggaaagc cattttcaca aaatttttct tactacttga
     3721 aactaagcag ctttttcaca acctctaata catggtattc cagaagtaac ttcttcgaaa
     3781 aataagccga aatgtcacaa aaatttgaga agcgattttc ccaaattcct tcttatttct
     3841 tgaagctgac cacttctggc acaacctctt accagattcg cactcttttt tctggcgcta
     3901 gatacatagc gtcttgttcc gtgataccaa atgtttcata gaattcgtca aggttttttg
     3961 gttgcacgtt tgctcggagt ttagcagggg catggacatc gatttgcagt aaaagttgtt
     4021 ggtattcttt ttttgctttt gtacgccaaa tcgttgccca attgacaaag aattcctcga
     4081 tagaaagatc tgattccgtt tttgctgctt ccaatgcaca gcttagaccg cctgcatcag
     4141 caatattttc cgaaacggtt aattttccgt ttacttttcc gtcagcaaaa ggcagaccat
     4201 cgaattcttc gatcattgct tgtgctttgt tttgaaattg tgtaagatct tcttctgtcc
     4261 accagttgtt caagttgcca tattcgtcaa ataaagcgcc gttattatca aatgcatggg
     4321 aaatctcatg agcgataaca gctccgattc caccataatt agcagagctt gattgttcta
     4381 gactgtaaaa tggtgcttga aggatcgctg ccggaaagac gatcacattc cgaaatggat
     4441 gataataagc attgaccgtg tcagcactca tttcccattc tgaacgatcc actggtttgt
     4501 tccatttgct gaaacgatct tttaatgtga gtttgctgaa aacaagagta tttgaaagca
     4561 aagtaccacc ctgatctgca ggtactgttt tgaactggtc gaataatgct gggatcttat
     4621 cggggtagcc tacttggata cctaagttat ttaattttat gacggctttt tcttttgttg
     4681 catccccaag ccagtcattt tcttctagac gttttttata aacggcgatc atttttttca
     4741 ccatttgttc tacgtctttt ttagcagctt cgccaaaata tttttttccg tagtaatcgc
     4801 caactacttg atcgaattgt ccagaggcaa ggtaatacgc tgcttttttc tgaggcattg
     4861 cttcgtctgt tccagacaat acgcgagaat agattccgct gacttggcgg aattcttctg
     4921 atagataacc actaagagaa cgtactgttt taatgatcat ccagcttttg aataacggaa
     4981 aatgagcttc tgtcaggatg tcattcaaat gatcgaagta cactggatca gttacgatca
     5041 ctttatctgg agttgtccca atcaaacccg tgacaagttt tttcaagtcc agtttatctg
     5101 tgtatttaat aaatgtatca aaagattgcg gattatacat tttgctgtaa tctgcgtttt
     5161 cttctgcact ttttacatga ggagcaatca atttatcaaa ttgcactgct tgttccgtga
     5221 ttgcttctgc ctgacttttt tctttgccgg caagctctaa taactgaaca gccatatcaa
     5281 agaaaacgcc tattagttgt gcaccgttgg gatggtctga tgcataatac gtcttatctg
     5341 gaaggaataa agaaggcgga taagcaaaga aggcgttcgt ttgcgcattc ttcatatctg
     5401 catctacatc cagattaaat ggcaatggta atgtatccaa cagccattct ggaaattgct
     5461 tattcaattc gtcgaaagac tgcaatgatt caactttttc gattataggt tgaagcggtt
     5521 gagcatgcaa ctcatttctt cggtcataat cgtttgccag ttgataatat gcaaggaaat
     5581 gtttcatttt ttctgaagac actttttctg gatgggcaag cagatcgtct gtttctccca
     5641 tcaaaagttg gtcgatcccg tcaactaaat cttggaaacc gcctgtagct ggtttatctg
     5701 cagggatttt tgctgttttt tgccattctt cattgactgc ttcatagaaa tcttgtttca
     5761 atcgttcttt gttcattcat cgatcgctcc ttttttgttt cgtgtgccca ttatcttaca
     5821 aaatgaagga attaacaaat agaacataaa aaattgaaaa ttcgaacaat caaactgatt
     5881 tgtttttttc aatccttcac ttataatgaa aaaaaggagg cgatcccaac tgaatcaagt
     5941 agtggataaa cctttatatt tcaaagtaga taaaaaaatt aaaaaattag ccagcactca
     6001 gcagctgcag tcaagaaaaa gtaaattgct ttttttagct ctggtttttg aagatcaaag
     6061 ttatgtgatt atcgatcaat caggacatcc ggtagaatat tctccagcaa aatatactta
     6121 tcaagagggt ctttcttgta aaaaatggaa gctgatcaat gaagagccaa tcgagttatc
     6181 tagatggatc aacagaaaag aagatattcc cgttttgatc gaagaaaaaa ggtcaggaaa
     6241 ggaactcgca aattgctggg tgggattgcc agaagaacgt tttttgaggt ataagaaatg
     6301 ggctaccccc tcaggctatc tttgtggtac ttacgcggca gcagttctcc ttgcctatta
     6361 tcaagactat cgtaaggggt ggatgcttcc aaatgagatt cgcaagaaaa atacggctga
     6421 ttcgttggtt ttgacgaagg cattgcgtag ccagatccaa ccactaggac ttcctacgat
     6481 tccttttcag gttagtacag ggatttctag ttttttaaag aagaatggca atcatgagcg
     6541 tgcacgagca actcttctag gttcatggca gcgagctaca aaacgtatac gtgaaggcaa
     6601 acctgtgatg attggtattt tgaaagtact tggcagtaca tatggcaatc attgggtgac
     6661 agcgtacgct tattttgaaa ctgaaacagg agaacgttat tacaaagtac acgacaactg
     6721 gggcgattat cataaagtca ttcctgccag ttggagtaac ggaacagttt cacttcctta
     6781 agaatttgct gaaattaaca gaaaatcttg gaaaaatatg ttaaactatc gaagaataga
     6841 gaagttttgg ggaggactac atgagtaata agtatcagga tgacacgttg aagattttta
     6901 gtttgaatgg caaccgtcca ttagctgaaa aaatcgcgaa agtgttcgga acagaattag
     6961 gtaaaagcgt cgtgaagcaa tttagtgatg gcgagatttc catcaatatc gaagaaagta
     7021 ttcgagggga tcatgtgtat atcgttcaat caacgaatgc acctgtcaat gattactata
     7081 tggaactact gatcatgatc gatgcaatga agcgagcaag tgcgaaaacg atcaatgtcg
     7141 tattgcctta ctatgggtat gctaggcaag accgcacggc gaaacctcat gaaccgatca
     7201 ctgcgaaatt gatcgctaac ttgatcgaag aagcaggggc aactcgagta ctgactttag
     7261 atctgcacac ggttcaagta caaggattct ttgatattcc tgtagataat ttatttacga
     7321 tgccgttgtt tgctcattat taccgtcaat taggtctgac gggagatgac atcgtagttg
     7381 tttcgccaaa aaacagcggt gttcagcgtg cacgtagtct ttcagaatat ttgaacagca
     7441 cattagccat cgtagatcat gcagatgatg cttcaactga gggtggttat gtgatcggtg
     7501 atgttcaagg aaagacatgt atcatggtag atgatatctt gaacacagga gcaacgcttg
     7561 cacgagcagc aaatgtatta aaagaaaacg gcgcaaaaga agtatatgcc tgtgcgtcac
     7621 atggattatt gtccgatcat gcgaaaagta ttttagacaa tgcaccaatc aaagatattt
     7681 gtatcactga ttctgttttc acagaagaaa atcgtcatcc agaaaactta acaatcgtta
     7741 catgttcaga attaatgggt gaagcggtca aacgcatcca tgaaaataca ccgatgagtc
     7801 cgttattccg tctggaagaa aaagaattcg aataaacaaa aaaaagaggg aaagaagttg
     7861 tgacagaagt gagcagcttc aaggagctgc gacaagactt ttgtcacagc tccttttttc
     7921 tatgcccatg aaacgtcctc tagacaaaaa tgagctcttc tttggtttgt tcgctatttt
     7981 atgctagaat gtgcctagca ataatttgga agggatgaat gaagtgaaaa ctatttatct
     8041 ggatcatgcg gcaactaccc ccatgcatcc acaagtaatt gaagcgatga cggcaatcat
     8101 gcaagacacg tttggaaatc cttcaagcat acatggattt ggtcgccacg cgcatgaaaa
     8161 attggaaaca gcaagacaaa tcattgcaga cagtctgcaa gcaaaacctc acgagatcat
     8221 ctttaacagt gggggaacag aaggggataa tacagctatc ttagaaacag cgttctcgcg
     8281 tcaaaaagaa ggcagacatc taatcactac agctatcgaa catcctgctg ttcttaatac
     8341 catgaaatat ttagagacaa aggggtttga cgttacatat ctgcctgtag atgaaaaagg
     8401 gaatctgtcg atcgaagagg tcaaaaaagc gttacgagaa gatacgatct tggtctctgt
     8461 gatgtacggg aacaatgaaa tcggaaattt gatgccaatc aaagaaatcg gtgaactttt
     8521 agcggatcat ccagcttatt tccatacaga tgcggtacaa gcgtacggaa accaaacgat
     8581 ccatccagaa gaattaggaa tcgatttatt aagtatctct gctcataaaa tcaatgggcc
     8641 aaaaggcgtc ggtttcttat ataaacgaga cggagtaaat atccctgcat tgcttttagg
     8701 cggggagcag gaagaaaagc ggcgtgcagg tacagaaaat ttagcaggga tctggggtat
     8761 ggcaaaagca gttgaactgc tgtcacctga agaacgtgaa gcacgtaaca acaaatataa
     8821 aggattccag caattgattc ttgatacatt agaagcaaac gaaatccctt atcaagtaaa
     8881 tggtgattta acgaataaac tccctcatgt gttgaatctt cgcttaattg gcgaagcaaa
     8941 tgatttgcta ctgatgaagc ttgatcttaa tggggtcgct atttctactg gttcagcttg
     9001 tacagcagga aatatcgagc cttcacacgt attagaagca atgtatggag aagatacacc
     9061 gatactaaaa gaatccatcc gtatcagctt tggtttggga aataccgaag acgacatcca
     9121 gtatttcctt gatcaattag ttcgtgctgt caaaaaataa ctagtgaagt cattcttata
     9181 tgaacagatg tctgagagaa cgccttcatt ttttagggaa cttaactgaa aaacctagta
     9241 aaaatcagga taaactttta taataaagag aaactaattt tatcgaggtg aaaatcatgg
     9301 catttgaaac aacagcaacg gtactgggcg caccaattag ctatcgtttg catcctgatg
     9361 caaaaagata tacattgcgc gataatggat ttacagaaac aaacggaggc aattatcaat
     9421 tgatccgacc attagatgcc acgccgcaaa gcaaagaagg attcaaatta aaaatcactg
     9481 tatcaaaaga tattcaaaca ttgaaaatgt cgatcactac agcaaatgga ttgaaagcag
     9541 tcaatatttt caaagatcca aaacagaaaa tgagccaaga taaattttat tttctgatgg
     9601 atgggatgat tagccgtggg ttattcgaaa aagtcgaatg atctgtggat ataaaaagac
     9661 aactagtttt gaaacggcct attgttagat gactaacttt gtaaggacga cgcaaattag
     9721 ttgtcttttt tatttttaaa tttaacacat gttgttattt ttaatatttt gctgatttat
     9781 tgtttctaag taaagaaatt taaattgaaa cgattgttat ttagggaaaa aggtattttt
     9841 tgaaaaaaga atcccgtata atgattgttg tgcaaacgct atacaaaatg aaaaagaaaa
     9901 gagggatgtt tatggaaaat gaacagtcgg tcgttttaac gaattggaag agaaattatt
     9961 tgttcttttt atcgggacag tttttgtcag gaatcactag tatggtcgtc caatatgcga
    10021 tcatctggta tttgacaaga gaaaccggtt cggcgacaat tttgagtttt gctactttac
    10081 taggaatgat tcccatggtt ttactaagcc catttgtagg accgctagtg gatcgctggg
    10141 ataaaaaagc tcttttgatt gtcacggata ttattgtagc gattttcgcg cttattttag
    10201 cagtagtcgg aacgatttca gaatccttcc cgatttggct agtttttgtg tctttgttta
    10261 tgcgttcggt cgcacaaaca ttccaaatgc caacgatcca gtcgattatg ccgacaatcg
    10321 ttccatcttc tcacataaca aggacgaatg gacagttagg aatggtccaa tcagcgaatt
    10381 tcatcattgc gccagctcta ggtgcggctt tgttttcagt cgttcctgtc aattacctta
    10441 ttttattaga tgtattgggg gcagtttttg gtgtcggctt attgattttt gtgaagatcc
    10501 caaaagtttc tcctgaaata ttggaagttc cgcttactat ttttaaagat gcaaaatttg
    10561 ggctgcagca attgatggat aataaagggt tatggtacat aacgatcaat ggtgcatttg
    10621 tgatgctgtt gttcatgcca gcaatcagtc tgtatccatt gatgacgcta gattactttg
    10681 gtggttccgt tggacaggcg ggagcagtgg aagtagtcta cgcagtcgga atgctcttag
    10741 gcggtgcact gatcagtttt ataggaacat ggaaagaccg aatgaaacct attattatag
    10801 cctacatcat tatgggcctg acgatcggtg caagcggatt ggttccaaac gatagtcaag
    10861 ggttcttgta ctttcttatc ttgaatgcag gagcaggttg tgcaacgcca tatttcaata
    10921 cactgctgat ggcaatgatc cagcagagtt atgaatcgaa tgttttaggt cgtgtcttag
    10981 ggaacttcaa ctctttgatg aacctggcag gtccaattgg attattattt gcaggaccat
    11041 tagctgatcg tttaggggta gaaaaaatgt tcttgttttc cggaatcggg attttattat
    11101 gcggaatcgt tttattttta acgtcagctg cccgaaatta tgataaagaa ttacagaaaa
    11161 aactggtaaa agaacaccat gagcaaaaag acgaatagta tggaatggag ttgtgacaga
    11221 agtcttgtta caactccatt tctgatccta atgttcctgt ggcaataagc ctactcaaat
    11281 gaaaaatatg agtaattatc tttatttgtt taatacttga tatagggcag ttttttcacg
    11341 acctatcgta cttcctcttt gcttcataga tttctttgat ttgttgctca gataacggag
    11401 tgccaaatgg tagtttttcc tttaagagtt ctctttccgg tgcgtctacg cctacattga
    11461 catcgttctc gataggtacg ctgtaatgat ggatatgtcc atgaagattg cggatagtgt
    11521 cgttaggccc gagcaacatc ggataatgag tcaagtaata ttgttgatgg tcgaatttaa
    11581 cgatggcacc tacatcataa aaataaaatt tgggacttcc gtcagtaagc ctaggatcat
    11641 ttttttctaa ataacggaaa aaagcgcgac tgtcatggtt ccctttgatc aagtggattg
    11701 ttccttgcag ttgagtgacc agttccaaaa tttctgcatt tgctttttca tattgcggat
    11761 gcatagcaag gtcgcctaga tgataaacat ggtcggtttc ttttattact gcattccagc
    11821 tagtgatcaa ctgttggttc atttcatcca catttgcaaa gagccgcgga gcaaagtcgt
    11881 tttttcctaa taagcgttca tggaaaaagt gcatatcact gataaagtaa ttcatgtatc
    11941 ttttgcctcc cttttttctt agagtaaaca aaatcactta tctgagcaac tcataacatc
    12001 taaaaagaaa aggttgtaaa tgagacgtgc agctttaagc gataagaagc aattttgaaa
    12061 aatagttttt cacaatttat aaaattattc cttattgccg aaaggctggc cctttttatg
    12121 agtatgccaa ttttttatat cgaatgctca aaagatgagt gattttttgg ttgactatca
    12181 tgtttgtgta ctgaataggt ccagtagaac agtattttta cgacttgata attaagggat
    12241 gaaattttca gaaatatgac gtataatgta aaatactgaa aagttgcgac cttcaactcg
    12301 tagatgtttc agaatctaat tgaaatgatg gtgatatcat gacagacaac agcaaaacac
    12361 gcgttgtcgt agggatgagc ggcggtgtcg actcatctgt aacagcactt ttgttaaagg
    12421 aacaaggata cgacgtgatc ggaatattca tgaaaaattg ggatgacacg gacgaaaacg
    12481 gtgtatgcac ggccacggaa gattataagg atgtagcgaa agtagctgct caaatcggga
    12541 ttccttatta ctctgtgaat tttgaaaaag aatattggga tcgtgtattt gaatatttcc
    12601 tagccgaata ccgcgcagga cgtacaccta acccagacgt gatgtgcaac aaagaaatca
    12661 aattcaaagc attcttggat tatgcaatgg atcttggcgc cgattatgta gctactggac
    12721 attatgcgca agtcactcgc gatgaaaacg gcgtggtcca catgcttcgt ggagtagaca
    12781 ataataaaga tcaaacttac ttccttagcc aattatcaca agaacagctt tcaaaaacga
    12841 tgttccctct aggcggcatg gaaaaatcag aagtacgggc aattgctgaa cgcgcaggcc
    12901 ttgcgactgc taagaaaaaa gattcaacgg gaatctgctt tattggtgaa aagaacttca
    12961 aacaattttt gagcaactat ctgccagcaa aaaaaggaaa tatggtcact cttgatggtg
    13021 aagtcaaagg acaacatgct ggcttgatgt attatacgat tggtcaacgt caaggactag
    13081 ggatcggtgg cggaggagat tcgcaagaac cctggtttgt tgtaggaaaa gatctagcga
    13141 caaacacatt atatgtgggg caagggttcc atcatcctgc attgtatgcc acaagcttgg
    13201 atgcaagtga aatccacttt acgacgaatg aaccaatgcc aaaagaattc aagtgtacag
    13261 cgaaattccg ttatcgtcag caagacgttc ctgtgacagt tcgcttattg gatgataatc
    13321 gtgcagaagt cgtattcgac gaacctgtac gtgcaatcac tcctggacaa gcagttgtat
    13381 tttatgatgg aatggaatgt ctgggtggtg gtttgatcga ccacgcatat caagaaacaa
    13441 aagttttaca atacgtttaa gcgatattaa atgaatcaaa acagctatgg ctaacaaaag
    13501 aaacaccttt tcttttggat agcatagctg tttttttgca ataaaattaa tttttattca
    13561 ttttatactt gaaatacttg tgtaaaaagt tcataatatc caataatatg aataaagttg
    13621 ctttttgtta agcaagagca gttgcaaaat agaagggaat cttttgcaag tgatttgttc
    13681 aattttataa gtgagggtga tggaaatgga aaagaaatcg acgggtatta gcaaatgggg
    13741 actggttgct ctggttgtca gttcgtcaat cggaagcggt gtgtttggga tcacaagtga
    13801 tctggctagt tctgcagctc cagggccagc gatcctttca tggataatcg ttgggattgg
    13861 tattttagct ttggtattat cgctgaatca tttaggagaa aagcgtccag atctagatgg
    13921 tggtattttc ggttacgcgg aagcttcatt tggtaaatta ggtggcttca tcagtggttg
    13981 gggttactgg ttgtctgcgt ggctgggaaa tgttgctttt gcaacgatgc tgatgagcgc
    14041 catgggagaa tttttcccga tattcaaagg tggacaaaat gttccttcta ttttatttgc
    14101 aagtattttt atttggggtc tgactttatt agtaaacaat ggagttgaag gagcgagcgt
    14161 catcaataca atcgtgacga tctgtaaatt agtgccatta ttcctatttt taatatttgt
    14221 atttatcgca tttaaagtga atatattcac tgctgatttt tggggtaacg tatctgataa
    14281 tctagtgaat ggaacagggc agcaaggaac gatctggttg cagattaaag gttgtttgat
    14341 ggttatgatg tgggtctttg tagggatcga aggcgcaagt gttttagcca accgcgcgaa
    14401 gaaacgttca gaagcccagc aagcatcgat cattggtctg ctttgtttat tgtttatcta
    14461 tatccttgct tcgatcttgc catacggcgt cttatctcaa gaagagttag caactatttc
    14521 acaacctgct atggcaaata ttttaaaagg aatcgtggga aattggggag cagcgctgat
    14581 caatatcggc ttgattattt cgatcattgg ttcttggttg tcttggacga tgctcccagc
    14641 agaaacaacg atgttgatgg ctcgagacgg tctgttgccg aaaggttggg gaaaaacgaa
    14701 taaaaaaaat gccccaactt attcgttagt tgttactgca atcttgacta atctattctt
    14761 attgactttc ttagttactg attatgccta tcaatttgcc tattctttat gtacagcggc
    14821 aattttgatt tgttatctac ttgtcgggat ctatcaagtc cagttttctt taaaagaagg
    14881 aatcaaaaaa caagtagtga tcggtgggat tgctgtagtt tttgaattga tgggcatcat
    14941 tttagcagga ttttcttatg tccttttatg tagtattgcc tatcttcctg gtttttgctt
    15001 ctactggatc gcttgtcgtg aaaccaaaca tcaaattacg ggaaaagaaa aagtaatgat
    15061 tgggctgatt tgtgtggccg cactgctttc aatcgggttg cttgctgtag gctggatcaa
    15121 gatataaggg ggaaagaaac atgaaaatcg caaatttcgg tgtagaagaa tggctaaata
    15181 aatgggaaaa gaaagcggta tatgatattg cacaaagctc gatcgaagca ttgacattag
    15241 acgaactcgt tggcttagat gggactagcg taagtaaatt tttcgaaaaa cgcaaaaacg
    15301 aaccattgga ctatggctgg atcgaaggat cagatgtttt caaacaagaa gtcgcttctc
    15361 tttatcaaac cgtcgatccg gaaaacatct tacaaacgaa tggagcgact ggtgctaatc
    15421 ttttagctct atacgcgtta gtggaagcag gagaccatgt tgtttcgatg cttcctactt
    15481 atcagcaatt atatgacatc cccaaatctt taggtgccgc tgttgacttt gttcatttga
    15541 aggaagaaga agattggcag tttgatttag aacagctaga agccttagtc aaacctgaaa
    15601 caaaaatcat ctgtttgaat agtgcaaaca atcctacagg cacattgcta gatcggaaaa
    15661 ctttagaaaa aatcgcagaa attgctcgta cagtagatgc atatgtattg atagatgaag
    15721 tatatgcgcc tttaacggat aaaggtgaat tcttgtcgat cgtagatgtc tatgaaaaag
    15781 gtatagcaac caattcccta tccaaaacct actccattcc aggtattcgt attggctgga
    15841 cagctactgg acctgagttg gcagaagttt ttcgaaagta tcgagactac acgatgattt
    15901 gcggcggagt cctgtcagat gatcttgctg tacatgcatt gaagaataaa gaaaaaatat
    15961 tggcgcgaaa caaaaagatc attacagaga accttgtgat tttaaaacaa tgggtagcca
    16021 atgaaccgaa agttgaactt gtcgctccca attacgtctc tacttctttc attaaattag
    16081 ccatcgaaga agatgaccag acattttgta tcaatttatt agaagaaaca ggagtactat
    16141 tagtaccagg aagtgccttt gatttaccga agcatgcccg actaggttac tgttgtaaaa
    16201 aagaaacctt ggaaaaggga ttattcctct tatctgagtt cttgaaaaaa caggcctaaa
    16261 caaaaacact gcaaggacag tctgtgacaa ctgttcaagc agtgtttttc atttacgctt
    16321 attgtctatg agaaggagag tgccctgaag ctatggagac tcctgaagct ttttcttctg
    16381 cagtttcttg ctgatggatc tcttgtgtat tgtgtcgtac aagcaagaag ttgaccaaaa
    16441 caagaagaga agtcgaaaga aagacgccgc gataaccaaa tgcactggaa acactggagc
    16501 cgatcatagg ccctactacg ttaccagttg cttggaaaga ttggttataa ctgaagatcc
    16561 gtccagcagc ttgatgaggc gaatattttg tgatcaatgc ttggacagca gggagcagac
    16621 aagcatcaga gataccaatc aaaaaacgta aggcagctaa ttgccaaaca ttcgtgacga
    16681 atgccattgg gatataaacc agaatcgcaa aacccaaacc aatcgcaagt attctttcac
    16741 tcccaatctt gtcacctaag cgaccaaatc ttggagcggc tatcaaagtg gcaatcccag
    16801 gtatcgaagc aatgattccg ctgatcaacg taacattccc atgtccatgc agcaattggc
    16861 gaatgtacag actgatgatt ggactaatgg aattgttgga tgcttggatg atcatcgttg
    16921 tgacgaacat accgatgacg acgtgtggat atttcaagtc tttgaaaatc tgtttggcag
    16981 aaaccatgtt ttcttttttc actgggacaa atttttcatg gacgaaaaac agactcaaca
    17041 agaagacgaa aaataagatg atccctgtaa tgaaaaaggt agggcgatag ccaaacgctg
    17101 aagcagagat cccgccaaga agcggtccaa gtaatgttcc tgtaacagaa ccagttgcta
    17161 aggttccaag aacttgacca cttttttctt ttggcgtccc agttgcgaca agggcagtgg
    17221 cattactgat ataaccagaa aaaatccctt gaagcagacg caatgcgact aattgataca
    17281 cactagtcac gcaacccatc aaagaaatga cgattgccat tcctaatgaa gcacgcaaca
    17341 gcatcaattt tcttcctttt tggtcggcta attttcccca ccaaggagag ataagcgtag
    17401 tcaccaagaa agtagatgaa aaagtcaatc cgctccaaaa gttcaattgt gacgtactat
    17461 aattacctaa cgtatctatg taaagtgaca taaaaggcat gactaaacta aatccgatac
    17521 cagccataaa tgttccaaac cataagacaa acaagttttg ttcccaagtt tctcttttcc
    17581 taaataattt ttcctttatt cttgcagcca attagactcc ttctttctga aaattttcat
    17641 ctacagctct ttattataaa ctacaatatg tagtttatca atgaaagtct cttttttctc
    17701 tttctttttc gtggtaaact gaaagagtga aggatttatt ttatgagagg aaacatggaa
    17761 atgaaaaaaa gaaatctgtc tcaagagaaa atcatcgact gctttcgaga actagcagaa
    17821 gagatggatg ttcagcaaat aacctttcag catttagcca aacgcttgga tatcaaggct
    17881 ccctcgctgt ataaccattt caaaaatatt cgtgatgtca aaacagcgct aacagcgaaa
    17941 cttctgcagg aacttaatga ccagctgcga cgggctcttg tgggaaaaag tggtcaggaa
    18001 gcactgcaga tctatgcaga aacttatcga tcctttgctt tttcaaatca agcagtgtat
    18061 gagttactga tcagtgtacc gcataccaat gaagctgttt tattagatgg aatccatgaa
    18121 acgaatcaga tcattttgca gatatttgag gcttttcaat taacgaaaga agaaaaagtt
    18181 catcgaagca gggaacttcg tagtatgatc cacggttatc tttccttacg ttttttagga
    18241 tactttacaa aagaaccaac aatcgatcca gaagacagct atttttggat gatcaataac
    18301 tttatcgcta ctttgcctta aacaatcaga ttcatttgta acaattttaa gatgttcatt
    18361 aaatgaccct tttataagag aaaattcttg caactttctt gaaaagtgac taagatgagt
    18421 ctagaaaagc tgaaagaagg agcacgagta tgtatcaagt aattaccatg ttcggggaca
    18481 atgagccttg gtggtttttt gaagactggg aagaagatat tgaagaagaa gaaacattcg
    18541 agtctttcga agaagcacga caagcgtacg aaaaacaatg gaacgaaatc catcaaaatt
    18601 atgaatatat caatgcaaaa agtaactttc tcagcgcctt ttggaatgac ggagatgagc
    18661 gctggtgtga agagtgtgac gatgatttac agcaatacaa gggacttgcg ttgttgaaaa
    18721 atcatcagcc catcacagta gaaagcagaa aggaatttta tgaaacaact aattctagcg
    18781 gaaaaaccaa gcgttgcgaa agacctaagc aaggtgcttg gtgctaatca aaagcacaaa
    18841 aattattatg aaggacccaa ggtgatcgtt acttgggccc ttggtcattt attaggattg
    18901 aaaatgccgg aagatctgaa taaggaatgg cagagctggc aaatggacac gttgccaatg
    18961 atccctaaaa aactaggaat caaaccactt cctaaaacag gacatcaatt aaaagcaatc
    19021 aagcagctgg ctcagcgaaa agatgtctca gaagcagtca ttgcgacaga tgccggaaga
    19081 gaaggggaac ttgttgctcg ctggatctta gagtatgtgc atttcaataa accagtgaag
    19141 cggttatgga tctcttctca aacagataaa gcaatcaaaa ctggattcaa gcaactaaga
    19201 cctgcgaaag aatatgatgc attgtatgaa tctgctcttg cccgggcaaa agcggactgg
    19261 ctggtaggat tgaatgtgac cagagcctta actgtgaagt acgcggataa tttatcagca
    19321 ggccgtgtgc agacaccgac actagcatta gtgagagacc aagaaaagaa aatcgagcag
    19381 ttccgtccac aaacgtattt cacgatcacg ttgcaagctg gaaaagaaca agccaaaatg
    19441 cttcaaaaaa atcaatttgc tttaaaaaca caagaagaag cacaaaaact tgttcaattg
    19501 atcagtacaa gcaaaggaac tgtcacagaa gtccaagaaa aattaaaaac ggaatctgcg
    19561 ccattgcctt atgatttaac agagatccaa agagaagcca atcaacgcta tggctattct
    19621 gcaaagaaaa cattaagtct tgtacaaagt ttgtatgaga cccataagat cgttacttat
    19681 ccgcgtacag acagcaaata tctgacaaat gacatgaaaa gtacgatgaa agaacgattg
    19741 caagcagtca gcgactttgc tcctgaggtc aagcagtatt tgaaaaacgg agccatcgtc
    19801 caacagacaa aagtattcca agacagtaaa gtgaccgatc accatgcact tttgccaact
    19861 gaaaacagag cacgttatga aaaattaagt aatgaagagc gaaaaatcta tcaaatgatt
    19921 gtctctcgtt ttttaggact gtttgctcaa ccccataaag tcagccaaac aaaagttacg
    19981 gttgaatttg gtaaagaacg attcattttt cggcaaaatc gtgtcgtcca agcaggatgg
    20041 aaaggcgaaa ctgaaactga gacggaaaca gtcaaatggg aaaaaggaat gagaatcaat
    20101 cccgatttcg ccatcaaaaa agaactatct gcaccgccta aaccattgtc agaagcaagc
    20161 ttgcttggtc aaatggaaaa atacagtctg ggaaccccag ctacgcgtgc agagattatc
    20221 gagaaactaa tcaaatctga actgatggaa cgtacccctc aaggactgca agtatctcct
    20281 aaaggtaagc aattattagt attggtaaat ccctctttag taacaccaga tttgacagaa
    20341 agctgggaaa aagaccttga agctattgct gcaggaaaga aacaagcagg aaccttcttg
    20401 aatggaatcg aaaaagaaac aaaacgcttg gtgaatgaga tcaaatcaag caaacaagaa
    20461 tatcaagatt tctcgattac ccaaaagaaa tgcccagaat gtggggccaa tctgcgcgag
    20521 aaaaacacac gtgacggcaa aatctatgtc tgcaccaatt cagattgcaa ctaccgcaga
    20581 agaaaagatc caaaagtctc taatcatcga tgcccgcaat gtcaccggaa aatggaaatc
    20641 atcgaaggta aaaacggtgc ttatttccgt tgtaaatacg atggaacgac agaaaaattg
    20701 cttgataaaa aagagcagaa gaagaaaatg accaaacatg aagaacgacg cttgctgaaa
    20761 aaatatgcac aagcagaaga accagaagaa agcccattag cagcagcact aaaagcagca
    20821 atgaagaagt aataagatat gaaagaggtt gtgatagaag tggtcagctt caagaaataa
    20881 gaaggaattt acgaaaattg cttctcaaat ttttgtgaaa tttcggctta tttccgaaga
    20941 agctacttct ggaacaccat ttattcggaa aaaaggactg tgacaagact tttgtcacag
    21001 tcctttttca tttaaaaaaa tgtttacttc atttgacgta tattattggt gaggcttcaa
    21061 ttcatttggc ttcacttttt ctgtttctct tatgatataa acaggacgct gtttcgtttc
    21121 taagaagatt ttcccaatat atttcccgat gattccaaga cataacaatt ggattccacc
    21181 aatgaacagg aagatcgaga ccatggaagg ccatcctgat gtaggatcac caaacaacaa
    21241 tgcccgcaaa ataatgaaca aaattgcaat accagaaccg acacaagaca aagcgccgat
    21301 aaatgaagca atcgttaacg gtgcatccga gaagttcacg ataccatcaa tcgagtagtt
    21361 gaataatttc cagaaggacc aagatgtttc gccagccaca cgttcgcggt tttcataaga
    21421 aatatattct gttttaaatc cgacccagct aaataatcct tttgaaaatc ggttgtattc
    21481 tgttaattct aggacggcat ccaccatttg acgagtcatc aagcggaaat cacgagcgcc
    21541 atcgaccatt tctgtatcgc tgattttatt gatcaactgg tagaattttt tcgcaaagaa
    21601 actgcgtatc gcaggctcgc cttcacgagt cgaacgccgg gtgccgacac aatcgatatc
    21661 agaagtagtg atccggttga tcatctcagg aagcagttcg ggcggatctt gcagatcaac
    21721 atccatcacg gtcactaaat ctcctgtaga agcttgcaat ccagcgtaca gagccgcttc
    21781 tttcccaaaa tttctggaaa atgagacaaa gcggacataa ggataagctg aggctagctt
    21841 cttgattgct gctaacgtac cgtcttttga tccgtcgtca ataaacaaat attcgaactc
    21901 atgggcacct ttgaattttt caacctcatc aaaaaaaaga ggaacagttt tttcttcatt
    21961 aaaacatggg acaacaattg atactttcat cgtttctatc ctttcaatcg tatgtttata
    22021 tatggtttac attcaaaatt taataaacct taaataacac ctttaaaatt tgattttacg
    22081 taaaagaaca ggtattcttt ttgtaaacta acgtatttag tttaccatgt ttcctcggtt
    22141 tatcaaactt ttaagaacaa tttttattag ttggcaagta gttttggata gagaaaaagg
    22201 aggaagttcc agtctattga ctaggagcaa acaaaatgaa aatacacaca cctatcggct
    22261 ttttatggtt agatgcagat gaatccggat tgactgctgt atcttttcag ccaatttcag
    22321 aaagcgaatc agcgaatcag cgaattttaa cgcgcacaca agaacagttt gaagaatatt
    22381 ttgcgggaaa aagaaaagaa tttgatgttc ctttatctat aaagagaggt acgccttttc
    22441 agcaaaatgt ctggaaagct ttgtcgacca ttccttatgg agaaatccga acgtataaag
    22501 aagtagccgt tgctgttgat agtccgaaag ctgtgcgagc gattgggcag gcaaatcgaa
    22561 ccaatccatt gccaatcgtt attccttgtc atcgagtgat cgggcaaaat ggaaaactga
    22621 caggatatat gggaaacgca atagatgggg ttgaaatcaa acgccaatt
//
LOCUS       DACCYH010000044        21862 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.045, whole genome shotgun sequence.
ACCESSION   DACCYH010000044 DACCYH010000000
VERSION     DACCYH010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.045"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            62..616
                     /locus_tag="IVY85_001606"
     CDS             62..616
                     /locus_tag="IVY85_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8044628.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(789..1262)
                     /locus_tag="IVY85_001607"
     CDS             complement(789..1262)
                     /locus_tag="IVY85_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8044629.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1274..1810
                     /locus_tag="IVY85_001608"
     CDS             1274..1810
                     /locus_tag="IVY85_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8044630.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1850..2221
                     /locus_tag="IVY85_001609"
     CDS             1850..2221
                     /locus_tag="IVY85_001609"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8044631.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2505..3776
                     /locus_tag="IVY85_001610"
     CDS             2505..3776
                     /locus_tag="IVY85_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP8044632.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4021..5046
                     /locus_tag="IVY85_001611"
     CDS             4021..5046
                     /locus_tag="IVY85_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8044633.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5340..6449
                     /locus_tag="IVY85_001612"
     CDS             5340..6449
                     /locus_tag="IVY85_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8044634.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6532..7428
                     /locus_tag="IVY85_001613"
                     /pseudo
     CDS             6532..7428
                     /locus_tag="IVY85_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7444..8268
                     /locus_tag="IVY85_001614"
     CDS             7444..8268
                     /locus_tag="IVY85_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8044635.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8300..9610
                     /locus_tag="IVY85_001615"
     CDS             8300..9610
                     /locus_tag="IVY85_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP8044636.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9758..11017
                     /locus_tag="IVY85_001616"
     CDS             9758..11017
                     /locus_tag="IVY85_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8044637.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11027..11899
                     /locus_tag="IVY85_001617"
     CDS             11027..11899
                     /locus_tag="IVY85_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8044638.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11911..12741
                     /locus_tag="IVY85_001618"
     CDS             11911..12741
                     /locus_tag="IVY85_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8044639.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12781..14964
                     /locus_tag="IVY85_001619"
     CDS             12781..14964
                     /locus_tag="IVY85_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8044640.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15025..16245
                     /locus_tag="IVY85_001620"
     CDS             15025..16245
                     /locus_tag="IVY85_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP8044641.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16242..17702
                     /gene="gtfA"
                     /locus_tag="IVY85_001621"
     CDS             16242..17702
                     /gene="gtfA"
                     /locus_tag="IVY85_001621"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP8044642.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17779..18885
                     /locus_tag="IVY85_001622"
                     /pseudo
     CDS             17779..18885
                     /locus_tag="IVY85_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19207..19416
                     /locus_tag="IVY85_001623"
     CDS             19207..19416
                     /locus_tag="IVY85_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8044643.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMASKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19409..19558
                     /locus_tag="IVY85_001624"
     CDS             19409..19558
                     /locus_tag="IVY85_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP8044644.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19593..20377
                     /locus_tag="IVY85_001625"
                     /pseudo
     CDS             19593..20377
                     /locus_tag="IVY85_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(20661..21746)
                     /gene="xerS"
                     /locus_tag="IVY85_001626"
     CDS             complement(20661..21746)
                     /gene="xerS"
                     /locus_tag="IVY85_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP8044645.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT         7428 a         3295 c         4209 g         6930 t
ORIGIN      
        1 gattatggta aaatatatac ataatcatta actcagaata tttttaagaa gggaaacaat
       61 agtgagctat aatgtacaac cgttaaggac aaatgaagag atacaagatt ttcagttttg
      121 gctaagaaga acaagtaatc cggaaagaga tagtttttta tttttgtttg gaatcaataa
      181 tgggttacgt atgtcagata ttataggatt aaaagttgga gatatacgag gcaaaagtaa
      241 gcctattatt gttgaacata aaacaggtaa gcgaaaaccg atttttatag ataatttgag
      301 agaagaaatt ttattatata ctgaaggtaa agaagaaaat gattggttat ttccgagtcg
      361 gcaacaaggt cgacatataa caagggatcg tgtctatcag atatatgcag atatagcaga
      421 aaaattagga cgagatgata taggaacaca tacattaaga aaaacttttg ggtatcacta
      481 ctacaagaaa actagagaca ttgctacctt aatgtttatt tttaatcata gctcgcaagc
      541 aattacaaaa aggtacattg gtataactga agatgaaata ggagcatcac ttcgtggatt
      601 taaactaggt gtataattta aaaattttta taaacagtaa agacaaagat taaaagctat
      661 ttttatctag aacgatttta tttcataaag ttcgcggaat aaaagctggg gttactggta
      721 cttggtatag gttcgtgtta tataaactaa gtaattaaat aacattttta ggttggaaaa
      781 ggatgaaatt aaaaaaagag aatcaatacg gtctcaaaaa cgcaaacgct gcggaataag
      841 aaaaatactg ataactcaac gtttagcaga ctatctaata gggatagtct gcttttttcg
      901 tttttgacca tacttttgac cttctgcaaa cttccttgct tttcctctgt agaactcatg
      961 aaatctgcaa aacgaattgc cgtttcctct gtctgctttt tcgcgatatg ggtgtatata
     1021 tcatccatag tggttttaat atctgtatgt ccaagtcgaa catacacttc tttgacagac
     1081 gcaccagatt caaagagcag actacaatgg gtatgacgaa aaccatgaag tgtgatatgt
     1141 tttaagttat tctctttaat gatgattttc aaattgtgat ttacatagtc taaataaagg
     1201 ggcttatttt cttctgatga aaacaaaagt tgttcaccat cttttatagg tttgattctc
     1261 atttttagat attatgcccg cacgtattca tcacatttct attgtgaatc gctttattcg
     1321 accgacattt gacttttatc ataatatact cggtttaaaa ttactaatga aaacaattaa
     1381 tcaagacgat cataccatgt atcacctgtt tttttcagat aatcaccacc gtgtgggaac
     1441 tgaactgact ttttttgaag tacaagaagg gaacaaccag ttttttggaa ccaatacgat
     1501 tgaacgaacg atactaaaag ttccttccga agcatcattg catttttggg aaatatattt
     1561 tgaaacacaa ggcgtttgcc attatggaat cgaatcattt tctggacgac ctatcttacg
     1621 ttttgaaggg ccagatgcaa cacgattagc tttagttccc ttacgcgaat ttgaagatat
     1681 tgatgattat ttcccgtatg tcacagacca gatccctaca gagcatgcca ttttaggaat
     1741 tgatgccatt caactacgcg tacaatattc cgatgcgact gaacgagagt tattgtcggt
     1801 aggctggtag cacaaagaat acgtcagttt cttttcgggt catcaacgag tgacaatctt
     1861 agaaaatcaa gacactcagt tctatcaaga agttcagatt attcaagatc gtgagaatcc
     1921 agttgccatt gaagggattg gtagcgtcca tcatgttgcg tttggcgtag aaaataaatc
     1981 tgatttacaa cgcatcgaca aacaattaca agagcgcaat tttataaatt caggaataaa
     2041 agatcgtgaa ttttttgtat cactttatta tcgtgaccca aatcaattat tgattgaaat
     2101 tgctacaggg gaaggaaacc tagatgcaaa agcgtatgaa aatcaaagtc cacgctttga
     2161 agaaattcct ttatttttac cgcagtactt aaatttcatt cgtgaacaag tggagcaata
     2221 attagctaat caaatccatg acgaagagta agaggttgac tcataagtca aaaaataaag
     2281 aaaaacgcat caaatccatt ctaaagaatg ttgatttgat gcggttccta tttttataaa
     2341 ctatcatttt cgactaataa aactacttat gagtcagcct ctcttttttt cttgattttt
     2401 atacctcttt ctaggtaaac taaacatctg ccaaaaatac cgtgtctcat tgaaacattc
     2461 tcaactattt caaaataaag gggagtacca aatgaacgag tatattgaaa ttatccacgc
     2521 aagagaaaca acttaaaaat gtcagtctca aaattcccaa aaataaaatt agtattttta
     2581 ccagcgtatc tggttcagga aaatcatcga ttgtttttga tactattacc caagaagctg
     2641 gtcgtcaact gaatgacacc tatgatagtt ttacccgttt atttttacct aaatacagtc
     2701 gtccagaggt tgatgagatt gataatcttt ctacggcgat tgtggtcaat caaaagaaga
     2761 ttggtggcag tgcgcgctca acgttaggga cgattactaa catcaatgcg ttatttcgtg
     2821 tgttgttttc atgttttgca acacctagtt taggatatgc aaatgcttat tcatttaatg
     2881 atcctagtgg aatgtgttct gtctgtcagg gaatcggtaa gacgattgca tttttaatag
     2941 gattgcttta tttttcggct attgataatg ctgattttgt attttctgat ccatttaccg
     3001 cagaagtttt gtctggaatt gaggaacagg caaagaatca tggttatttt gtattggtgc
     3061 ataatgttaa ttcagcagaa gatgttaaaa ctattcagag aaattggcgt tttgaggggt
     3121 tcattgttgt tggtgtacgt gtaaaagaat ttttggaatt aaataacgtt tttgatggtc
     3181 ctgttgttta tattgatacg catctttcaa aagagacatt aagagaactt gaacaacaaa
     3241 tgaatcgttt atttgtaaat acaaatgatt accaagctag tacattggca gtggaacatt
     3301 tgattcatta tggtcatgag aaaatagcgt ttctttcttt tgactacatt gtaggtgaac
     3361 caagtgttat tcaagaacga catcaagctt atatcgatac attaaagaaa tatcatttaa
     3421 cctttaaatc gcattggata tataacaact ctgaatttga gcgaatttat caagaattag
     3481 ctgaatttac tgcaattgtt gttactggag attatttagc ggctaaatta gtaaaatttt
     3541 taaaaaataa gaataaacga gtgatggaac aattatcgat tattggattt gatgatatta
     3601 cttttgcaga attaatggat cctgcgttga caacaatacg actaaatcca ggtaacaagg
     3661 gggcgattgc ttttcaccaa ctgattggac tggccaaaca agaagaaata aaaaatcaag
     3721 tactactatt agagggagaa ttgatttctc gagactctgt tcacaaaatc aaataaaaaa
     3781 gctctgtgaa ataagtatag ttaacaataa aatatccgaa cgatagagag aatgaactgt
     3841 gatctagcag ttcctcgtcg caaattcact agaattagca ttatttactc taaattgttc
     3901 ggatattttt tcttgttttt aattatgatt acagtgccta tttttaacga aatttgctta
     3961 ttttcaaaat gtatgattat atgttagtaa acactttcaa atatgaaaag aggagctatt
     4021 atggtaggaa ttagggatgt tgcaaaacgg gcgggtgtgt ccattgcaac agtatcgcgc
     4081 gtgctaaatg aggatccgac actttcagta accgatgaaa ccaaacgaaa gattgcagaa
     4141 tcggttaatt tttatcatta tgtcaaaaaa agtcccgttg caaaaaattt aacaaatatt
     4201 gcattaataa cgacagtttc tgaagtagat gagttggaag atccctattt tcgaacgatt
     4261 cgcagaggga ttcaagtcga agcagaacaa agcaaagtca atttgaagaa aattattcgt
     4321 ttgtcagaaa caccgttgga gcaagacgat ttgctaaaat gtcaaggtgt gttgattatt
     4381 gggcaggtgc tatcttctgt gattgaagaa gtatcagcaa ttaattcgaa tgtcgtagtg
     4441 attgatgacc ctagtattga agctgaagta gatgcagttt atactgattt aacaaaagca
     4501 acgaatcagc atttagatcg attgtataaa aaaggacacc gaaacattgc gtttatcgga
     4561 ggaggacgtg ttcgtttaga tagccaagga aataaacacg tgtcagatga tgattatcga
     4621 aaaatagcct atgagacgtg gatgaaacaa aaaggattag aaaaatatag tcaaatttat
     4681 ttagaaggct ggaccacact tgacggaatg caggcagcag atttactttt gaaaaaacat
     4741 gctaaagata agttgccgac tgctatcatg gtaggaaatg atcctattgc tgtgggggtt
     4801 tatcgttcac ttcaaaaaaa tggtctacat attccagaag atatttcgat tgtgagtttt
     4861 gataatattg aagttgctga atttttaaca cctgcattaa atacggtgaa tgtgaataca
     4921 gaagaaattg gaaaactagc agttcgcatg gttaatgaac gaataaaaga agttagaaat
     4981 gtttcgatca aagtgattgt ttctaataat attattgtta gagaaagtga aaaaaatatc
     5041 gattaaaaaa gtaaacatcc aaaaaagggt gtttactttt ttgtttacta atatttttag
     5101 taaaaaatct cgacaaaaca ttaagtaaac agttatggtg ttgttaacaa aaagaaaacg
     5161 cattcaaaac aggaggaatt aagatgaaac atttcaaaaa aatagcttta acagggatag
     5221 ctgttttaac tgggacatta ctcgcagcat gtggtggggg agatagtaat tctaaagacg
     5281 ggcaagtaac tttaagctat gcgagttgag atactgacca agctccagga ctaagaaaaa
     5341 tgttagatga atttgaaaaa caaaatcctg atatcaaagt agaaatggag acaacgcctt
     5401 gggatcaata ttgggttaaa ttagaagctg caacaacggg aggtaatatg ccagatgtag
     5461 taaccatgca ctctagcgaa tcctataaat atatgtcaca gaatgctttg atgggacttg
     5521 atgatgttat caaagaaaat gatattgaca tgggaaactt tactgaagga attgctgatt
     5581 tctatacgct tgaagacgaa ctatacgcaa tccctaaaga tgcttctgta gtggggctat
     5641 ggtacaacaa agaattattt gataatgcag gtgtcgatta tccagatgaa acatggacat
     5701 gggaaacact cagacaagct gcaattgatt taacagatga atcaaaaggc atctatggtt
     5761 ttgcatcaca tattggaaca gagactggct actggccatt tatttatcaa aatgggggag
     5821 aagtatttgc cgatgacaat acaaaatcag gtttgaatac cccagaagca caagaagctt
     5881 tagcttttta tcgagatatt gcacttgttg atcatgcttc accaacaatt acagaattac
     5941 aagatcctga taagttaagt cgttttcaat caggtagatt agcaatgatg attgaaggaa
     6001 actggtttgc accgggattt ttgaaaaatg actatctagt tgaaaatgtt gcttctgccg
     6061 tgttacctaa gggaaaagtt aatgcaacaa ttacaaatgg acttggttgg gctgccagtg
     6121 cgagtacaaa atacccagaa gaagtgaaaa aactaatggc atttttagct agtgaggacg
     6181 ccaataaaat ccaagcggaa acgggggcat caatcccagc actaaaaggc tttgaagacg
     6241 agtgggctgc ttcttcagat acaattgatt tgagtgcttt tggaaaaatg ttggagtatg
     6301 gagtccctcg tccatacaat aaagatggat tacgagctga acaagcagaa gttcaaatta
     6361 ttaaccgtat tctgtctggt gaagtaactg ttgaagatgg aaccgctcaa attgaaaaag
     6421 aagtcaatgc gatattgagc ggaaattaaa ttagttaatt atacaatata caggcgaaag
     6481 gattctattt cgtctgtata ttaatggatg aataccaaaa agggggatat tatgaaaaat
     6541 cgaatttttg gaaaatatcg taaagatcaa gtgatatggg gctatatttt tatttttcca
     6601 acggcattag gattaatttt attaaatata tggccagcaa ttcaaacagc aattttaagt
     6661 tttcaaaaaa cggtagggtt cggtgatact gaatgggtcg gattaagaaa ttaccaaaaa
     6721 atgtttgcag acggtgaagt ttttcagtct ttattaaaca cattaattta tgcagtggtt
     6781 tcgataccta gtattgttgt attatccttg ttggtggcag ttttaatgaa tagaaaagtt
     6841 aaaggattgt cgatttatcg gacaatttat tttctaccag tcgttgctgc accatccgca
     6901 gtcgcaatga tttggcgctg gatgttcaat aatgattatg gactgattaa taacatgttg
     6961 gctaaaatag gtctcgatgg accagcatgg ttaacggatc catcgattgc gatttattct
     7021 attatcattg taggagtttg gagtgctatt ggttataaca tggtgttatt actagccggc
     7081 ttataagaaa ttcctaaaga ttattatgaa gcagcggaaa ttgatggtgc tagtccaatc
     7141 cagcagtttt tcaacattac gttaccatta gttacaccaa cattattttt tgtagttgta
     7201 acaacagtta tcaatgcttt ccaagtattt gatgttattt ttatgatgat tactcctaat
     7261 agtaccgcca tgtttaaatc acaatcatta gcctatttat tctataaaca ttcatttatt
     7321 ttaaatgaca agggatatgg ttcggcaatc gttatgttcc ttttagtgat tatattaatt
     7381 attactgcaa ttcaaatgaa gttgcagaaa aagtgggtaa actattaagg aggctatcaa
     7441 attatgaaaa ataaattttt ttcaaatgcg ccaatccata tcatgcttat tttaggtggt
     7501 attgcaatgg cattaccttt tatttggatg attttaactt cagggaaaac gttggttgaa
     7561 tctacccaaa ttccacctac aattttccct gagaaatttc agctaagtaa ctatcaacaa
     7621 gtacttgatt tattaccatt catggctttt tatatcaata catttttaat ggttgttgga
     7681 cgagtgattg gctcggtttt attttcagca atggctgcat atgcttgtgc gcgtctaagt
     7741 tttccaggga aaaatatcct tttcagctta gtacttattc aaatgatgat tcccggttcc
     7801 atttttatta ctccacagta tttattagca caaaaattgg gtattttaaa tacaattcca
     7861 gcattaatat taccaggggt agttagtgcg tttggtacgt ttttattaag acaatttttc
     7921 ttaggtttac ctgatgaatt ggaggaagcg gcctatttag aaggtgccaa tatttggacg
     7981 atctttttca aaattatgtt gccgcttgct aaatcaggat tggttgcact tggaatattc
     8041 acagcgttgt ttgcatttaa agatttaatg tggccattga ttgtcaatac ttcaattgat
     8101 aagatgcctt tagctccggg attagcaaac ttacaaggac aatatgccac gaacttccca
     8161 caattaatgg caggttcgtt attagcaatt tggccaatag ttgtgatatt catcgttttc
     8221 caaagaaaat tcattgaagg aattgctaca acaggtagta aattataaaa ctattaatta
     8281 gtgattggag agtaagtaca tgacaaaaat tacatttatt ggtgcaggaa gtactatttt
     8341 tgcaaaaaac gtattaggcg atgcgatgtt aactccaagt ctacaagatg cacatattgc
     8401 attgtacgat attgatgaaa aacgactaaa agaatcaaat ttaatgctta aaaatattaa
     8461 taaaaattcg aatgaaaatc gtgctgatat tgtttcttat tatgatcgca aagaagcgtt
     8521 aagagaggct gattttgtta tcaatgctat tcaagtgggt ggttataaac cttcaacagt
     8581 gattgatttt gatattccta aaaaatatgg gttgcgacaa actattggtg acaccatggg
     8641 gattggtggg atttttagaa ccctacggac actaccagta atgttagact ttgctaaaga
     8701 aatggaagaa gtttgtccaa atgcttggtt attgaactat acaaatccga tggcgatgtt
     8761 aaccatgggt gttttaaaag cgacgagtat taagactgtt ggtttatgtc atagcgttca
     8821 agtatgcgta ccagaattgt ttgagcattt aggaatagcc gatcaatatg acttaaatga
     8881 ctttcaatgg aaaattgcag gcattaatca tatggcattc ttgttggaaa ttacaaaaaa
     8941 tggggaagat ttctatccaa caattcgtca gttagctgca gaaaaaccaa atccacatcc
     9001 agattctgta cgttttgaat tgttgaaacg ttttggttac tatattacag aatcaagtga
     9061 acacaatgca gaataccatc catactttat taaatcaaaa tacccagaat tgattgacga
     9121 attagggatt ccgttggatg aatacttacg tcgttgtgaa caacaaatta atgattggga
     9181 aagcatgcga gaggacattg taaataatgg ggagttaaca catacgagaa gccgtgaata
     9241 tgcttcatat atcatggatt ctattacgac aggaactccg tcagtgattg caggaaatgt
     9301 cttaaataaa ggcttaatca caaacttacc gacagatgtt tgtgtggaag taccttgttt
     9361 agtcgataaa aatggcgtgc aaccaaccta tgtcggtgaa ttgccaacac aattagctgc
     9421 tttaaatcgg acgaatatca atgttcaaga actgacagta gaagccgctt taacactaga
     9481 taaatcaaaa atttatcaag cagcgtattt agatccacat ctatcatctg aattgtcttt
     9541 aacagatatc aaaaatttag tggatgattt aatcgaagca catggggact atttacctga
     9601 atataattaa aaagaagctg aaagagtaag gaattttcaa ttccttactc ttttcttagt
     9661 taaatttagt aaaaatattg acaaaacact tagtaaacag ttacattagt gttaacaaaa
     9721 agaaaacgca ttcaaattgt ataaggagga gcataaaatg aagtggtcta aaaaaattat
     9781 tttagcttgt atagcactag gagcaacgat ctctttgact gcttgtggaa aaaatcagga
     9841 agatgcaagc ggtaaggtag aaatagagtt ttttaatcaa aaaaaagaaa tgactcaaac
     9901 gattcaggag attgctaaag attttgaagc aaaaaaccct gatattcatg taaaagttgt
     9961 ggatgtaccg aatgctggtg aagtaattaa aacacgtatg cttgcaggcg atgtaccaga
    10021 tgtcattaat ctttatcctc aaagtataga attgaaagaa tgggcgaaag ctggttattt
    10081 agaggactta accgaagaac cgtatttaga gaatatcaaa aatggatatg cacaacgttt
    10141 tgctattgag gatcgtgtat atagcatacc attaaccgct aatgtctatg gtttttacta
    10201 taacaaaact gcttttgagg aaatggggat tcaagcacca gaaacttggg cagaatttga
    10261 aaaaatagta gctgagatta aagatcaaaa caaagtaccg tttgcgattg caggagcaga
    10321 aggctggact ttaaatggtt atcaccaact agctatagcg actgctgctg gtggtgaaga
    10381 ggaagcgaac aatgtttggc gtttctcaga agttaatgga attaatgctg aaagcaaaga
    10441 aatgcaaaaa gattttaatc gattagattt gttaagagaa ccaaaagctt tgcaaaacaa
    10501 ctggcaaggt gccggttata acgatacagt agttactttt acaaaaggtg atgcgttgat
    10561 tatgccgaat ggttcatggg caatgccgat gattcattca caaaatccag attttgaagt
    10621 aggaactttt ccttttccag cagatgaagc aggtcaaagt ctaacgattg gcgcaggaga
    10681 cctagcctta tcagtttctg caacttctca acataaagaa gaagccaaac gttttgtaga
    10741 atatatgaca acacctgaag cgatgcagaa atattatgat gtagatggtg caccgtgtgc
    10801 tgttgagggc gttatcgaga atactcagga ttcaccatta agtggtttaa ctgaacttgc
    10861 ttttactgat cgtcatttag tttggttagc caaagattgg aatagtgaaa atgattttta
    10921 taccttaaca acaaattatt tacacaatgg caatcaaaaa gccatgatta acgcattaaa
    10981 tgctttcttt aatccaatga aagctgacgg aaggtaggaa aataaaatga aaataaaaga
    11041 aattttaaat cgttactggg caatcatttt tgtagcaatt ccaattattt tgcagatgat
    11101 ttttttctac tttccaatgg ttcaaggtgc tttttacagt ttaactaatt ggactggtct
    11161 gacttataat tttgattttg tgggtttgaa caattataag attctttttt ctgatccgaa
    11221 gtttatcaaa tcgattggct tcactttaat tgtgacgctt tgcatgattg ctggacaaat
    11281 tggttttggt attttgattg cgcgggcgtt gaacgcaaat attaaaggga aaacattctt
    11341 tagagcatgg ttcttttttc cagcggtctt atcgggcgtg gttgtttctt tagtatttaa
    11401 acaaattttt aactatggtt taccaaaaat tggtagtatg ttaggcattg aatttttaca
    11461 aaccagtctt ttaggaacaa catggggagc gatttttgca gtaatttttg ttttactttg
    11521 gcaaggaatt gccatgccag tcatcatttt cttagcgggt tttcaaagta ttccacaaga
    11581 aattattgaa gctgctagaa tggatggggc gaatgataag caaatttttt ggaatattga
    11641 aatcacctac ttattaccaa gtatttcaat ggtatttatt atggcattaa aaggtggctt
    11701 aacagcattt gatcaaattt ttgctttaac tggtggtgga ccaagcgatg ccacaacatc
    11761 tttggggtta cttgtttata attatgcttt taaaaacaat cagtttggtt atgcgaatgc
    11821 tattgcttta gtcttattta tcttaattgg gattgtttca ctggcacaat taaaattatc
    11881 gaaaaaattt gaaatctagg aggtttccca atgaaaacat caaaagaaaa aaatatttct
    11941 aaatacttgc ttttattatt agggggcttc atgattttag ttcccttagt ttcaacgcta
    12001 ttcggttcat ttaaaacaac acccgatatt atgaataatt tctttcgttt tcctaatcca
    12061 gcgactttgg aaaatttccg aagattgtta gcggatggaa ttggtcatta cttttggaat
    12121 tcggcaatca tcacaatcat tgcagtagtt gctgtgacag caattattcc tatggctgct
    12181 tattcactag caagaaaaat gactcgtggg aaagcatacg gattgatgta tttattttta
    12241 attttaggaa tttttgttcc tttccaagta attatgattc cgattaccgt tatgatgagt
    12301 aaaattggtt tagcaaatat gtggggatta atcttactct atctcactta tgccgtacca
    12361 cagacactat ttttatatgt tggttttatt aaactaaatg tgcctatcag tctggatgag
    12421 gctgctgaaa ttgatggggc tagtcacttt acaacttatt tcaaaattgt tttcccaatg
    12481 ttaaaaccaa tgcatgcgac gactattatt cttaatgctc tatggttttg gaatgatttc
    12541 atgctaccat tattaatttt aaatcgtgat tctaatatgt ggacactacc attgtttcaa
    12601 tataattata cgggacaata ttttaacgac tatggtccaa gttttgcttc ctatacagta
    12661 ggaattgtca cgattacact agtttatctt gctttccaaa gaagtattat agctggaatg
    12721 agcaatggag cagtgaaata accaataaat tattctcatt ataaaggagt tcataaactt
    12781 atgattatat ttgacaatca cacacaaaca tttcatttac aaaataaaaa aattagctat
    12841 ctattaggaa tcgaagaaaa taattattta acgcatcttt attttggaaa aaagattacg
    12901 cactattctg gtgggtatcg ctacccccga acagatcgtt ccttctcacc caacaatgca
    12961 aatgcgcaag atcgtttatt atctttagac acattaccat tagaatttcc aggatatgga
    13021 catggtgatt ttagagaacc ggcgcataat attaaattag gaaacggtag tcgtgtaaat
    13081 gattttcgtt atgatagcta cgaaattcaa aaaggaaagc ccagcttagc gggattgcca
    13141 gccttatata cggaagatga tgatgaagct gaaacgctga ttattcaatt gaaagacgcg
    13201 attagtggat tgactttaca gttgttctac acaatttatc aaaatcacag tgtaattatt
    13261 cgttctacgc aattagtaaa taatagtgat gaaacagtgg agattaaccg cttagctagt
    13321 caatcaattg attttccaaa tcgtccactc gagttaattc atttaaatgg tacctgggca
    13381 agagaacgtc aaatgactag agaaaaaatt catacaggga taaaagtatt agatagtaaa
    13441 cgcggatcaa gcagccatca acaaaatccg tttgttgctt tagtagatgc caaaacagat
    13501 gaatttcaag gagaggcata tggatttaat ttagtttata gcggtaatca cgaaacggtg
    13561 attcaaaaag atccatttga ccaaacacgg gtcattatgg gaattaattc atttaatttt
    13621 ggttggcaac taaaaactgg cgactcattt caaacgccag aagtcgtgat ggtttatagt
    13681 aatgaaggtc tgaatacgtt atcacagacg tatcatgaat tgtataatca ccgtttaatt
    13741 cgtggaaact atcgtttaaa agaacgtccc acgttaatca acaactggga agcaacgtat
    13801 tttgatttta acgaagaaaa aatcatgaat attgtcgatg atgcgaagaa cttgggcatt
    13861 gaaatgtttg ttttagatga tggttggttt ggcggtcgtg aaaatgactt cacttcatta
    13921 ggtgattggg ttgaaacaga aggcaaactt gcttgtggtt tggaaaattt agccaagcaa
    13981 gtgcatgata acgggatgaa atttgggatt tggtttgaac cggaaatgat ttctgaagat
    14041 agtgaactat ttcgagcaca tccagattgg gcattgggta ttccaggtcg tgaccgctca
    14101 attagtcgaa atcaatatgt tcttgatttt tcaagacaag atgttcgtga taatatctat
    14161 caacaaatga cggcgatttt tgatcgtgta ccgattgatt atgtgaagtg ggatatgaat
    14221 cgaaatatga cagaagtcta ttctgtcttg ttggactcaa cgatgcaagg tgaagtggct
    14281 catcgttata tgttagggtt atatgaattt ttagaaaagt taacaacgaa ttatccaact
    14341 attttatttg aaagttgttc gggtggaggc ggtcgttttg atgcaggttt cttgtattac
    14401 atgccacaaa cttggacgag cgacaatacc gatgccgtgg ctcgtctgaa aattcaatat
    14461 ggaaccagct tagtgtatcc aatttcaagt atgggtgcgc atgtttcagc agtcccaaat
    14521 catcaaacag gaagagaaac gagtttaact attcgtggca atgctgcgat ggctggtgtt
    14581 tttggttatg agctagatct aacgcaatta acttctgaag aaaaagcaga aattaaagaa
    14641 caagttgcct tttataaaaa gcatcgccaa cttttgcaat atggaacttt ttatcgcttg
    14701 gaaagtccgt ttgaaggcaa tgatactgca tggatgtttg tctcaaaaga ccaaacagaa
    14761 gccatggtct tttatttccg agtattagca gaagctgcgc atccattagt cacattgaaa
    14821 ttagcaggat tagcagaaga agcaacttat caaatggaag atgtaacatt tgctggcgat
    14881 gaattgatga atctcggttt ttatattgac tcagaactac atggtgatta tgcgacacaa
    14941 cgcttttatt tgaaacagat atagtagctt gaatttacaa tataagtgca acaccaaaag
    15001 agactcattc caaacgaaga aaatatgttt tatttacacg catttactaa aaagcagcct
    15061 gatttgaatt gggaaaatga agaagtacgt gaagaaatct atgcaatgat taactattgg
    15121 ttagataaag gactaggcgg atttagaatc gatgcgatat taaacatcaa aaaaaaacta
    15181 gagtatggca tctttgaatc tgatggagaa gatgggttag ccttcattgg gccctggatt
    15241 cttaatcagt cgggcattga agtgtggtta aaagaaatga gagagcggac atttaaacca
    15301 cataatagta tgacggtagc agaagccgat gttcctaatg aacgtttaga tgaatatatt
    15361 ggtgaagatg gctattattc gatggttttt gattttagtt atacggatat tgatgtacct
    15421 gaaactggtg aatggtttaa agatagccaa tggacatgga cggatatgcg taccaatatt
    15481 tttacgaatc aattagttac gcaagataaa ggttggggcg ccttatattt agaaaaccac
    15541 gatcaaccac gttcaattaa taaatatatt ccagaagaat ggatcaatga ttatagtaaa
    15601 aaaatgttgg cgacgctttt tatgttacta agaggtaccc cttttattta tcaaggacaa
    15661 gaactaggaa tgaccaacat agaaatggca tcattagaag attttgatga tgtagctacg
    15721 cattctcagt ataaacgggc attagaatat ggattggcac cagagcaagc attgaaagct
    15781 gtcaataaac gaagtcggga taattctcgc acacctatgc aatggaatac gcaaaaaaat
    15841 gccagattct caacttctga taatgtatgg ttaaaagtaa atcctaatta tccagaactc
    15901 aatgcagaag ctcagttaac caatcaacag tcggtattca attattatca tcaattaatt
    15961 aaattaagaa aaaatagtgt atacaaagac gtgctaatct acggacaatt tctaccagtg
    16021 aaagataaaa atgaacatat tttgctttat gaacgccaat tagaagataa aatcgtatta
    16081 gttatcttaa acgttacagc aacagaacaa gaatattatg tagatgaaca gtaccaacaa
    16141 gtattaatca ataattatga agatgtttta ttagatcaaa gtcgaaaatt gcgtttacga
    16201 ccgtttgaaa gtattgtatt agctaattat ggaggacaat tatgaaaatt aaaaacgaag
    16261 caatgctcat cacctatgca gatagtttag gaaaaaacat gggcgaatta aaacaagtct
    16321 tagacaaaca tttacaaggt gttattggtg gtgtacatct gttgcctttt tttccatcga
    16381 caggtgatcg aggctttgcg ccgagtgatt atacgacagt tgatccagct cttggaacat
    16441 gggaagatgt ggaagcatta ggtgaagatt attatttgat gtttgatttt atgattaatc
    16501 acatttctcg tgaatcaaaa tttttccaag atttcaaaaa aaaccatgag cagtcaccat
    16561 acaaagaaat gtttatccgt attcatgaat ttttcccaga aaatcgacca acacaagaag
    16621 atattgattt aatctacaag cgtaaagaca aagcgccgtt tcaaacggta gaatttgcgg
    16681 atggtacgac agaagaggtt tggaatacct ttggggaaga acaaattgat ttagatgtga
    16741 ccaaagaagt tgtgaaagaa tttattcgag aaaccatcaa agatatggcg aaccatggct
    16801 gttctttaat tcgattagat gcgtttgctt atgcgattaa aaaattagat accaatgatt
    16861 tcttcgtgga accagatatt tgggaacttt tagacgaagt gcgtcaagaa gcagcgaaat
    16921 atgacgtcga attattacca gaaatccatg agcattattc cattcaaatg aaaattgcca
    16981 atcatgatta ttatatctat gacttcgcct tgccaatggt cactctgtat tcactttata
    17041 gtggaaaatc agaacgttta gccaattggt tgaaaatgag tccgatgaag cagtttacaa
    17101 cattggatac gcatgatgga attggtgttg tggacgctcg tgatttatta acagatgaag
    17161 aattggatta cacctctgag gaattgtata aagtcggggc gaatgtgaag aaggtctatt
    17221 caagtgccaa ttacaataat ttagatattt atcaaattaa cagcacctat tatagtgcat
    17281 taggcgataa tgaccgtagc tatctgttag cacgtgcgat tcaagtattt gcgccaggga
    17341 ttccacaaat ttattacgtt gggttattag caggaaaaaa tgatattgaa ctgttggaag
    17401 caacgaaaga agggcgtaat attaatcgtc attactatga tttagccgaa attgaagaag
    17461 aagtgcaacg gccagtgatt caacaattat ttgcactttt aaaatttaga aatgaatctg
    17521 cggcgtttga tttggatgga acgattgaag tagcaacgcc atcagaatca gaaatagtga
    17581 ttacacgtaa aaataaaaac ggtagtgata gtgcaacatt aacggctaac ttggatacga
    17641 aagaatttat cattgagtct aaagatataa aaattttcac tagtgttgca ttaaaatttt
    17701 aacctattcc atagaacaaa aaatccttta tactagcact tggatgactt atccaagacc
    17761 aataaaaagg actcatacat ggataagtat aaaacaaata cagcatttaa taaatggttt
    17821 tctgccataa aattaggaaa actttctgtc aactcccaaa aatgtatttt tgattacaat
    17881 tcgtatcgta aaaaactgtc ttttgaagct gtgctcaagc tctttctcta cgcagttaat
    17941 gaagaaaaag aaagtttgcg tgatttgagt acagctctta tcgaccgatc gctccaacta
    18001 gaaaccgatg tgtcatcgat tagtcatacc caattgtctc gagcatttaa tgcacttgaa
    18061 cctaaagtgt tagaagaaat ttttcaacaa ctgttagaaa aagttcaaca tcaaatcaaa
    18121 ccaaccaagc gaaatcatct gtatctgatt gattccagta cgttttcgct tagtttaaaa
    18181 cgtcataagt gggcgacttt ccgtcaaaca aaatcaggtg tcaagttaca cttaaactat
    18241 tgttatatgg acgatactac gatgtatcca actgatttta ccttaacaaa tgcccatgaa
    18301 catgatctca atcaactacc agtattggtg aatcaaccag aggcaactta cgtgtttgat
    18361 cgtgggtatt aggactttga aaagatggat cagatgcatt gggatggcta tttcttcgtc
    18421 actcggatta aaaaaaacac caaagttcac gtgatagata ctctggaaac atcgccagaa
    18481 acagaaattc tacgagacga gctggttcgt ttaggatcta aaacctatct aaccgccaat
    18541 ttccgactag tcactgttca agataagaac ggacgtgtgt ttcaatttat caccaatcga
    18601 atggacgtct cttcaaaaga aatctctgat atgtatcacg ctcgttggca aatcgaattg
    18661 tttttcaagc acatcaaaca acacatgacc attaaaacct tcttttctca aagtgaaaaa
    18721 ggcgtgcaga atcaattgat tttaacgatg atttccgctc tcctcacgtt tctcatcaaa
    18781 ttggagacga aaacggaaaa atcagtgttc caaatcaaac gattctttcg ttatttactg
    18841 tttcagccct ttgaatgttg tctagaaaaa ttaattccca catgagaaaa aagaaatttg
    18901 gctcgttact tttggttttc agttgaataa cagataaaaa attttctgga aaaagtcatc
    18961 ctctgcgtca gcacagtttt ttttgactta ttttcagagg aaatagtttt cagaatattc
    19021 taaaatgttt tatgcaacac tagtgatatt ttttagaatt agatttctaa tgtgcatttt
    19081 ttgagtatta aggagcaagt atacaaagat ttataaagct gattaaattt gaataaaaat
    19141 atgcaggaac gctactattt ttagtagtgt tcttttttat atttgaataa aaaatgtggt
    19201 ggttaaatgg tggttataac aaaaaatgaa ttaatgaaat taggttatgg gaagtatcag
    19261 tctgagagaa tcatacgaca ggcaaaagca attatggctt caaaaagcta tacctattac
    19321 gaaaatagaa gattaggact agtacctgtt gaatctgtag aagaagtttt aggtatgaag
    19381 atagaggtag aggaggattt tcagaatcat ggttaatttt aaaaaagata aaaatgggaa
    19441 atatcatttt actgctagtt tgggattcga caaacgaaca gggaaaagag tacaaaaaaa
    19501 cgtagagggg ttaaaacagt taaagaagaa aaaaagcgta taccgaactt gtttctaatt
    19561 atggaaaaga agcagaaact tctcaaagta ctatgttgtt tgaggagttt ttttataaat
    19621 tattcctacc ttattatcaa gggaaagtag aagaaagaac atataaaaat cggttatcta
    19681 caatcaaaaa aacttttctt atttctttaa aatggagtta agagaaatac gtccaattga
    19741 cattcaaaag tggcaaaata atttattgag caaatataaa aattcttatg taagaaacat
    19801 gtatgggatt tttcagatgt cattagatcg agcagttgtt ttgggactac ttgaaaaaaa
    19861 tcctgcaaaa gttattggaa atgtgaagaa aaggaaaact acagttgatt tttggacaaa
    19921 agaagaattt acaaaagtta tttcaacttt ttatattgat gatttgtacc aacattttgg
    19981 ttttatttgt atgtggttac tttttatgac cgttatgcgt ttgggagaag ccactgcatt
    20041 aacttggaaa gatattgatt ttgaaaataa aaacttgcgt gtgaataaaa cactctatta
    20101 taagagagcc aaaacctatg agtttgtaga acccgaaacg gcttctagta ttcgtacaat
    20161 cgaactagac gacaatacaa ttaccttttt aaaagcttgg aaggagcgtc aagtttcaat
    20221 aggaggcatg tcgtttgtac tttcttataa tggaacacct actcagaaat atacaatccg
    20281 tcatattatg agaagacatg caacgatcgt tggtgttcat ataatccgca tacacggatt
    20341 aaggcatagt catgcttctt tgcttatatc acaataaaaa gatgcacttc gaattaaaga
    20401 aaggttaaaa catgctgatg tacaaacgac tttaggaact tatggtcatt tgtatctaaa
    20461 ttcattgaga gagataacaa atgatcccac agattatatc caaactaatt tttctaaaat
    20521 caaaccgaca cctatttacg ataataaaaa ataaatgata aacaaaagta ggatcacacc
    20581 aaaagaaaca cactagaaaa actataatac aggcttttct agcgtgttgt ttcttatttc
    20641 cgcctattta tacattatac tcataaagca tttaatgcat cgatcgtagc cgcattgtct
    20701 atatgggtat ataaagttgt tgtattggta cttgaatgtc ccaattgttg ggcagttaat
    20761 accaatgaat ttgtctgttg gtagagtcta gtagctaatg tatgacgcag tttgtgaggg
    20821 ctgacttgaa ttctaaagcc ttttgaatat tttgctacca tccgttcgat tgcaataccg
    20881 gatatacgct tggcttctcc ttggtaaatt gctagaaata aagctttttc gttttctgtt
    20941 actttatatc gttctttccg aatagctaga taattagcta aatattccat tgcgaatgct
    21001 gctatattga cagcatctct tttatttcct tttctagtta ctaccacatt attcttatct
    21061 aaagataggt catctaaatt aatatttata acctcggaga gtctcaatcc agatcctaaa
    21121 atcagtgcat tgatcgccag gtctctttct acatctcgtt gataatagat caaagctcgt
    21181 ttactcaaag tatttttata ttcattggca atataatcta gataattttg tgtatcctta
    21241 tctagaaata attttttctc aatggcattg gcacgtgcag ctaacgtagt tttatctgtg
    21301 atggttgcta cttgatccat aggattattg tctaagtatg gttttcctag aggattattg
    21361 gaacttttcg aaagatattt aaatagaacc tttaatgccg tgatatttct attgatagta
    21421 gaagtagata gacctaagtt attattatct ttgagcttag tttttgttgt ctcttttttt
    21481 acattttctt ttgtgcgacg tttcaaatga gatttgtaca agtttaggtc ataggcagtt
    21541 aactgtctta tcgaggtgat agaagtatca ggaacgatag actcactcag aagccattca
    21601 taaaagagct tataattttt tagatattct aaagtggttc gaggtgattg atctttatcc
    21661 tcacgatctt gaacaaaatc ttttatataa tcgggcatat gaagtatgat tgtatctatt
    21721 ttttgatata tttctgtatt tttcatgtca ttcatccttt caataagtag gggagaagag
    21781 tatctctata attggactat accaattata gaatgttttg tcaattggta tgctctaaaa
    21841 tttattaatt tgtttcaaaa aa
//
LOCUS       DACCYH010000045        21849 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.046, whole genome shotgun sequence.
ACCESSION   DACCYH010000045 DACCYH010000000
VERSION     DACCYH010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.046"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(566..2062)
                     /gene="lysS"
                     /locus_tag="IVY85_001627"
     CDS             complement(566..2062)
                     /gene="lysS"
                     /locus_tag="IVY85_001627"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP8044646.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2173..3177)
                     /gene="dusB"
                     /locus_tag="IVY85_001628"
     CDS             complement(2173..3177)
                     /gene="dusB"
                     /locus_tag="IVY85_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP8044647.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3178..4065)
                     /gene="hslO"
                     /locus_tag="IVY85_001629"
     CDS             complement(3178..4065)
                     /gene="hslO"
                     /locus_tag="IVY85_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP8044648.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4282..6393)
                     /gene="hflB"
                     /locus_tag="IVY85_001630"
     CDS             complement(4282..6393)
                     /gene="hflB"
                     /locus_tag="IVY85_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP8044649.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6483..7028)
                     /gene="hpt"
                     /locus_tag="IVY85_001631"
     CDS             complement(6483..7028)
                     /gene="hpt"
                     /locus_tag="IVY85_001631"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP8044650.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(7028..8473)
                     /gene="tilS"
                     /locus_tag="IVY85_001632"
     CDS             complement(7028..8473)
                     /gene="tilS"
                     /locus_tag="IVY85_001632"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP8044651.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8593..9063)
                     /locus_tag="IVY85_001633"
     CDS             complement(8593..9063)
                     /locus_tag="IVY85_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP8044652.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9109..9534)
                     /locus_tag="IVY85_001634"
     CDS             complement(9109..9534)
                     /locus_tag="IVY85_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP8044653.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9601..9870)
                     /locus_tag="IVY85_001635"
     CDS             complement(9601..9870)
                     /locus_tag="IVY85_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP8044654.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9881..11476)
                     /locus_tag="IVY85_001636"
     CDS             complement(9881..11476)
                     /locus_tag="IVY85_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8044655.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11491..15012)
                     /gene="mfd"
                     /locus_tag="IVY85_001637"
     CDS             complement(11491..15012)
                     /gene="mfd"
                     /locus_tag="IVY85_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP8044656.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(15029..15589)
                     /locus_tag="IVY85_001638"
     CDS             complement(15029..15589)
                     /locus_tag="IVY85_001638"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP8044657.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15942..16916
                     /locus_tag="IVY85_001639"
     CDS             15942..16916
                     /locus_tag="IVY85_001639"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8044658.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17086..18486
                     /locus_tag="IVY85_001640"
     CDS             17086..18486
                     /locus_tag="IVY85_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP8044659.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18564..19286)
                     /locus_tag="IVY85_001641"
     CDS             complement(18564..19286)
                     /locus_tag="IVY85_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8044660.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19365..19871)
                     /locus_tag="IVY85_001642"
     CDS             complement(19365..19871)
                     /locus_tag="IVY85_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8044661.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19994..21793
                     /locus_tag="IVY85_001643"
     CDS             19994..21793
                     /locus_tag="IVY85_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP8044662.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         6061 a         4759 c         3916 g         7113 t
ORIGIN      
        1 ttactttgtt cagttttcaa aggtctactt catcgagagt gtttcccgat tcatttttgg
       61 ctgccgtagc aacttttata tcatatcaga gagacaagcg attgtcaact cttttttaaa
      121 agtttttttc gatttcgttt ttcgaaatct ttcggcaact catttatcat aacttatcgt
      181 ttgttatttg tcaagaactt ttttgaagtt tttttcgata gaatcaagct tcgcaagtgg
      241 ttctttttta acgactcagt tatcttatca tgtcgttagt aacatgtcaa taactttttt
      301 gttatgtttt tgaataaata ttttcttctg tcttttggac aagtaataat ataccaacta
      361 aatttagctt ggtcaacgaa ttttttgtat tttttcaagt ttttttcaat aaacgattat
      421 tcataacttt tctttcttta tcgttcgttt tcgtcttctt taatgtctag aatagtcggc
      481 attagctttt gaactttcgt tattatttat tcctttctac acagcaaaag ctgcctaagg
      541 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
      601 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      661 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      721 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      781 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      841 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      901 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      961 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
     1021 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
     1081 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
     1141 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
     1201 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
     1261 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
     1321 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
     1381 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
     1441 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
     1501 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1561 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1621 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1681 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1741 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1801 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1861 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1921 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1981 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     2041 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     2101 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     2161 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     2221 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     2281 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     2341 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     2401 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     2461 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     2521 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2581 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2641 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2701 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2761 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2821 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2881 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2941 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     3001 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     3061 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     3121 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     3181 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     3241 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     3301 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     3361 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     3421 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     3481 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3541 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3601 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3661 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3721 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3781 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3841 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3901 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3961 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     4021 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     4081 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     4141 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     4201 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     4261 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     4321 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     4381 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     4441 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     4501 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4561 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4621 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4681 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4741 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4801 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4861 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4921 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4981 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     5041 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     5101 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     5161 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     5221 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     5281 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     5341 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     5401 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     5461 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     5521 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5581 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5641 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5701 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5761 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5821 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5881 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5941 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     6001 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     6061 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     6121 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     6181 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     6241 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     6301 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     6361 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     6421 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     6481 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6541 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6601 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6661 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6721 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6781 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6841 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6901 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6961 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     7021 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     7081 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     7141 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     7201 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     7261 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     7321 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     7381 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     7441 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     7501 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7561 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7621 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7681 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7741 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7801 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7861 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7921 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7981 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     8041 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     8101 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     8161 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     8221 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     8281 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     8341 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     8401 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     8461 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     8521 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8581 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8641 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8701 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8761 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8821 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8881 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8941 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     9001 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     9061 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     9121 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     9181 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     9241 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     9301 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     9361 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     9421 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     9481 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9541 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9601 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9661 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9721 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9781 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9841 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9901 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9961 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
    10021 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
    10081 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
    10141 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
    10201 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
    10261 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
    10321 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
    10381 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
    10441 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
    10501 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10561 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10621 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10681 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10741 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10801 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10861 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10921 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10981 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    11041 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    11101 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    11161 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    11221 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    11281 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    11341 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    11401 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    11461 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    11521 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11581 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11641 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11701 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11761 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11821 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11881 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11941 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    12001 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    12061 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    12121 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    12181 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    12241 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    12301 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    12361 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    12421 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    12481 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12541 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12601 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12661 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12721 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12781 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12841 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12901 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12961 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    13021 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    13081 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    13141 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    13201 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    13261 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    13321 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    13381 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    13441 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    13501 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13561 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13621 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13681 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13741 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13801 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13861 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13921 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13981 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    14041 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    14101 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    14161 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    14221 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    14281 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    14341 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    14401 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    14461 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    14521 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14581 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14641 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14701 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14761 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14821 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14881 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14941 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    15001 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    15061 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    15121 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    15181 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    15241 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    15301 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    15361 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    15421 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    15481 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15541 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15601 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15661 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15721 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15781 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15841 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15901 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15961 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    16021 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    16081 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    16141 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    16201 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    16261 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    16321 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    16381 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    16441 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    16501 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16561 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16621 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16681 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16741 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16801 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16861 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16921 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16981 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    17041 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    17101 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    17161 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    17221 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    17281 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    17341 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    17401 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    17461 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    17521 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17581 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17641 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17701 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17761 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17821 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17881 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17941 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    18001 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    18061 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    18121 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    18181 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    18241 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    18301 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    18361 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    18421 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    18481 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18541 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18601 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18661 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18721 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18781 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18841 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18901 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18961 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    19021 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    19081 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    19141 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    19201 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    19261 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    19321 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    19381 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    19441 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    19501 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19561 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19621 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19681 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19741 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19801 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19861 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19921 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19981 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    20041 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    20101 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    20161 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    20221 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    20281 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    20341 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    20401 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    20461 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    20521 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20581 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20641 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20701 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20761 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20821 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20881 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20941 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    21001 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    21061 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    21121 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    21181 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    21241 aagtacattc gcagaattga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    21301 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    21361 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    21421 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    21481 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21541 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21601 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21661 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21721 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21781 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21841 ctctttttg
//
LOCUS       DACCYH010000046        21386 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.047, whole genome shotgun sequence.
ACCESSION   DACCYH010000046 DACCYH010000000
VERSION     DACCYH010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.047"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            66..767
                     /locus_tag="IVY85_001644"
     CDS             66..767
                     /locus_tag="IVY85_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8044663.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1376..2392)
                     /gene="tsaD"
                     /locus_tag="IVY85_001645"
     CDS             complement(1376..2392)
                     /gene="tsaD"
                     /locus_tag="IVY85_001645"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP8044664.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2389..2853)
                     /gene="rimI"
                     /locus_tag="IVY85_001646"
     CDS             complement(2389..2853)
                     /gene="rimI"
                     /locus_tag="IVY85_001646"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8044665.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2860..3402)
                     /gene="rimI"
                     /locus_tag="IVY85_001647"
     CDS             complement(2860..3402)
                     /gene="rimI"
                     /locus_tag="IVY85_001647"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8044666.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3386..4210)
                     /gene="tsaB"
                     /locus_tag="IVY85_001648"
     CDS             complement(3386..4210)
                     /gene="tsaB"
                     /locus_tag="IVY85_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP8044667.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(4299..5279)
                     /locus_tag="IVY85_001649"
     CDS             complement(4299..5279)
                     /locus_tag="IVY85_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8044668.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5303..6787)
                     /gene="galT"
                     /locus_tag="IVY85_001650"
     CDS             complement(5303..6787)
                     /gene="galT"
                     /locus_tag="IVY85_001650"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP8044669.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6799..7788)
                     /gene="galE"
                     /locus_tag="IVY85_001651"
     CDS             complement(6799..7788)
                     /gene="galE"
                     /locus_tag="IVY85_001651"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP8044670.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8036..8203
                     /locus_tag="IVY85_001652"
     CDS             8036..8203
                     /locus_tag="IVY85_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP8044671.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8265..10076)
                     /locus_tag="IVY85_001653"
     CDS             complement(8265..10076)
                     /locus_tag="IVY85_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP8044672.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10073..10438)
                     /locus_tag="IVY85_001654"
     CDS             complement(10073..10438)
                     /locus_tag="IVY85_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP8044673.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10601..10996)
                     /locus_tag="IVY85_001655"
     CDS             complement(10601..10996)
                     /locus_tag="IVY85_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8044674.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11014..11976)
                     /locus_tag="IVY85_001656"
     CDS             complement(11014..11976)
                     /locus_tag="IVY85_001656"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP8044675.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(11976..12188)
                     /locus_tag="IVY85_001657"
     CDS             complement(11976..12188)
                     /locus_tag="IVY85_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP8044676.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12209..12907)
                     /locus_tag="IVY85_001658"
     CDS             complement(12209..12907)
                     /locus_tag="IVY85_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP8044677.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12927..13469)
                     /locus_tag="IVY85_001659"
     CDS             complement(12927..13469)
                     /locus_tag="IVY85_001659"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP8044678.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13601..14605)
                     /locus_tag="IVY85_001660"
     CDS             complement(13601..14605)
                     /locus_tag="IVY85_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8044679.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14602..15591)
                     /locus_tag="IVY85_001661"
     CDS             complement(14602..15591)
                     /locus_tag="IVY85_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8044680.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15588..16394)
                     /locus_tag="IVY85_001662"
     CDS             complement(15588..16394)
                     /locus_tag="IVY85_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8044681.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16560..17516
                     /locus_tag="IVY85_001663"
     CDS             16560..17516
                     /locus_tag="IVY85_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8044682.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17593..18111)
                     /locus_tag="IVY85_001664"
     CDS             complement(17593..18111)
                     /locus_tag="IVY85_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8044683.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18199..18348)
                     /gene="rpmG"
                     /locus_tag="IVY85_001665"
     CDS             complement(18199..18348)
                     /gene="rpmG"
                     /locus_tag="IVY85_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8044684.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18576..19022
                     /locus_tag="IVY85_001666"
     CDS             18576..19022
                     /locus_tag="IVY85_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP8044685.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19217..21112)
                     /gene="asnB"
                     /locus_tag="IVY85_001667"
     CDS             complement(19217..21112)
                     /gene="asnB"
                     /locus_tag="IVY85_001667"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP8044686.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6430 a         4547 c         3692 g         6717 t
ORIGIN      
        1 aactttacaa attctttaaa aaaagtgaca ataaatatag tgaacaataa ggagaggact
       61 ttttaatgcg tgttaataaa atcgattttc acttttcaaa actcagatat caacctgatt
      121 ttgaacattt ctcggatgaa gagttccaac agatcaagga acatactgtt cttcgttctt
      181 ataaaaaagg acagatcctt ttcgacgatg gtgatcccag acaacggttg tatatcttga
      241 ccaaaggact cgttcgttta gaacgttatg atgaaagtgc aacttattac tactatgact
      301 atgtgaatac aaataacctt tttcctatgt caggaatgtt tggagaagag acctacactt
      361 atacagctca agcgatgact gatattgaaa cattctacct tcctgtttcc tactatgaaa
      421 aattagcacg aaaaaatccc gaacagttga tctatatgat acaaaaaatt tctaaagtag
      481 ttgaaataca tgaattgcgt atccaaatcg gattgacctc tagcgccttt gaccgggtaa
      541 aacaaaattt attcattttg aaagaagaat tgggcgtacc aacaaaagat ggtcatatca
      601 ccattcctta tccgatcacc ttaaaagaat tagcagtgaa tagcggcacg acacgtgaaa
      661 ctgctggaca agtaatcaaa caactacgtg attccgggct gttagattat gacaaaaagc
      721 gttttacttt ctgtaagaca gctactactc cagctatgag agaataacgc gaacaaaaat
      781 aagcagtgac cctgataaat atcagggtca ctgcttattt tcctccctaa aacttctgaa
      841 agatccggca gttttagatc actggacaag actcaaagat tttctatgtg tgtttcagtt
      901 atgtaatatt cactgtttgc aaaaaatact gttagattaa ctagatgaag cgtcttgtcc
      961 tcatccgatc tagattattc gatattcctc gacaataaac gattactcag tgagggcaat
     1021 cagaggaacg gataatgata aataagctgt aaaaaatagc aaaataacaa taatgattaa
     1081 aagtatgtaa aaaatagggc tttgacaaaa acgtgtcaca gccctatttc gattaaacgg
     1141 tgtgtacctg tcaactcttc ggtcacaagt cataatgttc ataaacatga ggagctgttt
     1201 tttgaaattg ttccttgtga atcagagtta aacgacaggc ttacgaactc ttgattaaac
     1261 ggcgttcaaa agcttgcata cgcggtaata agaacagcca ggtcaaaaac atgagaagcc
     1321 gtttttcctg ggctgttctt attacttgat gcagggcagc tttttcacaa cctctttatt
     1381 cagataaaac caagcttgga tcagcattca actgatagct agcaaaattt cttttttgca
     1441 tttccacatg tgctgctgct ccaatcattg cagcattatc tccacaaagt cgaagagggg
     1501 gaatcactag ttctacctca gggagtttag ctgccaaagc atcctgcaat ccttcacgca
     1561 acccttgatt ggccgctaca cctccagcta taatcagttg cttgactgga taattttgac
     1621 aagctcgaag tgttttagta atcaatacat cgatcacact tgcttgaaag ctagctgcta
     1681 aatcattttt gtctagatct tctccacgct gttgtgcatt atgcaccaaa ttgataaatg
     1741 cactttttaa tccgctaaaa ctgaaatcat aattatcttc atggatcatc gctcgcggaa
     1801 aatggtaatt atcttttccc tgatgagcta gctgatcgat ttcttttccg cttgggtaag
     1861 aaagacctaa tactcttcca actttgtcat aagcttctcc tgctgcgtca tctcttgtct
     1921 ccccaatgat ttcatatgat ccatcttctt gcatatagac taattctgta tgtcctccgc
     1981 taacaagaag tgccatcaat ggaaactgga atggtttaac cagacgggct gcatagatat
     2041 gccctgccat atggtttaca ggaataagcg gcaattggtg tgcccaagca aaagccttag
     2101 ctgcagaaat accaatcagc aacgagccga caagtcctgg tccataagtg acagcgactg
     2161 ccgataaatc ttctgccgac acaccagctt ctaccagtgc atcttccaag cataaggtga
     2221 tttgttctac gtgatggcgg ctagctactt caggtactac gccaccaaaa cgtttatgac
     2281 tgtttatttg agaagcaacg atgttagata agatctctgt tccatttcta acgaccgcta
     2341 cactcgtttc atcacaactt gattcaatcg ccagaatcaa ttcttcattc atttcatcgt
     2401 ctccgttttt aactttctgc tcataatgat cgcatcttcc gtcggattgc gataatatga
     2461 tttccgttta cctaatatac gaaacttttc tgattcataa agttttcttg ctgcttcatt
     2521 agatagacgt acttctaaaa aaacatgcag cgaatctttt atttgttccg taatcagtaa
     2581 ttgtaacaaa tgccgtgcat gcccttttcc ttgttctgaa actgcaacag caatatttgt
     2641 gatctcgact tcatctatta ctttcgagta tccaataaag ccacagatct ggttatcttc
     2701 agctaaaagc agataatcag agtgaggctg ttggatgtct gccaaaaact gcattttcgt
     2761 ccaaggtgaa ccatgtggat aagcattttc gctaatcatc catacgcatt ctgccaagta
     2821 cgcttcatca aaatcttttt ttctgcaaat caactaatct cactcatttt ctaaaagttt
     2881 tatcatatcc aacgcatctt cttttgtttc agtgtaatat ctttttttga tcgttgttgc
     2941 ttgaaacccc aatttccgat aaagtctttg tgcatcttga ttactcatac gcacttctaa
     3001 tgacatcgtc tcacagcgat tcattatagc aaagttctcg atttcgtcaa taagcagcga
     3061 accgattcgt ttacgctgat atgccggtcg aacggcaata ttcgtgatat ggcaatctaa
     3121 accgaacgct cttgaaccga taaaaccaat cattcttcca ttatcaaaga tacctagata
     3181 tagatgagga agcggtgact tcaattctga tataaaggca ctttttgtcc atggcaattc
     3241 gccattatag acttctcgtt cgacatccag cagctctttg atatcttctg tagtcaattc
     3301 tcttagtacg taacgacgat cgatttgttc gactgttttt tctgtataaa tcattttatc
     3361 ttgaaaaaag cttctggcat acgctctaaa ttttttcaac ataactttcg tctccaggtg
     3421 tgtgcgtctc caaccatttt tcttctgctt ctactcgttt taaatagcga ggaagaaaag
     3481 cttgtttctc tgcaactggt tcaactaaag cacctaattg agccacagtt acaccacttg
     3541 gaatatccca tgctggaact gtattaatca tcgcactagg caatatttcc tttatttcag
     3601 agaagaattt gtgacaatca ctcccgacga aataaatagc ttcattcagc ttttgtagtt
     3661 gtgtgaacaa atcagaagca gaaatatgta catcttcaag tacagtttct aatctatttt
     3721 ctttccagcg gtatgcccca gcgtatacat tttttcttcg agcatcaaac atgggcacaa
     3781 taatattatc cacaccgatg caattagccg cgatggtttt cagactagat actcctacta
     3841 gctctttatc taatgtataa gcaatcgttt ttgctgtcgt tacacctata cgaagccctg
     3901 tataagaacc gggaccatca gaaaccgcga ttcggtcaat gtcttttgga cttatttgaa
     3961 gatctgcaaa caattggtcg attgcaggca tcagtgttac actgtggttg cgtttgatat
     4021 ttgtttgtat ttgtccaaga atttgttcgt cttctaccac accgatcgcc aaagtttggt
     4081 ttgcagtatc tattcctaat gtaatcatat atttgatcct ttctgctatg acattctcgc
     4141 cttccattgt agcatacttt tttattctga ggatgaaaat accaaaggca aaactagtac
     4201 aataatacat gattatctgc tggcgggcaa attttgcccc ttcctgaaac acaaaaaagc
     4261 tgcggtatca ctccattttc ccacagcttt tccatctttt atttcgttga ttctcgaata
     4321 atcaactctg tttccacaat aatctttctt gaaaccggtg ctttggaatt acttaattga
     4381 agaatcgtct ccactgctag ctctcccatc cattcggtat aaacttttac agtagaaagc
     4441 gagggcgtta catatttcgc tacactgaca tcattgaagc ccatcacaga aatatcttct
     4501 ggtaccacta aacctttttc ttgaatcgct ttcagtgcgc caatcgctaa tgcatcatta
     4561 gctgcaaaaa gcgcatcagg aacatgcgga ttcgttttta aatattgctt cactgcttga
     4621 tatcctgact cgacagaaaa atctgcttcg ataattggac cttcgtcgta gcctttttct
     4681 gccaaaactt gttgaaatgc cataattctg ggatctttta gccgctgatg gtttttcttc
     4741 gtatattctg ttccagcaag catagcgata tgttgcgctt tatgggagac aaagtgttca
     4801 atgacttttt gaacacttga cttaaagtcg acaactagag agtcgattcc ttgagaagtg
     4861 ccgtcagaat ctaccaaaag caaggcattt tgggatcctt tcatttctaa taactgagct
     4921 tcgtcgaatt ttcctagtgc gatcgttcca tctgccggaa gatcactaat ggaatcccac
     4981 gtttctttca ataaagtaac gtttgcttct tctgcctttt tttcgattcc taggcgaata
     5041 gataaataat aaagatcttc aagctcttcc gtatcgtcgt accactggat caggcgaagc
     5101 gtttgcttag tatttgctgc atttttacga tgtttcgtat aatttaagcg ttctgctgct
     5161 tcaaaaattt tttgctttgt ggctaagcca acggaaagat caggatcata gttgagaact
     5221 cttgaaacag tagcaggaga aactcctgcc aactgcgcaa tgtctttaat tgttgccatt
     5281 tgtctctcct ccttttttac gtttataaaa tcgcagtaaa tcgttcaaac gctgcctgtc
     5341 ctttttcatc tcgcttgaat actccagcgt cttccagtac acgtgcaaaa acctgaccta
     5401 cagatttttg tacgacatcg gtgaccgtat ctttcgtcaa atcaggatgt tcttttttta
     5461 attgttctgc ccacggacga tgatattcag ccatctggtt ttcttctcct aataagaaat
     5521 gctctacttc tactagttct ggtttcaaac gaggtggtaa aacagcaagc cccattactt
     5581 cgatcaaccc aatgttctcc tttttgatat gttggacatc tttatgtgga tgaaaaatcc
     5641 catcaggatg ttcttctgaa acattgttgt ctcgaaggac aaggtccaac tcaaagtaat
     5701 tgtcccttct tcgtgcaatt ggcgtaatcg tatggtgtgg tgtaccatct gctgaaaaag
     5761 ctctgataga tactgtttca tccgaatatg ctttccattt ttccaatact gagttagctg
     5821 cttcaataag caagtttttc tctgttgatt gcaaacggat cacagacatc ggccatttca
     5881 cgactcctgc gatgacttgc ggataatctt ctagtttgat aagtttttct atcggtgctt
     5941 ttgccatagg aaattcatga cgtcctgcct gataatgatc atgagaaaga atcgatcctc
     6001 caacaattgg taagtcagca ttcgaaccaa caaagtaatg aggcaagact tcagtaatcc
     6061 gaagcagtct agcaaatgtt tcctttgtga ttttcatcgg gcggtgttgt tctgataaaa
     6121 tgatcgaatg ttcattgtaa taagcataag gggaatattg gaatccccag ctttcaccgt
     6181 ctagattcat tcgaataatc cggtgattgg tgcgggctgg atagttcaag cgacctctat
     6241 atccttcgtt ttccatgcaa agcatgcagg taggatagtc tacttttacc gcctcgcgtt
     6301 ctgctgcgat ttgttttgga tctttttctg gcttagataa attgattgtg atttccagtt
     6361 caccatactc cgactctgcc ggaaatttga tgttttttgc gatggctctt gttttgatgt
     6421 agtcattttt cttacttaaa ttaaagaaat actcagttgc ttcctctggg cttttcgaat
     6481 agtgctgtgc aaagaatgca ttgacgactg aaggtggcgg tgtgagaaaa tccattaatt
     6541 gcgcttcaaa aatctcttgt tctgctggca attctgaaag aacaccattt tctcttgcct
     6601 tagcgacaag ctgatccaat aagtctacag attcttcctt aactggtctt acttcaatgc
     6661 tttctagagc atcttctcct accatcccta aaagacgatt ctgtaaatat aagcggtcca
     6721 tttccatcca tccgccagct tcgatcgcaa gtgtggcaaa atcacaaatc gtttgactga
     6781 ttgacatatc tcattcccct attcttcata accatttgga tggctgacat gccaatccca
     6841 tgctgttttg ataatcgctt tgatatctgt gtattcaggt ttccagccca gaatctctct
     6901 cgctttttca cttgaagcga ctaatcggct aggatctcca gctcttcttg gtgctatttc
     6961 tgctggaatt tcttttcctg tcacttctct agctgcttct agcatttcct tgactgagta
     7021 tcctttgctg ctgcctaaat tgaagaagtt gctttcattt ccttctttca agtattccaa
     7081 agctaaaata tgtgcagcaa tcaaatcttc tacttgaaca taatctctga tacatgttcc
     7141 atctggtgta tcataatcgt ctccataaac agccaaggct tttctttgac caagagctac
     7201 ttgtaaaatg attgggacaa gatgcgtttc tggtgtatga tcttctccga tagatgcatc
     7261 tgcttttgct ccggctacat tgaagtagcg caaagcgaca tagcgcatgc catatgcttg
     7321 gtcacaccac ttcatcattt tttccatcat caatttactt tcaccatacg gatttttagg
     7381 attagctggt gtatcttctg tgattggaga ctctttcggc tctccataag ttgctgcagt
     7441 agaagagaat acgattttat tcacgttgaa ctcgtgcatg acttcaagta agatttgcat
     7501 accgtatacg ttgttgttga aatacatcaa tggtttttcg acagattctc ctactaaaga
     7561 gcttgcagca aaatggatta ctccttcaat cggttccttt tcaaatacgg aacgtagaaa
     7621 ttctttatcc cggatgtctc cttcataaaa atgagcatct ggatgtactg cttgtttgtg
     7681 acctgtgagc aaattatcta ccacagctac ttgatacccc ttttgaacta gctggtctac
     7741 agcgtgcgaa ccgatataac cggctccgcc tagtactaaa attgtcataa aaaacactcc
     7801 tttgattagt aaaattgtgt ttgttgattc tatataattt aaaaaacgaa gtttactttt
     7861 atggtgaaaa caaacaaagt aaaatttcgt aaattcatat ggtaaattta taataaaaca
     7921 tttagtaaac attttcaagg gttttttgca aaggtttaca aacaaacgta aaactagcct
     7981 ttttttctct tttgttccat aatagatata acaaataata aatgaggtgg attcaatgaa
     8041 aaaatttgta tctggaatat tagtaggaag tctggcaaca gccgctgccg ttgctggttt
     8101 ggttgcttct gtgaaaaaaa cagtgatcga tccaattgac gaaaaagaag caatgatcga
     8161 agaaaaccgt aaaaaagcaa tgcgtaaacg cgtttctcgt taataattat ttaaaaatac
     8221 gcatgaagac tttctaaaaa acagataagt catcatgcgt atttttattt gtcagtcaat
     8281 ataagttgtg ctattttatc tgcctgtctc agattccatg gatctgtata aggtctagct
     8341 actgctgcag ttagccaatc cacaccttca acaggtattc catagcaata aaagtcagac
     8401 tggatttccc ctgaagcatc aatcacgcga ttcgtacctc gctcaacatc tatagcttct
     8461 gaatgataac ttcctgtatt atctgaaaat gaaaaagggc gaacaagttt gttttccttc
     8521 aaatttttta tgacaggatt acttgaacga gaaaagcttg tttgtggcaa tcgagcttca
     8581 atcaaatagc gactgctgat ttctttatct tgtagagaaa taacatagcg gttgtgcttt
     8641 tgctgaatat caaatggcgg ttcgatcaaa tgaaaaatac ccgcatctat cagtgctgct
     8701 aattctttcg tccgaatgat tggcggaccg atcgttaaaa agctatttag atgagtaaac
     8761 catccccata gtatttcttt gtattcctta gcagaaaaaa cgtgccattc aatagcctgc
     8821 cggattggat cacgccagtc ttttaatgca tcaaatgttg aagcaaatgc tcctgtgcaa
     8881 ttgccttttt ccgcttcttt tatctgccct tcaataaata ttcggctagc ttctgaaaag
     8941 ggctcattct gataggcaag aggagtttct aaatacgacc aatcccatct ataagggatt
     9001 agtgctggat gctttccgag acactcctct gaagagaggg agagaaaatc atgctcaaaa
     9061 tttttctttg gaatcggcaa gtgatattct tcgatgattt tcttgtaata aaacaactca
     9121 acgtcttttt tcagatagtc aaaaaatgtt tcgccagtca aaaatcccga ctgatgccat
     9181 tgctccaggt tttcttttgt tagtaaccgt ggccatgcca ttgctccgcc ttgcttttga
     9241 tttcttcctc gagggtaata agggagcccc cttcttgatc cactgtatac aatcggttcc
     9301 ttgcctgatg gatgatagac cagttttccc ttttcgttct cgaagcgccc tcctcttgat
     9361 actgtaaaca atccgacata atcaaaaaaa cttaagccta gacctcttaa aaagacattt
     9421 tctttaggcg gtatcttcga aagatctaca tctgcaggat tagaaggaag ctgatacagt
     9481 aaaacatttt tctttgcgta gtctacccac actctttctt cttctgtctc atcattttcc
     9541 cagtgaccgc ttgctaaaac aagctgatcg atagaatagc tttgctgatc tgtctgtaaa
     9601 agataaccat cttttctttt gcttactttt tttactaaag aataaacaaa ctgaatagaa
     9661 gctggaaatt cttcttgcaa ctttgagaaa aaccagcgtt gatagagtcc gtataaacat
     9721 cgagtagatt gctgatcttt ttccagacga ttgatttctt ctataattag ttgcttattt
     9781 ttggttaccg tctgttcgat ataatctgta gcaggtcctt tactccattg aaacaagttg
     9841 ggtccctgtg ttaccaatcc tccactggac aatgtttcat ctgtaaaaag cgtcacttgt
     9901 tgagaaacag aattcattaa taactccggt ggctgattca accgccaaac tcgacctcca
     9961 ggaccattgg gatcgaaaag aaaaagttca agagaatgtc tgtcctgatt attatggaga
    10021 atcctttctg ctacacttaa tcctctaggt ccggcaccaa tgatccctat tttcatttgc
    10081 atgcctccat ttcgataaat tttttcaata aaacagcatg gttctgatgt tcattttttg
    10141 caccataaac aaaagtaatg actggatgtt ctttcataag ttgactgagt ttaaaaatag
    10201 cttgcttctt ttcttcttcg aaaatcaatt cttgctggta tttctttgca aatgaagcaa
    10261 atcgttctgg tacatggtca aaagttttgc gtgcttcatt actaggtgca atttctttca
    10321 accacagatc taactgcgct ttttcttttg aaattcctcg tggccagaat ctatccacga
    10381 gtattctata gccatcctct ttttcagcat caaggtaaac acgttttaat ttgatcatag
    10441 ttgtttcctt ctttctacct tttcgcttag cttatatatg tagataatag cacaaaataa
    10501 aaaaaatgaa cggcacaact atccattgcg ccgttcatgt gatcttggtc aaaatctaat
    10561 gattgattcg gtttttaaag ctgtcataca atcatctgtt ttatactttt ttacttttga
    10621 tttttccaga ccatccgtca tatccaccct taagtaaata aatctcttga taacctgctt
    10681 tacgtaattt atttgctgta cggatgctca aacttttctt ttgatcgtaa atatagacag
    10741 gctggtcttt tcgaatagat cctaatgaat ctttcaaaac agtgtaaggg atgtttcttg
    10801 cacctaaaat atgcccagca tcaaacacat ctttttcgcg aacatcgatt acttgtgctt
    10861 ttctcattcc ttctcgaaat tcttcttctg tcaaagtcgt tgctgaacgt ttggccatta
    10921 ctcgtagata gacttcccaa aggatgattg caagcacaat caatagcaaa atgatattaa
    10981 ttacccataa aaccatgtaa tgcctccgtt tttttaagca aatttcaagg ctaatgaggc
    11041 agcaccaatg acaccagctt cattaccaag ctctgctaat ttgatttttg ttgattctct
    11101 tacttgcggg aaagtaaatt ctttaaaata tttctctaca cggctacgta aaaattctcc
    11161 tgctgccgat actcctccac ctaacacgat acttgacgga tttaatgtat tccccaagtt
    11221 tccgcaagct aaacctaagt agaagcatac gcgatcaaca accatcaatg caaacggatc
    11281 gtcttcttga gccatttcaa agatatcttt acttgttact tcttctccgt tgtctagcat
    11341 tgctttcagt tttgaatccc ctgcgtattc ttctgccaag aaacgaccta ggcgtaccac
    11401 acctgtagcg ctagatacag tttctaaaca tccgcgttta ccgcaagtac attcgaaccc
    11461 ttcaggatcc acagtgatat gtccgatttc tcctccagct ccgctcacgc cgtgaatcaa
    11521 atttccgcca gcaacgatac ctccgccaac acctgttcct agagtaacga atacaacgtc
    11581 tggatcgttt tctccagcac ctttccatcg ttcacctaaa gcagcaacat tcgcgtcatt
    11641 atcgatagcg aatttgattc ctgttcctgc ctcgatcagt tttttcacaa attgaacttc
    11701 tgtccagttt aagttatacg caccaataac cgttccagct tcgctgtcca cgcttcctgg
    11761 ggtacccatg ccgattccga taaagtcttc ggctttcata ttatataaat tcaaacgatg
    11821 attgattgat tcaatgattt caggaacgat atgcttacca tcatccaaaa tattggtatc
    11881 gatgctccat ttttgctgaa tctctccttc tggtgttaaa attgcaaatt ttgctgttgt
    11941 tccacctaaa tcaatcccaa taatcttctt gtccattaca aatttccttt ctttcggctt
    12001 tcttcaattc ggtgctctct ttttaatatg cgccacgctg tcatgtatgt atcatgatca
    12061 atcaaacggc tatcatacag attacgcaat tcgatagtca ttaattcaat gtcatagatg
    12121 cgtgcaccca catagataaa gatgccaaat tttttgaaca gctgctgtac atcatataag
    12181 ctctccatct ttaagcactt ccttcatatt atacaagtaa accatatttt tttaaaccca
    12241 gtaaaataca tattacaaaa agaaatacaa aaactagtgt agaaagaatc ctttggtgta
    12301 tcgaatagct tgtctgttgt ttcggtagac ccaaaacatt ccctaacact aatccgccaa
    12361 ttagcccacc gacatgtccc caaacatcta cagaggtatc cagaagacca aaaacaaatg
    12421 tcaacacaat aaataaaaga aaatgtctga ctagctgctg gatcgctggg ttatgtttaa
    12481 aatgaaaccc taagataaac aacgcaccga acaaaccgaa tatcgaagta cttgcaccac
    12541 cagacagaac attcgcttcg ttaaaagcaa agcttgctgt atttcccatt atcccgctga
    12601 acatataaat caagaaaaaa cgccaatggc catagattgc ctcgatttgc tgccccataa
    12661 aatacagtat cagtgagttc attacgaaat gcatcagtcc aaaatggatg aagattggtg
    12721 tcactaaacg ccaccattca ttgaagtgaa caagataagg accatacatt ccggcataat
    12781 aagggatctc aagtcgcgga aataattcca tcatgaggaa gaccgctgtc tgtatcccta
    12841 aaaaagcata ggtccataac ggtcgtctta accatgcacg caattttatt tgctgttgat
    12901 aattcatgaa aacctcccat ttcattttag tcaataaaca attgttggat cttctgatca
    12961 aagctctccg gttgccattg ttcattcaat tgttcacgaa aaacaaggct acatgttttt
    13021 ttatgaaaat tatttaaata ccggtcgtaa taaccaccgc caaaaccgat tcgataaccg
    13081 gctctattgt agataatacc tggtacaatg aggagatcga tcatctccgg caaatagagc
    13141 ttctctgatt gtggctcaag aacaccaaaa ctcgaacgta caagaacgga ctgttcatcc
    13201 atttcataaa acgccatctg acgtttcggc aaagttttag gtacaactat tttcttttgt
    13261 tctttttctg ctcgttggat aaccggctgt gtatccagtt caaattcatt cgataaagtc
    13321 gtccctatca cttttgctga cttccagtac tcgctggcga atagctgttt atagattcgc
    13381 tgttcttttt cctttttctt gccacttttg gccagatttt tcagcttttc gattcctttc
    13441 cggcgtaatc ttactttcaa ttggtccaaa aactcaccct tccttctctc ataaaatggt
    13501 ataggaaaaa aagaaaaata gcaatcgaac tttcaaataa atgcataaat agaataaaaa
    13561 gaagacctag aaatgattgg ccttcctttt ttaatgaatt tcaactgttc agcgcgtgtt
    13621 tttgtctgat aaaaagtgca tacagaaaat atggcgcacc aaaaagggca atcaaaattc
    13681 ctgtaggtac ttccccactt ggtagaatca ctctagcaac catatctgca ctaagcagaa
    13741 aaatggaacc gaacaaaccg ctcagaacaa aggtccagtt attttcttta cgtacgacta
    13801 gcttcgcagc atgaggagct agtagtccta caaaagataa actacctgtc accgctacac
    13861 taccgcttgc caaagcaaca gctaacaaca aaaggatttt ttgactttta gccacgttaa
    13921 ggcctaatcc ttgcgcgcgt tcttgaccaa atgatagcaa tacaatcctc tgtgcataaa
    13981 gtcctcctag cggaattaag ataaccgccc atggaagcaa tgcccaaaca taaggccagc
    14041 tcgcacccca gattgatccc gctaaccatg cattcacaaa tccataattt tccttagata
    14101 attggatcgt gcatagcaat gttaatgcag aaaggccggc attaacggca ataccggaca
    14161 ataagattcg attcataccc aaaaagccat ttcgatcata ggccgcaaga tagacacagc
    14221 aagcagctaa aacaccacct agaaaggcta caactagcaa tagaaacggc agcgaatagt
    14281 gcgaaaagaa acctaaataa agcatcacta aaaatcctgc tccggcatta atgcccaata
    14341 tactagaatc tgccaattca tttcttgtta ctccttgcaa gatataacca gaaacgccaa
    14401 gacaagctcc tgctaataaa ctgatcagta atcgaggaac tcgaaattct ctaacaatca
    14461 gcgccaaaga agatgattct ttccctatga acgtggcaaa aagctgatcc aggtaaataa
    14521 aaggcgtacc aatcattaaa ctacctatag acaggatgac aagtacaaaa agcaagaaat
    14581 agaacgtcca atatattttt ttcaccgttg ttccctcctt acttgtatca gtaaaaacgg
    14641 aacgccaatc aatgcaatga ctaatccaaa aggtgtttca aaagggggat tgatcatcct
    14701 cgctgtaaaa tccgccaaca taaccaatcc accgccaact aaaaaagaga caggtagaac
    14761 ataacgatag tccgttccaa ccattccgcg aacaactggc ggaaccaaca gcccaataaa
    14821 tgaaacaggt ccgactaacg aaacggcaat ggctgctaac actgcaacta gacagaaaac
    14881 aatcaaccgc gttcgtttag gatgacttcc aagagctaat gcatgtgttt cactcaaatg
    14941 aatcaaagat attgcttttc ctaatgaaaa agaaaccaaa acagcaagta tatataaagg
    15001 aaacaatact gctaattgtt tccatgtgat attggcagct ccgcctacaa accaaaaagc
    15061 aagatcctgc tgcagattaa agaattgggt aagtgcttgg ctgatagcag taaaaaaaga
    15121 actaatagct acgccagcta aaatcagctg taaaggattc atgcccattt tcgaaaactg
    15181 caaaaaacca aatacgagca aagttacagc aaaagaacct gccaatgaca ttagaaacac
    15241 agaaaaatca gaagctttag gccaaaaagc aaaactaagg gccattgcca acgaagcacc
    15301 cgcgttgatt cctagtaagc ctgaatctgc taaaggattt cttgtcactc cttgcatcaa
    15361 tgcaccgctc aatgcaaaac aactccctac aagaaatgct cccgccacac gaggcaaacg
    15421 aatatttcgt atgatttggt gactttgttt tgatctatca aaatcaaaca acgcctgcca
    15481 aaaatctgct ttttccacag tcgctgcgcc atttgaaaga gcaacaaaaa aaataatcaa
    15541 aagaacaaaa cagaccaaaa gcaggagtct atttttcttc atagtcctca taattgatcc
    15601 tcctgctctt ttggcaacaa atcataggtt agaaaaactg gtttatttgt tcttggaagc
    15661 gtgacaatcg ttgcatcaat ctcaaatatt tttttcatac actcttttgt gatcacttct
    15721 tcaggtgtac cttctgtgat caacttaccg tttttcattg ctaataaata atctgaaaaa
    15781 cgtgaagcat gattaatgtc atgaatcgac atcaagatcg ttgtttgttt catttgattg
    15841 atttcagtca ataactgtag gatctctaat tggtgtgccg gatctaaaaa tgttgtcggt
    15901 tcatccaaga tcaagatatc tgtttcctga gcaagcgcca tagcgatcca gacacgctgg
    15961 gtttgtccac cagacagcgt tgcgatatcc cgcatacgaa atccagctag acctgtagca
    16021 tccaacgccc actcaattgc ttcatgatct tgcgtgtcca tgccactgaa cagtttttga
    16081 taagggtatc gtccgtaaga tactacttct tctactgtca tacctgtagg gtgttcacta
    16141 gtttgtgcta ataatgcaat tttttttgca agttcttttg aactgtatgt agagatatca
    16201 gagttattga caatgatctt tcctgatttc acaggtagta tacgggtaaa acttttcaat
    16261 aaagtagatt ttccacttcc atttggccca atcaaactag tgattttccc ttcaggaatc
    16321 gtaacagaca tgttttcaat gatttttttg ttttcgtagc cggctgcaat gtcagctgct
    16381 cttaatctcg tcatttcacc catcctttcg ctgaaaatca ttctcaatta tgacgctgtt
    16441 tcaacatttc gtcaagcgct actttcatat ctatagccaa atgaagcggt tgacagcgtt
    16501 cgaaatatca ggaataataa ccaatgagaa agattctcaa ttatgttagg aggaaagaaa
    16561 tgaaattaaa aacaaaagta ttcacattat ctagcatatt gctgctggct ggagtactga
    16621 gcgcttgctc cccctcttca gataaagaaa cagctacaac tgccaattcg acgacttcat
    16681 ccaatagtac tcatacagtc acagatacac ttggacataa agtagagatt ccaaacgcgc
    16741 ccaaacggat catcggcagt tacttagaag actatctgat tgcactaggg gaaaaaccta
    16801 tcgcccagtg gactgttggc agtggttcca ttcaagatta tctgcaagat gatttaaagg
    16861 atgtcccaac gatttcttat gaccttcctt atgagaaagt gttaagcttt gagcctgatt
    16921 tgctgttgat ttcttcttcc gctactgtcg aaggcgggaa atacgaacaa tatagcaaaa
    16981 tcgcgccaac ttatgttgta aaaaacggaa cagatgtcac atgggaagaa caactaaaag
    17041 atgtcggcaa agctttggca aaagaaaaag aggcagaaaa aatcatttcc gattatcaaa
    17101 aatccatcaa gaaaactcga gaagaactag cagataaaat cgagaacaag acagctgcag
    17161 tgttgtgggt gaccaacaac tcagccttta tggtcagcaa aaatcggtca agtggaagaa
    17221 tcgtatacga cgatctggaa tttggtgttc ctgatctagt ggaagaggtc tccaaaaaag
    17281 ccacttctga ctggtctgct gtttcctctg aaaagctagc tgaattagat gctgactaca
    17341 tcatccttgt gaacagtgac aaagatgctg ccatgttcaa tgaaccaagc tggaagaatc
    17401 taaaagcagt gacagacaat catgtactgc aattcggacc tgaatctagt tggctatata
    17461 acggacctat tgccagtcag gaaatggtca atgatatcaa agaaaaacta aattgatttt
    17521 aactaaaaaa agcatcttca cgattagtcg tgagatgctt tttttcttat taacaaacta
    17581 gacgcataga ttttattctt cttttgaagc aaccctattt tttactcttt cccatttttg
    17641 aaaacgatgg gcatatagat tttcttgatc cgttatcccc tcatactcct ctataagggc
    17701 gaagtctgaa aaatcaatat tcccaatata cgtatcgcct tcaaacgtgc tatcgatcaa
    17761 tgttcgccag atccgaccag tgtaaggaag aagttcttga aaaatccgac taccacccga
    17821 aacatagata tcttctgaga tcgtttgact atacgacaaa agttcatcga tcgaatgcat
    17881 tacttccgca ttttctttcg tgcgaaaatt tggttgcgtt gtcaacacga tgatatggcg
    17941 ataaggaagt gaaagatccc ccattccttc ataggtcttt cttcccatca ctaaaattcg
    18001 atccattgta tgttcgcgaa agaaacgcat atcatttggc aatcgccatg gtaacaaacc
    18061 atttttgccg atcagaccat ttttatcttg tgcccacatg gaaataaaca tattatccgc
    18121 tccttatgat tttagatgtt ttgagataaa aaaagtctca attctttgac cagcaaggaa
    18181 ttgagaactt taaaaacatt attttgtttc acggtgcaaa gtaactttgc gttcgcgtgg
    18241 gcaatatttt tgtttttcca aacgatcagg attgttacgt ttgtttttgc ttgttagata
    18301 gttacgttct ttacaagaag tacactctaa agtgatgttt acgcgcatgt ttttccctcc
    18361 catttttaca agaatcctct aagatgactc tcagacttga ttatcttacc atgatttttt
    18421 ctagattgct agtgtttttc acgattttgt taagtaaaac tacttgacga taaatacaac
    18481 ggttttactt ctcaaacaaa atctttttct ttttgtataa atacccttac acttgtttat
    18541 atagattatg ttttttaata aagggggata ctaatatgcc atggaacatg aaggactttc
    18601 ctgcatcaat gaaaaactta gataaattga caagaaaaaa agcaatcgac atcgcaaatg
    18661 cattattaga tgaagggtac cctgataacc gtgcgatccc aattgccatt gatcaagcaa
    18721 aaaaatggta tgaagatgct agtgaatcag aaaaacgaac tttcgaaaaa gaaaaaaatc
    18781 caagcaagac ggatgaacac gatacgaatc ctcgtgctgg aaaactgctt gattcagatg
    18841 tgatcgtaga atatgaggaa gaacaatgga tcgtgaaatc taaaggggca aaaaaggcaa
    18901 gcaaccattt tgatacaaaa aaagaagcaa tcgaaaaagg aaagcaagta gcacaaaaca
    18961 aagaatcaac tttgttcatt tataaaaaag atgggacaaa agaaaaagaa atcgattatt
    19021 gaacatatgt aaaggggttg tgacgaaaat cgtgtcacaa cccccttatg ttcgaaacaa
    19081 tggtgttcct ctgccaactc cgaaccataa gtcataatat ctataaaacg ggaagagctg
    19141 ttttttgaaa ttgtttctta tcactcagtg ttaaacggta ggagaaaacc tcttttttat
    19201 tgatatttga ttttcatcaa acagtcacat agtcgattgc ttctgcttgc ggaatcgttt
    19261 catctacaaa gtagacttta taccatgtca agaaactgaa gattgtccag atttttcttt
    19321 gctcttcttt acgtccttcg tgatggtcat ctagcaactt catgattttt tcttgatcaa
    19381 aaaattcttt tgcaaaatct gcttcgaata atgctttgac ttgttcgtag ccgtgttctt
    19441 ctttcaacca agctttgatt ggcacaggga atcctaattt cacacgattc gaccattctt
    19501 ccggaagatg tttgttcgca gcttgtctga aaatatcttt tgtgttgttt tgattcaata
    19561 aaaacttact tggtatttgt tgagccaatt tcatgacttc gatatccaat aaaggtactc
    19621 ggacttccaa agaacttgcc atagaaagtt tatctgcctt taacaagata tcttttggca
    19681 tccattgatg aagatcgaca tattgcatct tattgacttc atctttgatt ccttctgttt
    19741 tctcgtaatg aacagtcatg atttcatcca cagatggtgc tgtttgatat tttggtgtta
    19801 acacttctga tgcttctgat tcttcgaaca ctcgagcatg accgataaaa tattcttttg
    19861 caggagcaag tgattcatat aaatggatcc gtccatggaa gtttttcatt tttccgattt
    19921 tacgtcctaa attatagcgt gttccttttg gtaatttttt caatccttga gcaatcacac
    19981 ggatgaattt tgaattcgta tggaatccat acgtttggta tcccgcaaac agttcatccg
    20041 ccccttctcc agattggact acccgcacac tttgtgaagc aagatttgct aaaaagtaca
    20101 aaggtacgca agaaggattt gaatcaggtt catctaagtg atactggatc aaagggaatg
    20161 ctttgaacga catgtctccg tcgatcactg ctgctgtatt atctaagtcc aatagttcgg
    20221 ttagttttct ggcttcaatc gcttcattat aggttttatc atcaaacccg attgagaaag
    20281 aatgatctgg tttcaataca gaggttacat aactggaatc aaccccgctg gataagaaag
    20341 aaccaacttc tacatcactg accgtatgtg cttcaataga agcttgaacg gtttcgtcga
    20401 ttttttcgat ccattcttgt cgggaatgcg tttcttttgt ttcaaaatct gcatcccaat
    20461 attgctgaat gtctattttt ccatctttat acgtataata atggccttca ggcaaacgat
    20521 agacatcttt gaagaatgtt tcgccgttta atggagaata ttggaaagtc atataaggtt
    20581 tcaacgcttc tttgttcaat tctttgttaa aatcaggatg aggcagaaaa cttttgattt
    20641 ctgaaccaaa cataaatgtt ccgttcattt ctgcataata atatggtttg atcccaaagt
    20701 ggtctcttgc accgaataac tcattctttt cattatccca aatagcaaaa gcaaacattc
    20761 cgcggatctt ttgcaataat tctgttcccc attcttcgta accatgcaaa aggacttctg
    20821 tatctgcatg tgtttgaaaa acgtggccag ctgcaatcaa ttcttctctc aaaggctggt
    20881 aattatagat ttcgccatta aaaataatga tctttgtccg atcttcattg taaataggct
    20941 gagtaccgcc ctctaagtca ataatactta atcttctgaa tcctaaagca acatgtttat
    21001 cgatatattc tcctgaacta ttaggtccac ggtgaacgat tcgatccatc attgtattaa
    21061 taattgtttt tttgttgtcc ttgtcgttaa caaaacctac aataccgcac atttaacttt
    21121 cacatcctaa tataaaatta ctgtttattt cattcatatg catcttatca tagaataaat
    21181 agcggacgaa tattttctct cttacaattt cgtcacgtga aaacaaaaga tgaaagtttc
    21241 tgtttttaca gaaaataaag tcccaatatc taatttacca tgttttcttt tttgaggcta
    21301 ccttattctc ttcgtgttaa aaaaatatta gtggatgcta tttccaaaaa aaccagcaaa
    21361 aaaagacacg acctaaagat cgtgtc
//
LOCUS       DACCYH010000047        20790 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.048, whole genome shotgun sequence.
ACCESSION   DACCYH010000047 DACCYH010000000
VERSION     DACCYH010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.048"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            4..1143
                     /locus_tag="IVY85_001668"
     CDS             4..1143
                     /locus_tag="IVY85_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP8044687.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            1136..1936
                     /locus_tag="IVY85_001669"
     CDS             1136..1936
                     /locus_tag="IVY85_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8044688.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            1936..2763
                     /locus_tag="IVY85_001670"
     CDS             1936..2763
                     /locus_tag="IVY85_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8044689.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            2741..3475
                     /locus_tag="IVY85_001671"
     CDS             2741..3475
                     /locus_tag="IVY85_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8044690.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            3575..4441
                     /gene="rfbA"
                     /locus_tag="IVY85_001672"
     CDS             3575..4441
                     /gene="rfbA"
                     /locus_tag="IVY85_001672"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP8044691.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            4455..5027
                     /gene="rfbC"
                     /locus_tag="IVY85_001673"
     CDS             4455..5027
                     /gene="rfbC"
                     /locus_tag="IVY85_001673"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP8044692.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            5049..6077
                     /gene="rfbB"
                     /locus_tag="IVY85_001674"
     CDS             5049..6077
                     /gene="rfbB"
                     /locus_tag="IVY85_001674"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP8044693.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            6175..7026
                     /gene="rfbD"
                     /locus_tag="IVY85_001675"
     CDS             6175..7026
                     /gene="rfbD"
                     /locus_tag="IVY85_001675"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP8044694.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            7060..9093
                     /locus_tag="IVY85_001676"
     CDS             7060..9093
                     /locus_tag="IVY85_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044695.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            9137..10417
                     /locus_tag="IVY85_001677"
     CDS             9137..10417
                     /locus_tag="IVY85_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP8044696.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            10627..11433
                     /locus_tag="IVY85_001678"
     CDS             10627..11433
                     /locus_tag="IVY85_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8044697.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            11445..12665
                     /locus_tag="IVY85_001679"
     CDS             11445..12665
                     /locus_tag="IVY85_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8044698.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSNGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            12655..14241
                     /locus_tag="IVY85_001680"
     CDS             12655..14241
                     /locus_tag="IVY85_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8044699.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            14280..16418
                     /locus_tag="IVY85_001681"
     CDS             14280..16418
                     /locus_tag="IVY85_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8044700.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(16786..17769)
                     /locus_tag="IVY85_001682"
     CDS             complement(16786..17769)
                     /locus_tag="IVY85_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP8044701.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            18147..19538
                     /locus_tag="IVY85_001683"
     CDS             18147..19538
                     /locus_tag="IVY85_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8044702.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            19554..20594
                     /locus_tag="IVY85_001684"
     CDS             19554..20594
                     /locus_tag="IVY85_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8044703.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            20613..>20790
                     /locus_tag="IVY85_001685"
     CDS             20613..>20790
                     /locus_tag="IVY85_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP8044704.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRI"
BASE COUNT         6662 a         3443 c         4279 g         6406 t
ORIGIN      
        1 catatgcttg tttcttttag tatggttata agattttttc tgacaatttt attatcactg
       61 atattaacac ctgtattcaa aatcatttca gtacaaacag gtatggtcga caaaccaaat
      121 gaaagaagaa taaataaagt tccaatgcct tcagcgggcg gactgcctat ctttatttca
      181 tttgttatat cgactttatt cttattccgt aaaattatcc ctcaatcgta tattgtaccg
      241 atcttgattg cttctttagt aatcatagtc acaggagtgc tcgatgataa atatgagctg
      301 acacctaaac aaaaaagtat agggatcttg attgcttctc ttattattta ttttgtagca
      361 gatatacgta ttgattcctt cacgcttcct tttattggat atatccaact aggctggctt
      421 agttttcccg taacgatttt ttggatcttc ggtatcacga atgcggtcaa tctgattgac
      481 ggtttggatg ggctggcggc agggatttca ttgatcggtt taattacgat aggaatcatt
      541 ggatattttt tcctccatgc ttctacagtg tatattccag ttgttatttt ctgtcttgta
      601 gcaagtatta tcggtttttt tccttacaat ttttatccgg caaagattta tttaggcgat
      661 acaggagctc tattcctagg gtttatgatg gccgtccttt cactacaagg gctgaagaac
      721 gtgacttttg tttcatcgat ttctttgctg atcgtcatgg gagtgccggt aacagacacg
      781 ttttttgcta tcattaggag aaaagcaaac cgtgtttctt tttctacagc agataaaaaa
      841 catcttcatc accgactaat cagtttaggc tttactcata aaggtgctgt cctgacgatt
      901 tatgcgatgg ctttgatgtt ttctttcaca gctatggtca tgaattatac tggaagaatc
      961 ggaacgatca tattgattat tgccatgttg tttgctgcaa ttttattatt tgaattgatt
     1021 ggtctgatca atgagaaaca tcagtttatt ttaaaaacat tgcgattttt agggaataaa
     1081 gagtaccgta cgcaagtaaa acagaaatat ttgaagaaat ggaaccgtca tatagatgaa
     1141 taacaaatta aaccagccga tccttattag tattgtgacg gcaaacagtg ataaaatttt
     1201 tcggacacta gataatttta tttcaactgt aaaagatgaa acagcagtaa aaatccgaat
     1261 ttttgacaac aattcacttc ccgagtacaa aaaaagacta acagagtata ggaaatatcc
     1321 ctacatcgat atctattttc atgatgaaaa ttccggattt ggttatggac ataatcacaa
     1381 tctgatagct agcgattctc gctatggcat tatttgtaac ccagatattt tagtggatga
     1441 agagacagtt acagcattgg tcttgttatt gaaagaacat ccggaatgtg cgatgttagc
     1501 acctaagatt cttaatgaag atggaacaac tcagcacctc atccgtgagc agctggctgt
     1561 ttttgattac gttttgcgct ttgtgccttt caaatgggtc aaaaaaaagt ttgacaaacg
     1621 actggctgtg tttgaatgcc gaggattgcc tactgatgct aatagttatg tacggatgat
     1681 ctcaggtagt tttatggtag ctgatattga taaatttaaa gagataaagg ggtttgatga
     1741 gcgtttcttc atgtactttg aagacaacga cttgtgtttg agttttgaaa aagcaggtta
     1801 caagttattg tacacaccct tttatgcagt tatccatttt tacgggaagg gtgcgcatcg
     1861 aaatgcaaaa ctgtttcgcg tgtttttgac ttctatgatg aaatttttca ataagtgggg
     1921 gtggcgtttt ttctgatgaa accagaaata ttagtagtcg ttgtgcttta tcagcaacta
     1981 ttcagtcaat ctccagctta tggaccatta agcaaagcac ttagcgaaaa gcaaattcag
     2041 cttattattt acgataatag tcctcaaagg cagcaaaatg agctgtttga aaaagaaaat
     2101 atcgtttatc atcatgatcc gcaaaatcct ggtttagcgg tggcttataa ttttgcgttg
     2161 tctcaagcag atgaaggaac acgttgtctc gtcactctgg atcaggatac gaggctagtc
     2221 gacgattatt ttgaactatt gcgtaaagta acctttacag atgaatgtgt tgctgcagtt
     2281 ccgatgattt tttctgggag ccgacaagtt tctcccctat attcagatcg ttatatcaat
     2341 cgtacagctc aagcagtaga agttgaaggc ccaacagctg aacgaatcat ggcaatcaat
     2401 tcaggtgttg cctggtctgt caaatttcta aaaaagattg gcgggtttaa tcctgctttt
     2461 tctttagatt ttttggatca ttggctgttt tggaaagtgg atcagttgga aaaaactgtg
     2521 gagatccttc ctgtacgaat ggaacatgat ctttcggttt tagactatga aaaagtttca
     2581 agtaatcgat accgttctat tttacaagca gaaaatcgat tttatcatga atatgatcaa
     2641 aggcatttat ccagtcaccg acgacaatta ttgttaagaa cagctaaaca gttcttgacc
     2701 gtcaaaaacc ggcatatctg gcggatgact ttgcgctcat atgttaagaa ttggaaggtg
     2761 tgaattcgtg cgttcggttt gtatagctac ctttaatgga ggcagatatt taagagagca
     2821 gttagatagt attttatctc agctttcagc agaagatgaa gtagtcattt ctgatgatgg
     2881 gtcagtagat gatacatggg atatattaac tgaatacgca gaaaaagata agcgaatcca
     2941 gcttttttcg gggcctcgtc aaggattgat cgctaatttt ggttatgcga tccagcagac
     3001 gaaaggagat ctaatctttt tggcagacca agatgatgtt tggttggaga caaaagtgaa
     3061 aaagatcact gactattttg agcagaatcc ggatcagcta gtagtagttt ctgatttaat
     3121 cattgtggac agcgagctgc aagagatcca tgattcgtat ttttcgtttc gaaattccaa
     3181 agaaggatgg cttaataatg tgataagaag tcactatatt ggtgctggta tggctttccg
     3241 gtcttcttta aaacccgata ttttgccgat cccgtcagaa gtgccgatgc atgatatgtg
     3301 gattggtttg ctgggaggaa agaagactgg gttcctaaga gaaccgttaa ctctgtaccg
     3361 aagacatgat ttaaacgcga gtgagattga aacacattct aaattacggc aaaaaatttc
     3421 ttggcgtgtt catctgatta cggcgttgat caaacgaaaa tggtttaaac gttgataaaa
     3481 gatgtttaaa tccttaatat ttccatgaca aatataacaa ttgatgagat aataacaaag
     3541 tgaaatttga aatcaaaaag gagagcagat atccatgaag ggaatcattc ttgctggggg
     3601 cagcggaaca cgtctgtacc ctttaacaaa ggcaacatcg aaacaattga tgccgattta
     3661 tgacaaacca atgatttatt atccaatgtc tactctgatg ttggcgggaa tcaaagaaat
     3721 tctgattatt tcaacgccac aagatacacc acgtttcaag gaattatttg gaaacggaaa
     3781 tgatttagga ctgcagatcg aatatgcagt acaagaaagt ccagatggat tggcacaagc
     3841 gtttatcatt ggagaagaat ttattggaga tgatagtgtc tgcttggtat taggggacaa
     3901 tatttactat ggtggcggtc tgtctaaaat gttgcaacga gcagcttcaa aagatacagg
     3961 cgcgacagta tttggctatc atgtcaatga tccagaacgc tttggtgtag tagaatttga
     4021 tgagaatatg caagctttgt cgattgaaga aaagcctgct cagccaaaat caaattacgc
     4081 tgtaactggc ctttattttt atgacaatga ggtaatttct atcgctaaag gtatcaaacc
     4141 ttcagaacgt ggagaactag agattacgga tgtcaataaa gcatatttgg ataaaggaaa
     4201 actatccgta gaacttatgg gacgcggatt tgcttggctt gatacaggaa cacatgaatc
     4261 attgcttgaa gcatctacct ttatcgaaac aattgaaaaa cgtcagaact taaaagtggc
     4321 ttgtttggaa gaaattgctt accgtatggg ctacatcgat aaggaacaat tgctggccct
     4381 tgcacagcca ttgaaaaaaa atcaatacgg tcaatattta ttgaatttgg ccgctgaata
     4441 aggagtcctt acagatgaaa gtaacagata caaaattaac agatgtcaaa atcgtagaaa
     4501 tggacgtctt tggcgatcat cgcggtttct tcacagaaag ttattcaaaa gcaaaatttg
     4561 cagaacatgg attgaaatat gactttgttc aagacaacca ttcactatca gctgaagctg
     4621 gtgtccttcg ggggcttcat ttccaaaaag gagaagctgc acagacaaaa ttgattcggg
     4681 tagtcactgg agcagtttta gatgtagtag tggatatccg caaaggtagc ccgacttatg
     4741 gtcaatggga cggatttatc ttatctgaac ataatcatcg acagttgctt gtacctaaag
     4801 gatatgccca tggattcgtt actttgacac caaatgtgaa cttcctttat aagtgtgata
     4861 attactataa tgcagaagca gatgggggca tcgcatttga tgatccagat ttagcgattg
     4921 attggccaat tgatccatca agagcgatta tgtcggaaaa agatcaccgt catccgacat
     4981 taaaagaatt tgaagcagaa aatccatttg tttacggaga aatctaagaa ttaaggagaa
     5041 attttcttat gaaaaacatc attgttaccg gaggtgccgg attcatcggc tctaatttcg
     5101 ttcattacgt ggtaaacaat catccagaag tgcatgtcac tgttttggat aaactgactt
     5161 atgcaggaaa caaagagaac ttggctggac ttcctagcga tcgcgtagaa ttggtcgtag
     5221 gagatgttgc agatgcagaa ttagtggacc gtctagtaaa agaagcggac gcagttgttc
     5281 attacgcggc agaatcccat aacgataatt cattaaaaga tcctttccca tttgtacaaa
     5341 cgaatatcat tggtacgtat acacttttag aagcttgccg caagtatgat gtacgctatc
     5401 atcacgtatc tactgacgag gtttacggcg atcttccatt acgtgaagac ctgccaggac
     5461 atggggaagg cgaaggagaa aaattcactg cggaaacacc atataatcct tcaagtccat
     5521 attcttctac aaaagctggt tcagatctgt tagtaaaagc atgggtgcgt tcttttggct
     5581 tacgtgcaac catttcaaac tgttcaaaca actatggtcc ttatcagcat attgaaaaat
     5641 ttattcctcg ccaagtaacg aatatcttaa gcggtattcg tccaaaatta tatggcgaag
     5701 gaaagaatgt tcgtgactgg atccatacga atgatcattc ttcagcagtg tggctgattt
     5761 tgacgaaagg cagaattggc gaaacctatc tgatcggtgc agacggtgaa gaagataata
     5821 agaccgtgat ggaattgatc ttagaaatga tgggtcaacc aaaagatgcg tacgaccatg
     5881 taaacgaccg tgcaggacac gacttgcgtt atgcaatcga ttcgacaaaa ttacgggaag
     5941 agttaggatg gaagcctgag tttacgaact tccgtgatgg gttggctgac acgatcaaat
     6001 ggtacgaaga acatgaagac tggtggaaaa aagaaaaaga ggccgttgaa gcagcatatg
     6061 ccaaaaatgg acaataatac agagaaaatg tcactgtatt aatggaaaaa cacatgtgag
     6121 gcaggcgctc gcctgtctct tttttaaaat aaaaatcaag gagcggataa tcatatgttt
     6181 ttattaactg gaggaaatgg gcaactaggt acagaattgc gccatttatt agatgaaaaa
     6241 aatctgcaat atgtttcaac agatgcaaaa gacttggata tcacagatgc cgagaaaaca
     6301 atggcttaca tcacagaatt aaaacctgat gttattttcc attgtgcagc ttacacagca
     6361 gtagataaag cagaagaaga agcaaaagaa ttagatgaga agatcaatgt tgatggtaca
     6421 agaaatgtag cacttgcagc aaaagctgtg aatgctcaat tcgtctatat cagtacagac
     6481 tatgttttcg atggaaaaaa caaaaatgta gaatacgaag tagacgacca aacgaatcca
     6541 ctaaatgaat atggtcgtac gaaattatta ggcgaaaaag cagtagaaga gattttagaa
     6601 gactattata tcatacgaac ttcttgggtt ttcgggatct atggacacaa tttcgtcttc
     6661 acgatgcaac gcctagcgga aacccgcgac aaactgactg tagtaaatga ccagtttggg
     6721 cgaccaactt ggacacgtac tttggcagaa tttatggtat atgtcatcga agaaaaagca
     6781 ccatttggta tttatcattt gtcgaacgaa aatagctgtt cttggtatga attcgcaaaa
     6841 gaaatattga aagatacaga tgtagaagtt gcacctgtta cgtcagaaga atttccgcaa
     6901 aaagcaacac gtccgcagta ttcagtcatg agcctgaaaa agacagaaga gttaggattt
     6961 gtcataccaa cttggcaaga agcacttgcg caaatgctgg aagctgccaa aaaaatgaat
     7021 aagtaatttc cttttatcaa atagaatttg gagcatgata tggaaaaaca aacaagaagt
     7081 gcacgacgaa aagaaaagcc aaaagcgaac caggcagatt cgaatttggt gcttgacctg
     7141 tttctaaaac tccgctatct tattggcgtt ttagtcatcg tggtcatcgt tacttttaat
     7201 ttaaacggaa gttcacttgg tgtttgggat aaatatgtgt cgcaaagaga cgatgggaaa
     7261 aaaactgatg tgatttttgg acagaatcgg gaagtgcggt cagacgaatg gctggtgcag
     7321 acaccttttt acctctcaca agctgaagag gactttccgt tggtgaacga ggattattca
     7381 ttaaacggac aaaacatgat cattgcgtat aattctcctg taaaggatat tacggttatc
     7441 ggaaaaccat tcaactgggg attctttttc ctcggaagag atcgaggact ttccttttat
     7501 tgggcgatga aattagtcgt catggtcttg cttggattcg aagtactgat gatattaacg
     7561 aagagaaaaa aagctctatc gctaatcggt gcatttgcgc tgacattttc tccagcagtc
     7621 cagtggtggt tcatgcaaca tgtgggcgat ttgattttct ttactttggg actgatgact
     7681 gctttttatc attatttcta caatcatgat aaaaaatggc tacgaacgtt gatgatgctt
     7741 ttggtggtta ttttcggtat tgggtttatt ttagtgattt acccagccca tcaagtgatg
     7801 ctggcgtact tgctgatttt ttattttgtt ggcctgctta tttactatag caagaaagtt
     7861 tcatgggatc tgttggatgt agtgttgatt ggaggggcag ttttatttat tggcggagtc
     7921 atggttcatt tctggctaac gtcaaaagac gcattgttag cttctttgaa taccttatat
     7981 ccaggaaaac gggtaagtac tggaggaaag tggacgattg gcgaattttt ttctttcctt
     8041 acaaattgga aaattccttt tgaagatatc aactattcga ataactctga agtggccctg
     8101 ttctatcact tttttccaac tgtattccta gcaagtccat ttgtgttatt tggcaaaaaa
     8161 gatagcgaac aaaagttact gggacgtatt ttgatgattt tttgtctgtt ttctatcttt
     8221 tggattaccg ttgggttgcc aaaatggcta gcagaaatca ctctgctgtc ttatgttcct
     8281 ccattaagag ccatccttac ttttagtttt gcggcttgtt tattatcgat ctggtttatc
     8341 gcttatattt ggagagaaga tgttattcct atttggtata aattcatttt gttggcagta
     8401 aatgcatggt cttatttcta tgcgattacg cattcgaaaa tggataatta tttttcggat
     8461 tttgaaacca tcgcagttgc tgtgttggga acagtgattt tagcttttgc attatttggc
     8521 tggagacgct tgttttacct cgcttttgcc gccttagtta tcgtatctgg ctttttcgtc
     8581 aatccagtcg tagaaggtac aggagcgatt tttgaaaaaa cactggcaaa agaaatccaa
     8641 aaaatcgatc aacaagatcc tggtaaagtt tggttgtctg aagatgaatt atataacttc
     8701 accccaacat tgggagtgaa atccttcaat acagtacgct tttatccaga tatagctgca
     8761 tggaagaaaa tcgatccgaa agaagaatac gagaagtatt ataatcggta tgctcataca
     8821 agagctttta tcgctaagga agatacaaag ttcattttag atcgtccaga tatgttttcg
     8881 gtcacgttga attttgaaga cgcagaaaaa ttagggatcg actatgtgat cagtaaacga
     8941 ccattaactg attacaacca attatatgaa gcgcagttca cgcagctata tggaccggat
     9001 aaagacggtt atatgatttt taaagtagaa tatccggatt atccagatgt accacaaaca
     9061 caaactcagg aaaatcagct agtcgtacag taataggcta gctgagaata atcctgaaat
     9121 gttagtagaa aggagagtgg agataattca agcaatcgta caaaaatttg tcaacaaaat
     9181 gagtgtcggt ttcttgcttt tgttactgac gctagtcttt tttgtttgga cagcagggtt
     9241 aggttttcca gcaactttgt ttatatataa caatagttca ttacttatcg cttttagtgt
     9301 ggctattttg ctcattttaa atgtttataa agcaaaaggc gtcgattttc tttatctagg
     9361 tgttgcgctt gcccttttca ttttcttttc ttactttcgg gaattaagaa atgagtcttt
     9421 atttggagac ttactgtttc ctttgattat tttattttgt ttagtaatta aatggatcga
     9481 gtggacaaaa tgggatcgcg gtatttatct gattgtcttt tcggtcttcc tcggaatcac
     9541 gttatatcgt gtatttacag agatccaagt accagaagga caaagcatct gggctccagg
     9601 caattcgctt tctgatattt ggatcaatac gaatataatc ggaagttctt tgatgactct
     9661 tgctttattg atcagtggat ttgccagttc ttttgaacga tggtatgtac gaatcatcgg
     9721 tgcagtagca gttgttgcag cttttctcgg gatttgggtc tgccaatcta gaggtgcatt
     9781 agtcgcgttg attgtttttg cgatattgga tcttttacct aaatcgttga tgcgtgtcat
     9841 tcgtgcgcca tttattgctt acacggttac gattctttta gctttgccga tttcttatct
     9901 ggcggctgtc tctgaaaaag tcaatttgtt taccggtcga gaagatattt ggcataaatt
     9961 ctatcagacg ctaggagaaa aatctgaaca aattttacta ggaatgaaaa catttatctt
    10021 ccaacgagga aaccaatttt taggaaacca caatagttat aattctattt tgaatatcta
    10081 tggattgata ggatttggga ttgctgcttt gctgcttatc ctttttattg gtcgcttaac
    10141 gttgaaagca gatttatcca acgggcagat gacttttatc tgggcctttt ttgcggtgat
    10201 gatgcagtca tttatggagg atacattgac ttcataccca tgggttccga ttatttatat
    10261 cctgttggct atggccgttc atcgatatga tgaaccagaa gttgcggagg aattctatgt
    10321 tatggaagag gcatatgacg atatgactta tgatgataca tcttatgagg aacaaccaga
    10381 tagcgctcat tcttccagga tccaacgcta tcattgatag gcacaaaata cgagagaggc
    10441 tgtgacaacc gtcgcagcct tttttggttt attgcaattg tctagcatct tctgtaaaat
    10501 agaaacgtta gagaaaatct tgtatcatag aaaattaaga aagaaataag aacgcagctc
    10561 ggtaccaaaa atatggtaaa atggcacttg actgtaattc actgaaaatc gcttggagga
    10621 acacaaatgt ttaaagattt gtatttattt atgaaggaca tttatcagaa cagaaaatta
    10681 ttgctacagt tttcgatcaa tgacttcaaa tcaagatatg caggatcgtt tttaggaatc
    10741 ttatgggcgt ttatcaatcc gctatttact gtattgattt attggctggt atttggtgtt
    10801 ggactgaaag cacaaatgat cgatgcaaaa tacccattta tcgtatattt agttaccggg
    10861 atgattccct ggttcttctt ctcagatgca tttgcatcaa ctacggttgt ttttcgggaa
    10921 tatacttatt tagtaaagaa agtcgtattc aatattcgta tcttgcccac gacgaaaatc
    10981 ctatccaatc tgtacaccca cttattcttc attctgattg gcatagctgt cgcgcttata
    11041 aatggtatat atccttcagc aatgtcttta cagctgattt attatttatt ttgcatgtgt
    11101 gctttcttga caggattgac ttggctgacg gcatcgatcc aaccattttt acctgatatc
    11161 atgcaattaa ttacgatcgt gctgcagttg atcatgtgga cattaccgat attatggagc
    11221 cctaatcagt ttgattcagg tcttgtaagc atattgaaat taaatccgct gtactatgtt
    11281 gttcaaggtt atcgtgagtc atttttaagt gaagcttggt tctgggagca tggaacatat
    11341 actttgtatt tctgggtttt cacgttagta atgttcgtaa tcggctctgc agtgttccgt
    11401 agattgaaac cacatttttc agatgtgctg taaggagaat agctatgaca gaatatgcaa
    11461 ttagactaaa agatatcaca aaatcttata atatgtacgc aaaaccttcg gatcgatttc
    11521 gtgaagccct caatcctttt aagaaatcct atcatgatgt attttatgcg ttgaaaaacg
    11581 tgaatgtaga aataaaaaaa ggtgagatga tcggtttcgt gggagaaaac ggatcgggaa
    11641 aatctacaat gttgaaaatc attacgggag tactgacacc aacatccggc acgatggaaa
    11701 tcgaaggaaa aatctcagct ttgctagaac taggatcagg attcaatcct gagtattctg
    11761 gctatgaaaa tatctattta aatggaatgg ttctaggatt ttcaagagaa gaagtcgatg
    11821 aaatggtcga tgatatcatt gaatttgctg acatcggaga ccatatttat caacctgtaa
    11881 aaacttattc aaacgggatg ttcgttcgtc tagcttttgc tgtagcaatc aatgtgaatc
    11941 ccgatatttt gattgtggat gaagcattgg cagtaggcga tcttgaattt caactaaaat
    12001 gtatggaaaa atttacagaa atcaagaact cgggaaagac gattttattc gtttctcatg
    12061 atatcagttc gattcgtcgt ttttgtgatc gttcctattg gttgaaaaac ggagaagtcg
    12121 tcgaatatgg cgatacgatg gaagtaacga cgaattatga aaacttttta aagaaaaaat
    12181 cagttaaaac agtggatcgg aataaaaacc aagtagaacg gttcgccgct ccagatatcg
    12241 tcgacgtgac gaaagcggaa ttgttggata aagacaaaaa tccggtggat atgctggaac
    12301 aaggagaaaa actatacgtt aaagtaactt atgaagtaaa agatgataca atcaaaaatc
    12361 cggttttagg aattgcgcta cgaacagtcg acaattttta tgtatgcgga cttaatactc
    12421 tactagatgg tatcaagatt ccttggaaaa aaggagagaa tatcttttat ctggagtatg
    12481 aaaagttagg tttgttagct ggtgaatatt attttgacgt tgcggtcttt gaagaaaatg
    12541 caacggttcc tttggtatat aagacaaagt acatgaactt gtttgtcagc ggttcttata
    12601 tcggagaagg catcgtggtc ttagaccata aatgggaaga agggactcat agcaatgaaa
    12661 tatgattttg aaatggacct agatgaacaa agcagtgtgg gaaaaattgc cgcacagatc
    12721 aaaccaggaa gtaaagtttt agagtttggt cccggaaacg gccgattaac gaaacattta
    12781 atcggagcaa aacaatgtga agtgagcatt gttgagttag ataaggaatt gttcgatttt
    12841 gtcagcgaat ttgctcaaga tggattttat ggtgatatcg aaagttttga gtgggcaaac
    12901 tattatgctg gacagacatt tgattacgtc ttgtttgcgg atgttttaga acatttggta
    12961 gatcctggaa aaacattgaa gaaagtcaga gaatttttga atgaagaagg agagatcctg
    13021 attacattcc ctaacttggc gcataattct gtcatgatcg atttgttcaa taaccaattg
    13081 ccgtgggctt cttatggtct cctagatgaa acccataatt ctttttatac tcatgatggc
    13141 ttccaaaaag tttttgaaaa agcgggatta ttcattaata ttgaagatta tttgtactta
    13201 gctgtaggtg atacagaatt aaaatcaact tatgaagaat tgccggaagc tgtacgctat
    13261 gatttcaaaa tgcgaccatt tggtgaagtc taccaatact ttttctcatt gatgaaacat
    13321 ccagtagcac aaagttccat tgcagagcca caaaattcta attatgtaaa agtgttagaa
    13381 gtaacccagc aaacgaaaca agatgaaaca atccaacaaa taccattcaa taatttcaca
    13441 ggggaaaatg agacactaag tttccccgta tcagaaacaa cagaaagaat ggtgtttcgc
    13501 ttcgcgcaac aacctagctt tatcgaattc agcgcagaag ctgccggaga aaaactaaca
    13561 tttatcgatt cgaatgctgt agtcaaaaca gtaaatgatt gttatttgtt tgatgggaaa
    13621 gaattaccag agttcgttct aacggatatt tctgggaaag aagtgactat ccattgtcac
    13681 tatcgcttta tcggggaatt gacaccaaca atgaaagaat tgttggaaac gattcgtccc
    13741 atggctgaag tcacaaagca gctttctgag aaaaatgacg aattagaaag agaaaatcat
    13801 catcttcttg aggaaaatac aagactagac catacgttaa aaacaaccac aaatcgctat
    13861 tgcacattgt tggaaagtga tgaatggaca ataaaacata gactaggacg acgtaagaaa
    13921 gaaacttcga aaaaaatcca agaaaaagaa ctttctatat gtattgacga gaaaatctgg
    13981 gatgcagaaa caaaaatatt aaaaatcatc ggatggggaa tcgctaattc ggatcgccag
    14041 cccttgtcct ataagttatc agctgaccaa tcgccatttt tcgaagcaat tcctgtttca
    14101 cgagaagaag tcaatcaagc agaacagctc gcaaaaggaa cagaagcggg atttgagtta
    14161 cgaatccttt gcgagcaaga acgttcattc ttagtggaag cagtagctca aaatggccaa
    14221 tcatggatca ttgaaatgta aataatgatt aacttataaa gataaggagt aaacgaatcg
    14281 tgcaaaaaaa agaaattgcc gtatttatcg atcaaatcac tcgtgaacgg gccactggcg
    14341 atttgcttat tgtgggctgg gcaattgatg aagtgaccaa agaaatccct acgataaagg
    14401 tagaaaaaga aaatgtgatt gctgaagcaa cacatgtcgt aagattggat atcaatcatt
    14461 tatataatct tgatgttaag acccaaagcg gttttaaggt acgtttatca gggaaaatga
    14521 gaggaaaagc catcttagac ttccaaacag cgagacatca aaatggcata gctgtgaaat
    14581 taaacggtaa atacccttat gatgacggca ttgaatcttc atgggaaaga aaaaaaagat
    14641 tactgaaaaa agggataaat tatgcaagga ctcatggcgt aaaaaaagcg atccgtcggg
    14701 tcaaattgga attgaagcca ggatcgatcg actatgcaga gtggatcagt cgtcatgaga
    14761 aaccagattt gaaagagcaa aggaaagaag cacaaggatt tgcgtaccgt ccattgattt
    14821 ccatacttat gcctgtctat aacgttgaaa tcaaatggct cgaaaaatgc attgattcgg
    14881 tactggatca aacgtatgat cattgggaac tatgtatcag tgatgatgct tctactgatc
    14941 cagctatccg aaagtgtctg gaatcctatc aagcaaaaga tgatagaatc aaagtcgttt
    15001 tccgaaaaga aaatggacac atcagtctgg caactaattc tgcattggaa atagcagaag
    15061 gagaatttat agcactctta gataatgatg atgaattacc gccatttgca ttatatgaag
    15121 tggcaaaagt gctaaacgta catccagaac tggatgtgat ctacagtgac gaagataaaa
    15181 tcgatgcaga tggcaatcgc tttgatccgc atttcaaagc agactggtcg cctgatacat
    15241 tgatgggaaa taattatatc tctcacttag gcgtatatcg aacaagcatt gttaaagaac
    15301 ttggcggttt ccgtaaaggc tatgaaggtt ctcaagatta tgatttggtc cttcgtgtga
    15361 cagaacaaat ccctgcagat catatttacc acattgaccg agtcttgtat cattggcgga
    15421 cgatcccagg atcaactgca agtaatggcg aagccaaaag ttatatctat gattccggag
    15481 tgaaagcatt gacagatgcc ctgtctagaa gaaacatcaa aggaagcgta cgtcctggca
    15541 gaatttctgg attttatgag attgcttatg acgtcttgca agaggatttg gtcagtgtga
    15601 tcattccaac gaagaatggc tatgaagatc taaaaacttg tgtggattcg atcatcgaaa
    15661 aaacgtctta ttcaaattat gaaatcatta ttgcggataa cggaagtaca gatccaaaaa
    15721 tgcaagaatt atttgcagaa tacaagcatc agctgaaaga ccgctttatc gtagaattga
    15781 ttgacattcc attcaattat tctcgaatta acaatttagc agcagaaaaa gcaaacggca
    15841 aatacttttt attcttgaat aacgatacag aagtgattga gccagattgg atgacggtga
    15901 tggtttctta tgcacagttt gatcgaatcg gctgtgtagg agcaaaactg ttttatccag
    15961 atgatacaac gcagcatgca ggagtattat taggaatcgg tggtgttgca gggcatgcat
    16021 tgaataacta tgatcgaaca cattgtggtt atttcggccg attggtcatt gacgtcaatt
    16081 acttagcagt cactgcagct tgtatgatgg tcaaggcagc tgacttcaat gctgttaacg
    16141 ggtttgatga aacattagaa gtagcattca acgatgttga cttgtgcttg aaagtttatg
    16201 aactgggtcg ttacaatgtc tatgcacatc aagcagaatt gtaccatttc gaatctaaat
    16261 caagaggtta tgaagatact ccagaaaaac aaagacgttt tgctggtgaa atcaaaaaaa
    16321 tgcaagataa atggccagca tatattgcgc atgatccatt ttacaatgat aatttaacaa
    16381 aagaaggaat cggcgacttt tcgttgagac cagattaaac tataataaaa agctgttatg
    16441 ttgtgattcg ctcgcacata acggcttttt atagtttttt gtcaaaaaaa ggcagaaggt
    16501 gccgactcaa aaaaataagt cgaggttggg acagaagtgt ttaactccga gaaataagaa
    16561 gaaattcacg aaaattgctt ttcaaatttt tgtgaatttc ggcttattta agaagaagtt
    16621 gcttctgctc ccaccgttta ttcgttttta aagaatgaga caaaagtgtt tcttactttt
    16681 gtcccacgct cttattcatg ctcagagcgc caatagatac aaaatcatag ccaaagtttc
    16741 acgaacccta gctgtcaaag aatcgatatt ttattttaat ataggctatt ttaaaataaa
    16801 tggtttaagg tattcctcta caaaagccac tctatcaatt atcatgggtt taaaataaga
    16861 agtgatggag ataaccagat tgttcttagc gtcaatataa agaacatttc cactattacc
    16921 aattgctgaa tatatttcgt tctcagaatc taatacccac cataaatagc catatctcat
    16981 attttgtaag tcttctaaag cactggttct aggtttgaac atctcgttta tccacatggg
    17041 tggtacgatt tgttttcctt tatacatccc tttatttagg cataatacac caatttttgc
    17101 cagatcactt gccgataaac acaaaccata tcctgccgtc ccgatattta gtggatcaca
    17161 aaaccagaca ttggttttag gagacttatc cattgtaaac tgcttatgtt cttcagctgt
    17221 ttcagcataa taattgacat ggtttggtat ccctatagga tcaaatagat acttattggc
    17281 aaaatcaaca gttgttagct tacttgtctg atacaacaag ccagttaata tgtgtaaaca
    17341 gacagtttga tagttgaatt cgccggttat accttttctt cctcccaata aatctagtga
    17401 tgtttttgtc caatcttcac ttgaacagac ttttgtccat ggatcgcctt tacatttgta
    17461 gggtgctttc attgttagca agtgcttgat tgtaatagcg taaatagttt tctccccgcg
    17521 tttcacttta tactctggaa aataatctaa tactttatca tcgatacttt tgattaatcc
    17581 ttgctctaaa gcaatgccaa tcagcaaaga tacaatactt ttcgttgctg acatgatatg
    17641 acaagtatcg tcttttttat aaccattcca ttcatctgta taaacttcta ccccatcctt
    17701 atatgcgaca atttgacata tatttggttg tcgaaaacca ataaaatcat gcaactcttg
    17761 tttattcata agatattacc tcactttctt ctataagcat aatgtcatta aaaatcgatt
    17821 tcaatttttt cgattcttca cgtaatacag gtattcttat ggactagaca gcgctttggg
    17881 aagatggatt ctctgaaaaa tataaaggtt cactttatga gtggataaaa aaagcagcaa
    17941 atacttcgac ttctttaatt taccaacatt agtgaaaatc gattcatctg gagatttgta
    18001 atttagagtc cttgtcttga ttagaggtgt ataaagcttt ttattagaca tttttaaatt
    18061 ccattacaat ttgcttataa actaggtttt taaaggtgac tatggtaaga taatagagat
    18121 attattttgt gattaggagt gtatatatga ataaaaatgg agaatggagt gctgcacgac
    18181 ggatattcat tatattaatg gatgttattg tatacaatgc agcaatatat agtagtttct
    18241 tactgaagtt tcgtggagag atcccattac gaaatttaga aactttccaa cactcagcaa
    18301 ttattatttc agtgattttt attgcgttga acattttgtt gggtgcatat gttttttata
    18361 atcgaatgat cagtgacatc gtgtttgtca cagtcatcgg tcaggtattg atgacactag
    18421 ggatcatggt agtgactttt gcgggccgct ggtttgcttt tccacgtctt gttatcttac
    18481 tttcatttgt tctcggtacg cttttcttaa gtatctatcg tattctcgta tataaacttt
    18541 atttacgcgt aagtcgagat aaaaaggtcg tcattattgg gttagaaaag gatgtcgcac
    18601 cagcaatcca aaattttggt tcaaagaaaa acaacaaaca taaagtcgaa gcagtcattc
    18661 ttgataacta ttatgcaaac atcaaaaata tgattaaaga catcgatatt gtctatcttg
    18721 cttctcatat cgaagaaact gaaaaaatca aaatctatca attagttact aaaaatgaaa
    18781 aaaaattgtt tttgaataca acatttgaaa acttgacatt gatcaatcca aatattatga
    18841 atttcgaaga tgaaagtatt attgaagcat ctggatttag aatccaacct gaatatgaac
    18901 tattcaaacg tttgtttgat atcatcgttt cacttgtttt attagttatt gcttcaccat
    18961 ttatgttatt cacagcaatt ttagtcaaag tgacatcacc gggaccaatc atctataaac
    19021 aagtgcggat cacaaaaaat caaaaggaat tttcaatcta taaattccgc tcgatgaccg
    19081 ccacagccga agcaaaatct ggaccagttt tagctaagag caatgatgca cgagtaacgc
    19141 ctgtaggtaa atttattcga gctgtccgct ttgatgaatt accacaaatc ttcaatgtgc
    19201 tgaaaggcga tatgtcgatc gtaggaccac gtcctgaacg tcctttcttc gtcgatcagt
    19261 tcaatgcaga aaacccttat tactacttac ggcataatgt tcgtgctggt atcactgggt
    19321 atgcacaagt ctatggaaaa tatgtctctg attacaacag taaattacgt tttgatttac
    19381 tttatatcaa aaaatattca ttgatgcttg atttaaagat cttgctgcaa accatcaaga
    19441 tcctatttga taaaatgtcc tctcaaggac ttgaagaaga cgaaaatgtg atctcattgg
    19501 cagaaattgc ttttccgaat gaaaaaatct ttcgctagtt gggaggcgta aaaatgaaca
    19561 atccgaaatt cagcataatc gtaccagtat acaatgcaga agcaactatt ggtcgaacga
    19621 ttgatattct aaaagaattg gattatgaag agtacgaagt cgttttagta aatgatggat
    19681 caacagatca aacacaggaa atcattcaag aactgattga aaatgatgaa cgttttgaat
    19741 tgattactac gaccaatcaa gggcccggat tagcaagaaa cgaaggaata agacagtcaa
    19801 aaggagaata tctcttattc ttcgatgcag atgacacgcc agtaaaaacg atcttgtcag
    19861 attatgatca tttgctagaa aagagtccaa aagcggatct tgttgttagt tcgtttgttt
    19921 ttcgtacaat ggacgaggga agaatcgttt ccgaaaaaga aaacttagtt acagaatgtc
    19981 actatctaac tcacgaagag tttataaaag acatgtatca gttaatgaat gaacagttga
    20041 tgtatgttgt atggaacaaa tgctatcgta gcgatattct gaaaaataac caaattctat
    20101 ttaaaactta taatagttgt gaggatcgaa tatttaactt acattattat gaatactgtc
    20161 atcaagtgat catgaaccca aaagtagaat atatttatga atttgaaggt gggaagggga
    20221 tcactaatca ataccgacct aacaagttcg aaacatttaa agaattctat tcattagcaa
    20281 atactgtaac agacgatatt aataaagacg gtatggcggc gcttctttta aaagggacaa
    20341 cttcagttat tttttctatt tatagtacct ctgcaagaac ttcaaaagag aagaaagacg
    20401 aagcaaagca gatattgagt gatccagtga tccgagaagc caagcaaatt gctaagacgg
    20461 attcttcagc aaaaaaaata acaaagttgc tttacaatat gccagctcca ctatttttat
    20521 ctgctgtaaa agttggaagc ttcgttgaag tgaaaatgcc agggttaatg gctgctttga
    20581 agcgagtata ttagttaatg gaggaaaata taatgaaaag aattttgcat tttcagggac
    20641 gaatgggttt aggtggagca gagtctttca tgatgaattt atatcgtaaa attgatcgta
    20701 caaactatca atttgatttt ttgatttatg aagattatgc agatgttcaa gactatcatt
    20761 cagagattga aagattagga ggccgtattt
//
LOCUS       DACCYH010000048        20555 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.049, whole genome shotgun sequence.
ACCESSION   DACCYH010000048 DACCYH010000000
VERSION     DACCYH010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.049"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            300..608
                     /gene="rpsJ"
                     /locus_tag="IVY85_001686"
     CDS             300..608
                     /gene="rpsJ"
                     /locus_tag="IVY85_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP8044705.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            639..1268
                     /gene="rplC"
                     /locus_tag="IVY85_001687"
     CDS             639..1268
                     /gene="rplC"
                     /locus_tag="IVY85_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP8044706.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            1296..1919
                     /gene="rplD"
                     /locus_tag="IVY85_001688"
     CDS             1296..1919
                     /gene="rplD"
                     /locus_tag="IVY85_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP8044707.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            1919..2209
                     /gene="rplW"
                     /locus_tag="IVY85_001689"
     CDS             1919..2209
                     /gene="rplW"
                     /locus_tag="IVY85_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP8044708.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            2248..3081
                     /gene="rplB"
                     /locus_tag="IVY85_001690"
     CDS             2248..3081
                     /gene="rplB"
                     /locus_tag="IVY85_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP8044709.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            3124..3402
                     /gene="rpsS"
                     /locus_tag="IVY85_001691"
     CDS             3124..3402
                     /gene="rpsS"
                     /locus_tag="IVY85_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP8044710.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            3425..3772
                     /gene="rplV"
                     /locus_tag="IVY85_001692"
     CDS             3425..3772
                     /gene="rplV"
                     /locus_tag="IVY85_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP8044711.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            3786..4442
                     /gene="rpsC"
                     /locus_tag="IVY85_001693"
     CDS             3786..4442
                     /gene="rpsC"
                     /locus_tag="IVY85_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP8044712.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            4445..4879
                     /gene="rplP"
                     /locus_tag="IVY85_001694"
     CDS             4445..4879
                     /gene="rplP"
                     /locus_tag="IVY85_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP8044713.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            4869..5057
                     /gene="rpmC"
                     /locus_tag="IVY85_001695"
     CDS             4869..5057
                     /gene="rpmC"
                     /locus_tag="IVY85_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP8044714.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            5082..5348
                     /gene="rpsQ"
                     /locus_tag="IVY85_001696"
     CDS             5082..5348
                     /gene="rpsQ"
                     /locus_tag="IVY85_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP8044715.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            5406..5774
                     /gene="rplN"
                     /locus_tag="IVY85_001697"
     CDS             5406..5774
                     /gene="rplN"
                     /locus_tag="IVY85_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP8044716.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            5811..6119
                     /gene="rplX"
                     /locus_tag="IVY85_001698"
     CDS             5811..6119
                     /gene="rplX"
                     /locus_tag="IVY85_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP8044717.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            6147..6686
                     /gene="rplE"
                     /locus_tag="IVY85_001699"
     CDS             6147..6686
                     /gene="rplE"
                     /locus_tag="IVY85_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP8044718.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            6705..6890
                     /locus_tag="IVY85_001700"
     CDS             6705..6890
                     /locus_tag="IVY85_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP8044719.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            6926..7324
                     /gene="rpsH"
                     /locus_tag="IVY85_001701"
     CDS             6926..7324
                     /gene="rpsH"
                     /locus_tag="IVY85_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP8044720.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            7356..7892
                     /gene="rplF"
                     /locus_tag="IVY85_001702"
     CDS             7356..7892
                     /gene="rplF"
                     /locus_tag="IVY85_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP8044721.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            8053..8409
                     /gene="rplR"
                     /locus_tag="IVY85_001703"
     CDS             8053..8409
                     /gene="rplR"
                     /locus_tag="IVY85_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP8044722.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            8430..8930
                     /gene="rpsE"
                     /locus_tag="IVY85_001704"
     CDS             8430..8930
                     /gene="rpsE"
                     /locus_tag="IVY85_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP8044723.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            8945..9124
                     /gene="rpmD"
                     /locus_tag="IVY85_001705"
     CDS             8945..9124
                     /gene="rpmD"
                     /locus_tag="IVY85_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP8044724.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            9168..9608
                     /gene="rplO"
                     /locus_tag="IVY85_001706"
     CDS             9168..9608
                     /gene="rplO"
                     /locus_tag="IVY85_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP8044725.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            9608..10903
                     /gene="secY"
                     /locus_tag="IVY85_001707"
     CDS             9608..10903
                     /gene="secY"
                     /locus_tag="IVY85_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP8044726.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            10965..11612
                     /locus_tag="IVY85_001708"
     CDS             10965..11612
                     /locus_tag="IVY85_001708"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP8044727.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            11803..12021
                     /gene="infA"
                     /locus_tag="IVY85_001709"
     CDS             11803..12021
                     /gene="infA"
                     /locus_tag="IVY85_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP8044728.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            12054..12170
                     /gene="rpmJ"
                     /locus_tag="IVY85_001710"
     CDS             12054..12170
                     /gene="rpmJ"
                     /locus_tag="IVY85_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP8044729.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            12188..12553
                     /gene="rpsM"
                     /locus_tag="IVY85_001711"
     CDS             12188..12553
                     /gene="rpsM"
                     /locus_tag="IVY85_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP8044730.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            12581..12970
                     /gene="rpsK"
                     /locus_tag="IVY85_001712"
     CDS             12581..12970
                     /gene="rpsK"
                     /locus_tag="IVY85_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP8044731.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            13050..13988
                     /locus_tag="IVY85_001713"
     CDS             13050..13988
                     /locus_tag="IVY85_001713"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP8044732.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            14016..14396
                     /gene="rplQ"
                     /locus_tag="IVY85_001714"
     CDS             14016..14396
                     /gene="rplQ"
                     /locus_tag="IVY85_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP8044733.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            14668..15507
                     /locus_tag="IVY85_001715"
     CDS             14668..15507
                     /locus_tag="IVY85_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8044734.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            15483..16352
                     /locus_tag="IVY85_001716"
     CDS             15483..16352
                     /locus_tag="IVY85_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8044735.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            16342..17139
                     /locus_tag="IVY85_001717"
     CDS             16342..17139
                     /locus_tag="IVY85_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8044736.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            17342..18088
                     /gene="truA"
                     /locus_tag="IVY85_001718"
     CDS             17342..18088
                     /gene="truA"
                     /locus_tag="IVY85_001718"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8044737.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            18753..18990
                     /locus_tag="IVY85_001719"
                     /pseudo
     CDS             18753..18990
                     /locus_tag="IVY85_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(19134..19910)
                     /locus_tag="IVY85_001720"
     CDS             complement(19134..19910)
                     /locus_tag="IVY85_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8044738.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            20033..20440
                     /locus_tag="IVY85_001721"
     CDS             20033..20440
                     /locus_tag="IVY85_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAP8044739.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLIRNYKISGGGC
                     YLLECRFTSKKSMNSFLEQLNEYANYKLSIVID"
BASE COUNT         6882 a         3658 c         4380 g         5635 t
ORIGIN      
        1 aaaagacagg agtattataa atagaagaaa cacgaaataa aagagtcagt attttctaaa
       61 tgaaacttta tgaaaacaaa atgtttttta caagaaaaag ccttgctttg tgagtttata
      121 tatattataa tgacaaaggt gcgtttactt tagtgaattg cacagacgta atcagaatga
      181 tttcgtcggc taagaaacga gaggttgcga cacgcccgga cgctttgcca cgaacgagcg
      241 tgacggaaat tttcgtggag ctatgtctac ttttcaaaat aggcgaagga gggaacaaga
      301 tggcaaaaca aaaaattcgt atccgtttaa aagcgtatga acaccgtatt ttagatcaat
      361 cagcggataa aatcgtggaa actgcaaaaa gaactggagc tagcgtgtct ggtccaattc
      421 cattaccaac tgaacgcagt ctttacacaa tcatccgtgc gactcataaa tacaaagatt
      481 cacgcgaaca attcgaaatg cgtacacaca aacgtcttat cgacattgtg aacccaacac
      541 caaaaactgt tgatgctttg atgaagctag acttaccatc tggtgtaaac atcgaaatca
      601 aattataaaa ctaaaacaaa aataatggag gtgtactcat gaccaaagga atcttaggga
      661 aaaaagtggg aatgacacaa atcttcactg aatctggtga attaattcca gtaactgttg
      721 tagaagctac tccaaacgta gtactacaag tgaaaacaat ggaaacggat ggctacgaag
      781 ctattcaagt tggttaccaa gatatgcgtg aagttttatc aaacaaacct gcgaaaggtc
      841 atgttgcaaa agcaaacacg gctcctaagc gcttcattcg tgaattcaaa aatgttgagc
      901 taggagaata tgaagtagga aaagaaatta cagtagatgt tttccaagca ggagacatta
      961 ttgatgtaac aggtactacg aaaggtaaag gattccaagg ggttatcaaa cgccacggac
     1021 aaagccgcgg accaatggct cacggttctc gttaccatcg tcgtcctggg tcaatgggtc
     1081 cagttgcacc taaccgtgta tttaaaaaca aacgtcttgc tggccgtatg ggtggcaacc
     1141 gcgtaactat ccaaaacttg gaagttgtac gtgtcgatgc agaaagaaat gttatcttga
     1201 tcaaaggtaa tgttcctgga gctaaaaaat ctttaattac tatcaaatca gctgtgaaag
     1261 ctaaataatt gtaggaagaa aggaggaact aaggaatgcc gaatgtagca ttattcaaac
     1321 aagatggaag ccaaaacggt gaaatcacac ttaacgaaga aatcttcgga attgaaccta
     1381 atgaaagtgt tgtttacgat gcaatcgtaa tgcaacgtgc gtcattaaga caaggaacac
     1441 acgcggttaa aaaccgtagc gctgttcgcg gcggcggacg taaaccatgg cgtcaaaaag
     1501 gaacaggtcg tgcacgtcaa ggatctatcc gctcaccaca atggcgtgga ggtggcgtag
     1561 tctttggacc aacaccacgt tcttacagct acaaacttcc taaaaaagtt cgtcgtttag
     1621 caatgaaatc tgtattgtca gaaaaagttg ctgaaaacaa cttggtagca atcgaaggct
     1681 tgaacttcga cgcaccaaaa acaaaagaat tcaaacaagt tcttgctaac ttatctatcg
     1741 actctaaagt attggttgtt ctagaaaaag gtaacgactt tgctgcttta tcagcacgta
     1801 acttaccaaa cgtttctgta gtaacttctg ataatgtgag tgtgctagac gttgtatcaa
     1861 atacaaaagt gttagcaaca caaactgctc ttactcaaat tgaggaggtg cttgcataat
     1921 gaacttacta gacgtaatca aacgcccagt gatcactgaa aaatcaatgc ttgccatgga
     1981 tgacaagaaa tacacttttg aagtggacac tcgcgcaaac aaaactttag taaaacaagc
     2041 ggtagaagct gcttttgacg ttaaagtcaa aaacgtgaac atcgtgaacg tacgtgcgaa
     2101 attcaaacgt atgggcaaat atgcaggata tacaaaaaaa cgtcgcaaag cgatcgttac
     2161 attgactgaa gattcaaaag aaatccaatt attcgaagct gctgaataag cactaataga
     2221 taactcatta aattaggagg gaaaaacgtg gcgattaaaa agtacaaacc tacctcaaat
     2281 ggtcgtcgta acatgacagg ttctgatttt gctgaaatca caacatcaac acctgaaaaa
     2341 tcattgttac aaccattgaa aaaccatgct ggacgtaaca acaacggccg catcactgta
     2401 cgtcatcaag gtggcggaca caaacgccaa tatcgcgtga tcgacttcaa acgtaacaaa
     2461 gataacgttg ttgcaactgt tcaaacgatc gaatatgatc caaaccgttc agctaacatc
     2521 gcgttagtac actatgaaga tggagtgaaa gcttacatct tagcacctaa aggactagaa
     2581 gtgggtatga aacttgtttc aggtccagaa gcagatatca aagtcggaaa tgctcttcca
     2641 ttagaaaaca ttccagttgg tactgttatc cacaacattg aaatgaaacc tggtaaaggt
     2701 ggacaattga tccgttctgc tggtacaagt gctcaagtac ttggtaaaga aggcaaatac
     2761 gtattgatcc gcttgaactc tggtgaagtt cgtatgatct tagctgcatg ccgtgctaca
     2821 atcggttctg taggtaacga acaacacgaa ttaatcaaca tcggtaaagc tggacgctct
     2881 cgttggatgc gtaaacgccc aactgtacgt ggtagcgtaa tgaaccctaa tgatcaccca
     2941 cacggtggtg gtgaaggtaa agctcctatc ggacgtaaag caccagtttc tccttggggt
     3001 caaccagctc ttggttacaa aactcgtaac aaaaaagcta aatcagacaa acttattgtt
     3061 cgtcgtcgca aaaataaata attactttga ccatgtgtcg cacattttga gaggaggttc
     3121 accatgggtc gtagtttaaa gaaaggacct tttgtcgatg agcatttaat gaaaaaagtt
     3181 gaagctcaag caggggttga aaagaaaaaa gttatcaaaa cttggtcacg ccgttcaaca
     3241 gttttcccaa gttttgttgg atataccatc gcagtttatg atggacgtaa acatgtacca
     3301 gtttacatcc aagaagacat ggtaggacac aaattaggtg aatttgcacc tacaagaact
     3361 tatcgtggac atgctgctga cgataagaaa acgaaacgct aatttgaggg gaggataaat
     3421 ccaaatggca gaacaaatca catcagctaa agcaactgca aaaacagttc gcacttcacc
     3481 tcgaaaagcc cgtttagtaa ttgaccttat cagaggtaaa agtgtcgcag atgcaatttc
     3541 aatcttgaaa ttcacaccaa acaaatctgc tggaatcatt gaaaaagtat tgatgtcagc
     3601 agttgcgaat gcagaaaata actttgactt agatgttgaa aacttggtag taagcgaagc
     3661 ttttgttaac gaaggaccta caatgaaacg tttccgtcct cgtgcaaaag gatcagcttc
     3721 tccaatcaac aaacgtacaa gtcatatcac ggtagtcgta tcagaaaaat aaggagggat
     3781 aatcagtggg tcaaaaagta catccaattg gaatgcgtgt aggcatcatc cgcgactggg
     3841 atgctaaatg gtatgctgaa aaagagtatg ctgagttctt acacgaagat ttaagaatcc
     3901 gtaaatttat ttcaactaaa cttgctgatg ctgctgtatc aacagttgaa atcgaacgcg
     3961 caacaggtcg cgtaaacatt tcaattcaca cagctaaacc aggtatggtt attggtaaag
     4021 gcggttctga agtcgaaagc ctaagaaaag aattaaacaa attaactggt aaaagaatcc
     4081 atatcaatat cgtggaaatc aaaaaaccag atctagatgc aaaattagta ggtgaaggaa
     4141 tcgcacgtca attagaaaac cgtgttgctt tccgtcgcgc tcaaaaacaa gccatccaac
     4201 gctcaatgcg tgctggcgct caagggatca aaactcaagt atctggtcgt ctaaacggag
     4261 cagatatcgc tcgttcagaa ggatactcag aaggaactgt tcctcttcat actttacgtg
     4321 cggacattga ttacgcatgg gaagaagcag atacaacata cggaaaacta ggagttaaag
     4381 tgtggatcta tcgtggagaa attcttccaa ctaaaaaaaa cactgagaaa ggagggaaat
     4441 aatcatgtta gtacctaaac gtgtaaaaca ccgtcgtgaa ttccgcggaa aaatgcgcgg
     4501 ggaagctaaa ggcggaaaag aagtagcatt cggtgaatac ggtttgcaag ctgttgattc
     4561 acattggatc acaaaccgcc aaatcgaagc tgctcgtatc gcaatgactc gttacatgaa
     4621 acgtggtggg aaagtatgga ttaaaatttt ccctcacaaa tcttatactg ccaaagcaat
     4681 tggggtacgt atgggttctg gtaaaggggc acctgaagga tgggttgcac cagtaaaacg
     4741 tggtaaaatc atgtttgaaa tcgcaggcgt tcctgaagaa gtagctcgtg aagcgttacg
     4801 tctagcttct cacaaattac caatgaaaac taagatcgta aaacgtgagg aaatgggtgg
     4861 tgaatcgaat gaaggttaaa gaaatcagag aattaaccac tgccgaaatg cttgatcaag
     4921 aaaaacaatt aaaagaagaa ttgtttaatc ttagattcca attagcaaca ggtcaattag
     4981 aaaacactgc acgtattaaa gaagtacgta aatcgattgc acgcatcaaa actgtgttgc
     5041 gtgaacaagc taagtaatta tggaaggagg ccattaagcg tatgactgaa ggaagaaatc
     5101 aacgcaaagt ttatcaaggt cgcgtggttt cagacaaaat ggataaaaca attaccgttg
     5161 ctgtcgaaac aaagaaaaac caccctatct atggcaaacg catgaactat tctaagaaat
     5221 acaaagtgca tgatgaaaac aacacagcaa aagttggcga catcgtgaga attatggaaa
     5281 ctcgtccatt atcagctaca aaacgtttcc gtttgttgga agtagtcgaa gaagcagtta
     5341 ttatctaata acacgagatt ttcctatata gattgaatgc aaaatctgaa aggaggatac
     5401 acaacgtgat ccaagcagaa agtcgtttaa aagtcgctga taactcaggc gcacgtgaaa
     5461 ttttgacgat caaagtcctt ggtggatctg gtcgtaaaac tgctaacatc ggtgatgtga
     5521 tcgttgctac ggttaaacaa gcaacgccag gtggggttgt caaaaaaggt gacgtcgtta
     5581 aagccgttat cgttcgaaca aaatcaggag ctcgtcgtac agacggttca tatattaaat
     5641 ttgatgaaaa tgctgcagtg attattcgtg atgataaaag ccctcgtgga acacgtatct
     5701 ttgggccagt tgcacgtgaa ctacgtgaaa acaacttcat gaagatcgtt tcactagcac
     5761 cagaagtttt ataatcttga gacacttatc aaaggaggtg cgaaacagtt atgtttgtta
     5821 aaaaaggcga taaagttaaa gttatcactg gaaaagacaa aaacaaagaa ggcgttgtac
     5881 tagcagcgtt cccgaaaaaa gataaagtta ttgtcgaagg tgtaaatgtc gttaaaaaac
     5941 accaaaaacc ttctcaagct gctcctcaag gtggaattgt tgagatggaa gcgccaattc
     6001 atgtttctaa cgtgatggtg gttgactcta ctggtgtagc tggccgagtt ggctacaaag
     6061 aagtagacgg caaaaaagtc cgtgtttcta aaaaaaccgg tgaagtttta gataaataaa
     6121 agtttaagga aggagggtct tactgaatga accgcctaaa agaaaaatat cttaaagaag
     6181 taactccatc attgatggaa aaatttaact atagctctgt tatgcaaaca cctaaagttg
     6241 aaaaaatcgt catcaacatg ggtgttggtg atgcggtatc aaacgctaaa aacttagata
     6301 aagcagttga agaattaact ttgatcactg gtcaaaaacc aatgattaca aaagctaaaa
     6361 aatcaatcgc tggattccgt ttacgtgaag gaatgccaat tggtgcaaaa gttacattgc
     6421 gcggagaaag aatgtacgaa ttcttagaca aattggtttc tgtttcacta ccacgtgtac
     6481 gtgacttcca tggtgtaagt aaaaaagcct ttgacggtcg tggaaactat actttaggta
     6541 ttaaagaaca attgatcttc ccagaagtag attatgattt agtagataaa gtacgtggta
     6601 tggacatcgt tattgtaaca acagcaaact ctgatgaaga atctcgtgag ttgttgacac
     6661 aattaggcat gccattccaa aaataattaa aggaggcgaa ctacgtggct aaaaaatcaa
     6721 tgattgctaa aaacaaacgt cctgcaaaat tttcaacaca agcttatact cgttgcgaac
     6781 gttgcggacg tccacattca gtttatcgta agttccatct ttgccgtatt tgcttccgcg
     6841 aacttgccta taaaggtcaa attcccggcg tgaagaaagc tagctggtaa aaagtaaatt
     6901 cgcataagaa ggaggtaaaa gatcaatggt catgacagat ccaattgcag attttctaac
     6961 tcgtatccgt aacgcaaaca tggttaaaca tgagtcttta gaagtacctg catcaaaaat
     7021 taaacgtgac atcgctgaaa tcttgaaacg tgaaggtttc gttcgtgatg ttgaatacat
     7081 cgaagatgac aaacaaggcg tgatccgtgt tttcctaaaa tttggtaaaa acggcgaacg
     7141 tgttatcaca aacttaaaac gtatctctaa accaggtttg cgtgcttatg tcaaagctga
     7201 cgaagtacca aaagtattga acggtttagg tatcgcaatc atttcgactt ctgaaggtgt
     7261 tgtcactgat aaagaagctc gtgaaaaaaa tatcggcggc gaagttatcg cttacgtatg
     7321 gtaatcaagt agaatctaca aggaggtgtc tttaagtgag ccgtatcgga aataaagtcg
     7381 ttgttatccc tgaaggagta acagttactc aagacggaaa caacgttaca gttaaaggac
     7441 caaaaggtga attgactcgt actttttcag cagatatcaa aatgaatatc gaaggaaacg
     7501 aagtaacttt cactcgtcca aacgacagta aagaaatgaa aacaattcat ggaacaactc
     7561 gtgcgaactt caacaacatg gttgtaggtg tcagcgaagg tttccaaaaa gcgttagaac
     7621 taatcggggt tgggtaccgt gcacaattac aaggatcaaa acttgtattg aacgttggtt
     7681 actcacatcc agtagaaatc acaccaccag ctggagtaac tgttgaagta ccagctaaca
     7741 cacaagtaat tgttaaaggt gcgaacaaag aagaagttgg cgaattagct gcaaacattc
     7801 gtggaactcg tcctccagaa ccttataaag gtaaaggtat tcgttatgtt ggcgaatatg
     7861 tacgccgtaa agaaggtaaa actggtaaat aatcgtttag aaaaattgca tagaagccag
     7921 cagcaggaaa aaggcatttg gctgaagttt accttcaaaa aaatacattt ttacttgtgc
     7981 cgcagcttct atttttcgaa acgtatttac tgcttgaaat agtagtacaa taaaaataaa
     8041 gaggtgacaa ttgtgattac aaaaccagat aagaacaaaa cacgtcaaaa gagacatcgt
     8101 cgtgtacgta acaatatctc tggtactgct gagcgcccac gcttgaacgt ttttcgttct
     8161 aacaaaaaca tctacgctca aattattgat gacgtagcgg gtgtaacgct agcaagtgcc
     8221 tctaccttgg ataaagaaat ttcaggtgga acaaaaacag aagcagcaca agctgtcggt
     8281 aaactagttg ctgaacgtgc agctgaaaaa ggcattaaag tagtagtctt tgaccgtggt
     8341 ggataccttt accatggccg tgtgcaagct ttagctgaag ctgctcgcga aaatggacta
     8401 gaattttaga agaaggagga ataccattca tggtttatat cgatccaaaa catttggaat
     8461 tagaagaccg cgttgttgca attaaccgtg taacaaaagt tgttaaaggt ggacgtcgtc
     8521 tacgttttgc tgctttagtt gttgtcggtg acaaaaacgg acacgtaggg tttggtacag
     8581 gtaaagctca agaagttcct gaagcaatcc gtaaagcaat tgaagatgcg aagaaaaact
     8641 tagttgaagt acctatggtt ggttcaacaa tcccacacga agtaatcggt gcttttagtg
     8701 gtggtagaat cctaatgaaa cctgctgtag aaggttctgg ggttgctgct ggtggaccag
     8761 ttcgtgccgt attagaatta gctggggtag cagatatcac ttctaaatct ttaggctcaa
     8821 atacaccaat caacgttgtt cgcgcaacag ttgaaggttt gaaacaattg aaacgtgcag
     8881 aagaagttgc agaacttcgc ggcaaatcag ttgaagaatt aatcggataa ggaggacaac
     8941 aataatggct gaattaaaag ttactttaaa acgcagtatt atcggacgtc ctcaaaacca
     9001 acgcgataca gttaaagcgt taggtcttac aaagattaac agttcagtgg ttaagccttc
     9061 taatgaagca attaaaggca tgatcaatac tgtttctcac ctagtggacg ttgaagaaat
     9121 ttaatttaga aaaatcataa ctagaacatt aaggaggtgc caaacctatg aaacttcatg
     9181 aattaaaacc tgctgaaggt tcacgccaag tacgtaaccg tgttggtcgt ggtacttcat
     9241 ctggtaacgg aaaaactgcc ggacgcggac aaaaaggaca aaaagctcgt tcaggcggcg
     9301 gtgtgcgttt aggatttgaa gggggacaaa caccattgtt ccgtcgttta ccaaaacgag
     9361 gattcacaaa cgtgaaccgt aaagactatg cagtcattaa tcttgatgta ttgaaccgct
     9421 ttgaagacgg agctgaagta actcctgttg ctttagttga agctggaatc gtgaaaaacg
     9481 aaaaagctgg aatcaaagta ttagctaatg gggaactaaa caagaaatta actgtgaaag
     9541 cagcaaaatt ctctaaagca gctcaagaag caattgaagc ggctggtgga tcaatcgagg
     9601 tgatctaatg tttagactat taaaagatgc ttttaaagta aaagacatta gatcgaaaat
     9661 cctatttact gtatttgtgt tgtttgtatt tcgcctaggt gcacatatta ctgtaccagg
     9721 cgtaaatgct gcttcattgc aaaacctttc ttctttgcca tttttcaaca tgttgaactt
     9781 ggtaagtggt agtgcgatgc aaaacttttc cattttctca atgggggttt caccatacat
     9841 tactgcttct atcgtcattc agcttcttca aatggacatc gtgcctcgat ttgttgaatg
     9901 gtcgaaacaa ggggaagttg gtcgtaaaaa actgaatcaa gcgactcgat atctgacatt
     9961 agtattggca tttatccaat ctgtcggtat cactgctgga ttcaatcaat acgctcaatt
    10021 aggtttcgtg aataatccta acgtagcaac ttacgtaacg atcggtttga ttctaacagc
    10081 cggtacgatg tttgttactt ggctaggaga acagatcaca gaaaaaggta tcggaaatgg
    10141 cgtatcaatg atcatttttg caggtatcat ttctagattg cctgaaagta tcagagaaat
    10201 ttatgaagat tactttataa atattgatcc ttcagacata tggaaatctg ctatttttgt
    10261 ggccatttta ctagtagcgg tcttgttgat cgtgacattc gtaacatttt tccaacaagc
    10321 tgaacgcaag atcccgatcc aatatacgaa acgcgtagct ggcgcgccta caagcagcta
    10381 tctgccgttg aaagtaaatg cagctggagt tattccagtt atctttgcta gttcgtttat
    10441 tgctacgcca aatgctgttt tacaagcatt gagcggtaga ttcggttctg aaaactggta
    10501 caaagtgatg atggagatct ttaactataa tacagcacct ggtgctacga tttatacgat
    10561 tctgatcgtt gccttcactt tcttctacgc ctttgttcaa gtaaaccctg agaaattagc
    10621 ggaaaacttg caaaagcaag gaagttatat accaagcgta cgccctggta aggggactga
    10681 agagtatgtc tcaagattac tgatgcgttt aagtacagtt ggtgcagtct tccttggttt
    10741 agtggcattg cttccaatca ttgctcaaat ggtttgggat cttccacaat caatcggatt
    10801 aggcggtaca agcttactga tcgttatcgg tgtagcttta gaaactgcga aacagttaga
    10861 aggcttaatg ctgaaacgta aatatgttgg attcattgag taagaaagcg tgttgaggtt
    10921 tcccctcaac acacgttctc acacatttat taggagggaa aacaatgaac ctcattttaa
    10981 tggggctgcc aggcgcaggt aaaggaacac aagctgaaaa aatcattgat gcttatggta
    11041 ttccgcacat ctcaacagga gatatgttcc gcgcagcgat gcaaaacgaa acagctcttg
    11101 gcctggaagc aaagtcttat atggataaag gtgcattagt tcctgatgaa gtaacaaatg
    11161 gaatcgtgaa agagcgacta gctgaaccag atacagagaa aggcttctta ttagatggct
    11221 ttccacgtac gctagatcaa gcaaaagctc tggacgcaat gctaaaagat ttgaataaaa
    11281 aaattgatgc tgtcatcgat atccatgttg gtgaagaaat cttggtcgaa cgtctagccg
    11341 gccgatttat ctgcagcaat tgtggagcaa cttaccataa aattttcaac cctacaaaag
    11401 ttgaagacac atgtgatcgt tgcggtgggc atgaattcta tcaaagagaa gatgataaac
    11461 ctgagacggt taaaaatcgt ctggctatca acatcaaaaa tagtgaacca atcttggctt
    11521 attataaaga acaaggtttg ctaaacacta ttgatggtgc tcgtgaaatc gatgctgtat
    11581 ttgcagatgt acaaaaaatc attgaaaaat agcacgattg cccgtagccg acttgcatta
    11641 tgcagttact cttgtcaagt tcaataaatt atgatagaat atgtcagtcc gacttctaga
    11701 gaatcctcta gatgatccat tttgagaatt ttgaggtggg aactgcagtt tccgccattt
    11761 tatcgtgtga aaatgcgaaa caagtacaag gaggtactgt gcgtggcaaa agaagatatg
    11821 attgaagtcg aaggtacagt cgtcgaaact ttgccgaatg caatgtttaa agttgaacta
    11881 gaaaacggac accaagttct tgctactgtt tcaggtaaaa ttcgtatgca ttacatccgt
    11941 atcttacctg gtgacaaagt aacggttgaa ttgtctccat acgatcttaa ccgtggccgt
    12001 attacttatc gctttaaata attgtactcc gtaaaactac aggaggtatt atcatgaaag
    12061 taagaccatc agtaaaacca atgtgcgaac attgtaaagt aattcgtcgt aaaggacgcg
    12121 ttatggtgat ttgcccagca aatccaaaac ataaacaacg tcaaggataa ttggaggtgc
    12181 tataaatatg gctcgtattg caggagtaga tattcctcgt gataaacgtg tagtagtttc
    12241 acttacttat atctatggta ttggtaacac tactgctaaa aagatcttag ctgatgctgg
    12301 tgtatctgaa gatgttcgcg ttcgtgaatt aacgaatgaa caaacagatg ctatccgtgc
    12361 agaagtagat aaattaaaag ttgaagggga tcttcgtcgt gaagtgaact tgaacatcaa
    12421 acgtttgatg gaaatcggtt cttaccgtgg aatccgtcat cgccgtggat tgcctgttcg
    12481 tggacaaaac acgaaaaaca atgcacgtac tcgtaaaggc ccagctcgcg ctatcgctgg
    12541 caagaaaaaa taattcttaa gtgaaggagg ttaaaacttc atggcagcaa aaaaagtgaa
    12601 tcgtaaacgc cgtgtgaaga agaatataga agcaggtatt gcacatatcc attctacatt
    12661 caataacact atcatcatga tcactgatac tcacggaaat gcattagcgt ggtcatcagc
    12721 tggttcatta ggtttcaaag gaagcaaaaa atcaactcct tttgccgctc aaatggcagc
    12781 agaaactgct acaaaagcag caatggaaca cggattaaag actgttgaag taacagttaa
    12841 aggacctggt tctggacgtg aagcagcaat tcgttcatta caagctacag gtttagaagt
    12901 gactgcaatt cgtgacgtga ctccagttcc tcataatggt gcccgccctc caaaacgccg
    12961 tcgtgtttaa tgagtgccgc tcattttgag ttttatcagt aacgtcattg aacaagacac
    13021 ttttcgtttt gaaaggggta aagataagaa tgattgagtt cgaaaaacca agaatcgcaa
    13081 aaattgatga agaaaaagat tatggcaagt tcatcgttga acctcttgaa agagggtatg
    13141 ggactacttt agggaattcc ctacgtcgta ttttattatc ttctttgcca ggtgctgcca
    13201 tcactaatat tcaaatcgat ggtgtactgc atgaattctc taccatcaaa ggtgttagag
    13261 aagacgtcac tcaaatcatc ttgaacatta aaggcttagc gctgaaaatg tatggccaag
    13321 aagaaaaaac ccttgaaatc gatattaccg gacctgctac agtaactgct ggcgatatta
    13381 tcgttgatag tgatgtagaa attcttaaca aagatatgta catctgtagt gtctctgaag
    13441 gagctacgtt ccatgctcgc ttaacagtga aacctggacg tggttatgtt caagcagatg
    13501 aaaataaaaa agaagatatg ccaattggcg ttcttccagt cgattcaatc tacacacctg
    13561 ttcgtcgtgt gaactatcaa gttgagaaca cacgtgttgg acaccgtgaa gattttgata
    13621 aattgacgat ggagatttgg acagatggtt caattgaacc gttggaagca atgagtttgt
    13681 ctgcgaaaat catgactgaa catttggata tttttgtgaa cttaactgat gaagcaaaaa
    13741 atgctgaaat catggtcgaa aaagaagaaa cacaaaaaga aaaaatgttg gaaatgacga
    13801 tcgaagagtt agacttgtct gttcgctctt acaattgttt gaaacgtgca ggaatcaata
    13861 ctgtacaaga acttacaaat aaatctgagc ctgaaatgat caaagtccgt aacttaggac
    13921 gtaaatcatt agaagaagtt aaagcaaaat tgcacgactt aggtttaggt ttacgtaaag
    13981 aagattaaaa ccttttacga aggagggaaa ccaacgtgag ttatcgtaaa ctaggacgca
    14041 catctagcca acgtaaagcg atgttgcgtg atttaacaac tgatttatta atcaacgaac
    14101 gtattgtgac tactgaagct cgtgcgaaag aagttcgctc aactgcagaa aaaatgatca
    14161 ctttaggtaa acgcggagat ttgcatgcac gtcgtcaagc tgcggctttc gtacgtaatg
    14221 aagttgccag cgtgcgtgaa gaaaatgagg acatcgtcat tgaatcagct ttacaaaaac
    14281 tgttcaacga tattgcacct cgttacgcag aacgtcaagg cggatacaca cgcattttga
    14341 agacagaacc aagacgcggt gatggtgcac caatggttgt cttagaactt gtctaaaatc
    14401 acactaataa catcacttta tagatgattc ttttagagtg ttatgatgtt ggaagatatt
    14461 tttccgagtc tagctcagcg ctgctcctca agcgaatatc gcttggggag taagcactca
    14521 tcataaagtg tttttttata ccttcaatta cctgtcatga cagatatgtt tgaaggcata
    14581 aaaaaacatt ttaagcgaga aataattgaa aattttgtct ttttttgata gtgttagtag
    14641 gaactttagg atgaagaagg agtaatcatg gagccaatta ttgaattaga aaagataaat
    14701 tataaatacc agccagatga tcttcgcccc gcattgaaag atgtctcctt tacaattgat
    14761 aaaggtgagt ggatcgctat tattggacac aatggttcag gaaaatcgac acttgccaaa
    14821 acgatcaatg gattgcttct gccagaatca gggatcgtga aagtgggaaa tcaaatattg
    14881 gatgaagaaa atatttggac gatccgacag atggttggaa tggtttttca aaatccagac
    14941 aatcagtttg ttggttcgac agtagaagat gacgttgctt ttggtcttga aaatcaaggg
    15001 attcccagag aagaaatgct agtgcgtgtg aaagatgcac ttgagaaagt tagaatggct
    15061 gagtttgctt cacgcgagcc tgctcgttta tcaggagggc aaaaacaacg tgttgccatt
    15121 gccggcgtgg ttgccttacg acctgatatc attattttag atgaagcaac cagtatgcta
    15181 gatccagaag gtcgtgaaga agtgatctct acgatcaaga agatcaaaga agaaagtcaa
    15241 ttgaccgtta tatctatcac tcatgatatt gatgaagcag ctaatgccaa tcggatacta
    15301 gtgatgagac aaggcgaact tgtccgtgaa ggtacaccaa aagaaatttt ttctgctggt
    15361 cctgaactga tcgacttagg tctagatcta ccatttcctg aaaaactgaa gagcgcactg
    15421 aaagaacgtg gggttgatgt gcccagtgaa tatatgacag aagaaaggat ggtggattgg
    15481 ttatggacat ccgttttgaa caagtagact tcacttatca gccgaataca ccatttgaac
    15541 aaagagcctt atttgacatc aatatgacga tcaaagaaaa cagttatact gcattagtag
    15601 ggcataccgg aagcgggaaa tcaaccttac ttcagcattt gaatgcgctt gttaagccaa
    15661 caagcggtac agtccatatt ggagaacgag atattcagcc ggatacggat aataagaatt
    15721 taaagcccat ccgaaaaaaa gttggcatcg tctttcagtt tccagaagca cagctattcg
    15781 aagaaacggt agcaaaagac attgctttcg gtcctaaaaa ctttggtgtc agcgaagaag
    15841 aagcactagt cctagcaaaa gaaacattag aacaagttgg gctggatgaa agctatttgg
    15901 aacgttcgcc atttgaactt tcaggagggc aaatgcgtag agtagcaatc gctggtgtgc
    15961 ttgccatgag gccggaagta cttgtattgg atgaaccaac ggcaggactt gatccccagg
    16021 gtagaaaaga aatgatggag atgttttggc gtcttcataa ggaacagcag atcactattg
    16081 tattagttac gcatttaatg gatgatgtag ccaattttgc agattatgtc tatgtgttgg
    16141 aaaagggacg aatcgttaat agcggggagc cgcaagaggt ctttcaaaac attgaatggc
    16201 taaaagaaaa acaactaggc gtacctactg ccaccgagtt tgcagaagaa ttaatggcta
    16261 aagggatgaa ctttgctacc ttgcctttga cagctgaaga attagcagac gctatcgtcc
    16321 attatgctgg aggaaatgcc catgatgaat aaattgatat ttggtcgtta tatgcctgga
    16381 gattccttga tccatcgatt ggatccccga gctaaattat tagccagttt ttattttatc
    16441 ggaatcattt ttttagcaaa taattgggaa acgtaccttt tgttggcatt tttcacatta
    16501 ctttctgtct tcttgtcaaa agtagatttg aaatttttta tccgaggcat tcgcccattg
    16561 atctggctaa tcctcttcac tgtagctttg caggtcttgt ttacaagtgg cggagaaact
    16621 tattggagtt ggggtgtttt ccatatcact gaatttggtg tgatgaacgg cctctttatt
    16681 ttctgtcgat tcgtgttgat catttttatg tcgactctac tcacgcttac tacaccgcct
    16741 ttggaattat cggatgcgat tgagtacttg cttcgtccat tgaaagccgt acggttccca
    16801 gtccacgaaa tttcattgat gctgtcgatt gctcttcgtt ttgtgcctac attgatggac
    16861 gaaacagaaa agatcatgaa cgctcagcgc gctcgcggtg ttgattttgg tgaaggaaac
    16921 ctagttcaga aaatgaaagc agtcattcct ttgctgattc ctttatttgt cagcagcttc
    16981 aaccgtgcgg aagatcttgc gactgctatg gaagcaagag gctacaaagg tggggaagga
    17041 agaacgaaat atcgtgtatt gcattggcat aatagagaca cgatggttat cattgctttt
    17101 gtattattaa ctgtgctact gatctttttg agaggataaa cgttcagtgg gccgctatct
    17161 attcttttct tgctgatctg tttgcaagaa aggatgcgag cagtgtatga caagaaatat
    17221 tagggtctgt ggcagactaa ggtcacaggc ctctttcttt tatctgaaaa gaaagaacga
    17281 aaaaatttta taaaaaaatc attattccag ataacctttt aggaacaaag gagagaaaaa
    17341 catggtacgt tacaaagcaa tcattgcata cgacggaaca aattttaatg gattccaaaa
    17401 acagccaaat gggcgtactg ttcaagaaga agtagaaaaa acattacaaa aaatggcaaa
    17461 tggaaaagaa atcacggtct ttggttcggg aaggacagat gccggagtac atgcgattgg
    17521 gcaagtaatc cattttgatt atccagagga gcgtccgtta gaacgaatgc gtttcgcgct
    17581 agatacccag tcaccagagg atattgctgt aagacaagta gaaattgttt cagaagactt
    17641 ccacgcaaga tatttggtca aagaaaagac gtatcaattt cgagtggata tagggaaacc
    17701 acgcagtcct tttcgacgcc attatgcaag ttattttcct taccctcttg atttggaaaa
    17761 aattcagcgg gcgcttcctg atttattagg gactcacgat ttcacctctt tttgtgcatc
    17821 aggaagctcg atagaggata aagtacgtac gatctacgag gctaagatgg aggtaaatga
    17881 aacaaaagac gaattgttgt ttacatttag gggaaatggc ttcttgtata agatgatacg
    17941 gatcttagta ggaacgcttt tgaaaattgg caatggccga ttgcctgaag atagtatccc
    18001 agatattatt gcgaaaaaag atcgtaatgc agcaggacct acagcacatc ctgaaggact
    18061 ttatttgtat gaagtgaaat atgaataaga ggttgtgaca gaagtggtca gcttcaagaa
    18121 ataagaagga atttacgaaa attacttctc aaaattttgc aaaatttcgg cttattttcg
    18181 aaggagctac ttctggaaca ccgtgaatca gaggttgtga aaaagctgcc ctgcatcaag
    18241 taataagaac aaccaaacaa aaatagctcg tcatattttt cgtttggttg ggcttattac
    18301 cgcagatgca agcttttgaa caccgtttaa ggaagaggtt gtgaaatcag cgttttgacc
    18361 tgagtattag agaaacaaac ggaaaaatag ttcctcatat tttgccgttt gttgagctaa
    18421 taccgaaggt cagctgattg aacaccgttt aagaaagagg tcgtgagcct gtcatttaac
    18481 tctgagtcac aagtaacaat ttcaaaaaac agcttctcat ctttgatgaa tatcgtgact
    18541 tgtggacgag gcgttgataa gtgaatcatc tttttgttac ctattttttc tgtaaccaaa
    18601 aaacgatgga acgttgtttt gattgccctt aatgaggaca gtcggaacag gtttgtcatt
    18661 tttatattta aatggcacaa atttttctaa ttatttgtca tttagcgctg aaaatttttg
    18721 ctatttatct aaaaaagtga taactactta agatgaagca gaatatttac gccatacggc
    18781 atacaaccgc gaaaaatata aaaaaaagaa aacgattgaa cgcgtttttg cagtcgcaaa
    18841 ggaaaagcat ggaatgcgct acatcaagta tcgagacttg gaaaacaatc aggtgtatac
    18901 gctgattgtt tttgctgcaa tgaatctgaa aaagttgtcc acttggcagt tggaacatat
    18961 gtcttcatat cttttattat acaaaaatag caaaagcacc agtctacact cttagtaaag
    19021 aggaaactga tccttttgtc tacattctta aagtacttaa actaagtact ttattttact
    19081 ctttaggatt gatacagtag catctatccc tttattaata atcttttatt tttttaaata
    19141 aagatgttgt ctccatcttc aggcactaaa actttttcgg aaattccttt ttctttattg
    19201 aaagacttta attcttctct tgacagtccc caatggttta cagcttccat atgaacggca
    19261 ataattgtgg aatctggtat tgctttatgc aattcatata aatcgtcttt ccccataata
    19321 agagattctc caactaaaaa atgattatct ccagcatttg ctactatgat ttctggatta
    19381 tactgatcaa tagtttgttt aacagcttca taccaaactg tatctcctgt aaagtatagt
    19441 gtcttctcag tcgaatgttt gaaaacaata ccgcaaacat tacctacata attcaagatt
    19501 tctcctctgc catgtttgcc ttttgtttta accaattgta tagattcaaa aactgtgtct
    19561 tctcgtagta cctccacatt tttaaaacca tatagtcgaa tttcattcgc atcattttca
    19621 tcttgcacaa ataattttat ttctttaggt aggacctctt tcgctatatc gtcccaatga
    19681 tctagatgta aatgtgttac aataacagca tcaataccgt tgataatatc ttcgactggc
    19741 attggcaaat caactagagg attcatttga tcttttcttg gtgatggaaa aggacctttc
    19801 gaaataaatg gtggaagtgt tcctttttcc gctaacattg gatcaattaa aaatttttta
    19861 cctccatagt ctaagatagc tgttgcatta cgaatgtgat gtatattcat aaacgttgtc
    19921 tcctttttgt tgatatgaaa tatttttagt ataattcaat tccatacaat aaataaaccg
    19981 aacaaaaaaa gtcttttact atttttactt aatttatgaa aggttgttta ccatgttaga
    20041 ccaaacagat ataaatatca ttgaggaact atcaaaaaat agtcgaatca caatgaaaga
    20101 acttggagaa aaagtacatt tgactggacc cgctgtctcg gcgagagtga caaaactaga
    20161 ggaaagtgga gttattgaaa attatacagt aaaattaaat atggaaaagc tagggttttc
    20221 gattcacgct tttttaatta taattacgca aaatttaaat cataaaccct atttatcatt
    20281 tattaaaaat catgaaaaat tcttgattag aaattataag attagtggag ggggctgtta
    20341 tcttttggaa tgtagattta cttcaaaaaa atcaatgaac agctttttgg aacagcttaa
    20401 tgaatatgcg aattataaat tatctattgt tatagattaa taataaaact agcagatggt
    20461 aaaaatacta atatgcgaga cacacattca accataacta tataacttat caatttaaaa
    20521 ctaaactgat ggaaacaagt aaggcaaaaa cattt
//
LOCUS       DACCYH010000049        20384 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.050, whole genome shotgun sequence.
ACCESSION   DACCYH010000049 DACCYH010000000
VERSION     DACCYH010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.050"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            63..3452
                     /locus_tag="IVY85_001722"
     CDS             63..3452
                     /locus_tag="IVY85_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP8044740.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            3455..4876
                     /locus_tag="IVY85_001723"
     CDS             3455..4876
                     /locus_tag="IVY85_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8044741.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            4873..6750
                     /locus_tag="IVY85_001724"
     CDS             4873..6750
                     /locus_tag="IVY85_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8044742.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            6842..7684
                     /locus_tag="IVY85_001725"
     CDS             6842..7684
                     /locus_tag="IVY85_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8044743.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(7836..8711)
                     /locus_tag="IVY85_001726"
     CDS             complement(7836..8711)
                     /locus_tag="IVY85_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8044744.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            8918..9949
                     /locus_tag="IVY85_001727"
     CDS             8918..9949
                     /locus_tag="IVY85_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP8044745.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            10038..10343
                     /locus_tag="IVY85_001728"
     CDS             10038..10343
                     /locus_tag="IVY85_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP8044746.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            10522..12537
                     /locus_tag="IVY85_001729"
     CDS             10522..12537
                     /locus_tag="IVY85_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8044747.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            12539..12823
                     /locus_tag="IVY85_001730"
     CDS             12539..12823
                     /locus_tag="IVY85_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8044748.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            12849..14222
                     /locus_tag="IVY85_001731"
     CDS             12849..14222
                     /locus_tag="IVY85_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8044749.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(14305..16320)
                     /locus_tag="IVY85_001732"
     CDS             complement(14305..16320)
                     /locus_tag="IVY85_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP8044750.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16342..17556)
                     /locus_tag="IVY85_001733"
     CDS             complement(16342..17556)
                     /locus_tag="IVY85_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8044751.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            17777..18721
                     /locus_tag="IVY85_001734"
     CDS             17777..18721
                     /locus_tag="IVY85_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8044752.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            19017..19961
                     /locus_tag="IVY85_001735"
     CDS             19017..19961
                     /locus_tag="IVY85_001735"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8044753.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         6966 a         3730 c         3881 g         5807 t
ORIGIN      
        1 gtttatttta ataattttca ctattcacgt caaaacagaa aaaaagaaaa aggaggggaa
       61 aaatgacgac cacagggaag aaactgaaag ttattttcat gctgataata ttgagtttat
      121 caaactttgt gccattatct gcaatagcag acactacaga tgatccaaca gttttagaaa
      181 caatttcagc tgaagtcatt tcggatcagt ctggaaaaaa agcactgaac atcaagctaa
      241 atgcgaataa caccagtgct gaaaagatag aaaaagaaat tggtctagtc gaaaattact
      301 taagtgatgt ggaaagaaaa gaaggagatg gctatgctta tcaggtaaat agcgggaaaa
      361 ttacgttgga aatctcatca aacactaaac aaactatcga tctgagtttt ccaatcgatc
      421 cagcacttta ccacagccag gcaaacaagc tgatcgtcga taataaagaa tatgacatta
      481 ttgatgagac agaaaataag aaagatacag atgtgtcagt accaaagcca gacgaaatag
      541 aagaagaatc atcaaaagaa aacgaaaatt ctgtcagccc atttacattg cctacattat
      601 ccttgccagc tgtgagtgtg ccatctaatc aaacgattcc tacagaatat acaacagatg
      661 atcagggcac ttatcctaaa gccagttggc aacctacagg aaatacaaat gttcttgatc
      721 atcaaggcaa taaaaacgga acaaatcaat gggatggtat aaattcttgg aatggagatc
      781 ctaatgatcg gacccattcg tatatcgaat atggaggaac cggtaatcaa gcagactatg
      841 cgatacgaaa gtatgcaaag gaaacaagta cacccggatt gtttgatgtt tatttgaatg
      901 ctcgtggaaa tgtacaaaaa gatatcacgc ctcttgatct cgtattggtc gtagactggt
      961 caggaagtat gaacgacaat aatcggatcg gtgaagtaaa gattggtgtc gatcgttttg
     1021 tcgatacttt agcagatagc ggtatcacag acaaaatcaa tatgggatat gtcggctact
     1081 caagcgaagg atatagctac agtaacggtg cagtacagat gggttcattt gattcagtga
     1141 aaaatcaagt aaaatccatt acaccttcac ggacaaatgg tggtactttt acacaaaaag
     1201 cactaagaga tgcaggaagc atgctatccg ttccaaatgg acataaaaaa gtgatcgttt
     1261 tgctgacgga tggtgtacca acattttcct ataaagtaca gcgggtacac gcacaatcaa
     1321 gcagcaatta ttacggaact cagttttcta atacgcaaga tcggccggga aatacttctc
     1381 taatctcaag aatctatgat gcacctgacc aaaacaatct atccagaaga atcgacagta
     1441 cgtttatcgc aaccatcgga gaagcgatgg cactcaaaga acgaggaatc gaaatacatg
     1501 gtcttggcat ccaacttcaa agcgatccgg cagctggtct ctcaaaagca gaagtagagt
     1561 ctcgtatgcg acaaatggtt tcatcagatg aaaaaggcga tctttactat gaatcagctg
     1621 atcatgcaac agatatctct gaatacctag ccaaaaaagc tgtacagatc tcagcaactg
     1681 taagcaatgg acaaataaat gatccaatcg cagaaccatt catttatcag cctggtacac
     1741 tttcagtcaa gagtgtgggg acaagtccta caacggtcac tccatctatt tccatagaag
     1801 gaaataccat caagagcaat cagatctatt taggaaaaga ccaagaaatc caaatccatt
     1861 accaagtgag aatccaaaca gaaaatgagg acttccatcc aaatttctgg tatcaaatga
     1921 acggcaggac aactttccag ccaaacattg ataccaatga attagctgaa ttcggtatac
     1981 catctgctaa agctcccgga gtcagtcttc acatcaaaaa gttatgggaa gaatttgaca
     2041 acaatctagc tgatcgtcca gatcaagtta cttttgagat tcaacgggaa catacgacaa
     2101 atgctgcagc ttggaaaaac ggatatattc gaatcattaa accagctaaa gatacaacaa
     2161 atacgtggga acgtgcagac attgacaaat tatctgcaaa tagcggagaa agttatcaag
     2221 agatattatc actacctcaa tacaataatc aaggtcaagc attcagttac caaacaatca
     2281 aagaattacc tgtaccagga tacgattctc aacaaataga tgcaatgaca tggaaaaata
     2341 ctaaacaatt cacaccgtta aacttgaaaa taacgaaaaa ttcctctaca ggtgaaaagg
     2401 atcttattgg cgctgttttc aaattaacag gagattctat tgatacttta ctaacagatc
     2461 atggcgacgg aacctattct cttccagaaa atgtcaaatt gcaaaaagaa atgacctata
     2521 cgctgacaga aacaaaagct ccagaagggc atggattaag caaaaagact acttgggaaa
     2581 tcaagatcgc ttctgatggt acggtaacca ttgatggaaa aacagtcact acttccgatg
     2641 atacgatcca gttgactatt gaaaatcctt ttgttgaagt tcctgtagca gtacgtaagt
     2701 atgcgatgca agggacggac aaagagataa atcttaaagg agcagcattt tccctacaga
     2761 aaaaagaagc aaatggtact tatcagccaa ttgacagcca aacaacgaat gaaaaaggtc
     2821 ttgccagttt tgattcactc acacctggta aatatcgagt cgttgaaaca gctggtcctg
     2881 ccggatatga tacttcgccg ggaaattatg aattccaaat cgataaatat ggaaaaatca
     2941 tttacacggg aaaaaatacc gagatgacaa ataatgtatg gacgctcact catcaaaatc
     3001 gactaaaagc gtttgatcta acggtacaca aaaaagaaga caacggacag acattaaaag
     3061 gagcaaaatt cagactgcag ggaccagaaa tggacttaga atcgccaaaa gatggacaag
     3121 aaacagatac ctttctattc gaaaatttaa aacctggaac ttatacgctg accgaaactt
     3181 ttacaccaga aggataccaa ggtctaaaag agccagttac tatagttata cacgaagatg
     3241 ggtcaattca agtggatgga caagatcatg aatctgttct gtcaccagga gccaaaaaca
     3301 accagatttc tttagacatc acgaatcagg caaaagtacc attacctgaa acgggaggaa
     3361 ttggccgttt aggaatctat ctagtaggga tgattggttg tgcgttttct atttggtatc
     3421 tttttttgaa aaaagaaaga gggggcagct aaatatgaaa aaacttggtt ggcttagtat
     3481 gtgtctcttc ttgttactat ttaaaccagc ttttactcag gtagcaacag aaacagaaac
     3541 agaaatggtt cagattactt tacacaaatt gcttttccca aacgggcaac tgccgaaaaa
     3601 tcatccaaat gacggacaag aaaaagcttt attacaaacg tatcgaggat taaatggtgt
     3661 cacattccaa gtttatgatg tcacagattc tttttaccat ctacgggaaa agggcaaaac
     3721 ggtagaagaa gcacaagcag agatcgcaaa aaacggtgcg tcttccggta tgtttaccgc
     3781 agaagcaaca actacaactc ttaacaacga agatggtatc gcttcttttt ctctggccgc
     3841 taaagatcaa gaaaaaagag ataaagcgta tcttttcatt gaatccaaag taccagaagt
     3901 cgtcaaagaa aaggcagaga atatggtagt tgttcttcct gtacatggac aaaacaatca
     3961 aaaactttca actatccatt tgtatcctaa aaatgaagaa aacgactacc ctgatccacc
     4021 ttttgagaag gtattagaag agcctagaaa tgattttacg attggtgaaa aaatcactta
     4081 ttccttgcat acgacaattc ctgtaaatat ccttgactat caaaagttcg aattgtcaga
     4141 tagtgcggat gaagcattaa cgtttttacc taatagttta acgatttcat cgaatggaga
     4201 aaagctgaca gaaggctttg tcatacacaa gaaacctcac ggatttgatg ttttattttc
     4261 gatcccttcg ttggaaaaat atgctggaaa aaaactgacc atttcttatc agatgcagct
     4321 aagcagtaca gcacaggcga acaaggaaat caacaacaac ggaacactgg attttggttt
     4381 tggtgtcagt acaaagaaag tctctgtata tacagggagt aagcaatttg tcaaaatcga
     4441 gacaaataaa ccagataaac gattagctgg cgcagtattc cttattaaaa acaaagcagg
     4501 aaattacctc cagcaaacag ccaacggata caagtggaca aagaacgaat cagatgcgct
     4561 tcacctgatt tccgataaaa atggcgcttt ttcaatttcc gggttgaaaa caggaagtta
     4621 tcgattaaaa gagatcgaag caccttctgg ttatatttta agtgaaacag aaattccgtt
     4681 taccatttca acttttcttt ctgaggataa agaggcggac agtatattga aagtagtcaa
     4741 taaaaaagaa aatagccgtc catttcttcc aaaaacaaac gaaacgaaaa atacactttt
     4801 aggcgttgtt ggtatggtat tcgcaagctt tgcaatctgg ttgtttatca aaaaaagaac
     4861 aggagtgaaa aaatgaaaaa tcataaaaaa ataaacgtta tgttaggagt ccttttcctt
     4921 attttaccat tactcacaaa cagcttcggc gcaaaaaaag tgtttgcaga ggagacagca
     4981 gctcaagtca tccttcataa aaagaaaatg actgatttac ccgatccttt aatccaaaac
     5041 agcgggaaag aaatgagcga attcgatcaa taccaaggat tagccgatat ttcattttca
     5101 gtttataacg tcactcaaga attttatgcg caacgagata aaggagcgtc cgtggatgca
     5161 gcaaaacaag cagtccagtc tttgactcct ggtacaccag ttgcttcagg aacgacagat
     5221 gctgatggaa atgtcacttt atctttacct aaaaaacaaa atgggaaaga tgcagtctac
     5281 acgatcaaag aagaaccaaa agacggagtg tcagctgccg caaacatggt tttagctttc
     5341 cctgtatatg agatgatcaa acaagcagat ggctcttata aatacgggac agaagaacta
     5401 gatactatcc atctctaccc taaaaataca gtcggtaatg atggaacgtt gaaagttaca
     5461 aaaatcggta ctgccgaaaa cgaagcacta aatggagcag aatttattat ttctaaagaa
     5521 gaaggaacac caagcgtcaa aaaatacatc caaagtgtca cagatggatt gtacacttgg
     5581 acaactgatc aaaccaaagc caaacatttc attactggtc attcttatga catcggcaac
     5641 aatgactttg ccgaggcatc tattgaaaaa ggccagttga tcgttaatca tttagaagtt
     5701 ggaaaatata atttagaaga agtaaaagct cctgataatg cggaaatgat tgaaaagcaa
     5761 acaatcacgc cttttgagat cctggcaaat agccaaacac cagtagaaaa gaccatcaaa
     5821 aatgatacgt ctaaagttga taaaacaaca cctcaattga atggaaaaga tgtcgcaatc
     5881 ggtgaaaaaa ttcaatatga gatttctgtc aatatcccat taggtatcgc tgataaagaa
     5941 ggaacgcaaa acaagtacac aacattcaaa cttatcgata ctcatgacgc tgctttaaca
     6001 tttgataatg attcttcagg aacgtatgct tatgccttat atgatggaaa taaagaaatc
     6061 gacccagtaa attattctgt cactgagcaa acagacggat tcacggtttc agttgatccg
     6121 aattatattc cttcattaac tcctggcggt acattgaaat tcgtttacta tatgcatttg
     6181 aacgaaaaag cagatccaac caaaggattt tctaaccaag caaatgtcga taacgggcat
     6241 acaaatgatc aaacaccacc gtcagtcgat gtcgttactg ggggcaaacg atttgttaaa
     6301 gtagatggtg acgttacatc agaccaaaca cttgctggag cagaattcgt cgttcgtgat
     6361 caagatagtg acacagcgaa atatttatcg atcgacccat ccacaaaagc cgtcagctgg
     6421 gtatcggcga aagaatcagc aacggttttt acaaccacaa gtaacggttt aatcgatgtg
     6481 acaggtctaa aatatggcac gtactatctg gaagaaacga aagcgccaga aaaatatgtt
     6541 ccattaacaa accgtgtagc atttactatc gatgaacaat cttatgtaac agcaggacag
     6601 ttgatttctc ctgaaaaaat accaaataaa cacaaaggta cacttccttc aacaggcggt
     6661 aagggaatct atgtgtatat cggtgcagga gtagtccttc tactgattgc tggactgtac
     6721 tttgctagac gcaagcacag tcagatttag tctttttgca agaattggaa aaaggagcag
     6781 cggattacgc aaatgctcct ttttctttta ttatcaaaca gtcatcacaa ggagaacaca
     6841 aatgaaacca aagaaaagga aacttctaga tctgctgatg cttttcttgc ttctctcagg
     6901 aatagggata ctagcttatc catttgttag cgacgcgctg aataattatc tggatcagca
     6961 gatcatctct cattatcagc aacaagcggt caaagaaaat gaagaagtca tggctaaaat
     7021 ccaagaaaac atgacaaaga aaaaccagca attagcaaaa gaaggtggaa atccaggtgc
     7081 agatcccttt actaagaaaa aagaacctgc gaaaaaggat agaacctatt ttgagaaaca
     7141 tacgatcggt attttgacga tccctgagat caatgtccat ttgccaattt ttgatgaaac
     7201 gaatactgtg ctgctagaaa aaggtgcctc attattggaa ggaacgtctt atcctacggg
     7261 gggagaaaat acacatgccg ttatttccag ccatcgagga ctgcctcaag caaagttgtt
     7321 cacagactta ccgaaactga ctttaggtga ttctttctat atcgaaatca atggacggac
     7381 actcgcttat caagtcgatc agatcaagac ggtagaaccg actgatacaa aagatctgag
     7441 aatcgaaaaa gggcaggatt tcgtgacact cctaacttgt acaccttata tggtcaacag
     7501 tcatcgctta cttgtcagag ggcaccgggt tccttatcat gcaaaagaag ttcaaaaaga
     7561 agtacagaaa gtatccgaga acaaacgatt tttcctgtat ggattgttag ctgcatgtat
     7621 actgattttc ctccttctta tatatctttt tcgtcaatcg ttcaaaataa gaaacagaag
     7681 ttagctattc ctcatttcta taatatatct attcgaatcg ttccgtaaac aaacaggctg
     7741 aggtttgaaa aagaaaactt cagcctgttt ttatgatgat atcaaagaga gcctatcaaa
     7801 cactatcttt cttattacta aatgaggatc ttttcttaag ttttccgata ttctttaaga
     7861 aggccttttt gcatcaattc ttgtttgttt ttcaagttgc ggatattctc ttcaaaattt
     7921 tgagaatata tcactgagaa taacgtattt aatacgtaat cgaaggcaat ttgagaggag
     7981 aaagtcgcta ctttagcaaa atcatattcc tcctgcgata taacaagaca catatcagcc
     8041 tgtttggcca tttctgaatg ctgatttccg gtaatcagca aggtaggggc ctctacatgc
     8101 ttcaaatagg tcattattcg ttcataatga tggatgcgac cgctgtagct aataaaaatg
     8161 gcgcaatcct cgctagtcaa attggctgca ttccaagaag aatcactgta ttcttcagcc
     8221 atgatcaaaa acttattcaa cttgaccatt ttattctgga atttccttgc cgtgatttga
     8281 gaatcacctt ttgcaaaaag aaagattcgc tgtgccttgt tcaatacttt accgatttga
     8341 tctaaatctt catgttcaat ctgcaattgt gcttttttta ttgactggat cgataaatcc
     8401 gccattttct ttgcaatttg ctgagtgcta tcttcacttg aaaaaggaaa attaggatca
     8461 atcgtttgat tgaactgagc aatcaattga tgaacttccc gccccagctc gaaacggaat
     8521 tctttaaatc cgctgaatcc catttttttg cataaacgaa tgatagctga atgggaagta
     8581 tacgtagctt ttgctaattc ttcaatcgtc atgtagactg cagcttcgat attcctacgg
     8641 atgtaatcga caatccgttt ctctgtcgtg gtaaacaact cttgttttgt tagtttttct
     8701 tcaataagca tgatctgacc tccaaattct atcttactga taaacacttg gttccgctac
     8761 tatctttttg atttttatcc ttactttgaa cattaatttc accaaaccga atgataacaa
     8821 gtgtttttct gaactttttt accagaatag ctattataaa tactttctgc gcttttctag
     8881 gatatactga aaacatcaat aaaaggaggc accactcatg cttacaatcg cttatcttgg
     8941 caatggaaag agtacaaatc gctatcattt gcctttttca agaaaactta cgaacaaaat
     9001 caatatcaaa aaaatttttt ccccatctga accagtgtgg aaaacattcg aagacatcga
     9061 atatactgat cagctgtctg atctttggga tgatcctgag atccaattag ttgtcatcac
     9121 tacacctagt caatttcatt atgaatatgg aaaaatggca ctcgaacatg gtaagaatgt
     9181 actagtagag aaaccttttg cagaaacgtc aaaggaagca gaagagttat ttgcattagc
     9241 aaaagaaaaa ggtctattta tccaatgtta ccaaaacaga cggttcgatt ctgacttctt
     9301 gacagtccaa aaagtaatcg aaagcggaaa attaggggat attttagaag tagaaatgca
     9361 ttatgattat ttcagaccgg aagtaccaga aaatacgcat gaattttcta ctgcgaacag
     9421 ctatttatat ggacatgctt gtcatacagt agatcaagtt ctttcttatt tcggcgaacc
     9481 agatgatatc agctatgatg tacgtcaact tttagggaaa ggacgaatga atgattactt
     9541 cgatctggat tttttctatg gaaatatgaa aatttcgata aaatccagtt atttccgcat
     9601 caaagaacgt ccaagtttcg tcgtttacgg aaaaaaaggg atgttcgtga aacaaacaaa
     9661 agatcgacaa gaagaacatc tgaaaatgtt ttacatgcct gatcatgaag actttggtat
     9721 cgatttgccc gaacattatg gtgtattgac ctatatagat gaacaaggga gttatcatga
     9781 agaaaaagta atttccgagg ttggtgatta cagccgtgtc tacgaaggga tttacgagac
     9841 attagttaat ggcgcagaaa aagtcatcaa agatgaagaa acaatcttac aaatgaaaat
     9901 tttggaagaa ggaatcaaaa gcattcaaga atcagaagga aaaatatgac atatttgtaa
     9961 gaatcgggtt tgttttttga cgcataaact gtcttattgt acgatgaaaa agtatagaac
    10021 tactaggagg aaccaacatg acaaattatg acggtgaaaa acacaaacga ttaagaggtt
    10081 caatggcccc taaaacagaa gaagaagtgg ctttagcagg aactaattct ggtgaaacag
    10141 aagacaaaga agaatcatta gctggcacaa actctggtga aacagaaaat aaagaaacag
    10201 ctttatctgg ttcaaattct gtagaagagg aaacaaagcc taatgagccc aatcactttg
    10261 aaaaagatat cgaaaaagcg gttgaagatg tcaaagaaaa atttgaagaa ctgacctcaa
    10321 aaaataaaca tgatcatcat tgatcggacg aaatcaattc ataaaagcag gagacatttg
    10381 gtcacctgct ttttttctgt atccattcga tagaaatcgt tcggtacagt cgtaaaaaaa
    10441 atccaactgt aagttgtacc aaacacccct cgatttaaaa gcgctaccat actagaatat
    10501 aagtaggagg tgaacagcgc tgtgaactac aaaattagtt atctcctgaa aacaccaaga
    10561 gaaatcagtc tagaaaaagc agcagagatc atcgaagtaa atacaagcga ggcgatcaaa
    10621 caattaactc cttttgccga atatgtggat gatacccgta tccgtataaa gaaattccga
    10681 gcaatcgact gggcagaaaa atttttggat attactcgat ttgaagctgt gtataccgaa
    10741 caagagcgaa gagatcttct ttatttgatg gtttattcga ctgcaacgtc tgtttctgtt
    10801 tttcattttc aagattttct tcaggtttct aaagggacag tcctgtcaga tatcaaaaag
    10861 ataagaaatg aattagaagt ggaagattgt cagctgtcgt atactaggaa aactggtttt
    10921 aggctgctag ggtcagaagc aatattaaga agagaagcaa aaaatcgcac tgctcgtttg
    10981 atgcagtcac ttgctggaag atttgggctc acgtatttaa ccgcaaccct ttccattgat
    11041 tattacgcca aagttagaga tttgctagat aaaacgattt cactatcaga tcttcgattc
    11101 gcaccaaatc gaatggatga attagcttat tatttgagtt tgacaaccat tcgaattgga
    11161 aaaacatcca tggaactggt aaaagatgct gaactgttgg cttctcttgc agtcactgac
    11221 attagtcgac aagtattgac tggccttggc tattccgaac atcaaaattc ggagattata
    11281 tttttctctc tttgcttagc atcgatcgtt gaaggagatg tccaggagcc tgcacttgat
    11341 tttttgctgg aatgttcttc agaagtcatc catcgcatgg aattcctgat ggccgtagaa
    11401 tttgcttcgt ttcgtgaatt attgatgaat gtcttttatc acttggtccc tgcctatttt
    11461 cgtatttcct attcttttta tttacctaat gtgatgatcg atcaaattaa gcatcaatat
    11521 gcttccatct acgaaatgac aagaaaagca ttacttccgt tagaaagacg aatagggaag
    11581 agcatccctg aagaagagat tggttttttc actatactat ttggcggaga gatcagaaaa
    11641 gcggacgcag aagaaagaaa tagaaaaata agagccgtga tcgtctgtcc aagtggcatt
    11701 agctcttcat tgatcttaaa aagtgaactt cagcaattat tcccgatgat cttttttaca
    11761 gagactaatt cagctgaccg cttacacgag gttgacgaaa cttcttacga tattattttt
    11821 tctactgtac ctctcacact tactgataag aaaaagtctc tctatgttct gcatccaatc
    11881 atgactgact tggaaaaaaa tcagctgatc aatcaagtac aaagagattg gctgatacca
    11941 ggattttcaa tgccttctgc ccaagaattg ttgtcggcgc tactgcctta tatcgaatta
    12001 aaagaaggcg tagacgaaga aaagctctat caagtattaa atcgtaaaat gaacaaactt
    12061 ttagaaaaga aggaggatca acgtcctatg ctatctgaat tattaacaga agaaatgatc
    12121 caattatcgg atcaaccaaa aaactggcaa gaagcaatca catttgccgc acagccgcta
    12181 ttatcagaaa ataaaataga agaaagttat atcaaagcta tgatcgagcg cgtggagcag
    12241 tatggtgcgt ttatccatat tggcgatcac atcgcattac ctcatgctcg tccagaagat
    12301 ggcgtaaacg aagtagggat gtctttgctg aaacttcaac aacctgtgga tctgcttgat
    12361 aatccagccc atcccattag tttgttcatc tgtttagctg ctatcgataa tgaggcacat
    12421 cttcgggctt tggcaaattt aacaaagcta ctttctaata aacaaaatct tcaagattta
    12481 ttacacgcat caacaaaaga agaaatatta gcgatcataa gaaaaggaga cgaatgatat
    12541 gaaaattttt gcagtatgtc aaagtggatt aggaacaagt tttatggtag agatgaacat
    12601 caaacaagca cttgcagatc ttggagcaga tgtggacgag tttgaagtaa gtcatacgga
    12661 tgttggaagt gtatctagcg atatggctga ttatttcttc attgagcatt ctttgaaaga
    12721 tacgctaact tctatacctg atgaaaagct cgtacctttg caatcgatca ttgacagcga
    12781 agaagtaaaa gaaaaagtaa cgaagatctt gtcaaaagaa taaaaatgat tcaagggagg
    12841 aactatcaat ggataaattg ttgaatttgt ttgtgtcaat cgtcagtcag ccctccatcc
    12901 tcgtttcttt gattgcactg cttggtctcg ttcttcagaa gaagaaattt tcagaagtga
    12961 tccaaggaac tatcaaaaca tttgtcggat tcctagtact gatcggcggt gctggtttaa
    13021 tcagtaattc actaacccct tttgcttcga tgttccaaac agctctgcat actcaaggag
    13081 tagttcctag caatgaagca gtagtggcga ttgctttgca acaatacggt acacctactg
    13141 ctttgatcat gctagtaggg atgatcgtga atattttact tgctagattc acaagatata
    13201 aattcatttt tcttacaggt caagcaatgc tttatgtttc ttgtctaaca gcagtcattt
    13261 tagtaagcgc ggggtttact gcaaatttgt ggatgatctt attaggcggg gtcttcgaag
    13321 gaacactatt aactattacg cctgctcttt gtcaaccgtt tatgaagaac atcactggaa
    13381 acaatcaagt tgcgttaggg cataccggaa atatcggtta tgcttctagt ggatggatcg
    13441 gaaagttttt tggaaacaaa gaccgttcta cagaggacat aaagattcct aaaagtttca
    13501 gctttttgcg agattctact gtatcgatca tgctcttgat gtcgatcgta tatgtcgcac
    13561 ttgctttgtt agctggtcct ggatttgttg aaagagaact aagcaatgga gacaacgctg
    13621 taatctttgc actgatccaa gctggaacat tcacagctgg gtttgttatc gtccttcaag
    13681 gggttcgaat ggtgttggca gaaatcgttc cagcattcca agggattgct aaaaaactcg
    13741 tacccgattc taaaccagca ttagatgttc ctatcgtttt tccatatgca cctaatgctg
    13801 tattaattgg cttttttgtt agcttctttg taggaacact atccatgttt gctatgatag
    13861 gcttgcaaac tacagttatc attcctgggg tagtaggaca tttcttctgc ggagctgctt
    13921 ctggtgtctt cggcaactcg actggcggta gaagaggggc ggtattaggt tctgctttca
    13981 atagcttatt gattagttgg ctgccattat ttatccttcc agtattagga gatcttaaaa
    14041 tcgcttcttc tacatttgcc gatactgatt atctagtacc aggacttgta cttgggaaac
    14101 taggagaata cggtcaaata gccttgacag ttggaatcat cgctttcttt ttacttgtcg
    14161 tattatctag ctttcttttt gccggaaaaa gaacaaacat tgtttctaaa gaacagaatt
    14221 aagcaaactt caaaataaaa attgcttgga caatatcaga agaagattta aagtcttctg
    14281 atatgtcgca agcagttttt tgttttatgc atttattttt gaatagcttg ttaagacaag
    14341 tggctgtttt gctagaagag gataactttt accattcatt ttcactgttc cgcctgttct
    14401 atcagaagta atcacgatct tatcttttgt tttttcaagc gaaatctttc catctttgat
    14461 aggtagcgta caattcagtg tttcaaatga atctagatta ggttgtatca taaattcttc
    14521 ataacctggc tttgttggat acaaaccgat aaaatagcgt cctaaaagat agattgggct
    14581 tgctccccat gcatggcata agctttttcc ataaggatct ccatacatac tgtacttttc
    14641 atttcctgtt tgagtagggt cgtattcttc ccagaaagtt accgcatcat gagataacat
    14701 ccctccccaa taatcaagaa tcgtttggta gacgatattc gtctgtccga gtttgcataa
    14761 agcatcttgt tcatagaatt tgaaatatgg agtagtaatc tgtgtgatag tgtcattcaa
    14821 taagacattc tttaagatcg attgctgttg gtctttgtta gcaaaatcga ataaaacagc
    14881 gaaaatattc gcatgacgcg tcacatttct tctcccagat tcatagcaat cgataaaagc
    14941 gccaagttct tcatcccaaa agtagtcaaa taggttttgt ttcaatagtt gatatttttc
    15001 ttgatagaga tgagtatgat aattttcgtt ctctccatat gtttcagcta agatatctcc
    15061 gcaagtgatc atggaatgat aggctttcag taataagact tgttctgcag caagcgttcc
    15121 ttctttatcc atttcagacc aatcaataaa tatccaatct tttgctctcc cataaataaa
    15181 ccctaattca ttcgtttgct ccatcaagta tctcattaat gattgcatct tagggtagac
    15241 gatgcgtaaa aattcatagt ctttcgtcat catatagtgg ttttcgatac caatcaacca
    15301 caagatcgaa tagtcaacga tagtattcat atgctgcttg atttcatttt gtcctcttaa
    15361 tgctaatatc gttcgtttat tgatttcctc gtcaaaaaaa agatattgat tgatcatgaa
    15421 tgattgatac gcatctccag accaaatcca gcgatctcgt ttgataccgt cgataaaaaa
    15481 caaatcacta cataacttga aggcttcttc tgatactgac cagatctgat tcagtaaaga
    15541 agaatcgctt gaaaaagcag cttgtttcga aagagataaa aattcatgaa aaacttgaag
    15601 ttgaaccatt ccagtagaaa cctctggtat aaacaaatag cgaaaagcgc gtttacgagc
    15661 tttcattttt cttgtcattc ctatttgctt atagtagcaa taatcgatat ccatagcttc
    15721 tacatcggac tctccataac atagagtgat agattcgata tggtcatcca gagatattag
    15781 ttctacacag ccattcactg ctcgcccaaa atcgaataag attcctccgc caatctccgt
    15841 ttcagtgact ggatggacgc attctttttg ataatagatt tgatttggat cttttgcgat
    15901 atcgacataa agcggactcc agccagctgg atattcttca ataaaattac ttgctgtcca
    15961 gccaatatct gattttactt cgtctccctc gataaacatc gctggtagcc ctgatgtatg
    16021 tcctacaaag atacgaattt ttgctttacc agctggacaa gtcagccatt ttccacatgg
    16081 atgtttttgt ccattaattt ctacataccc aactccttgg atcgtcactt taaacatgga
    16141 aggttgatca agaaagtaat atctcttaaa cttgacgttc cgatggcaat catccatgta
    16201 ccagtacgct ggccagtcaa aacctcgttc ttcccgttgg aaattttgta gcagtccatg
    16261 gcggatctca aagtctccag gataccaaag ccatctatct ttatgttttt catttgtcat
    16321 tttacacgac ctccatcaaa tttattttgt tgtcaattgt tcttttattt cttcatctac
    16381 tcttatcact gtctttctat tcgtcctgtg tactactaga aggctaataa gcgcgctaat
    16441 caacagcaag atactgccat tgacgaaaac agcaagtgtt ttactcccaa gagaagcaaa
    16501 agagagaaag agcaaaccat atggtgcaat gaaagtacct aaattagcgc caaccaataa
    16561 gattgaagta ttgaaactct ccgcgtactt gggcgcttgg atacttactt ggttgaataa
    16621 gtaaggaatg aataacgaaa aagaaattcc tgttaggatc gcaccaaacc caatcacata
    16681 caggtttatc acagtggcaa tcaatacaaa gccgattccc atcagtagta aagaaagtgt
    16741 aagcatatag cgatgtgctt ttgtctgaag ccagccaaaa caaactccgg cagccatact
    16801 tgccaattgc atgattgtta agatattcgc tgcattagtt tcgtttccta gagaataagt
    16861 cgtcaggact gtggctaatt taatcgatac agcattatat accacgatga ccagcaagag
    16921 aaaaatacca taactaatca ttttaggatt cgttttgatt acttgtcttg ttggggctgt
    16981 gccatgcgat tgaagttcag ttgtttcagg tgtaggaaca aacaaaacaa acaagacaag
    17041 aataggtaag ctgatggcgt agactaaaaa ggtatattgc caaccgaact gtactagctg
    17101 tcctgcaaca aatgtcatta aagctgcacc aaatccttga aatgcacttt gaaaaccaat
    17161 catagaggct cgttcatctc cgtgataaaa ggcagcaatc atgctgactg ccaaagaatt
    17221 gatcaatcca aagccaatac ctagtccagc tcttgaagct aagacaatac tgtaactttg
    17281 tgagaagagg gggaggatac cacaaatcga tgcaatcact aatccgatga tcacagtttg
    17341 tttttgtccg attttcctag caataaaact actaatcaaa acaaaaatag tgaccataaa
    17401 agagggagca gtcgttaata attcaacagc agataaagaa cgcgtggcat aagtagctga
    17461 catactggga attgcagacg tgactgcacc agcagaagtc aaaataagag agactgacag
    17521 cagagaaaat ttcatcatct ttttattcat aagcatgttc ctttcatttt tgtaagcgct
    17581 tatcaaaaat atagtatgaa gtggttattc tctcaatagc ggaatcacag gaagttcatg
    17641 tcccaaccat agaaagtctt tatgaaacca aaaatagctt tgcatataat agcagtacag
    17701 cgtgaactaa taagcgaaag aaaccgctta caaaaaggag ggagtcaatt ggaactagct
    17761 gaattagaca ttttttttgc gtaacgaaga atcaatcgaa actctgcagc gtgaacaagg
    17821 aatgaatatc aatgatttaa acttagctta ccatcttcag acaccgccaa aaatccaccg
    17881 aggggatcta tttcaacagg gaaatattca tatcagtaaa caccgtcgat atgcggaagt
    17941 tcccgagcat acacatgatt ttgttgaaat caattatatg tatagcggta catgtttaca
    18001 aattattaat ggagaaagga tagagatgga agaaagtgac gtggtgatca ttgataaaga
    18061 agctactcaa agaatcgaat acacagggca agaggatatt ttaatcaaca tcttattaaa
    18121 agaagaaacc ttatctacag agatattgaa tcatttggca agcacaaata atctactttc
    18181 ttcttttttg ataaatgcat caaaagaaaa aggggaacat acacaattca ttcattttcg
    18241 cggcggaaaa acaagtcgtt tgacgcattt gattgaaaat atgctgttga tttattttga
    18301 tacatacagt cataagatcc gttcgttaaa tctctataca tcactattac tgatcgaatt
    18361 gactcattta ttagaacaac aaagcttttc atcagcagat ggatgcgcag aaaatgagat
    18421 gcttagagta ttagattata tcgataagca ttttacttct tgtacgttaa aagaaacagc
    18481 agctcatttt ggctacaatc cggtctatct tagtaataaa ttgaaaatgt attttgatgc
    18541 ttcctttcaa gaactggttc taaagaaaaa attcgacttt gcgttggaac tgattcaaga
    18601 aacgagtttt tcgttggaag aaatcgctaa tcgtatcgga tataaacaaa cgacttcttt
    18661 attcaaatta ttcaagaagt attcaacatg tactcccaat gaatggagaa ataaacatta
    18721 agaagaaaat ctttcactaa agcatatgaa ggaaaattgt tttaggtgcg attgctgtct
    18781 tattatatcg ttgcagcgaa tttttttgga tttattttca gttctaaact tttggttgta
    18841 ttatcagggg atataggtgt aaacatttgt catttatctg ttatgcgtat tcaggaattc
    18901 gtttccatca aaaattataa taacaactat tataattaac atgttgataa aatgaataaa
    18961 caagactata cttttcatga aagtaacaaa tagttatgaa aaggagctgt ttattcatga
    19021 aaaaaacaag tcgtaaagta gtaatcgtcg gtacaggttt tgttggaaca agtatcgctt
    19081 atgcaatgat caaccaagga atctctaatg aattagtatt gatcgatgtg aatcaagaaa
    19141 aagcagaagg ggaagcattg gaccttttag atggtatggc ttggggcgat gaaaatgtgg
    19201 ctgtatggtc aggtggctat gaagaatgta aagatgctga tatcgttgtt atcacagctg
    19261 gtatcaacca aaaacctggt caatctcgtt tagatttagt taaaacaaat gcgtcaatca
    19321 tgcgccaaat cgtaaaagaa atcatgggat caggattcga cggtattatc gttgttgctt
    19381 caaacccagt agatattttg acatatatcg cttggaacga atctggtctg ccaacatcac
    19441 gtgttatcgg tactggaaca acattggata caactcgttt ccgtaaagaa atcgcattga
    19501 aattaaaagt tgacccacgc agcgtccacg gctatatctt aggtgaacat ggtgattcag
    19561 aagttgctgc atggtcacat acaactgtcg gtggtaaacc agtgtttgaa attgtagaaa
    19621 aagaccatcg tattgcccaa gatgaattag atgtgattgc tgataaagtc cgtaatgctg
    19681 cttatgagat cattgatcgt aaaaaagcaa cttactatgg tattggtatg agtactgcac
    19741 gtatcgtaaa agctatcttg aataacgaac aagctgtatt gcctgtatct gcttatttaa
    19801 ctggtgaata cgacgaaaaa gatatcttta caggtgtgcc atcaatcgtt gacgaaaatg
    19861 gtgttcgtga agttgttgaa ttatcaatca acgaagaaga aaaagcaatg ttcagcaaat
    19921 caacaagtgc tttaagagaa gtattgaaca ctgtattata agaaacaatg aatatgaact
    19981 aaaaatccta aaactgctta acagctgaaa tagctgttaa gcagtttttt attgatatct
    20041 aataggtaag caaataaata gaatggttaa aattaatcgg tccgaaaatg tatccgtaca
    20101 tatgaacata tgaaatttaa tctgttgatt acaataagcg aatcgttatc aatgtttatc
    20161 tgtggttaat taaaaaatcc ttccgcaacg gcaaatacaa tgattcatat ctaattcgtt
    20221 catatttgat taactgaata aagaaactgg tttttatcat tcaattaaac gacaaaaaaa
    20281 gtaagaaaaa ctgcaaaatt tttttataat tttgcataag cagattttga tggaaaaatg
    20341 acgatttttg ataaaaaacc gttcgagaga ggtttatcat tttt
//
LOCUS       DACCYH010000050        20326 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.051, whole genome shotgun sequence.
ACCESSION   DACCYH010000050 DACCYH010000000
VERSION     DACCYH010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.051"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(45..1184)
                     /locus_tag="IVY85_001736"
     CDS             complement(45..1184)
                     /locus_tag="IVY85_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP8044754.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1583..2881)
                     /gene="eno"
                     /locus_tag="IVY85_001737"
     CDS             complement(1583..2881)
                     /gene="eno"
                     /locus_tag="IVY85_001737"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP8044755.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2997..3752)
                     /locus_tag="IVY85_001738"
     CDS             complement(2997..3752)
                     /locus_tag="IVY85_001738"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP8044756.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3856..5046)
                     /locus_tag="IVY85_001739"
     CDS             complement(3856..5046)
                     /locus_tag="IVY85_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP8044757.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5156..6157)
                     /gene="gap"
                     /locus_tag="IVY85_001740"
     CDS             complement(5156..6157)
                     /gene="gap"
                     /locus_tag="IVY85_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8044758.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6198..7235)
                     /locus_tag="IVY85_001741"
     CDS             complement(6198..7235)
                     /locus_tag="IVY85_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP8044759.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            complement(7438..8772)
                     /gene="rpoN"
                     /locus_tag="IVY85_001742"
     CDS             complement(7438..8772)
                     /gene="rpoN"
                     /locus_tag="IVY85_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP8044760.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8832..8903
                     /locus_tag="IVY85_001743"
     tRNA            8832..8903
                     /locus_tag="IVY85_001743"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8865..8867,aa:Arg,seq:ccg)
     gene            complement(9095..9979)
                     /locus_tag="IVY85_001744"
     CDS             complement(9095..9979)
                     /locus_tag="IVY85_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8044761.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(10066..10965)
                     /locus_tag="IVY85_001745"
     CDS             complement(10066..10965)
                     /locus_tag="IVY85_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP8044762.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(10982..11488)
                     /locus_tag="IVY85_001746"
     CDS             complement(10982..11488)
                     /locus_tag="IVY85_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP8044763.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(11677..12297)
                     /locus_tag="IVY85_001747"
     CDS             complement(11677..12297)
                     /locus_tag="IVY85_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP8044764.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(12373..12810)
                     /locus_tag="IVY85_001748"
     CDS             complement(12373..12810)
                     /locus_tag="IVY85_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP8044765.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            12973..13803
                     /locus_tag="IVY85_001749"
     CDS             12973..13803
                     /locus_tag="IVY85_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP8044766.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            13787..15154
                     /locus_tag="IVY85_001750"
     CDS             13787..15154
                     /locus_tag="IVY85_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8044767.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            15173..15982
                     /gene="yidA"
                     /locus_tag="IVY85_001751"
     CDS             15173..15982
                     /gene="yidA"
                     /locus_tag="IVY85_001751"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP8044768.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(16025..16468)
                     /locus_tag="IVY85_001752"
     CDS             complement(16025..16468)
                     /locus_tag="IVY85_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8044769.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            16602..16973
                     /locus_tag="IVY85_001753"
     CDS             16602..16973
                     /locus_tag="IVY85_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8044770.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(17142..17924)
                     /locus_tag="IVY85_001754"
     CDS             complement(17142..17924)
                     /locus_tag="IVY85_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP8044771.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(17921..18892)
                     /locus_tag="IVY85_001755"
     CDS             complement(17921..18892)
                     /locus_tag="IVY85_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP8044772.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            18984..>20326
                     /locus_tag="IVY85_001756"
     CDS             18984..>20326
                     /locus_tag="IVY85_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8044773.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEI"
BASE COUNT         5685 a         4202 c         3627 g         6812 t
ORIGIN      
        1 aataatgcta atctggctct gttttttcat ttattaaaaa aatcttacgc taagtaggat
       61 aaaataattc cttgatattt ttcgatcata tccaaataat tatcgatttc aacatactca
      121 tctacttgat gaggcaaagt aacgacacca ggcccaaaga cgacaaagtc gaaggaatgg
      181 ttagctttcg taaattcggc tgcatccgtt gttgctgccg ctccaactaa aggtaatggc
      241 tggctgaatt gttgctgaat gcaatgaatc aaaggagaat ctggatcagc tttgacaggt
      301 attttgttgt aatcgatcat caattccaaa tgatagtctg tctcttgatt caattcattg
      361 acgattgatt gcagcaatgc aatgatttta tcatttgggt attctggaat cgatcgaatg
      421 ttcccttgga gctgcgcatg gcttgggatg ctgttgactt gattcccgcc actgatcaat
      481 gtaacattat ggattgtttt tcctaggaca ggattctcga ttgtttcagc gaggtgattc
      541 atttcagcat ttgctttcgt aatgaattca tttaggtgat tgattgcatt ataaccttgg
      601 tctggcatag agctatgcgc ttcttttccg tgcgaggtga ctgtatagtt gattgatccc
      661 atgtgcgtat acatcaaact gtaattggtt ggctctccga tgatcaaggc atctaagtca
      721 tctacatagc ctgctttcgt gagctgttcc ccacctagtt cacccacttc ttctccgaca
      781 gtggctaata atttgactgt tccgttaaat ggttttcctg actctttcag ttcgatcatg
      841 gcaatcacca ttgctgccaa gccactcttc atatctgtag caccacgtcc gtacaaacgg
      901 ttcccatgga tttctgctgc aaagggtgca taggtccaag acgattcatc accagccgcc
      961 acgacatcca tatgcccaga taggcctaac acttttcctg attggccctt ttggtaacga
     1021 gcaatcaaat tatctctacc atctctgtaa gaaacaatct ctcctgttat gtcatgcttt
     1081 gccaaaagct tattcaaata ggctgctact tcaccttcgt ttccattcac tgatttaatt
     1141 cggatgatct cttgtaaaat cgcgattttt tcttcttttt tcatctaatc ctctcccttg
     1201 ccatttcttt ttattgtatt tgagtgtgct cgaattttca aataggagca accaaagtaa
     1261 aaatgaagaa agagagccac tttatgaaaa atatgctgct acttccctcc tattttattt
     1321 atacgagagg acttgctcat agattcctat tctactgttc tatccttaaa agctcttata
     1381 tgatgctagt tattgttagc tttccttttt caaggatgat cttttcttgt accattcttt
     1441 gtaaacatca tatacagaag ctagttgcaa aaaaactctc tacctgctga tatttcagca
     1501 agtagagagt tcgattactt aactaagtga tcatactcga ctatctttca atagtcgatc
     1561 atgtatcttc attagaaaag aattatttgt tttttaagtt gtagaaagat ttcaaacctt
     1621 tgtattctgc aacttcacca agttggtctt cgatacgtag taattggttg tatttagcaa
     1681 tacggtcagt acgtgataga gaaccagttt tgatttggcc agcgtttgtt gcaacagcga
     1741 tatcagagat tgttgaatct tctgtttcac cagaacggtg agatacaact gctgtgtagc
     1801 cagcttcttt agccatttcg atagcttcga aagtttctgt taatgtaccg atttggttca
     1861 ctttgataag gattgagtta gcaattcctt tttcgatacc ttcagataat ttttgagtgt
     1921 ttgttacgaa caagtcgtca ccaactaatt gaactttgtc gcctaatacg tcagttaatt
     1981 tcttgaatcc gtcccagtcg ttttcgtcta atccgtcttc gattgagatg attggatatt
     2041 tagaaactaa ttcttcgtag aatttgatca tttcgtcagt tgttttttcg ccttcgcctg
     2101 aatcagctag tacgtaaaca cctttttctt tgtcgtagaa ttctgaagaa gcagcatcca
     2161 tagcaagaac aacgtcttta ccaggtacat agccagcttt ttcgattgct tcgatgatta
     2221 cttcaaaacc ttcttcgttt gaaccaaggt ttggagcaaa tccaccttcg tcaccaacag
     2281 aagttgctaa tccgcgagct ttcaagattg aagctaatgc gtggaatact tcagcaccca
     2341 tacgtagagc ttctttgaat gttggagcgc caacaggcat gatcatgaat tcttggaagt
     2401 cgattgagtt atcagcatgt gatccgccgt tgatgatgtt catcattggt gttggcaata
     2461 cttttgtgtt gaatccgcct aggtagtggt ataaaggtac ttctaggtag tcagcagctg
     2521 cacgtgcaac agcgatagaa acacctaaaa tagcgttcgc acctaattta cctttgttag
     2581 gagttccgtc taaagcgatc atagctttgt cgatagccat ttggtcacga acatcgtagc
     2641 caatgattgc ttcagcaatg atgttgttta cgttgtcgac agctttagtt acacctttac
     2701 cgccataacg agctttgtct ccgtcacgta attcaactgc ttcgtattca ccagttgaag
     2761 cacctgaagg aaccattccg cggccaaaag ctccgctttc tgtgtatact tctacttcga
     2821 ttgttgggtt accacgtgag tctaagatct cgcgagcgta aacatcagta ataattgaca
     2881 tgttttgtct ctcctttgag tttgtttttt cctaagggat ttccctcata tacgatttta
     2941 gttaattttg ctcattagtg caaacatttc ttgcattttt cgcaaatttt caacaattat
     3001 tttacagcgt ctaacaatga caagaatgaa tctgcttgca agcttgcgcc accgactaaa
     3061 gcaccgtcaa cattttcttt tgccatgtat tcagcaatat tttctggttt tacagaaccg
     3121 ccgtattgga tacgaactgc ttctgatact tcttttccat aaagtttttc aactgtttga
     3181 cgtacaactc cacaaatttc gtcagcgatt tgtgcgtcag ctgatttacc agtaccgatt
     3241 gcccagattg gttcataagc aataaccatg tttgacacct gttcagctga aagatcagct
     3301 aaaccagctg taatttgtcc ttcgatccat tcagcagttt tgcctgcttc ataggtttct
     3361 aaagattcgc cacagcatag gattggtgtc ataccatttg caaagatagc tttcgctttt
     3421 ttattgatgt cttcgtctgt ttcatggaaa tattcacgac gttctgaatg tccgatgatc
     3481 acataatgaa cacctagatc agcaagagct gctggtgaag tttcaccagt gaacgcacct
     3541 gagttttccc agtagcagtt ttgtgctgag atttttagtt ctgtaccttc agctgatgtc
     3601 attaattctt gtaagaataa tgcaggagat ccgatgactg aatcaacttt gtcatttgaa
     3661 gggattttgt tttttactgc ttcagcaaat tcttttgctt ctgatgcagt tttgttcatt
     3721 ttccagttac ctgcgataat aggtttacgc attgaggaac acactcctta atttattgaa
     3781 aaatggctag ggagtcatga aaactccctt caccttttgt tcgattactc gtcagaattg
     3841 agcaaacttg gtcgtttatt tatcgttgat tgctgctagt cctggtaatt ctttaccttc
     3901 aagcaattcc aagcttgcgc cgccacctgt tgagatgtgt gtgaatttgt cagcaaatcc
     3961 taattgttct gctgctgcgg ctgagtctcc gccaccgatg attgttgttg cgccttctaa
     4021 gttagcgatt gcttcacaaa caccgattgt acctttagca aagttactca tttcaaatac
     4081 acccataggt ccgttccaaa caactgtttt tgcaccttgt aatgtatcag caaataattt
     4141 tactgatgct gggccgatgt ctaaagccat caaaccatca ggaatgtctc cttcgattac
     4201 ttgtgtttca acatcatttg aaaattctgg tgcacagaca ttgtcgattg gcaagacaag
     4261 tttgtcgcct gctttttcga ttaattcttt tgctagttcc actttatcag cttctactag
     4321 agagttaccg atcttgattc cttttgcttc gtagaatgtg taagtcattc cgccaccgat
     4381 caagatttta tctgctttag gaatcaagtt ttcgattacg ccgattttat cagaaacttt
     4441 tgcgccacca aggattgcaa tcattggtcg ttttggttct tctactgctt caccgatgaa
     4501 cttgatttct ttttccatca agaatccagc aactgttggg ataccagttg aagcgatacc
     4561 tacgttagaa gcatgtgcac ggtgagctgt accgaaagca tcgttaacaa atacatcgcc
     4621 taatgaagcc cagtatttac ctagttctgt atcatttttg ctttcttttt taccgtcaat
     4681 atcttcaaat cgtgtatttt cgaatacgac aacgtcgccg tcattcatgt tgcggattgc
     4741 ttcttctaat tgttctccgc gagtttcagg tacgaatgtt acttctttac ctaataattc
     4801 gcctagacgt tttgcaactg gagctaaaga ttttccttct ttatcttctt ctgtttttac
     4861 acgtccaagg tgagagaaaa ggattgcttt tccgccttgt tctagaacgt agttgattgt
     4921 tggcaatgct gctttgatac gagtgtcgtc agtgatcacg ccgtctttca aaggaacgtt
     4981 gaagtcaaca cggacaagaa cttttttgtc ttttaaatcg atatctttca ctgttttctt
     5041 agccatttta tttcctcctg aataaactta ttatcaaaaa aagcggaaga agcgcgattg
     5101 cttccccgct tttaaatgta catgtctata atttacactt gtacgtttgt ctatcttata
     5161 agttagcgaa gtattctaaa gtacgtacta attgtgcagt gtatgacatt tcgttgtcgt
     5221 accaagcaac agttttaact aattgtttgt cgccaactgt cattacttta gtttgtgtag
     5281 catcaaataa tgaaccgtaa gtcataccaa cgatatctga agaaacgatt tcgtctgtgt
     5341 tgtatccgta agattcgttt gaagcttctt tcattgctgc attgatttcg tcaacagtta
     5401 cttctttttc aagaactgtt actaattctg ttaatgaacc tgttggtaca ggaacacgtt
     5461 gagcagctcc gtctaattta ccgttcaatt caggaattac taaaccgata gctttagcag
     5521 cacctgttga gttaggaacg atgtttgcag cagcagcgcg tgcacggcgg aagtcacctt
     5581 taggatgagg tccgtctaga gtcatttggt cacctgtgta agcgtggata gttgtcatta
     5641 atccttcaac aacaccaaat ttgtcgttca atgttttagc cataggagct aaacagttag
     5701 tagtacatga agcacctgag ataactgttt ctttaccagt taatgtttcg tggtttgtgt
     5761 tataaacgat tgttggaaca tcgtttccgc caggagcaga gataacaacg cgtttagcac
     5821 cagctgttaa atgtttttca gctgctgatt tagaagtgaa gaaaccagta cattctagaa
     5881 cgatatctac gcctagttcg ccccatggta attcttctgg gttgcggtta gctaatactt
     5941 taacttcttt tccgtttacg ttgaatgaac cttcatgaac ttcaactgtt ccgttgaaac
     6001 gtccttgagt tgtgtcgtat tttaacaagt gtgccaacat tttagcatct gtcaagtcgt
     6061 tgattgctac tacttcgatt ccatctacat cttggatacg acggaatgct agacgtccaa
     6121 tacgtccaaa accattaata cctactttaa ctgtcattta agatttcctc cttatgaaaa
     6181 tcaaaaaaat atttatttta aagggttacc ccttttaaaa tctcatttgc agcagcttca
     6241 tcagtgatga gccacgtttg ttttggtgca tttttcatat aagctcgaat tgctttagct
     6301 tttgattttc cgcccgcgat tgccatgatg atcgggactt tttccaaatc ttgcaattct
     6361 atgccgattc tagggacctt ataaacgact tcaccttttt cattgaaaaa gtagccaaaa
     6421 gattccgcaa cagcatttgc ttgtttcagc atgactaatt ctttttcagt catcttacga
     6481 cgtgctgcca tgtgtaatgc acgcccaata ctatgaataa cacaattcgc ttcactaatc
     6541 aagtttaaga cttcttggat agatggctca ttgagtaaag aattgtaagt tgccagactt
     6601 aattgttccg gaacataaag tgcacggtgg ttaccgtttg ctttagcagc cattcttgca
     6661 ctgacagaat ttgcctgtac agttatcgct tcaccaatcc cgcctcttgc agggacaaac
     6721 aggttatgcc gtttcttatt ttccagatta gacaattgtt cggctaccac agccatagta
     6781 gttcctccca tcacagcgat gatattctcg ccttctggaa gtagtcggtc tagggcttcg
     6841 ctcagcgctt cgccgaatgc atcagccact ttggtctgct cttcgctgtt tcctgaaaca
     6901 acgatacagc gttggttacc aaaatattct gccaactgtt gttcggtttg atgcgtacct
     6961 aaaagctgat ccatgaagtt ttcaaggctt tgataaactt cttcgccctt agttgtcagc
     7021 gtcataccgc tttttgaggt gtcgatcaaa ttcagatttt tcaaaagatc tgtttctgtt
     7081 cgcaatactc gttctgtgat ccccatactt tccgataaac ttcgacgtcc aatcggctgc
     7141 atccaataga tatttcgtaa aattttatag cgttcttgca acacttcgat aatatctggt
     7201 gcaacagatt caatcatttt tatttcatcc agcatagcga tctccttagt tggacacttt
     7261 acgtccaatg tagacttaaa acgacccata tacgataaaa aaagaaatta actattcgaa
     7321 tttcacatca ataaagggaa atctcaacct cacgactgta agtctaacag ttttgctctc
     7381 gtttttcaac taaaaagact agaaaaagtg aaatactttt tctagtcttt tgactgatta
     7441 gttatcataa cgttttcttt ttgatgaact cgggattcct aataaatcac gatattttgc
     7501 tactgttcgt cttgagatcg ctatctcatc ttttttcagc aattcaacca atttctggtc
     7561 agacaacggc ttgtttttgt cttcttgatc gactaattcc tgcagctttt tcttcgctgt
     7621 atctgctgaa aggtcttctg tctgttctgt actattcgta ggaattcgtg tagtgaaaaa
     7681 tttttttaat tcaaatacac cgaagtctgt ttccaaatat ttcccgttga ctgcacgact
     7741 gaccgtggat tcatgcacat ctatctcagt tgcaacttcc tttaacgtaa gaggcttgat
     7801 tggacgttcc ttgtcgataa agaaagcttt ttgccgggaa acaatgactt gagcaacccg
     7861 caaaattgta tccccacgtt gtaggatcgt tttcttcaac cattcgaatt cctgtttccg
     7921 ttcttttaaa taattttcag tttctttatc tgcttgttgt gacatttgtt tgaagtaact
     7981 ctcttgaaag cgtatatttg gctgattttt tcggtttgag ataacttgta catttttatc
     8041 gtcgtccact aatacccgga catcaggaat aatgtacaat tcgctgctac ggtcgaaaat
     8101 ccggccaggg cttggactca acgtttgtaa atagtcgaat atctgctgaa cagcatgtag
     8161 atcaacagaa aaacgttgtg cgatttcttt ccattttcgc tccaccagtt cctcaaaaca
     8221 ttcttccaac acgatataag ctaattccgg cgcataatca tctcgttctg tttgaagcat
     8281 gagacattct tgcaaactac gtgcacctac tccagctggt tctaattgct gaatcagagt
     8341 caacgcatcg agcatttgga taggcgtagc tccaatctct tttccggctt cttctaaact
     8401 aatcgtcaaa aatccgttca aatcgatgta ttctacaaga taaagaacga ttttcctcaa
     8461 aaaagtatct cggtaattca aatggacttg ttcgatcaat gaatcaaata aagacccttt
     8521 tgtatcagga atctgattta ggtaatttgt ctcttctcct tcataagaag atgaagaagc
     8581 ttttatgtaa tctggctgcc attccggctc aacaacttcc accaatggat tttccattgc
     8641 ttgattctca acgaatgcca acagctcctc actgttatat tgtaaaactt ggatggactg
     8701 ttgcaactgt tgcgtcatgg ctagtttctg tgtctgctgc tgtttttgtg aaaaattttg
     8761 ctcaaatttc atttttttgt ccaatcccct tgtaattttt tatctgttta gttacaatta
     8821 tcccagtgct tgcgtcctta gtgtagtgga tatcacgtaa gattccggtt cttgagacgg
     8881 gggttcgatt ccctcaggac gcgttctact gctattttag cataatttac gaatagttgc
     8941 ttctttattt ttaagtgaaa actgtccgcc aaagaaaatt ggtggacagt tttttgttgt
     9001 acctgtatag atattgttaa gcctttgcaa gtaaggtagt tatgtgttca actacttatt
     9061 aaaatgtgtt atttattaaa gatacttgtc tcacttactg aactggccga tgatcgtttc
     9121 tgccataata gttctcgaca gccgttggat gccctaataa atccccaact gtcgtgatgc
     9181 gatacccttg ctctttcaga taatcgatca cttgaggaac ggcacgaatc gtttcaggat
     9241 agatatcatg gaataaaata attgccccat cgtatactgt tgcttggatc ttctgcaaaa
     9301 tcaaatcagg atttcttgtc ttccaatctt ctgaatcaac agaccattcg atgatcggtc
     9361 gctggatgat cgttgctcct acattcgtga cagaaccata gggcggacga acaaaagtgg
     9421 gggttttgcc agtgactttt ttgatctcag tgtctgcttt cgtcacttcc tccgtcatcg
     9481 actgggcaga aagagtggcc agattctgat gatcgtatgt gtgattgcca atctcgtgcc
     9541 ctgcttcagc tgtctgcttc acgatttcag gatgctgcgc cgtattttct cctaagacga
     9601 aaaaagtggc atgcacgttt ttttctttta agattcgcag aagctgcggt gttgtggtcg
     9661 ctcctggtcc atcatcaaag ctgattgcga cgcttttgac agccggatct cggctcgttt
     9721 ttgtactgtc ttgtgttgat tgtgatgact gactttgtac cttttccgct gattcttgaa
     9781 tggcttcttt gacgttagct gtttcagttg attggctttt aatggttgat tgttctgatt
     9841 gtttgaaaga ttgtgacgaa tgctccgaag agtgtgtttg tccttttact tcggtgatca
     9901 aggaggccac cgaagtaatt tcgaaaaaca aaagcacgga taaagcaata atactgataa
     9961 ctaaataatt tcttttcact atatctcttc tttctctttt tctcttctta ttatttactt
    10021 gtacttactt gaaaattgaa actgctagtc gcaactactt ctcgtctaat gatccaaata
    10081 atcttccgat gccaaaatcg tttttgtcac ggacttagga atgacaaatt ctggtgtacc
    10141 catataacca ggagcaattt cgtattgaga aaaagtcaaa actaattgat gctctttgtt
    10201 caaataaaaa cttcgtgtag gcgtaacaag tgttggtttt tctatcgtcc cttcttgaat
    10261 atcttgctca gtccaatagt acttagatgg atcttccttg acttgttcgg ccatctgccg
    10321 tttgacttct tttgtcaaaa cagatagata ggtgtcatct ttgaataaaa gtggaagtga
    10381 gagctggaca ctgtttttct tgtcgattgt gtcgtatctt ttttcaacat ggctatctgc
    10441 tgctgtttgg gtgatctgcc tttcaatcac gagaaaccgg ttatcgtcta cgattttctg
    10501 ataatctccc aaaacttggt aatgatccgt ttcaaaattt ccatattctt tttcagcttt
    10561 ttcaaaatct gcttgtcctt cgcgaaagta tttttgattt aatccatgaa ttgttttatt
    10621 ttctttctca ttttgtgtat tgatcttcgg tacctgaata tcgatttttt cagatcgatc
    10681 agtaaagcgt tcgcctaaga tcaattcaat gactgatcct aagatgggta aatcttctgc
    10741 aaagcttcga acttgactat taaaaagaag gctacttgtt gtaatcatta cgatagccaa
    10801 agatactccg aacccttgga gacgtctctt tctccgcttt gcttttataa atgattgctc
    10861 ttcgataata aaacgctggc ggatctcctc tttaagccct ttgggaattg cttgttcttt
    10921 ataagttttc tttagttttt ttaaattttc tttttgggaa tacatggcac tcttctctcc
    10981 ttcattctag gatttgacgt aaagcattca atgagcggta cattttcgtt ttaactgtgt
    11041 tttcattcat atccaagata gtcgcaattt ctttcaaacg aaatccttca aaaaagtaga
    11101 ggatcagaat ctctttttcc ggactgtcta ttttatctaa aatcatatgg agctctgcaa
    11161 cggaagtttc taaaggtaac tgttggggga tatcttgaat ttcaaataag tccacaggcg
    11221 tccttttttg tctgcgccaa ctatctaatg ccgtgcggat caatatccga taaaaccatg
    11281 cactaaaatg ctcgatctct ttttcttttt caaaatagtt cagggcttta tgaaaactat
    11341 cttgcatcac atctaaggcc tcttctttat catgtacgta attcatcgtg atacggaaca
    11401 ttttttgata atcagtttcg atgagtccat tcagcaggtg acggtatttt tcgatctgcc
    11461 gttttttctt atatccagta aaaatcattc tccactcctt tctgcctcac ttaatatgac
    11521 gtacaaggtg cgctaaaagt ttcaattttt ttatttttta tgtatttact tgggattttt
    11581 atatacaaaa aagaaggtgt gacaaaaatc gtgtcacacc tccttattct gataggctga
    11641 tcgtctctgt cacaacctct cgtaagaaaa taatgtttat tctttttcag agtcagaaaa
    11701 ttcttcctct tcataatctt catcatcatc gtcttcgtct atgatactta aatcttcttc
    11761 gatatttcct ggcaagtctt cttcatcatc tgtcgggtca tctgctccga tttcttgcaa
    11821 gtcagaatta taagcagcga tttcttcatc ttcatcatca tcgtcatcaa acagatcatc
    11881 gtcatcttct tctgtcagat ctgtatcttc tgggtcatcg tcattgtaat cgatcgcgtc
    11941 ttcatcatca ttgataaagg cgttgacttt cttgcgttta cggcgacgtg gagtatcttc
    12001 atcctcttcg tccattccat ggtttacttc ttcatcgatc gaatcgatag cataccatga
    12061 acgcaagccc caacgattat cccctaatga gataaagctg ccgtcaatat tcaagtcagt
    12121 ataaaattgt gcgagtgaat cacgaatttc actgtctgat ttcccaagat agttttggat
    12181 ctggttagct aaatcagaaa aatccatgac atcgccacgt tgttctaaga tcgcgtgtgc
    12241 tacttcgatc attgataatt cgcttttgtt tgcaccttca aatacactaa tttccaatca
    12301 ggacacgtcc tttcacagtc tactccttat catacaaaat cagacacaaa aaatcaaact
    12361 tttcttttat atttacgctg taaaatccaa ggtcaaataa tattcgccga ttttttccgg
    12421 tcccatgaac agatcataat cgattcgaac cttccctgct gtgggacggt ctttcaaact
    12481 aacatgcagt tctttcgtaa aagtagcaat ctggatcata ccgtatggtg ttcgatagga
    12541 tgtttctaac cgttctttat agccaaattt caaacgcatc cgcatttctc ctgcacggat
    12601 cagctgtaca tgaccatctg gaactaattt gatcgttaca ggaatctcat cgcccgtctc
    12661 ctcctgaatc tctttataac gtatgtacag cgtatcgcca attgtcacca tttgcccttt
    12721 caaatcaaaa taaaaatctt gcacttcccc ttgctgtttt acttttgtac gcaatttgat
    12781 tgaaatcggt gttccttgtg ataattccat tattcattct cctttacttc tcatagctac
    12841 attgttccta gtttcttttg ttttgtcaac caacttgtcc atttttacag aaaacctttt
    12901 ttccaaaaca actctttgat tttagagtat aatattgtag aaaatatgaa tttatacaaa
    12961 ggagccggtt gaatgaaccc tgcgattcga ctagatcagc acatttatca aaaaaacgac
    13021 ttttcccctt ttgcattaac agatatatta acggaatatt ctaaagaaca ccagcaaatc
    13081 tttttacatt tctggcaata tgactgcaca gtcatcttag gtatgaagga tgttcgaact
    13141 ccttttctaa gtgacgggat ccaaacactg aaagatgcag gttacgctcc agtgatcaga
    13201 aattcaggtg gattagggat cgtatcagat gaaggaattt tgaatatttc tttgattttc
    13261 ccccaaagtg acacaaaaaa aataaccata gatgacgctt acgagcaaat gctcctattg
    13321 actaaatacg cttttccaaa ggcaccgata caagcattcg aaatcaccca ttcttattgt
    13381 cctggaactt atgatttaag catccatggc aaaaaatttg ccggtatcgc tcagcgtcgg
    13441 atcaaaaatg ggatttctgt tatgatgtac ttaagtgtca acggtaatca ggaaaaacgc
    13501 gggcaagtca tgaagcgatt ttatcaatct gctttaaaag aagactttgg tacaaatggc
    13561 tatcctgcag tggatcctgc tacaatggca acacttgaag aactgcttga ccaacccctt
    13621 tttgtaaaag aagtcaaaga agcatttgca aaagcttttg aaaagctgta ttctgcaagt
    13681 gatttacagg attctaccga ctggctaaaa aaagaaatcc agctggagca atggaacaac
    13741 caaatccaga gaatggaaca acgtaataac ataaaggagc tacattatga caacccctta
    13801 taaatatcaa atgctgccta tggaaaaagt ttttcgtgat ccagtccata actatatcca
    13861 tgtccagcat caagtaatct tagatttgat caattcgaaa gaagtccaac gtctacgacg
    13921 cattaaacaa ttaggcactt cttctttcac ttttcacgga gcagaacata gccgttttac
    13981 tcattcatta ggcgtttatg agatttcaag acgaatctgc gatatctttt ctcgaaattt
    14041 ttcaaaagaa aaaataggca acggaggctg ggatgatcag gaacggctgg tgactttatg
    14101 tgcggcactg ttgcatgatg tcggtcatgg tccttactct catacatttg aacatatctt
    14161 ccatacgaat catgaagcca tcactgtaga gatcatcact tctccagaaa cagaagtgta
    14221 tcagattctt aaccgagtag aagaaggttt tcctgaaaag gtagctagtg tgatccaaaa
    14281 aacttacccc aacccacaag ttgtccaaat gatttctagt cagatcgatg cagatcgaat
    14341 ggactatttg cttcgggatg cttatttcac tggaacagaa tatggaacat ttgatcttac
    14401 ccgaatatta cgtgtgattc gtccttatga aggcgggatc gctttttcca tgagtggcat
    14461 gcatgcagtg gaagattata tcgttagccg atatcaaatg tatgtgcaag tgtacttcca
    14521 tcctgtttcc cgtggtatgg aagtaatttt ggagcatctg cttcatcgcg cacacgagtt
    14581 gtatcaagaa gatgccagca gttttgaact ccattctcaa ttacttctgc catttcttga
    14641 cggaagcttt actttagaag aatacctcaa acttgatgat ggggtcttgg gcacttactt
    14701 tactcaatgg tctgaatctg ctgatcctat tttaaatgat ttagctaaac gttttttgaa
    14761 tcgtaaacca ctgaaatctg ctactttttc tggcaaccgg gattcaaaat tagtccaaga
    14821 actcactttg ttagtagaaa aggtcggata taatcctgtt tattacactg ctgtgaattc
    14881 tagctacgat ctaccttatg atttttatcg cccagaacaa ggcagacatc gcacacaaat
    14941 cgagattttg cgaaatgacg gaactttgat agaattatca caggttagtc agcttgttgc
    15001 tgcattagcg ggacaagaac aaggagatga gcgatttttc tttccaaaag aaatggttga
    15061 tccgtccttg cgtgatcatt atgatttatt tgacgaaact tatcaagaat ttgccagcca
    15121 tatccgtaat ggcgcattga ttgaaataaa ttaaaatata ttggaggaaa ttatgtctat
    15181 caaactagtc gctatcgaca tcgatggtac tttattaaat gagaaaagag aagtaacaaa
    15241 agaagtaaaa gaagcgattg ccgctgctgt tgcaaaaggt gtttcgatcg ttttatgcac
    15301 tggccgtccc ttaccaggtg tccaggaaca gttaaacgag ttgaaccttt ttcaagataa
    15361 tgattacgtt attacttata acggggcact cgtacaacaa acgaaatcag gtaaaatcat
    15421 cgcccgtcac ggactgacac atgaagattt cctagagatc gaagtgatgg cacgccgtgt
    15481 cggcagtcat ctccattcga tcgatgacaa agcgatctac acgcctaatc gagatatcag
    15541 cgcttatacg atccacgaag caagtttagt caagatgccg ctaaaatatc gaacacctga
    15601 agaaatgaca ccagacatga acatcgtcaa aatgatgatg atcgacgaac cagaaatttt
    15661 agatgcagca attgcgcgtt taccgcaaac tttccgtgat aaatacacaa cggtcaaaag
    15721 tgcgccctat tacttcgaag tactaaataa agaagcaagt aaaggtgcgg cagttgcgaa
    15781 tctcgcacag caccttggta ttgatcaaga tgaaatcatg gctatcggag ataacgagaa
    15841 cgacttatcc atgattgaat atgctggtct aggagtagca atgggaaatg ccgtttctct
    15901 tgtcaaagaa gcagcaaatg tcatcacttc atcaaacgac gagcacggtg tagctgaagc
    15961 aatcaaaaaa tatgtactgt aataaataaa aaaactgaga gaaagctgat ctactcgctt
    16021 tctttcagtt tttatttttt tcgatccatc tctcgactgc ccatcggtgg ctaccacgtt
    16081 tcaatttcac aagcgcttct tgagggctca cccaccaaat tttattggtc ttttcagttg
    16141 gttctcctat acgcttccaa gaatcagcaa caaagaaata tcctggatta taataataag
    16201 ttgcacgata attcgagtaa aaatactcat cagcttctcc cagataagaa gaaatttctg
    16261 ccacaattcc catttcttct atcagctcac ggacaatcgc ttcttctttc gtctcttctc
    16321 cttcaatttc cccgccagga agaaaaaaag caccatttgg agcctcgatt actgcaatct
    16381 gctgatctct ttctatgaca atatatgctg cataacgggt ttgataatca gcaaattgct
    16441 ttttttcacc aaaaattggt ttatccatga atagtttcca ccttaacagt tttattttct
    16501 atccatttta catgagaaat ttctcatgaa caagcacaaa tcaactattt ccttacttct
    16561 ttttgttatg attgagtaaa aggaaaagga gagatgaatc tatgaaaatg gctcacacat
    16621 gtgttcgagt gaaagatctt gaagcatcaa tcgaatttta tcaaaaggca tttggttttg
    16681 aagaaagccg ccgccgtgat tttccagaaa acaaatttac acttgtttat ttgacacttc
    16741 ctggggatga ctatgaatta gagttgactt acaactatga tcatggtgcg tatgatctag
    16801 gagacggcta tggtcatatc gctatagcag ctgatgatct agaaaaatta cacgagcaac
    16861 ataaagctgc tggtcttgag attactgact taaaaggatt acctggcact gcgccttctt
    16921 actactttgt cattgaccct gatggttata aaatcgaagt catccgagga tagttagtat
    16981 ggggcatttt actcttgatt gaataaaaaa caaacaccaa taattaaaat gagacgcaaa
    17041 cttttcatta aattgaaaaa gtttgcgtct ttttttaaca accattgtag ctataaggtt
    17101 gccactctcc accaatttat ttttaattca tttgaaagaa atcaaataaa cgaggtacct
    17161 ttagaaaaca aaactgcttt tcctcctatt atcacacgat cttcttttaa ttcacattct
    17221 agatccccgc cccttgatga catttgataa gctgacaatt ttgttttatt cattctttct
    17281 gcccaataag gaataagatt agaatgtgct gatccacata ctggatcttc attgattgtt
    17341 aattttggaa agaaggttct agaaacaaag tctttttgca cactttctgc tgtcacgatg
    17401 actcctattc ctaattcaaa atttttgata gctgtaaaat ctggctgcaa tttagccacc
    17461 gttttttcat cttctaatac gaaaaataaa tcacgtgcta agtaggcttc tttaatctta
    17521 gccccaatcg cttcttcata ttcagacaat atcggcactt ttttcggcat aatactagga
    17581 aaatccatgt aataataatc tttaattctt gtcacaaata gattaccgct ttgagtagaa
    17641 aatttaatcg tttcatcagg tattttgtaa tagttaaata aaacaaatgc tgttgctaat
    17701 gtcgcgtgac cacaaagatc gatttctcga tctggcgtaa accatctaag ctcaaattct
    17761 tggtttttct tcactgtaaa tgctgtttca gataaattgt tttcaatggc aattttttgc
    17821 atcgtacctt ctggcagcca ttcttctagc acataaaccg ctgctggatt tcctttgaac
    17881 acctcatctg caaatgcatc aactacgtaa taatctaagt tcattttaaa gcccctttct
    17941 ctaatttgtt ataaatatat tctgcacaac aaagatcttc aatagctaga ccaactgcgt
    18001 cgaaaatcgt cggcctcgat tctcctttat aaaatgtttt tttctcagaa actaattcgc
    18061 ctaatgtacc aacaatacaa gattcctcta aatctccttc tgccatggct ataagcaggt
    18121 ccccgctttc tgttaaggca gctgagtaat catctacata gatttgagaa gtttccatca
    18181 acgatgtact tatctctctt gtatcaggag taaatgtgcc cacagcattg atatgtgcgt
    18241 cttcccttac ccactctttt tgaagaaatg cctctttact agaggtcaac gtacaaatga
    18301 tttccgctgg acgtactgca tcttctactg atattgcatt tataaacgtt attttaggat
    18361 actgtttttt agctttagcg ataaaagtat ttcgtctttc ttctaaaata tcgtaaacaa
    18421 acactgtagt gatgggacga atggtagaaa tcgcagtcaa atgcgacaat gcttgttgac
    18481 cagctccaat taaagcaagt gtttctgcat gcggatttgc taagtaatct gttgccagcg
    18541 cagatacagc agctgttcga atccaggtga ctgcatttgc gtttatcaat gctttgggta
    18601 gtccgttttg agcatcaaac aagaaaatct ctcctaaatg agaaggtaaa tgatactgat
    18661 gattatccgg aaaagcggta atgacttttg tgccaaaata tctgttctcc cccaaataag
    18721 caggcatcat cgcaaacata tttttgcctt ttccgtcagg taaaatttcc accaatcgag
    18781 gattttggct gattttacca ctttcataat caaaaaaaca ttttttcatt acttgaatcg
    18841 cttcttcaaa actaaaagaa gctactactt cctcaaaatt aataattttc atagacaatt
    18901 ccctcctcac aaaagattaa cttctaaaat caatacaatc aatggtaaaa ttgtatggta
    18961 tacaatttta aggagtggag aaaatgccta ttaattctta tgacaattac ccgttaactt
    19021 ggcgaccaga caaacaaaaa ttgaagcgtc cttattatcg atcgttgatt caacaattag
    19081 aacaagatat tttaaagaat aaattacaga aaaacactca gttaccctcc caaagagagc
    19141 tagctgatta tttagattta aattttacta ccgttggcaa ggcttataaa tatggaattg
    19201 agaaaggttt gctgtatacg aatattggaa gtggcacatt tatttctccc aatgtctttg
    19261 agtccatcac catctctacc aatgaagtaa ccgaacatat cattgatttt ggtctggtta
    19321 gttcttttga agaatgtaat aaattcgttg tcccgtttat tcaatcaact agtaataacc
    19381 caaatcttgt tggtttgctt aattaccaag atcccaaagg cacaaatttt caattgacta
    19441 ctgcagcaaa ttggttaaaa actcaaggag tcattccgtc cttagaatct ctatctattg
    19501 tttccggtgt tcaaaatgga ttggctatca cactttctgc acttttctct ccaggtgata
    19561 gaatagcaac agaccgatat acatacgcta atttcattga attggctcag ttatatcatt
    19621 tagagatcgt tccaattgat tttgacgagc aaggaatgcg acctgatata ttgattcagg
    19681 aatgtagaaa aaagaagatt catggtgtct ttcttatgcc ttcttgtaat aatcctatag
    19741 ggtttcaaat ctctcacaaa agacgagagc aattagccga agtgattgaa aaagaaggtc
    19801 tatgggttat tgaagatgat atccattcat ttttaacaac ttactatcaa catacgatca
    19861 ttcctccttt ccaaaattta ttacctaacc aaacaatcta cttagctgga atgacaaaat
    19921 ttatttgttc tggattgcga gttgcttatc ttgtagttcc agtacatgtc caaagcaaga
    19981 tagaacaagc aatttttaat ataaatgtga aaacttctgg attagatgtg gaagtcatca
    20041 ctcaaatcct ccattcaaat gaagcaaata ggatcttaga aactaaatta agtcttacaa
    20101 aaagagccaa tcagcttttt gattctcttt tcaaagtaga aaaaccaacg aatcgtgtcc
    20161 cttactatcg aattcttcca gttgatcctg agattgaccc taagataatt gagaatgact
    20221 ttcttgctaa cggtatccga ctttatcatt ccagacgctt tactacacgt acgcaaccag
    20281 atgcattcgt tcgaatttcc ctatcctcta ataaccttga aatttt
//
LOCUS       DACCYH010000051        20007 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.052, whole genome shotgun sequence.
ACCESSION   DACCYH010000051 DACCYH010000000
VERSION     DACCYH010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.052"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            40..852
                     /locus_tag="IVY85_001757"
     CDS             40..852
                     /locus_tag="IVY85_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP8044774.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            849..1649
                     /locus_tag="IVY85_001758"
     CDS             849..1649
                     /locus_tag="IVY85_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP8044775.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1810..3078
                     /gene="rseP"
                     /locus_tag="IVY85_001759"
     CDS             1810..3078
                     /gene="rseP"
                     /locus_tag="IVY85_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP8044776.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3146..4855
                     /locus_tag="IVY85_001760"
     CDS             3146..4855
                     /locus_tag="IVY85_001760"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP8044777.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5067..9425
                     /locus_tag="IVY85_001761"
     CDS             5067..9425
                     /locus_tag="IVY85_001761"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP8044778.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9570..10043
                     /gene="rimP"
                     /locus_tag="IVY85_001762"
     CDS             9570..10043
                     /gene="rimP"
                     /locus_tag="IVY85_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP8044779.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10066..11241
                     /gene="nusA"
                     /locus_tag="IVY85_001763"
     CDS             10066..11241
                     /gene="nusA"
                     /locus_tag="IVY85_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP8044780.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11263..11556
                     /locus_tag="IVY85_001764"
     CDS             11263..11556
                     /locus_tag="IVY85_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP8044781.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11553..11864
                     /locus_tag="IVY85_001765"
     CDS             11553..11864
                     /locus_tag="IVY85_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP8044782.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11877..14183
                     /gene="infB"
                     /locus_tag="IVY85_001766"
     CDS             11877..14183
                     /gene="infB"
                     /locus_tag="IVY85_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP8044783.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14210..14557
                     /gene="rbfA"
                     /locus_tag="IVY85_001767"
     CDS             14210..14557
                     /gene="rbfA"
                     /locus_tag="IVY85_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP8044784.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14714..14887)
                     /locus_tag="IVY85_001768"
     CDS             complement(14714..14887)
                     /locus_tag="IVY85_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8044785.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            15335..17697
                     /locus_tag="IVY85_001769"
                     /pseudo
     CDS             15335..17697
                     /locus_tag="IVY85_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18065..18988
                     /gene="truB"
                     /locus_tag="IVY85_001770"
     CDS             18065..18988
                     /gene="truB"
                     /locus_tag="IVY85_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP8044786.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18992..19933
                     /gene="ribF"
                     /locus_tag="IVY85_001771"
     CDS             18992..19933
                     /gene="ribF"
                     /locus_tag="IVY85_001771"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP8044787.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
BASE COUNT         6858 a         3519 c         4393 g         5237 t
ORIGIN      
        1 taagatagtg ggaatctttt aagtatagga ggaaaaagaa tgttacgttt ttttccgcaa
       61 aaaaacaaat atattcaaga aaaaagcgaa tttcaattta ataccgatgg accaattcct
      121 cagcacattg ccatcatcat ggacggcaat ggtcgttggg cgcaaaaccg tcgtttgcca
      181 cgagttgctg gccataaaga aggcatggaa acagtaaaga aagtcacaaa aaaagcatca
      241 aggcttggag tgaaagtgtt gacgctttat gccttttcga ctgaaaactg gaagcgacca
      301 aaagatgaag taagttttct catgcaacta cctgtcgact tctttgatac gttcgtacca
      361 gaattgatta aagaaaatgt caaagttcat gtcatgggtt atgaaaacgt attgccagaa
      421 catacgcaag atgctgtaag acgtgcaatt gagcaaacaa aaaacaatac tggaatggta
      481 ttgaattttg ctttgaatta tggaagcaga gcagagatcg tgactgctgt ccaagaaatt
      541 gccgaagaag tagcaaaagg agaaatacac gcagaagaaa tcgatgatga attgattgct
      601 aagcatttaa tgacaggttt cttgcctaaa gagttacaag atcctgagct gatgatccgt
      661 acaagtggag aagagcgaat tagtaatttt ctgttatggc agatcgcata tagtgagctg
      721 tattttacaa aagcgctttg gcctgatttt gatggtgcgc atcttgaaga agcaatcgcc
      781 tcttatcaaa atcgagaccg tcgattcggc ggtgtaaaaa aaacagaaga tgagaaaggg
      841 gatcagtcat gaaacaacgt gtggtcacag cagccgtggc gctagtcttg tttattccga
      901 ttatatggat cggaggcata gcggttgagc ttacagcagc aatcttagca gtaataggag
      961 tttacgagct gtttcggatg aaaggattga cactcttaag ttttgaagga gttttatccg
     1021 ccgttggtgc agtgttactt gttttgccaa aagaaagatg gtttttcttt cttccagaga
     1081 atgtaagtac atttatgtta ttttatctta tagttatgat tcttttagga acatcagtta
     1141 tttctaaaaa tacgtatacg attgatgaag caggttttcc tgtgcttgtc agcttgtatg
     1201 tgggaatagg tttccagaat tttgtcaatg cacgaacaga aagcttggct gttttgatat
     1261 ttggtctatt tattgtttgg gcaactgata tcggagcata tatggttggt cgacgttatg
     1321 gtcagcataa gttaagtcct gatatttcgc ccaacaaaac gattgaaggt gcgctcggtg
     1381 gtattggtag tgctgttgta gtcgcatttc tttatttatt gctataccca gcaaaagaac
     1441 tatttaatca tggtatgttt gtcatgcttc tgatgacgat tattttttca gcagtcggac
     1501 aatttggtga tttggtcgaa tctgcaatca aacgtcatta tgatgtgaaa gactcgggaa
     1561 atatcttacc aggccatggt ggaatcttag accgtttcga cagtttgctg tttgtatttc
     1621 ctatcatgca tttatttggc attatttagt aagtatacct gcgtttcttt gtgaatttca
     1681 taaaagacgc aggtttttat ttttcattat attgatacta ttggtgtctt tggtatacta
     1741 tggtaaaatt atagtgtttt atcgttgttt tataaaaaac acaaaaaaca agtaaaggag
     1801 aaaaaagcta tgaaaacgat tctgacattt atcatcgttt ttggtatatt agtgattgtt
     1861 catgagtttg gtcatttctt ctttgcgaaa cgatcaggaa tcctcgttcg cgaatttgct
     1921 atcggtatgg gtccgaaaat ttatgggcat caagcaaaag acgggacaac atatacgctt
     1981 cgcctattac ctattggcgg ttatgttcga atggcaggca atggggatga tgaaacagaa
     2041 atggcaccag gaatgcctct ttctctttta ctaaattctg acggcatcgt agagaagata
     2101 aatttaagta agaaaatcca attgacaaat gctatcccga tggaattgag ccgttatgat
     2161 ttagaagatg agctgactat cactggttat gtgaatggag acgaaacaga agtagtaacc
     2221 taccctgttg atcacgatgc gacgattatt gaaaacgacg gaacagaaat cagaattgcg
     2281 ccaaaagacg tacagttcca atcggctaaa ttgtggcagc gtatgctgac caactttgct
     2341 ggacccatga ataactttat tttggcaatc ctgttattca tcattctggc atttatgcaa
     2401 ggtggtgttc aagtgacgaa cactaatcgc gtaggagaga tcatgcctaa tggagctgcg
     2461 gcagaagcag gcttaaaaga aaatgatgag gtagtcagtg tggacggaaa agaaatccat
     2521 tcatggaatg atctgacaac agtcattacg aaaaatccgg ataaaacatt agactttaag
     2581 atcgaacgag aaggtcaggt gcaatctgtc gacgtgacac cgaaatctgt agagtcaaat
     2641 ggcgaaaaag ttggccaact tggtataaaa gccccaatga atacaggttt tatggataag
     2701 atcattggag ggacgcgtca agctttcagc ggttcattgg aaattttcaa agccttagga
     2761 tcactattta caggtttcag tttagataaa ttaggcggac ctgtcatgat gtaccaattg
     2821 tcttcagaag cagcaaatca aggtgtaaca actgttatta gtttgatggc gcttctttca
     2881 atgaatctcg gaatcgtcaa tctgcttccg attcctgcct tagatggcgg gaaattagtc
     2941 ttgaatattt ttgaagggat tcgcgggaaa ccattaagtc aagaaaaaga aggaatattg
     3001 acattagctg gctttggatt tttaatgttg ttgatggtgc tagttacatg gaacgatatt
     3061 caacgattct ttttctagaa aaagttgaca aatagggtat aataacaaac gataactatt
     3121 ttgaggaaat aaaggagtat atagaatgag acaatcaaaa atgttgattc caacattacg
     3181 cgaagttcca aatgatgcag aagtattaag ccatcaaatc ttgctaagag ctggatatat
     3241 ccgccaagta tcagcgggta tttattcata tctgccatta gcaaatcgtg tattggaaaa
     3301 actgaaacgc attatgcggg aagaatttga aaaaatcgga gcggtagaaa tgctgatgcc
     3361 tgccatcttg ccagcagaac tttgggaaga atccgggcgt tacgaaacat atggaccaaa
     3421 cttgtatcgt ttcaaagacc gcaatgatcg aaaaatgatc cttggaccaa ctcacgaaga
     3481 aacttttact gatttgatgc gtaatgaaat caattcatat aagaaattac ctttaaatct
     3541 ttatcaaatc caacctaaat atcgtgacga aaaacgacct cgcttcggtt tgcttcgcgg
     3601 aagagagttt atcatgaaag atgcgtattc tttccatgca acggaagaaa gtttggacga
     3661 gacatataaa gattacgaaa aagcctacac cgaagtattc aagcgttgtg gcttagaatt
     3721 tcgctcgatt atcggagacg gtggcgcaat gggcgggaaa gactcaaaag aattcatggc
     3781 gatctcagaa atcggggagg atacgatctg ttattcgact gaaagtgatt atgctgcaaa
     3841 cttggagatg gctacaagct actatgtatc aaaaaaatcg catgaaacac agcttgaatt
     3901 ggagaaagta gctacaccta acgtaaaatc aattgacgaa gtagcagatt tcttttctgt
     3961 tgaacctcaa aaaatcatca agtctgtatt gttcattgcg gatgaacaac ctgtccttgt
     4021 tttggtacgc ggagaccatg aagtcaatga cgtaaaactg aaaaactttt tatctgctga
     4081 ctttttagaa gaagcaacag aagaagaagc acaaaaatat ctaggtgctg agtttggttc
     4141 tgtcggacca gtcggtgttt cagatgacgt aaaagtatat gcagaccgtt atgtccaaga
     4201 tttagcgaat gccattactg gagcgaacga aacaggattc cactataaaa atgtgaatcc
     4261 agaacgcgat ttcactgtac tttcttatga agatcttcgt tttgtacaag aaggagatcc
     4321 ttcaccagac aacaatggtg ttcttgcctt tactcgagga atcgaaatcg gacatatctt
     4381 caaaatcggc actcgttaca gtgaatctat gggagcaact gtactagacg aaaatggacg
     4441 agaaaaattt gtgattatgg gctgttatgg tatcggtgta agtcgtttat tatcagcaat
     4501 cgtagaacaa cattcagatg aacaaggaat caactggcca aaaggaattg cgccatttga
     4561 tttgcatatc gtccaaatga atctaaaaga cgaataccaa acaaacctga cgaatgaact
     4621 agaagaagcg atgacaaaag ctggctatga agtactggtt gatgacagaa atgaacgcgc
     4681 aggagtgaaa ttcgcggatt ctgatttgat tggctgtcca atcagaatca ctgtcggtaa
     4741 aaaagcagtg gatggaatcg tagaagtgaa aatcaagaaa actggcgaaa tggttgaagt
     4801 acgtaaagaa gaattggtga atactttacc aatcctcttg aaccaaggcg aataataagc
     4861 gttaaaacac agaaaataaa aatagctgaa tcagctgttt ttgacaatct gatgattcgc
     4921 ttttaaaatt ggaactgcga tttgaatcgc agttccaatt ttttaatgta aaactcagaa
     4981 aaaacgagta tactaaaagg tactgaaaaa gaagaggata agatgcgatc aattgcattg
     5041 tttctcatca gaaagggcgt atatctttgt cagaaaaacg agatttattt gaaaaactgt
     5101 tggaacaaat ccaactagag gaagcagaaa aaaatcatcc actgcttact gctggtgaaa
     5161 tggatagtgt agtggtccat cgtaaatcgc gtttatggga atttacgctg catttttcca
     5221 aaattttgcc tattatgcta tatcggtcat tgactcagca tctgactatc gcttttaaag
     5281 atattgcaca agtcaaactg aatatcttag cagatgacca gacattcgat gaacagctct
     5341 tgcaagatta ctgggcccaa gctttggaaa atcagcaatg cgatacacca ctagcacagc
     5401 aagtattgaa aacgcaggta cctgtgataa aagatcgtaa agtcatcttg ccaatcgaca
     5461 gtacaggagc tatttcttat ttgaagcaac aatacttgcc attagtagaa gagttgttcg
     5521 tcagttacgg attcccaaaa tttcgtatcg aaccagaagt ggatgagcag caagcggaac
     5581 gagtattgaa actttttgaa gaacgcaaac aagaacaggc agaagccttt atgaagcaag
     5641 cagctgaaag tctagttgtc cacgaacaaa agaaaaagga aagaaaagag caatcgccag
     5701 ctcttgaagg acccatccac atccagctag gacgaaatat tccagccgat gaaccaacga
     5761 caccaatgat cagtatcgtt gaagaagaac gaagagtcac acttgagggg tacgtattcg
     5821 acaaagaagt cagagaacta cgttcaaaac gcaagatctt gactttgaaa atcacagatt
     5881 atacttcatc atttatcgtc aaaaaattct cgaacggcga aaaagatgaa caagtatttg
     5941 atgcgatcag tgtcggtagc tggttaaagg tacgaggaag tattcaagaa gatacgtttg
     6001 tccgtgattt ggtcatgaat gcgcaagata tcatagaagt caaacataca cctcgcaaag
     6061 attatgctcc tgaaggggaa aaacgagtag agcttcatgt tcatagcaat atgagtacga
     6121 tggatgcaac aaacagcatc tctgatcttg tggcacaagc agggaaatgg ggacaccgag
     6181 ctatcgccat tacagaccac ggtggtgcgc aagcctttcc ggaagcacat agtgctggaa
     6241 aaaaagcagg tgtgaaaatc ttatacggcg tggaagcgaa tgtcgtagat gatggtgtcc
     6301 caattgctta taatgatgca catgaagcat tgagtgaagc cacatatgtc gtatttgacg
     6361 tggaaacgac aggattgtct gcagtatatg acacgatcat cgagctagcc gcagtcaaaa
     6421 tgtataaagg aaatgtgatc gaaagctttg atgaatttat cgatccagga catcctttgt
     6481 cacgaacaac ggtagatctg actggtatca cggatggaat ggttcgagga tcaaagtcgg
     6541 aagaagaagt attgcggatg ttccttgaat tttcaaaaga tacaattttg gtagcccaca
     6601 acgcggcttt tgatatgggg ttcttgaata ctagctacgc aaggtatggt attcctgaag
     6661 cggccaatcc agtcattgat acgttggaat tagctagata tctgtatcct caattcaaac
     6721 gtttcggatt aggtgtgtta tctaaaaaat ttggtgtcag cttggaacag catcaccgag
     6781 caatctatga tgctgaagca actggtcact tagcatggat ctttgtgaaa gaggcaatgg
     6841 acaatcataa catgctttat catgatcagc taaatgagca tatcggagaa ggagattctt
     6901 acaaaagggc tcgacctttc catgtaacga ttttagcaaa aaatcaggca ggactgaaag
     6961 atttgttcaa actgatttca atgtcgaatg tcgaatactt cgaacgcgtc ccacgaatac
     7021 cacgctccca attgaaaaag atgcgtgaaa atctattgat cggctcagct tgtgataagg
     7081 gagaaatatt cgaagccatg atgcaaaaag gggtagaaga ggcaagaaac cgagcaaaat
     7141 tctacgatta tattgaagtt atgccaaaag cggtctatgc gcctttgatc gagcaagaat
     7201 tggtaaaaaa tgaacatgat ttggaagaaa ttatccaaaa tttggtagaa atcggaaaaa
     7261 gtttggataa aatcgttgtt gcaacaggta atgtacatta tctgaacgaa gaagatgcca
     7321 tttaccgaaa aattttgatc aattctatgg gcggagcgaa tccgttgaat cgtcatagtt
     7381 tgccggatgt ccatttccga acaaccgatg agatgctaac tgcatttcac tttttagggg
     7441 aagaaacagc gaaagagatc gtagtagaaa atacgaataa aatcgcagat atctgcgaag
     7501 aagtgattcc agtcaaagat gaactttata caccgaagat tccaggttca gaagatgaaa
     7561 tatcagaatt gagttacaca aaagcaaaac aaatgtacgg agatccgtta cctgagatca
     7621 ttcaaaaacg attaaaaaaa gagttgaatt ctattaatgg taatggcttc tcggttattt
     7681 atctgatcgc ccaaaaacta gtgcataaaa gtaatgaaga tggctatcta gtaggttcac
     7741 gtggctctgt tggctctagc tttgttgcga cgatgacagg aatcactgaa gttaatccgt
     7801 tagcccctca ttattattgt ccagagtgcc aatattccga gttttttgaa gatggtacgt
     7861 atggttctgg atttgacatg ccagaaaaac aatgtccaaa atgtggtgca cgcttaaaca
     7921 aagacggtca tgatattcca tttgagacat ttttaggatt ccatggagac aaagtgcccg
     7981 atattgattt gaacttctca ggtgattatc aagcagaagc ccataactat acgaaagtat
     8041 tgtttggcga agactatgtt taccgtgccg gaacgattgg tacagtcgcc gacaaaacag
     8101 catatggcta tgtaaaagga tacgaaaggg acaataatct gcagtttcga agtgcagaag
     8161 tcgacagatt agcaaaaggc gctactggag tcaaacggac aaccggccag catccagggg
     8221 ggatcatcgt tattcccgat tatatggatg tctatgattt tacgccgatc caatatccag
     8281 cagacgatca aaattcggaa tggaaaacga ctcattttga ttttcactcg attcacgata
     8341 atgtattgaa attagatatt ctagggcatg atgacccaac tgttattcgg atgcttcaag
     8401 atttatcagg gatcgatcca caaacgatcc ctacggatga cccggaagtg atgcggatat
     8461 ttgccggtcc tgaagtactt ggagtaagtc aggaacaaat ttattccaaa acagggacat
     8521 taggtattcc agaattcggc actcgttttg ttcgagggat gctagaagaa acgcatccta
     8581 caacttttgc agaactactg caaatttccg gactatctca tggtacagac gtatggttag
     8641 gaaatgcgga agaattgatt cgacgaggag atgcgacact ggcggaagta atcggctgtc
     8701 gtgatgatat tatggtttat ttgatccatg ctggattaga tagcgggatg gcatttaaaa
     8761 tcatggaaac tgttcgtaaa ggacaatgga acaagatccc tgatgaattg cgagaaacct
     8821 atctatcggc gatgaaagaa aataatgtgc ccgactggta tatcgattct tgttcaaaaa
     8881 tcaaatatat gtttccgaaa gcccatgctg cagcttacgt attgatggct cttcgtgtcg
     8941 cttatttcaa ggtttatttc cctattttgt attactgtgc atacttttct gtacgtgcag
     9001 acgactttga tctagtctcc atgtgcaaag gaaaagatgc tgtgaaacaa gcaatgaaag
     9061 aaatcacgga caaaggatta gatgcttccg taaaagaaaa gaatcaactg acagtgctgg
     9121 agttagccaa tgaaatgctg gaacgaggat tcaaatttgg aatgattgat ttgtataaat
     9181 cggatgccgt taactttgtt attgaaggtg atacgttgat cgcaccattt agagctgtcc
     9241 caagtttggg aaccaacgtg gcaaaacaaa tcgttgaagc aagaaaagac ggtccatttt
     9301 tatccaaaga agatcttgcc acccgcggta aagtttcaaa aacattgatc gaatatatga
     9361 acgacaatgg cgtattgaaa gatctaccag atgaaaatca attatcgtta ttcgatatgc
     9421 tataagcatc tgttttcctg tctgcttgta attttaagca gagtatggta tagttcttat
     9481 aggtaatgat actaactgtg agtgagcgga tttttcgctc actcttttta atggaattac
     9541 gcacttaatt ttttaaggag gcgaaacttt tgagtagcgt cgttgaaaca gtcacagaaa
     9601 tggtgacacc gattttagat gaacaaaatt ttgaacttgt cgaagtcgaa tttgtaaaag
     9661 aaggaaaaag ctggttctta cgagtattta ttgacaaaga aggcggtatc gatatcgaag
     9721 aatgcgcctt tgtcagtgag aaactgagtg aaaaacttga tgcagcagat ccagatccga
     9781 ttcctcaagc ttatttcctg gaggtatcct ccccgggagc agagcggcca ttgaaaaaag
     9841 aaagcgacta tgaaaaggca cttggtgatt atgtccatgt ctccttatac caaccagtag
     9901 atggcgaaaa acaatatgaa ggatttcttc aatcatttga tgctgaacag cttacattga
     9961 aaatacgcat aaaaacacga gaaaaagaat tgacttttga ccgaaaaaat attgcaaaag
    10021 ctcgcttagc tatccaattt taatcacgga gaggaatcag aaaaaatgag taaagaaatg
    10081 ttaaatgcgt tagacgcttt agaagctgaa aaagggatct caaaagaaat cgtgatcgat
    10141 gctttagaag cagcattggt ttctgcatac aaacgtcact atggccaagc acaaaacgtc
    10201 gaagtggaat ttgatcagaa aaaaggaaag atccatgtct atgcggtaaa agaagtaacg
    10261 gaagaagtga tggattcaca gttggaagtc tcattgaaag atgcactttt aatcaatcca
    10321 gcatatgaga tcggtgatac gatccgtttt gaagtaacac caaaagattt tgggcggatc
    10381 gctgcgcaaa cggctaaaca agttatttta cagcgtgtac gtgaagcaga aagaacgatt
    10441 atttataacg aattcagtgc ttatgaaaaa gacatcatgc aaggaatcgt cgaacgccag
    10501 gataaacgtt atatttatgt caatcttggt aaaattgagg cagttctttc taaacaagat
    10561 caaatgccaa atgaatttta tcaaccacat gatcgaatca aagtttatgt ttcccgagta
    10621 gaaaatacgt ctaaaggtcc gcaagtattt gttagccgca gccatccaga cctattgcgc
    10681 cgcttatttg agcaagaagt accagaagtc tatgatggaa tcgttgaaat tgtcagcatc
    10741 gcccgtgaag ctggcgatcg ttcaaaagtg gcagttcgct caaatgatcc aaatatcgat
    10801 gcagtcggta catgtgtcgg gccaaaggga caacgagttc aagcaatcgt caatgaatta
    10861 aaaggcgaaa acatggatat cgtcgaatgg aatgaagatc cagctgtatt cattgcaaat
    10921 gcattgaatc ctgcacaagt catggatgtg atcttcgatg agacaaatcc aaaggcctgc
    10981 acagttgtcg ttccagacta ccaattatcg ctagcaatcg gtaaacgcgg acaaaacgca
    11041 cgtttagctg ctaaattgac taaccataaa atcgatatca aatcagaatc tgacatgacg
    11101 gaattttatg aaaatcaaga acaacaaaaa gaaacagaag aactgcatga tgaagcaatc
    11161 gttcagtcag atttaacaga cgatgaatat gaaacaattg cattcaacaa tgaaacggtc
    11221 gaagaaaaac cagaagctta actttagtaa ggaggcaaag agatgaaaca aaggaaaatt
    11281 cctttacgca aatccgtggt ttcaggtgaa atgaaaccta aaaaagagat ggtccgaatc
    11341 acccgttcaa aagaagggga agtatcgatc gatccaactg gaaaaatgcc tggacgagga
    11401 gcgtatgtct cacttgagcc ggaagaagta caacaagctt gggataaaaa aattctcgat
    11461 cgagtactcg aaacaacatt aacagatgag ttttatcaag aacttcttga ctatgtcact
    11521 caccaaaaag cacgtaaaga gttatttgga aaatgaatac agttaatcgg caaaaagcca
    11581 tgaatctcat cggtctagcg atgcgcgcgg gaaaactaat cacgggtgaa gaactgacca
    11641 ttgctgaaat ccgcaaacaa aaagcaaaat tggtgtttgt ggcaaccgat gcaagcgaga
    11701 atacaagaaa aaagataaaa gataagagtt catattatga agttccttgc tttgagctat
    11761 tttctgaagc agaaatcacg cagatgatcg gaaaacctcg caaggtattc ggaataatgg
    11821 atgccggatt tgcgaagaag accaaggagc taattgaagg ttaggaaggt gattgcatgg
    11881 gcaacaaaag aatctatgaa ttagcaaaag aactcaaaaa atctagtaaa gacgtcgtgg
    11941 aaaaagcaca acaacttggc atcgatgtga aaaaccatat gggtgcgatt actagcaatg
    12001 atgagaagaa attgcaacaa gcgttcaaaa acccaagcac gaaacaacaa gctgcacaac
    12061 aagcaagcag aaaaccagcc aatcaacagc cgaacgatca acgtaaaaaa gaaacaacga
    12121 acaatcaaca aaaaaataaa aataaccgca gttatcaaga tcgcggtcaa gggagcggtc
    12181 aagtgaatca aggaaaaaaa ccaacaacaa accaaaataa tacgggaaat accagcagcc
    12241 aaaatcgtca aggaacgagc caaggcggca atcaaaaccg tactaacaat aacaacaaac
    12301 caaacaataa caatcgcggc aaatttaata ataataaccg taatcgttat aataaaaaag
    12361 gtaaaaaagg caaacagcaa caatcaacta aaccagctgt accaccacgc aaattccgtg
    12421 aattgccaga tgtattagag tatactgaag gtatgaacgt agcagatatt gcgaaaaaaa
    12481 ttcatcgtga gccagcagaa atcattaaaa aattatttat gcttggtgtc atggtcaatc
    12541 aaaaccaagc actggataaa gatacgattg aactgttagc gacggactat ggcatggaac
    12601 cacaagaaaa agtccaagtt gacattgctg atatcgataa attcttcgag gcagaagaag
    12661 tcgatccaga taaattagtc tcacgtccgc cagtcgtaac gattatggga cacgtagacc
    12721 atgggaaaac aactttattg gatacattac gccattcacg tgtaacaagc ggcgaagccg
    12781 gcggaatcac acagcatatc ggtgcctacc agatcgatat tgatggtaaa ccaatcactt
    12841 tcttggatac accaggacat gcggccttta caagtatgcg tgcacgtgga gcaagtatca
    12901 cagatatcac gatcttagtt gttgctgcag atgatggcgt catgccacaa acagttgaag
    12961 caatcaacca tgccaaagct gcaggcgttc caatcatcgt cgctgtaaat aaaattgata
    13021 aaccaggtgc aaaccctcaa catgttatgc aagaattaag tgagtatgag ttaattcctg
    13081 aagcatgggg tggagaaacg atctttgttg agatctcagc gaaattcggc caaaacatcg
    13141 aagaattgct ggaaatgatc ctgttagtcg cagaagtaga agatttaaaa gcagatccaa
    13201 cacaacgcgc aatcggaaca gtgatcgaag cacgtcttga taaaggtaaa ggtcctgtgt
    13261 caaccttact tgtccaagaa ggaacacttc atgttggtga cccaatcgtt gtcggtaata
    13321 cttacggacg tgtccgtgta atgacaaacg atcttggccg tcgcgataaa gaagcaggac
    13381 ctgcaacgcc agtagaaatc actggcttga atgatgtacc tcaagcaggt gaccgtttcg
    13441 tagtcttcga agacgaaaag acagcacgtg cagcgggtga agaacgcgcg aaacgtgcat
    13501 tattagaaaa ccgtgctgcc acaagccgtg tgactttgga taacttattt gaaagcctga
    13561 aagaaggcga attgaaagaa gttaacgtaa tcatcaaagc agatgtacaa ggttcagcag
    13621 aagcactggc tgcatcattg aagaagattg atgtggaagg tgtccgtgta aaaatcgtcc
    13681 attcagctgt cggagcaatc aatgaaagtg atgtaacctt agcagcagcc agcaacgcta
    13741 ttatcatcgg tttcaacgtt cgtcctacac cgcaggcaaa agtgcaagct gatactgaag
    13801 aagtcgatat tcgtcttcac cgtatcatct ataaagccat cgaagaaatc gaaactgcga
    13861 tgaaagggat gctagaccct gaatttgaag aaaaaatcac cggtcaaatg atcgttcgtg
    13921 aaaccttcaa agtttctaaa gttggtacga ttgcaggtgc ctatgtaaca gaaggatata
    13981 ttcgtagaga tagcggtgtc cgtgtaatcc gtgacggaat cgtaatctat gaaggccaac
    14041 ttgcaagtct gaaacgattc aaagatgacg tgaaagaagt caaaatgggt tatgaatgtg
    14101 gtgcaatgat tgaaaaattc aatgacatca aagtagatga cgtaattgaa ggatttatca
    14161 tggaagaagt caaaaatgac taaaaactga taaaaggagg ccaatcatta tggctaacta
    14221 ccgtgaccgc cgagtcggtc aagaaatctt aaaagaagtc aatgatattt tgcgcaaaaa
    14281 ggtccgtgac cctcgtgtgg agaacgtaac gatcacagat gtgaaagtta cgggggactt
    14341 acagcaagca acgatctatt acagcatttt atctgactta gcatcagata agaaaacagc
    14401 acaagatgga ttgaataaag catcaggatt gattcgtcga gagttagggc acaatctaag
    14461 tatttataag acacctgaat tgttctttga acttgatgaa tcagttgtct atggcaacca
    14521 tatcgatgaa ttgcttcgcg acttgaacaa agactaatca aaaactcccg caaatcctgt
    14581 gaaatcaggg gttgcgggat ttttttgttt tcattaagac gtattaccta agagttccat
    14641 gactttagtc ttgcggcgtg tcagctaacc aaaaaagaga aaaagaaaag taaaaaattc
    14701 gactttcttt tttctagtag tatataaatt tttgcatata gataaagatc aagataccga
    14761 taacggaagc tagattgtaa tctattttag tgacagtcag gtatagccaa gtaactagaa
    14821 gatcagtgga acctgcacca taaaagtttg agtttgtcgg tccgttgaca gttaaaagaa
    14881 aaatcacgtt gaagttattg atatttccga ttacgccgct tcgatttggt cacgatccaa
    14941 attttggata atccctgtag aaattagcat agtagctggg ataccaattc acatattgac
    15001 aaagataaca gagactttta cataaaacgg atcaatcagg aaaggaattg ctcgatccag
    15061 ccccagtttt ccagcaatgt gttaactggc ccagcgtcat tcagcaaatt atgcatgatg
    15121 aggagcgaaa tgaactgcgg aacagccatt gtgataacga caagtttttt ggatcaatgt
    15181 aaaaaacgtc agcgaaagga aaaatttcgt ggttttttat acctgttcat ttggctagat
    15241 ataaaatcca aacaaatgaa cagaagtgct tattaggttt caattgggag tatctgcgct
    15301 agaataagag taataatgaa aaggagtgtt atgtatggat tattctagta aagaatactt
    15361 tgataaaatg acggcttggt ggcgtgctgc taattatctt tctgttgggc aactttatct
    15421 taaagataac cccttactga gacgtacact gaaacccgaa gatgtcaaaa aacatccaat
    15481 cggtcattgg ggaacgattc ctggccaaaa ttttatttat gtccatttga accgagtcat
    15541 caataaatat gatttgaaca tgttttatat tgaagggcca ggacacggcg gacaagtcat
    15601 ggtatcgaat gcttatttag atggaagtta cacagagatc tatccagaag taacggaaga
    15661 tgagacagga atgcaaaaat tattcaaacg attttcattt cctggaggca ttgcttctca
    15721 tgcagcacca gaaacaccag gttctatcca tgaaggcgga gagcttggat actctctttc
    15781 acatgcagtc ggagctgtat tagacaatcc tgaagtaatc tctgctgtcg tgattggtga
    15841 tggtgaagca gaaaccggtc ctttagctgg aagctggttc tcaaatgtct ttatcaatcc
    15901 agtgatagat ggagctgttt taccgatcct tcatttaaat ggtgcaaaaa tagccaaccc
    15961 aacgatcttg gcacgtaaat cagatggaga attagcaaat tactttaacg gacttggctg
    16021 ggaacctttc tttattgaag gaaatgattc agaaaaatta aatcctgtta tggctgaaaa
    16081 aatggatcaa gcaatcgaga aaattaaatc catccaaaaa gaagctcgcc tgaagacagc
    16141 aacagatgtc gtgatgccaa aatggccagt attgatcgta cgcacaccaa aaggatggac
    16201 cggtccaaaa gaatgggacg gggaaccaat tgaaggaacc ttccgagcac accaagtacc
    16261 gattcctgta gatcaagaac atatggatca tgcagatgct ttactccgtt ggctgaaatc
    16321 ctatgaacca gaaaaattat ttgatgcaca aggacgtatt ttagaagaaa tcagagaaat
    16381 cgcgcctact ggtgaccaaa gaatggcaaa aaatcctatc accaatggtg gaatcgatcc
    16441 aagaccgctt attatgccag attggaaaaa atatactttg caatttgaaa aaccaggctc
    16501 cgttaaagca gaagatatga ccgaacttgg taaattcgtt cgcgagatca ttgagaaaaa
    16561 tccagaaaat ttccgtatat ttggaccaga tgaaacaaaa tcaaaccgat tgaatcaagt
    16621 tttcaaaacg acaaatcgcc aatggatgga aaaaatcgaa ccagaaaatg atgaatggct
    16681 ttcgccatct ggtcgcgtca tcgacagtca attatcggaa caccaagatg aagggttctt
    16741 agaagggtac gttctgactg gacggcatgg ttttttgcaa gttacgaatc attcttgcga
    16801 gtggttgact ctatgttgac tcagcacttc aaatggatgc ggaaatcaca tgacctttct
    16861 tggcgaaatg attatccttc gctaaatctc attgcaagct caacagtctt ccagcaagat
    16921 cacaatggat atagccacca agatccagga atattgacac atttggcaga aaagaaggcg
    16981 gagttcatcc gtgaatatct gcctgcggat gcaaatacat tgctcgcagt catggataaa
    17041 gcattccgct cgtcagaaaa aatcaatttg atcatctctt ccaaacatcc gcgtgctcaa
    17101 ttttactcag cagaagaagc agccgtctta gtgaatgaag gcttgaaaat cattgattgg
    17161 gcttcgacag caaaagagga ggaacctgaa ttggtcattg ctgctgcagg aacggaatct
    17221 aacttagaag cattagcagc tgtaactttg ctgctggaag aattcccaaa actaaaaatt
    17281 cggttcatca atgtcgtaga tttattgaaa ttacgtcatc caagtcaaga tccgcgtggg
    17341 ttgagcgatg aagaatttga tcaatatttt acaaaagaca aaccgatctt atttgctttc
    17401 cacggttatg aaacattggt ccgcacgatt ttctttgatc gtcataacca tcatttaatg
    17461 attcatggct acaaagaaaa tggagatatc acgacaccat ttgacatgcg tgtagtaaat
    17521 gaacttgatc gttatcattt ggcaaaagac gcagctttaa aaatcaaagg aagtcaagca
    17581 gaagattttg cgaaaaaaat ggatcagaaa ttacaagaac atcaaaacta tattcgtgaa
    17641 aatggtattg atctgccaga agtattggat tggaaatgga aaaatcttga ccaataaaat
    17701 gataaaatcg atcccgcaaa atagaatttt ttacattgat taagaaactg aaaaaataaa
    17761 taaaaaaaac ggaaaaaaga ctggaagaat ggccagtctt ttttttcgtt tttgggaagt
    17821 gaaaaccaaa ctatttacct gtaagccacc cataaatcca atcaaatggt gcaaagaaaa
    17881 aatcttccat tctattccct cctttaaaaa agcataacat gatttctatt tacgtgagca
    17941 tgaattttgt tacgttttta ttgccgaaaa ataaaaaccg ttcattatga tgtcaaattc
    18001 gtcagtaatt gatgcaatgt gttataataa ttcagttaaa tcaaaaatga aatggagaaa
    18061 agacatggac gggttattac ctttatggaa agaacgcgga atgacaagtc atgattgcgt
    18121 attcaaatta agaaaaatct tacatacgaa aaaaatcgga catgggggta cgcttgaccc
    18181 agacgtagac ggtgtgttgc ccatttgtat tggaaaagcc acaaaagtta tcgaatattt
    18241 gacagattct gggaaaatct atgtaggcga gataactcta ggtttcagta caacgacgga
    18301 agatcgatcc ggagaacggg tagaaacaaa accagtagat atgtctttat ctgaaaaaaa
    18361 aatagatgaa gtgatggctt catttattgg tgagatcacc cagataccac caatgtactc
    18421 agcggtaaaa gtcaacggta agcggttata tgaatatgcc cgcaataatg aaacagtgga
    18481 gcgtcctgtt agaaaggctc agatttaccg ttttatccgg acaagtgaat tgacaagaga
    18541 cgaagtgaca ggaaccattt cttggcgctt cgaagtagaa tgcgggaaag gaacctatgt
    18601 ccgcacatta gctgtagata caggcaagaa acttggttac cctgcgcaca tgtctgatct
    18661 gacccgaaca gcaagtgccg gtatgtccca gaaggatgct ctgacattag aagaagtagc
    18721 tcaagctgtg aaaaatggga caatcgatga ttgtttgctg ccaatagaaa aaggagtgga
    18781 acggttcaaa cgagtagata ttactgatga agtatggcag aaagtaaaaa acggtatgag
    18841 acttgattat actgtatttg cgctcccaga aatgccgtca gaagaaattg ctttgtttta
    18901 taaaggaaaa gttgtcagta tttatcagcc aaacccgaaa gaaaaaaaca aattaaaacc
    18961 tagtaaagta ttgagaaatg aggaataacg aatgaatatc atcaagatca gacatcctta
    19021 ccaatcagaa caaattccag aagacgatgt cgttttagtg ctgggatttt tcgacggcgt
    19081 gcatatcgga caccagaaag tcataaagac aggtatggaa attgcacaaa aagaaggatt
    19141 gaaattagca ttgatgactt tcaatcagca tccatctatt gtctttaaaa agatcgatcc
    19201 tagcagcgtg aaatatttga ctagccttaa gcaaaaagaa gaaaaaatgg ccgctttggg
    19261 tatagattat ttatatgaag ttgattttac ttcttctttt gctcatttag caccgcagga
    19321 atttgttgat caatacatcg tagggttaca tgcaaaatat gctgtttccg gttttgatta
    19381 tacgtatgga ccaaaagaca ttgcagatgt cgctcatttt cctgaatatg ccaaaggaag
    19441 attcaaaatc gtcacagttt ctaaagaaga agaacaaggt gaaaaaatca gctctacccg
    19501 tattcgtgga ttactagaca gcggacaagt aaaagaagca gcaaaactat taggttgcac
    19561 atatgaggta gaaggggtag ttgtccatgg cgatgcacga ggcagactac taggattccc
    19621 tacagcaaat gtcaaagtaa agagcacggt tcatttgcct aaagaaggtg tttatgtgtc
    19681 ggaaataaaa attggggaaa catggtatcc agccatgggt tcaattggac ataatgatac
    19741 attcggtgaa ggacgggagc tgactgtaga gttgtatatt ttggacttta atcaagatat
    19801 ctatggtgaa catgtcgcgg tacgctggaa tcaattttta agagatcaag tgaaattttc
    19861 tagtgtagag gaattgattg aacaactcaa ggaggatgaa caagcaacag ctgaatattt
    19921 ttctcagcaa taaaacatta tgcataaaat ctcttgttta tgtataacta gcaattacaa
    19981 taaaatgggt gataaataag cccagga
//
LOCUS       DACCYH010000052        19977 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.053, whole genome shotgun sequence.
ACCESSION   DACCYH010000052 DACCYH010000000
VERSION     DACCYH010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.053"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            299..1960
                     /locus_tag="IVY85_001772"
     CDS             299..1960
                     /locus_tag="IVY85_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8044788.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2392)
                     /locus_tag="IVY85_001773"
     CDS             complement(2030..2392)
                     /locus_tag="IVY85_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP8044789.1"
                     /translation="MKEQSNEKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFL
                     WSLPFLIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLII
                     AGILIALSAIFMLFSLLG"
     gene            2460..3401
                     /locus_tag="IVY85_001774"
     CDS             2460..3401
                     /locus_tag="IVY85_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8044790.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3402..4445
                     /locus_tag="IVY85_001775"
     CDS             3402..4445
                     /locus_tag="IVY85_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8044791.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4451..5521
                     /locus_tag="IVY85_001776"
     CDS             4451..5521
                     /locus_tag="IVY85_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8044792.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5511..6464
                     /locus_tag="IVY85_001777"
     CDS             5511..6464
                     /locus_tag="IVY85_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8044793.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6476..7486
                     /locus_tag="IVY85_001778"
     CDS             6476..7486
                     /locus_tag="IVY85_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8044794.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7771..8592)
                     /locus_tag="IVY85_001779"
     CDS             complement(7771..8592)
                     /locus_tag="IVY85_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8044795.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8689..8967)
                     /locus_tag="IVY85_001780"
     CDS             complement(8689..8967)
                     /locus_tag="IVY85_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044796.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9235..11244
                     /gene="metG"
                     /locus_tag="IVY85_001781"
     CDS             9235..11244
                     /gene="metG"
                     /locus_tag="IVY85_001781"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP8044797.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11723..12310)
                     /locus_tag="IVY85_001782"
     CDS             complement(11723..12310)
                     /locus_tag="IVY85_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044798.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12485..13807
                     /locus_tag="IVY85_001783"
     CDS             12485..13807
                     /locus_tag="IVY85_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8044799.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13962..14738
                     /locus_tag="IVY85_001784"
     CDS             13962..14738
                     /locus_tag="IVY85_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8044800.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14731..15300
                     /gene="rnmV"
                     /locus_tag="IVY85_001785"
     CDS             14731..15300
                     /gene="rnmV"
                     /locus_tag="IVY85_001785"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP8044801.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15293..16177
                     /gene="rsmA"
                     /locus_tag="IVY85_001786"
     CDS             15293..16177
                     /gene="rsmA"
                     /locus_tag="IVY85_001786"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP8044802.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16628..17257
                     /locus_tag="IVY85_001787"
     CDS             16628..17257
                     /locus_tag="IVY85_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044803.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17381..18490)
                     /gene="ugpC"
                     /locus_tag="IVY85_001788"
     CDS             complement(17381..18490)
                     /gene="ugpC"
                     /locus_tag="IVY85_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP8044804.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18699..19121
                     /gene="mgsA"
                     /locus_tag="IVY85_001789"
     CDS             18699..19121
                     /gene="mgsA"
                     /locus_tag="IVY85_001789"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP8044805.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19444..>19977
                     /locus_tag="IVY85_001790"
     CDS             19444..>19977
                     /locus_tag="IVY85_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8044806.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT         6501 a         3589 c         4077 g         5810 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgtggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt tttcatttga ttgctccttt atttttggtt
     2401 cattatctaa taatgtagcg aaacatctgt cagaagtcaa aagctagggg gaaacattag
     2461 tgaatagtta tatcaaatat gtattaaaac gtgtcttttt catgatcatc acgctttggt
     2521 taattgcaac aatcaccttt ttcttgatgc aattgctgcc gggcacgcca tatacgaatc
     2581 aggaacgttt gagtcctgaa acaatcgaga tgctaaataa acaagtcggt ttggataaac
     2641 cagttatcgt acaatatggg atttatcttt ctaatttgct tcaaggagat tttggaattt
     2701 ccttccaatt taaaaaccag ccagtcgcac atttgttagc tggaagaatc ggaccatcct
     2761 tgcagttagg gttacaggca attatctttg gaacagtcct agggacgatt ttaggaacga
     2821 tctctgccat gaagcaaaat acatgggcag atacatcttc tacactcgta gcgatattag
     2881 gtcgttccat tccaaacttt gtatttgctg ttttattaca atatatcttt gcaatcaaat
     2941 tacaagtttt gccaatcgca aaatgggatg gctttgtgta cacaatctta ccaacaattg
     3001 cgttggccat gtcgccgctt gctgactctg ctcggtttat ccgtacggaa atggtggaag
     3061 tactgcatag tgattatgtg gaattagcaa aagcaaaagg attgagccgt tgggaaatcg
     3121 cctttaaaca tggtcttcga aatagtttga ttccattgat gactttatta ggaccgttag
     3181 ctgtagcatt gatgaccgga tcactggtgg tagaaaatat ctttgctatt cctggtatcg
     3241 gggaacaatt cgttaagtca atcacaacaa atgattatcc tacgatcatg gctgtaacga
     3301 tcctttactc atttatgctg atttttgtta ttttgatcgt ggatctattg tacggtctag
     3361 ttgatccaag aattcgtgtt tcagaaggga gccgaggata aatggaaatg ataccaaagc
     3421 gttatgaaac agttgcagat atccctgcgg atgaatttct tccattacaa aaaaataccg
     3481 aagaagaacg tgaacagatt actgcacctt ctttgaattt tatccaagat tcatggcgcc
     3541 gtttaaagaa aaacaaagct gccgttattt caatggtttt actattggtc atcattttga
     3601 tctctgtgat cacaatcttt gtttctcctc atgatccaac tgcccaaaac gtggcttata
     3661 tcaatttgcc tcctcgtatt ccgggaatca atatcgatgg attgaatgga aaagcaatgg
     3721 ttggtggtga acttgttgat aaatatgctc aagcaaacgt cccttcaaac gtaaatttct
     3781 ttttaggaac agacggtttg ggacgtgatg tattaagccg cttatttatg ggaatgagaa
     3841 tctcgttgct gatcgcattt attgctgcat tactagatgt aacgattggt gtagcttacg
     3901 ggttggtttc tggtttgtta ggcggacgtg tcgataacgt gatgcaacgt tttttggaaa
     3961 tcttatcagg aattccaaac ctcgttgtca tgattttgat gttggtcgtt tttgaaccag
     4021 gaattttttc aatcgtggct gctatggcca tcacaaactg gataccaatg gcacgtatcg
     4081 ttcgtgcaca aacaatgaaa ttaaaagatc aagaatatgt tttagctgga ttgacattag
     4141 gtgaatcaag aatgaaaatt gcattcaaac atattttgcc gaatatctct agtgtcatta
     4201 ttattcaaat gatgtttagt attccatctg ctattttctt cgaagccttt ttaagcttta
     4261 tcggtttagg tcttacgcct ccgtcagctt cattaggaac gatgttgagc gaagggtaca
     4321 agacattcct gtatctgcct tatctattat ggattccggc agtaacttta tcggttatca
     4381 tgattggctt taacttactt gctgatggat tgcgtgatgc ctttgatcct aaaatgaaag
     4441 agtgaagacg atgacgaaaa ttttagaagt gaaagactta gaaatctctt ttgatacgta
     4501 tgctggaaag gtacgagcga ttcgcggtgt caattttcat ttgaataaag gagaaacatt
     4561 ggcgatcgtt ggggaatctg gaagtggaaa atcagtgacg accagaagca tcatgcgctt
     4621 gctgtcaagc aacgccaata tagatgctgg tcaaatcctc tttaaaggta acgatatcgt
     4681 tggaaaatca gaaagagaga tgcaaaaaat ccgtggtaaa gaaatagcga tgattttcca
     4741 agatccaatg acctctttag atccgacgat gcccattggg aagcaagtcg ctgagtccct
     4801 tagaaagcat aataaagttt caaaaaaaga gggacaaaaa gctgcattag aattgcttaa
     4861 attagttgga atacccgaag ctgagaaacg tatcaacagt tatccgcatc agttctcagg
     4921 aggtcagcgt caacggatcg tgatcgcgat tgcactggta tgttatccgg aaatcctgat
     4981 tgctgatgaa cctacaacag cgcttgatgt gactattcaa gcacagattt tggagctgct
     5041 gaaagaaatt cagacaaaaa tcgatacatc aattatcttt atcactcatg accttggtgt
     5101 agtagcgaat gtcgctgatc gtgtcgctgt catgtatgga ggaagaatcg ttgaagttgg
     5161 aacatcggaa gaaattttct acaacccgca acatccctat acttggggac tgcttggttc
     5221 aatgccgaca ttagatagtg cgaacgatcg attgtatgct attcctggtt ctccaccgga
     5281 tttattgaac ccgccaaaag gagacgcatt ctatccaaga aatgaattcg cgatgaaaat
     5341 cgatgcagaa caagaaccac cattttttga actatcaaaa acacacaagg cagcaacttg
     5401 gctacttgct ccgcaggcac ccaaagtgac accgccagaa gaaatccaac gtcgctgggc
     5461 tatctttaaa gagaaacaaa aacatactta cggtcaagga ggaattgctg atgccaaata
     5521 aactacttga agttaaagga ttgaaacagt atttcaatgt aggaaaaaag aatgaagtac
     5581 atgctgtaaa tgacatcagc tttcacattt atgaaggaga aacattcggt ttagttggag
     5641 aatctggaag cggaaaatcc actactggcc gtacgatcat tcgcttgaat gaacctactg
     5701 atggtgagat attattcgac ggtcaagata taacgaagat caaagataaa aaaggtttga
     5761 cgaaattccg gcatgatgtt cagatgatct ttcaagatcc atacgcttcg ttgaatcctc
     5821 gtatgaaagt cagagatatc attgccgaag gaattgatgt taatggacta gctaaatcat
     5881 ctgaagagcg tgcaaaaaaa gtcgatgatc tattgagaac tgttggattg aatccaagcc
     5941 acggtactcg ttatcctcat gaattttcag gtgggcaacg ccagcgtatt ggtattgcac
     6001 gagcattggc agtaaaaccg cgttttatca tttgtgatga accaatttct gcactagatg
     6061 tgtcgatcca agcccaagtg gtcaatttgt tgcaagatct ccaaaaagaa catcaattga
     6121 cctatctatt tattgcacat gatttgtcga tggttaaaca tattagtgac cgaatcggtg
     6181 taatgcataa cggcttgtta ttagagatgg gaacaagtga tgaaatctat aatcacggtg
     6241 ttcatcctta tacagaaagt ttgttatctg ctattcctct accagatcca gaccatgaga
     6301 gaaaacgcag aagaatcaaa tatcaacctg aaccagatga cggacaagta cgtcaacttc
     6361 gtgaaatcgc accagaacat tttgtctatg caactgagca ggaagtccca tattacgcaa
     6421 aaaaactaaa acgacaaaag gaatctcttc tggttgctaa ctaggaggga catagatgct
     6481 aaaagctttt aaattccgta tgtatccgac agaagaacaa aaacagcaat tgattcgtac
     6541 ttttggatgt gctcgattta cctataacca tttgctgaaa aagcggcaga aaagttggca
     6601 acaaacagga gtagccaatt tttcgctcac acccgctaca ttaaaaaaag aatacccatt
     6661 cttaaaagaa gtagacagtc ttgctttagc aaatgcacag cttaatttgg atcgggcttt
     6721 tcgcaattat ttcaagggac gtgccagctt cccaaagctt aaaacgaaaa aaagtatgtg
     6781 gcaatcttat acgaccaaca accaaacccg tacgatctat ctggaaaatg gctacttaaa
     6841 gttaccaaaa caaaaagaac taatcaagat aaatcagcat cggccagttg aaggatcgat
     6901 tcgctccgct acgatttctg ctcgatataa tgaagaattt tatgtcgcac tactttgtga
     6961 tgtttcgcca gtaaaaaaag aatctttagc taaatggatc gggatcgcct atcatccgaa
     7021 gacattgatc caaacatctc gacctctaga agtaacttta ccaaaatttc accagacaga
     7081 agaaaagctg caacatgcac agcggaaatt gaatgtgaaa gtccgctccg ctcaccatag
     7141 aaagatcagg ttagatcagg caagcaatta tcagaaacaa aagagaaaag taatggatct
     7201 ttatctgaaa caaaagaacc agcgagaaga ttatcttgaa caattatctg ggaaattggt
     7261 caaacaatat gactacttat ttgtagaatc ttttccaaaa gaagaggcac atgctgattt
     7321 ttctattcac gactggcata aattaataac aaaattgcgt tataagtccc aatggtataa
     7381 taaaaaattt cttttgatca ataccgacgg agcagaagaa agcaattccg taagaaagag
     7441 ccaagtagta gaaaaaatgg gcagacattc ggttatcaag ggataaaaaa tgaatgaaac
     7501 tgataggagc gttagggtta gccttgttaa taaaaagtct taatgacttt tgtttctagg
     7561 aatttttctt ttgaagaaaa gctcactaat tatgacaaac caataaaaca atagcagaaa
     7621 aagttcgtac gagctttttc tgctattgtt aattttttat tcagaaagtt tacgaataca
     7681 tcaaaattct gaaacaaaaa acgtaactca caaagagttg tttttgcgtt ttttcttgaa
     7741 tgattaggtc tgaatctttt gctcacaacc ttattcatgg tgccccggct tgagctgata
     7801 gatttttgaa ggacggccga ttcctacagg acgttcaccg atctcatcga aatattggag
     7861 catagctttc ttaaaattgg aatgatcgat cgttttataa tcgatcccta agaatttagc
     7921 aaatactttt cgagcttccg taatcgtgaa atctttacct aaaacttgta gaacttgcgg
     7981 ctcgtgttcc attttattga ctacacggtt gaatgctttc aagatgattt gactatggtc
     8041 aaaagccaaa gtatctttgc cagaagactc tccagtgact aaatcaagcg agatagccac
     8101 ttctccgcta gataaatgga tcttgttact atgacgttct agtgtgaacc agcgtacttc
     8161 cttggcatca tctccggcga ttagcggttc ttctccaata aaggctaagt agctgactgt
     8221 gacgacccag cctcgcggat ctcgattagg cgtactgaat gtatgcagtt gctcaatatt
     8281 ttcttttgaa ataaccacac cagtttcttc ctttgtttct cttaagacgc tttctccagt
     8341 tgactcatcc ttttggacaa agccaccagg caatgcccat gaatttcgat aaggatgccc
     8401 ttttctttgg atcaacaaaa ctttcagctg atcttcttct ttgttatagc acatcagtac
     8461 gatatcgaca gtcaaagagg gcttgtcata ttctggacgt tcctgttttt gataccagtt
     8521 aagaaattct tcttcacttg ctttatgttc ataatataat ttttcttctt ctttagaagc
     8581 aaaacggttc atctgaccac ctccattgac agtaaaagta ctttttacct tattgtagca
     8641 gactctgaaa aaaaaccaag gatttacttg aaaaatctag gttatcattt aaatatgaaa
     8701 aataaatgga agtatgagat tgaataaaaa gaaaagcaga taaaacggca atactttata
     8761 ccgctcttca cgaatggcaa agaagccaca aataacgata tatacgtcaa tgataaaacg
     8821 caaaatccaa gctgctgtca taaaactgtt caaatgccca tggaggttaa aataaccata
     8881 catacgaata taaaaatagc tgaatattaa atgtgctaat gaaagaaaag caatactaag
     8941 tgtaaaaaaa agatgtttct tctgcatgat tgttccagtc ccttactaag atagatagtc
     9001 tttattatac cacgtgtgag gggagaaata aaaagccgtt ttttagtaga tatgaaacaa
     9061 aacaaaaacc cgcatttatt caacctattc atccttttaa aggatagatt gaacgtagat
     9121 agaagtcgtt tatccagtct ttttcatttt gaaaaaaagc taacagcatg ctttttttat
     9181 ggtatactag caaggaaacg aattcgcatc atgcgataaa ggaggaagag ccccgtggca
     9241 gaaaaggaaa ctttttatat cactacgcca atctattatc caagcggcaa acttcatatc
     9301 ggcaactcgt atactacgat tgcatgtgac gcaattgctc gttataaacg gttgatgggc
     9361 tttgatgtgt tttatctcac tggagtggat gaacatggtc aaaaaatcga aacaaaagca
     9421 gaagaactag gtgtaaaacc gcaagaatat gtagataaaa tggcggctga tgtcaagaaa
     9481 ttatggaaaa cgcttgatat cagttatgat aaattcattc gcacgacaga tgattaccat
     9541 aaagcagctg ttcaaaaaat atttgatcgt ttattagaac agggagatat ttacttaggt
     9601 gaatatgaag gctggtactc tgtttctgac gaagaattct ttacagaaac acaacttgca
     9661 gaagtgtaca aagatgacga aggtaacgtg atcggtggta aagcgccaag tggacacgaa
     9721 gtagaattgg taaaagaaga atcatacttc ttccgcatga gcaaatacgc agaccgtcta
     9781 ttggaatact atgaagaaca tcccgaattc atccagccag aatctcgtaa aaacgaaatg
     9841 atcaacaact ttatcaaacc aggattagag gatcttgcag tttcacgaac aacttttact
     9901 tggggagtac cagtcaagag tgatccgaaa catgtggtgt atgtatggat cgatgcatta
     9961 tccaattata ttactgcact aggatatggc tccgaagatg aaagtctatt cgaaaaatac
    10021 tggcctgcag atgttcagat ggtcggtaaa gaaatcgttc gctttcatac gatctattgg
    10081 ccaatcatgc tgatggccct tgatttgcct ctacctaaaa aagtatttgg gcatggctgg
    10141 ctattgatga aagacggaaa aatgtctaaa tcaaaaggaa atgtcgttta tcctgaaatg
    10201 cttgtggaac gttatggttt agatgcactc cgttattatt tgcttcgtgc tgttccgttt
    10261 gggtcagatg gtgtctttac gccagaagat tttgtttccc gtgtcaatta tgatctggca
    10321 aatgatctag ggaacttatt gaatcgaacg attgctatga tcaacaaata ttgtgatgga
    10381 ctcgttcctg actatgcctc acttgtcact ccatttgata gtgaactgtc tacgacggct
    10441 gctaacgttg tcggacgtta ccatgatgca atggaaaaaa tggaattcaa tacagcattg
    10501 gctgaaatct gggtgctgat ttctcgtgca aacaaataca ttgatgaaac agaaccatgg
    10561 gtattagcaa aagacgaaga aaagaaagct gaattgaata gtgtcatgat ccatttagct
    10621 gaatctttgc gtatcgcagc gatattactt cagccaatca tgacagaaac gccagagaag
    10681 atcttcaatc aactaggatt ggactctgaa acaatgaatc ttgaaggatt acactttggc
    10741 gaatttcctt caggaacaaa agtagttgca aaaggtacac caatcttccc acgtttagat
    10801 atggaagaag aagttgcttt tattcaagaa aaaatgtctg aaggtactca aacaaacgaa
    10861 gatacagtca aatgggatcc agaagaaaca gaacttgttt caacgaaaga aaaacaaatc
    10921 aaatttgatg tatttgaaaa agtagaacta aaagtagcag aagtcatcaa ctgtcaaaaa
    10981 gtagaagggg cagataagtt gctgcaattc cgactagatg ctggagacag ccaagaccgt
    11041 caaatcttgt caggaatcgc tgaattttat cctgatccaa gcgaattgat cgggaaaaaa
    11101 gtcgtcattg tagcaaatct gaaaccacga aagatgcgtg gacaaatcag tcaaggaatg
    11161 atcctttctg cagaagcacc agatggctct ttacaagtaa tcgaagcacc aaaatccatg
    11221 ccaaatggct cagaaatcgc atagaaattc aaagggggta tgacactagt tgtgtcatac
    11281 cccctttttc cgaaatttga gaagcaattt tcgtaaattc cttcttatta cttgaagcca
    11341 atcatataat acagaaacag agaactatcc aaaataaatc ctagaaaaag aagtacgagc
    11401 ttcgctgatt tttgttaatt tagaataata ctaataggaa tcgtaaaaac agaaaaaaag
    11461 cataatgata aagggtcggg cagattttaa ttgattcatc tggtactctt ttttattgtc
    11521 cttacattca taaagtttga atgcaatgac tttattacat ctatatttat atctaacagt
    11581 agcgttgatg tgttaattat aagttctatc actttatgtt caattagttt gtgctcttat
    11641 atgtgtgatt gaaaatatag ataaaaaaac aatcatcggc tttgacttcg atgattgttt
    11701 tctttatttt gtaaaaatcc tatcacttga ttttatggta accttttatt tttgtcttca
    11761 aacggcttgg taagagagca gtaagcagtg caaatgggat cgaaaagaca taccagaaaa
    11821 tcgtacgtcc gataccataa tgttgataga gctttgtcaa catcacgtgt aacaagatca
    11881 accctaaaag caaggagcca gctataaacc aatgattatc ccaatgcatg acttcactaa
    11941 gtgccatcac tccaaaagga tagaaataaa aatagatccc gactaaggtc atccgccagt
    12001 tctttgtaaa tgttccgata ttcaataaaa agaaaatcgc tgaaagcaaa ataccgatag
    12061 gcggaaaaag cccaaccaaa aaggaataca acatccccca caagatcatc acaggacctt
    12121 tgatcaatgc aacgattgcg atgaacaaag tcagcaatat tagtttttgt gataacgttg
    12181 caacagtcat taatccaaat aaaattaatg tgaaccaatg ccaatgggac tgctgttgtt
    12241 ctttacggga aaaatcgacc cggttggata aataatctgc taatttcgga taagaacgct
    12301 taaaatccat aaactgttcc tccttcgtgg aaaaggtagg tttagtatac gaaaaataaa
    12361 aaagaaatac atccgtctta agagggagtt tttcactgaa agtggagaaa aaagaatggt
    12421 aaattaaaat attctcagtt atactgaaaa ttataccaaa tttcaaagaa gaaagaggat
    12481 gggaatgaaa ggcaaactaa aacgagaaat caatctgttt ggcgcattag ctaccgttat
    12541 gggaaccgtg atcggtgcag gcgtcttctt caaaactgct gctgttactg ctagtactca
    12601 gtctacaagt ttgacattgc tagcttggct tttgggtggt ttcttaacga tctgtgctgg
    12661 attgacagtg gctgaattag ctacagcaat cccggaaact ggtggagctg taaaatatat
    12721 tgaagctgcg tatggaaaat tgcccagctt tttattagga tgggcgcaaa gcttgattta
    12781 ctttcctgct aatattgctg cattatcgat tatctttgcg acacaattga cgaacttatt
    12841 acaattatct accgattatc ttttgttgat tgcagtgatc accgctgttt ctgtgacagg
    12901 gttgaatttg ttagggacaa aagtcggtgc ttctgtacaa tcagtgacgt tgattgttaa
    12961 attaatacca attgctgtga ttgtcatatg gggattattg accccaggac aaggaactgt
    13021 ccagttattt ccaatcgaag ctggaaaaga tgttactttt gtcgaaggat tgagcagtgc
    13081 gttgttagca acgttgtttg cttatgacgg ctggttaggt gtcggtgcaa tggcagggga
    13141 aatgaaacgg ccggaaaaag atttgcctaa agcaattatt cttggtttga gttttgtaac
    13201 agtcgtttat ctattgatca attttgtttt tttgaaaact ttgccgattg atcatcttgc
    13261 cggtaatttg aatgcagctt ctgaagcatc agacgtcatt tttggaggaa tcggtggaaa
    13321 gctggtcact attgggatct tgatctcggt atacggtgca ttgaacggtt atacactaac
    13381 tgggatccgc gtgccttatg cgatggcttt agaagacgag ctacctttca gtaaacagct
    13441 gacaaagctt tcaaagaagt ttacggttcc ttatgttcct gcggttttcc agttagcagt
    13501 agcttgtatt atgatgagcc ttggttcttt cgacttttta acagacatgt tgatttttgt
    13561 gatgtggcta ttcagtttac tgatttttat cggtgtcttt attttaagaa aaaaagcgcc
    13621 ggaattgcca cgtccatata aagtaccact ttatccaatc gttccgatta ttgcgattct
    13681 cggtgctatt ttcattttag gtatgacgat gatgacgcaa acgaaattag cattgatcgg
    13741 tataggtgtt accttgatag gtatcccagt ctattatcaa aaaaagaaaa aacgctcaga
    13801 agagtaaaat atttcaacaa cagacagaaa aagtacgaac taggtcctct agatcttcgt
    13861 gctttttcta tttcaataaa acaagttctt ctattttgat ttttgctact tctgcaagta
    13921 gtgcttatgt gctaaagtaa gaatgaaaag gagagataaa catgatattt gattctcata
    13981 cccatttaaa tgctgaacag ttcaatgaag atattccaga aacgattcag cgagcacaag
    14041 aaatgggggt aacgaaaatg gcggtcgtgg gcttcgatac gccaacgatt gaaaagtctt
    14101 tgcaattaag ccatgactat ccaaatatat acagtattat cggctggcat ccgacagagg
    14161 ctggcagtta tacgaaagaa atcgaaaata aattacaaga acaattgact ttaccaaagg
    14221 ttgtagcatt aggggaaatt ggcttggatt atcattggat ggaagacccg aaagaagtcc
    14281 aagataaagt atttcgcaga cagattgcta tcgcaaaaga aatgaaccta ccaatcagta
    14341 ttcatactag agaagcactt gaagacacgt atcaaatcct aaaagaagaa gatgttcgag
    14401 atatcggtgg aatcatgcat agtttcagtg gtgattatga atgggcaaaa cgatttcttg
    14461 acttagggat gcatatttca tttagcggtg tcgtcacttt caaaaaagca ttagacgtcc
    14521 aagaggctgc agcaaatgta ccaatcgaac gattacttgt ggaaacagat gccccttatc
    14581 tggcaccagt tccttttaga ggaaagcgaa atgaaccagg ctatactcgt tatacagtag
    14641 aaaagattgc tgaacttcgg aatcttccga cagaagaagt ggctgcccaa acatggacaa
    14701 atgcgcaccg attatttggt ctaagcgaac atggataaga tgaagatcca agagattata
    14761 gtcgttgaag gaaaagacga tacaagaagg ctgcgggaag tagttgatgc agatacgata
    14821 gaaacaatag ggtctgcaat caatgaagag atcctgatgc aaattgaaca tgcccaagag
    14881 actcgtggag tgatcgtttt taccgatcct gatttttcag gagaaaaaat aagaaaaatc
    14941 attatggaag ctgttcctca ggcaaaacat gcttttttgc cgcgcacaca agctaaacca
    15001 aagaaaaagg gaagccttgg tgtcgagcat gcaagtgatg ctgctatttt agaagcattg
    15061 aaaaaggtcg tgacgccaga catttcccaa acggctgttt ctgaaatcac tagacaagac
    15121 cttttagact atggtttaat agctggggca caagcaaaag aaaaacgaga aaaactaggt
    15181 gacgagttgc gcatcggtta cacgaacggc aaacaattgg aaaaacgttt gaagatgttt
    15241 cgtattacgc cgaaagaact cgcccaagca atgaaaatag tggaggaaca acatgagtga
    15301 atatcgtgaa attgcgacac cctctagaac aaaagagatt ttattgaaac atggtttttc
    15361 atttaaaaaa agtttaggac aaaattttct gactgaacca aatattttaa ggaaaatcgt
    15421 agaaactgcg caaatagatg aacatacgaa cgtgatcgaa gttggaccag gaatcggtgc
    15481 attgacggaa caattagcca agcatgcaaa acaagtcgta gcatttgaga tcgatgatcg
    15541 tttgatccct gtattggctg atacgatgca accctatgac aatgtgacta ttgtacatca
    15601 agatatttta aagaccgatt tgtcaacagc tgttagagaa acatttcatg aggagcttcc
    15661 gttaaaagtc gtagcgaatt taccttacta tattacgaca ccgatcatga tgcattttct
    15721 tgaatcagat ttggttgtgg atgaactagt cgtcatgatg caaaaagaag tagctgatcg
    15781 tatctctgct gaaccaggaa cgaaagctta tggttcatta tcgattgctg ttcaatatta
    15841 tatggaagcc agtttagcat tcatcgtacc aaaaacagta tttgtccctc agccaaatgt
    15901 agattcagcg attttgaaac tgacaagaag agatataccg gcagtcgaag tgacagacga
    15961 aaaagcattt ttccgattga caaaagcggc atttcagcaa agaagaaaaa cgctttggaa
    16021 taacttgcag cattcgtatg gcaaagatga ccagacaaaa gcatggttag caaaaagctt
    16081 ggaaacagct ggaatcgatc caaaacgtcg tggggagact ttgtcattac aagaattcgc
    16141 cgcattaagc aatgcgatgt cagaaaatat gcagtaaaaa aagaggttgt gacagaagtt
    16201 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatgt
    16261 ttttcgtttg tttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttaagaaaga
    16321 ggttgtgggc ctgccgttta actctaagtc acaaggaaca atttcaaaaa acagcttctc
    16381 atgtttttga gttagttggt cttattactt gatgcaagct tttgaccact attatgccga
    16441 acaaggagat gtgacacaaa ttttgtcaca tctcctattt ttgtttcttg atcaaaaaat
    16501 tcttatactt gatactcaca ctcattttgt atggatttag aaaagaatat gtgtcttgac
    16561 agttattagt aagtgagtta tactaagtcg acatttatgt gtcaagacat attaggagag
    16621 agaaaaaatg agaaaaaatt cagttcgaaa actaacgatt tctgctttat tgattgggat
    16681 gggggtcatt atcccaatgg tcatgcctaa aatcatgatt ccaccagctt cctttacttt
    16741 ggcgtctcat gtgccattat ttattgcgat gttttttaca ccaggagttg cagtggctgt
    16801 tgcgctaggt acgacatttg gtttcttttt aacaacgccg gttatcatcg cattgcgagc
    16861 attgtcacat cttgtgtttg cgttgattgg ggcttggtac ttgcaaaagc atccgagtat
    16921 cgttttaaaa aatggacagt ttacttttgc taattggcgt ttccaagggt tcaattttgt
    16981 gatcggtgtg attcattctt tagcagaaat gctagtagtc agcatctttt actttattgg
    17041 taatatgcct gaaacatatt attctcaagg atatttttat acagtattca tcttgatggg
    17101 gattggtggg cttatccaca gtttagttga ttataatatt gcttatttta tagcaggaac
    17161 actcagcaaa tattttgata tccccgtatt tacggcagca aaaaaagaaa atcggaaaga
    17221 gtcgattcaa gggtacgcga agaaaacgac atattagaca tgatctctca aaaaaaaaac
    17281 tctcatctag taatggataa taaatcctct aagatgagag ttttttgtcc taaaaagagc
    17341 tgcaccaaca tcttctggca cagctctaac agggggcaat ttatggaagt ttgatgactt
    17401 gttctgtttc tttatcaaag aagtggcctt tgctgatgtt gaaggcaagg tcgactattt
    17461 cgcctggttt atggaaatct cttgcatcta cttttgagat gaactcagta gaaccagttt
    17521 ttgtgtaaag catcgtttca gctccaagta attcggaaac gacaacttct gatttcacta
    17581 cggcttcagg tgaagcttca agggctactt gttcactgtg gatgtcttct ggacgaatac
    17641 cgaagataac ttcttttcct tcataacctt tttcaactaa tactttgttt tttccttcag
    17701 gaatacgaag cttaagccca tgtccatctg tgataacacc atttttcaat gtcacattaa
    17761 agaagttcat ggcaggagat ccaataaagc cggcaacgaa catattgacg ggtgtatcat
    17821 aaacttcttt cggtgtaccg atttgttgga taaatccgtc tttcatgata acgattcggt
    17881 cagccatagt cattgcttct gtctgatcgt gagtaacata gatggtcgtt gtttcaagac
    17941 gctggtgaag tttggcaatc tcagcacgca tggctacacg taatttagca tctaaatttg
    18001 ataatggctc atccattaag aagacttttg catcccgaac gattgcccgt cccaatgcca
    18061 cacgttggcg ttgtccacca gataaagcag ctggttttcg tttcaagtat tctgtcagac
    18121 ctaagatctc tgctgcattg tccacacgtt ttttgatgtc tgctttatca tatttacgta
    18181 atttcaagcc aaaagccata ttatcaaaaa cagtcatatg aggatacaat gcgtagtttt
    18241 ggaaaaccat ggcaatatca cggtctttag gtgccacgtc attcatgact tttccgccga
    18301 tatttagttc tccttcagag atatcttcca gtcccgcgat cattcgcaaa gtagttgact
    18361 ttccacaacc ggaaggacca acaaaaacga taaattcacg gtctttgatt gatagattaa
    18421 aatcagttac agaataattt tctgcattgt catatttttt atatacgttt ttcagagcca
    18481 tttctaccat aatacgttcc acactttctt ttttagtttt attaactatt taaagtataa
    18541 atgaaagcgt taactaactt caatgtcatg ttgaacaaga attttttctt ttttttgtca
    18601 atgtgctagt tttaagcttt aataaatatc cataagaaat aaaaactgat acactggagt
    18661 aaataagtat gttaacaata tcaaaaggag tgtctcttat gaaaatcgct ttgatcgctc
    18721 atgaccgaaa aaaacctatg atggtgaaat tagcaacagc gtataaaaca atattggaaa
    18781 aacatgaact ttttgctacc gggacgactg ggcagaggat cacggaagca accggcttgc
    18841 cgatccatcg gtttaaatcc ggaccgttag gcggggatca gcaaattgga gccatgattt
    18901 cggaggatca gttagatttg gtgatttttc ttcgagaccc gctggctgca cagccccatg
    18961 aaccagatgt aacagcactt atccgtttat ctgatgtcta tgagattccg ttagctacga
    19021 atatcggaac agctgaggtg ctactaagag gactggaagc cggcttccta gattttcgaa
    19081 gaatcgtcca tgaaatagac aagcatccac tttcattttg atggataagc gtatggaaaa
    19141 ataacgttta atgttcgcct atatgtagct ataaaagcta aacaaactga taatatgcga
    19201 tataatacga acgtattgga taatataaac aataatatta gtttttcgta tttgcagtaa
    19261 atgtgaaaat atgctatagt ttctgtggga aaagatccca aaaataatac agagtagaaa
    19321 ataaagcgtt aagtgctaag aggatgggat gttgcctctt ggacgaagga cgtaatgttc
    19381 gcggttttat tttcgcattc gctgcatgat acgatgtagc agctcttaaa ggagatgcta
    19441 aaaatgaaga aacaccggtt agacgcacgt atgattgcta ttatgggctt attgattgca
    19501 ttgatggtca ctttgtcccg actggtagca atcgagaccc catttattaa aatcagtgtc
    19561 acatttattc cacaagtgat catgggcatt ctttttggtc ctttttggtc agggataggc
    19621 gcagttttag ctgatttagt aggaatggcc ctcttctcga aatctgcatt ctttattgga
    19681 tttacattga atgcatttat tgaaggagca atctacggat tctttttcta tcgaaaagag
    19741 atcacttgga aaaatgccat tctggctacg cttagtgtca cgctgatcat caacttattt
    19801 ttgacaccat tatggcttgc gttgatgtac catgtgccat tgttcagctg ggtcgtttgg
    19861 gcgccccgcc tgttaaaaac agtaatatgg ttaccgattc aatcaattgc catttattac
    19921 gtaggacgta gtattcctta caaaaaaatt ttaagaagtt tagcgatcca cgctaaa
//
LOCUS       DACCYH010000053        19836 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.054, whole genome shotgun sequence.
ACCESSION   DACCYH010000053 DACCYH010000000
VERSION     DACCYH010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.054"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(227..1114)
                     /locus_tag="IVY85_001791"
     CDS             complement(227..1114)
                     /locus_tag="IVY85_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8044807.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1111..1980)
                     /locus_tag="IVY85_001792"
     CDS             complement(1111..1980)
                     /locus_tag="IVY85_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8044808.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2117..3403)
                     /locus_tag="IVY85_001793"
     CDS             complement(2117..3403)
                     /locus_tag="IVY85_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8044809.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3676..4254
                     /locus_tag="IVY85_001794"
     CDS             3676..4254
                     /locus_tag="IVY85_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8044810.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4254..5183
                     /locus_tag="IVY85_001795"
     CDS             4254..5183
                     /locus_tag="IVY85_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8044811.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5180..6916
                     /locus_tag="IVY85_001796"
     CDS             5180..6916
                     /locus_tag="IVY85_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8044812.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="IVY85_001797"
     CDS             complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="IVY85_001797"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP8044813.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7812..8321)
                     /locus_tag="IVY85_001798"
     CDS             complement(7812..8321)
                     /locus_tag="IVY85_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP8044814.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="IVY85_001799"
     CDS             complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="IVY85_001799"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP8044815.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9622..10713)
                     /locus_tag="IVY85_001800"
     CDS             complement(9622..10713)
                     /locus_tag="IVY85_001800"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP8044816.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="IVY85_001801"
     CDS             complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="IVY85_001801"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP8044817.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="IVY85_001802"
     CDS             complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="IVY85_001802"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP8044818.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="IVY85_001803"
     CDS             complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="IVY85_001803"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP8044819.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(14676..15650)
                     /locus_tag="IVY85_001804"
     CDS             complement(14676..15650)
                     /locus_tag="IVY85_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP8044820.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(15631..17514)
                     /locus_tag="IVY85_001805"
     CDS             complement(15631..17514)
                     /locus_tag="IVY85_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP8044821.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(17532..19757)
                     /locus_tag="IVY85_001806"
     CDS             complement(17532..19757)
                     /locus_tag="IVY85_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8044822.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
BASE COUNT         5597 a         4319 c         3560 g         6360 t
ORIGIN      
        1 gacaatcagg acagtcatcg gcaatatagc agcaatcact tctttaaaat tttttagcat
       61 gtatcctcct cctttactct ttaagtttac cttaaaatga aagagttttc gagcacaaac
      121 ttaaaacgtt atcatttaat ttttctcaac ataaaataac acacgtccca aaagtatctt
      181 tttacttgga aaaatgactt tgaaggaacg cgcttattgt gtttttttat tttacagagc
      241 cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa tacgatggca gttggtaaag
      301 tagcgatgac taatccagcc atgatcattc catagtctgg tgtataggaa gcacccatag
      361 cagataaaac aagcggaacc gtccgtttat caggagattg caaagctact aagggccata
      421 aataattgtt ccagctactc atgaatgtga tgattgcagc cgcggcatag gtatttttag
      481 ccgtcggcat atacactcta aaaaaaatac ttagttcttt tagaccgtct aatcgagcgg
      541 cttctactag atcttttgga aaagatttaa cattttgtcg aaagaagaag atcaaaaaag
      601 cagtgctaac agatggcagg atgacaacca agaaactgtt gattcccaaa accgttccgt
      661 tcaattgtga aaacattcga tataaaggga tcatcaatgc cgcaaaagga accatcatcg
      721 atagcagtag aatattgaaa attaggtctt ttcgtttact ttgataaatc tcaaatccat
      781 atcctgccat tgacgaaatc agcaatgcaa acacagtggt caccaaagcg atcatcgctg
      841 aattccaaag ggaacgagta aaattcagct cattggaaaa caaattttga aaattgatga
      901 tcaagttact gccaatcttt atttttcccg atgtgatatc cacgggtgta ttcgtcattc
      961 ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga taggatgctt aatgctgtat
     1021 actttaatac tgacccaatt gtcgaacgaa gttctaatgc ttgttttttg acgttggggc
     1081 gttcgattgt ttctttttct accatatttt tcatcgactt tctcctccta ctttcgtttg
     1141 gattatcgac aatatcacaa taaaggaaac aataacaaag gaaactgctg ccgcatatcc
     1201 aaaattaggc gtgaatttat aactcaaatt atagatgtat tgagataggg tggtcgttgc
     1261 atttgctggt cccccgccag taatattttg tacttcatca aaaagctgca atgtcccgat
     1321 cgtcgaagta atggaagtaa acaaaatgat tggttttaaa tttgggatcg tcactttgat
     1381 aaactgctgc catttagaag caccgtcaat agatgctgct tcataaatct ccgaatcaat
     1441 attttgcatg cctgaaagga aaaaaatcat attgtatcca gtccaacgcc aagtgatcga
     1501 aagaatgatc agaactctcg cccaaattgg atgagtcaac cagccaatcg gtgtgtcaat
     1561 cataccaatg cccaacaaca agttgtttac aaggccagat tgagaaaata gacttttcat
     1621 gatcagagaa taactgacca tcgaagtgat acatggaaga aagatagcgg ttcgaaacaa
     1681 ccctttatat ttcaactttt tatcatttaa aaggttggaa ataaaaagtg ctagaaacaa
     1741 catgatcggt acttgtacaa gtaaatacag gaatgtatta aaaaaagctt ttttaaaagt
     1801 tgcatctgtc aacatccgct gataattacc taacccgtta aatgtcatat tgacaggggg
     1861 accactttga aaagacgtga tcaaagccaa aaccatcgga ataaaaacca tgattgatat
     1921 caaaatgact ggcaagatta aaaaagcatg cccgtttttg aataaatctt tctgcttcat
     1981 tcttttccct ccaattctat cattgattat ctacatgaaa agacaagaaa gacaatatct
     2041 gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg atgaaaaagc gaagaaattc
     2101 acttttttac caccgatcaa ttagccaatt gtgcttctac ttgtttttgt gtatctgcta
     2161 gtaccttgtc tgtctcttct ccattgatga ttcgatgaag agcttcagct acgactgctt
     2221 caattgcata ggtctcatgt ccgtaattca cttctggtat ttcagatgtc catgttgaaa
     2281 aatcgtcaaa gatcttttga ccactgtaaa attcagaagc ttcttgataa gcttcttgtt
     2341 cattcgcgct aagcatcgta gatactagtc cgatttcttt cgcaagagtg cccatcagtt
     2401 cttcattcga agcaaacgtt gcttctagga aatctttcgc tgaatcttct ccagctactc
     2461 ctttgatcac ataccagcca gcgccgccaa ggttagaagc ttgcgctttt tgcagatttt
     2521 ctggaagtgc aggtattggt gcaatcttcc attttccaga ttggttttct gctccttgga
     2581 tcgttgaaga ataccaagcg ccagttgggc tagaagctac tgctcctgat tgtatcgcag
     2641 ttactccagc attccagtca gaggtttgtt cgactagatc ttctttcaaa agagtggcaa
     2701 acaattcaag accatacttt aaagatgcat tgtcttggat cgtgactgtc tccccatctt
     2761 cagatgtata ccattctcct gcctgttgca taatcatcct tactcgacca aggtcagaag
     2821 gattgacttc agcgattttc tttcccgtct tttctttgac atctcgcgct acttgaatat
     2881 actcgtccca cttcagattc tccatatcct cttctgtata acctgcttct gccatcagat
     2941 ccgtacgata gaagtttgct gttacgcccg aatcgaaagg aacaccgtat attttatctc
     3001 ctactttgtt taccgcaaat ttatacggtg caaaatgatc ttcctttaca atagcagaca
     3061 aattagcgaa tgcatctgga taagaagtca aatagccttg tatcctataa tcttcaatca
     3121 agacgatatt gggcaatcct tctgtattcc cgctcgctaa agcggtgttc agtttctgaa
     3181 cgatatcatc ctgagacatc gtaacaactt ctatctctgt ttcttcgttc tcataaactt
     3241 tttttgcttc attcactgct ttgatattga atgtctcatc ccacgcccag acagtgactt
     3301 tttcttcttt gtctgctgta ctactagcac ttccgcatcc tgttaagaaa cctgatgctg
     3361 ccgctaaaca aacgactcca gcaattaact ttctgatgtt catttttatc cgctcctttt
     3421 ccatccatta gttgtttacg tttgctacat tcatcatacc aaaatttgaa aacggtttct
     3481 ttattattca gatacttcaa aaatttctcc taaaataata aaaaaataca aatcaactat
     3541 gacaaaagga ataatcttag catctagacc aaagatcgaa atggtgaggt ttctttaaaa
     3601 taaaaatatc cttgcatttt ctcctagaaa taagtatttt taaattacaa gactattctt
     3661 aaggcgggta aaaaaatgcc aatacgcgta attcttatcg atgatgaaca gacgatttta
     3721 gatggaatga aacagttgat caattggtct gagcacgggt ttcaattaat agggactttt
     3781 tccagtccgc tgaaagcgct tgatttttct ttggtacatg caatcgatat cgtggtcaca
     3841 gatgtaatga tgcctgaatt aaatggaatc gaattatcgc ggctattaaa acagcaacaa
     3901 cctgaaacac agatccttat cctctctagc tatgatgaat ttgatatggt taaagattct
     3961 tttaaagaag gcgtcgctga ttatctttta aagcctcgat tgagtcctga tttttttcta
     4021 aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc ccaacaaaag cccaatcacc
     4081 aatcgttatg agcagatctg cgagaatctt tcacagcaga ttgctggaca aactcgtcca
     4141 ttgcttgacc ctcttctttt tgaacacacc ttgtttttct gttatatacc gaaaaacggc
     4201 cattcagttc aaaacaaggc tctaaacaaa aacagtcgat cacccattca ttgatggata
     4261 gcgacgattc gtttactatt tatcctgttt ctaccttttc ggaagaatcc ggctatgtaa
     4321 tcaatactga ttccaaagat acacttagga agtttgttca aaagtttcgt caagaagttt
     4381 ttccaggtga tttttttgtt gtttctgatt cttttgcaat ccaagatatc aatgaaatat
     4441 tccaagagat cagaaattgc accaaaggac aacgtttcta tcataaggga aagaaccttg
     4501 cttatcagca tgaacttctt ccgttattaa ccaaccacga gcagcaagca agtagttatt
     4561 taacaaaaat cgttcataaa gactacgctt tagctgtcaa agaacttgag aatttcttca
     4621 cgacgatatt ggaacagcct attgatccag cttatttgaa acatgaggca atcaatcgtt
     4681 tctatgcatt actcaacgct ttggctgacg agcatgttgg gaatgaagaa ctttctttgc
     4741 tcaagatcgc ccttccttct ttattagccg atgctgagta tttcgaagat ttttctattc
     4801 ttgtcaaaga aacacttgag aaactcaccc acttttctgc aaatgcaaaa aaagacaatt
     4861 tcgatatctt gactttgatc tacgagttcg tccaggaaaa ttatgagaaa gaaatcaatt
     4921 tacaactttt atccgaaaaa tatcattttt catatagcta tctctcagcc atctttactg
     4981 aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt ccgtatcagt aaagccaagc
     5041 tctttttgac ggaaacacgg cttactttga cggaaatctg tgagaaagtc ggctataccg
     5101 aactgggtta tttctccaga gtttttaaag aagagacagg aatgacacct tctcaatacc
     5161 gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag catgaactct ttgccaaact
     5221 tctgttcatt ttgttcatcg gtctttttct gcaagccatc gtcatttctt tgtttattta
     5281 tcatcgatca agggatgcct atatccagct gttcaatcaa tcgaatgatg tggtactaaa
     5341 aaagattcaa tctgaatttg aaagtttgaa tgatacaatc gagaatacat tagccgcttt
     5401 tgattctaat ccagctgtca aaagctattt ttccaatgat ccagcccaac atatggaaca
     5461 gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct ctttcaaaaa tccatccaat
     5521 gatcgattac gatgtattga tctttggaga aaatggaaga acttttgtcg gaaacgacat
     5581 gctcactgcc gtatctgccg attctttttt tcaatctgcg attgctcaga gagtgaatga
     5641 acgtgctgct gatacacaaa tgctttttgc acaccacgga ttgactcttc gcgataagaa
     5701 agcacccagc gtcttctttg tcaaaaaact gaaaaataca ttgaatcacg tttacggata
     5761 tgctgtttta tcgatctcat ccaagcaact agcgaatctt tttcaatctg tagtaaatcc
     5821 tgagatatcg aagatctcct tcatgaacga agaacagacg atcatcgcat caaatgaaga
     5881 aaatacaatt gggagccgat caatgatttt caaaaaacta acagtaggtg aaacaacgat
     5941 ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc caggattgta cattactcag
     6001 tcaagtcgat cttcattctt tatctaacca aatgggcgtg atttttccga ttattttgta
     6061 taatatcgtg acacttgctt ttgctggctc attggtattt atgtatttgg atcgacacac
     6121 aaaatcgatc taccgcttga ttgattcgct aaaacggcta gaggaagatg gcttaaaagc
     6181 tactgttcct gtacaaggaa gccatgaagt ccaattgcta gggaatacga tcaatcaatt
     6241 gctaagtaat gctcagcatc attatgaaca gacgatccaa gacgaaaaga agaaacgttc
     6301 gcttgaaatc caaactatgc aagcacagat acagccccat tttatttata atacgctgac
     6361 tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa gcagttcaag gaatcgacag
     6421 tttcattact ttactaagaa gtacgattgg gaaaaaagaa gaagtgattc cagtcaagga
     6481 agaaatcaaa agcgtacaaa gttacatcga tcttttgagt atgcgatatg gcgaaagaat
     6541 ctcggctaaa ataatgattc ctgatgaact gcagtcatta tctatgccga atatgatcct
     6601 tcaaccaatt atcgaaaacg cctatcttca tgcttttcac aataaaacgc aaggattcat
     6661 cacggtgtac ggcaaaacaa aaaatcagct tcttttattc gaagtgattg ataacggcga
     6721 tggcttcgat actgaaaata ccaaacaaaa aaatgatcat ttttcaggaa tcggcatcag
     6781 ccatgtagat gagcggatac gcctaatgta tggcgatgca tatggcttaa cgatccaatc
     6841 agtgattggg acaggaacga ctgtaacgat ttgtctaccg cttcttccta aaaatcaatc
     6901 agaatatcaa aaataaagaa tagccaagcg ctgatttttt tattttaagt taaataaatc
     6961 aatcagtatt cttgactttc atctacgcgt ctcttctact gtcagcactg gataacagcc
     7021 caatatttga ctttctcctc cgagcatctt gatttcttgg agcgcattct ctactaatgt
     7081 cattggacgg tccaacaaca aatcgatcac gaaaaaatat tctcccaagc tagttttcaa
     7141 tggtcgggat tcaatcttac ttaagttgat ttcccgccag ccaaaagctg ccagcatttt
     7201 atgaagcatc cctggccgat tagcaggtag tgtcaagatg acactcattt ttactggagc
     7261 tgactgctgg ctggtcatct ttcggtcacc gacgatccaa aaacgtgtct ggttcaactc
     7321 attatcttgg atattctcag ctaagatctc caatccaaca tgctgtgctg tttctaatga
     7381 agcaatcgct gctgcgtctt ctttcggatg ttctgctaca tacattgctg cagcagttgt
     7441 tgattctgtt gctactaaag gaacattagg ataatgcgtt tctaaaaact gttgtgattg
     7501 tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt gttgctggat tgcctaaaag
     7561 ctgctgcttg ataggaagaa cgatttcact tttaacttcg acttctggat gtttggaaag
     7621 aagatcgatt gtgtgatgaa cagctccttc caatgaattc tctacaggaa cgacagccag
     7681 atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg atggaagcat acgcagtcaa
     7741 ttgttctgta ggaaatagct gacaagccgc ctgataagtg aatgaacttt ctggccctag
     7801 atagctgacg ttcatatgct tttgatcctt tctataatgg tatcgatgat cttttctggt
     7861 gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt cataccaaga aagtcgttgt
     7921 gccaataatg cttttatttc ttctcttgtt tttccaagag ctaaaggtcg aatagacgtt
     7981 tcatcttgtt ggatacgatc aactagacag tccgcttctg cctctaagta aagaacaatc
     8041 ggctgtgttt ttaaaaaacg ccgattttct gcactttgga caatccctcc accagtcgcg
     8101 ataattttgt cttcatttga cagctttctt aaaatatctg actccacttt tcgaaagctt
     8161 ttttcaccgt aatagtgaaa atattcggca atcgacaatt ggatatgttc ctcaatcttt
     8221 gtatctaaat ctacatactc tttttgtaaa cattgagcta accctttacc gattgttgtt
     8281 tttccagcgc ccatgaaacc aatcagcaaa atgctagcca tgagcttctc ctcctgacac
     8341 aagttctgct aaatcgtcaa aaaatgccgg ataagagatt gaaacagctt cagctccttc
     8401 tagttcgcaa ggtccatcag ctatcaaagc agctacttgc agcatcatac cgattcgatg
     8461 atcgcctctg ctggacactt ttgcgccatg cagaggtgtc ggcccatgga tgatcagacc
     8521 gtctgctgtc gcttttatct ttgctcccat tttctgtaat tcttctgcca ccgcatcaat
     8581 tctgttggtt tcttttactt ttaattcttc tgcgtctttg atgatcgtga ttccttctgc
     8641 ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc agacgcggaa tgatttctcc
     8701 accaatcgtt gctttcttca aatgactcgt cttgacactt aaatcagccg attggttctg
     8761 ttcattgtga ttcgtctggg taatcgctgc gcccatctcc tccaacacat ctaagatacc
     8821 agttcttgtt ggattgaccc cgacattttt taacaacagt tggctttccg gaacaagaag
     8881 tcctgctgct agaaagaaag cagctgatga aatatcacct ggaacagtta tctgttgacc
     8941 agttaatttt tgcggtccgg tcactatgat cgttttatct tccactgtga tccttccgcc
     9001 aaattgacga atcatttctt ccgtatggtt acgggaacgt tctttttcaa cgattacgga
     9061 tgtgccttct gcttgtaatg ccgcaaacag caaagcagat ttgacttgcg cactggctac
     9121 aggcatattg tatcggatag gtgataaaga gcgggttcct gtcacagtga ttggtagata
     9181 ctggtcattt tcttctcctt gcaagtcagc gcccatctcg cgtaaaggtt ccatcactcg
     9241 ccccattgga cgtttagaca acgaagcatc cccgaacaga gtagtcgtaa aaggctgtcc
     9301 agccaaaata cccatcaata aacgtatggt cgtaccggag tttcccatgt caattgattc
     9361 ggttggttgg acaaatgagt ggctaccgcg accatgaatc ttgatcacct cttcctcttc
     9421 ttcgatctcg acacctaatt gttggaaggc atgtaaagta ctcagacaat cttctgcgcg
     9481 caaaaaattt tgtatagtcg tcgttccttc agcaattgct ccaaacatga tgctgcgatg
     9541 ggaaattgat ttatctgctg gtatcctaac agtccctctt aatccatgta tttgttgtaa
     9601 taattgcatt tatccagcct cctatttgat ccagcaatga taactggtat tagacataat
     9661 gattcttttt gcttgtaaca aatcctgttg gtttttaaaa ctgatttgca gtattcctaa
     9721 aatatcttcc ctggtctctt ggatcttcaa attaatcaaa gaaactcccg cttttccgat
     9781 cagtccagtt acttctgcaa taacgccagg ttcatctggc acatcgataa ataaatcaaa
     9841 aaaggcagga atcgcgcctt gcttatggac aggcaactgg tctcgacttt cttttgcccg
     9901 attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg atccattctg ctatttgtgt
     9961 catttcttgt tgccaatctg ccaatcgttc caataatatc tgcttattgc tcaacaaaat
    10021 atctgtccac atgacaggat ctgaggaagc gatcctagtg atatctcgaa agcctcctgc
    10081 tgccagttgt ttggctcgcg gatactcttg attgaattgt tctgcctgat tgactaagcc
    10141 agctgccaga atatgtggaa aatgacttag cattcctgtt atacggtcat gttctcttgc
    10201 tgtaagagtt acatattttg ctttggttcc gcaaaatagt tcttgcagtt tttcaacatc
    10261 ttttttcgtc ttttccaaag gtgtaaagat gtaataggca ttttcaaata gtttagcatc
    10321 tgctgcctgt atacctgatt tatgagaacc agccattgga tgtccaccga caaaccgaag
    10381 atttttctgc tcagcggatg tcacgacttg ctgcttcgta ctgctgacat ccgtaatgat
    10441 gacgtgatca gacaagttga ttctttcaag gacatcaaga taagctaagg ttgtacggac
    10501 gggaacggct aataagataa cgtctgcttg ttctgcacct gtttcaaaat cacttgcaac
    10561 gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt tttgactgac tatcccaacc
    10621 aactactatc agttctggat tggtctgacg aatacaaagt gccatagaag caccaatcag
    10681 tccaagcccc acgataaaaa catgttgtgc catcttattt gccccctttt tagtaatgtt
    10741 tcaagtagtc acggtactcc ttcactgcag ctttcaactc aacaagcgaa tcacttggga
    10801 attttgacaa tagctctgtc gctaccacag tggctacaac attttccgca acgacgctag
    10861 ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc tttgtaaggt tcttttgtat
    10921 cgatatcaac actttgaagt ggcttgtaca atgtaggaat gggtttcatg accccgcgaa
    10981 tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc gcctagatta ttggtacgtc
    11041 ggaaatagcc tctttcttct tcccatagga tctcatccat cacttctgag cctggtaatg
    11101 aaccagaagt gaatccgacc ccaaattcta cgcctttaaa ggcattgata ctaaccacag
    11161 cttgtgcgat tttggcatct agcttcgtgt cccattgtac gtagcttcct aatccagctg
    11221 gaacgcctcc aacgagcact tctacaattc ctccgatcgt atctccattt ttctttgtct
    11281 gatcgatcaa atcttttatt ctttgctcaa ctgctggatc aacaactcgt acttctgacg
    11341 cttcagaaat ttctttgatt tcttgaatcg agatatttcc aggtatttct gcttggattc
    11401 caccaagaca aatcacgtgt cctgctacga tgatgtcaag ttcttccaga attttcttgg
    11461 caattgcgcc gattgctaca cgcatggtcg tctctcttgc agaggaccgt tcaagcacat
    11521 tccgaagatc tcgatgatgg tatttcatac cgcctaccaa atccgcgtgt cctggacgcg
    11581 gacgtgcaac tcgccgcagt gattggtctt tttcaggcac ttctgctaca gacatgacag
    11641 ttgtccaatt tttccaatct ttattttcta ccactaaagt gacaggtgaa ccaattgttt
    11701 tgccgtgacg gatgccagac gtgatccgaa cacgatcttt ttcgatcttc atccttcctc
    11761 ctctgccgta accagcttgt ctgcgagcga gttcgtgatt gatttcttct tctgacagag
    11821 gtaatcctgc tggtagtcct tcaataatgg ctgttaattc tggtccatgg gattctcccg
    11881 cagtcatata acgcatctta cagtcctcct aagtaatctt tgaatgtctc tgtgggaaca
    11941 ggaacgattt ttgcttgccc aatcttttct agcaagatga tattcaattg gcttcctctt
    12001 gctttcttat catgggttat cgcgtggaat aattcatctg gatgacttag atcgtacgta
    12061 atcggcagat ggaatttttg aagtatcctt tctaaagcag ctgtcgatcc agttggagac
    12121 aagccgattt tttctgcatg agaagtgatg gctaccattc caatagctac tccttctcca
    12181 tgactgacta ccccatatcc tgctgtattt tcaattgcat ggccaattgt atgaccaaaa
    12241 tttaaaataa gtcgatttcc ttggtcaaag acatcttctt ccactacttt tcgtttgatt
    12301 tccaacgtag cagtaatgat tggaacagca tgttccagaa gatctttttc gtctttcagc
    12361 tcgttcagct gtttccaaag cgttgcatct gctatagctg ctgatttgat gatttcagcg
    12421 attccttctc gtaaacgacg taccggcaaa gtttttagaa cctcaggatc aatcaataca
    12481 ccagctggct ggctaaatgt tccgacaagg tttttcgctt tgtttgtatt gactgctgtc
    12541 ttcccgccaa tactagaatc tacttgtgca agtagcgtag tcggaatttg aacgaaatgg
    12601 atcccacgca tgaacgtcga ggccacgaat gctgctagat cacccaccat tcctccacca
    12661 agtgcaagta tcccatcgct gcgggtaaaa tcattatctg ctaaaaaatc gtataactca
    12721 gctgcttgtt ctaaagattt actggtctca cccgccggta cgattgaaag gactggttca
    12781 aaattagctt gtctcaattg gaacatgatt ttttctgcat atagtggagc aacgtttgta
    12841 tcagtgatca cagctacttt ttgaggtctc caaatcgttt gaagccatga tccagcctgt
    12901 tctagtgcgc ctttttcaat atgaatatcg tatttttggt tatcaacatg tacagtaagc
    12961 attcgtttca cttctttctt ttaaaaagct ggaattaatc gagtgtctga atcttttcca
    13021 ttgcttcttt catgatcgca acttctttca tcatgcgtga gtaagtcttt tcattcaacg
    13081 attggggacc gtctgaccaa gcatttgcag gatcaggatg gatctctacg atcatgccat
    13141 ctgctccgct tgccacaccc gcacgagcca ttggtggtac aagttcccaa attcctgtac
    13201 catgacttgg atcaacaatc acagggaaat gagttaattt tttcatcaat gggacagcac
    13261 ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg aatcccacgt tcgataaaaa
    13321 tgacaggaga tttgtcttcg actgcaatgt attctgcagc attaagccat tcattgatcg
    13381 taccggaaat tccacgtttc aatccaacag gtttacctgt tttaccgaca gcagatagca
    13441 gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat atcgctgtac tcagccacca
    13501 tatcgatatg cccttcatcc atcacttcag taatcacttt cattccaaat tcatcagccg
    13561 cttgacggat gtatttcaat ccttcttctt ctaaaccttg gaaagcatat ggagaagtac
    13621 gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc ggctttggcc attcgcgcac
    13681 attctctgat ttggtctaat ccttcgatag aacaaggacc agccatcgtc acgaaactgc
    13741 cgtcaccaat ttttactccg tctacatcca cgactgtatt gccaggatgg aattctcgag
    13801 aagtcaattt gtatgtgttt aaaatacgca cagcattttc tactccttca tagcgtgtaa
    13861 atgctccttc ttgtaaactt cgcgtatcac cttttagtcc aatcactact cgttctgttc
    13921 cttgatcgat tgagactttg aatccttctt tttcgatatg atcgacgact ttttttattt
    13981 gttcttccgt tgcttctttt ttcataatta ctatcattgt ttttcccgct ttcttttgtt
    14041 tacttatcta tcttggctga ttttctctac tttttcaagg tggttttaca ttaattccta
    14101 ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc tttcctactt atgacccaga
    14161 taatcaacaa aaaaagatgt ccctgtagga aattgcctct acagtgaaca tcttcaacga
    14221 gccttaaaaa gcgctgcaaa aatataaaat agccactata gatgaatttc tctacgtggc
    14281 tgtaagtcgt gtttaaaaat ctttagccat catagataca aacctggttt gtttgtaccc
    14341 acgaagtcat gaatacaaac ctatctaaaa tagctaaagt aatacgtatt cagttttgta
    14401 agttttctag atttcataaa aaatcgctcc aaactgatgt aagaattgta cctagtatag
    14461 acataaaaaa atcgactgtc aatcattttt atgaaacttt ttttaatttt ctgatagtta
    14521 actttgtttt tcagaaaaat aacaattggt tttattgaag aaaatagtca tgaacaaaag
    14581 tatctgagaa cttttttact ctcttgttca tgacatatag aataaagcag ctttgtcttg
    14641 taaactccgt ttttcaaata ataatctaaa gattgctact gaataataaa gccgatttca
    14701 tggtcctctt cgctgctgat tcggtaaggt gactcaacat ccgctcccca gctattgata
    14761 cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg ttcgtctttc tggcggtagt
    14821 tcttctatat gtgttgcctg ttccagttct tctgctttga atggtaaagc ggaaaaagca
    14881 aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt cttgatcttt atccgtatta
    14941 tccaacgttt gatttcttgt tagttctacc catttcgttt ccatatgcat gccgcattct
    15001 tggggaacaa ggtatgggga aacggggagt cctgccactt tataaatacc ttcgacacca
    15061 ccagccatcc gatccggata tgtttcccct gataggcctt catactcaaa aaattttgct
    15121 ttcgttggca taatgaacct taaacccaat gcgggtaatt ccggtaatcc tttttgtcca
    15181 taataatgca tattgacaag cattttaccc tctccagcga tttggtagga aattgtcact
    15241 tgggtttttg gtactgtgtt gatttccagt gtaaaaatga tttcaaccgt ttcagcgtat
    15301 tcattacttg aatattgatt atttactgga gcaatcggca aatcgatctt tttattgtcc
    15361 accagtactt ctatttcttt gacttttgga tacaatccgg ctcctaacca cataccggat
    15421 ttaaatccaa atccgctacc tctgtcatta tcggtcgttg ctcgccaaaa aactggaagt
    15481 gtttcccggt atagccattc ttttccgttt ttattgagcg attccagtcc gcctcttgta
    15541 taagaaaaaa tataatgcgt attccccgat ttgacaccta atgtacagtc gccgaagacg
    15601 attgcaactt tttccgtatc catatgactt ttatttactg tatttttcat aataatatct
    15661 tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa acgatcccat tccctgagaa
    15721 ttcatagtca cttggtttat cgtcaaagtc accgccatag cgcatcactt ctctgcctgt
    15781 gattggatca gtcacttgga tcgcctgatc tttaaaatcc catatatagc ctccttggta
    15841 catttcataa cgatcaagca acgtcatgta agaatccatt ccacctaacg aattccccat
    15901 gtcatgcata tattcacata agatgaacgg tttttccgct ttattttcaa ggtagtcaat
    15961 gatctcttct ggtggcgcat acatgcagct ttttacatct gaaatcgaat cttcgaatgc
    16021 tggatttgga aaattccctt cataatggac aagtcgatcg gggtctactt ttttgaagta
    16081 atcgttcatt tgtcgaatgt tttcttccgc ataagattca tttccaagag accaaaaaag
    16141 aatacttgga tggtttttta atgtttcgaa attcgttttt gctcgatcaa gaacagccgc
    16201 ctgccactga ggtactgcac ctggtacgtt gtaagaaggc tcaaatgcgc ccattttttg
    16261 ccatgagcca tgggattcga ggttcgtttc cgccatcaca tggattcctg cttggtcgca
    16321 taaataatac caagtgatct ggttggggta atgagatgtc cgtacgccgt tgatctttgc
    16381 attttttatg atagccaaat ctttttccat atcttctata gtgatggcgc gtcctttttt
    16441 cggatgccac tcatgtcgat ttaccccgtt gataatcatc cgttttccat tgaagtagat
    16501 gattttatct ttgatttcga ttttacgaaa accgaaggaa tagtcaatat actccgttac
    16561 tcttttttct tcgtctatcg tctccaattc aagcttatat aaattgggct gaaaatgccc
    16621 ccacagtgcc acattttcaa atccatcgat gcgaatcgtc atttgctctt ttaacggata
    16681 ttgctgttga tagatttcct tcccatcagg atctagtatc gctacattca gctgactgtt
    16741 tttggctact tgctcaaaag acaaatctac ttcaagtttg ccattttgac atttgtcatc
    16801 taagcgaggt ttgatccaaa gatcttctaa atggttttgt cctcgagcaa ctaagaagac
    16861 atctctgaag atcccaaaaa atcggaaaaa gtcctgatct tccaagaatg cagctgtact
    16921 tcttttgtaa acctccacag ctagaatatt ttcttcttcc aaataaggtg ttaaatcaaa
    16981 ttcagacgga gtgaacgaat cttcggcata cccgataaaa tgaccattca accaaataaa
    17041 cattgcttgt tctacaccag caaaatgaat cgagatttgt tttcccttta gaccttcttc
    17101 taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct ttagcagctg aaaagatccc
    17161 ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg cgaaatacat gtccctccca
    17221 cggatacatc gtattgatgt aatggatctt gtcataattt gctaattcaa tatgactagg
    17281 aacagtgatt tcatcaaaag aacaggcatc aaaatctttt ttataaaatg tttttggtcg
    17341 taccgaagga tttggcgaat aacaaaaacg ccatgatcca tttaaagact gtttaaggga
    17401 ggatgtggtt tctacttctt cccttgttgc ataacaaaca tgatcgctat gtgcatttga
    17461 ttggttcact ctaaaaactt gtggatcatc taaccatttt atatttgctt tcatactttt
    17521 ttccttctct ctcataactt tttgaaatga agcagttctg tttggaaatc accttgaaaa
    17581 tcggggaagt aaagtccaac tgtcatcaat tcggcccccg aaaatatccg cttcgaggtc
    17641 tgttcctgat aaagtgcctc atcatctaac cccatcagat atacttcatg gaacgctggt
    17701 tgagctgaag ctagtatcct ccctaaaaac acaatagctt catcttgttt aggagacaca
    17761 aacatccatg cggcaatatt ttcttcaaaa ggactcgcca aacgataata atggccatac
    17821 tggatcaccg gacgaatcgt ttgataggat ataatttggt cttttacttg ctttttttct
    17881 tcaggagaca gctttgtcaa atccagttca taaccaaaaa cagcgctcat agctgtttct
    17941 cctcttgttt tcaacgaagt agatctgtcg gtctgatgat ttggtacagc tgatacatga
    18001 gctgtgatgg acgaaatcgg ataagcaagg cttgttccat attggatttt catacgctcc
    18061 actgcatccg tgttatcact tgtccatgac tgcggcatat aataaataaa cccggcatca
    18121 aaccgtccac ctccaccaga acagccttcc cataagattt ccggataatc agttgtcaaa
    18181 tgttcaagta gttcataaag cccgagcata taacgatgcg ctacttctcc ttgtctctgt
    18241 gggtcaaggg taatcgaata gacatctgaa agcgaacggt tcatatccca tttgatgtag
    18301 tcaagaggaa tcgtatctaa aatctttctc atttgtttct caatcgtctg ccgaacatct
    18361 aaccttgtaa aatccagtac atgttgtgat ctggaagcag aaggcattct acctggctcc
    18421 tgcataagaa aatcaggatg tgttcgatac agttcagaat caaccgagat catttcaggt
    18481 tcaaaccata aaccaaattt caatcctttg ctatgagcat aatctgctat ttcacgtaat
    18541 ccatttgtca acttgccttc atactcgaac cagtctccta atgaagaatt gtccgcatct
    18601 ctttttccaa accagccatc atctaatacg aacatttcga tgcctagctc ctttgcttca
    18661 tcaatgatcg ccttgatttt ttcactattg aagtcaaagt aagtggcttc ccagttattg
    18721 accagaatcg gccgttcagc aaattgatga cgacctcttg cgattctttc acgtagcaac
    18781 tcatggtgga tctgactcat tttgttcaat ccctgacttg agtaagaaag gatcatttca
    18841 ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa aacgatggct gttgatccca
    18901 ccgacgactc tcaattgatt gatctgatct ttttcaagtt cgaaagaatg gttgccggaa
    18961 taaacaaatt gaagtccgat cgcttctcca gtattttctg tcgtatgatg atgaactaaa
    19021 gcaataaagt tattcatatg gtgactgctc gttcctcgac ggctttcaaa tacttttgtt
    19081 ccagataaaa cagattgccg tgaaatctgt ctttcattga catgtgctcc tggcaaagca
    19141 atgacttcat caaatcgcct agtatgggca aagtctagct ggaaactagc cgctttttct
    19201 agtttgattg tttctccggt ttcatttctt atctttacag atcgtgtgat cactgctcta
    19261 tcagtaaata tggtatagaa caaatcgaaa taaatcgctg tttctctatc cttaagtgaa
    19321 atcaccaacg tttgtgcctc tgacttgtcc atcacataag tttgaggcaa gccttcgatc
    19381 tctggcttcc catctgtaat ataatggctg tcatatctca gatccagtaa gtctgatcca
    19441 ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca tgctgccatg agaagagtat
    19501 tcttgcggta gtgtatcttt cgacagagac cggtcaggat ttaagggaac atttcctgaa
    19561 aatccacgat ctcttcgggg ataattttta ttgtctttgt actgcttgac tggtttgcca
    19621 aaataaatat gcgccaatac attgctttct tctatcttta tcagataaga aagaaatgta
    19681 tttcgtaaat ggaagactcg gctcttttca tcaaataaga tgaaattttt atctgttttt
    19741 ctatgcgtaa tatccatcat ttcactccca acaaaacgga aaccgtttac ttttttttat
    19801 aattcctgta tactgtttac taaacaattt tgtcaa
//
LOCUS       DACCYH010000054        18927 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.055, whole genome shotgun sequence.
ACCESSION   DACCYH010000054 DACCYH010000000
VERSION     DACCYH010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.055"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..213
                     /locus_tag="IVY85_001807"
     CDS             <1..213
                     /locus_tag="IVY85_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044823.1"
                     /translation="GEVAASLARKDEHGLRDGIGDVVVTLIILAMQNDMDLYECLNQA
                     YNEIKGRTGKMVDGVFVKSSDLEEAE"
     gene            210..413
                     /locus_tag="IVY85_001808"
     CDS             210..413
                     /locus_tag="IVY85_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAP8044824.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            410..706
                     /locus_tag="IVY85_001809"
     CDS             410..706
                     /locus_tag="IVY85_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044825.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDMELINDK"
     gene            783..1199
                     /locus_tag="IVY85_001810"
     CDS             783..1199
                     /locus_tag="IVY85_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP8044826.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDALIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            2105..2449
                     /locus_tag="IVY85_001811"
     CDS             2105..2449
                     /locus_tag="IVY85_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8044827.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            2454..2735
                     /locus_tag="IVY85_001812"
     CDS             2454..2735
                     /locus_tag="IVY85_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044828.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            2839..3153
                     /locus_tag="IVY85_001813"
     CDS             2839..3153
                     /locus_tag="IVY85_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP8044829.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            3131..4825
                     /locus_tag="IVY85_001814"
     CDS             3131..4825
                     /locus_tag="IVY85_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8044830.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            4845..6023
                     /locus_tag="IVY85_001815"
     CDS             4845..6023
                     /locus_tag="IVY85_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8044831.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            5986..6672
                     /locus_tag="IVY85_001816"
     CDS             5986..6672
                     /locus_tag="IVY85_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP8044832.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            6672..7832
                     /locus_tag="IVY85_001817"
     CDS             6672..7832
                     /locus_tag="IVY85_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8044833.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKEAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            7842..8717
                     /locus_tag="IVY85_001818"
     CDS             7842..8717
                     /locus_tag="IVY85_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044834.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIKIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            8714..9025
                     /locus_tag="IVY85_001819"
     CDS             8714..9025
                     /locus_tag="IVY85_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044835.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            9015..9368
                     /locus_tag="IVY85_001820"
     CDS             9015..9368
                     /locus_tag="IVY85_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8044836.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            9358..9759
                     /locus_tag="IVY85_001821"
     CDS             9358..9759
                     /locus_tag="IVY85_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044837.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            9752..10156
                     /locus_tag="IVY85_001822"
     CDS             9752..10156
                     /locus_tag="IVY85_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044838.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            10168..10773
                     /locus_tag="IVY85_001823"
     CDS             10168..10773
                     /locus_tag="IVY85_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8044839.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            10793..11155
                     /locus_tag="IVY85_001824"
     CDS             10793..11155
                     /locus_tag="IVY85_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044840.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            11158..11340
                     /locus_tag="IVY85_001825"
     CDS             11158..11340
                     /locus_tag="IVY85_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044841.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            11357..14776
                     /locus_tag="IVY85_001826"
     CDS             11357..14776
                     /locus_tag="IVY85_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8044842.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWIQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            14826..15563
                     /locus_tag="IVY85_001827"
     CDS             14826..15563
                     /locus_tag="IVY85_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8044843.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDAEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFSLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            15573..17864
                     /locus_tag="IVY85_001828"
     CDS             15573..17864
                     /locus_tag="IVY85_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044844.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNMIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHETRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVEDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVNNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQDGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYDTAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNKPFLSFGAPNFR
                     YSNASYITDGDGSSLSINGSAGSSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKIA
                     RFEQSTVQVYKNFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDVDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            17888..18766
                     /locus_tag="IVY85_001829"
                     /pseudo
     CDS             17888..18766
                     /locus_tag="IVY85_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737855.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         6648 a         2780 c         4087 g         5412 t
ORIGIN      
        1 ggggaagttg ccgcttctct agctagaaaa gatgaacatg gtttaagaga cggaattgga
       61 gacgtagtag taaccttgat tattttagct atgcaaaatg atatggattt gtacgagtgt
      121 ctgaaccaag catacaacga aatcaaagga cgcacaggga aaatggtaga tggtgtattc
      181 gtgaagtcga gtgatttgga ggaagcggaa tgaacaacag acatcgcaga ataacaaaac
      241 taagaaaaca ggaactgaat gtactaaaga caaagtttga aaaagaatat ggaatttcag
      301 cagaagaaac atataaagtg gcaagtcagt gtgttgctga tgcaagtgat gctattcgta
      361 agtttggaat ttcgatatta agtgatgatc gtaaatggga ggaaaaagaa tgaaactaaa
      421 agacggattt tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa
      481 gaatcctaaa acacgtaaga aaccaaaagt caaagtcggt gacatggtcc gctgtgaagc
      541 agaggagttc atttatccat ttcgtggata tgtagaacac gtctataatc actcagcgat
      601 cattcgcatt gaaaacacga tggaatgtga taaatggaca gctaaaagca aagagaattt
      661 agcagtagtg agattggtgg atatggaact aatcaatgac aaataaaaaa gccggatcgc
      721 tccgactaac ataataaaac agacaagttt attatatcac ataaaaggag cagtttgact
      781 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
      841 tgaagaactt tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
      901 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagag gatgcattga
      961 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggctctta
     1021 gtctgattag tcgtcagata ctctactata gcttctgtga cgtaaataag cactctaatt
     1081 atgaaatagg tcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcta
     1141 tcgcgctgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttgat
     1201 tttgtagggt ttttgtaggg atagtgtagg gtttttgagt gttttaacgt gatattatga
     1261 tagtgtcgaa agattaggaa acaggatcga caaaataaaa tgtaagggag gaaatctccc
     1321 tcatcgttta attaagcttc gatagacagc agcggaaata ttaagaataa ggatgtgaat
     1381 tttaactcct tctaaattgt tcttattatc tatcatccgt tgctgtctat tgtcattatg
     1441 tcactgtggc ggaaagggta gacgcttaaa aataaggtca atacgtcgag ggatagcctt
     1501 aacgttttat gatttgacca tgcaatgttc gattcattgc cagtgactta aggaacctac
     1561 ggaaacaatt catcttatcg gatgccgatg aattggctga ctagtcggga tgctactagc
     1621 agttagaagg cataaaatac tagcgcagac gtgcgccact ctcaggtgta ggttaggaga
     1681 gaaacattag ttggggttat taggaatacg ataacctgct tgcgacaaag ctttgtactg
     1741 tcgcgttggt catgaacaga gacggtattc tgtttcagta ttcgttagca accgagggat
     1801 gtggcagtgg tgaggtgcag gaagtatcag acttgtttgt gtgcaagtta ctattacata
     1861 ttagatcact ctttgagtgg tctttttatt ttgcgtaaag gaggcacgac gatgtataga
     1921 ccacaatact tagaacagaa gcatgaagta atcactgtgc aaaacggtaa cggtgagata
     1981 gtacgaaagt atagaagacc aataaagagc gatacatata aacggaagga aagcaatgaa
     2041 gttattccat tgtatggcaa aagaatagct aagtattaaa taagattgcg aaaggagatg
     2101 ggacatgacc gaggaattct atagatggtt attacagttg ataagagaag atcgtttggt
     2161 taagttctat cagtctccta aatggcgcag gcttagagag aaagcgatga aacgagatca
     2221 ctatgaatgc caagagtgta gaagactagg taagtatcat agagtagaga acgttcatca
     2281 tataaaggaa gtcaaggata gacctgactt agctttagat ttagataatc ttatttgttt
     2341 atgtgttgaa catcataatg aagttcatgg cagatatctt acagcgttag ataaacaaga
     2401 gaagaagata gaaagcttcg ctaacttcga tgcaagtgaa aggtggtaag tgcatgatca
     2461 tcaatgacaa tggcagagag tatgatacag aaaagattga agagtattca tcttatactc
     2521 agggattaat taaacgtttg atatacgttc gctatgtagg tattagggat ctgttatcag
     2581 ataactgttg tagtaaatac aaagtgaatc aagtaagaga agcgttgaat aaagataata
     2641 acgtcgaaag aataaaaaat gtttttggat atagtattga agagattaat tattacattg
     2701 acttcgctga agctttcatt ccgatggtga gataaccccc cccttaaaat aaatcgcaaa
     2761 ttttttgggg gtgatgaaac ggagggggct gtcaggaaaa gagatttttt cgaactttat
     2821 catgaaagga gggctaaaat gtttaaaaac gaattgtctc aaaatcgcta cagagaaaaa
     2881 ttacgccgct ctttaataag ccaattggaa agtcagaaaa caaatattga gccattctta
     2941 gataatgttg atcgttatat cagtttatgg gaaacggcga tatcactgga agaagatata
     3001 tccgagaacg gcattagact ggagaatggt aaaaagaatg aatcagtagc gttgcttgtt
     3061 tctgtcaaca aacaaatggg attgatgttg gataaacttg ccattactcc tgaattggta
     3121 ggtgaagcaa atgaatcaat tcctgagtta taagcatatt gaaaattggt tcaaagctat
     3181 agaagaaggc actatcaagg tatgcaaaga gcaattattg ctaaaaaatt atctagaaga
     3241 aagagtcttt actagagaag atatttactt cgataagcag atggtagagg attcaatcaa
     3301 tataccagca caatactttc cattcgaatt aattccgtgg gaaaaatttc tacaatgttt
     3361 tatttatggt gttcgatgga aaaaagataa aacactagtg ttcaatagat atctttcatt
     3421 aatgggacgt ggtaatggta aaactggttt tgcttcttgg aacaacttct ttctactaac
     3481 cgctaaacac ggtattaaaa attatgatat tgatatctat gccaataatg aaagccaagc
     3541 aaagactagt tttgatgatg tatttaaagt aattaaagat catcctgatt tagataaaaa
     3601 agtatttaaa gctacgaagg aagttattca aaatatcgct acaaacagca aacttcgtta
     3661 taacacggca aatgctagaa caaaagatgg gaagcgacca ggtgcaaacc gctttgatga
     3721 aattcacgaa aatgaagatt attcaatgat aaatgtggct acttctggtg gtggtaaaat
     3781 tcgagattat agagaatttt atgatacaac taatggtcat gttcgtggtg gtccgcttga
     3841 tgacattata gaagaatcaa aaatgattct ttctggagaa cttggaattg acaaggatgg
     3901 agcagaattt tctagtttgt ttccatttat ttgtcgcttg gataacgata atgaagttga
     3961 tgatcccgac atgtgggaaa aagcttgtcc aactattaat tacaatgcag atttaaaacg
     4021 gaaaatgttt caagaatact ctcaaatgca acgtaatgct ggtttaagac ttacgttcat
     4081 gaccaaacga atgaacagac ctatggaaga tacacgattt gctgttgctt catatgatga
     4141 tgttctgcat acgaaagaaa aagaatttcc tgaaaaaatg gatgaagtga taggaacagt
     4201 cgattttgct gatagacgag attttgccag cgttgggttg ctaggaaaat acgataaaga
     4261 tgtttatttt acacaacata cttttatcca cgaatcagcc cttcgattac aaaacatcaa
     4321 acgagaggtt atagatattt ctatagatca aggaaaatca cagatcgttc atggaaaaaa
     4381 tatagaagct gattatattg taggttggtt tcttgaaatg agtaataaat attatattaa
     4441 aaaaatcgct atggatatgt accgtgcaaa aatattgaag cccgctttag aagaagcagg
     4501 ttttactgtg gaaattgttc gaagcggatc tgttacacat ggtatgttaa aagatctggt
     4561 tgatgacctt tttattaatc aacgtttatt ttttggtgac gatgcgatta tgcgttggta
     4621 ttgcatgaat gtatatgaag agcatatttc taatggaaat atacgctatg aaaaaataga
     4681 acctgaaact agaaaaacgg atggcttttt ttcattcctt catggtttga attttttaga
     4741 tgatatttat gattctgctc ctgtaacagt cacaaatagc tcagtagaaa atacaggaac
     4801 tggatttact cctctagtat tctaacttga aaggaggtga gaaagtgggg atttttcaaa
     4861 aggcggtagg atacttcaca aaaaaagcaa cggttccttt agaagaatac ttttgtaaat
     4921 tgcaagttga ttttgtgtat cgaaaatttg cgattgaaac ttgtattgat ttgattgcaa
     4981 atgcgatgag taaagcggaa ttcaagtcat atgaagatgg aaaaaataaa aagaatgatc
     5041 tttactatag gctgaatgta gctcctaata agaaaaataa tgcaacagaa tttagaaaaa
     5101 aactgatcag gagattaata ttctacaatg aagtattgat cgtttctccg tctaataatt
     5161 ctagcgaaat atttattgcg gatagttggg atgtcacaga atatgcattg aaagatgatg
     5221 tgttttctca agtgcaaatt aacaacatag tccttgatag agaatttcta gaaagtgatg
     5281 ttatctatat aaaatacgca gatcaacaaa ttaggcaact agtcgatgcg tattatcaag
     5341 cgtatgggaa actcatttct agtgccatga atgtttacaa gcgctctaac gctcgtagat
     5401 acgtactgaa agggaattta ttccgaccgc aagacaatac aacacaagat caaatcaata
     5461 aaatgatgac atcacaattt aaggctttta tggaagctga taatgcaggt gcggtatttc
     5521 aattacaaaa tgagtacaca ttagaagatt tcagcggaaa cttccaaagc aattcaagag
     5581 atataaaaaa cttaatagac gacatctttg agatgacagc agcagcgttt cacgttccga
     5641 aaaacctact aaagggagac atgagtgggt tatcggatca agtggacgct tttttaatgt
     5701 tcgaaatcat accgattgct gaacttattc aggatgcgtt taacgctagt ctctatgaag
     5761 tagaagaata cttgtcaggg aattttgtac gtgtggatac aactatgatc aagattacta
     5821 gcttcaaaga tttggttgac gctattgatg taggcattag aaatggagta tttacaatca
     5881 acgaaggaag agagcgcgtt ggaaatgatc gctctgataa ggcgatggca gatgaaatat
     5941 ttataactaa aaacaatcaa caagtatcga aaggaggtga ggcgaatgac gacaatgaaa
     6001 acatttctag cagtaaagaa tgaaggcgca gtaccgcaaa tttttattca gggatttatt
     6061 ggttctagtt ggttctttga agggaatact gacaagggaa tcaaaaatat tttggatagt
     6121 ctaggtgatc aagaagaaat tgaagtagta attaattcaa acggtggaga cgtatttcaa
     6181 gggattgcta ttgggaactt acttaagtca aataaagcaa aagttaacgt tgtgattaac
     6241 ggcttagccg ctagtgctgc ttcaattatc gcaatggctg gcgatactat aaaaatctac
     6301 aacaatgcac aattgatgat tcaccgcgct tccacatacg gagaaggaaa tgtcgatgac
     6361 ttccgtacga ttgctgacca actggaatca attgataaat cggtaaaggc ttcatataaa
     6421 acacgattca atggcacaga tgaagcattg caagaacttc ttgaaaaaga atcgtttatg
     6481 gatgcagaaa cagctttgag ttatggattg gtcgatgaaa ttatcgatgc agaaaatagc
     6541 tcaggtactg aagctaaaaa agaacaaagc gttgaagaaa ttttgaatga cgttaaagaa
     6601 aaaagagcag aaaaaattgc tgcatttaca gcagcattaa ataaaacatt tggacaagga
     6661 gatgtaaaat aatgacagtt aaaaatttaa aaggtgtaac agctgcaagc gaccaattga
     6721 tgaaagcttt taaagatggt aacgaagaat cttttagcgc agctatggta agcttatcta
     6781 aggaaattca ggataaaatt ttagaagaag caacagcaaa aaatcaagat caattagtat
     6841 taatgaaccg tggtcagcgt gtattaacta cacaagaaac aaaattctat aacgaagtag
     6901 tgaaaaacga aggttttgca ggggtcgaag aattagtgcc agctactgta tttgaacgtg
     6961 tatttgaaga tttagaacaa tctcatccac tattgcaaaa aattactttt gttaacacaa
     7021 ctggtgtaac agaatggatt gtgtcacgtg gagtcaatcc agcatggtgg ggtaaacttt
     7081 gcgaagctgt taaaaaagtt ttagataatg gctttgatgt aattaacatg aagcagttca
     7141 agctatcagg ttatattcct gtatgtaagg caatgcttga tttaggtcca gtatggttag
     7201 atcgttatgt ccgtactgtt ttagtagaat cattgagaat tgcattagaa caagcaattg
     7261 ttgatggtac tggtaaagat atgccagtcg gaatgatgcg tgacatgagc aaacaaacta
     7321 gcggagaata tgctgaaaaa gaagcagaac ctattacagc tttagatgct gtaactatgg
     7381 gcggtttgat ggcgcgacta tcaaaattca atatcgaagg tgtgaatgat ccgatttatc
     7441 gtaatgtgaa tccttctgat gtggtcctaa ttgtgaatcc aactgattac tggtctaaag
     7501 tattcccagc taagactgta ctaactgcta atggagaata cgtacaagta ttgccagtac
     7561 cagtttcaga tttgcagtca acggctgtgc cagaaggaaa agcagttatt ggggtagcct
     7621 cagattactt catgggtgta ggatctacac taaaaattga agcttcagat gaataccatt
     7681 ttgttgaaga cgaacgcatt tatctagcta aacaatatgc aaacggtcaa cctaaacgta
     7741 acgatagttt cattgtatta gatattagcg ctttgggaac tactactaca actacaaaac
     7801 caacaaccac aacaactaca acacaagcgt aggtgatcag aatgaagtat attctttgtc
     7861 agccggcaat caatcggttt aaatgggagc ttgaagtttg tttaactaat ctgaagaaac
     7921 taggaatcaa agatatcgta ttgcttttca gcagacacga tgatcagatt cctatttttt
     7981 ttgagaagga atatggtgtt gaagttcatg tgtacgatga tctgcgggac gacaaagagt
     8041 atattccttc gattaaacca tatttatggt ggaaatattt agaagaagat cattcgcgtg
     8101 aggacgaccg atatttctat atcgattcgg atgtcatttt caataaaaga attaatttgc
     8161 gcaaattgcc ttctaaagat gatgtttggt attgtagcga ctgctgtagt tatctaagtc
     8221 ttgattatat tagaagctgt gaaaacggag aaaatattct aaaagatatg gcaaacattg
     8281 taaatgttac agtagaatct ttggaaacta taaacactaa ttcaggaggc gcacagtggg
     8341 ttattaaccg ccctaaagcg aattattgga aaaaggttta tctggattct aatcggctat
     8401 atcgctacct tagagggcaa aaaacaaata taaaaatctg gacagccgag atgtgggcac
     8461 agctttggaa catgatgtat ttcaatattg gtcctaaagt tcacgaggaa ttagactttt
     8521 gttttgctac tgatccaata gaaaaagtta aagaagtaaa aatcttacac aatgctggag
     8581 taacaacaaa cgatgaagat ttatttttca aagggagata cgtgacttct acgccttttg
     8641 atgaagattt atcatttgta aacaagaaaa aatgctctta cgcatatgtt aaagcaatta
     8701 aggcggtggt tagatgacgc ctgaacaagt gactgaagaa ttgctaatag ctgtgaagga
     8761 taatatttac gttacctgga acgaagaaga tgagtcaatt aaaaagatga tagctaaaaa
     8821 tgctgtttat cttcaaagta aagtgagtac aacactttct ttttctcctg aaagcttaga
     8881 atacggattg ctaatcgaaa gatgtagata cgactggaat cgtgctttag atgagtttga
     8941 acaaaatttc gctagtgagt tattaggttt cattcaacat tatgcgctac aagaatatat
     9001 tgcaggtgat gtgaatggcg aataatcgta gactcgaaga aacattcaac gatggttggt
     9061 taaagatttt gacgcaaact accaaaagaa atgaactagg aaaaaagatt ggtgtagaag
     9121 atacagaaat cacttcttta aaatttagaa atctttccat gagagatagt gatataacag
     9181 ctatggatgc gatgggatcg aaattaacta agaaagtaaa gactccattt catccaatcg
     9241 ccaagaaatt taataaagat caatatttta tcgtaatcga tagtatgcgt tacaacgtta
     9301 tctatgccga ttacgataat ttttatatct atttttatct tgaaagtgtg ggtgaatatg
     9361 gtgattgata attctaaaga aaaagaacgt ttaaataagc aaatttctgc tatcaaaact
     9421 tccttagaag agcattttgg cctcaaactc tttcaagact ccgtaggcga ggatgagcta
     9481 cctgatgatt ttaattactt cattctcgaa acaggagaaa tagaaatgat cactgagcca
     9541 aaatatagcg tgggtcaaaa tctatatcta actttctatt cagaaaatag agaagattta
     9601 acaggagatt cactagatat tatttcattg attcaaaatc gttcgattcg ttttcagaga
     9661 atggatccca atcatttaaa actagagaac caagatcgct atatcgatca attggtattt
     9721 acgtttagac gattattgaa gagtgattgt catggctaaa aatagttggg agctaaaaat
     9781 aaatggacat gatgaacttc ttgtgcggat ggaacgctat tcaagcgaga gcgaacgact
     9841 gattaacgaa gcattgaaat caaaaggttc ggctattgca gtggatagga ttacagaaaa
     9901 aattcctgtt tctgaagcag atttaagaag agggcaccaa cacgcaaaaa atagtcgtcc
     9961 acttaagact caatacatta atttgggttt catcattaga cctacaagaa aatttgagta
    10021 tttaaaatat cctgatttgg ggataggtac ttctaaaaga aatcagccgg acgaatttat
    10081 gagaagagga ttaggtcttg cacttgatcc aattacagaa cttctgattc gtcaattcga
    10141 taaattaaat aaataggagg aacaacaatg gctaaaacaa caacagtaac aacttttgac
    10201 aatataagta ttaaaagaat ttcttttaat tttaagaatg caacaaatgc aatttcaact
    10261 gattgtaatg gacaattaga tggcgaaaca gaaatgcaga gaattgtaaa aaaatgcggt
    10321 tcaacagaag taaaatcgaa atctaaacca atcaatatga cggtaacaat tactgcacat
    10381 gtaccgatgg aagtttatcg acgcttcaat gggttgaaac aagatgaacg tattaaatcg
    10441 ggaatttact cttatggtcc tgattccgta ggggaagatt tctctcttgc tgcagagatc
    10501 gtggatgatt tcgaagaaaa aaataagctg attggtatgt tagcatgcac ttcgaataca
    10561 ggattaacat tctctattga aaatggtgcg gatgaagtag ctgcgttaga actagaaaca
    10621 aaagttatgc aagatgaatt tggtaaattt tatcatgaag caattgttgc agaacttgaa
    10681 gaagacttaa cagatcaatg gatgacgaat ctatctgctg atgtgattaa aaaaacttca
    10741 gttgtgacaa ctacggccac tcaatcacag taaaaaaaac ggaggtagcg aaatgaacga
    10801 agattactca aaaattgaac taaacgatgg aacaattttg aatttagaac ctaaactgaa
    10861 tatcaagaaa ttattgatga tcaatagaga ttttaacaca gacgagtttg caaaaatgac
    10921 tgtgggaaaa ggatccatgg atatttctgt tattcaaggt gcaaaggctg tgtatattgc
    10981 ttaccgccaa gcgaacatga ctgattatat ttcattcgat gaatttatcg ataaatggga
    11041 ttttgatatg gctactgcca gctatattta tcaattgatg atgttcaaac aagcacgcga
    11101 tgcttatcaa aaagaatttg aaaaagcaaa taaggaaaaa aagcttcaaa agtaaaaatg
    11161 ccaaagctct tagttgaaac gtgggtcgat gtctattcga tgttgaccga cgttttttct
    11221 atgccttcag atttggtttt aagcgatatc tgtttagatg acattttgca aatggcttac
    11281 aacaagagtg cttatgaagg atggaaaaac tatgcaataa accaatccca gaaaaactaa
    11341 agaaaggagg taaaaaatgg ctaaaaagag aacagaagca gaagtaactt tcatagctaa
    11401 cgatgacgga ttgaaatcta cgttaaaaga aatcagtgct gaattaacta aaaatagagc
    11461 agaattaaaa ctagaacaag ctcaattaca acagactggt tctgaatcag acaagttagg
    11521 aagtaaatta tcttctttag aaaagcagta tgaattacaa agtcaaaaag ttgaagtaac
    11581 tagccaacgt ttagccaatg cgaaaaaata ttatggagaa aattccaccg aagttcagaa
    11641 acttgaaaga gaactgatta atcaacaaac agcgcaacaa cgtttgtcaa acgaaattga
    11701 taaaacgagt aatgcactag ctcaagcaaa aggcgaaata cagacgtacg agtctacaat
    11761 gcaacagttg gatagcgaac aaaaaaatgt tcaagctagt gcttctctga ttgaatccga
    11821 atacaaaaaa tggcaagcaa ctgctggtca atcagcttct gaagccgaga aattagcgaa
    11881 agcccaagaa tatgtttctc aacaatctga aaatgcggag aaaacgatag atatcctaag
    11941 acgacagtta gaagctacac agtctgaatt tggcgctaca tccacagaag caatgcagat
    12001 ggaagcgaag cttaatgatg ctgaacgtga atttgaagag ttaggacaag ctgctaaaaa
    12061 tgtagataca actaacttgg acgatatcgg aagcaaaata gatatgaata atttaatgga
    12121 agcttctgac gttttaagcg acattggcga taagcttaca gaattaggga aacaagcagt
    12181 ggactctgct aacagtgtag gtagttccca gagtaagata caagctaatt ttggtttgtc
    12241 taaacaagag gctgaagaat taacgaatgt agccagagac atttattata aaggttttgg
    12301 agaatcgtta gatcagtcca cagatgcatt gattttggta aagcgtaatt taggagattt
    12361 aaataatcaa gatttacaaa atatcacgga acaagctatg gtcctagaaa acaccatggg
    12421 cgctgatatg gatgaaacgt tacgtggtgt aaatggctta atggtcaatt tcggcttgag
    12481 tgctcaagat gcaatggatt taatggtttc gggtactcaa aacggtttag ataaaacgca
    12541 cgaattaggc gacaatatgg cagaatatag ccaattatgg agtcaaatgg gatattcagc
    12601 tgatgaaacg ttcggaatgc ttcaaaatgg tttagatgcg ggtgcttata accttgataa
    12661 agtcaatgac ttagttaagg aaatgggaat atcgttaaca gatggtcgat ttgagcaaaa
    12721 catggatatg tttagtgaaa gtactagaaa agcttttgaa gagtggaaaa atggcggagg
    12781 aacacaaaaa gacgttatta attccatgat tcaagatttt agcaatatgg atggtcaata
    12841 cgaccaatta aataaagctt ctacaatttg gtctgcactt ggcgaagata acgcgatgaa
    12901 agttgtccaa tctttgactg atgttaatca tacatttgat gatgttagtg gatctgcaca
    12961 aaaaatgaat gaagattcta ctaccccgtt gcaagagttg aacggtaaaa tagctgaatt
    13021 aaaggattca ttagctccta taggcaacac aatcatagat gcactcgaac cagtaattga
    13081 ttttctagga aagatggctg atgcgtttaa taatcttcca caaccagtac aggattatgc
    13141 cgtagcgatt ggcggattga ctgctgcatt tactttatta atgccaataa tagttggctt
    13201 catggctcta ggtggtccta ctacattaat aataggagca gttattactg ctattgctgg
    13261 agttatagca attataaaaa actggggcgc aattactgac tggtttaagg gaatatggag
    13321 taaattcact gattggttgg gtggtacttg ggaaagtata aaagaaggtg cctcatcagt
    13381 ttgggatgga gtcaaagaaa cctggtctgg atttgtagat tggattcaag atatttggca
    13441 aggcgtttct gattggtttg gagagctgtg gagcggatta gttgaaggag cttccaacat
    13501 ctggcaagga gtccaagaga cttggcaaac attcgttgat tgggtttcaa atatttggaa
    13561 cggagtcaaa gaagtatggt cgattatttg ggcagatatt gtagggattg ttcaaatacc
    13621 atggacctta ataacgtcat tgattcaagc tggtattaat attatagtgg gtatttttga
    13681 tgtagctgga cagttattag gcgcagcttg gcaagctgtt tggacaccta tttctgattt
    13741 ccttaaaaac acttgggata ctatgacaca atgggtaagc atcgcttgga atggaattgt
    13801 aactacattc catactatat ttgatccagt tgtggcatgg tggaacggta tatggacatc
    13861 cattagtact acggcttcaa atatttggaa ttcaattagt gcaacagctt ctagtatttg
    13921 gaacagtatc aagaatacaa tcactagttt ggtacaagca gctgctacag taattcaaaa
    13981 tatttggtca actgtatcta gttggttagg tggaatttgg aattcaatca gctctacagc
    14041 atcaaatatc tggaatagtg tgactagtag tataagcaat gctataaacg cagctaaaag
    14101 tgccattcaa agtgtttgga atagtatatc ttcgtggatc agcggaattt ggaacggtat
    14161 caaaaacact gctttgaatc tttggaatgg aattacaagc actattagct ctaaagtaaa
    14221 cgatggaaaa aatgcaattt caagcggttg gtccaatcta acaggtattg tttccgacat
    14281 attcaataat gttaaaagta caattgctaa catttgggaa ggtatcaaaa agactgttag
    14341 cgctccgatt gattggatta gagataaaat cagtggtatt tttgataatt tgaatatttc
    14401 tataccacat attccgttac cacattttaa attgagtggg gaattcaatc cattgaaggg
    14461 aaaaatccca acgttgggtg ttgattggta tgcgaaaggt agtgtgttta attctccgaa
    14521 tattatcggt gtaggtgaag caggacctga agcagtttta ccattgaaaa gatctgtgct
    14581 gcaagaaatt ggtgatcgta tcttgagtag cacatcagtt tcatctaggg cacaaacgat
    14641 tcaacctgtg aacaattacg aattcaattt cacaattgat ggtaacgcag atgaggttac
    14701 tatgaagcaa acaactcaac aaatcattga cagcattaca aaagttcaaa atgataatgc
    14761 ttcggcatgg cgttaaacag gagagtattt ctcctgtttt ttagtattaa aaaggatgtg
    14821 aaaaaatgac tgattgtata cattctataa tcgatggatt tcctgattat ttgcataaat
    14881 tggctttagc tgaaagacca accataccat ctccaaaaag acagagagtt gaaacttctg
    14941 ttttaggacg gttaggtggc ttagtacaag attattcgtt tgaagatatg tcgtttactt
    15001 tgcactataa ctatttagag gatgcggaag accatcaagc gttcaagcaa tcgttttata
    15061 tcatgcgtca ttggttaaac tatgcaaaga aattagaatt ctctgatgat cccaacgtct
    15121 attatgttat ccagactatc gatattgggg atgcagaaaa cgatattgtt gaatggggag
    15181 agttcgatgt aaatattact gcgaaaccat tcgcaagagt tcaagaagat gtgcctataa
    15241 ccgtagataa accacagtca tttagtttgc tgaataatag tttagaagaa agttttccaa
    15301 agattatcat cactccttca gctacttcat gccagttcat cttaaatgat tatgtgttta
    15361 gttttgaagg cttagtagta ggaactgatg tagtcattga tagtgattta atgctttgct
    15421 acgaagagca atcggacgga gatattttag atcgatccaa caaaatgaag accatgcaat
    15481 atccgacatt gcaagtggat attaattatt ttaattgtac tggtttgagc aaaatacaaa
    15541 tttatcgtaa tgggttaagg taggtgaaat agatgatcga taatttaata actatttacg
    15601 ataaaaatga cgcgaataat ttagctgaac atttatatga tacacaaggt ttaggcgctt
    15661 tgtcagactg gttaacagcc actgttagca ataaattaaa cggagccgag atatttcagg
    15721 gtacttatcc aataagcgga actaatgcag acttgattgt agacggacgt attattcagt
    15781 gctatgtgga tgaaaatcga gcaaagcaac gtttacggat ttattatgca aagacttctg
    15841 taataggaaa tatgatagaa gtaaaagctg aacctatttt caatgatata agaaaatcgg
    15901 tgttgaataa atatgacagc ggaacagaaa agatcactgc tactcaggca tggcaaaacg
    15961 caaaagtttt agcaaaacca gctattcctt cgcagttttc tttctcatcg ttagtagata
    16021 cgcttgctaa tgtgaagata gaaaaggcga attttttaga attctttggc ggaaaggaag
    16081 gatctatcct agatcgattt catggggaat ttcttaaaga taataacaca ttacgtcatg
    16141 aaacaaggct aggtacggat cataaaatca aagcgattta tactaaaaac ttaactggtc
    16201 tcgacttaga gatagatgct caaagtgttt tagttggagt ttatccattc attagcagtt
    16261 cgtcagaagg agaggatgag atcactctac cagaagaagt cattttcacg gattacgtgg
    16321 aagattatcc tgctggatat gtttcttttg ttgattttaa agacaaagcg actgatgtgg
    16381 ccacattaag ggaagctgct aaagactggt tgaaaacaaa catagataaa cagaaaccac
    16441 aagtgagtgg ttcgattgaa ttagtaccat tgaggcatca aagaggctat gaaaaatttg
    16501 ttgatctaga aaaagtttcg atgggtgacg gagtagatgt gtatcatcca cagttaaaag
    16561 tgaatatgtc agcaagaatt gtggaatata cgtttaatgt tttaaccaat tcatacgata
    16621 aattagttgt aggaaacgtc aaaacaaact tcttagaaaa tacagagaat aatgtcaaca
    16681 atttgattaa tgatgccatt gatcaattga aaaatggtgg cgaaatcagt gatttaatca
    16741 atgatattgt agatcatcaa actgacatga ttactggtca agatggtggg tatgttttac
    16801 tagatcctaa agaagcacct agtcgtattt tgattatgga cacgccagat aagaataccg
    16861 caaggaatgt tttacaaatc aacaatgctg gtattggttt ctctaaaact ggtattaatg
    16921 gaacgtatga caccgcatgg acgttagatg gcggatttaa tgcctcgttt attacagctg
    16981 gtgagatagt agggattact attagaggta ctacattaat tagtgatggt actgattata
    17041 gaacaagtat tgccaatggc aaaatgactt ggtactcaaa aaaagttaac aaagatatta
    17101 tggagctaga agcacgtgat tatgtaagtg ctgatgccgg tattgtatca tacaccatga
    17161 aaactggtgg tggtttcatg attagaaatc cacaggggaa tttggttttt agtacgtggg
    17221 ataatggcaa taacaaaccg tttctatctt ttggtgcgcc caatttcagg tatagcaatg
    17281 ctagttatat aactgatggc gacggtagtt ctttaagcat taatggtagt gcgggtagtt
    17341 catgggagtt taaagtagct ggcaggacta tgaaatttac tagtgatggt atgctaacgt
    17401 tgccaggttg tttttttggt tcatgggaag atgggaaaat tgcaaggttt gaacaatcaa
    17461 cggtacaagt atataaaaat tttactgtta gaggtactaa aaactcaacc gtaccgacag
    17521 aacattatgg acaacgacta ttgaacgctt atgaaactcc agaatattat ttcgctgatt
    17581 atggagaagc cgttacaggt gatgatggta aagttcgtgt tgatattgat cccatgtttg
    17641 ctgagacagt aaatctaagt cggtatatga cacatgtgac acctacagaa ctagttttgt
    17701 gtgctgttac tcatgaagat gttgaccatt tcatcattga aactagtaag ccaaacgtat
    17761 tagttagatg gaatttagtg gcacaccgtc tagggtatga agatattaga ttaaaagagg
    17821 atacagcata tgatagcaca gtgcttgacc aaaaacgttt ttaaaacgaa gacaaggagg
    17881 tatataaatg gctagcagtt tatataattt ggctttagat ttcagcaaag aattaaacta
    17941 caccaaagct attatggctc gtcaaggtga taaagggatt acggtgacgg ttaaaccgtt
    18001 tctaaatggc ttgcagatgg atacgagtgg cggaacattt actttaaaag gaacaacacc
    18061 atctaaccgt tacgtagata atgttgcaac tagtgtaact agtgaagaag tcacgttttc
    18121 tcttgatggc acatttatga gtgaagcagg atattataaa cactgctacg tagaatatag
    18181 aaaagacaat caaattttaa caacgcaaga tatcattttt ttctcactag gagtgtctga
    18241 catttcgcaa ggccaagccg atgaatatgt ttcgcaatta gaagagttga ttcgaaagta
    18301 caacgaaact tttgatgctt ttctggctga aattaaaggt agaatggata gcttaaataa
    18361 acagattact gatttaactg gtcaagctaa aacactacaa gacaagttag atgctctgaa
    18421 agaagaaatt tctaaattag gtaacttaca tgtgatgtac tccaacagca tcgatttcgg
    18481 ggaatatgat tattcgggga atccgaacct tagcgctaaa cttaatgctt caagtttttc
    18541 tttaggtact ggcgcaacag tcgcggatga taatgatgag attgtgttta ccttagatgg
    18601 cacaaatcaa ctatcgaagt acgctagcaa agcgcaagtg ccggtagcag aagggaaaca
    18661 atatacgatt agttgtgaaa ttatgttaga agatgggttc actggcgacc cttcaggaat
    18721 acgtctgcaa cacgcatatt tgccaggcgg ggttgttgtt ttacagacag agacagtacc
    18781 taaaaatgaa ttgaatacgt ggcagacgct tatcggaacg cgaacggtaa aatacacctc
    18841 tgacgtacca aatgaatggt accctttgtt tagagacatc cgaacactta aaccgaccgg
    18901 taaagttaga ctaagaaaaa ttaagat
//
LOCUS       DACCYH010000055        18597 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.056, whole genome shotgun sequence.
ACCESSION   DACCYH010000055 DACCYH010000000
VERSION     DACCYH010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.056"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(201..1070)
                     /locus_tag="IVY85_001830"
     CDS             complement(201..1070)
                     /locus_tag="IVY85_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8044845.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(1122..2114)
                     /locus_tag="IVY85_001831"
     CDS             complement(1122..2114)
                     /locus_tag="IVY85_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8044846.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            complement(2291..3775)
                     /locus_tag="IVY85_001832"
     CDS             complement(2291..3775)
                     /locus_tag="IVY85_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP8044847.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            complement(3776..5383)
                     /locus_tag="IVY85_001833"
     CDS             complement(3776..5383)
                     /locus_tag="IVY85_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8044848.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            complement(5432..6316)
                     /locus_tag="IVY85_001834"
     CDS             complement(5432..6316)
                     /locus_tag="IVY85_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8044849.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            complement(6332..7324)
                     /locus_tag="IVY85_001835"
     CDS             complement(6332..7324)
                     /locus_tag="IVY85_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8044850.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            complement(7948..8394)
                     /locus_tag="IVY85_001836"
     CDS             complement(7948..8394)
                     /locus_tag="IVY85_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP8044851.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            8989..9339
                     /locus_tag="IVY85_001837"
     CDS             8989..9339
                     /locus_tag="IVY85_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044852.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            9544..10392
                     /locus_tag="IVY85_001838"
     CDS             9544..10392
                     /locus_tag="IVY85_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAP8044853.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            10439..10633
                     /locus_tag="IVY85_001839"
     CDS             10439..10633
                     /locus_tag="IVY85_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8044854.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            10636..11838
                     /locus_tag="IVY85_001840"
     CDS             10636..11838
                     /locus_tag="IVY85_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8044855.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            12064..12258
                     /locus_tag="IVY85_001841"
     CDS             12064..12258
                     /locus_tag="IVY85_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044856.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            12248..12601
                     /gene="tnpB"
                     /locus_tag="IVY85_001842"
     CDS             12248..12601
                     /gene="tnpB"
                     /locus_tag="IVY85_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP8044857.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            12703..14250
                     /locus_tag="IVY85_001843"
     CDS             12703..14250
                     /locus_tag="IVY85_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP8044858.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            14402..14830
                     /locus_tag="IVY85_001844"
     CDS             14402..14830
                     /locus_tag="IVY85_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8044859.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            14817..15059
                     /locus_tag="IVY85_001845"
     CDS             14817..15059
                     /locus_tag="IVY85_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8044860.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(15359..15574)
                     /locus_tag="IVY85_001846"
     CDS             complement(15359..15574)
                     /locus_tag="IVY85_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044861.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            15681..15995
                     /locus_tag="IVY85_001847"
     CDS             15681..15995
                     /locus_tag="IVY85_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8044862.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            16099..17277
                     /locus_tag="IVY85_001848"
     CDS             16099..17277
                     /locus_tag="IVY85_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8044863.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            17680..17973
                     /locus_tag="IVY85_001849"
     CDS             17680..17973
                     /locus_tag="IVY85_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP8044864.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         6065 a         3542 c         3264 g         5726 t
ORIGIN      
        1 atatcagatt tttcgattta aaagatctgg taaaaagaac tttcttgtac tcaatatcat
       61 ttcgtaatta tattaatttg aaacagaata aaagtctcta ctcagattct caagaaagaa
      121 gaatgaattg agactattta agtttcagaa taccctttta aacttccggt atgttatgaa
      181 tagttctaat aaatactatt ctaatgattg agtttcttta ttgctaaagc taggcagcca
      241 atcaagcccg cattgtcagc aagccttgga gttagtagat attcttcgag agtaggcgta
      301 tccacgtagg aacccatttt ttctttaaac gattggcgaa tcaactcgag tagttgcggc
      361 tgtttcatta cacccccgcc taaaattatt acttccggtg catatagtaa cgttgtgtta
      421 tacacacact gagcaagata atctgcctca atcgtccaat aaggatgatc aaatgagata
      481 gtctgggctt ttcttccaaa gcgtttttca atcgcaggtc ctgatgctaa cccttccaaa
      541 caatcatgat gaaaaggaca gattccttca aattgatctt cattatgacg ttgtaagagc
      601 atatgtccca tttcaggatg actaaaacct gcgactattt tcccatccac tacagcacca
      661 ccaccaacac ctgtaccaac ggtgtaatac agaacactat ttttaccttt tccataccct
      721 gccatatatt ctccataaca agcagcattt acatcggtag tccaatagat gggaaccgac
      781 aaggcgtttt ccaaagctcc tacaaaatga taattttgcc aagctaactt cggtgtgttg
      841 gtgatatatc cgtaagttct tgattcgatt tgaatatcaa taggcccaaa tgctcctaca
      901 ccaattgatg cgatatttcc tttgtactta tcaaagaaat caaacacttg ctccatcgtt
      961 tcttctggtg ttgtggtagg aaaacttttc cgttctatca cttgtaattg ttcattacca
     1021 atcccacaga caaactttgt tccaccggct tctattccac catataacat tttatctaca
     1081 cctttcattc actcattttc aaaaaaagaa acgtatttgc actaagcaaa atcgtttctc
     1141 actaaaggtt tcgttgtgtc accttcgcca aattcaatcg gcaatacggt acgtttttct
     1201 actgtctctc cattaatgac agcaattaat tttgccactg ccacttccgc catttgcttt
     1261 gtaggttggg caatcgttga gactagataa ggatctcctt ctgtcatctt ttgaccatca
     1321 aatccgataa cttggacatc ctctggtacg tttttcccta ttttttcaag catatcaatg
     1381 acagctaatg ccattggatc gttgatcgta aaaatcccat cgatatcggg attctctaac
     1441 aaaaaatttg ttacacgttc tgagagatgg tctaaaggtt ctaacatatc taacgcttta
     1501 taggaaatcc ccagttgacg gcactcgatt tcaaaatatt ttcgacgact cttcgtttca
     1561 ttcggtgttt cttggtaccc accgatatat gctacattct gacatcctcg gcgataaagt
     1621 tcatttgcag ccaaacgtcc accggccgca ttgtcagcgg tcacataaat cacctcttca
     1681 gtgaaataac ggtcaatact aacaaaaggc agattactcg aaatgtattc atcgatatct
     1741 gaataagtaa ttccaatgat gccgtctact ttattttggc tgaccatctg gatatattct
     1801 tgttcttttt cactattacc actggagtta cacaaataaa gcttgtagtt ttgtctttct
     1861 aatgctgttt ctacatggta ggcaaattcg ccaaaaaatg gatgccatac cgtgggtaga
     1921 atcaaagcaa ccgtgttcgt atgcttcatc tttaaccccc gggcataaaa atctggttga
     1981 tagtttaact ctgtcattgc tgcttcaact ttttctctag tagaagcttt aacagaaccg
     2041 ttattcaata cccgggaaac agttcctact ccaacacctg ctaattttgc gacatctttc
     2101 attgttgctt tcatagttga tccctcacat ttctgcacaa gtttagtata atgaattttg
     2161 tcataaatta ctaatttttt caaaatacta atagcaaatc catgggaaat ctagctatct
     2221 gttcttttct aaaaacatta tccttcaaaa cctattaaat aattgattca tcgcctaagc
     2281 aatcaaacat ttatttcaca ctcgctgttt caaaagcttg aatacgactt tctccgatcg
     2341 aatgaaacaa gatatctcga gctttttcca gctcatagaa agtagccgtc ataacttcta
     2401 acccattaat aaagacttca attgatgagg tatctctaaa aatctcaaga acgagctgat
     2461 tatcaatcaa aggtacttca acttttcgta cattcagagg gtcttcttcc gcccctgtaa
     2521 gctctcttcc tagtttctga cgatccaatt caaaaacctg tgtagcagtg tcataggcta
     2581 gtttgagaca atccgtctta gaccgcagta attcaattgt aaaaacttca gctttctcca
     2641 aatctgcgac tagatgtaaa tatgaagaat cagacagcgc gtttcgaaat acgactgatg
     2701 tgtcagtcac tgcaatattt tcactgcctt gttgataaac taaatgacta tatacagttg
     2761 agatgggttt ttgtaccaat cgattttttt tcactcttaa ctctcggggc aaactcatag
     2821 agcctgccca aagatggcct aactcatgtg taggcaatgt acgatcccac atctgcatcc
     2881 aagcaatcat cacccgtcca ccattaggat ctcttgttgt ttgtggtgca taaaaatcca
     2941 ttccaaaatc catttcatgg aaattatcaa ctatcatctg acctgtttcc cagtttacct
     3001 caccaataca agccatggta gaactgatat tatgatattc cagtccgttc ttcttgattt
     3061 gtatcggaga catgattaag acatctcgtc cgtctaaatg gaataaatca ggacattccc
     3121 acattattcc ttgctctgaa gtcccttcta gcagcacaga gtaaaagtgc caagtataca
     3181 aatctgacga ttgaaacatc actattctac cagtagactg tgtagattta gtagctacta
     3241 ccatatagta accatcgttg tgaacaatta ctttaggatc acgaaattct tgtattagcc
     3301 cattttcacc tagctgtttt tctgctacca caggattacc actgtatttg ctaaaatgaa
     3361 taccatcttc agagaccgcc aaacattgat gctgatggct aataccatca atttcttgac
     3421 agcctgtata aaataaatat agttttccgt ctctttcaat tgcacttcct gagaaacacc
     3481 cgccgctgtc ataaggttga tctggggcta acgctactgg tagatcttcc caatggacca
     3541 aatcactact gcgcgcatgc ccccaatgca tgggccccca tcgactatcg taaggatagt
     3601 attgatagaa gagatggtaa tgccctttgt aatatataaa cccattgggg tcatttatcc
     3661 acccgatggg tgcggaaaaa tgatagtttt gtcgatactg agggttaact tttcctcgca
     3721 tcatttcaat aaatcgattt gctttatcca aaatcgccgt ttcttctact aacacttatt
     3781 gtgctccctt caaaaaatta tcatagccag tttgttttat ttgaatccat tcttccaaac
     3841 ctacacgttc caattcagca aggtattctt cccattcttc atcgatttta ccgccggtga
     3901 tccactctgc tcttttgcga tagatgtaat cccacatatc cgtttcgatt tttgctaatc
     3961 gatctgtttc ttctttttct aaaaagacac gtggatagat attgtcttct gtaatatatg
     4021 gcaaatacgt gtcatgcatc aaatctaaac gccacttcgc atcatctggc atagtagtga
     4081 ctgtgccgta ataagaatca agaactgcta atggtcctcc agcttcggtt ttttgtctta
     4141 gttcccccgg tgctgtcccc tctaaaggca aatgttttaa cataccatcc tttaattcaa
     4201 aaatattcgc ctgtgcttca tcaccgtagg ttccccagtt attttggaca gactgtaacg
     4261 gttcatacat tttatctatc cacctagctg taagttccaa gtttttattc gcactagtaa
     4321 ttacgaaacg atctcttgag aaacccatgc cattcgtacg agtaacatgt ttcgttccat
     4381 ctggtcccgc taagactggc aagacgtcat aactatcatc tgcacccgtg atatttgctt
     4441 tatcccaagt aaagtacacg ccatacttat gttctttccc tttggcaaca tacgtattcc
     4501 aatcttgttc gaaagcctct ttatcaataa gacctttatt attcatttca ttgaaatatt
     4561 tgatggcttc tttataccct tctttgttag cagtaaattg tacctgacca tcgttatcaa
     4621 cgactaagtg gtcatcatta tcacctagac cgaatgcccc cattaaaaat tttaagtctt
     4681 cgtttcctcc attgttaata aagctaaaag gaatttcatc tgcttcacca ttaccatttg
     4741 gatcttgcgt cttgaaagcc tccaacacag tcatcaactg atcagtggtt tgcggcattt
     4801 caagacccaa attatccaac cactccttgt taatccaagc catcccattt actgtatgaa
     4861 tcgactcttt tccttcgcct aattcttcaa tccaaggcat agagtaaatg tgaccatcag
     4921 gtgcagtaga catttgtcgg tattcggggt tttcatcaaa aattttcttc agatttggca
     4981 tataatcttc gatcaaatct tcaatcggta caataacgcc actttccgcc cagtttaata
     5041 aatcatagtc acctgcagcg gcgttgaaga tggcatctgg taaatcacca ctagcgatat
     5101 ccaaatttct tttttcggca aaatcactac tgtagtttgt ccaatcaata tggagattgg
     5161 tctcttcttc taatcgttgg aaaatcaatt tgtcattagg atcattggga gccaaagggg
     5221 cactttgaga catcattttt aaagatactt tttctttcaa tggaaaagag acattttcta
     5281 gttcataatc agggcttgat ttactctttt cttttgagcc tccacaagcc cccaaaatta
     5341 gtacggaagt aaaaacgatt ggcagcatcc attttgcttt catcttaaaa tctcctttat
     5401 aatttttatt catacaaata gtgaacaata cttacccttt aacagaacca actaaaacac
     5461 ctttatcaaa atatttttgg aagaaaggat acattatgat taatggcaga ctagaaatga
     5521 cgatcgttgc gtatttgatc aactcagcaa tttgtttcat ttctgccatg gcagtattgg
     5581 ctcccaccat gtcttgtgaa ggttggtttt ggatcaaaat atttcttaat acgagttgca
     5641 acggctgtaa gtcgggatct ttgatgtaaa tcatggcatc aaagtatgag ttccattgcc
     5701 caacaaaggc atataaaaac aatacgaaca taattggttt cgctaatgga agcatgactt
     5761 tgaacagtat ttgcaagtca ttggcgccat cgatagtagc agcttcagaa agttcttttg
     5821 gcaaagcact aaaataggta cgggctaaga taatattcca cacatttata gcacctggta
     5881 cgataatcgc ccatacacta tctaacatgc ccaagttctt taccaataaa taggtaggta
     5941 tcaagccacc acccacgaac attgtaatga caaaaaaagt cataataatc tttttccccg
     6001 ccaagttagg tcgagacaaa ggatacgcag tgatcatcgt aataaataca gtcaataagc
     6061 tgaagctaaa agagtaaaac aacgaattta caaaaccgcg taaaatggaa tcatctgcaa
     6121 atacccgttg atatccctgt attgtccaat ctttcggatt gaagcttata cctttattga
     6181 tcaaagtagc tgggtccata aaagaagcta ccagtacata aatcagcgga acaatggtga
     6241 tgacgactaa aaaagttaca atcactcgat tcaaccataa gatttgacgg tcgatttttg
     6301 ataaagcact attcataatt ttctcctttc attacaagcc ttgaccatcg tttagccgct
     6361 tggttatatt gttgacaatc agtaataaaa cgacattaat aatcatattg aacagaccaa
     6421 cagcagcaga gtaggcataa tcacctgatt gcaagccgac tttgtataca taagtggaaa
     6481 tgatttctga ggcagggatg ttcattgaag tttgcatcaa gtaggctttt tcatatccta
     6541 cattcataat accgccggcg gccaagatga agctgatcac catcacaggc ctcaatgcag
     6601 gcaaatcgat atgccaaatc ttttgtaaaa tatttgcccc atcgatattt gctgcctcat
     6661 ataaatcggt actgactccc gccagcgttg cggtgtaaag aatggatgac cagccgattc
     6721 cttgccaaat tcccgataaa atataaatcg ttctgaaata agctggatta gacatgaaca
     6781 taattggttg ccccgtaacc ttttctacta acccattaat aggtccattt gcggatagga
     6841 acaaaaacag catccccaca ataataacta ctgagataaa attcggcgca tatagaatca
     6901 actggatttt ctgttttatt tttttattga taatttgatt caagctcagt gctaaaataa
     6961 ttggtggaaa aaaacctaac aataaaccat aaatactaat ttttaatgtg ttgctcagca
     7021 aaagcgtaaa atttggtgaa ctgataaatt ccctaaagtg atctagtcct acccaaggac
     7081 tttccaaaat tcctagcata ggattatagt ctttaaatgc aatcacagca ccgtacattg
     7141 gcacatactt aaagataaac gtcatgatga tacctggtaa gagcaacaag tacatccact
     7201 tatcacgccg caactgtttc tttaattttt cccacttcga aatatttttt ttatcccgtt
     7261 gtaccgccct tatctcacca cctggtccca atgtcttttc caattcaatc tcctcccaat
     7321 acattttaga aacgtttcca ttttcgttta aaaaaataaa aatttaacga agatgcattt
     7381 tggaaacgtt accactattt tcgtttatag attaccatat ccattttttt aagtcaatat
     7441 tttttttact ttgtgccaga attttgtatc taagtattct gataataata ttttaaacaa
     7501 cagattattt ttaagcaccc ttttctgcgg cctcattgat agctggaata tatacagatt
     7561 ctagtcgata gataaaaaca gattaagtac gaggtttcca atgaattata aaatccaata
     7621 aaactcagta aaatgtgatt taaaagtatg ttcaagacca ctcaccagta agtttaatct
     7681 atttagcaaa agaagctatg tctacactct ttaaaatcaa tatgttttct catttttaaa
     7741 gtttcaaaaa aactctataa ctaagaaaaa tcacaaaaaa gagtacattt tctagtgaaa
     7801 ctgcaccctt ttttgctttg tttacgtaca aaaaatctct tgagtactta acttctacag
     7861 tataatcaac aaccccttta tagattactg atgttatgct gaaaaaaatt ttttcaccaa
     7921 cttgtaatct aagccacttt tcttatttta cttctcaaac caatcataaa attcatctgg
     7981 tgttttctcc cgatagagtt tgattaacgc tggtcgcatc gattgaaata tttcgactaa
     8041 ttcttcccca ccaaatcgag tggaaaaatg cttatcagta aatatcattg ataaagaaga
     8101 aagaaacagc tccgtatctt catctgtacc aagaatattg atcaactttc caatcaaata
     8161 ggaatcatta aatgtcaggt cttgtttcac atcaaaggtt tttattgatt ctttcaccat
     8221 tttcgcaatg cgatctttct ctccatactt gatctcacca ctcatctcga tatagtcaat
     8281 gatcccagat gaaagaatcg tgacagcttc ttcttttgct tttgatgata gtttgtttag
     8341 cgttttggat tcttcaagta cctctttcgt aatctgtacg acatagtcct tcatgatttc
     8401 actcgaatat tctacaaaac attcttggat aaattcctcc aaaggatagc ctagctcatt
     8461 ctcccaagca ggatttttga actcgcccgt ataaaattct tgctttattt tctcaatgat
     8521 ttcaggataa tcgtccacta tttttttctg accaatgaga aacaaatact ctcggatata
     8581 ttcttccaca tgcccaaaat aaaaccagcc gacaggtttt ccgctgatac tagataattg
     8641 gttgatcaca ctcttaggag ccaacgcata gccttgaacg taggaactga tcgtcggctt
     8701 ttttaaacct aatcgattcc ctaattctgt aaaagagaga gacattccgt cttttatttc
     8761 tttgattcgt tggcccacct tcactttatt aggttgtact aaaccaaaca tattcaaatt
     8821 cctccttatc atttttttca ttataacact cgtataaaaa atcgtatata tttttttaga
     8881 aaatctcttg actacgtata agttctcgta tataatacaa gtcgtagatg gccatctata
     8941 ttttttaatc aaatcgtata aactttcgta taaaaaagga gaattgtgat gccaataaaa
     9001 attaccgatc tcagtttacc aaaagaaaca ctcgaaaaag aataccaagt cgtcaatgtc
     9061 actcgctggc aaaaggaggg cgaaattcta ggttggagct acgaatgtat cctaccaaaa
     9121 cttcgctacg aaaaaatcag tgtcaaagtc aaatctgaac atcctgtgat cacgccggaa
     9181 gaacttaccc aacaaggttt agccataatt acatttacca atttggtgat tgtaccttgg
     9241 ggacgctcga atggacaatt tgtcagttat gggttatcgg caaccgctga tacggcaact
     9301 ttgattccca gaaaacaagg taccactaca caaaactaac tatactatgg agtgttgggt
     9361 ggttaggacg aaacggcggg gcagcgccca cagagccgtt tctgtccgtt aagaccgccc
     9421 aacaccccat aggctagaag ggctgctacg caggccttcc cccttaactt gatttataga
     9481 tcaacatcag gagaaaatga ctggttcttt gacaatcgaa tacgcaacaa ggagtgatga
     9541 tttttgcaag cttataatca gaaaatcatt gaaactccca cgtatattga aatttatgat
     9601 tatgaagcaa tcattactcc caaacgtaaa acgatcgaaa acgaagtaaa tctggaacga
     9661 aaacaaaaat tagcttggat ttatgaaaat gatattactc ttgaaaaaag aaaaaactat
     9721 gatgaattat ccgctcaagg acaatatgac agtttaaaac gcaaacagaa acattatcaa
     9781 agtatgcgat ttgagattgc ccgccttgta gacacgaatt ttgacaatca aacgaaattt
     9841 ttaactctca ctttcaaaga aaacattcaa gacattgagt atgcaaataa tgaatttaaa
     9901 aaatttatca aacgactgaa ttatgacctc tataaaacta aaaaagcaaa tttaaagtat
     9961 ctcgccacgt gggaaaagca gcaacgcggt gcaatccact accatattat cttgtttgat
    10021 ttccccttta tctcatttaa ccggttaact acgatttggg gtcagggttt cgttaagatc
    10081 aacaagatcg atgtagacag tatcgaaaat cgcgggcgct atatcagcaa atatttcgat
    10141 aaagagctgg aattgaaaga gcataagaaa aaagcttttt tcaagtcccg gaatctcaaa
    10201 ctaccaactg tcaccaaaaa gatggtaaag aaaccctatg attccacgaa tcaagatata
    10261 ctatatacaa gcgaatatat agttagacgt ccattttttt acaatgcttt agctttaaat
    10321 gagttgggaa gtcctgaaca agtgatggat tttcaagaaa gtaccgttca ttatacaaaa
    10381 ataaagaaat aacactatta cccaatgata gtgttttctt tgaaagtgag tttatacaat
    10441 gaaaaataca atttgtcgaa aagatttaga ggctctagga tttaaaacac atcaagctac
    10501 cacaataatc cgacaagcaa aaacaagaat ggtaaatcag ggatatccat attataataa
    10561 taaaagagtt ggggtagtac cccgacaagt aattgaagaa attataggtg ttcctctttc
    10621 acaggaggaa taactatgaa aacaaaatac ccttgtgtgt atcaagataa aaatggtcgc
    10681 ttttcttatc tagttgagtt aggaacagat caagtgactg gaaagaggat ccagaagaaa
    10741 ggaacaaaag attctctagg aaaacgattc tcatcagctc gtgaagcaca caaagaagtc
    10801 atgcgtataa aaaatgaata catggttgta aatggatttg cgaattatga tttaacttac
    10861 aaagaattta tggagcagtc ttacgttcca cactacaaag ctagcgtaca aaaaagtact
    10921 tggagttctc gtgagtgtgg attgattcag attactgaat ttttcggtga taagaagctt
    10981 cgtgaaatca atgtgcgaga ttgtgaaaat tatcgaattt ggttattgaa cgatagcgga
    11041 tactcacaag catacagttc acttatgtac ggtatgtttc gtaaatcatt agactatgcg
    11101 gtcaccctgc aatttctgaa tgagaatatt tccaaaagaa caaaagcaat tccaaaagga
    11161 aaatcaatcg ttccttactg gacaaaagaa gaatttgaag aagttttggc aaaaatttat
    11221 aaagatgatt tctacgaaca tatgtgtttt ttaatgatct ggctatatta catgactggt
    11281 gttcgtgtaa gcgaaggatt agcacttaat tggaatgacg tggacttcaa acgtaaaaag
    11341 ttgagggttc atcacacctt ggaaatgaca aatcaaaaag attttattcg gaaaccttac
    11401 acaaaaactg aaagcggctt gcggatcatt tctctggacg atgatacaat tgaagtgcta
    11461 aaaaagtgga aagttattca aaaaaatcat ggtgtagaac aatttattct tagctacact
    11521 gacctcccac tctatcgttc aactgttcaa cgaattattc agcgttatgc caaactagct
    11581 aaggtacccc taatacaagg aaaaggatta cgtcattctc atgttagcta cctaatcaat
    11641 gagtttaacg cagacattct ggtcgtgtcg caacggttag gccattctag ccctgaaatc
    11701 actttaaagc actacgctca tctatggagc cgtaatgatg aaggaattgc agaacaaatg
    11761 acaggaaaca ttaaatttac ctgtgcaaca aaatctaaag ttgcaaactt taatggaaat
    11821 caagcagtaa aaatctaacc ctaccatttc cctaccaaaa tgaggaatag gctgaaaaag
    11881 cactgatata atagcttctt tacgaggcta ctcatattga gtgggaataa gacgtaaata
    11941 taatatggat ggggaatgcc gataaatcaa cgtttttcaa cgtaagcgcc ccatagaaca
    12001 gtaccttact aacaaatgca cctcaagata tacttgagct atcaagaagt ctggaggttt
    12061 tctatgtata aattaaatga gattgttcct gtccaattag gaaacgaaaa gagtgcacca
    12121 gaaaaaggta aacccgcggc tgccaagtta gcgatgcgca tcaaaagaga gaatggttct
    12181 gaagttttga tctataacgg tattaacaat tatattcttc aagctatttt gaaagaactg
    12241 ttgtccaatg ctttatgatt tacttcaaac caaacatatt tatattgtgt gcggaaaaac
    12301 agatttgcga aaaggaattg atggtcttgc cagtcttatt caacaagagt atcaactcga
    12361 gctttacgaa gatgccgttt ttttattttg cgggaaccgt caagaccgtt tcaaacttct
    12421 ctattgggac ggtgatggtt ttcttctctg ttataagcgg atagaaaacg gcaagttaaa
    12481 atggcctcgg acgaaagacg aagttcgaac cttaacgaac caacaggttc gatggttgtt
    12541 agaaggatta agtatcgatc aacctcgagc tattttgcca ggaaaaaaag gagtttttta
    12601 agctgggggg ttaacacaaa aaatgtgttg aacccctatt tcttgtgcgt ttcttgtggt
    12661 attctgtaac aaaatcagtt cgattcaagg tggtgaaaaa ttttggaaac tacagatacg
    12721 ttgcttcagt tgcttcaaga agcccataaa accaatcaag cacaacaaca aaccattcag
    12781 aatcttacaa ctgaaatcca actattgaat gaaaaggtca attatttaac caacaaatta
    12841 tttggacgct ctaaagagac tctctttgag gaaacgaatg ggcaattgaa tctttttagt
    12901 gatgaagaaa tttctgtaag tgttccagaa gcagctgcca ccattattcc agtaaaagga
    12961 catcaacgag ttgtaggaac gaaagcagac aaaatcaaac acttacctat aacagagaaa
    13021 gaacatctcc ttcctttgga agaacaattt tgtgaacatt gcggttcaca gatgaaagat
    13081 atcggacgga caaaagtacg agaagaaatt cgtttccatc aggctatgtt ggattgtctc
    13141 acccattatc aacataccta ttgttgtaaa agctgcgaaa aagaaggact ttcttctttt
    13201 aaaaaagcga tcgttcctaa acctttgatt tctaatagct taggttccaa tagtttagtg
    13261 gcggaaacga tccggatgaa atttggtcag aaagttcctg cctatcggca agagaactat
    13321 tggaaacaaa cacatgggtt ggatatttcg agagataata tcaccaattg gcatattaaa
    13381 gcagtgcaaa acgcgttaga ccctttagga gaacgattaa gagtctatct taatcaagaa
    13441 gagattctac acggggatga aacaagttat cgtgtgattg aaagtgccaa aacagatacc
    13501 tattactggc agttttgtac aggaaaagac agtcagcatc ccatcgttta ttatcaccac
    13561 gacgaaagtc gtgctggaga tgttccgaaa acttttctca aggagtttac agggtattta
    13621 cactgtgacg gctatagcgg gtataatgcc gtggaaagtg tacgacttgt ctattgtttc
    13681 gcgcatgttc ggcggaaatt tttcgaagca attccaaaag gaaagaaaaa cacggatatt
    13741 cccgcagctc aagcagtcaa acaattggat aaatggtttg tcctagaaaa gaaatggaaa
    13801 gacttttctc ctgagaagcg attaagctgt cggcaacaag aactgcgtcc attatttatt
    13861 gctttttatg agtggatggc aacgatagat cctgttgcaa aatccaagtt ggatgcagcc
    13921 gttcaatatg cgtgtaaact acgaagtggt tttgaaccta tttttgaaga tggtcgttta
    13981 gaactcacaa ataatcgtgc ggagcgaaat atcaaagagc tggtgatagg acggaaaaat
    14041 tggttgcatt ccacaagtct tgaaggagca cgaacttcag gaatcattct gagtgtatat
    14101 aaaacagcag aacttaacgg attgaatcct gtaaagtatc tagagtttct atttgataag
    14161 ataccaaatc ttcccgtact ttcagcagaa actttagatc aactgttgcc ttggaacaag
    14221 gatgtacaac aacatttttc tcgtaactaa aaacgccaaa cattcccgat tctattctat
    14281 aggtcgggtt atttgacgta caggtactgt tctatggggc gcttacctcc tagattgtag
    14341 taataatgga aagggataga agtaaaacga gttgaatgaa ttttttgagg gggagaatat
    14401 tatgggcgac tcagaggaaa cccacattca acatacgttt gacagttttt gtaagaaagt
    14461 agtctgcaat gaggcgttaa atattcaaaa gaagtacgct agatttcgcc aacggcaaat
    14521 atcaatggaa atattggaac aaaaagggtt aggtacagag ttctattatt tggaaccaaa
    14581 tttgaatagt agtggtactt ttattgtcct tggtatgaaa atctttattt ccaatgagga
    14641 gttggcaaat gcgattgcag cattacctag tcaaagacaa gaaatcattt ttatgtcctt
    14701 cttcatcggt cttaatgatc gtgaaattgg cgatattatt gggaaaagtc tcggcagtgt
    14761 ttggtaccaa cgacaaatgg ctttggaaga gctaggaaag tatttgggtg tcaaatatga
    14821 agcaaaataa acgatatata ccattcatac cactaacgac aatcaaagcc gcagcttctg
    14881 gtgattcaat tgccatgtcc atcgttctgt caaaatatca aaattatatt tcacggttag
    14941 ccatgaaacc agtttatgaa gaagaaaaag gaatggttat gtatgtagat gaatatatgc
    15001 gtagacaact ggaaacaaaa ttgattgaga agatacttga atttgataca actcgttaaa
    15061 gtattttttc cctttccttt tcattctatg gaagtttttt atcgggtcaa aataaaggat
    15121 ttccataggc tgaaaatagc ctagcgctct ttgacaaatg aatagcaaat ctatcagtac
    15181 atgtagattt tgattctcta gttacgccat gacaaggtta gcttttaagc gacaaatttt
    15241 aatatgagaa tcgcgactac gataatgata cttccgtaac caaatcggcc cgccattact
    15301 ggggggaggt gagattccta tgtgctttgg caaaacagct gatagatcag aaaaactact
    15361 atttccatat ttcgattttg gttggcatat ttttcatgcc aaattctgtg attttaatag
    15421 catcggcaag atttaatttt tcttcagctc tctctccact tttaagtttt tttactgtac
    15481 tcaagttgat tcctgttccg aatgctattg cattagccgt agatttttca agaatttctt
    15541 taattatttt tatgtctgca attccttgtt tcatattaaa aattcctctt ttctagtccg
    15601 ttttgatgga gtactggagt gtttttttac aattttaagg ggtaccgtgg tggtcgtttt
    15661 gattttgaga gagaagggtg atggagttgg aagttaaaac aaatgttaat tctctggaaa
    15721 tagagttcta ttcaatttta caaaaaaaac tggaagatta cacggaaaaa ttgcttgcag
    15781 tcagtcattt acctctggta ttaacaaata ctgatttaaa gcgactattt cagataagtg
    15841 atagtacact gaaccgttta gtaaaacttg gtgagtttcc tccctgttgg tacgggattc
    15901 gaggacacta ttttcgtgat gatgttttag aatggatgag aaagagtgat tcgaatgact
    15961 atcgagaaaa attgcgacta cttcgatctt tataagttac attttctttc taagatgcgg
    16021 tacaatgatg acatagatag aaagatttcc aaagggtttc tgcttgttgg gttccttcaa
    16081 aataggagga atcaataaat ggaaaaaaca gatcgattca agaagcaaat cagttctact
    16141 ggtaggatca catggtactt tcaagcatat cgaacaacaa gacgtggctt taattcaaaa
    16201 cgtgaggcgc aactcgctta tctggaaatg gagaaacaac aaaggcttaa gaaaaaaatt
    16261 gttgcaagca atgacaagtt tagtgttgta ggtgagcaat ggttccaata ttaccgttcg
    16321 ttaaatgaac aaaaggaaat aacctatgat aagcgagagg aacaattaaa aattattgtt
    16381 cgatggattg gtgataaaaa gttgtctgat ttgacaccag agtgccttca agaattgctt
    16441 tttaccttaa aggaatgtgg cgttaatgga acaaatgttg gctatgccaa aaatagttta
    16501 caatcactta ttcaagttct aaatatgatt tttaaatatt gtcaaagaaa gaatttgata
    16561 agtgaaaatc caatgaagtc tgtgagaatg ccaaaatatc agttaacggt aactgattta
    16621 aaagaagcca taaataatat tgaggacaag tttcttactg tcgatgaatt gagaactttt
    16681 ttgaattatg gcattgttca tgaggaatta ccgatgtctg tacttttcca tgttctattt
    16741 tacactggct gccgagtcag cgaggcgctt gctctacagc ctggagatat agatttcaaa
    16801 agaaatgaaa ttctgttcta taagcaaacg gcagttaaag gtaagagtaa ggattttcga
    16861 attgaaacta ctaagacagt tagttctgct agacgagttc tagtaactcc tctagttatg
    16921 gaaaagctac aacagttgat taaggctttg ggtgagatga ggcaacatta caggtttatt
    16981 atagaagaaa catatttgtt tgtgtatctt gattctagta aacgtggtgt tccttatcgt
    17041 cgagagtatg taaatgatca cgtcaaacgg tgcgtgaatc gctcaggaat taataagcca
    17101 tttcatactc atttagctcg tcatacaatg gcaagcttag tagcggaaca ctgtagctgg
    17161 gaagtcttga aagatcgctt aggccatact gaccaaacaa cttcgaaaat ttatcgccac
    17221 ttaacgtcaa atgaaaaggt aaaacctctg attgctttta gttctttaga gagttaaaaa
    17281 aacggtgaaa gcactggtat tacaggattc ttgaaaaacg attttttaac aaattagtca
    17341 atatgtagtc agaggtaata aaaatccttt tatatcaata ctttaacgac ttttcacaag
    17401 tatcaaagaa atccgtcgtt aatcattgtt atcagactga gaaagaaccc ttggtattca
    17461 agggttcttt tttcttagca tgtaatttga acaccctgat ccagtttttt ctttaattat
    17521 tggtagggga gaataatgga ttagaataaa tctacctata agtagtagta aaaaaaagag
    17581 cactatgata atatctttct taagagtaac cgctttaata aaaaatccag aatattctaa
    17641 cataaatgga gtattcttag aatcaaaagg agaaagaaga tggtttatac ttatacggag
    17701 caagaaaaga tgaaagtttt agacacattc atgaatgatg gaaaagtaaa acaagtacca
    17761 agttcaacaa aaaagaaata catcctattg aaagaagtct tgaaacgctt tgaccatgga
    17821 gtgacttata cagaaacaga ggttaattcg atactgctga acgtattttc tagtggcgat
    17881 tatgtcgaac aaagacgtta tctaatcaca tttggctttt tcaaaagaag ttcagacggt
    17941 cgggcatatc agatgatggg aattgaaaat tagaagtttt aatttatact cattgaaaaa
    18001 agttcaagta tgcgatattg gttctacacc gcatacttga actttttttg tcatctgtat
    18061 ctataataca aagccaagaa cgttattcaa aatatggata gctatgataa aagctaatga
    18121 gcgattcgca gttaaataag caatcgtcag taaaatcgtt ccaggagcgt acatgaaaaa
    18181 atcaattgga tgcgttacat gaataaagat aattctcttt tatttgtacg aataaatacc
    18241 aaaacgtacc tcctaaaatt agattttgga cagatgccct ttaaaaagat aacaagattt
    18301 cgttcttgaa acaatatata tgcttttatt ccaatgaggt tgtgaaaaca gtattttgtc
    18361 ctgagcgtta gaaaattgaa aatggggaat agcaactcgt actttaccat tttttaagtg
    18421 aatacaagat cagctaattg aacaccgttt attcgtgatg agggacacaa cactgcagtc
    18481 gacacatttc tcttttatgt tcaaagataa caaatattta aaacaaagaa aaatagtaga
    18541 taatcccgtc ctaactcttt tattcttccc tcatagcttc caaatttaag caatact
//
LOCUS       DACCYH010000056        18430 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.057, whole genome shotgun sequence.
ACCESSION   DACCYH010000056 DACCYH010000000
VERSION     DACCYH010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.057"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(175..2259)
                     /gene="cadA"
                     /locus_tag="IVY85_001850"
     CDS             complement(175..2259)
                     /gene="cadA"
                     /locus_tag="IVY85_001850"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8044865.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            complement(2401..2580)
                     /locus_tag="IVY85_001851"
                     /pseudo
     CDS             complement(2401..2580)
                     /locus_tag="IVY85_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(2784..3143)
                     /locus_tag="IVY85_001852"
     CDS             complement(2784..3143)
                     /locus_tag="IVY85_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP8044866.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(3173..3514)
                     /locus_tag="IVY85_001853"
     CDS             complement(3173..3514)
                     /locus_tag="IVY85_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044867.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(3511..4185)
                     /locus_tag="IVY85_001854"
     CDS             complement(3511..4185)
                     /locus_tag="IVY85_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8044868.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(5361..6659)
                     /gene="asnS"
                     /locus_tag="IVY85_001855"
     CDS             complement(5361..6659)
                     /gene="asnS"
                     /locus_tag="IVY85_001855"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP8044869.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(6699..7889)
                     /locus_tag="IVY85_001856"
     CDS             complement(6699..7889)
                     /locus_tag="IVY85_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8044870.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(7910..8437)
                     /locus_tag="IVY85_001857"
     CDS             complement(7910..8437)
                     /locus_tag="IVY85_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8044871.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(8484..11273)
                     /locus_tag="IVY85_001858"
     CDS             complement(8484..11273)
                     /locus_tag="IVY85_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8044872.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(11420..11620)
                     /locus_tag="IVY85_001859"
     CDS             complement(11420..11620)
                     /locus_tag="IVY85_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8044873.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(12073..12393)
                     /locus_tag="IVY85_001860"
     CDS             complement(12073..12393)
                     /locus_tag="IVY85_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044874.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(12896..14014)
                     /locus_tag="IVY85_001861"
     CDS             complement(12896..14014)
                     /locus_tag="IVY85_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044875.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            14485..15792
                     /locus_tag="IVY85_001862"
     CDS             14485..15792
                     /locus_tag="IVY85_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP8044876.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            15913..17112
                     /locus_tag="IVY85_001863"
     CDS             15913..17112
                     /locus_tag="IVY85_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP8044877.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(17139..18407)
                     /locus_tag="IVY85_001864"
     CDS             complement(17139..18407)
                     /locus_tag="IVY85_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP8044878.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVRKGILFLPIVKRMKEKNKV"
BASE COUNT         5285 a         3943 c         3224 g         5978 t
ORIGIN      
        1 tttttcatat tttttccatc aaaaaaataa gttattttct tttatttcat tgaccttatt
       61 gaatttcaag ctattttgaa ttttctgcaa aattaaaaac attagttatt ttataatcct
      121 atctattttc tttacatttt tcattaagaa aaggatataa aaaaaagagg acgttcattc
      181 gtcctctttt tttacatgca agcttcgcat ggcattcaaa acagctaata ctgtcacccc
      241 aacatctgca aatactgcat ctcccattga tgcaaaacca aaagcaccta aggcaagtac
      301 caaaattttt atcccaatag caaagacaat gttttgtttg acgattttca atgttttctt
      361 tgctaaatgg attgcttctg caatcttcga tggttgatca ttcatgatga ctacatctgc
      421 tgcttcaatt gcagcatcgc ttcccaatcc acccattgcg attcctacat ctgctcttgc
      481 tagcaccggt gcatcgttga ttccatctcc gacaaatgcg gtctttttat tgtttctttg
      541 taagatttcc tctaaccgct gcactttatc ttcaggaagc aattcgctat atatcttgtc
      601 cacgcctact tgcttcccga tatgatcagc aatttttttc gagtcgcccg tcaacatgac
      661 tgtgttcttc acaccagcct gttttaattg aaccaatgcg tctacggcat cttctttcaa
      721 ggtatctgca ataacaagat aaccactgta actttgatcg atcgctagat aaagcaatgt
      781 accgatttct tgacttaaag gaaaagagat ttcaaatctt tccatcaatt tgtgattacc
      841 tgctaatact tgtttgccat caacttcagc taagatccct tcacctgcaa attcctggat
      901 attagttgtt ggtaaaagcg gacctgcgta gccttctaaa acagattgag caatcggatg
      961 tgtagaaaac tgttctgcac tcgcaatata ttgcatgaat ttctttggat cgatttttgt
     1021 atcgatcgtc tggatcgaaa agtcaccttt cgttaatgtg ccggttttat cgaataccac
     1081 tgtctcggtt tgcgcaagca actccaagta atttgaacct tttactaata caccgatttt
     1141 acttgcgcct ccgataccgc caaagaaact aagtggcacg gaaatcacta atgcacatgg
     1201 acatgagatc actaaaaatg tcaatgctcg atagatccat tcagaaaacg ccgctccaga
     1261 aacaacaagt ggaggtaaaa cagctaataa aacggccaat cctacaacga ctggtgtgta
     1321 atagcgcgcg aatttagtaa tgaaattttc agctggtgct tttttactgc tggcattttc
     1381 tactaattct aataatttgc tgatagtaga attttcataa gatttttcta cttgaactaa
     1441 aaatggttta tttttattga taaatccgct taatactgtt tcaccaacat atacagattt
     1501 agggactgat tctcctgtaa gtgctgacgt atcgactaat gattctcctt cgatgatcgt
     1561 tccatctaac ggtacacgtt caccaggctt cacaagtaca aactgaccga tctgcacgtc
     1621 ttcagggttc acggtcatca atccttccgt cgtctgcacg tgagctgttt ctggacggat
     1681 cgccatcaat tcgcgaatcg attttcttga atgactgact gccaatcctt ggaaatactc
     1741 gccgatttga taaaatagca tcactgctac ggcttctgga tattcaccga tcaacatagc
     1801 gccaatcgta gcaacagaca taagaaaatt ctcatcaaag atttttcctt tgacgatatt
     1861 catcactgcc ttcttcacta catcatatcc tatcagcaga taaatcaata aataccctat
     1921 caagcgaatt ggttcattaa aatcaaataa tagcaataaa gcgatacctg caagggaaaa
     1981 taaaatccgg aacagcgtat ttctctcttt ttcatttcca tgttcagata ctttttcctt
     2041 atctatttct gttactttga catctggttc taattccttc actacttttt ttgtttcttg
     2101 ttcaatcgta gccaaatgat cttcctctgc gtcaatcgtt agttttccag aagtgaagtt
     2161 taccgttgct tccttgactc cattgatctt ctgaactcct ttttcgattt tcattgcaca
     2221 gttcgcacaa tctaaacctt ctaatctgta acttttcatt ttaatgacct cctcgcctca
     2281 acatatgaac atctcttcat aactaattat aaatgaatgg ttattcatat gtcaaggtat
     2341 ccgaaagaaa aaatgaaaaa catctgcatc ggaaaatttc ttccatacag atgttttggc
     2401 ttattctttc tttttctctt cgatcattgc ccctgctctt atccaacccg cacttgcctt
     2461 cttgatcttg acaggatagc aactaagttt gaagcctgtt gctggcaatt tgtctaaatt
     2521 cattaatttt tcgataggac aatatctttt ttcaatccct gcaaaatgtc cttcccagat
     2581 attcgaagct ccactacgct ttcaaaaata tagaaagagt tctttaatga aaattatttc
     2641 aactttccat aaacagtaat cccatataaa taaaagacgt aaaagtcaca tgggagaact
     2701 tatacaggaa aacacataga gtcaggtgaa atacatgtat cccacctgac tctatgcgtt
     2761 ttctaacctt tattattctc tttttataca atttctgcac cgaatggcat caacgccaat
     2821 cgagcaagtt tgaaagattg cttggcaaaa ggtatcccta tgatcgtgat acatagaagg
     2881 atgccgctga cgacatgtgc gagagcaatt ggtaggcctg aaacgatcag ccagatgata
     2941 ttaacgatca atgacatgcc agactggtga taaattactt ctttgccaaa aggcgcaaaa
     3001 gaaagggagg cgatcttaaa acattgcata ccgattggga tacctatgat cgtgatacac
     3061 caaagtatcc ccgctgctaa ccagctaaga ccaccgatta gtccgccgaa tataaaccag
     3121 atgacatttc ctaaacaact cattgtattt ctccttctat tactgttttt gattaaaact
     3181 gggcaaaaat tgattttatt cttctttttt tagcttccag catcaagtat tctgcaaatt
     3241 ttttttggat ttcttccgta ttttcttttg ttactaatgt gtgttggatt agttttcctg
     3301 atggtcgctg atccacccgt tcttctgact gttgcacgtc cgcttgcgca aatccgataa
     3361 tccgtttttc ttttgttaca gcaacgagca atagtttatt cttatcattt aggtcagctg
     3421 ccgtttttgc ttttacgaat gattctggat gatcagtaat cagctgattg atttgaggta
     3481 aatcttcttt tactgcccac cgtatttcca tcatttctca cttttctttc gctcattttt
     3541 atctccacta aaaaaatcga ctgcggcaat tgcatctatc cagctttgtg caatgatctc
     3601 atgtccgcca atcgttgggt ggaccccatc ttctcttgta tatttcgaaa aaccataagc
     3661 aatccctaaa gcattgaata tgccatctaa aggaaccagc aatgcgccat attccttagc
     3721 aagtttacga acaatttgga ttttagggtc taagtcttta cgccagccat gacggtcgtc
     3781 ggttatagga agtacaaacg gctccatcaa gatgatgttg cagatttccg ctgtttgaag
     3841 ttcatttagt aattttcgat aaacggattc aaattgctgc gcagatttct cagttccgaa
     3901 tatagcctga tcgctagtat tatgccaggt atcattgatt ccgatcaaaa tcgatacata
     3961 gtcaggttgt aaagctaaac aatcttcttg ccagcgtact tgcagatctg caattttgtc
     4021 tcctcctatc ccacggttca tgcattcaaa tttcatcgtt ggatattggg agattagttt
     4081 tccggcaatc aaaagtggat aacctttacc tagatcagtt gcttctgctc ggtctcttcc
     4141 tgcgtctgta atgctgtcac cgataaataa tatacgtttt ttcatttgtc aaaaactcct
     4201 ttttgaataa aacgatgatt ttcttcctta tattatccca taagccctat caagacgaaa
     4261 gagaaaatat atgtataaaa aatgacacca gcctagtccc ttcaatcaat tgaatgaacg
     4321 ctcatcaagt gtctagtttt ttatactttt tttcttaacg tgccggattc cccttgattc
     4381 cttcgttgtt tggtccagag aaatcgatca cacctaagct tccgtcaatc agaccactga
     4441 tgacgccatc agaacatata tttataggct catctaagac ttccagtaaa tgttctttta
     4501 ctaatatctc ttctagattg cttgggatag attggtcata actttatctg gtccgatgat
     4561 ttcaaggatg aattaaactt acatatggat cattgcatgc tgaaaatacg gacctactcc
     4621 atcttcgaat gttactatta gtttgttttc aggtactaga tgctgcttta tttcaacagc
     4681 agaagcatca tcaaatcaga tttccatttg aataaccttc tttgtatatg tttgattgat
     4741 tgcgatttcg ttatagaacc ttgtgaatca aatagcaatt tcttttgctt tcttctcttg
     4801 tctgctggaa acttccttta tcccgtattt taaaaaaaga actgcgagag ttcgcagttc
     4861 ttttttttaa ataaacgttg ctctccggcc aactcctcgg tcacaagtca caatagtcat
     4921 caaacatgaa aaactgtttt ttgaaattac tccttgtgac tcggagctga acggcaggcg
     4981 cacaacctct tgatataaac ggtgttcaat cagctgaccc tcgatattag ctaaaaaaac
     5041 ggtaaaatat gagaagctat ttttccgttt tgtttctcta atactcaggt caaggcactg
     5101 atttcacaac ctcttcctta aacggtgttc aaaagccagt ttctcggtat taagccaaat
     5161 ttatgcaaaa atgtggaaag ccatttccac aaaatttttc ttaatactcg aaactaaaca
     5221 gctttttcac aacctctcca ctaaacggta ttcaaaagct tacatctgcg gtaataagtc
     5281 ccgccaattc aaaaacatga acagctgttt ttgcttggct gttctaatta cttgatgcag
     5341 aacagctttt tcatcacctc ttatggataa attcggttca acaaacgtgg gaatggaata
     5401 gcttcacgta catggtcgat accagcaatc caagtgactg ttcgttcaag gcctagacca
     5461 aaaccagagt gcggaacaga accgtattta cgtagatcaa gataccatga atattctttt
     5521 tcatccaaac cgtgattacg gatttgttct agcaagaaat catagtctgt tgcccgttcg
     5581 cttccgccaa taatttcacc gtaaccttct ggtgcgatca tatctgcaca aattaccacg
     5641 tcttctcttg ttggatgtgg tttcatgtag aatggcttga tggcttttgg atagtttagg
     5701 ataaagacag gctgttcgaa tgaattcgca atgaacgttt catgaggtga accaaagtct
     5761 tcgccccatt cgatgtcatc aaatccattt ttcttcagta attcgattgc atcatcgtaa
     5821 gtaatacgag ggaatggcaa ttttgtataa gtttttaaga cttctttgtc tcgtcctagt
     5881 acatctaatg catattcgca atgatctaaa acattttgta ctaagaatgc tacatattgt
     5941 tcctgtactt ctaaactttc ttcttgatga gtgaaagcca tttctggttc gatcatccag
     6001 aattcgatca aatgacgtcg tgtttttgat ttttcagcac ggaaagtcgg accgaatgaa
     6061 aagacttttc cgaaagccat agcagcagct tccatgtata attggccact ttgagataaa
     6121 taagcttttt gatcaaagta atttgtttcg aatagatccg tagtgccttc tggtgcagaa
     6181 ccagtcaaga tcggcggatc gattttgaca aaatgatgtt gattaaagaa ttcatacgtt
     6241 gcacgaatga tttcatttct gatctgcata atcgcatgtt gtcttgaaga acgcagccat
     6301 aaatgacgat gatccatcaa gaaatctgtt ccgtgttctt ttggtgtgat cggataatcg
     6361 tggctctcgc ttaccacttc cacatttgta attccgattt cataaccaaa ttttgaacga
     6421 ctgtcttcac ggatttctcc agttactaac acggatgttt cttggtgtag atttttagct
     6481 gtttggaaaa cttcttccga cacttcgctt tttacaacta caccttggaa aaaggctgtt
     6541 ccgtctctta attgtaaaaa tgcgattttt ccgcttgatc gcttatttgc gacccaagcg
     6601 ccgattgtta ctgtttctcc cacatggttt ttcgaatcga tgatttgaat ttgttccacg
     6661 tatgactctc cttttatatt ccttgaattt cttcaggatt aattttgact tttactctca
     6721 ataaattgct tgattctgcg taccgcttct tctaacgtag cgcgatcagt ggcataactc
     6781 atgcggacat tttctggtgc cccaaagcct tcgccagtca caagcgctac ccctgtttct
     6841 tctaaaagat cttctaccca ttgtgtgaca ttttcatact tacacatgcg tagcgtttct
     6901 tttacattag gaaaaagata gaaagctcct tgcggctttt caagagaaac acccggcagt
     6961 tctgccacaa gcggatataa cgtattcagg cgttcttcaa aggcgttccg catttcttct
     7021 actgtatctt gctcaccttt caatgcttca acagctgcat attgactgac tgctgtcgga
     7081 ttgctagttg attgggaagc aacagcgatc attccattga tgatcacagg atttcccacc
     7141 gcataaccaa tgcgccagcc tgtcatcgca tatgttttag acacgccgtt gatgatgatg
     7201 gtctgatttt tgattgcttc tgaaatcgtt gcaattggag taaactcatt gccattataa
     7261 actaaacggc cataaatatc atcagcaact atcaaaatat cattttttac tgcccattca
     7321 ccgattgcct gcagttcatt cttgctataa atcattccag tcggattcga tggagaattg
     7381 atgattacgg cttttgtttt ttctgaacgt gcttgttcta gctgttcaac agtaactttc
     7441 cagctattgg attcttcccc tttcacaaag acaggaagtc cttctgccag ttttacttgc
     7501 tcaccgtaac tgacccagta aggcacagga atgatgactt catcttgcgg gtcaaggatc
     7561 gtttggaaaa gggtatacaa agcaaatttc gctccgtctg ttacgactgt ttgtgaaggt
     7621 tcatagacaa gtccatagtt tttcttcaga taatcaacga ttgcctgtcg aagctctggg
     7681 attcctgctg ttggtgtata atagctagct ttcccattgc ggattgcttc gattgctgct
     7741 tcttggatgt tttctggtgt cgcaaaatcc ggttctccca cagtcaaact cagcacatct
     7801 ctgccttttg cttttaatgc cttggccttt gccgcagctg ccaaagtgac agacggttct
     7861 aatcgttttg ttctttttga taactccatc ccattcattc ctttaccttt taaattccct
     7921 tgattgtctt gacttcatct ccagattcaa agccaatcag gtaataattc agtttcccat
     7981 ttgtttcttt tcctactact tcccacacag gctgatcatc atacatccct aaagcagctt
     8041 tttcgatttg gatttcagga tgggccgcag caattgtctg ttttgcttcg ttttcagtca
     8101 gtccctttgt ttgatccaat acttttactt tttcaccaga tttaggaatg ataacggcga
     8161 tttcttttcc tttatcattt gtacctgtca gtgaaaagta tgtcttcttt cgagtaaacc
     8221 aataaaaatg atcggtttcc tcaagatttg catatttctt tgcgatttct actgcttctt
     8281 tcttcgcctg caccgccgga cgactggaac gcaagtaaaa aatcgagctg atcacgatga
     8341 ccgctaataa aaatacgatc aagccaagta acacgcgatt aatcgttttt tcctttgtct
     8401 gttttttctt caagggtcgt cgctccttta tcttcattcc ttttcattat agcagatttt
     8461 aattatgaaa aaagacagct cctctaactt ccttcttcat ttttctttaa gaattcatgg
     8521 atatcctgat agatttcctc catagctagc tctttgatcg gtactttttt cggtaatgca
     8581 tggatgatcc gtgcgccata ttttgcagtg actagtcttc gatccaaaat aagcaatacg
     8641 ccacgatctg tctccgtcct tatcaagcga cctaatgctt ggcgtaactt caaagctgct
     8701 ttaggtacgg attcctggta aaaaggattg atcccctctg attctaaata agcgttccta
     8761 gctttgacca tcgggcgaag cggattatcg aagggtaaac gtgtgactac aacgatctgc
     8821 aaagcatctc caggcaaatc tacaccttcc caaaagctgt cagcgccaaa caatacgctt
     8881 ttttctgaca aaatgaagcg tttcaaaagc ttctcccggc ttccaccaaa cccttgagct
     8941 agtatttctc gtcctttttc tagcatagac agatgcattt tctgatagac cttctgcaaa
     9001 acatcatgag aagtgaataa cacaagtatt ggccgatctt cttgttcgat cagacctgta
     9061 agtgctgtct ctaaataatc gatatatgct tgattgctga cttgatggac ggcaggtgcc
     9121 tcttttggca catagatcct tgcctgtttc tcataatcat aaggagaggc aatcacttta
     9181 agaggtgctg cttcaatgcc aagcctctta gagagatagt gtcgattagg gccgatcttc
     9241 agcgttcctc caatataaag aatacgatca tatcgaggat accaaacagt cgacggcaac
     9301 atagccgctt gaaaatcatg gagatgaaaa atcatctgct gcgctgtgtt cccaggaacg
     9361 aaccaatgga tcaaatgatc tcgccagtct tccagccatt gatccatcag caagtattgc
     9421 ttgtctaatt gatcaaatag gctatataaa cttgctaaat cgatccgcat ctggtttgtc
     9481 cagctttctt ggataatctc aaaagtcaga cgcatttgtg actgtagctg ctggatctcc
     9541 tgataaaaaa gctgcaggtg atcaatggct gtcccagatt ccagtggcag ctgatccaac
     9601 atctcttttg tcatcatgac ttcttctgga tactgtccgt taggttgctt gccgctcact
     9661 aaactcatta atccttctgc catacaaatc tgtgcttcgc atagcgcaga aagttcttct
     9721 cgataaatcg ataaccagcg taaggcttca tgttctttcg gtaaatagct ttgtattcgg
     9781 tcaaacagct gatttggttc ttctaattgc tggatttgtt tatggaaata gttaatactt
     9841 aatcgtttgt catttacttt ttcagcgatt tccggcaaat gatgtacttc atcgatcaac
     9901 aaaaatggac tagctggaag caatggttct tttcgctgtg tttcttgtac taaaaacgca
     9961 tgattgacaa tcaaaaaatt actttgcctg attttctttt gcaagaaacg aacaaagtct
    10021 tgttcgtaaa atgactgatt tcttgctaaa aatccaatac ctctgtgcgc gatatctgca
    10081 aagaatgaat ggttcaaatt tgttaaatgt aattcatcca aatcaccagt cttcgtttgc
    10141 gtcagccaaa ctaagaccgc catttgatac aaagcatatt gtttttgttc tggcggttcc
    10201 acaagtgtgc ctttaaaacg ctgcaaatca atatagtggc gatagctttt gataattaca
    10261 ggttgtatgg gctgatctag caattgattc agcaatggaa tgtctttttc tatgatctgc
    10321 tgctgcaaaa ctagcgaaac ggtactgatg acagcgggtt tttcaggtgt agccaaataa
    10381 ctcaatggca gcagataacc gattgtttta cccattcctg tagctgcttc gatcatcaaa
    10441 tcttttgatt catcttttgt gaaatggtcg tagaccgcat tcatcagtct gttctgttct
    10501 tttcgataca tcagtgtttt accaaacatt ttttcttttg cttgtttttt tcttggatat
    10561 gtccgctcgc cataatggac ggatgtgaat agttccactt cttttttctg caaagctagc
    10621 ccatcgacga tttctaatga gggatcaagt ggctctggtt tttctttcat ttcttcaatc
    10681 acatgataaa tgaaacgact cgtatccata cctgtgtgtc tacttaatcg cgcaatctgc
    10741 tctaacgtga tgattggcaa ttttctcatc cgttcttcga tcagcaaaag aagttgacct
    10801 gtcacttcgg catcactatc tgcctgatga ggattctcat gaataaatcc aaggctttct
    10861 gacaaatcag ctaagcgaaa gcttggttcc gtcggcaaga aaatttgtgc aagttctact
    10921 gtatcgatgc ctggtatctt caaactagga agtccgcatc gcatgagttc atggttcaaa
    10981 aaattataat caaaataaat attatgcgcg acaaatatgg tgtctgccaa aagattgtaa
    11041 atcgtttggg cgacatcttc gaaataaggc gctttttgga cgcgttgatt cgtgatccct
    11101 gttaaatgct ggatttgttt tgaaatcttc cgccctggat gaatatcgat cgaaaaacgt
    11161 gaagtgatcc gacctgattc aatcatgacg cagccaaatt ggatgatccg atcttctttt
    11221 ggattcgtcc cagtcgtttc gatatcaacg actgcataag ttcttggttt caaaatatct
    11281 tcctcttctc ctgcctctaa tgttcttttg aaattatact acaaagtgca gaaaaatact
    11341 ctcatcgctt tttaaagaaa atagacaaaa acgcccagac acgcaaatgt ctgggcgttt
    11401 attttttagt ataggttaat taagctttag taacgtttgt tgcttgaggt ccgcgttggc
    11461 cttcttcaac atcgaaagtt actgcttggc cttcttctaa agttttgaat ccgtcgcctt
    11521 ggatagctga gaaatgaacg aatacatcgt ttccgttttc tgcagtgatg aatccaaatc
    11581 ctttttctga gttaaaccat tttactgtac ctgtttccat aataaaaatc ctccttgtgt
    11641 atcaacacat attttgcaat tacttgaatg gtgtatatct ggaagatgtt tatttgaaac
    11701 aagctaccac agattaccga acaaaaacta ctcctatagt atatcatacc gtttacaaaa
    11761 aagctagagt ttttcgactt tttttattaa aaaaagtttt ctcagcgatt acataaagtg
    11821 aaaaaccgaa ctttttttta aaataaacga catattgttc agttggtgtt tttttgctct
    11881 gatgttcttt tttaaactaa aaaaattatt ctttctgact acttatcggt tctgtttcaa
    11941 cggtcgaggc tggggaagat tctttagtag gtctgatgtt ctcatggtat ttctgatagc
    12001 ctatgacagt taccagaaga acaaggcaaa ccaatacgac caacgagatt agtttttctt
    12061 ttttgctcat atctatacct cataaataac aggatgatgt ccatcatatt tctgagtatg
    12121 agtaaaagca aaagctgcag ctgtagaatg taagaaatgc cagcaacttt cataatcaca
    12181 ggtcggacag taccaaaagt aaccaaatct tggctgcaaa gaccgttctt ctgtttcagg
    12241 aatcgcagaa tcaagatctg gtgtctgtac ataaacattt ttgtacagac gtgtgcctgt
    12301 aataggaaac cgctgtgttt cttcagcaaa aactggctga cttgttaaac ttagggatag
    12361 agacagcaaa caccataaca gtcttttttt cataaagacc tccttatcga taagtttttc
    12421 ccaattaaaa tcatatctca agaacgcccg ttattcacat tttgttcata aatgatgttt
    12481 tttgtatcat aatcgtcaaa aagaggtcgt gaccacgtca caacctcttt tttaaacggt
    12541 gttccagaag tagcttcttc ggaaataagc cgaaatttca caaaaatttg agaaataatt
    12601 tttgtcaatc tcttcttatt tcttgaagct gaccacttct gtcacaacct cttccttaaa
    12661 cggtgttcaa aagcttgcat ctgcggtaat aagcccacca aacaaaaaat atgaagagct
    12721 atttttgctt gctgttctta ttacttgatg cagaacagct ttttcacaac ctctcataca
    12781 cggtgtttaa tcagctgacc ttcggtatta gctcaaaaaa cggcaaaata tgtgaaacta
    12841 tttttccgtt tgtttctcta atactcaggt caaaacactg atttcacaac ctcttttaat
    12901 aacgaagaat cgctgtcaga ctcttttcat ctggcgcatc tttttgatcc aaaataaaca
    12961 cttttccgcc attaatgatc acatcatctg ctaatgcatt caacacttga cgccgatcat
    13021 attctgtttc aggatcttca ccgaaaccat cagctaagtt tgacgtagcg ataaataaat
    13081 gtgacacttt tccttctttc gcagctggaa cgatatcaac taactgatcg atgaatttct
    13141 tatctaagag tttattgtat tcttttgatt cttgagctgc gagttcactt gacagcttct
    13201 tcgccccttc ttcgatatct ctaaaggata gctgcgcagg agaagcagaa atcgcaacgt
    13261 ctgcacaata atagggattt ttagcaattt ttttgaacat cgtctgattt tctggtagag
    13321 caaataaata aatcggccat tgttctggat tttcgaaaga atccttcaag tacgtatcaa
    13381 ctgcttggta atagttgacc caatcgatct ctacttcttc atcttttgga tttacgccat
    13441 ggaaagttac tccatctcgg gctccgcctc ctccattaga agagaagttc aaaccgccac
    13501 ctgtcagttc agatcccaat gcagtaacga catcaaccgg tgccccttct ggtagagata
    13561 cttcttttac ttgtttgttg ctgacttcat acaatttcat ggagtcgcgg ttcaatccca
    13621 ataaataata agaatagttg aactgcccat ttttgataat agccagtaaa taaggaagat
    13681 cacttacata gtattgatcg tctaccggga tactcaaacg atggatatag gtttggtcag
    13741 cagttataat gaccgctaca ctcgtagttg cactacgcca aaacgaagga tctcctaaca
    13801 aattttcaat tttttcttgg aacggggacc agtcatgttc ggaatatttt ttctcaaaac
    13861 gtttctttgc ttctttcgca aaatttttga atttgatctg atctttttcc acatcttgat
    13921 gggcgacatg cgtattcagc atgatcgtga cgaatggtcc tttagcgcct tctgaagtca
    13981 attgtgacaa taattcttta tccttttttg ccataaacgt acccctccta aaaaaatcat
    14041 tcctttgttt ctatctaaag tctaccacaa cactattttt ccatacaata aaacgtatct
    14101 aaaaaaataa aacggtttct acggttttaa atttagctat ttttttaagt tatcttcata
    14161 atttcctcaa ttatttagcc ccagtcttca actattcttc atgaaatgct gataaactaa
    14221 gaaaaagaat aaaagaggtg gatcttatgc atttgattat gattttcagt ttattaagta
    14281 ttatcagttt atttgctttc agcgcttccc gcttattaga tgcggaaaca cagcctattc
    14341 ctgtaaaagt acgtaaaaac gaccgctaac cttccataac gtgcgttctt atcctaaagt
    14401 tttttcagaa acattgaaac tctctcccct ctctgataaa attttaattt gtagcgaaac
    14461 aaattaatta caaggagatc ggatatgtcc ataaaaaaaa cacgtctagt cctattattg
    14521 gctagtctca ttctttgcct tgcttctttt ctaccagctg ttgctgtgca agctgaagat
    14581 actttcaaag taaatgcaaa agcagccttt gctgtagatg cgcaaagcgg taagattctt
    14641 tatgatcaag atggagaaaa aaccatgggg atcgcttcaa tcacaaaaat catcggtctt
    14701 tacatcgtcc ttgatcaagt aaaagaaggg aaactatcat gggatgataa agtttcaatt
    14761 tcagattatg cagaaaattt gagtgtcacc ccagatttat caaatgttcc tttgcataaa
    14821 gaaaacacct acaccgttaa agaattattc gactctgcaa tcatccagtc agccaacgct
    14881 tcaatggttg ccttggcaga aaaaatctct ggcagcgaag caaaattcac tgaacgaatg
    14941 aaagaacaat tgaaagattg gggtatcaaa gatgcaacga tcgttaatgc atcaggactg
    15001 aataactctt atcttggcga aaaccgacca gaaggtactg gagaaaacga cgaaaatcaa
    15061 atgtctgctc aagatgtggc tatcgttgcc cgtcatttga tcttggattt tccagaaata
    15121 ctggatgtat ctagcactac gacacaaatg tttggcgaaa acacgcaatc accagtagaa
    15181 atggtcaact ggaactggat gctcccagga ttcgtgaatt ataaagaagg cgtggacgga
    15241 ttaaaaactg gtacgactga acttgcagga gcctgtttcg tcggtacgat cactaaaaac
    15301 gatcaacgaa tcatcactgt cgtattgaat gcagacggcc atgcggaaaa cccaagtgcc
    15361 cgtttcaatg aaacagctcg cttaatggat tattgctacg acaattggtc agaaaaagag
    15421 cttggaaaag ccaatgcaag tatccctagt ctaaaaacca ttgatgtaaa agacggaaaa
    15481 gaatcaacag tgcccgttgt gcttaaaagt cctgtaaaag tatgggtacg gaacgatatg
    15541 gatactggaa aattaacaat cacaccaact cttgacaaaa aacaagtcac tgacaatgag
    15601 atccaagcac ctatggataa aggaacaaaa atcggtaccg caacgatcac cttggcagaa
    15661 gataaattag gttatcttga agacaacaac gcgccatcca cagaaatcat cacggataaa
    15721 accgtagaaa aagcaaatat tttcgttatt ggctggcgac gcgtcgcaga atttttcggt
    15781 aacttgtttt aattgaacaa aaagaaaaaa gcatctttcc atgtggaaga tgtttttttg
    15841 ctttttctag tttaaataac agcttttttc atctattttc gttatactgt ataagaaaaa
    15901 ggagttgaat ttatgaatct aaccagagat ctgatttttt tggttctcta tcttattggg
    15961 gtattctcca tcgtttattg gcgaaaacag aaaaaggatc cttccttgtt cttttacagc
    16021 agtacatgga cgtttgcggt aatcttgact tattttgtcg tgttggaaat tttttattcg
    16081 accttttatt ttttgattcc cttagtgttt ttcagtatct ttgccttttc ctatttttca
    16141 gaaaagcgaa ggctgttgaa tggtctttta ttcaatattt tcctcattag ttttggtatc
    16201 tacttgtttg tcttgctata cgaaacacaa aatatttttc ttggcggact gattgccttg
    16261 atcacgatcc cgttactgct tgtctttcta ttcggcattt atggattgat cgtttttctt
    16321 ttttggaacg gtgtcacagt tttacgtaga gagtcgcatt cattagctaa tcttttaacg
    16381 ttgatactag ccatttttct tacattgttt cttgtttttg actttttctt gttaaaatat
    16441 ttaccacaat ggataaatgc tttgtttttc tgcatcccat tgatcttgat ctatctattt
    16501 atcgtattct acaacttttt gactgtctca ttcttgtatc agttcaaccg ccctcgatac
    16561 aaccaagact tcatcgttgt gttaggcgcc gggttgatca atggagaaac agtttctcct
    16621 cttcttgcca agcgaatcaa taaggcgatt gctttttatt gggcacaaag ccgtgcgaca
    16681 ttaaatccgc ctatcctatt aatgtcgggt ggacagggag cagacgaaaa agtaccagaa
    16741 gcaatggcga tgaaacaata tgccatggaa caaggaattc ctgaaagaga tattcttgtc
    16801 gaaaccaatt cgacaaccac tttggagaat atgcgctatt caaaggaaat catggatcag
    16861 caaatgaaag gtccctatcg cgcgatcttc agttccaaca attaccacat tttcagagct
    16921 ggtctctatg cacgacaagc gaaactgaag gcaaatggca ttggggcaaa aacagctttc
    16981 tattacttgc ctaacgcgtt tttaagagaa tacatcgcca tcttgatgat gaacaaaaaa
    17041 agacatatga tcatttgcgg attgcttttc gcaggttccg cattcttatc actgatcact
    17101 ttacttttct aaaaaaacaa ggtgtgacaa aacatatgtc acaccttgtt tttttctttc
    17161 attctcttaa ctattggcaa aaagagtatc ccttttctga ccgctacttg ctgagcagaa
    17221 taaataccgc tgttgcctga acacataaag ctgatcaagc agatcataaa gaagtatact
    17281 gccgcttgtg aaccgaacaa ttcgatcccc atcacaaagc aggcaatcgg tgtatttgtc
    17341 gcaccagaaa atactccgat aaagcccaat cctgcaagaa aaggaacaga aacatggagc
    17401 aaaggcgcta gtgctgcacc aagcgttgca ccaatttcaa acaatggtgt cacttctccc
    17461 ccttgatatc ccgcgcctaa agacaaaact gtgaagaata acttcccaat aaaatcatag
    17521 acatgtgcct gtccattaaa tgattcctgc agtaatggaa gactcaatcc gagataacgt
    17581 tgtgtttgca aaatcaatgc tgcggctaca actacagccc cacctatcgt atttcgccaa
    17641 acaggatgag gaatccattg tgcatatcgc gcttttaaaa agacgatcga acggctgaat
    17701 acccaaccaa tcaaaccaaa agcaatccca gcaacaaaaa ctttgaagaa cagcaacata
    17761 ctccattcag gaatcacacc catttggtaa tgtagatgag aaacaccata gctttctgtc
    17821 ataaaatttg caaacagtgc agcaaagaag ctagggaaga tcgcttctgt tcggactttc
    17881 ccgattgcca acacttctaa tccaaataat gttccagcta aaggtgtgcc aaatacagaa
    17941 ctaaaacccg cactgattcc actgataatc aagatttccc gttctgattt atccaatcgg
    18001 aacaaacgtg ctgtattatc tgctaacgct ccccccattt ggactgctgt tccttcacgg
    18061 ccaacagatc ctccaaataa atgggtagtt atggttccaa acagtgtaag tggaataagt
    18121 cgcaacggga ttttttcttc tccaccattt ccttgatcga tcactaagtt gtttccacga
    18181 ctagaaagac cgccgtgata cgcatagaga taagcaaaaa gcgcaccact aactggtagt
    18241 agataaagga gccaaggatg tgatagtcgg atatctgtca caactgtcag gctatttaag
    18301 aaaaaagcag ataagctccc catgaatata ccgagaatcg ctgtgatcac cagccacttc
    18361 agcatgtaca ctaaaatgcg tactggactc ggtaattgtt tttccataaa aaattcctcc
    18421 tcaataaaaa
//
LOCUS       DACCYH010000057        18051 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.058, whole genome shotgun sequence.
ACCESSION   DACCYH010000057 DACCYH010000000
VERSION     DACCYH010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.058"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            74..364
                     /locus_tag="IVY85_001865"
     CDS             74..364
                     /locus_tag="IVY85_001865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044879.1"
                     /translation="MKLTDATNYMLELSSNLETIDNLIKDLNSSYNMNDQVRVYLEKD
                     DVTLDYWAKSKSFNVSFEKEIIISALKDQRKVLYTELIKTKEFISELLEEKQ"
     gene            361..642
                     /locus_tag="IVY85_001866"
     CDS             361..642
                     /locus_tag="IVY85_001866"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044880.1"
                     /translation="MKTSYKQLDELISLKNRIKHNKTHLKYITECIDEVKKSSGDPYR
                     IKVSYDNHYLTLVSITKEQVINNLEKEKLKLTTAILEIQDEIIEILEEK"
     gene            642..914
                     /locus_tag="IVY85_001867"
     CDS             642..914
                     /locus_tag="IVY85_001867"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044881.1"
                     /translation="MKYSLEQKDEIKSTIFRIRLKRHELEELVDEVNDIAKDPEERYL
                     LSFYYTEEERLFEIPSARLIDYYNTKITNLKSEIISLEKRSQKLVK"
     gene            1036..1302
                     /locus_tag="IVY85_001868"
     CDS             1036..1302
                     /locus_tag="IVY85_001868"
                     /inference="COORDINATES: protein motif:HMM:NF037583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044882.1"
                     /translation="MSRKLMQRNVTSTKVEFSEVIVQDGAPTIVPCEPVVLTGKLSEE
                     KALSAIKRKNPDKNVVVINVSHETALYTMPVDKFIELADKSTQA"
     gene            1347..1718
                     /locus_tag="IVY85_001869"
     CDS             1347..1718
                     /locus_tag="IVY85_001869"
                     /inference="COORDINATES: protein motif:HMM:NF037548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044883.1"
                     /translation="MEIVKSTFDTQTPEGMLQVFNATNGASIPLRNAIGEVLELKDIL
                     VYSDEVSGFGGAEPSQAELVAFFTEDGKTYAGVSAVATKSAKNLIDMMTANPDIKPKI
                     SFVEGKSNGGQKFVNLQVVSL"
     gene            1803..2648
                     /locus_tag="IVY85_001870"
     CDS             1803..2648
                     /locus_tag="IVY85_001870"
                     /inference="COORDINATES: protein motif:HMM:NF017271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044884.1"
                     /translation="MAVRISKNDRAKLDKIYGKSNKARKKYNRLRQKGVEERQLPTVP
                     TSKKSLIDYVKSTNMSRSDFNKLLDELVDFAQPYNENYIFEINKRNVAISRAQIKEAH
                     IKTEQAQKAKEEHYKELNKVQVKKPTENTIVTPTILTELGADLPFQAIPDFNIDSFTS
                     PEGVQSYLENIGKQDEQYFDERDQLYYDNFRQAMFTIFNSDADDIVRLLDSMGLDLFM
                     KTYVSNFLDMNLDYIYDEAEVQQKKEQVYSKIAKVIESETGGEVPSYNPTKNITINSE
                     TGEEL"
     gene            2642..4366
                     /locus_tag="IVY85_001871"
     CDS             2642..4366
                     /locus_tag="IVY85_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015968361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044885.1"
                     /translation="MMIKKYTGDFETTTDINDCRVWSWGVCDIDNVDNITFGLDIDSF
                     FEWCEMQGSADIYFHNEKFDGEFMLSWLFKNGFKWAKETKEERTFSTLISNMGQWYAL
                     EICWNVKCTTTKTGKTKKEKQRTIIYDSLKKYPFPVKEIAEAFNFPIKKGEIDYTKKR
                     PIGYNPTEDEWDYLKNDIQIMAMALKIQFDQGLTRMTRGSDALGDYKDWLKATHGKST
                     FKQWFPILSLGFDKDLRKAYKGGFTWVNKVFQGKEIGDGIVFDVNSLYPSQMYVRPLP
                     YGTPLFYEGEYKPNNDYPLYIQNIKVRFRLKEGYIPTIQVKQSSLFIQNEYLESSVNK
                     LGVDELIDLTLTNVDLELFFEHYDILEIHYTYGYMFKASCDMFKGWIDKWIEVKNTTE
                     GARKANAKGMLNSLYGKFGTNPDITGKVPYMGEDGIVRLTLGEEELRDPVYVPLASFV
                     TAWGRYTTITTAQRCFDNIIYCDTDSIHLTGTEVPEAIEHLVDSKKLGYWKHESTFQR
                     AKFIRQKTYVEEIDGELNVKCAGMPDRIKELVTFDNFEVGFSSYGKLLPKRTQGGVVL
                     VDTMFTIK"
     gene            4379..4549
                     /locus_tag="IVY85_001872"
     CDS             4379..4549
                     /locus_tag="IVY85_001872"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044886.1"
                     /translation="MDLYKAIFIVRDEGTIDGFDTEHYVDISLHDFEEIYAKETREIE
                     AVTLVKTGNFKK"
     gene            4818..5108
                     /locus_tag="IVY85_001873"
     CDS             4818..5108
                     /locus_tag="IVY85_001873"
                     /inference="COORDINATES: protein motif:HMM:NF022859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044887.1"
                     /translation="MEIKEHEAILNGILESVTDGEARSKIVEHLEALREDYGATTEAL
                     TSANSTLEKLKKDNEALVISNSKLFRERASVEPTENNEPETDQNITLDDLGI"
     gene            5122..6450
                     /locus_tag="IVY85_001874"
     CDS             5122..6450
                     /locus_tag="IVY85_001874"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Other names: p8; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head protein"
                     /protein_id="HAP8044888.1"
                     /translation="MADKITEQDVLNATNVETPVQLMTAIYNSSSSLFQANVPMPNAD
                     NIQAVGAGITRLDVVKNEFISTLVDRIGKVVIQYKSWRNPLKMFKKGNMPLGRTIEEI
                     FIDIAQEHKFNPEESVTGVFKQEVPDVKTLFHEINREGYYKQTIQEAWLEKAFTSWDN
                     FNSFVAGVMNALYTGDEVSEFEYTKLLIANYQEKELFKEIEIGEINESNAKEFIRKIK
                     STSNKLEFMNNSYNAQGVRTSTAKADQYVIIDADTDATIDVDVLAAAFNMSKTDFVGH
                     KIVIDEFPKKEGEESSNIVAVIVDSEWFMIYDKLYKTTSLYNPEGLYWNYWLHHHQLY
                     STSQFGNAVAFVKSSTKPVTKVAFASATASVVKGSSKDINITFTPVDATNQQGEVVSS
                     APSLVKATVKETSGKATAVTVEGLEVGQSLVTFTAIGGQQATVLVTVTSD"
     gene            6462..8276
                     /locus_tag="IVY85_001875"
     CDS             6462..8276
                     /locus_tag="IVY85_001875"
                     /inference="COORDINATES: protein motif:HMM:NF028148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044889.1"
                     /translation="MARRYTHVKLLANVPFDNTYAHTRWCKTQQEQESYFNSFPVLNE
                     NTDCSYQRDTQLGGVFRVDKHKDALYACNYLIFKNEETYPSKWQYAFVTDIEYKNDNT
                     SFVTFEIDVLQTYRFDIGIRESFIAKEHPQLYYSNGVPFINTIEESLDYGREYTTTNV
                     TTFHPNDGVNFLVILTSEAMPVGDKEDKSGGSIVGGPSPYSYYLLPLNSSGEVYKPNG
                     AGNANFGEYMAFLTTKEPFLNKIVGMYITSYTGIPFIVDHANKTVRYNAGGSYKITLP
                     TYASDPTGTMKTFAFFCVKEARTFVPKRIDLVGNVYNYFRESFPFNVKESKLFMYPYC
                     LLEITDTKGHVMTIRPEYLTGGKLSVYVKGSLGISNKVMIEPIDYDVTNSSIITNLSD
                     KMLIDNDPNDVGVKSDYAAAFMQGNKNSLIAQEQNIRNTFRHGMGNSAMSTGGAIFSA
                     LASANPFVGLTNIMGAGQQVNNYVAEKENGLNLLAGKVADIENIPDNVTQLGSNLSFT
                     MGNFQNYYQLRFKQIKYEYATRLNRYFSMYGTKSNRVATPNLQTRKSWNFIKLKEPNI
                     VGTMSNDVLTRVKQIFSAGVTLWHTNDILNYNQDNGDV"
     gene            8291..9271
                     /locus_tag="IVY85_001876"
     CDS             8291..9271
                     /locus_tag="IVY85_001876"
                     /inference="COORDINATES: protein motif:HMM:NF017190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044890.1"
                     /translation="MSRRKGAGLARNNRYTAKSRPYPNEPYSSDVEEISYYEHYRRQL
                     TLLTFQLFEWENLPKSIDPRYLEIALHTNGYLGFFKDPTLGFMVCAGAEDGQIDHYHN
                     PIFFTANEAMYHKRYPVLRYDDDDDKSKCIMLYNNDLKVPTLPSLHRFALDMADINQI
                     SRVNRRAQKTPVIIQTDEKKYFSLLQAYNQIDENNQAVFVDKDMEFDESFNVWQTNAP
                     YVVDKLRSELNEVWNEVLTFLGINNANVDKTARVQTSEVLSNNEQIESSGNILLKSRK
                     EFCDRVNRVFGDELEGKIDVKFRTDAVRQLQLAAGQSKKDQMSGGLPSAT"
     gene            9261..10097
                     /locus_tag="IVY85_001877"
     CDS             9261..10097
                     /locus_tag="IVY85_001877"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044891.1"
                     /translation="MLLKRYIESFTYYHPELSRKERIEVGRKQLFDFDYPFYDETKRA
                     EFETKFINHFYLREIGSETMGSFKFNLDEYLNLNMPYWNKMFLSNLEEFPIFDDMDYT
                     IDEKQKLLNEIDTNIKANRDESKNQTKQVDQTDNRNKNTRDTGTTDSFSRNTYTDTPQ
                     KDLKIASNGDGTGVINYATNITEDLSKETTSSTGVETNNDKTNQNTRSNASEKETKNT
                     DINKDQNQTKDTITRYKGKKGSTDYADLLEKYRKSVLRIEKMIFREMNKEGLFLLVYG
                     GR"
     gene            10103..10654
                     /locus_tag="IVY85_001878"
     CDS             10103..10654
                     /locus_tag="IVY85_001878"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044892.1"
                     /translation="MVDFNPDKRFDGLPSVFKERFSKYPHTEYRYELLLDEEVSALIS
                     YLNEVGALVNDMSSYLNYFIEHFVEKLEEITTDTLKKWLSDGTLESLINDTVFANYIK
                     EIKRLQILVAETRANSVNILLTKNKPDVADDRTFWFKIQRDNTDYGNDPIDTLRIVAI
                     NKVSGWNTATGDIYLHIKGTEAV"
     gene            10654..10950
                     /locus_tag="IVY85_001879"
     CDS             10654..10950
                     /locus_tag="IVY85_001879"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044893.1"
                     /translation="MADIRTQLTSEDGSDNLFPVSKAVNIITNSGTNVEGELGSLKQN
                     NETMNTSVKNAVVTANQAKDAVTELNVNVGKLTNRVTALESTVGNLDGIRYVEV"
     gene            10952..11356
                     /locus_tag="IVY85_001880"
     CDS             10952..11356
                     /locus_tag="IVY85_001880"
                     /inference="COORDINATES: protein motif:HMM:NF023267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044894.1"
                     /translation="MTDKNIQMHDKDNNRLMPVTIAKNVLYNETNLGLTVVDIENKAN
                     EAKQTAQQAKTTADGLSTEIETATSTANQALTKAGTAQQTAEQAKTTANSASNVATTA
                     KNTADSAQKSATDLAVRVSSLENTAIQYTVLP"
     gene            11368..11790
                     /locus_tag="IVY85_001881"
     CDS             11368..11790
                     /locus_tag="IVY85_001881"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044895.1"
                     /translation="MANKNIQMKDSNDNNLYPTVRTENLLDLTSRAELTMTNCQLYTA
                     SDKTNAISYLGAIGMIEGMVKFTESLTNPVITTLPEGFRPIRTKRIGCFAKYYTPNPA
                     NTKELVYVSITPDGKVTVNDNVGKIEYLSLDNCVFPLK"
     gene            11801..13135
                     /locus_tag="IVY85_001882"
     CDS             11801..13135
                     /locus_tag="IVY85_001882"
                     /inference="COORDINATES: protein motif:HMM:NF024120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044896.1"
                     /translation="MEERIDIQMNKMKEENQKNYLLHPETNPTQVVFDETLHGNENQE
                     SFNEFVDKRKMTTTIDVSAYGVIADGVTDCTPILNKLLEEKSEKGITFYFPPCERDSY
                     YRFANTIELKPDVPIVTFLGSGETTLKFETMTAFNVNIESFNIDGFALWLPQGAQSGK
                     GIFFNDTRNYNRFDFDLFVRNCTLNEGTYVVVARGRGVTFENCLFSNISQAIIKTAFP
                     DVNSMWQGNDINTRGTGFRGFFVKHNRIHFCTAIIIDNEDDYQNVINFCEISSNTIEG
                     GVSYYRGYAHNLHVQNNNHFLAYGNRNALFEFQDVDQAYIDVDVYSRNSKVEGMNSTA
                     ISRLVVVYGHYRNLRITGKLYRCQGHVITLYGGGVNFYCDLMAQEAPLTDGYRFIQTA
                     DKRVNYDGFIVRGLSNSTKVNTPMIYKAPQTVFYNRKIDHVLTGPNASNVYN"
     gene            13543..14184
                     /locus_tag="IVY85_001883"
     CDS             13543..14184
                     /locus_tag="IVY85_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HAP8044897.1"
                     /translation="MCHWERPGKLHIEERLDLAQAYSKHIDGSGGGGVKRCYGTPIKN
                     TNLDPSSFMSGQLFGTHAGNGRPNNFHDGLDFGSIDHPGNEMIACCDGTVTHVGTMGA
                     LRAYFVINDGTYNIVYQEFSYSQSNIKVKVGDRVKNGQVCAIRDADHLHLGFTKKDFM
                     TALGSSFVDDGTWEDPLKFLGQCFGDGDNGDNNKGKNDLIYLWLSDSLNGWKF"
     gene            14202..14594
                     /locus_tag="IVY85_001884"
     CDS             14202..14594
                     /locus_tag="IVY85_001884"
                     /inference="COORDINATES: protein motif:HMM:TIGR01593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044898.1"
                     /translation="MIEYITTWLADDNHMIYGLILWLMVAMIIDFVLGFTIAKFDKEI
                     DFSSFKAKAGIIVKVAEMILVVYFIPVAVKFGAVGITMYVTMLVGLILSEIYSILGHI
                     STIDDNNNWTDYVKKFLDGTLNRKEDTK"
     gene            14594..15469
                     /locus_tag="IVY85_001885"
     CDS             14594..15469
                     /locus_tag="IVY85_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25"
                     /protein_id="HAP8044899.1"
                     /translation="MNGIDISSYQTGIDLSKVPCDFVIIKATGGTGYVNPDCDRAFQQ
                     ALALGKKIGVYHFAHEKGLEGTPEQEGAFFLKNIQGYIGKAILILDFEGSNQKDSAWA
                     KAFLDYVYNQTGIKPWFYTYTANLNTTDFSNVAKGDYGLWIAEYGRNLPQGYSQPSPP
                     RTNNFPVVACFQYTSSGRLYGYNGNLDLNVFYGDQKTWDLYVGKKSDQIVPPENKIFD
                     ATSDEFIFTLTKGSTSVFYFDGKTIFELSDPTQLDHIRGTYNHIHGKEIPSLVWTPEQ
                     FDIYLKMYDKKPVYK"
     gene            15481..16512
                     /locus_tag="IVY85_001886"
     CDS             15481..16512
                     /locus_tag="IVY85_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015991957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA encapsidation protein"
                     /protein_id="HAP8044900.1"
                     /translation="MTNSLGVKLKEKNLYYSPNNALGFNCLMLFVIGARGIGKTYGYK
                     KFVVNRFIKHGEQFIYLRRFKTELKKIPQFFKTMAKEFPEHKLEVKGKEFYCDDKLMG
                     WAVPLSTWGIEKSNEYPDVRTILFDEFLIEKSKITYLPNEAEALLNMMETVFRDRTNT
                     RCIMLSNATSVVNPYFLYFNLQPDLNKRFNLYQDRGILIELCDSKDFAEVKKQTPFGR
                     LIRGTDYEDFSINNEFVNDSDTFIEKRSKNGSFLCAIAFEGKIFGYWIDAETGCVYVS
                     YDYQPNTNHFYAMTTKDHEENRLLMKNWRNNYYLSTVAKAFKNSYLRFDNIVIKNLHY
                     DLFNKMKIW"
     gene            complement(16515..16688)
                     /locus_tag="IVY85_001887"
     CDS             complement(16515..16688)
                     /locus_tag="IVY85_001887"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044901.1"
                     /translation="MRKDFVYINTPDPKANKKALAKITNAKEPKQNYRRLQSLCYLLF
                     IIVIELIVVTLVK"
     gene            complement(16685..16996)
                     /locus_tag="IVY85_001888"
     CDS             complement(16685..16996)
                     /locus_tag="IVY85_001888"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044902.1"
                     /translation="MIARTFKEHRELIEWLRFYCKRNLSDNEKIEIIEGTLQDFDVPE
                     INITELFLTHSTLLPESSQFNILEKYCQAMNLVTSYVKVGSRYQLALQIPKGYLKEKE
                     L"
     gene            complement(17002..17292)
                     /locus_tag="IVY85_001889"
     CDS             complement(17002..17292)
                     /locus_tag="IVY85_001889"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044903.1"
                     /translation="MINNLSLILEGLNQLTKEDNDSLASIKSEITQGGNQVILYIDYV
                     TKEFVLTHDKYNYVYLDSHCINIARTKSMKSVEHYAEQLKHDGYKQITDKWE"
     gene            complement(17365..17598)
                     /locus_tag="IVY85_001890"
     CDS             complement(17365..17598)
                     /locus_tag="IVY85_001890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044904.1"
                     /translation="MFNKALIAEKGEVSLLRPFNVWDMNYHIIDTENNKHYRIDIDDV
                     GDEEYCLLSFEELKELDMDLICEYSWKTTKITD"
     gene            complement(17671..17859)
                     /locus_tag="IVY85_001891"
     CDS             complement(17671..17859)
                     /locus_tag="IVY85_001891"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044905.1"
                     /translation="MYFDILIELYDGDIEKTNLFTDTDKLKTYLYKILDKEDAEFLLE
                     NGKVVIDDYTLYLDSATY"
BASE COUNT         6568 a         2775 c         3374 g         5334 t
ORIGIN      
        1 tatctattat atcctttaca aatatgtgat tatatggtaa actatatttg taaggtaaag
       61 gggggtgaat aaaatgaaac ttacagatgc tacaaattat atgttagaac tatcatcaaa
      121 cttagaaaca attgataatt taattaaaga tcttaattct tcatacaata tgaatgatca
      181 agttcgtgtt tacttggaaa aagatgatgt cactctcgat tattgggcaa aatcaaaatc
      241 ttttaatgtt tcgtttgaaa aagaaatcat aatttcagct ttaaaagatc aaagaaaagt
      301 attatacaca gaattaataa agacgaaaga atttatctca gaactattgg aggaaaaaca
      361 atgaaaacaa gttataaaca attagatgaa ctaatttcat taaaaaatcg tataaaacac
      421 aataaaacac atcttaaata cattaccgaa tgtatagatg aagttaaaaa atcatcgggc
      481 gatccatacc gtattaaagt ttcgtacgac aatcattatc ttacacttgt ttctatcaca
      541 aaagaacaag taataaataa tttagaaaaa gaaaagttaa aattaacaac agcgatttta
      601 gaaatacaag atgaaattat agaaattttg gaggaaaaat aatgaaatac tcactagaac
      661 aaaaagatga aattaaatca acaattttca gaattagatt aaaaaggcat gaactagagg
      721 aattggtgga cgaagtaaat gatattgcta aagatccgga ggaaagatat cttttatcgt
      781 tttattacac agaagaagaa cgtttgtttg aaattccctc tgcaagatta atagattatt
      841 acaacacaaa gatcacaaat ctgaaatcgg aaatcatatc actcgaaaaa agatcacaaa
      901 agctagtaaa ataattacac aaaaagcttt acaaatataa cacatcatgt tatactaaaa
      961 gagtagtaag ggaacggaaa ataccttact tcacacctca atcattctta tcaaaataca
     1021 aaaggaggga aaataatgag tcgaaaacta atgcaacgaa acgtaacatc aacgaaagtg
     1081 gaattctcag aagttatcgt acaagacgga gcgccaacaa ttgtaccatg cgaaccagtt
     1141 gtactaacag gaaaactttc agaagaaaaa gctttatcag cgatcaaacg taaaaaccct
     1201 gataaaaacg tagtggtaat aaatgtttca catgaaacag cactttacac aatgccagtc
     1261 gataaattta tcgagttagc agacaaatca acacaagcat aataaaacaa aaacaaaact
     1321 aaaacaaaac agaggagatt ataatcatgg aaatcgtaaa aagcacattt gacacacaaa
     1381 caccagaagg aatgttacaa gtattcaatg ccacaaacgg agcttcaatt ccgttacgta
     1441 acgcaatcgg agaagtttta gaattgaaag acattctagt ttattcagac gaagtttctg
     1501 gttttggtgg cgccgaacca tcacaagcag aactagtcgc tttcttcaca gaagatggta
     1561 aaacatatgc tggggtatca gcggtagcaa caaaatcagc taaaaatctg attgatatga
     1621 tgactgctaa ccctgacatc aaaccaaaaa tttcttttgt agaaggaaaa tcaaacggtg
     1681 gacaaaaatt tgtaaatcta caagtggttt cactgtagca taaaaataca ggaatctagt
     1741 aagccactta gcgaagatcg ctaggtggtt tttattatgt ttctacattg aggtgtgtag
     1801 aattggcagt tagaatttca aagaatgata gagccaagtt agataaaatt tatggtaaat
     1861 ctaacaaagc tcgtaaaaag tacaatcgtt taagacaaaa aggagttgag gaaagacaac
     1921 ttccaactgt tccaacatca aagaaaagcc ttattgacta cgtaaaatca acaaatatga
     1981 gtcgtagtga tttcaacaag ttattagatg agttggtaga ctttgcacaa ccttacaacg
     2041 agaattacat ttttgagatc aacaagcgaa atgttgctat ctcaagagcg caaatcaaag
     2101 aagcgcatat taaaacagaa caagctcaaa aagcgaaaga agaacattac aaagagctta
     2161 acaaagttca agttaagaaa cctacagaaa acacaattgt cacaccaacc attttaacag
     2221 agttaggtgc cgacttacct tttcaagcaa taccagattt taatattgat tcttttactt
     2281 ctccagaagg ggttcagtct tatttagaaa atataggaaa acaagacgaa caatattttg
     2341 acgagaggga tcaactttat tacgacaatt tcaggcaagc gatgtttact attttcaatt
     2401 cagacgctga cgacattgtt cgtttacttg attcaatggg gcttgatcta tttatgaaaa
     2461 cctatgttag taacttctta gacatgaacc ttgactacat ttatgatgaa gcagaagtac
     2521 aacagaaaaa agaacaagtt tacagtaaga ttgcaaaagt gatcgaatct gaaacaggtg
     2581 gagaagtccc ctcatataac cccacgaaaa acatcacaat taattcagag acaggagaag
     2641 aattatgata aagaaatata caggcgactt tgaaacaaca acagatatta atgattgtcg
     2701 cgtatggtcg tggggcgtat gcgatataga caatgttgac aatataacat tcggtttaga
     2761 cattgattct ttttttgagt ggtgcgaaat gcaaggtagt gcagacatct atttccacaa
     2821 tgaaaaattt gacggagagt ttatgctttc atggttattc aaaaatggtt tcaaatgggc
     2881 taaagaaacg aaagaagaaa gaacattctc tacattgata tcaaatatgg gtcaatggta
     2941 tgctttagag atatgttgga atgtaaaatg cacaacaacg aaaacaggta aaacaaaaaa
     3001 agaaaagcaa cgaaccatca tttatgatag tcttaaaaaa tatccttttc ctgtaaaaga
     3061 aattgcagaa gcttttaatt ttcctataaa aaaaggcgaa atagattata ctaaaaaaag
     3121 acctattggt tacaatccaa ctgaagatga atgggattat ttaaagaacg atattcagat
     3181 catggcgatg gcattaaaaa ttcaattcga tcaaggacta actcgaatga ctagaggaag
     3241 tgacgcttta ggcgattaca aagattggct aaaagctaca catggaaaat caactttcaa
     3301 acaatggttt cctattttgt ctttagggtt tgataaagac ttacgtaaag catacaaagg
     3361 cggcttcact tgggtaaaca aagtttttca agggaaagaa ataggtgacg gcattgtctt
     3421 tgatgtcaac tctttgtatc cctctcaaat gtacgtaaga cctttaccat atggaacacc
     3481 tctattctac gaaggagaat acaaaccgaa caacgactat ccgctgtaca ttcaaaatat
     3541 caaagtaaga ttccgtttaa aggagggtta tattccaacc attcaagtga agcaaagttc
     3601 gttattcatt caaaacgaat atcttgaatc aagtgtaaac aagttaggag ttgacgaatt
     3661 aatcgatctt actcttacaa atgttgacct agaattattt tttgaacact acgatatttt
     3721 agagatacat tacacttacg gatatatgtt caaagcttct tgtgatatgt tcaaaggctg
     3781 gatcgataaa tggatcgaag taaagaacac caccgaaggg gctagaaaag ctaacgccaa
     3841 aggtatgtta aatagcttgt atggaaagtt tggaacaaac cctgacatca caggaaaagt
     3901 gccttacatg ggagaggatg ggattgttcg attgacacta ggagaagaag aattaagaga
     3961 tcctgtttat gttccgcttg ctagttttgt gacggcttgg ggtaggtata ctaccattac
     4021 aaccgctcaa agatgttttg acaatatcat ttattgtgat acagatagca ttcatctaac
     4081 aggaacagaa gttccagaag caatcgaaca tcttgttgat tctaaaaaac ttggatattg
     4141 gaaacacgaa agcacatttc aacgagcaaa attcattcga cagaaaacat acgtagaaga
     4201 aattgatggc gaattaaatg taaagtgtgc aggtatgcct gatcgaataa aagagcttgt
     4261 aacctttgac aattttgaag ttggtttttc aagttatgga aagttgctac cgaaaaggac
     4321 acaaggcggc gtggtattag tagatacaat gtttacaatc aaataaggag gaataataat
     4381 ggatttatat aaagcaattt ttattgtacg tgatgaagga acgattgatg ggtttgatac
     4441 cgaacactat gttgatatct ctttacatga ttttgaagaa atctacgcaa aagaaacacg
     4501 tgaaattgaa gcagtaacat tagtaaaaac aggaaatttc aaaaaataaa ttatttacat
     4561 cctttgcaaa gtatggtaaa atattcttgt gatagttaac aagagtcaaa tttggcgaga
     4621 ttgggcgaat gtacacgtga aatatcgtgc gctcccgtta agttatggac acataaacgt
     4681 tttgaccgtt aaccaatcgc aaaaaccttt taggagtagc ccttaaatgt ggctactctt
     4741 ttttgtgtgt ttcacagatt tatgtttcac gtgaaacact ttttatggta taatagaatc
     4801 aaaaggagtt gaagaatatg gaaattaaag aacacgaagc tattttaaac ggcattcttg
     4861 aaagtgtcac agacggtgaa gcaagatcaa agattgtaga acatcttgaa gcattgcgag
     4921 aagactacgg agcaacaact gaagctttga catcagcaaa tagcacactt gagaagttaa
     4981 agaaagataa cgaagcgttg gttatttcaa actcaaaatt gttccgagaa cgcgcgagcg
     5041 tagaaccgac agaaaataac gaaccagaaa cagaccagaa tattacacta gatgatctag
     5101 gaatttaagg aggaaacaaa catggcagat aaaattacag aacaagatgt tcttaacgcc
     5161 acaaatgtag aaacaccggt acaattgatg acggctattt ataatagttc atcatctctt
     5221 tttcaagcga acgtacctat gccaaatgca gataacattc aagcggttgg tgctgggatc
     5281 acacgtttag atgtagtaaa aaatgaattt atttcaactt tagttgatcg tattggtaaa
     5341 gtagttattc aatacaaatc atggcgaaac cctttgaaaa tgtttaaaaa aggaaacatg
     5401 cctttaggtc gaacgattga agaaattttt attgatattg cacaggaaca taagttcaat
     5461 cctgaagaat ctgttacagg tgtatttaaa caggaagttc ccgatgtaaa aacattgttc
     5521 cacgaaatta atcgtgaagg ttactacaaa caaacgatcc aagaagcatg gctagaaaaa
     5581 gcatttactt catgggataa tttcaatagt tttgttgccg gtgtaatgaa cgctttatac
     5641 acaggcgatg aagtaagtga atttgaatac acgaaattgt taatagcaaa ctaccaagaa
     5701 aaagagttat tcaaagagat cgaaattggc gaaattaatg aatcaaatgc aaaagaattt
     5761 attcgtaaga tcaaatcaac atcaaacaaa cttgaattta tgaacaattc ttacaatgca
     5821 caaggtgtaa gaacatcaac agctaaagcc gaccaatacg ttattattga cgctgacact
     5881 gacgcaacaa ttgacgttga cgttttagca gcagcattca acatgagtaa aacggacttt
     5941 gtaggacaca aaattgttat tgatgagttt cctaaaaaag aaggcgaaga atcttcaaat
     6001 attgtggcag ttattgtaga tagtgaatgg tttatgatct acgacaaatt gtacaaaaca
     6061 acaagtctat acaatccaga agggctatat tggaattatt ggttacacca ccaccaatta
     6121 tattctactt cccagtttgg taatgccgtt gcttttgtaa aatcatctac taaaccagtt
     6181 acaaaagttg cttttgcaag tgcaacagca agcgttgtta aaggttcatc taaagatatc
     6241 aatattacct ttacacctgt tgacgctaca aatcaacaag gagaagttgt ttcatcagct
     6301 ccatcattgg ttaaagcgac agtcaaagaa acatcaggca aagcgacagc cgtaaccgta
     6361 gaagggttag aagtcggtca atcattagta acattcacag ctatcggagg tcaacaagca
     6421 acggttcttg ttacggttac ttctgattaa ggaggacaat tatggcaaga cggtatacac
     6481 atgtaaaatt gttggctaac gtgccttttg ataacaccta tgcacacaca agatggtgta
     6541 aaactcaaca ggaacaggaa tcgtatttta attcgtttcc tgttctgaac gagaatactg
     6601 attgttctta tcaaagagat acacaactcg gtggagtttt tagagtagat aaacacaaag
     6661 acgctttata tgcttgtaac tatctcatct ttaaaaacga agaaacttat cctagtaaat
     6721 ggcagtatgc ctttgttaca gatattgaat ataagaacga caacacaagt ttcgttactt
     6781 ttgaaattga tgttttacaa acttatcgtt tcgatattgg catacgagaa agtttcattg
     6841 cgaaagaaca tccacaactt tattattcga atggagtacc attcattaat acgattgaag
     6901 agtcgcttga ttacggtaga gaatacacaa caacaaatgt aacaactttt catcctaacg
     6961 atggagtcaa ttttcttgtt attctaacaa gtgaagcaat gccagtagga gataaggaag
     7021 ataaatcagg aggatcaatt gtcggagggc cgtcacctta ttcctattat ttacttcctc
     7081 tcaattcaag tggcgaggta tacaaaccaa atggtgcagg taatgctaac tttggagagt
     7141 acatggcgtt tcttacaacg aaagaacctt ttttaaataa gatagtcggg atgtatataa
     7201 cgtcatatac aggtatacca tttattgtgg atcatgctaa caaaacagta aggtataatg
     7261 caggaggttc gtataagatt acactcccaa cctatgccag tgatccaaca ggaacaatga
     7321 aaacattcgc tttcttttgt gtgaaagagg caagaacatt tgtacctaaa agaattgatc
     7381 ttgtaggaaa tgtgtataac tactttagag aaagttttcc atttaatgtt aaggaatcta
     7441 aactatttat gtatccttat tgtttactag aaattacaga tactaaagga catgttatga
     7501 ctatcagacc cgaatatctt acaggtggta aattgagtgt atatgtaaaa ggttcgttag
     7561 gcatttctaa taaagtcatg attgaaccaa ttgattatga tgtaactaac tcatccatta
     7621 ttaccaattt aagtgacaag atgttaatcg ataatgatcc taatgatgta ggagttaaat
     7681 ctgactatgc tgctgcattc atgcaaggga acaaaaactc cttgattgct caagaacaaa
     7741 acatacgaaa cacctttagg catggtatgg gaaacagtgc aatgagtaca ggaggggcga
     7801 tcttttccgc attagccagc gccaatccat ttgttggttt gactaacatt atgggggctg
     7861 gacaacaagt aaacaactat gttgctgaaa aagaaaatgg attaaatctt ttggcaggca
     7921 aagtagcaga tatcgaaaat atcccagata atgtcacaca gctaggatca aacttatctt
     7981 tcacaatggg aaactttcaa aattattatc aattgcgatt caaacaaatt aaatatgagt
     8041 atgctacaag actgaatcgt tacttctcaa tgtatggcac aaagagcaat cgagtagcta
     8101 caccaaactt acaaacaaga aaatcatgga atttcattaa attaaaagag ccaaatattg
     8161 taggtacaat gagtaacgat gtgttaacgc gagtgaaaca aatttttagt gcaggagtta
     8221 ccctttggca tacgaacgat attttaaatt ataaccaaga caacggagat gtgtaggaag
     8281 gaggaataag atgagtagac gaaaaggtgc aggacttgct agaaataacc gttatacagc
     8341 aaaaagcaga ccttatccaa acgaacccta ttcaagtgat gtagaagaaa tcagctacta
     8401 tgaacattat cgtagacaac tcacgctcct tacgtttcag ttgtttgaat gggaaaattt
     8461 accgaaatca attgaccctc gatatttaga aattgcatta cacacaaatg ggtatctcgg
     8521 tttctttaaa gaccctacac tagggttcat ggtttgtgcc ggtgcagaag acggtcaaat
     8581 tgatcattat cacaatccta tattctttac cgcaaacgaa gcaatgtatc acaagagata
     8641 tcctgtttta aggtatgatg atgatgatga taaatcaaaa tgtatcatgt tgtataacaa
     8701 tgacttgaaa gttcctacat taccaagtct tcatcgtttt gctttggata tggcagatat
     8761 aaaccagata tcacgagtga accgaagagc gcaaaaaaca cctgtaatta ttcaaacaga
     8821 tgaaaagaaa tatttttcgt tgctacaagc ttataatcaa attgatgaaa acaatcaggc
     8881 tgtttttgtg gataaagata tggagtttga cgaatctttt aatgtatggc aaacaaatgc
     8941 tccatatgtg gtagataaac tacgatccga attgaatgaa gtatggaatg aagtgttgac
     9001 ttttttaggt attaacaatg caaacgttga taagactgca cgtgtgcaaa catcagaagt
     9061 cttatctaat aacgaacaga ttgaaagttc aggcaacatc ttgttaaagt cgagaaaaga
     9121 gttttgcgat cgtgtaaatc gtgtctttgg tgacgaactt gaaggaaaga ttgatgtgaa
     9181 gtttagaaca gacgctgttc gacaattaca actagctgca ggtcaatcga aaaaagacca
     9241 aatgagtgga gggttgccaa gtgctactta aacgctatat tgaaagtttc acttattacc
     9301 atcctgaatt atctcgaaaa gaacgtattg aagttggcag aaaacaattg tttgattttg
     9361 attatccttt ttatgacgaa acaaaacgag ccgaatttga aacaaaattt atcaatcact
     9421 tttacttgag agagatagga tcagaaacga tgggatcatt taagtttaat cttgacgagt
     9481 atttaaatct aaacatgccc tattggaata agatgttcct atcaaatctt gaagagtttc
     9541 cgattttcga tgacatggac tataccattg atgagaaaca gaaattgtta aatgagattg
     9601 atacaaacat caaagcgaat cgggatgaat cgaaaaatca aacgaagcaa gtcgatcaaa
     9661 cagacaacag aaacaaaaat acacgtgaca caggaacaac cgattctttc tcaagaaaca
     9721 cgtatacaga cacccctcaa aaagatttaa aaattgcttc aaatggcgat ggaacaggcg
     9781 tcatcaatta tgcaacaaat atcacagaag atttaagtaa agaaacaaca agttctacag
     9841 gtgtcgaaac caataacgac aaaacaaatc aaaacacacg aagcaatgct agtgaaaaag
     9901 aaacaaagaa cacagacatt aataaagatc aaaatcaaac aaaagatacg attacacgat
     9961 ataaaggtaa aaaaggaagc accgattatg ctgacttact cgaaaaatat cgcaaaagtg
    10021 ttttgagaat tgagaagatg atctttagag aaatgaacaa ggaaggatta tttctccttg
    10081 tttatggagg gaggtagtag caatggtaga ttttaacccc gacaagcggt ttgatggttt
    10141 accctcagta ttcaaagaac gttttagcaa atatcctcat actgaataca gatacgaatt
    10201 actattagat gaagaagtat cagctttaat ttcctatctg aatgaagttg gcgctttagt
    10261 taatgatatg agtagttatt taaattactt tatcgaacat tttgttgaga agttagagga
    10321 gatcacaact gacacactca aaaaatggtt atctgacggt acactagaaa gtttaatcaa
    10381 cgatactgtt tttgcaaatt atatcaaaga aattaaaaga ttacaaatct tggttgctga
    10441 aacaagagcg aacagtgtga atatcctttt gacaaagaat aaacctgatg ttgctgatga
    10501 tcgaacattt tggtttaaaa tacagcgtga taatactgat tatggtaatg atcctattga
    10561 cacgttacgt attgttgcaa tcaataaagt tagcggttgg aataccgcta caggagatat
    10621 ttatcttcat attaaaggaa cggaggctgt ataatggcag acattagaac acaactaaca
    10681 agtgaagatg gatcagacaa tttatttcca gtttcaaaag ccgttaatat tataactaat
    10741 agcggtacga atgtagaagg agaattgggt tcactcaaac aaaataacga aacaatgaat
    10801 acctcagtta aaaatgctgt agttactgct aatcaagcaa aggatgctgt aactgaatta
    10861 aatgtaaatg ttggtaaact aaccaataga gtaacagcat tagagagtac agtcggtaat
    10921 cttgatggta ttcgttatgt agaggtgtaa tatgacagat aaaaatattc aaatgcatga
    10981 taaagataat aatcgtttaa tgcctgtaac cattgcaaaa aatgttttgt ataatgaaac
    11041 taatttaggg ttgactgttg ttgatattga gaacaaagct aatgaagcaa aacaaacagc
    11101 tcaacaagcg aaaacaacag ccgatggatt gtcaacagaa attgaaacag caacttcaac
    11161 cgccaatcaa gcgttaacga aagctggtac agcgcaacaa accgcagagc aagcgaaaac
    11221 aacagcgaac agtgctagta atgttgcaac gacagctaaa aacaccgctg attcagcaca
    11281 aaaaagtgca actgatcttg ctgttcgtgt aagtagttta gagaacacag caatacaata
    11341 tactgtatta ccgtaggagg aaaaataatg gcaaataaaa atattcaaat gaaggatagc
    11401 aatgacaata atttatatcc aactgttcgt acagaaaact tgttagattt aaccagtcgt
    11461 gctgaattga caatgacaaa ttgtcaatta tatacagcta gtgataaaac aaatgcaatc
    11521 tcttatctcg gagcaattgg tatgatcgaa ggtatggtaa agtttactga aagtttgaca
    11581 aaccctgtga ttacaacatt gccagaaggt tttagaccaa taagaacaaa acgtattggt
    11641 tgtttcgcaa aatactacac accaaatcca gcaaatacaa aagaattggt ttatgtgtcg
    11701 atcacacctg atggcaaagt aactgtaaat gacaatgtag gtaaaatcga atatctatca
    11761 ttagataatt gtgttttccc tctaaaataa ggaggttcat atggaagaac gaattgatat
    11821 tcaaatgaac aagatgaaag aagaaaatca aaagaattac ctattgcacc ctgaaacaaa
    11881 tccaacacag gttgtttttg atgaaacatt acatggaaat gaaaatcaag aaagtttcaa
    11941 cgaatttgtt gacaaaagaa aaatgacaac tacaatcgat gtaagtgctt atggggttat
    12001 tgcagatggt gtaacagatt gtacaccaat attaaataaa ttactcgaag agaaaagcga
    12061 aaagggtatc actttttatt ttcctccttg tgaacgtgat tcatattatc gctttgctaa
    12121 cacaattgaa ttaaagcctg atgttccaat agtgactttc ttaggatcag gagaaacaac
    12181 attaaagttt gaaacaatga cggcatttaa tgtaaacatc gaaagtttca acattgatgg
    12241 ttttgcatta tggttgccac aaggcgctca aagtggtaaa ggaattttct ttaatgatac
    12301 tcgaaattac aatcgtttcg actttgattt gtttgttcgc aactgtactt taaatgaagg
    12361 aacatatgtt gttgttgcta gaggtagagg ggttaccttt gaaaattgtc tattctctaa
    12421 tatctctcaa gcaatcatca aaacagcttt tcccgatgta aacagtatgt ggcaagggaa
    12481 cgatattaat actagaggca caggatttag aggatttttt gtgaaacaca atcgtattca
    12541 cttttgtaca gcgatcatta ttgataacga agatgattat cagaatgtaa ttaatttctg
    12601 tgaaatttct agtaatacga tcgaaggtgg cgtaagttat tatcgaggat atgcacataa
    12661 cttgcatgtt caaaacaaca accattttct agcatatgga aatagaaacg ccttgtttga
    12721 atttcaagat gtggatcaag cttatattga tgtagatgtt tattctcgta actctaaagt
    12781 tgaaggaatg aatagtacag ctatttcacg tttagttgtt gtttacggac attaccgaaa
    12841 cttaaggatt acaggaaagt tatatcgttg tcaaggacat gttatcactt tatatggtgg
    12901 tggcgttaat ttctattgtg atctaatggc acaagaagca cctttgacag atggttaccg
    12961 ctttattcaa acagcagata agcgtgttaa ttatgatggg tttattgttc gtggattgtc
    13021 taattcaaca aaagtaaata ccccgatgat ctataaagca cctcagactg ttttctataa
    13081 tcgtaaaatt gatcatgtac taacaggacc aaatgcaagt aacgtatata actaggagga
    13141 tatgagatgg caactcttac aaatgaacaa atagctagag gacaaacaat tgctaaaata
    13201 cttggtaaac atggctataa taaaaattca caagtaggag ttgtcgctaa tctccattgg
    13261 gaatcggctg gattgaaccc gaacgccaat gaatacggtg gcggtggtta tggcttaggt
    13321 caatggacgc ctaaaagcaa tctttatcgc caagctcaaa tttgtggatt gtctaatgct
    13381 caagctgaaa cgttggaggg tcaagcagag atcatcgctc aaggagataa aacaggtcaa
    13441 tggatggata atacacctat ttcttatcca ggatatacta atcctcagac cctctcggca
    13501 tttaagcaat ccacaaatat tgatgttgct acaatcaatt ttatgtgtca ttgggaaaga
    13561 ccaggcaagc ttcatattga agaaagactt gatcttgcac aagcttatag taaacatatt
    13621 gatggtagcg gtggcggtgg cgtaaaacgt tgctatggaa caccgatcaa gaacacaaat
    13681 ctcgatccta gtagtttcat gagtggacaa ctttttggta cacacgcagg aaatggcaga
    13741 ccaaacaatt tccatgatgg tttggacttt ggttcaatcg accatccggg taatgaaatg
    13801 atcgcttgtt gcgatggaac agtaacacac gttggaacaa tgggcgcatt aagagcgtat
    13861 tttgtgataa atgatggtac ttacaatatc gtttatcaag aatttagtta tagccagtca
    13921 aacataaaag taaaggttgg cgatagagtt aagaatggtc aagtgtgtgc tatacgtgac
    13981 gcggatcatt tacacttagg gttcacgaaa aaagacttta tgacagcgtt aggatcatct
    14041 tttgttgatg atggaacgtg ggaagaccct ctaaaatttt taggacaatg ttttggcgat
    14101 ggagataatg gcgacaacaa taagggtaaa aatgatctta tttatttgtg gctatctgat
    14161 tcattaaatg gctggaaatt ttaataagga gaaaaaatgg tatgatagaa tatattacaa
    14221 catggttagc agatgataac catatgattt acggtttaat tttgtggcta atggttgcga
    14281 tgattattga ttttgtgtta ggttttacaa ttgccaaatt tgacaaggaa atcgacttta
    14341 gtagttttaa agctaaagca ggaattattg ttaaggtggc agaaatgatt ttagtggttt
    14401 actttattcc tgtagcagta aaattcggtg cagtaggtat tacaatgtat gtaacaatgt
    14461 tggttggttt aattttatca gaaatttata gtatactagg acacatttca actatagacg
    14521 ataataacaa ttggactgat tatgttaaga agtttttaga cggaacactc aacagaaagg
    14581 aagatactaa atgatgaatg gtattgatat ctctagttat caaacaggga ttgatctttc
    14641 aaaagttcca tgtgattttg taattattaa agcaacaggg ggtacagggt atgtcaatcc
    14701 tgattgtgat cgagcatttc aacaagcttt ggcattaggt aaaaagattg gtgtgtacca
    14761 ctttgcacat gagaaaggct tagaaggtac accagaacaa gaaggagcat ttttcttaaa
    14821 gaatattcaa ggttacattg gcaaagcaat tctaattctt gactttgaag gttcaaacca
    14881 aaaagattca gcatgggcga aagcatttct tgattatgtg tacaatcaaa caggcattaa
    14941 accatggttc tacacgtata cagcaaacct caatacaaca gacttttcaa atgtagctaa
    15001 aggcgattat ggattatgga ttgcggaata tggtaggaat ttacctcaag gatatagtca
    15061 acctagtcca ccacgaacaa ataactttcc agttgtcgct tgttttcaat atacgagtag
    15121 cggtcgccta tatgggtata atggaaacct tgacttaaat gtgttttatg gggatcaaaa
    15181 aacatgggat ttgtatgttg gtaaaaaatc agatcaaatt gttcctcctg aaaataaaat
    15241 atttgatgct acaagtgatg agtttatttt cacattgaca aagggtagta caagcgtgtt
    15301 ttattttgat ggaaaaacga tctttgaatt gtctgatcca acgcaactcg atcatattcg
    15361 tggaacgtac aatcatatac atggaaaaga aattccatca ttggtgtgga cacctgaaca
    15421 gtttgatatt tatttaaaaa tgtatgataa gaaaccagtg tataaatagg agtgtatagt
    15481 atgacaaata gcttaggcgt taaacttaaa gagaaaaact tatactatag tcctaacaat
    15541 gctttaggtt tcaattgcct aatgttgttt gttatagggg ctcgtggtat agggaaaaca
    15601 tatggatata agaaatttgt tgttaatcgt tttataaaac atggcgaaca atttatttat
    15661 ttaagaagat tcaaaacaga acttaaaaag attcctcagt ttttcaagac aatggcgaaa
    15721 gaatttcccg aacataaact tgaagttaaa gggaaagagt tttattgcga tgataaatta
    15781 atgggttggg ccgttccgct tagtacatgg ggtattgaaa aatctaatga atatccagat
    15841 gttcgtacaa ttttatttga tgagttttta attgagaaat caaaaatcac ttatttaccg
    15901 aatgaagctg aagccttgtt gaatatgatg gaaactgttt ttcgtgatcg gactaatact
    15961 agatgtatta tgttgagtaa tgcaacaagt gtagtcaacc cttatttctt gtatttcaat
    16021 ctacagccag atttgaataa gcgttttaat ctatatcagg atcgaggaat tttgattgaa
    16081 ttgtgtgatt caaaagactt tgcagaagta aagaaacaaa cacctttcgg aaggttaata
    16141 cgtggtacag attatgaaga ttttagtatc aacaatgaat ttgttaatga tagtgataca
    16201 tttattgaaa agagaagtaa gaatggcagt ttcttgtgtg caattgcttt tgaagggaaa
    16261 atatttggtt attggataga cgcggaaacg ggttgtgtat atgtgagtta tgattatcaa
    16321 ccaaatacaa accattttta tgcaatgact acaaaagacc atgaggaaaa cagattgttg
    16381 atgaaaaatt ggcggaataa ttattatctt tcaacagtgg cgaaagcctt taagaatagt
    16441 tatttaaggt ttgataacat tgttattaag aacttacatt atgatttgtt taataagatg
    16501 aaaatctggt aaccctattt tactagagtt accacgatta gttctattac aatgatgaat
    16561 agtagataac atagtgattg tagtctacga tagttttgtt ttggttcttt ggcgttagtg
    16621 atttttgcta acgccttttt atttgctttt ggatcaggtg tgttaatgta gacgaaatct
    16681 tttctcatag ttctttctcc tttaaatatc cttttggtat ttgtaacgct aactgatagc
    16741 gagaaccaac ttttacgtat gaagttacta ggttcattgc ctgacaatac ttttcaagta
    16801 tgttaaattg acttgattcg ggcaatagtg tcgaatgagt taaaaaaagc tcggtgatat
    16861 ttatttccgg aacgtcgaaa tcttgtaaag taccctctat gatctctatt ttttcattgt
    16921 ctgaaaggtt acgtttacag tagaaacgta accattcaat aagttcgcga tgttctttga
    16981 atgttcgtgc aatcatttta atcactccca tttatccgta atttgtttat atccgtcatg
    17041 tttcaattgt tccgcatagt gttcaacgct tttcattgat ttcgttcttg cgatattaat
    17101 gcaatgacta tcaagataaa catagttata tttatcatgt gttaacacga actcttttgt
    17161 aacgtaatca atgtataaaa ttacttggtt tcctccttgt gttatttctg acttgataga
    17221 cgctaaacta tcgttgtcct ctttagttag ttgatttaac ccctctaaaa ttaatgataa
    17281 attgttaatc atgtaaaaca ctccttttat attaatttga tattgatacc accaatcaaa
    17341 taagattggt agcattgtat taaattaatc agttattttt gtagttttcc atgaatactc
    17401 gcaaataaga tccatatcta attcctttaa ctcttcaaaa gataacaaac aatattcctc
    17461 atcgcctaca tcatcgatat ctatacgata atgcttattg ttttcggtat ctatgatatg
    17521 ataattcata tcccagacat taaaagggcg aagtagagat acctctcctt tttcagctat
    17581 taatgctttg ttaaacatat gaaacactcc ttttatatta atttgatatt gataccacca
    17641 atcaaatgtg attggtagca ttgtatcgaa ttaataggtt gcgctatcaa gatacaacgt
    17701 gtaatcatca ataactacct ttccgttttc taataaaaat tcggcgtcct ccttgtctaa
    17761 aattttgtaa aggtaagttt ttaatttatc tgtatccgtg aataggtttg ttttttctat
    17821 gtcgccgtcg tataattcaa ttaaaatatc aaaatacatt tttatcatcc tttctttatt
    17881 acatctatat tatatcatgt atctacatat atgtcaatca tttaattcat ttatttaatg
    17941 atttatttga ttgtttttat gatcctttct ttattacata tatagtatat catgcatgtt
    18001 tgtatttgtc aacaattaaa ttcatataaa tgtagtttgg ggtcagttac a
//
LOCUS       DACCYH010000058        17727 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.059, whole genome shotgun sequence.
ACCESSION   DACCYH010000058 DACCYH010000000
VERSION     DACCYH010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.059"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(51..3704)
                     /gene="rpoC"
                     /locus_tag="IVY85_001892"
     CDS             complement(51..3704)
                     /gene="rpoC"
                     /locus_tag="IVY85_001892"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP8044906.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3783..7400)
                     /gene="rpoB"
                     /locus_tag="IVY85_001893"
     CDS             complement(3783..7400)
                     /gene="rpoB"
                     /locus_tag="IVY85_001893"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8044907.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8128..9096
                     /locus_tag="IVY85_001894"
     CDS             8128..9096
                     /locus_tag="IVY85_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8044908.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9114..10019
                     /locus_tag="IVY85_001895"
     CDS             9114..10019
                     /locus_tag="IVY85_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8044909.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10016..10822
                     /locus_tag="IVY85_001896"
     CDS             10016..10822
                     /locus_tag="IVY85_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8044910.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10979..11947
                     /locus_tag="IVY85_001897"
     CDS             10979..11947
                     /locus_tag="IVY85_001897"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP8044911.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12264..12788)
                     /locus_tag="IVY85_001898"
     CDS             complement(12264..12788)
                     /locus_tag="IVY85_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP8044912.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13075..14886
                     /gene="scm"
                     /locus_tag="IVY85_001899"
     CDS             13075..14886
                     /gene="scm"
                     /locus_tag="IVY85_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP8044913.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(15041..16027)
                     /locus_tag="IVY85_001900"
     CDS             complement(15041..16027)
                     /locus_tag="IVY85_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8044914.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(16050..16616)
                     /locus_tag="IVY85_001901"
     CDS             complement(16050..16616)
                     /locus_tag="IVY85_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP8044915.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(16630..17046)
                     /locus_tag="IVY85_001902"
     CDS             complement(16630..17046)
                     /locus_tag="IVY85_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP8044916.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(17503..>17727)
                     /locus_tag="IVY85_001903"
     CDS             complement(17503..>17727)
                     /locus_tag="IVY85_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase FAD-containing subunit"
                     /protein_id="HAP8044917.1"
                     /translation="LFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRA
                     IGLDRWVIPWLQRTLGNWWYGRPSSQYGKK"
BASE COUNT         5098 a         3832 c         3460 g         5337 t
ORIGIN      
        1 caaaaaagag gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt
       61 tgattgttca ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca
      121 ttttcgcttg ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta
      181 ccagctggga tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc
      241 ttaccgcgga tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat
      301 aagaaactat ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt
      361 gcaggaacgc ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct
      421 aataatgtac ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc
      481 atttgacgaa ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa
      541 acacgttgta cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac
      601 aattgttttg gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct
      661 tctgctactt tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct
      721 ttgattgtta cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct
      781 gttacttctg tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca
      841 cgaggaagac cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc
      901 gtcaactgag taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg
      961 acttcaacgt cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt
     1021 tttgtattac atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt
     1081 tgttttgcga tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca
     1141 ggatgacgta cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg
     1201 atttcatttc cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct
     1261 tcacggatga ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg
     1321 gcagtcttca aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt
     1381 tccaagacgg atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca
     1441 ttaggagcgg ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca
     1501 cgagctccag aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt
     1561 ttttgttgga tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg
     1621 tcatctgtga tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca
     1681 ttttcaatga tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata
     1741 ccggcatgtg tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt
     1801 tctgttactt tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg
     1861 aacggtaata ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca
     1921 aagtatttgt ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg
     1981 aattcaggag gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt
     2041 tctttttgcc attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt
     2101 gaatgtaaat gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag
     2161 accattcctt cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc
     2221 atatcttggg aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca
     2281 gctaacatca acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc
     2341 atttggtctc catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt
     2401 gcgcgtcctt gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt
     2461 gcccggttca gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg
     2521 tcttcttgac gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga
     2581 acaagctcac gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca
     2641 cattggtaca tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca
     2701 cgtttaccta gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga
     2761 gatttcaatg ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc
     2821 aatgcatcta ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca
     2881 ttcaagtcta acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca
     2941 ttcaagtcac ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt
     3001 ggaataacag ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat
     3061 gcttctaaaa tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc
     3121 aattcttctt tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta
     3181 atcgcttctg cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga
     3241 tattcgcgtt ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact
     3301 acatatgaag caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga
     3361 cccatacgtg aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca
     3421 atatgtccca tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca
     3481 caaacgattc ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt
     3541 gtaggaccaa aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag
     3601 ttaatcgtct caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa
     3661 gccaaaccta tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat
     3721 ttcatttaat ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg
     3781 gattagtcta aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct
     3841 tccacttgtt cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt
     3901 gtcaaagcat caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc
     3961 tctgtttctt cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc
     4021 aatacgcgga atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct
     4081 tcgtatgttt tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa
     4141 gtataagccg caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca
     4201 ccaaattgag ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa
     4261 cgtgcatgca atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca
     4321 gacacacgtc catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg
     4381 ctggccatac cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat
     4441 actggcgttg ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat
     4501 acttgcccga tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt
     4561 gttccatcag gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta
     4621 tttccgtgac gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa
     4681 acacgaacta acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt
     4741 ttcacatcgt gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact
     4801 tcacgagctt tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt
     4861 accccttttg gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata
     4921 cggatgatac ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg
     4981 cgtgtgattt cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata
     5041 tggatagaag tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca
     5101 tagttgtacc cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg
     5161 ccttgttcca tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa
     5221 tgaacgattg gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca
     5281 tcgtatttat ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat
     5341 tcaacgacac cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat
     5401 tccataccag taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc
     5461 atgttggcac ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct
     5521 gtcgcaacgg caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat
     5581 acttctaagt tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct
     5641 tcattcaatg ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag
     5701 tagtcgattt ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg
     5761 ataaatccga atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt
     5821 gggccttcag gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga
     5881 acttcataac cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt
     5941 ttgtgtgtca attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa
     6001 ccaaagaatt ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc
     6061 aatgtttcag tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc
     6121 ccgatacgga attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag
     6181 tggtcgatat catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa
     6241 gcaatgatgt cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc
     6301 acgtttactt cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt
     6361 ggatctgtga ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca
     6421 atgaaaggag ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg
     6481 atctcgcctg tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt
     6541 ttcaaatcta attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt
     6601 ggatcgaaga aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct
     6661 ggacgcaaac gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt
     6721 ttatgcaagt ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag
     6781 atcgtatcat cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt
     6841 gtacggtcga tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct
     6901 ccacggttag ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca
     6961 tggaaataga cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg
     7021 atgatgaatg ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct
     7081 ttgatttcgc cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag
     7141 tttgcatcat gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct
     7201 acaaattcca aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt
     7261 tctctaagtc cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt
     7321 ggtaattcca acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat
     7381 tttactacgt gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc
     7441 gatcaattgt tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc
     7501 ggcttttagg caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc
     7561 ttctactttt gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc
     7621 ttcatcagat agtttttgca acttcatata ctacacttat tttctataaa agtcaacact
     7681 tcctggcgtt tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc
     7741 aattatctga caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc
     7801 attttgtgga ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa
     7861 taacttttac tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta
     7921 aacgacagtt tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag
     7981 acaaaatgct aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt
     8041 tttcaattta ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg
     8101 tttcccaaaa ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct
     8161 gccctgctac tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat
     8221 ggacaaaaaa caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat
     8281 aaaggattca aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc
     8341 gattatcaaa atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc
     8401 aaagaagatc ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat
     8461 gaaacaacca ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta
     8521 gtcacatcca atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc
     8581 gaaaaacaaa tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg
     8641 tataacaacg gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa
     8701 aaagcgggcg tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg
     8761 acgaaaagtc tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca
     8821 agtgcggcaa gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc
     8881 gcagttgatc aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg
     8941 ggtaaacaaa cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt
     9001 cctgtcgaaa cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta
     9061 ggcatcaatc cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa
     9121 atttgctctc tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga
     9181 ttggtgtata cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct
     9241 ttcctttagg tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag
     9301 cttgtcttct aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca
     9361 caaaattgaa aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg
     9421 tcacatcccg tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc
     9481 attgggtcga gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt
     9541 ttgctttgat cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta
     9601 ttcgtgcaac tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata
     9661 tgaagatcat cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg
     9721 cgcaaaacaa cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg
     9781 cttctattat catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga
     9841 ctgtcgtttt aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg
     9901 ttgaaccaag tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc
     9961 cgcagatcca gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa
    10021 tgacgtctta acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa
    10081 tcatgtcctg aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg
    10141 tggaaatggg gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga
    10201 tcagggtcaa attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc
    10261 gaaatccatc agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt
    10321 agaagaaaat cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt
    10381 caaaccacaa gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga
    10441 aaatcgttta tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt
    10501 attgatggcc acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct
    10561 tgaccctgct acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa
    10621 tctcactgct tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact
    10681 gatcatgctg catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct
    10741 cacagtgccc caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga
    10801 tcaagtactt ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct
    10861 cttctttttt ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga
    10921 aaactatttt gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat
    10981 gacaacgaaa gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga
    11041 aaagttggca cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt
    11101 ggaaaaggca ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga
    11161 cgtactagat gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt
    11221 acaaacgatg ttgacttctg aatctacgat gaaagatgtc aaactggcag cagctcaagg
    11281 agattctgtt tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg
    11341 acggcctttt ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga
    11401 caaacggttt gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc
    11461 cattgaatct gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga
    11521 tggaaaaaaa gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat
    11581 cagtcatatc atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc
    11641 tgatctccaa gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa
    11701 tcagctaatc agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc
    11761 gttggacttc tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa
    11821 aggaacgact tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca
    11881 aacaaatgaa cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac
    11941 gcaataatta tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga
    12001 attctcttca aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa
    12061 aggtattcta aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa
    12121 aacaagagag tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat
    12181 cttatagata gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg
    12241 taagttcttt tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc
    12301 acctgaccag agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg
    12361 actggtggtg tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt
    12421 aagtaagaaa tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga
    12481 ttagcttgga tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct
    12541 atcgtcatcg gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct
    12601 aaatcagcaa tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat
    12661 ttgatctgcg gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct
    12721 tcaaacaaac gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa
    12781 taatccattt attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata
    12841 cctacttata tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat
    12901 agtttggaga ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat
    12961 atgaacgttt cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga
    13021 ctaggagaaa aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa
    13081 aaaagggcaa gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg
    13141 gccgtttcag cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact
    13201 ggtaactatg gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca
    13261 actgtgaatg cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat
    13321 tttggtacag agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact
    13381 gattctttcg ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc
    13441 gttggcagta acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg
    13501 cttgatcact taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat
    13561 ttcgagcagg aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca
    13621 gctaaaacat atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact
    13681 gatttatcag ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat
    13741 acgccacttc aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt
    13801 tcttctgcat tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca
    13861 gaagattttt cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca
    13921 atcagtgctc cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa
    13981 aaccaagacg gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg
    14041 gatcctaacg aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt
    14101 acagatacat ctaccacaag tgatagctca gattcatcaa cttcaagcga cagtacagat
    14161 actacctcta ccacaagtga tagcacggat acatcagctt caagcgacag tacagatact
    14221 acctctacca caagtgatag cacggacaca tcagcttcaa gtgacagtac agatactacc
    14281 tctaccacaa gtgacagcac ggacacgtca gcttcaagcg acagtacaga tactacctct
    14341 accacaagtg atagcacgga cacatcagct tcaagtgaca gtacagatac tacctctacc
    14401 acaagtgata gcacggacac gtcagcttca agcgacagta cggatacatc taccacaaca
    14461 agtgatagca cggacacgtc agcttcaagt gacagtacag atactacctc taccacaagt
    14521 gatagctcag attcatctac cactacaagt gatagcacgg acacgtcagc ttcaagtgac
    14581 agtacagata catcctctac cacaagtgat agctcagatt catcaacttc aagcgatagt
    14641 acagacacgt cgtctaccac aagcgataca cgtcatgcaa gtgtctcctt ctcaaaaaca
    14701 agttcggata acaggacgaa ctttaactta actaataaaa caagcaatga caatggaaac
    14761 aaatcaaaaa gagcacttcc taaaactgga agtcaatcga ataattggat aacactcgca
    14821 ggcgttatat tacttgtaat cggtttaaga atcactttta tgagttataa gaaatcgaaa
    14881 aggtaaaaaa atattcaaga taaaaaagag caacgtcgct aacggacgtt gctctttatt
    14941 tttgcttttt atcataaata caagaactac aattcaattt gtcaaaaaac acgtagattt
    15001 ccttctgaga aatttattta cgtatcgaac ctttccctat tcagtctttg cgatccaaaa
    15061 tcagttgggt aagggaaaag agattctctt ttgttcgaag agaggtggca ggtaatttct
    15121 ttatttcctt caaagccttt tctgtgtaag aggccgctaa cttcctagct tcttccacgc
    15181 ctcctgtatg ttcaacgatt ttgcgtacgg cgtcgacatc ttcttctgtc attgccgctt
    15241 tcttctctaa ataaggtagt agctttggtt tgtttttttg caaagagtag atcaacggta
    15301 aagaatagac cccttgacgc atgtcttcta gtacaggttt gccgatttcc tggctctctt
    15361 ttgtataatc taaaatatca tcgataatct gaaaggccat cccgatagaa agtccgatat
    15421 ctcctgcttt ttttgcgaag cgctcgcttg ttccactttc aaaagcgcca acagaacaac
    15481 ttaattggaa cagttcagct gtcttacctg aaatattttc caaatattct tttacagaaa
    15541 gttcataatt atagcgatca tccatctgcc ctaattcgcc attcaatact ttttccatac
    15601 tgcgagaatt caattggaga cttttcaagg aagtggagta atcagacaat agtttaaaac
    15661 agcagacaaa taaatagtct cctgcataga cagaaacgac attaccaaac tgactacgga
    15721 tattaggctg accacgtcgc agatccgctt catcaacgat atcatcatgg atcagtgtgg
    15781 ccgtatggag catttcgata gaagctgcca aagcgatggc tttgtctcga tcttgctctg
    15841 gtccaaattg tgagaacaat agctgatagg caggacgcaa cagctttccg ccggcatgga
    15901 tcgtttcttt gattgcttgt tcgacaggtt tgtttttcag gcggacagtt tcttccatcc
    15961 gttttagtgt ttctgtcaat tctttactta aagcaggata gttttcccac attggatgta
    16021 gcttcataaa aaaactcctt gtgtgacgat tattttttgg taaacattgg gctccatttt
    16081 tgtttggttc cctgctcata atctttttgg aatcgttgaa gtgtctgcac atgtcgtaaa
    16141 agataattcg ctgcgatccc gattgcaatc cctgataaaa tccctaaaac tgaaaggact
    16201 ggcaagtaca acatgactgt ccatgattgt gcaatgaaag aagccatcaa aagctgtccg
    16261 acattatgca tgaaaccgcc ggccgcacta attccgatga tactgacacg ttttggtcct
    16321 aattgcttga ccaataacat tccaaaataa ctaagcaaag caccactcat actataaagg
    16381 aatgtggaaa tcgttccgcc tagcaaagta gtcaggaata gtcgtagcca aagtagtaag
    16441 aagctgtctc gttttttcat ggtaaaaatg gcaacgatcg tgattagatt ggctaagcct
    16501 agttttgcac caggcgcaaa agaaaaagga taagggatca tattttccaa taaaccaatg
    16561 atcactcctt gtgcaacaag tagcgaaata taaatcattt tttgtaattt actcatggtg
    16621 tgtgaaggtc taataaatca gactgtcttc gtcacctcca tctgttgaac ggacttcgat
    16681 cactaattta tgtggcaaac agacgatggt ttcgccattt ttcgttgccc agcctcgacg
    16741 tacgcagatc tggtcatcgc aatctgcttc ttttatccgt actcgatcgc catctatctc
    16801 gatcaagtta taatctccgt gttcatcttt gtaagtatat gtgttgctct cttctccagc
    16861 catcaaaggg aatgttttga tttctgtgcc atccacacgc agtaccgctt ctttttctgc
    16921 tgttgcggtg ttttgctgaa gaccaaaaat catgactggt aaaaaagaaa gaacagtcaa
    16981 aagcaaaata ataaaaacat cccaaggttt gagcagactt ttttcagcaa attctttaag
    17041 gctcattttt gttctccttt ttccagatta actcacccaa ttttagcata aaaacaagag
    17101 gttgtgacac ctgtgtcttg ctttgagtaa taagaacgaa gaatagaaaa cagctttcca
    17161 tgttttctaa ttctttgggc ttattaccga gaagcagcag gtggaacacc gtttataaca
    17221 agaggttgtg acagaagtgg gcaacttcaa gaaataagaa ggaatttacg aaaattactt
    17281 ttcaagtttt tgtgaaattt cggcttattt ttcgaagaag ctacttctgg aacaccgttt
    17341 ataacaagag gttgtaacag aggttgtgac acctgtgtct tgctcatgga ggcttatctc
    17401 gggcatctta ttcctttaat tttacataaa aaggaggctg tgacggtttg tctcagcccc
    17461 tttctattga aatcttgcat tcaaataacg ttctttatct atttatttct tgccgtactg
    17521 ggaacttggt ctaccgtacc accaattacc tagtgtacgt tgtagccatg gaattaccca
    17581 gcgatccaac ccaatcgcac gtccggaacc gttcatcaat gcgaaagcaa caaaaatgaa
    17641 ccaaatgttt acccagtaaa acatgcctga taagcagaac gtgatcacca atacaatcgt
    17701 tgtcgcactg catagccatg tgaataa
//
LOCUS       DACCYH010000059        17396 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.060, whole genome shotgun sequence.
ACCESSION   DACCYH010000059 DACCYH010000000
VERSION     DACCYH010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.060"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(145..690)
                     /locus_tag="IVY85_001904"
     CDS             complement(145..690)
                     /locus_tag="IVY85_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8044918.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(702..1394)
                     /locus_tag="IVY85_001905"
     CDS             complement(702..1394)
                     /locus_tag="IVY85_001905"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP8044919.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1413..1697)
                     /locus_tag="IVY85_001906"
     CDS             complement(1413..1697)
                     /locus_tag="IVY85_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044920.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1710..2273)
                     /locus_tag="IVY85_001907"
     CDS             complement(1710..2273)
                     /locus_tag="IVY85_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8044921.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2501..3163
                     /locus_tag="IVY85_001908"
     CDS             2501..3163
                     /locus_tag="IVY85_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP8044922.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3287..4483
                     /locus_tag="IVY85_001909"
     CDS             3287..4483
                     /locus_tag="IVY85_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP8044923.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4584..5960)
                     /locus_tag="IVY85_001910"
     CDS             complement(4584..5960)
                     /locus_tag="IVY85_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8044924.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5997..6863)
                     /locus_tag="IVY85_001911"
     CDS             complement(5997..6863)
                     /locus_tag="IVY85_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP8044925.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6952..7977)
                     /locus_tag="IVY85_001912"
     CDS             complement(6952..7977)
                     /locus_tag="IVY85_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8044926.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7995..8669)
                     /locus_tag="IVY85_001913"
     CDS             complement(7995..8669)
                     /locus_tag="IVY85_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8044927.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8853..9746)
                     /locus_tag="IVY85_001914"
     CDS             complement(8853..9746)
                     /locus_tag="IVY85_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8044928.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9820..11517)
                     /locus_tag="IVY85_001915"
     CDS             complement(9820..11517)
                     /locus_tag="IVY85_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8044929.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11510..12823)
                     /locus_tag="IVY85_001916"
     CDS             complement(11510..12823)
                     /locus_tag="IVY85_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8044930.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12823..16860)
                     /locus_tag="IVY85_001917"
     CDS             complement(12823..16860)
                     /locus_tag="IVY85_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP8044931.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16873..17331)
                     /locus_tag="IVY85_001918"
     CDS             complement(16873..17331)
                     /locus_tag="IVY85_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8044932.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4884 a         3901 c         3025 g         5586 t
ORIGIN      
        1 gttgataata aagagattta ttaacgtaaa aagaataagt ttttttaaaa taacgtataa
       61 aaaagagcat aaaacaaagt cgtttgtttt atgctcttct tttattattt tgtgcttttt
      121 attttatcaa atcactttgt ttttttataa aatttctgcg cctagtgtgg actcaaaatg
      181 tctcaatgcc cattcatgtc caacgggatc gaaacttgcc acggaatctt tgaaaatatg
      241 gagtttgtat cctagattat acgcatcgac tgctgtatgc aacacacaga tatctgtaca
      301 tacgccggtc aaataaatat ctgtgatctg gcgttctcgc aaacggatgt ctagatctgt
      361 tccactaaaa gcggaataat gccgtttgtc tatccaataa acattttcct cttgctcata
      421 gtcttggtat aaacttttta aagagccgta caattcccgt ccacccgttc ctatcacatt
      481 atgcggagga aataagcggt tctccggatg aaaggaatct tttggatcat gcgcatcaat
      541 ggcaaaaacg acaaaatcct tttgatcaat aaacttcttt gtatgagcag tcagcttttt
      601 ttcaattact tgtcctgctg tcccagtagt cagctttcca tcctctgcaa caaaatcata
      661 tgtgtagtca atcgagatca atgcgttcat cttagtcctc cttatacgag atgtttgaca
      721 aattcgatca ccatttctgc tgatcgtttg cctgcgtctt cgataaattc gtcaaagctt
      781 tgtgtcgccg agtgatctgc tgtgtcgctc attgcacgta cgatcaaaaa tgggatattg
      841 aactgacgag ctgtttgtcc aacagcggct ccttccattt cgcaagctaa tgcttcaggg
      901 aaattcgtta agatttcttt gattttatct ggcgagtcga caaatgtatc acctgtaacg
      961 atcaatcctt cttttgcgtt caaattcgtt gcttttgctg ctttaaccat ttcactgcgc
     1021 aaatatgtgc ttgcttcata atatagaggc attcctggta attgtcctgg gttgtagcca
     1081 aatcctgttg catctgcatc aaaataagct actttgtctg atataacgat gtcacctact
     1141 tgtaagcctt caccgattcc gccagctgaa cctgtattga tcaccatgtt cacgccgtat
     1201 tgttggatca acagacttgt ggtaatagat gctaagactt ttcctatacc ggaacgtacg
     1261 acgatcactt cgtggttacc taatgaacct gaaataaata aagcgcctgc gcgttcccat
     1321 gataatggtt cgcttaggtt ctctctcaaa atttttactt cttcttccat tgcgccgata
     1381 ataccgattt tcatcttttt aattccttct ttctatataa atgcgattgc taaaaaaacc
     1441 acacataaca ttaggataac aataataaga gatttcatca aaaaagagtt ccatttggtc
     1501 gtcttttccc gttcgctgat ccttgttttc gtgattggtt tattcttttt gctttcttta
     1561 cgataaaagc tggcaatctt tttttcttct tgctgataag ctgtttctgc ttttcgctgt
     1621 ttttttaatg actcgcttgc ttgtgcttgt tgtcgcttgc gaagttcgct tcgcgtaata
     1681 agcggtcctt tggccataag ccgatccctc tactttcctt tggtcaatag ttcccagtat
     1741 tgaatcgcat aaatcgtctt tgcatcacaa atcaactgat ctttcatcgc ttgttgcgcc
     1801 tcttcaagag ttagatgata caactccagt acttcgtctt catcttgtgc taaaggattc
     1861 tcgaccttct ctaccccttg tgcatgataa atatgaagga cttcatttgc aaaacctggt
     1921 gaaagataca tcgaagtaag atggctcagc gacttcgcac gatagccagt ttcttcttct
     1981 aattctctag ctgctgtcat ttcgggattt tgtccttctc ctggatcgat ttttccagct
     2041 ggaatctcca aaatcacttt ttctaaaggt tttcgaaatt gtttgacaag aagcagacga
     2101 tcttgttcgt caaaagcaat gatcccgaca ccgccaggat gaaataccag ttctctcttc
     2161 gaagttcctc catctggcaa acgaacttcg tctacagcaa catcaataat ttttccgcta
     2221 tagatttctt tgcgactgat tgttttttct tcaaattgct cattgttcag catatcttct
     2281 cctgctttct ttgtaatcat aactgctttt ttttcatttt tcaatttttc tcaaaaactc
     2341 cttattagtt taccatagat aaaccttaga ttttctctaa atatagaaaa tccttcttta
     2401 gtccgatact ctttctagta gtccgtatgc taaaataagt atgtaatcga ataacgaaaa
     2461 gaattcaata ctcaacgata aatgacatgg agggtttatt atgataaaac gaaattggcc
     2521 gtggcttcta gcattggtct tgctatttct ctttgtccga aacaatagtt tttcttttgt
     2581 tctaggactt ttattgatag gtgtagtttt agttttgtgg ggactctttg gttcgcgcag
     2641 gaaaaaggga aaacaagaag aaattcctgc actgactgac gaacttgaaa ctcactatga
     2701 aaaaagcggt atgacagcga atgaaatttc attttttcga caaacaatga atcaaacaaa
     2761 aaatgagatc aatcaattgc aaatgaatat gcagcagaca gccaaactta aagcaatcga
     2821 tctccgccat gatccagtca aagcagcgaa agctttattc aaagaattgg tgaaagaacc
     2881 aaaccgactg catgaagcaa gtcagtttct ttatacgcat ctgccgaatc tagttgattt
     2941 gacaaataag tataatgaga tcaatgacca tgagatcaaa aacaaacaga cctatgaaaa
     3001 actagaagaa agcgcgcaga tcatcgacca gctgtcagct ttaatcgcac aagattatca
     3061 aaaattcgtt gctgaggatc tagatgatat cgacgtagag atatctgttg cgaaacagag
     3121 cttaaagcgt gataataaat atgaaccaga acaaaaggat taactctttt gttcataaaa
     3181 gaagactcgg aacattcaga tgatgtctgg ctgtttcgag tactttttct aaagtagaga
     3241 cattcatgta agaatcgaga acagattcaa caaggaggaa ttttttatgg atcataaacc
     3301 gactgaaaca aaagatgtta caccagttaa cgatacactt gaagatttat tgaacaaccc
     3361 gttttcaact ccaacagata cgctgaccac ttcacaacaa gcggagattg atcagctcca
     3421 agagcaacag acagctgctc gtttaattga caaactaccg gcggaacgcc aagaacaagc
     3481 taaacagcta gcagcaaaaa tcgatgctaa tgatgcacag tctgtcatca gctacggatc
     3541 ggctgcccaa gcaaaattaa gtgaattctc acaatcgatg ttgaatcatg tgcaagcaca
     3601 agatattggt cctgtaggag attccttaac tgaactgatg tatcgtctcc aagaagccaa
     3661 tcctgacgaa ctgcgtgccg gagaagggaa catcttccag cgtgtcttcg ggaaggtcaa
     3721 acaatcgatc tatgaagtga cagcaaaata tcaaaagatt ggtgcacaaa ttgacaagat
     3781 ttcagtgaaa ttagataaag aaaaagacgg tctgctaaaa gataatctga tgttagaaca
     3841 gctttaccag aaaaacaaag actactttga tgcactgaat atttatatcg ctgctggaga
     3901 attgaaaatg gaagaacttc aaacaacgat cattccagaa gcgatgaaaa gagcagaaga
     3961 aactggcgat cagatggatg ttcagattgc caatgattac acgcaattcc tagatcgtct
     4021 ggataaacgt acgcatgatc ttcgcttagc tagacagatc acgatccagc aagcaccaca
     4081 aatccgtttg atccaaaaca caaatcaggc cttggctgaa aagatccaag catctatcgc
     4141 tacagccatt ccgctgtgga aaaaccaagt cgtgatcgca ttgaccttgc ttcgtcaaaa
     4201 agatgcagta actgctcaac gccaagtctc tgaaacaaca aacgacttat tgaagaaaaa
     4261 ttcagaaatg ctgaaaatct cagccatcga aacagcgaaa gaaaatgaac gtggaatcgt
     4321 ggatatcgag acccttcaaa ccactcaaaa tgatttgatc gaaacaatcc aagagacatt
     4381 gcgtatccaa aaagaaggaa aagaaaaacg tcgtcatgca gaagtagagc ttggacacat
     4441 ggaagaagat ctgaaacaaa aactgttgga tttaacccaa tagatcacct ttcgcttgtt
     4501 actaaaacaa acgcccaaca acagtttatt acaactgttg atgggcgttt ttcttatctt
     4561 tcttctaact tctccattgg agtctatttc tcattcatct tgctgtgtcc atagccatag
     4621 ccaaaataaa tgatcagtcc aataaccatt acgataccga acacgatcca tgtcacagtt
     4681 tgcagacgaa gcatcaatac aatacaagtc aaaatggaga cgattggcaa tagcggaacg
     4741 aatggcactt tgaattctcc agcttttggt ttgccaaaca tttttctcaa cttcagtaag
     4801 cctagactaa caagcatcaa ataggcgagc gccatgatat tcgttagttc cgctaataca
     4861 ttcaatggga aaaatccggc aaagaataca gctgccacac cagcggcata agtcgctttt
     4921 ttcggtgtac gatgcttttg atcgatttct gtcataaact taggaagtaa gccatccttg
     4981 ctgacagcaa acagtaaacg agcaagtgaa tacatcatcg agatcgttac cgtcagtagc
     5041 gttaaaattg cccctacagc gatcaccgcg cccactgttc catgtcctac atacctcatt
     5101 gcaaaagcca ctgggtcatt gactcctagt tcagtatacg gaacgattcc tgtcaagatc
     5161 agcgtcacga tgatgtaaag gacagtagca atcagtatcg aaccaatgat tcctcttggc
     5221 acatcttttt gcgggttttt gacttcctcg gctgccattg caacggcatc aaatcccaag
     5281 aaagcaaaaa acacaagagc tgcaccatct agcacaccgg aaaaaccaaa aggcatgaat
     5341 ggctgccagt tatcaggttt aacgaaaaat attcctacaa tgataaacaa cgcgatgata
     5401 ccaaatttga catataccat ggcattattc aaacgtaatg ctttcttggc ttcaagcgac
     5461 acgatgtaag tgacgaatat cactacgagc accgcaatca agtcaatgta tgtaccattt
     5521 tccggattgt acccgccagt taatgcttgt ggaagttcgg cccctaaact tttcaaaaaa
     5581 ccttgcatat agccagacca tccggaagca acagaggaaa ccgctagtaa aaattccccg
     5641 atcaacaacc accctgtcag ccaggcagca aattcaccaa atacaacata cagataggca
     5701 tatggcccac caatcactgg cactcgcgaa gcaaattctg cgaaacttag tccagacaaa
     5761 atgacaacga ttgcagctaa gacaaatgaa agtgacagtg ctggaccagc attttgattg
     5821 gccgcaaccc ctgtcaccac aaatattccc gtaccgatga tcgcaccaat tccaagcatg
     5881 atcaaatcag acgtttttag ttcttttttc atctcactcg tatgattgag attgatctct
     5941 ttcttacgaa acaaatccat tgaagttcct ccccatttta ggttggaaaa cagcgattaa
     6001 actgttttca tataccctaa aaggatacca cctaaaatga ctaaaaggca accaacaata
     6061 acataaatga gctctttttt cgtttttctt tcgccaagga tgaaaatccc acctaaagta
     6121 gagataatga tccccatttg tgaaagtgag aagccaacag ctaaaccaag tgatttgact
     6181 gttagaagca tgcagatgtt tcctaagccc cataataatc cacagatcat gtttttccac
     6241 acaaactgat cgactttaac tttacggaaa gcaaagaagc ttgcgcctaa gatcattccg
     6301 atactttgag gtaagataat tgacatcgca tccaaattcg cccagttgac gatgatggta
     6361 tatacaccat aaccaatcgt agagaagatc aatgctctaa aacccttacc aaaatcaagc
     6421 attttattat ctgtatctat ttttccttcg tcatcttgtc gggcagtcaa atacgcaccg
     6481 cttacaagaa gagctaaagc aataataccg tatacataat ctttcgtttg tgtccattca
     6541 tggaagaaaa cggctcccgc aatcgtattc aaaattaatt gaaaaccagt agacaatggt
     6601 agccctacag aaacacccat gtacttcatt ccatggaact gtccattttg tccaacactc
     6661 caagctaagc cggataaaaa tccgatcaag aaaatccaag gggtgattac aggactaaca
     6721 acgaaaaaga caactaaaga aaacagaaat gcaccgatcg tcattcctag tgtttgttga
     6781 ttggcatcgc caccaatttt accacttact aaaccaatac taccccatgc caacataggg
     6841 actaatgcta ttaaaatttc cataaccaat ctccttttat ttcacatgat aagaaactta
     6901 tcagcgaaaa cgttaacaac acaagcactt tttttcgttt ctaacgattt tctaacgttt
     6961 ttcattataa aaatttaaga ttgggaaaaa taaagtcatt gttgtccctt ttccttcagt
     7021 tgattcagcc gtcaaatgga tacctaattt ttcggccaga ttttttgcta aataaagtcc
     7081 taatcctgtt gaatgtgttt tgctaagtcg accatttctc ccagtaaatc ccttatcaaa
     7141 gatccgtttc aagtcttccg ctgggatacc gatccctgaa tctttaactg aaaggctgac
     7201 tccttgcgct tccttttcta tgctcacaat gatgtttccg tgatccggcg tatatttgat
     7261 ggcattacta agcaattggt tgaagataaa agcgatccat ttagcatcgg tcaaaacagt
     7321 atgatcctca ccaacgatct cgtagcgaat atttttttgg ataaaataat ttgcctgact
     7381 gcgtatcaca ggctgaatga ttccttttag tccatattct tggatgaggt agtcccgcga
     7441 gaaactgtcg agtcttgcat aatacaaaac ttgttctacg tattcatcga tttttgataa
     7501 ttcattttct agtaatgtat acttttgatc gtcaatatcg aactcgatcg aacgaactag
     7561 caatttagaa gcggaaagtg gcactttgat ttcatggacc catgagtcaa tataatcttt
     7621 ttggtcttgt tgattactga ttgctgattg cattacttct tggtgttcac ggatcagctg
     7681 attgatatat tcttctacca gtttttcttc ttctgtcctc ccaccactca aataattttg
     7741 caaaggggaa gttttttgta tgtcggctaa ttttccccac catcttctct tgctcaagta
     7801 accacccagc agataaaaaa tcagaaagac gccctcaatc aaagctaaat aagcaattgt
     7861 gctcaaatca actttgatgt ttggcgccag ccacatcaca aagatcgtca atgcaataaa
     7921 aaacagccat cccaagatca atgtccactg atctttcaaa tatttaaaaa aattcatctt
     7981 aaccgctcat cctttcaggg aacgatatat ccctgtccta cttttgtttc gatatatcct
     8041 tggatacctg cctgttcgat ttttcggcgt aaccgattga tgttgactgt caatgtattg
     8101 tcatctacaa atcgttcgtc atcccacagt gctcgcagca atttttcccg acttaagatc
     8161 tttccattgt gcttcatcaa aataaataat agacgatatt catttttgct caaatcaatg
     8221 atctctccgt tgatttccat acttccgttg tcaacattca acgtaatgcc attgtggctc
     8281 atcatttcac tagataattc tgtgtagttg taagaacggc gaagtaatgc attgattttt
     8341 gcaatcagta catcgatttg gaaatgtttt gtcacaaaat catctgcccc catgttcata
     8401 gccatgatca tatccatatt cgtattcctg ctggagataa aaatgatcgg tactttcgat
     8461 acttcacgta ttttttgaca ccaataatag ccatccaaaa cgggaagatt aatatctagt
     8521 aatacaagct gtggttcttc tctttcaaag tctgagaaca cttgattgaa atcggtcgtt
     8581 ccaaaggttt caaattgcca tttctcaagt tcctccatga tcaattgtcg aatgatctct
     8641 tcgtcttcga ctaccatgat ttttgccata aaaaatccct ccatctttct tcttgctact
     8701 acaatatcaa agaagccctt gtgaaacaag ttggcttacc tggaaaatgt cattttagca
     8761 aacgttaaaa aaaactggaa atatgcgatt taatcgcgtt tgtctcttcc aagcagctta
     8821 aatttattat gttattctta ataattggtc tattatctgt tatttttctt tcgttgtttt
     8881 tgtcttttaa attttcgata acaccaataa ccgaaaagcg ttagcactag caatatacct
     8941 agaaggagta taagtaatcg ataaagatgg tatttttttg tctgttctat ctttgaagag
     9001 acttcttctg taaagggaac tcgtcttcct gtcactagta atcggtgagt gttcacggtg
     9061 taaggtgtac aagtgataag tgtgactaga tcctgctttt ctctaattgc taatgtattt
     9121 gtttttgttg gaaggacaac aattttctca acgacttcat aagcgagcat ccgaccatac
     9181 acttcaagat agaatcggtc atcttgctcc atttcttcaa gatccgtaaa gagttttttc
     9241 tcagcaatac cgctatgggc ggtaatcacg gaatgtgtac tctctcctcc agttggaaaa
     9301 gaggtgcctt gcaggactgt cgctcctcta tctaacaaaa gatcattcgt ttcatcaaat
     9361 aaaggcaagc taacattgat tttcggaata tagatggctc cgatcatatg ttgttcgtaa
     9421 tattcttttc ctggattttg gacatttcta accgcttgct caaaaggatc tttgacttgt
     9481 cccattcctg ggattactga tgtatgagca agcgtctgat ttttcttttc atgcactgct
     9541 aattgttgct catgtttttc ttctttatcg cgttcaagct gcttttgata attctcgatc
     9601 cttctttgat ccaaataatt gctgagagca tctgctaaaa atggatatgt aaaaactgca
     9661 gctccggcaa gaaacatcac tgccataaat aattgtaata aaagactgat ttgggtttga
     9721 ggtctattac ctattttctt tttcattttc ttcaccttct tatttaaaaa aactgcagca
     9781 acctttataa aaccgattgc tgcagcttat ttttgttgct tatacttcag acttcatttt
     9841 agatttacgg taccagctgt acgctccaat catcaaactt attccgatta gtaagaatgc
     9901 caagagcccg ttcccaccag tagcaggaag tgacccgcgt ttcatattcg gtactctttc
     9961 tggccctgga ttttctactc ctacgatctg aatttcttca ttatagcttc cataagagat
    10021 gataaattca gtaaaggctg cctcacctgt tggaagtaca taaccttctg gcgccatggt
    10081 ctctttcatt tgataggttc cataatctaa cccttgaacc tcaaagtatc catttccatc
    10141 tgcgacaaat tctgttgctt gtccgcttgt tgcccactca attctatctg ctcctgattc
    10201 ataaacagct aaaggcgact cccctttata aaatattgcg tattcagaac ggtcttcatt
    10261 ccacaattcg aatcttgcat ctttaagcaa ttcactgctt gatgcatcat atttttcaaa
    10321 ctttcttcca cctgttgtga ctttctctgg cggctctact ccttcttcat aatcaaattc
    10381 atcatttcgt ccgatttcaa taaccgctgt gttgttatgg aaatcatccg cttctagttc
    10441 tgtggaaacc ttcaaattat acgtgatcgt taaatctcct ccacgtgcat ctgctaatct
    10501 acctaaagta tctactttgg ctactccatt ttcttctgtc aaaatcgtca cgacaaaccc
    10561 gttgcttact acttctacta tataatcttg tccttttgtt aaaatcgtct cgccggctcg
    10621 cacttctatt tcctgatcat aataagttag accaactgta ggcgtatctg tgattttgta
    10681 atttttgata acagcgcttc cgttttcaag gacagaatcg atgaaataag gaataggtgc
    10741 tgtgatcgtg taagaagccg tttttcctct ttctagatca atataagaaa tcgtttcttg
    10801 ttctccagct tcattaataa tcgttacttg tctaaggtcg ctttcctcta cattcatctg
    10861 tttatctgct tctctgattt cgtttttcgg atacaaatgc acatctgtat tgtatgtgtc
    10921 tttccatgta gaaccatccc acacgccatc caccatatca ggcatcatca ctggcatcat
    10981 aacaaccatt ggagacgcga ttctggaaat atgagctggc gaataagttt ctaagaaaag
    11041 atagacttta tctctagaag aaggtgcttc ttccgtcact tggacggtag agaataccgc
    11101 ttctccagct tcattcgttg tttgtttgtc aatacttgtc agtcgatatt ttaaaaacca
    11161 actttctgtg tgccgttttt gaatccattc aatcgcttcg gcagaattaa gaccgtcttc
    11221 tcgttccggt gttaaaggat tatctcgaat caaatcgtaa tatacttccg ttacgtcaaa
    11281 caaatcaaaa tcgactcctg gaatattttc ttcaccaaaa tccgtagaaa ccaatccgct
    11341 attcggtcgt tcttccggaa cagatgagaa accttttttg tgtaatgtaa tcgtcacttc
    11401 acttggtttt tgtgcgactt cttcggcttt gactagtttt ccgttcccga aaatccctat
    11461 tcccgcagta acgagtaaca gtacactcat tcccgtgaca atcatttttt tattcatttt
    11521 tttctccccg ttcttttttt attaataaaa cagcacctaa caataaaaga attcctagta
    11581 aagaaatcaa cgttcttgct tcattcgttt gcgggaatac ggaccatttt cctgattgac
    11641 ctggattgct tggaccactc ggctctttcg gtttgtccgt tgtttctttt acggtctcgt
    11701 tataaacacg tggtgttgcg gattcatagg cagagactgg aactttcccg gattctaatt
    11761 cagccatctc ctgtccgtta actactacat attgtggttg gccttttgca tccacccaaa
    11821 attcagggat gatgacctcg attctcttac ttgcttcatc catctcgtaa ccttccacac
    11881 cttttaattc ttcaaaataa taggtgccag aaggcaagaa acgttgtcct gtcgtaacaa
    11941 ggccctcttt atccgaaatg aatttgctta catttttgtc attcaaagga tcattgtctg
    12001 ctggtttgat ccattgattt ttcaagtcat tagcaggagc catatctaaa taatatttcc
    12061 ccccttctat catctgatat agagcaaaaa cagcaccttc taatggtttt cctttttcag
    12121 ttgttcctct ttgacgtcca tacttgaaga agtatggatc tcttagataa ccgacatttt
    12181 taggatagat atgaatctct tgcagttcct cggttgattc tgtcgggtga taaattggca
    12241 aaatagcagc aattgggata gctgtctgat cgatatcaac atccaatcca acttctggat
    12301 caatcgattc ttcgaaaatc aaataaactg catcccgatc ttggtttttc ttaggtgctt
    12361 tgatacgtgc aatcccgtct cctaatccgc caaccgtatc tttcccagtt gttaccgaag
    12421 tccctatttg gacaagcctt tcttctgtga ttagctgacg gatattgttt ctgttcgttt
    12481 cagcgatttg ctggacgaat tctttaggag tcatgttttc agcagctttt ttttcatgat
    12541 aataatcggt catgtcgtaa atggcaaatg tcgctccatt tagtggcacc gtctgtgaaa
    12601 tgatatctgc tgtttcccct gcttcctcag agatagccaa gccatcattt tcatggtatt
    12661 caaattgatc gttataatcg atatctcgaa tcatccgttt atgcaacacg aattccacga
    12721 catcttcccc tgattcttct gccgacatcg catgtggaag aaggactgct gcaagcggta
    12781 gtaaaacaag taattgaagc atcaaacaca acagcttttt cattaccttg ctccctcctt
    12841 tttagattgt ctgacaaaca aaccataggc tgctcctgca agcgccatga agaatgcagc
    12901 aagcaggata tagaataatc gtccggatcc tcctgtagca ggcaacggat tttcaggatc
    12961 gttcccgaca ctaagctgta atctgattcg atttgtctcc aaaagttcat attctaactc
    13021 ttgctgtaga tcaggcgtct ctgcttcttc gtttggataa tacgaaatct caacttttag
    13081 tgtacccgct tcttttcctg gaactacccg atcattttct atgtcaggtt cttctcgata
    13141 actaatttct aaaataaagt aacctggaag ttttctatag ctgtcaggtg acttcacttc
    13201 cttgatccga tagacccctt ccaacaagtc tgaagcattg aatagggaag aatcgtttgg
    13261 atctggtgcc aagcctccct cgatgatttc atactctcct gcactatttt gtttctcaat
    13321 tgaaaattct gcgccttcaa gacgtcggct attcatactg tcaattttca aaagttccaa
    13381 atcgattggc gctagttcat cttctaaggt tacttgtagt acaccatcga tgatttcata
    13441 gccttccggc aagggagcag tttcgtcttc gatgaccgta tcttcaatga taaacttacc
    13501 ttcttcattc agtgcaaact ggaacacttg atcagatagc cgatacccat ttggtgcgac
    13561 agattccgag acctcatatt caccaataga cagatgcaga ggttggtcgt aatttaaatc
    13621 gacatacttt tctactccta cggcacgaga atagaagtat ccgatcccag gcaaacgttt
    13681 cgattcttct ggttctaacg gattaccagt gtatgctcga tacagcggga aggaagaatc
    13741 ccccactttc ctgatatcga aacctgcacc atctaatgta tggccttcat ggtctaattt
    13801 tctaactcgg atctcccgat aatcaaacgt attgtaagca gtgaatgcta cccataaacg
    13861 atcctcttca tctgttgtat acgtgagata ttgccaattt tcttcacctg tttgcttgag
    13921 ttggatagtc cctatagcat tatcgtaaag tcgcaagatc catgtgtcat tttcatcagg
    13981 ttctttgaaa ccttctgggt tctggctctc aatgattttg tattctgcat agttggtagt
    14041 ttcttcacta tccttataaa caaatgaatc tggctctccg attggatgaa taactttctc
    14101 accatccacg attacatatt tcccgtcttc atctactgaa tagaacgtga gtctcccttt
    14161 ctcttcctct acttcgttca caccagaaag ccctttcgct actggatatc cgatttccag
    14221 tccgttacca tctgtttcat aaagttcaaa ctctgcgcca ttgattggca tattactgcc
    14281 ttcaattagt ttattgatat tcatgtggat tggcttgaat tcatttcgca tttccagttt
    14341 gacatacaac tcatcatact ctgtgccttc tatgttaggc tcttgtacaa cagaaagctt
    14401 gtgatattct ccttcataat aggaattcgg atctttacct tccggtccgc cgactctcga
    14461 accagatgta tattcatcta caagctcgta gtgacctaac atctcatctt cgttggctgc
    14521 atattttttg atttggaatt gatacgtctc atctactaac tgataacgtg gtggtccagc
    14581 gatctctctg atccaaaata tgtcgctgac tgctccagtt tcatctccta aaatcggcat
    14641 cttcgtttca aagatcgcta ttccgtccgt ggtagtttta cttgtttcga ttggttcggc
    14701 ttccccactc tctggatttt ggtcatataa tgcaaattcg actccagcca acggctcgcc
    14761 attttgaccc ttcttgctga cttggaaatt acggccttta tgttcgtaac gaatggaaga
    14821 agatgggaaa tacatccgtc cagtagtgat atcattctca taatcctcca ggttctgaat
    14881 ataggccata ttgttcactg tgcaaaaatt tccgtcttga gctcccgcat caagttttac
    14941 ttggtacttg atcctgacca tttccccttc acctaaagaa atcggagaaa atttataagc
    15001 ttggttttcc cgtccgacat acgttgtcgg agtcgccact acttctggtg gggcttgtcc
    15061 atgacgttct tcatcccata tttctggctc ttggtacttc atgccgtttt ctcctggatc
    15121 tagatggtaa aactgaatat ctgccgatct gccagctcca aatggtgaga cattaaaacc
    15181 agtttcatca taaagtgtgg cttgctggat cgttctcttg aaggtagaag agatatcctc
    15241 caaggctgta acaatgtcat tcttgttgtt cgcattgtaa tatcgcggtg ttacgccatc
    15301 ttcttctaaa tcagcaatat ttttcaatac atttcgtcca atagcatcta gtcgttgtct
    15361 cccgctaacg tttccatcga ttcctaaacc aatggagaag atacttggcg ggttctcttg
    15421 atacttgttt ttcagccaat gatgataagc aactgtgttg atcgcagatg acttttcttg
    15481 cgaagtagga ctgccattcg tattgttatt agaagaacca tcaccaaccc agtgagtcgc
    15541 tttattatct ccttctttta cctctccata acgatagtgt acatttactt catcttctgg
    15601 acggttaaag tcagatgcat aggtaactgg atgagtaaac cctgcattag aagtattccc
    15661 agaaaactcc tcaaaatttc tgaacaaaac gtcattatct tcagcaatct tatcttccat
    15721 cacagtccag ttgctccaag ctccattagt gctcgtacga taacttgact gaatcggtgc
    15781 ataactgaag gtcggtgtcc catctcccac aacgattagg attttttctg gatttctatt
    15841 ttctccgcca ttgtctttat acaatgtttc atagccattt ttaagcccta gtgtaagcgg
    15901 agttcctccg attggtgtac gatttaatgc tgtattctca cttcccgtca aacgattgat
    15961 atcatttgtc aatggaaaga tgtccgaact gatttgctca tggttattga tagctgtact
    16021 attatggtaa aaattcacca taccgattcg aatatccaga ctcgggtcag agagcagatc
    16081 tttactcatt tcgtttactg cctcaatcaa cgcagcattt ttagtttgac tattggtccc
    16141 agcggttaat tcactcatac tcgctgactt atctagaaca aaaacgatat cgattggact
    16201 gatttccttt ttctcgcccc cgatgacatc tagatagaca ttgtaaaggg ttgttgcatc
    16261 ttcaggatgt agatcagatg ctgtgctgct tggcagaaca ggttctaccc attttttgat
    16321 gtacgccccg ttataatcca agtaactgtt ttcaaaattc cgttcatttc cccaaagctg
    16381 aacggtttga gctggaacgt ttctatcttc ttcgcctgca gttgatccat tattagtagc
    16441 atagtcgtaa tttcgccagt tcgctccccc gtttgtataa tcactatcat cgatcaagtt
    16501 ctctccgtca tatggcgagc ctttgtctcc ttgatagcgt tcattgcttt gtgtccaatt
    16561 tcctaacact tttgtatagt cattcgttgt aaacttcggg tagcgatgct gttcgttttc
    16621 aacttctccg ctaggataga tatattcata ggggtcttgc gtagctgttg gcccaagacg
    16681 ctccaaaata ccgttgaata actggcgttg aatgcgctgg agtgcttgac cgctgggtac
    16741 gattggttcg tccagctttt ccatctcatt ttcttcgcta cgttgaattt cagcggaatc
    16801 ttctgtatca gtttcatttt cttgtaattc ttggttatct attctttcat caaccaccac
    16861 ttgaagaggt agttaccctt cgttcacatc ggggatcttt tctgtttcaa aaatcaatgt
    16921 gccggtttct gcttcttttg aataaacttt tccgacatac cattcttgac catcgatcgt
    16981 ctcgactttt aggtcttctc catcttcatc ataggaggat agacctgttc cacgaacgtt
    17041 ttttacggta ccaatcccgg cggctgccgc atcgattgct aaacgtattc taccttcatt
    17101 ggcactttcc tttttttcaa aagccagatg ccattcaagg gtttcttctt tttcaatata
    17161 cttcccttca atttttgcat agtcctcatc taagaatacc tcatcctcct ttgtttctgt
    17221 tgactcagca gctacatcca gggtcgtcat atgatagact gactggaaat atggtccaaa
    17281 tagcaaactg acagttacta actgcaaaag cttcaatttt aaccctttca catccttata
    17341 ctcctttcaa aaatatttgt cttctttcac aacaatcaac aaaacaatgc ccatct
//
LOCUS       DACCYH010000060        16937 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.061, whole genome shotgun sequence.
ACCESSION   DACCYH010000060 DACCYH010000000
VERSION     DACCYH010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.061"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..293)
                     /locus_tag="IVY85_001919"
     CDS             complement(<1..293)
                     /locus_tag="IVY85_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8044933.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYG"
     gene            complement(295..492)
                     /locus_tag="IVY85_001920"
     CDS             complement(295..492)
                     /locus_tag="IVY85_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP8044934.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(886..2613)
                     /locus_tag="IVY85_001921"
     CDS             complement(886..2613)
                     /locus_tag="IVY85_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8044935.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2606..3799)
                     /locus_tag="IVY85_001922"
     CDS             complement(2606..3799)
                     /locus_tag="IVY85_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8044936.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3986..4630)
                     /locus_tag="IVY85_001923"
     CDS             complement(3986..4630)
                     /locus_tag="IVY85_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8044937.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4724..4858)
                     /locus_tag="IVY85_001924"
     CDS             complement(4724..4858)
                     /locus_tag="IVY85_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP8044938.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4860..6992)
                     /gene="feoB"
                     /locus_tag="IVY85_001925"
     CDS             complement(4860..6992)
                     /gene="feoB"
                     /locus_tag="IVY85_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP8044939.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6989..7462)
                     /locus_tag="IVY85_001926"
     CDS             complement(6989..7462)
                     /locus_tag="IVY85_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP8044940.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7863..8087
                     /gene="nrdH"
                     /locus_tag="IVY85_001927"
     CDS             7863..8087
                     /gene="nrdH"
                     /locus_tag="IVY85_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP8044941.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8246..10405
                     /gene="nrdE"
                     /locus_tag="IVY85_001928"
     CDS             8246..10405
                     /gene="nrdE"
                     /locus_tag="IVY85_001928"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP8044942.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10455..11420
                     /gene="nrdF"
                     /locus_tag="IVY85_001929"
     CDS             10455..11420
                     /gene="nrdF"
                     /locus_tag="IVY85_001929"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP8044943.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11509..12648)
                     /locus_tag="IVY85_001930"
     CDS             complement(11509..12648)
                     /locus_tag="IVY85_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8044944.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12957..13670)
                     /locus_tag="IVY85_001931"
     CDS             complement(12957..13670)
                     /locus_tag="IVY85_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP8044945.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13924..14625)
                     /gene="nagB"
                     /locus_tag="IVY85_001932"
     CDS             complement(13924..14625)
                     /gene="nagB"
                     /locus_tag="IVY85_001932"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP8044946.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14859..16391
                     /locus_tag="IVY85_001933"
     CDS             14859..16391
                     /locus_tag="IVY85_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8044947.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16582..16761)
                     /locus_tag="IVY85_001934"
                     /pseudo
     CDS             complement(16582..16761)
                     /locus_tag="IVY85_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(16781..>16937)
                     /locus_tag="IVY85_001935"
     CDS             complement(16781..>16937)
                     /locus_tag="IVY85_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP8044948.1"
                     /translation="SSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGT
                     LRYSLGY"
BASE COUNT         5261 a         3486 c         2914 g         5276 t
ORIGIN      
        1 ccataatctg ctcgatataa accatttatt ccagcagttc ttccaatttc actcaaattt
       61 cttattaatt cctcttgata ttcagtgaat cttattttat tagtataagc agtaaaatat
      121 agataattca ccaatctatt tattaaagga ctgggatcta attcatattt ttgtttttct
      181 aattttaaat ataccttttg taaaacctca agaatatcag aatatttagg atctttactt
      241 atagatatat cattatataa ttcatgaaca atttgcttag catttttttt cattttagca
      301 cttccctgga attgctccac ctaaaaaacc acctatagac attcctgcaa tacaagtagt
      361 tgccttggcc caatcgaccg tacatttatt tttagtgcaa tacactccat ttccataata
      421 ttttccacta tgagtggtac ccccatagat aagttgtgtc tctttaatag acaaaatttt
      481 taaatgtttc ataatctaat cacccctaaa ttaatgtttt tagactatta ttctaacatt
      541 cattaaattt caaaaaacaa ttccttcttg aaacgtagca aaataattcc tgaataagaa
      601 tttaatcact tttaagtata taaactatgt atattcttta atattttttg agaatgcact
      661 tcgtacataa taaactttaa tcacattagt ctttcatacc tacgatcgat ctctttgact
      721 cgcacccata aattcataat agtttgtcgg cgtctttgaa cgtttcgaag atgagctacg
      781 cattctcttt ttttatagac aaaaaagata gagacgaaca tggagcttcc aaatttggat
      841 atttgatcca attttggaag ctccatttat ttcgtgtctg tctttttatt ttttccagct
      901 ttcgactaat tcttggttct ctttgatcca gtcttttgct gcttgctgtg gatcttttcc
      961 attgttgatt tccaacataa ctttttccat atctttttct gaccaatgga aattgtctaa
     1021 tactttgtaa gcttctggct ggtcttcttt caatcctttt cgaaccatcg tatggatctg
     1081 ctcttcgctt cccattccgt tttcaggatc ttctaagtat ttcagatcat atttggcaaa
     1141 catccaatga ggtgtccatc ctgtaactac aattggttcg tgttttttga tcgcttgacc
     1201 tagtgcaacg gtcattgctc ctgaagaaga tgtctctact ttccagtctt tcaagttgtc
     1261 atatttttgg atcgtgtttt cagcagccgt aactacacca gctcctggct cgattcctat
     1321 gattttctta cctgcctgat cggttaactc atcgatcgaa tttacatcca tgtaggctgg
     1381 cactgctaaa ccaacttttg ctcctgtcaa attggcacct aggtcttcga cttgatcttt
     1441 atattgtgca tattgtgaac catgagtttt aggtagccaa gctgaaacca tggcatctga
     1501 ctcgccattt gaaacagatt tccacatgat tgaattatct aatggcgtca tcgttacatc
     1561 atagcccatt tgtttcaata cttcaccgac tacatttgtt gaagcaactt ctgtatccca
     1621 ttcaacataa gataagttga tacgttttgt ttctttggca gatgaaaaac tccctatacc
     1681 aatcgcacct aagatcacta ctactacagc accaatgatc agtccttgac gttttttact
     1741 tttctgtgtt gttgctcctg cagctttttt attgtttggc tggttcaaat gctgtgtaaa
     1801 acggtcaatg atgattgcta agataactaa tgcaaccccg ttgacaaatc cattcccgac
     1861 ttgtgctctt tgcaatgctg ataatacgcc gcgtcctaag ccgggtgcac cgatcataga
     1921 ggcaataacg accattgaaa gtgacagcat tgttgtttga ttgatccctg ccatgatcgt
     1981 acttttagct aaaggcaatt ctagtttgaa tagtttttga cgacttgtgc tcccgaatga
     2041 atcagaagct tctactaatt ctttaggaac ttgtcgaatc cctaaattcg taaaacgaac
     2101 ggttggtggc agagcaaaga tgactgaggc aaatacccct ggaaccatcc cgataccgaa
     2161 aaatgcaact gctggaatca aataaacgaa tcctggcatt gtctgcataa agtcaaggat
     2221 cggtttgata atactttggg ctttactgct ttttgccatc aagatcccta atggtacccc
     2281 aatgatgatc gagatcacac tagataaaag aacaagtgtg acagtattca tcaaatcatt
     2341 ccatagacct tggttataaa taaaccatag accgattaat gtgaacaacg taagtcctgg
     2401 acgtttctta gaaataaaga aagctgctac cgtcaataac aagataaaca aaggagctgg
     2461 aatcactaac agtaaatttg taatccccga catcactgcc tgtccaatcg tttgtaaaaa
     2521 tgaaaatagg ccagaaaaag ttgtcgtaaa ccattctgtg atattttcta cccagtttgc
     2581 cactggtaat tgatagttca atagattatt catgttcact cacctcgctt gtttctgcta
     2641 atgcttcaat cacactacct cggatgacga caccttttaa ttttccatcc tctacaactg
     2701 caagtggagt cggtgagtca taaataatat tgaaaatatc acttaccacc atgtctttcc
     2761 caatcaccgt cacattttca tctaggaagt cgatcaatgg acgtttttgc ttcctagctt
     2821 ctaacgcttg atctgcagtg atgatccctt tcaggtgtct ctttctgtca acagccatca
     2881 gcatgctgac ttcttcatta cgcatccttg tcaatgcaac gtttggtcca tcagattcga
     2941 tattcgtcgt caatgcaggt accatgatgt tttgagccgt aagcactttg gaacgatcga
     3001 cttcttccac aaattcacgt acataatcat ttgctggatt ggtcaatatt tcttcacctg
     3061 ttccgatctg catgatttcg ccgtctttca tcaaggcgat tctgtcccca atccgtaaag
     3121 cttcattcaa atcatgggta atgaaaataa tcgttttttg aacattggct tgcaggtcca
     3181 gtaattcgtc ttgcatttct cgccggatca gcggatcaag agccgagaac gcttcgtcca
     3241 tcagcaagat ctcaggatca tttgctaaag ctctggctag gccaacccgt tgttgcatcc
     3301 caccagataa ctgactagga tattggtctt taaatgaaag caaactagaa ttttctaatg
     3361 ctttttctgc tttttcttgt cgttcttctt taggtacacc acggacttct aacccgtatt
     3421 ctgtattttc caagatcgtt cgatgtggaa acagaccgaa gttttggaaa accatattga
     3481 ttttatgacg acggacttct cgcaattctt ctttgcttaa cttcgataca tcttctccat
     3541 caatatagat actgcccgaa gttggctcaa tcagacgatt caatagacgg atcaatgtgg
     3601 atttcccact cccggaaagc cccatgatca cgaagatttc tccttctttt acgtcaaaat
     3661 tcacgtcata cacaccaacc gttgcaccgg ttttctttaa aatctccgtt ttcggctggt
     3721 gatcattcat catatctaaa gcggcttgtg tctttttacc aaatatcttt gttaaatgtt
     3781 ttacttctac ttttaccaaa caaattctcc ttttttatgt aaattcattt ttctttctga
     3841 aaaaagtgac cacactatgt taacatcatg tagtcacttt gtcaaaaatg aaagaaattt
     3901 gcttgtccaa actgaaaaaa acttggctaa atctttgtaa ataaaaggtt tagccaagtt
     3961 aatatttttt taatatttca cacaatcatt ctgcaagctg atgctccggg taaaaaaagt
     4021 ttgtcatacg ctgtagtgta tattcatttc caataaaatg aacgatgtcc cctgccgtaa
     4081 atttagcata ggggccaggt gaaacaagca gttcttcatt atgagaaatt gcgataactg
     4141 tggcgccagt cgcctgccag atattcaact cgctaattga acggtccaga tgcattgctt
     4201 tttctgataa agataattca aacggaagta aaggattcat cttatgtatt cgcttcgttt
     4261 gattgaccag ctgatccaat aattgagaaa aatgattcag ctcatcttgt tgcttctcca
     4321 cacttgccat gatttctgct tttgtatctt ggatggattg tacatcttga tattgcaata
     4381 ggaaatcttt tgctttttcc ttcgaagcaa caaatgcccc acttccatgc cgtacttcca
     4441 tgattccaag atctaccagc acattgatag ccttgcgggt agtctcagga gaagcattgt
     4501 acgtactggc aagtgttgag cgggcatgga tcttctcacc cactttatat cggttttcaa
     4561 cgatacgttc ggctaaatca acagcaatct gctggtagcg cgggcggacg atttttttct
     4621 ttccagacat tttctttcct ctttcttttt ttactttccc tcatcttact atgctctagc
     4681 aaaataagca aaaaagggga gaatagcctc ttgcaacgac tgatcatcgc ttgatctgtt
     4741 ccttttttac tgggcaagct gtttgacagt catcacaatt cttaccggat tttaaacgag
     4801 tatagacaat tgcgcctgca cttccaaaga ttaacacact taataagaat gtcgccatcc
     4861 tattcctctc cttctaatga caatatagta taaaactggt tcttggattt tggctgacgg
     4921 aacaaaacat aaacgagtcc tatcatcagt aaaatagcaa gatacgtccc taacgctacc
     4981 tgtccaccct ccaataagtg tcccaactga taaatcacaa aactaactaa ataagctaaa
     5041 ccgcattgat agcctatggc gatccaagtc catttcttgg cattcatttc ccgatggata
     5101 gccccaattg ccgcaaaaca aggcgcacaa agtaaattga aggctaacaa tgaataagct
     5161 gctgcgggag tatatgtgtg ttgtagcact tgccagattt ctctgccatt ttcggatact
     5221 tccgcatgac caaacaaaat accaaatgtg ccaataatat tttctttagc gaccaatcct
     5281 gtaatcgctg cgactgttcc ttgccagttt ccccaaccaa gcggctgaaa aacaactgcc
     5341 aggattcgcc cgaaaaaggc taagatactt tggtcttcag aaacaggttg agcataaaaa
     5401 ttataagaag agctgaacca gatcacgata ctagaaacaa agataatcgt tcctgctttt
     5461 ttaacaaaag acttgctgcg gtcataggtt tgtcttagga ccgctgacca ttgaggaaaa
     5521 tgatatgcgg gcaattccat gataaaagga gctgtatctc cagcaaacaa acgtgttttt
     5581 ttcaagctga ttccagagaa aataattgca gtcataccaa gaaaataggc agaaggagct
     5641 acccaagatt gatgaggaaa aaaggcccct gctactaagg caataatcgg caactttgca
     5701 gaacaaggca tgaaggttgt gaccatgatc gtcaaacgac gatcttgctc gttttcaatc
     5761 gtgcgactgg ccatgacgcc tggcactcca catcctgtag caatcagcat aggaatgaag
     5821 gattttcctg aaagaccgaa tttcctgaaa agtcgatcca tgacaaaagc gatcctcgcc
     5881 atatatccgc agtcttccag aaagctcaag cataaaaaca atataatcaa ttgcggtaga
     5941 aatcctaaca ctgctcccac gcctgcgatg atgccgttca agatcagctc ctgcatccat
     6001 cccgctactt gccattcagc taacaatctg ccaacatgtt ctggtaacca tgtaccaaac
     6061 aatgtgtcat tgatccaatc tgtccccatt gttcctactg tttgaatagc cagataatag
     6121 atcagccaca tcacaaatgc gaagatcggc aaggctaacc aacgattcgt tgcaatctga
     6181 tcgattttgt cactcatcga taattggaaa gaatcatttg acacaacaca gagtgtacat
     6241 agttgggtaa taaattcata acgttcattg accagaatcg tctcactatc gtcgtccaat
     6301 agtttctctg ttaactggat cagttgctcg atctcttttt gctgagaaac tgctatctcg
     6361 taatcctctc ttaccttctg atctcgttca aaatatttca ttgcatacca gcgtttcttg
     6421 ccgctttgtt ctcctagtac atcttcaatt tcatgtaaag ctgcttctaa acgtggatca
     6481 tatgtacagg gaacaggaat agaaggccgc ttttttgcag actcctgaat cttttcgatt
     6541 agtttttcga ttccttgctt tttcaatacg ctgattggta aaactggtac accaagacca
     6601 taggaaagct tttctagatt gatttttttt ccttcttttt tgatgacatc catcatattc
     6661 agaccaatga tcataggaat gtctaaatcc aataactgcg tagttaaata aagatttcgt
     6721 tctaaattgg tagcatcaat aatgttgatg atcagttcag gctgttcctg aaccaaataa
     6781 tctcttgcaa tcacttcttc tggtgtataa ggagacaaag aatagatgcc gggtaggtcc
     6841 tgaatatgaa tgttcggatt ttttttcaac gatccttctt ttttttctac tgttacgccc
     6901 ggccaattcc ctacatattg attcgaacca gtcaagagat tgaacaagct agttttcccg
     6961 ctgtttggat ttccgactaa cgcgatattc attctttttc ctccctttgt atcaagatta
     7021 gttcggcctc tgactttcgt aacgtcagtt cataccctcg cactgtcagt tcgattggat
     7081 ctcccagagg ggctagtttt cggataacta tttttacatt tcgcgtcaat cccatatcca
     7141 ttaaccgtcg cttcactgcg ccttgtccgt ggattgctac gacgatccca gtctctcctg
     7201 cttttaattg atcaagagaa gtccagcttt cagaggaagc agtcttttta ttgacaaaga
     7261 tttgacctag tatttcccga cttactgcca ctcgatgact ttttaccagt aaaatggcat
     7321 tgttgcctga taaattgacg acgacgattt tacttccggc aacgatcccg atattttgca
     7381 aatggcgttt tgttttttct tctgtttcga ttcgttcgac ttgataaacg aaattctgct
     7441 ttgcctgcgc taaggtctgc atattgattc ctctcttgtg aaataaatca tgtattaaaa
     7501 tcattcatta tatgttctaa ttttcttacg tgttacgcaa ataaatgaga atcattctca
     7561 catagaatac tctcggtaat tgagaatgtc aattatactt gtgatattat gagcgaatct
     7621 ttagtattga tagataaaaa tgagaaaaaa atatttcttc atagttttcc acaatttttt
     7681 ctctattaaa tttgctattt tcccacacga tacttgtttt cagctctttt ttcaaaaaca
     7741 caaaacttag tattccacag tttccacaac acaatatgta gtgtttgttg tttgactttt
     7801 ttactaagag ttttatacta tctattatag ataataagaa tgtgttagag gaggcactca
     7861 ccatgaacgt aaaaatcttt tctaaaaata attgtatgca atgtaagatg gtcaaacggt
     7921 ttttaacaga aaatcaaatt gcttttgaag aagtcaatat cgatgaacag cctgaagcag
     7981 tagactggtt gaaagcacaa ggattccaaa gtgttcctgt cattacttct gatgcagaaa
     8041 cagttatcgg attccgtcct gatcagttgc gtaaacttgc cagctaacat gactaaaaga
     8101 atcaaaacgt gatacatgga tttgaagggc ttgagacagc accagttttt gtcgcaaact
     8161 tccaaatcct tttttaaagc ttaaaaacaa tgcaatgatt tgcaatcaag catcatcaaa
     8221 tacgaaatga aggttgggcg tacgtatgag tcttaaaaat ttacaagatg tcagttattt
     8281 caagctgaat aatgaaatca atcggccagt caatggtcaa atccctttga ataaggataa
     8341 agaagcttta gttgcctttt ttgaggaaaa tgtcaaacca aatacgatga cttttccatc
     8401 tattatggaa aaaatcaatt acttgatcga tcaagaatat atagaagcag aattcattca
     8461 attgtattca ccatcattta ttgaaaagct atatcaattt ttggacgagc aacatttttc
     8521 tttcaaatct tttatggctg cttacaaatt ttattctcaa tatgcgttga aaaccaacga
     8581 cggttccgct tatctggaaa cttatgaaga tcgagtagcc ttcaatgcgc tttactttgc
     8641 gaacggggat gaagaattag cacttgctct tgcagacgaa atgatccatc aacgatatca
     8701 gccagctaca ccttctttct tgaatgctgg acgtaaacgt cggggcgaat tggtgtcttg
     8761 tttcttgact caagtcactg acgatatgaa ctctatcggg cgctcaatca actctgcttt
     8821 gcaattgtcc cgtattggtg gcggcgtagg gattacttta tcaaatcttc gtgaagccgg
     8881 cgctcctatc aaaggatatg agggtgctgc aagtggtgtc gttccagtca tgaaattatt
     8941 cgaagatagt ttcagctatt ctaatcaatt aggtcagcgt caaggagctg gcgtggttta
     9001 tttgaacgtt ttccatccag acattattgc tttcctttca gcaaaaaaag aaaatgcaga
     9061 tgaaaagatt cgcgtaaaaa ctctttctct tggcgtgatc gtacctgata aattttatga
     9121 attgacacgt aaaaacgaag atatgtattt atttagtcca tatagtgtcg aaaaagaata
     9181 tggtgtccca ttctcttatg taaatattac agctgagtat gatcatctag ttgcaaatcc
     9241 aaacattcgc aaacaaaaaa tcaaagcgcg tgatcttgaa aatgaaatct caaaattaca
     9301 acaagaatct ggctatcctt acgtaatcaa tattgataca gcgaaccgcg agaatccaat
     9361 cgatggcaag atcatcatga gtaatttatg ttcagagatt ttacaagttc aaacgccttc
     9421 tgtgattaac ggcaaacagg aatatgaagt attaggaaca gatatctcat gtaacttagg
     9481 ctcgacaaat atcgtgaatt tgatgcaaag ccctaacttt ggtcaatctg tgcgtacgat
     9541 gactcgtgca ttgacattta ttacagatgc atctgatatc gatgtcgtac caccgatcca
     9601 aaacggaaat aagctgaatc atacgattgg attaggtgcc atgggcttac acacttatct
     9661 tgcaaaagaa caaatggaat atggctcaga ggattcatta gactttacaa atatttactt
     9721 catgctgttg aattattgga cacttgttga aagcaacaat atcgctcgtg aaagaaaaca
     9781 agttttccat aattttgaaa aatcagctta tgctgacggc acttatttcg aaaaatacgt
     9841 aactggagaa ttccagccaa aatcagaaaa agtcaaagca ttgtttgaag gcatttttat
     9901 cccaactgct gaagattgga atgctttgaa acaagcagta atgaaagatg gactgtatca
     9961 tcaaaatcgc ttggcagtag caccaaatgg ctcaatttca tatatcaacg atacgagtgc
    10021 aagtattcat cctatcactc gcatgatcga agaacgccaa gagaagaaaa tcggtaaaat
    10081 ttattaccca gcgccttatc tatccaatga gacgattccg tattacactt ctgcttatga
    10141 tatggacatg cgtaaagtaa tcgatgttta tgcaacggca caacaacacg tcgatcaagg
    10201 catgagtttg actttattca tgcgttctga aatcccagaa ggcatttatg aatggaagac
    10261 aacaacgaag caaacgacac gtgatttgaa tattttaaga cattacgctt tccacaaagg
    10321 aatcaaatcc atttattacg tacgtacctt tactgatgat gcagaagaaa tcggaagcaa
    10381 tcaatgtgaa agctgcgtga tctaatatca tagatagatt cgcttattca tattttaatt
    10441 aaaggagcat tctcatgtca gaaacatatt atgaagcaat aaactggaat gaaattgaag
    10501 atatcattga taaatccact tgggagaagc tgacagagca attttggcta gatacacgta
    10561 tcccactttc aaatgaccta gatgattggc gtacattgtc tcaattggaa aaagatacag
    10621 taggacatgt atttggcggt ttgacactgc tagatactgt ccaatctgaa agtggcatgg
    10681 atcagttgcg taaagatgtc cgcactcccc atgaagaagc cgttttgaac aatattcaat
    10741 tcatggaatc cgttcatgcc aaaagttatt cttctatttt tagtacactg aatacgaaaa
    10801 aagaaatcga agaaattttt gactggacaa atacgaatcc ttatctgcaa aaaaaagctg
    10861 agcgtatcaa tgaaatctac aaacacggta caccgctaga aaagaaaatt gccagcgtct
    10921 ttttagaaac attcttgttc tattcaggtt tttatactcc tctttattat ttaggaaaca
    10981 acaaattggc taacgtagca gagatcatca aactaatcat ccgagatgaa tctgtacatg
    11041 gaacctacat cggttacaaa ttccaattag gattcaacga gttacctgaa gcagaacaag
    11101 aaacgttaaa agactggatg tacaatcttc tttatgagct ttacgaaaac gaagaacgtt
    11161 atacagaaga attatacgat ccaatcggct ggacagaaga agtcaagaca ttcctacgct
    11221 ataatgccaa caaagcattg atgaacctag ggatggatcc actctttcca gatactgcca
    11281 atgatgtcaa cccaatcgta atgaacggga tttcaacagg cacaagcaac catgacttct
    11341 tctcacaagt cggaaacggc tatttgttag gtcatgtaga agcgatgaaa gacgacgatt
    11401 atttgattgg attggaataa ggatttattt taattcaaaa atttttcgaa aaataaaaag
    11461 agtttagcgt acagatattt taaatgtaca ctgaactctt ttttatgctt attcacttgt
    11521 ttcttccagc acttttggac tgtctaactt ttccgaaaca tgttccattc ttctcaataa
    11581 atagattttc cggatattta atattatcgt cttaatcact gcatagacag gtatgcaaat
    11641 cagcatgcct aatattcctg cgacacttcc tgctccaatc aataccaaga tgatcgtcaa
    11701 aggatggata ttcattgatt tcccaaataa caacggcttg atcaaatttc catctaattg
    11761 ttggatcact aaaatcgaca acgccatata caaagcttga aatggagaga taaacaaacc
    11821 gacaatgatt gctggtaccg ctccaataaa agggccgaca tacgggataa tattcgtgat
    11881 tccgcaaatg accgcaagca acaagctata aggctgatcg aatattctca ttgccaaaaa
    11941 gctcattaca ccaataatca aagcatctaa gatcgtactg ctgatatagg cagataatgt
    12001 ttcgtttaat tcttttaccg tttggcgtaa ctctttacga atcgctttcg gaaagaagtg
    12061 agacaacgca tcaataaatt tgtgcccatc cttgaacata aatagcaaaa taaatggaac
    12121 ggtaaataac aaaataaaaa attgcatcat aaatgaaatg atttttgaga cacttgcaga
    12181 taaactgact aatatgatat tgaacaaatt actaagagat aaatttgctg atgctaattg
    12241 ttcttccaaa ttgaaatttt taaactcttc ccgctgcgcc aattcattta accatttgct
    12301 tgtttcatct gcatacattg gcaagctctg ggtcaattga attgtctgtt ccattaactg
    12361 aggcactaca ttcatgacag ttaaaacaac taaaatgatt acgaccaaaa acgtcaataa
    12421 aaaaccgaac actctcggga tccgatgctt ttcgagaaaa acaacgattg gatcgaacat
    12481 ataatagata aaaccagcaa caagcaatgg taaaaaaatt gccgaaagca ttttcaatat
    12541 tggactaaaa atattcggca tttgtaaaat aaagtacacg ccgatgatcg tgagtactag
    12601 ctccactgtc cagaaaaata attttgattg tttaaaacgt gattgcatac atctcctcca
    12661 aggtcctttg atacttctat tctatcattt aacaaagagt tacgcgctga gattttcatt
    12721 tgcttaaaaa aaacaaaaaa aataaacata ttttaatgag tagacgagta cttaaacaaa
    12781 aacacggttt ggagtagtga caaaaattat actgcagtcc ctttttccaa ataaacggtg
    12841 ttcaaaagct tgcatcttcg taataagccc agccaaacga aaaatataag gaactatttt
    12901 tatttggctg ggcttattac ttgatgtagg acatctattt tacaacctca gatcatttat
    12961 tgcgcaatac tgactaaatg caactttgtg atatttttgt gtacggacaa ctcctcgatc
    13021 acatcggcat aagtgagccc ttttggaaga tcaatcgtat agatattttt gtacactcgg
    13081 tagtcatcaa cgaattttac gtcaaaattc acatcttcga ttacgatatt tttctcttca
    13141 aaataatcgg taagaaaatc tttggtttct tttctatgga taaattggat ttctaacttt
    13201 ttagtcgtag gtacatggat cacgcgtttc actactgtga gtgcaatagc aatcccgata
    13261 aaactgatga tcgcaatgga atagtaaccc atgccgatgg cgatccctaa tccagcaatt
    13321 gcccataaag aagcggcagt cgtaagtccc gtcaccgatt gtttcgtgac gatgatcgtt
    13381 cccgcgccta agaaaccgac accactcacc acttgagcaa tcagccttgc ttcatctgaa
    13441 cgaatcgttc cagcaagctg tggatactct aaagcatctc ttaatgcacc actagcgatc
    13501 tcaacttgga tcaatgcaat gatacatgct cccatacaaa ctaaaatatg cgtacgcata
    13561 cctgctggac gatttttata ttgtcgttca aaaccgattg ctccgccaaa tagcatagcg
    13621 atccctaaac gtaaaataat ctctggaatt gtcaatacca tatcattcat ctaaacaccc
    13681 tccagtccaa tatatcatgt ctgaaaggag cagacaaaaa aagtgcaatt tttacctctt
    13741 acgaaaaaag gactgtgaca aaatttttgt catagtcctt tttatgaata aacggtgttc
    13801 aatcagctga ccctcggtat tactcaacaa acggcaaaat atggggagct atttttcctt
    13861 tgtcttctct aatactcagg tcaaaacgct gatttcacaa cctctttagc ctactattga
    13921 aaattacaac ttacttgctg cttcttcatc gatgatcaca acaacattgt cgtgattttg
    13981 caatacactt gctggcaaat gattcgttac aggtccttca atcatccctt tgattgcatc
    14041 agctttgctt tcgccaaatg ccatcaagac gatttcttta cttttcataa ttgaaccgat
    14101 tcccattgaa acagcttttg ttggtacatc ttctgcttta tcgaagaatc gtttgtttgc
    14161 attgattgtt gattctgtta agtcaactac tgaagtcgtt ccgtcaaatg aagcaccagg
    14221 ttcattgaaa ccgatatgtc cattttgccc gattcctaag atttggatat cgattggatg
    14281 tgcatcgatg atttgatcat actttttgca tgctgcttcc agatcttctg cttttccatc
    14341 tggtacaaac gtttctttga atggtttttt atcaaataat tggacatcca tgaaatgacg
    14401 gtagctttgt tcatctgaac cgcctagtcc aacgtactca tctaagttta cagaagtcat
    14461 ttcactgaaa tccaaatcgc ttgaagtcat ttctttatat aaagtaactg gtgtgcttcc
    14521 tgttgccaat cctaatactt ttgcaccttt gttcatacct tctttgatga tttcaaatgc
    14581 tttttttccg ccttcttcag cgttttttac acgaatgatt tccatagtga ttgctccttt
    14641 atattttggt atagcccaat tatagcattc tttttttctt tttacaataa ttagtataga
    14701 ccaaattgca tttttttcat tttcttgaca ataaatagga atccgtgtta ggtttcataa
    14761 gatggttcaa tcaaacaatc catgttgcat gaaaaccaag caagtgattc actgtggacg
    14821 ctttatgtct gcagtttttt tatgaaaaag gagttattat gagtatactg acattaaaca
    14881 acattaccca gcaatttggc gacaaaatcc tctatgaagg tgtcgatctg caattaaatg
    14941 ctggcgaaca tcttgggctg attggacaaa acggagccgg taaaagtaca ttgattaaaa
    15001 tcatcactgg tgagatctta ccagatgacg gacagatcca ttggcaaaaa aatattcaca
    15061 aaggttacct cgatcaatat gtggaagtcg atgaaacatt aacaatcgaa gaatttctaa
    15121 aaactgcgta cgcaaaagaa tacgaaaagg aagaaaaaat tgcttttctt taccaaacat
    15181 atagcgagac cttcgatgat tccttgctgg aaaaagctgg gaaactccaa acggaattag
    15241 accaaggagt gttttatcaa atcgacacct tagttgctga gatgagcagc gggttgggga
    15301 tcgatgtctt aggtttagat tcactgttga aaaatctaag tggtggacaa cgttcaaagg
    15361 tgattttagc aaaactactt ttagaaaacc cagatgttct gatcttagat gaaccaacca
    15421 atcacttaga tgatcagcat atccaatggc tgacgcagtt tcttcaggat tttgacggtg
    15481 cgtatatcgt gatttcccat gaccaagaat ttcttgatca gatcaccacc catatcgcag
    15541 acatcgagtt tggaaagatt actaagtata ctggccatct aaaacaagca ttgaaacaaa
    15601 aggaacaaaa tcgcgaaagc tatttgcgcc aatatcatgc tcaacaaaaa caaatcgaaa
    15661 agaccgaagc ttatattcga aaatacaaag ccggttcccg atcaacgatg gcaaaaagcc
    15721 gacaaaaaca attagataaa atcgaacggc tgacgcctcc agcttcagca agtaaagctg
    15781 tctttgattt tccattctcg cctattgtca cgacattagc agtggaaaca actgatttag
    15841 tcatcggata tgatcgaccg ctacttgcac ccatcaattt gaccatccgc tttggtgaaa
    15901 atattgccat tcgaggcttc aacgggattg gaaaatctac tttattaaaa acactgatcg
    15961 gtgagattga aaaaataagt ggcgatttcc acttcccaga taacacaaaa atcaattact
    16021 tttcacaaga tctgtactgg gaaaatccat tagaaacacc actacaatat ttaagcagtc
    16081 aatttccaaa agcgactatc aaagaattac gtcgccaact agctaaagca gggttagtta
    16141 atcaattagc aagtgaacct ctcactacat taagtggtgg ggaacaaact aaggtgaaac
    16201 ttgcacagtt gacaatgaac caaggtaatt tactgattct cgatgaaccg acgaaccata
    16261 tcgatcaaga aacgaaagaa agcttacaag acgggataca gaaatttcca ggaacagtaa
    16321 tcatcgtctc tcatgaacaa gaattttatc aagatcttgt gcaacgagtc attgagattg
    16381 aaccaaaata aacgtttgat tgaatagttg aaaaaaagga gctgtgacaa aagttttgtc
    16441 acaactcctt cttctgaata aatggtggcc aattagctga ccttcggtat tagctcaaaa
    16501 aacggcaaaa tatgaggagc tatttttatt tggctattct tattacttga tgcagaacag
    16561 cttttcacaa cctcttttcg tctatttttt attttcgaga tttctctttc ctttaaaaac
    16621 aactgtttga aagaagaacg cacatccttg aaaaacaagc ccgctaaccg ttacacctaa
    16681 ccagccatac tgctgccaca ttgtcgtccc tagcaatgaa ccagccgcac ctccaataaa
    16741 gtacataaac ataaatacag acccgtggtg ctagttaatt ttaatatcct aaagaatatc
    16801 tcaaagttcc ttcaaaacgt cgttgtgctc gacttaatag aacactgtaa gccagtaata
    16861 tgctttcaaa tctactctct agccctttaa cagaacgtga gttgaaattt tgacaaccta
    16921 atttttctaa ggaagaa
//
LOCUS       DACCYH010000061        16795 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.062, whole genome shotgun sequence.
ACCESSION   DACCYH010000061 DACCYH010000000
VERSION     DACCYH010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.062"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(28..741)
                     /locus_tag="IVY85_001936"
     CDS             complement(28..741)
                     /locus_tag="IVY85_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8044949.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            916..2340
                     /locus_tag="IVY85_001937"
     CDS             916..2340
                     /locus_tag="IVY85_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8044950.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2639..3346
                     /locus_tag="IVY85_001938"
     CDS             2639..3346
                     /locus_tag="IVY85_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP8044951.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3333..3656
                     /locus_tag="IVY85_001939"
     CDS             3333..3656
                     /locus_tag="IVY85_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8044952.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3745..4782)
                     /locus_tag="IVY85_001940"
     CDS             complement(3745..4782)
                     /locus_tag="IVY85_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8044953.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5103..6086)
                     /locus_tag="IVY85_001941"
     CDS             complement(5103..6086)
                     /locus_tag="IVY85_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP8044954.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6501..7271)
                     /locus_tag="IVY85_001942"
     CDS             complement(6501..7271)
                     /locus_tag="IVY85_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8044955.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7264..8199)
                     /locus_tag="IVY85_001943"
     CDS             complement(7264..8199)
                     /locus_tag="IVY85_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8044956.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8515..10164)
                     /locus_tag="IVY85_001944"
     CDS             complement(8515..10164)
                     /locus_tag="IVY85_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8044957.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10281..12884)
                     /locus_tag="IVY85_001945"
     CDS             complement(10281..12884)
                     /locus_tag="IVY85_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8044958.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12896..13945)
                     /locus_tag="IVY85_001946"
     CDS             complement(12896..13945)
                     /locus_tag="IVY85_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP8044959.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13971..14285)
                     /locus_tag="IVY85_001947"
     CDS             complement(13971..14285)
                     /locus_tag="IVY85_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP8044960.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14301..14747)
                     /locus_tag="IVY85_001948"
     CDS             complement(14301..14747)
                     /locus_tag="IVY85_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8044961.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(14748..16724)
                     /locus_tag="IVY85_001949"
     CDS             complement(14748..16724)
                     /locus_tag="IVY85_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP8044962.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
BASE COUNT         4984 a         3414 c         2763 g         5634 t
ORIGIN      
        1 agctctgaaa aaataatttt cccgagctta tttttcaaca aaaacagctt caaaaacaaa
       61 gtcttgatat aaatgtctcg ataccgaata ctcgaaaggt ttaccagacg atagagcaac
      121 aactttgtct atctcaatca taaattcacc tggatctatt tttaatattt ttgcgtcaaa
      181 ttcattcgca gtatctcctt tgatacgtat gtgcgaagtc tttatttcca gacccagttc
      241 atttgtgata taagcatata cagatttctt taaatgtttt ggttccaagc ctggtatgac
      301 tgctaatggc atgaatgttt gctcaatcga gtatggttga caatcaataa aacggcttcg
      361 tacaatatta tatacatagt cttttacact gatacttaat ttatcagcta cttttttcga
      421 tggaagttca attgagaaag tatggatttc tgaatctacg tgttcttcac caaccacgct
      481 aaacaaacct tcgtttggac caagaactcg tgagttatca attggtgttc tgacgacttc
      541 agtcccatac ccacttctac ttcgcaaaac tccttcggaa acaagtaagt ctacgccttt
      601 ctttactgtt aacttgcttg tatgataaat aactgccaat tcatctcctg taggaagttt
      661 ctcaccaatt ttatactctc catttacaat ttttttcttt atatctaagt aaatttccat
      721 atacttcgca atttttttca atggtgaccc tccaatcaaa aacatatctt catctatact
      781 ttaacataaa taaaccgata aaatccacat caaaattata cacacaataa aaaagatata
      841 tatatttaaa caaaactatt tactttaagt tgtaagcggt gttatcatta agtggaaaca
      901 ggaggtaaaa aaattatgac aactatgaaa aagctaactt ttccagatga tttttggtgg
      961 ggttccgctt ggtcagctga gcaagcagaa ggaaaaggag aaacaggaaa agcagagact
     1021 atctgggatc gctggtttaa agaacaacct taccggtttt atcaacgaat cgattcctct
     1081 attacaaccg atcacatcca tcaatataaa gaagatatag aactaatgaa aaaaactggg
     1141 cataactcct ttcgcgtttc tatttcttgg gcacgaatgt ttccaaatgg cggtaccgga
     1201 aaagtaaatc ccaaagccat ttctttctat cgaaaattat tttccgaaat gaataataac
     1261 ggaatcaaag tatttgccaa cttatatcat tttgacatgc ctgctactct tcaagataaa
     1321 ggaggatggg aatcacgtga agtagttgat gcttatgttc attacgctga aacttgtttt
     1381 aaagaatttg gtgacttagt ttgtcattgg tttaccttca atgaaccact tgggcctatt
     1441 ttaggtactt atcttgaaga ttttcattat ccaaacctca ttgactttaa acgcggagcg
     1501 caagcagcat tcttcactat acttgcacac gcaaaagcaa tacaagtatt taaagaacta
     1561 gctcttccta gtaaaatagg cgttattcta aatttgagtc ctacttttcc tcgtagtcaa
     1621 aatcctgctg atgtgaaagc agcagaaatc gctgatttat tttatacgcg gagcttttta
     1681 gatcctatgg ttaaagggaa tttttctaaa aagcttatcc tgttattaca agaatataat
     1741 cagcttcctg aagattatac gaaagaagat cttgtatgta tctacgaaaa taccgctcaa
     1801 attcttggac taaactatta cgagccacgt cgcgtcaaat ctcgttcttc tatggtggat
     1861 acatctagtc catttttacc agagtggttt ttcgaaccac atatcatgcc tggacgacga
     1921 atgaacgaat accggggatg ggaaatttac gaaaaaggaa tttatgattt gtgcatggat
     1981 atcaagaacc attatggaaa catcgaagca tttatctcgg agaacggaat gggcgttgcg
     2041 gatgaagaac gtttcatgga tgatacaggg caaataattg atgattatcg gattgagtat
     2101 atcaaagatc atcttgctta cttatggaag gcaataaatg aaggatgtaa cattaagggg
     2161 tatcatcttt ggacatttat tgattgctgg tcatggatca acgcctataa aaaccgatat
     2221 gggctggttt cactcgacct tactactcaa aaaagaacaa ttaaaaaaag tggcgaattt
     2281 tacaagcaat taagtgatac aaatggattt agatacgatg tagacttgtt agttgaataa
     2341 tattctaaaa ttccacttga agtgaacaca ttagaaaaca agaataatga gaaataccaa
     2401 taacaaatac tttttcttca tacttcattt atacttgtca gtataagaga ctgtgacaaa
     2461 tgtagtgatc ctcagaagtt aggtctggaa atctaacttg agaagatttt tttaaactac
     2521 gtccagtctt ttttatttgt ttaaaattga agttgacaag tcttcattaa aaatttagaa
     2581 tattcacaag tttgacgaca attatgaaaa tttatgtcgt gctaaaggag ttttgttatt
     2641 ggatgaaaag ttagatataa aaactgcgat caaagacacg ttacctaccg tttttggtta
     2701 tatcggtatt ggacttgcat ttggtatcgt tgggaaagct gccgggtttc atccgctggt
     2761 cgtcacgttg atgtccctgc tgatctatgc tggttctgcc caatttatca cggtcagcat
     2821 gcttgctagc catagtccac tgctctccat cgttctctcg acctttctag tcaattcccg
     2881 aatgattctg atgagtatga cgattgctcc ttatttcaag aaaaatcggc tgcttcaaaa
     2941 tctgctgatt ggcacattgc tgacagatga aagtttcgca ctgggaatga ataaattgaa
     3001 ctatactgga caaaagttaa actttcgttg gatgaataca gctaattgga tttcttattt
     3061 gacgtgggtg ggttcttctc ttgttggcgc acttttaggg aattttatta ctgatccgaa
     3121 aaagtttggt ttggatttcg ccatcgtcgc tatgtttatc gggttacttt atcttcaagt
     3181 catttctgat agaaacacct ctaaacgatt acagttgatc ctgattggtc tcactttgat
     3241 tctggtatat gtcggactga tttttatccc aagtaactta gtgattttag ttgtaacact
     3301 gattgggtgc ggattggggg tgtggatcaa acatgccttc ttttgagttt acattgctga
     3361 ctattttagg ttgtacttta gcaacttggc tttctaggat cctaccattt gtcttactga
     3421 aaaaattcga tttgccgcaa ccgttgcttg agtatttaag ttttgtcccg atcgtcatta
     3481 tgtctgcttt atggttcagc agtctattta cccaaaatat cggacatctg ccgcagatca
     3541 atctcgaaaa tgctttggct tcagttccta cacttttagc tgctattctt tcaaaaagct
     3601 tattggtgat cgtcttggta ggaattcttt ccctttcgtt gattcgctta ttataaataa
     3661 aaaaacaaga gcttatggca aattactgtc atgcgctctt gtttttatca aagtcaaata
     3721 agaaaaaaat cggcgtattc ttgcttatcc gttgaacgga ccactaacaa cgacttttcc
     3781 taatatatgt cctgtttcta cgtctttggt tgcttgtttc aagttcttcg cattcatccc
     3841 atctgaatag accttgctca atgtactgcg gataatccct tcatttgcta aagcagcaat
     3901 gtgcgctaaa gcttctcctt gcgtttcgat ttcatagtta tagtctgttt tagcgaacat
     3961 atactcccag tcgaagctga tagattggtt tttccaaaca cctatatcta acggctcttt
     4021 gatccctacg atcgttccca catgtccaaa tggtcggaca aggtgtttga tttgatcaaa
     4081 gtagcgggta atatcgaata atacagcgac atactctact tgatcgattc ctaattcacg
     4141 aaaactttta cttaagtcgt gatgataatc gattggatag tctacacctg tttttttcag
     4201 ccattcaaaa ttcttcggac ttgcagtcgc gtagacttct aggccagccc aatgcgctag
     4261 ttgattcaaa atcgaaccga cgcccccagc tccgttgatc actaagattt ttttcccgcg
     4321 gttggcattt tcttctggag tcagaccaaa tttttcaaat aacaattcat aagcggtcaa
     4381 tgaggtcaac ggcaaggctg cggcttcttc atctgaaaga ttttttggtg caagagcgac
     4441 aatacgttcg tcaaccagtt ggtattcttg attgcttcct gctcttgtcg ttgtacctgc
     4501 gtaaaaaacg cgatcgccga cagaaaattt ttgcacttca tcacctacag caacaacttt
     4561 cccgactcca tcaaacccta agatacgtaa agcatttctt atcccgttat cttggcgaag
     4621 tttcgtatcg acaggattaa cagatactgc gttgactttt accagaagat cccgtgaact
     4681 gggtacaggt atctgttctt tttcatcaat aaagcttttt ggatcatcga tcgacaaccc
     4741 ttcataaaaa ccgactacct tcattaattc cttttctgac atacgaattc ctccttagct
     4801 tcatcaagtt ggagcatcca tacagttttc atttcaatat atatagtatg ctatttgttc
     4861 cacttttatt catcataatt attctcttac ttaaagtaaa ctatttgcac ttttttggtt
     4921 aaaaaagagg ttgcgacaag acttttgtca caacctcttt taaacggtgt tcaatcaact
     4981 gaccctcggt attcgctcaa aaaacgacaa aatatgagga gctatttttg tttggctgtt
     5041 cttattactt gatgcaggac agctttttca cgacctctaa ttcacggtgt tcaagcaaga
     5101 aatcattgtt cactgaaaag cgttggatgt tctttttcaa aggtgtctaa gatctctcgt
     5161 acttcatcag tagatttcgt attcatcaac tgaactcgca agtcatttgc tcctggaaat
     5221 cctttgatat agattttaaa gaaacgatgt agtcctgtga tggaacgcgg gatgatttct
     5281 gaataatgat cttgcagatc tagttgcaga cgaagtagtc cgatcagttc tttttctgta
     5341 tgttccctcg gttctttttc aaatgcataa ggattcttga aaatcccccg gccaatcatg
     5401 atgccatcta cgccatattt ttcggccaat tcttctcctg tctggcgatc tgggatatct
     5461 ccgttgatcg tgatcagtgt ttgcggagcg atttggtcac gtaaagcaat gattttaggg
     5521 atgacttccc aatgtgcatc tactttactc atctctttac gcgtacgcaa atggacagaa
     5581 agattggcta tatcttgatg aagtaggtga ctgatccatt cttccatctc agacatttcc
     5641 gtatagccga tacgcgtctt gacgctgaca ggcaaaccgc cagctttagc cgcttcaatc
     5701 aattgtgccg ctacttctgg gcgaaggatc aagccactgc cttttccacg ttctgctaca
     5761 ttagggactg ggcagcccat gttgatatcg attccttgga agcccatttc tgccatagca
     5821 atgctcattt ctctaaaaaa ttctggcttg tctccccaaa tatgggcaac catcggctgt
     5881 tcatcttctg taaaggtcaa tcttcctcgt acactgtctt ttccatctgg atgacagaaa
     5941 ctgtctgagt ttgtaaactc agtaaagaag acatccggtg ctgctgcatg ctttacgaca
     6001 tggcgaaata caacatctgt tacatcttcc attggtgcta aaacaaaaaa aggctttggc
     6061 agctctttcc aaaaattagt agtcatttat ttgttactct ccattctatc aagcagtgaa
     6121 gtcgcaatct ttctcaaaaa cattgtcatt atactaggaa acaaaaaaag aagcaaagtg
     6181 aacagcttaa aagatgtttc tgattgagca aaaaaagact gtgacaaaaa tcttatcaca
     6241 attccttttc cggaataaat ggtgttcaaa agcttgcatc tgcggtaata agtccaacca
     6301 aacgaaaaat aggaggagct atttttgttt ggctgttctt attacttgat gcagaacagc
     6361 tttttcacaa cctctccact aaacggtgtt caatcagctg accctcggta ttagctcaac
     6421 aaacgacaaa atatgaggaa ctattttccc gtttgtttct ctaatactca ggtcaaaacg
     6481 ctgatttcac aacctctttt ctaacttctc aatcctcttc ctttattgat caaataccaa
     6541 gtaacggcgt acaaaacaac gatgaatagc acaagtacga tcatcgatgt tgctactggc
     6601 acatcgattg ttccaaggaa tccgtaacgg aaaccggaga tcatataaac gatcggattg
     6661 actttcgaaa cagcttgcca aaatggcggc aacatagaga tggcatagaa aacgccacct
     6721 agataagtta acggctgcaa gacaaatgta ggcacgatcg atacatcgtc ataagactga
     6781 gcaaaaatcc cgttgagcag tcctgctaac gaaaacaaga ttgccgtcat caataatgta
     6841 atgatgacca tgaaccatga atacacatga agtggtacaa agaacaagga aatgatcgtt
     6901 acgagagagc cgacgagaat actgcgaccc actccgccaa tcacaaagcc ccaaatgatc
     6961 acatgcgtag gaacaggcgc taccaagatt tcttcgatgt ttttctggaa tttttgagag
     7021 aagaaggacg aagaaacgtt ggcatacgaa ctagtgatag cagacatcat aatcaaacca
     7081 ggcacgatga attccatata tgaaaaacca cccatgtcac cgatccgacc accgatcatt
     7141 tttccgaaaa taacaaagta caaggatgtc gtgatcacag gcggaaccag tgtttggacc
     7201 cagatacgca aatagcggtt cgtttccttg atagctaaac tttttaatgc agtaaaataa
     7261 agattaaaca tttttctcct ccgtatgatt attttcttct gtgattttca aaaacagttc
     7321 ttctaaacga ttcgacttat tcctcataga aagaactttg attccttgtc ttgtcagctg
     7381 atcgaagatt ccgttcaccc cttgatttcg ttcaacttcg acagcgattg tctgttcatc
     7441 ttcaaatgta caagcgtatc cttcaatcac aggtttggtc tcatatggcg ctaaatcaaa
     7501 gataaatgtt tcaaattgca gtttggatag aagctctttc atactagtat tctcgatcag
     7561 ttcacctgat tggatgatcc cgatgttgcg acaaagcatt tcagcttctt ctaaataatg
     7621 cgtcgtcaaa atgatcgttg taccattttc gttcaattct cggagaaact cccacatctc
     7681 gcgtctcaat tcaatatcga ctcctgctgt tggttcatcc aagatcagca aacgtggttc
     7741 atgcatcaat gccctagcaa tcatcagtct tcgcttcatt cccccggaaa gcatacgtgc
     7801 acggacattt ctcttttccc aaagattgga ctgcttcaaa tatttttcgc tgcgtttcat
     7861 cgcttctttg cgagagacac cgtagtagcc tgcttgattc acgacgattt gctggaccgt
     7921 ttcaaaggga ttgaaattga actcttgcgg gactaatcca atctgttgct ttgctcggac
     7981 tagatccgta tccagatcat aaccgaagac attcactttt ccagatgttt tattgactaa
     8041 agaagtaatg attccgatcg ttgtagactt cccagctcca tttggtccta atagcgcata
     8101 gaaatctcct tctttcacgg aaagatcgat tcctcgcaat gcttcgaccc cacttccata
     8161 aacctttttc aaattattta tttcaagcgc atttgccatt tatttcctat ctccttatag
     8221 acatgattct ttattgtatc atggaataca gcagatgaaa aaacaacttg gttccagcga
     8281 aaaatcaggt tgctgggctc aggaaaatct cctaatgaaa gacatcaatc ggacgaagtc
     8341 agacaacctt ttcagaaaaa acaaatcgaa gataaaggat tactattcat atgtgcaaca
     8401 aaaggagcgt gagacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac
     8461 ggcggaagca aaagccaaga tttttgatcc aataaatact ttcgatcact tcgcctatcc
     8521 caatacatgc cactgccgct tttcatcaaa taacgcggct tccgtttttg cggtttctac
     8581 taaagtctct gggtaatcca aaactttcag caaggcaatc gcatttcggg tccttgaagg
     8641 tccttctttt aatttgaaat caaaactgat tccttttcct tctgttactt gttcttcaaa
     8701 gtggacattc tcacaataat tctttaaaat ctctgtcaat tcgatatcat gtgtggcgac
     8761 aaatgctaaa gatggatact ccgcaagcca ccgaacgact gaagcggaag aagcaatccg
     8821 ttcgatcgta tttgtccctt ttaaaatctc atctacgaag cagtagcagc gttctttgga
     8881 agccacttgg tccaacaaac gtttgatcga tttgatttct gaaacaaaat aactgtcgcc
     8941 ttcaaataaa tcatcttcga ttgccatgga agtcaacaca tgtccaggtt gcagggtaaa
     9001 ctgttctgca atcgctgttt ggatcgtctg tgctagaata cagctgatgg ctatactttt
     9061 gacatacgta gattttcctg atgcattgga acccgtgact aatgtattct tctgccagtt
     9121 gaccggattc actaccgctt cttttaacaa tggatgatag atatctttcg ctgtcacacc
     9181 gccctcagta aactctggtc ggcaagtgat gggcatataa gtacggtaat tcaatattgc
     9241 agcggctact tctaattctc ccagcaattc ccacagcttg atggcttcag ttgaatgctt
     9301 ttctaattta gtgagaacga aatgatagga gataaatggc agcatgaaca tgatgttgac
     9361 atattcaaaa atcaactctg cttcactccc actcttcata cgaaatgaaa aacctaattt
     9421 agggatgtct cttaatggtt tagtttgctg tttcagctct tcttgaagtg gtgtcggcag
     9481 ttttgctagc tgattggcgg ctgaaatcgt tgaaaccaaa taactcatgc tattcagctc
     9541 tgtttctagt gtttgtttct tcactagata atagaccgta ttgaatacta acccaccgat
     9601 tgccaaatag attcctagta cttgtcctaa aagaagcaaa cccaaaccca ccagcggaag
     9661 caagccaaga aaaataaata aacctacgcg tccaatcttt ttactttttc catccgctag
     9721 atattgtttt gcaaaattat gatctttttt tcctaaacga gcgaattgat attggatttt
     9781 ttctctcagt tgaggatttt cctgataaaa agcaatcagt ttttctaact gctgatcttc
     9841 accaaaatca aagctgcgta aacgttgata caacgcttct gaacctacac tagaataggt
     9901 actgttgatt ccttcgaata cttcgaacat atccaggtca taccaagtaa gatcatcgat
     9961 ttcgctatcc catgaacgga acttcttctc cgtgagccat gcttccttca agctttcttc
    10021 tttatcaaat ctcggttgat aaggaatttt ttcccatttg ctacgaactg tttcctttaa
    10081 tttcaaacgt gaaaaaatgt ctaaagcaac taataaaata acagctgcaa taataattaa
    10141 aacgataaat tctctttgac ccatatccct ctccgttcct ttatttgtta gctttagtat
    10201 agcactgttg cgctatttga aaagagggta aaaaaacacc tttcaaaata aaaggtgttt
    10261 ttttcttgta tattacatat ttatctctca tttatcaaaa ttgacaaata atcataggga
    10321 ggaattgttg ttaattcttg tttattctcc agtgcattga caactgtccc aagggaaagg
    10381 atagcctcag gtactatttt ttcttgttct gttggattag ctaagcgaag aacaaatcct
    10441 tgttcatgaa aataagaagg ataaatcgat gatacaatta agtcttcagg taattcaatc
    10501 aatgaaaatt tacgaggaac atcaagttca ttttgtaatg gccaaatctt gttatctaac
    10561 ctgtgaataa atacattcag tttctgcttt tgataagaaa tagattgttc taaccgtttt
    10621 tctgcctgtt ttgccacttt gaactcatta aatcttcctt cttctacatt aaatccaaaa
    10681 gtgactttat actcacctat ctcttgagct agcggtgttt ccatcatgac atgcccttca
    10741 tttgtcgtat ctcctgaagc tcgcccaggt cgccaagcaa gattaggttt tccaagttcc
    10801 cctgttgttg aaaataaggt tatgattaat tgattttcta tacgttcata ctcttttaaa
    10861 ccatctgcat aaaacgtaaa ataatgatca ttttctgcca cagagacact cttatcgaat
    10921 atttctaaat taacaggttt ctctaccata gtttctgacc aattcggagg aatgacatca
    10981 ggggtatttt caataaatcc gttttgaatt tgtgcaattg atttttcaga tttatctaaa
    11041 acattaattg ctaagcgcat acgatgcgag taaacagtat tatgaatttt gagtgtgcct
    11101 tcaacgattt gactttctcc cagactgaac gatatttcat aagatatttc ttccatttcg
    11161 ggtttttcag ataaacgatc ttttaaatct ttaggaagtt gagcttttcc gtaaacaact
    11221 aatgtttctt gtaaagaatc tttatatact ttagctgttt ctaattttag agatagttct
    11281 gtatctcctt ctaatggtga ataatcatac gtatcgccat catttgctga atcgatcaaa
    11341 tgaacgaaat caagatattg tctatctcca actatcaaat tcaattgccc atctttaaaa
    11401 gaaagtttat atttattgtt acttatctca gttttgtcta tctcgattgc tgtttctaat
    11461 ttttcattat ttttctcaaa ttcaacaaca gtgtatccta gtgctggcac atttacattt
    11521 atttttatgt caagttcata ataagctggt tcatcaaaat attcgaaacc tgtagcaatc
    11581 atccgataag catttttccg ttctggaaaa taaacttctt tttcaatgat attataggga
    11641 ctacctttga agtgtatttt ctttgaatca gctactacat gcacgatttt cgatccacta
    11701 taattctcag tcgttgtgtt gaaaacaagg acttcattcg attttagctt taatgcatca
    11761 gccatccgtt ttacaataag gttttcgatt ccgtcagcta tctcattagc ttctttgatt
    11821 cgatggaaaa tatcttccgc aacattatct gaaacacagc caccaatact atcatgagcc
    11881 tggttttgca aaatcttttt ccataaccga cgaagtaggc ctttactgat atggatacca
    11941 tttctttctg caatcagcat gagcggctca actcgtttta gaattttttg ttccaattta
    12001 aaattttcta ttttcatttt tgttcttact gagccaatag aacgatgaac acgagagtaa
    12061 accggctgac gaagttcacc tttatattca ggtaattctt ttttgtttct gataacttct
    12121 acataagaag gataatctga aataatattg tggaatttac tgtatttatt caacttctct
    12181 acgacttgat caaaatttaa aatcattgac ttttgatcta cacccgaagg tactaaaata
    12241 tctttgtttt ttccttgcat atgaagcatt tcaactgctt catccaactt ttcttttgca
    12301 aattcaggaa tatctttaat tgcgtctaag gtcatcattc cagtcatata accaaatgga
    12361 aaattcatgg cataaacaaa tttatctccc aatcctttcc agataaaata aggagatgga
    12421 accatcgttt caaagtttaa tcctcgccaa cacatgaaat tatcgatttc tacttgatgc
    12481 aaaagtgtag gcaaattcgc attgaaccca aacgtgtcag gcaaataccc taccatcatt
    12541 ggatctccat atttaaggat cgtatcactg attccaatca ttaagtttct taaaattgat
    12601 tcagcatcca ctaataaggc atctgtttgt gcataccacg gtcccacgaa tagttgtcct
    12661 gttttcatca gtcgttggat aaccttaagc ttttgcggat tgatctccaa atactcatcg
    12721 acgattgaag tttgtccatc taaacaaaag tttactttag ggttcctttc caattcatct
    12781 agaacttcag taaagacctg atcagacaat acgatggcat cttcatttgt aaaataccat
    12841 tctctgtccc agtgcgtgtg ttgaattata tgtgcattta ccatatcaga ttctcctact
    12901 ttccttcttc tactgaattt acttgttgtt cttttttata cttgatggat tttagtgcat
    12961 tgaccattaa cgctgtaacc actcccccaa taacaattga tactaaatat aaaggtatat
    13021 tcgtaacaaa aggaattgcc ataaaggttg aaagcattgt ttcttgagta acaccaaaaa
    13081 atccatttag accacatgct actgcagtgc ccaccacgat gcttggtatt actcgtaaag
    13141 gatctttcac ggcaaacggg atcgtccctt cagtaatccc cacaaggccc ataatcatag
    13201 ctgtctttgc attatctcgt tccgattcat cataaagttt tttgtttaaa attgttgcta
    13261 aaaacattcc aaatggcgga atagcaattc ctaataacat ggctgtacta ggtccaaagc
    13321 ttccgcttga aaaagaagca accccaaaag catatgcgat tttattgata ggtcctccca
    13381 tatcaaacgc catcattcct cctaaaatag ccgacatcaa agcagcattt gctcctgaca
    13441 tgccttctag ccaattagtc agtcctgttg tcattgcttg aataggcgta gctagtacat
    13501 accataatat gattccaaca actaatgtag taacaaaagg aatcagcata attgatttta
    13561 gactaataac ttgcatcggt acttttactt ttttcaatgc attagctaaa tagccacaag
    13621 ctaatccagc aaccattcct ccaataaatc ccataccaag attttgacac atcatcccag
    13681 tgataaaagc tggtgctaga ccgggacggt cagcaattga atatgcgata taggctgaaa
    13741 tcatcggtac gataaactgt aatcccgtct gaccaatcat tcgcaggact ccccagaatg
    13801 tatccgtctg gtcccaaaca cctgtgccac caaaaatgac tgcaatagaa gttagtaacc
    13861 ctgctgctac gacaagcggg atcatatagg aaaccccact ttggaaatga tttagaattt
    13921 ctcttccttg tttcttgaat tccattagta actcccccac gatatatttt ttatttgtta
    13981 attaatgcaa ttgcttcatc aatcgtctct tcaccatctt ttactacctc tgaggtccct
    14041 actgttaaaa tcggtttacc gtcaaagcga cctcgttctg aaacatttgt atctactgca
    14101 aatatgacaa catctgcctc ggaaacatcc gaaattgaaa gtttgttttc tatccccata
    14161 gccccctgtg tttctacttt aatatctaac cctttcttct tcgcaaactt ttctaaatta
    14221 gcttttgcca tataagtatg ggcaatccct gcgatacatg ctgttactgc tactattttt
    14281 gtcatatgaa attcctcctt ttaaaatgat agagctttta ccaattcttt tttatttctc
    14341 gaatctttta ttcttttaac aaactcgtca tccatcaatc ttccagataa ttctgctaaa
    14401 atacgtaaat gattagtacc tttatcagtg tcttttattg ctaacaaaaa gacgagatca
    14461 actggttgat catctaatgc gccccattca acagaccgct ttaacttggc tacagccact
    14521 gttgattcaa ttactgctag gcttttgcca tgaggaatgg cgatattttt cccaattcct
    14581 gtggtcatat ggttttctct gtcaaatacg tctttttgaa attgtttgtg gtcagacaca
    14641 aacccattag tctctaacaa tttcgataat ttttcgacta cacttttttt atcagatgca
    14701 tttaaatcta tcactatatt atttatattg attttttcac tgatcattta taagccttct
    14761 ttcaatatat aaactaattt ttcaaacgac gaactcgttt ttaaatttgc taataactca
    14821 tgattcgaag cgatttcatt gaatgtttca taaattttat tcaattcagg ttttactttt
    14881 tcattaagtg caaaaaagaa aacagaatga acaagtgtcc catcccaatc aattccttct
    14941 ggattaacgt agacacagat tactggcttt tttataaaat ctacttcgat atgcggcata
    15001 gcaaaatcat tcatcgctgt tgaagatagt ttttctcttt tgattgctcc ctctagtatc
    15061 ccttctttcg cgtaattttc ttttaccaat tttttggtaa ttatatttaa aacgttcact
    15121 tgattatcct ttatttggtg ggtaaaaaag caaaatcgtt tatctaataa atttagaaaa
    15181 gcatcttctg tttgttcatt taactgtaga tactgtttta cttttctgac atctgcccct
    15241 aacatcaaag gactaacaac aataacagga aaatttgtat tttcaatcgg aatcgtgctt
    15301 ataacaagtt catcagtgac ttttctatct tgtagttcat tgatgcttag tacatcttct
    15361 atctcaacca tatctgcaaa accgttattt attctttgct ctaataattt tgatgttcca
    15421 tatccgctac tacaaactaa tatcactttc gttttgtcag gtttgtaacg atccaaaagt
    15481 gattgaatat ggagtgttac gtaagcgatt tcatcttcat taaaatgtat acagaatctc
    15541 tcttcgattt cctctaataa atctacactt atcataaatg accttttaaa agaatatttg
    15601 attttgtccg tataagggtt atagatattt actcccaatt tgagtctttt aactgctgca
    15661 ttgagatgta atactagatt attgatcaat tcttcatcag gataaaattc atttaattta
    15721 attcttacta actcttgtag ctcaacaccc atatctaaat aggaaagctt cgtttgatct
    15781 aagtcgtgat tcatagcatt tttttcaatc gaagtaatgt gaatgtcaac ataagcttgt
    15841 tcatctattg gcacaataat tgaaaatgct ttttcaatca ttttgattaa aataagtgta
    15901 ttctttgaga tttcttcttc agaattatta ctacgtgata agaaattgct tgtttcaatc
    15961 ctttctatcg caatagcaag gtggatagct aaagtttgaa attcataatc tgtaaatttc
    16021 aattgtgtct tattcatgta tcgattaagc aattcggcta cttttttcaa tatgtcctta
    16081 tttaagaaat tattcgcttc accaatgaaa ttgatattca agtctaagag atctgtttta
    16141 ctgttagatg ctgtaaagcc accccaataa tagttcacaa cttctgacat caaccttctt
    16201 ttactttttt catcagcttt gatgcgcacg ccatttcgat ctgaaactaa ggacatccca
    16261 tgcttgttta gtctttttct aacatctttc attgtgcttt ctaccgtcga cctgctcacg
    16321 tataattcgt cagcaagatt ttgaatcttt agagaatcat tagtattcaa taatttagca
    16381 aacacataag ttagacggtc ctccggagag tctgcgatat tgattttatg actggataat
    16441 aaatttcgaa gtgtttcttt ttttccaata agcgaaacgc cttttttagg ttgaaccaac
    16501 atttgtacat ctggcatttt ttggattatt tctttcaaaa ttaaaatatc tttgctaatt
    16561 gttttcgtag atacatataa ttgttctgat aattctttgt aagttgtgtg ctcattttca
    16621 atcaaaaagt atagtatttt catttgccgt tcgctaaatt cgatgcccat tatcttcacc
    16681 tcctgaatac agcttaagtg tacaattatt atctctattt tcatacagaa gaaagttctt
    16741 gattaatgta gaagtttcta atattcttga ttatttcaaa tcaataaaaa agcct
//
LOCUS       DACCYH010000062        16512 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.063, whole genome shotgun sequence.
ACCESSION   DACCYH010000062 DACCYH010000000
VERSION     DACCYH010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.063"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..546
                     /locus_tag="IVY85_001950"
     CDS             <1..546
                     /locus_tag="IVY85_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAP8044963.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSLVQFRKTTVTVV"
     gene            735..1403
                     /locus_tag="IVY85_001951"
     CDS             735..1403
                     /locus_tag="IVY85_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP8044964.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1603..1833)
                     /locus_tag="IVY85_001952"
     CDS             complement(1603..1833)
                     /locus_tag="IVY85_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044965.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1960..3342)
                     /locus_tag="IVY85_001953"
     CDS             complement(1960..3342)
                     /locus_tag="IVY85_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP8044966.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(3498..4685)
                     /locus_tag="IVY85_001954"
     CDS             complement(3498..4685)
                     /locus_tag="IVY85_001954"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP8044967.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4692..5723)
                     /locus_tag="IVY85_001955"
     CDS             complement(4692..5723)
                     /locus_tag="IVY85_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8044968.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5769..7193)
                     /locus_tag="IVY85_001956"
     CDS             complement(5769..7193)
                     /locus_tag="IVY85_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8044969.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            7368..8273
                     /locus_tag="IVY85_001957"
     CDS             7368..8273
                     /locus_tag="IVY85_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8044970.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            8364..9041
                     /locus_tag="IVY85_001958"
     CDS             8364..9041
                     /locus_tag="IVY85_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8044971.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            9322..10218
                     /locus_tag="IVY85_001959"
     CDS             9322..10218
                     /locus_tag="IVY85_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8044972.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(10273..11643)
                     /locus_tag="IVY85_001960"
     CDS             complement(10273..11643)
                     /locus_tag="IVY85_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8044973.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11881..12627
                     /locus_tag="IVY85_001961"
     CDS             11881..12627
                     /locus_tag="IVY85_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8044974.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12624..13463
                     /locus_tag="IVY85_001962"
     CDS             12624..13463
                     /locus_tag="IVY85_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8044975.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            13464..14414
                     /locus_tag="IVY85_001963"
     CDS             13464..14414
                     /locus_tag="IVY85_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8044976.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14555..15307
                     /locus_tag="IVY85_001964"
     CDS             14555..15307
                     /locus_tag="IVY85_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8044977.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            15326..16477
                     /gene="queG"
                     /locus_tag="IVY85_001965"
     CDS             15326..16477
                     /gene="queG"
                     /locus_tag="IVY85_001965"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP8044978.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5371 a         3087 c         3320 g         4734 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtctc gtccaatttc gtaaaacaac cgtaacggtt
      541 gtgtaaaaat atcattttag agcataaaaa aaaggagtgg atttctccac tccaaaaaag
      601 tctaactttt gggggtcact acagtgtcac agtccttttt ttagtaaaag gatacatttc
      661 ttccttgtat attccatgcg tgttcgtgag aaagcatata taatttaatt aacagatagt
      721 tagggggaga aaaaatgatt ttttcaatta caatgaaact ggtgttcggg ttgatcggtt
      781 tattattagt ggtacgtttg cttgggaaga aatccatgtc agaaatcaca ccgtttgatt
      841 tagtctatac gcttgtttta ggtgggatct tagaagaatc aacctatgat gacaatgtcc
      901 atgtagggca tgtcctattt gccattgcgc tttgggcagt aatgatttat ggtatcgaac
      961 gtatcgtcca aaaaaatgaa aaggtcaatc gttgggtcaa aggcgagcca tctgttctaa
     1021 tcaaagacgg cgtcatcaac atgacagagt tgacaaataa ccatatcgag atggagcagt
     1081 tgcgagcaat cttaagacag caagaatgct tttttcttga aaatgccaaa catgttatcc
     1141 tagagaatgc cggtcaaatg agtgtattga aaaaatcaga tgaagacaaa gctttatcga
     1201 tcctgcttgt agatgaaggg caaattcagc ataaagtgct tcagtccaat caattgactg
     1261 aagcatggct tatggagaac ttgaagaaag aaggctatgc agatgtaaaa cagctgattt
     1321 atgttgaatg gtcagaagaa aaagggctct acatactaaa taaagaagac gtcatgcatg
     1381 aatcttaccg aatagatgga tagatagaca gaggttgtga aatcagcgcc ttggcttgtt
     1441 actcaaaact aagcgacttt ttcacaacct ctgatatcta atatacgttg ttccagaagt
     1501 tgcttcttcg aaaaataagc caaaattcca caaaaattta agaagcaatt ttcgaaaaat
     1561 cctttcttat tttttgaagt tgatcacttc tgtcacaacc tctcatgtca caaaccattt
     1621 ttttgtttcc tttaactcaa tataccgtac gtcttcccac aatatcggct gatggtcaat
     1681 cataaagtaa tcttcatagg cttcaccaat gaagtgccct tcgacctcat ccaagagtct
     1741 tccaaattca tcttttaaat tgagctgaat gattactgct ttacgagata aaaaggcttc
     1801 tgtcagtata ctatcgattt cttgcaatgt catttgttgt ttaggaagag gctgtttgcg
     1861 tgcttcttct tcattttttt gataccgttg actagttctt ccatcgcaaa agcagtcccc
     1921 cattttaatc caaatggccg atctcgatac tcgttatagt caatgaattt ttttgctgtc
     1981 cgtctcatct tttccacctt ctaatccact cataccacct gcatgcccgc caacgagaga
     2041 actacgtgcg atggcgcgtc ctccttgcaa gagactatgc gcatggatga ttgattgaaa
     2101 gccgtatttt tttctaatcg tatcgacaac aaaatcaact ttcgcttcat ttatctgctt
     2161 ttcaggatct tcaaaaaggt taagctgtaa acctgtcgcg tatacgaggt cggaacagtt
     2221 cacgcctaca cgccgaatat cctggtagtg gtaatgctgg tggaaaatgg tcaataaagc
     2281 atctgcaaga tctctgctag catttgtcgc ttcgattttc atttgctggt gaaacccggt
     2341 cttgccattt ggatcaagat agcctaaaga gtatcctacc cataagctga ctacttgtgt
     2401 ttttgctgca gatcgtctca agcgagtgcc gacttggtcg gccatttctt tgataacgat
     2461 ctcgatttct tctcgtcttg tataatctct atctaggatt tgactatttc cgatactttt
     2521 tgattttggt gtatattctt gtcccaaaaa acttcgatcg atgccccaag catgggcata
     2581 aagctgagta ccgatgaccc ctagcttgct tttcaagtga taataatccg cgtgagctaa
     2641 atcataaaca gaatgtatac ccatttgttt cagacgtttt gctgttcgtt tgccgattcc
     2701 ccaaaagtca gtgatatctg agattttcca taacttttct ggtacatctt cataacgcca
     2761 ttcagcaaac atttgctttc cgtcttttga gccattgtct aaggctagtt tagcaagtag
     2821 cgggttatcg ccaattccta tagtcgtgta gattccggtc tgctcaaaca catctttatg
     2881 gatcagttta gccatgtcgt acgcatttgt tttaccaaag agtgccatgc tatttgttac
     2941 atcaaggaag ctttcatcga cggaatagac atgatggttc gcttcgtctg catatttttt
     3001 atagatagaa ttgatttctc tgttcttttc catataatag gccatccttg gggcaacaat
     3061 atataaatca cttggatagg ggaagggaag atctctggca cggctgatat tggtgatgcc
     3121 gtaagctttt tttgctgcag gactggaagc caagattaat ccgctacccc gtgtttctgt
     3181 cgaatcggac ggataggaca tgactactaa tttagttgta aggggatcaa gatccttttc
     3241 aacgcactct acggaggcgt aaaaagattt gcagtcaata cataaaatat ctcttgaagg
     3301 ttctttttca taaacaaata ccggattttg attcgcccac ataacaatca cctcttcaac
     3361 acaattagaa cacacgttct ctttccgtgt caataaaaaa cagcaatttt caaatacgag
     3421 caaaaggctc acatttggaa attgctgaga ggttattttt atctatttgt ttttagctga
     3481 gatcgttgat ttttttatca aacatgaaca gcttctggat cgatctttgt tgtcccattt
     3541 ccaatgtgtg caacataaaa ttccggagca tagccgacaa ctttcatgta ttcatcatag
     3601 acattgtttt tgaaggcagg tatattttct tctttgacaa gagcaatcgc acagccacca
     3661 aatccagctc ctgtcattcg tgcacctaag acaccttctt gtttttgagc agcatctact
     3721 agtgtatcta attcgatccc agttacttca taatcatagc gaagagagtg gtgagaatca
     3781 tttaacagtt gaccaaatgc ttctaagttc ccagcttcta attcagcttt tgccttcaaa
     3841 gtccgctgat tttcagaaac ggcatggcga gctctgcgta tcaatgtatc atcgccgatt
     3901 tggtcaatag ttgattcaaa ttcttcttca tctaagtctc ctaaagaaga gataggcaaa
     3961 gttttttgca aacgtgccaa ggcttcttcg cattcgctac ggcgttcatt gtattttgaa
     4021 tctgccaatt cccgccgctt attcgtattc atgatgacga ttgcataacc atctagtttc
     4081 actggaacca tttcgtattt cagcgtattt gtatctaata agattgcatg atctagctca
     4141 ccgaagccga tggcaaactg atccatgata cctgaattga caccgatgaa ttcattttct
     4201 acttttttac ctgtttggac tagttccaag cgatctgttt ttaaatcaaa cagatcttct
     4261 aatacaacac ctactaataa ttctaatgaa gcgctactgg aaagtcctgc accattaggg
     4321 attgttcctt ccactaataa ctcaaaccca tgatcgattg agtgtccagc ttcttttaat
     4381 ttaaggacca ttccttttac gtaatttgcc cagtttgctt ttttatcata ttctagctca
     4441 tctaaagtaa aactaatgac accgatatcg tcaaagtttg tggaataaac taatatttta
     4501 ttatcgtctc gaggtgctgc tacaccttgc gttccataag tgatggaagc ggggaagaca
     4561 tggcctccat tgtaatctgt gtgttcacca atcaaattga tgcgtcctgg tgagaagtat
     4621 tgtgtagttt ttttaggtcc gaatgtttcg gcaaattttg tgtttaagct tcttttgatt
     4681 tccatgatga gttattcctt tcttgttttg attttgaatt ctgttactgt ttcaaatact
     4741 gatccggctt ttaaatgaat cgaaccaaat gctgagaagc gctctgcacc tggagctgtt
     4801 tgagtctcaa atgtgatgcc accgtgatgt gccagttttc ggttgcgcat ttctggtgtt
     4861 tctgtgccga aatttgcagt gaaaataacc acactagaag catctgtagc aatatctact
     4921 tggattcttt tatcggggct agtcaagcga gcggcttctt tacccaagcc ggtttctttt
     4981 aagaaaaatg gatgatcgat tccgtcaaca agattttttt gatccaaatc actagcaaaa
     5041 acggacgcta attttttagg tatttgaaaa tcgaaagcac ttcctgcagc attttctttt
     5101 acccctatag gaatactatc tgttcgcaaa ggagcataag cttcgctatt gagccaaagc
     5161 tcatgttgat cgattgactg agaagcatct cccgtcaagt tgaagtaaac atgatttgtc
     5221 ggattgaata aagtatcttg gtcactggtg ccgcgagagg tcactcgcca gatattatct
     5281 tttgtcaaag tatagcggat ttccacatcc attgtacctg gaaaaccatg ttctccgtcg
     5341 ggacttgtag tagtaaaaat gacagaagct tcattttcgc cattcaaaat gacataagac
     5401 cattttttta attcaaatcc aggagcacca ccatgcagat tatgtccagt ttttggatct
     5461 gtggctaatt ggtaagtttt tccattgaga gaaaagcggc cattttcgat tctccctgcg
     5521 gtgcgaccga tagaagcacc gatatacgga tctttttcga tatattcttc agcggtgtca
     5581 aatcccaaaa ctaattctcg atcatttgat tttaaactga cgattctagc acctaaatca
     5641 gagatagaaa gtgctagctt gtttttgttc tccaaggtta tcaagtgata accttggcca
     5701 aaatcttttt ctgtaatctt cattttcaag atcctttctt aatgggaagg aactactgca
     5761 tcgtgaagtt aatcttccat tgtatcagct ggagccaatt tgttttccaa tttctcaacg
     5821 atttgtgcat gttttttctc tgtcaatgta atcttccaag caaaaataat agcggagaaa
     5881 atcatcaaag cagcaggagc atagaatgca taagctttga aagtcgttac gccatgagca
     5941 gtgatatcag cagcagtagc attaccagtc atcccagaag cgatagcgac aaaccctacg
     6001 atcccgttag acaaagcacc agcgatttta tctagtaaag ggcgaattga tagagtaaca
     6061 gcttcgttac ggataccatt tttccattga ccatattcaa cagagtctgt gatcgtcatc
     6121 aaagcggata agaaaatcaa ttgttgaggg aagtagaaca atactagccc gatgataacg
     6181 attggcaaac ttgaacctgc gatcgtaaag aaaaagtaag aagaaatcat cattgcgatc
     6241 cctccaaggt aaacgtagcg acgagagatg acgcgtgtaa gcattgggaa tagtggaaca
     6301 gctactaaac caagtaaggc agcaactaga ccaacgatcc aaaattcagt tggttggcca
     6361 agtacatatt tgaagaagta gaataatacg gcagttgttg ctgcattggc aatcgcataa
     6421 gcgatataag caagggcaag ccacatcaac tgatcatttt gagcaactgc tttgaacaca
     6481 tctttgatcc cagctttttc attttgctga cggatcgcag tatcgacttc ttttgtgccc
     6541 aaggcagtga taatcgcagt accgcctgaa ataacagcag caatcacacc gaaccagaac
     6601 cagcctgaag cgccttcaat atgtttatga gtaacaagat aggtaaagaa agtaacgata
     6661 gggacaacga ctagagtagt accatttgca cctagagaag agccgaaacg agcaaacgta
     6721 gcaaattttt cacgttcctt tgtttccgta ctcaaagctg gaatcattga ccaaaaagcg
     6781 atatccttaa atgaataaaa actatccaat aagatgaaaa cgattgcaaa taatatgata
     6841 aataaagtcg tattatgagt agctaagcca aagaaattgg taaagattgc taccaagagt
     6901 acggaactaa caacgccgcc gataactaac catggtttga atttaccgta ttttgtacga
     6961 gtattatcga tgatgccacc aatcaaagga tcaaaaataa tttcaactaa gcggatacct
     7021 acaactaagc ttgtaactgt agcaatcatt tttgcattcg ttgccttatc tgttcctgcg
     7081 aacatctggc ttgtcacgaa taccataaaa taagtgctta atgtaacata gaatgtgtcg
     7141 tgtccaaaag cgcctaaagc gtaggacaca cgttggatca ttttgctttt cataatctgt
     7201 attcctctcc tgttcaatat ataatactaa gtaaaccggt tacaacgaat aacaaagatc
     7261 ttttaatgac ttaaacttaa caagaaatcg cttccttttt aatatatttc tgtgactttt
     7321 atttaacttt ttgttgcatt atactaaagg agtaaggagg gatagacgtg gagcaatcga
     7381 tttttttaaa aaaggaacat caacaagtag aaaaagaatt gtattttgat ttttgtggtt
     7441 tctctaaaac gatgccgttt cattcttttg gtcctgctat tcgcaatgat tacatcctgc
     7501 atgtcgtgat ggaaggcaaa ggatcttatc atgtgaaaaa tcaacaattc caattgaaaa
     7561 aaggtgatct gttcttgatt cgtccaggag actcgacgtt ttacttatca gatggagaag
     7621 atccgtggat gtattgctgg ctttcatttg gtgggagtgc tgcagaagaa atcattcggc
     7681 agtcgctttt cagagaagac caatacacga tggtctcttc tgacatacaa aaatatatcg
     7741 acattatttt agaatgcatg aaagatacgg aagaaacctt ggaaaatgaa ttacagttaa
     7801 cagcattaac ctaccgtttt ttatcagtcc ttttaaaaga tggcggaaag gtcagcggtg
     7861 gtgtacaaaa ggttcattcc tctttagctg ttgaaacagt caaatacatc gaggcacatt
     7921 atgctgaaaa attgtctgtt gaggaaatcg cccagcattt agcggtcaat cggagccatt
     7981 tgtcgcgagt attcaaaaat catatgggta ccagtatcaa ggaatatttg atcggggtac
     8041 ggatcaatcg aggggcgttt cttctttctc tgacggatga atccgtggaa agtattgcct
     8101 atcaagtagg gttcaatagt ctagtcgtat ttagccgaat gttcaaaaag agtaccggag
     8161 aaacggctac gaactatcgt aaacgtatga aaaacgaaaa ttttaagaat attgctttgg
     8221 ataccttgaa aaaacagttg gaaaaacaaa ccgcagtttc ttggacaacc tgattagtct
     8281 ggttaaaatg acaagcatcg gcaaaagaaa cttgtggcgg ttatgctaca ctgtaaagcg
     8341 aagactaaaa aggaggaaaa gggatgcgta cacttatcat cattagccat ccaacgatcg
     8401 aagaatcttt aaatcagcaa tttttcaaag aagcttctgc tggtttacca gctacttggc
     8461 atcatttgga atcaagttat ccagacggaa aaatagatat agagactgaa ctacagctct
     8521 tggcccagca tgatcggatc attttccagt ttccgtttta ctggtatagt tctcctgctc
     8581 atttaaaaat ttggcaagat aatgtcttgg aacgagcagc acatgtatta aaagggaaag
     8641 aactaggaat cgtattgaca acaggtgtgg cggaaaaaga ataccaagct ggaggaaaag
     8701 aagaatatac gatttccgaa tttttgaggc cgtatcagcg aatcgcaaat aaattccata
     8761 tgacctatct tccgccattc gttttagcac aatttatgta tttatcccag gaaaaacgtt
     8821 gggagaagct aattgcttat cagcagtatc tctcattgga aggcaaacca tcgctgactc
     8881 agcggattga ctggtttatt caaagagtac aggaaaatca gaaaatgcag gaagaagaat
     8941 ctgaaaaaca agcatatatc gtagaagcac tgactgaggc aaaagagcag atcgaagact
     9001 tgacctttac actgcaagaa atgaaaggaa cttcattatg acaaatgatt ggctgatcca
     9061 acaattagat gaattaagcg aaaaatcaga atatcaagac cgtgcgctct tatatgaaat
     9121 aaagaaactg atggctgaga tagacgaacg aacaatgcag ctgcaaggag aaatcgacgg
     9181 acaatcttgg aatcatctca actggtgaaa ttttcttata aataacaaag ataaaaatgt
     9241 gcacagcgac aattatttgc tacaataaga atatgaagcg tttgataacg ctattataca
     9301 taaaaattgg tggtgaaaaa aatgtatgat gaaaatgagt ggggagaaaa tgttcggatg
     9361 acccgaagaa gacagcaaaa acaggcagct cgtaagcgga tgatcatgat tggggcagca
     9421 gttgccgtgt ttttgatcat agcaggagga gttgcgggtc tatattttca tggaaaaaat
     9481 tcgtcagaag cgaaaagtac gaccataacg gaaacaacga caaagaaaac agagacgaaa
     9541 caaacaagtg agtcatcaac taaaacaaaa gagacacaaa cttctgaaga aaaaacagca
     9601 aatggtttgc cagagcaact tcaagcaatc gtaaatgatt attcgaaaaa gatcgaagaa
     9661 aagacgcctg cattgattga agaataccag acagaaattc agggaaatca agagggaata
     9721 gctggtttat cagcagttgc taaccaaaaa gcacgtgagc ttcaagcaat ctccgacgaa
     9781 gggatcagaa aattacgtgc agcgtatcag gcagcagaaa ataaagacgg tgtagactta
     9841 gatactttga tcaatcaatt atcggctaat tatacgaatc atgtcgctaa gatttccgat
     9901 atttatttgc agactagcgc ctctcttcaa gcagagtcaa caagcacaca agacacaaca
     9961 tcttctgatt ctgagacagc agagtcggta gaagcttctg aaatgcaagc tcgaacaaca
    10021 caagacacaa cagattcctc tgaaactaat accaacagcg cgacttcaca ggctgcgaca
    10081 accgtagttt tggaaggaga aggtccaaat caaatagccg aacgaactgg cgtaccagta
    10141 gaaacgatct tgtcgctgaa tgggatgagc atggagaatt atttcctgac tcccggtgag
    10201 acgctaaaat tgaattgata aaagcaataa aaaagaacga agcttccact aaggtgaagc
    10261 ttcgttcttt ttttattttc cggttctttt ttcgcgtaca aagtaatgga tcgtcatgta
    10321 aagcaagaaa acgagtccaa taatcaatga gattctcgtt tcaggattga taaacatgaa
    10381 aatcaatacg atagctaaaa aagcaagtgt cacataattc gtataaggag atagtggcat
    10441 tttaaaagga tgatcatgca ttttatctgc gtgcttcttt ttgaagcgaa tctcgctgat
    10501 caaaatcact acccaaggaa ccataccagg caaaatactt gagctgtata cgaaaacgaa
    10561 aatattttct gactggttga tgaacattgg tagaagataa tttaagatca gaccaattaa
    10621 gattccacct gagattgcta cgacaggata aaaaggcgca ccgtgacgag atagtttcag
    10681 aaatttttta gacatctttc catttatggc aagtgtatag accatacgac ttgcgctgaa
    10741 aatccctgaa ttgcaacctg ataatgccgc tgtaatcacg acaaaattaa tcagtcctgc
    10801 agcaaatgta atgccgactt tagaaaaagt ttgtacaaaa ggtgaaccaa tctcactcaa
    10861 ttgattccac ggatagatac tcacaatgat aaaaatagca ccaatataaa aaatcaagat
    10921 tcgaccgata gtggactgga cagcttcaat gatcgtcttt ttaggattct ctgcttcacc
    10981 agcagtcatt ccgatcagtt cgattccttg ataggaagca acaacgatcg ataatgcaaa
    11041 gaaaaatcct ttgacgcctc cagtgaagaa tccaccattt ttccaaagat ttgaaatccc
    11101 tactgcctga ccatgattac caaatccaaa taagattacc ccaagacctg caatgatcat
    11161 caaaatgatc gttatgactt tgatgatcga aaaccaaaat tctaattcgc caaacgcctt
    11221 gacagaaata agatttgcca gcataagaaa caagacgaca atgattccgg gaataattgc
    11281 cggcaaatca ggccaccaat aattcatata agatcctaca gcaatcactt cacttattcc
    11341 tacgacaata tattgaaaaa tattactcca agcggtcaag tagccagcca caggatggat
    11401 gtactcgctc gcgtaatttg caaaagaacc agtcgaagga tcaacgtaaa gcatttcgcc
    11461 taaagcacgc ataatcaaat acaaaaagaa tcctgcaatc gcataggctc ccatcactga
    11521 gggccctgtc caacgtatgg ttgaggaggc acccataaac agaccgacac cgatcgttcc
    11581 acctagtgcg atcatttgga tatgacgggc actaagactt cgagttaact tttcttcttc
    11641 catacaaaaa ctcctttcgt tgttgagtca acaagaaaaa ataatcaaac gtttcaacac
    11701 atgtttttca ttattaggtt agccttattg aactcttgat gtgattctac agaaaatttt
    11761 cataaaatgc gaggaaaatt cgaaaaaaac tttttcatca ttctacttga aaatgctttc
    11821 cattttagtt aaactatatt taaataaaaa gttaggtaaa cctaaaaagg agtgtaagat
    11881 ttggaacgta cggccattgt aattcaagac ctttcagccg cttatcaagg gaaaactgtt
    11941 ttgaaaacta tcaacttgac gataaatcca caaaaaatta ccggtattat cggaccaaac
    12001 ggtgcaggaa aatcaacatt tatgaaatct ttgctagagt tgataccagc tactactggt
    12061 aaagtcacct tttctggaca gtcggtaaac tcagtccgta aaaaaatagc ctacgtggaa
    12121 cagcgaagtg aattggatct ttcctttcca attgatgtat taggcgttgt acttttagga
    12181 acatatccat ctttacgaat tggacaaaga cctgggaaaa aagaaaaaga acgtgcaaga
    12241 caagctttga aaaaagtagg gatggaagaa tatgccaaaa gacagatcag cgaactatcg
    12301 ggtggacagc ttcagagagt ttttattgca agagctctag cccaaggagc agaatggatc
    12361 tttttagatg aaccattcgt agggattgat gcgttaagtg aacgaaagat ctttgacatc
    12421 ttgcaggaat tgaagaattc aggaaaaacg attttgatcg tccatcatga tcttcataaa
    12481 gtagaagaat atttcgatga ggttattctt ttaaataaac agctgatcgc ttccggtcca
    12541 gtacaagaag tctttacatc gaagaacctt caattggcct atggtgagat catcagacat
    12601 ttagtaaaag gaggagagaa aaaatgatcc aatcctttat tgatggatta atgaattatc
    12661 aattcctgca atacgctttg gtcacttcca tgcttgtagg acttgcttct ggagtcatcg
    12721 gttcttttat tattttacga gggatgtctt tgatgggaga cgctatctct cacgcagttc
    12781 ttccaggtgt agccatttct tatatgtttg gttttagtta catctttgga gccactgctt
    12841 ttgggatgct aacagcagca gtgattggtt tcgtgactca gcggagccga ttgaaaaatg
    12901 atacagcgat tggaattgtc tttagctcct tttttgcatt agggattatc ttgatttcat
    12961 atgcccagag tgcgacagat ttgtatcata ttttatttgg gaatgtctta gctgttaggg
    13021 aaagcgattt gttgctgaca gcactcgtta gtggaatcgt cttgatattt gtctttttct
    13081 tttataaaga attgaagatc acatcattcg atccgacaat ggcaaaagct tatgggctga
    13141 acacatcgct gatccattat cttttgatgt tctttttgac attagtggct gtagtcagtt
    13201 tacagacagt aggaacaatc ttggtgattg ccatgttgat cacaccagcc gccacggctt
    13261 acttgctaac gaaccattta ctgaaaatga tcattacagc tgcaggaatc ggtatgctaa
    13321 gtgcagttgt cggtgtgttt ttcagttata gttacaattg gccatcagga gctacgatcg
    13381 tgttagcatg taccgcattt tttatccttg cttttttatt ttctccaaca aaaggaattt
    13441 tatttcagaa aggaagcaga tagatgaaaa caaaaaaatc actcttttta atcttggcag
    13501 ttagtttctt ggtattagcc ggttgcggaa agcaggcttc tgaccaagcg gatgaaggaa
    13561 gtaaagaaaa gttgtcagtc gtggctacca attcgatctt ggcggacatg gcaaaagagg
    13621 tcggaacaga tcaaatagat atccacagta tcgtaccagt cggaacagat ccgcatgaat
    13681 atgaagtact accagaagac atcaaaaagg caagcgatgc agatgttatt ttatacaacg
    13741 gtttgaatct tgaaacaggt aacagctggt tcgataactt gatggaaacg gctaaaaaag
    13801 aagaaggaaa agattatttt gcagttagca aaaatgtaga acctctttat ttaactagcg
    13861 gtgaagaaca tacaaaagca gatccccatg cttggctaga cctatctaac ggaataaaat
    13921 atgtggagga aatcgcacgt atattctctg aaaaagatgc agaaaatgcg acactctata
    13981 aaaaaaatgc agaagcatat gtggaaaaac taaaagaatt agatacacaa gcgaaggaaa
    14041 gttttgcttc tatcgaagag aacaaaaaat tattagtaac aagtgaaggg gcattcaagt
    14101 atttttcacg agcatatgat ttgccagcag cttatatatg ggagatcaat acagaaagtc
    14161 aaggtacgcc tgatcaaatg aaagcgatca ttgatcagat aagagcaaaa gaagtaccgg
    14221 ttttattcgt ggaaaccagt gtagactcaa gaagcatgga acgggtagca aaagaaacgg
    14281 gtttgaaaat ctatgataaa ctgttcactg attccatagc aaaagaagga gaacaaggag
    14341 attcttacta tcagatgatg aaatggaata ttgaaacgat ccatgaaggt ttgtcacaaa
    14401 caaaagaaag ctgaaaagtg caaggtctag cttgaggagc agaccttgca ttttttataa
    14461 gagatatgaa aaaaaattat gctgtttttc gtcgtacaga tacctttttt gaagaaattc
    14521 cgttatagtt agagtaagga aggaaggtgt ggctatgaat tttacacgta aagataaata
    14581 tacgattaaa gaagaagtct ttcgattgat cagccagcag gaaaataccg acaaggatca
    14641 agaccttcaa tttcttgaag cagaattagc taaattattc atagcaaatc aaaatgagac
    14701 aacagaacca gatgaagagc tgtacatagg caaatggttg tttgatgaat ttcagccttt
    14761 agatgaaaat accggtatcg gttattttgt gttcctttct ccagtcggct tcacacctgg
    14821 cgtattagtt aaacaaaacg acgaaataac cgtcaaagca tggcctgcat tattttcagt
    14881 catcaaagca gaaggagtaa tcaaaaagat ccagtcggcc tattataaaa gtcaatttat
    14941 tgccaacagc aaattaatga atatgtttta taatcgtcta gtagaaattg ctaggcctgg
    15001 gaaaatcccg caaacattca cgtacacaca gcaaaaaaat ctctatgaag aagtattgta
    15061 ttcccgcgat ctgtctgagt tttcgccatg ggaagaggta aggctccatg aactgttaag
    15121 tacaaaacca gtttcattga atttttttaa tgcattagat aacaaaagaa aattaatttc
    15181 aacaaattat tgggaagtat ttgctgcaag aaaaaaatgg atggaagaaa agaagattgc
    15241 cgatcagcag atccagtttg tccagctgaa aaaagaaaaa ctatattatg ggttatacag
    15301 ccgttaacag caagcgagga agaccatgac attaaaagaa gaaatcatac aagaaagcaa
    15361 acgattagga attgacaaaa tcgggttcac cacggcggaa ccttttgatt cattaaaaga
    15421 atcgttggaa gaacaaaaag cagctggcca tacttctggc tttgaacatc caaatatcga
    15481 tgagcgtctt tatccagaaa tgacatttga tcagccgaaa tcgatcattt ccattgcact
    15541 tgcgtatcct actaggatcc atgaagaagt gcctagggat gaaaaacgcg ggcagtttgc
    15601 tcgtgcttct tgggggatcg attaccatga tatactcaga gaccgtttga accgattgat
    15661 cgaattcatc caaacaagag cagaaaaatg gcaaaaagaa gaggaatggc gtctagctcc
    15721 gcaagtagat actggagaac tgatcgatgt cgctgtagca cagcgagctg gtttaggatt
    15781 tattggacga aacggattgt tgatcactga agagttcggt tcatttgtct atcttggtga
    15841 gatcgttact aatattgtat ttgagcctga taaaccagga gaatttggtt gcggtgactg
    15901 tatgcgctgc gtgactgctt gtccgacaaa agcattgctt ggtgacggaa gaatgaatgc
    15961 ccagcgatgt ttgtcttatc aaactcagac aaaagggatg atgccggaag aatatcgtaa
    16021 aaaaatgcga aatgtcattt atggatgtga tatctgccag ttagtctgcc cttacaatca
    16081 aggaaaagat tttcattttc atgaagagat ggaaccaaaa gtagacgaaa tatatccaaa
    16141 attaaagcca atgctttcga tgtctaataa agacttcaaa cagcaatttg ggcatttagc
    16201 tggttcatgg cgtgggaaga aacctttgca gagaaacgca ttgattgctt tagctaatct
    16261 aggtgatcga acggcattgc ctgagatttc agaatgttta acagatgtcc gccctgtcat
    16321 cagaggaaca gcagcttggg ctattggacg tctaggaaca aaagaaccgg aaaaatggct
    16381 tgatgtatta gatgaaatat tagctaaaga aacagaagaa gtcgttataa acgaattgga
    16441 acatgcccag aaattattgc ggagaaagtt aaaataaaaa aatagttaac attattcaaa
    16501 gtttttgtcc ag
//
LOCUS       DACCYH010000063        16080 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.065, whole genome shotgun sequence.
ACCESSION   DACCYH010000063 DACCYH010000000
VERSION     DACCYH010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.065"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(94..1137)
                     /locus_tag="IVY85_001966"
     CDS             complement(94..1137)
                     /locus_tag="IVY85_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP8044979.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1514..2479)
                     /locus_tag="IVY85_001967"
     CDS             complement(1514..2479)
                     /locus_tag="IVY85_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP8044980.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            2713..3558
                     /locus_tag="IVY85_001968"
     CDS             2713..3558
                     /locus_tag="IVY85_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8044981.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            3749..4936
                     /locus_tag="IVY85_001969"
     CDS             3749..4936
                     /locus_tag="IVY85_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8044982.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            4942..5298
                     /locus_tag="IVY85_001970"
     CDS             4942..5298
                     /locus_tag="IVY85_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP8044983.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            5300..5638
                     /locus_tag="IVY85_001971"
     CDS             5300..5638
                     /locus_tag="IVY85_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP8044984.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            6033..7067
                     /locus_tag="IVY85_001972"
     CDS             6033..7067
                     /locus_tag="IVY85_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8044985.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            7060..7746
                     /locus_tag="IVY85_001973"
     CDS             7060..7746
                     /locus_tag="IVY85_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8044986.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            7770..8618
                     /locus_tag="IVY85_001974"
     CDS             7770..8618
                     /locus_tag="IVY85_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8044987.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            8815..9588
                     /gene="sufC"
                     /locus_tag="IVY85_001975"
     CDS             8815..9588
                     /gene="sufC"
                     /locus_tag="IVY85_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP8044988.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            9601..10887
                     /gene="sufD"
                     /locus_tag="IVY85_001976"
     CDS             9601..10887
                     /gene="sufD"
                     /locus_tag="IVY85_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP8044989.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            10884..12119
                     /locus_tag="IVY85_001977"
     CDS             10884..12119
                     /locus_tag="IVY85_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8044990.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            12106..12576
                     /locus_tag="IVY85_001978"
     CDS             12106..12576
                     /locus_tag="IVY85_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP8044991.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            12581..13972
                     /gene="sufB"
                     /locus_tag="IVY85_001979"
     CDS             12581..13972
                     /gene="sufB"
                     /locus_tag="IVY85_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP8044992.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(14070..15212)
                     /locus_tag="IVY85_001980"
     CDS             complement(14070..15212)
                     /locus_tag="IVY85_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8044993.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
     gene            complement(15263..16060)
                     /locus_tag="IVY85_001981"
     CDS             complement(15263..16060)
                     /locus_tag="IVY85_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8044994.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDISKYSSVED
                     FFYKITKNQFETEKFISDLSKKYNFPYEKNSIIAKFLTDNHDKFKNN"
BASE COUNT         5220 a         2964 c         3380 g         4516 t
ORIGIN      
        1 tgttgttgtt agaaggatat ctgcaaaatt aaaaacagtt gtgattcgga cattgttcca
       61 tgcgagaatc acaactgttt tttcatgttc attctaaact ttgatcagcc aaataaatag
      121 taaagccatc actgtttgga gaagcccgac agctaatcca taagataaga agcgtgtacc
      181 ttctttcatg aattttgcaa agtctaaacg cagaccaatt gccgctaaag cagtgatctc
      241 aaaccagctg ctgataaaat gtgccgattg gtcaataact acaggtaaag aaaacaagct
      301 gttgaccaaa caaaagatca caaaccctaa tacataccaa ggaagcggta attttcgttt
      361 tttctgcgta tcagttggcg tttcaaacag agctggttgt ttcttgtgca tgaatttttc
      421 aaacagatag acaacaacaa cgagcatcat aatacgtaga atcttaaata gcatggcgaa
      481 ttcgacaaca gaattgtcaa gtaagcttgc cccagcgact acttgaccaa cagattggag
      541 tgtaccaccc aaaagtgcac ttttttcaat gagatttcca ccaaacaact gtaatccaag
      601 aaaagggaga gtcaacatca tcacagtacc cagtagattg actagtgtga tgatctgtcc
      661 tttttcttcc tctttcgcat ggatggaagg ggcgattgaa gcaatagcag aagaaccaca
      721 aacagcgttt cctccagcca tcatcaaaga agaagtatcg ctgaattgca gttttttacc
      781 aatcaagtaa gcaccgatga tagtgactga catttgcagt aacacaaaaa caattccttt
      841 tacaccaatt tgagcaatcg tttgagctgt gacagttgca cctagtaaaa aaacagagaa
      901 ttctaatagt ttgctttcag caaattttgt gccgattgct agaacaggct gtttaaaaaa
      961 agtatttcct aataaaatac ccaataaaat agcaatagtc gctccaccta actgtggcag
     1021 ataaatagca agaagcctgc tgaaggcagc gatggcaaaa gaaagcagta aaccgggtaa
     1081 gatttcgcgg accgctgaat cctttacaga ttttattgga tgtgacaatg atttcatttg
     1141 atcggttcct ttcgtcaata aatactaaaa gcatactcga acatagtagc actaaaccag
     1201 tattttgaca aaagaacaag aggtcgtgac aaaagtcttg tacgacctct tgtttcgaat
     1261 aaacggtgtt ccagaagtag cttctacgga aataagccga aatttcacaa aaatttgaga
     1321 agcaattttc gtaaattcct tcttatttct tgaagctgac cacttctttt acgacctctt
     1381 ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaaccagta caaaaacatg
     1441 aacagctgtt tttgtttggc cgttcttatt acttgatgca gaacagcttt ttcacaacct
     1501 attgaatcga ttattaagcg atttttttag aaacaaattt tccatgtttg ctatgttctt
     1561 taattaagaa acgtacgtaa tgagcaagaa cgtagaagac aactacactt gcaaacagac
     1621 attccgcaac ttttaagaaa tccaatacgc tactatagat acctaaactg ttgaagtaat
     1681 gaacagtagt aaatatagct gttgcagaga taacaagcgg gaatgtaaag gctgcatagc
     1741 ttggataaaa acgaagcttg aacaaacgtg gaagcataga cagcacaacg aaatacaata
     1801 cttgagataa agcgaacaat gctacgacaa gataaaaatt aggttcagca aagtttttaa
     1861 gataacctgt taggcaaaga gaaccaggtg ctgcaacgat cgtaatcagc ggacgtgtcg
     1921 gatgagccat atttcttacg aaaaagacac gtttgatgat gatcggcaat aagaacgcat
     1981 agcagatcaa tgaaatccaa aagaaaattt ttccaataag tgggaagaag ttcccggcag
     2041 ttacagtgat gaccccaaat ccaacgtaaa tgataaacca gcttggataa attgcttcaa
     2101 ttgaaagatc agctttgata acatggtgat aattgaaata aaagacaagg atcacttgga
     2161 agataacagc gcacagccaa atgaatttga caattggtgc aatactttca tatgaaacaa
     2221 aataagtaca aataaccatg agtgacatcg taaaagttgg tgaaacggag gcgatgattg
     2281 gatcatgcaa cgtttgtttc gtgtgttcga ataagataaa caatttaaca aaaatgaata
     2341 tcataagaat catagagata ccgccagtta gattgcctaa agcaacatgg tgatagtctt
     2401 taaacagatt tccaagggaa gctaacccaa ggatcaaccc gcagatcggg atcggaataa
     2461 ttttcaaaaa gttcttcata tataatattc tcctttttat ttaatagatt ttagtaccaa
     2521 gatgaacgaa tacattgtaa aatgaatagg aatgtatgat tacgagcaag cagacagctg
     2581 attgtcttca tctaagttac tagttggatc agcaactgtt ttgttgtaca tttctattat
     2641 agatggtttt ttgtatattt aaatcaaata gtttttatag taattatcat aaaaagttat
     2701 ggaagtggtg ccatgtttaa gttattaacg acttttcgag cagtatatga aacaagaaat
     2761 ttttccagag cagcagaagt actgttttta tctcaaccag cagtctcgaa tcaaatcaaa
     2821 cagttggagc gggaattgaa tatcgatctt tttcaacgta acggaagaaa agagatgctt
     2881 ccaactaagc aagccgactt attatatgaa cggacactgg ttttgttaga agaatgggaa
     2941 gaaactcagc aaaaattgct caatgaacaa aatgaagaag aagtatgtcg gatcgttgct
     3001 tcccacacct ttgctgtcta tcttttgcct cagttgatga aacatctgat caaaaggttt
     3061 cctaaagtgc attttgagat cgaaatcacg aattctcatg aatcattaga acaagtagcg
     3121 aaacatgaat ccgattttgg ttttatcgaa aagccattag agacttccgg cgtacaaaga
     3181 tactctttga tggaagacca gctagtcatc gctggggacc caaaaagcaa attgtggctt
     3241 gtaagagaag caaactctgg tgtttttcat tacacgcagc gttattttga agagatgaat
     3301 atccaagata agaagatgat cgtgaaaagt aacgatgtaa ttgttgcgtt gctgaaggaa
     3361 ggaatcggtc gctcgatttt gtctaagcgc tctgttccag aaaatgtgcc aatgactccg
     3421 ttgaatttcc atcgatattt ttatttcatc caacgaacca atctggtttc tgatcagctg
     3481 ctagagattg ccgaagagat ccttcgatac aatgaaagca cagaaacttc tcacagcgag
     3541 gagaatctca gctggtagat aattgcatgt atcaaaaaaa gttctatgat acactaaaga
     3601 taagcagtag gcttttgtac tgcatttgct caagcaactg cacaaacgga aagttaattt
     3661 ttcccaaatg tctatggaaa gactttccat tatctaactg gaccgctata ataatcagca
     3721 gtatggtttt taagaaaagg gatgaaacat ggaaaaaaaa ttaaaagctt cgaacgataa
     3781 tcgtattgat tatggcgtga ttttaccagt ctttctgtta tgtttgattg gtctgctctc
     3841 attatatgta gcacttagcc atgatccgaa caatccagca gtcttgaaag gcgtagggat
     3901 gcaagttgtc tggtatttag ttggagcggc agcaatcgtt gtcatcatgc atttgaattc
     3961 caaatggatc tggaaactga ctccttacct ttatggtttg gggttagtgg taatgggact
     4021 cttgctcaaa ttctatgatc agaatttagc tgcttcaaca gggtctaaaa actggtttcg
     4081 attcggtgcg tttactttcc agccagcaga gttgatgaag attgcgtaca tcttgatgat
     4141 ggcactgatc gtaactaaac ataatacgca agtaaaagaa cggacgatga aatcggactt
     4201 ttggctgatt gggaagctct tgatcgtgac aataccggtt ttagcattga tcatggccca
     4261 agatgacttt ggaacgatgc ttgtctttct tgcgatcttt ggcgggattt tcctgatgtc
     4321 tggaatctct tggcgcatca tcgcacctgt agtaatcttg gcagtagtcg ttggagcagg
     4381 aacgatcttc ttggttacga ctgaaggcgg aagagacttg ctttataaag ttggattcaa
     4441 atcttatcag tttgcacgta ttgattcatg gctggaccct ttccatgata cgtcaggaat
     4501 gagttatcag ccggcccaag gacttttggc aattggtacc ggtggcttgt ttggtaaagg
     4561 attcaacgta agcaatatct atgtgcctgt tcgtgagtcc gatatgattt ttaccgttat
     4621 cggtgaaaac ttcggcttta tcggtggtgc attcgtcatt ttcttgtatt ttattttaat
     4681 ctatcgtatg attcgggtgt gtttcgatac gaataatgaa ttttacgcct atatcgcaag
     4741 cggtttgatc atgatgctgc tgttccatgt ctttgagaat attggagcaa atatcggatt
     4801 acttccttta acagggatcc cattgccttt cattagtcag ggaggatcct cgattttagg
     4861 gaacatgatc ggtatcgggt tgatattatc gatgagatac caaaatgaag cacctgcaag
     4921 atcttcaagg aggtaagaaa attgtacacc ttttactggt acccgaaatg ttcaacatgc
     4981 aaaaaagcca aagcgtggtt agaccagcat ggcgttagtt atcaaaccgt tgatatgatc
     5041 aaagaagtac cgaaggcctc aacgattgag acttggctag aaacgagtca agtaccaatc
     5101 cgtcgcctgt tcaacacaag cgggatgaaa tatcgggaac ttggactgaa agatcaagta
     5161 ggcgagttta cggcttcaaa agcgagccaa gtcttatcga cagatggtat gctgatcaag
     5221 cgaccactga tcttacgaga ggaccagctt gttgcaattg gattcaatga aaaaatttat
     5281 gaaggagaac tgatataaaa tggcaggcaa gcatttgaag aaaaaggaca acctttggat
     5341 cttatttaat ggaaaagaat actgtgttgg tttgacaaat gaagcgcaag aagaacttgg
     5401 agacatcacg tttgccaatc tgccaaaagc aggacaatcg ctaaaaaaag atgaacctct
     5461 gatcgaagtt gaagcggaaa aagcggtcaa tgaatttgcc agcccgctaa cgggtacagt
     5521 ttcttctgtt aatgataaaa tcggtgaaga tatcaatatc ttgaatgatt cagatgaaat
     5581 gaatgcgtgg atcttaagtt tcaaagatgt tgatccaaaa gaatttgacg aattataaaa
     5641 tcagaaaatc acttgaaaaa agttgacact gattcttcca gttgctataa tcataagcaa
     5701 tcctatatga aacgagaaag ctttgaaaag aagagtacat gtcacggacg tttgaagaga
     5761 gccctgtttg ctgaaaatgg gtaacgaaaa tgagatggaa gatggtctta gagcagagta
     5821 agcgagcgta agcaaactta ctacgggagt gcccgttaca gcgcgacagt atacggaaag
     5881 aagctaagtc caagtactgg aagaggctgt tattgcgaag taacggtaaa tcaaggtggt
     5941 aacacggaag ttcaagcttt cgtccttttg cttaaggcaa gagggcgaag gcttttttgt
     6001 ttgaaaaatt ataagagaaa gatgtgaatg gcatgtcatt aatccaacta acgaatgtca
     6061 aaaagacatt caatgggaaa aacggaaaca tccaagccgt caatgacgtt tcactaaacg
     6121 ttgaaaaagg ggatatttat ggaatcgtcg gctattctgg cgcaggaaaa agtacattag
     6181 tccgtctcct gaatggactt gagcttccca cgagcggcga agtgatcgta aacgatcaag
     6241 atattacaaa attaaaaaac aaagaattac gtacattccg aaagaaaatc ggtatgatct
     6301 tccagcattt caacctgtta tggtcaagaa ctgtacttga aaatatccag ctgccgctgg
     6361 aattagctgg cgtaccaaag agcaaacgta aagaacgggc agaagaactg ttgcgtttag
     6421 tcgggttaga aggacgcgga caagcatatc cttcacaact ttccggcgga cagaaacaac
     6481 gcgtcggtat cgcacgagca ttggcaaatg atcctgagat cctgctttgt gatgaggcta
     6541 ctagcgcatt ggacccgcaa acaacagaag aagtattaga cttattgtta gcgatcaata
     6601 agaaattgaa tttgaccatc gtcttgatca cacatgaaat gaatgtgatc cgcaaaatct
     6661 gcaacaaagt cgctgtgatg gagttgggga aagtcgtaga agaaggagac gtattgactg
     6721 tcttcagaca accgaaaaaa gaagtaacca aacgctttgt acaacaagac atcgaaccag
     6781 aagctgattc gacagaacta ttagctgaac tcatcaaaga aaatccaagc ggccgtttgg
     6841 caactttgac ctttagcgaa agaaatgcca acgagccagt catttcccaa gcgattcgac
     6901 gctataacat cgatgtcaac gttgtgtatg gaaagatcaa acaagtgaaa gaaggttcct
     6961 ttggttcatt gactgtcttg atgactggtg aaaccaaaga attagatcaa gcagaagtgt
     7021 tcatcaaaga acaaggagta ggaattgagg tgatccatcg tggataaaag cttttcagaa
     7081 acatatctag attttaaaca aatcaacccg gaaacgatga atcaggctat ccttgataca
     7141 ctatatatga cattaatctc aatggtctta gtgacgatca tcggattagt tttgggattg
     7201 atcctttact ctattggacg caaaaacaaa ccgtatgctc gaatcattta tggaatcgta
     7261 tcgatcatca gtaatatttt ccggtcaacg ccatttatga tcctgatggt tttgatcatt
     7321 ccatttacta aagcaatcgt aggcactatg ctaggtgcgc aagcagccat tccagctctt
     7381 gttttatccg cagcaccatt ttatgcccgt ctagtagaga ttgcttttcg tgaagtcagc
     7441 cctggtgtac tggaagcagc agatgcaatg ggagcaagtt actgggaaat catctggaag
     7501 gtcttgatac ctgaaagtat tcccgcattg atttctggat taacagttac aacgatctcg
     7561 atgatcggat ttactgcgat ggccggcgcg atcggcgcag gtggacttgg aggtcttgct
     7621 tggcaggaag gctaccagcg tggtaatcta acagtgacat tcgttgcgac tttaattatt
     7681 ttactaatcg tatttatcgt tcaaggaatc ggtgatttct taactaagaa aacagacaaa
     7741 agataatagg atataggggg atgtaggata tgaaaaagaa aatttttgga tttgcagcag
     7801 cgcttttgct gactgtagga ttagcagcat gcggtaacaa cagcgacagc aaagattcag
     7861 caaataaagc agacgacacg acattgaaag tcggcgcttc accgacaccg cacgcagaga
     7921 ttttggaaca tgtgaaacca ttattgaaag atgaaggaat cgatcttgaa atcgtcaaat
     7981 tcgatgacta tgtattaccg aaccaatctt tggaagaagg cgacattgat gccaattact
     8041 tccagcatat tccttatttg aacaaagaaa tcaaagaaaa aggatatgac tttgtcaatg
     8101 ccggtgctgt ccacatcgaa ccaatgggct tgtactctaa aaaagtcaaa gatgtttccg
     8161 aattaaaaga tggtgcaaca atcattactt caaattctga atcagactgg ggacgcatca
     8221 tcacaattct acaagatgct ggcttagtca aagtcaaaga tggagtagat ctggaaacag
     8281 caacattcga tgatattgaa gaaaacccta agaacttgaa gttcaaccat acgatcgacc
     8341 catcactttt agcttctacg taccaaaatg atgaaggtga cctagttgca atcaacgcaa
     8401 acttcgctta cggcgcaggg ttgaatccat tagaagatgc cgtattgctt gaaaaggaca
     8461 actctcctta cgtgaatatt attgcgactc gtaaaggtga tgaagacagt gacaaaatca
     8521 aaaaactgat tgaagtgctt caccgcaaag aagtccaaga atggatcaca gacaaatggg
     8581 gcggctctgt taaaccagta aaggctgatg caaaataagg tatggaacaa aaaagatgag
     8641 aaataagtct gtaatggctt atttctcatc ttttttatac ctattctaat ttagaaatca
     8701 aaattgttta ataataaata aaagatattt gattttagta aggaaggtca ttgcgcttca
     8761 ttctcaatta gtctaaaatg agtgagaacg aaagagaatt tgggggtata atgcatgtct
     8821 gtcttagaaa taaaaaatct acatgtgtcg atcgaggaaa aagaaatcct taaaggcgta
     8881 aacctaacaa tgaaaaccgg agaaatccat gccatcatgg gacctaacgg cacaggaaaa
     8941 tctaccttat ctgccgctat catgggcaat ccaaactatg aagtaacaga aggcgaaatt
     9001 ttgtttgacg ggaaaaatgt tttagacttg gaagtagatg aacgtgcgcg acttggctta
     9061 tttttagcta tgcaatatcc aagtgagata ccaggtatca cgaatgcgga attcatgcgt
     9121 gcagcgatca atgcgaaacg tgatgaaaac gacaaaattt ctgttatggc atttttgaaa
     9181 aagttagatg aaaaaatggc tttactaaat atgccagaag aaatggctga acgttatctg
     9241 aacgaaggtt tttctggcgg agaaaagaaa cggaatgaga tcctgcaatt gttgatgtta
     9301 gaacctacat ttgcaatttt agacgaaatc gattcaggtc tggatatcga tgctttgaaa
     9361 gtcgttgcta aaggagtaaa tgaaatgcgc ggagacaatt tcggtgcatt gatcatcacc
     9421 cactatcaac gattattgaa ctatatcaca cctgatgtcg ttcacatcat gatggaaggc
     9481 cgagtagtca tgacagggaa tgctgaccta gctaaacgtc tggaagcaga aggctacgct
     9541 ggtatcagta aagaattagg aatcgactat aaagaagaag aagcgtaagg gggagcaaga
     9601 atgaaagaaa aaaactggct cgattatctt gacgcggtaa atgatttttc tttaagtaaa
     9661 ggcgaacctg attggatgag aaccttccgt caagacgcac tagcgaaagc agatgaactg
     9721 ccattgccgc atatcgatcg ggtgaaattc catcgctggt cgttatttga tgtgaaagaa
     9781 acacaaacca tcagtgaaac aggcacgatt ccggcatttg atgcaatgaa agacaatcct
     9841 gtgcttgtcc aacaaggttc atggacgatt tttgaacaac tgcctgtgga attagcagaa
     9901 aaaggagtca tttttaccga cttgttcact gcaatgatcg aatatccaga actagtccaa
     9961 gaatattata tgaaaaaagc agtaaacatg aacgaagacc aattgactgc actccatgta
    10021 gcttttatga acagcggtat tttcctgtat gtgcctaaaa atgtcgtgat cgatgaacca
    10081 ctcgagtcct tattcattca agatggcgca tccgatgagc acttcttcaa gcatgtgttg
    10141 atcgtggcag atgaacatag tgaattttct tatctggaac gtttccagac aaccaaagaa
    10201 caagtagcaa aatcatcagg taatatcatc gtggaagtca tcgctaaagc tggttcaaaa
    10261 atcaaatatt cagccgtaga tcaattagga gaaaatatta cttcttatat gaatcgccgt
    10321 gggcacattt tacgagatgc ttctgtcgac tgggcaattg gtgtcatgaa tgacggtcat
    10381 gtgatcgctg actttgactc agatttagca ggagaaggtg cacacgcaga agtcaaaatc
    10441 gtggcaatca gctcgggcag acaaatccaa ggaatcgata cacgagtaac caataaagcc
    10501 cctcatacga tcgggcatat tttgcaacac ggagtgatcc gagaaaaagg aacattgacc
    10561 tttaatggta tcggccacat cttgaaaggg gcaaaaggtg cagatgccca acaagaaagt
    10621 cgtgtcttga tgctttctga taaagcacgt ggtgatgcga atcctattct tttgatcgat
    10681 gaaaacgaag taactgctgg acatgctgcc agtgtaggac gagttgatcc agaagaaatg
    10741 tactatctga tgagtagagg attgcataaa gaagaagcag agcgcttagt gattcgagga
    10801 ttcctcggtt ctgtcttaac agcaatccct gtagaacaag ttcggaaaga attagtcgaa
    10861 gtaatcgaag ggaagttgaa tggatgatcg atgctaaaaa gctacgcaat gattttccta
    10921 tcttatttca agacgtcaat gatgaacctt tagtttatct ggataatgca gcgacgaccc
    10981 aaaagccaaa acaagtactc caggcattag cgtactatta cgaacacgat aacgccaatg
    11041 ttcatcgcgg tgtccatacg ttagcagaac gcgcgaccca tgattatgaa caagcaagag
    11101 aaaaagtacg ggcgttcatc catgcaaaag aaacagcaga agtgctgttt actcgtggta
    11161 cgacgacaag cttgaattgg gtagccagaa ccttcggcga ccgctatatc aaagcaggag
    11221 acgagatagt gctctcttac atggaacatc attctaatgt tatcccgtgg cagcagttgg
    11281 caaaacgaac aggtgctgtc ttgaaatata tcgaactaac agaagaaggc ttcctggata
    11341 tggaggatgc agcgaaaaag attacggaca aaaccaaaat cgtctccatt gctcatgtat
    11401 ccaatgtctt aggtgtcttc aatcctgttc aagaattagc tgaacttgtc catgaaaacg
    11461 gcggggtcat cgtagtagat ggtgctcaat ctgccccgca catggccatt gatgtccaag
    11521 cactagactg tgatttcttt gctttcagcg gacacaaaat gtgcggacca acaggtattg
    11581 gtgttttata tggtaaacga aaatggttag aagaaatgga acctgttgaa tttggtggcg
    11641 aaatgatcga ttttgtcgaa ttatacgaca gtacatggaa agaactacct tggaaattcg
    11701 aagcagggac accaaatatc gccggcgcta tcgcattagg ccatgcaatc gattatttgc
    11761 aaaaactcgg tatggacaat atccatcgat atgaagaaga actagccgct tatgtgttgc
    11821 caaaattaca agcaattgat ggattgactg tttatggtcc acaagacccg aattttcgga
    11881 caggagtcgt tgcattcaat ttggatgggt tacatcccca tgatgtcgcg acagctttag
    11941 acatggaagg ggtagccgtc agagcaggtc atcattgtgc gcaaccgcta ttgaagtacc
    12001 ttgaagtatc agcgactgct cgcgcgagct tttatttcta caacacaacg caagacgccg
    12061 accgtttagt tgaagcaatc atggcaacaa aggagttttt ccaacatggg actatctaaa
    12121 ttagacaatt tataccgtca agtgatcttg gatcattctt cccatccgca tcatcgtgga
    12181 agcttgactg actcgacaag caagatcgaa ttgaacaacc ccacatgtgg agatgtgatc
    12241 gaattgcaag tcgatgtaaa agacggaatc atcaaaaata tcgcctttga cggcagtggc
    12301 tgctccatca gtacggcaag tgcctcgatg atgacagacg ccgtcatcgg aaaatcaata
    12361 gaagaagcag aacatttaac tgtagacttt ctacagcttg tccaaggaaa agaagtcaaa
    12421 gatcccgacc aattaggcga tgctgctatg cttggtggtg tagctaaatt tcccgctcgg
    12481 atcaaatgtg cgacacttgc ctggaaagca ctaggaaaag caatggaaaa cgatggtacc
    12541 gcagctttcc atcattacca caaggaagag gagtgagaac atgggcgtac cagaattaga
    12601 agaatataaa ttcgggtttc acgatgatgt cgaaccggtt ttcagtacag gagaaggctt
    12661 gacagaagaa gtgatccgtg agatgtcaag aatcaaaggc gaaccagaat ggatgcttga
    12721 attccgtctg aaatctctag aaacatttaa caaaatgcca atgcaaacat ggggccccga
    12781 cctttcagac atcgatttcg atgccatcaa atattaccaa aaaccaagtg acaaacccgc
    12841 tcgtgactgg gaagacgtac cagaaaaaat caaagataca tttgaaaaga ttggtatacc
    12901 agaagctgaa cgagcttatc tagccggtgc ttccgcacaa tatgaatcag aagtcgtgta
    12961 ccataatatg aaagatgaat tcgaaaagtt aggaatcgtc tttactgaca ctgactctgc
    13021 tttgaaagaa taccctgaat tattcaaaga gtacttttca aaacttgtac cgccaaccga
    13081 caacaaatta gcagcgctga attcagccgt atggtcaggt gggaccttta tttacgtgcc
    13141 aaaaggcgtc aaagtcgatg ttccattaca aacgtacttc cggatcaatg cggaaaacac
    13201 ggggcaattc gaacgtacgc tgatcattgt tgacgaagga gcaagcatcc actatgtcga
    13261 aggttgtacg gcacctactt attcaagtaa cagcctgcat gccgccatcg tagaaatctt
    13321 tacaagaaaa gatgcgtatt gccgttacac aacgatccaa aactggtcag acaatgtgta
    13381 caacttagta accaaacgag caaaagcata cgaaggcgca accgttgaat ggatcgacgg
    13441 aaacctaggt gcaaaaacaa cgatgaaata cccaagtgtt tacttagatg gaaaaggtgc
    13501 acgaggcacc atgctctcca tcgcctttgc aggagcaaac caaatccaag atacgggtgc
    13561 aaaaatgatc cacaatgcac caaatacatc tagctctatc gtctctaaat caatttcaaa
    13621 agacggcgga gaagtaaact atcgaggaca agtaaccttt gccaaaaaca gtgcgggttc
    13681 gatctctcat atcgaatgtg acacgatcat catggacgaa tggtcgaaat ccgatacgat
    13741 cccattcaat gagatccaca acagccaagt ctctttagaa cacgaagcca aagtctcaaa
    13801 aatctcagaa gaacaactct actatctgat gagccgaggc ctgaccgaat ctgaagcaac
    13861 tgaaatgatc gtcatgggct ttgttgaacc attcaccaaa gaacttccaa tggaatatgc
    13921 agtcgaactc aatcgattga tcagttatga gatggaaggg tctgtaggtt aataactatt
    13981 tcattgctcg aaacgttgat attaaagcgt ttcgagcgtt tttttatttg tattttaatg
    14041 gttggctacg tttttggcta cgtttggaat tattcgccca taaattttgc aaatcggtca
    14101 ccagttttct taacagattg tggtgtgaca tgagcataga tattcatggt agttttaata
    14161 tctttatgac ctaatcgttc ctgtacttct tttatcgacg caccactttc aaacaataag
    14221 ctacagtggg tatgcctaaa tccatgtgga gtaatttgag gctccatcgg tgtctttttg
    14281 taaatccagt ctaaccaatc gtttactact tgggggtagt acaacttgtt tgtgtttgta
    14341 gtgaaaagaa actgttttgg tgaattcgtg ttatagccat ataagatcat tattttcatt
    14401 tgttcttgtt gccaaatacg cagttgttta agtgtaaaat catcaagctg aatcgttctt
    14461 gatgaatttt tagttttagg tacttgtaga actacttgat taaactcgtc ttgagcgata
    14521 gttttaccaa ttgttattgt tttattgaaa tagtctatat cttcccattg aagcgccaat
    14581 gcttcgcttt tacgcattcc tgtataagct aataaacgga aaaaagtgta cagtttaact
    14641 gaattatttt ctttagtatt ctgtaaaaac agttgtaatt gatctttcgt gtaaaacttc
    14701 aagggttgtt cttcttcaat ttttcttgga agcaatgttt tagacatagg attatcctta
    14761 cagacttcca ttgctactcc aaatttaaat atttgagcag ttgtttttct caagaaacca
    14821 tattgtttta atgttttgtg ccattcattc acctgttttt ggcaatagct aacacttatt
    14881 ttatccaatc gtaattcccc aaaagcaggt aatatatggt ttttgcaaaa acgttgagca
    14941 acggcaacag aagacggttt tactcttgtt ctgtagttat caatccataa ttcatacaac
    15001 tcttgaaaag ttgtaacatt gattttaaac tcttgttttt ctttaaagtc tacaagtagt
    15061 ctattaagtg ctgtttgcgc ctcagctttt gatttgaatc ttctacgctt tgtacgtata
    15121 tcttttccag tcttgggatc tttccctaaa taagtttcaa atccccaata tttttcgcca
    15181 ttttgtaatt tataactaaa aaatgttgcc atttgattct ctccttactt cattattgca
    15241 ggtagagaag ttatttgaag aatcaattat ttttaaattt atcgtgatta tcagttaaaa
    15301 attttgcgat tatactattt ttctcatatg ggaaattgta ttttttacta agatctgaaa
    15361 taaatttttc tgtttcaaat tggtttttag taattttata aaagaaatcc tcgacagatg
    15421 aatactttga aatatcttta taggctaagt ccatttcgtc taatagctgt tttaaagaat
    15481 caatcattct ttctttttca ccgtatttga aatcaccatt tcttatcttg tcttgaagtt
    15541 tactgttggc tcttttgaaa aatagctctt tagagataga gtgtctttgg ttagcgtact
    15601 cttcaatcag aggattgata ttattagcaa gtatctcatt tagataaatt tcaaaatctt
    15661 ttgagtaata ttttgtaaat atatttttta ttataggatt caataactca tagtctttat
    15721 ctagcgaaaa cgtatcacta tatctttctt gaatgtcttt aaagactttg tgaggtattt
    15781 ctgggaagtc cttaatatat agctcatacc ctatatcgat aaggtatgaa gttatatatt
    15841 cttctaaagt tccccattta atccagtcaa ttgttgaatt agtgatagtt gataataatt
    15901 tcagcttttg tggatttggt aaattggtcc cacgttccca gttggatatg gtcgcacgtg
    15961 gtgaattatc tattttcctt ccaagttctt ccatagtaaa accacatgaa aggcgtaatt
    16021 gtttgattcg ttttcctgct tctgaaggat ttggcttcat cttttttctc ctttttattt
//
LOCUS       DACCYH010000064        15756 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.067, whole genome shotgun sequence.
ACCESSION   DACCYH010000064 DACCYH010000000
VERSION     DACCYH010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.067"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            25..2043
                     /locus_tag="IVY85_001982"
     CDS             25..2043
                     /locus_tag="IVY85_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8044995.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            2470..4884
                     /locus_tag="IVY85_001983"
     CDS             2470..4884
                     /locus_tag="IVY85_001983"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP8044996.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            5232..6785
                     /locus_tag="IVY85_001984"
     CDS             5232..6785
                     /locus_tag="IVY85_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8044997.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(7166..8950)
                     /locus_tag="IVY85_001985"
     CDS             complement(7166..8950)
                     /locus_tag="IVY85_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8044998.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(8965..10686)
                     /locus_tag="IVY85_001986"
     CDS             complement(8965..10686)
                     /locus_tag="IVY85_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8044999.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            10786..11547
                     /locus_tag="IVY85_001987"
     CDS             10786..11547
                     /locus_tag="IVY85_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8045000.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            12514..12782
                     /locus_tag="IVY85_001988"
                     /pseudo
     CDS             12514..12782
                     /locus_tag="IVY85_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            13454..>15756
                     /locus_tag="IVY85_001989"
     CDS             13454..>15756
                     /locus_tag="IVY85_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045001.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
BASE COUNT         5212 a         2750 c         3245 g         4549 t
ORIGIN      
        1 taaattgaag ttggaggata gaagatggaa aacattgcaa gaaaagaacg gagacgttta
       61 gaacagacca aacgttttcg aaaagcaaaa cgaggggcag caatcgtggg aactgcgatg
      121 gtaggctgtt ctgtcgctgc cccattagta cagccggttc ctgttcaagc ggatgaaaca
      181 cctgttcagt tcggcgcaag aatcaataca gcggcattta tcacagaaat tgctacttat
      241 gctcaaccaa tcgctcaagc caacgatcta tatgcatcag tgatgatcgc acaagcagtt
      301 gtagaaagcg gttggggcag tagtgcgtta tctcaagcac cttattataa tctttttggg
      361 ataaaaggca gctatcaagg acaaaccgtc tacatggata cgcttgaatt tttaaataat
      421 caatgggtaa ataaaaagga gcctttccga caatatcctt cttttgctga atctttcaat
      481 gacaatgcct atgtacttag aaatacttca tttggcaacg gttattacta tgccggtact
      541 tggaagagca acacgaaatc ctatacagat gcgacagctt gtttgacagg gcgttatgcg
      601 actgatcctg gttacgcagg caaattaaat aatatcatcg caacttacgg attgacccaa
      661 tatgatacac cagctagcgg aaacgcaggc ggtggagcga ctacaggtgg caataatact
      721 ggaagtgcag gtagtactgg cggttcaagc aattctggta atacaaactc aggttcagca
      781 acttcgacca cttatactgt ccaatctggt gattctgttt ggggaatctc aaataaattc
      841 ggtatcacaa tggatcaatt gatccaatgg aacaacatcc aaaacaattt catttatcca
      901 ggacaaaaac tgacaatcaa aggtagccag gaaaatggct cttcgacgaa taattccggt
      961 aacaatacca attcttcagg aaatgctggt acatcaaacg gaggacaaac aactggtgcg
     1021 aaatacactg ttcaatctgg tgattctgtt tggaagatct caaatgatca cggaattacg
     1081 atggctcaat tgattgaatg gaacaatatc aagaacaatt tcgtttatcc aggacaacag
     1141 ctaatcgtat caaatggcgg ttcttctaat gctggagcaa atactggctc aaatactggg
     1201 aatacaagtg gctctagttc gtccagctca agttcttcta atacagcttc aggaacgaaa
     1261 tatacagtta aagccggaga ctctgtttgg agcgtctcaa ataaatacgg aattacgatg
     1321 gctcagctaa tccaatggaa caatatccaa aataacttta tctacccagg acaacaactg
     1381 actgtttcaa acagtggatc aacaaacagc acgtcaggta atacaggttc gtcaaccaat
     1441 acaaataaca gcagttcagt taatactggc agcagttcgt ctcaaggcac ttataccgtg
     1501 aaagcaggtg aatctgtttg gagcgtggca aataaaaacg gcatcacgat ggatcagtta
     1561 atcgaatgga ataatatcaa aaacaacttc atttatccag gacagcaatt gatcgttaaa
     1621 ggtggaactt cggcaaatac aaatacaggt tcaactactt ctgcagcgaa accaaataca
     1681 ccgaatacgt ctgctacgac aagtacatca tccactggta atacgatgta taccgtgaaa
     1741 gcgggcgagt cagtttggag cgtagcgaac aaacatcata tcacgatgga tcagttaatc
     1801 gaatggaata atatcaaaaa taatttcatc tatccaggac agcaagtgat tgtgaagaaa
     1861 ggttcatctc aatcaacaaa ccaacaatca gttactggtt cgaaaaccta tactgtgaaa
     1921 tctggagaat ctgtttggag tgttgctgac agtcatggaa ttacaatggc tcaactaatc
     1981 gaatggaaca atattaagaa caactttatt tatccaggtc aaacgttgat tgtagctaaa
     2041 taaaatagct gttcgaagaa ataacgattg aattccacaa aaatatttgt tactataagg
     2101 ctacttaaat atgacaacac ttgaaaagga agtagtaaaa agactgaagt ttcagagagc
     2161 gcatggctgg tgagaatgtg cacggatgct tttgaactcg cctttgagtt gcccttgctg
     2221 aatacccgag taagcaagcg gcggtgacgg ccgttacaac gttcgaggcc ttttcactaa
     2281 ggtaaaatta ggtggtaccg cgtagattac gtcctataaa aagagcaatc tttttatagg
     2341 atttttttat ttcttttgtg aagtttgaga aaacaaagca aaaggaaata aaaataaaca
     2401 gattctgccg gttttttcgt tatatttata atagaaacaa gtgttttcaa tagaacagga
     2461 ggacagcaaa tgagttacaa tcataaagaa attgagaaaa aatggcaaaa atactgggca
     2521 aaaacgaata ctttcaacac ccatgatgat cctgaaaagc caaaatttta cgcactggat
     2581 atgtttccct atccatccgg tcaagggcta catgtagggc atcctgaagg ttatacggca
     2641 acggatatcc tttcacgtgt gaaaagaagt caaggataca acgtgctgca tccaatgggg
     2701 tgggatgcat tcggtctgcc tgctgaacaa tatgcgctag atacaggaaa cgatccagct
     2761 gagtttaccc aaaaaaatat cgaaacattc cgacgccaaa tcaattcttt aggatttagt
     2821 tatgactgga atcgcgaagt gaatacaact gatccggagt attataaatg gacacaatgg
     2881 atctttacga agctttatga aaaaggtcta gcttatgaag cagaagtagc ggtaaactgg
     2941 gtacctgaat taggaacagt tatttcaaat gaagaagtga tcgatgggaa aagtgaacgt
     3001 ggtggctatg atgtagtcag aaaaccaatg cgccaatgga tgctgaaaat cactgcttat
     3061 gctgatcgct tattggatga tctagaatta gttgattggc cagaaagcat taaagaaatg
     3121 caaagaaatt ggatcggtcg ttcagtagga gcaaatattg aatttaaagt ggctggtacg
     3181 gataaatcct ataccgtatt tacgacacga ccagatactc tattcggtgc aacttactcg
     3241 gttcttgcac ctgaactaga tcttgttaga gaaattacga cccctgaaca aaaagctgct
     3301 gtagaagctt atatcgaaga gacagctaaa aaatcagatc taaagagaac ggatcttgca
     3361 aaagaaaaaa caggagtatt tactggtgct tatgcaatca atccagttaa tggcaaggaa
     3421 atcccaatct ggatcgccga ttatgtatta tcttcatacg gcacaggagc catcatggct
     3481 gttccagcac atgatgaacg tgactttgaa ttcgctcaaa ctttcggact agaaattctt
     3541 ccagttatcg aaggtggaga tgttgaaaaa gctgcttaca cagaagatgg tacgcacatc
     3601 aactctgaat ttctagacgg catgaacaaa caagaagcaa tcgataaaat gaatgaatgg
     3661 ctagaagaaa atggcgtagg gaaaaaagaa gtcagctatc gcttacgtga ctggctattc
     3721 tcccgtcaac gttactgggg tgaaccaatc ccaatcatcc attgggagga cggaacagtc
     3781 acagctgtcc cagaagaaga gctaccatta cggttgccta agacaaaaaa catcaagcca
     3841 agcggtactg gagaatctcc attagcaaat atcgaggagt gggtcaatgt cgttgatcct
     3901 gtaacaggta aaaaaggccg tcgcgaaacg aatacaatgc cacaatgggc aggaagctca
     3961 tggtattacc tgcgttatat cgaccctcat aacaaaaaag aactagcaga ttatgaaaag
     4021 ttgaagcgtt ggctaccagt tgacatctac attggtggag cagaacatgc ggtacttcat
     4081 ttactatatg cacgattctg gcataaattc ttatacgata tcggtgttgt tcctacaaaa
     4141 gaaccattcc aaaaactata caatcaagga atgatcttag gcgaaaacaa cgaaaaaatg
     4201 tcaaaatctc gtggtaatgt agtcaatcct gatgatgtgg tcgaaactta cggtgcagat
     4261 acactgcgca tgtacgaaat gttcatgggg ccattagatg cttcgattgc ttggagcgaa
     4321 aatggcttag aaggaagccg taaattcttg gatcgtgtat ggcgtttgat cgttgatgag
     4381 aacaataaaa tgcgtgaccg tatcactaca ttgaatgatg gcaagcttga taaagtgtat
     4441 catcaaacag tgaaaaaagt gacagaagac tatgaaaatc ttcatttcaa tacagcaatc
     4501 tcccaattga tggtctttat caatgaagcc tataaagtgg acgctctgcc ttatgaatat
     4561 atcgaaggat ttgttcaact actcgcaccg attgctcctc acatgggtga agaactatgg
     4621 gcaatccttg gaaatgatgg tggtatttct tacgcaccat ggccaacata cgatgaagca
     4681 gcacttgttg aagacgaagt agaagttgtt ttccaagtaa acggtaaagt tcgtgcgaaa
     4741 agcaatgttc cccgagactt aggaaaagat gagctagaaa aagcagcatt agccaatgaa
     4801 atagtgcaag aatatattga aggcaaaaca gtcagaaaag ttattgcggt accaaacaag
     4861 ttagtcaata tcgttgctaa ttaattggct ctttgtcaat aaggactgat gagttgtgca
     4921 aaatcaaatc tgggccagaa tgaacttcat tctgtctcag attttttgtt atttcaaatt
     4981 gattaattga ttgatcaaaa aggttttgat tttcatgttc tcaaacacgt tcaatgtctt
     5041 tttcttctga atggtcttgt aagagatctc tgtaatcatc aaatttttgt ctattaatct
     5101 tagtattttt tgatgtataa actacctgaa attgagagta cataccaaat tcataaattt
     5161 tttattagtg ggactagtga ttgtatggat tttgacgcat aatgaatgaa gtatgagaaa
     5221 aggagagaca aatggatcaa gaactattta ttatcgaagt attatcagct aaacaaaaaa
     5281 ttatcgagaa agcgaaaaaa agagcgaaaa gaataatgtt caagctttta agcttagtat
     5341 ttttctctgg tttgcttttt ttcggagctg tatcgttcct atatcgccat atgagtgcag
     5401 tagtccgttc agctgaaggg atgcaaaaat taaataattt gtatcacttt tcgtggggag
     5461 ttggcggaat aacaattgtt gtgcttattg tttttcttct acttatttgg ctccgttacc
     5521 atcaattgtc gcgtcatttc gatcaatggc ttagtcaaag agcacaacag tttttacata
     5581 ataaaccaac ttctcaagat gatgactatc tttattttga gaagacttcg acgagtcaag
     5641 cttttcggat acaaaaaagt gattgccagt tattgctgag atcacttgat gatttaccta
     5701 tctacttagg cgaaagcaaa tcaaaattcg gaatgaacac attccatatt tttttactag
     5761 aaaacgaacc cagatgggaa aaaagccagt cttcgcctgg tggatacctg acaactaaga
     5821 tgagtacatt agtcactggt tttttcctca ttcttttagc tggaacaatc tattatggga
     5881 gatacactca gccaaaaatg tcttctcata taaatgctgc ttctcacatg acaatgaact
     5941 caactaacga tacgcatata caaaagacaa atgaagggtt gttagctgat cctaagcaaa
     6001 aggtaaattt gacaacacaa aagataaatg atctcaaact tgatacagag acgcactctc
     6061 tctatatgac aacagatagt ggtgctaatt gggtatttgt gccgattgat tcgcaatggt
     6121 tgcgttttgg cgaatacacc ctgacaacgg ggctgattcc tgaaggttat tggatggaca
     6181 acacatatga tatttccgcc gatttctcat ggtttatcta ttcttctggg gatgatgtac
     6241 gtctgctgac ttcaagagat aatggtaaaa catggcaaac gtctattatc acccaatcag
     6301 ttcagccgat acgttatcga aaagcggtat tttatggcaa cgatggtcta tttatctgta
     6361 catcagccag tgggatgtct gcagaaactt tgtacattta cactactatg gatggcggaa
     6421 tcacttggat aaaaagtgga agtacaatga tcaatcagcc agtacaaaat gtcagcttcg
     6481 ttacaacttc tttaggtttt gtttcgacac gtgaaagttt gttttataca aacaacggag
     6541 gacgctcatt caaagaatca gttctgtcga ttccggaaaa ctatcagaca ggggggatag
     6601 atctttttca atcaccaaat gaagttactc aaataagcgc gaatacgtta gagacaaaat
     6661 tttatttgtt gaaaaaagga ccaatcgatg cagggaaaat gtttgcctgc ctcttccgtt
     6721 caactgataa cggggaaaca tggcaattcg ttcagcaact atcgcaagta gaacaaggag
     6781 attaaagaag agagattgtg agcctgacgt tcagcaccga gtcacaagaa acaaattcaa
     6841 aaaacagctt ctcatatttg ttgaatagtg taactcgtga ttcggagttg acaagagcaa
     6901 caagaagcga ttttgaagta tagcttctcg tattttacaa gatcgtgtct tattgccgat
     6961 aggttgtctc ttgaacaccg tttattcgta aaaagggatt gtgacaagtc ttttgtcact
     7021 acttaccgaa cgaaactgaa ggagctatcc aaaaagtatc attagtatta ttctttgtgt
     7081 ttctagtggc accactagta catttaaaaa agctatcgat catggactaa tcatacatga
     7141 tcgatagctt tttagataat gtttgttacg ctatctgaaa ttgactatta taaagactgg
     7201 cataaagcgt tggtgattgg agtagttcag aatgtgttcc tttttcgacg atatcgccat
     7261 tcttcattac aaggatcatg tctgcatttt caatcgttga caaacggtgt gcaatcacaa
     7321 aacttgtcct atcttttgtc acagcttcca ttgcttgctg aatcaatgct tctgttcgtg
     7381 tatctacgct agaagttgct tcatctaaaa taacgacagg aggattcgct agaatgactc
     7441 gagcaattgt caataattgt tgctgtcctt gagataacgt gccgttttcg ctagaaatga
     7501 tcgtatcata tccgtctggc agtgttcgaa taaagtgatc acattgtgcg atttcagccg
     7561 cttcgataat ttgttctctt gtggcatcca ttttcccgta ggcgatatta tcagctaccg
     7621 ttccttcaaa cagccaggta ttttgtaaaa ccatcccaaa agaacgacgt agttctccac
     7681 gtgaaagctt ggtaatatca agaccgtcga attcgatctt tcctttattc agttcataaa
     7741 aacgcatcag cagattgact aaagtcgttt ttcctgcacc agttggacca acgatcgcta
     7801 ccgtttgttt tggctttact gaaaaatcga cttgattcat caatatttta tctgatgtgt
     7861 aaccaaactg tacatttttg aaagctattt ttcctttcgg ttgttctaaa agtcttgcat
     7921 ctggaaggtc tggaatttca tctgtttcat ctaatatctc aaatatccta tcgattgctg
     7981 ccattgcgga ttggatcgag ttgatcacat aagaagcggt agaaattggt tcagaaatct
     8041 gattgatgta ctgaagataa gcttgtaaga agccgatcgt cacaccacct gatagaacaa
     8101 gcatagcacc gaataccgca cttatgatga aggcgagttg attaatgaaa cgaatggccg
     8161 gatagatagc acagttcata aattgagcac gtttaaaagc gttgtattgc tttttattta
     8221 cttcgtgtat agcagcttct gcatgagctt gctgattgaa tgttttgacc actagattac
     8281 cagctaagat ttcttccatt cgattattta aaacacccag ttcatactga ttctgttcag
     8341 aaatctgttt gtttttgttc gcaatcttag ttgtaacaat tgaactgcca acgatcagca
     8401 ataagacgag caaagtaagc ttgctttcga tataaaacag catcagacca gcagcagcta
     8461 ttgttacgac agaagtaaaa aattgattga tacctgtcaa taacacttga gaaagttgat
     8521 tgagaccggt agttgtccga ctaatgatgt cacctacttg atgattgtca aaaaacgaca
     8581 tcggtaatgt tttgaatttt tttgtgattt cttttcgcac cctcaatgtg atcttttcgc
     8641 ttaacgctgc cattgtccgt tcctgaataa atgaagtgat gctgcttaag actgaacagc
     8701 cgatcagcaa taagacaggg aacaacaatg cttgtttgat atcactgaaa gaaagctgtc
     8761 caatacctgt ttttcttatt tctgataaga gatcatcgat cccgattccc atgatcaagg
     8821 gcatggctac gactaatagg ttcccgaata aactgcatag aaacaatccg taaaaatatt
     8881 tatgttcagg tttgatcatt ttccaaaaac gtatcaaagc acgcgtgttc aacgtttttt
     8941 tcttttccat cgtatccctt ctttctatat tccttgtgat tttgcaaact cctgataagt
     9001 ttggcaatta gacagtagtt cttcatgagt accttgtcca acgatttttc cttcatctaa
     9061 aacaatgatg ttatctgctt gttggattgt actgaggcgt tgagcaacaa tcacgagagt
     9121 tttctccgac atgtgtttat gaagggctgc tcttaatgct gcatctgttt tatagtctaa
     9181 agcggagaaa ctatcatcga atacatagat ctctgctggt ttgatcaacg ctcgagcaat
     9241 acatatccgc tgtttctggc cccctgaata attagaaccg ccttgagcaa caaagctgtc
     9301 caatccgtca ggaagggtgt ccactagttc ttctaattga gcgatcttga tcgctttaga
     9361 caactcttct gttgaggcat attcatttcc catctgcaag ttgcttaaaa ttgtcccgct
     9421 gaataagaaa gccttctgag ggacataact gatgtgggag cggatctgtt cttgagtcat
     9481 ttgagtaaga ggaataccac caagggttac ttttcctatc gtagggtcac taagtctcaa
     9541 gagaagtttc gccaaactac tttttccaga tccggtcccg ccaacgatcg ctgttgtttt
     9601 tcctttaggt attgtgaaat caatatcttc tagaactgga tcagagattt cagtatattt
     9661 aaatgtgaca tgttcaaaag cagctgcatt tgtctgatca aaggttggtt cagctactgt
     9721 tttgatcgtc tcttcctcaa tttcgatctc tgcttgcagt atttcttcta tccgtcgtaa
     9781 tgaggcaaac gatttaggga taatgaccaa taccatagct gcgatcatca agtaagcaag
     9841 tgtcagcata gagtattcga ctacagcagt cacgacaccg atttgtaatg ctccttctgc
     9901 tacaagattt ccgccaaacc acaaaataga agcataaacg attcccatga gcaaaaaagc
     9961 aattggagtg acccaagcaa atattcggtt cactttgatc atttgattag catagtcagc
    10021 aaagctctta ttcgttcgct tttcttcata tgattgatta tcaaaggcac ggatcatatt
    10081 aatcccagta aagaattctc tcaatgtgat ggtgatccga tccatttttg gctggatact
    10141 caaagataaa ggtgcccctc gtttcatcag tcgataaaca atccatacat aaaaaacaac
    10201 agagatcaat gtaacccatg ttagtaatag agaataacga aacatcatga gaatggtgaa
    10261 aacgcaaata accggtgctg gtaagatcat ttggcaaaat aaaacaatca tttgctggac
    10321 attatcgaca tcattcgtca ttcttgtcaa aagagatcca gaactaaaac gactgatatc
    10381 cttcattgac agctgttgga atttacggaa atatttctcc cgcgtttgga atccaaacct
    10441 agcggaagcc aatgctgaaa agtaactaga ggcaactgca gcgattgttc ctagaaacgc
    10501 tacaagcagc atttcaactc caattttttt tataactgag gcttcgccac ttgctatacc
    10561 tgtgtcgatc agctttgcta ctaattttgg tacacctaat gtaccggcga tttctaaaca
    10621 gatcatcaca aaggtaagga gagcgagtcc tttgtttttc tgtaagaatg tttttaagat
    10681 agacatatta ttcctccttt tttcctgata cttttcgatt ctataccttc aagtaacttg
    10741 aaggtcaata gaaaaaagaa taaactgaaa aagaggtgag aaaaaatgaa aaaaatgtat
    10801 acgattggcc aagtatcgga actatttgat ttgccaaaat caacgatccg ctattgggat
    10861 gaacaaggat taatccgttc aagtagacaa gaagagaatg attatcgatt gtttgatatc
    10921 gatgatattt ttatgcttta tgatatcgtt ttttatagaa aattagatat accgatcaaa
    10981 cagatgaaga atttatatgg aaaaacattg acggagttat atgagacact tgatgaaaca
    11041 gaaagaagaa tccataaaga actggtggtg atgaaacaaa aacaaaaaga gataagggag
    11101 caaaaaaaac agttgaaatt gatgatcgat acaaacgagg aagaatttcc tgtagaagag
    11161 atcccttttg actgtatgat ctcaacggag tttgaagata ttgtggaaat caaaaaattt
    11221 ctacctaact attcaagctt tgggatgatg agtatgccag cttccagttc acagacgatg
    11281 tatggatttt ttatcgaccc tagtgaagta catttattta ctcaagacgt gatttgggag
    11341 aaaaaagata cggctgttta tcgtcgattc ttattgaaat ccgagatgaa ccatgcggaa
    11401 cgtaataata ttttagaaat cagagaaaga atgtatgaaa aaggatggaa aacaggagaa
    11461 gtgatcggcc agtatctatt gacgaatacg gatgaaaaca atatcagaac tgaatattat
    11521 catgcatgga tagaaatgaa aaaataggaa atatgtgttt aagataactc gaaaatgatg
    11581 gttgttttag ttataaagaa aaattttctg gttcgattac gggtttaatg tgggggatta
    11641 ttagtcgtgg atagatagaa ttttagtttt ttagaggaaa tatgtcttta aaaatggaat
    11701 ggttgcaatt cttagcgata tatacgatgt aattgtcctc ttaaaaaagt atggaaaaat
    11761 aattgtattg ggtgttatgt ataattcatg gtgaactgag actaatgaga agaaaaattc
    11821 atattgttat gagtgagatt gtatcagatg agaatgataa attaaatagg tgcataaaag
    11881 cattacactt aaaatacatt gttgcttaca ttattacaat atgttaataa ggcaaaaata
    11941 aatagatgga ttgaataaac atatttttta atcaaatagt ccctactgtg actttcctca
    12001 gctatcggtg atactaccga tagcattttt ttgcttataa aataattata agtatttaga
    12061 gatttttctt ctaacattct tcaaacaaga aaaatcacaa aggttattgt tatttttata
    12121 tattcttttt aagaatgatt gtttttttat agaaatttta aaaaataaaa cacgttattg
    12181 atggatagtt agtttgttat ttggtaatat tatagaagtt gactggtagt gatttatttt
    12241 tcgtcgtttt ataaaaatgt tattttttgg aaaaattcac aaatatgtaa cgaatgtttt
    12301 tctggaattt gctgtgttaa aaagcagtat gactaatata aatactattg tcatttatgc
    12361 aattttttat ggatagtaat tttagagggg aatcaatgaa ggaagaatag aagggaaaaa
    12421 tagttgcaaa tacgccagtt agcaaaaata gcaatacatc ttcaagcttt aaaattcgag
    12481 agaatcatct tggtagtgga atcaactaca acaaaaggat taaaccaatt aatagataga
    12541 attaccaatg gaaaaataga aaaggtagtt attctataca aaagttgttt aattcgcttc
    12601 ggttatgaac ttagtgaaac cctttgcaat atatggtaca gaatatagaa attattgaca
    12661 ttacagaaaa tgatgaagat ttaatacaga ttgtcactat ttgtagatac cttttacaag
    12721 gaaaccgagc caataaagct aagaagatga ttaaggagtt gatggaagac gatacaagtt
    12781 aagaaagtga aactacgccc aaataaagag caaaaagagt aattggacgt ggaaaatgca
    12841 agaaatgaac catcgattcg gtggacagtt tattagtaat gacggtttga gaacatccat
    12901 gacgagaatg aaggaacatc ctaaatgcgt ttagttgaac caagtgccaa ataatatggc
    12961 caaataagcg atcagttatc gatgaatccc aagtattcta aactatatat tcattttgat
    13021 gctaagcatt gatatatttt tttgggattg atgaaggtta accaatacag ggactcactg
    13081 atgaaattct agagtctgac cttggtatca acgatttagt gattgtttct aatggaaaag
    13141 tataaaagta tataagtaca ttaacaaaat aactattagg gttacgctcc agcttgtgca
    13201 aggcactggc aggaggaatc gtgaaatcat aattatggtt aagccacacg gacaactaaa
    13261 agtcatacaa agctatcgaa tcaatcaaca gaagacaatc ggcaatcatt agctttgttt
    13321 acagcttaca ttgcaacgct tgagttgaac cgaaaatacg agggaggtaa cgcagccatt
    13381 ttgagttggg gaatcttttt agaaaattat cgggtacctt gttgtttagt caaaattata
    13441 agggggagta atagtgagga acaaatttca taaactgtta agtatgtttc tagttatgct
    13501 tattgtgatc aatcaatttg ggaacataac ggttctagct gagtctttag cagaacagcc
    13561 agagcgttcg ctgacgatcg aagttgatca agaggaggct gctttgggtg agcagttcca
    13621 gttgcgagtc agaggaacac cagacctgct aaatgcattg gtcattgata agagtgagga
    13681 tttgattgaa ggggaagtga agcaactcaa tgattacgaa cgtctgatta cttattcttc
    13741 ccagcattca ggtaaatacg aattgaccgc aagaagcggt gatattcaat caaatacggt
    13801 ctcaatagaa atcagtgtac cagaagaaag cacaagtgag tcttttaatg taccagaaga
    13861 aagcacaagt gaatctttta atataccaga agaaagctta agtgaatctt ttaatgaagc
    13921 cagatcatct gagccatcta taacgcagga atctcagatt gaaagacaag aggagctctt
    13981 gaattatgac atcggcatct ctgctgagcg ccccactttg ccgagcaggc atacacaaac
    14041 gttaaaggga tacattcaag gaggcgctgg cgatattctt gaagaatatg gggagatggt
    14101 cgtacgagtt cctagagcat cgattggctt aattgagttc attagagtgg aggtcgctga
    14161 tgattcacct ttaaagccac tggcatttta ccaagaaggc acagacgttg tttttccatt
    14221 tacctttgat tatgaaggat taaatgaagc cgctcagtcg tatacattca gtatcacgtt
    14281 tgaagttgct ttctttcaat caggcggtac tgagccaaat aatgactttc aagtgagtct
    14341 tacttatgct gagcgagcag caaacacgga attttcaatt acgcctattc cttcatcaag
    14401 acccattttt tcgaaattat actatggctc tagagaaatc gacagccaag gaaatcttgg
    14461 gaatctgctg attgacgggc aagacgtgac caacagtaat aaatttattt tgcaggtaaa
    14521 ttatgaagga tcacgaaaag acggtgtagt ggttaccgat gtactaccgc aaggaacaac
    14581 tttggcaccg aatccgacta catggagttc cactggaaat agagaaccta tttcaggtgg
    14641 ggttcggatc gcagaagtaa cgaatccagg tgaagcaaac gttagaactc attatgtttc
    14701 cgaggaattt gtggacaaag tatcttttaa taaggaaacg aatacacttg ttgtggactt
    14761 tggtaatcta gcagagggtc aatcgtattt tattgaatat gctcttgaag tgaccgatgt
    14821 cgacttgttc aatgaaggaa gacaagagga tgggttttct cggaatcgag cgagtatgac
    14881 ctacaataat caggtaatga gccgagacta ccgcttgaga atccctgaaa gtaatcttcc
    14941 ggcaatctct ttaagaaaaa gtgtggacaa aaatgttatc aatctcaatg aaaatgaact
    15001 aacgtatact ctgcatgtct cgatgttttc tggaacaaga aatacgcttg ttattgaaga
    15061 tgttttggaa gaaggcatgg aatttgtgtc atttctatca gatattccct ctttcattga
    15121 tgccgaagtc aataatggaa tagttcgttt cagtgcgaat aatacgattg gtggtccgac
    15181 aagttttacc ctctctttta aggtcaatgt agaggcgtat gcggtcggcg atcaaatttt
    15241 aaactttgca acgcttgtga atagtgacaa tcgattgaat acgagtactg taagaacaag
    15301 gaaaatcgat gggcgtatta aagtaattaa aactgatgag aatggccaaa ggctggcagg
    15361 agcaaaattt gaaattttat caagcgattc acaagtgata caagaaggta taacggcaga
    15421 aaacggagaa tttttaagtg aaccgcttcc tgtaggtagc tatcaagtcc gagaaacaga
    15481 agcaccggaa gggtatcttt tagatgcgac ggtacatgat gtttttataa atgaatactc
    15541 catagaagaa gtgacgttaa ccattgaaaa catccttgat acgggtggtg ttgaactaat
    15601 taaacatgct gaaggcagtg gtgaagtcct tcaaggagct gtttttgagt tacagaaccg
    15661 agaaggagaa acgctacaaa ccggtttaac tacaggagaa gatggaaaac tggcaattga
    15721 cggtttagca ccaggagcat atcagctggt agaaac
//
LOCUS       DACCYH010000065        15205 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.068, whole genome shotgun sequence.
ACCESSION   DACCYH010000065 DACCYH010000000
VERSION     DACCYH010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.068"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(787..1128)
                     /locus_tag="IVY85_001990"
     CDS             complement(787..1128)
                     /locus_tag="IVY85_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8045002.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1128..1358)
                     /locus_tag="IVY85_001991"
     CDS             complement(1128..1358)
                     /locus_tag="IVY85_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8045003.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1645..1983
                     /locus_tag="IVY85_001992"
     CDS             1645..1983
                     /locus_tag="IVY85_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045004.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2188..2427)
                     /locus_tag="IVY85_001993"
     CDS             complement(2188..2427)
                     /locus_tag="IVY85_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP8045005.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2403..2750)
                     /locus_tag="IVY85_001994"
     CDS             complement(2403..2750)
                     /locus_tag="IVY85_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP8045006.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(3482..4063)
                     /locus_tag="IVY85_001995"
     CDS             complement(3482..4063)
                     /locus_tag="IVY85_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8045007.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4246..4872)
                     /locus_tag="IVY85_001996"
     CDS             complement(4246..4872)
                     /locus_tag="IVY85_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8045008.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4894..6225)
                     /locus_tag="IVY85_001997"
     CDS             complement(4894..6225)
                     /locus_tag="IVY85_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP8045009.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6380..6781)
                     /locus_tag="IVY85_001998"
     CDS             complement(6380..6781)
                     /locus_tag="IVY85_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8045010.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6867..7073)
                     /locus_tag="IVY85_001999"
     CDS             complement(6867..7073)
                     /locus_tag="IVY85_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045011.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7168..8670)
                     /locus_tag="IVY85_002000"
     CDS             complement(7168..8670)
                     /locus_tag="IVY85_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP8045012.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8688..9143)
                     /gene="argR"
                     /locus_tag="IVY85_002001"
     CDS             complement(8688..9143)
                     /gene="argR"
                     /locus_tag="IVY85_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8045013.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9241..9858)
                     /locus_tag="IVY85_002002"
     CDS             complement(9241..9858)
                     /locus_tag="IVY85_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045014.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10079..11452
                     /locus_tag="IVY85_002003"
     CDS             10079..11452
                     /locus_tag="IVY85_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8045015.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11527..12639
                     /locus_tag="IVY85_002004"
     CDS             11527..12639
                     /locus_tag="IVY85_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP8045016.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12711..14348)
                     /locus_tag="IVY85_002005"
     CDS             complement(12711..14348)
                     /locus_tag="IVY85_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP8045017.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14516..15124)
                     /locus_tag="IVY85_002006"
     CDS             complement(14516..15124)
                     /locus_tag="IVY85_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8045018.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         4174 a         3130 c         2667 g         5234 t
ORIGIN      
        1 aagtgactca aaataaaaaa aaccttacat tatactggaa tgaactgtat tgatagtgta
       61 cctaaaagct ctactgacat aagatcgtgt aatcggtaaa gtaattttat taattatacg
      121 ggatgtaatc aattagagaa taaactaatc ataaaaggta cttttacatt cataattgtc
      181 gctttgagat tctactttgg ttcactatct gacccaattg cacattttcg atcattacaa
      241 gtattggtgt cgttagaaca actaaataaa aactagcggt agatttttca gaatctatgc
      301 taatttctgt atggatgttc cagtgatggc aaatttctct tttcattgtt tttcttgtcg
      361 catgatatgc ctttttgtgt tattcagtta aactatagaa attctttcct agacagatct
      421 tgatcccaaa ttttacgttt ttgagcatcg atatagttta aaatgacatt actcatatct
      481 aaagagttta gattttttag gactaaaact gtcagttgtt ctttttcttc tttctgttaa
      541 agtattgtcg cgacttttcg tatacgttaa gatatgtcgt gaaaaaacaa gttttgtgac
      601 acaaaaaaac gaatgacttg aggaaatcac ctcaatcatt cgtttaaaat gctatatatt
      661 ctttagttta agatacggaa attaatgcca tcgaccacac tagtagaatg aacgacattt
      721 acttggcctt tttttaattt cgttatgttt agcatataaa aatattatag agtttactta
      781 caatgcttaa aacatatact gtataatctg atcaatttta gccatgtctt gcaatggtaa
      841 gttttctatt tttttgagtt ttcgttcctt aaaatcaaga gattttaatt gtgtaataat
      901 tacttgtcct tttatatcta gctcttcagg taatgagtaa cgtgtcggta aattctttat
      961 agtggaagta attggacata tgaccgaaaa cattgtttta cggttaaatt cataacgaga
     1021 gacgactagt cctggtcgtc ttttttgaat ttcttttcct acggagggat caaaatcaat
     1081 ccatacaata tcaccttttt taggaatata aattctttct tctgacacta taaaatttct
     1141 ctcccttcag gagttaaatc ttcccattcg tctttctcat aatactcggc ctcttcccca
     1201 ccactaaatg gatcttcaat ttttggaact aacgtaattg ttccatcatc agaatacaca
     1261 acgatatatt cttggttttc tgaaggtttc tttccgttat cagaaggtaa agttaccact
     1321 acagaacttc cttgtaatct agattttgta acaagcatta tcatcactcc cttaataaaa
     1381 gtatactata taacactaag taacgcaaaa ttcccctttg taaactatct agaaaattgt
     1441 tttgtttaaa gtatatctta ttggatataa atagaaataa attgtagcca gttcattttt
     1501 tctttaacta aaaaaataat ccaagaaaca ttgaacctat ggatccaaaa gctacccaaa
     1561 tactatagct aaccccccgc taccttcaat tttgcagata aaacgctttg ggagaaaatt
     1621 tcaaagacaa caggccatcc tatcatgccc gattataaac aaaaagctgg ctattccttt
     1681 tgctctgaca aatttcaatt gggaaagttc acgaaactag caggtgaaga agtttcaact
     1741 gttcggatca aagaatgccc gcttcaaata gaaacattgg ttcaacgaat ttcaactaga
     1801 caagactttg ctattgtaga acgtgagatc gtagctattt ttgtagaaga agggatttta
     1861 catgatccaa ctcatatcaa tgtggataaa tggcaacctc ttatctataa attcagagaa
     1921 tatacaactg ccaaccagtc gttaactttg aattttagat tccaagaatt caaagagtca
     1981 taatgataaa catcgttagg tataaataaa aaagactcgc agcacgagta aaaaacaaat
     2041 agaagctata cattagaaag gtcgactgtg accctataga tgcagtatat gcagcttatg
     2101 agcatttctt ctgttctctt tgtttttggg atctgtgctg aagtcttttt cgttttttgc
     2161 cgctctttct ttgaaaaatc gattatgtta taccttctcc attactaatt caggcatctg
     2221 ctgagtaatg atttttcgcg caaaagaaat atctgttacc caaggttcga tttgttcttc
     2281 agtcaagaca agcggcattc ggtcatggat tggtgcgact gtgcggttag gttcagtagt
     2341 cattatgatt gattctgcca ttttttcatc ctgatggcta cgataaaaac cgccaatata
     2401 cactattttt tccttatttg aaaacataaa tttgtctttt tcttcattcc actcattggg
     2461 aaaacacaac gattttgcaa aaatggttga tgaaaggtct ttttttcttc tactgtctct
     2521 gcacgggcat taatcatcaa ttgtcctttt ttaaatccgg taaatcccca cttcatgatt
     2581 tccggagtga ggatgttttc ttcctctttt cttaacgtca caacaagatt tgacggaaaa
     2641 acttctccag ctgctaattc tgctgcctgc ttcggttgtt tcgtctgggc ttctcgataa
     2701 taattctgaa ggagctctga ctctagatca aaaaaataac gaccacacat cctaatccct
     2761 cccaatcttt ttttctatca tacactgcaa caagaagttc gcaccaacaa aacgctttca
     2821 atttttgtag aactaaaaaa gttgtgacaa gtgacaaact ctccagtgaa tggtattctc
     2881 ctgccaactc ctcggtcaca agtcacaata ttcaacaaac atgagaagct gctttttgaa
     2941 attgttcctt gtgactcgga gtgaaacggc aggcttacga cctctgggta aacggtgttc
     3001 aaaagccagt ttctcggtat taagccaaat ttatgcaaaa atgtagaaag ctattttcac
     3061 aaaatttttc ttaatactcg aaactaagcg actttttcac aacctcttcc ttaaacggtg
     3121 ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg aaaaatatga gcagctattt
     3181 ttatttggct gttcttatta cttgatgcag ggcagctttt tcacaacctc tccactaaac
     3241 ggtgttcaat cagctaacct tcggtattag ctcaaaaaac ggcaaaatat gaggaactat
     3301 tttgccgttt gtttctctaa tactcaggtc aaagcgctga tttcacaacc tctaatacac
     3361 ggtgttccag aagtagcttc ttcgaaaaat aagccgaaat ttcacaaaaa tttgagaagc
     3421 aattttcgta aattccttct tatttcttga agctgaccac ttctgtcaca acctctaatt
     3481 attaaagaac cttttgcgaa gttaacaatc tttctttgat caattgaaaa ttctgcctaa
     3541 gtagtgatag atcctgtgtg actttcaaca ataagatact tccctggatt tcagcaatca
     3601 acgctttggc ttgacggtct gcttcttcat cggacagttt ttcttcagaa agtgccagga
     3661 tattttgttt cgtatacttg acccattgat tgactaattc tagtagcaac agacggtatt
     3721 cttcatccac tgtactcaac tcaataatca aattacctac aggacagcca tgataagtga
     3781 tctgtgtctc atgatcagcg aaaatccagt ccaacatttc ttctaatgct tcttctggct
     3841 gttttccagc atttaagatg tcttcgaaca attctgtctg ccatacgtcc atatgcgcat
     3901 ggatcaccgc tagacaaact tctttttttg aagaaaagta ataataaaat tgaccttttc
     3961 ctgtatcaga agctgctaga atatctcgga ttgacgtttc atgatagcct tttttataaa
     4021 taagatcttt ggtcgcttcc aagatctttt cctttgtttt caatgatatt ctcctttttc
     4081 atttctcata gtcctatatt atacagtaaa ttcatcattt tttcttctta atcgtttaaa
     4141 aaagactgtg ccaaattatc tgacacagcc ctcactataa aactgttctc atatagctag
     4201 atgttcattg attcaagaac tgcgaggaca aaagcaattc ctatcttatg atagttgctc
     4261 taatacttcc tctgttgtca tcacttgatc tgcaataaag ccaaagttcg tcaacgctgc
     4321 atccattgct tcttggccag gagcaccaat tgcatctgtc gctaccacaa cattgaatcc
     4381 actctctacc aattcacgca tatgggaatc gctacacaag tttccgttca ttccaccaag
     4441 aattactgtg tcgattccat tttttctcaa ttggagcgcc aaatcattgc tttctggtcc
     4501 aaagatttta tgaggagagc tgatgatcgt attttcatcc aaataaggtt tcaattcttc
     4561 gataaaatcg gctcctgaac cttcttccac tgattcatat tgactttttc tagcaaacat
     4621 ctgctgctca agcatcatat gttctcctgc accgccaaat tgccatttat ggtcatgtgg
     4681 gtaaaaataa tgaggagaaa taaagagttt gtatcctttt tcttttgctt gttttaccat
     4741 ttcaatcata ttttcgatcg tattgtattt ttctaagata tcttttgtca aaccaaagcc
     4801 accgcctgct ggtttcaaga actcgacttg cgggtcagtg agaactactg ctgtattttt
     4861 attattgaac atggtggatt ctccattcgt tatttatttt aatacatcat ttagagcttc
     4921 actcattgtc ggatgagaat agatttgatc tctcaataac gtataaggaa gttttccatt
     4981 catagctagc gaaatcaagt tgatgacttc atgagaatct tccgcataga tacttgcccc
     5041 tagaatcaag tcagtttcag ggtcgactaa aactttgaat acacctttag gatcttctaa
     5101 tacttgtgct ttagggacac cgcttgtcat atacttgaat aatttgtaat tgacgccttg
     5161 tgctttggct tgtttttctg ttaaacctac attggataaa gtcggagtga taaatacact
     5221 gtaaggcaca gctttccggt tattcgttgt tcgtttattc tcgcccttca actgatccat
     5281 aatgattcga taatcatcta gagagatata agtgaactgc aatccgcctt tgacgtctcc
     5341 aatcgcccaa atgttgtctg catctgtacg aaggtattca tccacagcga ttgctccgcg
     5401 atctgttagt ttaacatctg tattttctaa tccaagtgat gctgtacttg gttttcttcc
     5461 agttgcagcc aaaacttttg acgcttggat tgttacttct tggccatctt ctgtaaaggt
     5521 gacggcagct gttgattctt ggtctgctac ttgatctaca gatacaccta gatggaattc
     5581 gattcccgca tttgtcatgt cttctaaaat catttgcgcg atatcttcat cttcacgtgg
     5641 aagaaattca gaatgggcat caagaacgac aactttagat ccgtattcat tgaacattga
     5701 tgcaaattcc aagccaatat atccagcccc gatgatcact aatttttccg gtaattgttc
     5761 ctgatccatc gcttgcgtag aatcaatcaa gtagcgactt gtattcaatc ctttgattgg
     5821 taaaatgatt ggttcagaac cagtattgat gaaaatccgt tcgccttcaa caatcacttt
     5881 ttcaccatta ttcgtcactt cgatttgatg gttagaaaga aaacgtgctg taccgtccat
     5941 cactgtccca gttgcctcat cgctgatcat atgataattt ttggcgcgaa gcatgcccgt
     6001 caatttttct ttccgagcaa cagcctctgt aaagtcgatt cctctttctc cattgaaaat
     6061 caatgattta gaaggaatac agccgatgtt gatacatgtt ccgccataca tacgtgttga
     6121 cttttcaacg actaaaaccg attcaccttt tgaagctaac gccttcgcta aagtcttacc
     6181 gccctttcca aaaccgatca caatattttg atatttttcc atcatttttc cctcgcttct
     6241 tttttaccta ccgattggta caaaaatatg ataaccgata attgtctatt ctccaaatca
     6301 tctgctttat ttgtcaaaaa aaaccagaca aaaagcggtc ctttcacgtt tttagtctgg
     6361 ttcgatttcc tttattcttc taaacaatcg ttacttctac atcgatattt cctctagttg
     6421 ctttggagta agggcagaat tcatgcgcat cttctgcaat tttttgggct tcttcttcgc
     6481 tgatgccatc gatatgcact tctaatacta caccgatctg atagccgcca tcatctaaat
     6541 tataaagaga gacacgtgct tttactgtgg actctgctgt cattcctgct tctttgatca
     6601 tatgttgtac cgccccattg aaacaagaag cataacctgc tgcaaataat tgttctggat
     6661 ttgttccttc tgcatggatt cctggaggag tgattttcat atgcatcgag ccatttggtg
     6721 cctcgacttc tccattgcgc ccgccatggt tgatcattgc tgtttcgtat gcttttttca
     6781 ttgtactgct cctttcgatt gaccatttgc tacttggcta taattatagt gtatgacaag
     6841 taaggcgctt tctacaaaaa ttctgcttat tcgaatacca gaatgccgtc ttctactatt
     6901 tttccaaaaa acaaaaaaat actactcgtg aacaacagcg tcatatttgt tccaaaataa
     6961 atagcaatct tttctacata atcttttata tgattgattt taagatacat atataccaga
     7021 aagttgaccc cataaaccaa tactgttaaa aaaataacca tattcgttac cattttttcc
     7081 tccttttgct ggtctcccta ccagcactcg atttaaccat accacttttt ttgccgtcca
     7141 agcaactagc ctttcaaaaa gactggttta ttctgctgtt aatgattcaa gatcagaaaa
     7201 atgtccgaat aattttgcta attttactaa cgccaacttg ttctttccga attcatcatt
     7261 ttctatctgt aatactagta acggctcatg taagcttaaa cggatcaaaa accagccgtt
     7321 tccgtatggc tcttctagat catatcgaat accttcttca ttttccgggt cgaagttcat
     7381 ccctttttct tcttccaaca gtcgcttcat ttcttctaat atcttgtttc cttgttcgcg
     7441 tggttcatcg ctgaacaatt ttaatcggat ttcttgtact tctacgggct gtttcaactc
     7501 tttgatttgt tcatctaatg tgataccttt tctttttaat tctggcaaaa gcatcaggat
     7561 ctttgcgatg acatatgcac catcatccag aaaatagttt tccttgaatg cagcgtgtcc
     7621 gcttgtttcg atggctagct gtgtatcttt cccaagacgg ttaagttcta atgctttatt
     7681 gattacattt cgatagccgg aaatataacg aacttggatg cctccttttt cttcaatgaa
     7741 tctttgtaga tgagaagaag taggggaatt cgtcacgatt cttgttcctg gatgctcttt
     7801 taatacgatt gtacttaata ctgcgatcaa attatttcga ttgatcactg ttccagaagc
     7861 actgacaact gcagaacgat ccacatctgt atcgaaaatg atcccaagat ctgcttcatt
     7921 tgctaaaaca gcctgcttga tactttccat cgcttcttta ttatcaggat ttgggatatg
     7981 attaggaaat cggccatctg gttccaaaaa ttgggaacct gttgaatccg cacctaagct
     8041 attcaatact ttttcagcaa agaatccacc agcaccattt ccggcatcaa caatgatttt
     8101 ccaaccattt agtggtttgc tttctagtgt tccaatcccc tttttaattt ttgtgactaa
     8161 atcttgcgca taaggtgtca aaaggtctgc tttttctacg cttccagttt gttcgctttt
     8221 tattttttgc tgctgtgata ggataaaagc aatatcttct ttttctgctc ctccttctga
     8281 ggtaaagaat tttattccat tataataata gggaagatgg ctagcagtca gcataaccgc
     8341 agcatcgcac gagaactgtt caaattgagt ggacataaac atagcaggcg tagtagctaa
     8401 ttcgaaatcg atgacatgta tcccttgttc tttcagactt tggatcaacg cctctttcag
     8461 tgctggtccg cttatcctac tgtcatgacc aatcccaatc gtccattttt tatttggatc
     8521 ggttgatttc ttctttttct ttatccagtt tgctagtccc cagccgatct tttttaccat
     8581 gtctgtcgtc aagtttactt catatccttc aactggtaat gcgattcctc gaatatccga
     8641 tccattttgc agcgctttca tatctttcat ataaaagtcc tcctcattta ttccctcatt
     8701 ttttccagct tttctgcaaa tcgctgtgct tcttcttctg agcggcaaat aataatgaat
     8761 gtatctacac cggccatcgt tccgacaact tcagggtaag ccacttcatc tagccaattc
     8821 gttaccacat cggcaccatt tcgttcagtc aatacgataa ccatgaactg aacagactgg
     8881 atacgcaaaa cctcgcgtct aattgctgta cgcaaacgtt cttcatttaa agaaacagat
     8941 ggctggttaa ataatgtata gcgtactatt ttgttttcat tttgactttt tactaacttc
     9001 atttctcgaa tgtctcgaga aatcgttgct tgagtcacat cggcgcctgc ttcatgcagc
     9061 cgcgcaagca gctcttcctg tgtcccaata ggttgttctg taatgatctg gcggatcagt
     9121 gcttgtcgct gtttcttttt catattagac acctctcaat gttgattata gcaaaagata
     9181 agtaatattt cttcatattt tggtattttt tacttttatt ttttgttttc attcaggttg
     9241 ctaacctttt tctattttaa cacgcaaaat cgtcttcatt ttttcaggtg ctgattttct
     9301 tggatcgctg atgtaaatct ctttgtgttc tttagaagct ctttcatagc cttcttttgc
     9361 gatgaatgct tctaatttct caaacgttgc tggttcttcg gcatagggcc cgacgtgtaa
     9421 gatttgggca caaagacctt cttccatctt tttgaatact acttgttctt tcaagtcttc
     9481 cggaagtttg ctagaacgct gaatggcttc ttgtgctatc gcttctgtaa caaagtcagg
     9541 ctgtcggatc atcaattgat aagaaaaatg ttcttttttc atcactggtt catgcaaata
     9601 cttttcttgc agtccccatt gtccttccag tggatagaca gtatacatct gataatcagg
     9661 aatcagccaa ttttttttag gcgacatgcg tattgcatag ctgactgcat atagacaggc
     9721 tactcttcgt ttaaagtctt cctgattcgg gtcgccgatc cctctgatgc taaagaatga
     9781 ttttttagga acctctagta tttgcgggct ttttttcata ctatagctct tgtctttacg
     9841 ccattcaatt ttttccattt ttcttcctcc gcgaaattcc atttagttat ttattatcat
     9901 acctcttttt gagtgacttg gctaatcgtg ttgacactat ttttcagttt gtgctagaat
     9961 aagtgaagta aataaatcgt acagataaca cgtttcggcg tgtcgagaga gaggcggttt
    10021 ctatttttat aatagaaatc actctctttt tttacgaaat tttttagagg tgatgtaaat
    10081 gacaaaacaa aaacagctgc ttttagtacc tgcagtaatt gcaacaggga tgatgtcttt
    10141 tgcgggcgta ttgatcgaga cggccatgaa tgtaactttc ccaactctta tcagacagtt
    10201 tgatttaacg actgcacaag tccaatgggt aacaacgatt tatcttttga tgatttcgat
    10261 cattgttccg ctgtctactt atttgaatcg caattttagt ctcaaaaaac tctttttagc
    10321 ttcaaatctg cttttcttgg caggtataat gatcgatttc ttctctccta ccttcatggt
    10381 cctattgttt ggtcgattgc tccaaggtgc agcgacaggt atcgctcttc cgttgatgtt
    10441 ccatattatc ttgacatttg cccccctcaa tcgacgaggc gcgatgatgg gtgtcggtac
    10501 tttgacaacg gccattgctc cagcaattgg ccccacttat ggaggcatta tgacttcaca
    10561 ttttacatgg aatcatatct tcctgtttct tgtgcccgtt ttgtttcttt ctttgattat
    10621 cggctggttt gctattcccg agatggcagt acaaaaaagt ggttatttgg attggataag
    10681 cgttttagga atttgtcttt tgtttagtgg aagtctaaca tttttgagta cgttgggaac
    10741 agtttccagc tggattgctt taatcatcgg cggtattggc tggttccttt tttactatcg
    10801 aagtaaaaaa gtggagcatc cactgatccg actgtctatt ctttcaaata aaacattccg
    10861 cttgtttttg attggcttct tagtttatca gtttttatta cttggtgtat cttttgtctt
    10921 gccgaatttc cttcagatcg ttcaaggaag tagtcctttt gtcgctggat tagcgatgct
    10981 tcccggcgca gcggttggtg ctttgctttc tcctttttcc gggaaaatgc tggatcaata
    11041 tggtccgaag aagccaattt ttgctgggct gattctttct ttgattggtt ggctagcatt
    11101 attcctactc ataggacacg cttcagtggg tgtattgatt gcttgtcatg tgttttatat
    11161 gatcggtatc ggattatctt acagcaatat gatgaccacc ggaatgaatg cactgaatga
    11221 agaactacaa ggagacggga acgcaatgtt caataccttg cagcagtttt ctggcgctgt
    11281 ggctacttca ttagtagcgg tcattatcaa ttacgtccag catcatactt cccataacta
    11341 tgaagtgtct actacattag gatcaaaagc agcattaggc gtcttgttgc tgttgcttct
    11401 cgtaagcttt gctcgattcg cctattatct gttctttgct aaaaaagcgt agattccata
    11461 tacggcaggt gttgcatctg ccgttttttt gtgtgaaaat ggttgtttgt aaaaggaggc
    11521 ttttttatgg aaaaaattgc cattatcggt ggcgggatca ttggtatgac cctagccagt
    11581 tacttagata catcaaaatt caatgttgct ttatttgatg aagggacagg acaggcaact
    11641 aaagctagcg ctgggattat ctccccttgg ttgtcgaaac gcagaaacaa acaatggtat
    11701 cgtctggcta aagacggcgc tgcttttttc cctaaactta taaaagattt ccaacttgat
    11761 caaacgattt acgcacaatc agggacactt ttgcttcgac ctgaaaaaga tttagctgaa
    11821 ttggctgttc ttgccgagaa acgaaaagaa gaagcgccag aaattggaaa catcgaatgg
    11881 ctttcacctg aacagacatc tcaagcactt ccgttattga aaccagcagt ttcactgcgt
    11941 atcagtggtg gtggtcgtct ggatggaaaa gcatatttag aacatcttgg tcagcgttta
    12001 gcacaaaaac aaatggagca gatccaggca aaagtcactt tgcataagaa gagcgattct
    12061 tggctcatca agggaccgtt tggagaatac acagctgatc gtgtaatttt gacacctggc
    12121 ccggcactta agcctttatt acaagaatta ggctatgctg cagatatacg tccgcaaaaa
    12181 gggcagctct tagcatttca gacatcttat aagatgagtc aagactggcc tgttgctatg
    12241 cttgacggag aagctgattt cattcctttc atggatggaa agatcctttt aggtgctacc
    12301 catgaaaacg atcaaggctg ggatttggct cctactagcg aagcttataa gggattgact
    12361 aagagtacag aacagttctt gactgatcca gatcagataa aaaactggcc atacgagttc
    12421 cgtgtaggta cccgtgctta tacgtcagat ttttccccat ttttcggcgt actgccagat
    12481 gatccaagct tactggctgc tagcggtcta ggctcttctg gcttgaccac aggtccttat
    12541 atcggctatc tattagccaa cttcttaaat actggtcatt ggcctacgga agattatcaa
    12601 aaggacatct ccacctatat tacgaaaaat ccatcataga aaaacagctt tttcaagtaa
    12661 ggatcccgag cgatattgcg catggattac ttcttgaaaa agctgttatt ttagttcaat
    12721 ttctgaatca aattctccaa actttctttt gcttcatctg gcaagagatc ttttcctatc
    12781 tgagcttcaa tgaatcgtaa acgaatatca agtcgttgtt tcaccagatt tttataggac
    12841 gtatcctcaa aagaaacagg aaaatctggt ataggtaaat agtggatgtc ttcaaatgga
    12901 ccaaacgact tgaattctgc cgtttgttgc acgattcttt caacgattga caatgtcgtt
    12961 tcaggtttga tttttttatc atcgaaatat ccagtattca ggacatagca ggctgtttgc
    13021 tgtttcaaaa acaactctcg aaaagcttga tagtcttccc taagaggata gacacggaaa
    13081 ggattagcaa atggttcaat gactagctca tctagctgtc tttttgtagt gatattttcc
    13141 gcgtctgatc gttttgttgc taatgttgca ccaaaagacg ctgctaaaac tgggtcattg
    13201 atcttgataa ttggcggaag cgaatcatct ttcatgatcc aaaaaacagc atcgatcttt
    13261 tcgctaagat gatcgactcg gtcgggtgtc gcataacgtg atttgactgt ccgcccattt
    13321 ccgtttcgta tatctcctgt tactaggact ttttctcctt tacttgtaca tgtcacgccc
    13381 acattctgac aagtaaggaa agaagaaatg gccggatcat cagacggata gtcttgtgtt
    13441 ttatcaaagt aagaaggttc caaggcagta gcaaaaccat tttttcgaga aataacgaaa
    13501 gcatcgtcat gcaagatcga aacagagtga ccttccgtat gttttgctaa ggtaatcgtt
    13561 gattttccag aaccggacaa cccaaataca gccatcgtat aggtctggtc attactagca
    13621 tattgtttca tcccaccgtg gcaggcaata aagccattgc gatttcccaa tgcccaagca
    13681 agcgtcaaag tcgctttttt taattctcca aagtaacgta atcctagcac agccgctgta
    13741 ttatggagag catcaaacaa aatgagtcca tttggaaatg tttcatgtgt ccattcggga
    13801 tcacaaaaga tataaagatc tccctcatca aaggaaacag acttttgata agtctctaag
    13861 atcttcgaat tcgctggctg aaaattcagc aaataagaat acaaattgtt ctcaaatcct
    13921 tctggaatca gcaaatgact ttgcacagaa aattcttcag ataggccaac gattactttt
    13981 gtatggtaaa actggcgctt gcgactttga tacacagctt ctcttaaaat tttctcgtat
    14041 ttgctttgtt ccttgtctaa gtcctcaaag atccgccgag cacttgctgt tcttcccaca
    14101 atgcttccat cattgaatac tagtgtccta acatcagctg gtaaatctag ttcttcagca
    14161 tgtgagattg ttaaatctgt gatgatcgtt cctggactcc cttttgctag ttgataagct
    14221 gtcttcctat caaaaacttc atgaacatta ttaccgtaaa atgtcgtttc tacaatcgat
    14281 cgaatactgg ataacagtgg atttccactt gtgatttcgt ttaattcgag actctctttt
    14341 gtactcatgg ctcctcctca ttccctaaac aaaaaaacgt tttctttatt atacattatt
    14401 cgagttttta tttgtactgc tgcgctgtgg gaagcaaacg agttattttt cacacaaaaa
    14461 aaccgagaac ttgaataaac aagtttctcg gtttcatcat tttcctttta tcggcttagt
    14521 accagccgtt tgctaaccag aatgttttag cagcatccca tgaaccataa cgagatgtta
    14581 catattgttc tgctacacgt tcttgattgg cagctgaata gtcgccgttt aagtatgaag
    14641 catctaattg ataacggccg atgtaacgtc cgttagtagc tgtgtatgaa ccgcctgatt
    14701 ctttttgtgc gatccattct tttgcagagc ttgaagttgg cgctgttgca gcagctgggg
    14761 cagcaggagc tacttcaact gtttcaacag gtgcaacgta tgtatgttct tgcgttgctg
    14821 tttcagttgt agatgctgta ttttctgttg ttttgttttc tgttgttgct gaagaatcat
    14881 ttgaagttgg gattgttaat tgttcaccaa cataaatgcg gttgatgttt gcgatcgaat
    14941 ttttttcagc aattgcatcg atcattgagt tgtctcctgc aaatttttgt gagatcttag
    15001 ataatgaatc acctgatttt actgtataaa cttcatcagc gtgagcattt gtagttccta
    15061 caaacattgc agtagcacca gcagcaaaag ttgtacctaa aacaattttc ttaaatgaat
    15121 tcatattaaa ctcctttttt tctccgtttt ttattctttc gacgctatta actttatcat
    15181 ttagaaatat ttcataggta aaaga
//
LOCUS       DACCYH010000066        14933 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.069, whole genome shotgun sequence.
ACCESSION   DACCYH010000066 DACCYH010000000
VERSION     DACCYH010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.069"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(90..1649)
                     /locus_tag="IVY85_002007"
     CDS             complement(90..1649)
                     /locus_tag="IVY85_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP8045019.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            1804..4038
                     /gene="pcrA"
                     /locus_tag="IVY85_002008"
     CDS             1804..4038
                     /gene="pcrA"
                     /locus_tag="IVY85_002008"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP8045020.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            4055..6091
                     /gene="ligA"
                     /locus_tag="IVY85_002009"
     CDS             4055..6091
                     /gene="ligA"
                     /locus_tag="IVY85_002009"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP8045021.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            6197..6502
                     /gene="gatC"
                     /locus_tag="IVY85_002010"
     CDS             6197..6502
                     /gene="gatC"
                     /locus_tag="IVY85_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP8045022.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            6502..7974
                     /gene="gatA"
                     /locus_tag="IVY85_002011"
     CDS             6502..7974
                     /gene="gatA"
                     /locus_tag="IVY85_002011"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP8045023.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            7971..9401
                     /gene="gatB"
                     /locus_tag="IVY85_002012"
     CDS             7971..9401
                     /gene="gatB"
                     /locus_tag="IVY85_002012"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP8045024.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            9411..10466
                     /locus_tag="IVY85_002013"
     CDS             9411..10466
                     /locus_tag="IVY85_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8045025.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            10577..11950
                     /gene="rlmD"
                     /locus_tag="IVY85_002014"
     CDS             10577..11950
                     /gene="rlmD"
                     /locus_tag="IVY85_002014"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8045026.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            11953..12081
                     /locus_tag="IVY85_002015"
     CDS             11953..12081
                     /locus_tag="IVY85_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8045027.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            12521..12709
                     /locus_tag="IVY85_002016"
     CDS             12521..12709
                     /locus_tag="IVY85_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP8045028.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            12787..13800
                     /locus_tag="IVY85_002017"
     CDS             12787..13800
                     /locus_tag="IVY85_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8045029.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(13867..14901)
                     /locus_tag="IVY85_002018"
     CDS             complement(13867..14901)
                     /locus_tag="IVY85_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8045030.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
BASE COUNT         5062 a         2776 c         3176 g         3919 t
ORIGIN      
        1 gttttcccag cctcttcttt ttttatagaa tcatatagaa tcaagtagtg tagatgaata
       61 gttgttgtaa aatagttttt ccatgttcgt taaaatgatt gcttcaaatt tgtgatagaa
      121 gatttagcct cgatttcctg cttgattctt ggcaagtttg cttcatcagt caagataacc
      181 aaaatatctc ctacattcat gatcgtatca ccgtgagtca agatttcctg atttcctctg
      241 cgtatagaga ttagcagcat atttttaggc caattgaagt ctcgtaccat taatccgtct
      301 aaagaactct ctgccgttac tggaaaatca aaagttgtcc ttgcaccttt tagtgaggcg
      361 gtctgagtat ttccaagtag acgttccaat aatgattcgt aaattggatt accgccgaga
      421 aggtcattga cgatataagc aacaagagca gccaatccta aaggcatcag atgattaaga
      481 gagccaacca tttctgtcac taaaataatt gctgtcagcg gtgcttttcc aatcgctgtg
      541 aaataaccag ccatggcaat aacgacaaaa tccttgatgt agattggatc catcccgaaa
      601 tcaaccataa tgtttccata aaagctaccg attaaagccc ccaatgtcaa tatcggaagg
      661 aaaatccccc ctggaagacc cccaccgtaa gaaatcatgg aaaaaacgaa tcgaagaaca
      721 aacaaaccga taatcataat gattggcaag gaagcatgtc caaaagctaa aacgatttga
      781 ttgcctccgc ctagagtact aggccataaa taaccaattg gcaaaatcag caagaaagga
      841 acgaacccgt gaaaataagc tggtaaaggc aatcggttgt aaaattttgg caaagccagc
      901 aagacattct gataaataaa acctaagatg cctagaagta ctcctaagat cactaaggaa
      961 ccataatatt tcaacggcaa agaaggaagg tcacctagat ataaaacagg tctcaagccg
     1021 aaaaagtaaa gagagacgaa gttagcagtg attgctgaag caaatgatgt cagccaaaca
     1081 agtggagaaa aggagtgatg gatttcttct aaaacaaaga gtaatcctgc gataggagca
     1141 ttgaatgctg ctgcaagtcc gctacttgca ccgctagaaa ttaaaatctt ttcttctgaa
     1201 gatggactcc gaaacaattg tccagctcct tgtccaatgg atgcgccaag ctgtatagaa
     1261 ggtccttctc tacctagaaa aaggcccgca ccaactgaaa gtacaccgcc aatgaacttt
     1321 ttccataaaa cagagaacca gttcattgat aattccccgc gaagctgtcc ctctacttgt
     1381 ggaatcccgc ttcccttgat atccggttca tttttgacca gataacccac gaaaaatgct
     1441 gcaaggatgc agcaaatagc taggccaatt aagtaaatcg gctgttcccg actcatttca
     1501 tagatacttc gaactaaatc gcttagcttt tcgatagata atcgaaacag gctaacgacg
     1561 attcctgtca gcagtccaac aaatattcct tttagaataa aatatatctg agtcttatct
     1621 aatttcttga tttttccttt tttttccatt caaacgcctc gctttctttt aatttagcgc
     1681 gaaatcaacg aattgacaag gaaaaatcca taaaaagaaa caaatgattt tgaactagct
     1741 agtttaatga aagccaagaa aatgctataa ttaacaagat tgataaaaga aaggatgttg
     1801 ttaatgtcat ccaaagctga attattaaac gggatgaacc cccgtcagaa agaagctgtt
     1861 cttcacactg acggaccatt actgctaatg gcaggtgcag gaagtggaaa gacgagagtg
     1921 ctgacacacc gcattgccta tttgattgag gaaaaagaag tcaatccttg gaacatccta
     1981 gcgattactt ttaccaataa ggctgcaaaa gaaatgaaag aacgtgtcaa tgcgattctc
     2041 gcatctggag gcgaagatgt ctgggtatcg acttttcact cgatgtgtgt gcgtatttta
     2101 agaagagatg ttgattttat cgggtataac cggaacttta caatcatcga ttcttctgaa
     2161 cagctgacct tgatgaaacg gatactaaaa gaactgaaca ttgatccgaa aaaatatgat
     2221 cctcgaagta tattaggaac aatctctcaa gcaaaaaaca gcctgcagac ccctcaggat
     2281 tttgcaaaaa tgcaaggcag ttattacgaa gagattgcgg caaaatgtta tgctgcttac
     2341 caaaaagagc ttcaatataa tcaatgtatg gactttgacg acctgatcat gaatacgatt
     2401 cgattatttg aagaacatcc tgattcactc acgtattatc aaaacaaatt ccattatatt
     2461 catgtagatg agtaccaaga tacaaatcac gcacagtata cgctagtcaa tttattggca
     2521 ggtagattta ggaacctgtg cgtcgtggga gatgcagatc aaagtattta cggctggcgc
     2581 ggtgcagata tgcagaacat cttggatttt gaaaaagatt atccagatgc tgcagtaatt
     2641 ttgctagaac aaaactatcg ctccacgaaa aacatcctta gcgcagcaaa ccaagtcatt
     2701 gaaaacaata gcaatcgaaa accaaaaaac ttgtggacag aaaacaaaga aggaaacaag
     2761 atcacttact accgtgcaga taatgaacga gacgaaaccc gttttatcgt agaccggatg
     2821 caagaagaga ttcgaagcaa tcatcggaat tacggcgact ttgccatctt gtaccggacg
     2881 aacgcacaat ctcgtgtaat ggaagaaact ctgttgaaag caaatattcc ttataaaatg
     2941 gttggagggc acaagttcta cgatcgtaaa gaaatcaaag atattttagc gtatttgaat
     3001 gtgctggtaa atcctcaaga ttcaatcagt tttgagcgta tcgttaattc gcccaaacga
     3061 ggcatcggac caggatctat cgaaaaacta cgcagttttg cttctcttca tgaatggcca
     3121 ctgcttgaag cagcgcaaaa tgtggatctg gcaaatatcg gtggaaaagc aggacagcaa
     3181 ttaggtgcat ttggagagat gatccaagaa gtcactcaga tgatcccata cttgacagtg
     3241 acagaattga cgaaagaggt tttggatcgt agcggatatc tagaagactt aaaaatccaa
     3301 aatacattag aagcacaagc acgcatcgaa aacttggagg aatttttaac ggtcacacaa
     3361 gaattcgaca aacaattcga acagcaaaat gaagaagacg ctgatgcacc agaagaaaaa
     3421 ctaacggtct tcttaaatga tctggcactt gtttccgaca ttgacaattt agaagaagat
     3481 gcttcccaag tgacattgat gactttgcat gcagccaaag gattagaatt tccagttgta
     3541 ttcctgattg gtctagaaga gggggttttt cctttatctc gagcattgat ggaagaaagc
     3601 gaactggagg aagaacgccg tctcgcatat gtggggatca cgcgagcaga agaagcgttg
     3661 tatttaacaa atgctttctc cagaacatta tatggacgga cgcaatataa ccgaccttct
     3721 cgttttgtag aagaaatcga tcaggagtta ctggaaatcg aaggcatgcg tcctacgcct
     3781 aaaaaaacgc ctgtattcgc taaaaaaaca gcatatagct ataaacagcc agagacagcg
     3841 gttgttccct ctaaaagtgc tactggaggg gaaaataaca gctggaaacc tggagataaa
     3901 gtcaaacaca aaaaatgggg tctaggaaca gtcgtccgag taagcgggac gtcgaaggat
     3961 ctagaactag atgtggcttt cccaagtcaa ggagtaaaac gtcttttggc tgcctttgcg
     4021 ccaattgaaa aagcttagta gtggagggta aatgatggaa catgaaatga cgattgacga
     4081 agctgctaaa cgagcagaag aacttcgtac aagactgaac caatggtctc gcgaatacta
     4141 cgtagaagat aaaccaacag tggaagacta tgtttatgat aaagaatacg cagaattagt
     4201 agcaatcgag gaacaatatc ctgatttgat cacgtctgat tctcccactc aacgagtagg
     4261 cggaaaagta ttagaaggtt ttgaaaaagt cactcatgat attccgcttt acagtttgaa
     4321 cgacgttttc agcaaagaag agctgatagc ttttgatcaa cgtgtacaaa aagcagtggg
     4381 acgtgtagtt gactattgct gtgaactaaa aatagatgga ctttccgttt ctttgcgtta
     4441 tgaagatgga aatttcgtga gaggtgcgac tcgcggggat ggaactgtag gagaaaacat
     4501 cactgaaaac ctaaaaactg tccggtcggt cccaatcaaa ttaaaagaac caatgaacat
     4561 cgaagtaaga ggcgaatgtt tcatgccaaa acggtcattt gtccaattga accaagacag
     4621 agaagcagag ggaaaagata tctttgcgaa tcctcggaac gccgcagctg gttctttgcg
     4681 tcagttagat tctaaaatca cagcgaaaag aaatctggac acttttttat acacagtagc
     4741 tgatttcggt cctatgcaag caaaaaccca gtatgatgcg ctagaagagc tagaaaaaat
     4801 tggattccac acgaatcggg aaaaacgtct atgtcattcg attgatgaag tgtggtcata
     4861 cattgaagaa tatcatgaca aacgtgtgga tttgccctat gaaatcgatg gaatcgtcat
     4921 caaagtgaat gaattctcgc tacaagatca acttggtttt actatcaaag caccgagatg
     4981 ggcagcagct tacaagtttc cgccagaaga agttgaaacg ctaatcgaaa acatcgaatg
     5041 gacagtcggt cgaacagggg tagtaacgcc aacagctatc atgacaccag tccgagtagc
     5101 aggaacgaca gtaagccgtg ccagcctgca taacggtgat tatattaagt tgaaggatat
     5161 ccgcttgaaa gatacagttc tgatttataa agcgggagac atcatcccag aagtttcgca
     5221 agtcgtgtta gataaacgac ctaaagacag cgaagaatac caattgccta cacattgtcc
     5281 agtttgtgga agtgaacttg tccatttgga tgaagaagta gccttacggt gcatcaatcc
     5341 taaatgccct gctcaaatga aagaaggttt gaaccacttt gtttcaagaa atgcgatgaa
     5401 tatcgatggg ttggggccgc gcgtactaga acagatgtat gataaaaaac tagtagcaga
     5461 tgttgctgat ctttacaaat tgacagaaga agagttattg acgttggaca aaataaaaga
     5521 aaaatcagca aataatattt tgacagcaat cgacaacagc aaagataatt cggtcgagcg
     5581 gttgattttc gggttaggaa tccgacatgt cggagcgaaa gcagcaaaaa ttttagcaga
     5641 acatttcggt gatctagaaa cattgagtaa aagtgactat gagtcgatta ttgcgcttga
     5701 tacgatcggt gatatcattg ccgacagtgt agtcacttat ttcagtaacg aagaagtcca
     5761 tgaattgatg aatgaattaa aacaagcagg cgtcaatttc gaatacaaag ggcttcgaaa
     5821 tgcccaattg caagaagtcg agtctccttt taaagaaaaa acagtagtcc ttactggtaa
     5881 actaacacga tttactcgtg aagaagcaaa agagacgatc gaaaatcttg gcgggaaagt
     5941 gactggtagc gtatcgaaaa aaacggatat cgtggtagca ggagaagatg ctggcagcaa
     6001 gctgaccaaa gcacaagaat taggaatcga agtttggaca gaagatcaaa tggcagatgc
     6061 attggctaaa agtcgctcag tagaagaatg aaaaatgagt tttttcagac aaatttcaat
     6121 aaacaatttc agacacggca ctcttgtgtt aaaatagaga gcaaagtttt taactcgttt
     6181 agaaagaagg ggtagtatgg cgattagtga agaacaagtg aaacacgttg ccaaattagc
     6241 aaaacttgcg ttcgcagatg aagaacttgc aagttttacc gaccaattag gtaaaatcat
     6301 cgatatggtt gaaatgctgg aagaagtaga tacggaaggt gttccattta cgtcgaatgt
     6361 ggttgaaaca gtaaacgtga tgcgacaaga tcgggcgcaa gctggatgga accgcgatga
     6421 attattgaaa aatgtgccgg aaacagaaga cggctttatc aaagtacccg caatcatcga
     6481 taatggggag gctggcgcat aatgacagaa ttacacaatc gttctctaga agaactgcat
     6541 catatgcttg tatcgaaaga aatcactgta caagatttga caaaaacaac atttgaacga
     6601 atcaaagaaa cagagcctga ggtcgaggca ttcattacat tgaacgaaga aaaagcattg
     6661 gaacaggcaa aagctttaga tgaaaaaggg attgatgaaa gcaacgtcct tgctggtatt
     6721 ccaattggta tcaaggataa tatcgtaaca aaagatatct tgacaaccgc tgcatctaag
     6781 atcctttaca attttgaacc aatttatgat gcaacggtca tggataaaat ctatcaagca
     6841 gatatgatcc ctgttggtaa gctaaacatg gatgaatttg ctatgggtgg aagtacagaa
     6901 acttcttatt tcaagaaaac aaaaaatgct tgggacccaa caaaagtacc tggtggctct
     6961 tctggtggtt ctgcagcagc agttgcagct ggacaaattc cagtttcttt aggtagtgat
     7021 actgggggaa gtatccgcca accagcagct tttaacggca tcgtagggat gaaacctact
     7081 tatggccgtg tctcacgttt tggtttgatt gcctttgctt catctcttga ccaaatcgga
     7141 ccaatgaccc gtacggtgaa agataatgca ttagcattga cagctatcag cggttatgac
     7201 gaaaaagacg gtacttcttc aggcgtttca gttcctgatt ttgcagaggg actgacaggc
     7261 gacatcaaag gaatgaagat tgctttacct aaagaatact taggcgaagg tgtggcccct
     7321 ggcgtaaaag aagctgtatt gaaagcagca gaaacattcc gttcattagg tgctgttgtg
     7381 gaagaagtca gcttgcctca ttcaaaatac ggtgtggctg tttattatat cattgcctca
     7441 tcagaagcaa gttccaattt gcaacgattc gatggtatcc gctacggtta tcgctctgaa
     7501 aatgtgaaat cattagatga tgtatatgtc aactcacgtt cagaaggatt cggtgaagaa
     7561 gtaaaacgcc gtatcatgtt aggtacattc tccttaagcg cgggttatta tgacgcttac
     7621 ttcaaaaaag caggacaagt ccgtacattg atcaaacgag attttgaaaa agtctttgca
     7681 gattatgact tgatcatcgg gccatcttca ccaacagttg cattcggaat cggtgagaac
     7741 attaacgatc caatcacaat gtacatgagt gacatcttaa cgatccctgt caacttggct
     7801 ggacttccag gtatgtctgt accggcaggt ttatcagaag gattaccagt tggattgcag
     7861 atcatcggta attattttga tgaaaaaacg atgtatcaag cggcatatgc gtttgaacaa
     7921 gcaacagaat tccataaaca acaaccagct attttaggag ggaaagacga atgaattttg
     7981 aaacagtcat tggactagaa gtccacgtcg agttaaaaac caactcaaaa attttctctc
     8041 cagcgccagc tcattttggt gcagaaccaa acagcaatac aaacgtgatc gactggggtt
     8101 atccaggcgt gctgccagta atgaataaag ctgcgctgga attcgggatg aaagcagctt
     8161 tggcattaaa ctgtgaaatc tctaaagata ctcattttga ccggaaaaat tacttttacc
     8221 cagataatcc aaaagcatat caaatttccc aatttgatca accaatcggt catgacggat
     8281 ggatcgaaat cgaagtagaa ggtaaaaaga aaaaaatccg gatcgaacgt gtccatttag
     8341 aagaagatgc tggaaaaaac atgcatggta ttggcggtta ttcttacgtt gatttgaacc
     8401 gacaaggcac gcctttgatc gaaatcgttt ctgaagctga tatgcgttca ccagaagaag
     8461 catacgctta tctagaagca ttgcgatcaa ttatccaatt tacagaagtc agcgatgtga
     8521 aaatggaaga aggctctatg cgctgtgatg ccaatatttc tcttcgccct tatggacaag
     8581 aagagttcgg tacgaaagca gaattgaaaa acttgaactc tttatctttc gtgaaaaaag
     8641 gtttagcttt tgaagaaaaa cgtcaagcaa aagttcttct ttcaggagga gaaatccaac
     8701 aagaaacacg ccgttttgac gaaacaacga ataaaacagt tttgatgcgt gtcaaagaag
     8761 ggtctagtga ttatcgttac ttcccagagc ctgatatccc gaaattcgtg atcgatgacg
     8821 aatggattca aaaagtaaaa gctagtttgc ctgagatgcc agcttctcgt cgcgaacgct
     8881 atatccgtga gtttggactt ccagagtatg acgcaatggt attgacactt acaaaagaaa
     8941 tgtctgattt ttttgaagct gttttggaaa atggtgcaga tgccaagcaa gcatctaact
     9001 ggttgatggg tgaagtttct gcttacttga acagtgagaa actggaatta gctgagacaa
     9061 aattgacacc tgagaactta gcaggaatga ttcgtttgat cgccgatgga acgatcagct
     9121 caaaaatcgc caaaaaagtc ttccgtgaat tgatcacaaa tggcggagaa gcccaagaag
     9181 tagttgaaaa aaatggttgg gtgcaattat ctgatccgaa caaactactg ccaatcatta
     9241 atgaaatttt ggataacaac caacaatctg tcgatgattt caaaaatgga aaagatcgtg
     9301 cggttggctt cctagtcggt caaatcatga aagcaactaa aggacaagca aatcctggtg
     9361 tcgtgaataa gctacttaaa gatgaattag cgaaacgata gaggctgaaa atgaaaaaag
     9421 cacgcgtaat ttataacccc acctcgggaa aagaattatt gaaaaaaaat ttggcggata
     9481 tcctacaggc attagaagaa gcaggatttg aagccagcgc ctatgcaact acaccagaac
     9541 cagattcagc caagaatgaa gcatgtagag tcgcagaact aggttttgat ctagtagtgg
     9601 cagctggtgg cgatggcaca atcaacgaag tggttaatgg aatcgctccg cttgaacacc
     9661 gtcctaaaat ggccatcatc ccagctggta caacgaacga ctatgctcga gcattaaaaa
     9721 ttcctagaga taatattaaa gcagcagcag aagtgataaa aaaacaccaa accgtaaaaa
     9781 tggacatcgg cctttctgat aacagttact tcatcaatat cgctgcaggt ggctatttga
     9841 cagaattgac ttatgaagtt ccatcagaac taaagagtat ttttggttat cttgcttatc
     9901 ttgcgaaagg agcagagatg ctccctcgag taaagccaat caaaatgcgc ttgaagtatg
     9961 atgaagggga atatatcgga aatgcttcga tgtttttcct agggctgacg aactctgttg
    10021 gaggattcga acagatcgcg ccagatgcca aattggatga tgggaagttt tctttaatca
    10081 ttgtgaagac agcaaatatc tttgagatct tgcatattgc agcactcatg ctgaatggcg
    10141 gtcgacatgt cgatgatcca agaataatct ataccaaaac aagcagtttg ttcgttgaaa
    10201 cagaagatga tatcaatcga ccagtaatga tcaacttaga tggtgaatac ggcggggaag
    10261 caccgatgca tttccaaaat cttcatcaac acattgagtt ctttgccaat accgatcaga
    10321 ttcctgatga agcaatcaca ggtacagatg aagaagaatt agaggttgtc agcaaagaat
    10381 tcgtaaaaga agtcgaacgt ttaacggatg aagatatcga cggtgacgga aaaatagcag
    10441 acgaaacacc taatcaaaag aattaatcaa aagccggtgc tgaacgtagt aaggtcagtg
    10501 ccggttttag ttgtgtaatt agctgattta tagtagagtt cagtaggatt gaaaaatagg
    10561 cttaaaggag caaaatatga cagaacagca aatagtaaag aaaaacgaac gatatacagt
    10621 cgaaatcgtc gacttgagtt atgaaggaat gggggtagca aaaatcgatg gttatccatt
    10681 atttatcgaa aatgcactcc ctggagaaga agtagaaata ctagtagtaa aagcaggcaa
    10741 taagtttgga tatggaaaag tcgaaacatt tattacacaa tcacctgatc gtcagcctgt
    10801 agaaaacaat gcattacttc gcataggtat cgcaccttta gcccatttga agtatgaaca
    10861 acagcttgtc ttcaaacaaa aacaagtaga gcatgtaatg gcgaagattg ctaagatgcc
    10921 agaagttcct gtacttccaa cggtcggaat ggcagaacca ttcggctacc gtaataaagc
    10981 acagatccct gttcgccgta tcgatggaca gttagccacc ggtttttaca aaaagaatag
    11041 ccatgatcta gtccctatcg aagattttta tatccaagat cctgtcatcg atcaagcaat
    11101 caaagtagtc agagatatct tgcaacggtt ccaagtacga ggatataatg aagaaaaaaa
    11161 tgaaggacag cttcgtcaca tcattatccg tcgtggtcat tattcccatg aaatgatggt
    11221 cgttttagtt actcgtaaag aaaaattctt taaatccaag gaaattgctg aagctattca
    11281 tgaagaattg ccagaagtgg tttcggtcat ccaaaatatc aatgaagaaa aaacaaatgt
    11341 gattttagga gacaaagaaa aagttttata tggcagagac tatatcgaag atcagctatt
    11401 aggaaaaacc tatcgaatct cagcaaagtc attttatcaa gtaaatactc aacaagcaga
    11461 gtatctgtac caaacagcca tcgagtttgc cgagttagat aaaaaagaca ttgtgatcga
    11521 cgcttattca ggaataggta cgattggctt gtctttagca gacagagtgg ataaggtgta
    11581 tgggatggaa gttattccag aagcaattga agatgcacag ttcaatgctt taaataataa
    11641 aatcgaaaat gttcattatg aagtcggaaa agcagaaaaa gttatgaaaa aatggcaaga
    11701 aaaagggatc aaaccatctg tgatcgtagt tgaccctcca cgaaaaggat tagatcatca
    11761 ttttattgaa tcagcaatcg aaatgtcacc agaacgaatc gtctatatct catgtaatcc
    11821 ggcaactttt gcaagagatg ctaagttatt tgcagaagca ggttatgccg tgaagaaagt
    11881 tcagcctgta gatcttttcc cgcaaaccca tcatatcgaa ttagtcgctt cattcgttaa
    11941 aggagaataa aaatggacca aaaagaaaaa atgaaagaat tgatcaaaaa gaaacaaggt
    12001 ggaggccgat ccaaacaaat ggaaacacct aagaatgatc gtaaaaacat gagaaaaggc
    12061 cctaaaatct ttaataaata gacgtgctaa agaaagacta agaagtgtta acttttgtta
    12121 ttgtttctta gtctttttag ttagtctaca ttattttggc gatttgccaa taaggactgt
    12181 tgagttgtgc aaaatcaaat ctgggtcaga atgaacgtca ttctgtctca gatttttcgt
    12241 tatttcaatt tgattaactg attgatcaaa aagattttta ttttcatatt ctcaaatgat
    12301 tcaacactaa atatacatgt ttgacgcgga aattattatt cgattttggc ggcatcaggg
    12361 gggatgacga cgtagcagac ccattatttt actcaagaaa atgtccgtgc ctattattta
    12421 gaatacgata gtgaacgtgc tgggagtttt gttcttttag agaaagtctc tgaagataaa
    12481 ttagttgtct tagattttga ctgtaacgaa aaatggcgaa atggaagagc accagcaagt
    12541 gattgaccgg atctacgaag cgacacgata tctgcctttg gatcgtttgt gcttaagtcc
    12601 tcaatgtgga tttgcttcaa cagaagaaga aaatgttttg tttgaagaac aatggaaaaa
    12661 atttcttttg tcaaaagcat tgcagaagaa gtttggggaa aatcattaaa tcatctagga
    12721 gagcttgata ggaaataaca ctatggtata atcagaatgc ttaatactaa ctactgaggg
    12781 agccttatga aaaagaaaat tcttaaattt atcggatggt tcttcggaat cattcttggg
    12841 atcgcgtttg caatctttct tgccttccag ctttccccca gacctggagc attcttgatc
    12901 aaccatatgt tttcaaatac tgttcaaatc actgataaaa aaacgttcga tcaagcagaa
    12961 aaaaacgtgg cgaaaaaaac agatttgacg tatgagtcca agtataaaga gaatacttat
    13021 gacatctact atccaaaaaa cagcaagggg cctgttcctg ttctattttg ggtacatggt
    13081 ggaggattcg tcggcggaga taaatcgggt gtcaaagaat ttgcgacaaa attagtttca
    13141 gacgccaaca tcgcagtggt tgcgatgaat tatgaattag cgcctgattc agaataccct
    13201 aatcaagttc ttcaagtgaa tgaactgatc aaagatttat tagctgaaga aaaagacgac
    13261 aaattgctag acatggaaca attatttttt ggtggagata gtgcaggttc acaaatcgca
    13321 ttgcaatatg cagctataca aacgaatgat gcgtatgcta aacaaatgaa aatcaaacag
    13381 ctacttccaa aagatagtct gaaaggaacc atttcttatt gcggacctgt tgatttgaag
    13441 ctaacagcac agcagcattc ggatgatcga tttatgaagt ttttcgtcaa aaccgttgcg
    13501 tggtcgttga ttggaacaaa agattggaaa acgagtgagc aattgaaaga agctagtgtg
    13561 gctgaccatg tttctaagga atttccgcca acgtatataa ctgacggaaa tgcatactca
    13621 ttccaggagc aaggtcaagc attagaaaat cggttgacag aattaaaggt acccgtgcaa
    13681 tccttgtttt atacagatac aaaaaaagag attacccatg aataccaatt tgattacacg
    13741 ctaaaagaat cgcaaaattg ttaccagcag acattggatt ttgtgaataa gtataagtaa
    13801 gaggttgtga aaaagccgtt tcgttttgag tatgaagaaa cgaagcggct tttcacaacc
    13861 tctctttcaa tacctttttg ttcttgggat aaaactttcc ctaacaatca atcgtgcagg
    13921 ataagtcaaa atgattggtt cgtcccgtat accgtcaatt cgctccaatg ccattcgaac
    13981 agctgcttgc ccaattgatt tcgcattgat ttgaacactg gtcaagccag gggagaggtt
    14041 tgcgattgtc tcgatattat caaagcttgc tacaacaatc tcttctccaa tttggatatt
    14101 tttttgtttc aatgcccgat agacaccaat tgccattgga tcgctcccga caagaatggc
    14161 agtaggaaga ggggattcat tttctaatag attagtcgca agtacctcac cttgtttttc
    14221 tgtccagcca cctagatgat aagacatata tttttctaag tgatgatcga tcataaaatg
    14281 ctgataggca cgcatcctct tttcatttat agaaatctgc ttcccaccga attcatccat
    14341 atcgacttta tatcccccga tataggctat tcttttatga ccattttcta agaacaattc
    14401 cagtacggtt ttagtcatcc gttcaagatc tgcatacacc atatcgacct cttgctggat
    14461 gtcagggtta tccacgacga ctatttgcat gttttgtttt aacaaggcgg taatcgattc
    14521 tttcgtaaca gtcccaacaa cgatcaccgc acctaactga tctagatctt cccattgttt
    14581 aggattatca gatagattat agattcgatt catccctaaa tgcagacgtc gagcttcttc
    14641 ttccatccct actcgcaaat gtttgtaata gatatcctgt tcttcgctcg ctttgccgat
    14701 cgtagagatc acaccaatcg aaggcattct ctttttgata tatttacgag tactgatgtc
    14761 ataatccaac gcagcagctg cctctactat ccgttgccga gtagtctgag ccacatggat
    14821 cgtctgatcg ttattcaaca cacgggaaac tgtagcagga gagacatttg cctttcgtgc
    14881 gacatctcta attccaacca tttttacaca ctcctcaaaa aaataaaaaa ttt
//
LOCUS       DACCYH010000067        14872 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.070, whole genome shotgun sequence.
ACCESSION   DACCYH010000067 DACCYH010000000
VERSION     DACCYH010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.070"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            118..1857
                     /gene="yfcC"
                     /locus_tag="IVY85_002019"
     CDS             118..1857
                     /gene="yfcC"
                     /locus_tag="IVY85_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP8045031.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2014..3198
                     /gene="iadA"
                     /locus_tag="IVY85_002020"
     CDS             2014..3198
                     /gene="iadA"
                     /locus_tag="IVY85_002020"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP8045032.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3309..3512
                     /locus_tag="IVY85_002021"
     CDS             3309..3512
                     /locus_tag="IVY85_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8045033.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3613..4275)
                     /locus_tag="IVY85_002022"
     CDS             complement(3613..4275)
                     /locus_tag="IVY85_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8045034.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4288..5790)
                     /locus_tag="IVY85_002023"
     CDS             complement(4288..5790)
                     /locus_tag="IVY85_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8045035.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6338..8017
                     /locus_tag="IVY85_002024"
     CDS             6338..8017
                     /locus_tag="IVY85_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8045036.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8144..8623
                     /locus_tag="IVY85_002025"
     CDS             8144..8623
                     /locus_tag="IVY85_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP8045037.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8842..9522
                     /locus_tag="IVY85_002026"
     CDS             8842..9522
                     /locus_tag="IVY85_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP8045038.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9530..10861
                     /locus_tag="IVY85_002027"
     CDS             9530..10861
                     /locus_tag="IVY85_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8045039.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10876..11838
                     /locus_tag="IVY85_002028"
     CDS             10876..11838
                     /locus_tag="IVY85_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP8045040.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11949..12287
                     /locus_tag="IVY85_002029"
     CDS             11949..12287
                     /locus_tag="IVY85_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP8045041.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12366..13073
                     /locus_tag="IVY85_002030"
     CDS             12366..13073
                     /locus_tag="IVY85_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP8045042.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13176..14657)
                     /locus_tag="IVY85_002031"
     CDS             complement(13176..14657)
                     /locus_tag="IVY85_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8045043.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4711 a         2553 c         3184 g         4424 t
ORIGIN      
        1 aaaaagtgat tttctaataa attattcatg tggaaatgtc atttttactg tataattcat
       61 ttgattatcc attttttatt catcaaaatt actgaaaaaa gaaaggaaga aataaatttg
      121 aagaaaacaa attcttcagg aaaaccgaaa cgcgacttca ctaaattatc gacaccccat
      181 acgtatgtca ttatttttgg tgtagtcatc tttgcatgga tactgacttt tgtcgttcca
      241 gcaggaaagt tcagcacgca agatattgaa tacaaagatg caaatggcga aacaagtact
      301 cgtactgttt tacgtcaaga ttccttccgt tatgcttacg agctggataa gtcgtatgtg
      361 tttgatcaat tagaagaact gcaagatcat ccagcagaac gtgaaaaact ggatgttccg
      421 gaaaaaggat tggaaaaagt cattgctgac ggagaaaaaa atcttacgca ggaaaaacta
      481 gatgaaatct ccttgacaga tgatgtgctt tacgatcagt acggcgaaaa tatctatgat
      541 acttctaaaa aattgcataa aacagccaaa atatggggaa cagatgattt tggcggcttt
      601 ggtttcttaa attttgtttt tgaaggacta gtatctggag ataagtatgg atctgctgta
      661 gggatcgctg cattgatcct tgttgttgga ggtgcttttg gtattattat gcgtaccgga
      721 gcgatcgacg caggtatcta tgcgtttatc agtaaaacta agggactgga gcgtttagct
      781 ttaccgctat tattctttgc tttttcattc ggtggcgcta cattcgggat ggcagaagag
      841 gtaattccat tctcaatggt gatggttcca tttgtcattg cgttaggata tgactcgatc
      901 gtagcagtaa ccgtgactta tgtcgcatct caagtcggaa atgcgacctc ttggatgagc
      961 ccgtttagcg tagcggtggc tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca
     1021 ttccgattga ttatgtgggt agttgttact gcattagccg ctggttatat gatgatatat
     1081 gcagaaaaaa tccgtaaaaa accagaaagt tcgttgactt acaaatcaga tgattatttc
     1141 cgttctcata tcaaaaagac atctgatgaa aataaaaaat tcatgcttgg gcataaattg
     1201 atcttattag agatgttagt tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc
     1261 tattacattc cagaaatcgc ttctcaattc tttgtgatgg gattagttgc tggaatcatt
     1321 gctgttcttt tcaaattaga tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct
     1381 ggtgcagctg atttagctgg tacagcaatc gtagtaggta tggccaaagg gattcttctt
     1441 gtcttgggag gatctgatgc atctgttcct tcagctttga atacgatttt gcatgggatc
     1501 gggacggcgt tgaccggtgt tccagctgtc attggtgctt gggcgatgta catcttccaa
     1561 agtttgttca acttggttgt tacatcaaat tcaggacaag cagctttgac tatgccaatc
     1621 atggctccac tggcagattt agtaggtgta tccagacaag tagcagttct tgcttatcag
     1681 cttggttcag gttttatgga cgcttttaca cccgtatcag ccagcttgat tggtgtgcta
     1741 ggtgttgcac ggatcgaatg ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc
     1801 ctgtttgttt taggaactat tttcatcatg attgccattg caataggtct gcagtagtac
     1861 tggagacctg taaataaaaa agtaatccaa acagtagata aggactccaa gaaccgtcat
     1921 tttacggtaa aatcaaagga gtaaagatcc tatcgtttgg attatttttt aagatcctat
     1981 catcagttat acttaaagaa agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg
     2041 tttgcaccag attacttagg aaaaatggat gtactgattg ccggtgggaa gattttggct
     2101 gtagaagaac agctagaagg cggctacgaa ggagtggaag tagaagaact ttcaggtgag
     2161 ggaaaagtac taaccccggg tttcattgac tgtcatttcc atattttagg tggaggagga
     2221 gaaggcggtt atcaaaatcg aacgccagaa gtcacattga gccaattgac tacagctggt
     2281 gtaacgacag ttgtcggttg tttgggaaca gacggagaag gtagagatat gactgcgttg
     2341 atcagcaaag cgaaaggtct ggaagcagaa ggaatctcca cttatgtata cgaaggttcg
     2401 taccgattgc cggtaaaaac agtaacagat tctatcatca aagacttttt gacaattgat
     2461 aaaattatcg gaatcggaga aatcgcagta tccgatcacc gttcttctca gccaagtttt
     2521 gaagagttcg ctcgtgcagt agcagatgct cgtgtaggag gcatgttatc cggaaaagct
     2581 gggatcatca atgtccactt aggcggagga aaaagaaagc tagagctatt gacacgaatc
     2641 gtagaagaaa cagaaattcc gatcacacaa tttttgccta ctcatgcaaa tcgcacacca
     2701 gaattatttg aagcttgcgt ggcatttgcc aaacgtggtg gcacgattga cttcactgct
     2761 agcgaagatc ctgatttttg ggagaagaca gatggtgaag ttcgcttcag caaagcactg
     2821 aaacgattga tcgaggaaga tgtttcttta gataatttca cgatgacctc agatggacaa
     2881 ggaagcttgc cttattttga tgaaaacaat catttcctag ggttaggagt aggaagtgcc
     2941 aaggcattgc tagtagggat caaagaagct gtgcaaaaag aaagtatccc tttggaaatc
     3001 gccttacgag ctataaccag taatccagca cgtatattga aattggataa aaaaggaaaa
     3061 atcgaaattg gtgctgatgc agatctgtgt atcttggata aagaaacgct tgatattgat
     3121 acagttatcg ctaaaggaga aataatggtc caagaaaaag aagtcaaagt atgggggact
     3181 ttcgaaaaaa gtttttaatc aacagctcta actatctttt ttagaaaacg agcgttctgt
     3241 ttgtctgtat ctgttgttga tgttaccctg aaggtagatc aataaacaaa ctaagaggag
     3301 gaagcacaat ggcagattta aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg
     3361 aaggaaaaga agcatacggc aaagcaacag acgacaagag caaagaaaca gaagggaaag
     3421 cacaatcact aggtgctgac atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag
     3481 acaagttttc tcgaaaagac gaaaaaaaat aaaaaccact tattactaat tgaatcaagc
     3541 aaataaaaaa tccgaaccat atctgcgaga cagatatggt tcggattttt tatttgatca
     3601 ttatcctgtt ttttaaactt cgattttttg aaatttttta ttttttagtt caaagacttc
     3661 atcacagctt tttgctactt caatctcatg agtcacgatg ataatacagc gattttgttc
     3721 atgggcgatt tcttggaaaa gtttgacgat atcttgtgaa gttgtttcat ctagatttcc
     3781 agtaggctca tccgctacga ttagttgatg ttcacagcag atagcacgaa cgatagcgac
     3841 tcgctgctgc tgacctcccg aaagtttggt cacctgtttt tttgctaata tttcatcaat
     3901 tcctacacga gccagtcgat ctaaagcaac ttgtcgataa ttctcttgct tggtcttagt
     3961 gatttccatt gctgctatga cgttgtctag agccgataaa taaggaagca agttgtaagc
     4021 ttggaaaata atcgagacat catttttacg ataacttctt aacccgattt tgttcagtga
     4081 acgatcttga tagtatacct ttccttcttt tggtgcatct agtccagaaa taagcgaaag
     4141 aaaagtcgtt tttccagaac cacttgctcc taaaatagcg tacatttttc cagcttcgaa
     4201 attcagttct acatctttat aaagcgcatc ttgcggatct tgataccaaa atcccaattg
     4261 ttctgtccgt aacatcaaat tcctccttta gtttaatagg atttttcttg ggtttaatct
     4321 taatatccct atagaagcta aaaagacaga aaacagactg attccaaatg ctactccggc
     4381 taatagtccc aattgtgcag gtttgacatt gatttccaat tcgctgactt gatcagaaac
     4441 agcaaatgga ttattcgtgg ttccttgttg accatcgcgt tgaccgcctt gttcttgtct
     4501 gttacctggc atttgtccac cgccaggagc ttgctggttt tgtgtcgtgg tttctgtctg
     4561 ttggtcaagt agctgctgtc ccacggcatt cgcgacgacg tttccgctaa gcgaagcaac
     4621 tcctaatgca agaatcatac aaatagccac ttcggtaaac aattggagaa tgattttact
     4681 tctggattct cctaaagata gcaagacgcc taactcatgc cgacgctccc gaatggtaat
     4741 cataatgatc agagtaagta tgataatacc ggctacagcc actagtaaaa caacattttt
     4801 agaaaaactt gccacgttgt ttaaaggttc caacattgac tgatacatgg aatcatttga
     4861 ttgtaagcta aatgtatctg tgtcgatcag cttctcagct tctttgagaa actcatccat
     4921 tttatctgga tcagatagag tgtaaatagc agaatctatc gtgttatcgt cagatgtccc
     4981 atttagttca tttgcaaaag tgtaagaagt gtaaagtgta ttcgacggat tcatgaagtt
     5041 gaatttcata cccatactgc cgcttgtttc gctggattca taaattcctt tgatcgtcat
     5101 ttcataagtg gtatcttcat cttcagtact cgtgattgtg aaagtatctc cgactgccag
     5161 gtcatttgtt tctgctagag tagaatcaat gactacattg tttgtgcctt tgtcgctgtc
     5221 atctattcct tcaccatccg tgatttttgc tgttccatca gaaaaattac tattttgtgc
     5281 agttgctgac acaccactga tttgaaagtc tgcttgagtc atttgcggat tcccgcccat
     5341 tcctccagca ggttcaccgc ctgcatcagt agtttctgaa ctgtcttcac ttgaatcaga
     5401 actagtgatg gcagttattc catcttttgc catagctgag gcggaacttt caaaggaata
     5461 actctttaca ttatctaatt cagctatttt ttccgcgtcc gttaaggaaa ctggtgtaag
     5521 agaaaaactg cctggatctg gacgttcttc agtggtagaa ccgcttgaag aagtttgttc
     5581 ctgtttttta aatgaagctt ctcgattggc acttaaagtc actgttgctc caacttcttt
     5641 ttgtgcttga ttcgctgctt cttctgcagc gcttcgaatc gtgaggccag ctagcacaaa
     5701 aataagaatg gctgtgaaca cggcaaacaa taacaatgaa cgtccccatt ttacttttgt
     5761 acttttccat gcacgtttca taaaattcat attttgcatc cctcctagta gatgcatttg
     5821 ataatagaca acctatctta aagctagctt aaatcttatg ctgtttgcac accatcaaca
     5881 tgaggttctg tatatataaa aagtcactat agttattgca aaaaatgtgg taaaatgaaa
     5941 aaagtatgtt tcaaaccgac gaagaagaaa tgaaagagga atagcaaaag aaatcctaga
     6001 tgctacagag ggatttttac gtgcatagtt gttctaacaa ttgctgatac tagcgattta
     6061 gtttatttgt tgtataataa tggggaacag ctgaatactt atataagtat tctggctata
     6121 gcgtttcgca aaagatccat tttgttaatg aagtatatcc aaaataaaat gcccatccaa
     6181 ctgtcagaac gcggatgtgc tggtaatttt cctgctgatt ctccataagt tgagaaatag
     6241 tgattttgca tatgcttcat tggaatggct catatattta gataaagaag aaaatcgaca
     6301 tctttaaact tggatgtcat aagaagaggt gaagtttgtg agtaccatta aaattatccc
     6361 attaggcggc gttcgtgaaa atggaaaaaa catgtatgtc gcagaagtgg gcgaggatat
     6421 ttttatttta gattgtgggc taaaatatcc tgaaaatgag ttgttgggga tcgacgtcgt
     6481 aatccctgat tttacgtatt tagaagaaaa cattgatcgt gtagctggtg tctttttaac
     6541 acatggacat gcagatgcga tcggggcact tccttacttg ttgtcaaaaa tttctgtacc
     6601 agtattcggg acagagttga cgatcgagtt agcaaaattg aatgtcagtc gtaatgaagc
     6661 agccaaagga tttaaggatt tccatgtggt agatgagcat acagaaatag actttggtga
     6721 aacagtagtg agctttttcc gtaccactca tacgatacca gattcagtgg ggatcaatct
     6781 gaaaacaaaa gaaggtagta tcgtttatac gggagatttt aaatttgacc aaacggctat
     6841 cccaatgtat caaacagatt atgcacgatt ggcggaaatc ggcaaagaag gagtcttagc
     6901 cttactaagt gaatcggcaa atgcagaaaa tcctcagcct gtagcttctg aagcccaaat
     6961 agcagatgaa gtatttgata cgatcaaata ttgggaaggg cgtatcatcg tcgcttgtgt
     7021 agcaagtaat ttgcaacgag tgcaacaagt attggatgca gcagaaaaag cacaacgaaa
     7081 agttgtcttg actggtcaag atttcgaacg aattattcgc acagcgatgc gtttagaaaa
     7141 acttcaatta ccaagtgaag atctgcttgt tacgttgaaa gacatgaaaa actatgatcc
     7201 agaagaactg atcattttag aaactggccg aatgggtgaa cccatcaagt ctctgcaaaa
     7261 aatggcaaac ggtacacacc gtaatgtcaa gattgaagaa ggagatttgg tttatatcac
     7321 tactacgcca agcatcgcga tggaaaccat cgtggcgaaa acggaagata tcatttatcg
     7381 agctggtgga acagtgaagt tgatctctga gaatatgcgc gtttctggcc atgccaatcc
     7441 taatgacttg caattaatga tcaatttgat gaaaccaaca tattttgtgc cagttcaagg
     7501 agagtatcgt gaattagctg ctcatgcaga tttggctcat gcagtaggga tgccatacaa
     7561 aaatatctat attactggac gcggagacgt gctggaatat aagaacaagc gtatgagtgt
     7621 tgcaggaact acaagtgcag aaaatgtaat gattgatgga ttaggcatag gtgatatcgg
     7681 aaacattgtt ttacgtgatc gaaaaatctt gtcagaagac gggattttcg tagcggttgt
     7741 cactatcagt cggcgagaaa agaaaatcat ttcaaaacct caaattactt cacggggatt
     7801 cgtttacgta aaagctagcc gtgatctgat gaaagaaagc agcaatatga ttgaaacaat
     7861 cgttgagaaa catttagaaa gcaatgattt tgagtggagc aaattaaagc aagacatccg
     7921 agatcaatta ggccgttact tatttgaaca aacaaaacgc cgcccagtga tcttgccagt
     7981 cattatggaa gctactcaac gtcgaaataa aaaataaaca aaaagagtaa gatgaacctc
     8041 tctatagagt gctcgtcttg ctcttttttt gcgaaaaaca aataattttt gaatacagca
     8101 tccctcgctt tataataaac gatagaataa agggagtgaa tcaatgatga gtcaaacaaa
     8161 agtcgaacgt tatttagaag aacaaaagat cagctatcaa ccacttgatt ttagtcatgt
     8221 ggtagaagaa tctttcacag aagaactaca aaaaagaggg attgatccag ctttgatatg
     8281 taaaactctc gtagtgaaag gaaacaaaac tggggtgatc atcgcattga ttcctttgaa
     8341 cgatcactta gattacaaaa aaacgagaca agtgacgaaa gatcgtaaag ttggttttcc
     8401 tggaatggat tttgtattaa tgcatacagg atatccccac ggagcaaaca cccctattgg
     8461 aataaaactc gcacatcctg attattgttt tttagcagac agttctttaa aagaaaaaga
     8521 agaagtaatt gtgtcaagcg gtgaaatcgg gagaagcctt cggataaaag tagccgattt
     8581 agaaaagatc gtccatccta tttacgctga tttaattagt taagggacga gaagagaagc
     8641 taagcagagc agagacctca aacttgagat tatatctgct tggtacttgg tgcatcctgt
     8701 attatctgtt acaattgctt tgtacatact gtactatcaa tccaatgaaa atatatttag
     8761 cagctatatt ttttcattga tgtgcaagtg atgaaatcgt ttttaataaa aaagatacag
     8821 agaaaacgcg aggtgtaaaa tatgaaaatt acgtatcatg gacattcctg tttaacaatt
     8881 gaaatggaaa atggccaacg cgtgatgatt gacccgttta ttacaggaaa tccattgaca
     8941 gacttaactg cagaagaagt aaagacagat tggttgctga ttactcatgg tcatagtgac
     9001 cacataggag atatgattcc tattgcaaaa gaaaatcaag ccacaattat cagtattgtt
     9061 gagattgctg attatgcgaa cagcagagga gtagattcat tcggtatgaa tatcggtgga
     9121 aaacatgcgt ttccattcgg cgtggtaaaa ttcgtacatg cacagcatag ttcaagctat
     9181 gaagtagacg gtatcgttca gtatatgggc gagcctagcg gaattattat tcaagcagaa
     9241 ggaaaaacga tttatcatgc aggggatact gcttatttca gtgatctagg attgcttgca
     9301 gaagaattcg atattgatgt cgctttcttg ccgattggcg ataactatac gatggggcca
     9361 gaagatgcag caaaggctgc gcaaacaatt cgtgccaaga aagtagttcc aatccattac
     9421 aatactttcc ctgtcattga acaagatccc gaatcttttg tagagttact ggaagatagt
     9481 gaaggaaaaa taatgagcgt ctcggaaacg ctggaagtgt aggcagaaaa tggcgacaaa
     9541 acatgatttg attttagaat atattgaaag tttacctgtt ggcaatcgaa tttctgtaag
     9601 aagcatcgca aaagaattga atgtcagtga aggaacagcg taccgtgcaa tcaaagatgc
     9661 agaaaatgtc ggactagttt caacaattca acgagtaggg accatccgta tcgaacgcaa
     9721 attaaaaaaa catattgaac gtttgacatt tggcgaggtc gtcaggatca ttgaagggga
     9781 cgttttaggc ggggcggcag gattagacaa ggtcttgaat aaattcgtca tcggcgcgat
     9841 gactaaacat gccatggaac gctatattac gccaggatca ctaatgatcg tcggcaaccg
     9901 ggcggaagtc caaaaattag cattggaaga tggcgccgca gtattgatca caggaggatt
     9961 caatacgact gctgagattg caaagctagc tgatgatctt gaaatgccga tcttacgcac
    10021 ctcttatgat acatttacag tcgcaaccat gatcaaccga gcgttaagcg accaattgat
    10081 caaaaaagat atcatgcttg tcagtgatat ctatatgcca gctgaaaaga ctcattattt
    10141 gcagcagaca gatacgattt ctgattatca gcgtttgtca gaagaaacgc agcattcgcg
    10201 ttttccagtc gtcaaccgcc atcatcgttt gatggggatt gttacggcaa aagatgtttt
    10261 agggaaatcg ccaaaccaga tcatcgatcg ggtgatgacg aaagaaccaa tcagtgtaaa
    10321 gaaaacaatg agtattgcct ctgttagcca tcaaatgatc tgggatgggc tagaagtcat
    10381 gccggtcgtt tccgatgact tgacattgga aggcttgatt acccggcaag atgtaatgaa
    10441 agcgatgcag cttgtccagc gacaaccgca gattgcagat acgatttcgg atcagatatc
    10501 tggaaatatc catacgattg atactgaccg tgaaggaaat cgtttggaaa accctaagtt
    10561 caaattcgta gtagcaccac aaatggtcaa tggcgttgga accatttctt ttggcgtact
    10621 tagcgaaatc atttctgatg tcgcacaaaa aacaatggtg atgaaccaaa aacgaaatat
    10681 cttgattgaa caagtgaacc tacattattt gcggctgatc caattggaaa gcgaattaga
    10741 cattcgtcca cgtgtattgg aaatcggcag acgttccgct aaactggata tcgaagtgtt
    10801 tattgaaaac accatcgttt ccaaagcaat cgttgtttgt caagtaatgg aacgttctta
    10861 gaaagcagga gtgaaatgac agtacagcaa gaaatagtag atacaataaa aaaatacgat
    10921 aaaatcatta tccacaggca tttgcgccct gatcctgatg cattaggatc acaagtagga
    10981 ttagccgaac tattacggga aagtttccca gaaaaagaaa tacgtcaagt aggtgaaacg
    11041 attgaagggc ttcgcttttt agcagaaatg caagaagttg aagatgtctt ctaccaagga
    11101 gcgctagtca tcgtgactga tacagctaat tcacccagaa tcagtgatca aagatatcag
    11161 ttggcagaaa aactgatcaa aatcgaccat catcccaatg atgaccctta tggagatctt
    11221 gtgtgggtaa atacaaaagc aagtagctgt agtgagatga ttgcagaatt tgctttgatg
    11281 tttcctgaat ctttaaagat gaacgttaag gctgcacggt tactttatgc aggaatcgtc
    11341 ggagatacag ggcgcttttt gtatccctcc acgacgtccc gtacattaga gattgcttcg
    11401 atattgagaa attatccatt tgatgcaagt gctttgaaca gagagattga acaaatgccg
    11461 atgaaagtcg ctaaactttc cggttatctt tatcaaaata tccaggtgga tgaaaatggt
    11521 gcaggaagaa taatccttcc gcaagaaatt cttgataaat atggaatcga tgattcagag
    11581 acagcttcga tcgtttcact tcctggagtg attgacgaag tgctggcttg ggggattttt
    11641 gttcagcagc cttcagggta ttaccgcgta cgtttacgtt ctaaggggcc gatcatcaat
    11701 gaattggcta aacgccatca tggaggaggc catccattag caagtggtgc gaatgcaaaa
    11761 gatttagcag aagtagagga aatctataaa gagatccaag atatatgcca atcgtataag
    11821 agcagaaaaa cagaatgata cttcgttcga aaacgtgcct tttgtagtaa aaatcacaaa
    11881 tgtatctttt caagatcttc tttttccgtt acaatagtgc tcactaaaga ttttaaagga
    11941 gaaagaagat gacattacca aattgcccag aatgtggctc agagtacaca tatgaagatc
    12001 gaggcttgtt gatttgccca gaatgcggga acgaatggaa caaagaagaa caagaaactg
    12061 tcatagaaga cggattagtg gtattagatg caaatggaaa ccgtctgtta gagggcgata
    12121 gcgtgacagt gattaaagac ttgaaagtaa aaggtgcgtc aggtgccatt aaacaaggaa
    12181 caaaagtcaa gaacatccgt ttagtagaag gcgatcataa tatcgattgc aaggtagaag
    12241 gatttggacc gatgaaactg aaatcggaat ttgtgaaaaa gaattaaaga atattcaagg
    12301 tcttttcaca ttcttttcct actactaatg ataaaataaa gtaaacgaaa aggagtgaaa
    12361 cgaaaatgta tggttatgga atgtatggtt ttttagatcc gacatttctt ttggtcattc
    12421 ttggattagt tatttccgga attgcgtcag cgtacgtcaa tagtactttc cgaaaatacg
    12481 atcaagtgag aagcaaaaac aatgtgactg gtacacaagc agcacaatat atattacaaa
    12541 gtcaacagat aaataatgtc ggtgttcaac aaattgccgg agatctcaca gataactaca
    12601 attcaggtaa taagatgttg agtttgtctc aagcaactgc acagtctact tcagttgctg
    12661 cgataggtgt agcagctcat gaatgcggac atgcggtaca agatgcttcg ggttatgtcc
    12721 cattgaagat tcgtgcagca cttgttccag cagctaactt tggttcgatg atttcgttcc
    12781 ccttgattat ggtaggtgta ttgcttagct ggaactctac gttaatcaat atcgggattt
    12841 tcgctttttc tttagcatta cttttccaat tggtgacatt accagtcgaa tttaacgcgt
    12901 ctcggcgagc aatccaaatt ttgagtgaag gcggactact gacggaagaa gaagtaccga
    12961 tggcaagaca cgtattattt gcagcagcac tgacttatgt tgcagcagcg ctttcgacat
    13021 tcctgcagct gttgcgcttg atcttattgt tcggcggcaa ccgaagagat taaaataaag
    13081 aagttgtgaa atcagcgctt tgatctgagc taataccgag agttagctga ttgaatacca
    13141 tttatttgga ataagaggat gtgacaagaa ttttgtcaca tcctctttgt tttgttctca
    13201 taaccttttt tcatatacag ttcatgaacc gcttttatta acttgccaaa taacactgta
    13261 ttgtgcgttg tatccagata gaaagtctcc gtttcatctg aaattttctc atgctgatct
    13321 gtaatgatca aatcagcatg ttctgaatat gcggtaattt taacagcatc tttgcgaaat
    13381 aactgctgca aatggctttg aagatgataa tttcctgtaa actgtttggt tatctgcaaa
    13441 tggatgagta actgctcttt cgtttctgtt tgtagtaagt gttggatcaa ttgactaagg
    13501 tattgactga tttggtttct ttccccattt tttgaaaaaa ttgtatcgat tttttctctt
    13561 aatttctttt cctgtataaa agaactagaa tgattggacg tggcttctgg aaagcaaagt
    13621 gtagaaaaac tactgctgga ttcacccaac agttcgaaaa aaacaactgc caacacaaga
    13681 aaataaaaat aaattggtat tttattttct aaatggttga gaggaaactg ttgagttaag
    13741 taagtcatca attgaccgac ttgtcgacaa ataaggtttt tgcttgtgga aaataaaaaa
    13801 ccaatctcct gtttttgtcg aaaagagatt tgatttggaa aaaaacaata gaaaaaataa
    13861 ttaaagtagg ctctctctgt ttctgtctct tcacaagggc gactcactga aaaagaagta
    13921 aagtattctg aataatcatg tgtatgctgc aataagtcca tcaattcagt gatttcagaa
    13981 gaagtttttg gaaaatatgc ctgttgtttg tttcgtaata tgaataaagt taacagcaag
    14041 tagatatttt tttcttgttt tgaaattttt tctatttgct catctgtact gttcattttt
    14101 agtgcattta attcaagtga ggaaaatggc caatcgttac ctccaaatgt atctgaaaga
    14161 aaaaagtaaa gatagagacg gatagataac tcatttcctt caagagtaca gcaatttttt
    14221 acagatagga tccgtaaatg gaattttatt aaggaatgat tgagctgttt tgtcagtttt
    14281 tcgagatagg agggcgatat ataaagttgt tcaagtaaat atttttttgg tacagcagtt
    14341 tgtggataat tgattagtaa catcaaatag ttaaaacgat tggattcttt taggtagcat
    14401 aattttaact tagaaaaaac agctaacttt ttagtttgat cggtttcttt ggtttggatc
    14461 tctcctttat gattagaata aataaattca tcaatcaata aaaattgctt gagtgataca
    14521 tttatttttc ggatcagttc tttgattgtc cgttttggaa tatgtaagtc attggataat
    14581 tcttgtaaag aacaattatt tttttgaatg accatttgta gaacaagtaa ctcttttttt
    14641 cttgcttcaa ttagcataaa tacctccttt ttattctgtt tttgtatata gttagacaaa
    14701 aactgtgagt ggaaagtcat ttcagtctgt gttattctca cagagtgtcg ccttcttttt
    14761 tatataacgt tatttaattt ttttgttacc ttgtttattc tattgtttat ttcctagggt
    14821 attttgtcaa gatattcttg tttttaaggc ggaaaaattg atttttaatg ga
//
LOCUS       DACCYH010000068        14820 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.071, whole genome shotgun sequence.
ACCESSION   DACCYH010000068 DACCYH010000000
VERSION     DACCYH010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.071"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(296..1024)
                     /locus_tag="IVY85_002032"
     CDS             complement(296..1024)
                     /locus_tag="IVY85_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8045044.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="IVY85_002033"
     CDS             complement(1002..2576)
                     /locus_tag="IVY85_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8045045.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="IVY85_002034"
     CDS             2805..3743
                     /locus_tag="IVY85_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8045046.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="IVY85_002035"
     CDS             3755..4666
                     /locus_tag="IVY85_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8045047.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="IVY85_002036"
     CDS             4690..6162
                     /locus_tag="IVY85_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8045048.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="IVY85_002037"
     CDS             6213..6824
                     /locus_tag="IVY85_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8045049.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="IVY85_002038"
     CDS             6854..8767
                     /locus_tag="IVY85_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAP8045050.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="IVY85_002039"
     CDS             8895..11876
                     /locus_tag="IVY85_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAP8045051.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="IVY85_002040"
     CDS             11873..13534
                     /locus_tag="IVY85_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAP8045052.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="IVY85_002041"
     CDS             13574..14506
                     /locus_tag="IVY85_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP8045053.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4818 a         2333 c         2979 g         4690 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagttgaaa
//
LOCUS       DACCYH010000069        14496 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.072, whole genome shotgun sequence.
ACCESSION   DACCYH010000069 DACCYH010000000
VERSION     DACCYH010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.072"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            202..546
                     /locus_tag="IVY85_002042"
     CDS             202..546
                     /locus_tag="IVY85_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP8045054.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            547..966
                     /locus_tag="IVY85_002043"
     CDS             547..966
                     /locus_tag="IVY85_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP8045055.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1058..1408)
                     /locus_tag="IVY85_002044"
     CDS             complement(1058..1408)
                     /locus_tag="IVY85_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045056.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1374..2705)
                     /locus_tag="IVY85_002045"
     CDS             complement(1374..2705)
                     /locus_tag="IVY85_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP8045057.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2961..3527
                     /locus_tag="IVY85_002046"
     CDS             2961..3527
                     /locus_tag="IVY85_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8045058.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3672..3759
                     /locus_tag="IVY85_002047"
     tRNA            3672..3759
                     /locus_tag="IVY85_002047"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3704..3706,aa:Ser,seq:cga)
     gene            complement(3811..5016)
                     /locus_tag="IVY85_002048"
     CDS             complement(3811..5016)
                     /locus_tag="IVY85_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8045059.1"
                     /translation="MAKKEIDKRIKKHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRG
                     FSTPLAAERALKRLEADIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTK
                     QIFDTHILKVFGDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQI
                     ITSNPMKLVSIPRGEALDIEDKNIEFYTKEELIKFLEAIRNDDDERYLFFSLLAFTGI
                     RKGEAFALTWSDIDFKSKTLNINKTVTRGYQGRLIVNTPKSKSGKRKIYLDNDLISLL
                     RKYYTKNKTIVTIQRENLIFHHDGLLYNPTVSRSWLNVIYKHHPELTKRITTHGFRHT
                     HASLLFESGASLKDVQERLGHADIQTTSNIYTHVTETQNKKVINNFVAFMKNSTQGES
                     KGESKNILG"
     gene            complement(5154..6042)
                     /locus_tag="IVY85_002049"
                     /pseudo
     CDS             complement(5154..6042)
                     /locus_tag="IVY85_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
     gene            complement(6129..6329)
                     /locus_tag="IVY85_002050"
     CDS             complement(6129..6329)
                     /locus_tag="IVY85_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045060.1"
                     /translation="MKIGMRKPSIKKSISARTTGKAKRKVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            complement(6358..6780)
                     /locus_tag="IVY85_002051"
     CDS             complement(6358..6780)
                     /locus_tag="IVY85_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8045061.1"
                     /translation="MYLPQIDNKINKLVKLYQTRNPFRIARELKILVLEEDLGEIYGY
                     YSKFKQIKMIHINSSLDEMGKWITCSHELGHCVLHPNENTPMLSKKTLVSELKIEKEA
                     NYFATHLTIDPTLDGFDHMTKYEKLVCFGLPDEFDRFL"
     gene            complement(6797..7219)
                     /locus_tag="IVY85_002052"
     CDS             complement(6797..7219)
                     /locus_tag="IVY85_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8045062.1"
                     /translation="MTTFERIKELAKNKNKNLQQVAKDLKYGENYFYSLNAGKQPTAE
                     KLQEIADYFDVSVDYLLGRTEKKRYYDLTEKDERDIQKELQSLIDDLSNADGMAFSKK
                     DNEMSEATREALIISLENALRIAKIEAKKKYTPKKYRD"
     gene            7510..7701
                     /locus_tag="IVY85_002053"
     CDS             7510..7701
                     /locus_tag="IVY85_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045063.1"
                     /translation="MSNLDNGRAAIKEFMKENGITMDDLATAYGHTRVRMQQILDGHW
                     SGPKANRTILEIIRDYRIR"
     gene            7712..7978
                     /locus_tag="IVY85_002054"
     CDS             7712..7978
                     /locus_tag="IVY85_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045064.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALSQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLIAHGLKEIRIDGFLRYDKKDIDELMAKYKI"
     gene            complement(8219..8698)
                     /locus_tag="IVY85_002055"
     CDS             complement(8219..8698)
                     /locus_tag="IVY85_002055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045065.1"
                     /translation="MSRKIDNIILLTTIFYLVIGATLSLLSFLSNSSLIVLNQKSILT
                     EFAHIDYFSFFKTGLICLFLSSFILYIGTTFCLMVLNLKMFIIEKWIVIIAKIFSSIS
                     IIIPMTLTFSKEQFNVATTFVSFIALFSFAVPKTFQSKSSNAKVNVENTDRKNDESY"
     gene            8743..9450
                     /locus_tag="IVY85_002056"
     CDS             8743..9450
                     /locus_tag="IVY85_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP8045066.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFDKKHRHVLTAIDELKEGVAEN
                     WADLFWEDTYVHPQNKQSYRIIYMNRDGFSLLAMGFTGKKALSFKLQYIEAFNKMEKE
                     LKDQLPSIPATKREMALLALAANEETNERVDAIESDLNDLKNNQLLAEPDYRTISNMV
                     RNKIRVICNQQHLNSKAKAELFKDLNGGIKRITGAVARNRIKAKQFDDVIDFINNWMP
                     STATMTIIKQMELLEDE"
     gene            9629..9970
                     /locus_tag="IVY85_002057"
     CDS             9629..9970
                     /locus_tag="IVY85_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045067.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKTEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            9963..10709
                     /locus_tag="IVY85_002058"
     CDS             9963..10709
                     /locus_tag="IVY85_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP8045068.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPEDVSMLEPATQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            10715..11401
                     /locus_tag="IVY85_002059"
     CDS             10715..11401
                     /locus_tag="IVY85_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045069.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHNIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            11408..12241
                     /locus_tag="IVY85_002060"
     CDS             11408..12241
                     /locus_tag="IVY85_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HAP8045070.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIEIEKDIDIDK
                     EEKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEY
                     VINWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLP
                     I"
     gene            12258..13109
                     /locus_tag="IVY85_002061"
     CDS             12258..13109
                     /locus_tag="IVY85_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8045071.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSFVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            13106..13465
                     /locus_tag="IVY85_002062"
     CDS             13106..13465
                     /locus_tag="IVY85_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP8045072.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            13480..13641
                     /locus_tag="IVY85_002063"
     CDS             13480..13641
                     /locus_tag="IVY85_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8045073.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERIKAVMVNETARKAINLHIVNL
                     RREIRRLEE"
     gene            13638..13943
                     /locus_tag="IVY85_002064"
     CDS             13638..13943
                     /locus_tag="IVY85_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045074.1"
                     /translation="MSVGKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            13943..14257
                     /locus_tag="IVY85_002065"
     CDS             13943..14257
                     /locus_tag="IVY85_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP8045075.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKAKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYREILNEYFE
                     ED"
BASE COUNT         5067 a         2336 c         2804 g         4289 t
ORIGIN      
        1 tgtctcgctt aattatacaa acggcgtaaa ataaaaaaag cgttagcaac tatgcttaaa
       61 aagcatatcg actgaataaa ctaagcattg gacaggctga aagactacat ttaaaatgaa
      121 atgcgaagaa acaatagaaa ataaagttgc cttagaacca tagttgaatc acctaacagt
      181 cgtgaaggga gaataagaga aatggcagaa aaacaaaccg caggacgaga tcaattagga
      241 gattttgcac caaaatttgc agaattgaat gatgatgttt tatttggaca agtatggtcc
      301 agagaagaat tatctgctca tcagcgtagt atcatcacca tatctgcatt aatttcagga
      361 ggaaattttg aacaattgcc agctcatctg aagattggca agcaaaatgg cattactagt
      421 gaagaaatag cagaaatcat tacccattta gctttttatg taggatggcc aaaagcttgg
      481 tcagctttta gtttagcaaa agaaatctat ttagaagaaa atagcgaaga taagaaagag
      541 gcgtaaatga cgacaaaaca cgaagaaata cacaatggcg tgatttttcc agcaggtgaa
      601 aaaaacgaag cttacgctca agtatttacc gggcaaagct atctgaatat gttagtgaat
      661 gatcccgaag tttctgttgg tgtaggtaat gtaatttttg aaccaggttg tcgcaatcac
      721 tggcatgtcc ataaagacgg ctatcaaatc ttacttgtga ccggaggaga aggctggtat
      781 caggaagaag gaaagccagc aagacgatta atggctggag atgtcgtcgt aacgcacaaa
      841 gggattaaac attggcatgg tgcgacaaaa gacagttggt ttgaacattt ggcgatcact
      901 gctggtaatt cagagttttt agaacctgta acagatgaag tgtatgaagg attggaaaat
      961 caataatgtc aagcttgaac agctcctgaa agtagatttt aatcgaactt tcaggagctg
     1021 cttttttgat tcagtctttt ataaatagaa aatagggtta ataataggaa tgagaagcac
     1081 gtcgaaagat cttaatattg ataatcggct gggcatacat gatttccacg tcatcaatca
     1141 aactacctaa cagatgataa tcttcccgat catgtcgggg attacctaat agttcttgat
     1201 agtagatttc catactaggg tcccgaggat tgttcagcaa atcagcaagt acatctaaat
     1261 cttctggctg agtttctgcc gtaccctgta tctctatgaa aagcccttcc tcagcaaatt
     1321 ctactccaat acgtatttca gttatttcat gggaatataa ataagtaaga agctcatcga
     1381 tgattctttt tgataattct ttttccaagt gtcttttttt cctttctgac agcggtaaag
     1441 ttatctagta tcggtacaag gatacctgca acgaaaccag ttgaaaaacc actgttatat
     1501 agatttagtc ctccgtgcaa agtgctgacg ttatggacaa gagcaatatg tagtacacca
     1561 gctattacac cgtaaaaact gccgtaaaaa ccgcttatgg gcgcgagacc tgtcccaaac
     1621 acagctgcaa caagcatgcc agtactggtg atctcatgaa aagttccaaa caaagaagca
     1681 ataaatattc cgactagtat cggaaaactg ttttttaaat gacaaccaaa agcactgaaa
     1741 cccaccgcag ataaaatagc accgatgaca ggtccattta actgaccgcc aaccagcaaa
     1801 acataagaag acagcatcag accaactaag cctatgttga tcaaagtagc accaatacca
     1861 gaattagcaa tgaaatcagt tgtcaatttt ccagatgaat cgaaaatttc tcgaatcccc
     1921 aaaagccgaa aggaattata atagaaacca attccgactg tcagcgaaaa aaagccaaaa
     1981 ataatccaaa tgagaggaaa gtgatattca ttgaaaacca atgtattttc gacgattgaa
     2041 tagctgaata acctcaaaac agcagtaaaa gtcatcgcaa tcaacccaga ggtgaatcca
     2101 atattataaa gtgtgaatcc acgatggaaa actaaagttt gagcagctaa gggcggaaga
     2161 agtagaccaa ttgctatgcc agctaaattc cccaataaaa tacctatcag taaaggaaaa
     2221 cgcataccaa aagtgatata actaacgacc ggtgataatg cgctaccaaa cagtccgatc
     2281 atgatgtact gagaaaaagg tttattgaca aatacagcgt atagatacac acctaaaatg
     2341 attgagatag aattataaag gtttttgcca aaaaaagaga aaccggtaac tgttagaatc
     2401 gaagcaaata agggaccgtt taaaatcacc ttgtgccgat aagctaaaag cacagaaagt
     2461 aaggtcaaaa tccctacatt tatgaatgcg cttccgaaac cgcctaaagc aaaataatct
     2521 gtcaaaagat tgcttggcga ggtaagaata gttagcaatc cactccaatt atcagtagga
     2581 gtattagaaa gcaagccaat aaaaagaaaa aaaacacctg taaaaccaag ataaaaatac
     2641 tttcttttct gtgaaacgat tcgtttttga ggatgaatat aaataacgtc agtttcgttt
     2701 tccattacac accttctttt tcattttctt taactataac ttgaaatgaa agatttggac
     2761 aactatcatt taggtcaaaa ctaaaaatgt aagggaaccc attgaattta accaaatttt
     2821 tttaattttg caaaaaaaag ttaaagaaga ctgaaaaaaa atttaaaata taacgttttg
     2881 agtaaaatgt ttgcaaaaaa tgctaaaaat agggtacaat aaaacaacat atactgcagt
     2941 aaagaagtgg gggagtttta atgagaagaa aagtctatac caaggatcaa attctaaaag
     3001 ctgcttatga tgttgttgca aaggaagggt ttaaaggatt tactgcgaga aatattgcta
     3061 aaaagatggg gatttcgaca cagccgattt atctggaatt caaaaacatg gaagatttaa
     3121 aaaatacgtt gctggattct atctttgaac aactaaaaga agatgttttc cctattgaac
     3181 gtactggtga taaaatcatt gatctcggtt tgaactatat ctactttgca aaaaagcatc
     3241 atagtcttta catggcattg tacattgatg aacatgggga cggaaagaaa atgcatgatt
     3301 tttcatacaa ttatttccgt gaaatagtta aagaagatga aagatacaat caattatcgg
     3361 atgaatatgt cgatgctctg catactggta catgggtagc agtgactggc ttagcgtcat
     3421 tgatgtcatc aaacatcatg catccaacag aagaacaaat cattgcactg atcaaacgga
     3481 caattgactc gattttagca ttagataatc ctgaaataat cagatagtgt tctatcagta
     3541 tgaatttgat cggtaagtcc gttggctgca aactgtcaat ggactttttt gctaaaaaaa
     3601 gcgatttctg ttatttatct attagaaata ggcttgacat ttcgtgaaaa aagtttaaaa
     3661 atagtactta cggaaggttg gcagagtggt aatgcaccgg actcgaaatc cggcgaaccg
     3721 gctaataccg gcgcgcaggt tcaaatcctg taccttcctt atatatcaac gattagacac
     3781 gatttcgatc gtgtcttttt tgttgtatat ttagcctaaa atgttttttg actccccttt
     3841 tgactccccc tgagtactgt tcttcataaa tgctacaaaa ttattgatga cttttttgtt
     3901 ttgagtttca gttacatgag tgtaaatatt tgaagtagtt tgaatgtctg catgccctaa
     3961 acgttcctga acatctttta aagaagctcc tgattcaaaa aggagagagg catgagtatg
     4021 tcgaaaacca tgagtagtga ttcttttagt caattctgga tgatgcttat atatgacatt
     4081 aagccacgaa cgtgaaactg tagggttata gagaagacca tcatggtgaa aaattaagtt
     4141 ttccctttgg attgtaacga tcgttttgtt ttttgtataa tactttctca ataaactgat
     4201 caaatcattg tccagataaa tttttctttt tccagatttt gatttaggag tatttacgat
     4261 caatcttcct tgataacctc ttgttacagt tttattgata ttgagtgttt tgctcttaaa
     4321 gtcaatatcc gaccaggtca gagcaaaagc ttctcctttt cttatacctg taaaagccaa
     4381 tagggaaaag aataaatatc tttcatcatc atcgtttctt attgcttcta aaaatttgat
     4441 taattcttct ttagtataaa attcgatatt tttatcttcg atatctagag cttctccacg
     4501 tggaatagaa actaatttca taggattgct agttataatt tgtaaagaag cagcgtaatc
     4561 aaaaacatta gatgtgtaat tttttatttt cttaaaaatt ttaggatggc tatcggacca
     4621 agtgtttact gcttcttgac agaaaaaaac gtctattttg tcaatgaact tatctccaaa
     4681 cacttttaag atgtgtgtgt caaaaatttg tttggtggag gaccaagtgc tttctttaac
     4741 tgtcttttta taattttcaa accataagcc gtaaacatat tcgaatttct tgctcttttg
     4801 agctgtagaa gcttgcagtc ctttttcctg tatatctgcc tccaatcttt ttaaagctct
     4861 ctcagcggct aatggcgtac taaaacctcg tctcgttgtt tttctttttt ttcctgttag
     4921 aggatcaact cctaagtaca gactaaactg atatttttct tcgccttttt ttgttaagtg
     4981 ctttttaatt cgtttatcaa tttctttttt tgccatcttt atctttcctt tcgtacgttt
     5041 gttcggttgt acagcggatt tcgagatggt aaaatagggt acaacaaata gacctacttt
     5101 accgtagctc tttgcacatt tgcgttcttg gtcgggcggc aaatgtgctt tttttatttc
     5161 actcttagtt cttgtcctgg ataaagcatg tagttgtttg gatccatacc gtttaatgca
     5221 aacagttgat ctaccgtaat tccagctcgt tcagctattt gtttggtccc ttcaccaggt
     5281 tgcaaagtca aggtttcggt ttcagatgaa gaaggtgccg ttggttgagc ttgattagct
     5341 ggtacataat cttgtggtac tgattgctcc gttgaactgg gagtgggtga aacttgttca
     5401 acacttgaac taggagtagc ttctgcatag ttttctacac tttcagcaga gtaagtacta
     5461 ctagtttcag gaggcgtatt ctgcgcaata gcagcttgtt ctacagtact agaagatgca
     5521 atagtacttt ctgttgtagt cgaagatgat tgttcgttgc tacttgttga gctagtagtt
     5581 gttctagatt ctttagtttt tttccttttt gtagggtttc aaaactaatt tctcattatc
     5641 atcagatttg ttaggctttt ctggagttaa aataatattt ttatcttctt tgctgacagt
     5701 atatttggca tcattatctt tgtctttatc atctttccaa gtcataacat cgccttcaag
     5761 tgtatactca aatttgtaat tcatttggtc gactaagctt ttagcaaatt cttctcccat
     5821 agcctcccaa tcgttacttg aagttgattt catagaactt gtgtcaacac taaaagatac
     5881 gacgtgatca gaaaatgata cgatcatatt aggttcgtca tctttggttg cttcaatcaa
     5941 ccaatcatta gcttttaaat cttctgtagt tactttttta ccacaagcag tgaaaagtaa
     6001 cagagaagca aaaactaata aacttgttat tacttttttc attttttatt cctcatttct
     6061 atgatatgat ttttattggg gaatcttaga aataaggttt cgagtccgtg ttgctgcacg
     6121 ggcttttttt atttaaataa atcccaaaaa ctgaatgtag ttttcttgta tactttattg
     6181 taagcagcct tttttgggtc tttaatccag ccgctacctt ttttcccata accaggaata
     6241 actgcttttt ttacttttct ttttgctttt ccagtggttc tagcgcttat ggattttttt
     6301 atacttggtt ttctcattcc tattttcatc tatatcctcc ttacgttgta ttataattca
     6361 caagaaacga tcgaattcat ctggtaatcc aaagcagacg agcttttcat attttgtcat
     6421 atgatcaaag ccgtctaaag ttggatcaat agtcagatga gttgcgaaat aatttgcttc
     6481 tttttcaatt tttaattcag atactagcgt ttttttagac aacataggtg tgttttcatt
     6541 cggatgcaaa acacagtggc ctaattcatg actacaagtt atccattttc ccatctcatc
     6601 taaactagaa tttatgtgga tcatttttat ctgtttgaat ttactatagt aaccataaat
     6661 ttctcccaaa tcttcttcta aaactagtat cttcaattct cgtgcaattc taaaaggatt
     6721 gcgtgtttga tatagtttta ccagcttatt aattttgtta tcaatttgag gtaaatacat
     6781 aaacaacgta ctccttttaa tcgcgatatt ttttaggtgt gtattttttc ttagcttcta
     6841 ttttagctat tcgcaaagca ttctctaaag aaataatgag agcttcacgt gtcgcttcac
     6901 tcatttcatt atctttcttc gaaaaagcca ttccatcagc attagataaa tcgtcgatta
     6961 atgactgcaa ttctttttgg atatcacgct cgtctttttc tgttaaatca tagtatcttt
     7021 ttttctcagt acgtcctagc aaataatcta cagatacgtc gaagtagtca gcaatttctt
     7081 gtaacttttc ggcagttggc tgttttcctg cgtttaaact ataaaagtaa ttctctccgt
     7141 acttcaaatc tttagcgact tgttgaaggt ttttattctt gtttttcgct agttctttta
     7201 ttctttcaaa tgttgtcatt ccaacgttcc tttccaacac gaaaaaataa tttacgaaaa
     7261 agagtaaaaa tagctttaca attttacgat atagagttat tatttatccg taagttaatt
     7321 tgatagaaaa aagcaaacta aaaacacacc ttatagcata aagtttggcg accaagtgca
     7381 aataaaaggt tttgttatag gcttatttct ctatgtattc attttactct atagtgtaaa
     7441 aatgtcaatg gaaattatat tttttctatc attttaactt acaaatttat aaaaaaagga
     7501 agtgggttaa tgtcaaactt ggataacggt cgtgcggcga tcaaagagtt tatgaaagag
     7561 aatggaataa ctatggatga tttggctact gcttatggtc acacgcgtgt acgaatgcaa
     7621 caaattcttg atggacactg gagtggacca aaggcaaaca gaactattct agagatcatc
     7681 cgtgattaca gaattcgata ggaggcaagt catgaatatt ctaagcgaag agtttttgac
     7741 tcgtttaaga attgcaattg ttgaagttgt aaaggacgca cttagtcaac tttcaaaaaa
     7801 gaatttgtca gaaacacgat atttaaaaaa gatcgaagct agaaaatatg tgggaggtgt
     7861 aaacgatcaa ggttttgaga agttaatagc tcacggttta aaagaaattc gtatagatgg
     7921 ctttttaaga tatgacaaaa aggacatcga tgaactgatg gctaaataca aaatttaaag
     7981 gaggtcaaca tgggaaagtt taatcgaaca ttagtattca gcgcgccact aattatctac
     8041 gctttaggtc tatggggtag tagacaagca ttaatcggca cgatcgttta catgctttgg
     8101 attttcattg ggcttgatga agctgagtac agagcgaaaa agccagtcgg gagggactga
     8161 ctaaaaaagt ctaatgtcta taagaaaggt ttgacaataa atgaaagaat tatttttttt
     8221 aataactttc atcatttttt ctatcagtgt tttctacatt aacctttgcg ttgctagatt
     8281 tgctttgaaa agttttagga acagcaaacg aaaatagagc tataaaagat acgaatgtag
     8341 tagctacgtt aaattgctct ttagaaaacg tcaatgtcat tggaatgata attgatatcg
     8401 atgagaaaat ctttgcgata ataactatcc atttttcaat tataaacatt tttaaattaa
     8461 gcaccattag acaaaatgta gttcctatat agaggataaa tgaagataga aataaacata
     8521 ttagtccagt tttaaaaaaa gagaagtaat ctatgtgggc aaattctgtt aatatgcttt
     8581 tttgatttaa cacaatcaaa ctactgttgg acaaaaatga cagtaaggaa agagttgctc
     8641 cgattactaa ataaaaaata gttgttaaca atattatatt atcaattttt ctgctcatgt
     8701 tttcacccca ccagaattat atcaaaaagg agagaagaaa taatgcaaga attagtaatt
     8761 ttgaaaaata aagaagctgt gactacgagc ttacaagttg cagaaagctt cgataaaaag
     8821 cacagacatg ttttgacggc tattgatgaa ctaaaagagg gggttgccga aaattgggca
     8881 gacctatttt gggaagatac ttatgttcat cctcaaaata aacaatcata ccgaattatt
     8941 tatatgaata gagacggctt ctctctatta gcaatgggct tcactggtaa gaaagcttta
     9001 agttttaaac ttcaatatat tgaggccttc aacaagatgg aaaaagaatt aaaagaccaa
     9061 ttgccatcaa ttccagcaac taaacgagaa atggcattac ttgctctagc tgcaaacgaa
     9121 gaaacgaatg aaagagtaga cgctatcgag tctgatttaa acgatttgaa gaacaatcaa
     9181 cttctagctg aaccagatta ccgaacaatt tctaatatgg ttcgcaacaa aattagagtt
     9241 atttgtaatc agcagcattt aaatagcaaa gcaaaagcag aattgtttaa agacttgaat
     9301 ggcggaatta agcgaatcac tggagcagta gctagaaatc gtatcaaggc aaaacagttc
     9361 gatgatgtta tcgattttat taacaattgg atgccttcta cagcgacgat gacaattatt
     9421 aagcaaatgg agttgttaga ggatgaatag agctgaagcg ctaaaaatag gcgtaataat
     9481 tgctaatcgc tggtggagac acaataaacc aaacatccta agccaacaac atattgataa
     9541 gcaaaaagca tggcaacaaa taaaaaagtg actcagccga ccaaagcaat gagtcacaaa
     9601 gaaaatacat ctaaggagat gttaccacat gaaaaaagaa ctttccactc tagatcaata
     9661 tttgactgat cctagttggg gcaaatcgaa tatcaaggaa acaagcaatc gaaaaatcag
     9721 acgtaatctt ttgacggatg aagaactagc atgtgatcaa gacgatttgg gaaattttgt
     9781 gagtatttgg gatcatgtct atcttatcca tttatcgagg aagtccaaaa aaactgaata
     9841 tatctatgtc atcgaagatg gcttgattga tgcgctagaa gagtatgaca gagataactt
     9901 gattgatatc tcttattacg gatcaggtaa gaaatacatt gctgaaatgg aggcagaatt
     9961 tgatgagtga aaaggaacaa cctttgaaaa atagaagtga taatacgctt tttaatacct
    10021 tatacaaaat aaatgtgaaa gatgttactg aaaaacgaaa taacctcact tatctttcgt
    10081 gggcatgggc ttgggcagaa gttagcaaag tgtgtgaggc agtagattat gaaatctatc
    10141 atgatccaga aacatatctg ccgtatgtct ttgataagaa aacaggctat atggttttta
    10201 ccagtatcac agtcaacgga gtaaagcgtg acatgtggtt accagtcatg gatggtgcaa
    10261 acaaggcaat gaaagatgag ccatatacct acgaagtcaa tgattatcag tggaataacg
    10321 aaacgaagaa aaaagagatt gttggaaaaa tcgaaaagcg agttgaagca gcaactatgt
    10381 ttgatatcaa caaaacgatc atgcgctgtc ttgtaaaaaa tctagcaatg tttgggctag
    10441 ggctatatat atttgctggc gaagatatgc cagaagatgt ctcgatgctt gaaccagcta
    10501 ctcaaagaag caaaaagcta ttcttggatg ctttacaact ggttgctaac aagtacgaaa
    10561 aatcaattga tgaagcaatt gttgcattga ctgatgcggc ttctataacc gctgatgaca
    10621 gtaaatggac caagagagac ttgggcattc taaaacgagg cgttaactgg attgaagatc
    10681 agtacagaga agaaacaaaa gagaagtgat atgagtgttt aaaccattga tcgattcata
    10741 ttcagcggtt ctgaaaaagt tcaaaggaaa agacataggt gcaacgatca atgaagaagt
    10801 gaacattgat cgactaaaga cgatgtatga cggatatgat ggcgatcgga ttattgaagt
    10861 tcgatttatt gatccacgtc gatttactgt gcagcaacga aacttcatct atgcgctgat
    10921 aggcgatatt ttcatcgata caggcatgcc aacggacttc tggaaggaat tcttttactt
    10981 ccgttttgaa ggcgtcacag ggcgcaaaat aagcctcaaa gacgaatcga atacaactgt
    11041 gagtgatgct aatgtcttag caaatatcat cttagatttc atctttgaac atcatattcc
    11101 tttcaaagaa ggctatgaga ttttaccagc gaatcaagaa tattacttct acaaatgcat
    11161 cacaaaaaga gtctgctgca tctgtggcaa aacaggagct gacatcgatc actttgacaa
    11221 agcgctagga agacgaaagc gcaaagaagt tgatcattca gagtacacat ttgcagcact
    11281 ctgcaggatt catcacacgg agaaacacaa tataggtgtg atcaatttca aaaataaata
    11341 tcaaatcaaa gggatcaagt taaaccagga aacaattaag aaacttagaa taggagggta
    11401 aaaaatattg tctgacaaca aacgctacta ctatttaaaa ctaaaagaga attttttcga
    11461 tagtgacgag atggttctct tagaaagtat gccagatggc tatatttatt ctaatattct
    11521 tctcaaactt tatttaagaa gtctaaaaca cgaaggtaag ttgatgttta atgacaggat
    11581 tccatttaac tctacaatgc tcgcaactat tacaagacat tctgtaggag tcgtagaaaa
    11641 agcggtacaa attttccgtg atttgcagct tattgacgta ttagataacg gagcaattta
    11701 tatgtctgat atacaaagtt tcattggaaa atcctcaact gaagcagata gaaaaagaga
    11761 atacagaaag aaaatagaag aggcaaaacg gaatttaata actggaggac aagtgtcgga
    11821 caaatgtcca gacaaaacta caccagagtt agagatagag atagagaaag atatagatat
    11881 agataaagaa gaaaagaaag gtaagtattc tgacgaacac ttacgccttg ctaaaaaatt
    11941 gcaaagtaat ttaactgaag attttccaaa agaaatgaac aaagtagata tcgaaaaatg
    12001 ggcagacaca atcaggttga tggaagaaag agataaagcg tctatagaag cgattgagta
    12061 tgtgatcaat tggctaccta caaatgaatt ttggtttgga aatattagaa gtgctaagaa
    12121 attgagagaa aaatttgaga agctcaaatt cgaaatcaaa gcagacaaga ataatcataa
    12181 aaagcaaagt caaaaactac agtacagcaa tcctagtgaa tatgacgact tgccaattta
    12241 aaaaggagat gcatcacatg gaaagcctag caaatgctat ggagaaacta ataagaagag
    12301 tattagtgca aagcgggaaa tgtccagaat gtagcgaacc tttgtatagt tggcgagcta
    12361 aaaataagga tggttcagaa cgttgtaaac caacatgcat gagttgtggt tataaagcgt
    12421 tacgtgtgaa agaggatata cagaccgaac ggatatataa cgacagctta aaagcacgag
    12481 cgttgagttt ttttcaaaat ggttcggtat taacagataa aactttgttt aaatgcaaaa
    12541 tggagaatta tcacgtagtg gaccaagaaa cgaaaattgc tttagaaaga gctaaaagct
    12601 ttgtaaatga tgtcttactg aaccatcctg cacatttcat tctatcaggg aaatcaggaa
    12661 gcggaaaaag ccacttgtca atggcgacag cttgggaaat acttgagcgc tcaaattatg
    12721 acaagaaaat actttttata agctatcaag agttattaga gcaaataaag ttttcttata
    12781 acaatgctga actgagaaaa gaaattgaag gatcgcttat agccgatatc aaaacaactg
    12841 atttggtggt ttttgacgat attggagctg aattaggtag cggggtatca aatagtaggc
    12901 agtttacaaa caacacgtta aacacgctct tagaagccag acagaacaag gcaacgatca
    12961 tcacaacaaa cttatccggt cctgaactaa gagaagccta cggcgaaaga attgtttcta
    13021 gaatatttaa gaattcagaa ggttatgcgc tgaaattcca acaaacagca gacaagcgca
    13081 taaaaccagt gaaaggtagt atcgcatgaa taaataccgt aataagaaaa ctgttcatcg
    13141 aggtatcaag tttgattcta tcgcggaagc agagtattac gatttagcct tgtggcaagc
    13201 agaagcgaac ggctggaaag taaaacttca ggaaagattt gagctgatgc cgaaatttga
    13261 actagatgga aaaaagtatc gcaagatcga gtatattccc gacttcacat tttataaaaa
    13321 cggcaaactt gtcaaagtcg tagatgtcaa aggaatgcag acaaaagact ttaagatcaa
    13381 ggcaaagttg ttctgtcatc aatatcaagt gccgttgata ttagctaaaa aatatcggaa
    13441 tacgttcaag gaagagcgtt tttaacgagg tggtccatca tgacaacaga agaagtgatt
    13501 caaatgcgta ttcgaagcct tcaacgtgag attgacgatc tggaacggat aaaggcagtg
    13561 atggtcaatg aaacggcgag aaaggcaatc aatttgcaca tagtgaattt aagaagggaa
    13621 attcgtagat tggaggaatg agcgtgggta agaaagcagc aatgaaacga atcattgaac
    13681 tgacacattc tgagaattgg caagaagaca aagaaatagt tgcagaagtc caaaagctcg
    13741 gtaaatcaat gtggactgaa aagcctaaac ggaaaacgcc gagaaaaatt gcaatctggc
    13801 atggtgatcg aattctagta acgggtacag ctgaacagtt atctgaaatt actggactga
    13861 gcaaaaacat catctgggat agagctagga gcttatggat tgattcaaag gggcgacagt
    13921 ttaggtatgt ggaggagaaa taatggatct cattacacaa tacagtgata tcatcctcaa
    13981 gaaaatcatg atgaagattc agaaagataa aaaagcaaaa gaacgagcgg aattagttaa
    14041 gttggaaatg gctgaaacag gagcaggagt gcgaagtagc aggcattgga aagcagcagc
    14101 aaacattgaa ttttattaca acgaaattca aaaagggttc gatcagatgc gtgagctgga
    14161 tcggcaaaca aattggagca agaaacttca tcaggatcgt ttcaaatttg tagaaaagta
    14221 tagagagata ctgaatgagt atttcgagga ggactagatg tttaattcaa cgttagcgtc
    14281 aattcacttt ttattctata taacgatatt tgtagctata ttaattaatg cgataaaaag
    14341 cggtttttta acaccaatcg gcacaatttt aacattagct tcatttgtta caagttgtat
    14401 tatccaactt aaatatataa aggagaatga ataatggacg aactaatcac aaaagtagag
    14461 cagtgggcta aagataaggg attggatcaa gctgat
//
LOCUS       DACCYH010000070        14481 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.073, whole genome shotgun sequence.
ACCESSION   DACCYH010000070 DACCYH010000000
VERSION     DACCYH010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.073"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            621..1484
                     /locus_tag="IVY85_002066"
     CDS             621..1484
                     /locus_tag="IVY85_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8045076.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1613..3235)
                     /locus_tag="IVY85_002067"
     CDS             complement(1613..3235)
                     /locus_tag="IVY85_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8045077.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3484..4005
                     /locus_tag="IVY85_002068"
     CDS             3484..4005
                     /locus_tag="IVY85_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045078.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4191..5921)
                     /gene="spxB"
                     /locus_tag="IVY85_002069"
     CDS             complement(4191..5921)
                     /gene="spxB"
                     /locus_tag="IVY85_002069"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP8045079.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            6086..6904
                     /locus_tag="IVY85_002070"
     CDS             6086..6904
                     /locus_tag="IVY85_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP8045080.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(7007..7204)
                     /locus_tag="IVY85_002071"
     CDS             complement(7007..7204)
                     /locus_tag="IVY85_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045081.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7373..7951
                     /locus_tag="IVY85_002072"
     CDS             7373..7951
                     /locus_tag="IVY85_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8045082.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8996..9499)
                     /locus_tag="IVY85_002073"
     CDS             complement(8996..9499)
                     /locus_tag="IVY85_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8045083.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9858..10883
                     /locus_tag="IVY85_002074"
     CDS             9858..10883
                     /locus_tag="IVY85_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8045084.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11206..12084
                     /locus_tag="IVY85_002075"
                     /pseudo
     CDS             11206..12084
                     /locus_tag="IVY85_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12560..12676
                     /locus_tag="IVY85_002076"
     CDS             12560..12676
                     /locus_tag="IVY85_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8045085.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13604..13942
                     /locus_tag="IVY85_002077"
     CDS             13604..13942
                     /locus_tag="IVY85_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045086.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            14036..14266
                     /locus_tag="IVY85_002078"
     CDS             14036..14266
                     /locus_tag="IVY85_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045087.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         4587 a         2425 c         2760 g         4709 t
ORIGIN      
        1 ccgtgtcaca gtcccttttt ccgaacaaac ggtgttcaaa agcttgcatc tacggcacta
       61 agcccaatca gatgaaaaat atggagtgct atttttatct gattgttctt actgcttgat
      121 gcagagcagc tttttcacaa cctctccact aaacggtgtt ctcctgccaa ctcctcggtc
      181 acaagtcaca atattcaaca aacatgagaa gctgtttttt gaaattgttc cttgtgactc
      241 ggagctaaac ggcaggctca caacctcttg gtaaacggtg ttcaatcagc tgaccctcgg
      301 tattagctca aaaaacggca aaatatgagg aactattttt tccgtttttt tctctaatac
      361 tcaggtcaaa acgctgattt cacaacctct gattcacggt gttccagaag tagcttctac
      421 ggaaataagc cgaaatttca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt
      481 tcttgaagct gaccacttct gtcacaacct cttattttga ttagaacttg cgaaaataaa
      541 ccggttaaag tgtgtctagc gcaagagatt tgctataata gacgataagg gtaaaatgta
      601 acgagaaggg gttgaaaaat gtgtatcaaa ttgtaacaga ttcttgttgt gatctgccat
      661 accatgtatt agcaaaagaa aaagttgatt ttttatcaat gaatctccaa ttgaatgatc
      721 aagagttagt agatgacctc ggaaagacat tcaattatga ccgctacatg aatcagctga
      781 aaaatggtgc gatgcctacg acatcacaaa tcaatgttgg ccgatattta gaatttttcc
      841 gttcttatgc ggaaaaacag attcctgttt tatatgtggc tttttccagt ggattaagcg
      901 gctcttacag tagtgcgctt caagcggttg aattgctgaa ggaagaatat gaagaccctg
      961 agatctatgt ttttgataca aaagctgcaa gtattgggga agggctttta gtattggaag
     1021 ctgtaagatt gaaaaaagaa ggtaaaagca tcgaagaaac cctagagtgg ctggaaagta
     1081 attatttgaa agtccattca tgggtaacag tagacgatct gaaatatcta gaacgaggcg
     1141 gtaggatttc caaagcaagt gctactcttg gcagtctatt gagtgtaaag cctattatta
     1201 cggtggataa agaaggaagg ctgacgaatg ttgacaaagt tcgaggaaga aacaagtcga
     1261 tccagacaat cgtcaatgag acggtaaaaa caatcgttgc accacttgat caaatcgttt
     1321 atgtggctta cgctggggat ctagagagcg cggagaaggc aaaacggctt ttggaagagc
     1381 agataaagat gaaagatgtg aagctttatc ccttaggacc aacgatcgcc agtcacacag
     1441 gctatggctg tatcgcgatt ttttcgatgg gaatcagtcg ataaaagcag taactcattc
     1501 tgtaaaaaag cccagaaatt gattgtaaca aaaacagcga ctcttaaaag atagatgagc
     1561 atcctatctc ttagaggtcg ctgttttttt attttttaag ctaaataggc cattatgccc
     1621 ataactcgtt caattgtttt tgaatatctg cattttccaa aaattcatca tatgtcgtat
     1681 cagctcgatc gatcacacct ttatccgaaa cggcaatgat gcggtttgcc aatgtttgga
     1741 taaattgatg gtcatgtgac gcaaataaga ttgagccagt aaatgccatc aagccgtcat
     1801 tcaatgctgt gattgattct aagtctaaat ggttcgttgg atcatctaat acaagtacgt
     1861 tagctttcga aagcatcatt tttgaaagca tgcaacggac tttctctcca ccagataaaa
     1921 cattcactgg tttcaatact tcttcaccag aaaacaacat tcttcctaag aagcttctta
     1981 agaaggtgtt atcatcttct tctttacttg catattgacg caaccagtct aaaatcgtta
     2041 gatcagtatc gaattctttc gttgtatcct ttggtaaata agaattactt gttgtcacac
     2101 cccaacgtac tgttccagta tctggcgtaa tttcacccat gataactttg aatagcgttg
     2161 ttgttgttat atcattttct gcgacgaaag cgaccttgtc ttctttgctc aaattaaaag
     2221 atatatcatc taaaattttc tttccatcaa tcgttacact tacattttct acctgcaata
     2281 agtcgtttcc aatctcacgt tcaggtttga atccaacgaa tggataacgt ctagaagaag
     2341 gttggatatc gtctaaagta attttttcaa gcattttttt acgtgaagtt gcttgttttg
     2401 attttgatgc attcgcacta aagcgggcaa taaattcttg gagttctttg atttgttctt
     2461 cttttttggc atttgcgttt gcttgaagtt tagccgctag ttgactagat tccaaccaaa
     2521 aatcataatt tcctacataa agtttgattt taccaaaatc tagatccgcc atatgtgtac
     2581 atactttgtt caaaaagtga cggtcatggg atacaacgat cacagtattt tcaaaattga
     2641 tcaaaaattc ttccagccaa ttgatcgatt ggatatccag accgtttgtt ggttcgtcta
     2701 gcaaaagaac atcaggttta ccaaatagtg tttgtgctaa taacactttt accttttgtc
     2761 caccagttaa ttcactcatt tgctgatggt gcaattcttc agggatgttc agaccttgca
     2821 gtaaaacagc tgcttctggt tcagcttccc atccattaag ctcagcaaat tctccttcta
     2881 attcagctgc tcgaatacca tcttcgtcag tgaaatcctc tttcatgtaa atcgcatctt
     2941 tttctttcag cacttcatac agacgcttgt gtcccatgat gactgtttca agtacagtat
     3001 agtcttcata gtcgtaatgg ttctgtttca atacggccat ccgttcatct ggtcccatag
     3061 taacggaacc tgtggaaggc tgcagatccc ctgataacac tttaaggaac gttgatttac
     3121 ctgcaccatt tgcaccaatc aagccatagc agtttccggg ggtaaattta atattcacat
     3181 catcaaatag tttgcggtct gaaaagtgca aactcacatc atttacagta atcaattttt
     3241 ttcctcactt ttatcattat tagttttgac aagtcttata gaattatata ggggaaaggt
     3301 aaaagattca aggcaatcga acattctttg tgtttcacgt gaaacagaat gaaaaaaata
     3361 aaaataaaag cggagcgaaa atagctacta taggatttat tagtttgcaa cagcataatt
     3421 tttgacgtta tttataagag ggagtactat aaaactgtaa aagaaatgaa agaaggattg
     3481 aaaatggatg aaaaaagaac catcgactgg ggctcattga ttttgggaat tttatttgta
     3541 cttgtctcat tgttatcatt tcaagaccct gtcggaaatt tagttgcaat tgtagtagtc
     3601 tttgcgattt ttgcctttat taaagggata tttgaactgt ttgtacggaa cagattaaaa
     3661 gagctaacag gatataaagg aaagatgccc ttaataatcg ggatcatcga tattctagtt
     3721 ggcgtctttt tcttattcaa tattggtgca ggagtcgcag cattgccttt tgtttttgca
     3781 gtatggttta ttgctgattc agttttagcg ttatttactg ctgacttggc aagaggagta
     3841 agtgaaggat attattggtt tacgatcatc gtcaatattt taggaatttt attaggaatt
     3901 ttcttattgt ttaatccaat ttcttctgcg ttgacattaa gctttttagt tggattctat
     3961 tttatgttat ttggtatcac acatatcgtc tatgcattta gataagtaaa atcaaacaaa
     4021 taaaaaagcc ggcttccgat tacagtttta ctgttgtaat tggaagccgg cttttttatc
     4081 atggacaaat atgcacgagg tgatttttag gtgtcgagac aaatttatgc aaaaatgtga
     4141 gaagctatat acacaaattt tatcttaata ctcgaagcat agcgcagttg tcacaacctc
     4201 ttttcatata attcattcaa tactggcaaa tcatggactt catatttttc tttgaatgct
     4261 tggatttctt cttcagaata ttttcttgga tctaaatgca gatcttctac tggcaatggg
     4321 cgcttgttat tgatcttcac atcgattact acaggacgat ctgattttgc agctgcatca
     4381 aaggcaaatc gaagttgcga gtattctgtg actgtaaagc tatttgcccc gagcgcttct
     4441 ccaactttac cgaaatctgc atcttctaaa aatacaccaa atttcttttg ttccgtatct
     4501 tcttgttctg cttcgatgaa accaaaagaa tcattcgaaa agacaacatt aataattgga
     4561 agctgatatt tgacttgtgt aatgatatct tgcatgacca tagcaaaccc gccatcacca
     4621 cttaaggtaa atacttgttt ttctgggtag cttagttgtg cggcaattcc tcctggcaca
     4681 ccattaccca tagttgcgaa ccatccagat gtcgtatgtt tttgtttacc gttcatattc
     4741 aataaacgaa tagaatggat agtcgtgtta ccgacatctg tgacgaaaat cgcatcgtct
     4801 tcagcaatac gattgatttc tttatagaca gcctctggac gaatcggttc ttcctctcga
     4861 tcttcaaaag atttcaacca atttacccat ttttctttat tttcttgatt tgctaggtac
     4921 cagctatcag cagggcgagc atcccctaac tcgacaagtc tttgcaaagc tgtgtttgca
     4981 tcaccgagga tcgacaaaga tgtttcatgg cgtcgtccaa attttgacgc atcgatatcg
     5041 atttggataa attcagcagt tggattaaaa aatgcacgtc caaatgggaa gtcgctaccg
     5101 acaaataaga tcagatcggc ttctgccaat gcttcatttc cgggtttagt agcgacacgt
     5161 ccagcaaaac caagaaagtt ttcataatca tcaggaataa ttccttttgc tagaacggaa
     5221 gcgacaacag gaactgaaaa atgttctgag aattctttga tttgcggaaa tccgtttcgc
     5281 gttccttgac caacataaag aactggtttt tttgcttttt caagataagg caaagcagct
     5341 agcaaatctt tttcttctgg taatatcaca cttgttttgt gagtgtgagc ggtggagaaa
     5401 ggttgttcgt caatttcttc aaaaccaaga tcaacaggga tagtcacgat agccacgcct
     5461 ttatgctcat aggcggcttt aatggcttca tctactacat gcggaagact ttgtggtgtc
     5521 atgactgtac gattgtatac gctaacatct gcgaaaatag gattttcatt tagttcctgg
     5581 aaagagttat agttcatgga tgtagaagca acttgtccta agaacgctac tagaggcaca
     5641 tggtccatct gtgcatcata caaaccattg atcaaatgtg tcgcaccagg tcctgcagag
     5701 ccgaagacag cgccgacttt ccccgttaat tttgcatctg cagcggctgc gagtgcgccg
     5761 acttcttcat gacgaacttg aatgtagttg atttcggttt tttcttgata tagcgcatcc
     5821 ataattgaat tgaaagatcc tccgggaatg ccgtaaatat ggtcgatacc ccagctttca
     5881 agaactttta ccattgcgat tcctgcattg atttttgaca tggtgtttca ctcctttagc
     5941 aatgatgata cataaatttt ttgtatctag agtatagaac tttttatcga aaatcccaac
     6001 taattcgtct tttcacaaaa atgtagtcat gtttttggga atatgctaga gtagtacata
     6061 gatagattca agagagggga atggcatggt tgaatggcta ttgagacttg aaccttggca
     6121 gcaagcactt gctgggacag cttttactta ttttatgaca gctcttggtg ccgggttggt
     6181 ttttttcttt aaagaaatca aaaaagaagt gctaaacttg atgttaggat ttgcttctgg
     6241 tgtcatgatt gctgcaagtt tttggtcttt gttagatccg gctattacaa aagcagaaga
     6301 aaatggagat attgcttggc tagttgtaag tattgggttt ggtttaggcg gtgtgttttt
     6361 atatatggca gataaaacac tgccccatat gcattttgga ccacaacatg aagcagaagg
     6421 attgcctacc catttaaaac ggacgatctt gcttgttttt tctattacat tgcataacat
     6481 tcctgaaggt ttagccgtgg gggtagcatt cggtgcagca gcaactgcgg ataatccgac
     6541 agccgctata ttagccgctg tttcggtggc gctaggtatt ggtatccaaa acttcccaga
     6601 aggagcagct gtatcaatcc cgttgcgaca agaaggtctg agtcgtaaaa aggcctttgt
     6661 ttacggacaa gcttctggaa tcgttgaacc aattgctgga gtgattggag cgctactagt
     6721 aacgaaagtg gagctgctgc ttccttatgc acttgctttt gccgcaggag caatgattta
     6781 tgtcgtggtg gaagaattga tccctgaagc acagcagaca ttgtccagca aacgtcattt
     6841 tgcagttttt ggcgtcatga gtggatttat cattatgatg attttggatg tggcattagg
     6901 ataaataaaa aaaatatata tgatatatca aatagggccg cgaacttata tcgcgaccct
     6961 atttttttag taagttatat cataatgaag tgaatggtat catacattaa taatagtttt
     7021 ttctatgtgt atttcctttg atccagcgaa caagccagat gataagtgcg attgggatta
     7081 gaactttgac cgcaaaccat aaaagcccgc ttaggacact gacaaaaaga ctgatgacga
     7141 agctagctgc aattagcact aaaagaacca tgaagatatt taagaacata tttttttgac
     7201 gcatacaaaa ccctcctctt gttcattctt tactatagaa aaagtataac agggattgtt
     7261 taaaaacacc tccaactgaa gactgatttt tacttcatct acttaaatga tcaaacaatt
     7321 gctttttctc tggtttatca tctaaaatag agcggaaaag gaggagaata tcatgcctaa
     7381 aaatcgtctt gaagcattta cagatgctgt aatcgcgatt atcatgacta ttttagtttt
     7441 agaacttcat gaacctaacg gagacacgtg ggaggcgctc gctgcattag gttaccgatt
     7501 ttttatctat attgtcagtt ttatcatttt agcgatttat tggaacaatc atcatcattt
     7561 atttcaagtt gtacataaga taaatggacg ggtcttatgg gcgaacagtt tctttgtttt
     7621 tgcgctttcg ctttttccgt ttgcaacttc atggatcagt gagtacatca atagcctggc
     7681 tccagaaatg acttatggtt ttgttacctt gaccgctaat attgcgtatt atatcttggc
     7741 aaaagaactt gtcaaagcaa ataaaggcca cgagaaagca ttgaaggcga tgttgaataa
     7801 ttacaagaaa tcgtatctat cgattggttt gaatgtgtta ggaattgtct tagggtattt
     7861 agtagcgcca gtagccgtgc tgatcgcaaa catcgtgatt ctagctgctt ggatcatccc
     7921 aagtcgtacc atcgaatcac aatatcggta aaatgatccg tgtttaaaat ttaagagaaa
     7981 aatatagaaa aaataaataa acgatcaaat atccgagtga tactagtatt caaagaggga
     8041 tgcagatgga tcaaaaaaga attcatgtag gactgattac tagttttgtc acgatgctta
     8101 tttatttgga tcaaggtcat ttgacttgga cagtagttgg tgtcgtagga tttttgacat
     8161 tattcacagc aataaaaaaa taaacatctt tatgtaatct gatgttctat ctatttaaga
     8221 aaagcatgtt tacagcagta tgattctgtt gtaaacgtgt ttttatttgt ggaaaattta
     8281 atttttagaa aagtgtatcg aaaaatagat agttagtttt tccacagtgg ataacttcgt
     8341 aagagataaa aataaaaaat tagtcaaaga agggcgtata gatttctttt gataggagat
     8401 atacaacaga aatgaataaa tattaacgtg ttttcaaaca gtataaaacg gtgtggtttt
     8461 tggaataaaa aaataacata tctcatttta cacttttgtt attttatact taaaaaataa
     8521 cgacacagaa aataccgtgt ctcagatttc tctactactt ctaaaaacta ctgttttttt
     8581 cctttgaaat gccttcaaaa agtaggatca ggcagaatat actggcagaa tcaaggagaa
     8641 gggagcctaa ataaggagaa aaaccataga aaatataaag taattactta taaaacaagc
     8701 tttttataga aaaataatat acctgagtag ggtataaatt aagacggaat tcctttctag
     8761 attgagatat tgactaggaa gaatgttaag attttaagcc tttaagctaa aaaaaaaccc
     8821 tttttgttaa ataaggggaa tatagcctgt cagtttcaaa ctgcaagtcg tcctgctgca
     8881 ataatatagt agattagaga tgaaaataga tttttttaaa ccaatgactg aataaaaaga
     8941 ccgtcataga tacacattgg aatgtgtcta ttacggtctt tttttacgaa cgattttatt
     9001 gaagacggac agttcgggtc aaaaataaaa aagctacgat cagaaataag aataaaatgt
     9061 atcccaaaat aatggctaga cggtctaact tatttgtcga tagatgtttg tttagaagtt
     9121 gataggccca taaacctatc agtcctcctg ttgtattagt tatcaaatca gtgatgtccg
     9181 ctacgccaat accgaatagg aattgaatca attctgccaa gaagcttaca aggaaaataa
     9241 tccctaattt tttagaaaaa gaaagtctct tgaaattgac attcaaaagc accccaaaag
     9301 gaaaaaagac aatcaagtta taagtggtct cacgtaatac cgtcgtattt tcaaaagaaa
     9361 aagtagaaaa agggacgaga ttgacattgc tgtaagaata agtgagaaat tgtggaagat
     9421 taaatgatag cttgaataaa atcaaccata atagaaagaa taaataaatc cctaataaaa
     9481 attttgatac ataccgcata gacatctcct ccatttaaaa agtataccat cgaaaaccgc
     9541 tgaaaaatca taaaaaagta tgaagtttaa ggaccaacgt agttatcgta cgaaaattag
     9601 aagctttttt ttgttgggaa tccggtttct ttaacaaagc taagatagcg agttgcttgg
     9661 aaaagttgct atcttttttc tgtcaaaacg actcggaatg ttcgtttttt gttctaattt
     9721 ctccaagttg tttacttttt gatactaagt ttatgaaaaa tcaagaaata gttgttatac
     9781 taggtaatgc tgacaaacgg gattggcatt tcatcttggt tcagcttttt ttccaattac
     9841 taggaaggag ggatagattg ttcaagactt attttcgttt caataaaata caagataatc
     9901 cttttcgtat catgaatctg atgctactta tctttattgt attatggtta ggttacataa
     9961 atcatgattt tttgatttca agtttttcat cattagggat ctttactttt ttctattatc
    10021 aaaacatacc gatgaaatat ttgctgaaac gtatgtttct gatcggttgc ggactgttgg
    10081 ttgctttcgt tttggggatc ttatcgactt atgttttatg gcttgaacca tttgccgtag
    10141 ctttggttgc tttttccagc agattcgtgt tgcgattatt ccatatttct aaaccaggtg
    10201 gcttgttttt tgcgatgctt tctgccatgg gaacgagcat gcagctgcca atcgctcaat
    10261 taccgatcgt ctcactttat ttttttatgg gtgtcgtttt tgccctgatt gcggcagtga
    10321 ttacaaaatt actggattca cggcctgagc agacaattga aaaagcaact ttaaaagaaa
    10381 gatttcacga agagccttta gtgatcattg attctgtttt ctacagcgct gccttatttc
    10441 tttccgttta tgtcagtcac ggcttgaatc tgcataatcc ttattggttg accttgtcat
    10501 gtgcatcgat tttgctagca gaaaacttgg atgcgatgaa gcatcgacaa gtccaatatc
    10561 tgattggatc gatgggtggg ttatgtgtgt cggcattctt gtcatttgtg ccgtttacgc
    10621 agttgcagac catttttctg atcacttttc tatatggaat cgcacaattt ctagttgcta
    10681 gaaattatgc agttgctaat atttttctta atccgatggc gttgatgcta tctacactta
    10741 ttcgtggagc atacttgatt tctttgatcg aatatcgttt tttagggatc gtcataggaa
    10801 gtttcatcgg tttaggtgtt gcgtgggtaa tgacggttgg tttgcaacat tatttaacgg
    10861 ttgttcgtca aaaattagaa tagaaggaca aatatatgtg ccaaaagagg ttatgacaag
    10921 atttttgtca caacctctaa ttcaaggtgt tcaaaagcca gattctcagc attaagtcaa
    10981 attcacacaa aaatgtggaa aggaactttc aaaaaaattt tatatttatt ctatcaaaaa
    11041 attttgttta tgatggtaac tgaataaact ttcctgagat ggtcaatgct ttccttgaaa
    11101 gtcttacaga tattctttct tcatagaaag tttaagtttt atagttagtg tacaataaaa
    11161 gtatgtttat accatatggt gtccggataa tcagtcattt aaacaatgag gttcaaagat
    11221 atgaaaaggg gaaatttgat gaagaaaata agcaatgaaa tccaaattct ggcagaaact
    11281 ttaactcttc ccaaagtcgt tatagagcaa atgattgtac aagcaaaaaa catccttgat
    11341 gaggtgtttg agcaatatag tcatgaacta atggatccta ataaagcaaa ggcgactgca
    11401 aagaaaatag acaaagtcta tgaagaggaa ccttttgcag cattagctat ttatctatat
    11461 gcagcttctc aaagttggaa aaaaatttat caaccattga agattccaag aactatctat
    11521 ttggcaacaa tgaatgcttt tactagattt attcatgaac atttccaagt aacaggaagc
    11581 tatcgctttg accgtggttt ttggatttgg cattacacta gtggcttgat ctttcggatt
    11641 ggtgaattgg agtacgaaat gacatatttt ccgaaagggt acacggatct aagattagaa
    11701 ggaaagcagt gcctttctct tcatattcct tccgatgcag atttatctca gaataagata
    11761 agagatagtt acgaaagtgc cattcgtttt tttgaacttt attttcctga ttaccattac
    11821 caagtgatgt acacagatac ttggttacta tcacccaatc ttactaaata gttgaaaaaa
    11881 gagtcaaaaa tctgtctttt tgcagccgat tatcgattat tatcagttga tgaacaggac
    11941 gatagtggag tcccttggat ctttggacga gtagatgccc aaatacatga ttatccagaa
    12001 agtacgagtt tacagcgaca agcaaaagag caattgcttg caggcgagca cattggctct
    12061 ggcttaggta ttcttacttc ataagtttag tacttcgaaa tagtttaaaa gagcgtgaga
    12121 caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagcagaag
    12181 caactccttc ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattt
    12241 cttcttattt cttggagtta aacgcttctg tcccagcctc ttttttagtg ttctatcgaa
    12301 cacacagtac gttcaatagg taaactagct ataacaatag atattaaagt taacggacaa
    12361 gagggccttt tttaggtagc taaaacgtaa taatgaaaat ttatttccag agataggagt
    12421 caatcagcta gtctgatcga ctgttttaac aagttgactt tcattttaac ttttgatacc
    12481 atttaaaaca caaatcgtaa ttgtttcgat ttgtgttttt tagcatgact acaatcaaag
    12541 aagaaagtgg gtcgtccata tggaaaaaac agatatctca agtgcatacc gccgcttgaa
    12601 aagcccaaat atcaaaacac gaaaacgtgc gctgaagatc atcaaagatg tgaaacggaa
    12661 tagtgggaaa aggtaagaag aaggaaactt aattaaaaaa atagccgaaa aacgtttttc
    12721 ggctattttc aataatcggt ttttgtgtaa ggtttccgaa agaactgctg ttgattttcc
    12781 atttttaaca tatgataaat tggtagaatc tttcttttca aattgatacc attccctaat
    12841 tccagttata tagttaacta cagaaccaca aagcacgtac gtttataaaa gtattatctg
    12901 tagttatttt tcaattccct ttcaacctct tattttgacc agtagtattg atcactttac
    12961 cttttaacac ctttgttcgt ttttgaaaca tttttagtca agaatggaaa cgtaactaca
    13021 taatcaaagg ttttgcgaaa tatttcatga aatatttcat gtagaagcga gcaatacggt
    13081 tgcaaataac cattatcgtt taataagcag ttacgaaagc tcccaatatc tctaacgttc
    13141 agctcactcc atttcttttc ttcaaaaaaa tcagtgattc gtatctcttt ctatttgtcg
    13201 aaaaagttaa ctttaaaaag taacgccgaa aaaatatgca aaaaaataaa aaagcagtta
    13261 ctattgctag aataacatca aggaataata agttttggtt ttcagataat agtcgataag
    13321 ttgtgtattg gaattttaga tacagtaata aagtattttt catgaaattt tgtgagaatc
    13381 actattacaa gtatctttaa taaaattaat ggttaaggag gaacttaaag tagaatgtaa
    13441 atgaatagtt gtagtcagtc gattgtgata ctagactcaa agttgcagga ataagttttt
    13501 caatttgtga ttggaagata aaaaatgaaa aagaagggct tttttttata ctttgttgat
    13561 tgtagtgttt attttccctc tgtttagcca agtaacattt tctatgactc aaccagtttc
    13621 aagtgaggaa catgataacc tttttcgtgc tacagaagag tttaagaaat atcaggaaaa
    13681 tattgttccg gagactccat tagctagtga gccaattact gatattgatg gaaaccaaat
    13741 aggatggtat atacagtacg aagttatggt ggtaaataat gactcaatag taaaaaatac
    13801 agtaggctta gagctaacac cgattgaaaa ggtgagttca ttgttaacgt atatttatac
    13861 tgatgaaagt gacgagactg agatcatttt aacggattac tcaaaaattg ctactgataa
    13921 aaagtatata ttgtggatgt agacactagt gaaatacttt atgatataga aattgatgaa
    13981 aatttactag atgtagcaca agaaatcgat catcaaaagc aggaagctat tcgatatgct
    14041 ggtagaaaag ggaaaagaaa atatttatta cgtgaatgta gcaaaagtta gagaagatga
    14101 aaatgagtgg aaagaattca agagtcgcta ctcaattaac tcgactccga catttacagt
    14161 ttacagagaa ggatcgatag aaaaaactgt tttttggact aaagaaagtg gaatgtcttt
    14221 agctgaagta gaagagtttt tggattatgt tagtatgcaa cagtaaggga gttcttatta
    14281 tgaaaaattt tgacacatat tatgttattg ttttttcgat atctttgctg ttaatattac
    14341 taactttaat gcagtataaa gagtatccag agtattttat tttaactttt gtatatttag
    14401 taagtagcta cacacttcta tataaattca gaagttcttc tagagtttct aaagtttgtt
    14461 tcttaattag cttattgatt c
//
LOCUS       DACCYH010000071        14381 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.074, whole genome shotgun sequence.
ACCESSION   DACCYH010000071 DACCYH010000000
VERSION     DACCYH010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.074"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(27..923)
                     /locus_tag="IVY85_002079"
     CDS             complement(27..923)
                     /locus_tag="IVY85_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8045088.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(916..1596)
                     /locus_tag="IVY85_002080"
     CDS             complement(916..1596)
                     /locus_tag="IVY85_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8045089.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1597..2379)
                     /locus_tag="IVY85_002081"
     CDS             complement(1597..2379)
                     /locus_tag="IVY85_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8045090.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2372..3289)
                     /locus_tag="IVY85_002082"
     CDS             complement(2372..3289)
                     /locus_tag="IVY85_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8045091.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3453..3776)
                     /locus_tag="IVY85_002083"
     CDS             complement(3453..3776)
                     /locus_tag="IVY85_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8045092.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            4564..7791
                     /gene="fss3"
                     /locus_tag="IVY85_002084"
     CDS             4564..7791
                     /gene="fss3"
                     /locus_tag="IVY85_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP8045093.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            7904..8218
                     /locus_tag="IVY85_002085"
     CDS             7904..8218
                     /locus_tag="IVY85_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8045094.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            8231..8605
                     /locus_tag="IVY85_002086"
     CDS             8231..8605
                     /locus_tag="IVY85_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8045095.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            8615..8950
                     /locus_tag="IVY85_002087"
     CDS             8615..8950
                     /locus_tag="IVY85_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP8045096.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            9033..10373
                     /locus_tag="IVY85_002088"
     CDS             9033..10373
                     /locus_tag="IVY85_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8045097.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            10451..11125
                     /locus_tag="IVY85_002089"
     CDS             10451..11125
                     /locus_tag="IVY85_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8045098.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            11358..11792
                     /gene="dcm"
                     /locus_tag="IVY85_002090"
     CDS             11358..11792
                     /gene="dcm"
                     /locus_tag="IVY85_002090"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP8045099.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            11793..12500
                     /locus_tag="IVY85_002091"
     CDS             11793..12500
                     /locus_tag="IVY85_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP8045100.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            12490..12780
                     /locus_tag="IVY85_002092"
     CDS             12490..12780
                     /locus_tag="IVY85_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045101.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            13039..14223
                     /locus_tag="IVY85_002093"
     CDS             13039..14223
                     /locus_tag="IVY85_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8045102.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            14238..14357
                     /locus_tag="IVY85_002094"
     CDS             14238..14357
                     /locus_tag="IVY85_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP8045103.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         4596 a         2632 c         3019 g         4134 t
ORIGIN      
        1 tactagttct cactaatctt gtataattaa accctccgca acttgacgga gatggagaag
       61 atgcccccgt ctaatctagc ggtcatctct cctcccattc cttccaccaa tttttttgac
      121 aggtaaagcc cgattccgct agattccgta tttgtgcggc tggtattttc ggtataaaaa
      181 cgctggaaga ctttttcaac agaggtttgc gttcctggtt tgataggatt ggccactgtt
      241 aatcgaatga atggatcctc ttttgaaaat gtcacctttg cttgtccatc tgcataacgc
      301 aataaattct gtatgatatt ttgcagcact cgcagcaaca tgagcggatc tgctaataca
      361 gttgtattta tcgagtgttc agcgaacgct acctcgatat ttttctcaga aaattgttca
      421 taaaaagaga aaaatacttc ttccacctct ttagaaaaag aaagcgtatg gagatccatt
      481 tctttttgct tcgtctctat tcgagcaaga tcataaaact gatccgtcat actgagtaat
      541 cttgcaacag attgtttgat gatcgttaaa tacttctccc gtttctccgt atcctctacc
      601 tgttccagta attgtacata accgttaata gcagtcaaag gagttctgag atcatgagaa
      661 aggccgacga tcgatagtct gatgttttct tccatttctt gaaatgcttg tcctttttgt
      721 tcattttcat ccaaccatgt attcagtgca tctactaggt ccattagatt cttttctcga
      781 aattcgactg aaagacgacc accatattct tgtcgttcag aaagagtggc gattttctta
      841 gttaatctct tgatttccct ttgcaggaag aaaataaaac ttcccataaa gataacaacg
      901 agaagcaaca gccatttaat caaatctaaa ccccactccc cacaccgtat gaatatacac
      961 agcatgtgcc tttgctagct tccctcgaag attggaaata tgcatattca ctgttttctc
     1021 actatcaaaa tatggttctt gccaaactgt ttcataaaga tttgctcgcg aaaatacttt
     1081 tttcggatgt tctaaaaaaa gcttcagcag atcgaattct cttcctgtca aatggacagc
     1141 cacaccattc acatttacat ccctcgactc tttgtccagt aagatttcct tgtaatagat
     1201 gcgttgcgac agatctttta cttggacaga agtctgatga cgaagttgga tgccgattcg
     1261 taacaaaagt tcttcgatat caaatggttt cgtcacatag tcatccgctc ctgctgccaa
     1321 caaggaaact ttatctcttt gatcgctttt tgctgaaaga acgatgatcg gaacattcga
     1381 tgtctgtcgg atttctgcta acaatgcttc acctgaaagc cctggaagca tgagatctag
     1441 aaggatcaaa tcgacaggat atgtgcttaa caataatttt ccttccgtac ctgaataagc
     1501 atcaagaagt ttgtatctct gttcaagaac ctcctccagt aaggaatgaa cagcttcatc
     1561 gtcttcgata atcaaaatag tttccagcag gaccacctac ttaatgtcag atttttggaa
     1621 aataaacatt cctagagctg atgataccac gattgttact agactgacca gaatcatttt
     1681 cggtgcttgc gaataagttg cttgttgcgc aaagtccgta tacatgagcc acgtttccgt
     1741 aaaccactcc gttagaaatt ccaagccttt gatttttgcg aaaacaccgg gaatgaaggc
     1801 cgttcccatg atataaagaa aagaaagaag caaactgaca ctggtacttc tagtgatgaa
     1861 tgtcaaagcc actggaacag ataatgtccc taatgtcagc agaagcaata caggaaatac
     1921 ttttagaaca taatttccat agtttttgat tccctcaact tcaccaaatc cgaaagcaag
     1981 tgtgtaagca agcatggcta gtcccgtgat caaaaccacg ccaagtgttg tgatcaaaag
     2041 cgtaacgaga agtttcgaca cataataagt cattcgattc cgtcctaaag ataacgtatt
     2101 gcgaatcgta ccgttttgaa attcttcccc aatataaaag cacgcaaatg ctgcgaaagc
     2161 cagaagaatc catgcagcca ttttttctac cattaaaatc cctgttgcac cgttgatgac
     2221 tgtatccgtt gcttggttta attcattgcc gtattttgta tgcatatacc attcgttcca
     2281 agaataaaaa agcggaagta cgaccatcag tcctagtgag acccacattt tacggctctt
     2341 ccatactttt attttttcag cagttaatag attaaacatg tggattgcct ccagttaatt
     2401 gtaagaaata atcttccagt ttttgcccaa ctaggtgaat accatccaca ggaacttttg
     2461 cttctaccaa tgcttgattg atggctgcta cttggtcaat tccttcaaaa agctggataa
     2521 cacggctaga ttgtacaaaa taatccctat aaccaagaga gtcgagaacc gttactgctt
     2581 gttccgtcgc cgaagtgtca atttttataa attgacgaga ttcttgtgcc aattcttctt
     2641 tcgataattc tttgatcaag tgtccttcat gcaaaatacc atagtgagtg gcgatctgtg
     2701 acaattcatc tagcaagtgg ctagaaagca agacggtgat gccccgttct tttaccaaac
     2761 gttggatgat ttctcgcatt tctgccatcc ctgctggatc catcccattc gtcggttcat
     2821 ccaaaatcaa aaattctgga ttcgtgatca gtgcatttgc aatagctaaa cgctggcgca
     2881 tacctaaaga gaaattcttt gctttctttt tccctgtatt ttccaatcgc atcattttca
     2941 gcaggtcttc gatttctcta tccgacacac cgccgttcgc tgcttggata cgtaggttat
     3001 ctcttgcagt taattctgga taaagagcag gggtttcaat cgattgaccc atacgtcgtc
     3061 ttgctgcctg caaatccgtc gttccaaata attcgatcga accttcatca atcgagatca
     3121 aacccataat agttctcata aaggtagact tacccgctcc attttcccca atcaatccat
     3181 agatcattcc acgtttgatt tcgattgata ctttatctaa tgctttggca gcaccgtatt
     3241 ttttggttat accagttgct ttcaagaccg tttcatttcc aaaatccata cttatccttc
     3301 tttcttattg atgagtgaag tatagttgac gggtataaag aaagcagcca gaaatgtata
     3361 aagaaagtgt aaattttcca attgaatgtc aaaaaaaaca atcggacaat acgtgtcttt
     3421 cctattatca ggcagtttgt tatacgcttg tatcagtttc ctgattcttt cttttcgacc
     3481 aaagtcgttt ctctgtattc ctcttcactg aaaatttttg cttctttcag atcaagcttg
     3541 agtaatttta ccagaccggt tataaggata actatagcaa gactccctcc cagaaaaatt
     3601 agactgtctc gcagacgtgg ttgcgttgtc ttccaatcaa agagcgcacc aaacagtgct
     3661 aatccaagtg gactggccac catggtaatc gttgttatcg ttgacataag tcgtccttgt
     3721 atatgcgttg gaacttctcg ctgaagccag accatataaa aagcaaaaga aaacattaat
     3781 ccttaaaaac ggacttatga aaaataatac atgccgtttg ggatttacaa ttagctacca
     3841 aataagaggt tatgacaaaa gacttttcac aatcccttct tccgaataaa cggtgttctc
     3901 ctgccaactc ctcggtcaca agtcacaata tttatcaaac atgagaagca atttttgtaa
     3961 attccttctt atttcttgaa gctgaccact tctgtcacaa cctcagaagc ggataaataa
     4021 tattgtttga tgatacttta tctttttccc gtttgtttat agatcgaatg atattataga
     4081 ccaagaatcc tacctattaa catagctaaa aacacaaaaa aggcactttc caaattgtgg
     4141 aaagtgccag aaaagttagg attaaggtaa ctgcgctcag cattgcttcg cccagtacga
     4201 ttctcgtaac tcgtgaactc gaacgataat cgcaattaac gttttgagaa ctgtgaagct
     4261 ttacgggctt tcttaagacc tggttttttc ttcgtatcat tgaataagtt tgaaaaacct
     4321 tgatttataa aggttttccc gtaaatatat tttattatat ttgattaaat ttgaaatgtt
     4381 tgtgttctaa aatgtgttct aatttaaagg cagtatattt cgtattttag attaaatttt
     4441 taggctcatt cattctcaaa tccattctat tcagtcaata aaaattttaa aaagacttta
     4501 taaacaaaga tgttcaagcg agcatcttat ttttttgtgc ttaatttgaa aggagaacat
     4561 ttcatgtata aaaaacaaaa aaagaaatta tgtgtattaa ctattttgag tttggctgtt
     4621 caagcaattg ttggagcaat tgctcctacg attacttttg ctgatgaaat tacccatcca
     4681 caaacggtaa ccgtagaact agatttagca catcaatatg ctgttgaagg aacttttagt
     4741 gatggtcgtc caatgtcaga agtcactgtt ccgcattatg cagtgtacaa cggctttaaa
     4801 caagatattt tctgtattga accaggagtc ccgatttaca atgagttcac tcctggctat
     4861 gagaaaaacc cgttaccaga tatgtccgat aaagccaaat tagtttccgt tttatggaaa
     4921 aaagtaggta cagatgcaga tactcatatt gtggcacaga agatgatctg gcaagaagtt
     4981 aatgggtata cccttcactc aataaaacga tcagatggta gtgccgtaaa tatcgcagcc
     5041 attgaggcaa aaataaatca agccattgac gattatcaga agaaaccaac cttccataat
     5101 agtacggcta aaacagtatt agggcaatcg actactgtga cggatacgaa taatctgaat
     5161 ttgtcagagt tcgatgaggt tgtggaaaat gccgcaaaca ttgattatcg tgtgaatggt
     5221 aatcagttga taatcacccc aaatgccaat tcaaaagaaa gtggtgtgct gacgcttaag
     5281 aaatctgctg gtaccggaac acctgtcgcc tataaaaagg ctggccaaca gactttaatg
     5341 gccggggcga ttgataaacc taatacctac acaatcaaaa ttgatgtgga aacggagggt
     5401 tctttaaaga tcaaaaaagt cgataaagaa tcgggtgatg ttgtaccagg aacggttttc
     5461 catttagact ttggaaaaac tttacctgca aaagacgtga ctactgacaa agaaggcatt
     5521 gctacattgg atgagattcc tcatggtaca aaagtaacca ttaccgaaaa atcagtaccg
     5581 gcgccttata cgattgatac cacccccatg actaccacca ttaaagcagg cgaaaccatt
     5641 tacgtgactt caaaaaacgc acgggaaaaa gggcaaatca ttctggataa aagcggagtt
     5701 gaaacaggga gtgatctttg gaatgacaat tattccttag ctggtaatac gtttgccatt
     5761 cgcaaagaca gtccaaccgg tgaaatcgtt caagaaatga ccacggatga aaacggtcat
     5821 gcggaaacac caaaagagat tgctaatgcg ttggaattgg gaacctatta tgtgaccgaa
     5881 actaaagcta gtcatgggtt cgtgaatacg ttcaaacctg taaaagtcga attgaagtat
     5941 gccaatcaaa ccgtagcccc tgttaccagt aacgtaaaag ggcaaaatca agaagtcact
     6001 ggcgaaacca ctttgacgaa agaagacaaa gatactgggg ataaagcaca aggtaaggca
     6061 gtctttgagg gtgctgaata caccttgttt actgctaagg atggaaaagc tgttaaatgg
     6121 agtgaggctt ttaaaccaga gttagcgaag ggaacgaaag gttctgatga aacggtcact
     6181 ttgactttag acgaaaagaa ccaagttgcc gttaaacatt tagctatcaa cgagtattat
     6241 tggcaagaaa caaaagcgcc cgaaggatat agccttgatg aaacaaagta tcctgtatcc
     6301 atcaaaaagg tggatgatag cgaaaaaaat gccgtgatta cccgaaatgt tacggcaaaa
     6361 gaacaagtga ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgcgggcact
     6421 accgaaactg gcttcaatga tctgaccttt aaagtgtcgc cattggaagg gaccaatgaa
     6481 atcacaggtg ccgaagatga agcgaccaca gcttataacg agcaattagg gtttgatggt
     6541 tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt agaagcccca
     6601 gaaggttttc aaaagattac gccattagaa atccgttcta cttttaagga aaacaaagaa
     6661 gactttgtga agagtgagta tgtctttacc attaccgaac aagaccaaaa acaaccaatt
     6721 aaaacggtga ccgttcctta cgagaagctg acgaacaaag cattttctgt tagtttgaac
     6781 cgtttaatgc tctatgattt gcctgaggaa gaagatagtt taacttctct tgcgacttgg
     6841 aaagacggaa acaaagaatt aacttctctg gattctaccg agctagttga taaattgagt
     6901 tacaatctac atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggat
     6961 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtgat tgcagaaaca
     7021 agcgcaacgt tggcgaacaa agagaaaaca ggaacttgga aaattcagca taaattaacc
     7081 gccgaacaag ttttaaataa aactattgtc ttattcaact atgtgtatga aaataaggaa
     7141 gcttttgaag caggcgataa gccagtagcg aaggatgtta gcttgaataa ccaagcccaa
     7201 actgtcagtt gtacagtgga acaccatgtt tctatccaaa caaaggccca cctagaaaac
     7261 ggttctcaga cctttactca tggtgatgtg gtggatatgt ttgatgatgt gtcgattact
     7321 catgatgtac tggatggctc aaaagaagct ttcgaaacaa ttctgtatgc tttattaccg
     7381 gatggtacaa acaaagaaat ttggaaatct ggcaagattg attatgaagt aaatgataaa
     7441 gaattcacca aaactgtgct tgcgaaaaaa gtagataccg gaaaatatcc agaaggaacc
     7501 aagtttactt ttgcggaaat caattacgat aaagatggga atatcaatgg aaaacacaat
     7561 gaagatttga aagaaaaatc tcaaacctta acaccaaaag aagttccaac catactaagt
     7621 acgccgaaac aaccggaaac accaactgtt ccaagtggct ctcaagaatc tagtcccaca
     7681 gtgaagacct tccctcaaac cggggagaaa aattccaatg ttttactgtt cattggtttt
     7741 actttgattt ttgcgacggc gggctattat ttctggaatc gccggaacta aggtgatgag
     7801 atgatacaag taaatcctaa gaaccaacgt gcgcgaccgc ctcctaaaaa agtagtcgcg
     7861 ctatttttgt atccaaaact aacaatgaaa agaggaaaag aagatggaat tgaaatttgt
     7921 cgtgccagat atggctgaaa cctttggaaa gttaagttat gccggtgaag gagaaattat
     7981 aacagaagga tatggacgga atactacggt gattggtcgc agttatcatt tgtattccag
     8041 taagcaacgg gccgatgata ttgaggtggt agtagctgcg gaagccggtg aaaaggattt
     8101 tgatcaagac caaccgctaa aagccgtgaa tccccatttg gttgccaaag gctatgagat
     8161 tgagaatcgt gggtttactg attacgtgtt gtatgttgat gatttagtga aagcatagga
     8221 ggaaaagaag atgagattaa cagaaggtat tgtcgtagat tcaggtttaa catttgggaa
     8281 gttgcgtttt tccgcattac gccgagaagt acggaagcaa aatgaggatg gaacaattgg
     8341 taatgaggta aaagaacgaa cctataattt aaaatcctct gcacaagggc gaatgattca
     8401 agttagtatc ccagctaatg tacccttacg cgagtttgct tatgatgcag aggtagagtt
     8461 ggtcaatcca atcgtggata ccgttgccaa ttatgttttt cgagaaggaa ctaccgttaa
     8521 ttggttcatt aaggcggatg atttagtctt gaagcggcag ccgaatcaag gaaatcctac
     8581 gaaccaaaat gaaagaaaga aataggtgaa gattatggaa attaatgttg aacatatttt
     8641 agattgtctc gatcaatatg gaaaaggcga gctgactgag gaacaactcg ttaaagcatt
     8701 aacctctgat gaaaaaatgt ttctgattat acatcaaggt ttacttggag aaaatagcga
     8761 cagtgaagaa gactctgatg tactagactg gttaggagaa gaagagtctt tctttatagt
     8821 tgtcgaagta aatgaaaact tatgtcggca ggcagaatcc attttggaag aaattggtgt
     8881 ggagatgccg gatgctattg aaggcttttt aaatcaattg gttgaaacga aacaactccc
     8941 ctcctcgtag cagtaaatga ctagcataac tttttgaaat tgcctaatga aatcctattg
     9001 ggctttttcg attggaggtg gaaaccatca aaatgtacaa aggacatcgc atacgcgcag
     9061 gagatcagca cttggtttat cactttgttc ttggctggct actcgctctg ttcatcggtt
     9121 ggatgagcgt cttttatttt caagagttta gacaatttga tatttctaag ctatcacttt
     9181 ctactattga aatcgtttgg tctataaaag atttagtatg tctgcttgga agtctcgctt
     9241 tttcaggagc tatgatattg ctttatattc acttttttct tgatcattgg cgaagcttgt
     9301 ggcatcgaca aaagctagct cggatgattc tagagaacca ttggtatgaa gtgaaacaaa
     9361 ctcaaagtga aggctttttc aaagatttga atagtagccg aaccagagag actatcagtt
     9421 acttccccaa aatctgttat cgaatgaaag atggattact gtctattcgc gttcaaattt
     9481 cacttgggaa ataccaagat cagcttttaa aattggaaaa gaagttagaa agcggactgt
     9541 attgtgagtt agtagaaaag gaactcaaag actcctatgt ggagtatact ttactgtatg
     9601 atatgatcgc caatcgaatt gggatagacg aagtagtggc agaaaatggg actctgcgat
     9661 tgatgaaaaa tcaagtatgg gcttatgatt ctttacccca tatgttaatc gctggtggca
     9721 cgggtggagg gaagacctat ttcctcctca ccattatcga agcactattg aagtcagatg
     9781 cagaattgtt tatcctcgat cccaaaaatg cagatttagc tgatttaggt acggtgatgc
     9841 ctcatgttta ttcacaaaag gaagaaattt ctgcttgtgt ggaagatttc tatgaacgta
     9901 tgatggctcg tagcaaggca atgaaagaaa tgtctaacta caaaacagga gagaattatg
     9961 cctatcttgg acttccaccc aactttttaa tctttgatga atacgtggct tatatggaga
    10021 tgttaacgac gaaagaaagt gcggtgattt tgaataagct caaacaaatc gtgatgttgg
    10081 gtcgtcaatc tggtttcttt ctgattttgg cttgccaaag gccggatgcg aaatatttag
    10141 gagacggaat ccgcgatcaa ttcaactttc gcgtggcttt gggtcgtatg agtgaactcg
    10201 gatattccat gatgtttggg gaggtcgaaa aaaacttctt tatgaagcac atcaaaggtc
    10261 gaggttatgt ggatacaggt ggcagtgtga ttagtgagtt ttatacgcca cttgtaccaa
    10321 aagggtatga tttcttagat tcaattagta aattagaaaa gatgaatgat tgagtgatct
    10381 tcaatactga ctttatactt caagtaacgt ataatacttt tatatagtag gaagaaaggg
    10441 gataattaat atggaaagta atgattctta tttagagtat gcaaaagcaa ataaggataa
    10501 gtttatttct tatattatca aagggaaatc ccccgaagtt ggacgagacg cagtttttat
    10561 ggcagggagt cctggggctg gaaaatcaga ggttgccatt ggattagctg agaattatga
    10621 taaccatgtg attattgatg cggattactt tcgcacccag tttcctgatt ataatggaaa
    10681 aaactctagt ctctttcaaa aagcatcttc ttggttggta gagcaggcat ttagatatgt
    10741 cttagatcat ggctattctt ttattctgga tgcaactttt gcaatattaa gtgctgaaaa
    10801 aaatatctcg cgggcgttga aaaaacattt tcgagtgacg attttctatg tttatcaaga
    10861 ccctaaaatc gcttggaaat ttacacaaga acgtgaaatt gcagagggac gtcatgtgcc
    10921 gaaaaaggca tttattaacg cttttttcaa atcaagagag aacattgcaa aagtaaagag
    10981 taaacatcca gatgtattgc ttcatatcat aataaaagat tatacgaata atatttccga
    11041 agttcattat gctacggata atctagaatt atttttacca ctagggtata gtaggaaaga
    11101 tttggaggtt caattacatg actgagaaag aacaagtaac gaaaattgta aagaaataca
    11161 ataagagcat tgcggattta tcggaaaatg ctactgcaaa agagttcaaa acggtgatta
    11221 aatatgtagc cgatcaggcc aatgaaaaac aacggaaatt agttggatta aataaaaaat
    11281 agtaggacaa attaataatg tatgagccaa gagcaaaatg ttcttggctt ttttctttgc
    11341 aatgaatgag gtaatgtatg agatttttag atttattcgc cggtattggt ggctttcgtt
    11401 taggtatgga acaggcgagc catcattgta tcggtttttg tgaaattgat aaatttgcta
    11461 gacgtagtta taaagcaatt catgatacaa gtaaggaggt ggaaatgcat gacatcacaa
    11521 gtgtatcaga tgagtttatt caatccctcg gaccagtgga catcctttgc ggcggatttc
    11581 cgtgccaagc tttttcaatt gcgggaaagc ggcaaagatt ttccgatact cgaggtactt
    11641 tattctttga aatcgctcgg ttcaccactc ttctccgacc taagctttta ttccttgaga
    11701 acgtccgagg attgctcaat cacgaaggag gggctacgtt cgagacgatc ctccgaacgc
    11761 tggatggatt ggggtatgac gtggagtggt aggtgcttaa ctcaaaggcc tacattcccc
    11821 aaagccggga gcggatattc cttatcggac attctcgaga cgcatgtacc gaacaagtat
    11881 ttcctatcat tggaccgtct tcaacatctg atcaaaacat cagaaatttg gtaaatatca
    11941 atccttccaa tcgaggaatg ggtggacaag tctatggttc agatggcgta gcgccaacct
    12001 taacaggtga tgaaggaatc aaaatcgcac tgccagcaag ggatggaatc tctgtagcag
    12061 gactattacc gggaaagttt gaacaaggta atcgagttta tgaagttact ggaaccgccc
    12121 ccaccttgtc aacgaaacaa ggtggtacaa aaatcatgat tcgaaaaaat gaaacagaat
    12181 atcaagaggt gaagccagaa gacagtgtga atctggcttt tcctaattcc acaagtcgcc
    12241 gaggtcgtct gggaaaacag agtgttcata ccttactaac aggagaccaa caagcgatcg
    12301 tcacggatca gtatcaaatt cggaagctaa cacctagaga gtgttggcga cttcagggct
    12361 ttcccgattg ggcttttgat cgagattctc aagtcaattc agatagtcag ttgtataagc
    12421 aagccggaaa ctctgtaaca gttccggtca tttttgatat tgccagaaga ttaaaagagt
    12481 tggatgtaca tggtttatga tgagttgatt ggagaaattt attgggtgat tggaaagatt
    12541 cagtcagacc cagaattaga agaacaattg cgacggttaa aatttgatat tcgcaagaat
    12601 ggtgtcaaag tcgctggcga tccatattta atgaatgagg agacggatgc tcgaattgaa
    12661 ataaatcaag taattgcaga atttgagcgg attgcggacc aagcaaaaga accagatatt
    12721 cgtcaatact tgttagaaat gaaagcagag ttggaagtaa atggtattac agatgagtaa
    12781 cctaaaccaa cacaactgac tgttaggcgc tgaccgcgga ggattcgcga agcagaaatc
    12841 ctccgtggtg ctgtgcctgt tcatggacgg cgttagccga ccatgatgtc tcacccccgt
    12901 atctaacagg ggggtacaaa aacacaaaaa ataagtaagc aaccattaag aatgcttgtt
    12961 gtatcaaaga tagtaagtaa tttgtatagg tgttaactca tttgaaaaag ttaacacctt
    13021 gaatttggag gtgagaactt ggcacaacgg aatttagatt atcggctatt aaaagaccga
    13081 cgcaacgaat acggcgtttc acaaaataaa ttggctacgg cttgtggact tagtcggccg
    13141 tacttaaatc agatagaaaa tggtggcgta accgcatcta taaaaaccat gaggaaaatt
    13201 tttgaccaac tggaaagctt taatcccgat ttgcctttga cgttactgtt tgactatgta
    13261 aggattcgct ttcccacaac ggatgcacga aaaattattc aagaaattct ccatttgaaa
    13321 tttgagtata tgctccatga agattatgcc ttttactcct atcaagaaca gtatgtcatg
    13381 ggtgatattg tggtgatgtt gtctcatgaa gaagataaag gtgtgctttt agaattaaag
    13441 gggcgaggtt gccgacaatt tgaaactttt ttactcgctc aaaagcgtag ctggtacgac
    13501 tttttcgagg attgtctaaa ggccggtggc gtgatgaaac gcttggactt ggcaatcaat
    13561 gatcgagtgg ggctattgga tatacctgag ctaacgaaaa aatgtcagaa ggaagaatgt
    13621 atctccttgt ttcgtacctt taaaagttat cgttccgggg aacttttaaa ggctgatgaa
    13681 aaggacggca tgggcaatac cttatatatc ggcagtctca agagtgaggt ttatttctgt
    13741 ttgtatgaaa aagactatga acaatatatc aagttaggga ttccattaga ccaaactgag
    13801 acgaaaaatc gttttgagat tcgactgaaa aatgaccgtg cttttcatgc gattcaagat
    13861 ttgttaaaag gtcgtagcat tgagagcacc acgttttcca ttatcaatcg ttacttgcga
    13921 tttgcggata aagtagaagg taaaaggcga accaactggc ccttgaatga acaatggggg
    13981 cgttttattg gacggaatcg gaaagagatt caattaactt ccgagccaaa accgtatacc
    14041 attgaacgaa ccttgaattg gctaggccga caagtcgccc ctacgtggaa aatggctaag
    14101 gagttggatc gattgaatcg gacaacctat atccaggata tggtacagaa tgcacgctta
    14161 tcagaccgac ataaaaagat tttggagcaa caaagtatgg caattgaaaa tttgattata
    14221 tgaaaagagg aatcgtcatg ggaattgtaa aagatatttt tttggtattt ggtggcagta
    14281 tgcttggtgt tactgtgatg tgcttgatga atgcaagtgc agcagcggat cgagcaatgg
    14341 aaaagaaagg aaagtgacaa gatgaatttc ggacaaaatt t
//
LOCUS       DACCYH010000072        14276 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.075, whole genome shotgun sequence.
ACCESSION   DACCYH010000072 DACCYH010000000
VERSION     DACCYH010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.075"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..202)
                     /locus_tag="IVY85_002095"
     CDS             complement(<1..202)
                     /locus_tag="IVY85_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HAP8045104.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPA
                     TRLGFALQLVSLRFLGTFILDLT"
     gene            327..932
                     /locus_tag="IVY85_002096"
     CDS             327..932
                     /locus_tag="IVY85_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8045105.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(998..1630)
                     /locus_tag="IVY85_002097"
                     /pseudo
     CDS             complement(998..1630)
                     /locus_tag="IVY85_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
     gene            1919..2311
                     /gene="merR"
                     /locus_tag="IVY85_002098"
     CDS             1919..2311
                     /gene="merR"
                     /locus_tag="IVY85_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HAP8045106.1"
                     /translation="MIYRISEFADKCGVNKETIRYYERKNLLQEPHRTEAGYRIYSYD
                     DVKRVGFIKRIQELGFSLSEIYKLLGVVDKDEVRCQDMFEFVSKKQKEVQKQIEDLKR
                     IETMLDDLKQRCPDEKQLHSCPIIETLT"
     gene            2340..4235
                     /gene="merA"
                     /locus_tag="IVY85_002099"
     CDS             2340..4235
                     /gene="merA"
                     /locus_tag="IVY85_002099"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HAP8045107.1"
                     /translation="MNKFKVNISGMTCTGCEKHVESALEKMGAKNIESSYRRGEAVFE
                     LPDDIEVESAIKAIADANYHPGEAEEFQSEQKTNLLKKYRLNVEGMTCTGCEEHIAVA
                     LENAGAKGIEVDFRRGEALFELPYDVDIDIAKTAITDAQYQPGEAEEIQVQSEKRTDV
                     SLNDEGNYDYDYIIIGSGGAAFSSAIEAVTLNAKVAMIERGTVGGTCVNVGCVPSKTL
                     LRAGEINHLAKNNPFVGLHTSASNVDLAPLVKQKNDLVTEMRNEKYVNLIDDYGFELI
                     KGEAKFVNENTVEVNGNQITAKRFLIATGASSTAPNIPGLDEVDYLTSTSLLELKKVP
                     NRLTVIGSGYIGMELGQLFHNLGSEVTLIQRSERLLKEYDPEISEAITKALTEQGINL
                     VTGATYERVEQDGDIKKVHVEINGKKRIIEAEQLLIATGRKPNTESLNLHAAGVEVGS
                     RGEIVIDDYLKTTNSRIYSAGDVTLGPQFVYVAAYEGGLAARNAIGGLNQKVNLEVVP
                     GVTFTSPSIATVGLTEQQAKEKGYEVKTSVLPLDAVPRALVNRETTGVFKLVADAKTL
                     KVLGAHVVAENAGDVIYAATLAVKFGLTVGDLRETMAPYLTMAEGLKLAVLTFDKDVS
                     KLSCCAG"
     gene            4395..5717
                     /locus_tag="IVY85_002100"
     CDS             4395..5717
                     /locus_tag="IVY85_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8045108.1"
                     /translation="MSDLLSLPDIKTIEPPQENETDMMFKVEAVGPPERCPECGFDKL
                     YKHSSRNQLIMDLPIRLKRVGLQLNRRRYKCRECGSTFWERLISVDEKRSMTKRLLKS
                     IQEQSMSKTFVEVAESVGVDEKTIRNVFKDYVALKEREYQFETPKWLGIDEIHIIRRP
                     RLVLTNIERRTIYDIKPNRNKETVIQRLSEISDRTYIEYVTMDMWKPYKDAVNPILPH
                     AKVVVDKFHVVRMANQALDNVRKSLKAHMSQKERRTLMRERFILLKRKHDLNERESFL
                     LDTWLGNLPALKEAYELKEEFYWIWDTPDPDEGHLRYSQWRHRCMSSNSKDAYKDLVR
                     AVDNWHVEIFNYFDKRLTNAYTESINSIIRQVERMGRGYSFDALRAKILFNEKLHKKR
                     KPRFNSSAFNKAMLYDTFNWYEVNDHDITDNFGVDFSTPIKNLEKGDL"
     gene            complement(5844..7349)
                     /locus_tag="IVY85_002101"
     CDS             complement(5844..7349)
                     /locus_tag="IVY85_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepS protein"
                     /protein_id="HAP8045109.1"
                     /translation="MKVSDVISVILKSGIREYKCLNSKIKPIGYKAEGKKGAIFGFRS
                     KKLMTESRGIVLTSQEGLAENEDKFTHWTPNVYSYGAYADEKRIIVKGHNERNLQQIN
                     TFVIDFDRLTGEKLDSQMILDAAIDLELMPTLILETPGGFQAYFILENAWYISSKNNY
                     QSIEVAKRVSENLRKAFAEELPSVDLGCNHFGIARIPRTDNIVYYYPALTHDIQQLIQ
                     WSMKYEPAKTYKKPLLSLVKNKYKQVNEMWYRQLISNPKIVGAKGIMGRNNVIFTLSL
                     ANYASGVDLEDCLNEMDLFNSSLERPLKDRERVRIVKSAYSGKYRGASKTYVQELVSN
                     WGLESLSEEKMFTQQRNTWYKFKKERHKRKKSHLSEWKEDILAYLETQCYRYRPEVRL
                     KKTDLQAAITFNGQSIPKRSLDRALKELMAEGKLFVQIKAGRNGGLVVATRKALIRTV
                     IQVKQQVKSAYKQGIKTFFKEADMLVRIFREPDKYSQRVDHYKQLNLWNTG"
     gene            8053..8943
                     /locus_tag="IVY85_002102"
     CDS             8053..8943
                     /locus_tag="IVY85_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8045110.1"
                     /translation="MANTIIFGQQKGGVGKTTDTCMITLVAAMLMKKRTILIDIDLQA
                     NATNFMTKTFGVETVPQTLMSALENGDLRPAIVNLKENLDLIPAGYDMRLYTEFLTEN
                     FDNTIDRTFYLKNLIEEIKDDYDYIFIDIPPSTDLKVDNAMVACDYLVVVQETQQFSF
                     EGSQRLIFDYIQTLVDDFGSDVPIQIAGILPALLQQKRPMHQKLVEETIDTFGRDNVF
                     NTIIQNHARLEWYPRIGFQFQDYHDKKMIALFADIFCELEERITLYKSNGDIQDYFYT
                     PRYILDGKLTSLGKEISLNV"
     gene            8936..9220
                     /locus_tag="IVY85_002103"
     CDS             8936..9220
                     /locus_tag="IVY85_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045111.1"
                     /translation="MSSLINRKIPQGATANKEADTKRKVTIQKKSSKKILASERKNIK
                     VSPSTFDLIKTISTMKSYKNYEFIDKAVESYIQNNLTEREQRILKNLISK"
     gene            complement(9253..9492)
                     /locus_tag="IVY85_002104"
     CDS             complement(9253..9492)
                     /locus_tag="IVY85_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010621028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045112.1"
                     /translation="MEQSDNVPLDRHKCKEHVFKDKRFVEKESESLTLLGYKIVVDTV
                     TGVNYLETPKGGITPLLDKNGKICIDKKKSINKKG"
     gene            complement(9888..10085)
                     /locus_tag="IVY85_002105"
     CDS             complement(9888..10085)
                     /locus_tag="IVY85_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8045113.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYKQKQGEYVAITIIKE"
     gene            complement(10119..10418)
                     /locus_tag="IVY85_002106"
     CDS             complement(10119..10418)
                     /locus_tag="IVY85_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045114.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            complement(10459..11076)
                     /locus_tag="IVY85_002107"
     CDS             complement(10459..11076)
                     /locus_tag="IVY85_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8045115.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            complement(11401..11796)
                     /locus_tag="IVY85_002108"
     CDS             complement(11401..11796)
                     /locus_tag="IVY85_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045116.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            complement(11876..14227)
                     /locus_tag="IVY85_002109"
     CDS             complement(11876..14227)
                     /locus_tag="IVY85_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP8045117.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
BASE COUNT         4699 a         2556 c         2293 g         4728 t
ORIGIN      
        1 tggtaagatc taatataaaa gttcctaaaa aacgtagtga gactaactgt agtgcaaaac
       61 ctaatctagt agctggtcgt cgtatagtct ccatcaattc taaatctttt tcatgcaaat
      121 aaaaaaattg gtttaattgt tctgtactag gttctcctaa ataaatgcca aatgcttttt
      181 tgttactttt gatttcatcc atttaaaccc tccatgaagt aaattttaag tctacataaa
      241 atttattttt aaaaatgcta tgatagcagt aaaactacaa gtaaatttta catgtaaatt
      301 atatcacttt taaaaaggag ttttttatgg gaaaaattgg ctatattcga gtatcttcac
      361 aagatcaaaa tttggatcga caattggcaa tgatggaagg acttaacatt gataaattat
      421 tccaagaaaa agccagtggt aaagatactg agcgtcctga gtttcaaaaa ttactaaatt
      481 atatccgtga aggtgattgc gtcatcgtta catcactaga tcgtttagga agagattatg
      541 aagatataaa aaatacagtt gcttttatga aacagaaaaa agtagcgtta aaaatattag
      601 atgcacagtt tttagatttt aatactggaa atgaactcct ggataccgct atgttcgata
      661 tgtttttatc actgctaagt tatattgcac aaaacgagcg tgagaaaatt cgtgaacgtc
      721 agcggcaagg agtattattg gctcaaaaag ccggccgata taagggacga ccaatcgagt
      781 attctattaa ctcaacagat ccgcaaaaac gcttagtttt taaaaccgtg gtggacatgt
      841 taaagcaagg cattccagtt gcagagattg caaaagaaaa cgggatctct cgaccaacta
      901 tttataaaat aaagaaagcg aatgatttgt agaaatatac tacattcatt cgcttttcta
      961 ccgcactatt tcgtttctat gttccatgta ccccgatata gggtccatta taaatcacac
     1021 tatcagagtt cgtaccatat ctatctcctt ctttttcaac aacttcctgt ttttgaggaa
     1081 aaaatgctgt attaattagc atagaagtga aatatgggat tggcgtactc aaattaataa
     1141 ccaaagtatt cttatcctta gcataaactc ccaacttatc tggtttcata gttcctgaga
     1201 ttatttcttt accattttta atagattcaa aaaaaccaat atttggtgaa tcttgattac
     1261 ttaacgttct tttccacgaa taaacatagt catctgcaac aacaggcgta ccattactcc
     1321 acattgcatc ctttctcaat cgaataacgt acttaagtcc atccttagag atttctggaa
     1381 ggccattagc tcctccaggt acaatttcac ctttagcatt aagtttatat aatccttcca
     1441 ttacgttatt taaaacaagt tggcttgtag ggtcgatagc taacttcgga tctaagcttt
     1501 gtactgtagt agaaacagca aaagtataag ctttggtttc ttttgctgtt tcttgagact
     1561 tttccccaca accagtaata attattccta acataagaat tgttaaattc tttccccaag
     1621 ttcttttcaa aaaaacaccc ctattcaatt tcttatctga ttgttctagc aacacttagc
     1681 ttaattatac tgtaaaaaaa acgggtcttc ggaatatata gtggaattac tttcttaata
     1741 gtttaaaaac aactggtgtt taaatcggat tttctggtgg gatgttagga taatcaatag
     1801 tattatataa aagtatcaat cacatacgag tgatactttg ggaaaaaaat aaaatagtcc
     1861 aatttacttg aacgtgtact atggtacacg gtttataata ttcatggggt gaaataagat
     1921 gatttatcgc attagtgagt ttgcagataa atgtggagtt aataaagaaa cgatcagata
     1981 ttacgagcga aaaaatttat tacaagaacc tcaccgaacg gaagctggtt atcggatata
     2041 ttcatatgat gacgttaagc gtgttgggtt tattaaacga atacaggaac ttggtttttc
     2101 tttaagcgag atttataaat tacttggtgt tgtagataaa gatgaagttc gttgtcaaga
     2161 tatgttcgaa tttgtttcta aaaaacaaaa ggaagtgcaa aaacaaatag aggatttaaa
     2221 acgaattgaa actatgttag acgacttaaa acaacgatgt ccagatgaaa agcaattaca
     2281 ttcgtgtcca ataatagaaa cattaacatg agagattaat taacgaaagg agctttttta
     2341 tgaataaatt taaggtaaac atttcaggaa tgacttgtac gggttgtgaa aaacacgtag
     2401 aatcagcact tgaaaagatg ggtgctaaaa atattgagtc tagttatcgt cgtggtgaag
     2461 cagtatttga actgcccgat gatattgagg ttgaaagtgc aataaaggcg attgctgacg
     2521 caaactatca cccgggcgaa gcagaagaat ttcaatcgga acaaaagacg aatttattga
     2581 aaaaatatcg gctaaacgtt gaaggaatga cctgcactgg ttgtgaagaa catattgcgg
     2641 ttgctcttga aaatgcaggt gcaaaaggga ttgaagtaga ctttcgtcgc ggagaagcac
     2701 tgtttgaact accgtatgat gtagacattg atatcgcgaa aacagcgatt actgacgcac
     2761 aatatcaacc gggcgaagca gaagaaatac aagtgcaatc ggaaaaaagg acagatgtaa
     2821 gtttaaatga tgaaggtaac tatgattatg attacatcat catcggttct ggtggagctg
     2881 ccttttcatc tgccattgaa gccgttactt tgaacgcaaa agtggctatg attgagcgtg
     2941 gaacggtggg tggaacttgc gttaatgtcg gatgcgttcc ttctaagacc ttattaagag
     3001 caggggaaat caatcatcta gcaaaaaata atccatttgt gggattacac acttcggctt
     3061 caaatgttga tttagcgcca ttagtaaaac aaaagaatga tttagtaacc gagatgcgaa
     3121 atgaaaaata tgtgaattta attgatgatt atggttttga attaataaaa ggtgaagcaa
     3181 aattcgtaaa tgaaaataca gttgaagtaa atggcaatca aatcacagcc aaaagatttt
     3241 taatagctac aggtgcttct tcaactgcac ctaatattcc cggattagat gaagtagatt
     3301 atttaacaag cactagctta ttggaattaa agaaggttcc aaatcgtctt accgtaattg
     3361 gttcaggata tatcggcatg gaattaggac aactatttca taacctcggg tcagaagtca
     3421 ctttgattca aagaagcgag cgtctattaa aagaatacga tcctgaaatt tcagaagcca
     3481 ttactaaggc cttaacagaa cagggaatta atttagtaac aggtgcaacc tatgaacgag
     3541 ttgagcaaga tggagacatt aaaaaagttc atgttgagat aaatggtaaa aagcgaatta
     3601 ttgaagcaga acaattgcta attgccactg gaagaaaacc aaatacagaa tcattaaact
     3661 tacatgcagc aggcgttgaa gttggttccc gtggtgaaat tgtcattgat gattatctta
     3721 aaacgaccaa ttcccgaatt tattcagctg gagatgtcac tctcggtccc caatttgttt
     3781 atgtagctgc ttatgaaggt ggacttgctg ctcgtaatgc aatcggagga ctaaatcaaa
     3841 aggtcaattt agaagtggtt ccaggcgtta cgtttacttc tccatcgatt gcaacggttg
     3901 gtttaacgga gcaacaggca aaagaaaaag gatatgaagt gaaaacatcg gtattgccgt
     3961 tggatgctgt tccaagagcg ctcgttaatc gggaaacaac aggtgttttc aaattagtgg
     4021 cagacgcgaa aacattgaaa gtgttagggg cgcatgtagt ggcagaaaac gcaggagacg
     4081 taatttatgc agcaacatta gctgtgaaat tcggtttaac tgttggagat ctgagagaaa
     4141 cgatggctcc atatctaaca atggcagaag gattgaagct ggctgtccta acttttgata
     4201 aagatgtttc gaaattatct tgctgtgcag gctaagggaa cttttttaca actccctatt
     4261 caagtgaacc agctaatgct ttaagggatt ttctaagtgc atttgtggtc acaaaaataa
     4321 tttaacactg atgatttcga catcaaatct ataattgata ccttaacacc gcagagtaat
     4381 aaaggatgaa taatatgtca gacttattat ccctaccaga cattaaaaca atagagccgc
     4441 cacaagaaaa tgaaaccgat atgatgttta aagttgaagc agtcggacca cctgaacgtt
     4501 gtcctgaatg tggttttgac aagttgtaca aacacagttc aagaaatcaa ctaattatgg
     4561 atttgcccat tcgtttaaag cgagtgggct tacaattgaa ccgtagacga tacaagtgtc
     4621 gtgaatgcgg atctaccttc tgggaacgcc taatatctgt agatgagaag cgtagtatga
     4681 ccaaaaggct tttaaagtcc attcaagagc aatccatgtc taagaccttt gtagaagtcg
     4741 cagaaagcgt tggtgttgac gagaaaacca ttaggaacgt ttttaaggac tatgtggcac
     4801 tcaaagaacg tgaataccag tttgaaactc ctaagtggct tgggatagac gagatacata
     4861 ttatccgtag acctcggctt gtattgacta atattgaacg caggactatt tatgacatca
     4921 agcctaaccg taacaaggaa acagtcatcc aacgtctttc agaaatcagt gacaggactt
     4981 acattgagta cgtcacaatg gatatgtgga agccctacaa agacgcagtg aaccctatcc
     5041 ttccacacgc taaagttgtc gtagataagt ttcatgtagt tagaatggct aatcaagcct
     5101 tagataacgt caggaagtct ttgaaagccc atatgagcca aaaagaaaga cgtaccctta
     5161 tgcgtgaaag gtttatcctt ctaaagcgta aacacgattt aaatgaacgt gaatcattcc
     5221 tcttagatac ttggttaggt aatcttcctg ctttaaaaga agcctatgaa ctcaaagaag
     5281 agttttactg gatatgggat actcctgatc cagatgaagg tcatcttcgt tatagtcaat
     5341 ggagacaccg ttgtatgtcc agcaactcta aagacgcata taaagacctc gtgagagccg
     5401 tagacaactg gcatgtcgaa atattcaact actttgataa aaggctcact aatgcttata
     5461 cggagtcaat taacagcatt attaggcagg tagagcgaat gggtagaggt tactcgtttg
     5521 atgccttacg agccaaaatc cttttcaatg agaagctcca taaaaagcgt aagccacgat
     5581 ttaattcaag tgctttcaat aaagctatgt tatacgatac tttcaattgg tatgaagtga
     5641 atgatcacga cattacagac aactttggtg tcgatttttc cacacctatt aagaatttgg
     5701 agaagggtga tttataagcc cttttccacc ataaaatccg aatacccaaa aaaacaaaaa
     5761 tatacagaat taggataata actaacaaaa acgaaaacaa gtataaaaga aatataatgt
     5821 ataggcactt gacatgtttt attttatcct gtattccaca aatttaattg cttataatga
     5881 tctactcttt gactgtattt atccggttct ctgaagattc ttactaacat atcggcttct
     5941 ttaaaaaatg tcttaatgcc ctgtttgtac gcagatttta cctgttgctt cacttgaatg
     6001 accgtacgaa ttaacgcttt acgagtggca actactagtc ccccattccg tccagctttt
     6061 atttgtacaa ataatttacc ttcggccatc aattctttta atgcacggtc aagagaacgt
     6121 ttaggaattg attgtccgtt gaaagtaatc gctgcttgca aatctgtttt cttcagtctg
     6181 acttcaggtc tgtatctata acattgggtt tcaaggtaag ctaaaatatc ttctttccat
     6241 tcggaaaggt gagatttctt tcttttatga cgttccttct taaatttgta ccaagtattt
     6301 ctttgttgtg taaacatttt ttcctcagac agcgattcta agccccaatt tgacactaac
     6361 tcctgtacat aagtcttact tgcacctcta tacttcccag agtaagcact ttttacaatt
     6421 cttactcgtt ctctatcttt tagaggacgt tctaaagaac tgttaaaaag atccatctca
     6481 tttagacagt cttctaagtc aactcctgat gcataattgg ccaaagataa agtgaaaatc
     6541 acattattcc ggcccataat ccctttagcg cctacaattt ttggattact aattaattgt
     6601 cgataccaca tttcgttcac ttgtttatat ttatttttaa caagtgaaag tagcggtttt
     6661 ttatatgttt tcgctggctc atatttcatt gaccattgga ttaattgctg tatatcatgt
     6721 gttaaagctg gataataata gacaatgttg tcagttctag ggatacgtgc tatcccaaaa
     6781 tgattgcaac ctaaatcgac tgaaggcaat tcttcagcaa atgcttttct taagttctca
     6841 gatactcgtt tggccacttc gattgactga taattatttt tggacgaaat ataccaggca
     6901 ttttcaagaa taaagtatgc ttggaagccc cctggtgttt ccaaaataag ggttggcatt
     6961 aattccagat cgattgctgc atcgagaatc atttgagaat ctaatttttc gcctgttaag
     7021 cgatcaaaat cgattacaaa agtattgatt tgttgcaagt tgcgttcgtt gtgccctttt
     7081 acaattattc ttttttcatc tgcatatgca ccatagctgt aaacgttagg tgtccaatga
     7141 gtaaatttat cttcattttc agctaagcct tcttgagaag taagaacgat tccacgagat
     7201 tccgtcatta acttcttgga tctaaaccca aaaatagcac ctttttttcc ttcagcttta
     7261 tagccgattg gtttaatttt gctgtttaaa cacttgtatt cacgaatacc actttttaaa
     7321 ataactgaaa tcacgtcaga aactttcatc ttatcaccac ccattctata tttaaaaaaa
     7381 ggagaactgc aggcattcta cagctctcct ttaaacctta tttgcagcta aaatatagct
     7441 caaaatcttg atttttaagg tcccaaatga tataattaaa aagactaaga gagctgcaac
     7501 tctctgtaat ctaaaactat aatgggacga aagagaatca tgaaatgctg tgtacatttt
     7561 gattctgtaa tttagttgat cggcaaatca actaagttac cgtctctatt tttttgtcta
     7621 taattcaatt agtaaatgta tcttttttct attcattcaa attcttcttt agaatacatg
     7681 tcactacaac ttttgtaaag taaaaatttt ctctcaaata atcattctta atttattttt
     7741 gtatcctttt atctttgtat ccttttatct ttttatcttt ttatcttttt atcctttaat
     7801 tttattgttg tatcttttta tctttgtatc cttttatctt tttatctttg tatccttatt
     7861 tagaagtcta tttaataact tttcttataa aatgctatct gattaaataa gtatgtttta
     7921 agataattag aaaaaagata catttatcta tttacaatta attctatttg tattactatt
     7981 taaatataga aaaaagatac aaagatacat ttatctttta ttacattttg attacaagga
     8041 aggagtatat tgatggctaa tacaattata tttggacaac aaaagggcgg ggtaggaaaa
     8101 acaactgaca cttgcatgat aacgttggta gctgcgatgc taatgaaaaa gcgtactatt
     8161 ttaattgata ttgatttgca agcaaatgca acaaatttta tgacaaaaac atttggggta
     8221 gaaacggtac ctcagacttt gatgtctgct cttgaaaatg gcgatctacg tcctgctatc
     8281 gtaaacctta aggaaaactt agatttaatt cccgctggtt atgatatgcg tctatacact
     8341 gaattcttaa cagaaaattt tgataataca atagatagaa ctttttatct taaaaacctt
     8401 attgaagaaa ttaaagacga ttatgattat atttttattg acatcccacc ttcaaccgat
     8461 ttaaaggtag ataatgctat ggttgcatgt gattacttgg ttgtagtaca agaaacacaa
     8521 caattttctt ttgagggaag tcaacggcta atttttgatt atatacaaac cttagttgac
     8581 gattttggct cagacgttcc aattcaaatt gcaggcattt tacctgcatt gttacaacaa
     8641 aaacgcccaa tgcaccaaaa attagttgag gaaacaatag acacatttgg aagagacaat
     8701 gtttttaaca ctattattca aaatcatgca cgtttagagt ggtatccacg gattggattt
     8761 caatttcaag actatcatga taaaaaaatg attgctcttt ttgcagatat tttctgtgaa
     8821 ttagaagaac gcatcactct ttataaaagt aatggtgata ttcaagatta tttttatact
     8881 ccgagatata tattagacgg aaaattaact tccctgggaa aggagattag tctaaatgtc
     8941 tagtttgata aatcgtaaaa tcccacaagg ggcaacagca aataaagaag cagatacaaa
     9001 acgaaaagtt actattcaaa aaaaatcgag taaaaagata ttagcttcag aaagaaaaaa
     9061 catcaaagtg tccccctcta cttttgactt aattaaaacc atatcaacaa tgaaaagcta
     9121 caaaaattat gaatttattg ataaagctgt agaaagctat atacaaaata atttaacaga
     9181 acgtgaacaa cgtatcctca aaaatttaat ctcaaaataa ataaaaaagg ataaaaagat
     9241 acatgtatct ttttatcctt ttttatttat tgatttcttt ttatcaatgc agattttacc
     9301 atttttatca agtagaggcg taattcctcc tttaggagtt tctaaataat tcacaccagt
     9361 aacagtatca actacaattt tatagcctaa tagagttaaa gattcacttt ctttttcaac
     9421 aaaacgtttg tctttaaata catgttcctt acatttatgt ctgtctaatg gtacgttatc
     9481 tgattgttct atcaactcac tatttgaatt atccccggca accatcatgc ctaaattttt
     9541 atcataataa atatattctc cacctagtat taaggttaat ggcataaaaa tcttcctttc
     9601 tttaattttc ttaccgtcct ttttctcttt ttttaacggc tttcgttact tacctttttg
     9661 ttcattttct acgcacaatc aatcgattaa acagattgcc tcttattcca ataagtaaat
     9721 ttcaagtgca aatcatccta tttttaaata ggatctgttg tcaacttttt cttaattcta
     9781 tcaaaaaacg gaagattttg cttgcaaacc aattcccttt tactaaatta aaataggttt
     9841 ttatttagta tgaaacagaa accaacattg aagtaaatct tatttgttta ttctttaatt
     9901 attgtaattg ctacatattc tccttgtttc tgtttgtatc ggcgatggca tttaggacag
     9961 taaaagtaat ttttttgata aacacttttt gctaattccc cacagtcaca ctccacgtga
    10021 aaagcaatat ttcgattaaa actagttatt tcaatgcccc aacgatcata agattttcgc
    10081 ttcataaaaa ttttcctcct tgtaaatttt actaacgatt ataaataccg ttgccattct
    10141 ttcaactgtt gactaattaa cgcataaaat tcgacttctg ataaaatggt aatctcatga
    10201 cctaacgcaa tcaagtcccg cgcggtttgt tccttttttg tatagagaga atcatcaaac
    10261 aacgacttag aatactgacc acaaaccagg aaatttgttt ttgaagtaac acttgattga
    10321 caatggccgt ttaaagagtg aaccaaaccg atggcttggg ttcgtgtcat ttgctgcagc
    10381 gttccagtaa aaacgaccct ttgattcgtt aagtccattt tcaaactctc ccttcccgtc
    10441 taatagctgt tatatctatc aagaaaagtg gtgcaaggta tgttcatatt ctttcacccg
    10501 tcgataaaag gtatttcgtt tcatgcccaa ttgatccata gcatagactg cagttatctt
    10561 tcctgctttc cagtcagaat agatcgcagg aaattcagga ggtaaagcaa ttttctttct
    10621 gccgaatttt acaccctttt gtttcgcagc ttctacgcct tctctttgcc ggctattaat
    10681 tcgtttccgc tcgtcttcgg ccatatagct gagtaattgc agaattaagt cagagataag
    10741 cgtctctaac ccatctagtt gcttgtaact agcagtatct aacagaggca tgtctaacac
    10801 agaaatatca atctgctttt ccttagttaa ttctcgccat tcttctaaaa tcatttgttt
    10861 atttctgcct agtctatcta atgattgtag atagagtaaa tctccttttc gaaacatccg
    10921 tttcattaat tgatattcag ggcgtttaaa atctttgccg ctggcttttt ctacaaatat
    10981 atattcttca gggattccta attccttcgc ggaaatcagt tgtcgttcga tcgattgatc
    11041 ataactactg acccggatat aaaaaaattt tctcatcgtt tttatctacc cttttttcac
    11101 taaaattgtt tcaaaacaat cagattaaat cagaaacgtt tcaaattgta ttatgtggtt
    11161 attatgccat acataaacaa agaaaatcaa ggcatgtcaa aaagtatact ttttgaaaca
    11221 gtaagtttac actcccgcat gattatattt attccgctaa aatacataaa aatggcaggc
    11281 cttaacggtc tgccattaaa taaactagtt atccagtttt tcttttatgt cttccatggt
    11341 atattttatt tttcacatca gaatttgact tctttaactt ttacaatttt tttaacacta
    11401 ttattctatt acaacactat atatttctaa tttagggtta aaaataaatc tttttgatac
    11461 ttttgccttt acaatataat tgtattcttt ctttttttct ttgaagtcgc ttgattcaac
    11521 tgtaacttca aaccacattt catttggaga ctcttttaat ttttttactt gaaatgaatt
    11581 atctcttgga tcataaggtg gtactctttc aaactcagga agttttctct gtatttgttc
    11641 ttcaggagta ttcgcaaagt aatctactat cttgacagtt attaaaagta ctactattcc
    11701 caaaactata acaaacataa agataattcc acatccagaa ttatttttat tattttgagt
    11761 cagattattc cattctattt cttccataat catcatgtcc ttttacaatt catatactta
    11821 ttttatcgta tatatttaaa aaggaaaagg acactgttta aatagtaaac gattcttaat
    11881 taagtttact tctattttct tgaaattgaa taattttttc agaaaatcca tttgcaactt
    11941 tcccctcatc tccaataaaa tcaagataag taaaatctgt cccaagtgaa ttattaacta
    12001 ttttatcaat gaaaaatgaa attatcccta cacctaagat atcaactgtt tcgtcatttt
    12061 ctaataaact atttgttact gaagcgattt tatctgaaga accaaatctg ttcttaacaa
    12121 attgattaaa tctatcaata gcacccttat cacaatagta cgtttttaaa gccgataaag
    12181 ctgttgaatc agaatttctt tttgctaagg tataaacatt taccgcgtca acatcttggt
    12241 ataaatcttc tagactaaat gttccacctg cagccgtacc tactaatgca aatacacctt
    12301 gctccatttg tattcctaat ttcttaactt caccccagca agtcattaaa tctcctaacc
    12361 aacctgcaaa gtcagaaaca ctatcattga ttgtgtcggc tagaaacatc tgtgattgta
    12421 acgtcacaat taagtgaggc aaacctatta ttgaaccagt attaggatct ataaaataat
    12481 tatacaggcc ctcgctatta gttaatttat tcctatattc atcataaatt tttctatcat
    12541 gctctgttac aggagaagaa atgatgtccc atgacaaacc agaataattt acatatctat
    12601 aaaatgtcaa atgagggttc caaggattat caagaccagc ttgtgataga tattgatacg
    12661 cacgtaccat ctgatttata accttctgat tgtatttggc aatgtaatta tcattattag
    12721 tatcatttga taactcatta aatccgctat ctctacccga tgtagcgatt aaatcgatac
    12781 caacagctcc gttgccactt cctatggtaa attcaacaaa ttgatcaaaa gaccattgtc
    12841 taggcatagg aaaacctaaa ttcccactat aaccagtcga catatctgaa acaaatgcaa
    12901 acgaagcata cccgttattg attatttgag aacaaacatt tcttgttccg taaacacccg
    12961 tttgataccc gctatatgac attacagcag ataccccctt aaaatattct ccagcatatg
    13021 caagaatttc ttcccctaac atgtctagat caacagcaaa ataaataact gttgttaatg
    13081 gtatatttag attttttgct gcatctatag ccttcttacc atctgataag ccacgatcat
    13141 aagtaaaata agctaatgct gcacctccat cttgatagat aggaaacaca gataaatttt
    13201 tagaaaaaat attattcaat tcctctgtag ttagatactt atttcgttca tcttttccaa
    13261 ttccaacagt accagtaaga tatctaccaa ctatttttat atcattatca accaatgttt
    13321 ggatttgaga tctagttaat tgcgtagaag tatctgcgcc atctgcagat cttctaatat
    13381 ctccagcgct agacaatagt ccttttatta caacaacatc tgctatttca gtatattcag
    13441 gcaataccat aaattttcgg aatgccagta cttctaaacc catatattta gtgaaaatac
    13501 catcgaatgt accgtcttca aaataaccat taacatataa tccatattga attattctta
    13561 catagtttcc cgagttacct tcagacacag gataagtttt actagttgtc ccattaccat
    13621 aagaccctgt tgcttcactg ggagaatatc ctagttcagc ttgtaatgca aaaattaatg
    13681 cttgatttga atttctttga taaataccat cacaaggcat tattcccgtg tattctaaat
    13741 aatttacatt taaccattgc tgcatttttc taattttact atctccgccg ggaacaagaa
    13801 caaaagcact catatcaaat aacgctttgg cccatttcgc atccaaaact ccagttacag
    13861 cgcttccatt aaaccctgca tcagtctgca actcttttac agcagtatct aaatatggag
    13921 tatatacccc atcgaagcca cctggattaa tccccttaca ccaaaaggca ccttgaataa
    13981 gaaatacaat atttttaggt agatttttgc cccatttagg agtaacctga ttatcgtaca
    14041 ttttttcagt ggtaggtcca aaattagcta ctagattagt aataccaagt tctacttgta
    14101 gtccttcaat aagtccatag attgtaggcc accctgttcg cccattttct ggaaccttat
    14161 caaaccctgt aacattactg tatttcttgt ttagccattt ctgagtactt aaaaccatct
    14221 catccataaa aatcccctgc tttcttgccg aacatacgtt cgtttatgtg ttgatt
//
LOCUS       DACCYH010000073        14183 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.076, whole genome shotgun sequence.
ACCESSION   DACCYH010000073 DACCYH010000000
VERSION     DACCYH010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.076"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..477
                     /locus_tag="IVY85_002110"
     CDS             <1..477
                     /locus_tag="IVY85_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8045118.1"
                     /translation="HLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETAL
                     NQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGIL
                     RRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            983..1585
                     /locus_tag="IVY85_002111"
     CDS             983..1585
                     /locus_tag="IVY85_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8045119.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1601..2278
                     /locus_tag="IVY85_002112"
                     /pseudo
     CDS             1601..2278
                     /locus_tag="IVY85_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2790..3086
                     /locus_tag="IVY85_002113"
     CDS             2790..3086
                     /locus_tag="IVY85_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HAP8045120.1"
                     /translation="MSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTNSASGVLRAN
                     EIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLKEKVVR"
     gene            3087..3503
                     /locus_tag="IVY85_002114"
     CDS             3087..3503
                     /locus_tag="IVY85_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP8045121.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3523..5067
                     /locus_tag="IVY85_002115"
     CDS             3523..5067
                     /locus_tag="IVY85_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8045122.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            5210..5434
                     /locus_tag="IVY85_002116"
     CDS             5210..5434
                     /locus_tag="IVY85_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8045123.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            5449..6318
                     /locus_tag="IVY85_002117"
     CDS             5449..6318
                     /locus_tag="IVY85_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP8045124.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            6299..7033
                     /locus_tag="IVY85_002118"
     CDS             6299..7033
                     /locus_tag="IVY85_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8045125.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            7066..7974
                     /locus_tag="IVY85_002119"
     CDS             7066..7974
                     /locus_tag="IVY85_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP8045126.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7971..8513
                     /gene="sat4"
                     /locus_tag="IVY85_002120"
     CDS             7971..8513
                     /gene="sat4"
                     /locus_tag="IVY85_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAP8045127.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8606..9400
                     /locus_tag="IVY85_002121"
                     /note="aph(3')-IIIa"
     CDS             8606..9400
                     /locus_tag="IVY85_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP8045128.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(9508..9696)
                     /locus_tag="IVY85_002122"
                     /pseudo
     CDS             complement(9508..9696)
                     /locus_tag="IVY85_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9676..10248
                     /locus_tag="IVY85_002123"
     CDS             9676..10248
                     /locus_tag="IVY85_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8045129.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
     gene            10588..11442
                     /locus_tag="IVY85_002124"
     CDS             10588..11442
                     /locus_tag="IVY85_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8045130.1"
                     /translation="MEKEKLKILEELRRILNNKNEAIIILNNYFKGGVGKSKLSTMFA
                     YLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKNGNLASSIIHLTDNL
                     DLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIIIDTVPTPSVYTNNAI
                     VASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFVPYLVDTDSATIKSN
                     LEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVLSMYENVFFEMIERI
                     IQLENEKE"
     gene            11541..11750
                     /locus_tag="IVY85_002125"
     CDS             11541..11750
                     /locus_tag="IVY85_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP8045131.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            11767..12039
                     /locus_tag="IVY85_002126"
     CDS             11767..12039
                     /locus_tag="IVY85_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8045132.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            12041..12904
                     /locus_tag="IVY85_002127"
     CDS             12041..12904
                     /locus_tag="IVY85_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP8045133.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(13167..13904)
                     /gene="erm(B)"
                     /locus_tag="IVY85_002128"
     CDS             complement(13167..13904)
                     /gene="erm(B)"
                     /locus_tag="IVY85_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP8045134.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(14029..14112)
                     /locus_tag="IVY85_002129"
     CDS             complement(14029..14112)
                     /locus_tag="IVY85_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP8045135.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT         5018 a         2222 c         2926 g         4017 t
ORIGIN      
        1 caccttgaag gtgatacgat cgttggcatt catcataaaa gtgccgtcat tactttagtt
       61 gaaagattat ctaaagtcat tatcacgatt aaacccaacg gccgtaaggc attagatatt
      121 gaaactgccc ttaatcaatg gttttctcgc ttccctaaaa acttctttaa atctattacg
      181 tttgactgtg gaaaagaatt ttctaactgg aaagccatta gtaaccaaca tgatattgat
      241 atatattttg cggaccctgg aacaccttct caacgcccat taaacgagaa ttctaacggg
      301 attctgcgtc gtaatggact gccgaaatca atggatttta gaaaagtgaa tcagacattt
      361 atttccagtg tcagcaatca acgtaatcat attccaagaa aatcattgaa ttacagaaca
      421 ccaatcgaaa tatttttgag ctatgtacaa gaagcatttt attctagctt aatttgacaa
      481 atcatactat gatacgtgtt tgtttttctg tgctgtttag tgaatgatta gcagaaatat
      541 agagagtaag attttaatta attattaggg ggagaaggag agagtagccc gaaaactttt
      601 agttggcttg gactgaacga agtgagggaa aggctactaa aacgtcgagg ggcagtgaga
      661 gcgaagcgaa cacttgatct tttaagttgc tatcttttta aggtcaatag agtatactta
      721 tttgtcctaa tatggtttta gcagtataat tgacttggtg aataggtcat ttaagttggg
      781 cataatagga ggagtaaaat gaaaaaattt atttatcgag ttttagaaaa tgacgaagtg
      841 gtggctattt ttaatgagca acaatatgcg caagatttta tcgcttacga aaagacaatt
      901 tctgataagc aatttgaaat tgaaaaagta gatatagctg attggttatt gcaaccgaga
      961 gaattttaga ggttggttga aaatggctaa aattggttat gcacgtgtca gtagcaaaga
     1021 acagaactta gatagacaac tgaaagcgtt agagggcgtt tctaaggtct tttcagacaa
     1081 attaagcggt caatcggtcg aacgcccaca attacaagct atgcttaact atattcgtga
     1141 aggggatata gttgttgtta ctgaattaga tcgattagga cgaaataata aagaattaac
     1201 agaattgatg aatcaaattc aaattaaggg ggcaaccctg gaagtcttaa atttaccctc
     1261 aatgaatggt attgaagatg aaaatttaag acggctgatt aataatttag tgattgaatt
     1321 gtataagtac caagcggaat ctgaacgcaa acgaattaaa gaacgccaag cccaaggaat
     1381 tgaaattgct aagaaaaaag gaaaattcaa agggcgacaa cctaaattca aagaaaatga
     1441 tccacgttta caacacgctt tcgatttatt tttgaatggt tgttccgata aagaagttga
     1501 agaacaaact ggaattaatc gccgaacgtt tagaaggtat cgatcaagat acaacgtgac
     1561 agtagatcaa agaaaaaaac aatgaaaaga gggatagtta atgagtacgg ttattttagc
     1621 tgaaaaacca agccaggcat tagcctacgc aagtgcttta aaacaaagca ccaaaaaaga
     1681 cggttatttt gagatcaaag acccactatt tacagatgaa acgtttatca cctttggttt
     1741 tgggcattta gtcgaattag cagaaccagg tcattataac gaaaagtggc agaactggaa
     1801 acttgaatct ttgccgattt ttcctgatcg atacgatttt gaagttgcaa aagataaggg
     1861 aaagcagttt aaaattgttg ctgaacttct caaaaaggca aatacaatta ttgtcgcaac
     1921 agatagcgac agagaaggtg aaaatatcgc ctggtcgatt atccataaag caaatgcctt
     1981 ttctaaagat aaaacgtata aaagattatg gatcaatagc ttagaaaaag atgtgatccg
     2041 aagcggtttt caaaatttgc aaccaggaat gaattactat cccttttatc aagaagcgca
     2101 aacacgccaa attgccgatt ggttgatcgg catgaacgca agccctttat ataccttgaa
     2161 tttacaacaa aaaggcgtac aaggtacatt ttcattagga cgtgtacaaa caccgacttt
     2221 atatctcatt tatcaacgcc aggaagcaat agaaaacttt aaaaaagaac cttttttcct
     2281 gaataattca tagttttact aatagtcttt cctaacattg atttaatagt ctttaaagta
     2341 ttctgcttca tcacccatta ggtgatgaaa aaagaacccc tttctgattt agcggacaaa
     2401 tactttgacc taatctcaga agatataaaa gaaaaaataa taaaatatcc gatttatact
     2461 ttgtataaag aactaaagta gttagcaaat catttacagc aaatcactat gttccacata
     2521 ccattttaca tacttacctt atccacgaca catgttgaga tagtatcgaa gataaagaaa
     2581 gttgctccat gttgtttttg cgagatgggg aaatcaaagc cacattgaca accatgatga
     2641 ataagcttgc tttcagtcat aagctaatcc tagaaccact tttcaagtca gttagccaaa
     2701 tcgatgaaga aagcgaccgt gaaagaatgg acgctattga taagctaatg gagcaactat
     2761 tggaagaacg caataccctt attgcactga tgtccaaagg tttcctagaa cctgctcttt
     2821 ttaatcagga acgaaatgtt ttagatagtg agattaaaaa tcttactacc gaaaaaacaa
     2881 acctggtaac gaactctgcg agtggggttt tgcgagcaaa cgagataaag gacctcatta
     2941 attatgtgtc agcagataat tttaatggtg actacacgga agaactattt gaagaatttg
     3001 tagtgaacat cattgtaaat tccagggatg agctgacatt caatttgaaa tgcggtcttt
     3061 ccctgaaaga aaaggtggtg agataaatgg catatattcc atatggatac aaaattcaag
     3121 atggagtggt tactgtcgat gaaaaggcag caggtcaagt aaaggtattc tttgagaaat
     3181 acatatcagg actatccctt acagtggctg gcgaacaggc aggtattgaa aagacacatt
     3241 ctgtgatggg gcgcattttg aaaaacgtca actaccttgg aaatgatacg tatccagcaa
     3301 ttattgataa agagatattt gataaagctg aagaagttag agataaacgt gcaaaggatt
     3361 taggacgagt ggtagagctt gccgctttca cctctccccc tcccaaagaa cgattcaaaa
     3421 tgaaaaaggc agataataag atgccagtgg atccttttga acgagcagaa tacttataca
     3481 gtctgataga aagcgaggaa taaagtgaca gagaaaaata taatggttat tcctgctcgt
     3541 aaaagagtag gaagtacagc cgcaaaagaa aagataaaga aacttcgtgt tgctgcctat
     3601 tgccgtgttt ctacagaaac agaagaacaa aattctagct atgaggttca ggtctcacac
     3661 tatacagagt ttataaagaa aaataatgaa tgggagtttg ctggcatctt tgcagatgat
     3721 ggtatctctg gtacgaacac taaaaagcgt gacgaattta atcgcatgat tgcagagtgt
     3781 atggacggta acatcgacat ggttattaca aaatccatca gccgatttgc gcgtaacacc
     3841 ctagactgcc ttcaatacat tagacagctc aaggataaga acatatcagt ttattttgag
     3901 aaagagaaca tcaacaccac ggatgccaaa ggtgaggttt tgctgactat tatggcatct
     3961 ttggcacagc aggaaagcca gagcctttca caaaacgtta agcttgggct acagtaccga
     4021 taccaacaag gaaaggtgca ggtcaaccat aagcgattta tgggctacac gaaagatgaa
     4081 gatggaaatt taactattgt ccccgaagaa gctgagatta tcaaacgcat ctaccgagaa
     4141 taccttgagg gtcagagtct agtgggcatt ggtcgagccc ttgaaaagga tggtatttta
     4201 acagcagcgg gaaaaccaag atggcgacca gaatcagtta agaagatact tcaaaacgaa
     4261 aaatacatcg gagatgccct tctgcaaaag actgtcacag tagattttct gaccaagaaa
     4321 cgagtgaaaa atgaagggca tgttccccaa tattatgttg aaaatagcca tgaagcgatt
     4381 attcctaaag agttattttt gcaggcgcaa gaagaaattc atcggagaag caatatctac
     4441 acaggagaag gcaagaacaa aagaatttac agtagcaagt acgctttaag tgccatcacc
     4501 ttctgtggag attgtggcga tatttataga cgggtctatt ggaatatcca cggaagaaaa
     4561 gaatttgtct ggcgatgcgt gactagaatc gagcaaggtc ctgaagtttg taagaaccga
     4621 accgtaaaag aagatgaact ttatggtgca gtaatgactg cgattaataa gctacttgca
     4681 ggtgggaaca atatgataaa gacactggaa gagaatattc atgctgtgat tggcgaaacc
     4741 acagaatacc aaatttcaga gattaacaac tcactagagg aaaaacaaaa agaactaatc
     4801 aagctggcga acaagggtca agactatgac cacttagcag atgagattga tgagctgaga
     4861 gacaagcgac agatcctttt agtagaagat gcctccctca gtggcgagaa cgagcgaatc
     4921 aatgagctga ttgaatttat ccgcaagaac aaattccgca ccttagaata tgatgataag
     4981 cttgtaagga agataatcca gagcgttaca gtctatgaag gccacttcgt catagccttc
     5041 aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcccatg actctacagt
     5101 agagttgtgg gttttctttt gcttgctgat tataataata ttttaataac tattgtgttt
     5161 atcaaccaaa tggtttataa tattcttagc gtaaacttgg aggtgctgta tgactacggc
     5221 agaaatgatt aaagaactgt gtgagcaaat gaatataagt gtttccgaac ttgctagacg
     5281 tattggccag actccacaga atttcaataa aaaattacaa cgagaaacag taaccttgga
     5341 tgagttgaag gccattgctg atgtgctagg tgtcaagttt gtgcaggcat ttattttacc
     5401 agatggagaa gaaataaaaa tatctaacga ataaaggagg atgtttatat ggtaggtaat
     5461 gttattcaaa tagtaacaga gaaattatca tctttgcctt ttatagaagg catcgtatta
     5521 gggggctcac gtgcaagagg cacccataca gaaaattctg atatagatat cggaatctat
     5581 tacaattcag actcatttga cctgacagcg attaatcaaa ttgctacaga gttggatgat
     5641 gagaatagaa acaaccttgt tgtacctccc ggagcgtggg gtgattgggt taatggcggt
     5701 ggatggttag ttataaacgg gtgtcatgtt gacttgatat tacgtgatat aaaacgggtg
     5761 gaacaaataa tcaaagatac ggagcaagga attgttactg ccaattatca gaccgggcat
     5821 ccccatggtt atataagtgc tatgtatcgt ggagaattag cgattagtaa aatactatat
     5881 gctaagaacg aaagcttatg cgaattaaaa aagcaggcag aaatttaccc tactgctcta
     5941 aagaaaagct tgatgaactt ttttatattt gaagctgaat tctctttaat gtttgtaaaa
     6001 gcaaatgcgg gggcagagga taaatattat attgcaggcc atgtttttcg tattatttca
     6061 tgcttaaatc aagtactatt tgcatgtaat aatgcttatt gcattaacga aaagaaagct
     6121 ataaaactgc ttgaaacttt tgaatataaa cctgaaaaat atgccgaaag agttaatcat
     6181 atttttgaag tactcggtct ttctcttttt gaatgctacg acatgaccga gaaactttat
     6241 aaagaagtga atgaaattgt atcggagata aataactttt taaacgagga gagttcagat
     6301 gaaagaaaac aaatatgatg ataatatatt ttttcaaaaa tacagtcaaa tgagtcgctc
     6361 gcagaaagga ctggctggtg cgggagaatg ggagactttg aaaaagatgc tacctgattt
     6421 taagggtaag cgtgtgcttg atttaggatg cggctatgga tggcactgta tatatgcgat
     6481 ggaaaacggt gcttcctctg tagtaggtgt tgatatttct cataaaatgc tcgaagtagc
     6541 aaaaggaaaa acccattttc cacagattga atatgaatgc tgtgccatag aagatgtgga
     6601 tttcccagag gagagctttg atgtaatact aagttcgctt gcgtttcatt atgtagcaga
     6661 ctatgagaat ttaataaaaa agatatatag gatgctgaag gctggtggca atttagtttt
     6721 tacagttgaa catcctgttt ttactgctca tggaacacaa gactggtatt ataacgaaaa
     6781 aggagaaata ctgcatttcc cggtggacaa ttattattat gagggcaaac ggacagctat
     6841 gtttttggaa gaaaaggtta caaaatatca tagaacactg accacatatc taaatacact
     6901 gctttcaaat agttttataa taaatcagat tgtggagcca cagccgccag agaacatgat
     6961 ggatattccg gggatggcgg atgaaatgcg acgcccaatg atgctgattg tatcggcaaa
     7021 aaagaagatg taataatata gaaaaaataa acgaggagta tgtaaatgag atcagaaaaa
     7081 gaaatgatgg atttagtact ttctttagca gaacaggatg aacgtattcg aattgtgacc
     7141 cttgaggggt cacgcgcaaa tattaatata cctaaagatg aatttcagga ttatgatatt
     7201 acatattttg taagtgatat agaaccgttt atatctaatg atgactggct taatcaattt
     7261 gggaatataa taatgatgca aaagccggag gatatggaat tattcccacc tgaagaaaag
     7321 ggattttcct atcttatgct atttgatgat tacaataaaa ttgatcttac cttattgccc
     7381 ttggaagagt tagataatta cctaaagggc gataaattaa taaaggttct aattgataaa
     7441 gattgtagaa ttaaaaggga catagttccg actgatatag attatcatgt aagaaagcca
     7501 agcgcaaggg agtatgatga ttgctgcaat gaattttgga atgtaacacc ttatgttatt
     7561 aaaggattgt gccgtaagga aattttattt gctattgatc attttaatca gattgttcgc
     7621 catgagctgc tgagaatgat atcatggaag gtcggcatcg aaacaggctt taaattaagt
     7681 gtaggcaaga actataagtt tattgaaagg tatatatccg aggatttgtg ggagaaactt
     7741 ttgtccacct accggatgga ttcctatgaa aacatatggg aagcattatt tctatgccat
     7801 caattgttca gggcggtatc cggtgaggtg gcggaaaggc ttcattatgc ctatccggag
     7861 tatgatagga atataacaaa atataccagg gacatgtata aaaaatacac tggtaaaacc
     7921 ggctgcctgg atagcacata tgccgctgat atagaagaga ggcgggaaca gtgattacag
     7981 aaatgaaagc agggcacctg aaagatatcg ataaacccag cgaaccattt gaggtgatag
     8041 gtaagattat accgaggtat gaaaacgaga attggacctt tacagaatta ctctatgaag
     8101 cgccatattt aaaaagctac caagacgaag aggatgaaga ggatgaggag gcagattgcc
     8161 ttgaatatat tgacaatact gataagataa tatatcttta ctaccaagac gataaatgcg
     8221 tcggaaaagt taaactgcga aaaaattgga accggtacgc ttatatagaa gatatcgccg
     8281 tatgtaagga tttcaggggg caaggcatag gcagcgcgct tatcaatata tctatagaat
     8341 gggcaaagca taaaaacttg catggactaa tgcttgaaac ccaggacaat aaccttatag
     8401 cttgtaaatt ctatcataat tgtggtttca aaatcggctc cgtcgatact atgttatacg
     8461 ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt tagaatgcaa
     8521 ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc tttaaatact
     8581 gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg aattgaaaaa
     8641 actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg ctaaggtata
     8701 taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc ggtataaagg
     8761 gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag gaaagctgcc
     8821 tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc tcatgagtga
     8881 ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg aaaagattat
     8941 cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg attgtcccta
     9001 tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata acgatctggc
     9061 cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc gcgagctgta
     9121 tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg gcgacctggg
     9181 agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc ttgggagaag
     9241 cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca gggaggatat
     9301 cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc ctgattggga
     9361 gaaaataaaa tattatattt tactggatga attgttttag tacctagatt tagatgtcta
     9421 aaaagcttta actacaagct ttttagacat ctaatctttt ctgaagtaca tccgcaactg
     9481 tccatactct gatgttttat atcttttcta aaagttcgct agatagagtt ctatattaga
     9541 gatgtaaaga gttttggtga agaggtttgt gagtatggct tttataacca gggtgcaatg
     9601 agaagcacta acagcagcta gcttaagaac gcataggata tactttttat ggaagtcaca
     9661 gaggaggaat aaaatttgaa caggattaac agagtaacct ctattcttat tcagctgcaa
     9721 tcaaaaaaaa taattcctgc caaagaaatt gcacagcgat ttaatataag cttaaggaca
     9781 gtttacagag atatccggac acttgaagaa gccggaatac caattggatc tgaggccgga
     9841 aaaggatatt ttcttgtaga gggcttccta cttccgccgg taatgtttac agcggcggaa
     9901 gtgggtgcat tgattacagc agggaaattt ttaaattgcc atggagatga atcatttata
     9961 aaggattttg attcagctat gtataaaatc aaatctattt tgaagcatgg tgaaaaaaac
    10021 tatgcacagg agttggagaa cagtattaac gtgtacagca catctggaca gaaaaataca
    10081 ttggcggata acgttattgc agcaattcag actgcaatct gcaataaaag ggtaatatca
    10141 attcaatatc ctgcatcagg agggcaagaa ccggaaagca gaatgataga gcctatatca
    10201 ctggggtttt atgaacagaa ctggtactta atcggttttg caggctaaga aatgaatata
    10261 ggaattttcg tgtggatcgg attaaaagca tttgcataga agaagagctc tgtcaaagcc
    10321 atgatggctc tttggaacaa atattaaaac aaatgctttc atataaaaaa ctatatgccc
    10381 tacaatgccg ttgtaaaaat tggggaaaag ggatatattg attttatatc tttctcaaaa
    10441 taaatataaa agccctttaa agagggcttt tatatattaa tcacaaatca cttatcacaa
    10501 atcacaagtg attaatcaca aatcacttgt gatttgtgat tcttaatgat acaatattac
    10561 tatacaaaaa aagaatgggg cgtagttatg gagaaggaaa aactaaaaat acttgaagaa
    10621 ttaagacgta ttttaaacaa taaaaatgaa gcaattatta tcttgaataa ttactttaaa
    10681 ggtggtgttg gaaagtccaa attatcgact atgtttgctt acttgacaga caaattgaat
    10741 ttaaaagttt taatgatcga taaggactta caggcaacat tgacaaaaga cttagcaaaa
    10801 acttttgagg tagaattgcc acgtgtcaat ttttatgaag gcttgaaaaa tggaaacttg
    10861 gcttcttcta ttattcattt gactgataat ttagacttga tccctggcac gtttgatttg
    10921 atgttactgc caaaattaac tcgctcatgg acgtttgaaa atgaaagtag attgcttgct
    10981 actcttttag cacctttaaa aagtgactat gatcttatta ttattgatac tgtaccaacg
    11041 ccaagcgttt atacaaataa tgcaatcgtg gcgagtgatt acgttatgat ccctttacaa
    11101 gcagaagaag aaagtacaaa caacattcaa aactatattt cctatttgat tgatttgcaa
    11161 gaacagttta accctggact agatatgatt ggttttgttc cttatttagt tgatacggac
    11221 agcgcaacga taaaatcaaa cctggaagaa ctgtacaaag aacataaaga ggataatttg
    11281 gttttccgaa atattatcaa gcgaagtaat aaagtaagta cttggtctaa aaatggcatt
    11341 acagaacaca aaggctatga caaaaaagtt ttgtctatgt atgagaacgt attttttgaa
    11401 atgattgagc gaatcattca attagaaaat gaaaaagaat agaatcacaa atcacaagtg
    11461 attaatcaca aatcacttgt gatttgtgat aggtgatgat aaaataaata gtaagaagaa
    11521 atagaaagaa gtgagcgatc gtgggaaatt taggcgcaca gaaagcaaaa cgaaatgata
    11581 cgccaatcag cgcaaaaaaa gatataatgg gggataagac ggttcgtgtt cgtgccgact
    11641 tgcaccatat cataaaaatc gaaacagcaa agaatggcgg aaacgtaaaa gaagttatgg
    11701 atcaagcctt agaagaatat atacggaaat atttacctga caaactttaa aaggagtgaa
    11761 aatgaaatgg cagttacgta tgaaaaaaca tttgaaattg aaatcattaa tgagttatcg
    11821 gcaagtgttt ataatcgggt attaaattat gttttgaatc atgaactaga tactaaaaat
    11881 actcgtttac tagaagtgaa tcttttaaat caattagaag tggcacaaga agttgattta
    11941 tttcaacaac catttgaaga attacaagct attcatgagt attggcggtc aatgaatcaa
    12001 tattcaaaac aaattttgaa taaagagaaa gtggcttaac atggcgaata ttactgactt
    12061 caccgaaaag caatttgaag atcgtttaga aaagaatgtt gaacgactaa ctaaaaatag
    12121 actagcggtt gaatcgccaa ccgctttttt acttggtggg caaccagggt cagggaaaac
    12181 cagtttgcga tcagcaattt ttgaagaaac acaagggaat gttattgtca ttgataatga
    12241 tacctttaaa caacagcacc caaattttga cgaattagtg aaactctatg aaaaagacgt
    12301 agtaaaacac gttacccctt attcgaatcg catgacagaa gcacttatta gccgtttaag
    12361 cgatcaaggc tataatttag tgattgaagg aacaggacga acaacagacg ttcccattca
    12421 aacagccaca atgcttcaat ctaaaggtta tgaaacaaaa atatacgtca tggcagtacc
    12481 gaaaattgaa tcatacttag gaacgattga acgatatgaa accatgtatg cagatgatcc
    12541 aatgacagct agggcaacac caaaacaagc gcatgatatt gttgtcaaaa acttaccgac
    12601 caatttagaa acccttcata aaacgggctt atttagcgat ataaggcttt acaatagaga
    12661 aggagtgaaa ctctattcaa gcttagaaac gccttccatt agtccgaaag agaccttaga
    12721 aagagaatta aatcgtaaag tatcagggaa agaaattcaa ccgactttgg agagaataga
    12781 gcaaaaaatg gttcaaaatc aacaccaaga aacccctgaa tttaaagcaa ttcaacaaaa
    12841 aatggaaagc ttgcagctac ctacaccacc aatacccaaa acacctaaac ttccaggtat
    12901 ttaaacctta aaaaaggaaa agagtagtta ccaaaaaacg gtaactactc tttttttata
    12961 aaaacatttc ctcatgttgg ggtcccgagc gcctacgagg aatttgtatc gataagaaat
    13021 agatttaaaa atttcgctgt ttttttctaa tttcattttg aggtgaatta attggtagtt
    13081 attagaggtg ccctatacct aataatttat ctacattccc tttagtaacg tgtaactttc
    13141 caaatttaca aaagcgactc atagaattat ttcctcccgt taaataatag ataactatta
    13201 aaaatagaca atacttgctc ataagtaacg gtacttaaat tgtttacttt ggcgtgtttc
    13261 attgcttgat gaaactgatt tttagtaaac agttgacgat attctcgatt gacccatttt
    13321 gaaacaaagt acgtatatag cttccaatat ttatctggaa catctgtggt atggcgggta
    13381 agttttatta agacactgtt tacttttggt ttaggatgaa agcattccgc tggcagctta
    13441 agcaattgct gaatcgagac ttgagtgtgc aagagcaacc ctagtgttcg gtgaatatcc
    13501 aaggtacgct tgtagaatcc ttcttcaaca atcagataga tgtcagacgc atggctttca
    13561 aaaaccactt ttttaataat ttgtgtgctt aaatggtaag gaatattccc aacaatttta
    13621 tacctctgtt tgttagggaa ttgaaactgt agaatatctt ggtgaattaa agtgacacga
    13681 gtgttcagtt ttaatttttc tgacgataag ttgaatagat gactgtctaa ttcaatagac
    13741 gttacctgtt tacttatttt agccagtttc gtcgttaaat gccctttacc tgttccaatt
    13801 tcgtaaacgg tatcggtttc ttttaaattc aattgtttta ttatttggtt gagtactttt
    13861 tcactcgtta aaaagttttg agaatatttt atatttttgt tcatgtaatc actcctgaag
    13921 tgattacatc tataaataaa tacagaagtt aaacgatttg tttgtaattt tagttatctg
    13981 tttaaaaagt cataagatta gtcactggta ggaattaatc taacgtattt atttatctgc
    14041 gtaatcactg tttttagtct gtttcaaaac ggtagatgtt ttatctacat tacgcatttg
    14101 gaataccaac atgacgaatc cctccttctt aattacaaat ttttagcatc taatttaact
    14161 tcaattccta ttatacaaaa ttt
//
LOCUS       DACCYH010000074        13959 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.077, whole genome shotgun sequence.
ACCESSION   DACCYH010000074 DACCYH010000000
VERSION     DACCYH010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.077"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..135
                     /locus_tag="IVY85_002130"
     CDS             <1..135
                     /locus_tag="IVY85_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8045136.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(313..2310)
                     /gene="tkt"
                     /locus_tag="IVY85_002131"
     CDS             complement(313..2310)
                     /gene="tkt"
                     /locus_tag="IVY85_002131"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP8045137.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2412..2663)
                     /locus_tag="IVY85_002132"
     CDS             complement(2412..2663)
                     /locus_tag="IVY85_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP8045138.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2810..3433
                     /gene="lexA"
                     /locus_tag="IVY85_002133"
     CDS             2810..3433
                     /gene="lexA"
                     /locus_tag="IVY85_002133"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP8045139.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3613..3831
                     /locus_tag="IVY85_002134"
     CDS             3613..3831
                     /locus_tag="IVY85_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045140.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4120..4632)
                     /locus_tag="IVY85_002135"
     CDS             complement(4120..4632)
                     /locus_tag="IVY85_002135"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP8045141.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="IVY85_002136"
     CDS             complement(4646..6943)
                     /gene="recJ"
                     /locus_tag="IVY85_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP8045142.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7085..7519)
                     /locus_tag="IVY85_002137"
     CDS             complement(7085..7519)
                     /locus_tag="IVY85_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP8045143.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7529..8317)
                     /locus_tag="IVY85_002138"
     CDS             complement(7529..8317)
                     /locus_tag="IVY85_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8045144.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8314..9255)
                     /locus_tag="IVY85_002139"
     CDS             complement(8314..9255)
                     /locus_tag="IVY85_002139"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP8045145.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9432..10019)
                     /locus_tag="IVY85_002140"
     CDS             complement(9432..10019)
                     /locus_tag="IVY85_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP8045146.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10240..11553)
                     /gene="obgE"
                     /locus_tag="IVY85_002141"
     CDS             complement(10240..11553)
                     /gene="obgE"
                     /locus_tag="IVY85_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP8045147.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11841..12851
                     /gene="gap"
                     /locus_tag="IVY85_002142"
     CDS             11841..12851
                     /gene="gap"
                     /locus_tag="IVY85_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8045148.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12936..13871)
                     /locus_tag="IVY85_002143"
     CDS             complement(12936..13871)
                     /locus_tag="IVY85_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8045149.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3863 a         3126 c         2454 g         4516 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat
      181 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
      241 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      301 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      361 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      421 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      481 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      541 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      601 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      661 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      721 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      781 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      841 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      901 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      961 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
     1021 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
     1081 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
     1141 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1201 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1261 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1321 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1381 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1441 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1501 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1561 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1621 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1681 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1741 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1801 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1861 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1921 tgccataccg acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1981 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     2041 caaatcatcg attgtcactt gatagccagc taaatgaagc aagctataca acattgctga
     2101 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     2161 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2221 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2281 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2341 ttacctttat ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc
     2401 agtatttttt actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct
     2461 tttaattttt ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata
     2521 tgatgacgca taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct
     2581 tgttcttcgg cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc
     2641 tctatttttt caggtgacag catgctcagc cctcctttta ctctccttca tattatataa
     2701 aaactgaaaa aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc
     2761 gctttttata tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca
     2821 aactgaatca agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg
     2881 atacccgccg actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt
     2941 tcacggtcat cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc
     3001 tcgtgcaatc gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat
     3061 tccagtactt ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga
     3121 cttctttcca atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat
     3181 tcgtggagat agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa
     3241 gcaagaaact gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac
     3301 ttgcaaacga ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct
     3361 cgaaccaatc atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag
     3421 tcatatctat taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg
     3481 gtctcaaact cttttttcgc ccgggttctt cttctttcaa atttttatca cttccctctt
     3541 tacaaaccga acaaatattc gtatactaaa tatacgaaca attgttcgca taaattaaag
     3601 aggtgagaat tcatgaaatc aatgagacgt cttggcttag tattaactgt tttgttttta
     3661 ggtattatta ttggtaattt cggcatacgt ccagctttac ttgtgtttgt tccgttattt
     3721 actttgtggt tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg gcgcagaatt
     3781 catgaagatc atttctatca tgagccctac tacagatcca ggcaatcata ataaggattg
     3841 actgctaatt attcaaaaga caagaagtta aacttttacg ggggtgtgac aaaaatcggt
     3901 cacatccttt ttttcgaata aacggtgttc taaagccagt ttttcggtat taagtcaaat
     3961 tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat ttataaaaaa
     4021 atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt taaaatcgct
     4081 tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca ttaatgtaat
     4141 gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga tcaaaaatgc
     4201 gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt tgattgttcc
     4261 tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa tggcatcctt
     4321 atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga tggtttctcg
     4381 aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat aagcaacagg
     4441 acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc gtttttcttt
     4501 tgcataattt acgatttgtt tagtagcttc ccgataagcg tcaccatctg ccatcagtgg
     4561 ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg caatataatc
     4621 ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg gtaggaatat
     4681 cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgtcgctgg tataatctgc
     4741 tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg ttctctattg
     4801 taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt tcagggatac
     4861 gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct tgagaatgga
     4921 tcaatttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa taggcctcat
     4981 caggtgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa acttctttca
     5041 gatccgctaa atcatctgga caatccataa caactagaga gtttggtttt cttttttcaa
     5101 ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg ataatctcag
     5161 gcacgaattt caacgtcgaa gaatcaaatg ctaaataaag gctaccttcc gacgttcggc
     5221 tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc acagaaaaat
     5281 cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa agctttccta
     5341 ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca aaagcgaccg
     5401 catcaagctg actcgatgca tcggaaagga caaatttcag atgctgttgg tttgctccga
     5461 ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa ggattatccg
     5521 taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact tcatttggca
     5581 gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga tgatcgacta
     5641 tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc gttaaaccga
     5701 ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca agcatttcaa
     5761 aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct tcttttaaag
     5821 taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca atgcccaaaa
     5881 cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta ttgaccatcg
     5941 ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg atctcattca
     6001 atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac gagacggcag
     6061 gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa aaaccgatcg
     6121 ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc tgaagtccta
     6181 tccgttctga atgatgaatg gcatcgattc ctaatgaaac aaggattcga ttctcatcag
     6241 tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat tctgcaggcg
     6301 gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc aaatctggaa
     6361 atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct ggtaaattct
     6421 caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga gcgtatgcta
     6481 ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt ccttcttcga
     6541 tcttttcttg gtataccgat tggtttggtc catacccatg ctcaaatcga tttggcagga
     6601 atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt gcactggtga
     6661 tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt acagcttctt
     6721 ggattcggct gattgcttta tccatatcgt gtatcagata cggatcatgt aattcttgtt
     6781 cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt tgccacagta
     6841 acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag gattcagaag
     6901 gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca ctcctaactt
     6961 agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa aaacagatca
     7021 gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt atgaaatatc
     7081 cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt tcttccggcg
     7141 ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa gcagcttcca
     7201 actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct tcaattttct
     7261 tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt tgcttttttt
     7321 gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc agcgcagaac
     7381 cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt acaggaacat
     7441 ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat ccaagaatca
     7501 cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg ctggctaaat
     7561 aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa ataaacggtt
     7621 gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc aacttggcag
     7681 gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca gccttatcga
     7741 agaaatttgt ctcgattgga cctggattca ctgtcgtaac agctacgcca agtggtttca
     7801 attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca gaataaaccg
     7861 ttgattttgc agttgccatt tttccagcca tagaggcgat attaatgata tgaccttgtc
     7921 ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct aagatgttga
     7981 cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg aaaatcccaa
     8041 aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc ttttcatata
     8101 attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc tctttgccgc
     8161 tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt gcgcaactta
     8221 taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga ccgcctgaac
     8281 tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct cgcgaaatgg
     8341 aatctcaatg ctatcaaaat ctttcataat cgtcgtgttt tcaaacactt cttgtgcttc
     8401 tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag taagtagcag
     8461 ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg aatggaaata
     8521 gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca gaatatcgct
     8581 attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa tagtcacgat
     8641 tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc catcttctag
     8701 ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa tacctaattc
     8761 ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac gataaccaaa
     8821 actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat cagtaaaaat
     8881 cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg tttgcgaaat
     8941 acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct ctaatggcgt
     9001 atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa tatgatcgcc
     9061 gtgtaaatgt gtaatgaaaa ttttaccgat ttttctcgga cgaatagtcg ttcgcaaaat
     9121 ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct cgtctaaaag
     9181 tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac ctgttcctaa
     9241 aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt ttagggtaaa
     9301 aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa cgttctaaag
     9361 cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt gagatcctct
     9421 gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt ttgcggatgt
     9481 ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc tttcgcatca
     9541 tgcacatctt tttcgacatc ttcataatcc aagcgagtaa tttctccgcc taattccatc
     9601 attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat cagtcctgta
     9661 gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc ttttatttct
     9721 ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa aatccctagc
     9781 ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga tttgttatta
     9841 ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg atcatggctg
     9901 acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt gatttctgaa
     9961 aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg tttgctcatt
    10021 aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgaa aaataagaaa
    10081 agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta tcacagctcc
    10141 tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct cccttaaatc
    10201 tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa attcaaattc
    10261 aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa gagcttcatc
    10321 cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat gatcgaaatt
    10381 cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg catctgaatc
    10441 acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag tttcttcttc
    10501 cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg ctgtagcatt
    10561 caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg ggatgtcatc
    10621 agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta gattttccgg
    10681 tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta gcaagcgtaa
    10741 gttataagta gataattctt tgttgatggc aagatagtct tcataaggat ctcgtccttc
    10801 catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat gacgaaggaa
    10861 ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca agtctgctgc
    10921 ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttaggtacga gcgtcgtgaa
    10981 atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag tcgatttccc
    11041 gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta gttcgatttt
    11101 tcgctcttgt cctggttcac cgttttcagc aatttccggt gcaggattcc gaggagaggc
    11161 aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg tttgaccatt
    11221 ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg ttccttgtgg
    11281 tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca tcccattttc
    11341 acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac gtaacccttc
    11401 atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg gtccgccgtc
    11461 aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc cagctttcac
    11521 atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt attcatttat
    11581 ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta tcggtgaaat
    11641 ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt ttatcatttg
    11701 gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa tattctttgc
    11761 gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac agcaccccta
    11821 ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt gggcgtattg
    11881 gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta gtagcaatca
    11941 acgacttaac aagtccaact atgttagcac aattattgca atttgactct actcatggta
    12001 cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc gaagcaactc
    12061 gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac ggtgtagata
    12121 tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg catttagatg
    12181 ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc atcgtctata
    12241 acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt tcatgtacaa
    12301 caaactgttt agcacctatg gcttatttct taaataatga atttgggatc gaagttggta
    12361 caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga cctgtaaaag
    12421 gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat tctactggtg
    12481 ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa ggacacgcac
    12541 aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg aaaacaaaag
    12601 taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat ccttcattcg
    12661 ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa ggttcgatct
    12721 ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt aaaactgttg
    12781 catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta gaaaaattcg
    12841 ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat ccagtcaaag
    12901 gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg gcttaaaaat
    12961 tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc atgctcggct
    13021 ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt atctaacatt
    13081 tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg ctggtttttc
    13141 ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac ttttgtcact
    13201 agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc tagctgataa
    13261 gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt cacattatct
    13321 ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac accgtataga
    13381 acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg tacatagtct
    13441 ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg tcctcttgca
    13501 tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt ggtcatcgtt
    13561 tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt cgtttcagca
    13621 ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca atcgcttcgg
    13681 ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg tgttgtacca
    13741 ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt ataaaaataa
    13801 ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag gattttcatc
    13861 caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg aattagtata
    13921 atcaaagaat ctctactatg caatgttcat ttttataac
//
LOCUS       DACCYH010000075        13893 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.078, whole genome shotgun sequence.
ACCESSION   DACCYH010000075 DACCYH010000000
VERSION     DACCYH010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.078"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IVY85_002144"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IVY85_002144"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP8045150.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IVY85_002145"
     CDS             complement(1172..1867)
                     /locus_tag="IVY85_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP8045151.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IVY85_002146"
     CDS             complement(1880..2596)
                     /locus_tag="IVY85_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP8045152.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="IVY85_002147"
     CDS             2926..3534
                     /locus_tag="IVY85_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP8045153.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IVY85_002148"
     CDS             3859..4182
                     /locus_tag="IVY85_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP8045154.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IVY85_002149"
     CDS             4364..5734
                     /locus_tag="IVY85_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP8045155.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="IVY85_002150"
     CDS             complement(5864..7165)
                     /locus_tag="IVY85_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8045156.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="IVY85_002151"
     CDS             complement(7519..9510)
                     /locus_tag="IVY85_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP8045157.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="IVY85_002152"
     CDS             complement(9654..9920)
                     /locus_tag="IVY85_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8045158.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IVY85_002153"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IVY85_002153"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP8045159.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IVY85_002154"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IVY85_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP8045160.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="IVY85_002155"
     CDS             13264..13827
                     /locus_tag="IVY85_002155"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8045161.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
BASE COUNT         3986 a         2901 c         2564 g         4442 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagccattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaagtaaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaa
//
LOCUS       DACCYH010000076        13780 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.079, whole genome shotgun sequence.
ACCESSION   DACCYH010000076 DACCYH010000000
VERSION     DACCYH010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.079"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            112..306
                     /locus_tag="IVY85_002156"
     CDS             112..306
                     /locus_tag="IVY85_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045162.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            575..874
                     /locus_tag="IVY85_002157"
     CDS             575..874
                     /locus_tag="IVY85_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045163.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(971..1405)
                     /locus_tag="IVY85_002158"
     CDS             complement(971..1405)
                     /locus_tag="IVY85_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8045164.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1637..2347)
                     /locus_tag="IVY85_002159"
     CDS             complement(1637..2347)
                     /locus_tag="IVY85_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8045165.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2337..3197)
                     /locus_tag="IVY85_002160"
     CDS             complement(2337..3197)
                     /locus_tag="IVY85_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP8045166.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3202..3840)
                     /locus_tag="IVY85_002161"
     CDS             complement(3202..3840)
                     /locus_tag="IVY85_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP8045167.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3855..4154)
                     /locus_tag="IVY85_002162"
     CDS             complement(3855..4154)
                     /locus_tag="IVY85_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8045168.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4197..5681)
                     /locus_tag="IVY85_002163"
     CDS             complement(4197..5681)
                     /locus_tag="IVY85_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8045169.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5701..6162)
                     /locus_tag="IVY85_002164"
     CDS             complement(5701..6162)
                     /locus_tag="IVY85_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8045170.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6180..7247)
                     /gene="ulaG"
                     /locus_tag="IVY85_002165"
     CDS             complement(6180..7247)
                     /gene="ulaG"
                     /locus_tag="IVY85_002165"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP8045171.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7465..8235
                     /locus_tag="IVY85_002166"
     CDS             7465..8235
                     /locus_tag="IVY85_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8045172.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8508..9335)
                     /locus_tag="IVY85_002167"
     CDS             complement(8508..9335)
                     /locus_tag="IVY85_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8045173.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9903..11138
                     /locus_tag="IVY85_002168"
     CDS             9903..11138
                     /locus_tag="IVY85_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP8045174.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12137..12397)
                     /locus_tag="IVY85_002169"
     CDS             complement(12137..12397)
                     /locus_tag="IVY85_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8045175.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12424..13725)
                     /locus_tag="IVY85_002170"
     CDS             complement(12424..13725)
                     /locus_tag="IVY85_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8045176.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4429 a         2826 c         2172 g         4353 t
ORIGIN      
        1 aaaactttca aattgattct ttttatttat cataaagata tagatacata atcattttca
       61 cttacatata tttgaatatt aactaaaaac agcaagaaag aagaggatca aatgtattat
      121 gtagaagtaa aaacaaaagg cgtcaaaaac aaacaatatg tgaaaggtat cagtaacgaa
      181 tatcctttac ttggttcatg gaaagaagct gctccttttt ccaaaccatg tgccataaaa
      241 ataaagaacg agctagaaaa agaactgact tgtggtaaag ctgttgtaga tatcatcgaa
      301 aagtaaatcc aatgtataga aaggaagatc acacatgaaa agcgacgtac aaattgctaa
      361 gattatagaa ttagaagaag aagacgcagt gttgacaatt gaaaaaaaat aaatgtttgt
      421 ataccatatg attatttcaa tatgtatcct ggaattggag atctcgtaga gctatatcaa
      481 gatgaatcaa atatacttgt cattcatcat gaaatataga gacttataaa attgtttatt
      541 tttataatgt atcagtatag aaaggaaata aaacatggat tatactgttg aatttgacag
      601 tgaactactt aagcaatttc cacaagaaga acatttcatg tataaagaag caatttccca
      661 caaagtgaca gagtatttgg agcataattt ttttcgtata aagccagtgc gtactcgttc
      721 ggcaaaagga atatacgaga tgaaagtcaa aattaataaa aaaaattatc gcatagcatt
      781 tgctttaaaa aaaaatcatg tgttcatttt ttatattagt tcgaatttgc aaaaaattgt
      841 atttgataaa gaggtgagta aaaaaataaa atagctgagc attggaggat agattgagtg
      901 atactaagtc tatcctcttg tttttctaaa gaactaagct agaatttttt cttttgtacg
      961 ttattatatg ttatttgtca ttaccaaaaa gcaccttttt tcttctagta tctatgacat
     1021 aagaaatacc acccatttcc tttacttgaa caaatcctac tgattcataa aatttttttg
     1081 ttttctcagt atcatctgtc aataattcga tttggactgg tccataacta gtgatcttct
     1141 ccaaagcttc attcaaaagc actttgccta ttccttggcg ctgatacctc ggatgaatga
     1201 ggatatcttg aataaatgcg atcgtcgcaa aatcagtgat ccaacgaacg attccgataa
     1261 tcttcccttc cccattacgc tttatcagag attcagattg ttcaaatgct ctagctaacc
     1321 ttgcagtgtc ttttgtgtaa ttcgtccaac caacgctctt atataattca gaaatctcat
     1381 cgatcataag ggaatcaata ttcatttttt tcttcctttc ttatacaatt ctccctctaa
     1441 taatacagtt ctttgactat ttaatctatc acatataact ctcctgtaaa gattctctcg
     1501 attccttaaa attcctcttt ccttttagaa tgcatacttt ttgataaata aaaaggttgt
     1561 gataaatttt cttcacaacc tctgtttctc tctatcaagt gttatctttt aattttctac
     1621 ccttacccta gcatctctat tgcccgtagt atgcatactt tccatgtttg cgaaaataat
     1681 gtttatctag taaatattgc gggatttcac atatattggg gttgatcagc tcgttttctt
     1741 tggccatcag acaaatttca tctaagatca aactgttttc tactgctttc ataggtgaat
     1801 ccccccatgt gaacggtcca tgtccataga caagtactcc agggacttcg tttggatcta
     1861 attttctttc cttaaacgtc tcgacaatca cattccctgt atgaacttcg taagcttctc
     1921 ttacttcttc tttcgttaat tggcgtgtac aaggaacctt accataaaaa gcatcagcat
     1981 gagtcgtgcc ataagctggc aaatcgcgtc ctgcttgcgc ccacatgacg gagtgcgtgg
     2041 aatgagtatg agtaatagca gttatatctt caaaagtttg atacaaaaca acatgtgtgg
     2101 ggagatctga agatggtttt aagctatctt cttctattat atttcccgac aaatctgtta
     2161 ccaccatttg atctgcttgc atgcattcat atctgacacc gcttggctta atgacgatga
     2221 ctcctagctc acggtccact tcactcacat tgccccaagt caatttgact aatccgtatc
     2281 gcggcaatgc cagatttgct tcaaggacac gttgcttcat tgctgcaatt ttttcttcac
     2341 aagacatcat agccagcctc cttcaattta gaacttaagt agtcttttgc ctgctggatt
     2401 tctcttcgaa agtcttggct tttttcactc cacatttcaa tcagaaatgt tccatcataa
     2461 tctagacgtt tcaaatgtct taataatcca agaaaatcaa cacaaccgct accaaaagga
     2521 acatcacgaa actggcctga atattctttt gttacagggt aagtatcttt taagtggata
     2581 gcagtgatat aatcgatccc tttctcaagt tctcgagccg gattattttc cggccatgcg
     2641 gacaaattgc ctaggtctgg atatacttgt agaaaaggtg aatgaatctg ttctttgatt
     2701 tctaaaaatt ttgaaatgga gttcataaat ggatcatcca tgatttccac ggaaagaatg
     2761 atgccatatt tagaagcttc ctttacgctt tcttttattc cttcgataaa gtcttctctg
     2821 gaattcattg atttctcttc ataataaaca tcgtatccag ctatttggat tactttgacg
     2881 cttagattat gcgccaaatg gattgctttt ttcattagtt gtttcgctgc ttttttcttt
     2941 tctggatcag cagaaccaaa cggaaatcgc cgatgcgcac ttaaacaaat cgaatggata
     3001 cgtacaccgg agctgaacat ctcttctctc agctcagcta tcttttcttc agaccaatct
     3061 aatcgtgcta accgctcatc cgtctcatca atcgacatct cgataaaatc gaagtccatt
     3121 tcttttacaa gggaaaaacg ttctttccat gaaatatctt tgggtaacgc tttttcataa
     3181 atcccaattt gagccatttc cttaccccca gatacgaatg atctcatctt tgaagttttg
     3241 tgcggccttc atcggatcat ttgctgctgt aataccacga ccagtgataa aggtatacac
     3301 atcgatccct tcaaataagt gtaatgtgtg tggatttaat ccaccagtta cagaaacatt
     3361 aaagcctagt tcaatcagtt ttttgatctt cgataaatct ttttcacccc aagtttcacc
     3421 agataacagc gcatcacggc tttgatggta gattacctgc cggataccga tgttatgcca
     3481 atccatggct tgttcaaaag tccaattgcc gtagagttct acttgcaatt cacctatttc
     3541 tttttgtgct gcctccatag ttggaatcgt tgcacaacaa atgactgtca tccagtctgc
     3601 tcctgctttt gcaacattac tcgccactgt tcctccagcg tctgcacatt tcgtatcagc
     3661 gactagtttt ttatctggga acatactgcg aatacagcga atcgcttttt gtccttcctg
     3721 taagcaaaga attgtgccca cttcaataat atctactaca tcaccaactt tcataacatc
     3781 tcctaatgca tgttctaagt cattgtgatc taacgcaatt tgcaaattcg gcctgctcat
     3841 cttagttcct tcctttactt gtttttttgg taccactttg tttcagcaaa tttttcactg
     3901 atttctttga ctgacatcac atttttgata ccgataactg taactccttt tttctctgcc
     3961 tgagtaaaca tattcaaaaa attgactggc gtaaaaacta catcgtattg gccagctgaa
     4021 ctttttccct ctgaaatggc acaatgatgg attttggtaa ttggtacgtt tagttctttc
     4081 attgcttttt ccacacttct catcatcatc aagcttgttc ctgatccatt tgcacacgat
     4141 actaaaattc tcatcttaat ccctccatta tgagtgttgt ctaatcagat tttcttttat
     4201 actctctgct tttctgcata tgtgtcatag tcttctacaa tcaaaaaata accatcagga
     4261 tttttccgat attgtaactg cggaatagcc aataaaccaa tgacaactac ggcaattcca
     4321 gcataaccta ggactttcat gattactgta aacaatggcc aagctgttgc ccagtcgaac
     4381 atcccgatat aaccaccgta tgttgacatc ccaatccatg tagcgaataa tgctgaaccg
     4441 cctacttgta tgattcctga aagaaacggg aacaggcaag cggctttgaa tccacctcgg
     4501 ttattggcat aaactgctat aacggcatta tcaaagaaca acggaatgaa accggctatg
     4561 acgatcgttg gtgatttcaa taaaatcagt aaaccaatca tcaaaaactg tcccaaagca
     4621 ccaaacaaga acccaatcgt taccgcattt ggcgaaccaa agccaaaagt agcagctacg
     4681 tcaattcctg gaacagctcc tggtaaaatc gtgtttgaga ttccttggaa agattgagtt
     4741 agttcatcga cgaatgtccg aacacctaat tgcaagatcg ccagatatac cgcaaaattt
     4801 aaagaagtcg tcataatgta gaataagaaa ctctgccctt cttgcatgaa ttgcgcttga
     4861 atcaaataat ctttgcctag tacaatcaga atgatcccaa agaaaaatag catgagtaac
     4921 gaagtagcaa ccatattctc attgaaaatc gacaagaatc ctggtaattt gatatcttca
     4981 atttttttgt cttgcttgat tgatctattt ttcttttcat cccgcttttt catccactca
     5041 gctaattttg caaatataaa gattccgaac atttgctgat gtgccactgc aaatccggca
     5101 ccttctgtca attcctgcgt gattcctact gttaagttag aagctactgt ccaataacaa
     5161 cctaaaatca gtcccataaa aatcaatgct tgtatcctac ctagatcagg aaagcaaaac
     5221 agtaaaatcc aaaaagcggt agctgcttgt tgaacctgaa cgtttccggt agtgaaaacg
     5281 gaacgcagtt tcgtatattt ctgaaaacgg actaataaga tatttaaaac aaatgcaatc
     5341 agtaataaaa tcatcacatc tccaaaggta cggccaaatg tctccattaa tccagcatcc
     5401 acagcatttt gtccaaaata aggatctgta accatggcat ccaaattgaa ccgttccttt
     5461 agtccaacca agattggccg aaaattattg accaaaccac cagagccaac agtcaaaata
     5521 aagtaaccga ccgttgcctt taagaatcca gcgatgcatt catgaagcgg gcgtttcagc
     5581 aaaatatagc ctaataacac gataaaacca atcaggtatg ctggctgcgt caagatattt
     5641 gttgcaaaat acgtccaaat gcttaacaaa aattccccca tcttttttct cctctatcaa
     5701 ttaattatat tctgccatca caacttggta atcatcgatg gagttcgtag agatgagacg
     5761 ctcaaccaat ccatctgtca ttaataattc ggataatttt tggatatttt ctagatgttc
     5821 ttctggatct tttgctgcga gtgtaaaaaa caaactagcc gttttctcct cattctcccc
     5881 ttcactttca aaacggattg gctgtttcaa cttggtaaat gaaatagctg ttccaaaaac
     5941 accatcactt ttttctgaag aatgcggcat tgctacttga ggtgcgatca caatataagg
     6001 accgtatttt tctacacaat ggatgatctc ctccacatac gtttcatcaa taatttcctt
     6061 atcgatcaat acaagacagc tttcggcgat agcttcacgc caatccgtga tttctttatc
     6121 tgggtaatga atcaattgat ggtcaaaaaa gtatttcagc aagaaactcc ccttctttct
     6181 tataaaaacg acggaaacgg tgtatcattt tcaatcgaga agacatcttc aaatcctcga
     6241 taaaaattaa actcaagatc atctttgttt gtcggataga tgaatttccc gcctacttgc
     6301 cagatataag gtttaaatcc atacttcagg cgatcctttt tgaatttcca aatttcagtg
     6361 atctcttttg gatcagccat aaaattagac caaatatcgt agtggacagg aatcactact
     6421 tttgcattca atgactccgc catgcgcagc atatcaacag aagtaacttt gtctgttatc
     6481 cctcgtgggt tttcaccata ggcccctaaa cagacatcga ttttgtgttc attaccatgt
     6541 ttggcaaata aattggagta atgtgaatct cctgcatgat aaagattccc tcctgatgtt
     6601 tcgaataaat agtttactgc aatttgatcc atctcctgag gcatttttcc ttttaatacc
     6661 tcttctggat cttcacaagt gaccaacgct gtccgatcaa acgcttctaa agctactaca
     6721 ttgatgtctt tgattttgac tgcatcacct ggctttactg taatcgtttt ttcttcagga
     6781 attccccatt tttgccacgt gcgaaccact tcttttggcc caatgaatgt tgcttcagga
     6841 caattttgat gaactgctgc ggctgtatgg atatctagat gatcggaatg gatatgtgta
     6901 acgattaaag cgtctacatt ttttatcgca aaaggatcga ttacgaaagg ctgagtacgt
     6961 aaattaggct gcatattttg aacgccactc atacgcatca tctgatggcc ttttttcatc
     7021 atgccattcc catgactacg ttttcccgtc ccacaccata gatcacataa aatattcgtt
     7081 ccttcatgac ttttcagcca aattcccgta cagcccagcc accacatagc aaaagttcca
     7141 ggatccacta cttctgtttc gatttcttca ttcaagtatg tgccccactc aggaaatgtt
     7201 tctactaacc acttttcttt tgttacttca ttgacagttt tactcatttg cttgctcccc
     7261 ccatatcgtt gttccaactt acaagtaaat attaatgtgt atcagcatcc atgaaaaccc
     7321 ttttatatga ccgtttgttt tttatatgat tttctttcag taatcaatga ttgtttcagt
     7381 ataataaaga cagatatgaa tgtttaaatg aacaactagt caaataaatc catggatcaa
     7441 gatgtccctc aggaggcaaa agtcatgaaa aactcacttg tcaatattga aaaacgtcaa
     7501 aaagaaattt tggaactgct tcagcagact gaacaactat caactttcga attagctgaa
     7561 tcccttgatg tatctattag tacgatacga agagatttaa atttattaga agaaaaaaac
     7621 gagatcatac gcaattacgg ttattgttct tataattaca aaaaccagac tgattttgat
     7681 caatctggtc ctgaaaggat taaacaagcg attgccagag aagctagtaa atacattaac
     7741 gattatgata cgctgtttat caattctagc tcgactgcgt tgaaaacact ggattttctt
     7801 caatctaacc atttgacagt cattacaaac aacgtgaaaa tcaattactc tccgcacaaa
     7861 gataattgta gttatatttt aactggtgga gagatgcgct ttccaaaaga agcactagtg
     7921 ggcgacatcg cattaaatac gatttcttca gtcaatgcag acatttgtat cattggctgt
     7981 agcggagtcg atgtagtaca tggggtcaca acaaagatat taaatgaatc aacgatcaac
     8041 gaaatgatgg tcaacaagac aatcaaatgt aagattttag tcgctgatca tcgaaaaatc
     8101 ggactgacat caaagttcaa gattgctgat atttcagtct ttgattattt gatcactgat
     8161 aaattttgtt ctgaaaaagt attagaggag atcagaaata cggggataaa aaccattcaa
     8221 gtagattcat tttaagaacc aggacgtaaa tatgtaaata ggattatcaa aaaaatgaat
     8281 aacaaattta tcgataaatg aaaaacacgc tacgctcaaa ttgataaagg aatcccaaga
     8341 tgtatccatc caatcgatac catcataatc tattgcttct tttataaccg ttatatgttg
     8401 cctaaatagg aatcaaacaa tatcgaaaaa aggaggtatg acaaaagtca cacctccttt
     8461 tttcgaataa acggtattca aatcagaaca ctgatttcac aaccacttta tttcaggtcg
     8521 ttttcaatca cctttaccgc gtaatcattt ccgttgaaac gcgctatttt ttttgcgtcc
     8581 atatatcttt ctaaataatc atcttctcct aattgcttat ggcaagaagc aatttcataa
     8641 agcaagtatt ccaatgcata agtcgattta tgtttcttat tatactcaat acccttctca
     8701 cataattgga ttgctttatc ataatcctcg attttacggt aataggtggc gatatcataa
     8761 tatacaatgc agaccatcag attatcaaaa tcagtctcac tcaatatttt aagtgtacgc
     8821 tccacataaa ctttcgcaaa atctaattct ccttttgaca agtagatcat tgccacgttt
     8881 gcagctgctt gtaagtgatg gatatctgtt tcggtcgttt gttccaacac ttgattaaaa
     8941 taaaataatg ctgcatctgg ttttttcgca taaagatgat caagcagtcc ttgataaaat
     9001 aaaatttgat ttaaaagatc agaattactt actttttcct gatcgatcgt ttgtaaaatt
     9061 ttataagatt cttggtattt tgatacagaa ttcaatgatt gaatctcatt tatttttttc
     9121 ctgattttgt cttcttctgt ttctactaat atatcggaga tctcgacatt cagctttttg
     9181 caaagcgcac ttaagatatt gatgcctggc gacatattct tgttttcgat attgctgaca
     9241 gttgcttgtt cacaaatgcc ttctgctaat tgtacttgtg ttaacccctt tttctttcgg
     9301 tattctttta gtaatgctcc atctacttta atcaaaacaa tttccttccc tttaaaaaat
     9361 tatttctcct ttatatcata cacaaaaaaa agttctaaac aaccaaaaat gtttagaaca
     9421 gaataaaaat tttaatttta aaataatatt caaattaaac aactggcgta tctgctcctt
     9481 gatttctttt tgtgttatct gaataattat cgtttacagt tataacacat agtgaaaatt
     9541 gtcatggcga gaaagtctaa gaatattttt aaacataact atttctttct tacgtgtgta
     9601 taatgacgac agcacaccta tcaattaata taattatttt atttttttat gttctcatgt
     9661 ttatttttaa tgacacaact tctcgtattt atctggcaca acatataaat cgatcaaaaa
     9721 attgttgcgt gtttttttga aaattggagt ttcaacttga ttacaaaatt taaatttaga
     9781 agttgtgaaa acagcgagag acagtttaat attcaaaata tacataggct actcgaacgc
     9841 tgtgtttttt agtatactag taagaaacga agggatagac tcattccaat aaggaggatt
     9901 tcatgaagac aattcttttt gctcctgcag tattcaattt agctgagaca acacgaatga
     9961 ttgaggttgc caaagctatg aaagaccgtt attactgtgt attctttggc ttttctaaaa
    10021 cttttgctcc tttgatcaca gaaagtggct ttgagtatca tttactagcg ccgatcctta
    10081 cgaaaaaaca agaagaacaa atcataaaat ttgaccagtt gcgtacactt aaaaaccctt
    10141 ttaccaaaga aatggttgcc tctcgtatca ctagtgaaca gagtctgatt gctaagtttg
    10201 agcctcgact gattgtgaca ggttcgaatg tgacaatttt tttatcggcc cgcaataaaa
    10261 agattccttt agtatatgtt aaaccctatg ctttgagtga accttttttt caatcagagc
    10321 atgcgctgat gccccacgag ttacgtttct tcggcccttt aagcaatccc ctttggcact
    10381 ggacaaaaaa gcgtatagta aatacccgat ggatccccaa tgccttcaaa cacgctgcac
    10441 aaaaagaaat gcttccttta ccaaaaacaa gtttacaaat gatggatgct gatctaaatc
    10501 tcatcacaac gcctaaacta tttttggaag aagaacagct gccggaaaat tatcgaatcg
    10561 ttggtccaat ttttgctaag ctcagtactt ccttgcctga agaagtgcta acttttatac
    10621 agcgaaagca acaagaaaga aaacgtatta tctatttcgc aatgggtagt tcaggcaatc
    10681 cgcgtatgtt acgcaaaatt ttgttcttct tacaaaaaca accggatcta gcggttattg
    10741 cacctattga ctatctgctt gatgacattc aatctgactc agaagatctt ttaataacag
    10801 aatatctgtc tagtcatctt ttgcatgaat tcattgattt ttcttttatc cacggcgggg
    10861 aagggaccgt ccaaactgcc tgtgaatccg gcaaaccgtt tatcggtgtg gggatgcaat
    10921 ttgagcaaag ctgtaatatt gactattgtg taaaatacgg aaacgcaatc gccctcacaa
    10981 aaccagtgac tgaaagaaaa ctgcagattg ctctgcgcga agtttcttca cccaagattc
    11041 gtcagcaggc acgcacgctg caagccgttt ttccaataaa tggtccacaa caagcaattc
    11101 aagaaatcga aaccttcctg agcaaccacc caatatagtt tcaagtagaa aaagcttttt
    11161 taatagagac tcatcagtaa tttcgctatt cgagacccag acagtgaaaa tacaaaaaaa
    11221 gtaaatagtg gattgaaaag ttagagttac ttaaattacg gtgtctaaaa aacacaaaat
    11281 catccttaaa ataaaagatc tgacgctatc caaatgagca taggtagcgt ctgccattta
    11341 ttcttagtca aattctttga cacctttctc tggttggatc atctcaacgt ttcttcaaaa
    11401 gaaagaccat aataattagg caaatacatt ttcttaaaat gtgtcatata gatctagaag
    11461 catcgctttg tagagatttg tgccgctagt cccctcaaca attactaaat caaatgtagg
    11521 atttaccact ttatctttga taatcaacct ccctcttctg ttgagacaat gatctcgcaa
    11581 tccgatgcat actcctatat aaactgccag caatccccac atattgagta gatcatgtca
    11641 gaatgttcta tgtcgtcagc aacgatacac taaaagtcac gttcaccatg actgattgct
    11701 attcccagca caattatttc tatacacgtt tcatgatttg aataaacact cacatcatac
    11761 atattaccat tcatacacca tactgcagcc cctactatat ggttgtaagc tcccaaatct
    11821 atatttgact tgatcacttt ggtagtagat ctactaattc taaatccatt gctgataaaa
    11881 tttccatttt ttcctccttc tttctgccaa aacagttaaa acaataataa tctttatgta
    11941 aaaaagggct gtgacaaaag ccttgtcaca gtcccatatt ccgaataaac ggtggtcaaa
    12001 agcttgcatc tgcggtaata agttcaacca aacgaaaaat aagccaacat ttcacaaaaa
    12061 tttaagaagc aattttcgta aatttcttct tatttcttga agctaaccac ttctgttaca
    12121 accactttta gaacatttaa agcttcgatg tgacaaacca tctatgctgc tcgatcataa
    12181 aattgatagc aaaaattcca caaagtacat atgtccccca tgaaaacaaa acattcatat
    12241 gcggtacaat gatctttgga agaatcacga tcaaaaatga tgccagtgcc caaatccatc
    12301 gattgatttg aatatgatat gtagctagca tattcaatat aaggtgacta gccgttaata
    12361 aaaagataaa aacaaaaaat aaaatcagcc aatacattgt ttcccacctt ctaaaaacca
    12421 aacttagaca gtctggactt cgttttctcc acgactttgt tcatctgaca gcatcttttt
    12481 atcataagct tttacaaatg gataatagat aataaatgct atcaatgtgt tgatcatgat
    12541 caagataaca acacgccagt catttcctgc tgaaagataa ccgccgattg gagctggtaa
    12601 tgtccatgtt ggcaaaacag taaatctatt gaccatttct aatttgactg caaaataact
    12661 gataatagtc aaaactaaag gaactaagtt gaacgggatg aacatgtaag gattcaacat
    12721 cataggagct ccaaaaatta tgggctcatt gatattgaag ataccaggaa taatagaaaa
    12781 acgaccaacc tctttcaaga attttgattt acagaagaat ataaacaaaa cagataccac
    12841 gatcgttgct cctgcaccac caattgttac cgtccattga taaaactgtt caggagcgat
    12901 atagggtaat tgggtggcag ctgtaccatc tgccaatgcc tttgcattct catccaacat
    12961 gatcagccac attggacgaa caacagcccc tacaatactc atgccatgga taccaaatga
    13021 ccacaacaaa tggatcaaaa acattggtag taaaacacca agcaaattat tccctaaaat
    13081 attggttaaa ggctcgaata acgataataa ggcagcgttt acatcaaagc ccgcgacgct
    13141 tcgaatcagc caagtaattg ttaaaatgac tgcacctgga atcaatgcct caaaactacg
    13201 ggcaacagca ggtggcactt gttctggcat tttgatagta atatttcgat ttttaaagaa
    13261 acgatatact tccactgcaa aaatcataga taagatcccc gcaaacatcc cagatgctcc
    13321 caaagaactc ataggtaaga cccagccaag aggtgcctct tcaggagtta aaacgtttac
    13381 aggaacttgt aacataaaga acgttcctaa agataatata ccacctgtca cactatctaa
    13441 tccatatgat ttagctagat ccgaacccat aacaaaagtt gcataaatag ccatcaatcc
    13501 catcgtcatc ctaaagggta aaacaatcgt tgccgaataa ggggcaataa attcattcca
    13561 tccaggaaac ggtaaattga taaagaccat aaaaaatgtc ccaatcaaaa tcaatgacaa
    13621 tgttgaaaca accccattac gaattgctaa taaatgacgc tgtgtaccaa tttttgccat
    13681 tggtgggaca agtttctctt cgacccattt catcagcttt tccactaaaa ccactccttt
    13741 tcaataaaat atgaaccatt cattgcatga caacgcaaag
//
LOCUS       DACCYH010000077        13615 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.080, whole genome shotgun sequence.
ACCESSION   DACCYH010000077 DACCYH010000000
VERSION     DACCYH010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.080"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(232..924)
                     /locus_tag="IVY85_002171"
     CDS             complement(232..924)
                     /locus_tag="IVY85_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8045177.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            1009..1185
                     /locus_tag="IVY85_002172"
                     /pseudo
     CDS             1009..1185
                     /locus_tag="IVY85_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1361..1930)
                     /locus_tag="IVY85_002173"
     CDS             complement(1361..1930)
                     /locus_tag="IVY85_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8045178.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(2004..2327)
                     /locus_tag="IVY85_002174"
     CDS             complement(2004..2327)
                     /locus_tag="IVY85_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045179.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2481..3293)
                     /locus_tag="IVY85_002175"
     CDS             complement(2481..3293)
                     /locus_tag="IVY85_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045180.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3647..4495
                     /locus_tag="IVY85_002176"
     CDS             3647..4495
                     /locus_tag="IVY85_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP8045181.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4640..5581
                     /locus_tag="IVY85_002177"
     CDS             4640..5581
                     /locus_tag="IVY85_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP8045182.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5571..7742
                     /locus_tag="IVY85_002178"
                     /pseudo
     CDS             5571..7742
                     /locus_tag="IVY85_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7854..10025)
                     /gene="copB"
                     /locus_tag="IVY85_002179"
     CDS             complement(7854..10025)
                     /gene="copB"
                     /locus_tag="IVY85_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP8045183.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(10045..12231)
                     /locus_tag="IVY85_002180"
     CDS             complement(10045..12231)
                     /locus_tag="IVY85_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP8045184.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12231..12440)
                     /gene="copZ"
                     /locus_tag="IVY85_002181"
     CDS             complement(12231..12440)
                     /gene="copZ"
                     /locus_tag="IVY85_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP8045185.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12453..12893)
                     /locus_tag="IVY85_002182"
     CDS             complement(12453..12893)
                     /locus_tag="IVY85_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP8045186.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12967..13506)
                     /locus_tag="IVY85_002183"
     CDS             complement(12967..13506)
                     /locus_tag="IVY85_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8045187.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3833 a         2843 c         2416 g         4523 t
ORIGIN      
        1 gtacatccta aaatataagc tatttgttta cttctgtaga atatatactt tgttgttaga
       61 taagttgcac atgtttatag aatctaaata ataaattttt atgtaccgcc cctcaaagtg
      121 aactgaaccc caaaagttga actatttaat ggactgtttc cgatattcta ctggagatag
      181 tccattaaat agttcaactt ttggggttca gttcaaagag ggggggattt tttatcgttg
      241 tggaatattt aaataccaac gcttgtcatg aaaatcttgt gctccaccct tagtatttcc
      301 ttctggatcg tttgtcgcac gcatcatgac gtatactttc ttattaggga aatcacgcat
      361 attgaaagat acatgatagc caacatttcc taaagtatta taagcttgat ttacgtctgg
      421 tctataaatc ccatcagctc ttactctagc tagttctttt ccagtattgt agtccataat
      481 gaaaatatac tcgtatttat aattagcaat gtgccatcca gctacatgca agtttgcgtt
      541 ttcgatttct ccgaactgat caatgtgggc gtaatttgtt ccatctgtca gcgtagaatt
      601 tgcagcacct gctcgagttg gatcaattac tggtttatcc tccgaagtag ttggattatc
      661 atccgtaaaa ccatgagcca aatcataggc tagtttttct ttacttacac ccatttcaga
      721 aagataaccg taaggatctg tatgatcgcc ccaaatattt tgtgttaccc ataaatgcga
      781 tttgattcct ggttggttat aaggagtgtc taatgttaat ggaataccat atttcattgc
      841 tgaatctctt gccaattcaa cgtatacttt atagttcttt tcaaaagttt ctttatcatg
      901 tgtatgttgt aactcgattt gcacaggact tttggctcac gaggttgtcc tactctacat
      961 cttgctgata gatatgctga aaggaaaaca ccaacattat gttttgcttt ttcagctaga
     1021 acgagacaag caggaatacc tttagaagat atcaaaggtt ttttaggtca taaagatgtt
     1081 tccactacac aagtctatgc ccatatttct cccgaagtga aaaaaagatc aatgaatcaa
     1141 ctagaaaact atatagaaga gcaaataaaa aagcactcaa attgagtgct tttttaagct
     1201 cactatcact aaaactatca ctaattttat ttataagcga acatgtttct atcactgatt
     1261 ttttaaatct agcgaaaaac ccttatctta aaggcgttcg ttcaattctt tggcaaggtc
     1321 ttcaaagcct ggcttgccta gaagtgcgaa catggttttc ttatagctca tgtagaaaaa
     1381 ttttcttgat gatgatttta tccatcaatt gagattgact gttttgctct cctatgattt
     1441 cagctatgat tgtccgattt tgcggttttc ttcttaactg gtagttgatt ttcccagttt
     1501 ccatcaactc gctaagcata tccacgactc cgccaaatcc ggtctttttc gtaataaaac
     1561 ctagtgctac cattcgttca tctagatttt ttgctgtaat cgtcacacca gtaatgagcg
     1621 agtgctgtaa tgtttgggcc agctcttcca tcccttcagc attggatacg acggaagctg
     1681 ttttgttaat aggttcttcg tttatttctt ttggttcttt cctctccact ttcactgttt
     1741 gacgaaggac atctagttct tgcaagagcg tttcgtattg ttcttgggtc agcatcacgc
     1801 cagctacttt ttcacgatta aatatatata cgccagttcc cgcgtctcgc gcttgagcaa
     1861 atacatccat cggcgatcgt ttcacttctg tgatcgaaga tgtgggaact tctaatttct
     1921 ttaattccaa tggatttcct cctttactaa tcgcttttta ttatagcaca gactgacttg
     1981 ttttgatctt ttttccaaag gttttatcgg tttttgccta accagtaatt gatatcggta
     2041 agaagtcttt cgataccaaa tgaaggatgg tacagatacg ttttttgatc ttcgttatgg
     2101 tctagtggaa aaaaatgatc gatgatcact agatctgcat accgaggcgg cttatttcga
     2161 gtacatacat gtatagagat ccgttttcga tgtcttagcg ttttgttcag taccccagca
     2221 agtcttcttg cttcttggtg ttcatagcga aaaaaaaaga cgattttcac ttcaggaggg
     2281 tggacatcta aataccctcg ttcctggagt gttttcagta aaagcatccc ccactctact
     2341 gcggggtgtg caagtgcttg ttggatcgct agccgaaatt gatatagttt atctttggaa
     2401 ttgggaaatt tttctaatgt ttcctgataa taacgaactt cttctatcag ccggtcatgg
     2461 ataccaatta cgtaacgaag tcattcttgg taaagtactt ttgtcagctt tgatttgctt
     2521 tcgctctcaa attcgctatt cagccaaaac agcgaatcaa gcttttcagt cacaatcgtc
     2581 aatttccgat agtcttgtgt ctcttctatc cgtttccacc gcttttcagt aatctggtta
     2641 cagctcaagc gcaaaattgc tagataattt ttttctttta ttggcaaaat ctgctcctct
     2701 ctaaatagca attcaggaaa caattcaaag aatagtgttc gactgtctcc taaaatctcg
     2761 atttctgccg gatcctttct ttgcttcaat aattggatga acatctcaga aaaggactgc
     2821 tctccatcat ctgcaaaaaa atgcggaaaa tctttcaaaa agcaaaacag cagatatctt
     2881 acttttcttt ctgaaccaaa gatctgtgct tttttataag aaaaagaaag gtggtactcg
     2941 ccaaaccact ttctcaatcg ctcttgcgta gaacgatcgt acttctgctc gtaaggttca
     3001 ttcaatagca gatttttcag taaacaaaac gggggtgatt ggatcagaaa aacaaacaag
     3061 agataaggtt cgctatatcc ttgatcataa actaactgaa cggttttctt atgaagaatc
     3121 agctgaatgg gacagtcgct ttccgccaaa cgtttcagat gccgatccac tgttttaaca
     3181 tcgattttca gttcttcagc taaatcagct tttcgccaga tcaaatcttt cattagattg
     3241 attagtgaaa ataaattctg tgcatttttt gataatagtt tttgccatct catcgatcat
     3301 ttctcacctg atttctaaac atattgtttg atcaaaatgg gttcactcct aaaaagataa
     3361 aaaactccct ttctatttat ttaaatacgc aaatcttcct cattattttt tattttactc
     3421 aatttaatcg tattctcatt tcaaaacgaa tgtactttaa aaaaacataa taagatcact
     3481 ttgcttgtag gatgtattcc cttccattac aatgaaataa atagcgattt tatgagaagg
     3541 tgatgtgaaa atggaagaaa aaatagaaga atccaaacaa aaaatcgatc agttgatata
     3601 agaattagaa aaagcggtca tcggcaaacg ttcaactatc caattgatga tcgtggccct
     3661 attagcaggt ggtcatgtct tattcgaaga tattcccggt gtaggtaaaa cgttactggt
     3721 caaagcacta gcaaaagctg tacagggaac cttcagcaga gtccaatgta ctcctgatct
     3781 gctgcctagt gatattcttg gtttttccgt atacaattca caatcaaaag agtttgaatt
     3841 tcgtccggga ccgatcttta ccacgatctt gttagcagat gagatcaatc gtacgactcc
     3901 tcgtactcag tcagcacttt tagaagcaat ggccgagaat catgcaacga tcgacaacaa
     3961 tacataccct ctagacaatc atttttttgt cttagctacg caaaatccaa tcgagtacga
     4021 aggaacatat cctttgccgg aagcacagct cgatcgtttt ttattccgac tacaaatcgg
     4081 ttatccttct tttgatgacg aacttttgct acttttggat aaattcgaaa aagtgttgga
     4141 taacttaaat gaagtattga acagttatga aatagaagaa ataaaaacaa atgtggcgaa
     4201 tgtctttgtc agtcccgaag tggcatctta tgccttgcag ttagtgtcag caacaagaag
     4261 tcatgctgcc atccagcttg gcatcagtcc acgaggaagc ctttccttta ttcaggcagc
     4321 aaaagcttat gctttaatcc atgggagaaa ttacgtcacg cccaaagatt tgcaggattt
     4381 agttccttac gtgttttccc atcgcctgat tttttacgat cgttcattga aggaagacga
     4441 aaagaaacaa atcattcata ctcttgttgc acaaattcct attcctgtga ggtagattga
     4501 ggtagacaat ggaaaaaatc aaagattatt tcattaattt tgtgttattt ctggtatatt
     4561 tcatcattgt gctttttgct cttgtattca acaattcttt aggctggttc ttgttggcct
     4621 tttttactct ttatgtatca tggagcttga tttcattaag cagctttcta aagaaacttt
     4681 cggttacttg tacattgcag ccactcgttt atcgcaaaaa aagtatgcag gttatttttt
     4741 ccatagcaaa aaagagaact ttttctttac ctctccctcg tttacaaatc ggcttcccag
     4801 caatctttgc agaaaaaaac aagagatttt gattcttaca aataaaccgc aagaagtcca
     4861 attgctttgg cagccaaaag aaagaggatg ccatgaaaca ttaactgtca tttttacagc
     4921 ctatgatagt ctaggacttt tcagaaaaag tgcaaaaaga gagatctctt ttcctgtgac
     4981 tgttctgcct gctttttgta agattcaagc agaaaaatta cagagagttc tggaaaaaaa
     5041 acagcagctg aacgcgtacc aaagaggtct tgattttcgg gaacatcgcg cttatcgtcc
     5101 aggtgattct ttcaaccgaa tcgattggaa attgtctgct catactcaag agtggttata
     5161 ccgtgaatac gattatcaag aagaagaatg ttctcctctt ttttgctttt ggggttcgcc
     5221 cagtccaaaa tttgaacctc tgctagactt gtatttttct ttatggcatt tgagaaaaga
     5281 aaagcagccg gaacttctga tattaggcaa ccgcgccttt catggcaaag accccggtca
     5341 tacctatttt gcttctttgg aagctggcga tgagaaagct tttttcgcac acttgaaaaa
     5401 atatgcaaaa aaacaaatcg tcctctttgt tcctgagaat tctcctgaag tctcagaagc
     5461 tattgaaaca ttgtcaaaag atcgaacggt ttatcttgtt tatttcgtag aaaaacaact
     5521 catggtccaa gaaaaagaca agatatgttc attgccagga ggtgaatgga ttgacgatta
     5581 aacgggaaaa atggctgcgt ggcgtttact cctttgtgat actaagtgta ggtgctccga
     5641 tatttgtagt agctaatcaa ttgcaaaaca gctaccttct tatcctaact attggtgtcc
     5701 tatgccttat tgtctgctgt attcctaaaa aatgggtacg tttgctgctt tctttgccag
     5761 taatgatagg ttgtttgttt ttatattttc cttcgaagac attttccata ttatggatct
     5821 tacagttttt ttcgtcagtc acagaggaat tcttccatct atcccaaggt aaactttatt
     5881 atttgccaga aaagacagct ttttttctca taatgttgct gatctattta tttattacat
     5941 tgaccgtgga ttacgactat tggtacgcac cctttctttc tttgatggct tacttcatga
     6001 tgcttaccgt ttttcggaaa aaagatttac tgtacgaaat gattgcggta gtaactgttt
     6061 tgttcgtgta tctggcagca aggcaatttt tctccatccg gttgttgtcg ggaaacactc
     6121 gattccagtc attgactaca gttctgctta tttgtttctt aacttttagc gctatcttgc
     6181 ctttatatct tccgaaagta caccaatctc tcgaggaaac gagtgaaccg atccgagaat
     6241 atcttaatga tgaagggctt tatgacacac tccatgagta tcaattaacc ggaagtgcaa
     6301 gaacaggctt cagtgagaat gatgaacagc ttggtggacc tttatatgat aatggcgaag
     6361 ttgtttttac agcgaaccaa aaaggaaatg gctattggaa gatcgaaaat aaaaatcatt
     6421 acaccggttc tggatggata gaagatcagg acgcacccat ccaaccaatc caagaattcc
     6481 ctgttgacat cacgagctac caaaccgtta tagaagacaa agaaaacatt acattgaccc
     6541 taaaaaaaca agttttctta ccttatcctt atggaaatac taccttgact gatttttcat
     6601 tagctagtgg acaaacgctt tactatttgc ctgaaagcca gcgattcatt cctgtcccga
     6661 atgaatcgac acgttctgca actttacaaa cagaaaggtt agtttttacg agagaagaat
     6721 taaaaagcag tcagttaaaa gctgatgatt tagaaaatca gcagacaaat cttcaattgc
     6781 caaaaagttt gcctaaacgt attgaggact tggcggtcga attgaccaaa aatgaagcaa
     6841 ctttaattcg taaagtcgaa gctgtctaac agtatttaaa aacaagcgga aatttccgct
     6901 attccaaaac ggataccggg catactccaa aagaacacga ttatgtggat tatttcctct
     6961 ttgattcaaa agtcggctat tgtgacaact tttcaacttc tatggtcgtc cttcttcgaa
     7021 cacttggtat cccagctcgc tggacaaaag gttttacaaa tgggctcaga acaggcacag
     7081 ataatcaagg gaatgaggaa tacagcattc tcaatagtca tgctcattct tggccagagg
     7141 tatactttcc tggatttggc tggcttccat ttgaaccaac cccttcattt gccaatccaa
     7201 tgggttcaca accagttgct gaaacaaccg aaaccgtcca gcctgctatc gaacaaccaa
     7261 atgaaagcga aaatatggca gaaactattt ccagtgagag atctgaggtc attccatcag
     7321 aagaaacaac actcacacca gcttataaaa tcgtcttatt cagccttgga gtgtgtttat
     7381 ccgtcttgtt gttctttcta ttgttattca gaaaatctct gcgctggaca agttatctta
     7441 tctatcttca tttttttgct tctactaata cgattgcata tcaaaaactt cttaaccctt
     7501 tggaaacatt ggtaccgcgc aaacccgcag aatctttgca ggaatatagc ctgcgaatag
     7561 aacaagaatt caagctagat ctgcgtttca ttcgattaac tactcaatat gaagcagaaa
     7621 tatatggacg acaagcagat ccggtaaaat tcgataaaaa aacctattta gaaataacgc
     7681 atataataag aaaaatggta tacaaaaaaa ttctgacaaa agggagccgc ttcaaaaaat
     7741 aagataaaat ttctggagaa tctacttctg gaatgccgag aaccagctga ttgagtatcg
     7801 attactcgaa aaaaaggttg tgacacgttt tttgtcacaa ccttttttca ttattatctg
     7861 attttcaatg tcaaagcatt tagagctaca actaccgtac ttaacgacat caagacagca
     7921 ccaactgctg gacttaatat gataccaatt ggcgcaagaa ttccggctgc tagcggaatt
     7981 gcaagaatat tgtaacctgc cccccaccag aggttctgga tcattttctt tcgcgtttgc
     8041 ttggctagat caagaaaatg caaaatatct tttggatcgc tgtttgtcaa tacgacatcc
     8101 gcagaatcga ttgcgatatc tgtccctgca ccgattgcca tcccgattga agcacgggct
     8161 aaacttggcg catcattgat cccatcaccc accatgatta cttttttccc ttggtcaagg
     8221 tatttctgga caacagcttc tttatcatct ggtaataaac cgccataata ttcttcgatc
     8281 cctaaataat ctgctactgc ttgggcagcg tttttattgt caccagtaag catgacagga
     8341 gtgatttttc tttctttcaa tgttcggata aattcttttg cttctgtttt tactacgtca
     8401 cctaaagcaa cgattgcagc tagatgattt tctaaaatca ggaagctgat ggtatttcct
     8461 tgttcttggt aaggtttcaa taatgcttgt tctacgttta atccgagacg ctcaacttct
     8521 ttttcattaa ctattttcac ttgttgtttt ttgactgttg cctctagccc gactcctgaa
     8581 agatttttta gattttcagc ttgataaggc ttgatttcat gttctttcaa ataattcata
     8641 atgccaacag ccaatggatg attggcattc gcttccaagg ctcctagata ttgaaggatt
     8701 tcatttttat tgaacgcatc atcaagtact tcgacacccg ttacagtaaa ttttccttct
     8761 gttagtgttc ctgttttgtc cagcatgatc acatctagat cattggcttg ctccagtgca
     8821 ttgcggtttt tcaaaagtaa tccgttttta gctgctattg atgtggaacg agcaacaact
     8881 aatgggatag ctaaacctag agcatgagga caggcgatga taaagactgt taccatacgt
     8941 tctaatgcat taggaagatc tgtcaagaac aaccaggcga tgaatgccag gatacccaca
     9001 attaatgcga cataaaacag ccattttgcc actttgtcag ataatgactc tagcttggat
     9061 ttttctcctt gtgcttttcg caccatttcc ataaccttcg ctagataacc attttcacca
     9121 gttccagtta cttcgatttc gatcgttcca tccccattga tagaaccgcc aatcactttg
     9181 tcattggctt gttttttgat tcctcttgat tcgccagtca cagctgattc gtcaacaatt
     9241 gtttcacctt tcaagattac accatctgtt ggcattttat caccagaccg gacgatcaaa
     9301 cgatcacctt cgtttacttc tttcaatgaa actgtctctt cttttccatc tttcgtcaag
     9361 cgtttcactg attctggcag taattctgct agtttttgta aagcattgct ggcattcgac
     9421 acagcattca tttcaatcca gtgacctagt agcataataa cgatcaaagt agccaattcc
     9481 cagaagaaat ccatgacgtg tgtatgtgga ttgagcagat ttgcgataaa cgaatagaca
     9541 ctataaatat atgcaactgt aatccccatc gcgatcagtg tcatcatagc aggacttttt
     9601 tgtttcaatt ccatcttagc tccacttaaa aatggctgac cgccatagat aaatagtata
     9661 gttgctaaga tcaatacgag ccaatcagaa ccaggaaacg ttacttgaaa tggaaattcc
     9721 attcccatca ttggtgaaaa aagaatgatt gggatagcta ggataagcga tagccaaaat
     9781 ttttgtttga aattccccat gtgcatggaa tgatccatgc cgccgtgttc gtgatccatg
     9841 tgcatcgaat ggtccatttg tttgctgttt gctatatctc catggttatg tcccatgtgc
     9901 atggaatgat ccatctgact atggtcatgt ttcatctcca tctgtttttc atgagattct
     9961 gtactttctt tgtttactac ttctttttta tgctcatgtt ttttctccat atcgttccca
    10021 ttcattttta ctccatccct tctcttactt tttcatgtgt cgatttaagc acagggagtt
    10081 caacaataca ctgacggaac taaacgccat cgctcctcct gcaatgattg gattcaaaaa
    10141 gcctagtgcg gcaaaaggaa taccaatcgt attatagaca aaagcccaga aaagattttg
    10201 tttgattttt ctcaatgtca acctagacaa ctcgattgtt tgtgcaatgg atgccaaact
    10261 gctattcatc agcgtcacat cggctgtttc catcgcaatg tccgttccac ttcccattgc
    10321 cataccgaca tcagctaatg ctaaggccgg tgcatcattg attccatcgc ctgccatacc
    10381 gacttttttg ccatctttct gaagtttttc tacgtaggct gctttatcct ctggaagtac
    10441 ttcagcaaag atatgctttg gatcaatacc aacttgttta ccaatcgttt cggctgccag
    10501 tttgttgtct cctgtcagca taaaaacatc taatccttta ttctggagct gcttgattgc
    10561 ttgtttgact tctagtttga tttgatcagc cacggcaatc aacccgatca cttgcttttc
    10621 atcagctaaa aacatgactg tttttccctg ctgttctaat tctaacgcgt gttcttggta
    10681 ttcatcaaat gataggttca attcaattaa tcgtttacgt gtacccgcaa aatagcgtac
    10741 tccgttaatc gtaccgctga ttccagcacc tgggtgggct gtaaaatcaa tcatatcgta
    10801 tgttgctgct tcttgcagtt ttccgtattc cacgattgct ttacctagtg gatgttcaga
    10861 agcttgttct aaagtataaa aaatagatag gacttcttta gaaccgacta gatctgtaac
    10921 tttgggcttt ccttctgtaa tcgttccagt tttatccaaa acaatgctgt ctaagtgagc
    10981 agctgcttct aatgcttctc cgcctttaat taaaattcca tttcgtgcac caacacccgt
    11041 tcctaccata atagctgttg gagtcgctaa acctaatgca catggacacg caatgactaa
    11101 tacagagaca ctatggatca atgcgagttg ccaatcacca gtgatcaaac ctgtagctat
    11161 gagcgtgata aacgcgatga ctaatacgat cggaacaaaa atccctgaga tccgatctgc
    11221 gattttttgg attggggctt tgctcccttg agcatcttcc accatttgaa tgatttgcgc
    11281 taatactgtc tgcttgccga cttgtgtaac ttccatgtga agtagaccat tcgtattgat
    11341 tgtcccgcca aagagcgtgt catccggatt cttttctaca ggaagatttt caccagtaag
    11401 catactttca tctatcgcac tgcttcctga aataattcga ccatctgctg gaatctgctc
    11461 accaggacgg atgactagtt gatcaccaac gactacttct tcaatcgcta acgtttcttc
    11521 tttcccgttt cttatcactt gggcggtttt tgtttgaaga gacatcaatt gtttgattgc
    11581 attacctgtt ttcgtttttg ctgtatgttc caaatatttg ccgagcaaaa tcagcgtgat
    11641 gatcatactg ctgctttcaa aataaagatc ttgggggtgt ccacggaaaa atccgttgta
    11701 aatgctcaat gcaaaggcag ccgatgttcc aattgctacc aatacatcca tatttggtgc
    11761 tttcgtcttg attgcatggt acgcaccttt ataaaaacga gcaccgacat aaaattggac
    11821 aggagcagac agcactaact ggacgattgg aaaatgaaaa aagtgaacaa tcgccgcatg
    11881 gcttcctaac atcatagcaa tcattgacaa catcaacggc aaagtcaaaa ccgtactcaa
    11941 tataagatcg aatagcattt ttttcaaata cgcttgcttt tcttctgcaa ttttttgttt
    12001 gtgggcttca tcgtacaaaa ttgcaccata gccgatgttt tctacgcttt ggattaattt
    12061 ttcagtggtc gtcccttcat atttgacact tgctttttca gtggccaagt tgactgtggc
    12121 attgaccaca ccaggttgtc cgttcagctc tttttcgatc ctagcagaac aatttgcaca
    12181 agtcatacct gtaatgacga acgtatccat tttggtttct gctgttgcca tcaaatcacc
    12241 tctgctttat aacccaatcc attgattgct tgacaaatct tttcggattg aacttttgtt
    12301 tcatcaaact tgaccgtacc gtttgctttt ttcaaattta ctttcacttt ttgcacgccg
    12361 tcaagtgcgc ttactgtttc ttctactctt gctgcacaat gattgcagct cataccttcg
    12421 attgaaaatt gttgtttcat tttttcttcc tcctattgat gttgtttaca ttcacattga
    12481 cctgggatac agttacattc gatcgtatct actggttgct tttgttccag ttgtttcaaa
    12541 atctcattga tatcttcttg agtcaaagtg gcttcttcta ctagatccgc aatcgtctct
    12601 ccaatccttt ttgcacagat atgggaaaaa agattttccg tcgcactgcg aactgtttcc
    12661 atctccgaga cagctggata ataaatgaat ttttttcctt cttgttctgt ccagatcact
    12721 tcttttttta ccaaacgacc cagcaaagtt ttgatcgttg ctggtttcca atccatcgaa
    12781 tcagctaaga tctgcgtgat ttcttgggct gtcgtctgtc caagtgtcca aataacacgc
    12841 atgatctccc actctgaatc actgatcttt actggcgttt tgactttttc catttatttc
    12901 acacctttcg tttacaaatg taatcattat cttatgaata cagattacac ttgtaaacaa
    12961 aaactgtcaa ttatttttgc tctctatttt caatttagtt aaaaattctt gtttctctcg
    13021 tcttgtttta aggatgatca gtttatttct tgcattagct acttggatca gctgctgtat
    13081 tttcggttca ttgttttctt caaaatgcca gataaaagat aaaaattcta agtattccct
    13141 gtttagtttc tccgaaaatg attcaggcat atctggccga gattttttgt ttatctttct
    13201 ttttagagaa cgccaaacta ctcggtagat ggcgagataa cgcggcactt taagccaaat
    13261 gatcttatct gcttctttaa tcctttcctc taatgtagaa gtataatttc cttcaacgat
    13321 ccatgacgga ttagatacca taaacaattg ctgttcttgc aaaaaccact tttttgcctg
    13381 aatactgtag tctgttgtat gccataaaag gtctagcggc agtagcggaa agtcggtgat
    13441 ttctttgatc ttccttgcga gggtcgattt tcccgaccca ctttgtccaa tcacgatgat
    13501 cttcattctt ctccctcact tctctttgga atttggttcg atcataccac ttataacgcc
    13561 gcaaaaaaac aagggactgt gacaaaagtc gtgtcaaagt cccttgtttc gaata
//
LOCUS       DACCYH010000078        13347 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.081, whole genome shotgun sequence.
ACCESSION   DACCYH010000078 DACCYH010000000
VERSION     DACCYH010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.081"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(7..549)
                     /locus_tag="IVY85_002184"
     CDS             complement(7..549)
                     /locus_tag="IVY85_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP8045188.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            684..1073
                     /locus_tag="IVY85_002185"
     CDS             684..1073
                     /locus_tag="IVY85_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045189.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1077..1841
                     /locus_tag="IVY85_002186"
     CDS             1077..1841
                     /locus_tag="IVY85_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8045190.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIQLMEEASTFLAKNLIKKYK"
     gene            1970..2917
                     /locus_tag="IVY85_002187"
     CDS             1970..2917
                     /locus_tag="IVY85_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8045191.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3047..3787
                     /locus_tag="IVY85_002188"
     CDS             3047..3787
                     /locus_tag="IVY85_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045192.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4078..4746)
                     /locus_tag="IVY85_002189"
     CDS             complement(4078..4746)
                     /locus_tag="IVY85_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8045193.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            4880..5167
                     /locus_tag="IVY85_002190"
     CDS             4880..5167
                     /locus_tag="IVY85_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045194.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            5583..7121
                     /locus_tag="IVY85_002191"
     CDS             5583..7121
                     /locus_tag="IVY85_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP8045195.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            7118..8083
                     /locus_tag="IVY85_002192"
     CDS             7118..8083
                     /locus_tag="IVY85_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8045196.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            8070..9392
                     /locus_tag="IVY85_002193"
     CDS             8070..9392
                     /locus_tag="IVY85_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8045197.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      9455..9559
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9633..10376
                     /locus_tag="IVY85_002194"
     CDS             9633..10376
                     /locus_tag="IVY85_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8045198.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            10391..11383
                     /locus_tag="IVY85_002195"
     CDS             10391..11383
                     /locus_tag="IVY85_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8045199.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            11395..12132
                     /locus_tag="IVY85_002196"
     CDS             11395..12132
                     /locus_tag="IVY85_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8045200.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            12122..12844
                     /locus_tag="IVY85_002197"
     CDS             12122..12844
                     /locus_tag="IVY85_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8045201.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(12928..>13347)
                     /locus_tag="IVY85_002198"
     CDS             complement(12928..>13347)
                     /locus_tag="IVY85_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP8045202.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         4392 a         2289 c         2781 g         3885 t
ORIGIN      
        1 tgtgagttat tttccttgtc gttttctcac ttttttagtc cagaaaccac ctgaaatata
       61 ttttggctca taagaaatga taaaagcacg ctcttcgatt tctttgatca ggcgatacag
      121 ttcacgttcg ctttttctag gagaaagtat ttcgagaact agccgctcac cttccagccc
      181 cattgcagaa gtctgtgtca caccataacc gtgttctctc agtatacccg gcaaactttt
      241 atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg atgtatccta atgccaattt
      301 gtcttcaatc aaaattccga cactgatacc tactgcgtaa cctaacgcat agacaaataa
      361 attcagtgga ttatctaaac gatctaaaac aagggataag ccgacgatat aaatcgtgat
      421 ctcgaccata ctgacaagtg gagcaatcag tcgatagcct ttcattgtca acatgaaacg
      481 taacgtgttc agagtgatat aagcaaaatt aatgaggaag atcatcccca acatttttgg
      541 atcaatcatt tgtaaaactc ctttcattta tataacaaca atatcaaaaa aaatcgtaag
      601 tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata aaatgattat aatagaatta
      661 taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg tgtgttttgt gccagccacg
      721 tgctaagtct ccttctcagt atatacttag gtgtctattc gattcaacag ccaattgagg
      781 tagagaacat tgtttttcag atgtcgctga cagatggttt aattttaagc gtttttttct
      841 ttttgatact gtttattggt aatattttcg gtagcgttgc cagttttttg aatttttcta
      901 tttatccact cctttcgcta gcattaggag tggtgggact ttgttctctg tgcttatttc
      961 ccgagccatt cagtcctgca tttatattat ttggtacact gaacctgttc cagacaactg
     1021 ttggagcatg gttgctatgg cgatcaggaa atttaatgaa aataggtgag taacaattga
     1081 atccaattat attgaaacgt tctgtccatc atattccagt gttagaagtt gttctgaaag
     1141 aattaagaga ggaacgtatt cccgttgtca tctattatca cggatggcag acaagtaagg
     1201 agttagtttt gacgcaagga agaaaaattg cgcagcaggg cattcgtgtg atcctgccag
     1261 atgcgatgaa tcatggggag cgcaaacagc cggtttcttc tattccatcc tatacatttt
     1321 gggatagtat ctacggaaat ctgtttgaat ttgacacact tatcaagcat ttagaaaaaa
     1381 gaggtcttct acatgatcag cttgcagttg gcggggtttc gatgggggga atgacaacat
     1441 gcgccttgtt ggctaaacat cctcatatag tagcgggcgt gtgtttgatg ggttcccctg
     1501 cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc ggaatataat tatgtcgtac
     1561 caaaagacta ttttgatttg atttcatggg ttaagagcta tgatttgtca ttgcagccgg
     1621 aaaagttagc tggacgtcct ctgttctttt ggcatggaca agctgacgaa aaaatccctt
     1681 atatacaaac ggaacgattt gtagaaaaaa atcggcataa gtcttacgga aaagaaattg
     1741 tttttcgttc ctcaaaaaca cagcgacata tggtacaaat acaactaatg gaagaagctt
     1801 ccactttttt agcaaaaaat ttgataaaaa agtataaata attgaaacgg aaaatcactc
     1861 atgacgatag aggattttcc gttttttatt ttgggctata ataaactctg ttaggaaaat
     1921 ttggtataat tgatagtaag aatgacaaca tgttagaaag gaagagagca tgcccacttt
     1981 agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg gtctctcgag tcatcaatca
     2041 tccggaacag gtgactgacg aattgaaaga gttagttttc tcagcaatgg ctgaactgga
     2101 ttaccgtcca aatattgctg caaaagcact tgtcacgaat cggtcgcaga tcatcaaatt
     2161 atttatactg gaagaaatcg acacaactga gccctattac atgaatttgc tgatgggaat
     2221 cgctaaacgg atcggtaaag cgcattattc tctccaatta gttacaaatg atgccttcga
     2281 cgtcggttct tgtgatggat acatcatcac aggtgtcaga aaaaaagact tcgattggat
     2341 cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat tctgaaggat atgattatgt
     2401 agacagcgat aatgaatatg gtacagcaaa agcaacgcaa tatgccttag aaagagatta
     2461 tcaaacgatt atttatatcg gtatcgaagt ggacgagcct tttgaaattt caagggaaac
     2521 tggttattta tctgtgatga aggaagttga tcttcctagt aaaatcttgc ggtttccaaa
     2581 tcattcgaca gaagtagaaa gatatatgac agaaaactgg gcagatttta cagataaaac
     2641 ttgttttgta tgtagttcag atcggctagc actaggagca gtaagaggga ttataaatgc
     2701 tggaggacac ttgcccgaag aatttggtgt gattggattt gatggggtat ttttagacca
     2761 aatcagttca cctaaattga caacgatcaa gcaagatatt atacgtatgg gtgaagtatg
     2821 tggagaaatg ttgttaaaaa aaatagaaga aaaaggggcg agtcaaggtt atcgtcgttt
     2881 ttttcctgaa ctagttatca gagagacaac gaattaagtc ttattactat atatatattt
     2941 atgaaattca tcgctatttt cttaaaataa tcagcgcatt gctactgaaa acaatgaatc
     3001 tgtgctatta ttgagaaaag ttccattttt gagaaggatg gtttgaatga aagaaatcag
     3061 acgaaaacat ttccgttttc ctgcgtacga tgatgagtta ggcgtaaaat taaccaacga
     3121 caagaagcga gtattatttg acggacaaga agatcttttg actgaacaga atcagtcccc
     3181 gtttgaacag atgaaagaat ttcatttcac gaacacgaac accgagaaaa tccgtcgcaa
     3241 gcgtcgtgca acaaatggat tgaagtcagc tccgcgtaag gaaaatttgg aaaagcataa
     3301 aggaaaatta ccggattata ccggccatta ccaatcagaa atcacatcga caggacgaaa
     3361 aaaacttttt ggtgattcct ctcgttctgt cagacaaagt gaaacaagga cagagtccaa
     3421 gcatactaca cgaaacgcat ctgttgacac gaaggtgcgg gagcgttctt attttgttcc
     3481 taaatatgta cccgcatcga ttatcccaga ccctagagaa agcaaaattt ctgaggatca
     3541 tttaatcgaa tcgatgaaaa aaacggatga ctcttatctg ttgttcgata cagaaccgac
     3601 gccgtatcaa gaaaaaagaa atggtgagcc gtctgtacga acattcaatc gaacaaaaac
     3661 gttagaggaa acaaagcagt cagaagaaaa aaaatcgaaa aaaagccatg gtatcttaga
     3721 acgctcatta caaggattga tcgatgatca aggaaactct ttgggagaaa atagctattt
     3781 taaataagtg atatacaaat ttatattcgg ctacagttgg aaaggaaacg ctgcggcgtt
     3841 tccttatttt atactctatc cgaaagttta tatcgtatcc tcaaattttt ttgagtttaa
     3901 gcgaattttt tctcccaata agtaagaaaa taatggcaaa ataaaaaaac aaactatttt
     3961 cgcagatcgt ttgcaaaaat agtttatttt tttataaaga agattttact gaataatatc
     4021 attttagttt acataatgat atttctgtaa attaaataaa gtagtgaaaa ataagaatca
     4081 gtgctctaac tgttcaagag gatcaagtgg ttcaaatgtt tctgtagtct gttttgcttc
     4141 caattggggc aaaacagatc caatttccag atacttcact ttattcaatg tataagatag
     4201 aatagaacag ctaagtaatg tatacagaaa atgtgtgaga tccaaataaa gcaaggagac
     4261 aactaaaata ccaaaagtaa tcaagaaaag acttttgcca atcttaatat gaaaatattt
     4321 ttcaatggct tgataaatga tatctgttcc gcctgtgctt ccgccgaatc gaaagacgag
     4381 accgcaacca atgccagaaa gaatacctgt catcaaacta ttgatcagta gactatctac
     4441 agcaaaatgt atatgaaggc tctcccataa atgtaaaaag aaaactagcg caaatgttcc
     4501 gacgatcgtc cggagaaaag cgtgttttcc aaaaagaaac aatgagataa gcaataaagg
     4561 taaattaaaa agcagtgaag tgacagaagg agcgaatcca aaaagattat gcaaaagcaa
     4621 agcaatacca gtaaccccac catctgccag cttagatgga atattgattc ctgtcacagc
     4681 taatccgtag attcctgcgc ctaaaatgat tccgcacaat tctgtaaata aatttaattt
     4741 tttcatttct agcctctttt cttttgaaaa ttttcagcaa cttctttatt atactgactt
     4801 atactcttac ttcaagagga aaatgaaaaa aaattatttt tggctgtttc tttttattta
     4861 tcaaataagg agtggaaaaa tgcgagcagc agacttattg acaattctta caaaatcacc
     4921 caaaaaaaca gagtatcaag gagttttttt ggctcaagaa caaaaactgg tgcctgtgac
     4981 aatggtccag ataaataaag caaatcaatt tatcttttta tttgaacacg gcaaagcacc
     5041 acttgctatg aaagacatct ttcttttttt gatgaagaac cgtgagaaag ctgtttatta
     5101 tcagtcaaaa gaagaactca ttcctcttta tggagtgaaa gagatagaaa ataaagtggt
     5161 gatataagta aaaataagaa aacaagacgt ttatgatgtc aaaacaacca ttttttacat
     5221 ttttggcaaa aaagtgtcat gatacagtat aatattcgtt gaacaggcca ataaaggtgt
     5281 tctcccccac gagatttcta ctttattggc tgtttctttt tattaaaata aaagttgtgt
     5341 gtgtaaaaaa gatatagctg attccaagtg acaatttcgt atagattttt atggtattcg
     5401 tagtgaaaaa acgagaacca aaaagttgat ccgactttgt tgcttggatt ctttttttgt
     5461 cagcatattt agaggtattt ttttcttttt tttgttcgtg ctgtaaggtt aaggcaagac
     5521 aagttagttg ggaaaaagga aagaaatgtt tatacttaaa gaataacaga aaggaaggtc
     5581 gcatgaataa ttttatacaa acattaagtg aaagaaaagg cgagttgctg gaagctgcat
     5641 ttcaacatct gtccatttct ctcatttctt tgctgattgc agcactgatt gctattcctt
     5701 tagcgatttg ggcagccaat cataaaaaaa tggcagaagt cttattgcag atcacgggtg
     5761 tgctccaaac gattccttca cttgccttat taggtttact tatcccattt gtagggatcg
     5821 gaactgttcc agcattgatt gcacttgtta tttacgcttt gcttccgatt ttccaaaaca
     5881 cgtatattgg tctagctgaa attgacccgt ctattgaaga agcagcggta gcatttggta
     5941 tgtccagaat gagacgcttg ctgaaagtag agcttcccat cgcattacct gtcattatct
     6001 caggtatccg aactgcctta gttttaatca tcggtacggc tactttggca gcattgatcg
     6061 gagcaggtgg attagggaca tttattttgt taggtattga ccgaaataca cctgttttga
     6121 cattgattgg tgcgatcagt tctgccttgc ttgcaattat attcagcgga ctgattcgct
     6181 ggttgcagca taagaaacct cgatattcca tcattacttt agcagcagtg gtgtgtttat
     6241 ttggcggcgt ttctttatat cagtctgcct tgtttaaaaa tgaaaccatc acgattgctg
     6301 gaaaattagg agcggaacct gaaatcctga tccaaatgta taaagaattg attgaagaca
     6361 acacaaaaac agaagttgaa ttaaaaccga attttggcaa gacgagtttt ttgttcaatg
     6421 cactggatca aggtcaaatc gatatctatc ctgagttcac aggtactgtc ctagaaagct
     6481 tggtgaaggt acctgaagca atgaaaagtg ctgatttgac aaaagaaaaa acatatgacg
     6541 aagcagaaga attattgaat aagcaattcg atatgacatt attaaaacca atggcttacc
     6601 aaaatacgta tgccttggca gtcaagagat cttttgcgaa cgaacatcaa cttaaaaaga
     6661 tttccgattt ggcagctatc tctgatcaga tcaaagcggg attcacactg gaatttattg
     6721 atcgttcaga tggatataaa ggaatccaag aagcatatgg cttgaatttt tcaagtgtac
     6781 aaagcatgga gccaagtttg cgttaccaag caatcaataa tggagatgtc aatattgtgg
     6841 atgcctattc cacagatagc cagctcaagg agtacgatct agtgactcta gaagatgatc
     6901 gagggttatt cccagcttat caaggcgcac cgctgatgaa aaaagatttt gctaaagaac
     6961 atcctgaaat cgttaaagca ctgaataaat taagcggaaa aataacagag gatcaaatga
     7021 ttgatatgaa ttaccaagtc aatgtcgaaa agaaaaagcc gacagaagta gcacgaatct
     7081 tcctgaaaaa tgaaggatta gtaaaggagg aagatcagtg actaaattaa tcgagttcaa
     7141 acatgtgcaa aaaaagtatg atggaaagta tgtcatcgat gacctaaatc tggcaatcaa
     7201 aaaaggagaa atctttgttt tggttggtcc ttccggtagt ggaaaaacaa ctactttgaa
     7261 aatgatcaat ggattatcta aaccatcagc aggagacatc tacttcaaag gaaaatcgtt
     7321 aaatgaatat aatctgcaga aaatgcggtg gaatatggga tatgtgctgc agcaaatcgc
     7381 tttgttcccg accatgacag tcaagcaaaa tattgaagtc atccctgaaa tgttaggttg
     7441 ggaaaagcag aaaagagcag accgtgtaga tgaacttctt caaaaagttg gcttatctcc
     7501 tgatatttat cgagatcgta tgcctagaga gttgtctgga ggagaacagc aacggatcgg
     7561 gatcatccga gctattgctg caagcccaga tgtgattttg atggacgagc catttagcgc
     7621 attagatccg atttcaagaa actcattaca agaattggtt ttgtccttac acgaagaatt
     7681 gggcacgacc atcgttttcg ttacgcacaa tatggaagaa gcaatcaagc taggtgatcg
     7741 tattgcattt atgaaggatg gagaaattat ccaatgtgac acaccagagc agttattgat
     7801 gaatcctaaa aacgactacg ttcggcattt ctttgatgaa ccgaaacaga cgaaagagtg
     7861 gcgagtagaa gatttagtcg tcaatggata tttcttaaat gaaataccag aaaatgctcg
     7921 tcaacaagtt tgctttgata cgcctatgaa agaagtatat tcgcttcttt cggtgtatcc
     7981 aagcattgtg atagtagaaa agcaaagaag tattgggtcg ctgacaagta aagaaatctt
     8041 tgctttttta agtcgagagg aggaaggaaa tgaaaacata tgatgcaatc atcatcggtt
     8101 caggtgtgag cggcttaagt gctgcttatg gattgaaaga agcaggtaaa acagtactag
     8161 tggtcgaaga agatctatgg ggcgggactt gtcctaaccg tggatgtgat cctaaaaaag
     8221 tcttgttaag tgcagtagag gcacgtaatc gggtaaaaca attgagtgga aaaggtttta
     8281 acgagatccc aactgccaac tgggaagaac ttcaaaaatt caaacgaaca tttacggatc
     8341 cagtcccaga aagccgaaaa aaacaattag cagaagctga aatcgatcat ctttcaggaa
     8401 ccgctcgttt tttggatgat tcatctattg aagtcaacga agaagtcttc catgcggatt
     8461 acttagtttt ggcaaccggg caaagaccta cgatcttacc agtggaggga aaggaatact
     8521 tgaaaactag tgcagacttt ttatcattgc ctgtactgcc taaagaaatc atttttatcg
     8581 gtggtggtta tattgctttt gagctagcta cgattgccaa tgctgcaggg agcaaagtaa
     8641 ccattgtcca tcataatcaa cggccattaa aagaatttga ggcgtctttg gtagaggaag
     8701 ccgtccatca aatggaagca tctggcattc agtttgcttt tggtgttgaa acccaaaaga
     8761 tcatttcaga aggtactcgc tatcgtttag tcggaaaaga gacggaatta gtagcagata
     8821 tgattttttg tgcgactggg agacagccga atacagaatc tttagcactt gagcaagcga
     8881 atatcgtctt tgataaacac ggaattgcag tcaatgatta tctgcaaacg agcaatccaa
     8941 aaatttttgc gtgtggagat attgtttcaa ggaaaacccc aaaattgaca ccagtcgcca
     9001 cttttgaagg aaattatgta gctaagcgga taacggatgc aacaagcgaa cctatcaaat
     9061 atccgattat acctacgatc gtctacgcca gtcctaaact tgctgaagta ggagttacaa
     9121 aaagtcacgc ctcttcgtca gatcaagtgg tagagatgga tcttaccagt tggttcacct
     9181 atcatcgtgt caatgaacca gttgctaagg ctgaattgac ctttgaccag caaaattatt
     9241 tgattggtgc tgcagtgatt agcgagcaag cagacgaatt gatcgatgat ttgacactcg
     9301 tcatcaatca aaaattgact aaaaaagagt tggatagcta tatcatgggg tatccgacat
     9361 tggcaagtga tctctcctat ttattgaaat aatcagcatg ataactaaag cgcttgttgc
     9421 tttttccttc ttgtgctaca ctttaaaaaa tgaatacctg tttctgggga tgtcgtagac
     9481 gacatgagag taagttgtga acttagaccc attgaacctg tcagttaata ctggtgaagg
     9541 gagaaacata gcagacgctt gaagcgggtt actatgtcgt gcccatttca agcgtttttt
     9601 gttattcata ttagaagaaa ggaggtaaca aaatgaagaa aaatctttat ccagcagtca
     9661 gtatggcatt cattttaatt atttggcaga ttgttgtcat agtttggaaa atacctgcct
     9721 ttattttacc cggacctgct gctgtatttt ctgcattagc ggcagatgca caagtattac
     9781 tttcccattc tgtcacgaca ttaaatgaag cagtgcttgg tttggtaata gctagcgttc
     9841 tttcttttat tactgccttg gcaatggatt attggaagtt tttagaaatg tctttttatc
     9901 cgttactagt gatttcacaa atgttgccaa tcatggtgtt agggccatta cttaccctat
     9961 ggtttggttt cggtatgctt ccaaaagtga tccttgttgt cttgataagc tattttccga
    10021 tcgtagtttc tttttcggat acattgagga aagtatcaaa agagcaaatc atctttttga
    10081 aaacgatggg ggcagataca gggaagattt atcgaatata taaaatacca gcgggtttat
    10141 ccggcttctt ttccggttta aaagtggctg ctacttattg tgtcagcgga gcagtcgttg
    10201 gagaatggct aagcgctcaa gcaggcttag ggtattacat gatacgcgta aaaaacagct
    10261 accagatcga taaagttttt gctgcgatca tttgtgtcat tctattgagt ttattgctga
    10321 atggcgcttg ttcattattg aaaaaagggt attatcgtat gctttatatt ttttaagaga
    10381 aggggatcaa atgaaaaaat tatggctttt attaccgcta ctgttgcttt tatccgcttg
    10441 tggcactgca aaagaaacaa gtagcaaaca agaaataaag gatttgaaaa aagtgacact
    10501 tgttttggat tatgtaccga atactaatca tactggcatc tacctagcaa aagaaaaagg
    10561 ctattataaa gaggctggtt taaatgtcca aatcatcgaa ccaggagata attcgacaag
    10621 tattggtcta gtaggtgcgg acaaagcaca atttggtgta agttatcaag aagatgtcac
    10681 ttatgctcac gcagatggac agaatatacc agtcaaagca attgctacag tgatcaaaca
    10741 taatacttct ggattcgcca ccctatcaga cagtaacatc cattcaccaa aagattttga
    10801 aggaaaaaca tatgcaggtt ggcaaagtcc aagtgaagaa gctgtcttaa aagctgtgat
    10861 ggaaaaagac ggcggggact tttcaaaact gacaatggta ggaagcaatg gagaagggcc
    10921 agaaagctta ggaaaatcct cagatatcca atggtacttt gaaggttggg atatgatcaa
    10981 agcaaaagaa gcgggtattg aactaaacta tatcccattg aaagaattag atgaacggtt
    11041 agattactat actccggtaa ttattacgaa tgatcaactg atcaaaagtg atccagaatt
    11101 ggtacagtct tttatggatg ccacgaaaaa aggctatcag gaagctatta aggatccaaa
    11161 tgacagtgct aaactgttgc aaaaatatgc aaaagaaaat gatcggacgt ttttagaaga
    11221 atcacaagca ttcttgtcaa aaaattatac ggatgatcct gagaattggg gattgatgga
    11281 agaaaaagtt tggcgcaatt atacagcttt tatgcaggaa aacggattga tcaagcaaga
    11341 tgtaccttcg caagaactat tcactaatca gtttataaaa taggagtgat agatatgtca
    11401 atccaaatca gccacttgaa tgtagtcatt gaaaacctga aaatattaga agacatccat
    11461 ttcactatcc ctaaagatac ctttacatcg attgtcgcgc caagcggagc cggtaagtca
    11521 acattgctga aaactttaac aggagtccag cctatgactt ctggaacgat caaagtagac
    11581 gatcaaaaag taactggtct ccatacagca tttagttata tgccgcaaga agatatgtta
    11641 cttccttggc tgagcgtcta tcaaaacgtt acgttatatc aaaaaatcaa tcatctaaca
    11701 atcaatgaag aacaagtaag ggattatctg gatatctttg ggttaaacgg ctatgaacat
    11761 tttttgccgg aacaattatc tggcgggatg aagcagcgaa cggccttgtt acgaacaatc
    11821 atgaatcctt cttcttactt gcttcttgat gaaccatttg gagcgttaga tgctatgacc
    11881 agagggcaga tgcaagactg gatgttgaag ctgcctaaaa aagcaaagcg gactacttta
    11941 cttgtgacgc atgatatcga agaagcaatt tatttatcag acaggattct tgtgctttcc
    12001 gcccgtccgg cacatgtgat tgctgagatt caagtgcctg aaaaacgacg gtcacgtgaa
    12061 tggctactgc agcaatcaga gttgaagcaa acgatttatc agttattggc aggtgaatta
    12121 gatgttaagt gatgctcatt gtcatttaga tgggagtcag agtttagcac tccttcagca
    12181 ggaacacacg attttgacga tcatcaattg tgattcgcca gaagaatgga aggaaaacag
    12241 acagcttgca gcaagtaaga ctcaagcctt aagttacggt atccatcctt ggaaagcaga
    12301 tagctatacg tttgaacaag ttgagccttt tttgaaaaaa gcaaggatca tcggtgaaat
    12361 agggttagat aatatttgga cgaatgtacc gatgactaca caaaaaaaag tatttgagcg
    12421 acagcttgct tttgcagcca taaatgagaa acctgttgta ctgcacacaa aaggatgcga
    12481 aaaagagatc ttaaattata tccaagctta ccctaatcac tatttgatcc attggtattc
    12541 ttctttggac tatcaacaag actatattga tttaggctgt tatttcagca ttggtcttga
    12601 tttgaaaaag aatccagcag tctggcaact agctaaagca gtcccaatcg accacttact
    12661 tattgaaacg gatggactac atgcagcaga gtgggcttta ggggggattt tttcagaaaa
    12721 agattatgtc tcactgcttg aagaacagtt aaagacaatc gctgagatca aagagctatc
    12781 agttgaagaa gttaaaaaaa atacttatca aaacttggaa cgtttcatta gattaggtaa
    12841 ataaaaaaga gatgtgacac aactagtgtc gcacctcttt ttggctttct ttgtataaaa
    12901 gtaaactgtt tgattaataa atgaatctta gtaccaaccg tttgcttccc agaatgcttt
    12961 cgccgcttcc caagaaccat aacgtgaagt aacatattgt tctgctactc tttcttggtt
    13021 tgcagcagag tagtcaccat ttaagtaaga agaatctaat tggtaacgtc cgatgtaacg
    13081 tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt gcgatccatt cttttgcaga
    13141 gcttgtgctt gctggagtag cagcagtttc tgttacagca ggagcaggtt gtgtttcagc
    13201 aacaggcgct gcttgttttt ccgtttgaac cgtttcttgt acaacaggtt gttcagcttg
    13261 gactggtgct tcttgaacgg ctgcttgagg ttcagcagct gcttgtgcag agccttctgt
    13321 tgggattgtt aattgttgtc ctgagta
//
LOCUS       DACCYH010000079        13035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.083, whole genome shotgun sequence.
ACCESSION   DACCYH010000079 DACCYH010000000
VERSION     DACCYH010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.083"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(80..152)
                     /locus_tag="IVY85_002199"
     tRNA            complement(80..152)
                     /locus_tag="IVY85_002199"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(117..119),aa:Lys,seq:ctt)
     gene            533..1102
                     /locus_tag="IVY85_002200"
     CDS             533..1102
                     /locus_tag="IVY85_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP8045203.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1089..1949
                     /locus_tag="IVY85_002201"
     CDS             1089..1949
                     /locus_tag="IVY85_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8045204.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2139..2843
                     /locus_tag="IVY85_002202"
     CDS             2139..2843
                     /locus_tag="IVY85_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8045205.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2848..4683
                     /gene="walK"
                     /locus_tag="IVY85_002203"
     CDS             2848..4683
                     /gene="walK"
                     /locus_tag="IVY85_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP8045206.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4680..5996
                     /locus_tag="IVY85_002204"
     CDS             4680..5996
                     /locus_tag="IVY85_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045207.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5997..6866
                     /locus_tag="IVY85_002205"
     CDS             5997..6866
                     /locus_tag="IVY85_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045208.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6921..7730
                     /locus_tag="IVY85_002206"
     CDS             6921..7730
                     /locus_tag="IVY85_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8045209.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7833..8462)
                     /locus_tag="IVY85_002207"
     CDS             complement(7833..8462)
                     /locus_tag="IVY85_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAP8045210.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8632..9924
                     /locus_tag="IVY85_002208"
     CDS             8632..9924
                     /locus_tag="IVY85_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8045211.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10000..10662)
                     /locus_tag="IVY85_002209"
     CDS             complement(10000..10662)
                     /locus_tag="IVY85_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8045212.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10887..11171
                     /locus_tag="IVY85_002210"
     CDS             10887..11171
                     /locus_tag="IVY85_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP8045213.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11222..12847
                     /gene="groL"
                     /locus_tag="IVY85_002211"
     CDS             11222..12847
                     /gene="groL"
                     /locus_tag="IVY85_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP8045214.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4335 a         2252 c         2820 g         3628 t
ORIGIN      
        1 aaataaaaaa agaacagcgt aaatgttttc gaaatgcttt cgcaccaaca tttacctgtt
       61 ccgtgggttt ggcacccaat gggccgtgag gggctcgaac ccgcgacccg ctgattaaga
      121 gtcagctgct ctaccaactg agctaacggc ccgtacaaaa agtacttaat aatagtagca
      181 ctaagaattt taaaatgcaa gactacattt aaagaaaatc agaaaaatct ttacttcctt
      241 ataggaaatc attcatagat tattcacatt tgctcgctat tatataagca taccaacaaa
      301 caaagttctt gcaagagaac aaaccctaac acttttttca ttttttactc ctttcccgcc
      361 gtctccccgg cgggtctcct gttcttaacg ataaaagaca agaaggctta gccttcttgt
      421 tttttgtttt catggaatcc cgctaatcaa atcacctagt taaagcaact agattgaatg
      481 tttatcataa gtctgatatc atgtttatga aatgaaaaaa ggagcggtta tgatggaaaa
      541 aatgcgtcag caattaatgg agtgggggaa ggaactagaa acattccgtc tgcctcactg
      601 ggaagaactc cctgacttag aactttatat ggaccaagtc agaacgttag ttgatcgtta
      661 tctttcacct gtgatacaag gagaaaagca tccattgctt acctcttcta tggtaaacaa
      721 ttacgttaaa ctaggtttga taccagcacc agttaaaaaa agatataaca aagaacacgt
      781 agcattttta cttgcaatca ctacgttgaa acaagtattg actattcctg aaatcaaaga
      841 agggatattg ttccaaggaa aaacagtagg aattcgtgaa gcctataatc tattttgcga
      901 tgaacaagaa gcagctgtgt ggatggtcag ccaattagca caaggtaaat ctcatccgca
      961 aaaattcgac caggcaaccc ctgtagaata tattgctgta agagcggcaa ctttatcgtt
     1021 tgcaatgaaa cttcttgcgg aaaaaacgat tgttctagaa accgaatatt taaaagagga
     1081 gaaaacaaat gaaaaacaat aaaatcgcaa ttttagtaga ttcaggtacg gatgtgccag
     1141 aagaattgat aaaaaaatac gagatgtata tgatcccatt gaaaattatt tataaagatc
     1201 aggtatatac agataaaatt gacattacct ctgaagagat ctatcaacgt ttgccagagg
     1261 aaatccctgg tacatctttg ccggatggtg agtcgatcac agaaatcttt gagcaaatca
     1321 aaaaagacgg ttatgaaaag gtacttgcgg taacaatttc cagtggattg agcgggacat
     1381 ataatgtggt tcgtttaatt gcacaacagc aagaagaatt ggaagcatat gtactagata
     1441 cgaaaaatat tggtattggt gcaggttttt ctgcgatcca agcagcaaaa tggctggaag
     1501 aaggagtaga atgggatcaa ctgatcagta atctgaatga gttagtcgaa agaacaaaag
     1561 ttttctttaa tgttgcgaca ttggaatatc ttcaaaaagg tggacggatc ggtttagttg
     1621 cttctattgt aggaaccgct ttaaagctta acccaatcat ctcatgtaat gaagaaggga
     1681 tttattacac agtaggaaaa gctaggggac gtaaaaaaag tttggataaa acggttgctt
     1741 acgtcaaaga aaaaatcggt aatgcaaagg tcttcaactt agctgttgcc catggggatg
     1801 cgaaagaaga agcaattgaa atggaagcga gacttaaaag agagttccca caagcaaacc
     1861 aaatctactt tggccagatt tctccggcat tagtagtcca cactggtccg ggattattgg
     1921 gtgtcggtgt acaagtttta gaaggataaa tcaaaaaaag gaaacactga ggtgaaaatc
     1981 agtgttttcc ttttttattg aagttagaac aaaaaataat aaagtatttt gcactttcat
     2041 tttaattttc tcttttgttt tataggcaaa gatgaaacaa aagcagaaat acggtataat
     2101 gagaaggtat gacaataata tataagaggt ggctctttat gaaaaaagtt ttagttgtcg
     2161 atgatgagaa gccgatttcg gatatcgtca aattcaattt agccaaagaa ggctatgatg
     2221 tttatactgc atatgatggg gaagaagcct tggaaaaagt tgctgaggta gaacctgatt
     2281 tgattttact tgatttgatg ctacctaaaa tggacggctt agaagttgct cgtgaggtac
     2341 gcaaaactta tgatatgccg atcattatgg taacagccaa agattctgaa attgataaag
     2401 tcctaggctt agagttaggc gccgatgatt acgtgaccaa gccattttcc aaccgagaat
     2461 tagttgcacg tgtcaaagca aatcttcgtc gcggtgccac ggctgcgaag gaaccagaag
     2521 aggcagcacc tgcggaattg acgattggtg atttgacgat ccatccagaa gcttacatgg
     2581 tgacgaaacg aggagaaaca atcgagttaa cacatcgtga attcgaacta ctcttttact
     2641 tggcgaagca ccttggacaa gtgatgacaa gagaacattt attacaaacg gtttggggtt
     2701 atgattattt tggagatgta cgaacagtgg atgtaactgt acgtcgtttg cgtgaaaaga
     2761 tcgaagataa tccaagccat ccaaattatt tagtgacacg tcgtggtgtc ggttactatt
     2821 tgcgtaaccc agaacaggag taattggatg aaaagaaaag tccgcttttt tcgatcagtc
     2881 aattttaaga tcgctattac ctttattctg attttattga tctcgatcga aattattggt
     2941 gcctacttta tacgtggttt agaacgttcc acgatcaata catttattaa agatatgaat
     3001 caaacggtgg aatcattagc aacaacaatc agcccggaat tgaatcgaaa agataatgca
     3061 gatgatgaag aggtaaatgc gaatatcaaa agatttattg aaaacagtgc gactagcgat
     3121 atcatcgaaa tacgggtcgt tgacgaaaaa gggatcatcc gaggaacaac cgatgtcaat
     3181 gaacaaagcg ctgtgggaaa gaaaaatgat tatgttgata tcaatgattt cacgactaaa
     3241 cgttatgttg ctttagataa cgacgataaa cgggtgaata tcaatgttca accgatcctg
     3301 tccccgaccg gcgatgccgt tatcggggct ctttacgtca aaagtaatat cgaacaaaaa
     3361 tattcggaaa tcaataacac agcagtcatt ttctttactg cctcactaat tgcggcattt
     3421 atttcaatga tcgtatctgt tttagttgct cgttcgatta cacagccgat cggtgaaatg
     3481 agagaacaag ccattcgaat cgctagagga gactacagtc ggaaagtcaa agtgcatgga
     3541 caagatgaac ttggacagct ggctgatacc ttcaatcagt tagctgaacg tattgaagaa
     3601 gctcaagata caatggaagc tgaacgcaat cgtttggata gcgtgctatc ccatatgaca
     3661 gacggcgtca ttgcgacaga tcgcaggggt aaagtcatta cgatcaatga tatggctgtt
     3721 tccttgctag atatcaaaaa cgaggaagca atcggacaat cgattttgac acttttagat
     3781 atcgaagaag aatatacgtt gcggaaatta ttagaagatc caaacgaaat ggtacttgac
     3841 cgttcaacca gtacattgga aagtgatcag atgatcttgc gagtagactt ttctatgatc
     3901 cgtcgcgaat caggttttat cagcggatta gtagctgtct tgcatgatgt gacagaacaa
     3961 gaaaaaacag aacgggaacg tcgtgaattc gtctctaatg tttcccatga acttcgtaca
     4021 ccgttgacaa gtatgcgtag ttatatcgaa gcattgagcg aaggtgcgtg gaaagacgag
     4081 gaagtagcac caaatttttt gaaggtaacg ctagatgaaa cagaccgaat gattcgaatg
     4141 atcaatgacc tcttaaatct atctcgcatg gatacaggta atacccagct tcaattagaa
     4201 tatgtcaatt tcaacgagtt agtgaatttt gttcttgacc ggtttgatat gatggtggga
     4261 aatcaggaaa aaaattataa gatccgtcgt gaatttacgc aaagagatct ttgggtcgaa
     4321 gtagatacag ataaaatcat ccaagtagtc gataatatca tgaataacgc gatcaagtat
     4381 tcaccagatg ggggaacgat tacttgtcgc ttattagaaa cgcataataa cgtggtttta
     4441 agtatcaccg atcaaggatt ggggattcct aaaaaagatc tgaaccgagt gttcgagcgg
     4501 ttctatcgag tagataaagc acgtgcaaga gcacaaggtg ggacaggact tggattagct
     4561 atttctagag aagtgattaa ggcgcatcga ggcgctattt gggcagagag caaggaagga
     4621 aaagggtcga ctttttatat ctcgctgcca tatgaacctt atgaggagga atggtgggaa
     4681 tgagaatctc tgaaaaagtc gttcgtatag gactcatttt gatggttgcg ttaagcatat
     4741 acttctctta tgcaatctgg ctgagcccag caggaaaaag ttcaatcaac gtagatgaga
     4801 caaacactca aatgatcgag tctcaaagca atcgaaaaac ttccgaagtc tttcttccgc
     4861 ttcatgttac atggcaccat tcgggaatga tccaagaaac caacagtgaa aatctcgtgt
     4921 accgattgca attgatcatc gataaagcaa gatttggaaa gctttctgaa gtagtttcag
     4981 gagacaaatc acagtttgaa caggtgaaaa atctgaactc tggttttgag ttggcctata
     5041 atgcgccttt tttgctaaga gaatacaagg aagcatttga tttagattta gatgtgtcga
     5101 acttggcaaa agaaagcgaa aatatgtatt ttactagaat acaattcgat caaggaaata
     5161 aaaaaatccg atttttgaat tattcgaacc atctcgtata tgaagcaaat atttcgttga
     5221 attggacaga tatcaataaa gaattaacag caaaagacac aaaatggaca gaaatgacaa
     5281 cagaaccatc gacgatcgac acgcaatatg ctacgaaaca ttcgatcaaa ctgaaaaagt
     5341 atagttatat cctgtcacag caaccctata cactttttag aaatgctttt ttccagaaac
     5401 cagatagcgt gaaaaataat gatgaaagca atgaattgat tttttacggt gggaatgaaa
     5461 caatgtctat ccattcggaa acgcaagtag ttgattttcg tggagagctg ccagagaaaa
     5521 aagaaaatgc tagtgtattt tctgaaagtt tcccctatat tagcaaatta ggcagttctt
     5581 tagggaacct cagatatttt gaccgcagtg acaatacgat cgattatcgg atattcgtgg
     5641 aaggcttccc ggtttttggg acagacagca gaggacagat tggtgtggaa gttggcggtg
     5701 aaacgaatgg ttctgtgcaa gtgaatatcc aaaccagttt gaatacgatt caagtgccga
     5761 taccttcaga cgaagaagta gaacttgcaa gtacgaatga aatcatccag cagttaaaag
     5821 ctaagggagc agatagcaac aagattggtt cagtgatcgt tggctataca tggcaaaatg
     5881 tcaaagaaac gaatcgtgtc gttgatctct tacctgaatg gtatgtgaaa tataaagata
     5941 actggtattc agctaacgac cttctggaaa aattacctgg attggaggcg aattaattgg
     6001 atttcaaaaa aatcgaatgg atatttttcg tcgctttttt agggttgaat gtgtttttat
     6061 tcagcagtta tcatgacgcg gtataccaag aaaacaatgt cattcgctct aatcagactg
     6121 aatcaatcca acaacgtctg gctagcgacg atatcactta cacggggaag atttcgtctg
     6181 agaacaagca aggctattat cttagcgcgg agcagaccaa tcttagcact tcattagcga
     6241 attatcgcaa ggctggtaaa agcggcttgc tggcaagtgg tgtgttttta gacggtgatg
     6301 ttttgacgaa atctttatcc agttcagatg ctgaaaagaa cgtgatcgat ccagataaaa
     6361 ttagtagttc cttggaagat tttttatcta acaaagacaa tattttattc ggtaatgatt
     6421 atgtgtacac gaaaaacttt tctcatttag aagaagaacc actagaaatc caagcaagcc
     6481 aagagtatga aggcatccca ttcaatgatg atacagcaaa aatcgttctt tctttagaaa
     6541 aaaacgataa tgattatagc atcacgaaat acatgcagac gcatttatct gatatcgagc
     6601 agctgcgcga aaagacggaa ctgtatacgg aagaggaagc aatcaataca ttgtacatca
     6661 ataacaaaat accaagagga tcaaaaattt tatggcgtca gctagcttat tcctgtattc
     6721 tgaaagtccg ggaaaaaaat gtgtatgttc ctgtttggca tgtagcgatc gaaacaagcg
     6781 ataaagtggt ccaaattgag agtgtcaacg ctttcagcaa tacgatcgtg acaaacaata
     6841 ctgtaccaaa agtggaagat caataaaaaa gcgagtataa tgaaaaaagt ttacaaagta
     6901 agaagggaag taggagtcaa atggctgaag aaaaaccagc ttttaaaatc agtatcctag
     6961 caagtggaag ttcgggtaat tcactttata tcgaaagtga aaaaaaacgg cttttggtcg
     7021 atgcaggact tagcggtaaa aaaatcactt ccttgatggc tcaaattgat cggacaccgg
     7081 accaattaga tggaatactt gtgacgcatg aacatcgaga tcatatacat ggcgtgggtg
     7141 tcttggctcg taagtatcat ttggatgttt ttgccaatca gcagacttgg gaagcgatgg
     7201 aaccactgat tggaaatgta ccagtggaac aaaaacatct atttgagatg gggaaagtca
     7261 gaacattcgg cgatatcgat attgagagtt tcggcgtctc ccatgacgca gcggctccgc
     7321 aattttatcg tttccataaa gaaaatcggt cattcgtcat gttgacggac accggatatt
     7381 gcagtgatta tatgcgaggt gtcattgaaa atgccgatgg ttatttgatg gaaagtaatc
     7441 atgatcttga gatgttacgc atgggacctt atccgtggaa tttaaaacag cgtattttag
     7501 gagatagagg acatctttcc aacgaagacg gtgcacttgt catgactgat gtgatcggta
     7561 atcggaccaa acgaatctat cttggccatc ttagcaagga aaacaatatg aaagaacttg
     7621 ctcatttaac aatggaaaat gttttgaaag aaaaagattt tggcgttgga catgattttg
     7681 aaatctatga tacagatcca gatgaagcaa ctgagctatt ttcgatttaa acagttcaat
     7741 caataaaaat ataaaaagca gaagataaaa tcaatcaggg ttggttttat cttctatttt
     7801 tattttttta gcatattaaa aaaataaccg tattatcgac tgactacact gatgtgctgc
     7861 ttttcatgtt tctcatccag tatctcatct actaatgcaa tcgcgtaatc tgccatgctg
     7921 atcgtactgt cgccagcagc gtttctttgt aaataatcat cacttaacaa ataagtacct
     7981 gtacgtttgc catgtggatc aaataaagaa gctggactga tataagtcca atgtagattt
     8041 ttagattgtt tcaattttaa caatccttcg tacatattga aagctgttgg atagaagggt
     8101 gcatttttag gtacttggtc aatcatacga gtcgtctttt ctgtatctaa gaataacgaa
     8161 gaggcacccc caacgacaat taagcgtgta tcggttcctt caagcatctc aatcaaatgg
     8221 gacaaagaag tttgatgcat ttcttctttt cctttgggag cgttgaaagc atcaacgaca
     8281 acatcaaaag atgcaagatc agtagttgtc aactcaaata aatccttttg tataaacgga
     8341 acatctgatg gcaatttttc tggatgacgc acgattgccg tcacgtctaa atctctggac
     8401 aatgcttcgt ctaaaataaa tgaacctgca tggccagtag ctccaatgag tgcaattttc
     8461 ataaaaaacc tcctttttta ttcctgtcca ttgtaagcag ttgctgaata ttcgtcaaag
     8521 aatccgattt ttgaaaagct cagtgcgcat tcctttcatg gttcgtgatc attgggtata
     8581 gttaaattaa gaaaactgat tgataaaaat agaagttgaa ggagataaga aatgaaagag
     8641 tggttagatc gtgccttttt accaatgata acatctgttg tgaatcttag acccttaact
     8701 gctttgcgta atggaatgac ttacacgatc ccaataatta ttgttggctc gctgtttttc
     8761 ttatttgctc attttcctgt ggattacatc gctcagtgga tagacaaaac aggattttct
     8821 atgtatttta cacaagtata ccaggctaca tttgatgtga tagccttttg gatcgttttt
     8881 gccattgcat acgcttatgc aaaagaagaa gacatcgatc gcttaccagt cggcataaca
     8941 gccttgtctg cttatctact gcttattcag ccactaaata ctcagataga tactgtctgg
     9001 acaggtgctg aagggatact aggagcagtt acaactgggc tcatcatcgg atggggctat
     9061 gcacgtttag taagacttgc gaaagggtgg aagcaaagag agaagatacc tgaaaatgtt
     9121 tggcagtctt ttacttcatt gattccaatc attgttatag tcacttttac gcttatcggt
     9181 tcagcggttt ttcaacagtt gttcagaatg actgttgttc aaggagtatg gacatatgtc
     9241 cagcttccca ttcaatcgtt catagacacg ttgtttggtg tgcttttatt aggttttttt
     9301 gttccgtttt tatggttatt tggtcttcat ggatctgcga tggtcaatgg gttggttagt
     9361 cccgtcttgc aagcgaattc attagcgaat gcagaaatct tagcctcagg acaagagttg
     9421 acggtggcaa atggcggaca tatcgttact cagcaatttc tcgatcagtt catgacggtt
     9481 acgggtgctg gattgacgtt aggggctgta ttttttatga cggtttttgc gaaatcgaag
     9541 aaatacagag aattaggaaa tcttgctctt ttaccagcct ttttcaacat caatgaatca
     9601 attatttttt ccacaccgat cgtgatgaac ccgatgatgg ctgttccttt tattttcgca
     9661 ccggtcgttt caggattgat tacctattcc gctttatatt ttggattcgt tcctttattt
     9721 acagcagtcc aagtcccttg gacaacaccg ccgatcctct cgggtttctt agtaggcgga
     9781 gtgcctgcag cgattttaca aggtatcgtg ttgtcaatta gttttttcat ttatttccca
     9841 tttttgaaaa aaatagattc tgcaaactgc aaaaaggaaa gacagacaaa aaatgatgga
     9901 gcagcagaaa agatgaacga ctaaaaaaca aaaatactcc tgtcgcttta gattgggaaa
     9961 atcaccaatc taggatgaca ggagtattta tagtcatgtt tatactagcc ctttttggac
    10021 agctaattga acaagaacaa ctaatgtgtt cattcctacg tgtgtgatca tcgatgtcca
    10081 gatccgacca gttgctttgt actctaggct gaagaagaaa cctaggaaaa agtagattag
    10141 cagatgacca tcattatgag caaacgcaaa cagcaaagag ctgatcactg ctccgaccca
    10201 aaaatttgaa tatcgactga tgatccccag tatactgcgg cggaatacga actcttccat
    10261 gatgggtcca gcaacagttg tcgccaaaat aaaagcgggt gcttccatga tcatctggat
    10321 gatattttgt gtattctcgg aaggggttgc tttgccaaac aatacagatt ctaacatgac
    10381 agcgatgctt tgcagaatca atgcgacaaa gattccaacc aatccataaa taacaatctt
    10441 tggagcagaa gcttttttta ccgctgattc gatcgctaag ggttcacgtt gcttaaaata
    10501 aaaaatagct aggacaacgg cgcctagtat atacatgact gcagttgctg tgatcaattc
    10561 agacgtagat tttacaagtc ctatcgaagc taggatcaag ggtgagataa aaattagacc
    10621 atagcaaaaa atgctgagga agctatattt ttttaaagac atgtaattcc tccttttaag
    10681 ggataacaag catgcaaggc aagcttatat cgattgattt caattatatc ataatttcaa
    10741 taagcagctg tcagaataaa atttcttttc tttgatttaa aagcttgcaa aaaagaggga
    10801 gaatgagtat tattaaaaat gtaggttagc actcgaaggt taagagtgct aacagattaa
    10861 aaaaagagtt atcggaggga tttatcgtgt taaaaccatt aggtgatcgc gtcattatcg
    10921 aagtcgcaaa agaagaagaa aaaactgtcg gaggaatcgt tttagcttct gctgctaaag
    10981 agaaacccca aactgggaca gttgttgcag tcggtgaagg ccgtttgtta gaaaacggtg
    11041 aaaaagttcc tgccgctgta aaagcaggcg accaagttat gttcgaaaaa tacgccggta
    11101 cagaagtaaa atacgaagga aaagaatatc tgatcgtcgc aggaaaagat atcatggcga
    11161 ttgttgagta aatttggaag ataaaaaaat aatcaaaaat aatcaatgag gtgaatcaat
    11221 catggcaaaa gaattgaaat ttgcagaaga cgcacgcgca gctatgttac gcggggtaga
    11281 taaattagca gatacagtga aagtaacatt aggacctaaa ggccgaaatg tcgtactaga
    11341 aaaatcttac ggctctccat tgatcacaaa tgatggtgtg acgatcgcaa aagaaatcga
    11401 attagaagat cattttgaaa acatgggtgc aaaacttgta tcagaagtag catcaaaaac
    11461 aaatgatatt gcaggagacg gtacaacgac agcaactgtt ctgacacaag caatcgttcg
    11521 cgaaggttta aaaaacgtaa ctgcaggtgc taatcctcta ggcattcgtc gcgggatcga
    11581 attggcaaca aaagcagcag tcgaagaatt gcataatatc tcaactgttg ttgattcaaa
    11641 agaagccatt gcacaagtag cagctgtttc ttcgggttct gataaagttg gacatttgat
    11701 tgctgatgca atggaaaaag ttggaaacga cggtgtcatc acaatcgaag aatcaaaagg
    11761 gatcgaaaca gaactagatg ttgtagaagg tatgcagttt gatcgtggtt acttatcaca
    11821 atatatggta acagataacg acaaaatgga agctgttcta gaaaacccat atatcttgat
    11881 cacagacaaa aaaatctcta acatccaaga tattctgcca ttgttagaac aaatcttgca
    11941 acaatcacgt ccattgttga ttattgctga cgatgtagat ggtgaagcat tgccaactct
    12001 tgtattgaac aaaatccgtg gaacattcaa cgtagtagct gtaaaagctc ctggtttcgg
    12061 cgatcgtcgt aaagcaatgc ttgaagatat cgctattttg acaggtggta cagtaattac
    12121 tgacgatcta ggtttagaat tgaaagatgt aacaatcgaa aacttaggaa atgcttctaa
    12181 agttgttgta gataaagaca atacaacgat cgtagaaggt tctggagaaa aagaagcaat
    12241 cgaagctcgt gtgcaattga tcaaaaacca aattgctgaa acaacttctg attttgaccg
    12301 cgaaaaatta caagaacgct tagctaaatt agcaggtggt gtagctgttg ttaaagttgg
    12361 tgcagctact gaaacagaat tgaaagaatt aaaattacgc attgaagatg cattgaatgc
    12421 tacacgtgcc gcagtagaag aaggaatggt ctcaggtggt ggtacagctc tagtgaacgt
    12481 tatcagcaaa gtttctgctg tagaagcaga aggagacgtg gcaacaggta tcaagatcgt
    12541 tgttcgtgct ttagaagaac caattcgtca aatcgctgaa aacgctggtt atgaaggatc
    12601 agtgattgtt gataaattga agaacgtgga actaggcact ggtttcaatg ctgctactgg
    12661 agaatgggta aacatggttg aagcaggtat cgtagaccca actaaagtaa cacgttctgc
    12721 cttgcaaaat gccgcttccg tttctgcctt gttattaacg acagaagcag tcgttgcaga
    12781 taaaccagaa ccagcagcgc cagcagctcc tgcaatggat ccatcaatga tgggcggtat
    12841 gatgtaagaa aaagcaactt gcttatctac aactgatcat tagtgaaaga cagataaaaa
    12901 gaggctggaa cagaagcgtt taactccaag aaataagaag aaattcacgg aaattgcttt
    12961 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgctgtttat
    13021 acgtgtttag agcgt
//
LOCUS       DACCYH010000080        13029 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.084, whole genome shotgun sequence.
ACCESSION   DACCYH010000080 DACCYH010000000
VERSION     DACCYH010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.084"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..1716)
                     /locus_tag="IVY85_002212"
     CDS             complement(<1..1716)
                     /locus_tag="IVY85_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8045215.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLF"
     gene            2018..2953
                     /locus_tag="IVY85_002213"
     CDS             2018..2953
                     /locus_tag="IVY85_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP8045216.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3043..4146
                     /locus_tag="IVY85_002214"
     CDS             3043..4146
                     /locus_tag="IVY85_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP8045217.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            4159..5112
                     /gene="menA"
                     /locus_tag="IVY85_002215"
     CDS             4159..5112
                     /gene="menA"
                     /locus_tag="IVY85_002215"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP8045218.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(6047..7393)
                     /gene="gor"
                     /locus_tag="IVY85_002216"
     CDS             complement(6047..7393)
                     /gene="gor"
                     /locus_tag="IVY85_002216"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP8045219.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(7527..8768)
                     /locus_tag="IVY85_002217"
     CDS             complement(7527..8768)
                     /locus_tag="IVY85_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8045220.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(8786..9709)
                     /locus_tag="IVY85_002218"
     CDS             complement(8786..9709)
                     /locus_tag="IVY85_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8045221.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(9930..12422)
                     /locus_tag="IVY85_002219"
     CDS             complement(9930..12422)
                     /locus_tag="IVY85_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP8045222.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(12429..12896)
                     /locus_tag="IVY85_002220"
     CDS             complement(12429..12896)
                     /locus_tag="IVY85_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP8045223.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         3806 a         2704 c         2292 g         4227 t
ORIGIN      
        1 gaataatcca gcaatcaatg ctaatgccaa ggcaatttca aagaatacca tgaatttttg
       61 gaagaacatt gctacatcat tgtttggcat gatcacttgc atgatctttg tgaaccagtc
      121 tggtgacttc tcaatcacag ccattggttc ttcaccgtat gcataactca aaccaaaatg
      181 tgcagcttga gtcgctgctt ctccggcact tgcccctgct ccgctggcag ctgtggttgc
      241 atcaccagca gcagaagcac cactggcaac atctgttaca ttgtattccc atgtccaagg
      301 gaacacactg tttccagtga accatgatcc ttcaccaaac caagtacttg gtttgaacac
      361 ttcaccattc ccaatgattt ttttggaagc ttcaacgaac caaactaaac catagaaaat
      421 acgaagtggc acactccata agacattgcc gtaacgagaa gaatgtcctc gagtcacgtt
      481 gcgatcatct ttgatatgga aaaactcatg cattaaatat tggaacatat aataaccaga
      541 acgaatatca aagaagtatt tcaaattcac gatatgtttc attacgatag ctaaaaatcc
      601 gcttaaatgg atcttatcaa ataggttagc taccccccat ttggcaccaa tagaaaccat
      661 aaatccttga tagtttcctt taaatttatg cttttctccg cctttgatcg aagccacgat
      721 attcgctgcg gctgtgtgtc ctgtttgttc agcagcttga acgatctgcg gtgtcggtgt
      781 tcctgggaat tcttcataaa agactaaatc accaatgata tagatgtttt tatcttcgta
      841 gccttttgcc tgcatatatt cattggcgat caaccgctgt ccgcgtgctg attctaatcc
      901 aaagtcagca gcgtcagaag ttcctttgac accagctgtc caaatcagtg tgtgagtcgg
      961 aatgttgcgt ccgtctttca atttgatatg atcagcagcc acttctacga ttggtgcatt
     1021 caataataat tccacgttgt gcttacgcaa atagcgttca gcttttgcag catcatttcg
     1081 atccaacata ttcaagatcg tcggcattgc ctctacgacc atcagtgtga tttcgtttgg
     1141 atctaattta taatctttgg ctaggcgatt tttccagtcg atcagttccc caatcatttc
     1201 aataccggta aatccagaac cacagacaac gaaagtcagc atggctttac gcagttcatc
     1261 atctggttcc aatgcagctt ttgctactgt cttctcaata tgttcccgaa tccttacaga
     1321 atcttcaaat gaccaaaggg tgaatccgtg tgctttgaca ccaggtgtac caaagtcatt
     1381 cggctctccg cccattccaa ggatcagctg atcaaaagga taggaaccta atttggtctt
     1441 gacgattttt cgttctttat cgataccgat tacagtgtct gtgacaagtt gtacattctt
     1501 ttgacgagca aacaaatgct gcagatcgta ttggattgct tctggttcta cacgtccgcc
     1561 agctacttca tgcaactcgg tcatcatagt atggtacgaa tgacgatcga tcagagtaat
     1621 tgctacatct tcttttttca gtttctttgc caagtatttt gtagctgaaa caccggcata
     1681 tcctgctcct acaacgacaa tattttgctt tgccattgat catccgctcc ttatagacta
     1741 tctatataat gaatatatga taattctttt agtttgatac ttattacaca atctccatat
     1801 attatataga tgttctgttc ttttgtctaa ctccttttgg atttttttca aattttaaaa
     1861 taaagcgttt tttcacgaaa tttgctgaaa gaaaacgttt gaaatattac atgggttatt
     1921 tatcaaaaat agttgactta taggctaaaa agattagaat gtataatgaa tcacaaagaa
     1981 aaattgtgga ctagaaatgg aatggtgggg tattaaaatg aagaaaaaac gttttgtaac
     2041 tggatttgcc gtattagcat tttcagctct agtgttaggc gcttgcggcg cagatgacaa
     2101 cggaagctca aactcttctt ctgaatcatc tacagcacaa tcttcgactg caaaaagcac
     2161 aacagaatca tctgcaaaag ttgtagctgg cggagatttg caagacggca catataaatt
     2221 agaagaaaaa aattattcaa atggttaccg tgcagtcttt gaaatggttg taaaagacgg
     2281 caaaatcact gaatcaaaat atgataacgt caatgaaaac ggcgaatcaa aaacaaaaga
     2341 tgcagaatac aacaaaaaca tggaagcaaa atctggcaca aacccagaaa aatacattcc
     2401 tgagttgaat gaccaattct taaaagcaca aagcgcaagc ggtgtggaag tcgtaactgg
     2461 agctactcac tcatctgaaa gcttccaaaa ctacgcacaa caattgatcc aagctgctca
     2521 agctggtaac acagatacaa tcgaaatcga caatggtgct gatttgaaag acggcactta
     2581 caagcttgaa gaaaagaact actctaacgg ctaccgtgtg caattcgaaa tgacagttgc
     2641 tggcggcaaa gtaactgaat caaactttga ttatatcgac aaagacggta aatcaaaaca
     2701 agatgataca gaatataatg aaaatatgaa agcaaaaagc ggtacagaac caaaaactta
     2761 catccctact ctgaacgacg aattcgtcaa agcaatgggc gaagaagatg gctcacctgc
     2821 taatgtagaa gttgttacag gtgctacaca cagctcacat tcattcatca tgtacgcaca
     2881 acaattagtg aatgcagctg aaaaaggcga cactcaaaca atcgaagtag ataatatcgt
     2941 aacagaaaaa taatcacata atcatactaa taaaagttgg agggtgggac gatagccaga
     3001 ttaggcagtc gtctcacctt tcaaaatgaa gagaggaaaa agatgcggaa aaaactacta
     3061 ttttccttaa tcacactggg catctctttt atggtgctaa ctggctgcag caactcagca
     3121 aataaaacaa ccactgaaag ttcagcagct accaagatat tgaaagatcc atattctgat
     3181 gaacagttct tgttagggac ttacgttcgt atccgtgtgt atgatgaagg caaagaatca
     3241 gcattgaaac ctgccttcga tcgagtaaaa gaacttggtg ataaaatcac gatcaatcaa
     3301 aaaggttctg agatcgatga agtgaatgag caagctggga tcaaaccagt aaaagtttct
     3361 gatgacgtct acacgttagt caagcgggcc tacgaatata gccaagattc tcaaggcgga
     3421 ttcgatatgg cgattggtgc aatcacacaa ttatggcgaa tcggttttga tgacgccaga
     3481 aaaccttccc aagaagaaat cgatcaggca ttgaaattag tggattatca taagatcgaa
     3541 ctaaatgaca aagaaaaaac tgtttatcta aaagaaaaag gaatgatcat cgatttaggt
     3601 gcaatcgcca aaggttacat tacggacgaa gtagtgaaag tcttgaaaaa acaaggagtg
     3661 accactgcca tcgttgactt gggcggtaat gtctatgtct taggccacag cccaagaggc
     3721 gaaaaccaag actggacagt cggcatccaa gatccgaaca tggctcgcgg atctgtttta
     3781 ggttctatta aagaacggaa caaaacatta gttacctctg gaatttacga gcgttattta
     3841 gaagtagatg gcaaaaaata ccatcatttg tttgatccaa aaacgggtta tccgttcgac
     3901 aatgatatcg caagtgtcac gatcataaca gataaatcaa ttgatggtga cgggttgtct
     3961 acagcagtct tttcaatggg tgtgaaaaaa ggactggaat acgtcgaaag cgaattgaat
     4021 aacgggacgg aagcgatctt cgtgacaaaa gacgataaag tatatgtgac cgatccaatc
     4081 aaagacacct tcaaattatc ggaggactcc cattacacga tgggtgaccg ttctgaacta
     4141 aaataaggag tacgaaccat gacactaaaa atctttttag aagtagttga aatacgaaca
     4201 aaggtggcta gtgtatttcc ttttgcggta ggggtattat tctctatcgc tttttttcat
     4261 gagatacatt ggctaaacac ttttttattt tttattggaa tgctagtttt tgatttagcg
     4321 actaccgcta tcaataatta catggattat gaaaaggctc attctgaagt atataagtat
     4381 gaagaaaatg tcattggccg cagaaatcta tctcctgtat tgattcgaaa catgatttta
     4441 gctatgattg catttgtttt tgttatcggc gttattctaa cgatcaatac gggttggctt
     4501 ttacttttga tgggatttat ctgttgtttt attggagtat tctatacatt cggaccgatt
     4561 cctttatctc gaatgccttt aggggaaatt ttcagtgggg tgactatggg ccttggaatt
     4621 tttgccatgg tcgtttatgt caatacattc ggttcagatg tctatgatct gacagtcaac
     4681 ttttcatctg gacaatttgc tttggtagga aatctttggg ctatagcagc aatcgtatta
     4741 gcttccttac ccttggtgtt tacgattgcc aatatcatgt tggctaataa tttgcgggat
     4801 cttgaccgtg atattgaaaa tcatcgctat acactagttt tttatatcgg acgtcctgcg
     4861 ggaacagtct tattccagct attgatgtat gcttgctatt tcgtgatttt aatggggttg
     4921 ttgagcggta tctatgaatg gccgatcttg gtcaccttct tgaccttgcc aattgtgaag
     4981 aagcatttgg atcaattcaa gcaaacgtta ccccagccta tcagtttcag ctattccatc
     5041 aaaaatatgg tcttattcaa tgccagttat gtattggggc tattactaac aatcattatg
     5101 caagccattt aacagcatga agaggttgtg aaaaaactgc cctgcatcaa gtaataagaa
     5161 cagccaaata aaaatagctc cttatatttt tcgtttggtt gggcttatta ccgcagatgc
     5221 aagcttttga acaccgttta tcaggaggtt gtgaaaaagt cgcttagttt cgagtattaa
     5281 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgagaa
     5341 actggctttt gaacaccgtt tatcagaggt tgtgaaaaag tcgcttagtt tcgagtatta
     5401 agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt aataccgaga
     5461 aactggcttt tgaacaccgt ttatcagagg ttgtaaaatc agtgttttgt cctgagtatt
     5521 agagaaacaa atggaaaaat agttcctcat attttgccgt tttttgagct aataccgaag
     5581 gacagctgat tgaacactat ttgttcggaa aaagtgacta actatgacac aatttttttc
     5641 acagtccctt ttttcacttg atttgattcg taccagcgaa cttgacgaga actttcttgg
     5701 tttggtaact atatggtatt cttttaacat cctcgtttgc ttcttctaat ttatttatac
     5761 aaagtcacac ttttttaaac atctgttctt cgatacattt ctatttcttt taaacatcga
     5821 taccaatatt ttgaacttaa gagtgcatac caaatggatt catttactct tcgtcagtta
     5881 cgaagataat caaaatctcc aacttcccta cttatttcgt tttgagaatt atcgaagcaa
     5941 tcttacataa ataactaaaa aacaaaaaac cagaaaatca gtatactgat tttctggttt
     6001 tacatggttt gttcaataaa aagctgttgg aactgttaat attaaattat cgcatcgtta
     6061 cgaattcttc cgctcctgtt ggatggatgg ctactgtatt gtcaaagtct tctttagttg
     6121 cacccatctt gatagccacg gcaaaacctt gcagcatctc atcgacacca acaccaatag
     6181 catgtaagcc gacgattctt tcttcttttc cgacacagat caatttcatt tcgcattttt
     6241 ggcgataatc atttaatgca aagtacattg gggtaaagcg tgaacgataa actttcaatt
     6301 cattttcgcc atatttttcc aatgcttctt cttctgtcaa accaattgtt gcaattggtg
     6361 gatgcgtaaa gacgactgtc ggaacaagtt tatagtcaag ataagaatct ttctttccat
     6421 taaataaacg ttctgataaa cgtctaccag ctgcgattgc aacaggagtc agatctaatt
     6481 taccgatcac atcaccaaca gcaaaaatat gttcatccgt tgtattttgg tatttatcta
     6541 ctttgataaa cccttttttg tccaattcca catttgtatt ttccagacct aatttttcgg
     6601 tattcggtac acgtccaccg gcaaaaagta cacaatcacc agttaaggtt tcaccatttt
     6661 caaatagcac cgtatattcg tcattgtttt tagtaatttc tttagcaata tgatgtgcgt
     6721 aagtttttat tccagcttct tggtacattt ggatcaaatt gtccgaaagc attttatcaa
     6781 acgttctaag tggtcgttct tttcgatatg cccacatgac ttcactacct aaaccacgaa
     6841 atacacctga aagttcagcc gcaatgtatc cagcacctaa aacgattgct ctcttaggta
     6901 actctgttaa ttcaaaaaat ccatttgaat caattgcgta ttctccacca ggaatggtca
     6961 ttttacttgc acggcctcca gtagcgatca agattttagg agcacgatag gtttcaccgt
     7021 ttacttcgat cgtttgacta tcgataaaag tagcgtaacc ttttatagcc tcgacatgat
     7081 tgctatctaa tcctctttga taagcaccgt gtagaaaatc aatatatttt tctcgtcttt
     7141 ctactagacc ggcgaaatca acatttttta aatctgcttg gataccgtaa cttgaggcat
     7201 ctcgttcaat tgtttccaaa atcgtacttg cttgccacat aactttttta ggtacacaac
     7261 cgacgttgac acatgttccg cccaattcat tcgcttcaat cagcaaaact tttgcgccat
     7321 gcatccctgc tctgtttgct gaagcaatac cgccagagcc gccaccaatt acgatgtaat
     7381 catatatttg cattttgttc ctccttattg aagagatcaa tcaaaacctt tatttaacga
     7441 tcctttttct acagaaaaca agtgtaacac ttttcttgaa ttttgtatgc gaagatgctt
     7501 aaaacaggga tatatcagct ttttccttat ttacgttttc ggatcatatc tccaggtttc
     7561 accggctgtg cgactggcat agttaaaatc atcattggat ttggtgctcg gtcaatggat
     7621 tcgccttctt cattatgcat aacttcaacg gtttgactga aatgactgaa tcctggacca
     7681 taaaattcga tttcatctcc tacagaaaac aagtttctct gacgtatcgt agcaatctgt
     7741 gttgcctcat cataagataa tacttcaccg acaaatttat atactgggat tttacgacgt
     7801 tcaccgaata attgttcgtt ttcagttggt gtattgtagt aaaaacctgt agctaattct
     7861 cgttgtgcta ctttccataa ctcgtcgatc cattcttgct tgcagacata gttttcagga
     7921 tcttccatat aactgtctac agccgcttta tagacatttg acaccgtaga tacataatgg
     7981 atcgatttca ttcttccttc gatcttgaag ctgtctacgc cgttttcaat caattctgga
     8041 atatgttcga tcattgacat atccactgca ctcatcgaga attcttcttc cacttcgcct
     8101 gtatcagtta aactgtttcg ttcttgacca aaaggcatat cgaacaatcc atacttccag
     8161 cgacaagatt gagaacatcc gccacggttt gcatcccgca tcgacatatg gttggacaat
     8221 gtgcatcgac cagaataaga gatacacatg gcaccatgga taaatgcttc gatttcggta
     8281 tccgtatttt tacgaatctc tgccacttcc tccattgaaa cttcacgagc taaaacgaca
     8341 cgttccaacc cttcttcttt ccagaattcc aatgtctcaa aattagttgc tgaagcttga
     8401 gtggataaat ggatcggcag acctggggct tctgtcgcac aaatttcgat caaagctgga
     8461 tcagaaacaa tcactgcaga aataccgaca tcacgcaaac tgcggaaaaa ttcccctgct
     8521 ccatcttcat ttccttcatg ggtgaccata ttagcagcaa cgtatacctt cgcatcatgt
     8581 tcttttgcaa aagcaactcc ttcagccatt tcttcataag taaaattacc tgcacggctt
     8641 cttaaaccat atgcattccc gccaatatat actgcatctg ctccataatg gatcgcggtt
     8701 tttaactttt ccagtgttcc tgctggcgct aggatttctg gtcgtttcaa ttttcttttt
     8761 actgtcattt ctttcatcat actccttatt tgatagcaga aggatcaata taaaagaatc
     8821 ctgtatctaa ttcacggttt tcaggatgca atttactgat ttcgctagtc attttttccg
     8881 ctttttcttg atcccagcta ccttctaaaa tttgtttttt tgcttcggcg aatagtcgag
     8941 tgatgtttac gaatttttcg cctggagtaa ataatccgtc caatttccat gtacagtaat
     9001 gatgctgata aagttcattc aattcattcg agagattcag atcattgctg gcaaaaatat
     9061 gtgtgccatg actatcttca taaatagaat aatgtgtctc ttctttttta ggttccgaga
     9121 taaaaagacc tctttcacga tctttttgtt cgtcttgttt cgtatagtta tagtaattct
     9181 gtagcagtgg acgtttggat tgatggatgc atgtagcacc atacacaagg gtttctacag
     9241 gaatatcgag tttttcttcc atcgccttca tctcttcaaa aggaacttca cgagcaagta
     9301 ctgctccaac tgcgcctttt ttcgcccaga aattgatttg acgtgcgctc gttactaatg
     9361 tttcagcatc ataaatatac ggtatacgca attcaggatt ttttttcatc gtataaataa
     9421 tacctgtatc accaactgtg atcacatcta cgtttatttc ttttaaaaaa gccaaatatt
     9481 caggtacatt tttcattttt tcaggatgaa tgatcccatt tacagcaata ttcgcttttt
     9541 taccatactt gtgtgctaat tctaccaatt gtcgctgttc ttctcgtgta aatgaagcag
     9601 gcagacgcaa tccaaattgc tcttcaccga aatatagtgt atctgcacct gcagcaagca
     9661 actgttctgc ttgttcaata gtttctactg tggttatgag ttcgatcata attttctcct
     9721 cctacaactt cataatagta gcatagctct aaaaactttc caatacttct tctatttaaa
     9781 gtcggatttt tgaaaattat ttcacaaaaa atcaaaataa agttcgatat attctgtcca
     9841 aatataaaaa gagattgtga caaaaatctt gtcacaacct cttaatcaag tcttactatt
     9901 ataaaaattc agctacacag gccaagtaac tatagggaac ctactgtttc ctttttaggt
     9961 ccgcgaacat tcaatgtgat cttacctttt gaagcaccta ctgttacgtg gtcacctaaa
    10021 cgaatttctc cgccaagcaa agcctcgctc aatcgatctt caatttcttt ttgaagtgcg
    10081 cgacggattg gacgtgcacc atattcagga tcaaatccag ctttcccgat cacatcgatt
    10141 gcagatggtg tgattttcac ttgaatatct tgttctttca atcgtttgat aatcgctttg
    10201 ctcatgattt ggacgattgt atggatttca tctttactca atgaatggaa gacgacagtc
    10261 tcatcgattc gattcaagaa ttcaggtcgg aaagctttct ttaactcttc taagatacgt
    10321 ttttgcatgg ctttgtgatc tttagtaaca tcttggacat tgaatccgac atttttttct
    10381 tctcgaattt gtgtcgctcc gatatttgaa gtcataatca tgattgtatt tctaaagtcc
    10441 acttttctac ctttagaatc tgttaaatga ccatcatcca atacttgcag aagtaaattg
    10501 aaaacgtctg gatgtgcttt ctccacttca tctaagagaa tcactgaata tggtttttga
    10561 cgaatttttt ctgtcaactg tccaccttca tcatacccta cgtatcctgg aggagacccg
    10621 attaatcggc ttgtactgta tttttccata aattctgaca tatcaacacg gatcagggca
    10681 tcttcacttc caaacatgac ttctgaaagt gctttggcca attctgtttt tccgacacca
    10741 gttggtccaa ggaacatgaa tgaaccaatt ggacgatcag gatctttcaa tccgcttcgt
    10801 gcacggcgga tcgcacgtga aactgctttg acagcctcat cttgaccaac gacacgttca
    10861 tgcagaagtc cttccagttc taacaagcgt tcgctttctt ttttctccaa ctgctgtaga
    10921 gggacaccag tccactccga tacaactgta gcaacatctt cttctgttac acgatcagaa
    10981 taaccttgct cctgttttgc ttcaattgca gaaacttttt gcagcttttt acgtagtgct
    11041 ttttcttgtc tgcgtaattg agcagcattt tcaaagtctt gtttttggat cgctgcttcc
    11101 ttttcttgta ctagttcatc gatttctagt ttgatcacag ttgattttgt taagtgatcg
    11161 gtttgatcca aacggacttt cgctgctgat tcatcgatca aatcaattgc tttatcgggt
    11221 agttgacgag aagtgatata acgaacagaa agattgactg ctgctcgtac agcttcatct
    11281 gtgatctcca ctccatggtg ctcttcatat cttgtacgta atccttgaag aatcacttct
    11341 gcttcttcag gagttggctc atctacttga atgcgagcaa aacgtcgctc taatgcagaa
    11401 tctttttcga tatatttttg atattcatct agggtcgttg ctccgatcgt ttgtaattct
    11461 cctcgagcta acgccggttt caagatattc gaagcatcaa ttgctccttc tgctccacct
    11521 gcaccaatta aggtatgcaa ttcatcaatg aataagatta cttgaccatc attatagatt
    11581 tcgtcgatga cctttttcat tcgatcttca aattcaccgc gatatttcgt accagcaacc
    11641 aaagatccca tatcaagcat catcaggcgc ttttcttgca tgtcttctgg cacttcacct
    11701 aaaatgattt tttgagctaa cccttcagca atggctgttt tgcctacacc aggttcaccc
    11761 accagtacag ggttattctt tgtccgtcgg ctaagaatct ggataagccg tttcacttct
    11821 ctcgaacgac caacaactgg gtccagcttg ttttcttttg ctaatttcgt caaatcacgt
    11881 gctaatgaat ctagcgtagg tgttccttgc ggtgtttgcc gtttatcttg tacatttaca
    11941 tttctacgac gattcattcc ttttccagca gaatcaccag ctagccccat tttttgtttc
    12001 agaacttgac gggcttttga aaggctgata cctaagttga tcatgattcg agtagccatg
    12061 atactttcat cttttaataa gctcatcaaa atatgttctg ttccaacttt ttgagaacca
    12121 gttttcttca cttcgctgct agctaaagca aaaacttgtt tcattcttgg tgaatatgga
    12181 agaaagactc ctttagggta agaacggata ccaccataac caatcagatg ttcaatttct
    12241 tcataaattt ccgtttcatt caaatccatt tcacgtaata cttttccagc aattccattt
    12301 tgctcgacca ctaaggctag taaaacatgt tctgaactaa ctgattgatg tttcaaccgt
    12361 tttgcttctt cttgtgcaat ttctaatact gcttgggctc tttcagtaaa aagttcttcc
    12421 atctaatctc acttcccatc ttcataactt aaacgttcca gaaatgctcg gagaatccga
    12481 gcacgtgtat gatcttcata ttcattgaaa ttcaatgtgt tttttgcaat tgcactcaac
    12541 atcaagtttc cttctttttt tgtaattatt tggtcttcat aaagtttttg aataatagca
    12601 aaagcatttt tctcactgat tgtatcatca aataactgat ttatctgctc aagtaattgt
    12661 tttttatctg aaatccttac ttttgcaatt cgtatataac caccaccacc acgtttgctt
    12721 tctactagat aaccacgttg gatggtaaaa cgggtattaa tcacatagtt gatttgtgaa
    12781 ggaacacagt taaagaggtc tgccatttca atccttcgaa tctcgatcat ttcactattt
    12841 tccagaattt tctttatata tgattcaatt aaatccgagg tattctgatg tgccatctta
    12901 ttcattccct ttgactaata ttgaccttaa ttataacgaa tttgataata gaaagaaaga
    12961 agaacgcttt ttgttaaata aatcctttag aatcaacaaa aaaaaatacg gaatatacta
    13021 ttccgtatc
//
LOCUS       DACCYH010000081        12995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.085, whole genome shotgun sequence.
ACCESSION   DACCYH010000081 DACCYH010000000
VERSION     DACCYH010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.085"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            87..1067
                     /locus_tag="IVY85_002221"
     CDS             87..1067
                     /locus_tag="IVY85_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8045224.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            1189..2607
                     /locus_tag="IVY85_002222"
     CDS             1189..2607
                     /locus_tag="IVY85_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP8045225.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            2678..4147
                     /locus_tag="IVY85_002223"
     CDS             2678..4147
                     /locus_tag="IVY85_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8045226.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            4157..4624
                     /locus_tag="IVY85_002224"
     CDS             4157..4624
                     /locus_tag="IVY85_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8045227.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            4660..5532
                     /locus_tag="IVY85_002225"
     CDS             4660..5532
                     /locus_tag="IVY85_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8045228.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFIKSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            5613..8147
                     /locus_tag="IVY85_002226"
     CDS             5613..8147
                     /locus_tag="IVY85_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8045229.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            8303..9076
                     /locus_tag="IVY85_002227"
     CDS             8303..9076
                     /locus_tag="IVY85_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8045230.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            9069..9860
                     /locus_tag="IVY85_002228"
     CDS             9069..9860
                     /locus_tag="IVY85_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8045231.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            9870..10361
                     /locus_tag="IVY85_002229"
     CDS             9870..10361
                     /locus_tag="IVY85_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8045232.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            10379..10789
                     /locus_tag="IVY85_002230"
     CDS             10379..10789
                     /locus_tag="IVY85_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8045233.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            10793..12193
                     /locus_tag="IVY85_002231"
     CDS             10793..12193
                     /locus_tag="IVY85_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP8045234.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            12216..12590
                     /locus_tag="IVY85_002232"
     CDS             12216..12590
                     /locus_tag="IVY85_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8045235.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         4502 a         1747 c         2624 g         4122 t
ORIGIN      
        1 gaaaagggga atgagagagt caaggttctt ggaatgtcga atagtcgagt aggtagattt
       61 tctatctaca accttgttgt ttaaaaatga aaaaaattgg aattagagaa gttgcgaaag
      121 aggcaaatgt ttctcctacg actgtgtcga gggtattgaa tgatcgtgga tatttgagtg
      181 aggaaacaaa aagaaaagtt tttgatgcca tgaaaaaatt agaatactat cctaatgaac
      241 tggcgagagc actttttaaa aacgaaacat ataccgtagg tttgattttt cctacaatta
      301 ctaatccatt tcacgctgag ttaattcaag atatcgagtt cgagctttcc agcaggggat
      361 ataaagtgtt gctgtgcaat agcttaaaca atccaacaaa agaaaaagag tacttgacga
      421 tgttgagaaa gaatcaggtt gatggaatta tagtaggaac gcacaataaa gatattgcgg
      481 attatgatat tccagggcta cctattgtag cgattgatag aagtcttggt gaaaatacag
      541 ttacagtatc ttgtgataat tacaggggtg gggaattagc tactcaacta cttgtaaatc
      601 atggctgtca gaatatactg tgtattcggg gagatagtaa gataaagcta cccgcgaata
      661 gtaggacttt agcatataaa gatgttttga ataaattcat gctggaaccg ttagtcctag
      721 aagtaccatt tgtaaaagaa atcaatgaaa aaaatgaaat aattaagaca tatctggaaa
      781 ctcatccaaa tattgacggg gtttttgcag gagatgattt attggcttct ttggcgatta
      841 acaacttagg aagttcgggc aaaagagttc caaaagaggt taaagtgatt ggatttgatg
      901 gagctcaaca aacattgatt tataatccag aattatcaac tattcaacag cctatagatc
      961 aaatatctaa agtagcagta aaaaaattat tgaatatgat taagggcgaa aaggagacgg
     1021 atgaactttc acttccagta aagctcataa aaaaaaatac agcataagtt aaatcggcga
     1081 aaaattcctt cgtcgattta aaaatatttt tatgaaacgg gttgacatat gaaacgggtt
     1141 gacatatatt agttgtgaag ataaaaacct tttcactgga ggaagaaaat ggagaaatta
     1201 ttaattaaaa gcaataatca tcaactttac taccaaccta aggatgtttg ggtcggagat
     1261 gttatgcctt acgggaaaga aggaaaattc tttatttatc atcaacgtga tactagaaat
     1321 ccagttcctt ttggagaacc gtttggctgg gctttagcaa ctacgaaaga ttttgttcat
     1381 tttgaagact ttggggaatc tcttttaaga ggggatgaca atgagagaga ccagtttatc
     1441 tacgctggtt ctgtatttga ggcaaatgga aaggttcacg cattttatac agggtataat
     1501 cgtgaattct tagcgcaggg aaagacatct caagtgttgc ttcatgctga gagtgatgat
     1561 tttgttcatt gggaaaaatc agaaacagca ttggagttag aaccacaaga agggtatgat
     1621 attcgaaact ggagagatcc atttgtcgtt tgggatgatg aaaaagaaga atatttgcta
     1681 attttagggg cgcgaaaggg tcaggataaa catctacaaa ccggacgttt agtggaattt
     1741 acatcaaaag atttaactaa ttgggaattc aaaggtgatt tttgggctcc gggattgtat
     1801 accatgtttg aaatgccaga acttttcaag attggtgact ggtggtatct tgtctattca
     1861 gaatactcag atggaaataa aatactctat cgaatgagtc aaagtttgga aggtccttgg
     1921 attaagccta aagatgaagc ttttgatgga cgggcttatt atgcaggtag aacagcttat
     1981 gatggaaaac gtcgagtcct atttggatgg gtaccaacaa aagatcaaga aaatgataaa
     2041 aacaattatt tatggggagg tactttggtt cctcatgaag tctatcaaaa agaggatttt
     2101 tcacttgggg taaaacccat agacgaagtg tggaatgcat tcataaattt acatgatatt
     2161 gccgatgtag agatagaaac agcagacaca agagaagaag taacgcttgt caaaaatagt
     2221 ggaactttgt ttaatttaga aacaacaatt tcatttgatg agaatcttag tgacttttca
     2281 ctgagattat ataaaaacct tgagacagat gaatcttacg aatatcgctt caatttagat
     2341 gaggggcgtc ttacatttga taaaaaccct tgttaccctt ggtttcaata tatggataaa
     2401 ggacttatta gacctatatc tttaaagcct aaccaatctt ataaactaaa agtgattgtt
     2461 gatgacaata taatgacact ttatattgat ggaatcgctc ttaacgctcg tgtacatggt
     2521 tgttttggtc aagcaattac tattacagca acaaatggat caatgactgc aataaatacc
     2581 aagttttcaa atgaaataaa gaaataataa acgtcaatca tgtggcaggt ctgaaaatca
     2641 aacatgacct gtcacattta aaagggagga taaatgaatg gactacaaaa aaattgccga
     2701 agatgtattg gaaaacgttg gaggaaaaga gaatatctca agcgctacac actgcattac
     2761 tcgtttacgt ttaattctta aagacaaaag caaagcgaat atggaaatgc ttgaggaaat
     2821 agaaggtgca aaaggtgttc ttttcaatag tggacagtta caagtaattt tcggaacagg
     2881 tataattgaa aaggtttaca aagagttcgt tgaattgaca ggagctaagg aagtatccgt
     2941 ttctgaaatg aaagacgaag gagtcaataa ttcaggaaat agacttcaac gagcttttaa
     3001 agttttctcg gatatcttta ttccgatcat tccagctttt attggggcag caatggtttt
     3061 aggaattcgt tcgttgatta ccactcctgg aatgtttgga atggatggct cattcgctga
     3121 tcacaatgta ttcctaaatg atttatccaa atttctgggt attattgcaa ccacatttga
     3181 ttatttacca gttttagtta tgtattctgc aacaaaacgt tttggcggaa accctatttt
     3241 aggattactt gtaggatttg ttatgattca tccaggacta gccaatcgga atgatttcgt
     3301 catgggaact ataaagcctg attattggca tttatttgga ttaagcattc cagcagttgc
     3361 gtttcaaggg ggtgtttttc cagcaatttt gacttcttgg tttttagcaa aagttgagaa
     3421 aattacaaat aaatattctc ctcaagtact tgcttacatt ttagtcccaa ctgtgacaat
     3481 tgttcttgcc aactttgcac tatttttagt gtttggtcca ttggggaatt ttatcggtaa
     3541 tgttttaggt ggaatcattg atttcctata tatgaagatg ggtgcctttg gtgcttttgt
     3601 ttttgcaggc gcgttacaac cattagttgt aacaggaaca caacatgcta ttcaaggtat
     3661 tgaggcaaac ttagtcgcaa caactggatt caattatatc caacctattt ggtcagtttc
     3721 aattattgcc caaggtggcg gttgtattgg tatgtattta ctctttaaga aaaaatctaa
     3781 agatagagat attgcgattt caagctttat tccaacactg gtaggtatta ctgaaccggc
     3841 tatctttgct gttaatttga aatattcaat cgttcctttt gtttgctcgt ttattggtgc
     3901 tggttttggt ggagcattta tgaaaatttt tgatgtaaaa gccattggac aaggattgac
     3961 tgtattgcca ggtctgacta ttgctaatcc agttcttcct tatatatttg gtaatctcat
     4021 agcattcatt ttaccaatca tttttattct cgcttatgat aaatataaag tgtttatccc
     4081 tgaagctaaa caccgtaaat ttgcagaaaa aactgactca aaaaaattgc cagttcaagt
     4141 aaagtgagat gagagcatgt taaatttttt tagaaaaaat gaaataagcg ttgtagcacc
     4201 tgcaaccggt gtttttgaaa aactggagaa attaagtgat ccagtctttt caaaaggaat
     4261 gatgggacaa ggatttgcaa ttgatgcaaa aaacgaaacg attgtatctc caatcgatgg
     4321 ttttgtttca agtatttttc ctacaaaaca tgccttagga ataaaaacaa aatcaggaat
     4381 tgagatactt gttcatgtag gaatagatac agttgagcta aatggtgagg gatttgatat
     4441 taaagttcaa gaaggacaaa aagttaaggc tggggatatt cttatgacca ttaattttgt
     4501 agtttttgaa aagaatcaca agactaaaga tgtcattgtt gtttttccag actaccaaga
     4561 cgaaattttc attccgaaac ttgaagaagt aaaacaagga caagaattgt ttgttcttgg
     4621 ctaatgataa gtaattaata atagaatcga ggaaaatgta tggtttttgg ggctattgaa
     4681 gctggtggaa caaaatttgt ttgtgccatt ggagataaag atttgaacat atttgaaaaa
     4741 gtcagctttc ccacgactac acccaaagaa acaatgaatt tagttttaga tttttttaat
     4801 catcataaag atgatttaga aggtatcgga gtgggatcgt tcggtcctat tgatattcat
     4861 tgtgaatcta aaacgtatgg cttcattaaa tcaacgccaa aacttgcttg gcaggatttt
     4921 gattttattg gaagtttaaa aaagcaactt aatattccaa ctgcttggac gactgatgta
     4981 aatgcctctt gctatggtga atatgtttct ggaaatggca gaggcctttc aagtgttgtt
     5041 tactataccg taggaacggg cataggaggt ggcgctatcc aaaacggaaa cttcattgaa
     5101 ggttttagtc atccagaaat gggacatatg cttgtaaaac gacacaataa agacaacttt
     5161 tctgggacat gtccattcca cggagactgt ttagagggct tagctgctgg tccggcaatc
     5221 gaaagaagat cagggaaaaa gggacaagaa gtgactgaaa atgacgttac ttgggaaata
     5281 gaagcttatt acattgctca gtgtgcatat aatacaacat tgatgttatc acctgatgtc
     5341 attatatttg gtggcggagt aatgaaacaa aaacacttaa aaaagaaagt acaagataag
     5401 tttattgagt tgatcaacgg atatgtcgct attcccgatg ttgacaatta tattatgaca
     5461 ccaaaattgt tagataattc tggtattgta ggttgtttag ctttagcaag agacgttaaa
     5521 gaaaaatctt aatttggaaa aaagatcgtc tcgactattt gtttggttga tgcgatcttt
     5581 ttttcaatta gtaattgaag ggacgcctaa aagtgaaaaa aaaaatgaaa gatgaagtat
     5641 tagagtatat caaaaataat ttaaagtatt acgatttttc tgctcaagat attgcaatga
     5701 aattttgtat taaaagaaat gtcgcttccc actatttgaa tcagttattt tcagatggaa
     5761 aattactaaa gaatgattct gttagaccag taatgttcaa gtataatcaa caaaagccaa
     5821 aagactgctt ttctaagttt attggagcag acatatcttt gaaatccact atagataaat
     5881 gcaaagctac tgtaatgtat ccccctaatg gcctcccatt aataataaaa ggaaacagtg
     5941 gcgttggaaa aagtttttta gcaagtttga tctatcagta tgctcttgat agaaaagtaa
     6001 ttcataatga cgcaaaattt gttgttgtga attgtgcaga ttatgcaaat aatccagagc
     6061 tattgtcagc agttttattt ggatataaaa aaggagcctt tactggggct gaaaaagata
     6121 ctgcagggat gctatcttct gctaatggag gatacctttt tttagatgag gttcacaatc
     6181 taagtgcaga aaaccaagag aaattgtttc ttcttatgga ttctcaaaag tatcgtaaac
     6241 ttggtgaaag tgtgcaatgg gagtatgcaa atgttcgcct tatcttagcc acaacagaag
     6301 ataaaaatac ctctttgtta gcaactttta gaagacgcat cccagctgaa atcactctgc
     6361 cagattacaa taatagaagt acatctgaaa aaattcaatt attatttgaa tttcttaaag
     6421 atgaagcaat tacgattgat tcaaaaatat tttgttcgat tgaacttttt actgatcttt
     6481 tatcgaaaac atttgaagga aatgttggag aattgaggaa tgaaatcaag cttctttgtg
     6541 cagaaggtta tttaggaaat aagagaaagt cttcaattta tttaggggac aaactagaca
     6601 gcggtttttg gatagatcca gaaattagta tagatttaaa aaaaatgttt cttaaatcac
     6661 tttctgaaat tgatttgatt gaactttttc aaaatcacat ttcaattgaa gaaagcatga
     6721 gtcaattaag agatagaata cttaaagagt gtgcaggttc ttctgagtat aattatttag
     6781 aaagtgacga atttatagca ttaagaaact atgttgttaa taaaatatca ccaattattg
     6841 attcgacagg tctatgttta ttagatgaat tcttggcgga tatcagtcta tttataatgt
     6901 ttattgatgt aattgaagaa acatcaagaa attttttgct gtctaagttc agaaaatttc
     6961 gtatgaaaaa tgataaacaa aaattattgg caaatgagat ttggagtagt ttagaagtcg
     7021 aagaaagtaa acaagaactt ttgttgaaat ggatactttt tcttgtaaag aagttctatg
     7081 tgaaaatacc agaaacccat tgtctgatag ttatgcacgg taaaataaca gcttctgcga
     7141 tcgcaaggga aacaaataag ttgctgaata catatgttta tgaggctttc gatatgccaa
     7201 ttgaaggtga gactagtgac ttaattaacc taatcaacaa tttttgtaaa agtatagata
     7261 cgacgaatgg actcattctg ctagttgata tgggatcatt ggaacaaatg tatgaaaaaa
     7321 ttgaatccaa tgtaataggt gatttggtaa ttttaaacaa tgtttctaca gctttagcga
     7381 ttgaatgtgg gattcaagtt tgccaaaaaa agccaattag tcatttttac cagatggatt
     7441 ttgattcgtt tcaagttaaa gttcagtatt ataaaggatt atcacaaaag aaaaatgtaa
     7501 tagtatcttg tctctctggt gaggggattt ctgaaaaagt taaggatatt ttaaaacggt
     7561 atctcgataa tcaagtggag attttgattt ttgattttaa cgcattaaag aaaatagcaa
     7621 aggaaaaaga tactatgatt tttaagaata caatttgtgt tctttcaacc actgaatttt
     7681 acattcaagg aatcgattgt ttaaatttag aaaatctaat taatgggaat caaaccttag
     7741 aaaaattaaa taagtatatg gactcggata gctgtgagtt ctgcttaaat gaattagtta
     7801 aactctttac tattgaaggc gctagtacta agcttcgctt tttagattcc aaaaaagtat
     7861 tcaatgagat agaaaaagta ctatatttgt atgaaaaata ttacgatgtc aaaatcccaa
     7921 gttttctgag aattaatcta tttctacatc tttctggaat gattgaacga attatgattg
     7981 gtgatggaat aacaaatgaa catttgaaag agggagcgca actatttgat acttttttaa
     8041 atgtatcaaa agattttttt tcagaaatca aagatattta ctgtattgaa atacccaaag
     8101 gagagtatga gttaatctat acaatttttg agcagactat tttctaaata gcatactagc
     8161 gtctaaaaaa atgaaagggt gtccaaagta tatttagacg ctcatttaat taatatttac
     8221 tgtattttcc cgctttattg gataatgtta gttggcacag taattgctta tataaaagta
     8281 taagaagaaa ggagagaaca aaatggcgtt ttggcaaaca tttttagttg tgttgtatgg
     8341 atttttcatt aactgggata aggaagggcc caaaatcgga acctcccaac ctgtcgttgc
     8401 aggtttcttg accggactta ttatgggaga tattcaaact ggactttata tcggtgggac
     8461 tttgcaatta atgacactag gtattactag ttttggtggt gcatcagtac cggattatca
     8521 aacagcagca ttgatcggta cttatttagc aatctcaact aaccaggatg cttctatagg
     8581 aataacttta gcaataccgg tagcaatgtt aattgtacaa ctagatgttc taaagtggtc
     8641 aacaaacatt ttttttcaac aaaaggcaga aaaattcgca caaaaagaag attatcaaaa
     8701 attagagtgg atgcaatatt tggcatcaat taatacaagc tttgtatctg gaattcctgt
     8761 tttgttaact gtgatattag gcccaacgtt agtgggaagt gcaatcaatt atattcctga
     8821 atgggttacc ggtggtttaa aggtagcagg gggactatta cctgctgttg ggataggaat
     8881 gctacttaaa tatttaccta caaaagatta ctttgcctat ttgttagttg gtttcgtttt
     8941 ggcagtttat ttccaaatac caattttagg gatttcgtta cttggcgctg caatcgcact
     9001 aatcgtatat aaaaatagat caaataatac tcaagtagca ccagctgcaa caggaggttt
     9061 tgacgaagat gaataagaaa ctgacaaaaa tcgacaaaca agtaattttc cggtggattc
     9121 tcaatggtgc aacttcatta aattatgaaa aaatggaagg gttgggttat gcgtattcta
     9181 tgcttccttt tatcaaggac acatataaag atgatcctga aggaatgaaa caatccgttt
     9241 tagctcactt acaatttttt aacactacgc cgtatacagc gccttacatt attggtatga
     9301 atgttggaat tgaaaaagaa gaaaaaaata agtctttgga agcagtaagt gctctgaaaa
     9361 caggtctaat gggtccttta gcgggaattg gagatagtgt atttgtagtt attccatgga
     9421 ctatttttgg agcaattgca gctaatatgg cattggaagg tagttttgtt ggaattattt
     9481 tgtggatact ggcatcaatt ttattaagga ttactgctta tcctttatac cttgctggga
     9541 aagaatcggg tgcgagccta ctaactcaaa ttgaaacaaa attaaagtct attactgatg
     9601 cagtatcaat tttagggtta atggtggtcg gtgcactcat tgccacagtt gtaaaagtga
     9661 atgttggctt tgaatttaaa caaggcgact taacaatgac cggaaatgaa atctttgatc
     9721 aagttatgcc tggtttatta cctgcatgtg tagtagcaat tgtgtattat ttgttaggac
     9781 gaaaggttaa accagtttat gtaattctat taatcttagt agtttctatt gcgttaagag
     9841 cgttaaatat tttgaagtag gtgttgaata tggatgaaaa aatgaaaggt attatacatg
     9901 ttcgtattga tgatcgaatg attcatggac aagtagcaac acaatggaca aatcaattga
     9961 gtgctaacag aattatggtt attaataatg aagtagccaa tgatgacgta aaaaaagctg
    10021 tagttcgcct cgctgcacca cctaatgtta gaacttcaat aattacaagg gaagtagcag
    10081 ttaaaaatat tctttctggt aaatatgaag gacaaaaagt tcttattgtt gcagtttctc
    10141 caatcgatgt taaatttttg attgataacg gtttaccaat aacaagtgta aacgtaggaa
    10201 atttatcaag aaggaatgga acagaacgca tccgtccgac gataaatatt actgaagaag
    10261 agagagaggc ttttaaagaa ctaattagtg atggagttga agtgacagtg atacaaactc
    10321 ctagagattc aaaagaattt ttaaaggatt ttatttatta agaacagagg atgagtggat
    10381 ggagtttata attacatctc atggaaatat tgccattgga tttgtagata cattaaaaat
    10441 gttttttggc gataatttaa attgtcatgt ggtaacactt ggagatgcag gcattgaaga
    10501 attccgtgaa aacattgaaa aaatagtttc tcaagtaaaa aatcaagaag ttatggtatt
    10561 tgctgacttg attggtggaa cacctttcaa cgaatttgct aaaagaagtg aagtatttca
    10621 aaatgggttt caacttcttg gtggcatgaa tttaggaatt ttgattgagg ctgtcaattt
    10681 gagattacaa ggaaaaaaaa gcgatgatat catcgaaaat ctaagaaata tgaatacaat
    10741 tgcgtgtctt gcagatatgc aaagacatac taacgaagag gatgagtgat aaatgcaaaa
    10801 aattttttac aagcctaaag atttttggtt tggggattgc atgccttttt ataaagatgg
    10861 aactttttat ctatttcatc aacgagatac gagaaatcct aaacctttaa ctgatccttt
    10921 tgggtggtca ctagttacaa ccactgattt tgtgaaattt gaagaatacg gggaagtaat
    10981 tcatcgtggt ggcaacgatg acattgatca atacatttac gctggaagta ttgtccaagg
    11041 aatcaatgag tatttagctt tgtatactgg acataatcgc aaagctaagt tggatggaaa
    11101 aacttcagaa gtgttaatga ttgcaagttc taaagattta attcattgga aaaaggatgg
    11161 cgaagcagca aaacttgttc ctcaagaagg atatgataag aatgattggc gcgatccttt
    11221 tgcaattttt gatcaagatc atggaaaata cgttttgata ctaggagcta gaaaaccagc
    11281 aagtaagaaa aagccgacgg ggagattggt ctattttgaa tccgaagatt taaaagattg
    11341 gaatttcaaa ggagactact gggctaataa tgaatttaac atgattgaaa tgcctgatat
    11401 atttgaaatt gatggaacgt ggtatttact tttttcagag tattgtgaag ataaaaagac
    11461 taaatatcga gtcggagcag gattattttc agattggaaa tcaccagtag acgaagcttt
    11521 cgacggaaaa gcctattatg cagcaagaac agtcggaact aatgaaactg gccgttttct
    11581 aactggctgg gtggcaacaa aagaagggga gaacgatcaa aataactggg attggggcgg
    11641 tgcacttgtt ccacatcaaa ttattcaaaa gacagacaaa actcttggtg ttaaactgcc
    11701 tgaaaaaata attgagaatt ttaaagaaat cactccattt tcaagtttta agttagagaa
    11761 agaatttgga aaaaaagaaa gagtaattgt tcctaaaatc gaagattatt ttctatttga
    11821 aacaactttg actattacat cgttaacaaa ggagtttgct ttaaaattat ttgtagatag
    11881 tgatactcaa gaaggatacg aatttcgtat ttcattgatt aataatcagc ttactttcgg
    11941 aaaaactccg aactatcctt ggcctcaaat gtttgataag gggctgcaac gaccactatt
    12001 tttggaaata ggcaaagaat atgaactaaa gttaattgtt gatgatacga tagctgtttt
    12061 atatattgat aatgttgctt taagtactag aatgtatgat aaagtcggaa cagcattaag
    12121 tatgactgtt acggagggag caatcaaggt agataaacta gactattcaa taaattatga
    12181 atgtggattt taaaacaatt gaagggatga tgtaagtgaa attaaatggt ttacaacata
    12241 ttggaattcc aacaaaagat tttggagctt caaaaatctt ttatgaatcc ttagggttta
    12301 gtctgattaa tgaagaaatg aatgtgggaa gtaaagtcgg ctttttcgag ttgaatggaa
    12361 cgataataga aatatgggaa gataaaacta ctgagtgtag aggagccatt gatcatctag
    12421 cattagatac ggatgacata gatgaggtat tcaattcaat tcaagattta ggatatgaat
    12481 tattagataa aaatattatg gaattaccgt tctgggaaaa tggaatccgt tactttaatt
    12541 tctacggacc aaatcatgag attatagaga taagccaaat gaagaaataa atagataacg
    12601 aagtgctcta agttggtatg aacgaaaagt acttcatttt ttggtatcag catttcaata
    12661 gtttaatgag aatttactat ttcaaattaa ttcaaagtga cagtattttt tacttatttg
    12721 gtttcaaatt ttgtaatcct tcctatttat tggaatacac tccacatttt gtatacaagt
    12781 catatcatgt tttagcaaag ctcttaccaa gcaaagttat aatgatgttt tccaaagttt
    12841 gatctttcga tggaggctaa cacatatttt ttgcaggaca gccaaaatta gatgtataga
    12901 atagagtctg tgaggcgtat agacagttta ttgtctgtat gccttttttt attttgaaaa
    12961 atttttagta tttttcgaaa aatgctcact cctag
//
LOCUS       DACCYH010000082        12927 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.086, whole genome shotgun sequence.
ACCESSION   DACCYH010000082 DACCYH010000000
VERSION     DACCYH010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.086"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            126..3653
                     /locus_tag="IVY85_002233"
     CDS             126..3653
                     /locus_tag="IVY85_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8045236.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3650..7372
                     /gene="addA"
                     /locus_tag="IVY85_002234"
     CDS             3650..7372
                     /gene="addA"
                     /locus_tag="IVY85_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP8045237.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7441..7782
                     /locus_tag="IVY85_002235"
     CDS             7441..7782
                     /locus_tag="IVY85_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8045238.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8091..9134
                     /gene="pheS"
                     /locus_tag="IVY85_002236"
     CDS             8091..9134
                     /gene="pheS"
                     /locus_tag="IVY85_002236"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP8045239.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9139..11559
                     /locus_tag="IVY85_002237"
     CDS             9139..11559
                     /locus_tag="IVY85_002237"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP8045240.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11729..12739)
                     /gene="trpS"
                     /locus_tag="IVY85_002238"
     CDS             complement(11729..12739)
                     /gene="trpS"
                     /locus_tag="IVY85_002238"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP8045241.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4396 a         2246 c         2739 g         3546 t
ORIGIN      
        1 gtatacttaa aaaagtagaa atatcagaac cttaaaagga tataatttga agaatataga
       61 acaaatgttc agtatttttt gatataataa aggagcaccg tatcatgtgt tgaaggagga
      121 aaagcatgtc tttacaattt attcttggaa acgggaaaaa agatcaccgt caagtcttga
      181 tcgacgaagc atccgcttgg ctcgaccagg atgcaagtaa ccaagtattt ttccttgttc
      241 ccaactacag caaattcgaa caggaacaag aaattttagc ggagatgcga agaagaagcg
      301 gaaaaaaaac tttcagcaca acaaacgttc aagtatttag tttttatcgt ctggcttggt
      361 atttgctgca acagacaact cttcttacag gaaatgaatt atcagaatca ggatctgcaa
      421 tgatcttgcg acagatttta gaaaaaaatg aggaagaatt aacaatcttt cgcggagaaa
      481 tcaataaaag agggtttatt cgtcaattac aagaactcta cagtgaattg cgtacaggaa
      541 atattcaacc agaagacctg atgcttttat ttggtgattc acctaatgca gaagaccagc
      601 aacttaaaat gaaagacttg aagctgattt tttcagctta tgacagcgaa ttgactcaac
      661 gtgcactaac aaatgaagat gcactatcta tactagcaaa ttatatgcag acacaagatt
      721 tcaacaacgt caaatttatt gtcagtggtt tttctcgaat caatggtcaa gagtatcaat
      781 tactgcaggc aatgatggaa aaggggcaat tggttgtcga tttattgctg gatcgtcctt
      841 atacagcgga tcttccagaa gtacttacgc tctttcatga gactggaaag ctttatttcc
      901 gtctttttca aagtgcaaga gaacggaaag tccctatatt agtggaccga tttgcaccag
      961 agcagactcg tccgacagag cttcaactgc tgcaaacaat gtgggaagag acatctagcc
     1021 aaaagaaagc ggcagtacac aaaatagggg gacaagagca tctccatttg tgggaagctg
     1081 aaaatccaac tgaagaagtc cgacaattag ctgttgaaat cagacgacta gtcagtgaag
     1141 aaaactaccg ttatcgtgat atccaagtgc tgacaaaaaa tatgtcttta tatggaaata
     1201 tccttcggcc gatctttcaa gaaatggcaa ttccttttta tatcgatgaa gaacaaatga
     1261 tggaaaatca tccgctgatc gaatggatca acagtctgtt cgctcttgac cgctattctt
     1321 atcggctaag tgatattatg cgttttttca aaactgaatt atttctgtcg acggaagcag
     1381 aaaccgaact tgatgaatgg aaacaagagc gagatgaatt caggaaaaaa ctggacatca
     1441 ctgaaaatgt agcacttgct tacaattatc aaggaactta ttggactcgt gaaaaagact
     1501 ggcagtttgt ggcgtatgat tttgaagcag aacagattga agacgtacag tatttggaag
     1561 aacagtcaaa tacgattaga cgattatttc agaaagctgt accagtattt ttgaaaaaaa
     1621 taaagaaaag caaaacagga caagaggccg caaatgtttt ttatcaattt ttgatccaaa
     1681 gtggcgtaca gcaacaatta ttgttttggc gtaatcagga agtagacaga ggaaacttag
     1741 aagcggcaag aaaccatgag cagacatggg gagcgctgat tgatctgttg gatgaatatg
     1801 tagtaattta tggtgaatcg gcttttgact ggtcactgtt ccaagaaatc attacaagtg
     1861 gtctagaaaa tctaagttat ggtaaaatcc ctaccgcaat cgaccaagtg cgaatcaatc
     1921 gtttggaatt agttcgacca aatcaagctg ccgtcacttt tgcattaggg ttgaatgatc
     1981 aagtgtttcc agaccgaaaa gagacaaaag gattgttgtc cagtgaagaa cgagaagtat
     2041 taaatcaggc cttgccagat ggacagtttt tattcgaccc atcaaaggaa agtatctctt
     2101 ttgagccttt ccaagcctat cttgtttttc tttccggaac agatcgatta tatcttagtt
     2161 atgcacaaag ctatgacaca gatggttcgc tgaagatgtc tccttatttg cgtagaatca
     2221 cagaatattt atctgttccc gtcgagagaa aagagcattt gacgatcgaa agcgacccta
     2281 attgttatgt agggacttac agaagcggaa tcaatacagt caataggatt tatcgattag
     2341 ctttagatga aaaaaaacct gtaccaaagc actggcaaaa gctcaaagaa ttagtactga
     2401 attcgtcatg gcgaaatttt gctttacaga tttttgaaag tcaggaacat ttaaatgttc
     2461 ctgtttcgat gccagaaaaa atggcggaag agctttatgg aaaagagatt tatacttcaa
     2521 tttcacgtat ggagaatttt tataattgtg aatacaaata cttcatgcaa tttggtttga
     2581 aattgaaaga acgaactgtt tatggtctaa ctccagcagc aactggtgat ttttatcatg
     2641 aatcattgga tcgtttcttc aagctactgt tttcaaacca attatcctta gtgaatatgt
     2701 ccgatgcgca gcgtagagac tttactgaaa aagtgctgca ggaagtattc ggagaacttc
     2761 ggtttgagat attggatagt tctgcaagaa tgaactatat ccgctaccag ttaggacaga
     2821 cgattcaaaa agtgacatgg gctttgcaaa aacagagcaa aaatacaggc atgcatcctc
     2881 ttcagacaga gattctattt ggtcagattg ctggagcaaa aggtatcaca ggtctggaat
     2941 taccgttgag taatggtgga aaactccatg ttcgagggaa aatcgatcga atcgatgtag
     3001 cttctgaaca agaagatact tggctaagcg tcgttgatta caaatctagc ggacgttcat
     3061 tcgatgtcac agaagcttat tatggcatgg ctatgcaatt attgacgtat ctggacgtag
     3121 cattgatgga tgcggtacgt ttgactggaa aggcggctgt taaaccagca ggatcatatt
     3181 atttacatgt tcataatcct gtattgacgg agacagatga tgtagagaag aatacattga
     3241 aaaaatttag ttatgatggc gtttttgtcg atgatcccgc tcttttagaa gtactagatc
     3301 attcactgga agcaaaagaa caatcactga tctatccagt gaaaaaaaat gcgaaagatg
     3361 aataccaaaa aggtagtttc agtaaagaca aatttttcac ggaagaagaa ctgcaactat
     3421 tcatggcgca taatcgggaa aatatgcggt tagctggaga tgcgattact tccggagaaa
     3481 tcaaactaaa tccagcttat aaagataaac agcggattgc ctgcgaattt tgtccattca
     3541 gaagcatctg tacgtttgat gtgatgctga aagaaaataa ttaccaccgt ctggagaaaa
     3601 tagataagga agaagcactg cgtagaatat tgaaaaggag tgaagaagag tgaatcaaat
     3661 tcctttaaaa ccgcaaaatg aacgattcac agatgatcag tggcaagcga tttttgatca
     3721 aggagataat ctattagtct ctgcgtctgc cggatcaggt aaaactacag tgcttgttcg
     3781 aagagtgatt gaaaaactaa aaatgggttt tgatatcgat gaattattga ttgtgacatt
     3841 tactgaagct gccgcaagag aaatgaaaga aagaattcaa gaggcattac aagaatccgt
     3901 caatagcgaa agtgaccctg tacgtagaca gcattttact aagcagttag ttttattgcc
     3961 cacagcgaat atctcaacct tacatgcttt ttgcctgaca gttattcgac gttattatta
     4021 cttaattgat attgatccag tctttcgcat gttaacagat gaaacagaaa cgatcttgat
     4081 gaaggaggat gtgtgggacg agctgagaga agcactttat gcagaaaatg atgaacgatt
     4141 tttccaatta acaatgaatt tttctaatga tcgttcagat gacggcttga cgaatctggt
     4201 gttttctctt tatgagtttg cccgtgccaa ccctgacccc caaaaatggc tagaacagtt
     4261 aagcgataat tatcgtttgc ctgaggggct agcgaaatct cgattatatc aaaaacaaat
     4321 ccgtccttta gttttggcag atatatacca atgtgtacag ttatatgaac agatgactca
     4381 gttagcacaa ggtgaaggat tagaaaaaat gcatgagcaa gtagctggag aacaacagca
     4441 gatcaagaat atctatgaag cgttctcgca agatcggtta gaagaagctt atgctggttt
     4501 ggaacaacta actttttcaa cttttaagag cagtcggaaa gcagaattga aagaaatttc
     4561 caatgaagtc aagggcatga gggataaagc aaaaaaactg attcagcaga taagtaaaag
     4621 ttattttcct gtttccccaa gccaaatgga agaattaacg gacaaagcac taccgttagt
     4681 cgaagaaatg acaaaagtca cgcaaagctt catggacggt ttcagtatgc gtaaacggga
     4741 aaaaggtgtg ctggatttca atgatctgga acatttagcg ttgcagattc tcacagaaaa
     4801 aacgaaagat gtatggctgc caagtgaagc ttcaaaacat tatcgaaaaa aattcaaaga
     4861 agtaatggta gatgaatatc aagacgtgaa ccaattacaa gaagccattt tatactggct
     4921 aagagaaccc gatgatacaa aaggaaatat gttcatggtt ggggatgtca aacaatccat
     4981 ctattctttc cgtttagcag atcctagttt atttatcggg aaatacgaga atttctcaaa
     5041 gaaagaaggc ggacgtcgaa tcgttttggc tgagaacttc cgctctagaa aagaagtcct
     5101 ttcttttacg aatcttattt ttgaacaact aatggatcca gcagtgggtc aaatcaatta
     5161 tgacgaagca gccaaactga tccaaggatt ttcagacttt ccagagaatg aacagtttga
     5221 gccggaaatc atgatctatg aaaaagaaca ggaagaatca gagattgaga ttccgacgga
     5281 tgatatactg gaagacaaaa cagaaggcga actttttatg acaggactaa aaattcgtca
     5341 attaattgat tcctccttta tgatttatga taaaaaatca aaaaaaagtc gtccgataga
     5401 gtacaaagac attgttctct tgacacctac aaagaagaac aacttaacga ttttagagat
     5461 tttcaaaaca ttggatattc cattagagat gaatgatgct caaaactatt ttcaagcgac
     5521 agaaattcgc acgatgatct cgctgctaca attaatcgat aatccctatc aagatattcc
     5581 gcttgcggct gtattgcgct caccgatcgt gggtctgatt gaaccagaat tagccagcat
     5641 tcgattagct aaccgtgcac atacttacta tgatgctgtg cttgcttatc aagcatcaaa
     5701 cgaagatgaa ttagcagcta agctggaaca ttttggcaag caactagagc attggcgaga
     5761 acttgctcgc cgttcttcga tcactgactt gttatgggat atctattatg agacgggata
     5821 tctagagtat gtggtcggtc tgccagcagg ggcacagaga caagccaatc tttatgcatt
     5881 ggttgatcgt gccaaagctt acgagcaaag ttctttcaga ggattatacc agttcgtgcg
     5941 tttcattgaa aaaatgcaag aaaaagataa agacttagca gaaccagtca tttcaataga
     6001 agataatgct gttcgcgtta tgacgatcca tgcaagtaaa ggcttggaat tcccagttgt
     6061 atttttgtta gatatgacaa aggagttcaa cttacaagac ttgcgcaatc ggtatgcttt
     6121 tgaagaaaaa cttggagcag gaattcgcta tatggatcct gaaacacgag tgttatatga
     6181 tacgttaccg tttcaagcca tcaaattggc aaagcaaaac aaattgttgt cggaagaaat
     6241 gcgtaaattg tatgtaggat tgactcgggc agaacaaaag cttttcattg tcggttccta
     6301 taaaaataaa gagcaaatga tccaaacgtg gtctgaagca gctgatcatg aggagttagt
     6361 ttttgatcct gcgctacgtt tgaagggcag aagcagcttg atgaattgga ttggttacgg
     6421 attgatccgt catcctgaaa tgcagaaata cttagaggaa gaaatttcga cttctcttct
     6481 tcaacattca aatgcacagt tttctatttc atggatgaat cagcaatcga tcattgaaca
     6541 aagacagttg cttgctgaaa aagaactggt taacttagat cagcagatga aagaagatga
     6601 aactttatta gcagattcgt tacaaaaacg tctggcatat gaatatcctt atcaagcttc
     6661 ttcacaaacc acaagttatc aatctgtatc tgaaatcaaa cgattattcg aagatccaga
     6721 tgatacacaa gaatcacgtt tgactctgga aagcagtcag aacaaagcag catcccgtca
     6781 atttcgttat acgcaagaac aactagcaga accaaaattt ttgcaaaaag atcggcaagt
     6841 gtcagcagct gctgttggaa ctgctacgca tgctttgtta caattactgc cattagagat
     6901 gccaactaca gagagtatcc atcaaaaatt acaggaattg gtaaggaaac ggttagtaga
     6961 cgaaaaagtc gcgaaaaaag tagatgtttc ttctattata tggttctttc aaacagaact
     7021 tggtcaacag ctgattgcca acaaagaaaa cgtaaaacga gaacaacctt tttccatgct
     7081 gcttcctgct gacgaagtat ttcaagacta tccaaatcaa gaagatgaac tattaatcca
     7141 cggaatcgtc gatggatacc tagaagaaaa agatcatctc aatatctatg attttaaaac
     7201 agattttatt ttacatccgg atgatccagc agaaatagat gcaatcgtcc aaaaatatca
     7261 aggacagcta agactttacc aacaagcaat gtcagaagct ttgaataagc cagtagaaaa
     7321 tgttttccta attttgcttc gagtgaaaca aattataaac attaacaaat aattataatt
     7381 gagcaaactt gacttatgtt ttgtaaggat tatgctatac tctgggaaga ggtgagtatg
     7441 atggagctaa cacagacaga attcgtttta tgccctaaat ttgaaaaaac attctctatt
     7501 ttaggaaaaa aatggaacgg attgattatc gatgtactac tcgaaaacgg tccgcaacgc
     7561 tttgttgagc ttgcggcaaa aatcccgatg gtcagtgatc gagtattagt cgaacgcttg
     7621 aaggaattag aaaaagaagg aatagtcact cgaaactttg cctgtagcga aaagagccgt
     7681 tcagaatatc tcttgactga aaaaggagca gacttgcaaa aagcgatgcg tcaaatacaa
     7741 gtttggagcg aaaaatggat cacagaggaa gaatgcggtt gattttgtaa aaaaaatctt
     7801 gtaaactata gtccatacga ataagtaaag aacgatgacg aaaaagagta gtttggctaa
     7861 accagttcag ggagatctgt cgcagactga aagcagatcc tgtgtttgaa aaatgaagtt
     7921 cactttccga gttcgtctga taaaatcaga ccggcaattg tcgttaacga agtaagagtg
     7981 cggtgtaagt catttatccg caaattagga tggtaacgcg aagacctcgt tccttaacag
     8041 gaatgaggtc tttttgtttt gtatttggaa tttaaaggag gaacaagcac atgtctctac
     8101 aagcacaatt agagagcttg aaagaagcaa cgctcgtcaa aatcacagcg gcacaagatt
     8161 taaatgaatt aaatcaaata cgtgttgaaa cgttaggaaa aaaaggaccg atcacagaag
     8221 tattacgtgg gatgaaagat ctaagtccag aagagcgacc aaaagtcgga agtttcgcaa
     8281 atgagatccg agatcttttg acagaagaga tcgagcgaaa aaaacaagta ttagaagaag
     8341 aagcgttaaa tgcagctttg gcaaaagaaa caatagacgt gacactacct ggtagaaaag
     8401 taaaacatgg tacacgtcat atcgtgacac aaatcatgga agaaattgaa gatatttttg
     8461 tgggtatggg ctatcagatc gtagaaggat atgaagtaga atctgatcat tacaatttcg
     8521 aacggatgaa tctgccaaaa gatcatccag caagggacat gcaagatact ttctatattt
     8581 cagacgagat attgattcgg acacatactt caccagtcca agcacggaca atggaaaaac
     8641 atgatttctc taagggtgct ctacgaatga tctcgccagg gaaagtcttc cgcagagata
     8701 cagatgatgc gacccacagt catcagttcc atcaaattga agggctagtt gttgataaaa
     8761 acatcacaat gggcgacctt aaaggaacat tagaagtcgt aatgaaaaaa atgtttgggg
     8821 aagatcgtga aattcgtttg cgtccaagtt atttcccatt tacggaacca tcagtagaag
     8881 tagatgtcag ctgtttcaaa tgtggtggtg ccggttgtaa cgtatgtaaa tacactggat
     8941 ggatcgaaat tttaggagct ggcatggtac acccgaatgt gttgaagatg tcaggaatcg
     9001 atccagaaga atattcaggc tttgctttcg gccttggacc agaccgcgta gctatgttgc
     9061 ggtatggagt gaatgatatc cgtaatttct atcaaaatga cttacgtttc ttaagtcagt
     9121 tcaaggggga gtaagaaaat gttagtttca tataaatggc tcaatcgtta tgtagattta
     9181 tcgaatgtaa caccaaaaga attagcggat aagatgtctg tgacaggaat cgaagtagag
     9241 ggagtaacgg ttccagaaga aggcttgaaa aaaatcgttg tcggtgaagt caaggaatgc
     9301 gtaccacatc cagattctga tcacctttct atttgtcaag tcgatattgg cgaagacgaa
     9361 ctgtctcaaa tcgtttgtgg agcgcctaat gtgaaggttg gaatcaaagt gattgttgca
     9421 cttcctaact cacgtatcgc tggtaatgta aaaatcaaaa aaggaaaaat gcgtggccaa
     9481 gtatcaaatg gcatgatttg ctcattacaa gaaattggct attcagatag tgttataccg
     9541 aaagaatacg ctgaaggtat ctattatatg ccacaagaag ctgtaaatgg cgatccagtt
     9601 tttccttatt tggatatgga tgattcgatt attgaattat ctatcacacc gaatcgtgcg
     9661 gatgcattgt caatgcgtgg cgtagcttat gaagttgggg caatttatcg tcagactcct
     9721 acattcgatg acgaaaagct ggtagaatcc tcacgtcctg ctagcgataa aattcaagtg
     9781 gaagttgaag ataaagaagc agttcctgct tatcaaatca gaatcattga aggagtaaga
     9841 atccagccaa gtcctcaatg gctgcaaaat cttttgatga acgaaggcat tcgtccaatc
     9901 aacaatgtag tagatgtgac gaactatatt ttgcttttat tcggacaacc actacatgca
     9961 tttgactacg acaaattgaa atcaaaagag atctttgtcc gccgtgcaaa agaaaatgaa
    10021 acattagtaa cactagatgg agaatctcgg gaattgacaa ctgacaatct cgtcattact
    10081 aacggcaaag aaccagttgc tttagctggt gtcatgggag ggttagactc agagatcacg
    10141 aatgaaacaa caacagttgc actagaatct gctttatttg atccaatttc gattcgtcgt
    10201 acagcaaaac aatttaattt gcgtagtgaa tcctctgcac gttttgaaaa aggaatcaac
    10261 catgcgactg ttggagaagc cagtgaggtt gctgcagcaa tgatcgtttc tcttgcgggt
    10321 ggggaagttc tacaaggcgc ggtcaaaggc tcagagatca aagcagaaga cgtatcagtg
    10381 aagatttcac ttgaacgcat caatcaatat ttaggaacat ctcttgtcgt taaagaagta
    10441 aatgagattt ttgaagcact tggatttagc tatgaagaaa aggagtatgt ttattccgta
    10501 gtagtaccac caagacgctg ggatatccaa atcgaagcag atattatcga agaagttgct
    10561 cgtatttatg gatacgacca gttgccatct acacttccaa gtggagaaac ggttgctgga
    10621 agcttgtcaa aagaacaaga aactactcgt aaaattcgta caatctttga aggaagcgga
    10681 ttgagcgaag caatcagtta tgcattgaca acagaagaaa aatcaagaca gtttgttata
    10741 aatgaaagta atattacacg tttggactgg ccaatgagcg aagaacgttc tgtattacga
    10801 atgaatttga tcagcggact attagatgat atcagttata acactgcccg aaaaaataca
    10861 gagattgctt tatatgaaat tggtcgggtg ttcttccaag aaaatgatcc acgtacacat
    10921 ctgccgcaag aagtaaaaca tgcagcaatc gctatttctg gtatttggga agaaaaagat
    10981 tggcagacga aagctcaaaa tgtagacttt ttcaccatca agggtctttt agaaaactta
    11041 tttgaacaac taggcatcac tgaagatgtc cattatcaaa cagtgacaga aatgaaggaa
    11101 atgcatcctg gaagaactgc tgcgatttac cttggcgaaa aatttattgg ttttgtcggt
    11161 caagtgcatc caacgattac aaaaagttat gatattccag aaacatatgt tgctgagttt
    11221 gatttatctg ctatagtgga agctgctcaa aaagggatcg ttttcgaagc agtgacaaaa
    11281 ttcccagcag tcagccggga catcgctttg cttgtaaaag aaactgtgac aaatcaggaa
    11341 ttaacagaag ttatccgttc tgctgccgga agatttttaa cagatatcca attatttgat
    11401 gtctatcaag gagaaaacat tgaaaaagga cataaatcca tggcgtatag cctgacattc
    11461 gtcaacccag aagcaacatt gacagatgaa gaaatcaata aagcaatgga aaaagtcgaa
    11521 aaagcgttgg tagaaaacct agaggcaagt attcgctaag aaggaaaata tagtaagtaa
    11581 cgatcaagta gcttttgaag accatacatt tgaaaaaaag atgtgacaca acttcgtcac
    11641 atctcttttt ttgattttaa aaaaggaata gcacagagac gagataaaaa aacaggcctt
    11701 tacagcctgc ttttttagag taaatgaatt aatggaagta attgattccc attgctgatt
    11761 ttacttcaga aagtgtttgt gcagctactt tagcagcttc ttcactacct ttacgtaaga
    11821 tttccataac agctgaagga tctttttcca attcttctcg gcgcgcacgg atcggtgcaa
    11881 attcagcttc caatacatca atgaggtatc gtttgatctt tacatcacca agtccgccgc
    11941 ggcggtaatg agctttcatt tcttcaattg cttctttatc tgttccgaat acatccaagt
    12001 aggtgaagac catattccct tctacttgtc caggatcttg tacatggatg tggtttggat
    12061 cagtatacat actcatgact tttttctgta agacatcggc aggatcggag atataaatac
    12121 cgtttcctaa cgatttactc attttgccat ttccatcgat tccaggtaat ctacccattc
    12181 cttttggtgg gaatacgcct tttggttcca ccaagacttc accatatgta tgattgaagc
    12241 tttgtacgat ttcctgtgtc tgctctagca ttggtttttg atcctcacca acaggtacaa
    12301 gattcgcttt gaatgcagtg atatctgctg cttgagaaac aggatagata aagaatcctg
    12361 taggcacgct ttcaccgaat tttttttgtt cgatttctgt tttgacagtc ggattgcgcc
    12421 gaacgcgtcc tacgctgact aagttcaaat aatacatggt taattcagct aattccggaa
    12481 tttgtgattg gataaataaa gtagattttg caggatctaa tcccacggct aaatagtcta
    12541 aagcaacttg caagacattt gatgaaacct tctcaggatt tttagcatta tctgtcagtg
    12601 cttgcatgtc agcaatcatg acaaacagtt ggttgtcttc atctgcctgc atagccactc
    12661 gggtttttag agaaccgacg taatgcccta aatgcagctt cccagttggt cgatctcccg
    12721 ttaaaataat atttttcaaa aaagacacct ctttacgatt tgtaagtctg agcacttaat
    12781 atcttaccgt ttttcctgaa aagaaacaac agttcctaag gaaaaaacaa ggaaagagga
    12841 taaaataact aatagattag aaatggaaga aatatttatg ggagaataaa cgttctaaga
    12901 aactttacaa attactaaac tatcaga
//
LOCUS       DACCYH010000083        12732 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.087, whole genome shotgun sequence.
ACCESSION   DACCYH010000083 DACCYH010000000
VERSION     DACCYH010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.087"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IVY85_002239"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IVY85_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8045242.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IVY85_002240"
     CDS             complement(322..891)
                     /locus_tag="IVY85_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP8045243.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IVY85_002241"
     CDS             1077..1979
                     /locus_tag="IVY85_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8045244.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IVY85_002242"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IVY85_002242"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP8045245.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IVY85_002243"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IVY85_002243"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP8045246.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IVY85_002244"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IVY85_002244"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP8045247.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IVY85_002245"
     CDS             8547..8732
                     /locus_tag="IVY85_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP8045248.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IVY85_002246"
     CDS             complement(8794..9990)
                     /locus_tag="IVY85_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8045249.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IVY85_002247"
     CDS             complement(10160..11596)
                     /locus_tag="IVY85_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8045250.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IVY85_002248"
     CDS             11889..12698
                     /locus_tag="IVY85_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8045251.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3801 a         2672 c         2250 g         4009 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt ga
//
LOCUS       DACCYH010000084        12692 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.088, whole genome shotgun sequence.
ACCESSION   DACCYH010000084 DACCYH010000000
VERSION     DACCYH010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.088"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(287..649)
                     /locus_tag="IVY85_002249"
     CDS             complement(287..649)
                     /locus_tag="IVY85_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045252.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="IVY85_002250"
     CDS             complement(723..1103)
                     /locus_tag="IVY85_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP8045253.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="IVY85_002251"
     CDS             1185..2360
                     /locus_tag="IVY85_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8045254.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="IVY85_002252"
     CDS             complement(2437..3021)
                     /locus_tag="IVY85_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8045255.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="IVY85_002253"
     CDS             3222..4220
                     /locus_tag="IVY85_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8045256.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="IVY85_002254"
     CDS             4332..4841
                     /locus_tag="IVY85_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP8045257.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="IVY85_002255"
     CDS             complement(4871..5530)
                     /locus_tag="IVY85_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP8045258.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="IVY85_002256"
     CDS             complement(5532..6296)
                     /locus_tag="IVY85_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP8045259.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="IVY85_002257"
     CDS             complement(6304..6981)
                     /locus_tag="IVY85_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP8045260.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="IVY85_002258"
     CDS             complement(7018..8403)
                     /locus_tag="IVY85_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP8045261.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSCLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="IVY85_002259"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IVY85_002259"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="IVY85_002260"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="IVY85_002260"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP8045262.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9341..10198
                     /locus_tag="IVY85_002261"
     CDS             9341..10198
                     /locus_tag="IVY85_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP8045263.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10279..10614)
                     /locus_tag="IVY85_002262"
     CDS             complement(10279..10614)
                     /locus_tag="IVY85_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045264.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10681..12051)
                     /locus_tag="IVY85_002263"
     CDS             complement(10681..12051)
                     /locus_tag="IVY85_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP8045265.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12129..12665)
                     /locus_tag="IVY85_002264"
     CDS             complement(12129..12665)
                     /locus_tag="IVY85_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8045266.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3770 a         2578 c         2118 g         4226 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgatatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga caactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 ctttttttct atggtaaaat aaatacaaga ggtgaacaag atgaaaatgg ataaattact
     9361 tgctctttac ccacaagcac agaaaaaaaa ctctccagca tcagaagcgg atatcctttc
     9421 ttttgcaatt gatgatgctt ttatctggat caaacaagat tcattatctc aacaagaaac
     9481 cagccttttg aaagcgttat tccctgtaat caacgatcaa aaaaaacacc catggtatca
     9541 ctatcttttt aaagatgctt cttgtgttca agaaaaaagc tttcgtttga tccagcttca
     9601 cgtcgaatca aaaggagaat tcttaaaaaa agaatggcaa gatactattt tggaattatt
     9661 tcgctattct gctgatttct ttttttatac agacacagat gcgctcttag tcgaacaaat
     9721 aaaccgtgat tatcttgatg ctactgaaat caacggtatt tttctatctt tagatgctga
     9781 ttttgatacg acaacgtctg tattcgttgg ttctttccat tcacctggca ccgacctcgt
     9841 ccctttgttt gctgaagaaa gaaaaatttt tttaacagag caaaacaatc tttatactca
     9901 cagccgtact ttttctatgc aagatgtggc tttacattac tatacaaaag atactgtaaa
     9961 agatagtaag ttgatcaaaa gttacataga actgatcaaa caaaatgaaa tggaagtgat
    10021 cattcaagaa ttatggaatg aattaggcaa tgtcagctct gctgcaaaaa cactgtttct
    10081 tcatcgtaat actctaaaat atcgaatcga aaaatttcaa gaacagtctg gtttcaatct
    10141 aaaaaaagcc aacgatttac tgttttgcta tcttttattg cttcatgaac ataattaaag
    10201 atagattaaa ggggttgtga caataatttt gtcacaaccc cttatttcga atgaatagtg
    10261 ttcaaaagct agtttctctt acatcatttt ttcaacaaaa tgcattagcc ggtcttttac
    10321 atcttcggtt gaatctttga ttgaatcacc ataaacagag attttctttt ttcctgattt
    10381 gatttttgct aacattccca tcaaagaatc caagtcacta tctgaaagac catctacgat
    10441 ttctagtaat ttttcatttc catcaaattt gtcatagacg aattttttta ctttcgtccg
    10501 agtagtcata tgatggatct tttccattac ttttcccgat acaacgactg ctgtcgctac
    10561 actggcagcg gctgctacgc ttaatccgat tgttactttt gttgatgttt tcataagtat
    10621 cagctccttt ttatctatct ccatcatagc atagacaaaa aaataaacga actaatccac
    10681 tcagtgaaat ctcaatcctt tagggaaaaa gttctttact gttcctttta cttgtttccc
    10741 aaatccgaca ggtatcttat ctaatactag caaaacccat ccttgattcc catcacgttg
    10801 aaatgtttct cctgctgtgt aagattgcca ttccttttga gtgatcggca agcaaggtat
    10861 attctctatc ttttttgtag caagcgctaa tgcatagcta ggttcaaatc gattcttttt
    10921 gaaatctcct aaatgcaaac ctgtacgtac gacttttaaa ccatcgagag aaggggctaa
    10981 ctcaggaact tcccaaagat ggtcgttgaa cactaaaagt cttccagtag cttcataatg
    11041 aaaatcattt gaaaactccg tccagagttt ttcctgctct tttgtcattt gcactttact
    11101 tttttttcgt gtcttctttt ctttatgcat ctgattttgt ccatgaaaag tcagctttgc
    11161 aacaaaatgt ccttcaccct gatctttatg aggccatata cgtattgttt tttccaaacc
    11221 agctacgctt ccccattcag aacgtcctga actaacggat tgagtcaaag gaatctcctc
    11281 aatcgttact ggataatttt ctacaagcca gctaatgatc tcttcatttt cttctggtgc
    11341 aaacgtacaa gtcgaataaa tcagttgtcc tttatttttt agcattttta ttgctgaaga
    11401 caaaatctct tgctgacgtt tttgacagta tagcggagat tcttctgtcc actcttttat
    11461 tgcatttgga tcttttcgga acattccttc tcctgaacaa ggagcatcaa ccacgatccg
    11521 atcaaaaaag cccgaaaaat gagggacaag ttctgcgggt gcatgatttg tcactattgc
    11581 gttcgaaact ccccatcgtt ctatattttc tgataaaatc ttcgcacgtt tcggaaagat
    11641 ctcattcgtc actaataacc ctttcccctt catttgagca gctaattggg ttgatttacc
    11701 accgggagcc gcacacaaat ccaaaacttt ttcaccgggt tttgctgcag cagctgttcc
    11761 aacgatcatt gcacttggtt cttggctata ttcataccca gcttgatgga ggaaagactt
    11821 tccattgaca gtgccaagaa atccctcatt gctgtaggga gcgggttgta attcttcgct
    11881 atggtaagtt tggaccatgt ccaatcctgc cggttttagt ggattccacc gaaatccttt
    11941 tttcacgctt ccttgttcaa gcgcagaaaa aaaatcggat gcttcttcgc ctaaaagaag
    12001 acgatatttt ttgataaatt gctgtggtaa cgttgtcgct tcctccttca ttccaatgtt
    12061 tcgttagaca ttttacaatc ttttttacga aaaataaagg tggctgatct actttttcat
    12121 gttgctgcct aaggtgactg ttcctttaag atattttcca agtattcatc cgtcacctcg
    12181 tttttatccc aaacagcgtt catcatttca agaaaatctt gagaagaatc atctgagcgc
    12241 aaacttccca atgcattaag ccataccaat tcaagcgact tttcttcttt gatcttcgtg
    12301 tgacctttga catatgagaa aaatggccgc ttaaatagcg aatccatgtc taaatcagat
    12361 tttgcaatga tttcacgaat actttcatgg atagaaaaaa aatctttcaa taagtagaca
    12421 atgcttaaaa catcatcgga actgcattca gctaagtagt tttcaaaata aacagtgatt
    12481 tcttctaata tcttctcttt taacccgctg attgatttga aatgtgtata aatcgggtat
    12541 gtagaacaac cgcagaactc tgctaactct cttgcagtaa ttgacttcat tccatattct
    12601 tccacgtata ccatactttt gaacagcaaa tattctttac tataatttgt tactctcccc
    12661 atcattctta cctccgtctc ttttcatcta cc
//
LOCUS       DACCYH010000085        12536 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.089, whole genome shotgun sequence.
ACCESSION   DACCYH010000085 DACCYH010000000
VERSION     DACCYH010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.089"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            34..771
                     /locus_tag="IVY85_002265"
     CDS             34..771
                     /locus_tag="IVY85_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8045267.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            785..1600
                     /locus_tag="IVY85_002266"
     CDS             785..1600
                     /locus_tag="IVY85_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8045268.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1613..2260
                     /locus_tag="IVY85_002267"
     CDS             1613..2260
                     /locus_tag="IVY85_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8045269.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2276..2932
                     /locus_tag="IVY85_002268"
     CDS             2276..2932
                     /locus_tag="IVY85_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8045270.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3094..3915
                     /gene="racE"
                     /locus_tag="IVY85_002269"
     CDS             3094..3915
                     /gene="racE"
                     /locus_tag="IVY85_002269"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP8045271.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3918..5273
                     /gene="rph"
                     /locus_tag="IVY85_002270"
     CDS             3918..5273
                     /gene="rph"
                     /locus_tag="IVY85_002270"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP8045272.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5274..5792
                     /locus_tag="IVY85_002271"
     CDS             5274..5792
                     /locus_tag="IVY85_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP8045273.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5899..6399
                     /locus_tag="IVY85_002272"
     CDS             5899..6399
                     /locus_tag="IVY85_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8045274.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(6679..7599)
                     /locus_tag="IVY85_002273"
     CDS             complement(6679..7599)
                     /locus_tag="IVY85_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP8045275.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            7764..8777
                     /locus_tag="IVY85_002274"
     CDS             7764..8777
                     /locus_tag="IVY85_002274"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP8045276.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(8887..9642)
                     /gene="fabI"
                     /locus_tag="IVY85_002275"
     CDS             complement(8887..9642)
                     /gene="fabI"
                     /locus_tag="IVY85_002275"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP8045277.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            9862..10302
                     /gene="fabZ"
                     /locus_tag="IVY85_002276"
     CDS             9862..10302
                     /gene="fabZ"
                     /locus_tag="IVY85_002276"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8045278.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(10417..11178)
                     /locus_tag="IVY85_002277"
     CDS             complement(10417..11178)
                     /locus_tag="IVY85_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8045279.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            11367..12461
                     /locus_tag="IVY85_002278"
     CDS             11367..12461
                     /locus_tag="IVY85_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8045280.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         4039 a         2175 c         2642 g         3680 t
ORIGIN      
        1 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
       61 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
      121 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
      181 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
      241 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
      301 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
      361 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
      421 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
      481 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
      541 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
      601 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
      661 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
      721 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
      781 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
      841 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
      901 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
      961 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
     1021 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
     1081 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
     1141 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
     1201 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
     1261 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
     1321 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
     1381 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
     1441 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
     1501 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
     1561 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
     1621 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
     1681 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
     1741 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
     1801 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
     1861 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
     1921 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
     1981 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
     2041 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
     2101 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
     2161 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
     2221 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
     2281 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
     2341 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
     2401 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
     2461 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
     2521 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
     2581 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
     2641 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
     2701 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
     2761 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
     2821 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
     2881 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
     2941 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
     3001 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
     3061 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
     3121 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
     3181 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
     3241 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
     3301 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
     3361 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
     3421 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
     3481 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
     3541 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
     3601 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
     3661 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
     3721 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
     3781 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
     3841 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
     3901 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
     3961 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
     4021 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
     4081 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
     4141 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
     4201 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
     4261 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
     4321 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
     4381 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
     4441 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
     4501 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
     4561 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
     4621 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
     4681 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
     4741 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
     4801 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
     4861 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
     4921 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
     4981 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
     5041 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
     5101 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
     5161 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
     5221 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
     5281 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
     5341 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
     5401 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
     5461 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
     5521 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
     5581 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
     5641 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
     5701 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
     5761 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
     5821 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
     5881 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
     5941 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
     6001 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
     6061 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
     6121 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
     6181 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
     6241 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
     6301 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
     6361 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
     6421 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
     6481 gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaattg cttttcaaat
     6541 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt
     6601 ttagagcgtg ggacaaaagt gttctttact tttgtcccac gctcttttta gtgagattcg
     6661 ttgcatcgtt tgtccaaatt attttccgac agcaattgga ctgttcggaa tcgtgaaacc
     6721 agaagctgtc aattctttga cataggagct caagaactct tcttttaagg catattgttt
     6781 tccgttcaat gcgtagcaga ccgtacgaat cgcaaaattg ctgtttccaa gatccactaa
     6841 tccaaagata gaaggttcag tttggagctc ttcttggtat ttttcagcta atgtacgatt
     6901 tactcgatca atgatctcat agattttgtc atatccttct tcaggaacaa tccgaatatc
     6961 taaaagtact tgcatatttg cacgagagag attgctgatc gtggtgatgt ttcgattcgg
     7021 gatgaaatgg acagtaccat ccgttgcttt cagctgaagg gtacggatac caacagatac
     7081 gacggttcct tcaatcgtta agtttgctag cttgatgtag tccccaacat ccatctgttg
     7141 ttccatgata ataaaaaatc cggtaataac atcactcatg aacccttggg caccaagacc
     7201 gattgccaca cctgcaattc cggcaccagc caaaagtgat ccgatcggca caccgatagc
     7261 agataaaatt gcatagataa aaaagaaacc aagggtataa tgaaaaatag tatttaaaag
     7321 actatgtaaa gtctttaagc ggctctcgtt tagaatggct tttttagaat actgattaaa
     7381 agacttatca atcagatatt ttcccacacg gtgaagaatc caaaaaagga cgatcaaaaa
     7441 aatcagatat aaactttttt ggataaaggc agccaaaatg gcgtcccaat caatcccgtc
     7501 ccaaaaccgc tgaaacgcat ttactttgct gacagcttga tctgtcaatg tctcgccaga
     7561 agagtctgtc gtaccggcag ttgctaataa tgaaaacata aaatcctctt ttctaaaatt
     7621 tgattctttc tgtattgtac caagaagttt tcaaaaaagg aaatagcaaa aataactata
     7681 atgaaggaaa aaagctgaaa ataaaatgac aaataatatg gataatgata aaatgtggag
     7741 aaaataacga aggtggtaag tatatgcaaa tcgattggaa caatctaggt ttttcttata
     7801 tcaaaactcc ttggcgtttt attgccaaat ggaaagacgg acaatgggaa gaaggagaat
     7861 tgacagaaga taattacctg acgattcatg aaggctctcc agcgcttcat tatggtcagc
     7921 aatgttttga aggtctaaaa gcttatcgtc gaaaagacgg caaggtcaat ctctttcgac
     7981 ctgaacaaaa tagtcgacgg atgaatcaaa gtgctagacg attgctgatg ccaatggtcc
     8041 ctgaaaagat gttcgttgat gctgtcaagc aagtggtaaa agcaaatgaa gattttgtgc
     8101 cgccctatgg aacaggcgct acattatact tgcgcccttt actgattggg gtcggtgaaa
     8161 acattggtgt ccatccagct ccagaatatt tatttgtcgt cttctgtacg ccagtaggag
     8221 cgtatttcaa aggtggacta aaaccaacga attttattgt atctgattac gatcgtgctg
     8281 ctcctaaggg gactggtgca gcaaaagtcg gcggaaatta cgcagcgagt ttacttccag
     8341 gaacagaagc aaaagaactg gattactcag attgtgttta tcttgatcca gccacacata
     8401 cgaaaatcga agaagtaggt gcagcaaatt tctttggaat tacaaaagac ggaaaattca
     8461 tcactccgca atcgccctct atcttaccaa gtatcacgaa atattccttg ttgcagttag
     8521 ctgaagaacg actaggactg caagcaattg aaggagatat tttcattgat gatctagatc
     8581 aatttgcaga agctggtgca tgtggtacag cggctgttat ttctccaatc ggtgggattt
     8641 atcacaataa caatcttcat gtattttata gtgaaacgga agtaggacca gtaacaaaac
     8701 ggttgtatga tgaattgaca ggtattcaat ttggagacat agaagcacca gaaggctgga
     8761 ttgaagtcgt ggaataattt caaagtaatg agttgaaaaa aacgtaaaaa gaaaaaatca
     8821 ggaagagact ttaaaaagtt ttttcctgat ttttatatgt tttactttaa agaatgcaac
     8881 aaaagtttat gaaagatgga cacctttgtc aacgtagata atatcaccga tgattccgct
     8941 ggaaagtggg ctgattaaga aagcacaagc attaccgact tcttcgatcg tcacaccttg
     9001 ctgatcaggc gtacgagatt cagataactg gatcagtttt tgatagtctt taacacctgt
     9061 gacagctaat gttttgattg ccccagctga gattccattg acccgaattc ctttaggcga
     9121 gaactcagct gccaaataac ggatagcagc ttcaagagaa gctttcgcaa tacccatcat
     9181 gttataattc gggattgcgc gttctgaacc tagataagtc atgctaacga tcccgctgtt
     9241 tggtgcaaga aacggttgtg cgtattttga tacagcaatt aaagagtagc tgctgatatc
     9301 ttgagctaat tgatagccgt ctctggaaat atcagacaca tttccggaca attcttcttt
     9361 gttggcgtaa gcaactgcat ggacaaggcc gtggatctcc ccagcatatt caccgatggc
     9421 attcattgct ttttcgatgc tttcgtcgct agaaacatca cattctacag tgaatgcttc
     9481 ttcgccgacc aatttgacta atgatttttt cattcgttca ttttggtaag tatagataat
     9541 ttctgcccct tgtttcaaca ttgcttcagc acaaccccaa gcaatgcttt ttttgttagc
     9601 gacgcccata atgacgattt tttttccttc tagaaatgac ataatgactc tcctttgttt
     9661 ctgataaaaa tttgatagtc gtaaaaaaat agagaataat agctaaaata acttcttgat
     9721 ttcttaatta ttagtcaaaa tgttttgatt gtcaaactat tttttcttaa attaaaaaaa
     9781 gattgctaaa tatttaggaa tatgatactt tgatagtcaa agtaatgaaa atggcaattg
     9841 cctgaaagaa ggagtttaat tatgtcttac gtattatctg ctactgaagt aatggaattg
     9901 attcctaatc gttatcctat ttgttatatt gattatgtag atactttaga accaggaaaa
     9961 aagatcgttg cgacaaaaaa tgtaacaatc aatgaagatt ttttccaagg tcactttcct
    10021 gacaatccag tgatgccggg tgcgttgatt ctagaaacac tggcacaagc aggttcgata
    10081 cttatcctaa aatcagaaga gtttctagga aaaagagctt atatcggtgg gatcaataag
    10141 gccaaattcc gtcaaaaagt tgttccagga gatgtgctga aatgtcattt tgaaatcacc
    10201 aaaatgaagg gatcagtcgg tacagcgtta tctgcagcat atgtagatga taaaaaagtc
    10261 tgtgagtgcg agttcacgtt catcgtcaac caaaatgctt gactataaaa cctgatatag
    10321 caaaacaaaa ggaagcttac ttgcttttta tgtaaaaaga agacgcctga atcctagcat
    10381 acgttgtcat ctatgcagga ttcagacgtt atttttttat ttttgttttg cttcttgcag
    10441 tatattgatt ttatcgattt ttacttcttc ttttggtttg tctgaactat ctgtttcgat
    10501 atttgcaata gcatccacga catccattcc ttcaatcact tggccgaaga cagtataatg
    10561 gccatctagt gaaggataac cgccgttttt ataagcgtcg atgattgctt gaggatagtc
    10621 gtcttttaaa agaccatcat gcatatcatc tgtattttga acgataaaga actggctgcc
    10681 gttactgtta ggatcttgtg agcgagccat agataacgca cctcttatat tataaaggtg
    10741 gttgttgatc tctgtttcaa atccttctcc ccaaatgctt tcccctccag tgccatcgcc
    10801 tttaggatcg ccaccttgga tcatgaaatc tttgatgacg cgatggaaag tcagtccgtt
    10861 gtagtaaccg tcttttgcat gtgtcataaa gttttcaacc gcttttggtg cgtgttcggg
    10921 aaataatttg attttgattg tgcctttatt cgtcaccatt tcgaccagat cttcattttc
    10981 ggcgacatct ttcgtcaatt gtggtaattc caatgcgttc aaatcgacgc tactggcagt
    11041 cgtgctttcc gcagtagatg tactttttga actttctgta gacgaactag aagaagtcgt
    11101 gttgtttgca gaacaaccag cagcaaacag caatgctgct gctaaactaa tacttaaaat
    11161 aactttattt ttcttcataa ctgttccttt ccaatttact attttgttat ctttaatgat
    11221 aacaaaatag agggaaaata cctacttaaa ttattggaat ttatcattta ttttaaaaga
    11281 aaaagaaaag gtgtagacca tttactttgt gtttgctata atgtaagtgg atacaaaaac
    11341 gaaaagatgc aggaggaaat ggtaatatgg gaaaattagg aatctcgatt tatccagaac
    11401 ggtcaacatt tgaaaaggac aaagcgtact tggatttagc tcataagtat gggtataaac
    11461 gggtttttac cagtttgctg cagatcaatg atgataaaga aaaagttctt tcagaattta
    11521 aagaagtagt tgattacgcg aatagcctag gcatggaagt aatggttgat atcaatccag
    11581 cgttatttga acagttggag atttcttacg atgacttatc ttttttccat aagatgggtg
    11641 cctacggggt ccgcttagat atcggattca caggagcaga agaagcaaaa atgacgcgca
    11701 atccttttgg tatcaaaatc gagatcaata tgagttcggg cacgaattac gttgacaaca
    11761 tcatgagtta ttcgccaaac acggataatt tattaggttc tcataatttt tatcctcatc
    11821 gatactctgg tttaggatac gaacactttg ttttttgttc agaaaaattc agaaaatata
    11881 atttgaatac gatggcattc gtcaattcgc aatctgctga gtttggccct tggcctacgc
    11941 aagatggctt gtgcacgctg gaagaccacc gagatcttga aattgcgaca caagtgaagc
    12001 atttaatcct tacaggattg attgatgata tttctattgg aaatgcgtac gcaagtgaag
    12061 aagaattgaa agaaatggca gaagcattta acgcggatta tccaacattg aaagtagata
    12121 cggaagaagg tattacagaa aacgaacgaa tctgcttgtt tgataacctt catagttatc
    12181 gaggggatcg ctcagaatat atccttcgtt caacgatgac tcgtgtatac tacaaagata
    12241 aagatttccc gcctcataat acgcgagata tgcatcatgg ggatgtactg attgacaatg
    12301 aagggtacgg tcaatacaaa ggagaaacac aaatcgcctt gaaggatatg aaaaacgacg
    12361 gacgtgtcaa tgttgtagga agaatctccg atgacgaatt attcctcctt gactttttga
    12421 aaccatggtc aagctttaaa ttaattgaaa ataaaaaata gataattagt tattgcagaa
    12481 ataatccgct agaaatagcc agttatttct gcttttttat tttgtttttt tatagg
//
LOCUS       DACCYH010000086        12433 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.090, whole genome shotgun sequence.
ACCESSION   DACCYH010000086 DACCYH010000000
VERSION     DACCYH010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.090"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..191)
                     /locus_tag="IVY85_002279"
     CDS             complement(<1..191)
                     /locus_tag="IVY85_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045281.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGC"
     gene            complement(201..1016)
                     /locus_tag="IVY85_002280"
     CDS             complement(201..1016)
                     /locus_tag="IVY85_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8045282.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1278..1721)
                     /locus_tag="IVY85_002281"
     CDS             complement(1278..1721)
                     /locus_tag="IVY85_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP8045283.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(1855..2193)
                     /locus_tag="IVY85_002282"
     CDS             complement(1855..2193)
                     /locus_tag="IVY85_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8045284.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2181..2558)
                     /locus_tag="IVY85_002283"
     CDS             complement(2181..2558)
                     /locus_tag="IVY85_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8045285.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2782..3975
                     /locus_tag="IVY85_002284"
     CDS             2782..3975
                     /locus_tag="IVY85_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8045286.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4141..4563)
                     /locus_tag="IVY85_002285"
     CDS             complement(4141..4563)
                     /locus_tag="IVY85_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8045287.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            4958..5152
                     /locus_tag="IVY85_002286"
     CDS             4958..5152
                     /locus_tag="IVY85_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045288.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            5149..5595
                     /locus_tag="IVY85_002287"
     CDS             5149..5595
                     /locus_tag="IVY85_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045289.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLS"
     gene            complement(5805..6977)
                     /locus_tag="IVY85_002288"
     CDS             complement(5805..6977)
                     /locus_tag="IVY85_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8045290.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(7183..7998)
                     /locus_tag="IVY85_002289"
     CDS             complement(7183..7998)
                     /locus_tag="IVY85_002289"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8045291.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(8148..8594)
                     /locus_tag="IVY85_002290"
     CDS             complement(8148..8594)
                     /locus_tag="IVY85_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP8045292.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(8591..9595)
                     /locus_tag="IVY85_002291"
     CDS             complement(8591..9595)
                     /locus_tag="IVY85_002291"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8045293.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(9635..9964)
                     /locus_tag="IVY85_002292"
     CDS             complement(9635..9964)
                     /locus_tag="IVY85_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP8045294.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(10090..12417)
                     /locus_tag="IVY85_002293"
     CDS             complement(10090..12417)
                     /locus_tag="IVY85_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8045295.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         3514 a         2430 c         2272 g         4217 t
ORIGIN      
        1 atgcaacctg gaccaataaa aagtaaagaa aaaataaagg aaaagacatt ccttccatct
       61 atcgacaaaa ggaacgcagc tgctatatac aagatattga cacaccaaaa acaaaggcta
      121 cttgccaatg attgatacaa ccaataaaag aattccatat aaacattttt gtcaaaacgc
      181 tgctctttca taatcacctc ctattccttc attcctgatg aagcgatccc ttctacaaaa
      241 tatttttgca tcgctaaaaa tataaaagca acaggaagaa cagaaacaac tgaggcagcc
      301 atgattaatg cgtactctgc atcaaatagc ccaataaaca ttttcaaccc taactggatt
      361 gttttatttt ccgtagaaga caaataaatg aatggcccca tataatcgtt ccatgtatta
      421 acgaatgtga tgatcgtcaa actggcaatc gccggttttg taagaggtaa aatgatcttg
      481 cgatagattc cccactctga taagccatcg atccgcgcac tctcacataa agaatctggg
      541 atactactgt agtattgctt caacaagaac acaccaaaag cattgaacgc ttgtaatagc
      601 accaacgacc acaacgtata tgttaaaccc acttttctca tcataatgaa ctgcgggatc
      661 atataagact gccaagggac agcaatcgtg ccgatatatg ctaaaaataa aatatcgcgc
      721 cctttaaaat ttagtttaga aaacccgtaa gcagcaaaac tagaagtaaa caactgaatc
      781 aacgtgatca caacgcttaa aaaagctgta ttcttaaaaa atgtcactaa aggaatacga
      841 gaccaaataa ccagataatt ctctaaatgc caagttttag ggatccattg aatcggaatc
      901 gtgaacactt cattatttgc tttgaatgaa gcagaaacca tccataaaaa aggaatgatc
      961 gtcaataaag ctaatacaat cagcagaatc atacccaacc attgaataaa gggcatgttt
     1021 tttgaacgaa tttttgcttt ttttctttca tttcgaacac ctccaaagtt ataatcttca
     1081 tttctttgta atgattacaa tccagaacaa cggttattat agctgattca tttccgttct
     1141 tcttatgttt gtaagcgctt atttaacaag ttatgtaaat ctctttattt tttttgattt
     1201 aaaaatgaaa aaatacaaaa gcaagactgt catacgtctc gcttttgtat tgaaatttct
     1261 tcgattagag atacttatta ctgttcaaaa gcttctttga aacggtgaat caattcattg
     1321 actacaggtg ataaaatccg attctttttc cagactagca tgcagttcgt ttcaatcatt
     1381 gggtcaagcg gaacgaatgt cactcgcttt gaaagatgac ttacagcacc ttctatgact
     1441 aaagcagcac ctacctgatt ctcaattagc gcaaatacat tgaaaatcaa attgtacgtt
     1501 cctgtgatca gcaactgctc cttttttatg cctaaccagt cagcaaacat tcgctgtatt
     1561 tctggacgct ttgagatcaa aagaggtaca ttcgcaaagt cttcttttgt tatcacgtct
     1621 ttttttgcta aaacagaatt ttctaaaatg agcaatcccc atttttcctt tcttggtaac
     1681 aaaatcgttt cgtatttctc tgtatctact ggatctagca aaatcgctaa atctaacagg
     1741 cttttttcta gtcactccgt gatatcatca cttgttccgc tgtggatatg aaattgaacg
     1801 ctgggataat cacggatcaa cttttctagt aatagtgcca atgtattcga attatcagct
     1861 tccacacagc cgatcgtaaa tcggccgctg aataattgtt tttttctgtt ctcgaattct
     1921 tgagtggttt gattcgttaa atctaaaatt tcttctgctc ggctttttta gaaaataacc
     1981 tgcttctgtc aagatcattt tgcggttttc gcgtacaaac agttctgttc ctagttcctc
     2041 ttctaactcc ttcagttgtc ggcttaaggt tggttgtgta agatgtagaa cttctgcggc
     2101 ttgagagatt gttccttctt ctgcaattgt ccaaaaatat tttagtaagc gtaactccat
     2161 agcgatgctc cttgattttt ttacttgata cattccattt tatttctaga taacacacga
     2221 tcatatttct tgatttttcc ttgtaaacgt tccaaacact cttgcatttc agcaatttct
     2281 ttcgtcaaaa gttcactttc ttcaatcaaa agcttacgcc gctcaaatac agttgcttct
     2341 ccttcgctag ataggcgaat atattcaatc aaagactcga tcgataatcc ggcatttcgc
     2401 atacagatcg taaagtcgac ccattttagg tcttcttctg tataatcacg gattccgcct
     2461 tttgtccgat taactggtgg aatcaacccg attcgttcat aatagcgcaa tgtatcggcc
     2521 gaaatgccct tttcttcact gacttgtttg atattcatct gatcactctt tctcttaatt
     2581 tcttgccact ttacttatgt attttattta ttctacaact ttaagtgtac tccaagtcaa
     2641 tactcatttc tattaaaact aagctggatt atctatatcg gttgtttgtg tattatttca
     2701 aataaaaaag tcttgttttt ttattctttt ttggatatac ttttaacaat ttagggaaat
     2761 tttaggaaaa ggggtataaa aatgacaacg tatcagcgaa aggtattatt atcgacatcg
     2821 gcgggaattg ctttagagaa tatggatatt atgtttttag ctttttcatt atcttcaatg
     2881 attacttctt ttcatattag tggtacgcaa gcgggtttca tttcaacgat cactaatttg
     2941 gggatgctca ttggaggtat tttttttggc atcatagctg ataaatatgg acgtgtgaaa
     3001 gtattttctc aaacggtcct tctgttttcg attgcttctt tattgatgta ttttgcttca
     3061 aacatctact tggtttatct ctttcgcttt atcgcaggtg taggagcggg aggagaatac
     3121 ggggcgtgta tgtccttgat ttccgaaagt ttttcaaaaa aacaaattgg acgagcttct
     3181 tcggttgctg gtattggtgc gcaggtcggt gcggctttgg ctgcgatttt agctgctgtg
     3241 atcattccaa cgtttggttg gaagatgttg tatgtcgtag gtgtactgcc ggtgttgatg
     3301 gtcttgttca ttcgtcgcgg cttgactgaa ccaaaggaat tcaaatctgt acaatctgcc
     3361 gaaaagaaaa ccaaacttag cagactgttt gaatcaaaac atcttgcttg gcaaacatgt
     3421 ggccttagta tcatggtaac tgtccaaatt gccggctatt ttgggttaat gaattggctg
     3481 ccttcaatta tgcaaaatcg attagggctt tcggtttctg ggtcttcatt gtggatggtc
     3541 agtactattt taggcatgtc tttggggatg atgacttttg gcataatcat ggataaattg
     3601 gggcctaaac tagccttttc tttgttttta atttgttcat ccttatctgt ctatctactt
     3661 gtgttagccc atagccagtg gagtttggta gcagccgctg tgatagtcgg ctactttatc
     3721 aatggcatgt atggtggata tggcgcagtg attagtagtt tgtatccaac agaaatccgc
     3781 gcgacggcga ataatttcat tatgaacctt ggacgagctg ttggtggttt ttcttctatc
     3841 gtcatcggtt tcttgatgga tcattatagt ttaactgccg taatcttgtt tttgagcggt
     3901 atttacctag tcagtctgct ggtcttgaca actcttactg gtgttacagc attaagggaa
     3961 agcttgcagc attgatagtt gagtctgtaa atgacaagaa agaggtgtgg caaaagcttg
     4021 catctgcggt aataagacca gccaaatcaa aaacatgaga cactgtttcc gtttggctgg
     4081 tcgtattact tgatgcaggg cagctttttc aactgctaat ttccttctat ctggcggcga
     4141 ctattctttg acgtccgtca tttcaaagta ctttactaat aacttagcat aacgaaaaac
     4201 tgtggataca ctcgtatatt gagcgtctgc ataatcttcc aaatttttct caggatgttg
     4261 atacatgaat tcaatcaaat gataattata tgattttctg tagaaatgaa gataaataat
     4321 gtagattggg aagttctcct gcttttccat agaaaagcgc cgattattct cattcttcaa
     4381 aatgacctta tcaggatatt tttggaacaa ttcttctaaa tctgttagat aattcaagat
     4441 agacttactt gataccttca attcgttttg tagttcttct attgaacacc aacgttctgt
     4501 ttcaagcagt aaatgaatca ttttaatttt cttttgagaa acttttgtta aaaaagtctg
     4561 catattttct cctcctgtat ctactttact atactacata caagaaaaaa aacggataca
     4621 aatatcatat tgtcacaccg tcaatagtgc ataaacctgt tattatttct ctttttttcc
     4681 taagtattct attgatatga atcatttaat cgtttattat ataaatgtca ataatctaat
     4741 aataaaataa cgtacatatt ttattattgc atacttaata acagaactat agcaaaaaat
     4801 cccgtgtatc aacacgggat tagattaagt ttcctcaatc actattcata acacaattat
     4861 tataacaagt aagtaaacaa ttgaaaagga catttattga aaacggttgt tttttactta
     4921 aggaggaaat ttagcgaaaa aattagttgc attgtttttg gtcgcaggaa gtttgttgag
     4981 tgggtgtggt gctactacgt ctactactca aagcacaagt tcagatcagg cttccagcag
     5041 cagtaccacg atctccgaaa gtacggcaga ttcatcgaaa gaacaagaac aaattgaaaa
     5101 gaaaaaagaa gagaaacaaa aagcggaaaa agctgcacta gaaaaaaagt gacagaagcg
     5161 gaatctgctt tgtcacaagc tgaagcaaat cctacaaaag aaaactatgc aactgcttca
     5221 gcagctatcc aagcgctacc tgaaaacaaa caagagttga caagccgtct tgctgcaatt
     5281 gattccacaa tcaaagcaaa agaagcagaa gaagaacgga aaaagcaaga agaaattgca
     5341 cgacaacaag cagaacaagc gcaacaagaa gcaatcgcac agcaacaagc agaacaggcg
     5401 aggcaagaag caatcgcaca gcaacaagca gaacaggcgc gacagcaatc agcagctgaa
     5461 caggcctctc agcaggcaca agccgcagct cctgagcaaa ataatgaaca aactgtctat
     5521 gtcacaccga ccggttcaaa ataccataca cataaatgtg gaaatggtac gtatacccct
     5581 gccactttaa gctaagctca agctcgtggt ttaacccctt gctccaaatg ttatggaaac
     5641 taattcaaaa gttatactat aataataaaa ttcttctgaa acataaaaaa atagaagatg
     5701 gcgttccaga tagtaaacca aaaataacag tttacacgtc catttcgtca gcttctctct
     5761 tttttcattt agtctgttcc ctctgctttt ttggacatct tcattcattc tttctcaatc
     5821 aaatagtaca cctctttttc ttttcgcagt ttatatcttc cttttttcaa tttcattgaa
     5881 aactgtttcg aatgtttatg ttttacatgg attttaagca ggagctcctc atgttgcaag
     5941 cgatagttcc tgccttttaa atcagaaaat ctcactagtc cgtattcatc agaagctttc
     6001 acaaatggaa ctttttctct agtaatatag tgtttgccgt tgagcgtttt tacagaaaag
     6061 accattcctt cgattggtcg tccgtttgtg ttctttacat aaaaagataa aggatagttt
     6121 cgcttagcga tggccaaaga atctaaataa gcgatgagta gccaaactaa gccgattgat
     6181 ccaagtagta taccgataaa ccaaataaaa aatttccatt ttttccattg atcaattcct
     6241 tggatcgctt tttttgcctc ttttgcttga taaggaatac gatgccccgt cacaagcagt
     6301 cgatgtgtat tgaccatata aggagtacag gtaagcaaag tcacgagatc tctgcctttt
     6361 tggataccca aagaatctac ttcatcaggt aagaccactt ggattttttc tacttgatac
     6421 gccaatgtct ttccattgat ctggatgaaa aactggtctc cttttttcaa cttaggcaaa
     6481 tctgtaaata attttgcttc tgggagtcca cgatgcccag aaagaacggc atgggtgttt
     6541 ttcccgccag tcggataaga actttcttcc aataatgacg ttcctttttg caaaaatatt
     6601 tccgttgtct gatcgaatat aggtaagctg acattgattt ttgggatttc gatgactcca
     6661 agcatatgtt ccataaaaaa ggcattacta ggtaaatctt ttaatgcttt tgcatcaact
     6721 gcttggttga aactgcctat ccctggtgat acgttttgct ctgccaactg ttggtttttc
     6781 tttgtcattt tttcctgctg tttttttatt tcctctgaat tcttattcga tgcttgcttt
     6841 tgataatgga tgatcatttg ctgggcaaga aaatcattca gtgaatcttc tacaaaagga
     6901 taagaaaaga tcccgatccc aacgactaaa atgattgcca tgaagatgtc cagtattctt
     6961 attctgcttt tattcatcat gtccacctat tttctaatat gtatatgaag aggttgtgaa
     7021 atcagcgcct tgacctgagt attagcgaag caaacggcaa aatagttcct cctattttgc
     7081 cgtttgttga gcgaatacca agagaccgac cacttgtcac aacctttctc cccatttgaa
     7141 tcactatcgt ttatttatcg tatgattttt gtttccaata gtttaagctt ctgtttcttg
     7201 atggcgtttt ttgaaataga tcactgctcc tccaactaaa gcagtaccta ccaagatgaa
     7261 tacgatgatc cccataccac cagttgaagg caagattcct ttaggttggt tatcgatcgt
     7321 tgtttcatca gaagttgttg gatcatcatc tactgtaaat tcagtgtacg cagcttcatt
     7381 cttaggtaat gcgtagcctt ctggtgcttt tgtttcttga aggcagtatg ccccttgttt
     7441 cagcccttcg attgaaaatt tcccttctga atcagaaatc aatttcactt cttcccagtc
     7501 agaacctgtg acttcatctg accaagcata tgggtattct cctgaggcaa ggtttgctat
     7561 ttttttcatt aatttaacag ttggatcttt tgctcttaga atagaaaatt ctgcatcggc
     7621 taatttttct tgcgtgttca catcttgttt gatgaagtgg aaatcaccgt agttttggac
     7681 attttctaca gtaatcgtgt tatccgtagt tgtattttcc cgattgacta caccgtttgg
     7741 attcccaaga ttggcttcat ctgcgccatg gatgataccg gttgtttgtt gcaattttcc
     7801 gttttttact ataaaagtag tttctttttg tggattggtt aattgataac catctggtgc
     7861 ttttgtttca actaaagtat aattgccgtc tattaatcca ttgacactga tggatccttc
     7921 agtatcagtt gtgaatgtca acgctgactc agcaggatca tagagagcga caccagtgtt
     7981 ttcatcaaaa ccggtcacga attgattctc tgaatttttg atctggaatt gcgcccctgc
     8041 taacttatct acccctgaaa agttcccagc tttctggatt ttgatattgc tttctttgat
     8101 gacattttta gcatataggt gaatcgtgtc taaaggtttg tcagggtcta gggtaagctt
     8161 aacacgatat ttggcgacgt ttcgacaccg ggctttggtg tttctacaaa gacgtataca
     8221 gcatctttcc catcagattt acctggaaga gtcaggttag ctaatccttc accagaagtc
     8281 gttacacttg cgaagggtgt agcgtctgct ggaactgctg tgtctttact tggatagtac
     8341 gtagcagtga tgtcatatac gttgaattca acgccgttca acggttcgct attcggaaat
     8401 gtcaattccg ttccgttgtt tatttgattt tctggtgctg aagtgaattt caatttatgc
     8461 aaattaaccg taacattttc tggtgtaggt gatacggctg cagaaacctc tgcagttcct
     8521 aggccgagaa cgaaaggaag cagcatgata gcagttccta aaaacgtcca tatttttgtt
     8581 ttgatgtttt tcattttatt tccctcctat tgtttccttt ttgttgtttt ttataaaacc
     8641 aagctgtccc tgttgaaagg actagcaata accctaaaat tgagaaacct ccaagcatct
     8701 cttcgcttgt ttttggaaag cttcctccat ttttttcatt tgatggacca tttgtctgca
     8761 tctgaactgg cgtttttcca ttattcgtcg gatttgcttt tggttcgttg atgatttcca
     8821 atggtatagg atgaagcgga tcataactgc ctttttcaac ttcaaagaga atggttgatt
     8881 gactcaatat atatccttta ggcggtttaa tctccactag ttcgtaactt ccaaaagaca
     8941 atccatggac agaaaatgtt ccatctttac tggaagtaaa aatcgttgct cttgaatcat
     9001 ctttggctac ccaatgattt atcccgtttt cttgcgctaa gtattggtca ttttttttga
     9061 ctagaaattg agcatctgct agtttttgtt tcgtttgccg atccactttg acaaattgtt
     9121 tgcctcctgt ttggatggca agatgtgttt ctactgtttg attgtcagtc gtcaaacgta
     9181 catcgttatc aaatgtcgtt ttttcactgt tttctttcaa tgtcatttgg taacggatcg
     9241 ttaatttttt tccagcaaac gaactaagat cttttccaga atctgataag acaaaaccat
     9301 gttttatttt ttgtgtgtga aatgtatgaa tcttgtctcc accaatcaaa aaatcgatcg
     9361 tttctggttc tagccataac tgaggatcag ctgtatcttt gatcgtatag gttcctatct
     9421 cgttgatgtt ctcaggaatt gttacggtga tgagataagg gactttatcc ccatctgcaa
     9481 aatctgtctg cttattctct actgtttttg ttaatgtgag tgcagcaggt attttttgtt
     9541 cgctctttgg atacaagtgg atggtcgtca atttatgatt agatgaatct gtcacaggaa
     9601 gcacaacgac aaatggtgct gatttttctt ttacttaatc tgaagtcttt atttccgtaa
     9661 atcggtaaac agcgtcacga ccttgctcat ctttgtccga tgcactaaaa gaagcaatgc
     9721 cctcttcctc gtctactgtt tttgtcacat tttctgccag tattttttca ctatcacttt
     9781 tttgtgctag ttttctttgt gcgtcttcca cactacttcc ttctgaccgc agcttgtaaa
     9841 attcatccga gacatcatat atagcgaaag taacgccgtt caaccctttt actccttgaa
     9901 agttatccgg gatatttatt ccgtcgtttt gactgatact tggcatctta tcgctttcga
     9961 acattcgatt atgcaaagta aatgtaatag gtgattctct agctgacaca gtcggagaac
    10021 ctataaaaaa gaccagtagg ataaaaaaga gaacagcaaa tatatccaca cgtacatttt
    10081 ttgttttttt catcgctgtt cacctgcttt tttcttacga tggataaaat aaaagaagcc
    10141 agaaaaaata aagatcagtc ctaaaaagac aaagggcagt agacctatcc cacctgtcga
    10201 aggaagcaag agagtcggat tgatcgtcaa cttagcggta tctgttgcga taatcgtata
    10261 attattgttt acacttcctt cgcctaaact aatcgttact atttgtgatc caatatctgt
    10321 ccggctgtta actgttaggg tataatccaa tgattcgcct aagacaacac cggtaacgtg
    10381 gagatttcca accgaaatcg agtgactttg ggcatcataa ttaaaggcag cgtcatcgac
    10441 agtaatcact gcgttagctt tatagatggt ataagttcct gatgtgttgg ctatatcagt
    10501 aaatgacaac ttgggatttg ctgctttgac tttgtctatt ccgctattcg ttagtttaat
    10561 tgtatagctg ccagcattaa ccatattttt tgctgattgt gcatctgctg tgtaatccat
    10621 actgctaagt gccacttgta ctggagtaag cgaactagtg acaccctctg ctagcttgac
    10681 atggattgac gggacacctg cttttccatc atatgtctta ctgccactga gctctaccgc
    10741 accgctgatt tgcttaatcg tatagacatc agatgtctgc acgatagtaa ataaatagtt
    10801 tgttctggca gctagtttag ctagtccgct ttttgtcaat tgccaagacg tataggttcc
    10861 aatttcagta ggcgcagtta cttcaactcc cttttcattc atcgctttga aatcagttgg
    10921 attccatgtg atttgatcaa tactgagatc attgtttaaa agggatgttg attctgtttt
    10981 atcattaaag gcatatttaa cgatcggtac aacagaaaga ccatcatagt acttactgcc
    11041 atttgcattg attgatactg ttgcatttgt cttataatat aggatgactt cagaaatctg
    11101 ttttccattt gcgtccttta cgattacatt tccatttgta tctaaactag ctaaaacact
    11161 cattgctgat tggttctttg tttctatcgc tgaaacagct gtaaaggtat atccgttgat
    11221 agtaggcgta ttagtcgtca ggtcttgtgc agcacttccg taagtaagat tcattatttt
    11281 agaaggagca atcatctgtc caaaatagtc ttgatactta atagtgattg gtgtttgttt
    11341 gcctgtatag gtcaccgtat cgacgttact ttctagggct gtgaaagaac cgtttgtttg
    11401 tgcaaaagaa tagcccgtta ctgctgttgg agtgataacc actttgtcac ccacatggat
    11461 tccagataat gtagtcccgt acgttccgat ttttgctgct ccaccattga ctgtataaga
    11521 aaaagttcct gtccgattcg cattgacctg aaccgttgct gcttgatagg ttggaaaatt
    11581 tgaaacattt cccttaccat caacgatctg cccatttttg atcgagtacc ccgtgaatgg
    11641 aatatcattt gctaactgta taccgattga aaaagtactc atacctcctc cggcaggaag
    11701 ccctccccat gttgagggtg taaaagaata acttttatcc gcattgggtg taactgaatc
    11761 gatcgtattt ccagcggtag ccacccccat atagatactt tttgggaatg tgatagtagt
    11821 accatttact gtggagccaa taaacagatt atggatatct ggacggttat caataaaagc
    11881 gattgctttg gttacgcctt gtaacgcagt tttatccgca ctcatcgatc cgttaaccgc
    11941 aacaatgact ccatctttgg catatatcac tgtatatcca tctggtatgg tatgtccgtt
    12001 gaaattcgca gtggccgcaa cagtactaaa tgtctggctt ctacctgttt ctgaataagc
    12061 tttctcccct gctgcttttg ctgctgtgat ttctttttct gtcggatcaa tcagttgagt
    12121 cgaggatgtg gttgaggttg tctcgttgtt gctagactga ttggtgcttt ctatggactg
    12181 tatttcagaa tagggtggac tatttgtctc ctctgtcgtt tggttagcaa aatttgacgt
    12241 ttctgtataa acgtttacat aaatacttga taagctccct aagaaagtaa aaatcaagat
    12301 gatcgaaatc ataatgggat gtaagtattt tcttttagat acagctttga gtctagaaac
    12361 aaaatgtttt tgacatatac gttggatttt attttgagtt ttcttaatca ttttcatccc
    12421 tcctctcaat aaa
//
LOCUS       DACCYH010000087        12295 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.091, whole genome shotgun sequence.
ACCESSION   DACCYH010000087 DACCYH010000000
VERSION     DACCYH010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.091"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..1210)
                     /locus_tag="IVY85_002294"
     CDS             complement(<1..1210)
                     /locus_tag="IVY85_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8045296.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVL"
     gene            1379..2338
                     /locus_tag="IVY85_002295"
     CDS             1379..2338
                     /locus_tag="IVY85_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8045297.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            2603..3082
                     /locus_tag="IVY85_002296"
     CDS             2603..3082
                     /locus_tag="IVY85_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045298.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            3115..5343
                     /locus_tag="IVY85_002297"
     CDS             3115..5343
                     /locus_tag="IVY85_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8045299.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            5472..7115
                     /locus_tag="IVY85_002298"
     CDS             5472..7115
                     /locus_tag="IVY85_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8045300.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(7188..7733)
                     /locus_tag="IVY85_002299"
     CDS             complement(7188..7733)
                     /locus_tag="IVY85_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP8045301.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(7756..8241)
                     /locus_tag="IVY85_002300"
     CDS             complement(7756..8241)
                     /locus_tag="IVY85_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8045302.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(8231..9043)
                     /locus_tag="IVY85_002301"
     CDS             complement(8231..9043)
                     /locus_tag="IVY85_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP8045303.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(9182..10714)
                     /locus_tag="IVY85_002302"
     CDS             complement(9182..10714)
                     /locus_tag="IVY85_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP8045304.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(10823..11854)
                     /locus_tag="IVY85_002303"
     CDS             complement(10823..11854)
                     /locus_tag="IVY85_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8045305.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         4066 a         2271 c         2362 g         3596 t
ORIGIN      
        1 ttaatacttt gatgtggttg tttaagcatt ccaatggtcc gtttgaatag ctgtagtgaa
       61 gcgcattttg gatctgtggc agaaactttt taaacgttcg taaagtggtt gtataggttt
      121 ctggcagacc gctaatgtct ctagttaaac aatcctcaaa tagtgactga tctcttttct
      181 ttaccgcata aagaatgtct tggtataact gatagccttg gcgtaagttg ggacaagcag
      241 ctaacagtcg atcggcaatc tcactttcag tcaaataatc tttaaaactg gcacgccaaa
      301 cccgtttttc atagtttaac tttgcttctt ctttttgtag gagtttccaa tagcgcttca
      361 attttttccc taaccctcgt tgagagtggg aatttccttt aagtcgtacc gtggtttctt
      421 tcactcgatg attcaagaaa gttctgccaa tatgttggac gatgtggaaa cgatcgatga
      481 tcaactgagc attcgggaaa agctttttca ccaacgaaac ataaggcgca tacatatcaa
      541 taacaatcaa ttgaacggct tcacgagtcg ctaatgaaaa ccgagaaaaa tagcgttcta
      601 aataaggcaa gcgtcgattt tcgacaatat ccaataatgc atggctttgt ccgtccatca
      661 tgataaaact cattgcactg gcaacacttc gaaccgattt gaattcatca aagcataaga
      721 cctttggcaa agtctgtttg aaaggattca atgggcggta gaatcgcttc atgacacgat
      781 agaccgaaga agtggaaata tttttctgac gagcgatttc cgtcatggaa gtattctctt
      841 ttaagcgagc aatgacagac caacgaactt tttgactaat gaaacagtgt ttttcagcaa
      901 cagaggtatc cgctacgaat gttctttggc aagacttaca tttaaatcgt tgtttcttca
      961 gctccagata agtgcgatac tcagacacat cattcagtaa caaccgaacg gttgtcgtcc
     1021 cccaacgaat gatcctagag gggacgacac aaagacaatg ggaacactct tctggcttgt
     1081 acagaagagt tcctttgtaa atcaagcaaa tttgcccttt gtatttttct ttagttaaac
     1141 aattttcctc gaaatagata tttaggtcta gaatatttag ggtatctttg ataagctgag
     1201 tatgagacat cttgaatcct cctatgaatt ggtagccaaa ttaattctaa aggatttcag
     1261 ggtgtctttt ctatattttt ttatacgccg attgtaaaat taagctagac aactaaaaaa
     1321 gtcatttgtg ctacactcaa aatagttccg accaaagaat tataaggagt gagtacaaat
     1381 gacctatacc catcttacaa cggatgaact tgtaataata gagtcttatt tcaaaatgaa
     1441 tcaatctgtt gctaaaactg cccattgctt gaatcgttca agacaaacga tccataaagt
     1501 atatctgttc ttcaagcaag gaaaatcagc cttagaatat tatcaacagt ataagaaaaa
     1561 caaatcaaac tgtggtagac gtccgcttgt tttacccgag gaacaatcag aatatattca
     1621 aagaaaggtt gttcaaggat ggacacccga tgtgattgtt ggtcgtgcag cgtttcctat
     1681 tagttgttct gctcgtacca tttatcgtat gttcaaaaag gggctatttg attcttctga
     1741 cttaccgatg aaaggcaagc gtaaaccgaa tggacatcaa gaaagacgtg gaaaacaaac
     1801 tttccgccgc tctattcatg aacgtgaaaa ggattatagc caattctcaa atgagtttgg
     1861 tcaccttgaa ggtgacacta tcgtaggtct gaaacataaa agtgctgtaa ttaccttagt
     1921 tgaacgatta tcaaaagtta tcatcacatt gaaaccgtgt ggtagacaag cgattgatat
     1981 tgaaaaaaaa ttaaatcaat ggtttgaatc tgtaccgaaa aacctattca aatccatcac
     2041 ttttgattgt ggaaaggaat tttcaaattg gaaacagatc agtaatgtca atgatattgc
     2101 catttatttc gctgatccag gaacgccgtc tcaaagaggc ctaaacgaga attctaacgg
     2161 attgttacgt agagatggtt tattgaaatc tatggatttc aattcagtag atgaattttt
     2221 tattcaatct gtcgcatcta aacgaaataa tattcctaga aaatcactgg attatcgaac
     2281 acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaact taatttgaca
     2341 attaagaata caaaaaagat gttggtgaag ttcaccaaca tcaaaaagta tagagccaaa
     2401 atattccaac atcatcttgg actttctaca tgttactaat gagcgtgtaa aagaacgaga
     2461 tggtgttttt atttctatca atgaagctct ttttctttgc aatcctcact tcaatcctct
     2521 ataatgtcgc atatagttta gtaaatgagt aaactactaa tttacttatt tactaaaaat
     2581 aaaaggatga ggagttaaaa taatggaaat accaacaaat ttaaaacata aaccagtaat
     2641 cgttgtagaa gactatgata aagttgatgg aagaaatgca ttaaatatgg atgcaaaagg
     2701 gctttcactg ggccttgctc agtggaatga tcgtggaaaa gtcgatatct caggcaaggt
     2761 atggcgacat actggagaaa aatggtcacg acagtcggaa gaattaccaa tcacacgtat
     2821 tttagattta gcgatcttag ctgcgcaagg aagtttatac ttccaagatg cttatcgtta
     2881 tgaaaaattt tatgatccag aaaatccagt cgtagacatc attggattac aagggaaccg
     2941 aatgacggta gaagtagata ccaaaaatcc aaagatcgat gaagacatca ttttgtatta
     3001 cgatacacta caaaaagacg gagaactgat tggggaacga ttccgtattt taaaacgtct
     3061 tttagatgag ctaggatatt aatagacaga gatgatcaaa aggggaagag aagcatggag
     3121 aatttctcac taacagaaaa ccgaaccttt tcacggctag aaaagcaaat gatctggaag
     3181 aaaccgaaaa gccatcggac aagtgcagaa gaatggcgga tcgcctccga aatcaaaggg
     3241 aattggcaag atcctgaaat gaaaatcagt aacgtacttt tggaaggaga tgccgggtcg
     3301 gggaagacac aattagccaa agcactatct gctgatctgc aacttcccta tacaaaagtg
     3361 acctgttttg cagatatgga caaatcagat gttttcggag ccttattacc tgtagtagat
     3421 tctaataagg aacaagacca agaattacta gaagctattt accaaacaga tacacttgaa
     3481 gctgtactga cactgatcca gaaccattac ggcattgatc ctttttctgc taaagaaaaa
     3541 ttagcagagc tagttcaaag gatcgaagct gattatccag atgcaatcca ttacagctat
     3601 tacccttctg aaatcgttcg agcgattgaa aaaggctatt tattagagat tcaagagccg
     3661 actgtcatcc gagatgcctc tgttcttgtg gcacttaact cagcgctcga accaaatggg
     3721 atgttgaatt taccgacagg aatcattcgt cgccatccag attgcgtcat tgtgatcact
     3781 acaaatcgaa attaccaagg aaatcgccca ttaaatgaat ctttaagaga tcggatgcaa
     3841 catgcggaaa aaatggatct tcctgcctta gaagtcatgg cagaacgtgc gatggcgaaa
     3901 actatgatcc aaaaacaaga acttctctta aagatggcag agattatccg tttgttggat
     3961 gaaactgcaa aagctaacgc catcaaaggt gtagcaggaa tgcgttctta tttttattgg
     4021 gcaaatacct ataagcaagg acaagattta ctccagtcga tttatccaaa agttttgtat
     4081 aaattatcca ctgattcaga tgaattggca attcttgaac aagctttgtc tgacagtgga
     4141 ttgctactgg aacttcagga catattgaga aaagaaaaat ggggtcatcc tcaatctgag
     4201 catacaaaag aaagaaccat tagcgccgaa gaagcaaatg aacgaaacat cactgaaaat
     4261 gctgaagaaa ttctgcgctt agatgaaaag aaatcgttgt ctgctgaaga accaaatgag
     4321 ggagaagaga aacaagaaga aacaatagaa aaaaacgaga ttcctcctca aatgcaaaaa
     4381 gaacgcagta gcgatatcga aggaaaaaat caaaaagaac aacaaacagg agatgattct
     4441 caagaaatgt ctgcttctga catggaagca catgataagg aaatcaagaa gcaactgaat
     4501 aaagaagcgc gacaaatcat gaaggaaacg attcatgaaa aagaaggtct catcgttcac
     4561 cgaccagctt tccaaggaaa aacgccggaa gcacgtgagc tacacatgca agtaatgcca
     4621 atagtagaat cacttagccg gcaaatttta gagttactgg ataatgaaca aacagagacg
     4681 taccaaaaag gaaaatacga aggtcagcgg ttcaatgcaa gcaaagtagc atatggtgat
     4741 ttgagaaatt ttgacaaaaa gaacccgcca catgagcaac cttctttagc ggtagctgta
     4801 cggatcgatg aatctgggtc aatgatacgg gatgatcgga tcgaagcggc aaaaaaagca
     4861 gcgattgcca ttgccgaatt ttctaagaaa gtcgatatcc cattgttgat ttacggagat
     4921 acagcagacc gttcgtcaag agaaaagact tctttgtttt cttataaaga atttgaagac
     4981 ggatttcaat ggttaaatga aaaattagtc actatgaaac caaggcaaaa caatcgtgat
     5041 ggtgctgcac ttcgtttagc agcagaaaaa ttaaacagac aatccgcaac aaccaaactg
     5101 ttgcttaata ttagtgacgg tcagccaaaa gcgttgccgg attatacagg caaaaaagca
     5161 aaagaagata tccagtctgt tttgaaagaa tatgaacgtc aaggaatcct atttgtctcc
     5221 gcagctattg gccaagacaa agaagaaatc aagtcgatct acggtgaaag tcgttttgta
     5281 gatattactg acttgaatga atttcccaag caaatgattc aactgattgc ccgctatcta
     5341 tgataaaata aagctcaacg aacgcactta gcagattcgt tgagcttttc gtttctagtt
     5401 accggtaaca aggatacttc taaaatagaa tcggttataa tcaagaggga ccgttaaagg
     5461 agtgaaacga tatgatacaa gaacaaaaat ggtggaaaga agcagtaggc tatcaaatct
     5521 atcctgctag tttcaaagat agcaatggcg atggtcaagg agacatcaat gggatccgtg
     5581 aaaaacttaa ttatttgaaa gatcttggta tcggctttat ctggatcaat ccgatctatc
     5641 aatctccatt tgtcgataat ggttatgacg taagtgacta tcaagatata gaagagagat
     5701 tcggaacgat ggaagatttt gatttattgc taaaagaagc acatgaatta gggatcaaag
     5761 tgattatgga tcttgtcatc aaccatagtt cggatcagca tcaatggttt gaagaatcga
     5821 aaaaatctaa agaaagcccg tatcgcgatt attacatttg ggtcgacggg gtagatggca
     5881 aagaaccgaa taattggaca tcgattttcg gcggttctgc ttgggagtat tctcatgaaa
     5941 ctggacagta ttatctgcat gtttttgcaa aagaacaacc agacttaaat tgggaaagcg
     6001 agaaattgaa agaagaacta ttcaatatga tccgatggtg gttggataaa gggatagatg
     6061 gatttcgttt agatgcgatt tctcatgtaa aaaaagatga atattcagta aaagccacag
     6121 agaacccttt ctcacccttt caaaatgtct caggaatcga agaacatttg accgatttga
     6181 aacatgtttt tgaagagtac gatatcatga ctgtcggaga agcaagtggt gtcactgcag
     6241 aagaaggacc acaatgggta gggaaagacg gttatttcga tatgatattc gaatttgatc
     6301 atatccatat ctggcagcaa gaaaaagaag ggcaattaga tgtccttaaa ttgaaacatg
     6361 cattaagcgc ttggcaaact tcattggatg gtatcggatg gaacgcttta tatatggaaa
     6421 accatgatgt tcctcgtgcc gtttcagtgt ttggagatac tcgtcccgat ttttgggcta
     6481 tgtctgcaaa agcaatcgct atgatgtatt tctttttgca aggtacgcca tttatttatc
     6541 aaggacagga aatcggaatg acgaatatgc cctttgaatc gatcgatcaa gttgatgcag
     6601 tagattcgaa aagactttat aaaaggctgc ttgcggaagg taagacaaga gaagaagcat
     6661 tggatatcat tcgtgaaaca acgagagaca acagcaggac acccatgcaa tggacttcgg
     6721 aacaatatgc tggtttttct acacatgaac cttggctgat cactaatcca aataccaaaa
     6781 cgatcaatgt agaacaacaa gaatatgaac cagagtcggt cctgcaattc tataagaaca
     6841 tgatccgcat tagacaaacc aataaaggtc tgatatacgg ttcttataaa gaatatcttc
     6901 atgaacatcc acaactttat gtctatgaaa gatatttaga agatgaacaa tatcttatta
     6961 tggtaaactt aacagaatca cttgctgact atgaacttcc aaaagaagca gatcagtcct
     7021 ggacattact cttgtcaaat tcctctagcg gagaatttga agctaaaggc atactagcac
     7081 cttatgaagc tcggttatat aaaacaaaca agtaaaactt cctcgttaaa gttcattagt
     7141 tggactttgc gaggaagttt ttcagattgc tctgtcaatt actcatttca ataattcgct
     7201 ctaggaccac cgatatattt aggacgagag cgaagggctg tcacgtgagc ttgtccaagg
     7261 gttttcaaac gaggtctgac aggtacttgg acatgcttga tatgcatacc gattgatgtg
     7321 tcaccgatat cgataccggc ttgtccaact acgtgttcca cttcgacagg atcattgaac
     7381 tggtcaaaag cagcaacaga acaagcacct ccagcatgta gtgcaggaac aacagagaca
     7441 atttcgaaat tttttgcttc tgccactgtt cgttctacta ctaatgctcg gttcaaatgt
     7501 tcacaacctt ggacagctag cgcaatttct ttaggatcca acaactcttt taacgtacgg
     7561 atgatccatt gaccaacttc cgcactggaa ttttttccaa tatgtcctcc aacgacttcg
     7621 cttgtgctgc atcctaaaac aaaaagatca ccttttttca agtgtgcctc agccaatatg
     7681 tcattaacta tttcagtcaa ttggtctttt aattcttttt ctgacaactt cattttctaa
     7741 aaccttcttt ttcaattaat attgcttccg tgcttgaata cgttgaagac ttgcagttat
     7801 aggaaaggca agtcccattc caatcagatt ttggaagatg tttcctggta tagaagccac
     7861 tccagccggc caatcatata aaatccaacc ggcgatacca tagccgatga tcataactaa
     7921 gcttcctaat aaaagcccta atccccacat acgagtagaa gatttgctga aagaaccagc
     7981 aacatatcct tgtaacccgt ggatcactaa tgaaaaaact gcccactgcg gatatcctga
     8041 aatcaaatcg atcaatagac cactggaagc accaacaact aatccacccg ttggaccata
     8101 aagaaaagca gcggtataga tcccggcctc gcataatgtc acaaatccat tcgtagccgg
     8161 tacagggatc aaaattgtta atgaaagggc aactgtcaac gcaatcagca tagcatagat
     8221 cgttaattgc ttagttttca tttctcattc ctcctttttg aataccgcca gaccagacac
     8281 ttccggtatg atcgttcatc agcacgccat tagaaataca atgggcaaca aattttttac
     8341 tttctgctac tgctttggta aaatctgccc ctaacacaag attcgctgtt attgcagaag
     8401 aaaaggcaca accggccccg tttactgttg tagtgtcgat ttttttatga gaaaatacct
     8461 cataatgatc aattgaatag agaagatcca cagcttcatc tccagccatc cttgtgccgc
     8521 ctttgatcac aatgtttttt gttcctgaag cagtgagttc tttagcgaga tggatcattt
     8581 ctgttaacga agagggaaga gcttgaccag aaagtaaagc agcttctgcc aaattaggcg
     8641 taatcatcgt aacaagtgga aaaagttctt cgatgagctg cgaaatatac aattgattga
     8701 tcacagcttc agtttctttg aatgccaaaa cagggtctaa aatgattggg atattttgtt
     8761 ttgtttttat gaagtctcga acaaccatga ttgcttcact tgaattcaat aacccaattt
     8821 tgataccatc taatgaagtc attttatcaa tcgaaactaa ctgtcgttta agcaattcca
     8881 aagatataga ttccagcata aatcgctcat taatatctag tgcccctata gaagtcaagg
     8941 cgcttagtcc gaatagacca aaattctcaa aagtttttaa atctgtttgg attcctccac
     9001 cagcaaatgg atcagaaccg ccaattgtca agattctttt cataacattt tcctccatga
     9061 ataagagtat aacgaaattt tcaaaaaaga aaaacagcca attggcatat ttttatctca
     9121 tccaatttaa aaaaacaaaa agaaaagaaa gaccaaaatg gatctttctt tccttaaata
     9181 gttaggactt ttgtgatttt aattctttct cgatttgaag ccgttgcact ttcattgtag
     9241 ctgttcgcgg gattgcatca aaatcacgga tgatagggac gttcaagcga ggaagttcat
     9301 ggacttgctc ccaccatcga ttccaatcca tttctttatc aggagctaaa gcaataatcg
     9361 gttgtggaga atgattttta tcccttacaa tcactacttc tgccaaaaaa tctaacgaat
     9421 ctaataaaaa gtcttcaata gctaatgtac tattgatcgt ttcgatcaaa tccacttgcc
     9481 gatctttcaa aaataggtgg ccatgcttat ccataaaacc gtaatctccg ctatcccacc
     9541 aatcgccata aacattttcc tggaaccgtg catcttcctt gtaataagtc aaagcccgtc
     9601 ctttagacag taattgtata tgaccatctg tgttttcagg taggacattc ccaactgaat
     9661 ctgtaatccg agcgcttgtc atgtcttcca gtccaacgcc catatctctt gcatcacttg
     9721 ttttcaaact ctctagtgta tgagccttaa gaatcattgg tccgcattca ctttggccat
     9781 atacttgaag aaataccgca tcctgtgcca tcgacgttcg caaaaatgtc ttcattgtct
     9841 ggttgtttat agcatcaaaa gtggagtgat agtatcggat acccgaaaaa gcttctggat
     9901 gctccttagc agtaaaacgc cactgaacaa agttattcgg atgggtctct agtgcaattg
     9961 gccggtgagc ttccaacata tgaacaactt ttgaactttg tgcgtttgct aaaggcatca
    10021 taggaaatcc catggacatc aaagaagaga taccaatatt gaatctggaa tgcactggag
    10081 agatgtgaaa agcgattaat ttcttttccg aaatttttgt gaaaactgtt ttttgccatt
    10141 tcgtccgcca tcccatagaa tgagcagaat gacaaataag tttagggata ccagttgtac
    10201 cagaagtatg ggtcatataa gaaatttcat ttttgtctaa aaatgattga gaaacagaca
    10261 aagcaggttg ttccattaaa taaagaatag gaatctgttt tgattttgtt ccatttgaaa
    10321 tttctctcac tcgctttttt gtctcatcat caaataaaat gaaagggtct tctaaacgat
    10381 cgataaatac ttccattgtt gttgttggta gatgataaga agtcatgaca ggcactgcac
    10441 caaggtaaga cacagaaaca gctagaaggt atgtgtcaaa agcggaactc ttataaataa
    10501 taactttgtc tccggatttc actccaagat gagccaattg ataagcccgt ttcaacacac
    10561 tttcgcagca ttttttgtaa gttgtttcaa gtcctaattc tgggaaagct gaaagtggct
    10621 catcaaagat aatcggggtc tcggaaaagt tagctgcagc ttcttgaaaa ttcgtaaata
    10681 agttcaaagg ggaatacgtt atggtctcca tcattcacac tccattatct tttgttaagt
    10741 aatatacaaa ttcaagataa tcgtaacata atcctttata taataacata gtttattgtg
    10801 aaaaaaagat catacatttg ttttatatgg aagaaacccg aacgaatgcc gctaattcgt
    10861 gataagaaaa atcaaccatc ttttcccaga tatcttgaaa agaatgggat gagaggtatc
    10921 gattaccaca taaataattt accgcgtcag catcatctaa atatagttct gcataataac
    10981 gatatgtttt tctgcatagt tctggcgata cgtttttata tactgtaatt ttgctcaaaa
    11041 tttggcgaat agtccgagga gaaatttgct gatcatgtga ggaacgaaaa acaaaagaat
    11101 tttcgtccgt attttgtgct gttaacttaa aatagttctc cagaaatggc atcgcttctt
    11161 ttggtattgg tactttattc agtctgtttt cttttctatt cagattaaga atctgcttgg
    11221 tcaaagatac atctttacgt ttaagttcta ctacttcatg gatctttaat ccgcaataca
    11281 gaagaatggc agtaattgca aggttcctcg gtctattctt ttcttcaaag ctagcttctc
    11341 tgcttgtttg aaagccttca atcgggataa acaaatgctc aaaatctttg aaaagttctg
    11401 catcgatata tcgcggagct gctttggttg tttccggtaa acttgtaccc aaaagagaaa
    11461 tctcttcttc aatcagtcct ttttgataca aaaatcgtaa aaaatgattt aatgaatatt
    11521 gcttccttga aatcccgtaa tagctatttt caaacaattg ctccgtttct tggttcgctg
    11581 tagtttggaa cgaacgagta tgtcgataga ttttagctaa gaaattctga taatcttgtt
    11641 tttctaaatg gataatcagc tgatcatcca cttcttcaga aaatactttt ctcaaataat
    11701 cagtaaacag atgaaaatct tttgcgtatt catagcgact gcctaatgaa agggaagaac
    11761 gattttcttc taaaaactgt tgaaagacgg aagggagcaa gtgtgtacgt tgctttaact
    11821 tttcttgcag ttgttttcta gttttgctgt ccattacaaa ttccctccaa tagtgttcat
    11881 tttctatatt ttaccgcatt tcatcaaatg aagatagaca gtttgtcttg atcttaggaa
    11941 gcaaattttt aactaagtac tagctaaaag atatgaagta agaagataca tttttagtta
    12001 ttttattagt gcagtcattc ttttgatctt ttaattgatt tatcctcttt ttagctatgt
    12061 ggttacttca tgagcaatga aatggatttg gttcgttcta atggcaggag gctctttctt
    12121 ttatttccga aaaattagaa aacatttttt cagaatcgat tggctctcca tttattcaat
    12181 aatggtttgt ctaacgatat tgttgggata attcttaaag aagtattaga aatagttggt
    12241 acttcattac tttacatgaa gatttattct atggtaggag caattatatt gagaa
//
LOCUS       DACCYH010000088        12102 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.092, whole genome shotgun sequence.
ACCESSION   DACCYH010000088 DACCYH010000000
VERSION     DACCYH010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.092"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(38..595)
                     /gene="frr"
                     /locus_tag="IVY85_002304"
     CDS             complement(38..595)
                     /gene="frr"
                     /locus_tag="IVY85_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP8045306.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(598..1320)
                     /locus_tag="IVY85_002305"
     CDS             complement(598..1320)
                     /locus_tag="IVY85_002305"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP8045307.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1456..2337)
                     /locus_tag="IVY85_002306"
     CDS             complement(1456..2337)
                     /locus_tag="IVY85_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP8045308.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2436..3218)
                     /gene="rpsB"
                     /locus_tag="IVY85_002307"
     CDS             complement(2436..3218)
                     /gene="rpsB"
                     /locus_tag="IVY85_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP8045309.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3577..4056
                     /locus_tag="IVY85_002308"
     CDS             3577..4056
                     /locus_tag="IVY85_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP8045310.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4273..5364
                     /gene="phnW"
                     /locus_tag="IVY85_002309"
     CDS             4273..5364
                     /gene="phnW"
                     /locus_tag="IVY85_002309"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP8045311.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5357..5485
                     /locus_tag="IVY85_002310"
     CDS             5357..5485
                     /locus_tag="IVY85_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP8045312.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5489..6034
                     /locus_tag="IVY85_002311"
     CDS             5489..6034
                     /locus_tag="IVY85_002311"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP8045313.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6488..8179)
                     /locus_tag="IVY85_002312"
     CDS             complement(6488..8179)
                     /locus_tag="IVY85_002312"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP8045314.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8599..9546)
                     /gene="arcC"
                     /locus_tag="IVY85_002313"
     CDS             complement(8599..9546)
                     /gene="arcC"
                     /locus_tag="IVY85_002313"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP8045315.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9661..10680)
                     /gene="argF"
                     /locus_tag="IVY85_002314"
     CDS             complement(9661..10680)
                     /gene="argF"
                     /locus_tag="IVY85_002314"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP8045316.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10771..12000)
                     /gene="arcA"
                     /locus_tag="IVY85_002315"
     CDS             complement(10771..12000)
                     /gene="arcA"
                     /locus_tag="IVY85_002315"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP8045317.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3299 a         2522 c         2195 g         4086 t
ORIGIN      
        1 aagcggaatg atccgcccct ctgtttttat gattttatta aacttccaat aattcttttt
       61 ctttatcagc agcgatctta tctacttctt tgatgctgtt atctgtcaat gtttgtacgt
      121 ctttttctaa tccgcgcaaa tcatcttcgg taatatcacc atttttttgt tgttttttat
      181 attcatcgat cgcatcacga cggatgttac gaatcgcgat ttttgcattt tctgcttctt
      241 ttttaacatc tttagctaat tcttttctgc gttcttctgt cagttgcggg ataactaagc
      301 gaatgacatt tccatcattg gttggactga ttccgatatc acttgccata atagcttttt
      361 caacatcttg aagaatgctc ttgtcaaaag gtgtgatcat taaaacacgt gcttctggaa
      421 tttggattga tgccatttgg ttcaatggtg ttggcgcacc gtagtagttc acagtaatac
      481 ggtccaaaag gctagcgttc gcacggcctg cgcggatttg cccaagttca cgttgaagat
      541 tttgtgctgc ttttttcatt ttttcttttg cttctgtcat aattgcatct gccattttta
      601 tttccccctt acagttgttc cgatattttc gcctaagatt gcacggcgaa tatttccgtg
      661 ttcatttaaa ttgaagacaa gcagtggaat atcattatcc atgcttagtg aacttgcagt
      721 tgaatccatg acttgtaatc ctttggcaat aacttccaga tgagtcagtt cttcaaattt
      781 tactgcattt gcatctactt tcggatcagc agaataaaca ccgtctacat tgtttttagc
      841 catcaggata acatctgcgc cgatttctgc cgcgcgtaat gctgctgttg tgtctgtcga
      901 gaagtacggg ttgccggtac cgcctgcaaa gataacgatc cgtccttttt ctaaatgacg
      961 ttctgcttta cggcgaatat atggctctgc gatttgacgc atttcaatcg aagtttgcac
     1021 gcgtgtcggt acacccacgt tttccaacgt atcctgtaaa gctaaggcat tcataactgt
     1081 agctaacata cccatgtaat cagcttgagc acgttccatg cccatttgtg ctccgatttg
     1141 tccgcgccag atatttccgc cgcccacaac gattgccatt tcaacgccca gctcgtgaac
     1201 ttctttgatt tcttggatga tttctttgat tactggtggt ttgattccaa atcctgcgtc
     1261 cccagctaaa gcttctccac tcaattttaa gactacacgc tgatatttcg gtgtgaccat
     1321 gttcattcct ccaatatttt ctattgccat tttaccatac ttagatgatt tcgacactct
     1381 aagactggtt tttcttgttt tgcttaaaaa aagggagcgc acaaaccgtg cgttcccgtc
     1441 tgttattcaa cagaattatt ttttcacttg gctcataact tcttctacaa agttatcttc
     1501 gcgtttttcg ataccttcgc caacttcgaa acgtacgaat gttttaacag ttgcgccttt
     1561 tgatgcaacg tatttttcaa cagtcatatc aggatcttta acgaatggtt ggtccaccaa
     1621 agcgatttct gctttgaatt tcttcaagcg tccttctacc attttttcaa cgatattcgc
     1681 tggtttgcct tcgttcaatg cttgttctgt aagaacagtt ttttcatgtt ctaattcagc
     1741 ttctggaatt tgtgattcgt ttacataacg aggattgata gctgctacgt gcatagctac
     1801 gtctcttgca actgtttcgt cagttgttcc atctagaaca gtcaatacgg caatacgtcc
     1861 gcccatatgc aagtatccac caaatgcagc attgtcgtct ttttctacaa cttcaaagcg
     1921 acggaagctg attttttctc cgataacttg agttgcttcg atcaagtcag attcaattgt
     1981 acctttatct gttttgattt tcattgcagc ttccatgtct gctggtttgt tttcagcaac
     2041 aagttctgca atttctttta ctaaatcttg gaacatttcg tttttagaaa caaagtctgt
     2101 ttctgagttt acttcaacga ttgctgcagt gttacctttc acagcaactg aagccaatcc
     2161 ttcagctgca atgcggtcat ttttcttcgc agcctttgcc attccttttt cacgtaaaag
     2221 atcaactgct ttttccatgt cgccttctac ttcaactaat gcttttttcg catccatcat
     2281 tcctacgcca gtcatttcgc gtagttcttt taccatttta gctgtaacgt ctgccatgtt
     2341 taactcctcc tgtgtgtttt tctttttaaa aaaagctgct tcaaaggaga gtgaggcttg
     2401 tcgcccagcc tttgaaacag ctccatcatt caaaattatt ctgctgaaga attgtcgcct
     2461 tcaacaacat ctacgatttc ttcgattgat gttgctgtgt tttcttctgt gaaatcttct
     2521 tcaactactt gatcttcacc ttggtttcct tcgatgaaag catcagccat ttttgaagta
     2581 atcaatttaa cggcacgaat tgcatcgtcg tttgaaggga ttactacgtc gatttcatct
     2641 ggatcgcagt ttgtatcaac catagctacg attgggatat ttaatttgtg tgcttcttga
     2701 acagcaatac gttctttacg tgggtcaacg atgtacatta catctgggat tcttggcata
     2761 tcagcgatac cgcctaagaa tttttcaaga cgttcacgtt ctttgttcaa accagcaact
     2821 tcttttttag gtagaacttc aaaagttcca tcttcttcca ttgcattgat ttgtttcaaa
     2881 cggctgatac gtttttggat agtatcccag tttgtcaatg ttccgcctaa ccaacgatgg
     2941 ttaacgaagt attgtcctgc acgagttgct tcttctttga ttgcttcttg tgcttgtttt
     3001 tttgtaccta cgaataatgc tacgccgcct tcttcagcaa cgctcttcat gtaatcataa
     3061 gctgcatcta ctaatttaac tgttttttgc aagtcaatga tgtagattcc gtttctttct
     3121 gtgaagatat atttcttcat ttttggattc cagcgacgag tttggtgacc aaagtgtacg
     3181 ccggcttcta gtaattgttt catagaaatt actgccatgt ttgtttcctc cgatttggtt
     3241 ttatttttcc tcttcttatc ttctaccttg ccggcaaact ttttcaagca cctaccgaca
     3301 atccaataag aatgtggatt tgctgatgca taaagcaccc tcagctatta tacagacaaa
     3361 agatccgtat cgcaagagca aatatacttt tctgctcaac attcaatagt ttacctgaaa
     3421 gataaaaaga attcaagaaa aacagtagat ttctctttgt ttctttatta tcctttttcc
     3481 ataaaataat tttaggtata aaagacgaat attattgggc aatccttctt ttttttgtta
     3541 tgatacatgt gaaatctttc gtaaaggaga aagaaaatgc gcaaagctca aagacatttg
     3601 ctgattaaac aagtcattga agaattcccg atccgtacgc aagaagaact attaaacaaa
     3661 cttgggcatt atggcgttac cgccacgcag gcaacgattt ctcgagatat ccgtgatcta
     3721 aaaatcgtga aggcacctga tgaaaatggc gtttcccgct tcgtattgtt tcgcgaaaaa
     3781 ggaactcctg agactaggac tgaggaagag gatcggctgg ttcaaatgat tgaagacatt
     3841 gtattgaaag tcgaacgagt ccactttttg acgatcgtca ataccttgcc agacaatgct
     3901 catttatttg catctgtttt agatgagatc aaacctccct ctattgtcag tacaattgct
     3961 ggattcgata cgatcatggt catctcggaa aatgaggagg acgcaaaacg agtagaagaa
     4021 tttcttcacc atccaactga agtatcactt ttttaatcag agcacgtatt cctaatatgg
     4081 aatgcgtgtt tttttatttt caatattttc cttattgaat aaacctttac actctttaca
     4141 aaaatacatc attttttatt caaatttttc aacaccttga ttgaatcaca ataatcatca
     4201 tttatattgg atgttaacaa gagcagcttc ttgctctttg tgtatttcaa aaaagaaatg
     4261 aaaggaaatc atatgtataa attattaact tctggaccat tgacgacttc tgaaacagtg
     4321 aaaaatgcta tgctgatcga tcattgtact tgggatcaag aatacaaaga aattacacaa
     4381 tggataagaa aaaaattatt ggatcttgct caagtatcgg aaggaatata cacaacggtc
     4441 ttaatgcaag gtagcagctc cttcggggta gaatcggtat taagttctgt gatcaggcca
     4501 gaagatactg tactgatttg ttcaaacggc gcatatagac aacgaatgat caacatgtgc
     4561 gaatgtctga aactgaacta taccgtttat gaggtatcgg aacatcaaat cctccaagct
     4621 catgtgatta cacagctgat tgaagagaat ccgtctatct ccgcactagc aatggtccat
     4681 agcgagacta cttccggtat tttgaataat ttggaagaga ttgctgctgt cacacaagaa
     4741 agagatcttt tgtttatagt ggacgcgatg tcaagctttg gtgggatacc gatttgtgta
     4801 gccgaactag ggattgattt tttgatttcc agctcaaaca aatgtattca aggtgttcca
     4861 ggcttttctt ttgtacttgc taataaagcg gaactggaaa aacgaaaaaa tgttagccga
     4921 agtttgtcac ttgatctata tgaccagtac cgcgtgatgg aaaacgagaa tggcaaatgg
     4981 cgtttcactt cccctaccca tacggttttg gcttttcaac aagcgctgaa ggaactagag
     5041 gcagaaggag gaatcgaaac tcgatattca cgttatctcg ccaataatca tgtgctacga
     5101 caaaaaatga cagagatggg gttccagcct ttcattcaag aatatcaggg gccattcatc
     5161 acctcttttc tttaccctaa ggactggcga tttgatttta atcattttta tcagtattta
     5221 aagcaaaatg gatttgtgat ctatcccggg aaaatcagcg atcatgaatg ttttcgtatc
     5281 ggaaatattg gcgagatcta tccggaagat attttggaac tgacagcaat tatcaaagac
     5341 tataaggaga acgttaatga ttaaaactgt aatttttgat tgggcaggaa caactgtgga
     5401 ctttggctgt atggctcctg tccacgcttt tagaaatgct tttctagaaa aaggaatcca
     5461 actgactgac aaagaaatcc gctaaccgat gggaaaatta aaatgggatc atatccaaca
     5521 gcttctttcc ctcccttctg tcaaaaaaca atggatagct atttatggac aactacctca
     5581 ggaaaaagag gtggaggaat tgtatcaaca atttgaacat tatctgttta aagatttggt
     5641 caagcattct accttgaaac ccgacacatt ggaaacgatc gaacggttga gaaacgctgg
     5701 gatcaatatt ggttccacga atggatttaa tgctcctatg atgacaatcg tagcagaaac
     5761 cgcaaaaaaa gaaaggtatt caccagactt catggcaact tttgaagacg tcgaagggta
     5821 tggtcgaccg tttccttata tgatccataa aaacatgcag tacttcaaaa acaagtcggt
     5881 tgatgaagta atcaaagtcg gagatacggt ctcagatatc caggaaggga aaaatgccgg
     5941 agtgttgaca gtaggtgtaa ttgaaggtag ttcgctaatg gggatttctt atgatgagta
     6001 ccaaaaatta gagatgggga aaaagatcgt ttaaaacagt ctgttgctct tagattccaa
     6061 caagccggcg ctgatatttg tgtaaacaat ttaaaagagt taacagaata cattttgtac
     6121 gctgacatga atccagaatg acttttatac aaaataagag gttgtgaaaa agctgtcctg
     6181 tatcaagtaa taaaaacagc caagcaaaaa tagctcctca tatttttcgt ttggttaggc
     6241 ttattaccgc agatgcaagc ttttgaacac cgtttattcg aaaaaaaaaa gggagctgtg
     6301 acacgacttt tgtcacagtc cccttatttg caaacaaaca gtgtttaaga gacaacctct
     6361 ccactaaacg gtgttcattc agctgactct cggtatttgc tcaaaaaacg ataaaatttg
     6421 aaaagctatt ttctcttttg tttctctaat actcggatca gaacgctgat ttcacattct
     6481 ctttttctta catcttatct ggtgcatgaa gtcctagcaa acgaaggtct tctttcaata
     6541 atacagtcac tgcataaact agagctaagc gtgcttcttt ttgttcatca tcagctaaaa
     6601 tcttcgtatg agcatagtat ttgttgaatg cttgtgctaa tttgattgca tgtttcgcaa
     6661 tcactgacgg ttcgtatttt tccccagcag acaagaccgt ttcagggtat ttttgaacta
     6721 atttaaccac ttcccagctg gtatcgtcat tcaatgcata gtctgctgtt tctgaaggga
     6781 caaatcctgc tttttccaac aggctgactg cacgagcatg tgtatattgc acataaggtc
     6841 cagtttctcc ttcaaatcga acaacttctt ccaggttaaa gtcaaatgta ttcagtcgat
     6901 cgtttttcaa atcgtggaaa atgactgccc caaccccaac ttgtttagct acagcatctt
     6961 tgttttccaa atcagggttt ttctcgctga tttgttcttt agcagattgg attgcttcat
     7021 ttaatacttc ttctaataag acgatctttc ctttacgagt tgataatttt ttaccacctt
     7081 gcgtgatcaa accaaatgga atatggtgca tgtcgtcaga ccagtcaaag cccatttcct
     7141 tcaagacagc tttcaactgt ttaaaatgat agctttgttc attccctact acatatagag
     7201 attgtttgaa gtcgtaagta cgtttacggt acaatgcagc agctaagtca cgtgtaatgt
     7261 atagtgtcgc accgtctgac ttcttaatca acgctggatt caggtcataa gcagataggt
     7321 caacgatctc tgcaccttta tcctcgttca gcaaatgttt ttcttctaaa agtttgacga
     7381 tttcatccat cttgtcattg tagaaagctt caccgttcaa cgaatcgaaa cgtacttcca
     7441 gcagatcata gattttattg aattctttca tcgattcatc acggaaccat tgccataatt
     7501 gaatagcctc tttatcgcct tcttccagac gtttgaacca agcacgagct tcttcattta
     7561 attctggttc tgtttcagct acttcatgga attggacgta taaacgaagc aattcattga
     7621 ttggttctgc tttaacagct tcttctgtac cccatttttt gtaagcgacg atcagttttc
     7681 caaactgtgt tccccagtca cctaaatggt tgatgcggat tggctgataa ccgatttttt
     7741 ccataatgaa accgatcgaa ttaccgataa ctgtcgaacg caaatgtccc attgagattg
     7801 gtttggcaat attaggagaa gacatgtcga ttggtactgt tccttgattc ccgatgttgc
     7861 tatccccata atgttctttt tctttcacta ctgtttgcaa aacttttttg ctgataagtt
     7921 ctttattcat gaaaaagttc aagtaaggtc cgacaacttc gattttttca aagttggcag
     7981 aatcgatttt ttctgctaaa tctgcagcga tttgctgtgg cgcttttcgg taaacttttg
     8041 ctaatgaaaa ggctggaaat gcaacatctc cgtgttcagc tgattttgga ttttctagta
     8101 attgctcgac ttgttccagt gttaaatcat ctttgacagc gtcatagaca gcttttgcga
     8161 caagatcttt attattcatc tttattcctc ctaaagtgtt cccttgattg ttaaacaaaa
     8221 aaaatctcta gcgacggcat acttagtatg ccatcactag agacgagatt tcccgcggta
     8281 ccactccaat tgtcctaaaa aaggaccagc tccatctgat atcggtagac gaccgtctgt
     8341 ttaaaacaga attctccaag tgcgcttcgt tgtatcttct tcattcggct tccaccactt
     8401 ccgaattcgc tagccaagaa tgatacaagt actctcttgt tcaacaaatc ttagtcttat
     8461 taaactttct tgaattatat cagaaaaaaa aacgaaaggc tagtcgtttc ataaaaaaac
     8521 aatccagatg gcctaaagga gaagaaatac cacctggata gctataaatc atttcgtaaa
     8581 tacgaaagac ttaacaaatt aatcagctac gataatcgtt ccttcttcag aagctaaaag
     8641 attctcgata ttttctaaag aagtgatgat tgccttagcg tttggttttg cttcaacaaa
     8701 tgcaatagct gcttctactt tcggaagcat gcttcctgga gcgaattgct tttcatcaat
     8761 gtattgtttc atttctgaaa cagtaacagt ttctaatttc ttttgatctg gtttttggta
     8821 attgacatac acattgtcta ctcctgtcaa tacgatcagc aaatccgcat cgatgatttc
     8881 ggctaatttt tctgaagcaa aatctttatc gattacggct tctcgtcctt ctaatccagt
     8941 agctgtttct acaacaggaa tcccaccgcc accaacagag actgtgataa ctccttgatc
     9001 gaccagcgtt tcgatgacac gtgcttcttt aatcgagatg ggtttagggg aagcgactac
     9061 ttttctccat ccacgtccag catcttcttt gaatgtcaca gtggagtcag cattcatttg
     9121 ttctttggct tcttcttccg tgtagaatgg accgactggt ttacttggat ttttgaatga
     9181 aggatcattt tcatccacaa taacttgagt gactaaagaa accacatctt tatcgatccc
     9241 tttttccttc agtacttcgc ccatggcgtt ttgtaaccag taaccgatag atccttctgt
     9301 cattgcaaca catgtatcta aaggcattgc cggtgttttc tcagaatcag cagcttgctg
     9361 ctgaatcaat aggttaccga cttgcggacc atttccgtgg gagatgatca attcatctcc
     9421 ttgttcgata aatttcacta aatactttgc tgtttccatt aacgcttctt gttgggcttt
     9481 tgcacttgca tccgtagaca agatggcatt gccccctaaa gcgacaacta cttttcgttt
     9541 ggccatttca ttcgtccttt cttcagttct accgaatttt ctagataaca aaacgagcca
     9601 gcgttttgtg attttccctc aatgctgact cgttttttcg tgagatgaat gtggttatgc
     9661 ttaaacgcga gggataaaca agttacctaa tgttgctgcc atgatcgctt tgattgagtg
     9721 catacgattt tctgcttggt caaattgacg agcatatttg ctgcggaaga cttcatctgt
     9781 tacttccatt tcagtgatgc caaagcgttc tttcatttgt tcaccgtaaa cagtgtttgt
     9841 atcatggaaa gctggcaagc agtgcaagaa gatcaaacga tctgtgttgt gtgttttttc
     9901 taccatttcc atattgattt ggtaaggttt caacaatttg atacgttctt cgaatttatc
     9961 ttcttcaccc attgataccc atacatcaga ataaaggacg tctgctccgt ctacaccttt
    10021 atcaacatcg tcggtaatca tcaattgtgc accggatttt tcagcaaagc cttctgccat
    10081 tttgacgatt tcttcttcag gttgtaattc ttttggtgca acgatacgca tattcgctcc
    10141 taaaattgca cctgtgacta ataaagagtt agccatattg tttcgaccat ctccgcagta
    10201 agcaacagtg ataccttcta ctgttccaaa gttttcttgg atagttaaaa agtcagcgat
    10261 catttgtgtt ggatgccatt catcagtcaa gccattccaa actggaacac cagagaattc
    10321 tgctagttcc tctaccattt tttgactgaa tccacggaat tcgattccgt caaacatacg
    10381 tcctagtact ttagctgtat cttctgtgga ttctttttta cctaattgga tatcatttgc
    10441 acctagatat tcaggatgtg cacctagatc gatcgctgca gtagtaaatg cagaacgagt
    10501 acgtgtagat gttttttcaa ataaaagcgc aatatttttt ccttctagat agtgatgtgg
    10561 gataccacgt tttttcaagt ctttcaagtg ttctgaaaaa tcgatcaaat attgtaattc
    10621 ttcttttgta aagtcttttt ctgctaataa gcttcttcct tggaatactg attctttcat
    10681 attaaacatt ctcctttaga ttgtttttat ttaagattaa agaggaaatc ccgacggcag
    10741 ctgacagtaa gactgccgtc aggaaacaaa ttacttcaag tcttcacgga caagtggttg
    10801 gctcatgcag cgtggaccac cacgacctcg tgacaattca cttgaattga tttctaacac
    10861 tttgatgcca tagctacgaa gcaattcgtt cgatacataa tttcggttat aagtgactac
    10921 aacacctgga gcgattgcca atgtgtttga tccatcattc cattgttctc gaggtgcaac
    10981 gatctcatct ccattaccag ttgggatcag tacaagatca tctaatccaa gagcagcttt
    11041 catcgtttcg tgtaagttat ctgaatgagt aatcttgata tcatcaccat ctggttcgat
    11101 tgtaaagaca tccatcttac cgtctttact ttgaattgct ggatggatag tgaatttatc
    11161 gtgatctacc attgtaaata cggtatccaa atgcatcatt gcacggttgt tcggaatttt
    11221 gattgccaac actttttcaa atcctgaatt ttttgcgaat aggtggcgtg ctaatttttc
    11281 taaagctttc gcattagttc gttgagaaat cccaacagct actactttat cgcttaagat
    11341 caattcgtct ccgccttcga tatgatctgg attttcacga tccaaccata cttcgattcc
    11401 tttatttgca aatcttggat gatgtttcaa aatatattct gtaaacatcg attcacgacg
    11461 acgtgcttca aaagtcatct tattgactgt catcccattt cccattgatg cggaagggtc
    11521 gcgtgtaaag tacaagtttg gcattgggtc catgtaaaat ggatagtcat cgtctgctga
    11581 cagatcatat agactgctag aacgtacttc gatatctttt gtacgaaccc cagccatgat
    11641 tttatctacc atttcttgcg tttccattga tagaaggaac tcatgaagac cctctcttac
    11701 agcattcgat gcaatatgag attcatccaa cattttattc aagaattgtt ctttgacatt
    11761 gcctgcgtca atcgcttctg ccgctaattt ttccaagtaa agagtctcga cgccttcatt
    11821 ttgcaatgtc ttcgcaaagt tgtcatgttc ttcttgagca atcggaagat atggaatatc
    11881 atcgaacaac agacgatcca taatatctgg tgtcaaattt tccacttctt gtccaggccg
    11941 tttcagtaaa actgtcttta atttccctat ttcagagaaa acgtgaatag gtttatccat
    12001 gttgtgttgc ctccttcttt ttgattacat gtataggata tcctcctttt gtaatcactt
    12061 tcataatttt gtctcattca aaatgagaca ttcctcacat ta
//
LOCUS       DACCYH010000089        12024 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.093, whole genome shotgun sequence.
ACCESSION   DACCYH010000089 DACCYH010000000
VERSION     DACCYH010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.093"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(233..958)
                     /locus_tag="IVY85_002316"
     CDS             complement(233..958)
                     /locus_tag="IVY85_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045318.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1112..1438)
                     /locus_tag="IVY85_002317"
     CDS             complement(1112..1438)
                     /locus_tag="IVY85_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045319.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(1475..1840)
                     /locus_tag="IVY85_002318"
     CDS             complement(1475..1840)
                     /locus_tag="IVY85_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP8045320.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(1877..2092)
                     /locus_tag="IVY85_002319"
     CDS             complement(1877..2092)
                     /locus_tag="IVY85_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8045321.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            2221..2556
                     /locus_tag="IVY85_002320"
     CDS             2221..2556
                     /locus_tag="IVY85_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8045322.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(2632..4629)
                     /locus_tag="IVY85_002321"
     CDS             complement(2632..4629)
                     /locus_tag="IVY85_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045323.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(4727..5002)
                     /locus_tag="IVY85_002322"
     CDS             complement(4727..5002)
                     /locus_tag="IVY85_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8045324.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            5234..5968
                     /locus_tag="IVY85_002323"
     CDS             5234..5968
                     /locus_tag="IVY85_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8045325.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            6007..7149
                     /locus_tag="IVY85_002324"
     CDS             6007..7149
                     /locus_tag="IVY85_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8045326.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(7777..8262)
                     /locus_tag="IVY85_002325"
     CDS             complement(7777..8262)
                     /locus_tag="IVY85_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HAP8045327.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(8264..9028)
                     /locus_tag="IVY85_002326"
                     /pseudo
     CDS             complement(8264..9028)
                     /locus_tag="IVY85_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9198..10391)
                     /locus_tag="IVY85_002327"
     CDS             complement(9198..10391)
                     /locus_tag="IVY85_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8045328.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(10475..10678)
                     /locus_tag="IVY85_002328"
     CDS             complement(10475..10678)
                     /locus_tag="IVY85_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP8045329.1"
                     /translation="MMNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVI
                     MNGNRIQIKRKQFEKIIDTLDTI"
     gene            10935..11177
                     /locus_tag="IVY85_002329"
     CDS             10935..11177
                     /locus_tag="IVY85_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045330.1"
                     /translation="MAHQPSGSAQTECVRMPYDAPPLSCGHIYQGAIRPPGKHRNGER
                     VSRFMLPGPVRQFVTIAWMSLISRRRFMASSRHTCG"
     gene            complement(11099..11332)
                     /locus_tag="IVY85_002330"
     CDS             complement(11099..11332)
                     /locus_tag="IVY85_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8045331.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(11339..11770)
                     /locus_tag="IVY85_002331"
     CDS             complement(11339..11770)
                     /locus_tag="IVY85_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8045332.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         3681 a         2466 c         1942 g         3935 t
ORIGIN      
        1 aaatttctta aaaatttttt cactaaaatc actacctgaa acagtctaaa acacttatat
       61 atcaacgttt aatgaaacta tctccgtttc atttcagata caaataaata tagtcgggct
      121 ccaaatccga aaaaaaatac ccccagtagc ttatcctaag ctactggaga tgtataagta
      181 cggtcatata acaacggggt agggtaaata aatgaaaaag atggaagaat cattattcaa
      241 taactttttt aaatacttcg ttgtaatcag ttacctgtcc gttcttaaat accacagaat
      301 aatctttttt cccattttca agtaaccgaa gtggaatatc tttaccaact ttctctgtca
      361 ctgcattact gatacctgtt agattagcta ataaatctga taaagattga gcatctgtag
      421 aaatctctgt atcagtattt atcgtgattc ctttatcttc atttacaagt ttccaagtag
      481 gtggtatagc tgtgtatgtt tctacaaatg aattaagttt ttcattaagt gacttctctt
      541 ttatatttcc atctaaaaac tctgtatata tggcttgagc tatactaaaa caaagctggc
      601 cttgttctaa taattttaca tcattagtta gtaaagctgt gttcacgtta tcaatcgctt
      661 caagaagagt atctttggtg tccctaacag ctttaggtgt gccttctgga atttccctac
      721 ttgatacctc gtccatttta tcttccataa accccttaaa atcatatctt tctcccaaat
      781 ccatagaagt ttttgagaaa gctttcattt ctgagtctat ttcttcaata atatttaaca
      841 tgtctgatac atattgttta ggctgattcg tttttaatct atatgtggta aatatctcaa
      901 ttcctccaat tatcaataaa attgccaaac aaatccataa taattttttc tttttcattt
      961 ttcctcctat acactgctgt tataacagtg actaatgtaa agattttccc agcaatcatc
     1021 agactattat ttacagggaa tcaaaaaaac tccttagtaa gcttacacct ttaggagttc
     1081 ctgtttgaac ggtatgcata atacatttaa atcaatttaa cattaacacc tcagtatatg
     1141 gtatctctac atatacacgg tctttttttg gatagtactc gacatttata ttgatagcac
     1201 ttccattgtc taacgtagct ttagcccggt acgtgttatc accattatca tgagtgatcg
     1261 tcacatcatc acatgtccga tttaagttat actgttcttg taaaatctga gttaccaatg
     1321 gtacagatga cttctctagt ccagatgaac ccgtcattct tgaaaaaatt atacctataa
     1381 tagccaatac aacgatacct ataatgacaa acttcttatt catatttact ttattcaact
     1441 ttttatctcc tatctccaaa atgattttct ctcatcagta actcattgac atccccaata
     1501 gtacaccaaa gatggctcca gctacccctg ctatcatcaa aatcgtaccc atttgttcat
     1561 ctttctctcg atataagtaa cccataataa cagcaacagc accaaatacc acaggaataa
     1621 atgctaatga tactaccata gaaatccaac caataatcaa atacagcttc cctgtctctt
     1681 gtccatgtac gatttgttcc ttcatttcat taattgtctg ctcagtactt ttctttactg
     1741 tgtctgttcc ggttgtctca tctgtttctt gtaatgctcc gcaaaactgg caatacttac
     1801 tttcatttgt tatttcttta ccacattttc tacaatacat gtcttctcct cctaaaatca
     1861 ttcaattgct tattagctaa ctgtttactt tattaaatac atcagaaaac agctcattta
     1921 ttggtacact taatatctca gaaagcattt tcatttgata gggtttagga atcgttctcc
     1981 ctgtttgcca cgaacttaca cattgctgtg taacattcat ttttttggct aactcagttt
     2041 gggaaagtcc tttttctttt ctgtatcggc tgagattact tacttttttc atgattctcc
     2101 cctcctgttt tattatatac aacaatatga tgtatttcaa ctcataaaat atataaatac
     2161 ttcaaatgtc attacaaata caacaacttg ttgtactcta ctgagaaggg gggtgtatta
     2221 atgaaacaga agcaatatgc aaaggtcatt agagaaaagc gaaaagcact cgggtggaca
     2281 caaaaagaat tagctaaaaa aatcttctct acacaacagg cagtagcaag atgggaaaat
     2341 tctgtaacag aacctaacct cgatagctta acagctcttt ctagagccct aggaacccct
     2401 gtaagccatt ttctagacaa tgttgacata gattatgagg aagagttttt ggctctctat
     2461 cgctctctta gtactgaaga tgcaattcga acaattgact atatgaagct attaaaaagg
     2521 caagaaaatg aacgcaatca gctattgaaa gattgaaaaa taaataaccc accgaacttt
     2581 aacggtgggt gtattgagat gaaataagat taagagctat ggataacctg tctagtttaa
     2641 tataattggg tctcttctat tccttactct ccgtccatca tctcttgtta cacttgtaaa
     2701 ttgataaata ggaactaatt caactcctga taatatcaat tcttttatta cttcatctat
     2761 ccctgcccct ttcgctagat atactaaaaa actactgctt gctttcacat tgtatagttt
     2821 cttaatatcc ttagaagctc gttcaagagt gaatccttgt ttaacaaagt agcctctgtt
     2881 ttctattaaa gcatttttca cgctgttatc cctgacattt cgtaggcgtt cacaaacagt
     2941 gttaataagt tctttttctg tcattagata tgtcccttgc cacgataagc cctgtaaaac
     3001 ttcatgtgct tcatctaaaa tcaattgttt ttccttgcta agttctgatc gggaattttg
     3061 aggaaatact cttttagcct ctttttcgtt aaaacatatc ttaacccatt cataagaaaa
     3121 atattgatat gtaaaatgaa atttttccag aagtggaagg atatcttcat tcaatttttg
     3181 aatcacagaa gttgcattaa aaacgtctaa tcgatagaca ttgcttctct tatgtttaaa
     3241 gtttcctaac gctttctctt cctttaacct atttaggtat tcgagcatct tcaaaggaac
     3301 atcttcatca tctaattttt ttatcaatcc tataaaagtc ataagtgata gtaacttatt
     3361 acatttatct atgtcataag ttttaccata gtaagaactc atttttttag ctaattgttc
     3421 ccccgacaaa atacaagtca cactttcctt actatcatca aaaaaattaa catcagaaag
     3481 caagacatca tagacacgtc gcatttcctc tagataattc cttattgtcc tgaaaagttt
     3541 gtctttcatt gattcaagag tgtcaaaaag tacttttgat tcctctttca attttcgaaa
     3601 atgtgtagga tacaagtcta ggttctctaa tagccaatta aatgtttcta cccgactgtc
     3661 tttctttaaa attagcatca caactccaat aaaattttca ctgatatcac aattactgga
     3721 atgacaataa aaaatggaat gcccagtttt actggtaaaa atactagcag agggattatg
     3781 atcgttgtga aacagacatc ttaagttttt acttgactta agtaatttat tcatatcaat
     3841 ggttaacaat ctttcataaa tctcattaaa agacatgtcg gaggaatcaa acaggcagtc
     3901 tttaaaccac tgtttcattt cttctttgtc gtcatttttt atcaacttaa caaaatcggt
     3961 ttcattgact agttcatact ctctccctga atcactttga caatttttag aacatgtcat
     4021 ggtaaaatca atcggaagct cagtaatatc taacatgttg tcttcattaa ttattatcac
     4081 tgcatctgac ttatatcctc caaaaaacat tctattcgaa gcatttacat tgtcatcaca
     4141 cccggggatg ttctttttca agtattcgta aacagcaacc attttaccat agtcagtaat
     4201 tgcttctttt aatagaaaga caatcttgta tttttctaga ttttcagagt accgaatact
     4261 tttttgtatc gcaaacgcag aattatgaac taaattgata gataaaacct tttctatgct
     4321 taagtatcta ctatcatctt tactcaaatc tactaccttc cccttctcat ctttaacagt
     4381 gttatcgaca tccagaataa ttactctggt ttccacaaaa ctctccttgt cattgccttt
     4441 attctttgtc ttgcttggaa taaatgattg tgatgaattt agtatctcta caaactcatc
     4501 tcttgtaact tctctatagt tatctaaaca cctatctctt acatcagttt tttctttttt
     4561 tgtaactggc ttctctaata atttttcttt atctacataa attggaatat taatcattga
     4621 tgtttccatt tgtgtttcct ccttataaaa ttctgttaaa gactaaaaca tacagataat
     4681 aaaaacaaac tctttattac tatatgtttt agtcttattt gtttactcaa cctttatacc
     4741 gttccatgaa actatcaata tcagattttt taaatctaat gaccgtatca atttttataa
     4801 caggtaaccc gtattgagaa acatattttc taatcgttcc tactgataca cctagatatt
     4861 cacttgcctg ctgatagtcc atatactcta gagtgactcc agaaataatg tcttctagca
     4921 taggctttag cactgtaatt atctctttca cttgttgttc aacttgtata gaaacaatgc
     4981 tatcaatgat ttgtagagac atatttcccc ctcctttcgt gattgcttga acaaattttc
     5041 taagaattat cttcgtattt tttgctcaaa ataaaagtat tttgttatta accgattaat
     5101 caaacaaaaa atgattacca gttcatcgac atacacataa tacctcgata aaaaaggggt
     5161 gtcaatagtt ttgtaaaatt tatttacaac accccttttt tgaggtatac taattttagg
     5221 aggtgtggtc aatatgataa aacgtccacc aataaattat ttagaacgaa aaaagatact
     5281 cggaacaaaa ataaaagcta taagaaagag taaaaaatta acacaaccag catttggtct
     5341 tatgattaac aacggtcaat taattgacaa aaagactatc tacgaatggg agaagggaac
     5401 ctatttacca ataccagagc gattatcccg aattgctgat ttgggaaata tgagtataga
     5461 ggagttagtt tgtggcaatg tagaagaata tattttggga attattcttt atagagatag
     5521 cattgtacta gatggtataa catttccaga taaaaacctg ttccagcatc ttcgtcaaca
     5581 gttcccacca gtacactcta atttagatac atggctggat agatattcca aattagaacc
     5641 agagatgcaa gaatttattg ctaacaagac ttgtaataaa gttaaaaatg aaaaaattag
     5701 tcttttcaat atcttaaaaa tagaagaatt attcatcaat gctattgtag aagaatttga
     5761 caataacatt cttttcctca ctagcagtat agaagaacta ctagaaagaa tggtagatga
     5821 atggctacct attcaattga aagacatgtc ttatcccgaa gaagctgtga gagaaattac
     5881 tgataatata aacaagctag aacaaacgat ttcatcaatt gggaaaaaat acactaagaa
     5941 aaaaatgaag ggaggtgata ccatctagtg gtaagtactc cttgtaaact aagaggagat
     6001 gatttcatgg caaaaatatc aaagtacaaa aagaaaaacg gtcaagtggc gtggatgtat
     6061 aaaggacaca ttgccaccga tccacgaact ggtgaaaaaa taaatactac gagaagaggt
     6121 tttagtagca aacaagaagc tcaaaaagat tatgacgagt acagacatca aatattatac
     6181 ggtgttaaaa agaaagcacc tgatatgaca tttgaagacc tgtataatga atggataact
     6241 catcagcgta ccagtgttaa agcttcaaca atcgcaatct cagtaagata cgcaaataat
     6301 caaatacttc cagcatttgg taagcttaaa ctatcgaata tctctgtccc atattgtcaa
     6361 aaagtggtag atgaatggca cagtaaatat gaaagttacg attatatgag aaaacagact
     6421 gcacaaattt tacgctacgg ggttgcaatg caatatattg acaataatcc tatggaaaaa
     6481 acacttcttc cacgaaaaaa agagtatgag aaaaatcgta aattttactc aaaagaggag
     6541 cttaataatt tactggatgc ttttaaagat tttggtaaca tgaaacaata cgccttcttc
     6601 agattattgg catacacagg tatgagaaag agtgaagtac tcgctttaca gtggaaagac
     6661 atagacacgt ttaataaaga actacatgtc aataaaactc ttgctgtaga tgaatttggt
     6721 aaagttataa ttcaatctcc gaaaacaaga gcttctagac gagttatctc tttagatact
     6781 gagactcttt ctatcctcaa caactggaaa ttacagcaaa aagaagagta tttaaaactt
     6841 ggatacaata ctagctctaa agagcaacat gtttttacaa cagttaaaaa cactctatac
     6901 attccaaaca ctgttaatga ttggttaaga tatattttga aaaaatataa cttgcctcgt
     6961 attacacctc atgggtttag acatacacat gctagtttat tattagaagc aggagaatct
     7021 gtcaaggtag ttcaacaacg attagggcat gaaaatagta aagtgacatt agacatctat
     7081 gcacatatta caaataatgc acctaagaaa acaggtcaag actttgctga tatgatggct
     7141 catcagtaaa agagctggtc aaaatctggt catttctagt atacaggcaa aacaaaaaag
     7201 ctagaaccct tatatatcaa gggttctagc tcctataaca ggtttaccca acactgcctt
     7261 ccaatgactc ttttgggaat cagaggaaag aatcaggctt tatagagata tttcatctaa
     7321 caaattactt tagactgaca gtatatatca gcaggtactg tcatcgggaa cgtagccaat
     7381 atcgtatcca aaacagcagg tagagaagct atttgaggat tcaagttatt ctatttcaac
     7441 catgaagtta agttcattaa aaataaagat agttggtatt tcaataattt ttgtatagag
     7501 ttaatcactt tattgatgct aattcatctt gattggtatt ataatcgtat tcaagttgcg
     7561 tggcgtgctt ttgctagaaa atatataact gttctttata aagaaatata aggattaaac
     7621 attttttatc atagagtttt acttatctat agaattatgt ctgttcctaa ttcagtttga
     7681 tgaattaact gataaaaaaa tatctactat ttaaagatac agggataagt aagtaaaaaa
     7741 ataacatgtc attagatagt agacagttcc aactctttat ttaccttttt tgtatgctgc
     7801 actgtcaatc caagttccta aattgttgta tccatcatca cttatataat catattggcc
     7861 atcaataatt tcgtcaaacc acaaatattc tccatctggt ttttgcccaa ttattttaat
     7921 tactgattga ctagtagttg tcatctggtt aaaatctttt attttaccta tattaacggc
     7981 caaaatagca tttcctcgct tataactctc ctggatttca tatttaacat attctcgatc
     8041 aagagtttct ttacctatca gcaccacagt cactgatgtt ccaattaatt gattatctat
     8101 ccagtttttt atagcaatat ctccattacg tttaactttc tcaaattcag ccttatctac
     8161 aaaaccagct tcctttgttc ctcgcaaagt ccagctgttt ctaactacca ttgctcttgt
     8221 tacatctgca taatggaaac taaaaaaaac ccttctcacc atattaatcc tcctgaatac
     8281 tatttattaa actgacaatt tgttcaactt ttttatctac ttctccttca gtatctaatt
     8341 tttctagcgc ttgaaattta ctttttaagg aatcatcaaa ataatcactg aaattctctt
     8401 gaatcttatc aaacaaaacc ttacttgcac ctttggtaaa tccaatcgga actactatat
     8461 tttcattttg aacagataat tcaaattctt caatcattcc atctgctgta actatttcac
     8521 ctgattcaag tttgtttcca aaaacaaaaa tactaatccc agactctgca ataatgtttt
     8581 ctcgatatct tctccactca tgaggcatat caaggggaag tggtcttaaa gaaagtacgt
     8641 cttgtatgtt attttttctt aaatttcttc tctgctttag aactcctgaa acaatactat
     8701 taccaattcc aagtccgtaa cctgatacta ttctataacc ggattcagaa agcctatatg
     8761 ctaatttctc gacaaatttt aatcctttcg attcagatgt aaatggagta taatcaacag
     8821 cacttcccga aataaaaata gtttttctca aatacttttt tctaatactt ttcaaaatat
     8881 cagtaatctc agaataatca tctacttcaa caacgtttat cgaaaatctg gacaaatcat
     8941 tgatttgtag tcttcttttt gtcagttcat aattatatct ctcttgatca aaaaaatcct
     9001 cagaatcgac aaaattttcc tttaaaatta tatgggattt tagattatct ttttagaagt
     9061 tcttgaatgt cggtatagcc cgtgtttccg ggcttttccg gcatttttga tattgttaga
     9121 tacttacaaa tgttcttatt acctctcacc atttccccct caaaagtagt aaatggagta
     9181 gtaaaaccgg ccttgtgtca ggctgccagc cgttccattt ctgcccttgc ggaatcgaat
     9241 gtggcgtgtg cataatagtt cagcgtcatg gtgatattgg aatgtcccat aatgtactgc
     9301 aaggctttgg ggttcattcc cgcattggct aaccgggtgc agaatgtatg acgcatagca
     9361 tggggcgtcg tcgttttcgg caacgcttct tcatggctct tgttgtactt cttcacaagc
     9421 ctgccaaaca tcccgccata atctaccgcc gtcatgggat agccgttctg tttgagaaac
     9481 aggaaattgg catacccgtc aataatgaac ggctttgcac ctttgcggtt ttttatcact
     9541 cgctggaacg cctgatacac ttcctcattc atggggattt ttctgacgcc gctttgggtc
     9601 ttgggttcgt caatatagta accgacgccg gtatttctca aaagctggtg gctcacgatg
     9661 attacccggt tttcaaaatc caaatctttg tccgtcagtc cgcacagttc cgaaatgcga
     9721 agccctgtcc ccagcagaat aacgattgcg tcataatgct tttggtaaac cttatcacct
     9781 tgaataaagg acagaaaact ttcttcctgt gctggtgtaa gaggcacctt tggttccgta
     9841 tcgtcgtcca gaacgtcact aagctggaag tcaaacgggt tttttcggat gcaatcgtcc
     9901 tgtatggcgg tgtaaaaagc cgctttcagg gaacgcttgt cgttattgat ggtcttataa
     9961 gaaaggccct tttctttcat ccgaagcgcc cattcctttg cgtcggacag cttcacactg
    10021 tcaatcgggc agctccccag ccgatcttgt tccagaatac gcatcagccg ttcccggccc
    10081 agtttggtgt tgtgcttcac attgccccgg tgcctgatct gcttggcgta gagctggcag
    10141 acggtcattt tcttgccgat ggtgtctatc ccgtcgtcaa ggtctttctg gatttctttc
    10201 tccttttccc ggagtgaaat atcctcccgt ttacctgccg gggtcttatc cgtaggcact
    10261 aacttccatg agtaaacaaa ctgcggtttt ccaaaggtat caacatattt gtaagcatat
    10321 cgtccgtctt ttctctggct ctctccagac cgcaaaacgc gatttttgct gtctcgtctt
    10381 ttttctgaca tctcgtcatg ctcctttcca tgacggaaag agccgcatat atgcttataa
    10441 attattataa catatctgcg gccctttttc actattagat tgtgtccaaa gtgtcaatta
    10501 ttttctcaaa ctgcttgcgt ttgatctgga tgcggttgcc attcataatg acccagccag
    10561 aggccggatt ttcctcggcc agcttacgca atttgttttc tccgatacgg aaatatttag
    10621 acgcttcctc aatcgtcagc gtgtattttt cccaaatagg cacatcatgg ttcataatgc
    10681 agcccccttt cacaaattca aaggtttgtg tgatttaggg gcatagacgg acggctgctg
    10741 gcacagctcc acgggacttt cacccccgcg tggttctcac gcggccctac ccattgcctg
    10801 cgacgctttt aacgctcgga ctgtggctat aagggagtat catagtcctg cctgtacgtc
    10861 gtcgcccgca agccgccacg ctcacattgc ggcgtggtgt tcctcgctcc gcttttcccg
    10921 tagggaaaag tatcatggcg caccagccgt ccggctcggc acagacagaa tgtgtccgta
    10981 tgccctatga tgcgccgccg ttatcttgcg ggcatattta tcaaggtgcg atcaggccac
    11041 cgggaaaaca ccgaaacggg gaaagggtaa gccgtttcat gctgccgggg ccggttcgtc
    11101 agttcgtgac gatagcgtgg atgagcttga tttccagacg gcgcttcatg gcctcgtcca
    11161 gacacacatg cggatagccg ttttcgtcat agagcgtccg ggtacacagc ttgttcatgt
    11221 aaccgctgta atactgcaaa acgcgctcta cggctccggc atccccagcg cgggcggcct
    11281 cgatcaccga aaggggcaaa agctcaatgc ccttgtagtt cgggttcttc atccgcgtct
    11341 aacctccctt cggcatcaac gcctttagct gtttccgcag ctccgataag gtatttgtcc
    11401 tgtgccgctg aacggcactc cgggacattc ccacaaggcc gccgatctca ccatctgcca
    11461 tatccagaac acagtgcagg attaaaatac tctgctccat gctgggcagc gcggcaaagg
    11521 cttcggcaac aagctcattg ttgatatgta ggtcatagcc atgcgacgaa aacgtaaagc
    11581 tgtcgctggg gtagtgatct actgtacaga gcttgtccaa atctgcctgt gacaaagagc
    11641 ttagtgacac ttcacggtca cggcggcgct tcatttcagc caagtagctt ttggcctcgt
    11701 tacgcagtac cgtcttgcaa aaagcatcga acatgcaccg cgcttcatag tcgcggggga
    11761 tagcatccat cttctcaccc cctttctgtg ggggacttag gtggttctct ccctttccgc
    11821 taacaagact ttgacaaggc cgccagctac ccgctaaata cctccttttt gaaaatttct
    11881 caaaaaattt ttttggggac agccgggccg caaaagacga caaaaatcgc ccggcaggtc
    11941 gcaaacctac cgggcgagaa aatgctttgc tatgctgctg atttacatgg tatagtacat
    12001 actcataacc ggaatagccc tgtg
//
LOCUS       DACCYH010000090        11923 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.094, whole genome shotgun sequence.
ACCESSION   DACCYH010000090 DACCYH010000000
VERSION     DACCYH010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.094"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(29..1261)
                     /locus_tag="IVY85_002332"
     CDS             complement(29..1261)
                     /locus_tag="IVY85_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8045333.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1516..2085
                     /locus_tag="IVY85_002333"
     CDS             1516..2085
                     /locus_tag="IVY85_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8045334.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2263..2703)
                     /locus_tag="IVY85_002334"
     CDS             complement(2263..2703)
                     /locus_tag="IVY85_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP8045335.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2861..3625
                     /gene="map"
                     /locus_tag="IVY85_002335"
     CDS             2861..3625
                     /gene="map"
                     /locus_tag="IVY85_002335"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP8045336.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3657..4580
                     /locus_tag="IVY85_002336"
     CDS             3657..4580
                     /locus_tag="IVY85_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP8045337.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4805..5044)
                     /locus_tag="IVY85_002337"
     CDS             complement(4805..5044)
                     /locus_tag="IVY85_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8045338.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5049..5468)
                     /locus_tag="IVY85_002338"
     CDS             complement(5049..5468)
                     /locus_tag="IVY85_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8045339.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5695..6933)
                     /locus_tag="IVY85_002339"
     CDS             complement(5695..6933)
                     /locus_tag="IVY85_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8045340.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7040..7675)
                     /locus_tag="IVY85_002340"
     CDS             complement(7040..7675)
                     /locus_tag="IVY85_002340"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8045341.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(7789..7881)
                     /locus_tag="IVY85_002341"
                     /pseudo
     CDS             complement(7789..7881)
                     /locus_tag="IVY85_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(8017..8652)
                     /locus_tag="IVY85_002342"
     CDS             complement(8017..8652)
                     /locus_tag="IVY85_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8045342.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            9164..10306
                     /locus_tag="IVY85_002343"
     CDS             9164..10306
                     /locus_tag="IVY85_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8045343.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            10299..10505
                     /locus_tag="IVY85_002344"
     CDS             10299..10505
                     /locus_tag="IVY85_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP8045344.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            10584..11777
                     /locus_tag="IVY85_002345"
     CDS             10584..11777
                     /locus_tag="IVY85_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8045345.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
BASE COUNT         3854 a         2243 c         2193 g         3633 t
ORIGIN      
        1 tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta
       61 ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat
      121 ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg
      181 taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt
      241 catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta
      301 caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga
      361 catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca
      421 tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta
      481 ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt
      541 ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa
      601 tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt
      661 ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat
      721 agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca
      781 gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga
      841 ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat
      901 aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc
      961 tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta
     1021 cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag
     1081 tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag
     1141 caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta
     1201 cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca
     1261 taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta
     1321 tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc
     1381 atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca
     1441 tatcaaaaaa aacatgttac aatgaaatta atcaatggtg gaagcgttat aaaaaaatta
     1501 aggagattaa aaaatatggc aagaaaaaag acgattacta gagaacagat tttggcagct
     1561 gcttatgaag tagctgccac cgatggattt acacgattta ctgcgagaaa catcgcgaac
     1621 aaaatgaatt gttccacgca accgatttat cttgagttta aaaatatgga tgacttgaga
     1681 caagctcttt ttgagcagat ctatgatcat ttacaatatg aagtgttcac tgttgagcat
     1741 acagggaata cgattatcga tcttgctttt aattatatcc attttgcaag aagagagaag
     1801 aagttgtatc gttcactgta cttagaagaa tatggtggag gaaaagagat gcagaatttc
     1861 tcttatactt actttgtgaa tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa
     1921 gagcagatcg aatctttgta taatgggaca tggatcatag ctactgggat cgcagctttg
     1981 atgtcttcaa gcatcatcca tccaactgat gaacagattg aaaagatgat ccaagacagt
     2041 atagatgcta tcttgaaatt caaagaacca atcgaaatcg attgaatata ctcaaaagtt
     2101 ccacaaatca gtaggtaaca agagatgtgt caaaataagg aaaagttcgt ttttcttaca
     2161 gcgtatgcat ttttaactcc cgaagaactc ttccatgaat gcaatgaata aaagagccat
     2221 acagattgaa taaatctgga tggctctttt attgcaagta tattaaattt ttgcgactag
     2281 ttctttagcg aaagcttcta aattttcgat atcttcttct tcagcattca gatccacttt
     2341 aacattttcg gctccttttg atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac
     2401 cgatttgcag aattcatcgt aaaaagtatc accagatccg cagacaccgt aaatttttcc
     2461 caatagatcc aattcttgca agtcttcata aaagtctacg atctcatcag gcagatcgcc
     2521 atcatcgtat gtataagtag ctactacgca aatatctgcc tcctcgaatt catctgcatc
     2581 tacttgggta cattcgttga tttcgacttc gatatccaga ttttcaaatg cttctgcaac
     2641 aatatcggcg atttcttcag tgtttcctgt catgctagca tatacgattt ttgctaaagc
     2701 catgatgtgc ctcctcgttt ataattattt aacaattatg aattatacca aaacatcatt
     2761 gaaaagaaaa taaaacctag agacacctgg ggaatgtcat ttggctaaat gtatgccgct
     2821 atgttaaaat gagagggatt tgtaaaaagg agacgaaaat atgattactt taaaatcagc
     2881 acgtgaaatt gaaatgatgg cagaatctgg agctttatta gcagatgtac ataaaaatct
     2941 gcgtgatttt ataaaaccag gaatcacaag ttgggatatt gaggtatttg tcagaaacta
     3001 tatcgagagt catggcggaa ttgctgcgca aattggcttt gaagggtatg aatatgcgac
     3061 ttgtatcagt ataaatgatg aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa
     3121 cggtgatttg gtaaaagtcg atatgtgtat cgatttgaaa ggcggcattt ctgattcttg
     3181 ttgggcctat gtagttggag aatctacacc tgagatcgac cgattgatgg aagtaacgaa
     3241 gaaagctttg tacatcggta ttgagcaagc acaagtaggg aatcggattg gtgacatcgg
     3301 tcatgctatc caaacttatg tagaaggcga aaatctagcg atcgttcgtg attttattgg
     3361 tcatggcgtt ggtcctacga ttcatgaaga accagcagtt cctcattacg gagaagctgg
     3421 aaaagggttg cggttgaaag aaggcatggt tatcacgatc gaacctatgg ttaataccgg
     3481 aacttggaaa atgaaaatgg atcctaacgg ctggactgcg tatacaagag acggcggact
     3541 ttcttgccaa tatgaacata cattggctat tactaaagat ggtccaagaa ttttgacatc
     3601 acaaggagaa gaaggcactt attgatctat ccagtcaata ggtacgggag aggtacatga
     3661 aagtttggaa caaaataaaa caaaatgaaa atctgatgcg ctttattgag acgactcaaa
     3721 agcgcatcac tgattcagaa atgagtacga cctctgtcgt cgttgcttat tatttactct
     3781 tgtctttatt tccattgatt attgcttttg gtaatatttt accgtttctc cacatcgatc
     3841 aagagactgt actgacctat ttacgagaag tgattcctga gaccgtttac cagtttatcg
     3901 gtcctgcaat caaagacctt ttgacacaaa gttcaggagg cttgttatcc gtttctgcat
     3961 tagcggcttt atggtctgca agtcaaagta tcaatgcttt gcaaattgcg atgaataaag
     4021 cgtatggcgt agaaaataga aaaaattttt tgatcgtccg tttcttctca ctaattgtca
     4081 ttgtcttgtt catgattgct attagtgggg taactatcgt tcttgggttg ggacagctga
     4141 ttttggatgc tattcagcca atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa
     4201 ctttgaagtg gccaatcaca ttggtagctt tatttgtcat catgttcttg atttatttaa
     4261 ttgtccctaa tgctcagttg aagttgaaga cgatcattcc gggcacgatc ttcgctactg
     4321 tcggctggat gctgttatca caagtgttta gtatctatgc taggtatttt gcaacgagga
     4381 tcagcggtta tcagatcata ggaagtttta ttgtactcat gctttggctt aattttgctg
     4441 ctacgatcat cattttgggc ggaatcatca atgcagttgt tcaagaatac gtgacgggtc
     4501 atcaaatcaa aaaacgtcga gatccaagcc gaaaatggtt caataaattg aaaactaaat
     4561 tttctcataa aaaaaagtag aaacaagagt tcttattgta aatttatatc agtaatttat
     4621 ttgaaatttt atatctaatt ttcaattatc cgcataaaca ctatacattc aagcattttg
     4681 aagtatagaa aaacactcat cttaccacga atttaccacg attgaatgag ccttgatatg
     4741 accgtaaaac tactaaggga gatagcacga aactgtgtat ctcccgattt ttttatacag
     4801 ttgattactt cactccgatt tcaaatttca atattgcttt catcatgtct gtatgaattt
     4861 ctccttttag ttcttcatca acctgcataa tgattttacc actttcagag tggtaaaaag
     4921 gacgcaagca taatttagaa atataaccgt cataaaactt caaaatctct ttaactgcca
     4981 tttcatctcc gcttgacgct ttgcaaatca attcaaaaga gggatagcca taatctttac
     5041 tcatttcttt acttcctttc gctcaaaagc atttttaact tccttagtcc tctagttctt
     5101 ctactgcaaa tagcagaacg tgatacatgg tacagttctg aaatttctct gtcgttcatt
     5161 ccttgaaaat aacgtaataa aatcgcacta cgctgttttt ctgacagtct atgtaaaact
     5221 gtaccaagtg tttcatcaga tacataaatt gtaaagtgca agactttaaa tgatacacaa
     5281 tcataaaaac aggtatcaat cgttccacgt tcaacttgtt tcgtttcggc tatttcacaa
     5341 aacaatactt cacgtttaga atgtcttgca agctgtctgt ttcgactttt cactttgttc
     5401 ttaatgacaa tcagcattaa agaattgaac tgtaatctga cgatatgctc gaaagaagat
     5461 gtcctcataa tctcaccccc tttctgctaa tgctgaaata tataaacgga ataccgttta
     5521 taccctttag aaatacagaa aaatggtaag aaaaaagccc acacaaaaca tatatttgtg
     5581 cgagccttaa atactgcaat attactcttt cttttataac actatccttt tagcagattt
     5641 caaattttct tttatatatt gtttttagcc taacatacta tttaacccct tgtattatat
     5701 ctctaacttt tgaaaaattc tttttgaata tattgaaatc atgattgaag caatagaagc
     5761 tacaaacata acaacccatg tttcttgtat aaatacatcg aataaaatac cgtaaaccat
     5821 ttgtccaata ggttgaccgc acatagcaac tgctaccagc gtagccatta tctttcctac
     5881 taattgcggt ggtgtttgtg tctgaacaag agtgtaaatt tgaacaacaa acatagtaga
     5941 tacacccata gccacaaagc acatcactgt aactacccag taggaaacta ttgcaggcaa
     6001 ttctaaaaat agcgaaattc ccataagtaa aacagaaaca gaacaaataa gtaacaaggt
     6061 ataaaggttt tttagtttta actttgaagc aacaagagct gttaaaatac caccacataa
     6121 accacctaat gataatgctg attgtgaaaa accatatagt gtatctggca tttttaaaac
     6181 ttcagaaatt aagataggca gaccaacaat taaaacagca cttaaaacca aattgaaaat
     6241 gcaaaccaaa aagattacgg atataaaaat gggtttctcc tttttcaaaa aggtataact
     6301 atcttttaaa tcgtttttag caactgcaat tacacctact tcacgaggtc ttttttggtg
     6361 tggaatatgg ataaaaattt ccataatggc tgaaaaagta aaacatataa tactaacaat
     6421 taaaatggga ttgataccat aaaattgaaa aactactccg cttatagctg gtccgataaa
     6481 gtttgctaaa gtatttactt gatttattat agcattaccg acggtaagtt tatctgatga
     6541 taccaataga ggaatacttg cttgtaccgc tggttgatat gcaccagaaa taccataaag
     6601 taacatcaaa gcaacaataa acaaaggtat gataggaatt tttcccaatg acaaataaaa
     6661 tactaaaatt atgattgctg ttacaaaatc tagtatgacc ataatgtttc ttttatttgt
     6721 cctgtcagca ataataccac ctaccagcga caaaataacc ataggtaaaa aagagcaggc
     6781 tgtagcaata ccaaacaaag ctgatgaacc agtttctttt aacaaatagt acggtaatgc
     6841 aaatcgtagg atagcgtttc caaataaaga aattatctgt cctataacaa caagtataaa
     6901 atcacgatta aaaagttttt tttctgcttg cataaaatct ctccttttta tttaatcaaa
     6961 cagaccgtcg gtttatataa tggtttaaat aaactgctct tttatagagc agtctatcgt
     7021 atagggataa caaatacagt tatttacaaa actgtataaa tgcagaaatt gtttcttcgt
     7081 ctaataatgt gtctatgcct attccattta aaagggcatt gaatgtattg caacgttttt
     7141 ccataccctc tttatccgtc atttcaacta atgggttgag ccatacatta cttaatacca
     7201 tgataacttc tgcaatttca tgtgaatttt caatctccaa agacttatcc ttgatacctt
     7261 gttctaaaac gggttcaaca aattcaggta ctacaatttc atacatttgt tctatttgca
     7321 tagcaagaaa acgaggattg tttaatagtt gaggagaaac tgtcaataaa acgctttggt
     7381 tagaattgaa taccgctgtc ttaaatattt ctcttaactt ttctaaaccg tttaaactat
     7441 cgttatctct aattttagca aatattactg cattttcatt tccaattctt tgaaaaattg
     7501 cttttaaaat atcttccttt gaatcgaaat gatgataaat agcaccttta gaaagttttg
     7561 tttcattgat aatatcttgc aatgatgtgt tatcatatcc tttttcacaa aacatttttg
     7621 tagaaacatc taaaatgagt tttactgttt cttctggata tttatttcgt gccataactt
     7681 acctccaata caaaccgttg gtctgattaa agtatatctt agtattttat gcttgtcaat
     7741 ataaagactt gcaattttat aaaaatgcag aatgcaagcc tttttcattt attctacaat
     7801 cttccagttg ctttccttat gcggtgtcaa ttcatactgt gatacctgcg ttgcctttgc
     7861 ctgatttacc acaaatcaga ttttatccct ttcttcctct tgattgccca caacagaaaa
     7921 gaaaaagccc tgtcaagagc tttgccacca ttggtggtgc tttgcactct tgatcgggct
     7981 ttttgtttgc tgtatcttct cgcaagagga aaaaagttac tcgtcctcat catcaaaatt
     8041 gttattcctt aatacttctc gtaacagttc catatcttct tttgagttcg ggtttatcat
     8101 tttcacgact tgataaggtg tcttatactt atgtccctct atgctttccc caaacatatc
     8161 catactgtat aaatcatcat agcggtctaa taatttttga aacttcaaat gctggataaa
     8221 ttctttgatt gggtaaaggt cggacgcttc aatgcttttc ccgttgatat agtctgtaat
     8281 atattcccgt aacttttcta actcatattc cagccattct tcctcgctgt caaagaagtt
     8341 atcatagtgt ccatgtttca gttctgcatt tagacgttct tcggctctta aaaactgaca
     8401 gacttccttt tcagattgat tgacatactt ccattctccc gacaacaatt cattgggcgt
     8461 aacgtccagc acttccatta tcttttccag cgtttcatag gcaggatagt tcaaccctct
     8521 ttcaatcttg gaaaggctct gcatattgat accgattttg tccgcaagtt cctgttgttt
     8581 cattccccta aattttctta tggtctgtat attatttcct aaatggctta ttttcattgc
     8641 ataaccctcc atatctcgtt atatcttcgc tattataagt ctatcatgct taatatgatt
     8701 tgtaaaggca aaaaaagata ttgacaaata agcatataag aattactatg ttcttgtaat
     8761 cgaataagca caatagaatg aaatataaaa acattcaaaa agaaatacct atctgattaa
     8821 atccgtagat atgaatatcg gttgaaatgg aacttgttag aaaaggcaaa acagaacttg
     8881 ctgacaaagt atttctgaca cggtggcgga agcctctcag gcttaccgcc atgacacctt
     8941 gtgtcaaggg gaagaaatgc tttgtcggct ggcgagcctg cgagccttgt ctgtgccccc
     9001 gttatctaac aggggggcac tatttcacaa aagacaagtc aaaaaccata cacccccaaa
     9061 cctgtgagta gtaatgagag gttttaagaa gatagtacat atcacacttt ttgaaaggtg
     9121 gtttacaggt atttcaaggc tattgtggga ttggaggaat acaatgaaca atttagaatt
     9181 tgcacttgaa atgaagaaca aacgtcttgc cagcggtctt tcacaaggag aacttgcaag
     9241 tcttacccat gtatcaagat atagtatcaa ccgctttgag aacggaaaag ctaacgccag
     9301 caaggaaaca cagaacatta tcctgcgttg tctgaattac tatatctgcg ataagccttt
     9361 ttatctgctc gttgattatc tgtctgtccg ttttccaacg actgacgcat tggaagttat
     9421 ccgaaaggtg cttggcatga aagcagatta ttttatccat tatgactatg ggtattatgg
     9481 ctataaggag cattatgctt acggggaaat caaggtcatg gcttctgatg atgaacacat
     9541 gggcgtattt ttagaattaa aaggggcagg ctcacgcaac atggaatatg tcttacaggc
     9601 acagaacagg gactggtatt cattcttaaa ccgctgtctt gactgtggcg gtgttatccg
     9661 acgttttgac ttggcaatca atgatatgtg cggtctgctg gatattcctg tgctatctga
     9721 aaaatacaga aatggtggtg cggaatgtcg ctgtaaaaac tatgaaaatg tacaaggtgg
     9781 aaaattaagc ggaaaaaacc gtaatttagc aagtaccctt tatatcggct caaaggcaag
     9841 cacaaaatat ttctgcctgt atgaaaaaca aaaggaacag gcaacgaaaa agaaacatac
     9901 cgatattatc aaccgttttg aaattcgtct gcgtgacaaa aaggcggtac aggcagttga
     9961 agaactgtta ttgacgtata accctcatgg gctggtgttt tatctcatta ccgattttgt
    10021 ggaatttcct gactatccat tgtgggagat attcatttcc catgacagtt taccttttga
    10081 aatgaaccct gtacctgtca atatggaacg cactctgcaa tggctggaaa gacaggtcat
    10141 gccgtcaatc gtgatgatag aggaaatcga caggctgaca ggttcaaact acatgaaaat
    10201 gattgatgaa tgtacccacc tttctgaaaa acaggaaatg cttgtggaac agatgtgtac
    10261 ggacatatca gacgtaatcg aaagtgaggg ggtgttttat gagtaataca caggatattc
    10321 ccgtatggga aaaatacacc cttaccattg aagaagcgtc aaagtatttc cgcattggag
    10381 aaaacaagtt aagacgattg gcagaggaaa acaaggacgc tggctggctc attatgaatg
    10441 gcaaccgcat acagattaaa cgccgacagt ttgaaaaggt tattgataaa ttggacgcaa
    10501 tctaatgcaa atgagccttg tatgtgttat gatgaacaca agtcatatca aggctctttc
    10561 caacaaggaa aggagcagac accatgaaag aaaaaagacg ggataacaaa ggtcgtatcc
    10621 tgcatactgg agagagccaa cgaacagacg gaaaatactt atataaatat gtagacgcat
    10681 tgggaaacac aaaatatgtg tatgcttgga gattgacacc cacagacccg acaccaaagg
    10741 gaaaacggga aaaaccctca cttcgagaac tggaacagca gataagacgg gatattgagg
    10801 acggtatcga cagcacaggc aagaaaatga cgctttgcca actctatgcc aaacagaacg
    10861 cacagaggac aaatgtgaag aaaagcacac agaaacaacg ggaacaactc atgcggttat
    10921 taaaagagga caagttaggt gctaggagca ttgatacgat aaaaccctct gacgctaaag
    10981 aatgggcgtt acgcatgaaa gacaaaggct tttcctacaa taccattaac aaccataaac
    11041 gctcgttaaa agcgtcattc tacatagcca tacaagatga ttatgtgagg aaaaaccctt
    11101 ttgatttcaa gctaagtgaa gtcatagaaa atgataccaa agagaaagtc gcattgacag
    11161 aggaacagga gcaagcctta ttgtcattta ttaagacgga caacgtgtat cacaagcatt
    11221 atgatgatgt gctgatactg ttaaagacag gacttcgtat ctcggaactg tgcggactga
    11281 cagtagccga cattgatttc aagaatgaag ttgtgattat cgaccaccag ttactaaaga
    11341 gtaaggaaca gggctattat attgaaacgc ctaagacgaa aagcgggata aggcaagtgc
    11401 cattaagcaa agaaacgata caggcatttc aacgggttat caacagccga ccaaagataa
    11461 agtcgataga ggtagacgga cacagcggtt ttgtgtttat caatccgaaa ggtaatccaa
    11521 aagttgccat tgattataac gccttattta tccgtatggt aaagaaatac aacaagcacc
    11581 acaaggacaa tcccttgcca catattacac cgcatacgct acgccataca ttctgcacaa
    11641 ggctggcaag caagaacatg aacccgaaag atttacagta tatcatgggg cattcaaaca
    11701 tcagtatcac aatgaactgg tacgctcatg cgtccataga taccgcaaaa tcagaggttc
    11761 agcgtctaat cgcatagaag tatttaccac gattttaacc acgattgaca gggaaaatat
    11821 aagaagatag acctagatat gcgaggttta ccacaaaagc aaaatgcccg tagagccgat
    11881 aaaataaggc tttgcggaca tttgagaaga tataaaaaga tag
//
LOCUS       DACCYH010000091        11851 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.095, whole genome shotgun sequence.
ACCESSION   DACCYH010000091 DACCYH010000000
VERSION     DACCYH010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.095"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            5..2377
                     /locus_tag="IVY85_002346"
     CDS             5..2377
                     /locus_tag="IVY85_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045346.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..3898
                     /locus_tag="IVY85_002347"
     CDS             2438..3898
                     /locus_tag="IVY85_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP8045347.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3909..6113
                     /gene="topB"
                     /locus_tag="IVY85_002348"
     CDS             3909..6113
                     /gene="topB"
                     /locus_tag="IVY85_002348"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8045348.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6353..6946
                     /locus_tag="IVY85_002349"
     CDS             6353..6946
                     /locus_tag="IVY85_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP8045349.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6959..7858
                     /locus_tag="IVY85_002350"
     CDS             6959..7858
                     /locus_tag="IVY85_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP8045350.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7861..8358
                     /gene="ssb"
                     /locus_tag="IVY85_002351"
     CDS             7861..8358
                     /gene="ssb"
                     /locus_tag="IVY85_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8045351.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8724..8984
                     /locus_tag="IVY85_002352"
     CDS             8724..8984
                     /locus_tag="IVY85_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045352.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9429..9635
                     /locus_tag="IVY85_002353"
     CDS             9429..9635
                     /locus_tag="IVY85_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP8045353.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9683..9886
                     /locus_tag="IVY85_002354"
     CDS             9683..9886
                     /locus_tag="IVY85_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045354.1"
                     /translation="MVDKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFEDLVKIAG
                     YELHYQENMETYENEYWLTKLEN"
     gene            9902..10339
                     /locus_tag="IVY85_002355"
     CDS             9902..10339
                     /locus_tag="IVY85_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045355.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10332..11039
                     /locus_tag="IVY85_002356"
     CDS             10332..11039
                     /locus_tag="IVY85_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045356.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11419..11598
                     /locus_tag="IVY85_002357"
     CDS             11419..11598
                     /locus_tag="IVY85_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045357.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
BASE COUNT         4435 a         1967 c         2464 g         2985 t
ORIGIN      
        1 gaacatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 actagtacga ttgcaaaatg aattaaacga acaaattgaa aagatgtttg aacaccaaaa
      121 aatgacgaac ggtcaaccaa tgaacgacaa gaggaatggc cgttcatggt ttaaccagca
      181 agaacgaatc gaaaataaag tccaaagtct gagagaagag atcaagcaac aagaagaata
      241 cgtggaaaag cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgata atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg gctttttcag ccgccacgat ccgaaaatac caacggaagt tggaaacatt
      421 agaacagctg aaagaaaaat cagaaaaagg aaaagaaaat atcctccctg aagtacaagc
      481 gatcattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 aggctatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctag
      661 accaactgat tctatgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tcgaattgat
      781 cgatcaactc aaaacattgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaggaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccaa tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaaacaaga
     1081 agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtcaaa gaacttttga actttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaaggg ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actatacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cgtcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 tgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctttagaaac gccagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtaa ttttaaaagg
     2221 aaaaagttat ggaaaagaga tccaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg agacagtgtc agaagtggca aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg tgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg ttttaaaatg accgagaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagatt atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ccctaagcaa caatttaaag cagccactga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtggaa aatcttcatt ttgaaacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gcagaaaaag aagggttgat
     2881 tgccgaacta aagccaaaat atgaacggca aaatttctta gttcgagacg atcgtttaga
     2941 tcatgcggtc gcctttttat ggaaagatcc acagacaaaa gagacagtag gggcgagtta
     3001 tcaaggaacg ttcattgatt atgaacgttt tggtgagaga ggcacatata agcatataga
     3061 taaaaattcg acagcgaatc atggctttaa cctaaagatc ggtgacccta agcagataaa
     3121 attttttgag agtagtattg atttacttag ctatgctgca ctgaatcgtg atcagttaaa
     3181 cgatacgtgg ttagtctcta tggaagggtt aaaacaccat gtgatcagtc attattttgg
     3241 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatccatcg agatttgtgt
     3301 ggataatgac cgtgcaggac atattttcta cgaaaaagaa caattaatgg gcgcagtcga
     3361 tccatttacg aatcagaaag tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc
     3421 aaaagaatac aaaatcattt atgaagaagt cgcaaaagaa gaaaaggtca caccagaagc
     3481 tattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaac tggtttccgc
     3541 tcacaaagta aatgcgtttt ttggccagca gctttctgtt aacgattcaa tagaagccat
     3601 taatttgaaa gatatttgta gagaagtcgc aaaagaatta aaagtttgtg aacgcgtgga
     3661 tggtacgtac gattttgatc gtttttatca agaaaaaggg gatattaatg cgcagattct
     3721 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttgt
     3781 acctgaagta aaaaaggact ggaacgatca attgaagcat gagatccagc aacaagaaat
     3841 cagaaagcaa aaacgtgcca tgttgtttca acaaggcaga caacaagaaa gggagtgaaa
     3901 tcgtcatgat gaaaaccgtt attttagcag aaaagccgtc acaagcgaaa gcttatgccg
     3961 atagcttctc taaagccact cgaaaagacg ggtattttga aatccaagat cgattgtttc
     4021 ctggtgaaac tgtgatcacg tatggttttg gtcatttagt cgaattagat tcgcccgata
     4081 tgtacgatga aaattggaaa caatggtcat tagaacattt gcctattttc cctacacatt
     4141 atcactatca tgttcctaaa gataagaaaa agcaattcaa ggtggtgaaa cagcaacttc
     4201 aatcggccga caccattatt attgcgacag atagcgatcg tgaaggggaa ctgatcgctt
     4261 ggacgatcat tcaacaagta ggcgccgatc aaggaaaaac ctttaaaaga ctttggatca
     4321 actccttaga aaaagaagcc atttatcagg gattccagca gttacgagac gcaaaagaga
     4381 cctatccaaa atttgaagaa gcacaagcac gtcagatcgc cgattggtta attgggatga
     4441 acggtagccc actgtatagt ttactcttgc agcaaaaagg cataccagga agcttttcct
     4501 taggcagagt ccaaacccca acgctttaca tgatttatca attgcaggaa aagattagaa
     4561 atttcaagaa agaaccttat tttgaaggaa aatccgaagt aatcgctcaa aatggcgcat
     4621 ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca agaagcattt gaagattatt
     4681 taaaagaaaa gggggttcaa ttgggaaaac agccaggcat gattcgccaa gttgaaaccg
     4741 agaaaaaaag cgcggcaagt ccacgtcttt tttctctatc cagccttcag tctaaaatga
     4801 atcagctcat gaaagctagt gccaaagaca ctttggaagc catgcaaggc ttgtatgaag
     4861 ggaaatatct aagctatccg cgaacggata ccccttacat tacggaaggg gaatatgcgt
     4921 atctgttgga tcacttagac gaatacaagc attttttgag ggctgaagaa atttcgactc
     4981 ctattcatac accgaatagt aggtacgtga acaacaagaa agtccaggaa cattacgcaa
     5041 tcattcctac aaaaacagtt atgacagaag cgacgtatga aagtctgaca cctttacaac
     5101 aagcgattta cgagcaagtt ttaaaaacga cagtggcgat gtttgcggaa aaatatacct
     5161 atgaagaaac aacgatcttg acgcaagtcc agcagcttca gttaaaggca atcggaaaag
     5221 taccgataga tctaggttgg aagaagttat ttggcaaaga gagcgacgga aaagagaagg
     5281 aagaagagcc gctacttcct aaagtgacaa aaggagaaat ggtgacggta gatctacaag
     5341 tccttgaaaa agaaaccaag ccaccacaac cttataccga aggtacgttg atcactgcga
     5401 tgaaaacagc tgggaaaacc gtggatagtg aagaagcgca atccatattg aaagaagtgg
     5461 aaggaatcgg tacagaagcg acacgggcga atattattga aaccttgaaa caaaaggaat
     5521 atatcaaggt agagaaaaat aagcttgtgg tgacgaataa aggggttttg ctttgccaag
     5581 ctgtggaaaa agagccgctg cttacgagtg ccgaaatgac cgccaaatgg gaaagctact
     5641 tgttaaagat tggagaacga aaaggaacac aaaccacttt tctagccaat atccaaaagt
     5701 ttgtctctca cttattggaa gtcgtcccag gacaaatcca atccattgat tttggatcga
     5761 cgttacaaga agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat ttaggaattt
     5821 gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa ggtggcagct tgtacgtctg
     5881 aagcctgtga ttttaaatta tggacaacga ttgccaaaaa gaaactcaca gcgacacagc
     5941 tgaaggagat tattcaaaat agccgaacaa gccaaccagt gaaagggtta aaaggtcaga
     6001 agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc tttgaatttt
     6061 ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cacgacaaaa taatggaggg
     6121 ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa aaagccaaaa
     6181 ttcgttcaga acaagagaaa ttacgtgcca gtaaaagaag acttcagcaa ttaaagaacc
     6241 agcaacggat tcaaaaagaa atcgaattag atcgatagac attaaccttg ttacaaatac
     6301 actatcatga tatatttaat gtagtgtatt tgtaacaaaa ggggggaagt aaatgcaaga
     6361 gaaactgaaa gaattgttgg ctattcgcga tggaaattta tctatgaagg aagccagaaa
     6421 attaggaatc gcagcaacca ctgtacaaag attggtgaat caaggaaaat tgatcaaagt
     6481 tgatcgtggt tattatgtag aggatggtca tggaatagat gatttatttt ggcttcagca
     6541 acgtttttcc aggggaatct tctctcatga aacgacttta gatatttacc agctttctac
     6601 gaatatgccg aaattcattc acttaacttt tccccatgga tataacgttg atcgttcaat
     6661 tacaaaagag cagcagttac aatttcattt cgtgaagaaa gaagtttatg aactaggaaa
     6721 agtagaggga acttcctttc aaggaaatcc tattactatg tatgataaag agcgtacact
     6781 ttgtgatatt tggaatccga gatatgaaat tgaatatgaa atgaaattag aagcattaaa
     6841 agagtacatg gaagacccat tgagagatcc aagtaaatta agagattaca tgacaaaact
     6901 acatgtagaa aaagaaatga aataccacat gcaaagctta tattgatgga ggaaaaagat
     6961 gacaccgaca caatttgcag caaaaacaag aaatttagca aagaggacag gattaaatgc
     7021 acagttagta caacggcatt ttatgatgga tcgtttaatt gaacaaattt ctgaatctaa
     7081 gtataaaaat gatttcattt tgaaaggtgg ctttttatta ggatcaaaat atggaatcga
     7141 tagaaggacg acaattgata tcgacacgac atttagaaat agcaaattaa ctgaagaaaa
     7201 attgaagagt atttttaatg atttaactag gaaacccacg aaagaaggta ttgttttctc
     7261 tattcaagga atgaaggaaa caagagaagc cgatttttat cctggatttc aaattaaatt
     7321 ggtggctaat ttagagaaag tacgtgtgcc atttaaaatg gacgtgacaa caggagattc
     7381 tatttatcct gaagtagata cacactatca ccaattaatg tttgaagata aaaaggtgga
     7441 gattccagct tatccaacag cacaaattat tgcagaaaag ctaagtgcta cgttcagctt
     7501 tggcacagat aattcgagag caaaagatta ctacgactta tttaccattc cgaaactaga
     7561 aaatatcgat acgaaagagt tatacaaaag tgttcgaaat acatttacaa aacgtgggga
     7621 tacaaaaccg ttgtcctttt attatgaaag agaaatggat aagatcatag gaaatgacta
     7681 tttggaaaag cagtgggaaa aatatcgctc agttaacacg tttgcgaatt ccatttcatt
     7741 tacagataca gtcaacgaaa ttgatcacct aatgaaaagt gtgatcaaaa ttgaaaatca
     7801 agaaagaaaa cggcagttta aaagatcaca acaaatgaat caagaaatgg agagataaag
     7861 atgatcaata atgtaacttt agtaggacgc ttaaccaaag atccagattt acgttatacc
     7921 gcgagtggta ctgcagtagc gacttttacc ctagctgtca accgaaattt cacgaatcaa
     7981 aacggcaatc gagaagctga ttttattaac tgtgtgattt ggcgcaaacc agcagaaaca
     8041 atggtgacat tagctaaaaa aggtagcttg attggtgtag ttgggcggat tcaaacgcgg
     8101 acttatgaca accagcaagg acaacgtgtg tatgtgacag aagttgtggc cgataatttc
     8161 caactattag aaagcaaagc agcgacagaa agccgtgcgc acgctgatca atcgtccacc
     8221 agtccaagca ccaccacttt tgaacaacgg gatacagcga cgcctaataa caatggattg
     8281 aacgctttac agaatccatt tgggaatcca tttggtggac aatcaatcga tattagcgat
     8341 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     8401 aatttagtca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     8461 tggttttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     8521 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     8581 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     8641 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     8701 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     8761 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     8821 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     8881 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     8941 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaaaaaag aagagttgca
     9001 atcctaatgt aggactgcag ctctttttgt tttacataga aacaagaaaa atgaaaaggc
     9061 ctttttagga aggcgcgagc gccttctcaa ttgaaaaacc tctctcactc caaccgccac
     9121 cgccgttcga gaagaaagga agtgaacgtt gcacgttcac taagataaag ggtcttgcaa
     9181 agcaagacaa aatagaaaaa acttcctctc accaaaaggt tcgttcctgt tcgttatctc
     9241 tttattgatt tgccagaccg gcaacaagtt gccttttatc accaaaaagc gacaaggaca
     9301 agggtaaatg gcacgctgcg cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc
     9361 aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa cctttcctaa gaggaggcag
     9421 aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa ttattcccaa
     9481 actatcatgc accacatact gtcgaatgcg attgtggaca aagagcgaaa tgtatcaaaa
     9541 gctatcgttt gtatcaatgt ccaacatgcg gaaaaaaata caccttgtct tatggcgatt
     9601 atattttgat tgatgagaaa ggaaagaaac gctgatgaaa acataacata aattattgac
     9661 tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc atttctgaaa aagtagcaga
     9721 agctttactt attccgcggt actggacgat agaaacccct ttagtagagg atgcaccgac
     9781 tatgctattt gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaacat
     9841 ggaaacttat gaaaacgaat attggttaac caaattagag aactaaaaag gagaaaaaaa
     9901 tatggctcaa aattactatg acgaatttgt caaattacct ttggataaaa tggcacaaaa
     9961 aatggaggat atgacattcc tataccatga gacacgtgtt ccaaaaaaac attataagga
    10021 aagattgtcg gtagctgtgg aagaaatgat tgaatcaggt gtcgaaatga atctcattgc
    10081 gacgtactat cgaacactgg aagaactgaa aaaacaaaat gcaaaatggt ttttccaagc
    10141 cttactatgc ttggaagtag gtgtcaaacc aagcactatt aaaccttctg aatatcaagc
    10201 tttagaacta acgtatggga aatttgtcga aacgaaaaaa gccaagactg tttcaagtga
    10261 atggttagat tattttgaaa atatcaataa gtatggcgcc tattacacca tgaaaaagga
    10321 ggacaacgaa aatgaataag gtgatttatc aactaacaac ggaagatgtg tcacactttt
    10381 taacaaacaa agaacatttg aatgaagtaa tcagacggat tcaaaaagac tttagttgta
    10441 tggaagaaat gaagtacttt attcaaagag tcgaagaaca attgtatttg gaaagtgaaa
    10501 cgagtcaaac gaaggtcaca gtcagcaata attatttaga cgtgtataaa catcgtcctg
    10561 aaacggtaaa aaaaggaacg gagcttctac gactagaaac agaaaaatac atcatgaatg
    10621 tttacctaga aaggagtgaa ctggtcgaaa actttgtcag ctgcacgatt tgcaggaaac
    10681 actattactc agaatcagaa tattattcaa tttttgagag catggaatta atggaagaag
    10741 aaatggaaga gctaaacagc aaaaatgcga cgatagacga ttattatctt gtatttacta
    10801 gatttttgac gtgtggaacg gaatctatcg tgttaatggg aatggattat gaactcgctg
    10861 tacccgaagc atttttgagg gaatatgtag aagaagtaga taacccaggc gacgactggt
    10921 acacgacttt aactgatttt ttagatacct acacttacga cgatacagaa gaaatccaaa
    10981 atgaacttga ccgtaaacga atgccatatc aacgatttga aaggaaaaaa aggcaataat
    11041 catttatacc gaaaagtgag aatgtgtgaa ataagaacaa aaaccttgaa attaagggaa
    11101 tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa
    11161 gcgggcaaag cccgataacc taagagaaag aaggagatcc atatgaataa aatcattcct
    11221 ctgttgttcg tactgtgtat tggcgagata cttatgcgtc atggtgcagc tgtacattta
    11281 aacttcgtag tattcttagg aatactagta ttttgtttga ttttttggcg ctttgtgtta
    11341 gcccttgtga gtctactatt caagttgatt gcagccatat ttttcatagg gttatttcga
    11401 tgaaaggaga gagtactaat gccaattgca ataggaaata aacggttacc cgtgacatta
    11461 gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtacggaaa aagtgaatct
    11521 aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg atttgatata
    11581 ccagcactaa aaaagtgacg tcacttttca tttgaagaaa caaaagtgat agcacttaat
    11641 tgttcctact atttagaata ttttctatgt cctttttatt ccattttgta tcaataacta
    11701 tgaaatcttt aaagtatcca gatcaatttt tttcttacct gattctaaag attttgtttt
    11761 cttaaacata ttatgatttc ctttcgacta ttttcatata aatgttaaca tagtttatgg
    11821 attcatggaa tgttaatcta atttaataga a
//
LOCUS       DACCYH010000092        11767 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.096, whole genome shotgun sequence.
ACCESSION   DACCYH010000092 DACCYH010000000
VERSION     DACCYH010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.096"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(158..535)
                     /locus_tag="IVY85_002358"
     CDS             complement(158..535)
                     /locus_tag="IVY85_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045358.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(835..1716)
                     /gene="galU"
                     /locus_tag="IVY85_002359"
     CDS             complement(835..1716)
                     /gene="galU"
                     /locus_tag="IVY85_002359"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8045359.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1759..2778)
                     /locus_tag="IVY85_002360"
     CDS             complement(1759..2778)
                     /locus_tag="IVY85_002360"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8045360.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(3411..4415)
                     /locus_tag="IVY85_002361"
     CDS             complement(3411..4415)
                     /locus_tag="IVY85_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8045361.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(4428..5477)
                     /locus_tag="IVY85_002362"
     CDS             complement(4428..5477)
                     /locus_tag="IVY85_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8045362.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(5494..6315)
                     /locus_tag="IVY85_002363"
     CDS             complement(5494..6315)
                     /locus_tag="IVY85_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8045363.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(6302..7081)
                     /locus_tag="IVY85_002364"
     CDS             complement(6302..7081)
                     /locus_tag="IVY85_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8045364.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(7098..7568)
                     /locus_tag="IVY85_002365"
     CDS             complement(7098..7568)
                     /locus_tag="IVY85_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8045365.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7580..7999)
                     /locus_tag="IVY85_002366"
     CDS             complement(7580..7999)
                     /locus_tag="IVY85_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP8045366.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(8080..10848)
                     /locus_tag="IVY85_002367"
     CDS             complement(8080..10848)
                     /locus_tag="IVY85_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8045367.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            11048..11272
                     /locus_tag="IVY85_002368"
     CDS             11048..11272
                     /locus_tag="IVY85_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045368.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3411 a         2479 c         1908 g         3969 t
ORIGIN      
        1 ataaaaaaag gaatggctta tcccgataca acaagcattc ctttaaaaaa gattctttta
       61 tataagtgaa aaaagctttt ttccaccaat taaataaatc tctcatctca atagtttatc
      121 cattatttta tctttttttg cgtataccta ctaaatatta ccgtcttctt ctcgttcttc
      181 tactttgttc gagagaatct gtactagaaa aagtagttgt atgtctacct tttctgccta
      241 tccgagactt tcttttcata tatgggatat aaatgattag atacagtaca gcaaaaaata
      301 aagcaaatac gcaagcttcc caactaccta acgccaattc aaccatcaag gtagcaaccc
      361 caacggatgc tgcataccag ataaaaatca gaaacgtttc tttttttctg accatgcgaa
      421 acatgataaa tagactaata agcaatagtg gcaacacaaa tatccaattg cttccttcca
      481 tcagccactt gagtatatca agcagcgact gtgcaacagc gactaatttt cccatagtga
      541 acacacacct ttttttataa ttaaaaaatt cgccttttta ttctactatg aatcttctgg
      601 cttggctttc atttctctta tgtaatcatt ttcttttata caacttaaaa aaaaattaaa
      661 ccattagcat aatattttct gaatctattc acgaaagtct ttttctatct ttcgttttcc
      721 acgactcaac tgatctagag tcaaaaaact aaaacgacag ttgcgcaaat gacgcacaag
      781 ctgccgcttt aataacaaat agcctttcat tctcagcagg ccacttattt ccagttaact
      841 ttctgttctt agctttttac tcaactggat aatatatgca cttaatgaag tcccaatttc
      901 cgaatgttga agtccatatt caatatttgt cttcatatat cctaacttat cgcctacgtc
      961 gtatcttttt cctttaaact ctctcgcata gaccgcctct gtctggttaa gagtctgaag
     1021 tgcatccgtt agctgtatct cgtttcctgc tcctggttct tgagtttcta atatctcaaa
     1081 tattttagga gtcagtagat aacgtccgat aatggctaaa ttacttggtg cttttcccat
     1141 ttcaggtttt tctacaaacc cttttactct gcaacgaccc ttttcgctat ctgttttagg
     1201 atcaataatt ccgtatttag atgtttcatt ttctggtaca cgcatgacgg caacagcgga
     1261 agcttgtgtt tcttcataat ccatcatcaa ttgttgagtc aatgggatat catctttcat
     1321 caaatcatcc cccagcatga cgacaaacgg ttcatttcct ataaatgctt ttgcttgaag
     1381 cacggcatcc cccagtcctc ttggatgaga ttgacggatg aagtgaagat tgacttgagt
     1441 cgtttcttca actaaagcga gtaattctgt tttttccttt tcccgcaaat tgttttccaa
     1501 ttcgatattt gcatcaaagt gatcttcaat gggtcttttt tcttttcccg ttacgatcaa
     1561 gatgtcttcg attcccgaat ccaatgcttc ttccacaata aactggatcg ttggtttatc
     1621 cacgattgga agcatttctt ttgccatggc ttttgttgcc ggcaaaaaac gtgttcctag
     1681 cccagcggca ggaatgactg cttttttgac cttcacttgc tgttcctcct aaacgacgat
     1741 taactattga tcatctattt aacgaagaga aaattcattc tccatttttc cgtcacgtaa
     1801 catgatttct tttgctgctt ttttgatatc ttctccatta tacaaaacat tatagattgt
     1861 ttgagtaatt ggcatctcaa cacctagctg ttgagctagc tctactgctg cttttgtcgt
     1921 tgccacaccc tcaacgacca ttcccatgtt ttctagaact tcttccagct tatgtccctg
     1981 tcctaacaag ttaccggcac gccaattgcg agaatgaaca cttgtacaag taacgatcaa
     2041 gtcccccaca ccactcaaac cgataaatgt cagaggattg gcacccatcg cgacaccaag
     2101 acggctgatt tctgccaatc ctcgagtcat gattgctgct tttgcatcat ctccgaatcc
     2161 taatccatgg atagcgccgg caccaattgc aatgatattt ttcagtgctg caccagtttc
     2221 tacgccaatc acatcattat ttgtataaat cctgaaataa tcattcatga acagtttttg
     2281 tacataggct gctgcttttt gatcaatact tgcagctgta attgttgtga tatcatgtac
     2341 tgccacttcc tcagcatggc taggaccaga taatacaaca attgcctgtc ttttttcttc
     2401 tggaatttct tcagccaaaa cttctgagat tcgtttatgc gtatcttgtt ctagtccttt
     2461 acttgcatgt ataatgattg gcttagtatt cattttacca ataagttcct gtgcgactga
     2521 acgaatcgct tttgttggaa caacaaataa aatagcatct gcgttatcca ctgcttctgc
     2581 caattcttta tatccgataa tcgattctgg gatcgtcaaa tcaggtaaaa aatgctgatt
     2641 tgtatgatac ttattgattt catcaatctg ttcaggtaca tttccccaaa tacggacatc
     2701 atgcccgttt tctgccaatg tttgtgctaa agcagtgccc caagatccag gaccaagaac
     2761 tgcgattttt tgtttcatgg atattcctcc aaataaataa ttgataattt ccattatagt
     2821 atacatttct taaagttggg actaatttcc attgttttta agtgtttgtt tacttctttt
     2881 atcgttttta cttgaaataa tcaatgatat gttacaatag tctacatttt atttttacac
     2941 tcgttatgct ttgcttgttc ctaagaggaa gcagttttga caatgttact accagcatta
     3001 ctattaggag gttaggaaac ttctcctagt gagtattgat ttaaaataca taagagacag
     3061 attgaacatt tctaaatctg tcaggtcctg caacggacag attgtctctg ttgacggttg
     3121 acataggaac ttttgtttta gtcaacattc tggatacaaa acgctatgta aactggcgca
     3181 actcgttgta cttggacttt tactacggat ccatgcaaca actaaatagt actattgcat
     3241 aacaaaaaat ggttggcacc cacatgtccc attaaacaga ttataagttg aactgtagat
     3301 aatgcaaaaa atgtgtttta taacgtaatt gatgccaata ttttaaaaaa ataaagggag
     3361 cagttatcgc tgctctcttt attttttata ttctttttat ccagtctatc ctaataatca
     3421 aactgacgat agtagcggcg tatctctaat ggatggcagt tcaactgctc cacatgagca
     3481 tcgatgcgtt gtgtcactac atgcatcaat aagaaagaca aaacacgtcc gcgatatttt
     3541 tcactgatac cttctactgg atagtctttt gtgtctatca atgtataatt gctgcaaatt
     3601 tttggcaaaa gtttagccac acgttcagat aacactctct gttcatcttc gcctaaaaat
     3661 aatgtgactg gggttgtttc ttctacgatc tctaacgtcc cgtgaagaaa ctcggcagca
     3721 tggatagatt tcgatggaag ccagctttgt tcttcccagt aacacatggc atatgaataa
     3781 acagctcccc attgattccc tgctccaata aaataatgca tactatcacg tcgatgtttt
     3841 tctgcaaatg ataagccaaa tgcatctgct tttttctctg cttccactaa ccctgttggt
     3901 aaataacgtt cgagctgttc atagtattct gaataagctt cgaattctcc attcaaatac
     3961 atcaatcgat ctgctgccat aaaaaatttg atttgttctg ttccaggagc tggataagtg
     4021 accacaaaat cgcatagctg agacaactta ctgttcgctt tatcgataaa tccaatcagt
     4081 gtcgctccta ctttcttgat ttgttctact gctttcacta cttcttgagt tgtaccggta
     4141 acagaggaca aaataacgat agaatctttt gttaatcttt tgtttccagt agtgtaatat
     4201 tctgcagcat gctgtacaaa gacaggcaag ttgcttttcc cattcatata agtgacagct
     4261 tgcatcgagg aagcatacgt tccaccgatt cctagataat agacagcgtc aaatttttcc
     4321 gcataaagtt tatcgatgat ttcttctact tgcggacgta aagctagtgc gccttttatt
     4381 gctttgattt gttcttcttc attgaatttc agcatgattt ccacctctta taatttactt
     4441 ttcaagactg tatcaaagtc ttcaaaaatc tttttgttca agtcaatacc tttagctact
     4501 gcggttttat aagcgaatag ttgaaacgga acgacaagcg ctaatacaga cagatattca
     4561 tcacattcta ttgctaatcc aagtgttttc tcgtttcttg ctttttttgt ggtaatggtg
     4621 agagtctctc caacgtaccg actcatatat ctttgcaact tttgcgaacg ttccttattt
     4681 acactttccc cttcaataaa gaatagcaaa tgcgaagcat ctgcctccag gtatgggccg
     4741 tgcatataag cttctaactc aaatccttgt gacggaacgc ggattgtttc agtcaatttc
     4801 gtctctgctt ctttagctgt tccccagttt ggaccatatc caatggtgac gaaacgctgt
     4861 gctaaacgaa ataaactttg atatccatca aaaaattctt ccgttttttg aataatcgct
     4921 ggtaaatggt tgattatttt gcgcaactgt tgcatgtagt cttgttctat ttttttgctg
     4981 attttatttt tacttattcc aattcctatc cctaagagta gtaactgtaa aacagtcgca
     5041 gaatagcctt ttgtaacgaa gcctactgat tctaccccgg cacctaaatc aataatttgt
     5101 gcagcttcta aagcaagtgg actttgcacg tcatttgtga tagcaacaac aggtaaccct
     5161 tgtttttgta tcatttttac tgcttcaaca gtagaagcac ttttcccgct ttgtgacaca
     5221 gcaatcacag tatcgattga aggagaaagt tttccataat tgaaaaaata aaaaggttct
     5281 tgaatatcga cagtaagatc accataagat tccaatgctg gcttcgctgc taaacaagcg
     5341 ttataagatg aacccgttgc taagatcagt aaattcactg tttttttcat atgttctctt
     5401 gtcgaaaaat cattcttttt caagatcgta cttagtactt ccttttcttc atagatataa
     5461 tcttgcatcg taaacatttt ctctgctcct tttttaaaaa atccctatcc acgaaccaaa
     5521 aattccaaag aaagctagtc cgatcaagat cgttaatggt ttcacttctt tcttcaatag
     5581 ataaaacact atgccaaatg ctccaagaca taagatattt ggaacgatat catcaaaaat
     5641 actttgaaca gtcacagcat tttctcctga accgattttt aaaggaatat tgatggtgat
     5701 catcgtggca gtcattgctc caacgaccat cagtccaata atagacgcgc ctaatgtcaa
     5761 actttccatc ataccgtttg cctggatctt tttcaaaaaa cccgttccta agcgatatcc
     5821 ccaacctgtt gctagatagc gaaacagaat gtgcggaata ttgaaaatca aaatgaacaa
     5881 gatagggcct aaaatatttc cttgcatggc taaagaggta cccacacctg tggcaatcaa
     5941 tcgcaaagtt ccccaaaaga aagaatctcc taaccctgca agaggtccca tcaaagatgt
     6001 tttgatacta tcgatcgcag tcacatcaaa ttcaggatca tccttatttt cttcctccat
     6061 tgcagccgtg attcctaaaa tcagtgtaac gatatgtggg gtcgtgttaa aaaactctag
     6121 atgccgtttc aaagctgctg ccatttcttc tttcgactga tataactttt tcaaaatcgg
     6181 aatcattgca taaacataag ccatattcat ctgacgttca taattccaag aaaactccat
     6241 ttgaaaagaa cgccaaaata cgtgatttag ctcttttttc gttattttgc tgtttttgat
     6301 ctcagaattc ttcatcttct tctacctccc ctgcagttgt ttgaagtggt agttgacgga
     6361 cattcataat attgactacc acaactgcta aaatcgcacc aaaaatagct actccggtca
     6421 caggaatctc taagtaagaa gctatagcaa aacctaagaa aaaatatggt gcaacttgtt
     6481 tgttgatcag caaacgagca agcatcgcaa aaccaagagc tggaatcaac cctgtagcta
     6541 ctgacaaccc cttttgaacg aagtcaggaa tcatatttaa tagatttccg atcgtattgc
     6601 ttcccaccat ataagaaacc attaccacaa caccaagcat caaggaaaga ccgataccgg
     6661 caagcaagtg catatgctcg actcctttat aatttccttc ttctgcataa ttgtctgctt
     6721 tatggttcat aattggaatc aaaagcccta agtaaatatt tttcaatatc aatgtcaacg
     6781 tagcaatcgg caaacctagt aaaagcgcgg tttctgtacc ggctccagct gtaatagcaa
     6841 aagccgtccc taaaatccca ccggtcacaa catcaggtgg aatcgaagca cctactgaga
     6901 acgatccgat aaatgctaac tctaaagttg cgcccataat gatcccagtt tttacatcac
     6961 ctaacacgat tcctgtaaac agccccgtca caatcggacg agaaagcaac gatgtcccta
     7021 gtgcatattc tgattgtgcg acaaatgcaa caattcctaa taagactgct tgtacaatca
     7081 ttgaggttct tcctttctta caaaacttct cttacatcca cttttttatc tgctggtact
     7141 tgacggattt ctacttctat ttgtttgtca attaatcgtt tcaagcgttc ctcctcttct
     7201 ggtaaaatat ttaccgcttt tccgatgctg cgagtaccag gttttgcctt gatccctcct
     7261 aagttgatgg ttttgatttc tggatacgct tctgccagct tatatgcatc ctcgatcgat
     7321 tccactacga tgaataactt atatttatct gtcaccccgc ttttcaacgc ggcaatcgag
     7381 tcatcaattg atttgatcac tagtttgact ccttgtggct tggctaattt gatcgttgtc
     7441 tttcgtatct cattcgtagg tacatcatca ttagcaatca aaatacagtc tgcgcctatt
     7501 gtttgtgtcc atgaaaaagc tacttgtccg tgtaataacc gatggtcgac tcttgtcaat
     7561 acaatcattt ttcttcctcc taaaattcgt cttcttctat tttctgattg agccgctcgt
     7621 tacaaaactg gatcattttt tttgaatcag ttagcacctg ctggatcaaa acagttgtct
     7681 ctacttcagc gtccaaccga gatactagct caatcaatag tggcaaattc aatccggcaa
     7741 cgagatagac atgatcccga ttgatatatt gtaagaattc gttgttgaca cttccgccaa
     7801 aaagatccgt cagaacaatg atttctttat ctttgtacct cataaaaagc tgccttactg
     7861 tttccgctaa atcaaaattc tcatcagtat agcaatcgaa tacagtaata gaatgatttt
     7921 tcccatagat cagctctaat gaatctaata tccctgaagc aaattttcca tgggaagcta
     7981 agataatttc tctatccact ttttcatctc ctcttttcac ttacacatac taaaaaagca
     8041 agttgcatgc caaaattagt ggcaacaact tgcttatgtt caaaattctt cttctatcga
     8101 gagaaactct gtatcttcac tcaaaatatc atagatatag gcaatctcag aattggggat
     8161 catgacacta taatgctctt caatgacact aaacgcagat ttaatagcag ataacccttc
     8221 tttttgacac tgcttccatt tctctaatcc ttgataggtt tcgattggca tttgtcggat
     8281 caatcgttca atgagacagc tcacatggac atacaacgct aattttttgg gattgctgat
     8341 gcttcgattc aactgcacct ctaattctct cataaaatga tcgatttctc ccatcacctt
     8401 ctctgtatct agaatagtca cggaatccag tactttttcc aatgaaaaat tacgaatgat
     8461 gcgctgattg aatatttcct tctctgaagc attcatagta gtgtccaacc attcagctag
     8521 tacagacatc ttttcccccg agatgaggtc ttctaatgaa agatagtcga tattggtgat
     8581 atcaggatct gtggtgccaa tcattcctac gacttcgtat atggaaaata atgggtcact
     8641 gtttttctta tcttttaatt gctgatattc atatgggatg attttcaact gacaagccgt
     8701 aggtaagctt ttttctagca gttcacttaa gtggaccgcc gtaccgattc cagtgaagca
     8761 agtagtaatg agtgctttgg gtttgttttc ctctggatag agcagtcgcc attctggctg
     8821 gatctgtcga acagtctctt ctgctatctc ttctaatgat acattttttt gcaggcattc
     8881 ccctactgtg attgctaaag gtgtcgtcac attattcaaa atcaaaagcg gtacggataa
     8941 ctgtttttta aaaaactgat ggatttcttt cagagatccc atgtctacta atatgatcaa
     9001 tccatttgaa acatcgctgt tttcgcaata atcaaggatt tctgaagcaa tctgctgtgg
     9061 tgtgacatct agtggcatat caaatgattc aaatagatcc tcttgtaata aacgatttac
     9121 tacatttgcc atactgctag cagttgcata accgtgagca gcaatcatcg cttttggcag
     9181 tcgtttttct tttgaccatt tcgctttttt tagatagatc gtcagtaaaa tccgatccat
     9241 acttgtgtct tcaatatcta atttgatctt acttaattcc agaattcgtt ggacatagtg
     9301 ataactagat ggataagact gagaaacttg ttgttctaat tctttgatta gttggataat
     9361 atcatgttct tccggtaacc atttgatagt gcttcgctga tagagatagt aactcaaggc
     9421 atagacactg tttccatcga atttgatttg ataggcagtt tccatttgtc gaaaggtctc
     9481 tcggatagat tgtgtccaat aaaccagcag ctcgtgtttt tcttgtctac tggtctcaaa
     9541 cagcaaaaaa tcaaataatt gaatcacttc ttgctttaat tttccttcgc aagaatatag
     9601 cagacagtta tttttcttga aatcaattag gatttgctca aaacttttaa cgattcgctg
     9661 ctgttcgggc tgttgcttct ccattaattg ctctaaggtc tggtcttgcg taatatagat
     9721 tccttgctgt gctgtatttt tagtaatagg ctgcgcaagt agttcttttt gcaaatgatg
     9781 gatcgtgaca tggatttcag gctgttcacg ctgttcagca tatgcttttg ctgctgaaac
     9841 tttgacgata tttttcagct ctcctatgtt tccggataat ttctgacttg ttaaaagtga
     9901 caaagctggt ccactgactg acaaagggct ccccattttt ctttgttctt cgagaaaaaa
     9961 agagtaaatc agttctagcc gttcattacg actacgctga ttaagtgcag gtagttcgat
    10021 tcggataggt attcttcgaa tgaaagttgt taagaaatga ctagtaatct cttcagtcgt
    10081 agcaaaaaag agacgtgttt tgatccggcg tgacaaatta ggttctccca tacgataaat
    10141 cacaccttga tcaagatagg tgaacagttt ttcctgacct tctggtgtta agcgatgtac
    10201 ctcgtctaaa aacaagatcc caccatctgc tgcttcaaaa gccccttttt tattttcttc
    10261 tgcaccagtg tacgctccct taacatgtcc aaacaaattg ctagtcaaaa gttcaggatt
    10321 atctgcatat tgtgcacaat taaaagtgat gaatggtgca gaatcttcta gcagatcatg
    10381 aagcagacaa tactgataga ccaaattaac taaataactt ttacctgtgc cactttcgcc
    10441 tgtaatcaaa agtggtaatc cgccatctgg atagtaaaga gctgttttga tttgttcgat
    10501 actttctttc aaactggcgt cgtatccgat cataaaagag aataagtctt ttggttgcgc
    10561 gaaaaaaggc tgttcctctt tgagatcatc gatagaaaaa taaaaattcg tgcgtaactt
    10621 aaaaaattgt ttttcaaaag cttctttgtg gaaataaatc actggtcgag aattgatttt
    10681 gactagtagt tgctcatcat ttaactggtt caaataatga ctgactgtgt ttctttttac
    10741 cccaaaatga tcagctaacc tttgtgctgt aaagacctcg ttcatatgtt ctaaatcgaa
    10801 aaaggctgtc tggttctcta agtaacataa aatttccttc ttcaacaact tattcacccc
    10861 tcctccatca tagaaaacgg attcattttg tcaataaaat ctttaataaa gaattgaaaa
    10921 atatatctta ttatagacag cagcctactt atcccttata atttttattc taaaaaacgt
    10981 acagttcctt ccagttttta tactggaatt tcaacatatg aaagaataag gaggctatga
    11041 ctattcaatg aaatcggata aatataaaaa caggaaacat acttcctgga aaacttttcg
    11101 agaaatcatc ggatcttatc tcgctgtagt tgtatttttg cctttgcttt atatggctat
    11161 gacagaacaa actcgtttta ttggcgtaga tacgcaatat atagccgaaa caggattttt
    11221 attcatcttt atcttgattt ttgcgttcta taaatccttc catacaaaat aagattcaca
    11281 gatttcaact ttcctgtctg tttttagcta ttacaaatcc ttatatgtgg tataacaaca
    11341 atttattggc ataatgagga aaaacgagtt atgatgcttt cagagagtaa agaggtactg
    11401 ttgtcaccat cctttttatt gattacgtac accactctta gttttttccc attgattacg
    11461 aaaacaaggt agaaacattg atgttcctac cttgttttct ttttttattt tcagatcaaa
    11521 atctcaagtg gcttttgact tgatgtcggg aggttgtctt cgttccatca aacctgatag
    11581 aatctgcgcc aaaccatagt tttctccact attatttatt cctctaagat agtacccaca
    11641 ataaacgaat aaatattttt ctcattttca tactccttct ttgaaaatca attagatttc
    11701 tctcaaccca cttttttaca aaaattacgt agcaaaaaag attgcaagaa caaattaaaa
    11761 aagaagt
//
LOCUS       DACCYH010000093        11733 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.097, whole genome shotgun sequence.
ACCESSION   DACCYH010000093 DACCYH010000000
VERSION     DACCYH010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.097"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            506..1069
                     /locus_tag="IVY85_002369"
     CDS             506..1069
                     /locus_tag="IVY85_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045369.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1130..1495)
                     /locus_tag="IVY85_002370"
     CDS             complement(1130..1495)
                     /locus_tag="IVY85_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045370.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1571..1816)
                     /locus_tag="IVY85_002371"
     CDS             complement(1571..1816)
                     /locus_tag="IVY85_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8045371.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1886..2140)
                     /locus_tag="IVY85_002372"
     CDS             complement(1886..2140)
                     /locus_tag="IVY85_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045372.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(2304..2627)
                     /locus_tag="IVY85_002373"
     CDS             complement(2304..2627)
                     /locus_tag="IVY85_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045373.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2814..2887
                     /locus_tag="IVY85_002374"
     tRNA            2814..2887
                     /locus_tag="IVY85_002374"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2848..2850,aa:Ile,seq:cat)
     gene            complement(3272..3769)
                     /locus_tag="IVY85_002375"
     CDS             complement(3272..3769)
                     /locus_tag="IVY85_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8045374.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3809..4618)
                     /locus_tag="IVY85_002376"
     CDS             complement(3809..4618)
                     /locus_tag="IVY85_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8045375.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4952..5809
                     /locus_tag="IVY85_002377"
     CDS             4952..5809
                     /locus_tag="IVY85_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8045376.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5802..6704
                     /locus_tag="IVY85_002378"
     CDS             5802..6704
                     /locus_tag="IVY85_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8045377.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6737..7087
                     /locus_tag="IVY85_002379"
     CDS             6737..7087
                     /locus_tag="IVY85_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8045378.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            7121..8059
                     /locus_tag="IVY85_002380"
     CDS             7121..8059
                     /locus_tag="IVY85_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8045379.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            8137..9030
                     /locus_tag="IVY85_002381"
     CDS             8137..9030
                     /locus_tag="IVY85_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8045380.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            9068..9631
                     /gene="amaP"
                     /locus_tag="IVY85_002382"
     CDS             9068..9631
                     /gene="amaP"
                     /locus_tag="IVY85_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8045381.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9645..9833
                     /locus_tag="IVY85_002383"
     CDS             9645..9833
                     /locus_tag="IVY85_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8045382.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9846..10748
                     /locus_tag="IVY85_002384"
     CDS             9846..10748
                     /locus_tag="IVY85_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8045383.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10780..11022
                     /locus_tag="IVY85_002385"
     CDS             10780..11022
                     /locus_tag="IVY85_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8045384.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(11278..11649)
                     /locus_tag="IVY85_002386"
     CDS             complement(11278..11649)
                     /locus_tag="IVY85_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045385.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         4154 a         1982 c         2231 g         3366 t
ORIGIN      
        1 attggtcgtt gaatgggctg aatagtagtg tataaaacaa ttttgtggag cgtgaagctt
       61 ttatggtaga ggaaaatacg aataaagtga tgttgataaa aagattccta tcttgaatat
      121 gaatcgagat gtcttgaaaa tgaatgagtt tgaccaatca ttatcctatc gaaatttaca
      181 gtttcgttcg attggtctgt caattaatga aaaagtaaaa aagaatatgt tctaggaaga
      241 gggcggcaca tctaaatttt taagtaacgg acagttcaaa tatcaagcat tgaaagaggc
      301 tatcctactg tttgaaggaa tacgaatata taaaaacgac aaagaggaaa aagtagttca
      361 ttaaatccct tcaagtgatg aaagaatgag atctaataaa acctgatctt tcatctgttt
      421 ttttaataac gtaataattt ttttattttt tgtgctttcg ccgttattgt agagtgattt
      481 agcattctta tttttaaaaa taactgtgtt atatattaaa aaaggcataa tagttataag
      541 cttttttaaa gaatatttag aaattattaa ttttcgtctg aaaacaaaaa aagaaagcgt
      601 tattatttta gtctattttc agggggggaa ccttatgata aaaaaactat ttttactatc
      661 aatattaacg ctagcaatgg caggttgttc tgactctggc ttagcaactg aactgatcac
      721 aaaatcaact gaagccacga cagaggagtt aaaatgtgga aacgatcaca gtgactacag
      781 aggcttaaat cgacgatgca tgggaatatt aggcagaagc aattgtaagc atcgatgtag
      841 taatcgaaga aggtgtagaa gaagcaacag aaaaatcaaa agaaagatca aaagaagcaa
      901 tctgtgctgt gcatcgtata cagacgtcga agatgcggta cttttaaacc acgtcaggag
      961 atgttcattt atgtctaggg aagttggcga tgaaccaaaa ctagagtacg cttttagttt
     1021 tgcacgtgag gatgaagata accctaaaac ttacgacatt ttagcttgat ctaagatggt
     1081 taaagctttt taatgaagta agaggagata gaaggctttt ctagttatat taggtaagat
     1141 aaattccttt ttgctccaaa tttgctaaac attctgctag ataaacttca tcgtgttccg
     1201 gataaatcgg cacgttgcgc tctacagtag gtaaatcaaa aaatgcttcg catgatttgc
     1261 cacgatacat aggatttttc cactctggtg ctgggctgta accacgtttt tccatttcat
     1321 ccatgacaag ctgatgatat tggaataatc tataaggaga atagtgaaaa acataatcca
     1381 cagtagcatg tttctttccc cagccatttc ctcttaatgc acagcattcc cgatgttgtc
     1441 ctaaaagctg ttgacgagga agctttgaaa tcaatttttc atgccataat cgcatagaaa
     1501 tgtctccttt atatatagat tgttaacctt agacgttaaa tgaaaaaacc agcaccaagg
     1561 ctggaaaaaa tcacttcagt ttgcttgctg tttctgagcg accaattagg taatcaacgg
     1621 aaatatcgta aaagtcagcg atatagatta attcaatgat gcttggaatt tcttctcctt
     1681 cttcgtactt acgatatttg aataaaggaa tacttaacgc gctagaaaca gctacttgag
     1741 ataaagatcg gccagttctt aagaaattca gacgacaacg taaagtatcc agtaaaatag
     1801 cttcttcgat actcatttaa tcacctttca ttatagtaga atagttataa tgactataga
     1861 aagctacaca tgcaatcaat agtactcaat gttttttatt taaacgtatc aaataaagaa
     1921 tgacgatcaa taaagacaaa accagtcctg ataattcttc tataaaaaca ctatcgggtt
     1981 caaagtagtt gtaattaaaa actttccaca taaagtagat agccggaaga gtcaaaatag
     2041 ctaaataaaa ccgttctttg ttccaattca taagataaac aatacttgcg accagtgcga
     2101 ccgcaggcac ccaaaacaaa actctcacca acgtaatcat gtaacggact cctcaacgat
     2161 tcatataata aaagataaca tgttattgca tacatcacaa atgaaatgct aggaaaataa
     2221 aagaagaata gatcaatgaa aaatgaaaaa gcagaacagt tttctgctga taagcaggaa
     2281 ctgttccgct gaatgtgtct tatctatcga gaaaaaatca atggattgat atctaagtaa
     2341 ttcaatacat ataaaaagag gacgatcgca ttcactaaaa aggaaagaat gatgatccca
     2401 tagcgaatgg aaacatattt aacaaatact tttgtttgaa tacgggcata tctttttcct
     2461 ttatagctga gcatcatgac aagaagcaaa agggtaatca caccttgaaa aatcattcca
     2521 ataaacagaa ttcctgaggt ttgataattt aattggagaa aataagctgc tgccgcaact
     2581 aaatcgaata aaatcgcaat aaatggcaaa ataatcactc ctaacattga gaataaataa
     2641 aagaataaca ttttttctaa aataagcaaa taaaaattaa agtaacaaaa tagttatgac
     2701 acaagtaaaa gaaataagaa taacaatatt attttactta tattttgaaa cttgtgagag
     2761 tttgtcattc gtgctatagt tgaaaagtaa caaaaataaa ttttatttat gatgtacctt
     2821 tagctcagtt ggttagagca gacggctcat aaccgtccgg tcgtaggttc gagtcctaca
     2881 aggtacataa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaagtaa
     2941 aaacagcttc ttatgttttt acttggctgt tcttattact gcagatgcaa gcttttgaat
     3001 accgtttaag gaagaggttg tgaaatcagc gttttgacct gagtattagc ttaacaaacg
     3061 gaaaaatagt tcctcatatt ttgccgtttg ttaagctaat accgagggtc agctgattga
     3121 acaccattta agaaagaggt cgtgagcctg ccgtttcact ctgtgtcata aaaaagccaa
     3181 tctactttga tagaaaaagc acgtaggact aaaacagttc tgcgtgcttt ttttattgtg
     3241 tgttaaatat cgtaaatgaa gatagatatt tttatgattc gacgatagag acgttgaaat
     3301 ctttcaacag ctctaaagcg gattctccgt gttttctact ggagactaac attggatcaa
     3361 caaaaaagtc tgcttcagga aaagcactct gcaggcctat cagattagag agtacacaca
     3421 agtttgtttc aatcccgact acttcgatct ctttttgttc ttctttgctt tcaaataaag
     3481 tttcctggat ttcagctaaa gtttcaggtg gaatcgtaaa gtaatacttt ttaatcactc
     3541 gttcattttc ttgtggagaa agaagaggga tcaattgcaa atcttctcgt tcttcttctt
     3601 tgtccttcag ttcaactggt atatcccgtg tgaagagaat atattcatta ttttggcgtg
     3661 ctttagctaa tctttgagca atacgttctg ccaattgatc tgactctgga atataaaatt
     3721 cgctgtttgg atcaagaata tgattttgca tatcaataat aactaacatt ttctttcctc
     3781 ctacattcat atcgtcattt tattcttttt aatagaagtt ttttaaaata taagcaacac
     3841 cagcttcttc attcgtcagt gttacttggt ttgcaatttc ttttacttgt cgctgtgcat
     3901 tccccatcgc aatactcaac ccgctgtaat tcaacatagc aatgtcgttt tcttgatcgc
     3961 caaaagccat gatgtcactc gattcgattc caaggtaatc cgccagaaaa acaagtgaat
     4021 gagctttagt gatacctgta ggcgtgattt ctaagaaatg cggatcagag aatactgatt
     4081 ggataccatc tgcttgcaga tctctttgta gcttctgtag tttttcttca ctcttatctg
     4141 ttaatgctaa tttaagatag ctaagagaag gatcttccaa catatttaaa atttgatcaa
     4201 aagaatggtt ctgtaaattg aaatgttcat aataagggtc atctttgact agatttgatt
     4261 catcgaatgt cagctgatca tcttgggttt ctcggctagc aatgatggat attttcgctt
     4321 cttgagcata gaagaaggct tttctcagtg tatcgctatc tatttttgaa cgtctgagaa
     4381 ctttcgccat ttttccatcc ttcatttcag caatcagtga accattggaa gaaatcaaat
     4441 agccaccgat tcctaattcg gaaaaaatat ctttagcaga aatgctgttc cttccggtag
     4501 cgacggttac gattccgcca ttttcatcaa atttccgaag tgcttggata ttttcttctg
     4561 gtatctttcc atttcgatca agcaaggtac cgtctaaatc aatcgctgct agtttcattt
     4621 tatcttccta ctttcttttt acatatctat tttcctgtta ccttaagatt aaatgagaat
     4681 ttaaaaaaat gaaactaact tgctttgtca ttatgaattt tttcaaaaaa cattcttaag
     4741 aaaaaggtgg ctatatatag tagtttttgg tcaatacctt tgaataaaaa gccatgaaaa
     4801 acgaatagtt agcgcttacc gatgcgtttt gtgataaaaa aattttaaaa tattcaatga
     4861 aaacaaagca tatattttgc tttaaaacca aatgaggaat attctagatg tagaacaggt
     4921 tagaaaaaga ttagaaaaag gagtgatttt gatggagaag agatacggcg tagttcctgt
     4981 ggtaactgct tcagacgaaa attatgctcc ttacttgagc gtgatgatcg caactgcatt
     5041 agaaaattgc aataaaacaa gacgcataaa attttatgtg attgacgatg gtctatcaga
     5101 atatagtaaa gaaggattgg aagaaacggt aaataaatat tcaagtaatg caagtatcca
     5161 attcctgaca gtggaaaaag atatctacga agacttccta gtcagtgacc atatcacaac
     5221 taccgcttac ttaagaattt ctttaccaaa cttattagct aaagaggatt ataagaaagt
     5281 cctttactta gattcagatg tgttagtact agacgatatc gtaaaactat atgatgagcc
     5341 gttaaatgga aaaacaatcg gcgctattat cgatccaggc caagtgaaag ctttggaaag
     5401 attaggaatc gattccgatg acttgtactt caattcagga gtgatggtga ttgacattga
     5461 tcaatggaat aaaaaagaaa ttacagaaaa aactatccat tacctcagtg aaaatggcga
     5521 tcgaatcatc tatcacgacc aagatgcttt gaatgctgta ctttatgaag attgggaaca
     5581 acttcatcca aaatggaaca tgcagacttc gctgattttt gaacgtcatc cagcaccaaa
     5641 tgaaaaatat gaacgtcagt ataaagaagg aaatgaaaaa ccttcgatcg ttcactttac
     5701 aggacatgat aaaccttgga atacgttgaa agatcatccg tataccaatt tatacttaaa
     5761 aaaattagca cacagtactc taacgaaagt aggcgagatc aatgaataaa aaagaaatag
     5821 cagtagtcgc aagttgtaac acaaaattcg taccacattt agctgcatta tttgtctcag
     5881 tattagataa ttgcaacccg tctaaatttg ttcggttcta tgtgattgac gatgatatcg
     5941 attttgaaag taaacagctt ttacgcttct cagtgaaaaa cgcacgaatg aactcagatg
     6001 tggaattctt gaaaatcaat aaagaattct ttacaaatgt ggtaatcagc gatcgtattc
     6061 cagaaacagc ttattatcgt attgcgattc cggaattgtt ccgcggcaca gaagttgaac
     6121 gtatcttata tatggattgc gacatgatcg ctttacaaga tatctctaaa ttatggcgtc
     6181 ttgacttcgg tgattcaatc gtagcagctg ttgaagacgc tgggttccat caacgactgg
     6241 aaaaaatgaa aatttcagct aaatcaatgc gttatttcaa ctcaggtttg atgctgatca
     6301 atgtgaaaaa atggttagat gaaaatatta cgcaaaaagt attagatttc atcgaacata
     6361 atccagaaaa attacgattc catgaccaag atgcattaaa tgctatctta catgaccgtt
     6421 ggttaccact tcatccgcgt tggaatgcac aaggctatat catggcaaaa gcaaaaaaac
     6481 atccaacagc agcaggtgaa cgtgaatacg aagaaactcg caacaatcct tacattatcc
     6541 atttttcagg acatgtgaaa ccgtggagca aggattttga agggccaacg aagaaatatt
     6601 acgaaaaata tgcagggatg acggctttcc gttgtgtagc taaattccct aaatatccaa
     6661 aatatgcaaa agttcaacca aaaacagatg tagttgttaa ataatagaag accacaactg
     6721 aagaggggga gtaggaatgg aaattacaat gtctaaaaat gcagtagaga cgttaattga
     6781 aaaggttggg gaaaatacaa aaatcgcact cgcgcttatc aatgattccg atccatttct
     6841 aagggacaaa ggtgcttttg caaaaggcag cttttttcaa atcattcctt ttgtttcgga
     6901 atttggagaa tatgcgacta aaatcgagca tccattattg gacatctata catctaaatt
     6961 agagcaaaat tatttcggta aacgattgaa catggacttc aacaaacagc ttgacagctt
     7021 ttcattagaa aatgaaatcg cagtactcga ttataatatc aaattaaaga actgcttctt
     7081 cagctaatag tttcgagaaa gtccaaaaag gaggtataca ttgagatact actttgtgga
     7141 tgaagaaaag aatgtaatag aaacagatga aaaaaaatat aattggctcg tacttgatag
     7201 tgcaaagccc gaagagattg agcaagcaat cgaagaattc gagcttcctg atgatatttt
     7261 cgtaggaacc gaatatccgg aagaagtttc tcggatggag cgcctttatg agacgaaact
     7321 agagcatcca ttttctttgg tagtgattaa tctagatcag tcagataatc gtatcgaaaa
     7381 aaaattaacg ccaatatctt ttgtactttc cgataatctc ttgattactt gtatcgatag
     7441 aaaaaccgaa tttgtggatc ggctgctgac aaagcatgga aatcatttaa actcgtttga
     7501 aaagatcatc acgtatacat tattagatat ttacacccat tacgtgaaag aactaagaga
     7561 gatgaaaaag cgaattgacg ctttggataa agcagctcgt aaaacaacgg aaaatgaaga
     7621 actgtataaa caggcagatc ttgaaagaga catcgtatat atcgatcata ccttaagaga
     7681 tcaacgggaa acaatggatc aattatggaa ttctgaaact ttcatcaaac gtttagacga
     7741 tgaacagcta ctctatgatg tccatttgag acagcgacaa acagaaaaaa tgatcgagat
     7801 ctatcgagat ttattagaga gtattggtga tctattcaac gggatgatgg acaataatct
     7861 aaatcatatc atgaaatatc tagattcagc tgcattaatc atttccgttc cggcgttgtt
     7921 cgcagggatt tggggaatga atacaggagg attaccgggt aaaagttctg ctctaggctt
     7981 tatactcgta gtctttggct ctttgatagc agcagtagtt ttaggttacc atttataccg
     8041 gaaagattat acaaattaaa gaatctattt aaaacaggta tcaaacaaag aaatttgttt
     8101 tggcaataca atttaaaaac taggaggaat acagcaatga cagaaccaaa attaatcgac
     8161 ccgcgaaact tgtaccatac ggagaaattt cctaaacaag atcaggatac accagcttta
     8221 cagcagaata tgattcctgt tcctgattgt ggagaagaat cctatgaagg caataatcaa
     8281 ctagaaaacc gccgtgtact aatcactggt ggagattcag ggatcggtcg tgcagcagcg
     8341 attgcatttg ctagagaagg ggcagatatc gctcttcaat tcttcccagg agaagaaaaa
     8401 gatgcagagg aagtagcaaa atacgtgaaa gatgcaggac gtaagattgt cttactacct
     8461 tttgatcttc gtgacgaaca agcacctaaa gaaatcatag ataaagccgt gagtgaactt
     8521 ggcgggctag atacgatggt actaaacgca ggacagcaaa tttcttgtca gtctattgct
     8581 gagttaccac ttgagcaagt caaagacaca tttatgataa acatcatcgc tatgtttgct
     8641 ttagtgaaag aagctgttcc gcatatccca gcaggaggag caatcgtaac aacaacttct
     8701 gttcaagcat tcaatccaag cgaacattta ttagattacg cagcaacaaa agcttcaatc
     8761 gctaacttta cgatcggttt agcaaaacag cttgcaccta aaggaatccg tgtaaatggt
     8821 gtagcacctg gtccaatctg gacaccatta caactagacg aaggacaacc ttctgattcg
     8881 atccctgaat ttggtcagca aagtttacta gaacgtgcag gtcagccagg agaattagca
     8941 ccagtttatg ttttcttagc atcgaataaa gcaagttatg ttacagccca aatttacgga
     9001 gtaactggtg gagaagcgat caacttataa gaagttgatc gtttcacccc tatgaggagg
     9061 tgcgaaaatg aataaaggtg taaaaacgat cggagtgatc ggaacattac ttttgctatc
     9121 tgtgcttttg tttgcgatac tattaagtgc aacctatcca ttgccttttc agctagagaa
     9181 gtttcgattc ttttccatca ctaattcata cgtgcaacaa tatgtatttt ggcttgccgt
     9241 tgcatttgct attttgacaa ttatcacgtt acttgttttc ctgttttatc caaaaacaat
     9301 cggaaacttt ctactgaaac atggtgatgg ggagttgacg ttggataaaa aagcaattga
     9361 aggaatggtt cgttctcact tgcatgaaga tgaattcatc cattcaccaa aagtaaatgt
     9421 caaagcaacg aaaaacaaga tcgccgtcaa tgtaaaaggg gagttgaaac gaacttcagc
     9481 actcattggt aaaacaggaa cattgatgaa agaaatcgaa aatgaagtac aacacatatt
     9541 gggaacaaaa gagccagtga aagtatcggt gaaatacaca ggctttgaag aaccagctaa
     9601 acgaagcaat gaccgcaaac gagtagaata gggggggacc aaaaatgaaa gaactatttt
     9661 cgacatacaa gctccctatc atctttgggg tacttggatt gatattagcc gttttatgga
     9721 tcaccgtggg ctttttcaag accttattga tcttgatcat ggcagcgatc ggcgtcgcta
     9781 tcggttttta tctgaaacaa acgaaaatct tagatcacta ttttaaatct taggaggaaa
     9841 catccatgaa taacaataac aaaaataata acaataaaat gaacaaccaa aaaccagtgt
     9901 ctgcacatcc agacccaacg acaccaaacc aatcaaatgg tgtgaacgat gaaaagaaca
     9961 aaacagcacc agtatctgca catccagatc cagtagtccc taacgttcaa caaggagtag
    10021 aagagaagaa acatgatgca gaaaagcatg cacctcatca tccaggaact ccagaatcta
    10081 aagttccaga acaacatgat gcaatcgatc cttctaaacc tgtggaagag caaaaacatc
    10141 cttctgacca tgcgaaagca caagaaaaac ttagttctga ccacacaaac ggacaaggaa
    10201 aacttggttc tgatcataca aataaccaag gaaagcatga ttctgaacag gaagattcaa
    10261 tgcgcggtga gctgaattat gaagataaag ttgtgcaaaa aatcatcgga atcgcaattg
    10321 aacaagtaga cggtttatta tctgcaaacg gtggtttctt ctctaatgtg gcaggaaaac
    10381 ttgtgaatac agataatgta acggctggga tcgaaacaga agtcggtaaa aaacaagtag
    10441 cagttgatat ggatgtcatt gtggaatatg gcaaagatat cgaaaaaatc tttgaagaaa
    10501 tgcaagaagt aatcggcaaa gaagtaaaaa atatgacaca tttagaagtg atcgaagtca
    10561 acgctaacgt agtggacatc aaaacaaaag aagaatttga aaaagatcaa gaaacggtgc
    10621 aagacaaagt aactgacgca gcgaaaaaaa ccggtgaatt tgcttcgaat caaacagata
    10681 aagtaaaatc agcagctgga agcggcgcac aaaaagtaaa agaaaataca gaaccacggg
    10741 ttcagtaata aggaagtaaa acattaggag gaacgaaaca tgggatttat ttggtcactt
    10801 atcgtcggag gaattatcgg agcaattgca ggagcaatca cgaataaagg ttcttcgatg
    10861 gggattattg caaatgtcgt agcaggttta gttggatcag cacttggtca agcattatta
    10921 ggttcttggg gaccaagtat tgcaggaatg gcaatcgtgc catcattgat tggtgccatc
    10981 attttagtac taatcgtatc attctttgct cgaaaactgt aaaaagactt ttaaaaagag
    11041 gtcgtgaaaa agctgttctt catcaagtaa taagaacagc caaacaaaaa tagcttctca
    11101 tatttttcat ttggttgggc atattatcga agatgcaagc ttttgaacac tgtttatacg
    11161 gaataagagg ctgtgacaag acttttgtca cagcctctta tttatgcggg caaattccca
    11221 attagatttt taaaagtttt tccttaaatg tgtctttatt gatacagaga agcttcttta
    11281 ataaaacaat gcctccctaa attttcttga gataggaaag gtgaacatac aaaataatac
    11341 gaccactttg aaaacccaga tgatccacaa atggatcgta tgatactgat gaagtaaaaa
    11401 gcaatggatc agaaactcaa agagattgaa aaagacaagg aagaaataat atctcttttt
    11461 tactcttgtc tgttttagcc aataatagct cgtaaagtaa tctagacaaa caaatagaag
    11521 taaacaaagt ttcgtaagag cagcaatcgt atcatgttgc tctgctagac gattgacgag
    11581 caggaaaaat gttggtaatg gaatatagat cacgagtaat gaaagaaaaa agtaaacaat
    11641 aaatggcata ctattcctcc taattgtgtg agttcgccct cattttaaaa taacgatatt
    11701 tgtttgtgaa gctttttcgt gtcgtaacaa atg
//
LOCUS       DACCYH010000094        11633 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.098, whole genome shotgun sequence.
ACCESSION   DACCYH010000094 DACCYH010000000
VERSION     DACCYH010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.098"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            24..803
                     /locus_tag="IVY85_002387"
     CDS             24..803
                     /locus_tag="IVY85_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045386.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
     gene            849..1793
                     /locus_tag="IVY85_002388"
     CDS             849..1793
                     /locus_tag="IVY85_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8045387.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1809..2588
                     /locus_tag="IVY85_002389"
     CDS             1809..2588
                     /locus_tag="IVY85_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP8045388.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            2600..3298
                     /locus_tag="IVY85_002390"
     CDS             2600..3298
                     /locus_tag="IVY85_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP8045389.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3326..4090
                     /locus_tag="IVY85_002391"
     CDS             3326..4090
                     /locus_tag="IVY85_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP8045390.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMTASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPDYEEVKKKKFGLF"
     gene            4104..5921
                     /locus_tag="IVY85_002392"
     CDS             4104..5921
                     /locus_tag="IVY85_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8045391.1"
                     /translation="MFILTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVSIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFSSLSASAASSILILLFINRRYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNR"
     gene            5975..6898
                     /locus_tag="IVY85_002393"
     CDS             5975..6898
                     /locus_tag="IVY85_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HAP8045392.1"
                     /translation="MNAFKTALNNNEMEGKIIGADCSELAPALYMCDEKIVLPKVSES
                     NYIEELIKVIKFFEIKLIVPTIDTELSTLSRNKKYIENETDSVVLVSSEKVIDICENK
                     ILTSAFFEQNGFKTPEILNSSEITKFPVFIKPLNGSSSVNTFIVNNEKELLFFSEYID
                     NPLIQEYILGEEYTIDVCLNFDSTPVSIVPRKRIATRGGEILKGQIIKDRELIETTKK
                     MLEKLNAIGHITIQCIKNEKGIFYLEINARYGGGAPISIKSGANSPEYIIKMIDSKKN
                     LRYRENYLDKMIALRYDQAIYLDEKGNVYND"
     gene            6870..7586
                     /locus_tag="IVY85_002394"
     CDS             6870..7586
                     /locus_tag="IVY85_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP8045393.1"
                     /translation="MKKEMYIMIKAVIFDLDDTLVSEYDYVKSGYKCVARRLAVQKIF
                     PATFDEIYRVLLDLFAEDSKNVFNRLYDYFEIPYSKKEIKELVNVYREHKPDINFLEN
                     ADTLLTELRSRGYKLGIITDGYAITQRNKLDALKVDYLVDYICITDELGRELWKPHPI
                     SYLLLLEKFNLKAQECIYIGDNELKDFITAKSIGMQTIKIERVNGIYRESSLKFKRFY
                     QADKKIRSLWEIKNFLGVIS"
     gene            7583..8701
                     /locus_tag="IVY85_002395"
     CDS             7583..8701
                     /locus_tag="IVY85_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP8045394.1"
                     /translation="MKSKRILLASPHMSEEGYEKEYVKEAFDTNWVAPLGANVNGFEK
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITIKGINANHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGD
                     DVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            8694..9311
                     /locus_tag="IVY85_002396"
     CDS             8694..9311
                     /locus_tag="IVY85_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8045395.1"
                     /translation="MRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLGS
                     PIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKMLRATSLDELPE
                     LWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKFD
                     LDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNIPEKFKGS"
     gene            9438..10607
                     /locus_tag="IVY85_002397"
     CDS             9438..10607
                     /locus_tag="IVY85_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8045396.1"
                     /translation="MCRVLNILFQYEKIIKKYKEKRVMAKYYNKKILIMANNDDGLYR
                     FRGMLIKNLVDQGNEVIVSLPKGKNIPKLIELGCQIIETPIDRRGINPKTDFKLFFNY
                     CNILKKIKPDMVIAYTIKPNIYGGLACRLRKVSYALNITGLGTAFQKKGVLKSLIVFL
                     YRLAAKKSKVIFFENFENMKTFQTLQIAKQSKYKLLNGAGVDVEHFSYLEYPKDEQVH
                     FLFIGRIMKEKGVDELFEAMKNLYSEEKNCVLDVLGYFEEDYGIKIEKYEKEGWLKYH
                     GYQSDIRPFVERAHCFVLPSWHEGMANTNLESAASGRPIITSDIPGCKEAVIESKSGY
                     LCERKNSCSLYNSMNKFLKLSYEQRINMGKMGRNYMIKKFNKKKVVDETIKSLFS"
     gene            10614..11009
                     /locus_tag="IVY85_002398"
     CDS             10614..11009
                     /locus_tag="IVY85_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8045397.1"
                     /translation="MFCKVCDRKEGFCGFRIDIKELAKVSDIFLFTTLQEGMPRSMME
                     AMASGLPCIASKIRGNVDLLVEGKGGYLVPAKDAELVAECLRELINNKSLRKYMGNEN
                     LSRIKEFEVSIVKKRIKEIYEEVIGESVK"
     gene            11006..>11633
                     /locus_tag="IVY85_002399"
     CDS             11006..>11633
                     /locus_tag="IVY85_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8045398.1"
                     /translation="MKNPMFSIIVPIYCVEKYLKDCLDSIRNQTFKNFEAILVDDGSP
                     DECPEICDYYSQIDGRLKVIHKKNGGIIDARKTGVTSAMGEYIVFLDGDDWLANTYLE
                     KYAEVITKYNPDIICSGYFASRSNKIRKRELSCSFGYYDKDKIINLIYPKLIEDEHGE
                     YFRPQLWAKAFRTTLYKEQQQVSGQVSVGEDHACTKPCIYHANSIYMME"
BASE COUNT         4273 a         1765 c         2328 g         3267 t
ORIGIN      
        1 catcaatact taggagtgaa tctatggaaa agccattcgt caagtcattg actgctgtgg
       61 cggtaggtgc aggtgctatt gcgatctgtt tgtttggtta ccatatcaat aatcagcggc
      121 aacatcagca acggatcaat tatgcagaat ctgccatcac caatcaaaaa gacaccttga
      181 cttctttatc aaaagaagta gacaagctgt atagtacaaa agaaaaaaat tttctaaacc
      241 ctgaaataac agaagaaaca atcacaagtt tgtcaaataa gctatcctct gtcaaacttt
      301 cagcagatga ttacgacatc aaagaatcag aattgcctaa agaagctgtt gcaatccaag
      361 atgagaagaa agcaatcgtg acacagttag atagtgcaga atcaaaactg aagatccaga
      421 cagctgtcaa cgatttattt actaaaaatg tatcaaactg gcagcaagct gtagacgatg
      481 tcgtcatcaa agaaaaggtg acaagtgcag atgtcgctcg tgtacgtgaa aatatgagct
      541 tttttaaaga tagtccttgg aaaacggttg ttatgcaata tctaggattc gctgatactc
      601 agatcgcgca agtcacgcaa ctagatcagt tattcgatac gatgttgaag gatggtcaag
      661 tgactgccac cgctacttac gatcaatatt tgacggcgct cagtcaaata gagcagatcc
      721 ggaatgaaaa aataagcgca gcatacgcaa caaaagcaca aactgttgct cagcaaatgg
      781 gctatagcaa tataagttat tgacagttaa aacgttgatt caagcaaggt tagcaaacgg
      841 aggaaactat ggcaagacga agaagaaaat caagagggaa aaaaatattt ctgattattt
      901 tagcagttct tatcgtatta gtattagcaa ttgtaggagt aggagcaaaa ttatatctgg
      961 atctctcaca ttctattgag aaaacttacg aatcagtgga acgaacccaa ggagaacgaa
     1021 cgaaaccagt tgatctggac aaacaagaat ctttctctgt tctattgatg ggcattgata
     1081 ctggagattt aggtcgggtc gatcaaggaa gatcagatac gatgatggtt gccacagtca
     1141 gtcctgaaga caaacagaca acgatcgtca gcattcctcg agatacgtat gtcgacatcg
     1201 taggtcgtga cacaaaagac aagatcaacc acgcttacgc attcggcgga gctgccatgg
     1261 cgatggatac cctacaaaaa tatgtagaca tcccaatcga tcactatgtc tctatcaata
     1321 tgaaagggct aaaggaactc gttgatgcag ttggtggtat cgaagtcaat aatgacctca
     1381 ctttctcaca agatggctat gactttccaa tcggtaaaat tacattgaac ggggatcagg
     1441 cattatctta ttctcgtatg cgttatgaag atccaaatgg tgactatggt agacaagagc
     1501 gtcaaagaaa gatcgtagaa ggtgtagcaa aaaaagtatt gagtttaaac ggtgtcagca
     1561 actatcaatc cgttttgaat gctctagagt cgaatatgaa aacagacatg agctttgatg
     1621 aaatgaaaaa aatcgcttta gattatcggg aggcatttgg tacgatcaag caagatcagc
     1681 tgcaaggaga aggcttcatg caagataata tctcctatca aaaagtcagc gatcaagaac
     1741 ttgatcgtgt gaaaaaagaa ttaaaagatc aaatgaatct agagaataaa taggaaagtg
     1801 gagaaaaaat ggaagagaca atcagcttga aagaaatttt tgaaacatta cgcaaacact
     1861 tggtaacaat catcatctca atgtttgctg gcttggccat ctcaggaatc gtaacatttt
     1921 tcatcttgac accaaaatac agttcacaag cacaattgat cgtgacactg ccacaatcag
     1981 acacagcaaa tgtcaatgat gtcaatacga acctgcaaat gatcaatacg tataaagata
     2041 tgatcgtcag tgatttagta ctaaatgaag tcaaagatcg tttagaaaca gaagacaatg
     2101 tgaaaatgac tgctggacaa atcaaagatg cgatctccgt caatcagtca caaaattcac
     2161 aaatgttctc gatccaggca ataagcacga atgcagtgac tgctcaacag atcgctaaca
     2221 caacttcaca agttttccaa gagaacgcaa aagacgtgat gaatgtcgat aaaatctcga
     2281 ttatttcagg tgcagttgcc aatacgacac ctgtttcgcc aaataataaa ttgaatctag
     2341 tgattggctt agtattagga atgatggtag gcgtaggtct ggcattctta ttcgaattac
     2401 ttgatcggac agtgaaagat gaaaaatatg tgacggatgc attaggattc ccaatcttag
     2461 gaacagttcc agaaatgtca gcgaaagaat tgaacgctgg tatccgtaaa aaaggaacag
     2521 ctcctgcacg accaaaagct acaaacgata aaacagcccc atcacgcaga agccgctcaa
     2581 gagtctagga ggaagaaaaa tggcacgaac acagaaacaa aaagcagcac aaactcatgc
     2641 agtcagtttg atcacattaa caaacccatc ttcaccaatc gccgaacaat atcgtacgat
     2701 ccggacgaat atccagtttg cctccgcagc tggtcagcaa atcaaaacga ttgtcgtgac
     2761 ttcatcaggt cctggagaag gaaaatccac cactgcagca aacattgcag tcgtttttgc
     2821 taaatccggt caacgagttc tactagtcga tgcagattta cgtaaacccg tcgtctataa
     2881 gacattccag ctgaacaatg ccaggggatt aagtacagca cttagttcat caggaagtgt
     2941 agcagatgta atccaacgaa cgccagtcga gaacttgtcg attttgccta gtggacctaa
     3001 gccacccaac ccatcagaat tgttaagttc gccaagaatg gatcagatcc tagacgaagc
     3061 aagacagtta tttgacgtgg tgatattcga tatgccacca gtagtagccg taacagatgc
     3121 acagatcatg tcttcaaaaa cggatggcac tttattggtc gtacgtgaaa atacatcacg
     3181 taaagaatca ttgaataaag caaaagaact gctagagatg gtacaagcac gagttttagg
     3241 tgttgtatac aacggagcag aacatagcaa agatgctggt tattactacg gaaattaagg
     3301 aataagaaga gaggagacaa gagaaatgat cgatctacat tgccatatcc taccaggaat
     3361 cgatgacggg gcagaaaact tagaagcaag tattgcaatg gctgaaaaag cgattcagca
     3421 aggcatcacg catattttat gcacaccaca ccacaataat ggaaaataca gcaatgaaaa
     3481 atcacaagtc atttctctag tcgcttcgtt acaagccgaa ttagagaaaa gacagttgcc
     3541 tttgacgtta ttggaaggac aagaagtacg tattaccgga acgctcatcg aagacatcca
     3601 ccgagatgaa attttattta cagatttaga tgatacatat cttctgatcg aatttccgac
     3661 actggaagtg ccgttatatg cagaacggct tttttttgag ttgttagaac taggaaagac
     3721 accagtgatc gtccatccag aacgcaacgc gcattttcgc gaagacccga atcatttgct
     3781 tccgttccta gatatggggt gtttggccca acttactgcg ccaagttatg ttggggtatt
     3841 tggcaaagat attcagaaaa cagctaaaac aatggtcaaa cataatctcg tgcagatgac
     3901 tgcttctgat gcacacggag tctctaagcg caccttttac ttaaaagagt gctatgagca
     3961 gattgccaag gactttggta aggaaaaagt agtacagatg aagcaagttg caaaggatct
     4021 gatcaacggg gatgaaatca gttatccaga ctatgaagag gtaaaaaaga agaagtttgg
     4081 tttattttag ggggaattaa aaagtgttta tattaacaag aagaagaaaa attgccacat
     4141 tggtttttgt ggacagcttg ttattaattg ctgcaaatat tgcagcagct aaattcatga
     4201 agccgtttgt atctatacca atggatctta ttttaatctc tatcggtttg tcgattggtt
     4261 tttatttatt ttatggtagt ttattcaaag ttttcactcg gatcaatcgc tatacaaatt
     4321 taagagagat cattgcaatt tttagttcac tatctgcaag tgctgcctca agtatcctca
     4381 tactgctgtt cattaatcgg cgatacagtt tacggttagt tatctttgct tatctgcttt
     4441 cgctcttatt gatcatcggt agccgattga tctggcgtat ctacatcgaa acaaaaaata
     4501 tgagatacgt atcagcagat tctgctaaaa atacattgat tgttggtgct ggcgaaggcg
     4561 ggcgtatttt atacaacagc tttcttggat ccaaaacggc tcaagatatc cacgttgtcg
     4621 ggtttgtaga tgatgatcca aataaaagaa atacgtatct ttcaggaaaa aaagtattag
     4681 gtgcattaaa agatattccg gaattgattg aaaaatatga cattcaaatg gtaacgatcg
     4741 ctatcccttc cttatcaaga aaaaaattac gacgaatctt cgagttagtc gaatccgctc
     4801 gtgtgaaagt caacacgatg ccttctatag aagaacttgc ttcagggaaa attagcgtat
     4861 caaaattaaa aacaattgac gtagtggatt tactgggaag agatgaggta aagctggata
     4921 ttgaatccat caaagatcag atcacagaca aagtcattct tgtcaccggc gcaggaggat
     4981 cgatcggttc agaaatctgt cggcaaatca ttcaattcaa tcctgcaaag ctattgcttt
     5041 taggacatgg ggagaactcg atctacttga ttgaccgtga gttacgcacg catcatcaaa
     5101 attgtcctac agaaatcgta ccgatcatcg cagatatcca agatcgcgag aaaatcaatg
     5161 agatcatgga acaatatcat ccagatatcg tctaccatgc agctgctcat aaacatgtac
     5221 cactgatgga atataatcca aaagaagcag tcaaaaacaa tattttcggt acaaaaaatg
     5281 tagcagaagc agctaaagca gcaaaagtga aaaacttcgt catggtatcg acagataaag
     5341 ctaacaatcc tccaaatgtc atgggttcaa caaaacgaat cgctgaaatg attgtgactg
     5401 gattgaatga agaaggctgt acgaaattct cagctgtcag atttgggaat gtccttggtt
     5461 ctcgcggtag tgtgattcca gtattcagag aacaaattgc tcaaggtggt ccaatcactg
     5521 taacagactt tcgtatgact cgatacttta tgacgattcc tgaagctagt cgtttagtta
     5581 ttcaatctgg cgcattagca aaaggtggcg aaatttttat tctagatatg agtgaaccag
     5641 tgaagattgt agacctagcg aaaaacatga tccgattgag cgggtatagc gaagacgaaa
     5701 tcgaaatcat tgaaacaggt atccgtccag gggaaaaact atatgaagaa ctattgttgg
     5761 ataaggaaag aaatgatgaa gcagtttatg agaaaatctt tgtaggaaac atcaaaggtt
     5821 actcaataca aaaagtgatg gattttgtca agagcctgcc gcaagacgat gaacaattgg
     5881 ctaaagacat tgtcactttt gcaaatgctt cgaatagata ggagattaaa aattgaatat
     5941 tttaatatta agttgtggac gtcgttgtga actagtgaat gcatttaaaa cagcattaaa
     6001 caataatgaa atggaaggaa aaatcattgg agcagattgc tctgaactag cacccgcact
     6061 atatatgtgt gacgaaaaaa tagtattacc taaagtttca gaaagtaatt atattgagga
     6121 attaataaaa gtaataaagt tttttgaaat taaattaatt gttcctacta ttgatactga
     6181 attatcaaca ttatcaagaa ataaaaaata tattgaaaat gaaacagact cagttgtatt
     6241 agtttcttca gaaaaagtca tcgacatttg tgagaacaaa attttaactt ctgctttttt
     6301 tgaacaaaat ggttttaaaa ctcctgaaat tttaaactct tctgaaatta caaaatttcc
     6361 tgtatttata aaacctctga atggtagttc atctgttaac acatttattg taaataatga
     6421 aaaagaattg ttgtttttta gtgaatatat agataatcct cttattcaag agtatatttt
     6481 aggtgaagaa tatactatag atgtttgctt aaactttgat tcgacaccag taagtattgt
     6541 tcctagaaaa agaatagcaa ctagaggtgg ggaaatactg aagggtcaga ttattaaaga
     6601 tagagaacta attgagacta cgaaaaagat gttggaaaaa ttaaatgcaa tcggacatat
     6661 tactattcaa tgtataaaaa acgaaaaggg aatattttat ttagaaataa atgcaagata
     6721 cggtggagga gcccccatta gcattaaatc aggagctaat tcccctgaat atattataaa
     6781 gatgatagat tcaaaaaaaa atttgagata cagagaaaat tatttagata aaatgatagc
     6841 actgagatat gatcaagcga tttatttaga tgaaaaagga aatgtatata atgattaaag
     6901 cggtgatctt tgatttagat gacactcttg tgtctgaata tgattatgta aaaagtggat
     6961 acaaatgtgt agctagaaga ttagcagtgc aaaaaatttt cccggctact tttgacgaaa
     7021 tatatagagt ccttttagat ctttttgctg aagatagtaa aaatgtattt aatcgtctat
     7081 atgactattt tgaaattcct tatagtaaaa aagaaattaa agaactggta aatgtatatc
     7141 gagaacacaa accagacata aattttttag aaaatgcaga tactttatta acggaattaa
     7201 gaagtagagg atacaagctg gggattatta cagatgggta tgctataacc caacgaaaca
     7261 aattggatgc tttgaaagta gactatttgg tagattatat ttgtattact gacgaattag
     7321 gccgtgaatt gtggaaacca caccctatct catatctttt attgttagaa aagtttaatt
     7381 taaaggcaca agagtgtata tatattggcg ataatgagtt gaaagatttt ataactgcca
     7441 aatcaatagg aatgcaaaca attaaaatag aaagagtaaa tggtatttac agagaaagca
     7501 gtttgaagtt caaaagattt taccaagctg ataaaaaaat acgttcgtta tgggagataa
     7561 aaaatttttt gggagtgatt tcatgaaaag taaaagaatt ctattagctt ctccacatat
     7621 gagtgaagaa ggatatgaaa aagagtatgt aaaagaagca tttgatacaa attgggttgc
     7681 tcctttaggt gcgaatgtga atggatttga gaaagaatta gcagaatacg tgggagctag
     7741 tcatgctgcc gcattatctt ctggaacagc agctatacat ttagctttga aagcggctgg
     7801 ggtcggtaaa gatgacatag tgatttgtca gtccttgacg ttctcagcta ctgctaatcc
     7861 aattctctat caaggagcta tacctgtatt tgttgatagt gattacgaaa cttggaatat
     7921 gaaacctgaa catttgcgaa cagcactaga aaaatatcct aacacaaagg ctgtattagt
     7981 agtacatttg tacggtttat cagctaattt agacgaaatt actaaaatat gcaaagaata
     8041 cgatgttact ctaattgaag atgctgcaga atcattagga acaacatata aaggaaaata
     8101 tactggtaca tttggcgact atggaattta ttcttttaat ggaaataaga taatcacaac
     8161 ttcaggtggc ggaatgcttg tatccaatga caaagaaaag gtggaaaaag tgagattttg
     8221 ggctacgcaa tctcgtgaga aggcacgcca ttatcaacac aatgaaattg gctataatta
     8281 tcgaatgagc aatatcgttg ctggtatcgg tcgaggacaa ttgaaagtat taaatgaaag
     8341 aattgagaaa aaacgatata ttcatagttt ttataaaaaa gaattgtcta aattgtcaaa
     8401 tatacaattc atgccagaaa ataattggaa tgaaccgaat tattggttaa gtgtcataac
     8461 aataaaggga ataaatgcga atcatcttat tgatgagtta gacaaaaata atatagaagc
     8521 ccgcccagta tggaaaccta tgcatttaca acctgttttt aaaaattatg attatattgg
     8581 cgatgatgta gcagaaactt tatttgactt aggagtatgt ttaccatcag atacaaagat
     8641 gacagatgaa gatttaaaat atgtagtatc tacgattaaa agagtgatca atgatgcgta
     8701 aaaaaacttt ctatcagctt tttgggaaaa gaattttaga tatcctattg tcaggaattg
     8761 ctctcatcgt tttatcgccg ataatcttaa ttgtaggttt tttagtaaga attaaacttg
     8821 gttctccaat tatcttcaaa caagaacgac caggaaaaag tgaaaaaatt ttttccatgt
     8881 ataaatttcg tacaatgact gacgaaagag accacaatgg agaatattta cctgatgaaa
     8941 ttcgactaac taaatttggg aaaatgctta gagcaacaag tctggatgag ctacccgaac
     9001 tttggaatat tttgaaaggt gatatgtcga tcgtaggacc tcgtccatta ttagtggagt
     9061 atttgccact atatagtgag aagcaacgga aaagacacaa cgtaagacca ggattaactg
     9121 gactggctca ggtcaatgga agaaatgcta ttagttggga agaaaagttt gatttagatg
     9181 tttattatgt tgataaaatt agtttcttta atgatcttat tattataatt cagacatgta
     9241 aaaaagtaat caagaaagag aatattaata caatcaataa caatatccca gaaaaattta
     9301 aagggagtta aatacagcag cttaatgcaa gttttgtgac ttgcattata tttaggtata
     9361 aatcaaagcg agtatattag aattatataa catatgttaa aaaaacgtat agctaataaa
     9421 agtatgattt taaaaatttg tgtagagttt taaatatact gtttcaatac gagaaaatta
     9481 taaaaaagta taaggaaaag agagttatgg caaagtatta taataaaaaa attcttataa
     9541 tggcaaacaa cgatgatggt ctttaccgtt ttcgaggaat gctcataaaa aacttagtag
     9601 atcaaggtaa tgaagttatt gtatctcttc caaagggcaa gaatattcct aaattaattg
     9661 aacttggatg tcaaattatt gaaactccaa tagatagaag aggaattaat ccaaaaactg
     9721 actttaaact attttttaac tattgtaata ttttgaaaaa aataaagcca gatatggtca
     9781 ttgcttatac tatcaaacca aatatatacg gtggattggc atgtcggtta agaaaggttt
     9841 cttatgcatt aaatataacc ggattaggaa cggcatttca aaaaaagggg gttttgaaaa
     9901 gtcttattgt atttttgtat agattagcag ctaaaaaatc taaggtcata tttttcgaaa
     9961 attttgaaaa tatgaaaaca tttcagactt tacaaattgc taaacaaagt aaatacaagc
    10021 tattaaacgg tgcaggagta gatgtagaac actttagtta tttagaatat cctaaagatg
    10081 agcaagttca ttttttattt attggaagaa ttatgaaaga gaaaggtgta gatgaacttt
    10141 ttgaagcaat gaaaaactta tatagtgaag agaaaaattg tgtacttgat gtccttgggt
    10201 attttgaaga agattacggc ataaaaattg aaaaatacga aaaagaggga tggttaaaat
    10261 atcatggata tcaatctgat attcgtccct ttgtagaaag agcacattgc tttgttttgc
    10321 ctagttggca cgaaggaatg gcaaatacta accttgaatc agcagcatca gggagaccaa
    10381 ttattacaag tgatatccca gggtgtaaag aagcagttat tgaatcgaag tctggttatt
    10441 tatgtgaaag aaaaaatagt tgtagtctct ataattcgat gaataaattt ctaaaattat
    10501 catatgagca gcgcattaac atgggaaaaa tggggagaaa ttatatgatt aaaaaattta
    10561 ataagaagaa agttgtcgat gaaacaatta aatcattatt tagttaaaaa gatatgtttt
    10621 gcaaagtatg tgatagaaaa gagggattct gtggcttcag aatagatatc aaagaattgg
    10681 caaaagtttc tgatattttt ttatttacta cattacaaga aggaatgcca agatctatga
    10741 tggaagctat ggcaagtgga ttgccatgta tagcaagtaa aatccgaggt aatgtagacc
    10801 ttttagtgga aggaaaaggt ggatatcttg ttccagctaa agatgcagaa ttagtagcag
    10861 agtgtttgag agaactaatc aataataaat ctcttagaaa gtatatggga aatgagaacc
    10921 tttccagaat taaagaattc gaggttagta tcgtaaaaaa aagaatcaaa gaaatatatg
    10981 aagaagttat aggtgagagt gtaaaatgaa aaatccaatg ttttcaataa tagtacctat
    11041 ctattgcgtt gaaaaatatt tgaaagattg cttagattca ataagaaatc agacatttaa
    11101 aaattttgaa gcaatactag ttgatgacgg tagtcctgac gaatgcccag aaatatgtga
    11161 ctattattcc caaatagatg gacgtttaaa agtaattcat aagaaaaacg gtggaataat
    11221 agatgcacgg aaaacaggag tcactagtgc tatgggagag tatatagttt ttctagatgg
    11281 tgatgattgg cttgcaaata cttatttaga aaaatatgcg gaagtaatta ctaagtacaa
    11341 tccagatata atatgtagtg ggtattttgc atcacgttcc aacaaaatta gaaaaagaga
    11401 attgtcttgc tcgtttggat attatgacaa agataaaatt ataaatttga tttatcctaa
    11461 attaattgag gatgaacatg gagagtattt tagaccacaa ttatgggcaa aagcttttag
    11521 aactactctg tacaaggaac aacaacaagt atctgggcaa gttagtgttg gtgaagatca
    11581 tgcatgtaca aaaccatgta tttaccatgc aaattcgata tatatgatgg aaa
//
LOCUS       DACCYH010000095        11536 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.099, whole genome shotgun sequence.
ACCESSION   DACCYH010000095 DACCYH010000000
VERSION     DACCYH010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.099"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            310..516
                     /locus_tag="IVY85_002400"
     CDS             310..516
                     /locus_tag="IVY85_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP8045399.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(718..1719)
                     /locus_tag="IVY85_002401"
     CDS             complement(718..1719)
                     /locus_tag="IVY85_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8045400.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1724..3637)
                     /locus_tag="IVY85_002402"
     CDS             complement(1724..3637)
                     /locus_tag="IVY85_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8045401.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3805..4311)
                     /locus_tag="IVY85_002403"
     CDS             complement(3805..4311)
                     /locus_tag="IVY85_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8045402.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4471..4911
                     /locus_tag="IVY85_002404"
     CDS             4471..4911
                     /locus_tag="IVY85_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8045403.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4937..6094)
                     /locus_tag="IVY85_002405"
     CDS             complement(4937..6094)
                     /locus_tag="IVY85_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045404.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6097..6453)
                     /locus_tag="IVY85_002406"
     CDS             complement(6097..6453)
                     /locus_tag="IVY85_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045405.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6751..7725)
                     /gene="sgrA"
                     /locus_tag="IVY85_002407"
     CDS             complement(6751..7725)
                     /gene="sgrA"
                     /locus_tag="IVY85_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP8045406.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7921..8760)
                     /locus_tag="IVY85_002408"
     CDS             complement(7921..8760)
                     /locus_tag="IVY85_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8045407.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8945..9832
                     /locus_tag="IVY85_002409"
     CDS             8945..9832
                     /locus_tag="IVY85_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP8045408.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10426..11120)
                     /locus_tag="IVY85_002410"
                     /pseudo
     CDS             complement(10426..11120)
                     /locus_tag="IVY85_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(11104..11502)
                     /locus_tag="IVY85_002411"
     CDS             complement(11104..11502)
                     /locus_tag="IVY85_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8045409.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3440 a         2301 c         1726 g         4069 t
ORIGIN      
        1 attttatttc acagttgata tgattgttat gtaaaataaa aaagtttagg atacatcgaa
       61 tttttgggga aattcttttg ggggtatcct aaatcttttc tattatattc ccatattttc
      121 tattattggt ctagacgtat gatggtaaac tttgtaccta tctgattgct gaatcattaa
      181 ttgcttatca ttagccatct ttgtttcaag ttcgctagat gatcatttta ttcacatcct
      241 aacaaacttg tcacatttat agattcttaa tctatttatc atacaattat aaaaaaggag
      301 gtgctgctta tgaaagctat ccatatttct ttgctttttt tcgctagtgc attgatttta
      361 tttgctattc atctttgttc ccagccttat atcaataaca acggaatatt agtggaacca
      421 gcattctttt gcttaccact cggctttttg tcccttcttg cctccgcagg cttcggattt
      481 gttgcttttt ttaaacgatc aaaacaacaa atctgactta taaaatttac gtaaaagaga
      541 tcgtgacaat aatcgtgtga tattcccaaa tttcgaataa aaggtgcccc ttgccatctc
      601 taactcctca gtcacaagtt acaatattca taaaccatga aaagcttgtt tattgaaatt
      661 gctccttgtg acttggagct aagtgacaga ttacaacctc ttttgcatca taagacttta
      721 ctccttgaaa aacattacta acttttgata tgtttcttgc gcttgtttat tgctgaaatc
      781 aaactgatat tcatggttca aaacagcatt tttctccgca taatctactt ttgtgacttt
      841 cacccccgct ttctcaaata cagaagttac ttcttccatt tgtttcgtaa acgtatttgt
      901 atttccatcc gttaagaatg tgttttgcgt ccaacgatcc gcatgtctga ccaaggctaa
      961 acttttgacc ttctctgatt gttcaaagtc cttattatca aagtaagccc atccacattt
     1021 ttcataaaga aagctagaga ttttatcact tacttgagta agtgcagaag gatccaacag
     1081 accagacaaa gaaataaact tcgtaatctg cttgttctcc aaagtcggat caagatgatc
     1141 agctttttta atttcttcat tttgaagtgc taacaaatat tctccactaa tttgtgcacc
     1201 tgccgaattt ccaccaatcg aaatattcga ccaatctgct tgatctttta tctctttttt
     1261 agctgcttga atggcttcgt tcaattggat aactggtgta ggatgttttg cttccggtga
     1321 tcgttcataa tctatattta cgatgatttg ctgcgtgtct gaacatagat attccaaata
     1381 atcacgacaa tcatttttat cccctcctac atatgcgcct ccatgcatcc agaaaagaat
     1441 cggtaatttt ttcttttcca ctttcggtgt gtagatatcc atattattat ttttgtactt
     1501 tgaaggatag gatatttctt tttttatatc gatttgttcg attttttgct ggaaatcatc
     1561 tggtcgggca tatacttgtt ctttttcact tgtgtcaaat tgttttctaa caagcaagct
     1621 aatcggcttt ggtgtgattt gattaatcaa aaatacagtg actaaaataa ctacgatgat
     1681 aactggtact gcaatcacta ttcgcttgaa ccatttcatt catttaccct ctctttcttc
     1741 gccaaatcca tgtgataatc gtgctaacag ataaaaatcc acctgaataa atgagaagcg
     1801 aatgtttctt ttctccagtt ttcggcaaca ttcttttttt tgcatctgtc tttttttgta
     1861 ctttcactgt catctgaatc ggttgcgtat catcacttga ttgatcctct cgaggttcat
     1921 tctttccctt attgtctgca ttctctgtcg gatcttgctt ttttggtgga gctactggga
     1981 tttttttgta atagaaaata atagttggta tcgtctcatt aaaaatccct tcagtcggtc
     2041 cttcaacatt tgctaattcg tatccttcga atgtcttttg ctctgtcata taattttctc
     2101 cataagctcc gtaaagctgg atttcgtcct ggtctttgag tagactttta ttgttttcat
     2161 ccacatactt tacgtttatc tgatcatgga acactacttt caaaaagtcc gttacatgtt
     2221 tcgtgtaggc agcatggtca acttcctgtg cctttccatg ccctgctcct tctactaata
     2281 aaagatcttt ataccctcct gataactcat ataatttttg cgcgttagac acaggtacta
     2341 catcatctgc tgttccatgg ataatcaatt taggcaatga atctgacatt ccaatcgtag
     2401 gtagagcagc ttttgtatcc aaatgcatct cctgatcaaa atagtattgt ttcaaaaggc
     2461 ccataaataa atcttcatgc tcttgatccc caatgatgtc taactgattt tttatggcat
     2521 ccggtagtgc gggggctact gtctggctgt acgtaaaacg tagttgttca tcaattgatg
     2581 caaatccgca attttcaata attcctttga cattgacagg aatatctttc gctaatacag
     2641 acatcatcgt agctgctccc atcgaaccac cataaagtaa aatcttttga gatggccgct
     2701 ctcgaacttc ttgatttatc cagtaaagga cgtcatctga ctcatattgt ccataggtca
     2761 cataatcgcc tccgctatta ccagtcgctc tggaatcaac aaataataaa ttatacccgg
     2821 ccttataaaa atcattgtat tcttctgtaa cgatcccgtt attccaattc cctcgaaatc
     2881 cgccatggat catgactgtt ttatctgaac cttgatcgac ataatatgca tatacatcca
     2941 tcattttgcc atcttgtttg tttagtacag tttgatgaag ttgttttgta tttggataga
     3001 agcctgtgtt ttctgtaggc gttgtcccat catctgtacc tccaagcatc gacatcaaca
     3061 cactgttttt tgcaaaagcg atatcgtaca atagataagt agcaatcaga tgtttgataa
     3121 tctctttatc tacaccataa tcattaacag caagctcagt gactaataac tctattaatt
     3181 ttgacttgat gtcttctgtt ggtccattaa gtatttctgt catctgttta ccgaagtaat
     3241 tcaaatattc tttaccattg aaattttcac ctttaccgat atcatcattt tctgtcccat
     3301 tctctttgtc acttacttta gcagaagagg attcgtcgtt tgacattttt gtagctgttg
     3361 cttcttctgt ttgttggtta tctgtggtag cggtcgtttc ttgatctgtg ctagtaacag
     3421 atgtctcaac cgttgtttca gtatttgttt ctgtttgtga agtactcgat ttcgtttcat
     3481 ttccagcatc agtagtttct gtggtttcta cctcccgaga ttccgattcg gaagaaatgc
     3541 tctctccata tactgtccat tgcgggacgc ttgtacttac agcaaataag atgaccaacg
     3601 ctccctttaa tacttgtttt gttctcaagc tattcactcc tttttatgtt tctaggagaa
     3661 ttatatgtaa acgcaaacat tttttataca aaaaataaat ggtttatccc ataaaaatag
     3721 aacaggatta ttttctcatt ttacgtaata aagcagaatg gataaaccag tatccattct
     3781 gcttttttta aacgaagaaa caacttattg ctccaaaaat gtcagaaatc gtttatccca
     3841 aattttctcg taaaaagcga ttgtttcctt tgctttcata aaaggattat cataagttgt
     3901 cgttgctcct ctacacatca taagctggta tcctaatcca tgagcaaact tcacagtcgt
     3961 atctatacaa tactctgctt gtgccccaca taattcgatc gaatgacagt ccaattcagt
     4021 taataaaaca ctcaaattag tcttataaaa tgaattcgca tgggtttttc tcacaaaata
     4081 actgtgctca ggaatagata attccgacaa tagctcccat ggttttgttt gtggcactaa
     4141 ttgagcatct tcgtgttgaa cgaaaataac tggtttccca atcgtttcgt atgttttgat
     4201 tcttttattc actaatgctg tcactgtttc taattcgtgt atcggcgaag aatcaaaaca
     4261 tacccctttt tgtaaatcga tcactactat tgcgtcggct gtgtttgaca tacttgtgcc
     4321 ttctttcttt tttcttgatt taatagtatt tcttatctag ctagttccat tatacgtgac
     4381 aacattttct tattttacac ctctaaatgg cacttttcga ctatttcttc atgttattat
     4441 ttcataaata cttagctatt ggaggaatgt atgatgatta aattgtatac aggttctggc
     4501 tgctcgtctt caagacgcgc aaaacgttgg cttatcgtcc atgatatacc gtttatcgaa
     4561 cagaacttaa ttcgtcaacc actaaccaaa aatgaattta tccacttact aaaattgaca
     4621 aacaacggcg tgaccgaaat gctctctact cattctatta gttataaaaa tcttgttcac
     4681 agaatagatg aactcagttt gagtgaacta tatattgaga ttcgtacatc cccttcttta
     4741 ttgaaaaaac cgattatcta cgaccaatct gcaaaacttt tgactggatt tagcgaagat
     4801 aaaatcagga tgttcctttc taaaactaaa agaaagcaag agctcaacga actcttgctt
     4861 tctggtcatc ttgtcgattt taaagaaaat aatctttttg caatgtctta attttggcat
     4921 aaacaatctg cttttcttaa ttaacgaaat ccaatttttc caatatggat ctatttttct
     4981 cattcctgat ttttatgatt tcagctttca gacgcgccca atcatttttt gtaggttgtc
     5041 cattccagta aaatatctgt gtattcgttt gatcatatgc ctttttgtcg ataggataat
     5101 cgctaatgac aatatcaggt aactgatcag cggatgcact atattttatt gggcgttcag
     5161 tcatagataa tatccttttc tttgcccata attcctgtgt tttaccatac tttgattgaa
     5221 tatagaatat gacaggttgt tcgtgtttta taaaaatatc cctgactaac atacagtatt
     5281 gaaaaatcaa ccgtctgttc ttttcataat aagtattaag ctctggaatt ctgcgagcta
     5341 ccccactaaa aaactgttgc atgattggat acatcactgg aaatatatgc tgaaaatctc
     5401 tgtcttctgc tgattttcca aaagcatcca ccttcttttt tgtcccaaaa acccttgaat
     5461 acaaatggat cattgccaaa ttgacatata gataattttt ttctgaagtt gtaaaacaaa
     5521 tcttgaagtt tttttcaaaa ttttctacaa tagacgattc aatccagccc caactttctt
     5581 gtactttatc tggagtatcc aacaaatctt cttccaaata ggtgttcatt acaccaaaca
     5641 taaaataaag gtaagcttgc tctgttggag tgatgtgatt catcgaccag aagtttgctt
     5701 caaaaaaagg ctctagtatg taatgcgtaa actcttcttt tgttatcaat ggatgcacaa
     5761 atttgttcat ctcttctggg acatacgtaa tcgagtggcc ttgtttcatt cgttgcaacg
     5821 aaattcttag taggctgatc catttctttc tatctatact acgcgtatat ggcgcatgtt
     5881 tcgcaaattt ttctaatagt tgacgtttat tttttatact tgataatata tcagaatgat
     5941 tttcgtcgaa tgactgccaa tacatcaagt gaaaaaagta tcggatacgg tattcttctc
     6001 cttctatcgg attacctttt ttcaagctgc aagataaatg atagtttttc aaaatagtat
     6061 taagttctct taattttctt tgaatcgtac gcaaacttat ataatttttt gactaagatt
     6121 ttctaaggat tgataagtac ctttgtaaag tgaatccagt aaaatataac caatcgttcc
     6181 ctctaaaagt atagaataaa ctttactcag taaaaactga gaagaaaact cgacttttat
     6241 catcttgttt tctgaaagaa aagataattt atattctgga tacctttcgc aaaacgattc
     6301 tgcttgttga atcaccgaat tcatcttagc cagggtgtag tttagatcta aagcgatatc
     6361 cttcaccaca tataatttct cgtctctttc ataacaatag ttcaaaaatt gatatacttg
     6421 cctttcattg atatcaataa attcttcaaa caaagtacat actccttttt atctattatt
     6481 tagacttatt ccgacagcta catgaataac gtacaaatag attcgttact gatttttcag
     6541 agatactacc atgcgcagtg tgcattgtca attattcttg ctcttatttt ccatttgtgt
     6601 tcctgttatt tcctgtttct caaatatgtt agtaaacaaa aatagatctg gcaaagctac
     6661 aacttcgcca ggcaatacag cactctcgaa tgaaatattc actcaaaaat ctatcgtttt
     6721 attcaatttt agatctgttg cttttattct ttatttcact cgatggaaga gagaacgaaa
     6781 atagcttgct cctacaatca aaatgagtaa cacacccatc catgtaatta gcttgttcaa
     6841 ggttctacta ccagtttctg gtaaatcgct ttttctatta tctgtttctt tcgctttttc
     6901 ttgatgatca attccacgag aaacaaaatc aggctgtttt agcacacttt ttaggttgct
     6961 ccctgcattt ctcttttgtt ccttagttgg tatgataggt accaaatcaa gttcattatt
     7021 tacttttcct gtgctgcttt cgctgctcgt gcttcctgtg ctactctcac tactcatgct
     7081 tcctgtgcta ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgcttcctgt
     7141 gctactttca ctactcgtac tctccgtact gctttcgctg ctcgtgtttt ctgtgctaat
     7201 ttcactgctc gtactctctg tactgctttc accgctcgtg ttttctgtac tgctttcact
     7261 acttgtgcta tccgtactgc ttggcggtcc aacaactgtc caactgcttg gtttcaaatt
     7321 ctccgtgatc gtcatctgtc catcataaaa aacaatcaaa tcaactttat ctttactctt
     7381 gatctcatca atcgatagtt ctgcagaaat cattattgta tactcaatac cagcggtcca
     7441 ttgtacgtct tttaaataaa cttccccaat aactttttct gaacttccat taccatccca
     7501 accatatgtt agaacagcat gtacatcact agcattcacg cttttgtttg gtttgactgg
     7561 aatagtaatg acttgttgtg tcatgtcata aacaatccga tcagttttca cagtaaatga
     7621 aagattctca tttgcccgtt catttgcaga agtactgatt ccaatcattg ctgcaattat
     7681 actcagccca agaataacaa ataatacggt cgctgttttc ttcatttcta tcactctcca
     7741 tataattacc ttctatttga taaaggaatt atataaaatg aaaatgatga gaacacgaca
     7801 gtcagttgtg aaaatctaac aaaagatgtc gtgtattcgc ttgaatttgt ttactttttc
     7861 gtttaatcaa tgacagactt aaaaaagcaa gaccgatatc agccttgctt tcttcatctt
     7921 ttattgataa acagcagtgt atttcttcat ccgctttgtt ttatatgcat agagtaacaa
     7981 accaacaatc aagactaaca agaataatgt agttgatata gatgcttcag ccccaaagtt
     8041 tcccccagta attattgtat tttgcgattg gatcagagtg gagaaaaaac cttgttcttg
     8101 cgttccgctg actggtatac ccaaaatagc tccttgtgtc caattccaag taatatgagc
     8161 accaatagcc gtaaataaac ttcctgtact gtagataagt gcgctgaaaa agcaacctgc
     8221 taaaaagata ttgaaaactg ccagaaatgt catgttagga ttcaatccgt gcaaaagagc
     8281 aaataatgca gcagatccaa tcattgctat taacggacca aatctaagca tctgatgctg
     8341 aaaaaagcca cggaataaaa gctcttctga tgcccccact gcaataaata aaataaaact
     8401 cgttatcagc ggcacaatgt ttgcgtttcc tgaatctaac cgatacgccc ccgtcaaata
     8461 taatggacta gtaataagca gaaacactcc aattccaaca acgagacctc ccgctaaata
     8521 cccccagtct tttcctcgaa cagaaacacc ataatcttct attctttttc cttcgacctt
     8581 actagcaaga aataaagcta gtaaaaaaac agctaaatgt ccgaccgtac cggcaattgc
     8641 tccaatccat tcactatttc caataaacat atccgcaagt gcattattga caagaattcc
     8701 taaaacaaaa ccaacaaaga ctattaaaac agatccaatc aaccgaataa tcctgatcat
     8761 ctttcatccc tcctttatat taagtttaac aacccttttc tttcttgtct cgtgaaaact
     8821 atgtaaatgt tctccatcat agagagaata gatttttacg aactaagaat atctacagca
     8881 accttacgaa aatgaaagac tgtctcccta attcttctgt tagactgttt ttaataagga
     8941 aaggatggga aattttttga aaaaacaact actaattatg tgcctttcag catgtgcaat
     9001 tttactattc tcaggttgta gcaagtccaa tgaaacgatt gcaacgttta aaggtggaac
     9061 acttacagtt caggattttt atgataaccc aaaagttaaa tcaaataatc aaacactatt
     9121 aaaaaaaatg attgtcttca aagcatttga tgatgtatac ggaaaagaga tttcttcaaa
     9181 ggaagtaaca aaagaatata atgaacaaat caaaaaactc ggtccaaact acaaggaaca
     9241 attaaaagca gttggccaaa cagaagagac atataagaaa ctctttaaac agatgctggc
     9301 tttccaatac ggtttgaaag ccaatgtaaa attaacagat aaagatctag acactgcttg
     9361 gaaagaattc tatccagaag ttagtacaca gatcattcta ttttcgacta aagaagaagc
     9421 agataaagca aaaaaagaag caaatgaagg agaaaatttc tccaagcttg ttcaagctta
     9481 tggaaaaaat aaaacattaa aggaaactga tggaaaaatg aattttgatt caaccaatcc
     9541 agagattcct actgaagtaa aaaaagcagc atttaaacta aaaaacgggg aagtttctga
     9601 tatcattcct gtaacagatc caacgactta tcagcaaagc tattatctag taaaaatggt
     9661 aaaaaaacag gataaaggtt ctaataaaga caaatacaaa tctgagctag aaaaaattgc
     9721 taccgaagca aaaactcttg atacggaatt catggataaa acaatccgta aagtgatgaa
     9781 aaaagacaat gtaactatta aagatcctta tgtaaaaaat atattcaaat aaacaaattt
     9841 gacccaattg gggatgtggc aaaatttgag aagtgatttt tggaaattcc ttcttatttt
     9901 tgaggcaaac cacttttgcc acaacttcat cggggccttt ttttatttgt accaaaacaa
     9961 atcactggat ttgttacaat catgaggata taatttaaaa aagtttttta tttctatcac
    10021 atgaatcaat gcgccattaa gtagattctt tcatgcttcc ctattttttc acttgccgta
    10081 tcgcttcaaa aacaactatc ataaactcaa actcgactga aaagttattt taaaaaagac
    10141 taaaaatatc ccttctggac ttgttcttct tattctgtta tactagagaa acacgaaaga
    10201 tatttttatg cgacactttt catttgtttt tatgaaattt attcaaaact ggcgtaactt
    10261 cagagttaca aggatgtatt agaggttagg gaatacatcg tttaaatgag aaataagtaa
    10321 tagttagtaa tagtcaccca aactttaaat taatgaatat tgaatcgtca gatagcctcc
    10381 tcgagttttc gagaaggcta tcgtttattt tatggagaaa ttgattcaga agtaatcagc
    10441 gacctttttt agttcaccaa tcgtttctgc ttggaaagtt tgcacccaat cttcttggaa
    10501 gcccctcact gctttatcaa tcgagggcat ctctaatact tgatctagta gctctttacc
    10561 taacgttatc gctgagctcc agatcgaatg gctgtagtga cttgttcaca atttttgaaa
    10621 ctagctgcta atatttttgt tggtctttgc gtacgttcga tctcagtccg caacgaatca
    10681 atcactttca tagcatcaat gccttgattt tgcatcctgt tataataagg tgcaatataa
    10741 tctgctccat tattaatagc taaataccct tgtaattctg tgtaaatagc tgtaccggta
    10801 atcaaataac cttctttttt caactttttg atcgcttgaa ggcctgcttg attcaccggc
    10861 accttgataa acgtatcttt tcccagttct tctatgataa ctcgagcatc ctctatcatt
    10921 tcttcttccg ttcttcctat tgtctgaata tggataggaa tctgtccgat tatttttttg
    10981 atttctttca tatgcgagaa gaagtttgac gctccttctt ttttcagaat tgtaggattt
    11041 gttgttacgc ctgttaaatt caaaaactgt tgacaagaat aaatactttt tgtatttgct
    11101 gtatcaagca tgatttccat atagctttcc cccattctga tcctcttgat cgattttaat
    11161 gattgtcccc tttcttaaat gcggcatttc cttatcttct acatacattg ttcctggaag
    11221 ttccggctca tgagatccat caaattttat cgtgatatgt cctaattcat tcaaattagt
    11281 ttggacaaca tctcctactg ctgtgattac atacatttgt ttatcaaaac agagcttcat
    11341 atcacaagtt atttcttttc ttacctgatt caattcaatc ttataacaat aatctgttaa
    11401 tgtttccggt gcttcatctc caaataaaat cattattttt tctttcctga atacttctgc
    11461 ttctatacca acatctgtta caaccgtttc aaagatcgtc atctctgttc ctcctcgtaa
    11521 aatatcattt gtctaa
//
LOCUS       DACCYH010000096        11300 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.100, whole genome shotgun sequence.
ACCESSION   DACCYH010000096 DACCYH010000000
VERSION     DACCYH010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.100"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            12..84
                     /locus_tag="IVY85_002412"
     tRNA            12..84
                     /locus_tag="IVY85_002412"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="IVY85_002413"
     tRNA            88..160
                     /locus_tag="IVY85_002413"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="IVY85_002414"
     tRNA            168..249
                     /locus_tag="IVY85_002414"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="IVY85_002415"
     tRNA            267..339
                     /locus_tag="IVY85_002415"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="IVY85_002416"
     tRNA            351..422
                     /locus_tag="IVY85_002416"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="IVY85_002417"
     tRNA            439..524
                     /locus_tag="IVY85_002417"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="IVY85_002418"
     tRNA            535..608
                     /locus_tag="IVY85_002418"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="IVY85_002419"
     tRNA            618..691
                     /locus_tag="IVY85_002419"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="IVY85_002420"
     tRNA            711..783
                     /locus_tag="IVY85_002420"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="IVY85_002421"
     tRNA            811..884
                     /locus_tag="IVY85_002421"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="IVY85_002422"
     tRNA            898..971
                     /locus_tag="IVY85_002422"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="IVY85_002423"
     tRNA            995..1084
                     /locus_tag="IVY85_002423"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="IVY85_002424"
     tRNA            1097..1170
                     /locus_tag="IVY85_002424"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="IVY85_002425"
     tRNA            1189..1264
                     /locus_tag="IVY85_002425"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="IVY85_002426"
     tRNA            1266..1336
                     /locus_tag="IVY85_002426"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="IVY85_002427"
     tRNA            1350..1423
                     /locus_tag="IVY85_002427"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="IVY85_002428"
     tRNA            1448..1535
                     /locus_tag="IVY85_002428"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="IVY85_002429"
     tRNA            1557..1628
                     /locus_tag="IVY85_002429"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="IVY85_002430"
     tRNA            1651..1740
                     /locus_tag="IVY85_002430"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="IVY85_002431"
     tRNA            1753..1826
                     /locus_tag="IVY85_002431"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="IVY85_002432"
     tRNA            1845..1917
                     /locus_tag="IVY85_002432"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="IVY85_002433"
     tRNA            1921..1993
                     /locus_tag="IVY85_002433"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="IVY85_002434"
     tRNA            1998..2068
                     /locus_tag="IVY85_002434"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="IVY85_002435"
     tRNA            2081..2154
                     /locus_tag="IVY85_002435"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="IVY85_002436"
     tRNA            2166..2237
                     /locus_tag="IVY85_002436"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     gene            2503..3696
                     /locus_tag="IVY85_002437"
     CDS             2503..3696
                     /locus_tag="IVY85_002437"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP8045410.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="IVY85_002438"
     CDS             3826..5298
                     /locus_tag="IVY85_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8045411.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="IVY85_002439"
     CDS             5381..6169
                     /locus_tag="IVY85_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP8045412.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="IVY85_002440"
     CDS             complement(6236..6904)
                     /locus_tag="IVY85_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8045413.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="IVY85_002441"
     CDS             7019..8731
                     /locus_tag="IVY85_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8045414.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="IVY85_002442"
     CDS             8734..10506
                     /locus_tag="IVY85_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8045415.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(10588..>11300)
                     /locus_tag="IVY85_002443"
     CDS             complement(10588..>11300)
                     /locus_tag="IVY85_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8045416.1"
                     /translation="TGMIFKFANRRYGLKYNPVNGVSLARKPKTNEEIKKEKDGYLSK
                     EEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELISLTESDYDGNKITVSGTYDYE
                     LKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIKNNKDKYIFISKNGNPISIQAY
                     NQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTH
                     TTKNMQNQLVEKLGKIEI"
BASE COUNT         3252 a         2024 c         2555 g         3469 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggcagaaaag gggcaattta aatttctatt tttcctagtt tttctactag
    10621 ttgattttgc atatttttag ttgtatgagt ataaatcttt aatgttgttt ttgaatcttc
    10681 atgtcctaca cgatccataa tggcttttaa tgggattcct aattctgtaa gtagagaaat
    10741 gtgggaatgc cttaacatgt gagaagaaac ttttttatct aggtttaatt ctttggatac
    10801 tgctcttaaa gattgattat atgcttgaat agatatcggg ttaccatttt tagagatgaa
    10861 aatgtattta tctttattat tcttaattaa tttgttttct tcaattaatt gatctatgat
    10921 ttctttagca ttacttgata attcaatggt tctataagaa cctgtatttt tagttgttgt
    10981 tttaattttt aactcgtaat cataagtacc agaaaccgta attttattac catcataatc
    11041 agattcagtc aaagatatca attcaccata acgtagtcca gttaaaaaca aaaatttagt
    11101 aattaaagaa taacgtgact gttgatgtga acttttttgt ttactagcaa tcatttgaac
    11161 ttcttctttt gataaataac catctttttc tttttttatt tcttcgtttg tcttaggttt
    11221 tcttgctaaa gaaaccccat ttacgggatt atattttaat ccatatcgac ggttggcgaa
    11281 tttaaaaatc attcctgtaa
//
LOCUS       DACCYH010000097        11130 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.101, whole genome shotgun sequence.
ACCESSION   DACCYH010000097 DACCYH010000000
VERSION     DACCYH010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.101"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            92..1351
                     /gene="lysA"
                     /locus_tag="IVY85_002444"
     CDS             92..1351
                     /gene="lysA"
                     /locus_tag="IVY85_002444"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP8045417.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1338..2138
                     /gene="dapF"
                     /locus_tag="IVY85_002445"
     CDS             1338..2138
                     /gene="dapF"
                     /locus_tag="IVY85_002445"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP8045418.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2141..3022
                     /locus_tag="IVY85_002446"
     CDS             2141..3022
                     /locus_tag="IVY85_002446"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP8045419.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3019..3735
                     /locus_tag="IVY85_002447"
     CDS             3019..3735
                     /locus_tag="IVY85_002447"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP8045420.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3741..4436
                     /gene="dapD"
                     /locus_tag="IVY85_002448"
     CDS             3741..4436
                     /gene="dapD"
                     /locus_tag="IVY85_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP8045421.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4452..5453
                     /locus_tag="IVY85_002449"
     CDS             4452..5453
                     /locus_tag="IVY85_002449"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP8045422.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5450..6811
                     /locus_tag="IVY85_002450"
     CDS             5450..6811
                     /locus_tag="IVY85_002450"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP8045423.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6771..7922
                     /locus_tag="IVY85_002451"
     CDS             6771..7922
                     /locus_tag="IVY85_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP8045424.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7897..9066
                     /locus_tag="IVY85_002452"
     CDS             7897..9066
                     /locus_tag="IVY85_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8045425.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9241..10620
                     /locus_tag="IVY85_002453"
     CDS             9241..10620
                     /locus_tag="IVY85_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8045426.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10901..11130
                     /locus_tag="IVY85_002454"
                     /pseudo
     CDS             10901..11130
                     /locus_tag="IVY85_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3703 a         1945 c         2494 g         2988 t
ORIGIN      
        1 attttgttta atattattcc aataaaattc aaatctttat caaatgaaat tccaataaaa
       61 acggaaaaaa agtgaaaaga ggaatgaaaa aatgacagaa ttgataattg gcggagtcac
      121 agcaagcaca ttggcagaaa agtataagac acctctgtat gtgtatgatg aagaaaagat
      181 gagacaaacg atgctgtcat tcaagcgagg ctttgtatcg aaacgttttg atacaaaagt
      241 gctttacgcc tctaaagcat tccaaacgat tgagatgctg catttagttc gtgaatatgg
      301 attcgggcta gatgttgtca gtggtgggga aatctatacc gctttgcaag ctgattttcc
      361 tagagatact atctacttcc atggtaacaa caagacgcca gaggaactga tctatgcact
      421 tgaaaatgat ttgttgcata ttgttgccga taatttgatg gaagttgaat tattagcaga
      481 attaagccaa cgctatttaa aaaaaatctg tatcatgctt cggttgaatg tcggagtaga
      541 agcacatact catgaatata tcgtcactgc gcatattgat tcaaaattcg gcatgagcta
      601 tgaaagtaaa gagtgccaac aagcccttca gttgataaaa gaaagcagct atctagagtt
      661 agaaggattt catgctcata tcgggtcaca aatttttgat gttactgctt ggttagctga
      721 aatcgacaaa ttgacgaatt acttaaccga ttttgaccaa cctctttcct tgaatcttgg
      781 cggagggttt gggattcgtt atacacaaaa tgatcagccg atcccagtcg aagaggcact
      841 aaaaaaactg attgaatata cggaagaagc tttggaaaaa cgaaaactga ccattcgtca
      901 attactgatc gaaccgggta gaagtattgt tggagaagct ggtacaacac tatacacgat
      961 tggtttcatc aaaaagacac cacataaaaa atattacttt gtagatggcg ggatgacaga
     1021 taatatccgt ccagctctgt atcaggcaga atacgattgt gatttagcag caaaactgtc
     1081 ggaagaaaaa acagagaaag tgacgattgc aggaaaaatg tgtgaatcag gcgatgtggt
     1141 aattaaagaa gcttatttgc cgaaagcaga atcgggtgat cttttagctg tttattccac
     1201 gggagcttat ggatactcga tgagcagcaa ctacaatcga gcgacacgac cagcagttgt
     1261 ttttgtcaat aatggccaat caagactgat tgttcgcaga caagcatttt ctgatctact
     1321 aaggggggaa gtttcctatg atccttcata aatataatgg ctgcggaaat gattttatct
     1381 tattagatta cgcagaagac acagattata gtcaattagc cgcttcttta tgcacaaaag
     1441 aaaaatatga tatagacgga ttgatcgccg taaaaatcga tccgttggaa atgatctatt
     1501 acaacaaaga tggcagccgc gcgccgatgt gcggaaatgg cattcgctgt tttgctcgat
     1561 acgtaaatga tcagaacatg atgaatgaac aggaattcaa cgtagagaca ttagcaggca
     1621 caagaaaaat tacggtgttg gatccctcac ctttttattg cagcgtagat atgggagtgc
     1681 cagatgattc tccggaaaaa atcaaagcta atcaaactta tccaataaaa aaccaggaaa
     1741 tagagatcaa tggtgaaaaa gtaacgatca ctagtttatt tttaggaacg attcataccg
     1801 ttgtttttgt tgaaaatgcg cgtgaggaag tctggagaaa aagaggtgaa ctgatctgtc
     1861 atcatccttt gttcaaagaa aaaaccaatg tcaactttgt agaagtagtc aatgaacaag
     1921 aattgatcgt ccgtacctat gaaagaggag taggttggac acttgcttgc ggtacaggtt
     1981 gctgtgcctc ttatgtggtt gctaaggaaa ctgggaaaat ttcagcagat caggtagttg
     2041 ttcacttgga acaaggagac ttgatgatct ctggtgagga tgcgatacaa atggctggcc
     2101 cagcagttca tgaatggaca agaatattgg aggaatgaag atgataaaag gttcgatcgt
     2161 ggcgctgatc acgccgatga atgaagaggg atcagtagat tatgctggtt tggaaaaatt
     2221 gatccaattc catttagatg aacaaacaga tggattatta gttttaggta caacaggaga
     2281 atcttctacg ctcacacaat ctgaggagga gcagatcctt caactgacag taaagaaagt
     2341 ggctggccgt gtgccggtga ttgcaggagc tggaacgaat aatacgaaag aaacgattga
     2401 aaaagcgaag cattttgctt ctttgggggc agacgcactt ttggtcatca ccccttacta
     2461 caacaaaaca agtgatgcag gactagctgc tcattttaca gcaattgcag aagcctcacc
     2521 gattccgctt attttgtata atgttccttc tagaacaggc atgtcgattc caatccatgt
     2581 attagtaaat ttagcagaac atcctaatat cattggtttg aaagaagcct caggagatat
     2641 ggcttatgtg atggatgccg ctcggttgat tggagaagaa ttcttccttt acagtggaaa
     2701 tgatgatttg atcctaccag taatgagtgt aggaggaagc ggtgtaataa gtgtatgggc
     2761 gaatatccaa cctaaaatag tccatgaatt agtaaaagat acgcaggacg gaagatggca
     2821 gcaagcaaaa gaaaagcaat taaatgcact tgaactgatc catgccttgt tcagtgagac
     2881 caatcctatt cctgtcaaag cagccatgtc attattagac ttaccttccg gacctctacg
     2941 cctgccttta gtttctcttt caaaagagaa aaagaaacaa ttagctcagc ttttgttaaa
     3001 agaaaggacg gttgaaaaat gaaaatcgga ctgattggca gtggacagat gggaaataga
     3061 gtcgaagaag ttgcacaaga aagaggagat acggtccttc ttttacggac ggcacctaaa
     3121 gaagcaacta cgcttgcttt tacggaactg ccagaactgc ttattgattt ttctcatcca
     3181 gataatttag agaaaatcct ttcgtatagc ttatcatatc aacttccgtt agtcattggc
     3241 acgacaggtt atacacaatc acagttccaa gaaataaaag aacatgccaa acatgtacct
     3301 gtgatgtata gtgctaattt ttcgttcggc atcatggtga tgaatcaact aatcaagcaa
     3361 gcagctgcaa tgttgcaagg ctggcagatc gaactgattg aaaaacatca tagtcgtaaa
     3421 aaagatgcac catctggtac tgcaaatatg ctgcttcaaa caatccaaga agtgcaaaag
     3481 ctgcagcccg tatacaactg gcaagggaaa aaaagagaag aaaaccaaat tggtgtacac
     3541 agtattcgtg caggttcttt acctggtgaa catgatgtat tgtttgctgc tactgatgaa
     3601 atatttacga tcaaacatga atctttttct aatcgaatct ttgctgcagg cgcagtagaa
     3661 gcagcggact ggttgaaaga tcagtcacca ggttattaca aattagaaga tttattaatg
     3721 gacaaagggg tgtaaaaaac gtggaagctc aagaaatcat ccgctacatt caaacggcat
     3781 ctaaaaagac gcctgtgaaa gtctatctga atgtaaccga gccaatcgaa tttaaagaaa
     3841 gtaaagtttt tggtgaagga agcagcttcg ttgtttttgg tgactatgag acgattgctc
     3901 cggttttaga agctgaatca gaaaaaatca ttgaaatcga aatcgaaact gctgcaagat
     3961 acagcgcagt tcctttgcta gatattaaaa aaattaatgc tcgtatcgaa ccaggtgcta
     4021 ttatcagaga tcaagtgtca atcggcaaca atgccgtgat catgatggga gcgattatca
     4081 atattggtgc agtaatcggt gaaaacacga tgattgatat gggtgctgtc ttaggtggac
     4141 gagcgacagt gggcaaaaac tgccatattg gtgcaggagc tgtcttagca ggtgtgatcg
     4201 aaccagcatc ggccaaaccg gttatcgtag aagacggcgt actggtaggt gcgaatgcag
     4261 tgattgttga aggtgtccat atcggaaaag atgcagtcgt ggcagcagga gcagtggttt
     4321 tagaagatgt ggcagcagaa actgttgtcg gtggaatacc tgcacgtgta ttaaaaataa
     4381 gtgatgatca gacaaaagaa aatacagcat tgattgctgc gttacgcgaa ttatagagga
     4441 gatggagaaa aatgcgcgaa atagatacct gtattgtggg ggcaaccgga ttagtcggcc
     4501 aaacgatgct caaagtgtta gaagaatatg attttccagt aggacgattg aaattattag
     4561 cttcaaaacg ttcagccggt aaagtttgtg tattcaagaa aaagaaacac actgtacagg
     4621 aattgacaga aacttcattt gaaggatatg atttagcgct attttctgca ggtgcttctg
     4681 tatccaaaga gtttgcgcca atcgcacgaa gtcagggact atttgtcata gataattcct
     4741 cagcatggcg ggaagattcg gatattccat taatcgttcc agaggtcaat ctggaagact
     4801 atacattgaa tcggctgatt gcaaatccaa attgttcaac cattcaagcg gtacttccgc
     4861 ttaaagcttt acaagaagcc tttggtatcc agcgagtcaa ttattcgacc tatcaagcag
     4921 tttctggttc cgggcaaaaa ggcattgaag atcttcatcg tacgcaaaat gacgaggaac
     4981 cagttttgta tccttataat attagccaaa cggtgattcc ggaaatcgat cagcctttgg
     5041 aaaatgggta tacaaaagaa gaacagaaaa tgataaatga gacccgtaag attctgcatc
     5101 agcctgcttt gccagtatca gcaacctgcg tacgtgtgcc aatcgaaaat ggacatggag
     5161 tctctgtggc agttcaactg caaaaagagt ttacgacgga agaaattaga gagtgtctca
     5221 gccagtttcc agggattcaa ttagaagatg atctagcagc acataaatat ccgacgagca
     5281 ttcttgctag aggaacagat atggtttatg ttgggaggat cagaaaagac acttctttag
     5341 agaacggatt gttgttctat acgactgccg ataacatacg caaaggagcc gctgcgaatg
     5401 ccgtacagat tgctgcagct ttaatgcaga aaatagcgga ggtggcaaga tgagtaatac
     5461 gctgaaagta gcgaaattcg gcggaacatc gatgtctcat gaagagcaat tcaaaaaagt
     5521 ccgtacaatt gttctttcag atcccaatcg ccaagtgatc gttgtttctg ctttaggcaa
     5581 acgtacgtca gatgatgaca aagtaacaga cttactttat ttgatttatg cttaccttca
     5641 gcatcatgtt gattggacac ccctttggaa aaggatttgt gatcgtttca ttcaagtaag
     5701 agatgttctt gccctgcagt tacctattga agatgaacta gcacgcatac agaaacaact
     5761 ggaagacgaa aagataactg aagattattt agtaagtaga ggtgaatatc tgactgctca
     5821 actgatggct gagtatcttg gttaccagtt tattgacgca aaagacctga ttttctttga
     5881 ctatgatggg aaaatcgatc aggaaaaaac agcacaggaa atcacaaaag catttaaaag
     5941 atatccgaaa atggttgttc cagggtttta cggggcgaac cctgcaggaa aagaaaaatt
     6001 attgggccgt gggggaagcg acattaccgg cgcgatactg gcgaagatgt tgcaagcgga
     6061 aagttatgag aattggacgg atgtttcagg aatcatgatg gctgatccgc gaatcatcga
     6121 tcatcctaaa atcatcgagg aaatctcttt tagagaactg agagagatgg cttatatggg
     6181 ggctaacgtt cttcatgaag cagctgtttt cccagttcaa gaggctgata tccccatcca
     6241 aataaaaaat accaatgatc ctttcgctca agggacaaaa atatcgaatc acgaaataga
     6301 aaaggaaaat ggactgacag gaatagctgg acgcaaggat tttctttcca tcactttatt
     6361 caaacgtcat atgtccgatg aaatcgggtt tatctggaaa gcaatgagca tttttgctaa
     6421 acatgagata tcaatcgaac atattccttc agggatcgac aatatcggag tgatcgtctc
     6481 agcagaagcg attgccgata agttatttct catcacgaaa gaattaaaag aaacacttgg
     6541 cgtggaagaa atcgtcgtga tcgaagactt agctttgatc tctgtcgttg ggggtccgca
     6601 taaagaattg attggattat ctgggaaagt gttatcgata ttgaacaaat tagaaattcg
     6661 cacatcgatc ttatcacaag gagcacagga gttgaatctg atcatcggtg taccgaataa
     6721 tcaatatgaa acagtagtga agggtatcta tgaagggatg gtgaatacgg atgctgaaag
     6781 aatatcgaat ggcactccat caaattcctg aaatcgggtt tcatgaattc aagacaaaaa
     6841 aatatttata tgaacaagta aaaaacagcg aggccgttat ccatgaaatc ggcgaaacag
     6901 gactactcct ttattttgat aatcagcaag aagaaacgat tgcttttcga acagatatcg
     6961 atgcactgcc agtcaaagaa gaaacgaatc tctcttttgc ttctaagcat gaaggttata
     7021 tgcatgcatg tggtcatgac ggacatatgg cgatgctttt agggttgact gactttataa
     7081 ctaaacacat tgaggatctt atttataatg tagtactgat ctatcagcca tcagaagaaa
     7141 tttccggtgg cgcacaaagc gttattcagt caggattgtt ggataaatat gaagtacaag
     7201 caatatttgg ctttcatcta tggcctggac tagctaaggg agaagttttc tctcgaccag
     7261 gacctttgat ggcacaaagc agtgaaacag acattatcat taaaggccag tctgcacata
     7321 ttgcttctag tgaaaaagga atcgatagtc tggaggcagc tgtacgattt ttgaagaatg
     7381 tttatgattt tgaccggcaa ttaccggcag agtggaatca tctaatgaaa tttggccaaa
     7441 tccaaggtgg aacgatccga aatgttttag ctggagaagt tattgtatcc ggaagtatcc
     7501 gttcttacag ttcagatatg caaaattatt tgaagagggg attagaccat ttagcagaac
     7561 agtttcactc tacagctact atttcttttc gctataatga cggctatcca gctgtaatca
     7621 accatagagg cttatatgca gcactgttag attcaaacaa actacatgaa ttagatgctc
     7681 cagttcttca agccgaagat tttggcatat atacgcaaca atatccatgt gtatttttct
     7741 ttttaggtgt cggtgatacc cctgctttac atgatccgac gttcgatttt gatatggatg
     7801 ttttagaaaa tggtgtagaa tggtacaaaa cgattctttt tacagaaaat cttcttgtaa
     7861 aaatcgatag cagttcaaaa gaggagcgag taaataatga tgaatttatc caatcgattt
     7921 agtcctaggc tttctagaat agaagtatca aaaatacgac aattcgatca gcaaatatcg
     7981 tcgataccag atgtgatcaa actcaccctg ggagaaccgg attttccaac tcctgaacat
     8041 gtcaagcaag caggaatagc agcgattgaa gaagactttt ctcactatac cggaatgaga
     8101 ggattagagg aactgcggga agcggcttgt atatttcagc aacagcgtta tggattaact
     8161 tatgatccgc aaacggaagt tttgacgaca gttggtgcaa cggaagcgat tgcctctgcg
     8221 ttgttatctg ttttggaaga aggagacaag gttcttattc cagcgcctgc ttattcagga
     8281 tatcagccat tagtggaact agctggtgct gaattgatcc cgatagatac ttctgacaca
     8341 gggtttgtct gtcagccgga acaatttgaa cgagcatttg aacaatatgg atcagctgtc
     8401 aaagcagtca ttttaaacta tcctaacaat cctactggga cgacattgtc tgcaaaacaa
     8461 ttgaaagcaa tcgctgaggt tttaaaaaaa tatccggtat ttgttataag tgatgaagta
     8521 tatgctgaat tgacttatag tggaacgcat atgtccatag ctacttatct tccagaacaa
     8581 acaatcgtga taagcgggtt atccaagtca catgcgatga ctggttggcg agtgggcttt
     8641 atttttgctc aaaaagccct tattgacgaa ctaattaaag tccatcaata tctggtgact
     8701 gccgctacaa cgatgtcgca aaaagctgcg gctgaagcgc taataaatgg ggtgaaagat
     8761 agcgagaata tgaaagagca atatcaaaca cgaagagact atctggtcca gcaattggcg
     8821 cctttaggat ttgaagtgac ccagccaaac ggtgcatttt acttgttttg caaactccca
     8881 gctgccattc aaactgactc ttggcagttt tgtgtagaat tagcggaaca ggcaaaagtt
     8941 gcgtgtatcc caggaattgc atttggccca gaaggagaag gttatatccg gatttcttat
     9001 gctagcagca tggaaaaatt acatgaggcc tgcaaacgga tcaaagactt tttaacagaa
     9061 cagtgattca tagattttat caaaaaaaca atcgttaatt ttgcggtata ttctgcaagg
     9121 agacgattgt tttttaaatt tagttcaaat gaacgcgttt cttttgattc ataactaatg
     9181 taatgttaag tttaaattgt ttagaatgat tttaaaagaa tattcaagga ggtttcatca
     9241 atggaaaaaa tcaaagttgt cattgtaggt gcatctcatg gagggcatca atcgatcttg
     9301 gaactattga cacgatacgg tgaaaatgta gatatcacgc tatttgaagc aggagactat
     9361 atttccttca tgtcatgtgg tatggagctg tatttagaag acaaggtaac agatgtaaat
     9421 gatgtacgta atttcagacc agaaaacttt ccgcaatcca atgttcatat tttaaataat
     9481 cacgaagtaa aaacgatcga tgccgataaa aaaacagtaa cggttactag cgcatcagat
     9541 aacgaaacaa aagaatatcc ttatgacaaa ttgattttga gttctggtgt caaaccaaac
     9601 aatctgccag taccaggagc agatctagaa aatgtctact tgatgcgtgg atacaattgg
     9661 gcaaccaaaa tcaaagaaaa attaaatgat ccggctatta aaaaaatcgc tgtcgtaggt
     9721 gcgggttaca ttgggatcga agcagcagaa gcaagccgaa aagctgggaa agaagtcgta
     9781 ttgcttgatg tgatcgatag acctttaggc acttatttag atgctgagat gacagatatt
     9841 ttagaacaac acctaaatga aaacggtatt gaagttttga caaatgcaaa aatcgaagct
     9901 tttactggta acggaaaagt tgaggcaatc aaaacaagtg aaaaagaaat cccagcagat
     9961 ctagtcattc aagctgccgg tgtgaaggca aatacagaat ggttaaaagg gatcgtcgac
    10021 ttagatgaac gtggctggat ccaaacaaat gaatacttgc aaacgaatct tccagatgtg
    10081 tatgcagtag gagatgctac acttgcttat tctatcccag cacgcaaaaa aatgccgatc
    10141 gctttagcga ctgttgcacg tagagaagca cgttatgtag taaaacatct ttttgaagaa
    10201 gtaccaaaca aaccgttcgg cggagtcgta ggttcctctg cattaagtgt ctttgactat
    10261 cattttgcag caagcggatt gaatagcttt acagcagcaa aagcaggagt agatgtacga
    10321 gtgtcttatt atgaagatac gttacgacca aaatatgtac cagaagaaca aggaaatcca
    10381 aaagtgtcca tccaattatt ctcggatcca atgacccatc aattgctagg aggagcagta
    10441 ttatctacct atgatgttac aggtcaagga aatgtattgg cactagcgat ccaaaacaaa
    10501 ctgactttag aagaactagc agaagctgac ttcttcttcc agccaggctt tgaccgacaa
    10561 tggagcatgt tgaatctagc cgctcaacaa gcgttaggag aagaaccatt tgtagaataa
    10621 gaaaacgaaa tatttgaaaa gaggttgtga aaaagctgcc ttgcatcaag taataagaac
    10681 agccaagtaa aaacagcttc ttatgttttt aacttggctg gacttattac cgcagatgca
    10741 agcttttgaa caccgtttat tttcaaaaaa gggactgtga cacgatttgt gtaccgaccc
    10801 ccaaaagtta gaccaaaaaa tctaactttt ggaggtcggt attttttatg gttaaatata
    10861 cttttgaact taaattaaag atcgttcatg attatttgga tggcaaaggc gattggaccg
    10921 taaaaatcca gatcaagaga ttgaagcgga aatgcttaaa attcgggaga agcacaagga
    10981 ttatggctgt ttacggatga ccaatgaact aagaaaccgc ggtttctcta tcaataaaaa
    11041 gaaagttcag cgcttgatca agaagctagg aataaaagta acggcataca cgcgtaaatc
    11101 acgtcgctac aattcttatc gaggaaaagt
//
LOCUS       DACCYH010000098        11100 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.102, whole genome shotgun sequence.
ACCESSION   DACCYH010000098 DACCYH010000000
VERSION     DACCYH010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.102"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            390..1829
                     /locus_tag="IVY85_002455"
     CDS             390..1829
                     /locus_tag="IVY85_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8045427.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2124..2342
                     /locus_tag="IVY85_002456"
                     /pseudo
     CDS             2124..2342
                     /locus_tag="IVY85_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2591..2806
                     /locus_tag="IVY85_002457"
     CDS             2591..2806
                     /locus_tag="IVY85_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045428.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(3023..3279)
                     /locus_tag="IVY85_002458"
                     /pseudo
     CDS             complement(3023..3279)
                     /locus_tag="IVY85_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(3466..4377)
                     /locus_tag="IVY85_002459"
     CDS             complement(3466..4377)
                     /locus_tag="IVY85_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8045429.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(4396..5400)
                     /locus_tag="IVY85_002460"
     CDS             complement(4396..5400)
                     /locus_tag="IVY85_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP8045430.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(5397..7520)
                     /locus_tag="IVY85_002461"
     CDS             complement(5397..7520)
                     /locus_tag="IVY85_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8045431.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(7525..9972)
                     /locus_tag="IVY85_002462"
     CDS             complement(7525..9972)
                     /locus_tag="IVY85_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8045432.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(9959..10348)
                     /locus_tag="IVY85_002463"
     CDS             complement(9959..10348)
                     /locus_tag="IVY85_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8045433.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(10408..10911)
                     /locus_tag="IVY85_002464"
     CDS             complement(10408..10911)
                     /locus_tag="IVY85_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8045434.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(10924..>11100)
                     /locus_tag="IVY85_002465"
     CDS             complement(10924..>11100)
                     /locus_tag="IVY85_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045435.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLSLFNRIVGA"
BASE COUNT         3238 a         2330 c         1998 g         3534 t
ORIGIN      
        1 tgatatttag tataatccta tggaattgaa tatcatagca atgtatttga aaagcaaatt
       61 ggaaaacaaa taggtgcaac accataaata tgaaaaaatt gggaaaaagt agaatggaaa
      121 tttgcttacg tctcacgaga tagctcacac tattaatctg aagaacaatg aagtcgactt
      181 ttagaaattg ctagaagaaa gtaaagcgaa ttatcactag ttactataga gtaagttcaa
      241 ataaagcatc tccaacttac atggttacat gaggatatca aatcgaaatt actcaagctt
      301 cctatggcta gagtcgcgag aagttgttgg gcagtagaca taggttatat tgttcttagc
      361 tacacgatca ttagaaagga aatcctatta tgtatagtta tcaagaagat gcaaaagtcc
      421 aaaaaaatcg ctggtggatt ttgattgcta tatctatgtt taccttcatg tccacattgg
      481 atggcagtat tgtcaatatt gcactaccta ccatctcaga tgatatgggc ataccaatga
      541 atcaagcgga atgggtagtg tccgtttatc tgatgattgt ctgttcttgt ttgttgctgt
      601 ttggtaaaat tggcgatagc cgtgggaaaa tcaaagtatt tcggattggt acgctgatct
      661 ttgtcttggg atcgctgttg tgtgggttca atcattcgct aggtatttta ttgttcgcac
      721 gggtggtaca agccattggt gctagtatga ctatggctac aaatgccgga atcatcacgg
      781 aagtctttcc catacatgaa cgtggtcggg cattgggagc aataggtgcg tttgtttcat
      841 taggctcgat tgctggacca ggtcttggcg gcttgatttt gtcccaattt tcttgggcgt
      901 acattttttg gatcaatgta ccggtgggaa tcgtaacgat gttgattgct gaaaaattct
      961 taccaaaaga tatcaccatg agcggccata caatcgatgg agggggcttt gctgccattg
     1021 cggctacgat tatcagcttt tttggagcaa tctttttggg gcaagaaagt ggctttttcc
     1081 atccagtccc ccttgtactg ttcgtaattt cagtgattgc ctttgtcagc tttttaaaag
     1141 ttgaaaagaa agcagcctct ccattgattt ctttcggtat ttttaagaac aagattttta
     1201 ccatgagctt gattacagcc ttgttgattt tttcttccaa cttctttgtc aatgtagtga
     1261 ttccttttta tctgcaaaat gctcgcgggt tgagtcctag ctatgctggg ctgttgatga
     1321 tggtctttcc tttgatgatg gtcgtcgggg cgcctttaag cggctacctg accgaccggg
     1381 ttggcccgga gattttggta ttagtaggct tgggcttatt gtcggtaacc caggtcatgt
     1441 atatgttttt gtcagtggac tctcctttgt ggctatacat tgtcgctact gccatcatgg
     1501 gtgccggcaa tgccttgttc cagtccccca ataatacgat ggttatgagc tcggtcacta
     1561 tcgaaaactt gggactggca ggctcgatga attcctttgc ccgaaatttg gggatggtca
     1621 gcgggatttc gctttctacg acggtcttat atcaagcgat gtctgctaag atgggccagc
     1681 aagtaactaa ctatatcgca aatcggccgg atgtcttttt atatggaatg aagattacct
     1741 ttttaggcag ctttgttttg tgttttgttg cctttttcct gactatctgg cgtatcaaaa
     1801 aacaaggagg tattcagaaa aatgtctgaa aaaaccactg ttgaagaatt tttggaggcc
     1861 tgcgtatggg tgagtgggat ttccatggat gatggaagat gaagtgaact tctaaaagcc
     1921 gaaaaactca agtattatct tcaaaatact agtgctcaat gagatgcttg atttgatcac
     1981 aagaagactg cgaaagccgc tggcaaccgc aagctaggta tcccaccgat ggactacgtt
     2041 ttagaggcta ctggttatga atacggagcc aacgctctca tctgcataag aatacatcac
     2101 ccagattatg taaagatttt cgatacttct gtcaaaaaat cattaagata gataaatgat
     2161 ttccgtctag tcaaattggt tcagaatgta gacacacaga tggtaagaag tcactcacaa
     2221 ttcgagaatg gacttgtcct atttgccgta tacatcataa tcgaaacgtc aacgatagta
     2281 tcaatatttt gaccaagggc tatgaataca agcattgcct tagaaaacat caagaacctt
     2341 aggaacaacg gggatagctt ggttaataag agataccgct gtttaggctt gcaagtatac
     2401 tcttttatca agaattccgt ggctttagtc atgagaggct taatactatg aaaattttct
     2461 ttagcatttt tgatttaggt tccttttcta attataataa gatgaccact acagtaacct
     2521 actgaaatat ataaacagcc actgtagtac cattttcaaa gtagtctatg agaggagaat
     2581 cttcattctt atgaacgctg gaattgcgga tatgaattta ataaaaaaaa cccttaatga
     2641 ttttacttcc aattcaatat caaaaggtac tggtatcaat ttaagcacaa taaaaaagct
     2701 gaaaagtggt gaaagatcag tggaaaagct gaatctttta gatgctataa aaataacgga
     2761 atttgctatg aaaaatggaa aagctgagat tgaaatatgg agataattat cgtctagtga
     2821 attacttgcc acttagctga atttaacgat ccttggtgct tgaaggggga gattcttggg
     2881 aatagagcat acttgcaagc ctatgtcttt actaacaacg atatctctta ttaaccaagc
     2941 tatccccgta gttctaaggc ttttactttt atctaagcaa taatcgtatt caaaggaaat
     3001 ttttgaccct tagaaagtct cattttatca gacgtgagct taggcattgc tgaattttgt
     3061 taattcggtt ttaggttgtg ctttgagcat aatatgaaag tattctttat cacgatttca
     3121 ttcatctaag gtaatgtgat gtgattctga aattctttca aaggcagttt ttgcataagc
     3181 tgacattaca tcatcaatga catgtttaca atgtttcact actaaaataa gggggtaacc
     3241 aagaagaaaa actgaatgat tatttgtatc taattccatg gtatatcaac catttcctat
     3301 atagactgat tatgacatgg attaaaataa aaaggcacta tgtcttaatt gtcggttttc
     3361 gagttaccga atggcattca tcacccacct atagaggatg ggcgacttct gcctgaaata
     3421 agttacacta cttataactt ctaataaaat tagattgagt cattattatt taacatcaac
     3481 gattttccag tttccatttt tttgtaatac taatttgtac tgaaagtagc tagtggtttt
     3541 tgatacatta tcaaggtatt ttatctttgt actcactaga aaattttttc cgttttgtag
     3601 ataaactgga ttctctaact ccgaaaactt taagtcaata ttgattggtc gcatcgcacc
     3661 attttcaaca taatactcca attctttttc agaagctgat ggataaagtt taaaaaatgt
     3721 ttctaaaaaa tcggtgattt cttccgttgt tttggcatca atttctgaat cgttttctac
     3781 ttgttttgct tcatattttg ccttgtctgg ctttccaatc attgttggca aactagttac
     3841 taaatgattc ctgttttcat cttcaaagat tgtcactcga tatgcagaag aaattgattg
     3901 cgtctttttt ccctctgtaa tatgttgctc cactaaaaat gaaacgacaa attcttccga
     3961 gtgtttttca acatctagaa tttttacaga ttgaacttcg gaactcgtgg gaatatctgc
     4021 tcgaaccata tcttggatta aagcgagacc gttttctgta aggtactgtt cgagattact
     4081 cattctcttt tcaattgctt ctttgttatt tttccatgaa aaatattctt tcgcaaaatc
     4141 ctctgtaaaa ctttcaacgc cagaagtatt cactaccttc tcttttataa ccactttttc
     4201 atgagttgta tgttgatcaa tggcggtgaa atttttataa acgccaaaag caagactaca
     4261 agacaataaa agccaaaggg caagcaccat ttttcgatgc ttacccacgg atacaactcg
     4321 ttcttttttc ttttttgggc tcttttcctt ttgattccgt tcaattttta tcttcattct
     4381 ttttcctctt ttctcttatt tgtatcggcc agctccgata agatgctgcc gccaatagtt
     4441 ttccgttaga ttcgtccacc ccactggatc tcctgcatga aacatgcgat tctcccctgc
     4501 gtaaatgccc acatgggtca cataagtacc ggcatcatag gttgaatgaa agaaaactaa
     4561 atcacctggt ttggcatctt ttagatcaat atgagtcatg gcatcatatt gatcctgagc
     4621 cacccgagga agtttcaggt caactttggc ataacaccat tgggtcaagc cactacaatc
     4681 aaaccccgtt tctggacttg agccaccaaa cacataggcc gttccttcat acttaaatgc
     4741 ctcatcaaaa atttcttgga ctattttgtc atcaaactga gttgtagtta ggtattgctt
     4801 cactaataaa cagtaaaaca tattgccata attataccgc cacccaccat tttccttaat
     4861 cgcgattggg ttggggtaat cgactttttg accacctgat ttttcctttg aaaaagattc
     4921 agctaaagca aaactatgct tcttcccgtt tttcccgaca tgatctagat acccgccacc
     4981 atagttatag gcctgaataa tactatcttg atcacaccct agttctttgg aacgcttaac
     5041 caaagacgca aaatagagac agccttgttt gatggatgcc tcggtactta atgaattagg
     5101 tggtaatccg gctgactcag aactttgcat aacgtcttcc attgtgccac cagattccac
     5161 ttgaataatc gccaaaagaa ttggaatata ctctgttata tccgattctt tggcatactt
     5221 ttccaccatt gcacggtgtt tcaatacttc ttctgagaca ctagttccgc caggagaagt
     5281 gccagaacta ccatttccct cactgtcttc accaaacaac aaacccacac acatcaaaat
     5341 acccataaaa gagatggtca gaaaagttaa tgaaataaca cccactttct tcaagttcat
     5401 tttttctcac ctgaccttga ttttggacga tggttcattc ttttttgtac tttttgattg
     5461 gtctgctttg gcagagtgga cctctgtttc acttgttgga aagaattttt cttacttgct
     5521 gattggcttt tcccttttga aatgatttct tgactagtta gttttcgttt gatttcggga
     5581 ttcagatttg ctctcacaat ccgctttggt gcttcttttt tcagcatcat atctttctga
     5641 ggttgagctg ctttatctat tggaatattc gcccttcttt ttccctgacg atttgttgct
     5701 tcactcagta tcttgcgttt ttcgtccatt ttcgcatttc ttcgttgtcg ttgtttttct
     5761 cgcaaatcca ctcgatccgc tttttcttgg aacagtccgc gcttgaattc ttctggtgct
     5821 tttttcgact tttcgattcc tttatggaga ttgtatttca aattcgttgg cgtattcctt
     5881 acctgatcct ttacttgttt agtcttatct ttcaccaaag cttgggtatc caaaactttg
     5941 ccgatttttc tcccagccaa attcattcgg gattgtttct tattatttga aactggattc
     6001 tccttagtta agtcagatag tatttcgtga tcgttcggta atcgtgaatt ctttcgtaga
     6061 gaagaaccgg atggcttaaa tttggatttt gattgttcct ttgccaactt atttccgact
     6121 gctgcaccac caccaaaagc caaagctcgt cccacatttc gattgagttg ccgcaatttt
     6181 cgattcatta acatttgcgg ttttcgcatg acgcgtcttc ccattgactg gctatcatta
     6241 ctctggaggt tgaacatact cataatgtcg cccaatttga aatagatacc ggcaaaggtc
     6301 acaatttgca gaaaagcaac catgaaaaat ggataggttg ccgaaatatt gaaaaacatc
     6361 gtggagatgc taaatgcagt cgtaatcacc aaagtaacac ccgctcgcat cataatcgta
     6421 ttgaataaac gaataatcgc cttctttcct aaactttcat aagtcggcaa catggacaat
     6481 aagaaactaa ttggcagaaa catcgcaaag ataatgaaga gaatttgcga gaacaacata
     6541 atccctgtta gaagaaaaac aaagaaggaa atgccaatat tgaacaatac caaaaagacc
     6601 acggtcccta aacggttcat ggtcttggta atactgagat tcgcattgtc attgtcttca
     6661 atctctgcct tgaccgcttc ttcccgatct ttgcctttgt tttcatctgg actcacagat
     6721 agaatcttgt tgacccggtc ttcaccaatt tcttctatgt tggaatcatc aaattgcagt
     6781 agtaaccaag gttgttggac ttgaatcgaa aacaaactat cccgaatcaa atccacacta
     6841 tcttttcctt ggctttctga attgggtaca acgatctcgg ttccaagggt aagggccgct
     6901 tcactaacat ctgagctaaa gtcattgatt tttgtgatat aggtaggcgc ataagcaatg
     6961 aacgaacccg atagtagaaa aatcatcaca aaattaatca cggctcgaac tgctttcgtg
     7021 gtttctcgtt ttaataatcc ggtatacgcc acatacaccc ccagagctaa aatcatcaat
     7081 aatagaaacc caaaataaaa tccggaggtc tggagtccat tttcagtaat accagccaat
     7141 gtttggatat tctttccaat actttcagcc gtatccgaaa tgaaatctaa tttgtaagcc
     7201 tcttgaacca cataacccgt ggcgttcgac aaatacaaac tcaccgtcca aatgaaatta
     7261 gtaatggcat aaagaccata catcacgctt ttccctaagc catcacccca attccaaggc
     7321 aaccaatccc aagagctatc cacaaagaaa tctagttgat agttgttcag tgaatatttg
     7381 gaatatagat ttcctgcttc aactgtatca tcaactagcc ctgtcgcctc ggctaccgta
     7441 ccaactaaac tcaatagaac tatcagagtg agtatgctaa taaaaacgat tcccacaatc
     7501 cgaaaaagtt tctttttcat cccctcactc cattctctct tgaacaggtg gacgggtatc
     7561 aaaggcatgg aagagttctt caaagactgg atgtacctga accacgccta cccgaccata
     7621 caaatcctgc atcaagcatt ggccattttc caaatctcgt aggcgttttt gattgctttc
     7681 atcttctcga tccaagccaa agaattccaa ggttttcttg atttcatgaa tatcggtact
     7741 gcgaaacgca aacttcaacc cgatattatt cttcaagcgt tcatccagta aatcatcact
     7801 attttgcgtg acaaagtaaa ccccggcatt catagcccgc ccagcacgaa cgagcttatt
     7861 cgataatgtt ttcccttgcg ctacttgaag aaaactccac gcctcatcta agtctacaat
     7921 tttgaagatt tctcgattac tatggataaa gtccaaggca aaagtggaaa tgacgattag
     7981 catggaaaca ctaagcaatt ccatggtggt atattcttca aaagtggttt ctgcatctgg
     8041 taaaaccaaa tccgctactt gaatgatatt taactgccta tctaaactaa tcgactgggt
     8101 aatcgaacca tctgagaaaa gcaaatgggc aaaatcataa tcggtaaagg actcgatgtg
     8161 atccgcgata ttttccgcaa ctggtgagcc ttcttttcga agctcattaa ttacttgtaa
     8221 taatccccgt tgattgcttt gggtaaccgt ccgaatggcc ttacgcaaca ctggaaactt
     8281 ctccccatct cgactagaaa ttcctgttaa aaaggtcaga atatcaattg ctagactttc
     8341 agaatccttc tttttctcca taatcacata aggatcaagt aatccttgat tttttggctt
     8401 actagttaag ttgagaatat tgatttcatc cgcaatttct ggcaaggttt ctttccagcc
     8461 accccgttcc ccttttggat cgacaatcac agcttgtcca ccaaaaagga cggcgtaata
     8521 aactaagaga ttgttactaa aggactttcc gccaccgagt gaaccgagga atgccgctgc
     8581 tagcgcattg gtaactgaat ccttgacccc ttgtgaggcc aatgccggtt tcaggtaaac
     8641 attgcgcccc gtatctacgt tataaccaaa gtaaatgcct tccaactccc ctaacatctg
     8701 agtggcgcca aaaccaagtc cggcgagaaa atcagacgta acatactgga tataatcatt
     8761 catgtatcgc ttactagaag gtaagaattc ttcatgtagc cccatcatat cgccaaaagg
     8821 ccggactagt tttacattgg tatcatcata aaaatctagc acttcatcac accggcgctt
     8881 tagttcatct actgattcgg cacttactcg aaccacatag ctcagtttgt acatggattc
     8941 tttggactga tccaaggtag tttccaattc atctacggaa tccaatgcat ctaagacatt
     9001 ggaatttgtt tcattgtcag attgataggc atggttgtcc aagtctttta attccttttt
     9061 cttattgcga acggtagcaa gtgccttttt gttcgtcaca atctccacat tcatactagt
     9121 gtcaattggg aatgtaaatt gttgttgctg gtaatagaaa agttctgagg agggaaactc
     9181 catttccccc acaattgtat taattgttaa gtacgctaca tatgattcat gattctcatg
     9241 ttccatgcat aagtaccggg gcttttcttc aatcaaacaa cgactgggac gtaataagtc
     9301 gtaacgtttc accaaagttt ccgacttcag ctttttcttt ggtagctgaa attcatattc
     9361 ttcaaatggt gtaccctttt cgccatagat atgttcaatc aaatacatca gatcactcgg
     9421 tgtgactcgt ctgactttaa atcttcgagc caatttactt tccattagtt tttcaagctt
     9481 ggtatatcgg cgaatttctt cattggataa ggaaacaaaa tcgcccatca aatgatgatt
     9541 cactccatag acaaattctt gaaagccgga gaagaaagat ttcttgagat tctttagatt
     9601 gacctcttca tctgtcgcaa tcagtttgaa gccaataaaa aagcgataat caatctgact
     9661 atcgccaatc atggatacta atgcctctgt ctgtaaatca attcgttgtt tcgctacttc
     9721 tgttaatcgc ccggtaattt ccttttttga tcgttcttgc gtggcacgaa tactactttc
     9781 tgtggcaatt tgtaaggcat gaatcttgcc ttcacgattt tgcgcaatca attgacgaaa
     9841 gttatcgtgg acttgatatt tctgttcagg tgacaaaaag gagtaattgt aaggtattaa
     9901 ctcatagtag gcaaaacatt ctccctcttg attaaagaca agattatttt caatatactt
     9961 aatcgggtac atagttcaca ctccttacca gagtaatggg ttcgtcccat cgtttcttct
    10021 gttctttgac tttctttcct gcgaaagtga ccttaaatcg taagtgatag ctaataaaag
    10081 atcgtaaaaa tcccaatggt ttcttaccgt caaacgtttt ttgcgacatg aaccaagtaa
    10141 ctgccaccgg tatacctaag tattttaaaa aggccccctc tatcatagat agtggaggca
    10201 catgggcaaa tacgatgaca aacagcaagg ataaaacaaa ccaagccatc tggttaaaag
    10261 tcacaggaaa aggcagttga aagtcattaa ttgcataaat gaccttttca actgcccaaa
    10321 tactggtgta gctttttatt ttcttcatca aatcattcct ttaaaaagaa ggatagttta
    10381 gaaatcaact acccttcaat aattttgtta atatggatac tcaaaaacac ctcgactcgt
    10441 taccaaataa ttgcctccaa tctccaagtc tctaccacac gcatcaaaat caatatacgt
    10501 cttcaagtgt tctggtactt ctccaaaatc accagcttct acaatcaaga aatatgctac
    10561 atcactcata tcatcgcaat ctggataata gataatatca tccacatgct ctaccatctc
    10621 ctcaaaagaa ttgaagaaag catgttgaat ctcagaagca acttcaccaa ctggcgttcc
    10681 ttcaagttcc tccgctaagc cacacaaccg attaatttcc tcaattggcg tatactcatc
    10741 aatctcaaag ggaagttcat aatcatgaat tgcatattcc tcgtattcgt catttaaccc
    10801 aattcgttct ttcacttcat caaaatcgac aggtggggta aaccacgccc ccaccaattc
    10861 accttcgtta tatttcccga ggttcgcaat ataaacacgc atctgttcca tcgtgcttcc
    10921 cccttacgca ccgacaattc ggttaaacaa actgagtaag acatccttca ccccagaagc
    10981 attaaagacc aatccgaccg caatcaaagc aatcaccaaa aagccaatca gtttggaaaa
    11041 ctcccgctta aatcctaaaa acaaaccaat gaccacaatt gccagtaaga ctaaggattg
//
LOCUS       DACCYH010000099         9888 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.104, whole genome shotgun sequence.
ACCESSION   DACCYH010000099 DACCYH010000000
VERSION     DACCYH010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.104"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(109..2229)
                     /locus_tag="IVY85_002466"
     CDS             complement(109..2229)
                     /locus_tag="IVY85_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8045436.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2459..3637
                     /locus_tag="IVY85_002467"
     CDS             2459..3637
                     /locus_tag="IVY85_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP8045437.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3653..4411
                     /gene="aroD"
                     /locus_tag="IVY85_002468"
     CDS             3653..4411
                     /gene="aroD"
                     /locus_tag="IVY85_002468"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP8045438.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4434..4919
                     /gene="ybaK"
                     /locus_tag="IVY85_002469"
     CDS             4434..4919
                     /gene="ybaK"
                     /locus_tag="IVY85_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP8045439.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4990..6243)
                     /locus_tag="IVY85_002470"
     CDS             complement(4990..6243)
                     /locus_tag="IVY85_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8045440.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6360..7709
                     /gene="gdhA"
                     /locus_tag="IVY85_002471"
     CDS             6360..7709
                     /gene="gdhA"
                     /locus_tag="IVY85_002471"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP8045441.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFISGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7821..9167
                     /locus_tag="IVY85_002472"
     CDS             7821..9167
                     /locus_tag="IVY85_002472"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP8045442.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9475..9861)
                     /locus_tag="IVY85_002473"
     CDS             complement(9475..9861)
                     /locus_tag="IVY85_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8045443.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3150 a         1800 c         2024 g         2914 t
ORIGIN      
        1 ctgagccaga atgaggttca ttctgaccca gatttgattt tgtacaaccc atcagtcctt
       61 attgacaaag agcctaattt tgtcgctgct ccttttaaat ttattttatc attcggaaga
      121 acttgtggtc gttccgtgaa gttcttggta cgtttgcgtt ttataaagat caacagttga
      181 ttgtccgttg tgttcactaa agacactagt tgacttgtca ccaagagatt tttcagcttc
      241 ttttaatttc tctaagccat tcttgtagtc ataatcactg gtatcaattg ggtctaatcc
      301 actattcgta tagaaccgaa gaagatcacc attattgatt tggtcagaaa cagctaactg
      361 tttatcaact ttttctttca gtgaatcaat ttcattctgt acttcctcag taggttcagt
      421 aatagcttca ccagtttcgt tactatagat cgtaccaccg tagtaagtat attttggtgt
      481 aacaaaatca ccatcacgga aagaaacaag ctgttcatga tctttcgaga atagatcttg
      541 tcctaactga atataatttt ttgtatctac acctagtaag tgaagtaatg taggcaaggc
      601 atcaacttgt ccgccgtaag tgtgattgat tccgccctgt gtttgtccag ggatatggat
      661 catataaggg acacgctgca tttgtgcatt gtcgtagttt gtccaagtag aacttgtttt
      721 tccaactaat tcagctagat ttttatttct tgaattcgag acaccgtaat ggtcaccgta
      781 aaggacgatg actgaattat catacaagcc gctttctttc aaataattga agaattcttg
      841 tacggcggta tctaaataat tggctgtcgc aaagtaaccg ttgatcgttt catcatttgt
      901 attggctaac gggaatcctg cttcgtcatt ggtgaatttt gaataaggat aatggttaga
      961 tacagcaatg aattttgaat aaaatggttg ttgcagacgt tccaagtatt gtacggattg
     1021 ttggaaaaat ggtttatcat gcaacccgta ttggaacgaa ttgtcatcat taacatcata
     1081 ataagaagaa tcaaagaaat aatcatatcc taaattcttg tatgtctcgt ttcgattcca
     1141 gaatgtacca gcgttcccat ggaataccgc actagtgtaa ccttgtgttt gacttaaaat
     1201 atcaggtgca gcttggaaag tattttttcc gcctaattgt gtgaacaagg agccttgatt
     1261 caagccaaac aatgaatttt ccatcagcgt ttccgcatcg cttgtttttc ctgcttttac
     1321 ttgatggaaa aagttgtcaa aactgaacgt gctgttgctg tggtaaagac tattgatgaa
     1381 aggtgtaact tcatgctgga gaccattctc atctgtcaat ttataatcga tcaggaattg
     1441 ctggaagctt tctaaatgga tataaatcac attttttcct ctagcgagcc cgaacaaatc
     1501 gtcatttggt gctgcataat gttcttttac ataattttct acttctttca tatcattggg
     1561 acttgcttct gcccgcactt ggtttgtctg gtaagtttga acaccgtcat aaaccatgaa
     1621 agcattcaag ccaagatatt tgaccaaata atcacgagag aatgttcgag tcagcaattc
     1681 tggacgatca gcttccgcca taaaaagatt tcctgagaaa atcataactg ctaatgtaga
     1741 gatcgcaaaa gccattcttg ctcggatcgg gcgagggtcc aacttgattt tttttgcaat
     1801 cagcagaaca gtcaagataa taaaatcgat ccaatagatc acatcatatg ggcgaaacag
     1861 tcgaatggca ctttctccca gaccactcgc tactttccca gcacctaaaa tcgtgttgat
     1921 cgtaatgaag tccgtgaatt ctcgataata agtgacattc gaaaaaacaa gcaatgtcaa
     1981 aagaaaataa atgaccatca tcgtgatata agcaactcta gggcgtctga tataaagagc
     2041 aattccaagt aataacaatg tagttgcaat agggttgata aacaagataa agtattgaat
     2101 gccactttcg attccgagat ggaaatcaac taaataggca aaaatcgttt tcaaccaaag
     2161 taaaacgact agtaaagaaa aaaatccgag cctcttattc aaaaaggcag gtgtttttat
     2221 acatttcaag ataatcttcc tttctgcact tcttaatcac agaagtgtcc tgaaaccatg
     2281 ttacacagct cacttattct catttatttt actagtatga caatatagtg tcaattgaaa
     2341 ccagaagttt atgctgtttc attaaggttt tctttaagct tcatgaaaaa atgagcagta
     2401 agacaagtcc tatgtttttt gttatacttg ggaagagaaa gctaaagcag gtgaaggaat
     2461 gaaagtaaaa gtaaaaaaaa gagcaagcaa tcgattgaaa aaaggatatc cactgatcca
     2521 aaaagaagac cttgctcatc cccaaaaaga aaccacgcaa tgggtggaat tagttgattc
     2581 gcaaaaccaa tttatcgcaa aaggatatct aggaaagcag aataagggaa tcggttgggt
     2641 attgagccag cagaacatcc catttgatca atcttttttt gaagaatttt ttgtgaagtc
     2701 aaaagaaaaa agaactttct ttttttcaga tgattctact accgcttttc gtatattcaa
     2761 tggagaagga gacgggatcg gcggtctgat tgttgattat tatgcaggtt atgctgtttt
     2821 ttcttggtat aacgaaacgt tatatgcaca tcgtgaatta ttgattcaag cttttcgtga
     2881 aatctacccg gaaatcatag gaggctatga gaaaattcga tttgaatcag taaatctgcc
     2941 tgagagccag ttcatttacg gaaaagaggc accggaaccc ttgatcgtaa aagaaaacgg
     3001 agtaaaattt gccgtttatt taaatgaagg attaatgact gggatctttt tagaccaaaa
     3061 agaagtacgg ggatcattag cagaaggttt cgcttctggg aaaacagttt tgaacatgtt
     3121 tagttatacc ggcgcctttt ctgttgctgc cgcaatggga ggggcaatcg aaacaacaag
     3181 tgtcgatcta gcaaaacgca gtttaaaaaa aacacaggaa cagtttgaag tgaatggttt
     3241 ggatgtcgat cagcaaaaaa tcattgttat ggacgtattc gagtatttca aatacgccaa
     3301 aagaaaacaa tttttatatg acatgattgt attggatcca ccaagttttg ccagaaataa
     3361 gaaaaaagtc tttcgcgtag caaaagatta tggggaactg gtcaaggata gtctggatat
     3421 tttagcagaa gacgggatac ttattgcgtc tacaaatgct gcgaatgtct cgatagaaca
     3481 attccaaaca atgattgaag aggaattcca tgccaaaaat gtttcttttg aacaacttgc
     3541 tttatatcgt ctgccaaaag atttccagac gacggatacc tttttagaag gcaattatct
     3601 gaaagtgttt gtttatcaaa ttaaaaataa ccattagaag gagcgatgca gaatggttcg
     3661 ggtaaaggga ttgaatatag gaagcggtag accaaagatt tgtgcgcctc ttatcggggc
     3721 cgtccgagag gaagtattac gtgaagcagt tcttgcacgt gaagcagggg ccgatttagt
     3781 cgaatggcga gtcgatcatt atcgtgaagt tttttctttt gatgaagtag cagaaacatt
     3841 ggttcttatc catgagatgc ttgatggact gccgttatta tttacgtttc gtaccttagc
     3901 tgaaggcgga gaacaagaca tatcgatccg tcagtatcgc gacctttatg aacatgtgat
     3961 acacacagga ttagtagata tggtcgatat cgagttgttc aaagtagaaa gtctaggaaa
     4021 aagactgata gaggaaatca agtctttaaa cattccttta attatcagta gtcatgattt
     4081 taaagaaaca ccagcagacc ctgtcttgct ttaccgattg aatgttatgg aacattttgg
     4141 agccgatatc ggaaaactgg cagtaactcc caataatgaa cgagatgttt tgcggttgat
     4201 ggagttgact agacgggcaa atgcttttgt ctccatgcct attattacga tgtccatggg
     4261 aaatcttggt aaaatttctc gtctggttgg cggaacgacg ggttccgtta tgacatttgg
     4321 ttccttaagc agcgattcta cttctgctcc aggacaatta ccggtcaaag cattgaagca
     4381 aattattcaa ttattaaatg aaccgttgta gaacaagttt gaggtgatat tttttggcaa
     4441 agaaaaaaat agtaaaaacg aatgcaatcc gtatggtcga acaaaaaaag attccctata
     4501 cagagcacga atatgagtgg gacgaaagcc acctaagtgc gtcatcggtc gctgaacagc
     4561 ttccagaaag ccaatctcgt atatttaaaa cactggttgc tgtgggcaat gtgacggggc
     4621 ctcttgttgc tgtgataccg ggagaagccg aattgaatct aaaaaaatta gcaaaagttt
     4681 cagggaataa aaaagtagaa atgctccatt taaaagattt ggaagcaacg accggataca
     4741 tacgtggtgg ctgctcgcct ataggtatga aaaaactatt tcctacttat ttagatcaaa
     4801 ttgcagaaag ctatgaacaa atcattgtct cggctggcag aagagggcta cagatggagt
     4861 tagcacctca agatatctgt gcattaacga gcggacaatt tgcagatata aaacaataaa
     4921 tcatacgtat aaaagaaaga aggaggccgg gatgttgttt gtccagtctc cttctttctt
     4981 ttttatacat tatgctcttt gcccggcatt cattcctgct acatgccctg tagcgaaagc
     5041 cgctgtgata ttaaagccgc cagtataacc attgacatcc agtaattctc ccgcaaaata
     5101 cagaccatca atgattttac tctccaatgt tttaggataa atttccttta gagaaacccc
     5161 tccgccagtt acaaatgatt tctcaatagg aaaagttttg ttgattgata gcttaaactc
     5221 tttgcatagt tgaacaaaat cttgaatttg tttttgagag gcttgttgtc ctgtcagatg
     5281 atccatttca agccgctcaa gaaagaaaac taacaatcgc tcaggcaata taccatgcca
     5341 agcattgacc aagttctttt tggttgcttt acttttttct gtcaatatat gaataagctc
     5401 ttgtttggtt tgtgtaggga aacaatccag agaaagtgtc actggttctc ccgttttctc
     5461 taattcttta ttgacaaaac ttgaacaacg cagtgcagcc ggaccagaaa taccgaaatg
     5521 ggtgaaaagt aaatccatcg tatgttcagt caatacacga ccttttttat ttaaaacgcg
     5581 taaagtgatg tcttgtaaag aaagcccttg aagtgttttc tcctgaatat acggttcatc
     5641 cgatatcaag ggggactctg tcgcataaag aggcgtcacc gtgtgaccca ctcgcttcac
     5701 gatcttgtac ccatctccag tagaaccagt agatggataa gtccgtccac cagttgttaa
     5761 gataatggca ggagcatgga actcttcgaa ttccgtgcgt ataccgtaaa ttttatgttc
     5821 tttgtggatc aatttctcta ctcgagtaga gaatagaagc gtcacatcta gttctttcag
     5881 acgattgagc aatgcttcga taatcgtttt tgatttattt gttacaggaa acatccgtcc
     5941 atgatcctct tcttttaaat gtacgccttg tgattcaaag aagttcatga tatcgaaatt
     6001 attccattgc gcaaacgtac tatataaaaa ctttccattt ccaggaatat gtgcaatcaa
     6061 atcatcaaca ggacgattgt tcgtaacatt gcaccgacca ccacctgtca tcagtagttt
     6121 ttttcctgct tttttgtttt tttcgatcaa gagtacgcgt gcgccttgtt cagctgcact
     6181 gatcgctgcc atcatcccgc ttgttccagc gccgactacg atcacatcat acagtttttc
     6241 cattccctca cctcaaggaa agtgtagcat atctgaagaa agaaaacaga aaaaaatgtt
     6301 ttgagccaaa agagaaaatg agctaaaatg agggaagata agcaaaggag cgtggaacga
     6361 tgacaaacgc aacagaatat gttcaagcaa ttcaagaaaa gctacatcaa aaggatcaag
     6421 ggcaagaaga atttttacaa gcaatcgatg aattcatgcc cacggttatg tcttttttgg
     6481 acactcatcc cgaatatatc gaaaagaata tcttagaact tttaacggaa ccagaaagag
     6541 ttattcaatt tcgagtgcct tggcaagatg atgaaggaaa ctggcgagta aatagagggt
     6601 accgtatcca atacaactca gctatcggcc cttataaagg tggattgcgc ttccatccta
     6661 gtgtcaatct aagtatcttg aaatttttgg cttttgaaca gattttcaag aatagtttaa
     6721 ccggtctgcc aatcggtgga ggaaaaggcg gaagtgactt cgatcctaaa ggaaaatctg
     6781 ataatgagat catgcgtttt tgtcaaagtt tcatgctgga attgtcaaaa catatcggcc
     6841 catcgattga tgttcctgcg ggagatatcg gtgtaggagc tagagaaatc ggctatttgt
     6901 tcggcgaata taaacgattg aaaaaatatg atactggtgt gttgacaggt aaacctttgg
     6961 atttttgggg aagtaagata cgtacggaag ctactggtta tggattagtg tattatgtca
     7021 aacatctatt gaacgaagaa aaagattcat ttaatcaaaa aacagttttt atttccggaa
     7081 gcgggaatgt agccatttat gcaatcgaaa aagtgcaaga gctaggcggg aaagttgtta
     7141 catgttcaga ttctagcggg tacatctatg atccagaagg aatagatgtg aaactattga
     7201 aagaagtcaa agaaatcaat agacaacgtt tgacggaata cgcaaaccaa cgtccttctg
     7261 ctatttatca tgctggagaa tctgtttgga cattgaaaga aaaagcggat atcgctttgc
     7321 catgtgctac tcaaaatgaa atagacgaaa gcttggccga aatcttagta gaaaatggga
     7381 tcaaaatcgt agcagaaggc gctaatatgc catgtacagt aaaagctgtt tctgttcttc
     7441 atgaagcagg cgtgtggtat tgtccaggaa aagctgctaa tgctggagga gtagcagtat
     7501 ctgctttaga aatgagtcag aacgtccagc gattgacttg ggaaagagag caagtggatg
     7561 agcagctgga tcaaatcatg gaaacgattt accatacgtg tcgccaaaca gcagccgagt
     7621 acggcgacga aaaaaatctg cttcttggag caaatgttgc tggatttgaa aaagttgcca
     7681 aagcaatggc agcagaaggt ttagtttgaa aaatgaaact ttcttggaaa tattgtcagc
     7741 attttgttta tttgcttgaa aaataataaa aaaaaacgta aagtattacc tgtagagtaa
     7801 acctataagg aggagaacaa atggcacaca ttcattttga ctattcaaaa gtagcaccgt
     7861 ttgtcaacga acaagagtta gggtacatgc aaagccaagt aacagcagcc cacaacgaat
     7921 tacgggaagg tactggcgca ggaagcgact tccgcggctg gatcgattta ccaacgaact
     7981 atgacaaaga agaatttgca cgtatcaaag aagcagctaa aaaaatccaa tctgattctg
     8041 aagtattagt tgttatcggt atcggtggtt cttatttagg cgctcgtgca gcgatcgact
     8101 tcttgaatca cacattcttc aatttattag atagcaacac aagaaaagca ccacaaatct
     8161 tctttgccgg aaattcaatc agctcaacat atattgctga cttgatcgaa gtaatcggtg
     8221 atcgcgactt ttcagtaaac gttatttcaa aatctggaac aactactgaa ccagcgattg
     8281 cgttccgtgt attcaaagag cttcttgtta agaaatatgg acaagaagaa gcaaacaaac
     8341 gtatctacgc tacaacagat aaagcaaaag gtgcagttaa agtagaagca gatgcagaag
     8401 gttgggaaac tttcgtgatt cctgatgaca tcggcggacg tttctctgta ttaactgctg
     8461 ttggtttgtt accaatcgct gttagtggtg cagatatcga tgcattgatg caaggtgccg
     8521 ctgatgcaag caaagcatac tcaagcgaca aactagaaga aaacgaagct taccaatacg
     8581 cagcaatgcg caacattctt taccgtaaag gcaaagtaac tgagttgttg atcaactatg
     8641 aaccaggaat gcaatacttc tcagaatggt ggaaacaatt gtatggtgaa tctgaaggaa
     8701 aagaccaaaa aggcatctat ccttcaagcg caaacttctc aactgatttg cactctttag
     8761 gacaattcat ccaagaagga cgtcgcaata tttttgaaac agttgtgaag gttgaaaaac
     8821 ctcgtaaatc aatcacaatt cctgaacaag cagaagattt agacggtcta ggctacttgc
     8881 aaggaaaaga agtggacttc gtaaatacaa aagcatttga aggaacattg cttgctcata
     8941 cagacggtga cgtgcctaac ttgctagtca aaattccaga aatggatgct tatacattgg
     9001 gttatacaat gtacttcttt gaaattgctg taggtatttc aggttacttg aatggaatca
     9061 acccattcga ccaaccaggt gtagaagctt acaagaaaaa catgttcgca cttctaggca
     9121 aaccaggctt tgaagatctt gctaaagaat tgaacgaacg cctgtaagat aagggttttt
     9181 cgctagattt aaataatcag tgatagaaac atgttcgctt taaaatgaaa actggtaccc
     9241 atttaggtac tcaaacatac tgcatcgttt attcagaata aggggctgtg acaagacgtt
     9301 tgtcacagtc ccttgtttgt atagaaatcg attgattgcc ttgctcttta gtttgtttta
     9361 ggactttaag aacagtgagg ctgacttcga aggttaattc tgtttttttg ttttctagaa
     9421 tcagtaagca aaaaagcact cacttcgagt gcttttttgc gatatattct tagcttaaga
     9481 taggcctaat ttttctttta cagatgaggg taccttatcg ctggtaatta cttttttctc
     9541 tatgatccgc ttttcgcttg ctttcaactg caagatcgtt ccttcggggt aagcagaacc
     9601 attggcagag tcaccagaag taatgacatc gaaatctaac tgtctttttt caccgttttc
     9661 agcataagca tctacgttgt atttaaaacc tcttccaatt acttccccat tatctgcttt
     9721 gatatcagtt tctgcaggca ttggagcttt gactaccgca taataatctt tacctacata
     9781 agtagactga tagtagtccc aacctttcca tcctataaaa gcaatcaata tgataacgat
     9841 caaccctaaa atttttttca tgaaagatct ccttttttat ttttatta
//
LOCUS       DACCYH010000100         9805 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.105, whole genome shotgun sequence.
ACCESSION   DACCYH010000100 DACCYH010000000
VERSION     DACCYH010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.105"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(193..1041)
                     /locus_tag="IVY85_002474"
     CDS             complement(193..1041)
                     /locus_tag="IVY85_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8045444.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1205..2107
                     /gene="gnd"
                     /locus_tag="IVY85_002475"
     CDS             1205..2107
                     /gene="gnd"
                     /locus_tag="IVY85_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP8045445.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2109..3110
                     /locus_tag="IVY85_002476"
     CDS             2109..3110
                     /locus_tag="IVY85_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8045446.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3107..3391
                     /locus_tag="IVY85_002477"
     CDS             3107..3391
                     /locus_tag="IVY85_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8045447.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3424..4221
                     /locus_tag="IVY85_002478"
     CDS             3424..4221
                     /locus_tag="IVY85_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8045448.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4235..5068
                     /locus_tag="IVY85_002479"
     CDS             4235..5068
                     /locus_tag="IVY85_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8045449.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5086..5943
                     /locus_tag="IVY85_002480"
     CDS             5086..5943
                     /locus_tag="IVY85_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8045450.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5965..6474
                     /locus_tag="IVY85_002481"
     CDS             5965..6474
                     /locus_tag="IVY85_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8045451.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6511..6912
                     /locus_tag="IVY85_002482"
     CDS             6511..6912
                     /locus_tag="IVY85_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045452.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7024..7377
                     /locus_tag="IVY85_002483"
     CDS             7024..7377
                     /locus_tag="IVY85_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045453.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7411..7854
                     /locus_tag="IVY85_002484"
     CDS             7411..7854
                     /locus_tag="IVY85_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8045454.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7871..8956
                     /gene="uxuA"
                     /locus_tag="IVY85_002485"
     CDS             7871..8956
                     /gene="uxuA"
                     /locus_tag="IVY85_002485"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8045455.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8949..9662
                     /locus_tag="IVY85_002486"
     CDS             8949..9662
                     /locus_tag="IVY85_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8045456.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         3257 a         1555 c         2166 g         2827 t
ORIGIN      
        1 actttgttat cgcatctaac aagtaactcg aataatacca atacaacctt ctccaaagat
       61 gcgatgacat acgttctaac ccaccgacta ttgatgttct gtaccaccct tgcagaacca
      121 aaaaagccac taccaaatat ttggtagtgg ctttttttgt tttatggatt tttcgctttt
      181 tctgttatgg tgctattctt tgatttcaga aatagtatgt cgaactttag cgatagaacg
      241 acgtgattct ttttcgttgt aaaacatcaa aatcacatat agagagtcca aaatgcttag
      301 ttgagaaata cgggaagcta aagcttcaga tcggaattct gtttcttcag aaatagaaat
      361 aaaaacgaca tcgccaagtt tagcaaggga agaattagct tggctagtaa taacgattgt
      421 ttttgctcca gtttttctta cggtttcagc aatatggatt gtttcttttg attgccctgt
      481 gtgagaaatc aaaacagcac agtctttttc cgtcaataaa gaagcttcca tcaattgaat
      541 atggtaatcc gtgctgtgag aagttgcgat aggggaacgc aaaaatttat gataagcgtc
      601 agttgctaca atctctgaac cgccaacacc gaaaaaatag acacgtttag attcattgat
      661 aatagtagcc gcttttttta gatccttttg ttgtaacaac ttttttgtgt catttaaagt
      721 tgaaatacta gactcgaaca ccttttgcgc catagtgagc tcagtatctg ttttctgaat
      781 attttcgtgg atagaaatag cagatagatc attttcttgg atcaacatgg ccattttaaa
      841 atctttaaat ccattataac caagtttttt tgtaaattga aaaaaagtag agtcagcaat
      901 gcccagttca cttgctaaat tgctaatagg actatgactt actttgcttg gattttctaa
      961 aatataatcg gcgatagcct gttcttttgg gctgaagtcg ttgtacattg ttttgatttg
     1021 tagtcttatg gagacgctca tgagaattcc tcattttcat aataagatta tcattttttc
     1081 cgttctcatt aatgttaaag atacaaggag agtaggaaaa aatcaaacaa aaaatatttt
     1141 tgtaaaatct atttacaaaa aaattttttt gtataatgag ggtataagaa atggaggcgc
     1201 tatcatggaa attggtgtaa ttggtttggg aaaaatggga ttgaatttag ctttaaactt
     1261 aaaagagcac catcacaaag tacaaggact cgacatttca agtacagctg ttagtcaggc
     1321 aagagaaaat gacattcctg cttatcaaga gatctctgaa tttctagcta cttttgagca
     1381 gaaaaaaatc atctggttaa tgcttcctgc tggagagata accgaaaaca tgttaagaaa
     1441 actttttcca aaactcggac caggagacat tattattgaa ggcggaaact cttattataa
     1501 agattcaatc cgtcgagcag aagagtttca gaaaaatgaa atcggttact tggattgtgg
     1561 gacttctgga ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg gaggagataa
     1621 agaaactttt ctatccgtgg aacaattatt caaagatgta gcaattgaaa gaggatatct
     1681 gtatgctggt gcatccggaa gcggacactt cttgaaaatg atccataatg gaatcgaata
     1741 tggaatgatg caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt
     1801 tgacttggaa caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg gctggctgat
     1861 ggaaatcatg gaagatcagt taaacgaaca tcccaattta agtaattatc ggggaatcgt
     1921 ttctgcatca ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg ctgttccagt
     1981 tccagtaatt gcaactagtt tgttcatgag gaatctttca caggaagaag atagtttttc
     2041 agcaaaagta gtttcagcac taagaaatgg gtttggcgga catgaaatag ttgaaaaggg
     2101 tgagtaaaat gccagattat catatagcaa tcgttaattc cagcagtttc ggaagagtct
     2161 ttccacacca cttggaaaga ttaaagtcag ttggagacgt tcatttcttt actgttgatc
     2221 aagagattcc aggaaaagaa ctagcggagt tacttaaagg attcaatatc attattgcaa
     2281 gtgttacgcc attttttcga cgagattttt ttgaaaacaa agatgaactg ctattgattt
     2341 cccgacatgg tattggttac aacaatattg atttaaaagc agcaaaagaa caccagacaa
     2401 tcgtatctat catacctgct ttaattgaac gaaatgctgt agcagaaaat aatataacta
     2461 atttgttgtc catgcttcga cgcacgacac aagcacaagc aagagtcaag gacgatcgat
     2521 gggaagatcg ggcggagttc gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg
     2581 gtgcaggaaa taccggaagc ggagtaatcg aaattttgcg taacggtttt cgctgtgatg
     2641 ttctagctta tgatccttac aaatcagatt tatacatcga aagctttggt gcaaaaaaag
     2701 tagaacttca agaattactg gaacaatcag atatcatttg tttatgtgcg aatttgacag
     2761 aagagaacta tcacatgatt tctgataaag aagtcgatca gatgaaagaa aatgtttatc
     2821 tatctaatag tgcaagaggc gctctagttg atgaagaagc gatgattaat ggtctgaaaa
     2881 gcggaaagat cgcaggatat gccacagatg ttttagaaga agaaccggga cgtcaagatc
     2941 atccgtattt agcgtttgaa caagtagtga ttacgccaca tatatcggcc tacacaaggg
     3001 aatgtttaga agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc
     3061 ttcttcctga acgttctgtc cagacaaaga gcaactatat aaaaggatga tgaagaaaat
     3121 ggtgaacggt ttaaaagtga aaacaggccc tcaattttat ttatatgaag aaggtggtat
     3181 ttcaaaagtt tctgatctct tgaaatcata cggcgcgaaa agagtactgg tcacccacgg
     3241 aacggtatca tgggaaaagg cgctacctaa attagtattt ttgaatgacg aaacgattca
     3301 atttttttat catcgttatt ctggagaatg tagttatgct gaagccagac gaatagctac
     3361 aatcatcaaa aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa actgaccgat
     3421 ctagtgggtt atagtgccaa tcttgcaaga atcgatttcg gagtcatccc tactttagct
     3481 agtaattgtg ctccatggac acctcttgct gtcatgtata aggagaatgg acaagcagaa
     3541 gggaaaacag aacactttta cagacaagct gcttttttta taacagatcc ccaattagtg
     3601 attgattccc ctgttccata tttcattgcc gggttagcag atacactagc aaaatggtat
     3661 gaatctgagc tcatcttaga acaagatttt ttgcaagatg aatcattttt gaagctagca
     3721 caagatactg cgaagatctg caaagaagag attttgaatc attctgctaa agcaatccaa
     3781 gatatggcac aaagaaaact gacatcggag ttcaaacagc tttcagagat tatctttgct
     3841 gttgctggtt tagttggagg attaggagat aaatacgcaa gaaatgcggc agcacatgct
     3901 atgcatgacg ctatcagtaa atatattccg gaaagccatc gttttctaca tggagaaaaa
     3961 gtagcgtatg ggattttcta tcaactggca ctagaaggtc gctgggaaaa aatagacgag
     4021 ttatatcctt tgtatcggga attagaactg ccggtttctc tagaacaaat gggattattt
     4081 ccgaaagata aacagataat agataaaatg gctgaattta ttgattcgaa agagaaagtg
     4141 catttgattc ctatagaggt aacacagaaa tcactgatac agtcaatgat ggaactggaa
     4201 ggttatatca gaaagatctg aaggaggagg acacatggaa acattaagtg tagtacaaag
     4261 tttgttgatt gctttatggg tagccgcggt tatgtcccgc tggttaggtg gaggagccac
     4321 gttgacctta cgtttttctc ctttgatgac tggattagtc gtcgggttga tcatggggga
     4381 agttgcacaa gcgatgattg ttacagccgc tttgcaaatg atttatatgg gggttttttc
     4441 tccaggagga tcaatgccag ctgaaccatc tattgcagca gctatcgcag taccagtcgc
     4501 attattaggg gatctgtcgc cagaagcagc gattgccgtt gcggtgccag tcggattgct
     4561 cggaagttac ttgtatcagt ttcgtttttt catcaatact tttttaggta aatatacaga
     4621 caaagctgta gaagatttga atacaaaagc tatggcccga tcaattattc tttatccaac
     4681 ggttgcctca tttttattat tcgttccgct tgttttcatt gctttatatt taggggcgcc
     4741 attgatcgct gatgtaatca aagctttaga gggaacagtc gttatccaca ttcttgaagt
     4801 agtaggtgga gggttagcgg caatcggtat cgcgacaact gtatacgtta tcggtcgaaa
     4861 agattatctg gtctttttct tccttgctta ttttatgagt gttgttttga aatctttaga
     4921 tattacgatg gttacttatg ccatttttgg gattattatc gctttgatct ttgtccagtc
     4981 gcaaaaaggg aaacaagtac agcctgccac aagcaataac aattcagcat cttttgatga
     5041 cgaagatgac tatgatgacg gattttaatt taggaggaat gggaaatggc tgaaataatg
     5101 gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa agcctatctg
     5161 agatggcatt ttgcaaacga aattcctcac tcttttgaac gttacttggc gccatcacta
     5221 ctatacggaa tgatgccaat cttgaaaaaa ctttataaag atgaagactc tttaaaagca
     5281 gcatataaac ggcagttact gttcttcaac actcaattaa gctggggtgg cggtgtgatt
     5341 actggcctaa tgtcttctat ggaacagcaa agagcagaag aagagtatga agaaaaagag
     5401 atcttgatgc aggatgactt gatgtataac acgaaagcgg gattgatggg ggcactagca
     5461 ggaatcggag atgcaatcga ttcaggaacc gtccaatata tttttatcgc aatcgcggta
     5521 ccttgggcac aacagggaag tcctttaggc gcactttttc cttttatctg ctttgggttg
     5581 tatcaggttt tactaggggt atttttcgca agacaagctt tcactatggg ccgtaatgct
     5641 acaggcctaa tgcaaaacac tggtgttcag acagcgattg aaatgctatc agttttaggg
     5701 ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc attgaaattt
     5761 gcgatatccg gacgtgagtt tgttatccag gaaattcttg atcaaatcgt gccagggata
     5821 ttacctttag ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg attgaaagtc
     5881 acacaagcat tattatggct gactggaata ttaattgtgc tagcaggaat cggtatttta
     5941 taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt ggatgagcgt
     6001 ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa ttccattttt
     6061 gttgtagacg aagttgtagc aaaagataaa tttatgagag atctttacaa aaatgcagga
     6121 agccgtacag gacaaaaaac aattgtcatt acaccagaga aagctaaatt ctattgggat
     6181 gaatatcaat ttaaagaata caattgtatt ttgatcgcaa aaacagtcca aacgatttac
     6241 gacttagtaa aacacggaat ccccatggaa gaattgaata ttggagggat tgctcaaaaa
     6301 aatcctgata cagatctttt agtgacgaaa tctgtttatt taaataaatc agatgctgaa
     6361 aaattgaaag aaatgaatga acaatatggc gtaaagaata tttattttca agccactcca
     6421 tctgcaccta aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc ataaacaaat
     6481 gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga acgagctgtt tcgtgattgg
     6541 gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa acagcgtttt
     6601 ctttcagcat tagtatcaga tatttacagt atgagaacag atgtgacagt cattgcttac
     6661 gatacgctag cctaccgatc aaaaaatatt tatgttggag atatcgaaaa agcagagaaa
     6721 gttatctgta cttattacga tacacccgtt catgcattag gaagttattt catgtttgat
     6781 tggaaagacc aaaggaaaaa aacaatctac tctatattat tgtccttcat tttgttgttc
     6841 agtttaggtt ggtggggaat gatgatatat aacaaaaatc ctcatcacgt attcgatctg
     6901 ctatccgtat aaacaggtat cactgtacta gcatttggga gctacttttt cttattggga
     6961 aaagcagctc gcggatggcc tagcagacaa acttttattc gaaatacttc atccatatta
     7021 acgatgctag aaatgatccg tacgattgat gatccgagtg tagcgtatgc gtttgttgat
     7081 gaaggctgtt atggagaaaa gggcttagac agcgtgagat cgggtatgaa aaaagaggct
     7141 attctttttt atctagactc tgttggggca gatacaccgc tacagttttc gggaaattat
     7201 ttttcaaata aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa aaacgtcaat
     7261 tatattttta gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa aacagattta
     7321 cgagggaaaa catttaattg gcaaaatgcc aatcagatta ttgctctttt tcgataagta
     7381 aaaagtgcaa aacaaggagt gagaagacta atgttggaaa tcatgatcgt tacacatggt
     7441 aagttgagcg aaggactgaa agatgcggcg gaagtaattg tcgggacatc gagccatatt
     7501 gaaacaatgt gcttagagtc tggggaggac atccaaacat tcggagagaa ggtcaaagaa
     7561 aaattaacta tgatcaacac agcagaaccc atgctagtat ttgttgatct aattagcgca
     7621 agtccgtaca atcaagcgct cttggcagtc aatgaactga ctgaagaaaa gaaagcagcc
     7681 acttatgtca tagggggcgt gaatctgcca atgttgttag aagcaatcaa tcatcagatg
     7741 cttcacacac cgatagaaga agcggtggaa caaattgtca aacaaggaaa agataattta
     7801 actgtttggc atgcttcagc ggaagaaaaa aaattagatg aggacgattt ttagaaggaa
     7861 ggctgaaaag atgaagtggg gatttagatg gtatggtgaa aaaggggatt cgatcccatt
     7921 aaaatatgtt cgccaaatac cgggaataaa tgggataatt ggaactttgc tgactaaaat
     7981 gcctggagat gtttgggaga tttcagaaat tgaggagttg aaagcatctg ttgagagcca
     8041 aggactggaa ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca aggcagggac
     8101 agatgaacga gattactata ttgataacta tatacgaaca atcaaaaact tgtctcgatg
     8161 tggggttcga ttgatttgtt atagcttcaa accaattttt ggatgggcga aaactgattt
     8221 gtttaatgaa aacaacgatg gaagctattc tcttttatac gatcagaaaa tcgtcgatca
     8281 gatggagcca ggagaaatgt atcaattgat acacagccag tcaaagggat tcaaattgcc
     8341 gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt atgaaggaat
     8401 gaccgaagaa gaattatttg aaaatctgac ttatttttta gaaaaagtga ttccagtgtg
     8461 cgaaaaagca gatgtaaaaa tggctatcca tccagatgat ccgccatggg aactttttgg
     8521 ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa attctaaatg cagtagattc
     8581 tccatataat ggactaacgc tgtgcacagg atctttaggg gcaaatccgg aaaacgattt
     8641 agttgaaatc atccatgagg taggttcgcg catccatttt gtccacttca gaaacgtgga
     8701 gtttttagga gaaagaaagt tcaaagaaac ggcacatccc tctacagaag gctcccttga
     8761 tatgtatcat attatgaaag cgttggtgca agaagggttc gacggtccta tacgccctga
     8821 tcatggtaga accgtgtggg gtgaagtggc tatgcctgga tatggcctgt atgatcgtgc
     8881 aatgggactc acttacatgc aaggtctata tgaggcgata agtaaggaaa aaccactggg
     8941 agagcattat gaataaaatc aagctcttca tctttgatat ggatggcctt ttattagaaa
     9001 ctggacgttt agcttatcgt gcttatgtaa aatcagcaca aaagtatgat tatgaaatgc
     9061 gtaaggaagt ctattattta ctaactggcc aaacagaaat ggcgataaga gaacaaatgg
     9121 gcattttgta cggagaagat gttccatata tcaaatggcg agaagcaatc aatcaatata
     9181 aagaaaaaat cgtgaaagaa gataagagag tatatacgaa aaaaggagca gaagaaattt
     9241 tatcttttgc aaaagaaaga ggaatccata caatcgtggc ttcgtctaat acacgggtaa
     9301 aagtagaaat gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa atcatttcag
     9361 gggatgatgt aaaaaaagga aagccggaac cagagatctt tttgaaagct tgttcaaaaa
     9421 tgaatattcc tccaagtgaa gcacttgtat ttgaagactc gattgcaggg atcgaagcag
     9481 caaggcgagc gggcattttg tcttttctca tcaaagatca tttgataggg ttgcccgatc
     9541 ataatgggaa gcataagttg aaagtagatg taagtttata tgaatccaat aaagcagact
     9601 atgaatttta tgacctgcat caagcaaaac gatttttaga aagtaaaggg ttgtatctct
     9661 aataaagagg actattttct agtcctcttt atttattgtc ctgcacgact gagggaacag
     9721 atgaaaaaga agtaattttt tattttgaag tatctacctt atttgtagtg aacaatttag
     9781 aacaaacaca aaacgcatct actgt
//
LOCUS       DACCYH010000101         9716 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.106, whole genome shotgun sequence.
ACCESSION   DACCYH010000101 DACCYH010000000
VERSION     DACCYH010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.106"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            21..644
                     /locus_tag="IVY85_002487"
     CDS             21..644
                     /locus_tag="IVY85_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045457.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1094..2143
                     /locus_tag="IVY85_002488"
     CDS             1094..2143
                     /locus_tag="IVY85_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8045458.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2140..3021
                     /locus_tag="IVY85_002489"
     CDS             2140..3021
                     /locus_tag="IVY85_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8045459.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3024..3794
                     /locus_tag="IVY85_002490"
     CDS             3024..3794
                     /locus_tag="IVY85_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8045460.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4073..4927
                     /gene="aroE"
                     /locus_tag="IVY85_002491"
     CDS             4073..4927
                     /gene="aroE"
                     /locus_tag="IVY85_002491"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP8045461.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5105..6268
                     /locus_tag="IVY85_002492"
     CDS             5105..6268
                     /locus_tag="IVY85_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP8045462.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6385..6795
                     /locus_tag="IVY85_002493"
     CDS             6385..6795
                     /locus_tag="IVY85_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8045463.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6969..9197
                     /gene="topB"
                     /locus_tag="IVY85_002494"
     CDS             6969..9197
                     /gene="topB"
                     /locus_tag="IVY85_002494"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8045464.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9335..9652
                     /locus_tag="IVY85_002495"
     CDS             9335..9652
                     /locus_tag="IVY85_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045465.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
BASE COUNT         3366 a         1648 c         2088 g         2614 t
ORIGIN      
        1 gagaaaaagt agaaaagaag gtgtttgtta tttgggaagt agttaaatcg agagtacaag
       61 aaatgaatga acatttctca gtgtcattcg cagaaaatcc aagtaagtat tttcgacgtc
      121 ttgtttttct gctcatctta tcattgctgt taacagttgc aatgttttta ttgccggaaa
      181 aaatccgaga agagcaagta aatagtctcg tttattcgtc cataccaacc agcaaattga
      241 aaccttttga ttacagcaaa gctgatcaga aaatcaaaca aagccgggcg atatcggtga
      301 tgttcagcaa accgaacggg aaaacctatc aggacatgct tgatgttttt aatgatcctg
      361 agcttatgga agaattgaat cgaccgatct tttattatcc gatcgtttat gatgtacatg
      421 aactggaaca aaaatataac atacgaacgg atgaagtgac atttattttc tttgatggag
      481 gaaaagaagc aaatcggata actgcgggta agggaggaat aactgatttt gataaggagt
      541 tgatccctga actgaatcga cttccgttag ccaatatcaa acaattagaa gaagaattgg
      601 atagaacggg agtaacttcc caaaattccg agattgctaa ttgagacatt ggcgcgatga
      661 aacggtgggt ctatatagcg ctatttttaa ttatcagaac aagtgatgtt tgatcttcaa
      721 aataacaaat aaaaaattaa aaaatatagt tgacgaaatt caaaaattcg attagactta
      781 aatcaattct taaggaaagg gtgacctgta atgaacttat caaaacgtat ttcagcagaa
      841 aacaaaaaaa attggcgcag ctggcgtaga taagttgtat taatggtgag actttcccat
      901 agatacaact ttttagagtg catttctaaa gcttgtatct atgctagaca tgaacattga
      961 ctgccgcata gattagaatc tatgcggttt tttgtgtaaa gacaatgacc gctttggcca
     1021 ttgtcttttt ttgtagactg gatacttgcc tgattataat aaataaaaag attgactttg
     1081 caggaggaaa aaaatgaaaa acaaacgatt aataacagtt gtagtaatta ttttagttta
     1141 tttagcaggt gcatttattt atggaaaagc ctcaggcgaa aaagaaagta cttcgacgaa
     1201 ccagacaaaa aaaacaacag ttgttggggt attgcaatac gtgagccatc cggcattaga
     1261 tgagatttat cgaggaatca aagatggtct gaaagaagaa ggattaaaag aaggagaaaa
     1321 tcttgagatc aaatttcaaa atggacaagc cgatcagagc aagctagcca caatgagtca
     1381 acagctagta caatctgatc cagatgcctt gattggaatc gcaacacctg ccgctcaatc
     1441 attagcaaac acgacttcat cgatcccagt tgtgttaggt gctgtaacgg atcctgtggg
     1501 ggctggatta gtcgcttctt tagagaaacc tggtggtaat gtaacaggtg tttctgatca
     1561 gccgcctgtt acttcacaaa tcaagcttgc ttctgatttg ttgccaaatg caaaaaaagt
     1621 cggaatcttg tatgcttctt cagaggataa ttcaaaatac caagtagcac aagcagaaga
     1681 agctgcaaaa aaagaagggt tagaatcagt aaaatatgcg gtgccttcca ctaatgagat
     1741 tgcacaaacg gttcaagtga tgagccgaca agtcgatttt atctatgttc cattagataa
     1801 taccattgcc aatgcgatgc aaacagtggt aaaagaagca aacaaagcaa atatccccgt
     1861 cattccatct gtggatacaa tggtggaaca aggtggacta gcaacaatcg ggatcaatca
     1921 atatcaatta gggcttcaaa gcggtaagat ggcggcagaa cttgcgagtg gaaaagaaaa
     1981 accggaaacg acgcctgttt atacatttga tcaaggggat acagtgatca atcaatctca
     2041 agctgatcgc ttaggaatca ttgttcccca atcgatgaag gaaaaagcca aaatcattac
     2101 agatgactcg cagcaagaaa catctaagga ggatgacaaa tgatcgtatc agctatttca
     2161 caaggcatgc tttgggcaat attaggatta gggattttca tgacttatcg gatcttagac
     2221 tttcctgata tgacgacaga agggtccttc ccattaggtg ctgctgtctg tgtgacagct
     2281 attactcatg gggtagcgcc gattgtagct acattattag gcgtaggtgc tggcatgata
     2341 gcaggactcg ttacggggtt actttataca aaaggaaaga taccggttat tttagcaggg
     2401 attttagtga tgtcaggatt gaactcagtt attttgtttg tgatgcgaac accaaatctt
     2461 tctttattga atcatccgat tctacaagac agcttccatc gctttgggtt acctgattac
     2521 tttgacacag tccttcttgg cttcatctct ttgggcatct tgattgctgt actgctattc
     2581 ttcttcaata cagatttggg tcaaggctat attgcgacag gagacaacga aacgatggct
     2641 cggtcgttag ggataaaaac cgatcgtatg aaaattttag gtttaacttt gtcgaacggt
     2701 gtcattgcat tatccggtgc gctgattgcc cagaacgacg gatatgcaga tgtaaataaa
     2761 gggattggcg taatcgtcat cggtcttgct tccatcatca tcggtgaagt gatcttccaa
     2821 gaattgacgc tagcagaaag attgattgct attgttgtcg gcagtatcat ttatcagcta
     2881 ttgattttag tagtaatcaa actagggttt gacacgactt atctgaagtt gttctccgct
     2941 atcatcttag ctatttgtct gatgatcccg caattgaaga aagcactgaa cttaaaaacg
     3001 ggttttgaga aggaggcata gtaatgactg atatattgac aatcaaaaat ggcagaaaag
     3061 tcgtaaacaa tggaatgaat gaagagaaag tactattgga tgaaattaac cttacaatca
     3121 gagaagggga cttcatcact gtccttggcg ggaatggtgc tggaaaaagc acactgttca
     3181 atacgatcgc tggtacgctt caactcagtc aaggatcgat ctcttttaaa gatcagctga
     3241 tcacaaaaga ttcagaagaa aaaagagcac gattcctttc tcgagttttc caagacccca
     3301 agatgggaac ggctcctcgg atgacagtag cagaaaattt gctacttgca caaaaaagag
     3361 gtcaaaaacg gaaattccgt ttacgtaact tgaaaaatca aaaagaagat tttttcagac
     3421 tatgtcaaga agtcggtaat ggcctagagc aacacttgga tactccagcg ggtgaattat
     3481 ctggtggtca acgccaagca ttaagcttgc tgatggcgac gatcacagct cctgagttac
     3541 tattattaga tgaacatact gcagctttag atcctaaaac agctaaagca ttaatgcgat
     3601 taacaaatca gcggatcaat gaacaaaaat tgacttgtct catgatcacg catcgaatgg
     3661 aagacgcctt gaaatacgga aatcgtctga tcgtactcca aaaagggaaa atcgttcaag
     3721 atatttctga aaaagaaaag caacatttga caatgggcga ccttttgcag ttctttgaag
     3781 aagtagtgga ttgatttcga cagaaaaatt cctttcagta gaaggacttg aaaactcatt
     3841 gacatcttcc tttgattcat cctatactta aaacaataaa caattaaaca tatttgggtg
     3901 ttttgtagga tcacgtagtc aatgatttta acaattaaat agaatattct ttgctctgac
     3961 aagtaaacga tgagaaaaga atttgctact ttgccaccta gacgtttcgt actttttcgt
     4021 atcaaacgct aagtggcttt ttttattgaa gtaagaacaa aggaggaata agatgatttc
     4081 tggacaaacc aaattagctg ggctctttgc tgatccggct tctcacagtt tatccccatt
     4141 gatgcacaat actgcttttg aagcaaacaa catcgatgcg gtgtatttag ctttccaagt
     4201 aaatccaatg aatttgaagc aagcagtcga aagtattcga acttttgata tgttaggtgt
     4261 aaatctttcg atgccgaata aaacagcggt tattccttat ctggatgagg taagccagga
     4321 agcgcaattg atcggagcag tgaatacgat cgtgcacgaa aagggtcgtt tgattggcta
     4381 taataccgat ggtgcgggct tcatgcaatc cgtgcaagaa gctgggattt cgatcaaaaa
     4441 gaaaaaaatg actctactgg gagctggagg tgcagcaaag gcagtcactg ttcaagctgc
     4501 gttagacggc gctgaggaaa tcgtagttta taaacgaaac aaccaaacat tcgctcaagt
     4561 aaaagaaaca ttcgaacaac tcagctatcg aacttcttgt aagataaaag ttcgtgactt
     4621 tgccgatacg catcaattaa gaaaagactt gaaagaaagc tgtcttctag tcaatgcaac
     4681 agatatcggg atgggaaaga aaatcaatca gtcaccgctc ttagatataa gcatgatgca
     4741 tgagaatcta gcggtttttg atttgattta tgcacctaga gaaacacgtt tattaaaaga
     4801 agcaaaaaaa gtaggagcaa aaggatacaa tggacttggc atgttgatct accaaggagc
     4861 aatcgcgttt gaattatgga caaaggaaaa gatgccgatc catgtgattg aaaagctgtt
     4921 tgattaaaaa aggataatag ataaagatct tttattctcg cataaacggt ctcaagaaaa
     4981 gtaatccttt atgtatcgaa gcatttattt cgttattttt tattaaatta tcaaaaaatg
     5041 ataggctttt gtcgattggt tatcgtatcc tttatttatc agtaagaaac gaggaggaac
     5101 cacaatgagt cggatggatc gctataagga catccataag aaggcgaaac ctctgaagcg
     5161 ggatacgggg agtatttttg ctagaagaga acgaaaacga acgcgtatgg aaaatgagga
     5221 agatgctacc cgtatttatg aaagagagca gcattcattt gaacatttga accaatacga
     5281 gcaacagata tacagagatg caccaagagc agaagaacaa caaaaaaata aaaaattttt
     5341 caatcggcaa aagaagaaca aaccgttaaa acctcgaaga aagcgatcgt ggaaaaaaat
     5401 cattctatcg attttgcttg ttttgatcct ttattcaggt atctcatttt ttattggtaa
     5461 atatgtagct gaacatgaca caagcttaga ggcagcgact tcagaaacct ttcatgggca
     5521 aaaagcgacg aatggtgcgc ataatatttt gatccttgga agtgatacac gcggagaaga
     5581 tgccgggcga gccgatacga tcatggtctt acagttggat ggtccttccc ataagccgaa
     5641 actgatttct tttatgcgtg acagcttcgt tacgatccca ggggtaggac aaaataagat
     5701 caattcagct tatgcatacg gaggagccga tctagtgcgt caaactttag tagaaaattt
     5761 tggaatcgat tgtcaatact atgcgaaagt cgatttcaaa tcgtttgaaa aagtgatcga
     5821 tgccttattc atgaacggag tgaagatcga tgcagaaaaa gatctgaatc tggatggtgt
     5881 agatattaaa aaaggggtcc agaagatgga tggacatgtt cttttgcagt acgcacgttt
     5941 ccgaatggat gaacaaggag actttggccg tgtcagacgc caacagcaag taatgaacgc
     6001 tattttcagc caattgaaga atccattgaa tctgatccgt gcaccttatg cagctggtaa
     6061 agctttagga tatacatcca cagatgtcac tgcttggttc ttgacgaaaa acctcttttc
     6121 gattgcacga ggagctggtg gcattgatcg aataagtgtg ccagtagacg gttcgtggaa
     6181 ttatgggcaa agtgattatg ctggcagtat tcttgaaatt gataatcaaa tgaatcaatc
     6241 tgctattaca gattttttga ataaatgatc ataaaaaaga tcaagaagaa gctgcgcaag
     6301 gcttttgtca tgaatcaatc aaacgattca ctcgtaaaaa acgctttctt atggtagatt
     6361 taaccaagag aggagctgtt tacattggat atctatctga acttcaaaaa tgagtcgcgt
     6421 gaagccatcg ctttttatga aaaagttttt cagactgaag catcaggtat caccacatac
     6481 ggtgaaatgc ctggaataga acaaatgaac ttgccagaag aagcaaaaaa tctaatcacg
     6541 aatgcacggc tgaacattta tgatacatta gttatgtttg cagatacgcc agaaggaatt
     6601 ggtcctgaat tagttgtggg aaataatgtt tctttagtca tccagacaag aaatgaagaa
     6661 gaaacgaagc gcctatttga aagcttagct gagggtggta agattgagat gccactggat
     6721 gctgttccgt gggctcagtt gtatgggatg gtaactgatc gatttggcat caactggcag
     6781 attaaccaag agtgacagct aaagatgaat attttctgga aacatcaagc agcaaacgga
     6841 tcatcgtttg tttgcttgat gtttttgtgt ctatttcgac tatacggtat ctttttttta
     6901 ttgccagatg cgaaaatatg ttccataata gagaagtccg caaaaaatta agtaacggag
     6961 gtcagaagat gaagacggtg atactagctg aaaaaccttc acaagcaaaa gcctatgccg
     7021 aaaccttcca acagcaaaag cggcatgaag ggtattatga aatcagcgat ccattgtttg
     7081 ctggtgaagt aaccatcact tatggttttg gacatttggt agatatggtt gctccaggag
     7141 cttatgaaga gcgttgggca aaatggtctt tagaaaattt gcctattttc ccagaaacct
     7201 tccgttacga agttccaatg gacaaacgtg cacaattttc gatcgtaaaa cgagaattgc
     7261 agtcagccga taccatcatt attgcaacag atggtgatcg agaaggtgaa gccatcgcgt
     7321 ggtcgattat tcgacaagca aacgcatttt ctaaggaaaa gaactattta cgtttatgga
     7381 tcaattctct tgaaaaagaa gcaatctatg aaggttttaa gaatctccaa cctggaaaga
     7441 actattttcc aaaatataaa gaagcgcaag caaggcagaa cgctgattgg ctgattggaa
     7501 tgaatggaag tccattatat agcttgctac ttcaacgaaa agggattgat ggcagttttt
     7561 ctcttggtcg tgtacaaaca ccgacactct atatgattta tcagctgcaa gaagaaatca
     7621 aacacttcaa aaaagaaacg tactttgaag gagaaggttt aattacgaca aacaaagggc
     7681 agttttctgg aaaaatcgta ccaaaaaaag catttaaaac ccaagaagag ttaacgaagg
     7741 aaatagaaaa acttggagcg catttaggta agcaaaacgg acggattctt gaagttacga
     7801 aaaaggaaaa gaggttgcat agtccacgtt tattttcgct atctagtttg cagacaaaga
     7861 tgaatcagct aatgaaagca agtgccaaag ctacacttga tgctgctcaa ggattgtacg
     7921 aaagcaagtt tctaagctat ccaagaacgg attcctttta cattacagaa aatgagcatc
     7981 agtatctggt taaacatctg caaaattata aaaaattttt aggaatagaa gaagtacaga
     8041 caactgagat caaagcaaag aaacgatatg tcgatgcgaa aaaagttcaa gaacatcatg
     8101 caattattcc gacaaagacg attccgactc ctgcacgctt tgcgaagctt tccaaattac
     8161 agcaagcaat ctatttgcaa gttttgcgca ctactgtggc tatgtttgct gcagattata
     8221 tctacgaaga gacgaccgta atgacgggcg tacagcaatt aaagctaaaa agcatcggaa
     8281 agatcccatt acagcaaggt tggcaggaaa tcttacgcat caagtcaaaa aagaaagaag
     8341 ctcagactct tcctcctatc gtagaaaatg aagaagcaga agtagaactg aaaagtatgg
     8401 agaaagaaac acaacctcca aaaccctata ccgaaggaac gttgatcaca gcaatgaaaa
     8461 ctgcgggaaa aacgctggat aatgatgaag cgcaagaaat tctcaaagaa gtagaaggaa
     8521 tagggacaga agctacacga gccaatatca ttgaaaatct taagcagaag cattacatcg
     8581 aagtcaataa aaacgaaata actgttacgc caaaaggaat cactctttgt aaggcagtag
     8641 ccaatgaacc attactgacg agcgcagaaa tgactgcgcg ctgggaaggg tacttgagaa
     8701 aaattggtaa acaagagggg acaccatctg tttttttaga gaatatcaaa aaattcatcc
     8761 tacatttatt ggcagaagtt cctaatcaaa tcgacaaggt agacttttca gatgagatta
     8821 gcgggacacg tcagataaaa gcgatggaga agaaaaacga tcaattagga agatgtccaa
     8881 aatgtaaaaa gggaatagtc atgctttatc ctaaaatcgc tacatgcttg aatccggaat
     8941 gcgacttcaa attgtggcca acagtagcta aaaagaagct gactaaaacg aatctcagag
     9001 atttgctttc aaaaggaaag acaagtaaag ttgtcaaagg ttttactggt aaaaaaggga
     9061 aattcgatgc agttttagta ttaaaagaag atatgacaat cggtttttct tttccatgga
     9121 tcgaaaacag tggaacaaaa gcagttgatg gaaacaaaga aacatcaaga aaaaataatg
     9181 aaaacgaagt agagtaaatg aaaacaatga aatagcggaa tatttattcc gaaaagtgag
     9241 aatataaata ccaataaaac taaaaaatat cagtgaatca agcaaaaacg ttggggtagg
     9301 ctgattttat atagatagca aggagttttt ttatttgaaa aaagtgcatt catttttaaa
     9361 ttatcttatg ggagcaatca cgattgtggc tgtgtaccaa gcatttctaa agtatcctga
     9421 ttacgggacg aagttgttgt cattgacgca caatcaaaca gcatattcac caacgttgat
     9481 tttatcgact ttgactgcag gagctaaaag cgctacttat ttttttcata aaacgacaag
     9541 catgctaaag cgaaagaaac agcgaaaaca attgttggtg gtggtagcgg ctatgatcat
     9601 tatttatttt ctaccagtta ttacacaagt atttcatcaa tattttggtt aagaatacga
     9661 gagtttgtga caaaagtcat gtcacaaact tttttctttg tcattttttc gtaaga
//
LOCUS       DACCYH010000102         9446 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.107, whole genome shotgun sequence.
ACCESSION   DACCYH010000102 DACCYH010000000
VERSION     DACCYH010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.107"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            41..1015
                     /locus_tag="IVY85_002496"
     CDS             41..1015
                     /locus_tag="IVY85_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8045466.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            997..1458
                     /locus_tag="IVY85_002497"
     CDS             997..1458
                     /locus_tag="IVY85_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8045467.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1508..1972
                     /locus_tag="IVY85_002498"
     CDS             1508..1972
                     /locus_tag="IVY85_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8045468.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            1986..2735
                     /locus_tag="IVY85_002499"
     CDS             1986..2735
                     /locus_tag="IVY85_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8045469.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2735..3565
                     /locus_tag="IVY85_002500"
     CDS             2735..3565
                     /locus_tag="IVY85_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8045470.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3565..4212
                     /locus_tag="IVY85_002501"
     CDS             3565..4212
                     /locus_tag="IVY85_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8045471.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4310..5086)
                     /locus_tag="IVY85_002502"
     CDS             complement(4310..5086)
                     /locus_tag="IVY85_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8045472.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5230..6189
                     /locus_tag="IVY85_002503"
     CDS             5230..6189
                     /locus_tag="IVY85_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8045473.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            6306..6614
                     /locus_tag="IVY85_002504"
     CDS             6306..6614
                     /locus_tag="IVY85_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP8045474.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            6641..6940
                     /locus_tag="IVY85_002505"
     CDS             6641..6940
                     /locus_tag="IVY85_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAP8045475.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            7114..7665
                     /locus_tag="IVY85_002506"
     CDS             7114..7665
                     /locus_tag="IVY85_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAP8045476.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            7811..8104
                     /locus_tag="IVY85_002507"
     CDS             7811..8104
                     /locus_tag="IVY85_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045477.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            8097..8393
                     /locus_tag="IVY85_002508"
     CDS             8097..8393
                     /locus_tag="IVY85_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045478.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            8464..>9446
                     /locus_tag="IVY85_002509"
     CDS             8464..>9446
                     /locus_tag="IVY85_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP8045479.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMT"
BASE COUNT         3330 a         1364 c         1779 g         2973 t
ORIGIN      
        1 atatgacttt ataatactta ttagagtgag ggataaagaa atgagacaac tacacaaaga
       61 acagattgaa acagcaatgg cagcgttaaa ggaaaagaca attgatcatg tatattttac
      121 agcatgtggg ggttcacaag ccgttttaat ggctggtcaa tatctatttg ataaagaatt
      181 aactgtacct tgtcatgttt atactgctaa cgaatttaac tatgctatac caaaaggatt
      241 atctgaaaat tctttagtta tcacttgttc ccattctggc acaacaccag aaactgtaga
      301 agcaacaaaa aaagcaagag aatttggagc aatgactatt tgtttatcta atgaagaagg
      361 atctcctcta tgggaagcag ctgaatatcc tgttcactat gattggggta aagatgttga
      421 tgctagtgat ttaaataaag gaattcttta tggtttatta tttaatattt tacttgtttt
      481 agatggctct aaaaaatggg aaacttgctt gaatgaattt gataagttaa atgatcttgt
      541 tattgctgaa aagagcaaat ttgaaataac tgctaaagaa tggggtaaaa accaaaaacg
      601 agataaaatt atttacacaa ttggtagcgg tatcaattac ggagaaattt actccacagc
      661 agcttgctgg ttcatggaaa tgcaatggat taattctaat gctattcatt ctggggaatt
      721 tttccatggt ccttttgaaa tcactgatta tgatgtacca ttcatcttag tgaaaagtat
      781 tggagataca agatttttag atcaacgggt agaagacttc actaaaaaat tcagtaataa
      841 attaatggtt ttagaccaag ctgattttga cttagaatct atagatagtg tagcgaaaga
      901 atatattgca gctgtactta gtggcgttgt tattcgccaa atggtagaag cgattgcttt
      961 tgaaagaggc cattccttag atgttagacg ctatatgtgg caaatgaaat attaattaat
     1021 aggaggttgc aaaagatggc aaatcaatta tttcacattg tgattacaac acattctacc
     1081 ctttgtgagg gttatttaaa ggcaacaaaa ttaattttaa tgaatgacgc cacaggtatt
     1141 agtgctgttc catttaaaga aaatactagt ccagatgatt ttgaggagag tatgaaagaa
     1201 ataattagtc agcatcattt gcaacctcta attattttaa cggatttaat tggaggaaca
     1261 cctaataatg tttctattaa acagttgaac aacaatagaa ttcaaattat tgctgggatt
     1321 aatttacctc ttttactgga gttattaatg gcccagtcag ctggtaaatc tatagaagaa
     1381 atagatttta aggaattact gaataactct caaaaaagta tggttcatat taattcgctt
     1441 ttaaaagatg tcagttaagg tggcaatttc tattctataa attttaaaaa ttgaaggagg
     1501 aaatagaatg attaaattag tgcgtgtaga ccacagatta cttcatggac aagtaatatt
     1561 ttcttggact aaacagtatg atattaacca tattattgtg gctgataaca aagttccagg
     1621 agatccaatg tctgtgatgg ctttgacaat tgctaaacct gctgattgca aattagacat
     1681 tgttaaaatt agtgatgtta aagggattat ggaaacatcc ccaaatgata attacatgat
     1741 tttaattaaa ggacctaagg aagcttttga attagttcaa aatttaccag aaattaaaga
     1801 aattaatttt ggcggtgtgt ctaagaaaag cgagtccaaa caatatggaa aggcagtctt
     1861 tttaactcct gaagaattag aagccacaaa aaaaattctt gatcaagaag tgaaaatatt
     1921 tgtgcaacaa gtcccatctt caagtattga aaatgtagat tttagtaagt aaggggggaa
     1981 aataaatgtt attaaaagcc attttattag gtttagttgg tgtggtagct gttattgact
     2041 ccaggttaat tggtcgacaa aatattggta gaccactaat tttgagtaca cttgccggac
     2101 ttgtgctagg tgacttgcat acagggattg ttcttggggc atctttagaa ttaatttcta
     2161 tgggattcgt ctcaattggg gcggctggac ctcctaatat gcaacttggt agtattattg
     2221 ccactgcttt tgcaatttta actaatagta gtacagaaac agccttaaca atagctatcc
     2281 cagtggctgt agctggtgaa tttatgagta ttatcatgcg catgataatt gctcaatttg
     2341 gacatgctgc agataaagcc attgaaaata ataaatttag aaaagctcaa attattcata
     2401 tttattggtc atttattttt aatgcttttg tttactttat tccaatcttt ttaacagtat
     2461 attttggtgc tgacgttgtg gcagatttag tagataaaat tccagaaatg attacagacg
     2521 ccttgactgt ctctggaaat atgctttccg cattagggtt tgcaatgcta ttaagcacaa
     2581 tgttgagtaa aaaattatat ccttacttta tttttggatt ctttattgtt gcctattcag
     2641 gtctttcact aattggcgtg acaatttttg cagctctgat tgcctttgta atggatcaag
     2701 ttaaatatgg aaaaagagaa gaggcgcatg gttaatggaa aatagtacag aaaaaaaacg
     2761 tttaatcaca aaaaaagatc ttaataagat gtgtcttcgt tcatggacaa tcatgtgttc
     2821 gtggaattat gaaagacaaa tgcatatggg atttatgtat gggatggcgc caattttaaa
     2881 taaagtttat aaaaatgatg aagagaaaaa aagagaagct tatactcgtc atatggcatt
     2941 ttttaactgt acgcctcaaa tgacttcatt tattatggga ctttgtgctt ctatggaaga
     3001 acaaaatgct caagcagaag aaggaaactt taatactgaa tctatctcca tgattaaaac
     3061 cagtttaatg ggtccttttg ctggaattgg tgattcattt ttccaaggta ctttaagaat
     3121 tattacattt gggattggat tgtcttttgc taagcaaggt agtattttag gaccgatttt
     3181 agctgttggc ctttttgcta taccttctat tttagctgtt ttttacggcg gtaaattagg
     3241 atatgagtct ggcaataaat accttactaa attgtatcaa gaaggtctta tggatcgagt
     3301 aatgcaattt gcttctatca ttggtttggc tgtagtaggt ggaatggtgg ctagcatggt
     3361 gccaattgtc acccctttaa aattcactac agggggaaca gaacttgttt tacaagactt
     3421 acttgattcc attattcctg gattattacc acttgtatgt actttgggaa tatataaatt
     3481 gattaagaaa aatgttagta caaatatgct cttattaggt tgtattttgg cagggtttgt
     3541 tttaagtata ataggtatct tataatgatt aaggctctaa tttttgatat ggacggagtt
     3601 ttaattgata gcgagattat gtatcaaaag tgggtattta attttttaaa acaagaaaat
     3661 ttaagctatc ctttgtattc ttatcaaaaa aagattggaa ctacagcaag tatttttgat
     3721 gatatagaaa agtacaatga gggatgtaac agtttagaat tgaagggaaa atttactaaa
     3781 tattggaaat cgcaaaagat tgattacttg gatatttttc ctaatatact aattgaacag
     3841 cttgaatggt taaaaaagaa aaaaataaaa atggcaattg cttcttccag tccaatggat
     3901 actataaatg aagttgttac cacttgtaaa atagagtcat attttgattg tttaattagc
     3961 ggaagagatt tgccagaaag taaacctaat cctactatat tccttaaagc ttcagaacag
     4021 ctcagaattc cagttgaaga atgtctggtg atagaagatt cttataatgg tataaaagct
     4081 ggtaaaagag caaatatgaa ggttcttgca attaaagata aacgtttttc tcaagatgta
     4141 agtctagcag atgatgttat ttacgatatt aaatatctgc gacaagaagt acaggtgaaa
     4201 tttggaatat aaataagttt tttgatgtat ttagagaata ttagtaaaaa taaaaatcat
     4261 gcccaaatct aatcaaaatt gattgctttg ggtatgattt cttttaatat caatctatta
     4321 ctttaggatc tgaatcattt ttatgcaccg tatctaaagt gatattttct gtgacttcaa
     4381 taacataact aattctatca caacgaactg tggattgaga ataatgaaat ggttgattat
     4441 ttttatcttt gccaaccttt tctaaaataa atagcggatc tcctactata cattgcaata
     4501 agtcggaaag ctcaggtgtt gcagataaac cgtttatttc cataatagag ctcgtagctt
     4561 tgacatcata gtcatcttct agaagtttat ataatgaagt atattggcta cctttttcaa
     4621 ataaagttgg aaatttttct tctgatgcga aaaaattatc aattcccatc ggggcaccgt
     4681 cttcccacat taaacgttta atattgtaaa ctctatcagt tacattgatt tgtaatttat
     4741 tagctaaaaa ctcatcagcc tttataattt tagaatgtaa aacagtatat ctaattttat
     4801 gtcctcgggt agaaagagaa tcgctaaatc catgaactcc agctaatttt aaactttctt
     4861 ttttttgagt tacaaaggtc cctttccccc ttaatcgttc aactgttcca ctttctgcta
     4921 aaagtttaaa agcttttcga atggtaatct tacttactcc ataaatttta cataattctt
     4981 cttctgtagg taattgctct cctggaagat attttttttc acgaatatcg ttacgtatag
     5041 aagcttctac ttgtaaatat aatggtctat tatcttgaaa attcatagta cactccctaa
     5101 aaagttatac ttcattatat cacaatttat taaaacgtcg attgtaaaat taagctagac
     5161 aactaaaaag atcatttgtg ctacactcaa aatagttccg accaaagaat taataaggag
     5221 tgagtacaaa tgacttacac ccatcttaca acagacgaac tagtaatcat agagtcttat
     5281 ttcaatatcg agaaacctgt cgctcttgtc gcacaaagtt taaatcgctc tagacaaacc
     5341 atttataatg tttattcgtt tctcaaacaa ggaaaaaaag tgctagatta ttaccgtcaa
     5401 tacaaacaaa ataaatctcg ttgtggacga cgttcaaccg ttttatctga tgaacaatca
     5461 acatatgttc aagaaaaggt agctcaagga tggtcacccg atgtgattat cggccgtggc
     5521 gagtttccaa ttgattgttc tgttcgtaca ctctatcgga tgtttaaaaa aggccttttt
     5581 gacacacagt cgttacctat gaaggggaaa cgtaaaccga ataatcatca agaaaagcgt
     5641 ggaaaacagg cctttcgcag aacgattcat gaacggaaca gtctctatcc acacctgact
     5701 aaagaatttg gtcatctcga agtcgacacc attgtgggag ttaaacacaa gagcgctgtt
     5761 attacattgg ttgagtgttt atccaaggtt attattactt tgaaaccgac aggcagacga
     5821 gccgaagata tcgaaaacag gttaaatgat tggttcaaat ccatgcctaa acacttgttt
     5881 aaatccatga catttgattg tggaaaagaa ttttccaact ggcgatctat aagtaactcg
     5941 aacgatattg ccatcttttt cgcagatcca ggaacacctt cgcaaagagg gctaaatgag
     6001 cactcaaacg gcttgcttag acgagatggc ttacctaaaa caatggactt tagggacacg
     6061 gatgaaacct ttattcaatc cgttgcctcg aaaagaaacc atattccaag aaaatcatta
     6121 aattatcgaa caccattgga agtatttttg agctgcatag ataaagacat attgtctagc
     6181 ttaatttgac aaatcagata gtataaaatg aatcaaattg aatatcaaat atatattata
     6241 tccatgaagc ccaatcttct ttaatagaag attgggctat ttttatggca aaggagggaa
     6301 aagaaatgtt tcaaaataaa ggacaacgtt atgtcacaaa gggagtaatg gacagtttgc
     6361 cggtggaatt acaagcttta tgttggaatc taatcgatca aaacgttcaa aagcaatttc
     6421 atttagacta tcttcaaatc tttgaatttt ctacggaaaa aggaaatcaa aagttggttc
     6481 atcgtcaaga agaaccagaa gcaagaaaag agtatctgat ttcacctaag cttcgtttga
     6541 aatcagtgtc tcaaaaaata tgggtcattg attcgggtga gtatcaaacg atgttattac
     6601 cagaagaata ctgatatcta aaagaaagga acgaaaggac ttgttagaaa cggctatcga
     6661 agtactggaa aagtgtgcac agctagtcac tactcctgaa gagtggggct atgaatccgt
     6721 tacgatggaa aaagaggaaa ttgaaatggg tatgcttcct gaagatgtta ctctaccacg
     6781 tttagtgatg acacatcttt atatttattg cgcaccagaa gatggaaaag actatgtagt
     6841 ttatttcatt acagatatta ccagccagcg agaatttgtt cgtgggttac tggtagaagg
     6901 acgattggtt tggagtcaga ttggaggaac gaatgaataa gatcgattat ttgagaatga
     6961 ttgcgaaatg tttagaagat atccttacag cgattcatca aattctgaag aaaaaaggag
     7021 aaagaatatg aaatattttc aactagtatt gacacttata acggcaatgg tgattgaatt
     7081 gcttgaacaa aaattagcat aggagaaaaa gagatgattg aaaaagaatt agtgaccaca
     7141 caaacagaag tttggcgaga tcctcatcat acccatcgct atgttttcaa acgacaatgg
     7201 gcgatcaaag gaaaagaagc ggaagataaa ttagcggtag tcattactat tcgtccaaca
     7261 gatacagaac catttacgaa tgatttgagt atgttattaa tcgaaaagaa tatccgacaa
     7321 ttaggattta ctggttttat tgctgtgaat cttttttcgt atacaaaagc gaaaagtcct
     7381 cgggtatttc ctcgtggaaa tgatgaacaa tctttggaaa tattaacaac tgttttcaca
     7441 gaaaagaaaa tcaacaccat tatttttgct tgtggttcga ttacagcgac gagtcaagtg
     7501 gcttatgaac aagcgaagac catttacgat caattgagtg cgaaacaaaa aaagatgacc
     7561 aaagtattga taaatgcaga aggtaagtta tcccacccac taagtattca tgttcgtaat
     7621 gaatggcaat tggcagatag tcaattatta tttcaagaaa aataacattt tattttctta
     7681 tgttacttat aagtagtttt acatgctttt tgactttaaa aatacatgtg atgatacatg
     7741 ttatatgaat tatcaatgta cccagtaata tgctattttc attatgtaaa aaataacagg
     7801 gggtgtgtag atggagattg atgaaacttg tttgagtaac tcttataaaa gtatgaatga
     7861 cttttttgag gaaatggaac tggattcaga attgctctat caattttatc ttgcttttcg
     7921 agggcaacag atttcttttc caatgaaaat gtacgatcgt gagttagtta gaaaaagaat
     7981 cgaaaacatg atagaacagg aaaaaactgt agatatccgt caattgacag aagtttatgg
     8041 tttctctgca agatggattc aaaaagtaat taaacaaaaa gaaaggagac gagtacatgg
     8101 ctaagctgat tgtattaggt ggtttaattt acggtggata ttggttatat aaatatatgc
     8161 ttgcgcatcc gaatatagat aaaggaaaag taattaagac agttggggta atcatagttg
     8221 gactcatagc tctaactaag aaaaataata ctaatagccc aaaaataatg agaacttatc
     8281 gctgtagagg atgtggaagg gaaatttcta ctagttttaa tccatctgaa agccctcaag
     8341 gaagagatcg ttgtcatgct ggctatcgac attcttggga aaggattaac taaatgaaca
     8401 ccattgagat taattgactg ataaaataag tacgcgaggg agaaattgaa agggggaaat
     8461 aaaatgaaaa aatggttgtt attactaatt ggaatgggga ttttattcac tggttgtaca
     8521 tctgagaata cgaggactaa agagactgta gaaactgata caacaattga ggctaaaaaa
     8581 agtaaaacgc aatcaactga acaaactaca acagaaatga aacaatctgt ggaagcaagt
     8641 acaagtgaaa gttcaatcat tgaatcttct tctgaagagg ttaaaaatac tttgtgggat
     8701 agtgagaaat ctactcaact agcaattttt gtcccacaat ggggaaagac attaggtcaa
     8761 gaatataaaa gctacaatcc tcagaacaat gtaagtctgt atggtacgcc tttaccaagt
     8821 gcagttattg atggaaattg gaaaatggca atcaatgaag caccagttac cgtacaatgg
     8881 tcagaagatg gaactggtca tgcagatttc aatttagtag cagtttattc tgatgtagaa
     8941 actggagaat atttagggca gcatgtatat ttctttggct ttcaaaatgg acaacctaaa
     9001 gtttatttaa cgcaacaaaa tcaaggcaat gataaaaact acatgtactt caatgaaact
     9061 cagaaccaac agttaaaaca aggtttcatt gatattgtga atggacaaac tcctcaaact
     9121 ccagtagtag aagaatctac tcaacaagca acaactacaa cgacacaatc tacaagtagc
     9181 tcagcaattt caagttggga agctgctaaa gaaactgtac taaataataa agctttatgg
     9241 ttgtatgatt ctcctgataa aaatgcatca aatactaatt taattagcac aaatgaatat
     9301 atgaaagatc taatgcaaga tgaaaatgga agctactatt caattaatat tgtaacggat
     9361 gataatggaa atacaacaat ggctggtgga aaacaattcc gtgtttatac agatggaaga
     9421 atttctcaac gtattggaat gactaa
//
LOCUS       DACCYH010000103         9443 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.108, whole genome shotgun sequence.
ACCESSION   DACCYH010000103 DACCYH010000000
VERSION     DACCYH010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.108"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(130..777)
                     /locus_tag="IVY85_002510"
     CDS             complement(130..777)
                     /locus_tag="IVY85_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP8045480.1"
                     /translation="MEQTIKPYQYFFIKREVDQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(1056..1559)
                     /locus_tag="IVY85_002511"
     CDS             complement(1056..1559)
                     /locus_tag="IVY85_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8045481.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(1682..2446)
                     /locus_tag="IVY85_002512"
     CDS             complement(1682..2446)
                     /locus_tag="IVY85_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP8045482.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     NAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            2553..2828
                     /locus_tag="IVY85_002513"
     CDS             2553..2828
                     /locus_tag="IVY85_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP8045483.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            3505..4455
                     /gene="yidC"
                     /locus_tag="IVY85_002514"
     CDS             3505..4455
                     /gene="yidC"
                     /locus_tag="IVY85_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8045484.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(4634..5608)
                     /gene="lacD"
                     /locus_tag="IVY85_002515"
     CDS             complement(4634..5608)
                     /gene="lacD"
                     /locus_tag="IVY85_002515"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8045485.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKIPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATPVYVDR"
     gene            complement(5605..6063)
                     /locus_tag="IVY85_002516"
     CDS             complement(5605..6063)
                     /locus_tag="IVY85_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8045486.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIALDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIATLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            complement(6060..7523)
                     /locus_tag="IVY85_002517"
     CDS             complement(6060..7523)
                     /locus_tag="IVY85_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8045487.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGIKIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAMSGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMVKAVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLAQGN
                     TTFMAGVSAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEKGRAGDLS"
     gene            complement(7505..8455)
                     /gene="pfkB"
                     /locus_tag="IVY85_002518"
     CDS             complement(7505..8455)
                     /gene="pfkB"
                     /locus_tag="IVY85_002518"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP8045488.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPKIPQTAVHNFLNQIRALQAEDYLCVSGSLPRGLSPKVLIEISRICQEKGI
                     HLIIDSSDQEAMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVELGAENV
                     LLSLGEKGAILFTKDRVFRGNSPKGKVVNTACAGDTMLGAFLAGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFETWENIN"
     gene            8598..9347
                     /locus_tag="IVY85_002519"
     CDS             8598..9347
                     /locus_tag="IVY85_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8045489.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARILLDAYVLRMGEN"
BASE COUNT         2806 a         1998 c         1640 g         2999 t
ORIGIN      
        1 aaagaaaggg gctatcccca gactttagta ggcgtcatta gctatctttc gttagcattt
       61 aaaggcgaac accatcacct attcacttcc attaaaagat agcatatttt tctgtaaaaa
      121 acaataaaat tatcgttctt tctttacacg ttcagcaaat tcatagaaag gagccaattt
      181 cgttaactgt tgattacatt ggtcactatc atgacaaata taattcccct ttttcgtata
      241 cgtgccatca gaacctgctt tcgttgtaga aagaaaaagc gacacattcg atgttttctg
      301 acagattgca cagattccct tatttatcgt tggtgataaa gtaccgctaa tcccttttaa
      361 tttttcatct tcgtaataga ccatgaattt tttctgcgta cctggatcat tccagccgat
      421 gtacgtatgt tcttgcaagt cgatttcatt ccaagctggt tgtttgagtt ttttcacttt
      481 tcggaataat ttctcgattt gtggtttgct aggctgcata aacggctgga cgatttgctt
      541 cagctcctcc aatgctcttt ctgcttgcgc ttttgtcagt tttacgtcta aaactgtcgt
      601 taaaaatgta tcgatttctg gcagttcgtg ttctaatatg tctcgaatct tttcagctgc
      661 tagtgcctga actgtttgta ccgtttttgg atcattgaca gatgagtacg catttaataa
      721 ctgatctact tctcttttta taaaaaaata ttggtatggt tttattgttt gttccatgat
      781 tttaaagtcc tttcttctaa ccaaagtgca tactttgtag aaaaaagtga tcttctgcaa
      841 agtatgtgtt acctgaagat cccggactct tttaaataag atacgttttg catcttatca
      901 tcaccccctt taatgttcat tcttctatgt acatgattca ttcgataaat aaaagtaaac
      961 cgtatcttga acagatagtc aactgcaaac agaaaaaacc gagaaaatca gccagatttt
     1021 ctcggaatat agtctattat tattggtttt acctattaaa tcgaggattc tttcccaaag
     1081 taacgttgcc atctcttacg catacttgca gacatgggct gagccgcttc tttgactgcg
     1141 cagtatttgt ccacgaatgt cacaatatat ccttctttat acttaggcgg tgcgatcgtt
     1201 gcaccccaca tgtgcttcaa aatgatatct cgctcaagat ccgacaaatc ggtgattttt
     1261 tctgcattct ttacagcaat tcttggatgg atatacgcat gtgtcccctc gtcaaatttc
     1321 gtcgtacgcc aatcatagta gaacagatca tgcaaaagtc cagcacgcgc tgttgcttta
     1381 gcattgccac cccatttttt tgcgagcaga taactatagt aagaaacact gatggaatgc
     1441 tccaaacgag tggaatgaac atgctgtgtg taattcgcta attttttcac tgcttctgtt
     1501 tctaaaagat cttgtacata agacatatat tcttcgtctt cacgccattt ttctgtcata
     1561 attatcgatc tgccaccttt ttgaatttca ttttcttctg aaattgatct aagtatacca
     1621 ccagtgttcg gattattcta gtcgctctag ataaaatagc taaagcttaa tagttcctta
     1681 atcataaaga aaaatgataa agaacgatcc ctgctgcgac tccgacattc aatgattccg
     1741 catttccttt cattgggata tatacgttct gatcagtcaa agacaagatt tcctgactta
     1801 ctccctgacc ttcgtttccc atgattatag cgaaatcctc tgttttttct actttgtcta
     1861 atgcatgtgc ttcttcattt aattctgtac cgtacaccgt aaaccctttg cctttcatta
     1921 cagggattac atctgccaaa tctttttgta acacaggcaa atgataattg cttccctgca
     1981 tgcttcgcag cacctttgta ctataaatat ctgccgttcc tttacctaat aaaacaccag
     2041 ccataccaaa cgcatctgcc gtacggatca tcgttcccac atttccagga tcctgtacat
     2101 tatctagtaa aagccagcct cctgaaaaat ctggcgtatc ttcagatgtt aacgtcatca
     2161 ctgctaagat cccttgcggt gtaggtagat ctgacatagc ggtcatgacc tcatctgaaa
     2221 ctagaaaagt agaaatctct ttgtttacag ccagccagtc tccccattct ttcatacctc
     2281 tctgtgtcac aaaaagttct tttatttctg cattagacga cgcagcttct tcgatcagat
     2341 ggaacccttc caaaagatat tctgcctttt cttctctgta tttcttttta tgtagttttt
     2401 ttaattcttt gattcttgga ttttttgctg attggatctc tttcacattc ctcacctcaa
     2461 ctgattatat catggaatag gtgcttgagc ttgtttcatc ctaaaaaaat cggtatcatt
     2521 gatttataaa acaggaaaga aaggtgacga acatgcgcaa agttaaaatg aatgtccaag
     2581 gacgtgtcca gggcgtggga tttcgctata tgaccaagat ggttgctgat cagttaggcg
     2641 tgaccggaac ggtaaaaaac gaagacgacg gctcagtcac aatcgaagca atcggaaatg
     2701 acgacatcat ccaaaaattt atcgaagaag tcaagaagtc tccgagccca agtggacgag
     2761 tgcagtatgt ggatgtccaa gaagatccga tgattgagga acgaaagaaa tttgatgtag
     2821 tgggataagg ttgtgaaaag gtcgcttagt tttgagtatt aaggaaaatt ttgtgaaaat
     2881 ggctttccac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     2941 tcgataaggt tgtttttgaa gcgctttgcc tcgagtatta aggagaaaca ttttcaaaat
     3001 agcttctcat attttgaaat gtaatgactt aataccgaga ggcagctgat aaaacaccgt
     3061 caataaggtt gtgaaaaagc tggtctgcat caagcattaa ggactggttg tgataaatag
     3121 ctctccatat ttttcaacag gctggactta atgccgtaga tgcaagcttt tgaacaccgt
     3181 cgataaggtt gtttttgagg cgctttgcct caagtattaa agacaggtag taaaaaagct
     3241 gatctataac ttgcaatgaa tttgcttaac ccatattttc cagacagaac aaaaaagttt
     3301 ttagtttttt aaataagaaa gaaacacttg gaatcactct tttctttcta agtatttctt
     3361 tcttgatttt ttgtcttttt tcaaaatttt tcgctacgaa aaagaacgaa tatttatatg
     3421 aaatttctat gaagttgatt gaattccatg cgctttttta gtatagttaa gtttgtgtaa
     3481 aaattataga agaggaagta ttctatgaat aaatggaaaa actggttatt gggatccgga
     3541 cttgtcagta tcctgctctt tttatctggc tgtgtgcgga tggattcaaa cggaaaccct
     3601 gacacttcag gaatcgttta tcgagtactc gtccatccta tgggccaagc gattacatat
     3661 ctagttgaga atttcaattg gtcttacggc tgggcagtta ttgcaatgac tgtgatcgtt
     3721 cgtatcatca tcttgccact aggtatcagt caatcgaaaa aaacaatgat ccaatctgaa
     3781 aaaatgcaag cgttaaaacc acaagttgaa gcagcacagc aaaagctaaa agctgcaaca
     3841 acacgtgaag aacaaatggc agcacaagca gaaatgcagc aagtatatcg cgaaaatgga
     3901 ttgagtatga ccggcggaat cggctgtctg cctctattga tccaaatgcc gatcttctct
     3961 gccttgtatt ttacggcacg ctacacagaa ggtatccggg aatcatcttt ttacggcatc
     4021 gatctaggct caccaagtat ggtgcttgtg gccatcgcag gggtcgctta tctgcttcaa
     4081 ggatatattt caacaatcgg tattcctgaa gaacagaaaa agacgatgcg tacgatgctg
     4141 atcgtctctc ctgctatgat cgtcttcatg tcaatcagtg cgccagccgg tgttacactt
     4201 tactgggtag tcggtggtgt gttcagctgt ctgcaaacat tcatcacaaa cgttatgatg
     4261 aaaccacgtt tgaaagcaca agtggcagaa gagttgaaac aaaacccgcc aaaacaagtc
     4321 gtcactccaa gaaaagacgt gacagaatca gcaaagccag ctaacaacaa acaagcaaca
     4381 aaacaaatca aacaaccaaa aaacagtaca aacaacaaag gccgcaacgc aggaaagcag
     4441 cagaagcgga agtagatagt aaataagagt gtaataaaaa ttcattaaca gttttagcaa
     4501 ttaaaaatat cgaccaagat ttttgaaaaa gaaatctggt cgatattttt atttaagtga
     4561 atgtttaatt tgaatcatcg attgatcgct atctaattgc agcagcatct ttttatgaag
     4621 aaacaacttt ttcttatcta tcaacgtaga caggtgttgc tgtttcttct aatagtgtac
     4681 ttaattcttt aatattttgt tttccttctt ctaacatcca actactcgct ttttcttcgc
     4741 cttcggtaac aaaaggtgcc actcccttcg cccatgttgc tcgcccacat aagacaccat
     4801 tgaatgtcga acctgattgt tttgcaaaac gtaatgtatc ttgaaatagc gtagcattga
     4861 ctcccgcact taaaaaaata aacggttgtt cagtcacttt cgattgttca tagaaatacg
     4921 cggatgcctc tgcttgacta taacaagtct cttgcccata tccttccaca aatctcatat
     4981 tcactggcac ttctactttc aatacatcca cttgataacg ttcttttgaa aattctcgca
     5041 tggcttcaat aactttatgt ggttttactt ttgcataggc aagagaagac ggatcgccaa
     5101 tagttgcatc ataagaaaga atctctaaaa agaaaggtat gccttctgct tcgcattctg
     5161 aaccaatccg ttctataact atttgttttt gcacattaat ctctgctggt tcatcaacgt
     5221 catagtacaa taaaaattta caagcatccg caccagcttc ttttaacctt ttcaccgacc
     5281 aaatattcaa taaatcaggt aatcttcccg gtattttcgt atcatagccg gttttttcat
     5341 aagctagcag taagccacat tctttctctc gaagcgctgc tgcaggcagg ccgtattctg
     5401 gatcaagtaa aatagctgag gcatagggag tcaggtattg agaaaccatc tgtttaaatt
     5461 ggatgatgtc ttctgatgta gcttctttat attggttgat cattttcttc aaagcaccac
     5521 gctggtcgat tgctaatgcg ctaataattc cagcttcatc cgtcaatcgc ttcaaacgtt
     5581 cctgtttttc tatactcatc ttcttcatag atcttcacct atttctttag ttaatattgt
     5641 ggctatttct gtttttgatt ttgcttttct caactgttct gtaacctctt ttcgcatcaa
     5701 tgatcgtgca acgagagaca ataatttcaa atgagtcgtt cctgcttctg aatctggaat
     5761 aaataacgca aaaataaaat caactgactg tctatctaaa ctgttccatt caattccttt
     5821 gttcagtgtg gcgataatca tactaggttc gttgattgtt tgagacttcg catgagggat
     5881 agcaaaacct gacattattc ctgtagtccc ttctttttct ctttctagaa gtttttgata
     5941 tacttctttt tcgtcaagag cgatccctaa acagaatgtt tgctttgcta aaaacttaaa
     6001 tgcttcttct tgactatcca atggctgttg taaaaaaatg ttctcttcat gaatgattgt
     6061 catgataagt ctcctgctct tcctttttcg ataactgtac gatttgctgc tttattatcc
     6121 atcttcttgt ctttatcagt tactcttttc ttttggatta atcctagtat cactccacca
     6181 acgatcgaac caataagaat cgctgtaacc cattggattt ttcccgtcac tacaggtaac
     6241 actaaaaaac caccatgtgg tgctggaact tggacgccaa acaaataagt taagacagca
     6301 gcaattgatg agcctaacat catcgtgggt aacactcgga tcgggtcttt cgcagcaaac
     6361 ggaatagctc cttccgtaat atgagttgat cctaaaataa aattaaccaa acctgcatta
     6421 cgatcattgg tatcaaaata tttaccaaac aataaagtag caaaaccagt aatcaacggt
     6481 ggtgcaatac aagctgctga cacaccagcc ataaaagtcg tgtttccttg tgccaaaaga
     6541 gctgttcctg ttacatatgc cgccttatta accggtcctc ccatatcaaa cgcacacata
     6601 cagccaacaa tcacgccaag aacaagtgga ttcgaatgtt caaatccagc taaaaagctc
     6661 atcattcctt catttatagc cttcattgga cctgccagca aagacatcac gatgccaata
     6721 aatgctacac caataactgg atataagaag attgccttca agccatctag ttgtttaggt
     6781 aaaattgtca gtagcttttg caaaaataag ataaaataac ctgctaagaa cccagctaaa
     6841 atacctccta aaaatccagt accgccattc atggctacga ttccaccaac aaatccaacg
     6901 actaaccctg gacgtttcgc gatcccttct gcaatataag ctgctaaaac tggaaccatc
     6961 attcccattg ctgcatcgcc aactgctttt accattgccg ctgtagcatt gtattgtgca
     7021 tgatctggat caaaagaata gattccccaa acggcaaatg aaatagcaat caatactccg
     7081 ccagctacca cgaaaggcaa catatgagag acaccgttca ttaaattttt atagatgcta
     7141 cgaccaaaat tagcaccact catcgcttct ggttctaaag tatcagcagc ttcattatct
     7201 gctaagacag taccttttcc tgccaatgct tcgtcaatca gccgttgtgc atccttgatt
     7261 cctgtactga cagaaacatc aatgactcgt tttcccgcaa atctttctga atggacatct
     7321 ttatctgcgg caataatgac agcatctgcc tgtttgattt cttcagccgt taattcattt
     7381 tcgatcccaa tttgtccatg ggtctccact ttgatcttga tgcctttttt cctagctgct
     7441 tgttctaatg cctcttgcgc catgtaagta tgcgcaattc ctgtcggaca accggttgct
     7501 gcaattagtt gatattttcc catgtttcaa acctctcttc cttgattttg atttgtttac
     7561 ttaattcctc cacatccaaa aaatcagtca gtcctttacg aaaagcggtt gagctgcctg
     7621 ctgctatgct tttacgtaac gtttcatcaa gcggtcgtcg attcatatat cctgctaaaa
     7681 aagcgcctaa catggtatca cctgcacatg ctgtattgac aacttttcct tttggcgagt
     7741 ttcctcgaaa caccctgtct ttcgtaaaaa gaatcgctcc tttctcaccg agggataaca
     7801 aaacattttc tgcacctaac tctactagtc gttgtccata aacaaagtaa tcttcatctg
     7861 taaccatttt tcgtccgaac caagaagaaa gttcttcctc gttcggtttc agcaaaaacg
     7921 gtcgataggg taaacaatcc atggcttctt gatcactact atcaataatc aaatgaatcc
     7981 ccttttcctg acaaatccga ctgatttcta ttaaaacctt tggagacagc cctcttggta
     8041 aactcccaga aacacaaaga taatcttctg cttgtaatgc tcttatttga tttaaaaaat
     8101 tatggacagc tgtctgcggt attttaggtc cttgattcac taatttgtat tcttctcccg
     8161 tttgattgac ttgtgtaaat acattgatac gcgtcatacc aggcacttca ataaactctg
     8221 tttggatttg tttctgctgt aaatagtctt ctatatactt tcctgtaaac ccagcagaaa
     8281 aacctaatgc cgtacttggg aaatctagca tttttaatac tagtgacaca ttcacccctt
     8341 ttccgtttgc ctgaatgtca tcctcttttg tgcggtttac cataaaagga tgcatctcct
     8401 ctgtttcaat aaatagatca attgccaaat tcatcgtgca tgtgtagata gacataacca
     8461 acgacctcct tgactctatt atgaaatacg cgaaagagaa aatgttttct taatcgtccc
     8521 aacacaaaga aaacctttac atttataaac tcgtcgtgct attttttaac taacttttgg
     8581 tgaaagaagg aaagaatatg gatcacaaat taagtgaaac agaacagtac ctctggaact
     8641 ttatcgaaca ccacatccta gagatcccta actactccat cgtgaaacta agtgaacaag
     8701 caaatgtctc aacagccacc attgtgcgga caatgaaaaa gaaaggctat gaaggattta
     8761 cttcgtttaa gcaccatcta aaagaaggag aaaacaagaa catcaatttt tctttcttag
     8821 ataaagtaga caaaggtatc cgtcgagcca ttctaaaaaa tgaacaagaa gtggtacgta
     8881 cgatcaatat gttggaaatc ggaaatattg aggatgcaat acaaagaatc aaatttgctg
     8941 accgggtttt tgtctttgca cgaggatttt cagaaatgat tggtcaagag atgctcgtaa
     9001 aacttcaatt aacagggaaa aattgtcaaa tgcatactga tccagaaatc atcaaaagca
     9061 tcagtcggaa actaactaag aaagacagcg ttatttttgt ttctttgaac ggcgagacaa
     9121 aagaactcgt caccgccgca aagaattgct atgatgcaga gattggttcg attctagtta
     9181 cagccaatca ttcctctaca ctgaaagact attgcgagat caatctcgta ggtttcaaat
     9241 cagaaggttc ttatttccct gattatgaag tacggtcacg tttgcctctc cagattatag
     9301 cgagaatcct gcttgacgcc tatgtattgc gtatggggga gaattgaaaa gcagtttatt
     9361 atggttggtt acagtttaaa acatcacatt ttttattctt tatattgtat ggactcacgg
     9421 aaacttttta tgtataaagt tat
//
LOCUS       DACCYH010000104         9194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.109, whole genome shotgun sequence.
ACCESSION   DACCYH010000104 DACCYH010000000
VERSION     DACCYH010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.109"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            63..1277
                     /locus_tag="IVY85_002520"
                     /pseudo
     CDS             63..1277
                     /locus_tag="IVY85_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1456..1791)
                     /locus_tag="IVY85_002521"
     CDS             complement(1456..1791)
                     /locus_tag="IVY85_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8045490.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1775..2047)
                     /locus_tag="IVY85_002522"
     CDS             complement(1775..2047)
                     /locus_tag="IVY85_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP8045491.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2498..3301)
                     /locus_tag="IVY85_002523"
     CDS             complement(2498..3301)
                     /locus_tag="IVY85_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP8045492.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3279..4403)
                     /locus_tag="IVY85_002524"
     CDS             complement(3279..4403)
                     /locus_tag="IVY85_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8045493.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4419..5234)
                     /locus_tag="IVY85_002525"
     CDS             complement(4419..5234)
                     /locus_tag="IVY85_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8045494.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5224..6105)
                     /locus_tag="IVY85_002526"
     CDS             complement(5224..6105)
                     /locus_tag="IVY85_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8045495.1"
                     /translation="MSEMKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAP
                     TKEFVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNL
                     YKPLFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAG
                     ITAWKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVM
                     TIVQGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAV
                     FLGVIFKFVEKGVYYEN"
     gene            complement(6181..7470)
                     /locus_tag="IVY85_002527"
     CDS             complement(6181..7470)
                     /locus_tag="IVY85_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8045496.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7698..8342)
                     /locus_tag="IVY85_002528"
                     /pseudo
     CDS             complement(7698..8342)
                     /locus_tag="IVY85_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
     gene            complement(8819..>9194)
                     /locus_tag="IVY85_002529"
     CDS             complement(8819..>9194)
                     /locus_tag="IVY85_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8045497.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVWLSVRTAFFVG"
BASE COUNT         2829 a         1966 c         1466 g         2933 t
ORIGIN      
        1 caaaatcaat tctaccttag atgttcttat atcactctat cagtcctatt tgacaaagag
       61 ccaagaaaaa gacgttggtc gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa
      121 gttgtggttc tactgtggta gatggaaacg ggaaagcaat tatagtgaaa aatgggaaga
      181 aggaaacgat tgtccgtttt gaacaataca atcatatgcc tttggttacg cgcctaaaaa
      241 agcaacgcta tacctgtaaa aactgtcgta cccattggac ggctcaaagt tattttgtcc
      301 gactcagcca ttcaatcgca gatcatgttc gatataaaat tgcttcttta ctgactgaaa
      361 aagtatcttt gtcttttatt gcgaaaagtt gtcaggtatc tttgactacc gttactcgta
      421 cattgaaaga gtttaaaagc tatttaccaa agccttctaa gaagattctc cccagagtat
      481 tgatggttga tgaatttcgt tcgcacgctt ccatagaaga taaaatgagc tttatttgcg
      541 cagatggtga aacaggaaac ttaattgctg tcttgcctac gcgtaaatta cctcgattaa
      601 caagctattt cttaggctgc accaatccag aagaagtaga attcttagtc acagacatga
      661 acgccgctta cttccagctt accaaacgtg ttctgccaaa tgcgaaagta gtgattgatc
      721 ggtttcatat tgtcaaacac atgaatcagg cactcaatga gttgcgtatc cgtgaaatga
      781 atgagcttcg taaagcagga cagaaaagtc aggcagaaaa actgaaaaag aactggcgct
      841 ttctgctaaa aaatcgtgca agcatcaacc attatgagta caaaacatgg aaaagtttcc
      901 gagcaccaaa atacccattt ctcactgaag caatgatgat tgatcgattg cttgagtttt
      961 ctgcgcctct aaaggaggcg tatccctttt ttcatgaatt agtcgaagcc tttcgagaca
     1021 aagaccctga cttatttttc tccttattgg cagaacttcc cgaaacgtta gatgacggct
     1081 ttcgggagaa gcttcaaaac cttctgacct atgaagaagg tatcaccagc gcaatgatct
     1141 atccttattc caatggaaaa attgaagcga agaataccca cataaagaca atgaaacgag
     1201 tatcctacgg atttaaatca ttcgagaaca tgagaattag aatctttttg atcaatcaat
     1261 taatcaatgt aagataacaa aaaatctgaa ccagaatgtg gttcattctg acccggattt
     1321 gattttgcac aactcatcag tccttattga caaagagcca aaaatctgag ccagaatgag
     1381 gttcattctg acccagattt gattttgtac aacccatcag tccttattga caaagagccc
     1441 ttctttttag tattattata attcttccat gcgttcttcc agtcgctgta aatcgcttgg
     1501 acttaattcc ccaatttttt tccatttgac ctttttgttg tttttgggta attcgtttag
     1561 agttcctacg tcaatccaag attctttttt tagtcctgcc tgtttccaat cccttatttc
     1621 aacgtatttc agctttattt tatcggattt agtatgatat tttgaagtaa ttgacagaaa
     1681 cgaaattgtg tccagtccat tttttagaat atagacaggc cggcgtttac cgccctctgc
     1741 agctgtgaaa gcaacgtaga cggttgctat atcattagta ttcatcttca ttgaaccatt
     1801 cttcaatttt ttcgtcagtg tctagttttt ggacaggcaa tttttccgct aatctttgaa
     1861 tattatggat tcgtttactt ttttccgaca attcaataga aaatgggatt tttccttgct
     1921 caacaatttg cttataaaaa acggtgacgg ctgttttggg gctcatgcct agttcatcaa
     1981 taatgctttc tgcttcattt ttcaattttt cgtccataga aatttgtaac ttagccattt
     2041 tctccacctc tttctgacaa acacattata tcacggaaag agttcaaatg gaattaatac
     2101 aactactttt gaactccttg aaatgcagtg tatttatcta aatataagga aaggatatag
     2161 tgctaaatgg ccttgataga tagaaaagtt tcaaaatccg ccacagcgat aattgaagcg
     2221 aacactcaac gacacacaga acaggaaaag actcctaaaa gaaccaaatt aactaggacg
     2281 tgtctgaaaa ctccacaaag atgtggtatg ctctggtaaa aaacgaatgg cacaaccacg
     2341 aagaccgata tggttcttgg aaaactttct ccaccctttt tgtaattgga gggatagtgg
     2401 tttattgata gcgattttcc aagttcttca agtggaacca gacattgagc accacggcta
     2461 aaaagtgaat gaagtcttta tttttaatca ataattttta atgattacca gtaattggtt
     2521 ttttcatgcg ccctatttct atttgatgtg gagttaaata atgctgataa gtaaaaattt
     2581 ttccgtcaat taaactgcca ctagtgaatc ctgttctatt ggtaaaaaga gaatagtctg
     2641 ggtattcatc tacaccaaat gataaagatt cagccataaa ctgtttttta ccagcaaatt
     2701 cagcactgct accttgatgg atatgtccta caaaaatacc taaaatatca ctttcggcaa
     2761 tcaattcagc aaaaccttcc ggaacttcag ttacaataga tggatcttcc cacgacaatg
     2821 gatgatgaac caataaaatc gttccctttt cagcaggtgt acccaaccac tcttgtaatt
     2881 gaatcatctg tcctatcgat atttttcctt cgtgctgggt atcttgagca gaatccaagg
     2941 aaataattcg taacccattt tgattcgtac aatggatata ctctcccgtt tcattgcttt
     3001 caacactcat tccttcaaag aaaacactgc gacgatcatg attcccacga caaaaatagt
     3061 aaggtgtggt tgggaaatat tgatccaaca ttttccgtaa gacttgataa tcagaaatcc
     3121 ccccttcatg tactaaatca cctgttaata gaataaaatc gtacactgtt aacgagagtt
     3181 cttgtaacaa tttttccaat gccaactgtg gatttttaat tgttggaaat agttcagcag
     3241 taaacaagtt agtttgatag ttttctctaa aatggatatc agagagatgc aaaaagcgca
     3301 tggctattcc ctctttcttc aataaaataa tcaggatatc ctaaattttc ttgcgtctga
     3361 taatcaaaaa aatgcattct ttcaggttct atgcggagat tgactgcatc accaatctgc
     3421 caagtaggtt tttcattaag gacaatcatt tctataccat cgactttaac ggtgtacgca
     3481 taactttgac ctaatagctc acgatacttc acttgaccgt gtaaatcgct tgtttctttt
     3541 gaaaaggtta cgtgttctgg tcttatgcct aaatacaatg agccactaac ctgaagaagt
     3601 tgtttttgcc aagctggatt caatgttaaa aattgactgg ctacctttaa tgttccttga
     3661 tcataagtaa ctggcataat attcatgcct ggcgaaccaa taaaatttgc agtaaaagta
     3721 tttttaggtt tcgtataaac atttttcggc gtatcaatca tctgaattct accttcattc
     3781 aataaaacaa tccggtcagc taacgtcatc gcttcaattt gatcatgagt gacgtagaca
     3841 aaagtttgtt tataacgttg atgaattttt accaattctt ttcgtgcatc caatcgtaaa
     3901 cgcacatcca aatttgataa cggttcatct aataaaaaat aattactccg tttaaccacc
     3961 gcacgagcca atgctacacg ctgccgttgc cctcctgaca aatctttagg aagtcgatct
     4021 tggtatggcg ttaaacctaa catcttcaaa gcttcttcca aacggtgctg aatgtcagct
     4081 gtcttaattt tattcgccct taatgcaaaa ataatattct cctttacagt catgtgagga
     4141 tataaagcat aattttgaaa aaccattgcg atctctcgtt ctccacaagg tagcttattt
     4201 gctagttgtg tacctaaaaa aagctctcca ctcgtaattt tttccaaacc agcaatcatt
     4261 ctcagcaaag tagattttcc tgaaccagat ggtcccagta acacaattcg ttctccagcg
     4321 gccacagcta aatcaatttc ttttaaaatg gtatgttctc catatttttt tccgatattt
     4381 cttaattcaa gcgtttctcc catgatttgt cctccaattt atttaatacc tgcagaaaca
     4441 aaagtcccga taatatattt ttgaaaaatt gcatacaaga ctaacggtac aaacattgta
     4501 attaaactaa tcgccattgc tacggtaaaa tttgtcccac cttcagaact aatataggtc
     4561 tgcatcgcca acggcaaagt ataattatct actgtccgta aaattaatac cggccaaaca
     4621 tactcattcc aagaggtaat aaaaaaccaa ataccggtag aagtaatcgc atgtctagta
     4681 agaggaaaaa caattttact catgacggtt gtttgtttga tgttatccaa tgccgccaca
     4741 tctagaaggc tcactggaat ttcccgcatt gtttgcgtca ataaaaaaat gcccatcgca
     4801 tcagcaaatt gtggtattat cacaccccaa gtagtatcaa ttaaattaag ttttgcaatc
     4861 aacaaataat taggaatcat cgcaacagta aaaggaatga acatcgtacc aatgatgata
     4921 aagtaccaga ttttcttccc tttgattcgg caataagtaa aactatatgc cgcaaaaaaa
     4981 gctacgccta acttaacaac cgttataatt gtagcaataa aaaaagtatt tctcgtaatt
     5041 ttgataattg gaagattttc gaacaaatag caaaaatttt ccaaggtaaa cggctgtggt
     5101 atcaaactag taatcgtctg ataggcatct ttcaatggct taaaagaatt ggccataata
     5161 aacaataatg gatagataat aatgattatg atgaacataa ataagagatg tggcaaccag
     5221 cgtttaattt tcatagtaaa cccccttttc cacaaattta aatattaccc ctaagaaaac
     5281 agcaaatatt aataaagtta aaactgaaat tgctgaagat tctccgactt tatacatttc
     5341 aaaagccttt tgataagcat aatagatcac gtttgcacta ccattattcg gtccaccacg
     5401 agtgatgatc ttaatcggag taaacacata ctgcagacct tgaacaatgg tcatcaccaa
     5461 taaatatagt cctgtcgaac tactcatagg caacacaatt gtaaaaaaaa tgcgccagat
     5521 gggtacacga tccaacttag ctgcttcgat catctcttga tcaattccca caattgctgc
     5581 aaaaatgaca atgaaattat aaccaaaaac tttccaagca gtaatacctg caatcacagc
     5641 aattacccaa ccttgtaaat tcatccacat aggcagtctt aaaccaaaat ttcccaacaa
     5701 gacagcaatt ggtcccgata ccggatttaa tatccatgta aaaaccatcc ccgccactac
     5761 caaagaaatc acagctggaa taaaaaataa tggtttatac aaattctgcc ctttttctat
     5821 aactattcct aataaaaagg atgtaatata tgggaacaca cagctcagca agacaaatag
     5881 aacggtatag gacaaagtat tcttcaacac tttatgaaag actggatcag tcagcacctt
     5941 tacatagttg gcaaagccaa caaattcttt agtgggggcc accatattcc agtcaaaaaa
     6001 actcaaatag atcgtataaa tcaatggata aaaaacaaac acgcccaaca aaataattgc
     6061 tggcgccaga tacaaataac ctacatagcg cttcatttcc gacagcctcc tcatccttat
     6121 gtcaaaagca gacgaccttg tttttccttt cttacaggcg ctgtcgtctg cttgctttgc
     6181 ttatttgagt aactcattaa tttccttttg agtagctttc atcgtttcat tgacggaatt
     6241 acctgataaa attttatccc gcatatccaa caatttttgt tcagcttcta atccagcatc
     6301 tccaggaaat gaactccaac tgaccatctt atccatctga tcaattgctg gtatcatcat
     6361 ctgattttca gctaagaaag cttttaatcc attttgggcg tctgcaacat ctttacgtgg
     6421 tggtaaataa ccagtacctt ctgtccattt agctaaagat tcaacactat aaagatattt
     6481 taagaaatcc caagttgctt cctttttctt atccgtttgt gatgtaacag ctaacattgc
     6541 gccaccagca ggtagtttaa cttttttccc ttcccaactt ggtgactcaa ctgctttagc
     6601 agtaaaagta ctattttttt gaatgttgtc cctttgagca atagtggtat agaccatcgc
     6661 gacgttacca tcaataaagc tttgcacacc ttgttcccac gtagtgtgta atgccgtctg
     6721 gtcttttacc atcctttgat ataattcata agcagcgata ccatctttag aagcaaaggt
     6781 agctttgcca tcttttatta cacttgcccc attactttct aaaattgctt gttgtgccca
     6841 attatcagca ggttcttgaa tatacacgcc atactttcca gttttttctt ttattatctt
     6901 agaaaaagtt tgaacttctt cccaagtttt tggaccattt tcatccaagc ccgctgcttt
     6961 taatatatct tgatttaaat ataaaaccgg attactaatg gagtaaggaa tacccacttg
     7021 tttaccatct ttttcagaca gtgccatgat attgtctaaa aaatgatctt taaaataatc
     7081 agcattatcc ggatccactt cttctactaa agattgagga ttagtatatt tgaaattttc
     7141 tgcaaaataa tctttaaaag cccatcccac ttgtactacg tccggtattt ctttggaggc
     7201 aacagaagct tgtaaatttt gcatcaagcc tttatacata tcaggattat attttgctac
     7261 aaccttcact ttgtcttgag attgattgta tttatctact aattcattca ctgtttttcc
     7321 acctagggta tccgcattaa catgccaata ctctatcgtt acacgaccct ccgatttttt
     7381 tgttgtcttg gtattcccac taccacaagc tgccaaagag atgacactta aaacagacac
     7441 aacccctata acaagatttt tctttaacat aacattaact tcctcctaat ttctttatgg
     7501 ccatttttct aatcaagaaa tattatgaac taccaatgta aatttctagt taattttctg
     7561 taaataattc gaaatttgtg catttcgggg tacagggtca ctggacatat ccatcaagct
     7621 aagaataaag cccccatatg gtcatgtatc ccatctccaa atctcttaac tcctctgttt
     7681 tgattataaa atagtttatt ctgtgctcct ttcttccata aataggccca tgcacaacgt
     7741 gaggttatat tataaacacc acagcgcgca aggtactcaa tcgtttctag ttcattgact
     7801 gtccatacag aaaatgtcaa tcctagctgc tgccccactt cacgggccaa cttagttaac
     7861 catccttcat gaatattgac gatttgaacg ctttgatagg ctaatttctg taaggtatcg
     7921 atcaaatctg cttcggtaat atccccaagt tttttccctt cgaactgatt ttcaatatta
     7981 taaaatatat gctttcccca aatcgcagcc caagataatt gtacatagcc actaagaaca
     8041 atttgtggcc agatttgata ggtttgtgcc agcgaaaaaa cggccatttc caatccctct
     8101 tcttttaaat cacagttaat ggtcatggtt gccttctcat tggccactgc ttgaaacacc
     8161 tgctcaaggg ttaccgaatc tgggacaact tcttacctgt catgggctag tattagcgta
     8221 ccatctgttt gctgacgcac atctacttca aaacaaagct ggcgatccaa acaaaagcga
     8281 agaaatgccc agctattctc ctccgtattg tcactaccag agtgtgcagt gatcatggtc
     8341 attttgaccc ctcctttaat tccgtgattt tctcccacac gtccactata ccgatgaagc
     8401 gttaatttct tgttaatttc ttgtaaattc cctgcgtttt ttcagtaaac tagttgtaaa
     8461 tcttgcatgc tccttcctac gaactcatga atcgtcgatt ttgcccttct agctagtttc
     8521 accaattaag tagttctaca ttccccgctt ttaacctact acaccttttt ttgttttcag
     8581 ctattccttc ttcttgctga aagtccttga ggttctttct tacaggatac catcaaggtg
     8641 ttcaatggct tattcccatt gaatgaccga gacttactat cagatttctg ctcgttgaac
     8701 caagtctttg atacaggatt tcttccgtca tcttctcaca aataccacct tgcgtttctc
     8761 tagcgattgg tcgatgtgca ccctcccagt ggactgtcac tacccctttt tgctatgcct
     8821 agcccacaaa aaaagctgtc ctcacagaca gccagactgt agacaaaagc tgttccaaac
     8881 tcgtaactcg agcttggaac agctttttat ctacttcgtt tcgaattttt gaacaaaaaa
     8941 ataaaggctc cttgcctttc catagccagg tggctaattt ctttaaattc attgcagcaa
     9001 acactagcat cgtatgcgtc gcgacttttt ccaagcctcg atacttggtc caacgcatcc
     9061 catgcttttc ttttgcatcc gcaaatattc gttcaatcgt ttgtttccgt tttttgtaaa
     9121 ttgatttatt cagatctgtt aggcgaagat gttccacttc atccatcaga tctccccaaa
     9181 catgtctcgt gatg
//
LOCUS       DACCYH010000105         8706 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.110, whole genome shotgun sequence.
ACCESSION   DACCYH010000105 DACCYH010000000
VERSION     DACCYH010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.110"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            43..990
                     /locus_tag="IVY85_002530"
     CDS             43..990
                     /locus_tag="IVY85_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8045498.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(1058..1351)
                     /locus_tag="IVY85_002531"
     CDS             complement(1058..1351)
                     /locus_tag="IVY85_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP8045499.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYNPNLEEPTYHFKKQ"
     gene            complement(1379..2044)
                     /locus_tag="IVY85_002532"
     CDS             complement(1379..2044)
                     /locus_tag="IVY85_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP8045500.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(2184..2816)
                     /locus_tag="IVY85_002533"
     CDS             complement(2184..2816)
                     /locus_tag="IVY85_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8045501.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(2823..3890)
                     /locus_tag="IVY85_002534"
     CDS             complement(2823..3890)
                     /locus_tag="IVY85_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8045502.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(3887..4618)
                     /locus_tag="IVY85_002535"
     CDS             complement(3887..4618)
                     /locus_tag="IVY85_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP8045503.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(4787..5266)
                     /gene="greA"
                     /locus_tag="IVY85_002536"
     CDS             complement(4787..5266)
                     /gene="greA"
                     /locus_tag="IVY85_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP8045504.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(5402..6577)
                     /gene="mltG"
                     /locus_tag="IVY85_002537"
     CDS             complement(5402..6577)
                     /gene="mltG"
                     /locus_tag="IVY85_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP8045505.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            6781..7950
                     /locus_tag="IVY85_002538"
     CDS             6781..7950
                     /locus_tag="IVY85_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8045506.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(8217..>8706)
                     /locus_tag="IVY85_002539"
     CDS             complement(8217..>8706)
                     /locus_tag="IVY85_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045507.1"
                     /translation="ILNYSSVVNMVKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKK
                     DQLLLKNHQVTEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKN
                     ATVLKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTS
                     HE"
BASE COUNT         2672 a         1732 c         1356 g         2946 t
ORIGIN      
        1 gtatgcgcta cacttagagt aaagaaaggc gggaaattaa ctatgtcaga aagaatcatt
       61 tatgcagatc gtccatttcg tgatgaattt atcgaagaaa tccatactat agcgcctggt
      121 tatatcttta aaacagaatt acaaccagag gatctacaaa atgtagagat cagtttgggc
      181 tggaactcag actaccaaga aaaactatta gcctcttcta ccttgaaatg ggtccaatct
      241 atctcagctg gtgttgatca gctacccctt gagacttttt caaagaaaca tattttactt
      301 tcaaatggta gtggcatcca cagtgaatcg attacggagc atgtcttagg aatcatcctt
      361 gggtattctc gtggtctttt tcaagcacaa aaggcacaga gtgacatgaa atggttaggt
      421 tcatcggtcc attaccaatc tatccaaggg aaaaaaatac taatcattgg aactggccat
      481 attggaaaaa tgattgcaaa aaaatgcaaa gcattagaca tgaactgcta tggaatcaat
      541 acatccggtc atccagcaga aggaatggaa aagacttttt ctttaaatca actaaaggaa
      601 gttcttcctg aagtagattt cgtagtcaat atcttacctc taacggagga aacaactggg
      661 ctctttgatc agcatatgtt tgaacacttt gatccaaaat ctgtattcat caatgttggc
      721 cgtggtgctt ctgttaagac acaagatctg gtgcaggctt taaataacca gcaactttcc
      781 tttgcagcat tagacgtttt tgaggaagag cctcttccac aagatcatcc tctttggaaa
      841 atggacaatg tactgattac ttcccacatt gcaggtttga cacctgattt tcaaaagaaa
      901 ttaatggcta tttttttaac caatctgaaa agctactttg ccacccatga gcttcaaacc
      961 aatcaagtaa aattgtctgc aggatattaa tagctgaaaa caatattttc aaaagaaaaa
     1021 agagaaggtg acaaaaattg tgtcaccctc tcttttttta ttgtttttta aagtgatagg
     1081 ttggctcctc caggttggga ttataatcaa caagtaaatc atatccttta aagaaccatt
     1141 catctgcttc ctcgataaaa aaaagtgtcc catcaatttc cactttcttt acaggatgtt
     1201 ctggctgttc aactgacatt ccaatcgaaa aaccatcatg aacctctgtt ttgccgtaga
     1261 cttttccaaa aaaacgtatt ccgtaagttt ctggcagatc aatttctgag tcaaaccact
     1321 tttgtgcttt tggcgttacc gttaatttca tcccaacaac ctactttctc ttttctttct
     1381 attgattcat cttatcgtct agaatatcca catcgcctgt ctctccaata accagtagat
     1441 tatcattttc acgaacatat tcatctgctg caggagaagc gataaccgtt tgatctactc
     1501 ttcgtatggc gactactgtt aagtcaaaac gttgcctgaa atttaattca gctaatgttt
     1561 tgttgaaaaa tttaggattg gagactcgga tttctgctaa agaatattta tctgacaact
     1621 ctaggtaatc taaaatattc tttgacacta aattatgcgc aatccttatt cccatgtctc
     1681 gctcgggatg gacgacgcga tcagctccga tcttttcaag tactctggca tgatactcat
     1741 tttgggcttt tgctaaaata tttggtacgc ccatttcctt taccatcaga gtaactaaga
     1801 tactggcttg aatatcttct ccaatagcga caattacatg atcgaaattt cttatgccta
     1861 atgatcgaag tgtcatttca tcctgtgcat ttgccacaac agcatgagtg gctatattca
     1921 tatattcatt gacccgatct tcattactat caatagccag tacttcttga ccagcttcga
     1981 tcagtgttcg gcagatactt ccgccaaatc gtccaagacc tacaattgca aaattttgtt
     2041 tcaccgtttt cctcctttag tatcttcttt tctcagtata acaaagcctt tacaaaaaca
     2101 aaagcaagca tcattattgt aaaaaagcca agaaaaataa tagttatttt ctcagctttt
     2161 gattatcctc tttatcatcg tttttatttc accaaaccat gtttgaatgc atagatagcg
     2221 gcttgtgtac ggtcttccac ttctagctta gccaatatat tggaaacatg tgtttttact
     2281 gttttcagtg taataaacaa ttcgtccgct atttcctgat tactttttcc ttcagaaatc
     2341 agcattaaaa tttcattttc ccgattcgtt agttcttcat gtaatacagg ctcatttcgc
     2401 cgactcattt tttccatcat ttttgttgtc acttctggtt ctaatactcg ctcgccacgt
     2461 tgcgttgccc ttatagcatc agcaatttca tgtgcggtag aggtcttcaa caaataccct
     2521 gctgcacctg cttcaatggc aggataaact ttttcgtcat caataaaact ggttacgata
     2581 atgattttag cttctggcca atcttttaag attgcttttg ttgattcgat accatccatt
     2641 tcttccatga ctaagtccat taaaatgacg tccggtcgaa gtgtcatcgc cttctcatag
     2701 ccttgtcgtc cattttcagc ttctccaatt acttcaatgt cttcttgaat agataaataa
     2761 aaagagacgc ctaagcgcac catttcatgg tcatctacta ataaaacttt tatcatcctt
     2821 atctatgcct ccttcatcaa agggaccttg atttctacac ttgtaccttg acctttaaaa
     2881 ctaatgattt ttactgttcc accaatacca gctactcgtt ctttgatatt gttcaatcca
     2941 taacttcccg ttttcgtttc attcatatta aaccctgttc cgtcgtcaat aatacgtaac
     3001 aaaagattat tgtctatttt gtgcaaatat acctctaatt cattggcttt agcatgtctt
     3061 aatgtatttg ataacaattc ttgaacgatt cggaacaagt gatcctcaat ggaactagat
     3121 agttttacat cttctacatc ccatttcagc gaaatctgga ttttattttg caattccttc
     3181 aataactgct cgataccttg ctttaaactt ttctcttcta aattgactgg acgtagatga
     3241 agaagcagcg cacgcatctc agactgggaa gcgttgatga tctctgctac cattttcaac
     3301 tgcttgcgaa acatttctgg tgtctcgctt ttttctgctt gctccgtcaa tgccgacatc
     3361 atcatcattg ctgcaaacaa ttgttgcgaa actgaatcat gcagctcacg ggctaatcga
     3421 tgtctttcta attctaaaat ttcttctttg gtttgtccat ctacatattg gggacggctc
     3481 gttacaattt gtaattcact agatacttca atcatttttt cttttatctt agtgatttcc
     3541 ttatcaatat ctttgatgta taaatcgtcc gatgcacttg ggatcggaag aagcaacaat
     3601 ttggattcat aattgccaga agaaagtgca cgcagctttt cttcgatttt cccgtatttt
     3661 gtcttttgaa taagtgataa taatagaaag acaattaatc ctacacctaa ggaaatggct
     3721 aacaaatgga agataagcgg aacatataat aaacgtgcac gcagtaattc tccccaccaa
     3781 tgactttgat tgctggcata aaaataagtg aaaagtgaaa atagaataat aaggaaagcc
     3841 gcaatccccg aataaacagc tagcatcgct ctggatattt ttcccatcat attcgtatca
     3901 cctcgacatc tccaatcaag gtattagtaa taatcttcaa tctacgaggg ttttcatcat
     3961 aatcactgct gtagattttg atttgttcat ttttcaaaga aaattgctct tcttcaaaca
     4021 agacatttcc tactaatgtc gcatgctcta atcggatggc tacgcccaaa ggtactaaga
     4081 tccgtgttct gcctatccct tttcttacaa tcacaatatt atcatctttt ggcaaaagcg
     4141 tattgcccaa atctatgata gtatcaccag aaatgacggc gatgttgata tcgtcccatt
     4201 catagacgtc gtttccaata cgttggttac cgaataattg ctgtcttttc cgctcattat
     4261 tatgtgtttt taattgctct gtttggatca tcatgatctg tttctttcgc caaaaagcat
     4321 ttttggttaa atctatccct gaaatctcta cacctttcag accaataaat aaaacggcaa
     4381 atacagccat catccataaa gcaggactgt tgactaagct aaaaaaaatg accaaacttc
     4441 ctaatatcaa ttgaaaattt tgaaatttag tacgaggatg tttgcgcata gctagatata
     4501 tattgaaaat accgaatatc actaaaagca aaagtccagt attgtttacg atttgccaaa
     4561 cagcaaataa cagcagtagt gcttcaacta ccacaaaaaa acgccaagaa ctattcatcg
     4621 ctctttctcc ctcctgacac attctcattt catcatacct atttttttaa ctgttggcta
     4681 taggacttta gatgtaagat cgtttgttga aatctaaaat acacaaagag aaaaagatct
     4741 agagtgtatc atcagtaaga tgaactctct agatctttca gtcagactac gcttgttcta
     4801 ctttgacgat tttaactaac atatcgccac ctggagtagc aatagttact tcatcatttt
     4861 tacgtttgcc aatcaatgct tgcgcaatag gagaatcatt ggaaattttg ccagaaaacg
     4921 gatctgcttc agcgcttccg acaatcgtgt actcttcttc ttctccatct ggtagttcga
     4981 tgaatgtaac tgttttacca attgatacct catctgcatc tacaccgtcg ttatcgatga
     5041 tttgagcaaa acgaatcata ttttcaattg ttgtgattct tccttcaaca aatgcttgtt
     5101 catctttcgc tgattcatat tcagagtttt cggaaagatc tccgaaaccg cgggcaattt
     5161 tgattcgttc aatgatttct ccgcgtttga ctgtttttag ttcttctaat tctttttcta
     5221 atttttcttt accttctaga gtcattggat aaactttctc taccatgtgt aacgtctcct
     5281 tttttcgata aaataccgca aaaggtaatc ttttgaggaa tatagtccaa aaaagattac
     5341 cacgcttttc ttcaaatgta aatgtttttc taaaaatgat caatgtctag agcctgtttt
     5401 tctaagaatt atttacatat ttttccacta attccatatg ttcttcataa gttttagcga
     5461 aataaacatt tccagtgctc gtatctgcta cgaaataata atagtcattt tctgctggtt
     5521 ctaatactgc cttgattgcc tcttcgcttg gattgtcaaa aggtccaggt ccatagccgg
     5581 tattggtata caaattatat ggtgaatcta cagctgtatc ttcatacgtc actaattctt
     5641 tgtgttcacc taatgcgtat aaaacagaga tatctgactg aagtggcata tttgctttga
     5701 ttcggttaaa gaagacttgt gcaatgtttt tacgatcatt ttcttttacg ccttcttttt
     5761 caactagaga agctagagtt aaaacttctt gtacagtcaa atttttctgc ttgatagctg
     5821 aataatagtt ttgcatgacc gtattcgtct tgttcaccat ttcaatcact agatccttaa
     5881 gcgaagtact cgtgtaataa tcatatgtgg ctgggaataa gtaaccttcc aaacggtatt
     5941 ttgtgttctc agctttagat gcactttcca acagttctgg gaatttttgg tgcaactcgt
     6001 taaagaaagt ctcatcattc actaaatcta aaaattcttt cttgtcttta ccagttacct
     6061 tcgctacccg ttcagcaatc tgatcgatat cgtacccttc tggaatggca attttcccat
     6121 ctgcaacttt tgttggctca gaagtaccgc cttcttgcaa ctgtttgcca atctcgtcaa
     6181 gagtcatatt aggcgctaac tgataatagc cagcttgaaa gccagttaaa tttttaaatt
     6241 ttgtataata attgaaaaca atcccgcttt taataatatt gtccttctcc aaaatctcac
     6301 caatctgctt gttagaagag ccactcggga tctcgacttg gactaattgg tcatctgatt
     6361 tatctaaagg cttcaacccg ctatctacat aacgatacac ggtaaatcca agaaatccgc
     6421 caataatcaa taatgtaaga gcaatcacta aaatgatctt cctcacaatc ttatcttctt
     6481 ttttacgcag atttctccgc ttgtcgatta tatctgctgt agctgcctta ctggcttctt
     6541 ttttatcttt acttgggtgt tgtttgtttc ctgacaaaac aactcctcct tactatcaag
     6601 ttcgtaactt tattcattat acatgatatt tgaattggcg tgaatgattt tatttaaata
     6661 ttctatcaaa aatgtaagta gtaaatccat tactataaga ttaaaataaa tgttatcaag
     6721 atacaagcta ttttagcttt attctttctg taaaaaagaa aggagactaa ataaactatt
     6781 atgaaaaaag gaaaacatgc taaaaaaaga aaatattcat ttttaatgat cgttgtcgtg
     6841 ttgtttagcc ttagtgcgtt ctctacagga actattcttg gatggcaatt ttacaataat
     6901 gaacactctc agatagttgc ccatgctcaa tcagctcaaa aaaagttgat actgactacc
     6961 aatgaacaac agctcgttga aaaaatcgat caactattaa gaaaaaatga ttatattggc
     7021 tcaatttacg taaagaaaaa taatcgcatc atcatggaaa aaggctatgg ctatgcgaat
     7081 aaactcacgc aacaaccaaa tgatccttct ctttattatc agattggttc ggttcaaaaa
     7141 gcattgacta ctcttttgat tttaaaacaa gtcgagcgtg ggcacctttc tttagatact
     7201 aagctggctc aattctatcc acaaattgct ggcagccagc aaattactat ccgtgacttg
     7261 ctgtatatgc gctcaggctt gcaccgaact gcttctccaa ccataccgat gactgatgaa
     7321 gaagtcgttc aatttgcgat tcagcactta aaatttacag attatcagac ctctcattat
     7381 gattcattga actttaccat attgactgga attttgatca aattaacaaa tcaaccgtat
     7441 gaaaaattat tgaccgatga tatcatcgaa cctttaggat tacagcatac aaaattttat
     7501 gaccaagtca aaaattcatc caatcatgca ctatcctatc aaatgtctaa agaggatgat
     7561 tactttaaac cactgacaga atctgaaaca gagatccata acgaattagg gactggtaat
     7621 atcagtatgt ccgtctatga tttaaatacg ctgtttacac aagttttatc tggaaaacta
     7681 gtttccaaag acttactctt ctcattatgg cgtgaaaatg cgaatggaca tccatatagt
     7741 ggtggggtat atagcggaga taattatatc ctgtcgcaag gaaatatcga ctcttttgat
     7801 acatctgcta tatttagcaa agattcacag gatgctatca tcatggaaag caatataaaa
     7861 gctgacaaaa ctatcaaact gcctactgct gatctaagaa accaaatcta tgacctgatg
     7921 gaaggaacta ctaagttggc aaatagctga tatgatgaaa agatagcaaa tactacttct
     7981 ttgaatagca tttgcttaaa aaaagtactc gactaaacgt aggaaagagt gtgggacaaa
     8041 tctaaaaatc aatttttgct acagacttta attccaaata aacagaacag aaagaggtgt
     8101 tcttctttct gttctgtcca aattattcta agatttctct ttaccaacat tatttgacaa
     8161 agggcctatt aaatctaaaa tagtatcttt agattgctaa aatatgtgct agcaaatcat
     8221 tcatggcttg ttcttatttt taattttatg caataaaaaa gaagaacaat tagcgtgatt
     8281 atagaaataa ataatgagat tttagaaaaa aatgtgtatg aatacctgat tgttacttta
     8341 tttattccac tttcactttt tactgtaaca aaaggacctc cttgttttag tactgtagca
     8401 ttttttgaat taacagtaac cgttaatcct ttataccgga atacaggtaa aactattgtt
     8461 ttgccttttg aagatttaaa cgtaaatgta atttcatctt tatttggaga aaagctacta
     8521 taaataactt gaccatcttc tgtaacttga tggtttttca aaagcaattg atctttttta
     8581 tagcctatag gtacataatc aaaattctca aattcttcaa gtttattttc catttgttca
     8641 ttagaataag ttccttgaaa attcatttct ttcaccatgt taactactga tgaataattt
     8701 aaaatt
//
LOCUS       DACCYH010000106         8660 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.111, whole genome shotgun sequence.
ACCESSION   DACCYH010000106 DACCYH010000000
VERSION     DACCYH010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.111"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(258..2639)
                     /locus_tag="IVY85_002540"
     CDS             complement(258..2639)
                     /locus_tag="IVY85_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8045508.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2629..3276)
                     /gene="recU"
                     /locus_tag="IVY85_002541"
     CDS             complement(2629..3276)
                     /gene="recU"
                     /locus_tag="IVY85_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP8045509.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3340..3879
                     /locus_tag="IVY85_002542"
     CDS             3340..3879
                     /locus_tag="IVY85_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP8045510.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3969..4388
                     /gene="gpsB"
                     /locus_tag="IVY85_002543"
     CDS             3969..4388
                     /gene="gpsB"
                     /locus_tag="IVY85_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP8045511.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4410..4777
                     /gene="rnpB"
                     /locus_tag="IVY85_002544"
     ncRNA           4410..4777
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IVY85_002544"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4986..6152
                     /locus_tag="IVY85_002545"
     CDS             4986..6152
                     /locus_tag="IVY85_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP8045512.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6198..7694
                     /locus_tag="IVY85_002546"
     CDS             6198..7694
                     /locus_tag="IVY85_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP8045513.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7775..8458
                     /locus_tag="IVY85_002547"
     CDS             7775..8458
                     /locus_tag="IVY85_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8045514.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2724 a         1571 c         1840 g         2525 t
ORIGIN      
        1 aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta taaacaagta
       61 aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt taagctaaat
      121 gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat acagcttggt
      181 acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc acgcttgtag
      241 acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg
      301 tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat
      361 gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc
      421 tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta
      481 gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag
      541 ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct
      601 ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt
      661 gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac
      721 gattctgaag tgacaggcgt cattttttta ttgtaacctg tccacacgga aatcgaataa
      781 tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt
      841 gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg
      901 atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata
      961 taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg
     1021 tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa
     1081 gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct
     1141 tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt
     1201 tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca
     1261 ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag
     1321 tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca
     1381 gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa
     1441 ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg
     1501 tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca
     1561 acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt
     1621 ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc
     1681 ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta
     1741 gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct
     1801 tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct
     1861 cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc
     1921 atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac
     1981 atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag
     2041 taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct
     2101 tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc
     2161 aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca
     2221 gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc
     2281 gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt
     2341 tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta
     2401 gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct
     2461 acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat
     2521 aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca
     2581 ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt
     2641 cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg
     2701 aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg
     2761 tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt
     2821 caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg
     2881 ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga
     2941 tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt
     3001 atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt
     3061 aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc
     3121 gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt
     3181 gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt
     3241 ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca
     3301 ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc
     3361 tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc
     3421 caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa
     3481 tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa
     3541 ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt
     3601 aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat
     3661 gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt
     3721 tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa
     3781 acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg
     3841 ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat
     3901 caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag
     3961 gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa
     4021 caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag
     4081 attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg
     4141 caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag
     4201 aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg
     4261 aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac
     4321 ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc
     4381 aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca
     4441 ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa
     4501 accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat
     4561 gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga
     4621 acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa
     4681 gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct
     4741 ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag
     4801 gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa
     4861 tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat
     4921 ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat
     4981 tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag
     5041 cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg
     5101 cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag
     5161 atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa
     5221 acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg
     5281 gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag
     5341 caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa
     5401 ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg
     5461 atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat
     5521 tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat
     5581 tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata
     5641 atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag
     5701 aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag
     5761 atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag
     5821 aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg
     5881 ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag
     5941 aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga
     6001 gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca
     6061 aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc
     6121 gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat
     6181 gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc
     6241 ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag
     6301 ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa
     6361 tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg
     6421 aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac
     6481 cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa
     6541 aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat
     6601 taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc
     6661 tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta
     6721 aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag
     6781 actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg
     6841 aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga
     6901 ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa
     6961 tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg
     7021 ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat
     7081 cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt
     7141 tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt
     7201 cattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc
     7261 tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg
     7321 ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa
     7381 atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc
     7441 cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa
     7501 tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag
     7561 agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac
     7621 atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat
     7681 atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt
     7741 tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga
     7801 ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag
     7861 agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac
     7921 gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga
     7981 ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc
     8041 gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga
     8101 actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat
     8161 cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga
     8221 actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa
     8281 tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga
     8341 agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg
     8401 gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg
     8461 gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac
     8521 ttctggaaca ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga
     8581 acaatttcaa aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg
     8641 gcaggagaac accgtttatt
//
LOCUS       DACCYH010000107         8586 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.112, whole genome shotgun sequence.
ACCESSION   DACCYH010000107 DACCYH010000000
VERSION     DACCYH010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.112"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(45..1511)
                     /locus_tag="IVY85_002548"
     CDS             complement(45..1511)
                     /locus_tag="IVY85_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP8045515.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1898..2494
                     /locus_tag="IVY85_002549"
     CDS             1898..2494
                     /locus_tag="IVY85_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8045516.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(2552..3034)
                     /locus_tag="IVY85_002550"
     CDS             complement(2552..3034)
                     /locus_tag="IVY85_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045517.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3140..3337)
                     /locus_tag="IVY85_002551"
     CDS             complement(3140..3337)
                     /locus_tag="IVY85_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045518.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3632..4390
                     /locus_tag="IVY85_002552"
     CDS             3632..4390
                     /locus_tag="IVY85_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP8045519.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4450..4878
                     /locus_tag="IVY85_002553"
     CDS             4450..4878
                     /locus_tag="IVY85_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045520.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4924..4996
                     /locus_tag="IVY85_002554"
     tRNA            4924..4996
                     /locus_tag="IVY85_002554"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4957..4959,aa:Gln,seq:ctg)
     gene            complement(5146..5517)
                     /locus_tag="IVY85_002555"
     CDS             complement(5146..5517)
                     /locus_tag="IVY85_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8045521.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5732..5917
                     /locus_tag="IVY85_002556"
     CDS             5732..5917
                     /locus_tag="IVY85_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045522.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6000..6188)
                     /locus_tag="IVY85_002557"
     CDS             complement(6000..6188)
                     /locus_tag="IVY85_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045523.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6212..6475)
                     /locus_tag="IVY85_002558"
     CDS             complement(6212..6475)
                     /locus_tag="IVY85_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045524.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6633..6890
                     /locus_tag="IVY85_002559"
     CDS             6633..6890
                     /locus_tag="IVY85_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP8045525.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6961..7152)
                     /locus_tag="IVY85_002560"
     CDS             complement(6961..7152)
                     /locus_tag="IVY85_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045526.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7293..7613)
                     /locus_tag="IVY85_002561"
     CDS             complement(7293..7613)
                     /locus_tag="IVY85_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045527.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7636..8070)
                     /locus_tag="IVY85_002562"
     CDS             complement(7636..8070)
                     /locus_tag="IVY85_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8045528.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8405..>8586
                     /locus_tag="IVY85_002563"
     CDS             8405..>8586
                     /locus_tag="IVY85_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8045529.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2719 a         1694 c         1361 g         2812 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga
     1741 aaatccttac ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg
     1801 tgagcatatc tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa
     1861 aaaaatgcaa aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt
     1921 gaaaaaccgc cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt
     1981 aacaactttg atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac
     2041 acgcgcagtt atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc
     2101 acttcgccca ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt
     2161 caaaaatggt tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca
     2221 agaacaattt gaattatatg cagacaactt cccagattgg tcagaacaat caaacggaat
     2281 cgcaacagct aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca
     2341 ttacaaccca gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa
     2401 actacgttcg caattagtat tcggttcacc agaaacaccg gctgaggaaa aagaatacat
     2461 gaacgatgcc gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt
     2521 tgtgacaaac atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct
     2581 ctgcctcatc gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa
     2641 atttcagcat cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac
     2701 cttttggctg atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa
     2761 agctattcaa aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa
     2821 aaatcattgc tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg
     2881 tataccaaat gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca
     2941 cagtccgata aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga
     3001 ataaaaacaa tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc
     3061 cagaaaatag gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc
     3121 gcacgtccaa ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt
     3181 tttcatctaa taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt
     3241 ttgtcatttt tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg
     3301 catattgttc tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga
     3361 acttttctcc acattaccac tgctatttct ttggagattt aactcattga tttttattta
     3421 cagttaaaaa tcaaccttac aggcattata gcgtactgtt ttcgttttca caattaaaac
     3481 agctgttgta tttagaagaa tttttttcaa aaaattaaaa catattcctt aaaaactaat
     3541 tatttcctac atattaaaat ataatacttc catttccaat aaaaatacat ttataatagg
     3601 aaagataaac agctgaaaag ggagatttca aatgaagaaa aaaacgttca aaaaactatt
     3661 gtttctaggg ggcacagctg ctcttttatt aagcggtaca agttattacc tacatatcca
     3721 ttctccagaa catacaatga acactgcaca aaaaagttca acacaaaaaa cgaaaaagac
     3781 aaaagagaaa gcagaagttt tactagatgt tccattagag agccagtttg attctccttc
     3841 tttagaaaat ggctgtgaag tcacttcgct ttctatgctt ttatcttttt atggttatga
     3901 aacaactaaa aaccagttag ctgagcaatt ggattacgtc cctgttttca atgcagatgg
     3961 gactcatggc gatcctaatg aaggatttgt tggtgatatc agtggcggag actgggctat
     4021 ggtcgtctat gttcccccag ttgcctccct tgctcagcaa atcgtccaaa cggattatca
     4081 cacttttcca aaaaccgatg ctcaactcga tgatatcaaa aaagcactat ctaaaggaaa
     4141 tcctgtctgg acttctgtga ctatcaattt tgaagtccca gatgaaactg attttatgac
     4201 ttggaccaca aataatggag aagtaagagt cactcctctt gttcatgctt gtgtagtcac
     4261 tggatatgac aaagaaaata tttatgtcaa cgatccctat ggtgtaaaaa atcgagcagt
     4321 accgatcaac gatttttcgg cgattttcac tgcaatgggt ggacagatgc taactttgga
     4381 gaaaagctag taattcaaac aaaaatccct tatactcaag ataccaaacc tttgaaggga
     4441 gcgtagataa tggaaaaatt cacacataaa aaaatggatc caaatgaaat tccgattatt
     4501 tttgtccgtg actgcaaggg aaatgttcaa ggtaaagtct ctattaatga gtggaacgag
     4561 cgtcgcagac ctgctaccct caatgaactg gaaatcaaat tgtatcgtca atctcttgtt
     4621 tattacgcag atcaagagta cgagaaagcc accgatttat tgaaatttct aattgctcga
     4681 acagagtata ctcgctttga atatattgaa cgtttagcaa acatctatca tattatgaat
     4741 gaacctgtca aagaatatca gttattggac accgtcttgt cagtagcaga actgattgcc
     4801 cttcctgctg gattagaaaa gaaactagta cgccgccttt tacgagtaaa gcaacaacta
     4861 tccgatcaag aaaaataact tgcgtttttt taggaaagtg ctatgattat tttgttgaat
     4921 aaatgggatg tagccaaact ggtaaggcac ttgactctga atcaagcaat tgtaggttcg
     4981 aaccctgcca tcccaatttg atgtgcttgc tgaaaaaatg aaccccctgt aaaagaacaa
     5041 tctttttgca gggggattat tgtattattt ttactacttg tatttagttt acataattaa
     5101 attatgaata ataaaatcgg gcgtattctt aatacaaaaa ctgatttacc caataattgc
     5161 taatgctttc tccggaactt tttctctagg aacttcctca tacgtttcta tgtgtgctcc
     5221 tttattgcta attttcaaat aatgattttc ttttaacaca gaaagaccat taaattcaac
     5281 cgttcgttct ttcccattgg catcagcagc tttctgcaca taccttgctg taccatattc
     5341 atttacttcc tctggttttt gtgtctttac atagacctct cctttttcaa ctaatggatt
     5401 tagctgatca agtataccag ggatttctcc ataacttcct tcagtataat atttcagtcc
     5461 aaatagcaaa ataccactaa tcgccgccag tccaatcaaa tatttaatca ctttcatctt
     5521 tctacctcct tgttatatat tcagtatatg ggtcagttaa agaaattcca tccgtcccaa
     5581 gttggagatt tttctcctac tttagaagcg agaatcgagt gtttttttag taaaaatgat
     5641 cgttatttga tattattgac gtgtggataa atcagttttt ctcgttttgt ccatgtaaaa
     5701 aatcatgttg atagcaaagg atgtgttcaa aatgaatcaa gaagaattat ttcaaaaagt
     5761 caaggaaatg attaagaacg gcaatttcga tggcgcaaaa cgatttatcg aagaacataa
     5821 agaacaatta ggaccttata aagagaaagc ccaaaatctt ttaaaagatg taaatatcga
     5881 cagtgtcaaa aacaaattta aaaatctatt caaataagta aaactttcac ttttgtaaaa
     5941 tcaaaggagg acaatgctgc tgcatcgtcc tcctttggtt cttttctgaa tacttcatac
     6001 taaaataaac gtgtcggatc gtaatgataa gtcacattgc cgttcagtga ggcttttccc
     6061 accacatatt tacgattctc caacttcagc cacgcttctc gaaaagccat taattcttct
     6121 ctttttacat caatcttttc gatttttcca tcgatcaaat cttgaataag ttgttcatat
     6181 ggattcattt ttttcctcca tctgctgtca atcaataagt tgtaaaaata ggttcatatg
     6241 cttttttggt atctagatct tctctgactt tgactgtcgt ataaataatt ttttctactg
     6301 ggatactata ataacttcct tcaccattat tgaatcggtg aacgtctttc tttgctaaaa
     6361 taccatttat tttattcgtc agtacacgtt gttcgacact gttacttaca acatagtaaa
     6421 tcacccgaat attattgatc aaatgcaaat cgatttctaa cactacatga tccatgaaaa
     6481 tcccactcct tctttcactt tgattatagc aagtttctcc tctaatttca aaatcgatac
     6541 agattttacc ggataaatcc acaaaactgc ttgcgccatt tcgcacaaag tgttattttg
     6601 tatcatggac tttatttatc ggaggcaata gtatgatcgc gatcggtcaa tttgtttttt
     6661 atattccttt ttttattatg ataagcattc ttttctatta cataaaatgg acaaaaaaga
     6721 agttttcagt tttactggcc tctttacctg ctgtatattt tacttatcaa attttttctt
     6781 ttaggcattg ggaaacaacg tctgtattaa taacacacat catcgaattg acgctttcag
     6841 taatttttct tattatttgg atctattttt tatataagaa tcagaattga atccctcgca
     6901 tgtttaaaaa gaaaacgatt aaagatgcag caaatttctg ccgatcttta atcgtttttt
     6961 ctattctgtt ataccaaaaa aatgtttgaa tcggtgaagg atcgttcctt gttttaattc
     7021 tataaattct tgtgcgatcg ggtacttttc ttccaaattt tctttgttct tttcgatcag
     7081 gcttttcgct tcttcatatt ttttatctgc acatagtgct tttacttcct gtaatacttt
     7141 tcctgcttcc atttgcctca ccctcttgcc attttattgc tttaagtttt atttaagtta
     7201 tacgcattat tctagtttca tcccaaataa ccttttttca tttttactcc tcgaactaat
     7261 cagttttcta aaggctgatt ggttcgtctt ttttattttt cctccatttc attctgctct
     7321 tctgttaatt tatctaatgc tttgattgat gctgcggctc ttgcataatc atagctgtat
     7381 aattccataa atttatcagg cagaacaaac cacgctggat gataaccgtt agctgtgaca
     7441 gggaatcctt tatcttgttg gttatctcca tcccatctca gagcaacacc tttttttcct
     7501 ttccacttta ctaaagcgat cgagtatccc ttctttgttt gtgtgccgtc ttcaataatt
     7561 gctaaaattt ctattttttc ttttggcgca cggacttctt gaggacgaac cattttctat
     7621 tcctccctta tctcattact tcacgaccaa cacactgcaa ggggcatgtg atacgacata
     7681 agaagcggtc gatccaacta atgcttgttg tatagcacct tttcctgtcg ctcccataac
     7741 gatcaaatca atattttctt gtccagggat gacattggca atcaaatttc ttggatctcc
     7801 agtttcaact attgcgtgga tctcagcaat gccgtactct ttggcatcat tgattttttt
     7861 cagcatggct acttctgttc tttgtttttc atctgcatag atttttgaaa aggcataggc
     7921 actatgggtc agctcaactt tgttgatgat tgacacgata aataagtggg cttcattgtc
     7981 ctttgctaag tcaagggctt cagcgaacgc tttgatcgat tgtcttgatc cgtcaactgc
     8041 taccatgatg ttctggtatc ttttactcat tggaaccgcc tcctttttct tttattgtaa
     8101 catctcaagc aaaaatacaa aaaatatacg caatatgtaa ataaaatgat gaacaaaagc
     8161 aatttctttc aagttattta tacatttctt ttctcatcag aacacctgtt ctaatcccat
     8221 tatttctgca ttatgtgaaa ttcctcatca tttttcacat cgagcgaaca tcattctgtt
     8281 acaaaaaacg tggatttgtc ataaattgtc atgcttcttt tacctatgaa atattttcac
     8341 gtgttaaagt attcctcgtt gaacacaaca aataaaactt tttaacgaat aaaggagatt
     8401 tttcatgaca tcacttaaaa ctttactttt tggaacaact ttggctgccg gcgctgcatt
     8461 cttcatggga acgactgctc atgcggacga agcttatacc gttcaatcag gtgatactct
     8521 atcaacgatt tctcaaaaat acgttggtga taactccttg atcaatgcca ttgcagaatc
     8581 aaattc
//
LOCUS       DACCYH010000108         8573 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.113, whole genome shotgun sequence.
ACCESSION   DACCYH010000108 DACCYH010000000
VERSION     DACCYH010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.113"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            20..538
                     /locus_tag="IVY85_002564"
     CDS             20..538
                     /locus_tag="IVY85_002564"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8045530.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            666..1349
                     /locus_tag="IVY85_002565"
     CDS             666..1349
                     /locus_tag="IVY85_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP8045531.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1658..1731
                     /locus_tag="IVY85_002566"
     tRNA            1658..1731
                     /locus_tag="IVY85_002566"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1692..1694,aa:Arg,seq:tct)
     gene            1890..2249
                     /locus_tag="IVY85_002567"
     CDS             1890..2249
                     /locus_tag="IVY85_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP8045532.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2372..4003
                     /locus_tag="IVY85_002568"
     CDS             2372..4003
                     /locus_tag="IVY85_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP8045533.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4065..4706)
                     /locus_tag="IVY85_002569"
     CDS             complement(4065..4706)
                     /locus_tag="IVY85_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8045534.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            4988..5296
                     /gene="rplU"
                     /locus_tag="IVY85_002570"
     CDS             4988..5296
                     /gene="rplU"
                     /locus_tag="IVY85_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP8045535.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5323..5667
                     /locus_tag="IVY85_002571"
     CDS             5323..5667
                     /locus_tag="IVY85_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP8045536.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5680..5973
                     /gene="rpmA"
                     /locus_tag="IVY85_002572"
     CDS             5680..5973
                     /gene="rpmA"
                     /locus_tag="IVY85_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP8045537.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(6066..7214)
                     /locus_tag="IVY85_002573"
     CDS             complement(6066..7214)
                     /locus_tag="IVY85_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8045538.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
     gene            complement(7226..7537)
                     /locus_tag="IVY85_002574"
     CDS             complement(7226..7537)
                     /locus_tag="IVY85_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045539.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            complement(7590..7985)
                     /locus_tag="IVY85_002575"
     CDS             complement(7590..7985)
                     /locus_tag="IVY85_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8045540.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            complement(8021..8365)
                     /locus_tag="IVY85_002576"
     CDS             complement(8021..8365)
                     /locus_tag="IVY85_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8045541.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
BASE COUNT         2578 a         1427 c         1656 g         2912 t
ORIGIN      
        1 aagaaagaag ttgaaaacga tacctgatta caaaaaaata acttttataa gttctataac
       61 gttgattata tttttaattc ttattcttct cgtcgtcttg gttccccgaa tttttggttt
      121 gactccttat attattgagg acaaagaaat gagtccatat tatgcacaag gaaatctgat
      181 atatgtacga ccgaaaaata caaatgagat tttagtcgga gatattatta catattatga
      241 aaattcagga aaaaaaatct cgacgcgtcg cgtaatagcc gttgaaaaca accacgaaga
      301 tttttataca aaagctgata caaagactta tattgagcct ggagtagtaa aaaagagaaa
      361 tattatcggt agtcccatct ttcagattcc atatattgga ctagtccttt caaaaacggc
      421 atttgcatgg attcaggggt tattctttac gattgctttt tgcctaacag gaatcacagc
      481 atggaatacc tttgtagagt tgaaaaagaa gcggagacga acacagaaat ttttgtaaga
      541 atgttaaaaa cataaatgta ttccagagat ccaacgactc ttctgaacat gaaacacgag
      601 aaaacactca tgcagcttgt agatttagtc agttgttcgt ataatgtgtg aagaggagga
      661 gtagtatgcg caatgaaatg atgcatcgtc ctgtagtcaa agaagaacta ttggatttta
      721 tgcgtaccca gcaaaaaaaa ttaccgggtg agctaggcaa actggaagaa gaagctcatg
      781 tagcagaagt accagttatt ccccatgaaa cagttgtatt tttgaaattt ttacttggac
      841 agcttcagcc tgaacgaatc ttggagatcg gagcagctat cggtttttca tcaagtgtca
      901 tggccacgac aatcggagaa aacgctcatg ttacgacaat tgatcgtttc gatgtcatga
      961 tcgagaaagc taaaaaaaac tatgaacgtc taggactgac agataaagtg actttgcttg
     1021 aaggtcaagc agcagatatc ttgccggagt tgtctggtcc gtatgacttt atttttatgg
     1081 atagtgcaaa atcaaaatat attgaatttt tacctgaatg cttacgtttg ttaagaaaag
     1141 gtggcgtttt gatggtagat gacatcttcc aaggaggaac gatcttgctc ccagatgaag
     1201 agattccccg tggcaaacga gctatccatc gtaaattaaa tgaatttctt cgtgttgtga
     1261 tggatcatcc cgatttgaca tcgacaatct tgccattagg tgatggtgtg atcttaatga
     1321 caaaggaatc agaaacaatc gagttgtgat acatgattat ggcagcctgt agataatcga
     1381 ttgaatcgag ggctcaattc gaacttgatg taaaagtttc tatatttgta agtcaattaa
     1441 aggatacagg aaatctaaat aaacaggagt agcatgaagt caatgtagct cctgttttca
     1501 attgataacc ttagaacagc tgaaggagaa tgtctcgttt taaaagattg atttagtagt
     1561 tgggccaaaa taattcatta tttgctaatt aaataactat ttcgtattga cagaagcaat
     1621 ttaattatct ataattattg atgttccgct gattcttgtc atagtagctc agctggatag
     1681 agcatccgcc ttctaagcgg acggtcgggg gttcgaatcc ctcctgtgac gctaaaaaac
     1741 cgttagaaac tgaggtttct aacggttttt ctttgttttt tgtgccaata ttgtttgttt
     1801 tcaaaggcaa ataaaataaa aaaacatcct tatcgagtac aattataagg agcaaaaaaa
     1861 taaaactcat cagaaaagag gaagaagata tgacgatact atctagaatt ttagtgactc
     1921 tagttgcaat tgaatttttt tatatcatgt atatcgaaac ggtgagaact gattctgaca
     1981 cgacaagtag ggtttttaaa atgagtaaag aagagttaag cagaaaatct gttcaaacat
     2041 tgtttaagaa ccaaggagtg tacaatggac tattaggagt gggactactt tatggagctt
     2101 atctttcttc cgcatctaaa gaaatcacaa gtatgctatt aatatctatt ttttttgttg
     2161 cattatatgg cagtttgact agcgatcgtt ggattttact caaacaaggt gggctagctc
     2221 tgttggcatt gctttctatt ttcttataaa tatagaaaaa ataagaattt atctaacagg
     2281 aaactttgtg aaaaatatta ctttatctca tgaaatatga tattctaatg aatggtattt
     2341 tagaaaattt catgaaaaag gagttagata ggtgttcaaa aattatgttt cctcactaaa
     2401 aaaagaattt tcaggttatg atctttcaag atttcagaaa gatcttttgg ctgggatcac
     2461 agtagctgct gtagcattgc ctttagcctt ggcttttggg gtatcaagcg gagctgatgc
     2521 ggccgcaggt ttgattacgg ctatcattgc cggtatcgtt attggcggtt tatcaggcgg
     2581 tttttatcaa atatcaggtc cgacaggtgc gatggcagct atcttgatgt ctattgcggc
     2641 aacaaaggga atgcaaggtg tattgcttgc tactttttta gcgggtctat ttttacttgc
     2701 agcaggactt ttccgattag gaacactgac ttcttttatc ccagcacccg taattacggg
     2761 gtttacttca ggtattgcta tcattattgc attaggtcag atcgataatt tatttggtgt
     2821 tcactcggaa ggtacaaacg ttattgaaaa actaagtagt tatcaaacga taggatttcc
     2881 gataaacaca gcctctttga tcatgggagg gctagtcatt ttagggatgt ttatctatcc
     2941 aaaaaaatgg gcgaaacgaa tgcctagttc gctgctggct atcattttgg caacagcagt
     3001 tatgctattg ttccatttac cagtagcaac tgtgggtaag attccacaaa cattgatcag
     3061 tagtaatcgt ttgaatatgg gagattttag cttttctgcc ttgcaacaag tggcagttcc
     3121 agctattagc attgctttat taggaatgat tgaaagcttg ctatgtggtg cttcggctgg
     3181 ccgaatggca aataagccct tagatagcaa tcaagaatta atggcgcaag gaattggaaa
     3241 tctgttgctt cctttctttg gcgggattcc tgctactgcg gccatcgccc gtacaagcgt
     3301 ggccatcaaa tctggtgcac aaactagatt agctggtatg atacatgcag ttgtcttact
     3361 gctctccatg cttgtttttg cgccagtgat gtcacagatt ccaatgccag cacttgcagg
     3421 tgttttgatt gtgacggctt ggcgtatgaa tgaatgggaa acgatcaagg agctatttac
     3481 taataaatat tggtcagcag ttctattgtt tatccttacg atgatttgta cagttatctt
     3541 tgatttaagt attgctatcg tgatcggggt gatcggcggc tgtgtatttt ttgttgtcaa
     3601 aagcgctgct atcacgattt cattagaaac gatcgattgg caaagaatgg ggctgccaga
     3661 aacggaaaag ctgaataact ggacagtagt gtatattagt ggtccactgt tttttatgtc
     3721 ttcagaaaaa ctcaaaaata aattggaaga actaaatcat cacgatggtg tcattttctc
     3781 catgcgaggc gtaccaagta ttgatttgac ggcacgtgca gtttttgatg aatttcaaga
     3841 aaaagcagag caaagaggac aaacgattat ctatacttcc ttgcagccag aagtagaaaa
     3901 acaactttct cacttgtggg aacatacatt cacgcatcaa catcaaacag ttgctcatgc
     3961 gttgacagct ttacatgcga aatatactgc aattagccat taaaacaatg aaaaagaaca
     4021 gacaagactg taaactgaag attttatggt cttgtctgtt ctttttaata ttgctgtaaa
     4081 agtgaagcta gtacttgcca gtcttgatgg ataacaggta caagggagcg attgtagaaa
     4141 atagctgctg gatgaaaaag tggaagaatt cgatagtttt tttctgacca ttcgtaggca
     4201 tccttttgtt ctgttaattg taagacaggc cctgtataga gttgaccatg atttttggta
     4261 accgtatatt ctttgccaag taaacgttga agaccaatgt ttccaagtgt cgcaattaat
     4321 tttggctgga ccttcatcag ttcgtaatcc aatattggtg catgcgcaaa tacttcggaa
     4381 cgagtaggtg tccgatttgg ataaacagtt tctgactgtt gtgtccgttt attgatccga
     4441 tgagtcacgc gatacgggcg gctccttaca gcactggtga tatacacctc ttctcgtgtt
     4501 aagccagttg aagaaagtgc ctgcatcaat tcttttccag cttgaccgct aaaaggaaca
     4561 tgcgtatcaa tctctgtttt ccctggggct tctcccacga gcatcagttt aggatgaaga
     4621 ggtccttgcc cagcgataaa tccttctaca ggacggccgt tcattcttaa agaagcttct
     4681 tgtatcagtt cttttgggta ttccatttat gctcctcctt tttcttttat tataagaata
     4741 aaagaaacac aatgcgacta ttttatttgg tagaactgtg aggatagaaa agcagaagta
     4801 ttgcttgaat ttttgtttta ctttttgatt ttaaatttag ttgactaacg gttgctgttc
     4861 tggtaagcta gtactgttgt aattgtggac tccacagcta caaccgctca gaacaagtaa
     4921 taagcacagc atatgctgtc acttcggatg gcgagtctaa gtatatcaga aggaggtgct
     4981 ttatagcatg tacgcaatta ttaaaactgg tggtaaacaa gtaaaagttg aagtgggaca
     5041 agcaatctat gttgaaaaac ttgacgttga agcaggagaa aaagttgttt ttgacgaagt
     5101 tatcttagta ggcggagaat ctacaaaagt aggcgctcca actgttgcag gtgcaactgt
     5161 tgaaggtact gttgaaaaac acggcaaaca aaagaaagtc gttactttca aatacaaacc
     5221 taaaaaacat tcacaccgta aacaaggtca tcgtcaacca tatacaaaag tggttatcga
     5281 cgcgatcaac gcttaatagc gtttgagaaa gaaggcctat atatgatcaa aggaacattt
     5341 aagcgaaatg acgcaggtca gatcgtttca ttcactttga ccgggcatgc tgatgctggt
     5401 ccttatggaa gtgatatcgt ttgtgcgggt gtttctgcgt tagcgatcag cactgtcaac
     5461 gggattgcct cattagccgg ttttgagccg attgttgaaa tgaatgaaga agaaggcggt
     5521 tatctttata ccgaagtcac ttcaggaatg acacaagaac aaaacaatat tgctgagatt
     5581 ctattagaaa atcttttatt aggattacaa tcgatagaag ctgaaaactc agaatatatc
     5641 caaattaaaa caattaatga taaataggag gtgcagacca tgttgttatc tatgaatcta
     5701 caattatttg cccacaaaaa aggtggcggt tctacatcta acggccgtga ctcagaatca
     5761 aaacgtctag gtgctaaacg tgctgatgga caaacagtta caggtggatc aattctttac
     5821 cgtcaacgcg gtacaaaaat ctatccaggt gtaaacgtgg gtatcggtgg agacgacact
     5881 ttatttgcaa aagttgatgg cgtagtacgt ttcgaacgta aaggccgtaa caaaaaacaa
     5941 gtgtctgttt atccagtagc tcaagaagct taataaataa actgctctat ctcttgtgta
     6001 gcaagggatg gagctttttt gtaaattatg ggattttttt tgaccatact tttgaccata
     6061 ctttcttaga agttaatata atttgttagt ttttgtgttg tttcttcttt tttgctgtta
     6121 gtaatgtgtg tatatgtgtc catagtagtt tttatatttg cgtggcccat tctttcttgg
     6181 acttctttgt gatctgcgcc tgcctcatat agcattgatg catgtgtatg tctaaaacca
     6241 tgcagaccga tataaggtaa gccggcacgc tcacgatata tattgtatct ttctgccact
     6301 gactgattaa ttgtgaaatt attttcgtca ttggtaaaca aataattagc tttagtaaat
     6361 cctaattgaa ataagtactt tctttgttcg agcttccaaa attttagttg ctttatagtt
     6421 ttttcatcta aataaattat tcgatttgat tttttggttt taggagtttc agatatataa
     6481 tatttatctg aacgagctac agttttttta atggccattt catttgtttt aaaattaata
     6541 ttgtcccaag tgagtgctaa tatttcgccg attctacatc cgctgaatgc taatagtcta
     6601 aagagcgtat agtctctgtt tttaaaatat ggattctgtt cttgagatac ttcatttaga
     6661 aataattcca actgttcttt tgtatataat tttaatttct tttctgtttt aatttgtggc
     6721 tttggaataa gtaaatttaa tgttggatta tcagatgtta aaccaatatt tatagcatac
     6781 ttaaaaactt tattcatata gtttatgaaa agagggtact gctttgaagt tcctttttca
     6841 ctccaatcat ttactacctt ttgacaatat gccgtattaa ttttagaaag cttaatattt
     6901 ccaaatttgg gatatatatg tttattaaag attattttag tccttgaata tgaactttct
     6961 tttacagttt ttttgtagct ttctagccat aattctgctg cttctttgaa agtagtattt
     7021 gtacttgtgg gcattcctgt tttttgtaat tctagttcta acctagaaag tgctgttttt
     7081 gcttcttttt gtgttctaaa tcctcgttta gtagtatatt ttttctttcc agttagcgga
     7141 tcaataccga gataagtttt aaagtaccat gctttttcgc catttttctt tttatattgt
     7201 tttatcattg ccatcaatat tacccctatt caaattctaa ttctgccatg ctatcattga
     7261 aaaattcgat cagtccttgt tcctctataa attcaagcca tatttccttc ccgttaatta
     7321 atggaactcc tttttcttta cgaattgttt gatacaagtt agtcacgact tgtttccaca
     7381 tttctaatac agctttatct attttcttat catcccagcg ttttttttgg ggtctatttt
     7441 tgtttttttc aatttgataa ttagtagctt tgatgataaa tgtttcgtat ccggttgtgc
     7501 ttttttctaa ccattctttg aattctgtat atatcatttt tgttcacctt tctatttttg
     7561 aacgtatgtt cttttttgct taaaaaattt tagagagctg cataacaata gatgcgagct
     7621 tttaataatt ctttcacaag tggttcgaag cgaacgctta aattgttatc ttctataaat
     7681 ttcatgtaat tgatattgtg tggttctaat tcagactttg caatatatct atctaataat
     7741 ttttcaatca tgaagcgatc agcctcatat tccatttttg aatggagaga gaatgctaag
     7801 ttatataaaa aataattatc atgatgttta gaagcgtgtc ctaattcatg taataaggtt
     7861 ctttgtcttc tatacttaga taaattagct tttattacta tggtattcat acaagcaaca
     7921 taatggccat cggcatcaag gttttcacgc tcttctacct taacgccgag ttcattaatt
     7981 atcatttcaa tttgactgtc caaatgactc acctacttat ttagttatct tttttctcta
     8041 aatatgcttc aatcatagct gatagtattt ccctatcatt atcggtgagc ggttttccgt
     8101 cgctactcat aacagatgct aaagcttctt ccacggttag ttttctttcc tctggtgcaa
     8161 ctcccgcgtt aggatcatct gtgcgtccta gcaaataatc tgttgataca tggaagtagt
     8221 cagcaacttt ttgcaaagtt tctattttag gactttgtgt cttccattta taaatagagt
     8281 tttttccaaa tcctattcta ttttctaatt cagaaataga aatacctctt tttttacaga
     8341 gctctttaac cctttcgaaa agcatgtcaa atcaaccttc ctagagcgta cgataataat
     8401 ttatacaaaa agatagaaag gtagttgaca aatctatcca attgtcttat atttaactcg
     8461 taagctaatt taataagcta acaagcaaca agaaatacac ctaataaaat aaacgaattc
     8521 gaggtcggca aactaagaat gttaattttt agggtttttc taggcttatt taa
//
LOCUS       DACCYH010000109         8494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.114, whole genome shotgun sequence.
ACCESSION   DACCYH010000109 DACCYH010000000
VERSION     DACCYH010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.114"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..173)
                     /locus_tag="IVY85_002577"
     CDS             complement(<1..173)
                     /locus_tag="IVY85_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8045542.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IVY85_002578"
     CDS             complement(593..1714)
                     /locus_tag="IVY85_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAP8045543.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /locus_tag="IVY85_002579"
     CDS             2042..3451
                     /locus_tag="IVY85_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP8045544.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IVY85_002580"
     CDS             3448..4176
                     /locus_tag="IVY85_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8045545.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IVY85_002581"
     CDS             complement(4304..5215)
                     /locus_tag="IVY85_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8045546.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IVY85_002582"
     CDS             complement(5232..6239)
                     /locus_tag="IVY85_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP8045547.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IVY85_002583"
     CDS             complement(6236..8365)
                     /locus_tag="IVY85_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8045548.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IVY85_002584"
     CDS             complement(8370..8477)
                     /locus_tag="IVY85_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045549.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DACCYH010000110         8485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.115, whole genome shotgun sequence.
ACCESSION   DACCYH010000110 DACCYH010000000
VERSION     DACCYH010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.115"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            104..796
                     /locus_tag="IVY85_002585"
     CDS             104..796
                     /locus_tag="IVY85_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8045550.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
     gene            1224..1616
                     /locus_tag="IVY85_002586"
     CDS             1224..1616
                     /locus_tag="IVY85_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045551.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(1789..1971)
                     /locus_tag="IVY85_002587"
                     /pseudo
     CDS             complement(1789..1971)
                     /locus_tag="IVY85_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            1958..2155
                     /locus_tag="IVY85_002588"
     CDS             1958..2155
                     /locus_tag="IVY85_002588"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045552.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(2198..2773)
                     /locus_tag="IVY85_002589"
     CDS             complement(2198..2773)
                     /locus_tag="IVY85_002589"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP8045553.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(2928..3454)
                     /locus_tag="IVY85_002590"
                     /pseudo
     CDS             complement(2928..3454)
                     /locus_tag="IVY85_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            3911..5422
                     /gene="gadC"
                     /locus_tag="IVY85_002591"
     CDS             3911..5422
                     /gene="gadC"
                     /locus_tag="IVY85_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HAP8045554.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            5435..6835
                     /locus_tag="IVY85_002592"
     CDS             5435..6835
                     /locus_tag="IVY85_002592"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP8045555.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(7000..8435)
                     /locus_tag="IVY85_002593"
                     /pseudo
     CDS             complement(7000..8435)
                     /locus_tag="IVY85_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         2820 a         1351 c         1476 g         2838 t
ORIGIN      
        1 ataatttttt agacaaaaaa cagaaagtgt ctaaagaaag cttgacgaag aaaataattt
       61 ttaataactt atttaaggaa attttttgaa ggagaagata aaagtgagaa aaaagattat
      121 tattaatgct ggagtaatta taggattgat gttagtaatt ttctccggaa agcaatatgc
      181 tgataatatt ttattatggg gaggggaaga taatattgaa aagatcaaca acaatttaac
      241 tatattagac acagctctag aaaataaaga gcaaaaaatt agtattttaa attcacaatt
      301 gtcttcaaac aaccaaactc ttgaacaact aagaactaat attgaagttt ccaaacaaaa
      361 agtggcatct ttagaaaacg acaagaatca attagtatca gagaaaacta atttagaaaa
      421 tcaattgtca gcaaaagact ctgaattgca aggaaaacaa aatgaaatta atgcgaaaat
      481 agacgaaatt aaccaaaaag tagctgaaat aaatcgatta aatagcgatt ggaataatcg
      541 tttacaaaat gaaaagaaca aatttaatga agaacaatct actattaata gtttaaacca
      601 gcaaattaat caaatgttga gtgaaaaaaa taacattcaa aatcaactgc aagctactca
      661 gcaaaagcta gaagaagaaa agtcaaaaaa tagtagtttg aataattaca ttgaaaagct
      721 tcaaaaagct aaaaatgatg tagaggatac cgcaaataaa gcacaacaaa ttgtaaatga
      781 acatacaatc caatagatat agttttaatt taacaaatgg tatgtttgga gataatgaga
      841 tagaatataa atgtcataat attatacaat ctaaacaccg tttccttttc tatttttgat
      901 gacatcagta tatagctact cgaaagagta gtctttttat tattctcgct tattattgat
      961 atgaaaattt cgatttgaca aaaggtaccg aataattttg taatatgaat tattatcaag
     1021 aagtaattat gatgataaca tatggaaact agttagtaat aataacaggt gattaaactt
     1081 ttgaacgact tgtaattcta tattttttct tttaagtaaa aaatataggt gatttttttt
     1141 gacttcatat ttataataaa gacacgtaaa tcaattattt ttcattttac tttaactaat
     1201 aatgtttatt tggagggatt attatgttag atgatagatt gacgtgttct aattgtggta
     1261 cattatttta ctatgaggaa gttagaaata ttgtaaaaca tagaaaaaaa gaaatgccta
     1321 tacgttgtcc aaattgcaat gaagttatta agcgaaagtt gtctcatggt tattttgtca
     1381 gctacgaact atatgaagaa agtaaaataa atctgaaaaa aaaaccaaaa tactataaaa
     1441 atttgaaaac tggagctatt ataactgaaa aagaatttaa aaatttagtg actgaagaag
     1501 aaagcaggtt ttataatttg tctggtaata tttgccctgg atttatggat cagattattg
     1561 aaaatgagtc aattgaatca attgaaaact atgttcctgt tgacttacca aaatagaaaa
     1621 aataatcctg tttttacagt gaattatttt aattttgatt ttcaataaaa atatattaca
     1681 ataataatcg taatagctaa tgaaacaatt tctcaatctt tttaccactc caaacaggga
     1741 gcgtcaataa ttttgtgtaa ataactcgtc cttctgcaaa ataatgggtt actcagtaaa
     1801 cattgaagct aatgtatccg tcacttgttg gaagccttta tggcttctat ttaagaattt
     1861 ttgattgtat gtatcaaaga tggatactag gaaacgctct agtgattctt cattttgaaa
     1921 ctgctctttt ctgcggctgt atttcttgat ttgtttattg aaagattcga ttgtagaacc
     1981 agaaggaatg gagaacactt ttgaacttta caacaaaact actgttgata aattaattcc
     2041 aattttagtt ccaactgatt taaatggtca tgatgtttta cttcgtttta acggattttt
     2101 aggtgaggat tatagatacc taaaaatttc tgatcctcaa cctttaacag aataataaaa
     2161 aacgtgtaat caattatttg attacacgtt ttttatttca atcttcgttg tagtagtggt
     2221 tgcgacatac cgaaaggtaa tcttcattac ctcctactac aatctgttca cctgaataaa
     2281 ctggaatacc atcaacaata cgagcattca ttacagcctt tttattgcaa aaatggcaaa
     2341 tagttttaat ttcctcaatt ttatcagcaa atgtcaataa tgcttcggat cccttgaaga
     2401 gattattttg aaaatcattc ttcaatccat agcaaattac tggtacttcc tcattcatta
     2461 cgataagtga tagatcaata atattctctc tacttaaaaa ttgagactca tcgaccaaaa
     2521 tacaatcaac ttttccgtac tgatcaataa tctccactaa ttctccgtac acatccattt
     2581 cagaagtaat tgcaatagct ggtttcgaaa cacctatacg actctcaact tttccaaatc
     2641 ctgatcgagt atctattgcg cttgtcatta tgataactct cttcccttgt tcttcataat
     2701 tatgagccac tttcagtagc tcaatactct ttccactatt cattgttcca tatctgtaaa
     2761 ataattgtgc cattaaattt attcccttct tttacaattc aattattcct taattccatc
     2821 tgcttttatg cagctgctcg tttattcctt gatatgtcta taacatctac tatccctaaa
     2881 accaatttat aagccattag agatggtatt cctgaacatc tctcatattg ttttttgagt
     2941 ggtatatcat ctgaaaaact gaaggaatca tgaaaatagc gacatactta tcatttagag
     3001 ttctaacttt aaatgcattt acttcttcag tcagcacttt agtcatgttg gacatggttt
     3061 ggggcgtgta gtgatgccca tacattttct caatcaagtc tgcgatttcc gacatggtaa
     3121 taccttttcg gaagaggtga atgacggtct cctctaatgt gtcattcgtc cgtctataag
     3181 caggaacagt ctgttgcttg aactcgccat tgcggtcgcg cggaatctgg agatgaagtt
     3241 ccccatactc ggtcttgacc gtacggtcat aggagccgtt acgagaatta cccgtgttaa
     3301 aaccaatgcg atcgtacttt tcgtaatcga ggaaagccgt taactccgtt ttgagaagcg
     3361 tattgacagc tttttccaaa tggacacgga ataatccatt caaatcgcct ttgttcgcta
     3421 gagtctttag gatttctgta gtaaaatcgt tcatatggaa gtcctctttt ctgtgaatgt
     3481 gttgtggtaa ctctattcta cagaagggac ttcctttttt ctatttacac aaaatatttt
     3541 acactctcaa caaaagtatc ttcgattaat acttgctcaa gttatgtgca gctacgaaaa
     3601 agtaggtacc gaggtacttt ctttttcgca gctttttttg gacttatgag aagtacattc
     3661 gctggaagaa tacgatataa aacgagcttt ttcactaaag atattaatag agtgaaagaa
     3721 acaagctttt gttgtcagtt ttttcacttt tctaaaaatg aaaataaaaa attctggaag
     3781 tggagaagaa ttttgcttgc tatactttgt cttgtagaaa agaggtagac aaaataatgc
     3841 aagctcaaat aagcgatttt ccataacctt ggtaggaatg acaaattaaa taggaaaggg
     3901 aagtaataat atgaataaaa aaaagttatc tctattcggt ttttttgccc tgacagcttc
     3961 catggttttg actgtttatg agtatccgac ctttgcaaca tcacaattac atttggtttt
     4021 ctttcttctt ttgggaggta ttttatggtt tctaccagtt gctttatgtg ctgcagaaat
     4081 ggcaacagtt gaaggatgga aagatggtgg gattttcagt tgggttagtc aaactttagg
     4141 agaacgtttt ggttttgcag ctattttttt ccaatggttt caaattacag taggttttat
     4201 tacaatgatc tacttcatac taggagcgct gtcgtatgta attaattttc cagagcttaa
     4261 tacagatcca tttatcaagt ttattggtgt attagtcatt ttctgggtac tgactttttc
     4321 acagttgggc gggaccaatc gtaccgctaa aattgctaaa gcaggatttg tcattggaat
     4381 tgttattcct tcgatcattc tctttggttt agcagcagcc tactttatcg gcggtaatcc
     4441 gattcaaatg gctattaatg cgaaagcttt tattcctgac ttttctcagc tttcaacttt
     4501 agtagtattt gtatctttta tattggctta tatgggcgta gaagcttcag catcacatat
     4561 taatgagctg gaaaatcccc aaagaaacta cccattggca atgtttttac tagtgatttt
     4621 ggccatcgcc ttagatgcaa tcggcggttt ctcagtagca gcggttattc cacaacatga
     4681 cttgtctctc agtgctggtg taatccaaac atttgaaacg cttattttac attttaacag
     4741 tcatttacat tggttggtaa aagtgattgc tctcatgatt gcctgtggcg ttatgggtga
     4801 agttagttct tgggtagttg gtccatcacg agggatgttt gcagctgctc agcgaggtct
     4861 gttaccagtt ttcttccgga aaactaataa acaagacgtt ccagttccgt tgattatgat
     4921 acaaggaatt atcgttacta tttgggcagc agtcttaact tttggtggag gaggcaacaa
     4981 tttatcattc ttagtagcaa tctccttaac tgttgtcatt tatctcgtag gatatttact
     5041 atttttccta ggttactttg tattagtttt taagaaaaaa gacttaaaac gaacttacaa
     5101 tgtaccagga aaaactgttg gcaaaacgat tatcggtgga attggtttta ttttatcagt
     5161 gttcgcattg cttatctctt ttgtaccacc agcttcaatt gcagctagtg agcaccgaac
     5221 ataccaaatc atactactta ttagctttgt agtgactgtt cttttacctt ttatcattta
     5281 tgaagtacat gataaaagtg ctcacacaac tattgaggaa cctactcacg tgaaagctga
     5341 agacgttaat cccgctgttt atccagctgc tagaggagag caccacgtta caaaaaatga
     5401 tgaacatatt ttaaaacatt aggaggaatt cattatgtta tacggaaaag ataatcaaga
     5461 agaaaaaaac tatttggaac caatttttgg ctctgcaagt gaggatgttg acttgcctaa
     5521 atataagtta aacaaagaat ccattgaacc acgaattgct tatcaattag tacaagacga
     5581 gatgttggat gaaggaaatg cgcgattaaa cttagctact ttttgtcaaa cgtatatgga
     5641 acctgaagca gtgaaattga tgacccaaac gttagaaaaa aatgcaattg ataaatcaga
     5701 atacccacga acaacggaaa ttgaaaaccg ctgtgtaaat atgattgctg atttatggca
     5761 tgctccaaat aatgaaaaat tcatgggaac ttcaacgatc ggctcttcag aagcctgcat
     5821 gctgggtggt atggccatga aatttgcttg gcgtaaacgt gctgaaaaat taggtcttga
     5881 tattcaagca aaaaaaccta acctggtgat ctcttctggt taccaagttt gttgggaaaa
     5941 attctgtgta tattgggatg tggaactgag agaagtccca atggatgaaa aacatatgtc
     6001 aattaatcta gatactgtca tggattatgt ggatgagtac acaattggta ttgtaggtat
     6061 tatgggtatt acttacactg gtcgttatga tgatatcaag ggtctgaatg atttagttga
     6121 agctcacaat aaacaaactg actataaagt atacattcat gttgacgctg catcgggtgg
     6181 cttttatgca ccatttactg aacctgatct agtttgggat tttcaattga aaaatgttat
     6241 ctcaattaat tcttcaggtc acaaatatgg tttggtatat ccaggtgtgg gttgggtctt
     6301 atggcgtgac caacaatact taccagaaga attagtattt aaagttagtt acttaggtgg
     6361 agaaatgcca actatggcta tcaacttctc tcatagtgca gcacaactaa ttgggcaata
     6421 ctacaacttt gtacgctatg gctttgatgg ttatcgtgat attcaccaaa gaactcatga
     6481 tgttgctgtt tatttagcca aagagatcga aaaaactggt atttttgaaa tcattaatga
     6541 tggatcagaa ttgccagttg tgtgctataa gctgaaagaa gatcccaatc gcgaatggac
     6601 actatatgat ttatctgatc gtctgttaat gaagggatgg caagtcccag cctacccatt
     6661 acctaaagac ttggatcaat taattattca acgcttagtt gttcgagcag actttggaat
     6721 gaacatggct ggtgattatg tacaagatat gaaccaagca attgaagagt tgaataaagc
     6781 tcatattgtt tatcataaaa aacaggatgt gaaaacgtac ggctttactc actaaaattc
     6841 ttcagagaaa tataaaagaa gttaattaac taataaaagg agttgtgatt accacacagc
     6901 tccttaaatc tatttgaata aatatatgat attcatttgt tacactctca atcttagctg
     6961 ttgttctatt catcattact cttattgtaa gtagttcagt caaatttgct tgttcaattg
     7021 atcgataaca gtatgaattg tttgaaaagt aatcggatat ataattgttt tctcactacc
     7081 taagccttct tggtataaag aactatcgca aagtataatc cgctgagtac aaaagaaagt
     7141 gtcgtctact atttctctaa gttgtttaca aatgatatta ctaatagaaa agtgttctcc
     7201 caaaatgtct tctaactgtt gtcgatagta taaataccta aagtctgacg gagctactat
     7261 gaacacttca attggagatt tccgtttatg ctcatttaaa agacttaaac taatggttag
     7321 tttacgaata gaattctcat tccaacgcag aaataaattg ttttgttttc gccattgttc
     7381 aagaatttca tgaacttcta agtacaaagc gcgttgctct tcatttaaca agtaagtctt
     7441 atcaggttga aatatctgaa tatcagccca aagtgagcgg ataaacggga gaagtgcttt
     7501 atcaaaaagt atctgatttt cttcagatat attcagagat aagataagtt tttgactgag
     7561 ctcaatcacc aaagggttat ctgctacatg gttttgataa acaacgttca agtctttttg
     7621 taacaattca aagttatcgc atagataatt tctagagttg aatagaaagt agatataaag
     7681 gcattcatct tcgtcatgac ataaagagaa agaaagttgc tctaatcctt tctgtattag
     7741 ctgaaacaag ggagtatgtc gggcaactct tttttcttcc tgactaaaat atattgggtt
     7801 agtcttagat ctctgcaaac cgatagcgat acctaataaa ataaattgtt tttcaactgt
     7861 agggtaactt cttgaaaaaa atgtttttct acataatcaa tcaattgttg accgctttga
     7921 ataattgatt tatttagctg gatcaactct gtccgatttg tctcatagat tatcgttagt
     7981 aacaaacttc gataatcctt ttcaggaatt tcaaaatggc catcagcatt taagtactgc
     8041 atttgtttaa aaaaattaat caactcattt cgtaagttgt aaactttgct taacgaaatg
     8101 aaggtagtat cagctatatt actccaattg tgttcccctg ataaataatg tcctagtaca
     8161 ttgaaaaagt cagagcgctg atagatgagt tgagtgagtg ccaacctcga aaacgaagag
     8221 tcgaatgcga ctgtaaagct acttctagat ttctccactg aaataatttg ctgatggagt
     8281 atgtcggtaa tgcattccaa gtcgttagtg acagtgagtg ggcaaacgtt caattgatct
     8341 gctatttcgc gaatctgaat ggtccctttt tcgtgtaatt gctcacaaat gtaaacttga
     8401 cgaaaaatac ttttctccag ataggtttcc aacatagtac tcctcctagt cattttatac
     8461 ataactatta taactctacc aatcg
//
LOCUS       DACCYH010000111         8099 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.116, whole genome shotgun sequence.
ACCESSION   DACCYH010000111 DACCYH010000000
VERSION     DACCYH010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.116"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..566
                     /locus_tag="IVY85_002594"
     CDS             <1..566
                     /locus_tag="IVY85_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8045556.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
     gene            967..1572
                     /locus_tag="IVY85_002595"
     CDS             967..1572
                     /locus_tag="IVY85_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP8045557.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1588..2160
                     /locus_tag="IVY85_002596"
     CDS             1588..2160
                     /locus_tag="IVY85_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8045558.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2412..2669)
                     /locus_tag="IVY85_002597"
     CDS             complement(2412..2669)
                     /locus_tag="IVY85_002597"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045559.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2795..3610)
                     /locus_tag="IVY85_002598"
     CDS             complement(2795..3610)
                     /locus_tag="IVY85_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045560.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            4127..5452
                     /locus_tag="IVY85_002599"
     CDS             4127..5452
                     /locus_tag="IVY85_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP8045561.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5445..5795
                     /locus_tag="IVY85_002600"
     CDS             5445..5795
                     /locus_tag="IVY85_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045562.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            complement(6866..7141)
                     /locus_tag="IVY85_002601"
     CDS             complement(6866..7141)
                     /locus_tag="IVY85_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP8045563.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(7113..8066)
                     /locus_tag="IVY85_002602"
     CDS             complement(7113..8066)
                     /locus_tag="IVY85_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8045564.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
BASE COUNT         2732 a         1279 c         1459 g         2629 t
ORIGIN      
        1 attattttaa gtctgaaaag gatacatcgt ctctaacgtt aactttagag gaaggagttg
       61 aacctgacaa ggtgacctct aaagtgataa accaattgaa agaaaaaggt agtttgaaac
      121 atttggggga atatgaagtg ttagatacgg ctatgttaac gaaaggaatt ggtcaaatcc
      181 tcagtacgat cacttatttt ataacagctg tagcaggtat atctttattt atcgcagggg
      241 taggtgtaat gaacatgatg tatatatctg tatctgaacg aacaaaagag atcggtataa
      301 gaagagccct aggagctact cgaaaatcga ttatgctgca gttcctttta gaaggtttga
      361 ttttaactct ttcaggaggg ttaattggct atttattagg aatggctctt gcttatagtg
      421 ttggttcttt gataaaggta aacgtgtcac ttgatctatt tactatttta ctagcggttg
      481 gggtatcttc ggttattgga ctggtctttt ctgtaatgcc cgcttcagag gctgcaaaaa
      541 aagatttaat tgatattctt agataaaatc agaatgacaa gaatatttac gttctagatt
      601 ctaaaataaa acacaaagag accaaccagg caccctattg tttctttgta gtatagagat
      661 attctttggc ttgtaagaga tcaatagagt taggtgaccg gagttcgaaa taaaggaaat
      721 ttttgattgt ataggagaat tctttatccc taatatttag aattttgagt atgccatgtt
      781 gatgttttag attctctata aatatagagg tgtatcaaag gtcaactaga ctgtgcaaaa
      841 tagagacact tgaaaaatct atcgggggtc agaaacttaa tagtttttga cacataaaca
      901 aaatcgcgat ataatggggt tgagagtgtt tcataaactt atgtcataaa gttaaggaga
      961 tttcgcatga ttttagagaa taaattggga ttaaccaatc aagttgagtt ggctaaggtt
     1021 gaagaaaaac tcagtaaaca aaaagcgaaa gaattgtacg attgtggaaa aataaatgaa
     1081 atcgaagttg gtacatttaa aggtttatct gaaattcatg aatttctatt ttcggatatt
     1141 tacgattttg ccggaaaaat ccgatcggtc aatattgcaa aaggaaattt tcgatttgca
     1201 ccagttatgt atttagaaca ttcgttacag catattgatc agatgccaca aacttctttt
     1261 gaagagatta tcaagaaata tgtggagatg aatatcgctc acccatttcg agaaggaaat
     1321 ggccgaagta ctagaatttg gctggatctc attttaaagg aagaattgca aaaagttgta
     1381 gattggaact taatcgataa agcagactat ctttctgcaa tggagcgaag tcccataaac
     1441 gatttggaaa tacgctatct aatttccaat gctctgactg ataaaattga cgatcgcgag
     1501 ttatatatga aaggaatcga tgtcagctat ttctatgaag ggtattctga gtatacgata
     1561 gatgatttgt aaaagtaggt ggaccaaatg acgaaatatg gatatgcacg tgtctcaacc
     1621 gcttctcaaa gtacaaaaga acaaattcaa cagttgcttg agaacggcgt agagaagaag
     1681 aatatttttt cagaaaaatt tacgggtaca aaacgggatc ggccaaaatt caatcagctg
     1741 ttggctggtc tgaagcctgg agatgaaata gttgttacga agcttgatcg atttgctcgt
     1801 aacacgagag aagcattaga gatcattgaa cctttactcg atgataacgt aacgatcaag
     1861 gttttgaatc taggaacgat tgaaaatacg tccatggggc gaatggtgac acggacgttg
     1921 ttgagtgtag cagaaatgga acgtgatatg attgtggaac gtacccaaga aggaaaaatg
     1981 tttgcaagaa agaataatcc taatttcaga gaaggacgtc cgaaagcaac aatcacaccg
     2041 aagaagaggc acgcatatga actgcttact tcaggtaaat cgtataagga agttgaagct
     2101 ataaccggtt tcagtcgcag tacgcttttt agaataaaga agaaaattga agaaagttaa
     2161 aaaagaagtc tcagcgaacc agacaggtaa atacttaatg cgttctagat atgagtctat
     2221 tagcatgttg tgaaatataa tattttttta tttacttgat ttctgctcaa aaaatgtgta
     2281 tggtaactgt aaaaggtaaa gtaattaaag gtatgctagc cacgcttact atattaggat
     2341 accatcatat atttataatg aacgatttgt tcgtctacca aacaacacat atcgttcagc
     2401 gtaagtcacg tttaggaacg tggctttttt agtgttatta tcgataaata atctattgac
     2461 tctattgatt ttatctccat atccctacgg tgttgattgt ctttagatct ttcatttaag
     2521 aaccaaataa ctatcataaa aatcagcact gctgctaata taataaacag cattcctatt
     2581 gtgatattac ctattgattt taccataact acgattaaag taataatggc attcaagtct
     2641 cctttgttct ctatatataa attagccata aatcatcttc ctttcaaatt aagatacttg
     2701 tttgatacca ataaatagta agcgtggcta gcataccttt ttctgtaata gcaagtgttc
     2761 taatagtcca aacttaatga tggaccgatg ctagttatag tacagtaatg ttttctttct
     2821 tatctagatt tttagtaagc tctatcagca aatctaacaa atctgagtcc ttttcagaaa
     2881 ctaaattaaa tctagtgttt acaacaaagt tttcatgttc tatattgttt agattatttg
     2941 cggaaaggag ggcttctaga tcttgacatt caaatctata tgcatcgtca taaatcttac
     3001 tagaaggagt gcttaattcg aaattactta ttttctcatc ttggaccaag ataaatttat
     3061 agatagtatc tttttgtgtt tttcttttaa aaacacaaca gagtgtgtta taaggaacgt
     3121 tataattttc atcagcctca ttttttctcc attcaaacaa atgataaact gcaaccaagt
     3181 tgccatcaat ttcatggaca gtgctctcgg taacatcctt taaagaacta tcaagggcta
     3241 atttatagta actagagaga ggaagtaaaa ttgtcgattt actgagatgg ttatcgtcta
     3301 tacgtgcaat catacctaaa tcaactaagg ttttattcca ggtgtttata gttttagttg
     3361 tcactcctag agactctgct atggtattga ccgaatgcca ggaatccccg ccttcattct
     3421 ttgccttaaa acagtaatac agatataaat taatagcttt gttattcggt aaatattttt
     3481 caagttctgt tggtagaata aagtaagaat ccttaatgtt ttttctgaac tgtctccagt
     3541 ctctgtagtt ttgtttataa agttgtgaaa cggtttttcc acctttagaa agtcctgcta
     3601 tcttaaccaa tcttattgct cctttcttga atctgtcatg atcatacagt attttttact
     3661 tgtagtccag caccttaata tattatccta tgtaattaat ttcgtactag atagtaatta
     3721 ttttttattt aattctctag tcacttacaa ataagttata taaactacga ttaataaatt
     3781 attacaaaaa ctattaagaa aacttctaca tacaactatt acaaaactat taagaaactg
     3841 aatactccta ttttttgttt ttaaggaaag ttttttcttt tttgaatttc ttattttttt
     3901 ctaatataac taagcaaaac tggataatac gacacttatt ttcaaatttt cacaagacta
     3961 atagtaaata tccatttaat aaagtgatac aatgtgttga ttttacaaat caacagaaga
     4021 agtagggaaa taacttacct atgcttcttt ttttgtacat ttaatcgatt cttctttaca
     4081 aaaacgaacg tatgtttgta taatgtggaa gaatggagtg ttatttatga tggattattc
     4141 aaaagagcca gtaaatgatt actttttgat cgatatgaaa agcttttacg caagtgtcga
     4201 atgtatagaa aggaatttag atccattaac gactgaatta gtcgtcatga gtcgagcgga
     4261 taatacagga tcagggttga ttttagcttc ttcaccagct gcaaagaaga agtacggtat
     4321 ttctaatgtc agtcgaccac gagatctacc gcaacctttt cctaaaacgt tacatgtcgt
     4381 tccaccacgc atgaatctct atattaagcg aaatatgcag gtaaacaata ttttcagaag
     4441 gtatgtagca gacgaagatt tattgattta ctcgatcgat gaatcgatct taaaagtgac
     4501 cagatcaatg aatcttttta cgactgaagg aacaagaagt caacggagaa agaagctggc
     4561 tcaaatgatc caggagaata ttaaagaaga gctaggattg cttgctacag taggtgtcgg
     4621 agataatccc ttattagcta aacttgcttt agacaatgaa gcaaaacata atgatggatt
     4681 ccttgctgaa tggacgtatc aaaatgttcc tgataaagta tggaatattc ctgaaatgac
     4741 agacctttgg ggtattggat ctcgaatgaa aaaaagactc aaccaaattg gtatcatgag
     4801 tattaaagac ttagctaatt ggaatccata cactattaag agccatttag gtgtgattgg
     4861 cttacagctg tattttcatg cgaacgggat cgatcgaaca gatatagcga tcccaccaga
     4921 accgacaaag gaaaaatcat atggtaattc acaagtgtta ccacgagatt ataccaggag
     4981 aaatgaaatc gaactggtgg taaaagaaat ggcggaacaa gtagctattc gtatcaggca
     5041 acataactgt aaaacaggtt gtgtccattt atacattggc acgtcgatct tagaaacgag
     5101 accaggtttt tcacaccaga tgaagatccc tataacagat aatacaaagg aattgcagaa
     5161 ttactgtctt ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc acgttggaat
     5221 aacctatagc aagcttgtgt acactgattc gcttcagctg gatttattta gtgatccaca
     5281 aaagcaaatt gacgaagaaa atttagataa aattattgac aagattcggc aaaaatatgg
     5341 ttttacgtcg attgtacatg cttcttctat gttagaaggc gctcgtagta ttactagaag
     5401 tacattggtt ggtggccatg caggcggcgc aggagggctt aaaaatgatt gattataatg
     5461 atcgtggacg tttaaagtgg gcaggttttt atttatctga tcatactgaa aaaattgatg
     5521 ccgacaatga acaacgagcc aatcaaaatt tggcaaaaga acaaatgacg actgaagagg
     5581 ttgcggaagt attgaacgct gcagtcataa aaaatagatc agtatgtatt caaaaagaag
     5641 aacgaaacgc tgaaggatac tatccagaag atattgttag caagatctta ggtagtgatg
     5701 agctggggat ctacgttgga aaaaacaaag ttcactacga cgaaatacgt catatcgaat
     5761 tttaccagga taataaatgg ggtcgattag aatgaacatt atccagcaat atgagctaaa
     5821 gtatataacc tttgatcagc tatcagaaga gatttggggt tatggccagc gtttgataaa
     5881 tgaggttggt gttgaacgct tctctttcta tgtagaagct gcagcaggat accataaata
     5941 cgaatactat attggctatt aaagtgcaat aaaattatat ttcttttatg ttttacattt
     6001 tgtatcaatt ctgataaaat tacctatagg taattaaaaa atataaaaaa actagttaca
     6061 accttaggac gttgtaacta gcttgaagca tactagagct aactaaaata gcactagaaa
     6121 gcaatgttaa acccgtccaa caaacttgat ttaacattga gaccagctta ttcagctaaa
     6181 tatagttgaa taagcttctt ttgttttaaa aaaacaaaag gtgtactacg aataacgcaa
     6241 gtaaaagaaa tactattaca accagtaggc tacaagtaag tattactaaa cattgcgtag
     6301 ttagaacctt tgctctattg tctaaagcca caaaattaga gatcagcttt atgatgctaa
     6361 gagcgagctg catccatgcg gacataggtt cttttgacac tattaattct cctttttagg
     6421 ataagggaaa ttgctagtag ttttttcatt agcaccatcc cccttcattt gattgagtct
     6481 agtgctgctg tgatagtttg ctgtttaata tgcttacatt cttattataa cataaatgaa
     6541 acaaacacat ggttagaggt cgtatcaatc aaaatagtgt ctcacaaacg atttattact
     6601 tactcctgct gactaacgga ctgttcttca gtaactgtaa ctatatgttt gttaaaacca
     6661 ccattacctg tagataaaaa ccaatatatt tactatactc actatattaa atatatttag
     6721 tatagtaaaa actagaaaca ataaaatttg tgaatgttgt atacccccaa acaatacaca
     6781 tattttgaag gattaggatt aataaagcta aaaatatagt aagtatacta aatatattta
     6841 ctatatctag tatacttact atattttaat cttttgatcg tctattatat ataatccgtt
     6901 cctcgtcgtt taaagtatca acataggcgt ccattgcaag tttgactatt tcatacatag
     6961 gaatatcttt tgcataacta agatcccgaa tggtttttag tacgggagga tctacttgaa
     7021 ctggttttct ttctttcgca gtgtactttc gttcccctaa ctcgacatta atatcatctt
     7081 taattgaaat atcttcagca ccaattttct tcttatcttt gttcgttggt aataatgcca
     7141 tttagtttaa tctcctttcc aagaggtagg atatgattgg ccattgagtc agcatattta
     7201 ggtgtataat caaatccttc aatatcacca tgttgttcat agtattcaat tctttcttct
     7261 aattctgtaa agatgtctgc aaaaatagcc caccatcttc gatctacata atcatttaat
     7321 tggataccat tctcaccata catttctaac cgatccgagc ctttaacaat gcttttaaaa
     7381 atgtttgatt caccatactt atcaattgtt ttttgatagt ttgcgtgttg agaggaacgt
     7441 cgcactgaga aaagaacagg caaaattcca atgatttgaa gatgagactc ttcaaaattc
     7501 gtgacaatcg gaactaaaac tttattaata aaatcttctg ttccttccat tgcgtaccgt
     7561 tttaattctt gcattgcaat aatataatct gcagcagtta agaatgcacg aaccacatta
     7621 tctgttgatg gtgggcagtc gataaagata taatcgaaat tttttctcaa ttcttctaaa
     7681 ggtttaatga aggccaatga tctcttacga cgatcaggat atttttcgga caagtattca
     7741 ttaagttctt cagatccagg agagccagcc ataatatata aatgtggact tacttgagta
     7801 atacctgaaa cccagtcccc ttttttgatt gctgcagaca tggatcgatc aaagctagta
     7861 atttgatacg ttgatccaat gttggaagta gtatttcttt gttggtcata atcaattagc
     7921 aacacgtctt tttcaaaaag ttgactagaa acaatcgcta ataaatcatt gtctgtagtc
     7981 ttaccaatgc cgccttttct acctgtaagc attacaactt ttcctagttt atctgatgtt
     8041 ttaaatgcag tccgtaagtt ttccatagtt ccctccaatt tcctatacta aatatattt
//
LOCUS       DACCYH010000112         8044 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.117, whole genome shotgun sequence.
ACCESSION   DACCYH010000112 DACCYH010000000
VERSION     DACCYH010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.117"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(12..860)
                     /locus_tag="IVY85_002603"
     CDS             complement(12..860)
                     /locus_tag="IVY85_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8045565.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1084..3024)
                     /gene="thrS"
                     /locus_tag="IVY85_002604"
     CDS             complement(1084..3024)
                     /gene="thrS"
                     /locus_tag="IVY85_002604"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP8045566.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3361..3540)
                     /locus_tag="IVY85_002605"
     CDS             complement(3361..3540)
                     /locus_tag="IVY85_002605"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP8045567.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3587..5410)
                     /gene="cadA"
                     /locus_tag="IVY85_002606"
     CDS             complement(3587..5410)
                     /gene="cadA"
                     /locus_tag="IVY85_002606"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8045568.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5688..7967
                     /locus_tag="IVY85_002607"
     CDS             5688..7967
                     /locus_tag="IVY85_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8045569.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
BASE COUNT         2357 a         1709 c         1456 g         2522 t
ORIGIN      
        1 tagtaaggtt tttatttcga atccataaaa tattcttgga ttgcttctcg tgtttttttc
       61 actttttcgc taagcttttc gtttttagcc atatagaaat aaaaaatgat atcgatcgta
      121 gcaaattggg ccaaaagtga attagttgcc gcacttcgat tcgtgatttc ttttggtcta
      181 gatgtttgta cgagtacatc ggctatcttg ctaagcgggt tatttccaaa ttgcgtcaac
      241 gcaatgattt cgatattcat gcttttcgcc agctcggcta atgcgactac atctgcactt
      301 ctacctgagt ttgaaacaag ccaaagcact ttctttttct cattgctgac aagttgcggg
      361 agcaagacgt ggatatcttt ttcaaaaatg attggcttac cgacacgaga ccatttttgc
      421 agaatatctt ctactactaa agaagaggcg ccaaccccat ataaaaaaac agtctctgcc
      481 gattccaaac actctacgac tttttggatc gtttcttctt ctagaatatc tactgtttct
      541 tttatcgcta cttgtgcatt gttaccaagt ttgtttttga tagagtaaaa ggactcattt
      601 gcttctatat cactaaaacc ttcatcgatt ccagtctgta gttctgttga aatcgtgatt
      661 ttgaagtccg tcagactttg aaaaccgatt tttttggaaa aacgtacaac tgtcggcgca
      721 ctcaacccgg aagcttctgc taattcatta gctgtcattg tagggacctt ctcaatatta
      781 ccgataatgt agttggccaa ctttttttct gctgaagaat aatcttccat catactttca
      841 atacgactaa ttacgctcat aagttcctcc acccttttaa tgatagtcta tgtaagtata
      901 ccatatcaga cccttattca cggtcgtata ctggataaaa aacaaaaaaa caatccgccc
      961 aacagaatat cttatcccat caatcccaca gattctattg agcatctgtg tctttgagga
     1021 atacctgtta tctattgttc ggattgcttt ctatctacta cttttttaca ggaaatttac
     1081 ctattagttt tctctgctgt aatttttgac ttctgcttcc atagcatcaa taaagatatt
     1141 caaatcttct acagctgttt ctttacttcc ataacgacgt acgttcactg ttgcatcttc
     1201 caattccttg tctccaacaa ctaattggta agggatcttt tgtgtttgag aggcacgtat
     1261 cttatagccc attttttcat tacgatcatc aacttctaca cgcaacccct tcatttgcaa
     1321 acgttctttg atttcataag cataatcgcc atgtgcatca acagaaacag ggatgattgt
     1381 tgcttgtata ggtgccaacc atgttggaaa agcgcctttg tatacttctg ttaaataagc
     1441 gacaaaacgt tccattgtag aaacaattcc gcggtggata actactggac gatgcgtatt
     1501 ttccccgtct tctccaacat atgtcaaatc aaagcgctct ggcaataaga agtctaattg
     1561 tatagttgat aatgtttctt ctgtaccaag agctgttttg acttgaacat ctagttttgg
     1621 tccgtaaaag gctgcctcgc cttctgcttc aaagtaatct agttctaatt catccatagc
     1681 ggctttcagc attgcttggg ctttttccca cattgcgtca tcatcaaaat atttatctgt
     1741 attgtttgga tcgcgatagc tcaagcggaa acgataatct gtaatattga agtcagcata
     1801 tactgccacc atcaattcaa gcgtacgttt aaattcatct ttgatttgat caggacgaac
     1861 aaatgtatga ccatcattca acgtcatttc tcgtacacgt tgtaaacctg acaaagcacc
     1921 tgatttttca tagcgatgca tcattcctaa ttcagcgata cgaattggta attcacgata
     1981 agaatggatc gtgtttttgt agaccatcat gtgatgaggg cagttcattg ggcgaagaac
     2041 aagcatttcg ccatctccca tatccattgg cgggaacatg tcttcttgat aatgatccca
     2101 gtgtccagat gttttgtaca actcaacatc tcccattatt ggtgtatata catgttggta
     2161 acctagacta acttctttgt ctacgatata acgttcgatc gtgcgtcgga ttgttgcccc
     2221 ttttggaagc cagaatggca aaccagaacc tacttctggg gaaaccatga acaagtcaag
     2281 ttcttttcct agtttacgat ggtctctttc ttttgcttct tcacggagac ggatatattc
     2341 tttcaaagct ttcttgtcaa aaaaggctgt cccgtataca cgttgcatca tttgattgtt
     2401 tgagtttcca cgccaataag cacctgccac tgataataat ttaaatactt gtatacggcc
     2461 agtggatgga acatgtggtc cacgacacaa atcgatgaat tcgccttgtt gataagcagt
     2521 aatcacttct tcttctggca attcactaat cagttctact ttgtacggat cacttgcaaa
     2581 aatttctaat gcttcctgtt ttgaaagaac acggcgttcg atcggcagat tttctttgac
     2641 gattttcatc atttctgctt cgattgccgg caaatcttct gcagttactt ggttttgtcc
     2701 attatctgta tcgtagtaaa accctgaatc gatagctggt ccaacgccaa agtggatatt
     2761 tggatagagg cgacgcatgg cttgggccat caaatgagct gctgaatgac ggactaagcc
     2821 aagtgcatct tcatggtctg gtgtgatgat ctcaatactt gcatcttcct cgattggacg
     2881 agtcaaatca acgagttttc cgttcacttt accagctaaa gctttttttg ctaagctttt
     2941 tgaaatgctt tcagcaattg ccaatgttgt tgttccagac tcaaattctt tgactgcacc
     3001 atctggaaaa gtaattttta tcataatagt ttcctccttg aaatgttttg attgatcaga
     3061 tcatcaagct gaaaaacaaa aaaatcctag catctcttga aaagagatac taggacgaaa
     3121 agttcgtggt tccacctaat gttcaaacgc aaatgcgttc ctcaagcgtt gtaacggtcc
     3181 gaccgccctt gcttcaacaa gggtttcgaa agtggtcatc ctttggtttt ctcctggaaa
     3241 agtttcagcc tagcttttcc tctcttatcg aagactctca aattcagttg gctttctcat
     3301 caatcaatta tgtgtttatt taaccactaa taaatagaaa aatcaagtgg gaaactaaac
     3361 ttattttttc cattccccat ttacaccgta tgttcctttt ttcatctcat tgatgatgac
     3421 atggatgtgt tcttttggtg cctgcgtgtt tttatgaaca gcttccgtga tatctttcat
     3481 catattttct aattgttccg gactgcgacc ttctactaat tcgacatgta cgaatggcat
     3541 aatgctctct cctctttctg atcgttcttt tttcaactcg attctatcat actatgtttg
     3601 tcttgggtac tgaatcagga aaaatggctg gatctttttt tgctctcaag gcactcaaaa
     3661 tagagaccgt atccacgact tcttgcaaaa cagctccgaa taatgcgggg attagtcccg
     3721 tacttgcaat taacatcaaa aaaatacaaa taaagatgcc aatcagtaca gactgtttcg
     3781 ctattctcat cgtatctctt gaaatcacta ctgcttttgc tacacgcaag agatcatctt
     3841 tcaagatgac cgcatctgca ctttcactag cagctgtaga accatgagcc cccattgcaa
     3901 taccgatatc tgcagcagca agtgctggtg cgtcattcac gccgtctcct accatcgcaa
     3961 caggtctttg atctttaggg atactttcta aaatggtcag tttatcttga ggcaagctac
     4021 gggcatgtac agaatctaat tggacttttt ctgcaatcgt ttgagcaatg gcagatccat
     4081 ccccggtgat catcatcaaa tttttcacat caagttttct tagctccttg attgtctttg
     4141 ctgcttctgg gcgaatctga tccgaaaata ggatcgttcc tgcaaaagat cgatcaattg
     4201 ttacatagat tgctgtattg gacgtttcta tttcttcagc tgaaacaaaa gaggcacgtc
     4261 caatctttac ttcatgatta ttgactactg cttggattcc ttgaccggtc acttctttca
     4321 gttgactaac aggatacagt tggctgtttt tcacgttgtt cactaacgaa cgagctaaga
     4381 tatggctaga ttcctgttct gcactagctg catacagaag tagctcttct ttttttattc
     4441 cattgactgg ctgaatcgcc tctacttcca atatcccttt ggtaagcgtt cctgttttat
     4501 caaaagcaat cgtttttgta cgtgcgagct tttcaatcgt tgttccagtt tttacaacga
     4561 tcccgttttt acttgaacgg ctcattcctg caactaaagc aatgggcgca gctagaatca
     4621 gtggacaagg tgatgccact accagtacct cagcaaatcg taaagggtct tttgagaacc
     4681 accaagaaat accaccgatc aaataagcaa tcaacgtgaa agggactgca taacgatcag
     4741 ctaaacggac aaatcgggca ggcttttctt ttgattcttc gactagttta ataatcattt
     4801 gatactggct gtctgcggca cgtttttcag cgatcatttg gatcgatccg tctccattta
     4861 aagaccctga cataatctga tctccaggtt tcttggtttc cgggcgggac tcacctgtca
     4921 aagaagattc atcgaccgaa gattctcctt tggtcactcg accatctgcg ggtacaagtt
     4981 cgccaggctt gactagtaac tggtcatcaa tatttatttc atcgattact atatcctgca
     5041 attttccttc tttgaatcga tgggcaattc ttggggtgtt atctaacaaa gactgcagtt
     5101 ctttacccgc tcgtttattc gcgtaatcct ctagactatc tccgcccgta agcatgatca
     5161 atatcatcaa actagcccag tattctttga cggctaatgt tgcaaggatt gcggtaatcg
     5221 ctaatacatc tacgccatat cttcctgaac gtaacgtctt gatcatttcc aaaaacatca
     5281 agaaggcgag aatccctcca gtaaatgcga caatcgcaaa tgcaagggcc ggctggtgaa
     5341 aaacaaattc acttaataaa gctatcccac caataagcaa agtaagtaaa aattttttga
     5401 aatgtgtcat tgttttttct cctcttttat gtgacttaga gaggttagta ataaatttcc
     5461 agctgcctct ttcatcttaa gcatagtcct tgactagcac tttttaaaac aataggcctt
     5521 aattgaaaac aactatcact atcagcgttt ccaaaattca tcagttctac aaacaaaaat
     5581 attcagcaag gattactttt tcgattcatc agttaaaaag tacctttttt cgaaaaacta
     5641 tgctatactt ttaattcgtt atttacggcg gaaggagcaa acatgcgatg aatgagaaac
     5701 aactagaaca aacgcacgtg gatgaaacga tcgaactaat ccagatcgaa caaaatatgc
     5761 tggatacaaa acaacaagaa ttgactcagg aaatgaatca ttcgacaaaa gacacagcta
     5821 atcacaagat tcgagctggt tccaatgaag ctttttatga atctgctgtt gaatatcgcc
     5881 aacatgagca ggaattgctt ttgaaatacc atactcttga agcacagcaa aaacgcctgc
     5941 aaacgttgtc tgtcatgaaa gatagtcctt attttgcccg tatcgacttt aaagaagacc
     6001 gtgaaacaga aacattatat ctaggtatcg cttctttaag agatctggca gaagaaacaa
     6061 tcgttattga ctggcgcgca ccaattgcca atctttatta tgaaggtgag attggtcccg
     6121 cattttatga aactgaaaca gatagaatag atgtcgatct gcagttgaaa cgacaattta
     6181 aaatccaaga aggacgtatt ttatctatgg tagatacgtc ggaagtaatc aatgatgact
     6241 ttttgctaga gatattggat gaatcatcaa gcaaccatat gaaaaacatc gtctctacga
     6301 tacaaaaagc acaaaatgca atcattcggg acacaacaag taaagtaatg ctgattgaag
     6361 gaatcgccgg aagtggaaag acatccgctt tgcttcaacg tattgctttt ctgttgtatc
     6421 ataatcgcaa gtggctcgag gctgaaaacg ttcttctatt ttcacccaac catttgtttt
     6481 ctgattacat ctcgactgta ttgccttcat taggtgaaag cggcgtgcct acacagacat
     6541 tcaaaaatta tttggtgcag ctgctgccag agttttcttt actagatgaa cagcaacaag
     6601 aaagtggttt tttgctaggg gaaaaagatc cgatacaagt gatgaaaagc ggattgacct
     6661 tagtcgatca gattgatcgc tatatccaag gcatcacaaa ctacggtccc ttgttccgtg
     6721 atatgaaaat aaatggccgt acgatcctat caaaggaatc gattcgccaa tggtataaag
     6781 aaacaaatga acaattacct ttacatcata gactttctct attacaaacg aagctcctga
     6841 aaaaattagg cggtctgcaa aaagacgaaa cacgtcagca atgggtcaaa gatctggcgg
     6901 aagaacagct ccaagaacta tacgcaacag atccaaatct tgaatataca gaacaaaagg
     6961 agcgttcatt acgtaaaaaa ctggccaacc aaatcgtacg taaaagattc cgtcgaatcc
     7021 atcgagggat catgaattat caatttgtca atataacgaa acaatatctc cactttctac
     7081 aaactgtgcc actttctctt ctaacacgac ataaaatcag cgagaccagg tggcaggaac
     7141 atttgacaac tgttcgtaca cacttgcgtc aaagagaatt gcgtcaagag gatgctgtac
     7201 tattcttttt actgatgcgc cgaacatatc cagtgacggt tacacacaaa gctcgctata
     7261 tctttattga tgaaatgcag gatttcgcac catcacaagt ggcattgctg caagccttgt
     7321 tcccaaaagc aaatttgact ttctgtggag atctaaatca aaatgtcttt ggaaatgaaa
     7381 cgatcgttgg atcattggaa gaccttttcg tggaccgtga agtcactcgt ttccaattaa
     7441 cgacaagcta tcgctccaca aaagaaatca ctgattttgc taatcatttt attgtgaaag
     7501 aacatcaagt agaaacaaca gcaagaaaag gagaccttcc tttgctcatc caaggaacaa
     7561 cagatgatat caaattgaat tggttagcag ataccattcg aacgactcag ggaaaagccc
     7621 ggtattggcg tacagcaatc atagctaaaa cacaagaaga atgcgaagct ctttatgctc
     7681 aactgccaga cgagttgaaa caacaagtcc agctgatttt aaatgagagt gactttatga
     7741 agcgttccat tattttactt ccagcctttt tagcaaaagg tctggagttt gaccgagttt
     7801 tcttgtggga tgtagatgca gaacattttt cttctcagca agacaaattg atcctttaca
     7861 ccatgtgtac acgtgcgatg catgaattga ccctttttac ctccaataat gaaagtcctt
     7921 tgttaaacaa tatggatatt tcatcttatg agaaaaaagt actgtaagta aaaagacaac
     7981 tgctatcatt aatgttagca gttgtctttt tatttacaat aataattgca gaaaaagtaa
     8041 ttca
//
LOCUS       DACCYH010000113         7746 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.119, whole genome shotgun sequence.
ACCESSION   DACCYH010000113 DACCYH010000000
VERSION     DACCYH010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.119"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..107
                     /locus_tag="IVY85_002608"
     CDS             <1..107
                     /locus_tag="IVY85_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HAP8045570.1"
                     /translation="NESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(176..904)
                     /locus_tag="IVY85_002609"
     CDS             complement(176..904)
                     /locus_tag="IVY85_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8045571.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(917..2197)
                     /locus_tag="IVY85_002610"
     CDS             complement(917..2197)
                     /locus_tag="IVY85_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8045572.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2338..2940)
                     /locus_tag="IVY85_002611"
     CDS             complement(2338..2940)
                     /locus_tag="IVY85_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP8045573.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3185..3391
                     /locus_tag="IVY85_002612"
     CDS             3185..3391
                     /locus_tag="IVY85_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045574.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3703..4422
                     /locus_tag="IVY85_002613"
     CDS             3703..4422
                     /locus_tag="IVY85_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8045575.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4503..5186)
                     /locus_tag="IVY85_002614"
     CDS             complement(4503..5186)
                     /locus_tag="IVY85_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP8045576.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5405..7591
                     /locus_tag="IVY85_002615"
     CDS             5405..7591
                     /locus_tag="IVY85_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8045577.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2780 a         1302 c         1464 g         2200 t
ORIGIN      
        1 gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca ttttatccag
       61 atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag gcaaaaaaga
      121 accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt tctttttatg
      181 aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt gccaacgtga
      241 cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca taggatttat
      301 gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca tagaattgta
      361 attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa gtagaaaaat
      421 attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt ttgaagatat
      481 ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata tgctcgacat
      541 tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg atgttgcttt
      601 ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt cctaaaggat
      661 ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct tctagttcga
      721 tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg agaagttctt
      781 ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg attctatttt
      841 tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata taaatgggaa
      901 tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa cgctggtttt
      961 caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc aagataaaca
     1021 agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt ttcaactggt
     1081 ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa tgattcttct
     1141 tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca atcgcattgt
     1201 ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg
     1261 gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg agttttaatg
     1321 acagtaaact acggatctca ggaactttca tattatccaa catacttaga ttggccggaa
     1381 aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac cgtaatcctt
     1441 ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg taatcatgtt
     1501 tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc tgttgcatcc
     1561 tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc caataataaa
     1621 ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc aaagctaaga
     1681 catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat gtaaataata
     1741 tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa gtctctaacg
     1801 atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca atgtgaaaat
     1861 actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa tcgccgatgc
     1921 ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg taaaccaatt
     1981 gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata gcaattagag
     2041 cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa atcagaatga
     2101 ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata caaattgtgt
     2161 aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc cattgatttt
     2221 taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat tttgaattca
     2281 gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt taattgttta
     2341 atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg taataggaag
     2401 caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt aagaagctga
     2461 gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct ttaataatgg
     2521 gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat agcgaagcag
     2581 taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag tcaattgcag
     2641 atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca gacaaacgaa
     2701 ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag aaagaaaggt
     2761 gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga ccgctagttt
     2821 tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca ccatatcttc
     2881 ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt tatctttcat
     2941 ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt cttttgtttc
     3001 ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct tcgttttcca
     3061 agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat gacaagtttg
     3121 tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg atggagggat
     3181 tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta aatctgattt
     3241 atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg tgatgatacg
     3301 tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact ggcagttagt
     3361 tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag agcataaaga
     3421 ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa acagcttttc
     3481 atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg tttatttgaa
     3541 aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg agttactttt
     3601 ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt tatttctata
     3661 aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat aattgtgatc
     3721 attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct tgcccaagat
     3781 ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc gctattttat
     3841 tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa acgctttttg
     3901 gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat ctacgaagat
     3961 agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga agtaaagcag
     4021 tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc acaaaagaaa
     4081 ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag aatcaaaaaa
     4141 agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta ctataaagaa
     4201 ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa aatagtgatc
     4261 gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat cgtacaagaa
     4321 atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat ccgctacact
     4381 acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa ctaagcaaat
     4441 tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt gattcattga
     4501 gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt ctgagtgagg
     4561 gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa cagatgatga
     4621 cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag atagtggaaa
     4681 aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt cgttgctacc
     4741 ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat tttatcaaaa
     4801 gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc gggatgctca
     4861 tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag aacaaacaag
     4921 cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt tagaagaatc
     4981 cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca ttctccgtca
     5041 aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa agcaccatat
     5101 tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag tcctattgta
     5161 cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata tttttgtctt
     5221 ccttatctta gcataaacga ttgaaaaaaa aggaaaaatt ttcagaaaac taaaatcata
     5281 tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat agacaacgaa
     5341 actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga aggaaatcaa
     5401 tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct gggagaacaa
     5461 gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta cctatgaagc
     5521 ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag aaagtgaagg
     5581 aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca atcaagaaca
     5641 aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag gagctgggag
     5701 cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa aacaagtaga
     5761 cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac taaaaaaacg
     5821 agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc atagtatctt
     5881 ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc aaggaataca
     5941 aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac agctgatacg
     6001 taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt ctcggttaga
     6061 ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta aagaaaaata
     6121 tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg gattgctcac
     6181 ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg tactccaaaa
     6241 tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa aatatgatta
     6301 tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg tgaaacggat
     6361 gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga gtatctatgg
     6421 attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc ctaccaaaac
     6481 tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag caaatagatt
     6541 gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac aaaaagaagc
     6601 aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt ggctaatcaa
     6661 acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca tattataccg
     6721 atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata ttccttttgt
     6781 actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc aacatttcta
     6841 tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc aggcctacca
     6901 gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat gcgatcaata
     6961 cttttctcct aaatatcaaa atatagccgt tgcagattat attttgtcga taagccccaa
     7021 tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta aaacagacca
     7081 gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta tagctaagga
     7141 aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg ctactttcag
     7201 aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa aaacccgctt
     7261 gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc acaaaagtaa
     7321 aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt tgccttataa
     7381 cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac cagtaacgac
     7441 gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa atcgtctgta
     7501 tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat ttatcaaaga
     7561 aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca gaagtggtca
     7621 gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaatttttgt gaaatttcag
     7681 cttattttcg aagaagctat ttctggaaca cagtttattc ggaaaaggga ggtgtgacaa
     7741 gaattt
//
LOCUS       DACCYH010000114         7600 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.120, whole genome shotgun sequence.
ACCESSION   DACCYH010000114 DACCYH010000000
VERSION     DACCYH010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.120"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(138..428)
                     /locus_tag="IVY85_002616"
     CDS             complement(138..428)
                     /locus_tag="IVY85_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045578.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="IVY85_002617"
     CDS             complement(546..806)
                     /locus_tag="IVY85_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045579.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="IVY85_002618"
     CDS             complement(943..1356)
                     /locus_tag="IVY85_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP8045580.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="IVY85_002619"
     CDS             complement(1812..2297)
                     /locus_tag="IVY85_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045581.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="IVY85_002620"
     CDS             complement(2436..2651)
                     /locus_tag="IVY85_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP8045582.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="IVY85_002621"
     CDS             2875..3675
                     /locus_tag="IVY85_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8045583.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="IVY85_002622"
     CDS             3694..5562
                     /locus_tag="IVY85_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8045584.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="IVY85_002623"
     CDS             5555..6046
                     /locus_tag="IVY85_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP8045585.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="IVY85_002624"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="IVY85_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP8045586.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="IVY85_002625"
     CDS             6606..>7600
                     /locus_tag="IVY85_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP8045587.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DACCYH010000115         7502 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.121, whole genome shotgun sequence.
ACCESSION   DACCYH010000115 DACCYH010000000
VERSION     DACCYH010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.121"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..281)
                     /locus_tag="IVY85_002626"
     CDS             complement(<1..281)
                     /locus_tag="IVY85_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP8045588.1"
                     /translation="MDLITQYSEIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKY"
     gene            complement(420..1235)
                     /locus_tag="IVY85_002627"
     CDS             complement(420..1235)
                     /locus_tag="IVY85_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAP8045589.1"
                     /translation="MNENKLIKLGVAGVVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKEHGWVTVNGADTVVTDETGK"
     gene            complement(1232..1549)
                     /locus_tag="IVY85_002628"
     CDS             complement(1232..1549)
                     /locus_tag="IVY85_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045590.1"
                     /translation="MEFVDLNVRGIKCDNPECDYSDMAVKYENYPQWLNKPCPKCGAN
                     LLTQEDLDATEQLMEIVNLTNEILKDSGLEKQDMNKYIVPVEANGTGELSFGEIKKLE
                     EEK"
     gene            complement(1549..1839)
                     /locus_tag="IVY85_002629"
     CDS             complement(1549..1839)
                     /locus_tag="IVY85_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045591.1"
                     /translation="MLDMRIEDYRITSDSRNIVLSKVRRDEEGNIRYTEAKEESRADI
                     GYFQTVSSCLKAIQRDYVLSEERTIKSIIEYKKALENITRQFEQACEIEEEK"
     gene            complement(1833..2138)
                     /locus_tag="IVY85_002630"
     CDS             complement(1833..2138)
                     /locus_tag="IVY85_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045592.1"
                     /translation="MDKKATMKRIVELTKSESWQEDKEIVAEVQKLGKSMWTEKPKRK
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEKKC"
     gene            complement(2141..2302)
                     /locus_tag="IVY85_002631"
     CDS             complement(2141..2302)
                     /locus_tag="IVY85_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8045593.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            complement(2317..2676)
                     /locus_tag="IVY85_002632"
     CDS             complement(2317..2676)
                     /locus_tag="IVY85_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP8045594.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            complement(2673..3524)
                     /locus_tag="IVY85_002633"
     CDS             complement(2673..3524)
                     /locus_tag="IVY85_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8045595.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(3541..4371)
                     /locus_tag="IVY85_002634"
     CDS             complement(3541..4371)
                     /locus_tag="IVY85_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8045596.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            complement(4374..5060)
                     /locus_tag="IVY85_002635"
     CDS             complement(4374..5060)
                     /locus_tag="IVY85_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045597.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(5066..5305)
                     /locus_tag="IVY85_002636"
     CDS             complement(5066..5305)
                     /locus_tag="IVY85_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045598.1"
                     /translation="MENELSTLDQYLADPDWGKSNLQLVANKYEKSIDEAIVALTDAA
                     SITADDSKWTKRDLGILKRGVNWLEDQYREETKEK"
     gene            complement(5631..5819)
                     /locus_tag="IVY85_002637"
     CDS             complement(5631..5819)
                     /locus_tag="IVY85_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045599.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            complement(5840..6124)
                     /locus_tag="IVY85_002638"
     CDS             complement(5840..6124)
                     /locus_tag="IVY85_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045600.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            complement(6126..6320)
                     /locus_tag="IVY85_002639"
     CDS             complement(6126..6320)
                     /locus_tag="IVY85_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8045601.1"
                     /translation="MTMSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRR
                     KPDKQIKPIEAALAELEKEK"
     gene            complement(6317..7093)
                     /locus_tag="IVY85_002640"
     CDS             complement(6317..7093)
                     /locus_tag="IVY85_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP8045602.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            complement(7220..7441)
                     /locus_tag="IVY85_002641"
     CDS             complement(7220..7441)
                     /locus_tag="IVY85_002641"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045603.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
BASE COUNT         1853 a         1588 c         1162 g         2899 t
ORIGIN      
        1 ctatactttt ctacaaattt gaaacgatct tgatgaagtt tcttgctcca atttgtttgc
       61 cgatccagct cacgcatctg atcgaaccct ttttgaattt cgttgtaata gaattcaatg
      121 tttgctgctg ctttccaatg cctgctactt cgcactcctg ctcctgtttc agccatttcc
      181 aacttaacta attccgctcg ttcttttgat tttttatctt tctgaatctt catcatgatt
      241 ttcttgagga tgatttcact gtattgtgta atgagatcca ttatttctcc tcctaaaatt
      301 tcatttcatc gtcgttgtca tcttctttgt catcagtttt ggaaaggagg gcatacacga
      361 gatatgccac tcctaccaaa cctaagaaca agagaatttt agctataaag aatcccatat
      421 tatttacctg tttcatccgt aactacagta tctgctccgt ttactgttac ccatccatgc
      481 tcttttcgag cttgagcttc ttcataacga attaaattat ctgttacaga ttcggcaact
      541 ttacggtttg attcagcttc cgcttctgca gcttttgttt tcttgtaagc ttcactatca
      601 gcttgagttt ttgcagtttc tgcatctagc ttcgcttttt cattttcttg accagctcga
      661 atgatcgcat caattgattt ttgtgtttct ttatcgacat ctggaacacc aagtgttacg
      721 tcttcgactt caaacccttt agattcaact gatttagcaa agttcgttag tacctcagct
      781 tcaactttag aggaatctcc tgaaaggaca tcaagcaggc tatatttggc ataaacttca
      841 cgcgctactt tttgaagctt agattttaac catccacttt cgatatcttc cgaagttata
      901 ttcccaaatt ctttgtacat ttttgctgct ttagttgaat caactttgta gtcatatttg
      961 atatcaatcg ttgtcttttt gccatcgctt gttgatactg aaatgttttt tgattggata
     1021 gtttgcaagc gaattggata ttggatcact ttgtcaatcc caacaaattt cacaccttgc
     1081 gtcagtgctt catctttgat accgccattc attgaatagc gcacacccac atatccgtta
     1141 tcaatttttt cgaaaaactt aaatcctccg ataactccaa tacctactat tactactcct
     1201 gccacaccta gtttgattaa tttattttcg ttcatttttc ttcctccagt ttttttattt
     1261 ctccaaatga caattcaccg gttccattcg cttctacagg tacgatatat ttattcatat
     1321 cctgcttctc caatcctgaa tctttgagga tctcgtttgt aagattgaca atctccataa
     1381 gctgctctgt tgcatccaag tcttcttgcg tcagaagatt agcgccgcat ttcggacatg
     1441 gcttgttcaa ccattgcgga taattttcgt attttactgc catatcagaa taatcacatt
     1501 ctggattatc gcatttgatc ccacgtacat ttaagtctac gaattccatt atttctcctc
     1561 ctcaatctca catgcctgtt caaactgtct agtgatgttt tctaacgctt ttttgtactc
     1621 gataatactt tttatcgttc tttcttcact taacacgtaa tcgcgttgta tcgccttcaa
     1681 acacgatgag acagtttgga agtatccgat atctgctcgt gattcttctt ttgcttctgt
     1741 gtagcggata tttccttcct catctcgtct tacctttgat aggacaatgt ttctagaatc
     1801 actggtaatt cgataatctt cgattctcat gtctagcatt ttttctcctc cacataccta
     1861 aactgtcgtc cttttgaatc aatccataag ctcctagctc tatcccagat aatgtttttg
     1921 cttaatccag taatttcaga taactgttca gctgtacctg ttactagaat tcgatcacca
     1981 tgccagattg caatttttct cggcgttttc cgtttaggct tttcagtcca cattgattta
     2041 ccgagctttt ggacttctgc aactatttct ttgtcttctt gccaagattc tgacttggtt
     2101 aattcaacaa ttcgtttcat tgttgctttc ttatccacgt ttattcctcc aatctacgaa
     2161 tttcccttct taaattctct atgtgcaaat cgattgcctt tctcgccgtt tcattgacca
     2221 tcactgcctt tgttcgttct agatcgtcaa tttcacgctg aatgtttcga atacgcattt
     2281 gaatcacttc ttctgttgtc atgatggacc acctcgttaa aaacgctctt ccttgaacgt
     2341 attccgatat tttttagcta atatcaacgg cgcttgatat tgatgacaga acaactttgc
     2401 cttgatctta aagtcttttg tctgcattcc tttgacatct acgactttga caagtttgcc
     2461 gtttttataa aatgtgaagt cgggaatata ctcgatcttg cgatacttct ttccgtctag
     2521 ttcaaatttc ggcatcagct caaatctttc ctgaagtttt actttccagc catttgcttc
     2581 agcttgccac aaggctagat cgtaatactc tgcttctgcg atagaatcaa acttgatacc
     2641 tcgatgaaca gtttttttat tacggtattt attcatgcga tactaccttt cactggtttt
     2701 atgcgcttgt ctgctgtttg ttggaatttc agcgcataac cttctgaatt cttaaatatc
     2761 ctagaaacaa ttctttcacc gtaggcttct cttagttcag gaccagataa gtttgttgtg
     2821 atgatcgttg ccttgttctg tctggcttct aagagcgtgt ttaacgtgtt gtttgtaaac
     2881 tgcctactat ttgatacccc actacctaat tcagctccaa tatcgtcaaa aaccaccaaa
     2941 tcagttgttt taatatcggc tataagcgat ccttcaattt cttttctcag ttcagtattg
     3001 ttataagaaa actttatttg ctctaataat tcttgatagc ttataaaaag tattttcttg
     3061 tcataatttg agcgctcaag tatttcccaa gctgtggcca ttgataagtg gctttttccg
     3121 cttcctgatt tccctgatag aatgaaatgt gcaggatggt tcagcagaac ctcattcgta
     3181 tagctttttg ctttttctaa agcgattttt gtttcttgat ccactacgtg ataattttcc
     3241 attttgcatt taaacaaagt tttatctgtt aataccgaac cattttgaaa aaaactcaac
     3301 gctcgtgctt ttaagctatc gttatatatc cgttcggtct gtatatcctc tttcacacgt
     3361 aacgctttat aaccacaact catgcatgtt ggtttacaac gttctgaacc atccttattt
     3421 ttagctcgcc aactatacaa aggttcgcta cattctggac attttccgct ttgcactaat
     3481 actcttctta ttagtttctc catagcattt gctaggcttt ccatgtgatg catctccttt
     3541 ttaaattggc aagtcgtcat attcactagg attgctgtac tgtagttttt gactttgctt
     3601 tttatgattc ttcttgtctg ctttgatttc gaatttgagc ttctcaaatt tttctctcaa
     3661 tttcttagca cttctaatat ttccaaacca aaattcattt gtaggtagcc aattgatcac
     3721 atactcaatc gcttctatag atgctttatc tctttcttcc atcaacctga ttgtgtctgc
     3781 ccatttttcg atatctactt tgttcatttc ttttggaaaa tcttcagtta aattactttg
     3841 caacttttta gcaaggcgta agtgttcgtc agaatactta cctttctttt cttctttatc
     3901 tatatcttta tcttcttcta tatctttatc tgtaccgtca cgtgacgtca cgctaacgtc
     3961 attttccaat ttgagacgtt cctgtctctt tctttccctg tatttacggt ttctttcagc
     4021 attttttagc cttactttat ccataccctc gatattttga tgtttttccc aattactgat
     4081 ggcaattagt ccatcactgc ttagatcaat catgttgaaa tttgccaatg tagttagcgc
     4141 taagcgaacc gtatttacgt ttttgccaaa caatgtagca agcatttctt cggtataagg
     4201 catgttcctc tggatatata tcagaccatc gtcgttagtc tttcctgcta aaactagtaa
     4261 tcgaatccat ataacgatga tggcatccga ctcaggaaca gcttggatta atcgtatttt
     4321 ttcatcgtca aacatagtag ttttaagttt gatccaactt atctcagcca aatttatcct
     4381 cctatcctta actttttaat tgtttcctgg tttaacttga tccctttgat ttgatattta
     4441 tttttgaaat tgatcacacc tattttgtgt ttctctgtgt gatggattct gcagagtgct
     4501 gcaaatgtgt actctgaatg atcaacttct ttgcgctttc gtcttcccag cgctttgtca
     4561 aagtgatcga tatcagctcc tgttttgcca cagatgcagc agactctttt tgtaatgcat
     4621 ttgtagaagt aatactcttg attcgctggt aaaatctcat atccttcttt gaaaggaata
     4681 tgatgttcaa agatgaaatc taggatgata ttcgctaaga tattggcatc actcacggtt
     4741 gtattcgatt cgtctttgag gcttatttcg cgccctgtga caccttcaaa acggaagtag
     4801 aagaattcct tccagaagtc cgttggcatg cctgtatcga tgaaaatatc gcctatgagc
     4861 gcatagatga agtttctttg ctgtactgtg aaccgtcgtg gatcaataaa gcgaatttca
     4921 atgactcgat cgccatcata tccgtcatac atcgttttca gccgttcgat atttacttct
     4981 tcgttgattg ttgcaccaat gtcgttacct ttaaatttct ttagtaccgc cgagtatgaa
     5041 tctattagtg gtttaaacac tcatatcact tctcttttgt ttcttctctg tactgatctt
     5101 caagccaatt aacgcctcgt tttagaatgc ccaagtctct cttggtccat ttactgtcat
     5161 cagcggttat ggaagctgca tcagtcaatg caacaattgc ttcatcaatt gatttttcgt
     5221 acttgttagc aaccagttgt aaattcgatt tgccccaatc aggatcggcc aaatattgat
     5281 ctagagtgga aagttcattt tccatatgtt aaaatctcct tagttatgat tttttgagtg
     5341 actcattgct ttggtcggcg gagtcacttt tttatttgtt gccatgcttt tttcttttcg
     5401 atatgttgct tgcttaaaat aatagaacgg ctatttgccc accaattatc agcaatcact
     5461 ttaccgattt ttagcgcttc ttctcgtgcc atagttgctc ctttcttttg agtcaagcag
     5521 attgattaaa accatcaatg ctgcgaacaa gcttcctccg ataatacttt ggtgcgctac
     5581 aatcactaat aaccccaaaa tgaatcctat aaaaagtgtg tctgtcttct tcataatcta
     5641 atctccctat tttttatttc tagcattctc aaatcctcaa gttcagaagc aattagttca
     5701 gcttgtctat ctgatagctc atcggatttt ctaagcgctg cacgatcatc ttgtaattgt
     5761 tttctacgct gtttaatcag acctagaatt tgatgttctt gttgcaatgt gtaggacata
     5821 aaatcattct cctttgcctt tagaactcaa agttttcttt caaaaatctt tggagttccg
     5881 atcgttcaat tctgatgtct aacttgctcc actgctgtgt ttttaagcct aggcttatcc
     5941 aatgtgttaa tttgtcatca ccaatgccta aaactttttt tacctctgat ttgtttggat
     6001 atggaggaag ctctactgat ttgttcataa ggtgtaatcg ttcgtccaat gaattaagaa
     6061 cagcattcgt aatctgtgta gttaattcgg aaactactaa attatctgga attgttattt
     6121 gcatgttatt tctccttctc taactctgct agtgctgcct caattggctt gatttgttta
     6181 tctggttttc tacgcccatt cataatgtcc gacatatatg gtctagaaat tccgatagta
     6241 tcagctaacc agttctgaga ctttccgtat tttgctaatt gaattctgac tttcaaaata
     6301 aattcttgtg acatcgtcat tcctcctcta ataaatcgat ttctggaata tatccttctt
     6361 ttttcagcga ctcataaatg aacagacgcc ctttttgagt ccattttgta ttcatcacaa
     6421 cttttgttcc gccatcagat ttcggaatct cagttgtatg agattttgtg taaccctgtc
     6481 tcatgtgttt tttgcataat aaccattggt tgcctacttt tttctgaata cctagcttat
     6541 gaagtagttt gttcatctgt tgtggagaca tcccataatc agccgcgatt tgactaatag
     6601 ttactgaatc tgtagaagat aagatactat ccaaatacga aattttgggt tcgtattctg
     6661 caattttttg ttctgcgatt agtcttccag tacgttcttc tttcaactta gttgctaatt
     6721 ggatgattgt atctggatta agcaaagctt cttctacttt ttctggagtt agataacctc
     6781 catgttttct aattgctggc aacacttcgc tagtcaccca acgtttaaat ttcttagcat
     6841 ttggttgttt gcttcctaaa attaaggagt acaaacctga ttcgttaata atagttaacc
     6901 ctctaggcga ttcaaaagta ccgttttggt agttttgccg atcttctaca tcgacgtgtc
     6961 tgttaatatc tctactacca ttttggtacc ctagaacttt agccacatct tttccaacaa
     7021 aaaatggttc atcatgtatt gttactgttc ttacttcatg ttgttcaaag ttaaaaattt
     7081 gcggtgtgtt catttttgct cattcctttc tttgatataa ttttgaatag aaagcgaggt
     7141 gaaaatagta tggaagaatt taatatggat gtcgacgcct tattcaaaca aaccgtattc
     7201 aagactgtta ataaagaatt caaaatagac ttccaaaatg acgaatcttt tccaacagaa
     7261 attgaattat tcgaagaact ttctcagagc gtttctgagt ctttttctcg tcagttgcaa
     7321 aagaattttt ttgatgctct tgtggatgaa tttcatcaac aacagcacta aacttttggt
     7381 ctttaaaatt taaagttatt aaagctcctt ttgcggaagg agttttttta ttttgtttca
     7441 tgatgtttcc tccttttctt taaatatgta agctaataaa attagctaat ttttattgac
     7501 at
//
LOCUS       DACCYH010000116         7498 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.122, whole genome shotgun sequence.
ACCESSION   DACCYH010000116 DACCYH010000000
VERSION     DACCYH010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.122"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            172..1563
                     /locus_tag="IVY85_002642"
     CDS             172..1563
                     /locus_tag="IVY85_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8045604.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(1636..2283)
                     /locus_tag="IVY85_002643"
     CDS             complement(1636..2283)
                     /locus_tag="IVY85_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8045605.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(2291..2977)
                     /locus_tag="IVY85_002644"
     CDS             complement(2291..2977)
                     /locus_tag="IVY85_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8045606.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(2983..4056)
                     /locus_tag="IVY85_002645"
     CDS             complement(2983..4056)
                     /locus_tag="IVY85_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8045607.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            4278..4952
                     /locus_tag="IVY85_002646"
     CDS             4278..4952
                     /locus_tag="IVY85_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8045608.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            4957..6297
                     /locus_tag="IVY85_002647"
     CDS             4957..6297
                     /locus_tag="IVY85_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8045609.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(6446..7432)
                     /locus_tag="IVY85_002648"
     CDS             complement(6446..7432)
                     /locus_tag="IVY85_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP8045610.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         2328 a         1386 c         1319 g         2465 t
ORIGIN      
        1 tttaatatga taattattat acgaagggcg ctaaagggaa tcacaccaat tttcatacca
       61 attggtttca tttatgaagt tgattccttc gattaatgag cgtttcattg aaagaaaagg
      121 gttgagggag aaaaacatat gatcaggttg ttggaattaa actatagaaa gatggaaaaa
      181 atcattcgaa ttctgtccat ttctgacaag tggatgacta aaaaagaact tgccaaacga
      241 attggtagtt cagaatctac atttgtaagg tatatcgaag agatcaaaat ccgctgggga
      301 acggatgtaa cgattcaatc ttcacaaaaa ttggggtatc gtcttgaaca tttgaatgta
      361 ccagcttatt taaatgttct gatggaaatg tcgcaaagtt ctacaaacag tcgtttatta
      421 aatgagatta ttgtcaatcc gggtaaaacg attgagtatt attgtgaaaa aatttccatc
      481 agccggtctt cttttacacg taagttgaaa caatgcaatc aaattttaga aagctattta
      541 ttaaaagtga tcgttgacca aggttttcag ttaactagcc ttaaaagtga gctccagttg
      601 cgtatctttg ttaccttttt ctttttaagt tactacggaa gacgcacgtt gccatttcca
      661 atcgaaaaaa agaagatcaa agaaattatg gtcaagaata attgtcagat gaatttgttt
      721 caggaagaaa gcccatatga gcaatctttc tttattatgt attttgttgt gcatctcatg
      781 agagaatccc aaggaactcc atttgaatgg aaagctggag actcttatgt acagttgata
      841 aatatcgata aaaaagactt cttaatcttg aaaaatctct taggaaaatt gaagtacagc
      901 agctatataa aagtagtgaa ctattttgtc aatacgattt tttcagaatt ttcggagaaa
      961 gaaaaaaatg aaatcagtga gcagatacga aaaaatatgt cctactgttc gttttcatcc
     1021 gctgattgca aagaaaaaaa cgaagaatat gttgttcaat tattaacgca aatttatttt
     1081 ttctcccgta ttttcccata cgatacgact catttgacga tccgtgcaac ttactttgca
     1141 cagcagatgc aacaccgcca gatacgtcta tatgctggat ttcgtgcaga acttcaattt
     1201 tattccactc tactgaatca gaatctgatt tattatttat ctagctatat cttttggatg
     1261 cttatcggta atcctgaaat tcaccattat acttctgata aaaaaatact gattatcagc
     1321 gacttaagtc ttcgacatag tcaatatatt gaagaatata tcagtgatat tcttgctgtg
     1381 cataaaattc actctgagac aactgctatt actgaagatc agctgtcgaa atacaatctt
     1441 ctcgagtatg atctgatcgt tacgaatcaa cccattttga atgcccatgt tcctcatata
     1501 ttgattgatg atagtgtgtc attcgcaaat gaagaagaac tcatccgctt attcgagtta
     1561 tgaataatca aaaaacagct atgtataaac caccctgata aaaatttcag gacaaataca
     1621 catagctgtt tctttttatt gtattccttc agcaatccaa cgagatttat agcgaaagtc
     1681 ttttaacaac tcttcttttg tccaattatt tgcaatgaga tccgtcaaag tgttcattac
     1741 gagggtcatg gaaaagtata ggaaactaac tgcttcttcc gacgattgaa tggagaaatg
     1801 attgattctt aataattcca gccatttttc gttcattgga gaatcagccg atagttctct
     1861 acgtttggtt gtctgctgat catttcgctg ggttaacaag tataaccttt gccaatagat
     1921 actttcgtga tctaagtcgc agcaccactc taaatatttt tcgatcccca aaaagaaatg
     1981 ttgtttgttt tcgctaatga attttaagat atcttgatgg actatgtgag aactgctttt
     2041 gatcgtatac aaataagcat cttccaaatc ttgaaaatat ttataaaaag caccgcgcga
     2101 catccccatc ttgtcaacga tttctgagac tttgacttgg ctgatgtgct gtgagcaaaa
     2161 tatctccagt aataggtcat cgattctctg tttctttgcg ttcgataaat tgaaaaaagt
     2221 ttcttttggc atccacatct ctcctttatc tctgattata cgtggatagc ccttcattgt
     2281 catgaaggat tcattccttc tttttctctt cttctgtcaa gtacccatct tccattcgat
     2341 aaacgcgatc actccattga atcatacgga catcatgagt aaccataata gtcgcttttt
     2401 gtttctcatg agcttcttta actagtaatt tgacgacttc atatgcatga tctgtatcca
     2461 aactagctgt tggttcgtct gctaagatca agcttggctc attatataaa gcgcgagcaa
     2521 ttgccactct ctgtctttct ccaccagaaa ggtctttagg ataactattt ttaagtgact
     2581 ggatatctaa agattgcagc aaggaatcga tttttgttga atcttctttt tttgaagtaa
     2641 cttggtcaac tagttcaaat tgttccttta ccgttaaaaa aggaatcaaa ttagagcttt
     2701 ggagaataaa tcctatttct ttaaagcgta aatctgctcg ttttttttct ggaagctcag
     2761 ttagtgagtg accgttgatc actatttctc cggaagtcgg tgtttgcaat cctcctgaaa
     2821 tggtaaggaa agtactttta cctgaaccag aaggaccaat gatgctgacg aattctcctt
     2881 cctttacata aaaatcaatt gctttcaaag ctgagacctt tgtatgacca gaaccaaatg
     2941 ttttggtaac ttttttcatc gttaatattt ttttcatttt tattaacctc ctattgctgt
     3001 aattgggtct actttggtta tcgtacgtat cgaaaacaat ccgcctagca ttgcgacaat
     3061 gatcaaaatc ccactatata gcaaccattg tgaccaaaaa acagcaaaag gcatcgcgct
     3121 tggtaaaatc agactagtca aataagcaaa caaaagagca agcagaacgc ctaccacccc
     3181 aacaataaag gactgtgcta ctaaagattt agcgatgaag aaatttttga tcccttgtgc
     3241 tttcattact ccaaaaatag cagtcttctg aagtgtcatc acgtacatga aaataccgac
     3301 cacggcagca accactaaga acaaaaagta tatcatcgca tttaaggtca tattttgggc
     3361 agaatagcca ggaatacttt caatgaatgt cccaatagtt aataactgta gacccttcga
     3421 tgctttttct ttcgaaattt tagctgaatc ctttattacg atcccattga tcggctgttc
     3481 ttcttcagaa gcaaacggct gttgtccata cttcaaagca gtccacgtat ctagggacgc
     3541 atagatgacg ggagaaactg tgtaataagt ttcaggaaag ataccgacaa tcgtcaaagg
     3601 atcgtcgtaa ctgccaattt tgatcgtatc tccaatttta tagcctgaat cagccagatt
     3661 ttgagagatg atgatttcat tctttgtttt aaattcatgc cctttcgtga gttttggtaa
     3721 taaaaatgct tctttcgttg tgccgaaaac tgtaacattt tctttgtttt ttcctttgat
     3781 tgctccgctg tacaatccga ttggtgcttt ctctcctcct tcaatgtatt tcagatctcc
     3841 tcttgttaat tgtgaagctg caaatacttg atttgcatcc tccgacaaaa caatctcttg
     3901 tgcgtcccaa tcatcgattg cttttttaaa ctcttctgaa agtccattcg ctagtcctga
     3961 caacatgaat acgacatacg caatcaagaa cataataccg acgatcaagc catatcgtaa
     4021 tttagaatag cgaatttctt tgatagctag aaacatttgt ttttcctccc tatgatagat
     4081 tgatagtgac aaaatgtcac ttgttgaata gtgtaactct aaagtgacaa tttgtcactt
     4141 gtgttgtttt tttcttatca ataaattgtc taagacatct ttcgataaaa ccttaacatg
     4201 agtcataaaa aatttattta agccttattt atgctttcca tgataaacta aagtgaccaa
     4261 atcaatgagg tgggaaaatg attaaactat tagtagtaga agacgataaa gtattatcca
     4321 ataatataaa agaaatactc gctgcaatcg gcgagataac acaagtttat gatggtagtg
     4381 aaggactgtt cgagttggaa agtggagttt atgatcttgc aatcttggat ttgatgatgc
     4441 cggaaatgaa tggatatgaa gtgttagcaa aaatacggaa agaaaaaata cagactccag
     4501 tattgatttt gactgcaaaa gatagtttgg aagataagat tgaaggattt gaacgaggtg
     4561 cagatgatta tctaacaaaa cctttctatc gagaagaact gatcatgcgc gtgaaagcat
     4621 tattgaaacg ctcgctaggt ctctttgatg aaaatgttct gaagtataag gatatcagtt
     4681 gtgatttaaa aagcaatgaa gtcttgtata aagaacaagt tttgcccata caaggaaaag
     4741 agtttgaatt gctcgtttat tttttacaaa ataagggaat cattttgaca aaagagcaga
     4801 tatttgaccg gatctgggga tttgattctg atacgacgat tacagttatt gaagtttata
     4861 tgagcaactt acgaaaacat ctgcgaagta ctggattgga taaagaaatc aaaaccttac
     4921 gtaatgtcgg ttatatactg caaagggtgt gatgcaatga atggagatat tacacgaaag
     4981 caacgtagtc gcttttttgc gacaaatgta gcagcatttg cttttatctt ttttcttttg
     5041 ggattcatta ctttacagat attaaaccaa tctgcataca gagaaacaga tctgtcatta
     5101 gagaaaataa attttgattc tcgaacagta caattagaga tacaaagata tcagcaaggc
     5161 gatccctttt taaacccgac tcaaacggaa caaaacagaa caaacatgga acagccatta
     5221 atggaaccat caaatgaccg ttttaacact caagtgatct tgtggtctaa aaatggtgag
     5281 atattgaata aagcagctat tggtgggcgt ttcaaccaaa tcaaagaatt aaaaatggat
     5341 acgaaaaatc tgaataccat ccaaaatatt gaattaaatg aagacagtgg ctcatctgcc
     5401 ctctctttcc gttcgattac gaaaaaagcg gagacgaatc aagatggcgt tgcctacatc
     5461 caaattttag aaaatacaaa tcaaatcaag aattcattag cgacttttcg cacgattatg
     5521 attttgtgta tgattgtttt ttggattctc tctattggta tcagttatta cttgtctagt
     5581 ctaagcatgc gccctattat tctttcttgg agaagacaaa aagaatttgt tgagaatgct
     5641 tctcatgaat tgcgcacacc gttaacgatt attcaaaata gtttggaaca tctttttacc
     5701 aagcctaatc atactattat tgaagaatcc gaaagcatcg ctcaagcatt gtcagagacg
     5761 aggcgcctca ctggattgac ttcggatttg ctgacaatcg ctcggaatga ttctgatcag
     5821 cagctgcttt caaagcaaat gatcaataca caagaatata tcgaacaact tgttaaacca
     5881 tttcaagaga tggccatcat agatggaaaa gagtttattc tccataacga tgcaacatta
     5941 gaagtaccaa ttgatgaaaa aaaaatccat caagttttgg ttattttgct ggataatgca
     6001 ttaaaatata cgaatccaga agataagatc attttggatt catctgttaa ccataaagaa
     6061 tggctgattc acgtaaagaa tactggccct tctatcagtg aagaagatca agcaaggatt
     6121 tttgatcgat tttatcggga agatcaatct cgggcgaaag agactggcgg atacgggctt
     6181 ggcttggcaa ttgcaaaaca ggtaatcgaa cagcatcatg gacagatttc tgtatccaat
     6241 tatcttccac aaggagtcga ttttaaagtc caattgccgt tgaagcctaa aaaataatgc
     6301 ttatccctaa aaaagggctg tgacaaaaat cttgtcacag cccttttttg ttgcgaataa
     6361 acggtgttcc agaagtagct tcttcggaaa taagccgaaa tttctttaag ctgaccactt
     6421 ctgtcacaac ctctttagct tacgcttacg attttttatt gcggtcttca atgtgtacat
     6481 caccgcttgt gtcaatatcc aattcttctc ggctaacatc ttctgtcact ttttcaacat
     6541 cttcatgttc ttctttatga atcccaactt cttcacgaat aactgtatgt ttgcttacat
     6601 ctacttgctc ttctttgatt gggatagtaa tcgtgtcatc ttccaatttt tcgtctgttc
     6661 cctgtgaaga ttgatctgtt actggtttgc gttcgatgac gatttcttca cgtttgaccg
     6721 gtacttctac cgtttcagta tcgttgacca catgtttgtg gatgtcgact tctccagttg
     6781 tcacatcatg tgtatttaca tctaattttt cttcttttag ttgaattgta tcatcatcta
     6841 gatttggttt gcgatcgatg tcttcagtct tgcttgtacc cattgtatca gtctcgccag
     6901 gtacgtccat cggatcagtt cctggtgtca tattcaatgg atccgttgta ttttgaaagc
     6961 ctgtcgtgcc acctacgtcc attggatctg ttccagtgcc agtttctaat ccagtcccca
     7021 ttgtgcttgc tgggctaaca gtatcttgtg tatccattgt atcttctgtt gtttctggct
     7081 gacgatagcc ttttactgtg atgacaaatt ttccatcaga aatatctcct ctatattgat
     7141 ataaaggatc attttctggt gtagccgtct cagaatcata ggtatcaaaa gaaaaagtat
     7201 cttttatttt ttcccataaa gaacggtctt cttcaccatc catccgattt tcatctgttt
     7261 caacatctat accagacaat ccgtcaaatc ctagtctaga tgctgcttca tcagtagtat
     7321 aaataacgat atcatctcgt tcataccctt cgttacgcaa ccgttcaatt actgataatg
     7381 cttcttccct cgtactatag cttccgatga ccgttaaatt tttctcttcc atgtaaattc
     7441 ctcctagcaa atagatttat gattggtata ttgttactta taaatttatt ctatagga
//
LOCUS       DACCYH010000117         7390 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.123, whole genome shotgun sequence.
ACCESSION   DACCYH010000117 DACCYH010000000
VERSION     DACCYH010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.123"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(204..1652)
                     /gene="cls"
                     /locus_tag="IVY85_002649"
     CDS             complement(204..1652)
                     /gene="cls"
                     /locus_tag="IVY85_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8045611.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1804..2697)
                     /locus_tag="IVY85_002650"
     CDS             complement(1804..2697)
                     /locus_tag="IVY85_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8045612.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="IVY85_002651"
     CDS             complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="IVY85_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP8045613.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3472..4573)
                     /gene="prfB"
                     /locus_tag="IVY85_002652"
     CDS             complement(join(3472..4509,4511..4573))
                     /gene="prfB"
                     /locus_tag="IVY85_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP8045614.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4699..7233)
                     /gene="secA"
                     /locus_tag="IVY85_002653"
     CDS             complement(4699..7233)
                     /gene="secA"
                     /locus_tag="IVY85_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP8045615.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
BASE COUNT         1975 a         1639 c         1289 g         2487 t
ORIGIN      
        1 gctatactgg ctttgaaatg tgtctctttt aatagcatat attagcttat ttcttgcttt
       61 ccccaaaaaa caagagatac cccaaagtta atttcgctca tgcgagacgc tttctccatt
      121 tctgacgtac tctcagaaat ggccccgtta aaaaaggaca cgagatctcc atctgttgat
      181 ggttctcatg tcctttttat gttttaaagg agcggcgaaa tcaatcttgc taatccttct
      241 ttcatcttga tgattattcc tcggttttca tacatttctt ttgtgagatg cgtagaaatt
      301 ttcgagtctt caaaaaaagc ttttcttaca cgtgaagcca ttcgttcatc ataaaccagt
      361 gtattgacct caaagtccaa gcgaaatgaa cgaacatcga tatttgctga gccaactgaa
      421 acaatgccac catcgataat catcgtcttt gcatgaataa atccattctc atatgtttcg
      481 atccttgctc catactcgat cagctctgca gcgaaagaat acgttgccca gtaaacgaga
      541 agatgatctg gtttgttagg tatttggata tgaactttta cgcctgaaag aagagccaac
      601 ttcagtgctt catgaatcga tccatccggt atgtaataag gcgtttggat caatatctcc
      661 cgtttggcta acgaaatcat tttcaaataa gtcattttga tttgttcatg ttcggtatct
      721 ggtccgcttg tcacgatttg aacagctata tcaccagtag tgtgaatgga cggaaaaaat
      781 ttgggagaat agcctacttc ttttgtatgc tgagaattcc agtccatcaa aaaacggttt
      841 tgcaaggtat acaccgcttc tccgtaaata cgcaaatgat tgtctcgcca ataaccaaat
      901 ttctttacca atcctagata ttcatttcca acattgaacc cacctgtata tccgatttcc
      961 ccatcaataa ctacgatttt acgatgattg cgataattga tccgaggatt aatatacgga
     1021 acaaatagag gaaagaaaaa tgcgacttct ccgcctgctt ttttcaaatt ctgaaaaaag
     1081 ctggaagaaa cttgtgtgga accccatgca tcaagaagga ctctgacttt tacccctcgt
     1141 cttgccgcct ctgtcaatgc atctcgaact tcttcaccta atgcatcact gcgataaata
     1201 taatactgga agtggatatg gtcttctgcc tgcttgatat cttcaatcag cgcatcgaat
     1261 ttttcacgac catctgtaaa taaagtgatt tcatttttag tcgtatagag cgtactttca
     1321 aataccgtga gcatatagat caattgtttc acatctactt gtccagtagg cggatgagga
     1381 taaagccctc gcatcaatgc ctgtttgtct tcttcaattt ccaggttctt accgattttc
     1441 ccttggatct tcaaatcaaa gattttctct ttggaaatcc ctcttcctaa aaaaatatat
     1501 aaaataaatc ctacaactgg aatgaacatc aaaacgagca gccatgccca tgtttgagca
     1561 gtttcctttc tttctcgaaa aacgatgata aaggaaagca aaatattcat aatcaaaagg
     1621 atcgtaaaaa aattgtcaac gataattttc acggcttcac ctcttcttct accaacttaa
     1681 gaatacccaa gttgtttttt aaagtaaagg gaaaaaagcc gcttccttct attctcctag
     1741 ccaataaaaa aagaaagctg gaacgaaact gtccaacttt ctcattttat ttatattgtg
     1801 gtattagaat ttcaagaaac gtctcataga aatgatcgag ccaagtgaac caatgatcac
     1861 accaatgccg accattaaaa gattgatttt ccaaacgata ttttgtggat gaatcaatga
     1921 ataatgggaa cgtaataatg atggattgat caattggaaa aaccatgcat aaccaaatgt
     1981 gatgatcaaa attggaagaa ttgcaccgat cagaccgatc cagccacctt caaggaaaaa
     2041 cggccagcga atatatccat tttttgcacc gactaaacgc atgatttgaa tttcacgctg
     2101 tctagacaaa atcgtgatcc gaatcgtgtt cgaaatgaga aacatcgcta cgaacagcag
     2161 caatgccgct gcaactaatc cccatgtccg aactgcacca gcgatttgga aaatccgatc
     2221 tgaggaagct ccaccgtaat cagaacgaga aacattcttc aaatctgctg cttcttctgc
     2281 tacttgtttt gtataagaag gatcagtagc acttactaca tacacatcat ataacgggtt
     2341 attgtcacct tcaaacaatt tccaggaatc acccatttcg ttttggattt ttttcaattc
     2401 ctgttctttg cttgaaaatt caactttttt aacatgatct aagtcattca attctttttc
     2461 tagctttttc atctcttcgt cttttgtacc gatatctacg aatacagata catcaacgtt
     2521 tccttctata tcctgagcta atttagttgc attcatgata actgccatga atacaccgac
     2581 taaagtcaac gtgatcgtta ccgcactgac tgaagcaact gtcatccagc cgttacgttt
     2641 caaactcttg atactttcta ataaatggcg gaaaaaggtt ctaatcatcg tatccatatt
     2701 ccccttccgc ttgatctcga ataatgcggc cgttttcgat agcaattact cggtgccgaa
     2761 tcgtatttac gatcgtactg ttatgtgtag ccatcacgac agtagttcct tgggcattga
     2821 tccgatctaa taatttcatg atttcccatg agttttctgg atccagattt cctgtcggtt
     2881 catccgcgat aagtacttta ggcgtattca cgattgcccg agcaatagag acacgttgct
     2941 gttccccacc ggataattcg ctaggaaaga ctcgaacttt gtgtttcaaa ccaacaaggt
     3001 ccagcacttc cattactcgt tttttgatat ctcgaggttt tctgccgata acttgcatag
     3061 cgtacgcaac attttcatat actgtttttc ttggtaataa tttatagtct tggaacacca
     3121 cgccgatttc tctgcgtaga taaggaactt cgcggttttt gattgctatt agatcatgac
     3181 ctgcgacgtt caaagttcct tttgaagctt tttcttctcg atacatcaat ttgatgaatg
     3241 tagatttacc ggctccagac ggaccaacca catagacaaa ctctccttgt tcgatgacaa
     3301 tcgagagatt gcggatagcg gtcgtaccat ttgagtactt cttcattaca tccttcattt
     3361 caatcatggc gttctctcca tatctttttc agtctttagc tattatagca tggcaatgtt
     3421 tcaacatgct tgcaaaatct tacaatttta tttcaattta acaatgggtt cttattgtag
     3481 atgcattttt aaatagccgt caataaataa atcaaggtca ccgtccataa ctgcttggac
     3541 atttcctgtc tctacgtttg tccgatggtc ttttaccata gaatagggat ggaaaacata
     3601 agaacggatc tgtgaacccc agccgatttc tttttgttct cctcgaagtg cagctgcttc
     3661 ttgttctttc ttttccactt ctaattgata aagcttagct tttaacatcc ccatcgcttg
     3721 ctcacggttt ttcaattgtg agcgttgtgc ctgactagct actactgttc ctgtaggcaa
     3781 gtgagtgatc cgtacagcag attctgtttt attgatgtgc tgaccacctg caccacttgc
     3841 tcggtacgta tcgattttca aatcatctgg attgatttct atatcgatcg tttcatcaag
     3901 ctctggcatc acatctaccg aacaaaaaga agtatgacgt cgtttcgctg agtcaaaagg
     3961 cgaaatccgt acaaggcgat gaacaccttt ttctgatttt agataaccaa aagcgttatg
     4021 tcctttaatc agcagggtca cactcttgat ccctgcttca tcacctgctt gataatctag
     4081 tgtttctact tgatagccat gctgctctgc ccaacgtgta tacatacgta aaagcatgct
     4141 gccccagtct tgggactccg ttccgccagc ccctggatgc aattcgacga tcgcattgtt
     4201 tcgatcataa ggtccgtcta acagcataga taattcatac gtcttcattt tttcatctaa
     4261 tgcattgata cgtgtttctg cttcttcttg cattgctgca tcaggttctt cttgcagcat
     4321 ctccaccatc aattcgattt cttctagttc ttcggcaaga gaatggaatt ggtcataggt
     4381 ttctttatta gcgttatttt cgctgatcac tttttgtgcg gcttcgctgt catcccaaaa
     4441 accaggttca gccatttttg cttctgcttc cgcgatgtct tcttccagct gatctaagtc
     4501 aaagagacct cctgaaactt gtgacctttt cctgcatttc tgtcaggaga tttcgaattt
     4561 cggatatttc catgtttttt ccttctttca ctatactttt ttcattatat ctagtctaac
     4621 agaatcagca aataacagaa agaaggtgag acgaattctt aaaaaaagaa tttgtctcac
     4681 caatcctgct gtcagaaatt agtttgcatt ttttccatga cagttcttat attttttccc
     4741 gcttccacaa ggacaaggat catttcttcc gaccttattc acatggattg gctgtttttt
     4801 cgcgctagta ttactatttt ctttggcatc tccgtcttct gagtgagaag ctgttccttg
     4861 agaaacctgt tcacgctgta cgttttgacg gatctcagct ttcataaaga ttcgagtgac
     4921 ttcatactcg atggcgccta ccatatcttc aaacatctta tagccttctg tttgatattc
     4981 gactaatgga ttgttctgtc cataagcacg taagccgatt gactgacgta attgatccat
     5041 tgcatcaata tggtctgtcc atttagagtc cacaacacgc aagatcacga ctttttcaaa
     5101 ttccaacagt tgctctggac catttagttg agccgctttg gtattaaata cttcttgtgc
     5161 tcgttcgttt agatactctt tgatctcctg agctgttttt ccttcgatat ctgcaagagt
     5221 aatgctatct tcatggacaa gagtactgcc ggcaaaatcg atgatacttt ccaaattcca
     5281 attttctttc tctaattgtg tatgcgcatc tactacacga ctgattgtcc gtttgaccat
     5341 gttcattagt ggcttggata agcttttttc ttccatgatt acttcttgtc gctgtttata
     5401 gattacttca cgttgttcac gcatcacatc atcgtattgt aagacgtttt tacgtgtgtc
     5461 atagttattt ccttctacac gtttttgtgc agattctact tgtttcgtca gcatcttgct
     5521 ttggataaca gcatcttcct caccgatctt catccgatct aagaaagctt tgattcgttc
     5581 tgaaccgaat cgtttcatca aatcatcttc caatgacaag tagaactgtg acataccagg
     5641 gtctccttga cgtcctgcac gtcctcgaag ctggttatcg atacgacgag attcatgtcg
     5701 ttctgtcccg ataactgcta gaccaccaag ttctcttacc ccaagtccta acttgatatc
     5761 tgttccccga ccagccatgt tggtagcaat cgtcacagca cctttttgac cagcattcat
     5821 gatgatctct gcttctttaa agtgattttt cgcattcagc acttcatgag gaatacgttc
     5881 ttgattcagc atatctgata ataactcaga tgtttcaacc gcaacagttc cgactaacac
     5941 aggttgacct ttgcggtagc gatctttgat atcttgcacg acagcgtgga acttgctttg
     6001 taatgttggg taaagcaaat ctggacgatc atcacggata accggacgat ttgtcggaat
     6061 ctggtatact tggatgttat aaatttctct gaattcttct tcttctgttt tagctgtacc
     6121 agtcattccc gcaagtttct tgtacatacg gaaatagttc tggaaagtga tcgtcgccat
     6181 ggttttcgtt tcgtcttcga tctctacgcc ttctttagct tcgattgctt ggtgaagtcc
     6241 atctgaataa cggcgtccat ccatgatccg accagtgaac tggtcaacaa tcagtacttt
     6301 tccgtcttgg acaacataat cgatatctaa aagcatgata tagttggctc tcaatgcttg
     6361 atccaagtga tgggtcaatg cagtattttc aatgtcgtat aagttatcca aaccgaatgt
     6421 ttcttctgct ttttcgatcc ctgcttcggt taaactgatt gtttttgatt gtacatcgat
     6481 tttataatct tcttcttctt tcaaacgttt cacgaagttg tctgctcttg tatataaagc
     6541 tgtggatttt tctgcttgcc ctgaaatgat cagcggcgtt ctcgcttcat caatcaagat
     6601 tgagtcaact tcatcgacaa tggcataatt cagcggacgt tgtaccattt gatggcggta
     6661 aacgaccata ttgtctctca agtagtcaaa tcctaattca ttattcgtac tataagtgat
     6721 atcacaatta taagcctcgc gtttttcatc cgaagatttt gagttgatat tcaaccccac
     6781 tgttaatcct aagaaattat aaagttcgcc catttccgtc gagtcacggg ttgctaaata
     6841 ttcattgacg gtaactacgt gtaccccttc gccagtcaga gcatttagat atactggcat
     6901 cgttgcggtc aaggttttac cttcaccagt acgcatttca gggatatttc catcgtgaag
     6961 cacgattcca cccatcaatt gtacgtggta tggatataaa cctaatacac gttttgctgc
     7021 ttctcggacg actgcaaaag cttccggaag taattgatct aatgtttctc ctttttggta
     7081 tctgcccttg aattcagcag tttttgcctg taattgttca tcagttaatg ctgccatttg
     7141 gtcagcgtat gcttcaactt tttttgcaat gctatctaaa cgttttagtt ctttcttatc
     7201 gttttcgata agtgaacgaa tcaaattggc catgcgttgt gtaatctcct tttccttctg
     7261 tgtattatcc agactatgta gactttactt ttcattttat cattagttat agataattga
     7321 aatatcttaa gcgtattaaa gtaagaactt cataatttat tcatatttca tctaaaagag
     7381 gaggaaactc
//
LOCUS       DACCYH010000118         7016 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.124, whole genome shotgun sequence.
ACCESSION   DACCYH010000118 DACCYH010000000
VERSION     DACCYH010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.124"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            38..223
                     /locus_tag="IVY85_002654"
     CDS             38..223
                     /locus_tag="IVY85_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045616.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            728..922
                     /locus_tag="IVY85_002655"
     CDS             728..922
                     /locus_tag="IVY85_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045617.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            1113..1362
                     /locus_tag="IVY85_002656"
                     /pseudo
     CDS             1113..1362
                     /locus_tag="IVY85_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1606..2436)
                     /locus_tag="IVY85_002657"
     CDS             complement(1606..2436)
                     /locus_tag="IVY85_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP8045618.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            2644..3978
                     /locus_tag="IVY85_002658"
     CDS             2644..3978
                     /locus_tag="IVY85_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8045619.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4019..4930
                     /locus_tag="IVY85_002659"
     CDS             4019..4930
                     /locus_tag="IVY85_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8045620.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            4927..5781
                     /locus_tag="IVY85_002660"
     CDS             4927..5781
                     /locus_tag="IVY85_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8045621.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            5778..6395
                     /locus_tag="IVY85_002661"
     CDS             5778..6395
                     /locus_tag="IVY85_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP8045622.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            6397..6915
                     /locus_tag="IVY85_002662"
     CDS             6397..6915
                     /locus_tag="IVY85_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP8045623.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         2106 a         1207 c         1402 g         2301 t
ORIGIN      
        1 cttctataat aaaagttatt taaggaagtg tctatgaatg agtaaaaatt ctaaaggatt
       61 cctaaccatc ttattagctt ttatagggta tatgttggtt gggctgctaa aatcatacag
      121 taatgagttg ctgaattttt caacatttat taatgataca ttagttccaa gtttgttttt
      181 cattgtattt tttgctgttg gttattttat aataaagata tgatgactaa tttagctcaa
      241 ctattccagc gtcagctgaa gatattgcaa ttcccgattc cttaatcatt tatctggatg
      301 agacagaaag tgaagttgca acagatgtgc atgtagttga ggataaaaat gatagtttaa
      361 aaattaatag ttcagatact ttactaagag taagtcctat cttttcagct gttatttaga
      421 tgcaagtagc aactaagtta ggtagtactc ctgcaatgga taggtatttg tgtgttcgat
      481 atataagtgg tcgaagtatc aaggttatgt atactggatg ggagatagaa gagttaaaaa
      541 ttggaggtca ccgccacaaa tggaatttag atttagaggt accttagatt tggtatagtt
      601 attaaacaca agatggcctg gtttgtaggg aaagtcgaag agtatttttc cacttctgcg
      661 cccctttaat ttttctagta atgccgtgaa tttgtgatta attggaaata tgaagaaagg
      721 agagtctatg aaaacaacac tagttctatt ctataaaaag catccttatt ttacacttct
      781 gataaatata ttgttggcta gtgttatcgg gatttctgtt gaatatctaa taaataaaga
      841 tttcataggt tcttgttttt atacggcttt atttcttgga cttcttgaag cattttccat
      901 ttataaaaaa tcaaaaaaat aaaatagatt gccgtaagag atctttttca tttcattttt
      961 ttctgaaaac ccgttgtttc tgtcagtgaa gcagcgggtt tttagattta ttgtctttag
     1021 acctaattga gcgcgccgag cgattgaaat acaaaaccac ttgctatgcg ggtggagcaa
     1081 taaagggaaa tagtttagcc cgtacaaagt agatgtgtaa ataccacatt gtattcacac
     1141 caaagtatag aagaaaaata atttataatc aatatcgagc caatattcag gagatcatca
     1201 agagattgtg caaatctaaa atataaattt gggtatcgac acttttgggt ggaaggatac
     1261 tatgtgacaa aagtaggaca aaatgaagcg accatcaaaa agtacattca agaacaagaa
     1321 aaacatgata gtgcgttaga taagctgagt gtacgagagt aagacgatcc ttttaagggc
     1381 taaggagtag tacataaacc ttttaagagg tgggcaaaac tggcaaaggc aatatggctt
     1441 gaacataaaa tatgaaagcc agcgtcttta ggcgctggcc ggtgatttga gcttatagct
     1501 ctagagaaaa ccaccctttt gaagggtggt tatgattaat gactttagtc agttttgctc
     1561 acaaagcgtg tggaacaaaa aacgggtgtt tctacgggta gatatttatt tcttgaatgg
     1621 aggtgtccca tacatttttg gtgttactgg ttctaatttt ggtgcagcgc cttcgatctt
     1681 aggttctttc aaccattgat attcgccttc gccatccagt gctttgccac tagcccaaga
     1741 accttcacta ctttggtctc cctctgagaa gttatagaag tcataagcaa tgtcttttcg
     1801 ttctttgtca cgcgggaaag tacttgggac taagattgaa ccttcttttt cttctaattc
     1861 tttcactgca cattcccata gattttgatg atagctatct cttgcaagga gaacagacag
     1921 catatctttt acgccacgat catcgatcat ttcgtaaata cgtgctactt gaagtcttcc
     1981 ttgtgattcg gcatttaaat tagctctaaa gtctgctaac aagttgccac tcgcaatgat
     2041 ataggcaccg ctccatggat tgcccacgct gtcagcagga cgaggtccta agccagctac
     2101 gatcgcgtgc tgtggattca ttccagagat aatggcaccg attgttggat ctttttgcat
     2161 cgctgcttct tgatcttcaa caggcgcacc ctctaataaa cgagcaacca ttgtcgccaa
     2221 catttccacg tggccgattt cttctgttgc aatgtccatc agcatatctt tatatttttc
     2281 ttctcctcgg cagctccagc cttggaacag gtactgcata gctacactca tctcgccata
     2341 ttggccgccg atcagctcct gcagatattt cgcgattaat ggattcggac gatctggttt
     2401 tgcttcaaac tgcaattctt tctggtgtct aaacatcaaa attcctccta atctattttt
     2461 aatacattct cattctgcta cgaaagatta aaaaattcaa agaaagcgct cgcgaattat
     2521 ataaagtatt ggtatcatta ataatttttt tagaatcata ccttataaat tgttattttc
     2581 agaacaaaat gatatcgtta tattaaaacc ggcatcggtt acattaaaat ttggaggtaa
     2641 aatatgaaaa aattattgtt tacagggttg ctggcaagtt cagcattgtt tttattagga
     2701 ggatgcggat caagtaatgc aagcgatgcc agttcaagtg cagcaaattc ttctgaaaaa
     2761 acaagtgatg gaagaaaaaa tattgatttc tggtcctttt ggggatccgg tgcacgaaaa
     2821 gaagctatcg aagaaatcat tgaggatttc aatcaatcac aaaacgagat ccatgtaaat
     2881 tataagtatc agccatgggg tgacatctgg actaaatctt tatctgcaat aacagctgga
     2941 aatgcacccg atgttattgt acaggacata aattccgttg cccaaagagc agaagcacag
     3001 caagcaacta atctctccaa atatgtagaa aaagacactt ctaaggattt ttatccgcag
     3061 ctttgggata cagtagaata tgaaggtgat ccttatgcga tcccattcaa tacagataca
     3121 caagtcattt tttataataa aaagttattt aaagaagctg gaatcagtga acaagatcta
     3181 ccgacaactt gggatgaatt agaagaaaca gcacgtaaac tagataaaaa agaaggaaaa
     3241 gaatttacac gcatcggttt ttatccatta tggaatttag gggctgatgt atgggcgcta
     3301 aacgcagata acgggactag ttggtttgac aaagatgaaa aagtcaagat tgatactaaa
     3361 aataaagtgg aagccttgga gtggatccaa gactggcaag attattatgg gaaaaatacg
     3421 atcaataaat tagaagccga atttgggtct ggtgtatctg atccatttat ttccggatta
     3481 gtagccatgc gtgcccaaaa tatcaattat tactctagtt tgatggaaaa tgcaccggat
     3541 gattttgatt ttggcgtgat gcagctccca gaaaaagaag aaggttcagg ccattggtcg
     3601 tggggtggcg gattcgtctt ggaagtacca tacggagcaa aagatcctga ggcctcttat
     3661 gaattcatca aatatctttc ttctccagaa gtacaagaaa aattcggcga aaaaagtttt
     3721 gatatcatgg cgagcaagac agccaatgag aatttagtga ataatgacaa cctaacggat
     3781 aaaggaaaaa tgatctatca aatggcggac gaaaacttta aaaatacggt gatcacacca
     3841 gttcctttag atgctccgga ttacacgaca ttagtgaatg aacagattga ccagattctt
     3901 ttaggaacaa aatcaccaaa agaaggattg gcagatgcac aaaaagcagt agaaaactta
     3961 gtcaagcaaa atcaataatc catttttgac tgtctaggta cagaggaggc aagaaaattt
     4021 gaagcgttta tctatccgaa agaaaaaaga agcaagatgg gcctatttat ttatctcgcc
     4081 ttttattatc gggttcttgg tatttatgct agggcctatg cttttttcag ttattggtag
     4141 tttcaccgat tataatttga catccaagat gaatttcatt ggtctagcaa atttcaaaag
     4201 aatgttcgtt cacgatgatt tattttggaa atcgttatac aacaccgttt atttcgtcat
     4261 cttcaatgta ccgctgacga cgattttcag tgtgttttta gccacagtac tcaatcaaaa
     4321 aatcaaagga atcagtgtat tccgtacagc tttctattta ccggcagtct tatcaggagt
     4381 agcagtctac atcttatgga tgcagctgct ttctccatct gcaggattga tcaacacatt
     4441 tctgggctgg ttccatatcc ctggaccagc ttggctgttt gatccacaat ggacgaaacc
     4501 tgcgttgatt ttgatgaaaa tatggagttc tggaggagca atgctacttt acttagctac
     4561 attacaaaat gttcctagat ctttatatga atcagcagaa atcgatggag caggcgtttg
     4621 gggaaagttc aaaagtatta ctttgccatt aatcactcct attatttttt acgatgtgat
     4681 cacctctacg atcggttctt tccaaatttt tcaagaagct tatgtcatga cgaaaaatgg
     4741 aacaggcgga cctgcaaatt ctttgctttt ttataatctg catatgtgga acaaggcgtt
     4801 cgtagctttt gatatgggtt atgcgatggc tatgtcaatg atcctgtttc tgatcgtgtt
     4861 ggttctgacg tttgtcaatt tgaagcttgc acctaagtgg gtctattatt ctggaggtga
     4921 cggcaaatga tggattattt caaaaagacc agtgtgaatc ggcaaacaaa aaaaatacta
     4981 agttatgtcc tcatgacagt aataggaatc attttgatca ttccattgct ttggatggtg
     5041 tttacttcat tgaaaccaat ggaagaaatc gttcgttatc ctccgacctt tttcccagaa
     5101 aaaatcgtat gggaaaacta tctggatacg attgccgcct ttcctttttg gcgctatgca
     5161 agaaatacat tattcatcac tgtgctagta gtgatcggta atgtcctttc aaattctttt
     5221 attgcttatg gatttgcgaa gcttgatttt cctgggaaaa agctgatgtt tgctttggtt
     5281 ctttctacga tgatgatccc ggggtttgta acgatgatcc ctcaatatgt attattctcc
     5341 aagatcggct gggtaggtac ttatttgccc ctgatcgttc catcgttctt cggcaatgcg
     5401 ttcaatatat ttctgatgcg tcaattttac ttatcgatca ataatgaatt gatcgaagct
     5461 gctgaaatcg acggagcgaa tcatttgtat atttggagtc atttgatgct tccattgacg
     5521 aaacctgcct tgattaccat tgcgatcaat tccttcaacg cagcttggaa tgatttctta
     5581 ggtcctctgt tatatatcca agatcaagaa aaatacacgt tacagattgg cttgcaagtt
     5641 tttcagaacc aagcaacgac acaatggaat tatctgatgg caggagcaac acttgtcttg
     5701 atcccgacga ttttgctgtt cttttttgca caacgttatt tcattgaagg gatggatttg
     5761 acaggaggtt caaaaggatg atgaatcgat cgattggtaa aaaagccaat gattatttct
     5821 tagaaatcat tgaatggatt ccacgtctta tccatttaca gctactatgg ttcctgtgcg
     5881 tcttgcctgt tttcacatta ggaactgcaa gtcgaacagt tctgtatatg atttatcatt
     5941 acaaaaaaaa tgaagagaaa aagctcggtt cactattctg gaaaaagttt cgtgagaact
     6001 tctctcttta tggaaagcaa gatagtttag caagtgttta ttttcttctt ctattgatcg
     6061 attatcagat tttccgctat tggggtgggg gaattggcta tacattgatg tatactacgt
     6121 tgtttttgct tctattaagc gcgcttctct ttagttacaa aatcttgctt caactggaac
     6181 aaaaagaagc ttcttggatt actgcatttt tcttgttttt taatgatttc aaaagcgcgc
     6241 ttttctattt ggcaggcacg ttgatccttc ttgtcgcatt ctggtttgca ggaccaatct
     6301 attttgcttt attaggtttc agtttcttgt tttattttca agtcctgctt tttatccatc
     6361 gtacgcaaga aaaggttctt aaaaaggagg aataacatgt tgcgtttgat tgctaaaatc
     6421 gatgatagta ttatcaacaa gttcgccaag caacggaaaa cggatagtcg aaaaatcaat
     6481 tttttgaaat ttatcagtaa tatctctaat ggtggtttta tttggttgct ccttgctgct
     6541 gttttagtct tgatacctga tactagagaa tatggtctag ccattcttat cggcagtttg
     6601 atctattttt ttgttggttt gctttttatc aaaaaaacga caagacggaa aagaccttat
     6661 gaaaaaaatc gtcggcttgt cctttacaca agtaaaccga agacttcttc ttttccttca
     6721 ggacatacag cgctagcctt tttatcagta gctgcgttgt tcgaattaag tccagttgca
     6781 ggaatattag cctttttatt tgcttgtttg atcgcttatt cgaggatttt tttactttta
     6841 cattatccta ctgacatttt aggaggagct attttaggaa gtgttattgg catcctatgc
     6901 gctgccgtta tctgaattaa ttgtacgaat aaagcatgag tgcgtaaatg cgcatttgtg
     6961 ctttattttt ttgtttcgag gaaagcgtag ttgtttattc atttgtctta ataact
//
LOCUS       DACCYH010000119         6791 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.125, whole genome shotgun sequence.
ACCESSION   DACCYH010000119 DACCYH010000000
VERSION     DACCYH010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.125"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            25..915
                     /locus_tag="IVY85_002663"
     CDS             25..915
                     /locus_tag="IVY85_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP8045624.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(988..1812)
                     /gene="nadE"
                     /locus_tag="IVY85_002664"
     CDS             complement(988..1812)
                     /gene="nadE"
                     /locus_tag="IVY85_002664"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP8045625.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1824..3296)
                     /locus_tag="IVY85_002665"
     CDS             complement(1824..3296)
                     /locus_tag="IVY85_002665"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP8045626.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3933..4295)
                     /locus_tag="IVY85_002666"
     CDS             complement(3933..4295)
                     /locus_tag="IVY85_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8045627.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4518..5270
                     /locus_tag="IVY85_002667"
     CDS             4518..5270
                     /locus_tag="IVY85_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8045628.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5410..6333)
                     /locus_tag="IVY85_002668"
     CDS             complement(5410..6333)
                     /locus_tag="IVY85_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8045629.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6348..6620)
                     /locus_tag="IVY85_002669"
     CDS             complement(6348..6620)
                     /locus_tag="IVY85_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045630.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2075 a         1352 c         1160 g         2204 t
ORIGIN      
        1 aaaacctttt aagaggtgat atatatgcaa ctacaaataa acgaagaatc cttaccggta
       61 tatgaagcat tagctagtaa aacaaggatc aagatcatcc agctactttc caaaaaaaag
      121 atgaatgtca aagatttggc aaaagaactt ggagtgagca gtgctatcac gacgatgcac
      181 gtgaaaaagc tagaggaagc aaacatcatc aaaacagaaa aagtaggaca acaaaaaatt
      241 tcgagccttc gtgtcgataa aatcgatatc agttttccag aaaaaatatt caatgctttt
      301 gatacgaaag aaacatcgat acctattggt cactatacaa attatgcaat tgaaccaaca
      361 tgcggtttag ctacgatcca tgattttatc gggaaagtgg atgaaccacg ctattttatg
      421 gatcctcgaa ggatggatgc ccgcattctc tggtttacca gcggatttgt cgagtatcag
      481 gcacctaatt ttttaaatac agaggataca ttggagatgt tggaagtttc tgtggaaatt
      541 tcttctgaat ttcctttttc aaatgacaat tggccttcag atatcacttt cagcctaaat
      601 ggtgtggagc taggaacatg gacgagccct ggagattttg ctgatatccg cggtaaatat
      661 acgcctgatt ggtatccaga taatcttaat caatacggct tgctgaagac gatccgaatt
      721 accaaacact tgacgaatat gaatggagaa cccttgtcaa acatcacgat caacgacatc
      781 cctaaagagc aagacacttg gcatttgcga atcgaagtga aagacgatgc caaacatgtg
      841 gggggctgta ctttatttgg taaaggattc ggtaattacg atcaggatat caaattaaaa
      901 gtttactaca gctgatattt ttcttttcta ttttataaaa caaaaacagc caatccctat
      961 gaagggacgg ctgtttttgc tttattgtta tttccagaaa tcatccaaaa cggaaattgg
     1021 caaatgtcgt ttatgttgtg tcttgttcca ccatgcttcg attttttctt gtgcttgagc
     1081 gggaacttct tttccttcta gataatcatc aatttcatta tatgtgacac ctagtgcgac
     1141 ttcatctgca atcattggtt tcccatcttc aagatctgca gtcggaattt ttgtgtataa
     1201 tttttccggt gcattcaatg cttgaagcaa ttgtttccct tgtcgtttgt ccaaacggaa
     1261 taatggcaaa atgtcggctc caccatcacc aaatttcgtg aagaatccag taatgttttc
     1321 tgcagcatga tctgtcccta atacggctcc tgctctttct ccagctacgg catattgcgt
     1381 aatcatacgt tgacgtgctt tgatatttcc tttgttaaaa tcgctgattc ccacacctgc
     1441 ttcgcttaat acttgtactt gtgcatcaac agctggtttg atatttactc gcagactaac
     1501 atcgggctgg ataaagtcaa gagcagcttt tgcatcttct tcatctgctt gttcaccata
     1561 aggaagacga acagcgataa attgatagtt ttcatctttt gtttcttcac gcatttcttc
     1621 catcgttagt tgcgccaaac ggcctgctaa agtagaatct tggccgccgc tgatacctag
     1681 tacaaaagtt ttaagaaacg gatatttttt caaatatgct ttcataaagt caatactttt
     1741 acgtatttct tcttttggat caattgttgg tttgacacct aattcattga taatttctgt
     1801 ctgcaggctc atttattctc ctcctatgct tctgtatgaa gttctgctac gctttttttc
     1861 attcgttcca tgatagccat tttatggttc cagcattcag tagacagatc gactgggtat
     1921 ttttgcggat tcaagtctcg tttatactct tcccatagtg aatctagatt ttcttttgta
     1981 tattctttga tttcgtttag tgcaggtaat tcgtaaactc tttttccttc gataaagata
     2041 tcttgtaaaa ctgggcgagc tgtaaagtct cgaacagttt tattgataaa cgtatgcact
     2101 ggatggaaca tgaagatagc ttcttcttct cgagggtctt catcccataa tgtcacgtag
     2161 tctccttcag atttcccatc aaaatttcga gtgatgcgcc acacttgttt tttccctgga
     2221 gtagttactt tttctgcatt gcttgataac ttgatcgtat ccatcatttt tccttcatca
     2281 tcttcgatcg atactagctt gaatactgca ccaagggctg gctgatcata ggctgtgatc
     2341 aattttgtcc caacacccca tacatcaatt ttagcttttt gcatctttaa attcaaaatc
     2401 gttgcttcat ccaagtcatt cgaagcatag atttttgctt ccgtaaatcc ggctgcatct
     2461 aattgttccc gaacacgctt agatatatag gccatatctc cactgtcgat gcgtacgcct
     2521 tggaagttga ttttgtcacc taattctttc gccacgcgaa tcgcacttgg cacacctgat
     2581 ttcaacgtat cataagtgtc gactaagaac acgcagtctt tatgggtttt agcgtaggcc
     2641 ataaatgctt cataatcatt tccataagat tggactaacg aatgagcatg ggtcccgcta
     2701 gctggtatcc caaatatctt cccagcacgt acattgcttg ttgcatctgc tccgccgata
     2761 tatgccgctc tagttcccca gactgctgca tccagttctt gtgcccgtct agttccaaat
     2821 tccaatagtg gatcttctcc gatgacagat ttgatacgag ctgcttttgt cgcgatcaac
     2881 gtttggaaat tgaccatatt caacagtgct gtttctacta attggcagtg cgctaaaggc
     2941 ccttccactt ggattaatgg ttcatttgca aaaactagtt ctccttcacg agcagaacga
     3001 actgttgctt taaattcaaa attttgtaaa tactccaaaa aaccttcagg atagacttct
     3061 aatccacgca agtaagcaat atcggaatca ttgaacgtca gattctctaa ataattgact
     3121 aaccgttcca atcctgcaaa aaccgcgtat ccgtggttaa aaggcatttc tcgaaagtaa
     3181 cattcaaaaa cagcatgtaa atcagctcga cctaattccc agtatgtctg catcatattg
     3241 atttgatata gatccgtatg cagcgtcaaa ctgtcgtctg ggtaagtatt attcatcttg
     3301 tattactcct cttggcaagt tttcatttcc tacccattat aacaaaaaaa attaatagga
     3361 aaagaaatct tccatttttt ctgaaaaagc acatgtttta cggatatttc ttgctaaaaa
     3421 tgaactccat ctgctaaaac tatcctttct tattggtatt taacctaaac gagaaagaat
     3481 acttatttta ttcaatgtat ccgagacacg aagaaattag cgttttctat ttttcttact
     3541 tgataaaata agtagatttt cctactacaa tcgtagccgt taagataccg atgattgaac
     3601 aaatataacc aaatgtctct acaacataat caatggcaaa agtttttgaa gcaataatac
     3661 gtctttcctt ttttgcttct tatccccaaa gaaacggata taacttatta tgtattgaat
     3721 ttttttggaa aggcgaatta tgacaagata taccttgcgt tgaaaaaact cgcggaaaaa
     3781 atatcacacg tttttaaaaa gatacaaagt aagaaagcag ctgtcaatag aatggaaaca
     3841 aaaatggcga gacatcaatc agtctcgcct tgttttttat tgtatacatt agatatggag
     3901 caggttacca taaagatgat tattagtcgt tattaaatag aatccttcgt atttttttgt
     3961 tctatcacta caatatcttt atttggacgt ttgaattgta ttccggcacg ttctagctcc
     4021 tgtttcactc ctacatggac tttgacatct tctcgtgtca tttttaaaac gtgatgaatc
     4081 gcatagtcga tttcttgcct tgtgattttt ttcttcgtat tgatcatcac gacttgcttt
     4141 ttatcatatg tgtctaccaa tagcagttgt gttcgatcta acgaaaataa tttgtaataa
     4201 ttaatagccg tattcccaaa aaagaattcc actactttag gataaaatgt cttaatattt
     4261 aaattactat tcacaggtgt ttcgattagc ttcatctcat tcctcttttc tatatagtcg
     4321 atttagatcc aatcttaagt tatgttacca tctttcctag cattaaggaa tcggttacaa
     4381 ttgaaccttt aaagacaaaa ggatttacct tttatcactt aatagtataa cataatttcg
     4441 tgagaaaaaa atacaatttt ttcttgacca tctcgttacg taaaggatta tgcttacatt
     4501 tgtcaggagg aaaaataatg gagtacacaa taaagaaaat gtcagagata tcaggagtca
     4561 gtccgagaac cttgcgcttt tatgatgaga ttggactgct aaaaccagct aggatcaatt
     4621 cttcaggtta tcggatttat ggcaaaaaag aagtcgatcg tctgcaacac atcttatttt
     4681 atcgaacaat ggcattcaag ttggatgata tccaagaggt cttagacaat ccatcttttg
     4741 accaccagaa agctttaatc aagcatcagc aaatgttgct ggaaaaacgg gcacaaatcg
     4801 ataccctctt gacaactgtc caacagacac ttgatatgta cgaaggagga agaaaaatga
     4861 gtgacaaaga aaaatttgaa gggtttaaac aacaaaagtt gaaagaaaat gaagaatcct
     4921 atggacaaga gattcgacaa aaatacggaa aaaaaaaggt tacagcatcc aatgaaaaat
     4981 ggatgaatat ggataaagaa acgtatgaag aaatgcaaac tgttgaaaag cagatgttat
     5041 ttgatctcac tctttactta aaagaaccag aagatgaaag cttagcagac cgtatcttcc
     5101 aagctcataa gaagtggttc tcttacagct ggcctgaata tcagccaaaa gcgcataaag
     5161 gactaggaat gatgtacgaa gccgatgaac ggttcactgc atattatgac gagcgaagtg
     5221 gtcatggtgc agcaaaagct ttaaatgaaa ttatccaaaa aaaggcgtaa tcttttacat
     5281 gatccaacaa aaaagtacac gtaaaatgac ctaataacaa aacagcagac acccaagtta
     5341 gatttttatg ctaacttttg gtgtctgctc cattctttca caagtttttt catcctactt
     5401 taaatatatt catcgttgaa taaacgtata aggtattttc actttatcct ttttttctgt
     5461 aagaaacaat tgaaatgcca tttctccgca agcttccaga tggtggtcga tggttgggaa
     5521 accaagaagc tcgctgatca gcaaattatc gcgtccaatc accactggtg tcttatcttg
     5581 cagatagctt tgcaagatgc ctgctgcaac catatccgaa tttgtaaaga caccatcaac
     5641 tccaagctct ttaaaaaaga agcctgcttg ttttcctttc tctgcatcta tacaatccca
     5701 aaaaacaagt gattcatcaa aagatggaaa aagttgatga catagctgca aagtgatttt
     5761 tgagttataa cttagcctgc cacttcgccc caacgtgatg cccaattgct ttataccttg
     5821 ctgcttcaaa taagtaagac cttctgtaat tgattgttct cgatcaataa agacacagct
     5881 tgcctgtttt cccttgattt cttcacaaaa aactagaggt ccatattttt gatatgccaa
     5941 taaatcatct agtgtattgg cacgagacgt aatgatcaat ccatcgaaag ctttagctgc
     6001 aaattcttca agataccttc gctctttttc tttatgatag cccgtcggta aaagtgtcac
     6061 tttgtaatca taatcaaacg ctgcttcaat gatcccactg atcaactgat caaaatacgg
     6121 atgattggta taaggcagaa tcactccaat gcttttcgtt tttccataac ttaactgtct
     6181 ggcatttgca ttagggatat aatcgacttc tttcatgacc gcaagtattt ctttcctttt
     6241 ttcctcatct acatatggat gtccattgat tacacgtgaa atagtagaaa ctgaataacc
     6301 tgtgagcttt gcgatatctc gaatgtttgc cactgctgct tcgccaccta tacaaaaaga
     6361 ttttcttgta aatagtctac cacagctgga ccgcattcaa aaaactgata catttttcct
     6421 tcatgcgaaa aactgatccg ctgcccgccc caatatatat cttttgtcaa attttcgatt
     6481 tcattcattg gtatttcttt tactatattc ctattcaacc cttgaaaact gtacaacgtt
     6541 aatacggatc ccttgaactc gacaaacgca taaaaataat tgttcgttgt gccatctgtt
     6601 atctctacat aagtattcat tgcaagccct ccttttgata ctttgagtat agtgtatgaa
     6661 acgcgtctca tagcaaggag tttgttttgc caaagaaaag ttttattcct ctttcattat
     6721 taccaaagtc tcttatttta aattgaatac attataatga tactgtaaat atatctttat
     6781 tattagtgaa t
//
LOCUS       DACCYH010000120         6671 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.126, whole genome shotgun sequence.
ACCESSION   DACCYH010000120 DACCYH010000000
VERSION     DACCYH010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.126"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            187..2862
                     /locus_tag="IVY85_002670"
     CDS             187..2862
                     /locus_tag="IVY85_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAP8045631.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3044..3283
                     /locus_tag="IVY85_002671"
     CDS             3044..3283
                     /locus_tag="IVY85_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045632.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3299..4672
                     /locus_tag="IVY85_002672"
     CDS             3299..4672
                     /locus_tag="IVY85_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8045633.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4676..6310
                     /locus_tag="IVY85_002673"
     CDS             4676..6310
                     /locus_tag="IVY85_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8045634.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6473..>6671
                     /locus_tag="IVY85_002674"
     CDS             6473..>6671
                     /locus_tag="IVY85_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045635.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2342 a         1072 c         1338 g         1919 t
ORIGIN      
        1 ataatttaat aaggattgat ttatcgtaaa agaaaaaagt tgtgatttcc aaaatcacaa
       61 cttttttctt ttgtcaagat tttgagtggg atatattttt gtagaactga agatcatgaa
      121 attatgagag gttggaggta atgtagtcat acaaataaaa attttttaga aagtgagtgg
      181 taattaatga aaatgaatca cacttcaatt cagaagcatt tacaactgtt aaatacactg
      241 gggatttctt atgttaattt gagaactttt tctacattaa acaagaaaac cgtttctcat
      301 attttttcta ctgcagattc tcaagcaatt attaactatt tagaaaaaca gaaattgtct
      361 gaaggtacag taaacataac ttataatcct attaaacaag aggtgattac acaaaaaaag
      421 cattctgtga gagacaatga tattgaaaaa attcggtttg tatttattga tattgaccct
      481 aaaaggaaag agggaagtgc aacagatagc gaaaagaaaa aggcagaaaa tgttatggaa
      541 caagtagaac gttatttgaa agaaaagaga atagaatcat ttgtaaaagt tgatagtggg
      601 aatggctacc atatttttct tcccataaat gaacaaccaa ataatcacga gacgattctt
      661 actattcaga attttcttca attacttcac cgaagatttg gagtagaaga tgaagttgat
      721 attgatactt ctgtttataa tccttcaaga ttatgcaaat tagttggtac accagcggtc
      781 aaaggaaagt ccacaaaaga gcgtccgcat cgtttatcta gtttcctttc tattcccgat
      841 aacatagata gaagtaattg ttttaaaatt cttgaacaga tagttgaaga aaattccatg
      901 atgtcaacct ctagagtaaa aagccatcca tcttttagag aagtgttacc ttttattcaa
      961 gcagactata aacaatggtt agctcactat cctgaattaa cttataccat aaaagagggc
     1021 gatcataaag gagttacctt acttatattt gacgaatgtc cattaagaaa acacacgaat
     1081 aattctaatg gttcttgttt gtctgttacg gctgataata agattcgatt tcattgtctg
     1141 catgcttcgg ataaaaataa agatattcaa gactttgtaa aaaaatatcc gttgccaaaa
     1201 cctgtaaatc cttctgtaga atcattagaa tccaatcaag aatattattt tgaccagttt
     1261 aaactgaatc aagaaggagt atttttggtg aaagaaggtc aagaagaaaa agttgcttgt
     1321 ccgatgttta ttaagcaaat acgcaaaaat atcgaacaaa aagtcattac acttgttctt
     1381 gcctattatg tcgatcattg ttgggatgaa atagaaatag aaggtggatg tttacaaact
     1441 aataatttta aacaattagc aaaacatggc ttaatcatta aagctagaag agaagctgaa
     1501 gtcgttgact ttttacagat tcaacgggag aatgctccaa tcttttttga acatgaatct
     1561 cttggctgga aaaaagaaaa tgaagcaatg atttttcagt tacaagattc ttttggacgt
     1621 gaaattcctt ctgtattaac taaaaactca tgctaccaat taacaactaa tggtacagaa
     1681 gcggaatttg acgcattggt tcataagtat gttttaggaa cgaacttaga acttgcttgg
     1741 tcgattggta ttgcttctat tgtgttaggc tatttaaacg ttacgaaggc taatactttt
     1801 ttgagtttac taattaacct acagggcaag agcacaacgg gtaaaaccac tacgttacac
     1861 tttattgcct ctttatttgg aaatccaact acggttctta gaaatatgaa tgctaccaat
     1921 aaagccatta taaagctagc atcaaacaat cagggaggtg ttccactaat actagatgag
     1981 ttaggagcag caactcaaaa taactttagc tcgttgatct atcaattagc ttcaggagaa
     2041 gaaaggctaa gattagattc taattgtcag cttatgccac aagagacttt ttctgttttg
     2101 acccttacct cttctgaaga gcgtttggaa aattatttca atgagaaatt aactggtttg
     2161 aaagttcgat atatcgaatt ttctgatatc aagtttacaa aggatgcgaa tcacgcagag
     2221 aaagttaaaa gtctttcttc taatactttt ggttggggta taaagttatt tcttttgaaa
     2281 ctctttgaac aagggattac aaaaattgaa gcaacttttg tcgaagctca aaaaaaacta
     2341 tgggctgttt tacctgaaca ttctttgaag caacgcattg gtaataatct agctttgatt
     2401 tatagtggag ccctcctaat tcaagatata ctgggctatc ctttacgatt agagcatata
     2461 cagacggtgc ttattaattc gtacgaacag ttaattgtag atactagttt aggaagtgag
     2521 caaacggtat ttgaccgagt gaaaacattg ttggtgatga atagccataa atttttaaca
     2581 aacagtgaca acaaacatac gcccagtacg atttggggaa aagtatcaat tcaaaaaaat
     2641 ggggatatta aggtaaatat ttttcctatc ccgtttgagg acttgttaag gcgagaattt
     2701 caagtaaagg atctaaccta tctttttagg caattgcttg attcgggtaa tatgtgtgca
     2761 gaaaaaggga gaagaacgaa acgtattcac atgaatcgtc aagttcttcc aacatacgag
     2821 attctattac cttcttcact caaatcgtat tttcgattgt aatttagaat aaaccaaagt
     2881 atgaaagtga ggcaccatgt tatggaaata tttcttattg atttagcaaa acaactgatt
     2941 acttatctgt atgatagcga gcaaacccaa acggaagtaa atgaagcttg atctatgctt
     3001 catttacttc attcaatcaa acatgaaaga aaggaaaatt ctaatgacta aaaataaaat
     3061 gacactaaaa gcagaggttc ttctgtatat tcaagaacat ttttcaaatc aagccttttt
     3121 tacaaaacca atttacttgg atttcgaaat aagaggggta tctgctggat ctatcggggg
     3181 tactcttcaa gcgttgaaga acgaaggata tttagaaaat cactttgtgc aacgttcgtt
     3241 caatagaaga gtcgtgaaaa aatggtattt agttcattca tagagagggg aatcaagcat
     3301 gcgtgtggct tgttatgatc gaacgtctgt ttatgaagaa gcgaaaatta gtaatgacgc
     3361 tcaatatgat gcagtgaaag agtatgtttt agagaaatat gaacaaattc cccaacgatt
     3421 ttcagacaaa ggagaatcag gggaaaaagc ggagcgtcca gcttttcgac agcttagagc
     3481 ttgggtttgt gcgtcagaag aagagggggt gatctatgtt tggcgttatg atcgattggc
     3541 acgtcaagcc aaaatagcct tagaatttat tgagttgtgc caacaacatc aggttcaagt
     3601 gtattcgatt tctgaacctc ttcctgcttc tttctcacac gcgtcaacga ataaactatt
     3661 tgtgcaatta ttgttcactt ttgcagaaca acaacgaaac gtcattattg agaatgttcg
     3721 tgccggactt tcctataaaa agcaacaacg gcaatatttg tcttctgcgg taccatatgg
     3781 gtatcgttta gtgaatggga aaatccaaca agaagtacac gaagcaagga ttgttcgcta
     3841 tatttttcaa ctttatttga ccaaaaaata tggctacaaa aagctttgtc aacgattgac
     3901 gcaacaaaag cttttcttcc gcgaacgtcc ttttcagccc tatcatattt atagtatcct
     3961 aaaaaatcca ttgtactatg gcgaaatcaa aggaggatct ttggggaagt atctaggaac
     4021 ctttgaaccg attctctcga aagcaacctt tttacaagcc caagagattc gccaatcaag
     4081 gtgtacagct aaaaaagata cgtatctgta cttattacgt caaaaaattc ggtgtccctt
     4141 ctgtggtcgt catctgagtt cgaagtatca atggaataca aagaagacga agacgcttca
     4201 ttattatcat tgtaccgacc gaagctgcca aggaattttt cttcgggcag aaagggttga
     4261 gtcagaagta ttggaaaaga tacaggcttt ttttcaatac gaaggcgtct atcaagcctt
     4321 gattgcccaa ttaacgcaac aattagccga aaagcgaaaa gatgaaaaac gcaaagtaat
     4381 ccaacaaaag aaacaagaag cacaatggat taaggcattt gaagagggca attgttcact
     4441 ggaagaactc aagcaacaac tgcaagctct tcaaaagccg atgtctactt cttcgtttct
     4501 ttcactccaa cagtgtgaag caaaagtgaa agaatggctt caactaaaag acctctctag
     4561 tcgacaatta ttatgtgact atgtttgtcg gattgaggta acaaaggata aaacagtttg
     4621 tgggatttac ttccgacaac tgacacaaaa tattttagaa aggaacgaat gatctatgga
     4681 aatgatacaa accaaacgag tcgcattgta tattcgcgtg agtacacaag aacaagcttt
     4741 agaaggacat agtattcaag cacaagaaca aaatggacga cagtttgccg aacggatggg
     4801 ctatgaagtg gtagaagttt atgccgatga aggaatcagt gggaaatcca caaaaaatcg
     4861 gttcgcctat caacgaatga tgaacgatgc tcggaaccat cgttttgatc tactagtgat
     4921 ttggaaattg acccgtctcg gacgcaatat gttagatgtc ttacaaacag tcgaggaatt
     4981 attgcgatat gacgtaggat tacattcgat ctcggaacaa tttgacgtga ccacttcgac
     5041 aggtaaattg atgttacaac tccttgggag tttcggtgaa tttgaacgca accaaattgc
     5101 ggagaatgtt caaatgtcaa tgcaaagctt agtgaaagat aaaaagcgtt atgcaggagg
     5161 ccgacgttta ggctatgtca gtggtattga cacgaatggt caaaaacaat tggtcattga
     5221 accagaagag gcgaaaattg ttcgtctgat ctatgcaaaa tatttaggcg gagcagggta
     5281 tcgagcgatt gccaattttt tgaatcgtca aggatatcaa acagtcaaaa agaatgcttt
     5341 ttctaccacg gcagtcaaag atatcttacg caataaaatt tatgcagggt atttagaata
     5401 tgcgcgctac atcaattggg aaaccaaacg acggaagggg aagaatccga gaccgatttt
     5461 agtgaaagga acccatgaac cgattatttc ggaagaagat tatcaaaaag tacaagaacg
     5521 ccttgcttta gaaggaaaac aacccaaatg gaaccataca ggtgaaaatg tattgacagg
     5581 cttgttacgt tgtccagaat gtggcgcacc gatggcagcg agtaatgtca caaataccct
     5641 aaaggatggc acaaagaaac ggattcgata ttattcttgt tcagtctttc ggaataaagg
     5701 agcaagtgtg tgtcatgcca attcgattcg tgccgaacaa gcggaaacat ttgtcgccga
     5761 ccgattaaag gagatcgttc agctaccaga agttcttccc cgattggtgg cagcgttaaa
     5821 tgaagaaata gtgcgacaaa gccaaccatt ggaacaagaa ctcgtggtgt tactggaacg
     5881 aaaggaagaa ttgaagacta aaattgaaaa atgggaagct gctttggaag atagtcctga
     5941 attgtttccg atgttaaaag accgcttaga cgaattgact gaaaaacgac gacagttgca
     6001 cattcgagaa aatgaaatct tagggatctt tcaacaagga gagccgattc aagtcaaaga
     6061 tgtccaacgg atattgacta gtttggatcg ttttcttgcc cagagtgaga agaagcaaat
     6121 caaagccttg tatcgtacct ttatagagaa aataaccttt gatcctaacc ataaggagat
     6181 gttggaaatt acgatgaaat tcacgcatgc cgtggtacaa caattgaatg agcaatatca
     6241 aatagccgta tccaaaacaa aggatacggc tatttttgtg ttaaaaacac cctttacgtt
     6301 aacgatctag tctaggttat atcaaacaac gttagagggg cgctaacgct tctctagcga
     6361 tttgaaatcg ttagagaagt agttagggag gtccttatca taaataggga tatttattaa
     6421 tgaatatttg aaatataatt gtatataaga attaaaaaga gtggggatag atatgaaaaa
     6481 gatgggtaag tgtattttat gtgaaaaata cactgaactt actaaagaac atgtaccacc
     6541 taaaaaatca gggaatactg gaggtaaaaa gaccagaact ggtaatttag atgatttcct
     6601 taagagcgat tttacaaaag gtgattttcc gaaggggatt aaaaggaaac ctcaaggaaa
     6661 tgtttattat a
//
LOCUS       DACCYH010000121         6405 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.127, whole genome shotgun sequence.
ACCESSION   DACCYH010000121 DACCYH010000000
VERSION     DACCYH010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.127"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(99..1229)
                     /locus_tag="IVY85_002675"
     CDS             complement(99..1229)
                     /locus_tag="IVY85_002675"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP8045636.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IVY85_002676"
     CDS             complement(1878..3080)
                     /locus_tag="IVY85_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8045637.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IVY85_002677"
     CDS             complement(3140..3343)
                     /locus_tag="IVY85_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8045638.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IVY85_002678"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IVY85_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IVY85_002679"
     CDS             4880..5692
                     /locus_tag="IVY85_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8045639.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1919 a         1311 c         1007 g         2168 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgcc
//
LOCUS       DACCYH010000122         6287 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.128, whole genome shotgun sequence.
ACCESSION   DACCYH010000122 DACCYH010000000
VERSION     DACCYH010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.128"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..177
                     /locus_tag="IVY85_002680"
     CDS             <1..177
                     /locus_tag="IVY85_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045640.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="IVY85_002681"
     CDS             190..690
                     /locus_tag="IVY85_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8045641.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="IVY85_002682"
     CDS             787..2634
                     /locus_tag="IVY85_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAP8045642.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="IVY85_002683"
     CDS             2637..3533
                     /locus_tag="IVY85_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8045643.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="IVY85_002684"
     CDS             3583..3972
                     /locus_tag="IVY85_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8045644.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6287
                     /locus_tag="IVY85_002685"
     CDS             3959..>6287
                     /locus_tag="IVY85_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8045645.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacg
//
LOCUS       DACCYH010000123         6237 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.129, whole genome shotgun sequence.
ACCESSION   DACCYH010000123 DACCYH010000000
VERSION     DACCYH010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.129"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..2046
                     /locus_tag="IVY85_002686"
     CDS             <1..2046
                     /locus_tag="IVY85_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8045646.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVDLTDNVTNLPTLPEGT
                     TVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGE
                     KVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            2180..3523
                     /locus_tag="IVY85_002687"
     CDS             2180..3523
                     /locus_tag="IVY85_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8045647.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            3635..5086
                     /locus_tag="IVY85_002688"
     CDS             3635..5086
                     /locus_tag="IVY85_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8045648.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            5435..6067
                     /locus_tag="IVY85_002689"
     CDS             5435..6067
                     /locus_tag="IVY85_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP8045649.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT         2415 a          886 c         1232 g         1704 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aaggcgaaaa agtagaagtt
      421 ggaggcacag tagatctaac agataatgta accaacctac caacattacc agaaggaaca
      481 acagtaacag atgtcactcc tggtggtacg attgatacca acacaccagg taattacgaa
      541 ggggttatcg aagtaacgta tccagatggt acgaaagata cagtgaaagt tccagtagaa
      601 gtgacagaca atcgttccga tgccgataaa tatgaaccaa cagtagaagg cgaaaaagta
      661 gaagttgggg gcacagtaga tctaacagat aatgttacta acttgccagc gttaccagaa
      721 ggaacaacag taacagatgt tactcccggc ggtacaattg ataccaacac accaggtaat
      781 tacgaaggtg tcattgaagt aacgtatcca gacggtacga aagatacggt gaaagttcca
      841 gtagaagtga cagacaatcg ttctgacgcc gataaatatg aaccaacagt agaaggcgaa
      901 aaagtagaaa ttggaggcac agtagattta acagataatg ttactaactt gccagcgtta
      961 ccagaaggaa caacagtaac agatgttact cccggcggta caattgatac caacacacca
     1021 ggcaattatg aaggtgtcat tgaagtaacg tatccagacg gtacgaaaga tacggtgaaa
     1081 gttccagtag aagtgacaga caatcgttct aatgcagata aatacaatcc tgaaggacaa
     1141 aaagtgacta cagacttaaa taaagagcca gatgcatctg agggaattaa gaacaaagaa
     1201 gatttaccaa aaggaactac ttatgcttgg aaagagaaag tagatgttag cacagctgga
     1261 aataaaaaag gtactattgt agtgacatat ccagatggat catctgatga agttgaagta
     1321 gacgttacag tgacagatac tcgttccgat gcagataaat ataatcctga aggacaaaaa
     1381 gtgactacag acttaaataa agagccagat gcatctgagg gaattaagaa caaagaagat
     1441 ctaccaaaag gaactactta tacttggaaa gaaaaagtag atgttagtac agctggaaac
     1501 aaaaaaggta ctgtagtagt aacatatcca gatggatcta aagaagaagt agaagttact
     1561 atttctgtag tagataaaaa agcaccgaat aaacctcaag ttgatcctat tacagagggt
     1621 gaccaaattg ttactggtaa aactgaacca aatgcagagg tgacagttac attaccagac
     1681 ggaagtcaac accatgggac agctgataaa aatggtaatt ttacagttaa agttcctaaa
     1741 ttagaggcag ggacaaaagt gatagtaact gcaactgatg aatctggtaa tactagtgaa
     1801 cctactaatg tagttgtttc ttccaacaaa aaagacagtg gaaaaaacgg tagtaaaggt
     1861 agtaagactg acaatcaagg tagcaacagc aatcaagaca aaaatagagg caaaagccag
     1921 tcctcaaaag tttttcctaa gacgggagag agtgacagta atatctttac tattagcggt
     1981 ggtttaatac tgttaggaac gttaggatta ttagggtata aaaatcgaaa aaaagagaat
     2041 gaatagaaaa tcaatttctt aagtcttctc tgagaagagg aagaatcttt ttcttcctct
     2101 ttttgctttt ttatgatggg aaataagtgt tagttaaaga tgaatatact ttttatagaa
     2161 aggaaaagaa tgtaattaag tggaaaaaat agtaattatt ggttcaaacc attcagggct
     2221 agccgcagct cgatttctaa acttaagcga ttcaaattat gaagttattc taatcgataa
     2281 aaaatcagaa attagttact tggaatgtgg gacatcgtta tttataaaag gagaagtaag
     2341 taatttagaa gatctttttt atcaaaatag aatgacacta aattctgaga taaaaaagtt
     2401 atttctagag acagaagttg acaaaattga tattcaaaat aaaaagatta gttgtgtaaa
     2461 aaaagatggt acaagattct ctcaagaata tgacaaagtt atattagcta caggttcaaa
     2521 gcaaattatg ctagatatac cggggaataa tttagctaat attttttcga taaagaattt
     2581 tccaaatgca aaagagattt ttaaagcttt ggatgatagt actattaaaa aagtaggaat
     2641 tattggagca ggatatattg gagttgaaat tgctgaagcc ataagaaaaa gagggaaaga
     2701 agtatatctt ttcgatgttg cagatagagt gttgtcgact tattacgacc gtagtttttc
     2761 agataaagtt gaagaaatac tatcaaaaaa tggaatacat ctttgtctat cagaaagtcc
     2821 taaaaaatat ttaggaagaa aaaagattga aaaaatagtc actacaagtg gtacccaata
     2881 ctctatggat atggtcgtac aagctatagg atttctatct aatagtccaa ttggggaaaa
     2941 ggaactcttg agagatgtta gtggagcata tctaactaat gaataccagc aaacaaatat
     3001 aaaagatata tatgctattg gggattgtgc tacaactttt tttacgtcca ttaatagaag
     3061 tagtgttgat ttttctattt ctaatgctct aagaacagct tatattgcaa ctcaacatat
     3121 taacaatcaa gattttacac cttctggttc ccaatttaca aatggctttt ctatatttaa
     3181 ttacaatttt tacgcagcag gtttgaattt aaaaacagca cgattattaa gtgagtctat
     3241 agattatata gaaatagaag atttcattag acctgctttt ttgagaaaaa atagtcgtgt
     3301 aaaattaaga attagctttg aaaaaaatac aaagagaata gtaggtgtac aactttgtag
     3361 tcaagaagat ttatctggac tattatcaat gttttctctt gctatagaag aacaaattac
     3421 attagacaaa ttgaaaatgt tagattattt atttcatcct agttttagcc aaccctataa
     3481 ttttatttta aaggcagtaa caggattaaa cattgaaaca taatcgttac taaatgaaat
     3541 caagaaatat actgtattac tcaattcatt gaaaatattt atcataattt ttaatagaaa
     3601 acactattaa agagaaaaaa gagaggtaaa tcaaatgaaa aaatctttaa aacttgcatc
     3661 agcagttata ttaatgtccc aactccttat agcaacacct ttccatgtgt tggcaagcga
     3721 aaattcaaca tacaaaattg caaaagaaag aaccgagacg actgaaatga gtaattccga
     3781 tgtaacagca gagagtagtg aacttttgac aacttcatca agtgaaagta gtacgacttc
     3841 aaatgccgag tcttcaacaa cttcatcaag tgaaagtagt acgacttcaa atgccgagtc
     3901 ttcaacaact tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc
     3961 gtcaagtgaa agtagtacga tttcaagtac cgaatcttca acaacttcaa gtagtacaag
     4021 tgaaataact tcttcgaaag tgacaaaacc aaaccatata gaatcttcaa aaaaggaagc
     4081 gccaaaaacg gaaggacaaa caaaaacttt ttcgacacca cagcaaatag aatcaacaga
     4141 atctttccaa tcacaaccaa tactatctgg taatgaagaa attcattttg acaaaaatga
     4201 atctgtagaa aattttatta tgaaaattgg tgagtctgcg agaaaaattg gtcagaaata
     4261 tgatttatat gcttctgtta tgattgcaca agcgatctta gaatctgcca gtggtcaaag
     4321 ccagttagct caggcaccga attacaattt atttgggata aaaggaactt ataatggaaa
     4381 ctttgtcatt atggtaacaa acgaagattt gggtaatgga acgctttata cgacccaatc
     4441 aaaatttaga gtgtatgaaa actatgagga aagctttgaa gattatgcca aattattaac
     4501 taagggaatt agtggaaata aagattttta tgcaggagca ttaaaagcta atagtaaaac
     4561 atatcgagaa gcaactaaat tccttacagg acggtatgca acagatacac aatactattt
     4621 gaagctaaat gaattaatta aaacctacga tttaaccaac tatgataaag aagttaaagg
     4681 tccacaaatg agtaagactg gataccaagt gcctattcat aattattcaa taaccagttt
     4741 gtttggaaat cgtggaaatg aatttcaccg aggacttgat atggcagcta cacagggtga
     4801 gccgattcat gcagttaaag ctggtaaggt tgttaaagca gagtttcact actcttgggg
     4861 aaattacgtg gtgatacaac acgaagatgg atcaaccgcg ttatatgctc accagcaaca
     4921 atacctagta aaaactggag ataatgtttt gcaaggacaa ataattggct atgtaggaag
     4981 tactgggaat agtactggcc cacaccttca tttggaaatt tgcaaagata gtagtctttc
     5041 tcagagcatg ttagttgatc cgaaaacctt attattcgga gactaaaaaa ggtaatcaat
     5101 ctgacaaagt aacaagaaaa tggttggatt cttagacctt tgatcaggat attgagactg
     5161 gaattgatat ctcgattacg atgggtttga caaataaaac aagtccatta tcgaatatcg
     5221 agtgtattat tgccgtcttt atgctcatat tttgagcagg tttcactaga tgaaaacaat
     5281 ttatctatct tgaggatttc gtgtacgtat cgttcagtct tgtattgtaa attagtcata
     5341 aaatcagact aagaaaaatt ggaaatattt ttttctgatt tattaaaagt taattgaaat
     5401 tatatggtac ttctttcttg ggagggtagg tgcagtgaag gaaagatatg gtcaccttac
     5461 tgttgttgag gagattactc ataatggata taaaaaatat cgttgcctat gtgactgtgg
     5521 gaatgaaacg attgtatatc gatcaaactt actatcaggt agaaccaaaa gttgtggatg
     5581 tggaaattat aaaaataggc ataaatataa aaatttgaca aataaaactt ttggtaagtt
     5641 agttgcaatc aagccaacta aactgcgaag tgaaggaact gttgtttggg aatgtcaatg
     5701 tctgtgtggg agaaaaaagt tagtatcggc aaagcgactt ctaaaaggtg atgtaaaaag
     5761 ctgtggatgt ttgaaatcaa aaaagcatca cattgaaaat aaaaaattcg gattacttac
     5821 tgcaaagtat cctttggaca ataactgttc atggaaaaca aaatgggttt gtcattgtga
     5881 gtgtggcagt atgtgtgaga taagttatag taacttattt tttggacata ctcaaagttg
     5941 tggatgttta aaaaggcaag aaacaaggac atgtgttgaa gggacagtaa tagagtgtct
     6001 aacgtcaaaa ttaccgaaaa ataatacaag tggtgttaag ggagtttatc gttctaaaga
     6061 gaaatagatt gcgtatataa ctttaaaaaa aaagaggtac tatttaggat cgtatgattc
     6121 tattaagagt gcagctcaag ctagaaaaga tgctgaaaat aaattgtttc aacctttgat
     6181 tgacaaatat cgaggtaatc attaattatt gtggataata attaacagtg atatata
//
LOCUS       DACCYH010000124         6206 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.130, whole genome shotgun sequence.
ACCESSION   DACCYH010000124 DACCYH010000000
VERSION     DACCYH010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.130"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            149..994
                     /locus_tag="IVY85_002690"
     CDS             149..994
                     /locus_tag="IVY85_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8045650.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1275..2495
                     /locus_tag="IVY85_002691"
     CDS             1275..2495
                     /locus_tag="IVY85_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP8045651.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2492..5212
                     /locus_tag="IVY85_002692"
     CDS             2492..5212
                     /locus_tag="IVY85_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8045652.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5196..6140
                     /locus_tag="IVY85_002693"
     CDS             5196..6140
                     /locus_tag="IVY85_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8045653.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2318 a          970 c         1206 g         1712 t
ORIGIN      
        1 tcaaaaatac agagaatagt atataaatgt cctttcctat tcttaaaatg atgtttgtac
       61 ttacttaaat taggtataat tagtatatgt tttgtgagaa atttagactt acaataagca
      121 gtgaatttat aatcaacgta taggagacat ggatgaaatg aaactaattg gtatagattt
      181 agacggtact cttttgaatt ctgaccagag cattagtcaa aaaaatgcaa gaacaatgaa
      241 acatttaccg gaagactgtt ttccattcat ttgttctgga agagaagtag aagacattaa
      301 taatattctc aaaaaatctg gtttaaggat accagcagta gggttaaatg gttctgttgg
      361 atttgatgga gaaaaaaaga tatttgagtc ttccttcgat aaaaatgatg taaaaaaaat
      421 tgattctgtc ctatcgaaat ttccaaccaa aatatataca aatctcggaa gttatgaatc
      481 tcagaattat aaaaataaaa tgaaaaaaat ttttcacgag gtagggagtg aattttctat
      541 caaagagcta aactatgagc ttgagtatga aaaatctatt tgttcaatcc cttataataa
      601 tattgaggaa attatagaaa aggatgctat cagcatttac aaatttttta tttttattcc
      661 taatcgtcaa ttgaaagcta atatatttaa ccatttgaaa aagatgctaa atgtgtctgt
      721 tactgaatcg gcacctgtaa atattgaaat cgtacctcaa aatgtatcta aaggtgtggt
      781 gtttgatcgt ttggaatcaa tctacaattt aaagaaacct ttgagaatcg cgatcggaga
      841 tagtttaaat gacttatcta tgtttgaaag tgcagatatt agttttgcaa tgggaaatag
      901 tcatcaagaa atcatgaaaa aagctgatta tgtcactgtt tcaaatgata aagacggagt
      961 atctcaagca tttgaaagaa ttcttaccat ataaactaat taaggtagta tagaacatag
     1021 aattctaaat acggaaaaat ggagcataaa ttccagtaca aatagtactt cttcagtgtc
     1081 aaacgaagcg aatataacat agatatttag gattgtgaca aaaatcatgt cacaatcttt
     1141 ttttcgaata aacttatttt ttgaagtcgg ccatttatcg taatttcttt gcataatacg
     1201 aacaaatttt cggttattga tgaaaaaaaa cattgaaaat ggtatgataa aagaaaaaaa
     1261 ggaggaaatt tttaatggtg aaatttatcc atgcggcaga tttacatatg gatcggtcat
     1321 ttgaaggact gacaactctt gataaaactg tacaggaaaa actattgaag acgaatctga
     1381 ccgttttatc aaatatcatt gaacaggcaa taataaataa cgttgatttt gttttacttg
     1441 caggagataa ttttcatcaa aatcgtcctt cattaaaaat acagaaacat ttttcagaac
     1501 agatgaaacg gttagaaaag aaccatatac ctgtatttat aatattcgga aatcatgatt
     1561 tctatcaaaa agagcggtat tggttctcct ttcctaaaaa tgtccattta tttacatccg
     1621 aaacggtaga gacaaaaaaa ataacgataa aaagtggaga aactgtctct ctcagtggat
     1681 ttagttatcg tcaaccgtgg attcaaaaag acaaagtgat ggaatttcct tctagagaac
     1741 tcacagatta ccatattggg ttgtaccacg gggaacctgg cgtctctcaa aaaggaaact
     1801 atgctccttt ccaaccctca aagatgcaag aaaagggata cgattattgg gctttagggc
     1861 atatccatgt gccaacggtc ttaaatgaaa agcaaacaat tgtttatcca ggtgcgcctc
     1921 aaggtcatac taaaaaggaa caagcttcta catctattct actagttgaa ttaagtaaag
     1981 gaagttgcca aatacatccg attaaagtag cagaggtcta ttggaagaca aaggaaatat
     2041 cattgaggaa agcaagaacg acaaaagagg tcattgttca cattcgtcag cagctttcaa
     2101 aagtagagga tggatttagt ttaattgaga taaaattaaa agattatgac catctcaata
     2161 cagatgtttt ggaacggata caatcaggag agctattaga ctatttgtta gaggaattct
     2221 cagataggat aaatgatttc tttatttggc gtatttctct catagagaat actttttata
     2281 cgaagacccc acttgctgct tctgctaaat tgatggaaca gttatttcaa tactatcaga
     2341 cacctcaaga tttccaacaa attttaaatg aagtatatag tcatcaagaa gcggctagaa
     2401 tattgagcga actgccggaa tatcaagaag aaacacttga aaaagccaag cagctgctta
     2461 atcaagattt tttatttgag gaggatcagg aatgaagatt ttaaggatcg agattgctgc
     2521 ttttggaaaa tggcgtcaaa aaagttttga cttttattca ggcaatcagc tgatctatgg
     2581 gggaaacgaa gcaggaaaat caacgattta tcaatttatt caagcaatat tatttggttt
     2641 cccttcaaaa ggccgtaaaa aaaaggatta tacaccaaaa gacggctcag cctatggcgg
     2701 caaaatatgg cttaaacatc ctgtatatgg tgaatttgct gtcgagcgct acaagcagca
     2761 aaatcgcgga aaatcgaagg tgtggctggg ggatcaagtg ggaagtgatg agttactaga
     2821 aaaactgatc gcacctttga cgaaagagtt attccagcag gtatttactt tccagcaaga
     2881 gcagttgatg gaactagatc atttgagaga aaaagagcta catgatgcgc tgatttcttt
     2941 aggcatcacg ggcagttctc aagtattcca aaagcggcaa gaatattatc agaaagcaca
     3001 acaattattc aagaaaaaag gccaaaaatt actaatcaat caaaaattaa atgaatggaa
     3061 agagctgcaa gaaaaaattc atcaaaaaca agaacaggaa gcttattttg gcaagttgat
     3121 ccaacaagaa caggaatacg agtcgcaaca gcagcagtta ttggcacaat taaaagaggc
     3181 gaacaaacaa ttttcattag ccagacagca agaacttaat ttttctttat atgaagaatg
     3241 gcaatctctc aaaaaaaaca gcactacagg tcttcctgat gagcaacttc ttttagatct
     3301 ggagtctttc tccaaacggt atcagcaaac aaatgagcgt ttggaagaat tagaagcaac
     3361 actggaaaag catagcggga tggatcagca gtcagccaga tattattttt atttagagca
     3421 ggagcaggaa ctaaaagatc tccagcaact tcaatttaca gcggaacaat taaacgaaga
     3481 atggcaaaga ttacaaattg agactagaga aacaaattca gaattgaaga ttttggaaca
     3541 aaggtggcat tggcagaaaa atccgttacc ggaagaattt ttatatgaag aagaatggaa
     3601 gcatcttctg gctgaagaaa gaaggattgc tgatctagaa agccagattt ctgtgcgtct
     3661 ccaaatagct aaagaacagc aagagatgat tgaagcagaa gtgaatgact tggaagagac
     3721 ctatccagca ttattacaaa atcagtcaga gatagcacca gtctcttcat ctaaaccgta
     3781 tatgctgatg gctggcggag caataatcat tgcaggtata tttgtaccaa tgcccttgaa
     3841 agtgatcctt ttactaattg gtgcaggcat gattggctat ttcttctaca aaaggcaacc
     3901 aaaaattaaa actaaaaatg atttctctga gataaaagga atgtggcaag aaaaactagg
     3961 acaacttgat ctagccttag ctcaattagc tgaagcagaa gaaaaacaaa agagaactgg
     4021 agaagaaaaa cagattcttc agcaagaagt atatcgtttt gcggatcatc ggcacttagg
     4081 aaagatggat acgctcaaac tcatgaaaga atatggacag gaagtcatag attatcagaa
     4141 attatatggc gtatttgagc ggaaacataa aagagaacag gaaatacaag aaaaactacg
     4201 acaaatcgac caaaaatttg agtttgttaa agaatggtta ccgatacaag ataagacaat
     4261 ttcagaaaaa atggtattgc tttcccgttt tatacaagag atggaacaga tgaaatttgc
     4321 cagaagctat cagcaaaata ctttattgaa gcaagaaatc aatcaattga aaaagaaaca
     4381 aaaagtatgg atacaagaat acagcgaaca attgactcaa gcaggcttat cttatccttc
     4441 ggaagtacct gtctttttac aaaaagccaa acaagccgaa cagcagatga aaagatacaa
     4501 tgaattatcg gaaatgctta ctccactatt tcctgaagag ctgacaaaag agaaattatc
     4561 tgtaagactg cgccaactga aagaaaagca atcccaactc cagcaagaag aaaaagaagc
     4621 aagtgaaaat cggcaacgac tccaacttca agtagaagag ttgcaaagag acggcacatt
     4681 ggacgaactt tatcaacaag ctagccggca aaaagcaaaa ttacaggaat tactggagga
     4741 atggggagca cttgaaatcg ccgggacatt cttaggtgat ctttcaacag agatgagtga
     4801 aagacaatta ccccagttgt taagagcagc aggtgattat ttaggtattt taacaaaagg
     4861 aaattatcag aagatattac tgaaaaatga tacactgact ttaacagacg gatatacaga
     4921 atttcccatc tatgagttat ctacaggtac gaaagatcag ctgattatgg cgatgagatt
     4981 tgcttacctc tcattagaag ctaaccgttc gatatgcccg atcatcattg atgatggctg
     5041 gcttcattat gatcatcagc gaaagtatca attagctgaa cttttagcta aatttggaga
     5101 aaagtatcaa ataatttgtt tttcttctga taaagaaatg gtaagctatt accatgagat
     5161 gaaccaaatc gtaaaacaat tagaaggagc aaataatgaa aaaaattcgt gatttagtaa
     5221 tcgatgagca gtttgaacaa tttgtcctga tcaaaagtgc agatgtaaga atcgctaaaa
     5281 acggaaaaaa atttattgct tttactttcc aagatacttc aggtactatc gatggtaaat
     5341 actgggatgc ttcggaggag gaaatcaata aatttacagc cggcacagtc gtttttctaa
     5401 acggtaaaag agaggtttat caaggaaacc ctcaagtaaa gattcttcat atgcgcttaa
     5461 caagggtaga tgagccagga gagcctagtt tatacatgga aagagcccct ttgaagaaag
     5521 aagaaatgga agaagaaatc aatcaattgt tgtttgagat caccaatgcg cattggaatc
     5581 gtattgttcg ctatctactt aataaatatc aaaaacaatt ttttgagttt ccggcagcaa
     5641 aaagaaacca tcatgcattt gccagtggat tagcttatca tactttgaca atgcttcact
     5701 tagggaaatc aatcgccgat gaatatcctg agctgaatcg agcattgttg tatgctggta
     5761 tcattttgca tgatttagga aaggtgattg aactttccgg tcctatgtcg acagaatata
     5821 cgcttgcagg aaatttgatc ggtcatctgg tattgattga cgaagaaata actaaagcct
     5881 gtcttgcgtt gaaaatcagc gaagctgacg aagatgttct cgtattacgt catatggttt
     5941 tagcacacca tggcttatta gaatatggct cacctgtacg tccaagaata atggaagcag
     6001 aaatattaca tcaaatcgac aatatcgatg cttcgatgca gatgatgttg acttcgattc
     6061 gtcaaacaga accaggaaaa tacaccgatc ggattttcgg tatggacaac cgaagtttct
     6121 atgtaccaaa agatttatag caaaaattta tttttttcat tcaaaacaaa aaaagcgaga
     6181 cataagtctt cgcttttttt gttttc
//
LOCUS       DACCYH010000125         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.131, whole genome shotgun sequence.
ACCESSION   DACCYH010000125 DACCYH010000000
VERSION     DACCYH010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.131"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            725..811
                     /locus_tag="IVY85_002694"
     CDS             725..811
                     /locus_tag="IVY85_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP8045654.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IVY85_002695"
     CDS             complement(932..1621)
                     /locus_tag="IVY85_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045655.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="IVY85_002696"
     CDS             complement(1866..2780)
                     /locus_tag="IVY85_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8045656.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IVY85_002697"
     CDS             complement(2762..3142)
                     /locus_tag="IVY85_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP8045657.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IVY85_002698"
     CDS             complement(3592..3879)
                     /locus_tag="IVY85_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8045658.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IVY85_002699"
     CDS             complement(3899..4123)
                     /locus_tag="IVY85_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP8045659.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IVY85_002700"
     CDS             complement(4763..5296)
                     /locus_tag="IVY85_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8045660.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IVY85_002701"
     CDS             complement(5317..6159)
                     /locus_tag="IVY85_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP8045661.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACCYH010000126         6072 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.132, whole genome shotgun sequence.
ACCESSION   DACCYH010000126 DACCYH010000000
VERSION     DACCYH010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.132"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(76..606)
                     /locus_tag="IVY85_002702"
     CDS             complement(76..606)
                     /locus_tag="IVY85_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8045662.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(788..1444)
                     /locus_tag="IVY85_002703"
     CDS             complement(788..1444)
                     /locus_tag="IVY85_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP8045663.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(1571..2044)
                     /gene="dfrF"
                     /locus_tag="IVY85_002704"
     CDS             complement(1571..2044)
                     /gene="dfrF"
                     /locus_tag="IVY85_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAP8045664.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(2439..3830)
                     /gene="amt"
                     /locus_tag="IVY85_002705"
     CDS             complement(2439..3830)
                     /gene="amt"
                     /locus_tag="IVY85_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP8045665.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGFLAMTISMVVSLYEYPTFATSGFSLVFFLLLGGLLWFIPVALCAAEMATV
                     KGWEKGVSQTKRY"
     gene            complement(4171..4413)
                     /locus_tag="IVY85_002706"
     CDS             complement(4171..4413)
                     /locus_tag="IVY85_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8045666.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(4445..5002)
                     /locus_tag="IVY85_002707"
     CDS             complement(4445..5002)
                     /locus_tag="IVY85_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8045667.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(5015..5203)
                     /locus_tag="IVY85_002708"
     CDS             complement(5015..5203)
                     /locus_tag="IVY85_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8045668.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(5216..5782)
                     /gene="amaP"
                     /locus_tag="IVY85_002709"
     CDS             complement(5216..5782)
                     /gene="amaP"
                     /locus_tag="IVY85_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8045669.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         1828 a         1276 c          892 g         2076 t
ORIGIN      
        1 aaaaatctta aggattttcc cgccaacgtg gtcggatcgc tccgtcggtt taacagcttt
       61 tctgtttctc tctagttatc tacgcatata aatcaaatcg gttattccgt tataagtctg
      121 tgttttacaa agccttaact ttatttccct gtctatgttg cccaaaagac gaaccccatt
      181 tcctaacaga gtaggaataa cagaaatgta atatttatca atcatatttt tgcttaccaa
      241 ctgctgaaca agatttgctc cgccacaaat ccaaatatcc tttcctgctt cttgttttag
      301 ccgttccaat aataaaacag gattttcact tgtaaaatga atttgttggg aagaattgca
      361 ctctttatgt gttaatacat aagttgtaaa tttattatat acccattctt gcggagaaag
      421 ttcagtaaca acctgatgag aagtattcca ccccattaag acagtatcta tatctttggt
      481 aaattctgta taagtgtcga tgttttcatt gtcatttcca tgaccattta gccaagctac
      541 tccaccatta ccgtccgcaa tatatccgtc aaggctcatg gcaataaata aaactacttt
      601 tctcattttt tctcttttat aaatacaaac aggaatttat ttctcttttt tactcataag
      661 ggaacaactg attgctacaa ataaacccat taaaatccat ggaaatgctg ctcttataaa
      721 atcactaacc atactttatt cctccttaaa ttccaatttc cttagttcta caaactgaaa
      781 tttttgccta tgaatgtatc ttgttcttta tacgcaaaca ggcttctgtg tgcataatcc
      841 aatgtcttga aaacggcatt tgaattagtc cacaaatatc tttattacac caataatcaa
      901 tcagccaaat tgctttttca ttgtcgttta ctacattcaa cgattctaag tatccttttc
      961 tttctttcgg tatttttctt ttcaattcat cataagataa ccgttcaagc attttctcag
     1021 tgctttccca tacttcgaaa atatataaat ataattcttc cagattaagc tgttttgaaa
     1081 agtctgcaat ctgctgtttc atgagttcat ttcctgttgt aatgataggt gaattgatac
     1141 gctcttgata attgcctgca aaaaatactt gttcatcttc atttatcact gtatgcacaa
     1201 ctatatcttc aatgcggaaa atatgccaaa tagaataagc aatcgtctta ctatgataac
     1261 catcagcgtt tatgaagggg attgagttaa aatcttctct gcataattct tctttgaaag
     1321 atactaaggt ttgtattaac tgacttcgta aagtaagtaa agtatcaata cccctcttat
     1381 aagtatcttt cttttttatt tgtgcctgca ttgttttatt aagttcagac cattccttat
     1441 tcatattcaa tcctctctca aattctgatt ttatcgcccc atacaaactg gaatttgtct
     1501 tacaacaatt tatatatgca taaaaagcaa tatatttatg aaaacaccct aaatccaaaa
     1561 tttactcaat tcattttttc cttacataaa atgttctcgt atatttcact tcctcaccaa
     1621 gtgtttcccc tatcaaaact tcaaaatcat tgatatcaaa ttctggaaag aatgtatctc
     1681 catcttcaat atttaaatct acttctgtga tatacatttt atctactatt tgcaaagctt
     1741 ccttaaatag tccatatcca ccagatatgt atacatctcg tcctttagcc aataataatg
     1801 catcttctaa tgatttaact gaaactaaat tatctccttg atactctgtt gtggtggaaa
     1861 caacaatatt cattctatta ggcaacggat gaccgatttc ttcataagac tttcgcccca
     1921 taataaccac attacccgtt gttaactctc taaattgctt ttgttctccc tttattttcc
     1981 atggtatatt accattcttg cctataacat tattctttga cctcgcaaca atcaaaccta
     2041 tcatctattt catcctccat aatatgatgt cgtacctgtg attccatcaa gtgcaccaca
     2101 tcttaacaaa ctcaaaatta tctcgaacta accagctcga caaattcctt ttttgtcttc
     2161 atctgcatta tatcatattg ttataagcat tgccatttct tttctgcttt tgaaaagtta
     2221 cttatcagcc tatgttttgt aaagggtgat attcagcatt tctcacgcaa caagcccttg
     2281 acaaaccata gactaataag ttttaaggta atcaagcaga aatgaaatag tctgctgttt
     2341 cacggcaaca aaaaagagat accaacacac ttatgtagta tctcttagtc tagtagcaat
     2401 attcttttgt ttgcaagcag gtaaacatgg gctgtttctt aatatctttt tgtttgggaa
     2461 acaccttttt cccatccttt caccgttgcc atctctgctg cgcacaatgc cacagggata
     2521 aaccatagca aaccgcctaa taacaagaaa aatactaagc tgaagcctga ggtcgcgaat
     2581 gtgggatatt catataatga aacgaccatt gagatggtca tagccaaaaa gccaaagaag
     2641 aaaaagtcca atccttcttc ttgttcttct tttgtagtag tgatcgttgt aaagtgcatc
     2701 gccgatttta aaatcactgt tgtcagcaca taggcataca cggcagtaat gatgacacca
     2761 atcacttgaa tccctaagaa gtaccagctg cttggattcg ttagtaaatg gctgttagat
     2821 gcaaagatcc ctatcagaat cgtacctgtg aatccgccca tgccatgaac accccacaca
     2881 tcaagtgcat catcccattg tttcttttta cgaaaatcaa cagcagcatg acagacgata
     2941 cccgcaatga tcccaatcag catggcactt tttgcttcta cataaccggc acaaggcgta
     3001 atggctgcca atccagcaac ggatcctgtt agtacgtcga caaaatcaaa gccatttcct
     3061 tttacttttg caatgatcag ccaagtaatc atcgcaaatg caagagcgat gaatgttgaa
     3121 ccaaatgcat tgactgcttg ttcatctgct cgaagtgcac caccggaatt aaaaccgaac
     3181 cagccaaacc aaagcagtcc tgtaccgata gcagctgcca taagactgct atgctgactt
     3241 ctttctccta aaggaatctt tctttgacca agtgtgagga tacatgctaa agagccgaat
     3301 ccagctgtcg tgtggatgac tgctccacca gcgaaatctc taaatcccat ctgatccaaa
     3361 aatccgccac cccatatcca gtggcaaaca gggaaataaa tcagcagatt ccaaaaaata
     3421 acaagtataa tatagccttt taaattcaat ctccctgcaa aagctcctgt catcaaagga
     3481 acggtaatca cgataaacat cagttggtaa agaaagaaca ttaaaaatgg aatcgttgca
     3541 ccatgaaaca tgttggggaa gcttgtaaca tgtctcatca aaaagaaatc tgccgggttt
     3601 ccaataacac cgccgatatc ccgtccaaag gccaatccga atccaccaaa tacccacata
     3661 atcgttgtta tcccaacgga gagaaaagat tgagacatga tcgttagtat attattcttc
     3721 cgtgctaatc cgccgtaaaa aaaagctaga ccaggagtca tcaggcaaac cattgctgta
     3781 cataaaatca tgaatgctgt atctcctgta ttgataggta atgtattcat ttttttcgcc
     3841 tcctttctac gattacgagt ataaaagaag gtgtagttgt ttttttcttc tgtttttttg
     3901 aattaatact tgcatttata aaagacagac aattaataaa agcttgatgt gttgtgctct
     3961 ttttcgtttt tcacacaaat aaagacaaaa aataaacggt cttcttttta tagaaaaccg
     4021 tttattttcg tcttttttta tttttaaaga tattttttca taaggaataa agaatacttc
     4081 atataaaata tataaaaaag aaccgcagcg agtgtcgcta cggttctcaa atttgttctt
     4141 catttcaaca taagtttttc ttcaatttgc ttataattta cgtgcaaaga atgaaacgac
     4201 taatactaaa atgattgcac cgatcaatga tggaatgata gccattcctg caattgttgg
     4261 tccccaagtt cctagtaaag cttgtccaat tgctgaacct actaaaccag caatgatgtt
     4321 tgcgatgatc cccattgatg atcctttatt cgtgattgct cctgcaattg ctccaatgat
     4381 accgccgacg attaatgccc aaataaatcc cattttaaat tcctcctaac tgacttctta
     4441 cttcttattg tacacgtggt tcgttatttt ctttgacttt ttgtgcacct ttgcctacag
     4501 cagattttgc tttatctgtt tggcgtgaag caaattgtcc tgtggatttt gctgcttctg
     4561 ttactttgtc ttgtactgtt tcttgatctt tttggtattc ttcttgtgtt ttgatgtctg
     4621 caacattcac attcacttcg ataacatcta aatgtgtcat attgtttact tcagtactga
     4681 tcaattcttt gatttggttg taaatatctt cgatgtcttt accgtattct acgatcacat
     4741 ctagatccac agctacttgt ttcttaccta cttctgtatt gatacctgct gttacgttat
     4801 ctgtgttgac taatttttca gccatatttg agaaaaatcc gccatctaat gtcaatagac
     4861 catctacatc tttcaatgca taaccgatga ttttttggat gactttgtct tcataagtca
     4921 attcaccatt catcatgcgg ttttctcctt tttcatttgc caaaactgat ccatttgttg
     4981 tattcatgtt tttgttttcc atctttgttt cctcctatga tttaaaaaat tgatcaacta
     5041 ttcctgactg tttcaaataa aaaccgattc ctgcgccaat tgctgtcatg attatgatca
     5101 acaatgtctt gaaaaatcca atcgttaacc ataaaacagc tagcagtagt ccaagacccc
     5161 caaagataat agggagcttg tatgctgaaa atagttcttt cattatttgg ccctcctatt
     5221 ccactcgaga gtgcttgtag tctcttgtat cttcttgttc ttcataaccg ctgtatgtga
     5281 ctgccacttt gaccgtttct tttgttccga gtactttagt tacttcttct tcgatatctt
     5341 gcatcaatac acctgttttc cctactaatg aagaagttcg tttcaagtct ccgttgacat
     5401 ggatatggat tcgatttttc gttgaacgta tccgtacttt aggtgaatga atgaactctt
     5461 cttcgtgtaa atgggaacgt accaaccctt cgatcgcttt tttgtcgatt gttagtttgc
     5521 catcttcccg tttcataacg aaagttcctc ttgactttgg atagaaaaga acgactagca
     5581 agatgataat agcaagaacg gcaaatacta cagctgccca gaaaatatat tgttgcatgt
     5641 agtaattcgt taaagtaaag aagcgaaatc gttccaattg ttcaggcata atatacggcg
     5701 cattgatcaa cgcaatcgct agaaatactg aaagtagcag taaactcatg ataacaccga
     5761 ttatttttaa ccccttattc attttccacc tcctataaac ttatttttac cctgtaaata
     5821 actctaccga caatcacagt atagatagcg aacagttagc atagcatcag taaataaagc
     5881 gctaccaaac attgctggat cataacggcg ttttattgcc agtctgatac tgaaataaca
     5941 attgcttact tattctctat ttacttttga ttgctggcta tacttaaagg atattattca
     6001 agattaaaaa ttgcaaagat tatgctcagt tattattaca agattctatt tatttattaa
     6061 attttttcgg ag
//
LOCUS       DACCYH010000127         6029 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.133, whole genome shotgun sequence.
ACCESSION   DACCYH010000127 DACCYH010000000
VERSION     DACCYH010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.133"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(68..370)
                     /locus_tag="IVY85_002710"
     CDS             complement(68..370)
                     /locus_tag="IVY85_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045670.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(590..956)
                     /gene="ssrA"
                     /locus_tag="IVY85_002711"
     tmRNA           complement(590..956)
                     /gene="ssrA"
                     /locus_tag="IVY85_002711"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1127..1900)
                     /locus_tag="IVY85_002712"
     CDS             complement(1127..1900)
                     /locus_tag="IVY85_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8045671.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2044..2394
                     /locus_tag="IVY85_002713"
     CDS             2044..2394
                     /locus_tag="IVY85_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP8045672.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2375..3091
                     /locus_tag="IVY85_002714"
     CDS             2375..3091
                     /locus_tag="IVY85_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8045673.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3091..4539
                     /locus_tag="IVY85_002715"
     CDS             3091..4539
                     /locus_tag="IVY85_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8045674.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4610..5119)
                     /locus_tag="IVY85_002716"
     CDS             complement(4610..5119)
                     /locus_tag="IVY85_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045675.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5109..5834)
                     /locus_tag="IVY85_002717"
     CDS             complement(5109..5834)
                     /locus_tag="IVY85_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045676.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         2052 a         1050 c         1170 g         1757 t
ORIGIN      
        1 aaaaatatcc cttttataag tatggtgata aaatccgttc cgggctacct tttttaggta
       61 gcctacttta atctttatac ctttctggat caacgaaagt atacttcaaa tagtcataac
      121 gccgatgatc gctccgtgcg tctggcacat cagtcacgat atcaaacaaa aaatacacat
      181 ctttcttcat tctggttttt gttgccggaa ttttaaagta gttcttatta gagtaataga
      241 gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaatcc cacaccttat
      301 aaaaatcttt gacaaatcta ttcgaaggat caaatttaaa ccataattgt gtttttcctt
      361 ccatcaacat aatgttcacc tcaaaaagag tatacgaact aatgttcttt ttgtaaagta
      421 ggaatatcat tgataatatg gatggaacat atatattaaa aagtgttctt tttgaatgct
      481 attttgaatg ccattttcat tcatttaacg aaaatctgcg aaaataaaaa atgctcaaac
      541 ccttataaaa taaggattta agcatctagc gaaatctgct gaaatctcac aatggagacg
      601 gcgggagtcg aacccgcgtc caaacacatc gccacttaaa aatctacgtt catagtcaac
      661 tcatttaagg attcacttta cagcttgccg agtgacaggc attctgtatc gctagtctga
      721 tgttctcttt ttcaacttac agacggaaag ttgaaacgta tcccactaaa gttaggaccc
      781 ttactcgagc acatgggcga tgccgagagg atctacgcta actgttttta ggcagctaaa
      841 gcgtaagagt tgttttcgtt tttagcagtt atatttaact gtaacgtttt tacgtagacg
      901 taacctacga aacgcaattc aagctcgact gtgcctgtcg aatccgtaac gtccccgtta
      961 gctaacaagg gttacgtgta tttttcatac aaaattagta tagcatatat tgggataaat
     1021 tcaataaaaa agcgaatcat atcccattat ttatataata actgcccctt tcttaagaaa
     1081 aaataagcga ttttcttaag aaaagaggta gcttcaaaaa aagatcttaa atcagatcaa
     1141 aatacttcaa tgcgtggaca attccgcctt ctgtattttt ctttgtgata aaggtagaaa
     1201 gttcttttaa tccgtcatag gcgttgccca tagcgatttt gttgtcgcaa gcttccaaca
     1261 aggcaaaatc attcggacca tcgccaaatc caaaagtagg aactttttcc agctgaagtt
     1321 ctttttgcag acgtttgaca cctgcccctt ttgagattcc tttcttcact gtatcgatcg
     1381 agtaaggacc attccgataa aaagttagct caggaaaatg ttcgtgatag tattcgtcac
     1441 ctgattgaga taagatcagc aacatattta ctgtgttttc tttaaagcct aaaggatcaa
     1501 tttccggaac atctgaatga atgaagtcgt aagcattgat cattgtttgg ctatgaccgg
     1561 tacaccagat tttctgatcg ttatagtaag aaagttcatg accgttcaac tgtacatgat
     1621 catacatttt ttgacattct tctgaagaaa atgtttgaga atagatttct tcgccgtcta
     1681 ctcgaatgaa agaaccattc atggtgaccg cagaagtaat gcccgcatct tccatgatag
     1741 agtggatttc tgcttctgta cgtcctgtag cgatgacggg gagaacattg ttttctttta
     1801 atgcgttcat agcttttgcc acttcgggtg tgatttttga tttcgcatct agtaatgttc
     1861 catcaagatc aaagaaagtg attgcacgat aatttgacac aaataaaact ccttttttat
     1921 tggaataagt ttatcttaac aaagaatgaa caaaagacaa acctttcgtg aagaattcct
     1981 gaacatattg gttcagtcaa caatttagag tatactataa gaagagaatt taggagggaa
     2041 agaatgattt ttttcagtat tggtatctta ttcttaataa ttggcagtct ttttcggatt
     2101 ctgccctcaa aaggaaatct gcctttttat gggtatcatt cgcctcttgc cgcaaaaaca
     2161 gacgctcatt ggcggctggc tcaaaagaca agcggaaact ggttttttct tatgggctta
     2221 ctgatggcat tgatcggcta ttatttaaag acatctgggc atacgaatta ttttctgatt
     2281 gaaatgcttc tgctagtatt tccaattatg cctattttta ttatgacaga aaagaaatta
     2341 cagaaatatg acctggaaac tggagggaac gataatgaat attttaatga ttgaagataa
     2401 tcaatctgtt tctgaaatga tgcagatgtt tttcttaaat gaaggttggg aagcaacttt
     2461 caagtatgat ggaaaagaag ggctcgatgc ctttttagaa aacccacaac attgggatat
     2521 gattacacta gacctgaatt tgcccacact ggacggtatg ctagtaggaa gagagatccg
     2581 caaagtttca agtactgtgc cgattatcat gctgacagct agagattctg aaagcgatca
     2641 ggtgatcggc ttagagatgg gggcagatga ttatgtcaca aaacctttca gtcctctgac
     2701 gcttattgct cgaatgaaag ctttgcatcg ccgtagtgag ttggcagaag caagagaaga
     2761 agcagtagtt tcagatgatc attttgatat cgagactagt catttcaaga tgaatacgaa
     2821 aacacgagaa gcgtatctgg acaaccggcc aattgaagga ctgacgccta aagagttcga
     2881 tctattgttt actttagcaa aaaaaccacg tcaagtattc tctcgggagc agttgcttga
     2941 attagtctgg gattatcagt actttggtga tgaacggaca gtggacgccc atatcaagaa
     3001 attacgacaa aaaatcgaaa aggtcggacc acaagtcatc caaactgttt ggggagtcgg
     3061 ctacaaattc gatgattctg gtgttgccta atgcgttatt tgtatcagca actcttagct
     3121 ttctgcggaa tggtcgcgtt gatcttattg atcgttggag tttcttttac acaactgaca
     3181 aaacaaacga ttgaagaaaa caattaccag caactgtttg gatatgctga atcagttgaa
     3241 aagacaacgc aaacctatgc cgatacgctc ccgcagttta gtcaagatca ggcgtttcaa
     3301 aatgcattgc tgctgacaga gcaagtactg acagaacaaa atgtgaactt tatatttatc
     3361 gataaaaacg aacgtgtcat ttatccgact aatacagctt tactgaattt taccttaaca
     3421 gctgatcaat ggaaaaatct gaggaatgga cgtcaagaaa aattcacatc gaataaaaat
     3481 attttaggac aagcacaggc gacctcttac gcacttgtgc catttaattt gaatcatgaa
     3541 ttctatggag cacttgtagt tagtcaacct gcgcgaaata tcgataacag cgttcgtcca
     3601 attacactga acctgttcaa aggatttatt ttttccagta tcgttgcagt tattgccagc
     3661 tatgcttttg cagcattcca agttaagcgt atcaatcggt taaggaatgc gacgaaagaa
     3721 gtgacgaatg gtaattttga tgtacagctc cccgtacatg acaaagatga atttgatgag
     3781 ttagcagatg atttcaataa aatgacgaat tcattgaaag aatctcaagc agaaattgaa
     3841 gaacaagaaa atcgtcgaag acaatttatg gcagatgctt ctcacgagat gagaacacct
     3901 cttacgacaa tcaacggact tctggaaggg ttagaataca atgcaatccc tgaaaatcag
     3961 cgagaaaacg cgattaaatt gatgaaaaat gaaacagagc gtctgatccg tttggtgaat
     4021 gaaaacttgg attatgaaaa aatccgtacg aatcaaatca gtatggtcat taaaaaattc
     4081 aatggaacag aaacattgaa aaatattgtt gcacagttag aagctaaagc agaagcagca
     4141 ggagatacat tgacattgaa agccgttgat gatattgatg tttacgctga ttatgaccga
     4201 tttgtccaaa tcatggtcaa tattattcaa aacgcgatac agtttactga aaacggccaa
     4261 atcacgatta ctttagaaaa aggctatctt gaaacgatca tcacgattga agatacggga
     4321 attggaatgt ctgaacaaca aatgaaaagt atctgggatc gttattataa agtagatcct
     4381 tcaagaaaaa atacaaaata tggtgaatca ggattaggtt taccaatcgt gcagcaactt
     4441 gtacgcttgc ataaaggaaa attagaagta gaaagcgaac taggaaaagg aacaaaattc
     4501 gctgttcgtt tgcctgacgt agaaatcaaa aaagagtaaa aaacaagcgg tataaaagat
     4561 catgaatcga tcttctatac cgcttgtttt ttactataaa atgattgatt tagcccgcgg
     4621 gctctaaacg atcaatataa ggactttgag caatagtagc atcatgagta aagagtaaga
     4681 taccttgatt ttcttcatga gctaaataag aaagcaagtg gagtagctct tggatctcca
     4741 atacactaag ttcatcaaat gtatcttcaa taacaatatt gtttcgcttg gaaagcaagg
     4801 catgaagtaa ttgcagcttt actttttcaa aagacgtcaa ctcagctgcc gcacaattaa
     4861 gcaaggaagg agagatatca agtttttctt gataggccaa acgatcttgt ttgatttctt
     4921 tttccggtac atccaacagg acatggtctt taagattcag ataaggaaac atcggccaat
     4981 ttttttggat gatcgcaaaa ttatccagtt ttgcaacaag aacctctttt ttcaagtgct
     5041 ccaatatttt atgctgtttt tcgagaggac acaaaataac ggtcaagtgt tcagcatgaa
     5101 tcaaatattc aggtttcatt acgtccaggc ctcctacttt gcaaatgatg gataaaaaaa
     5161 gtgaaaggga atagactaag aatgagacag catccactga gcccaataag agtttgggca
     5221 aatcccttaa gtatatctgt gaaaaaaaga tcattttgga aatcgatggt aaaaaaagtc
     5281 tgttgatcca tcggaattgt aagtccgctt accgaagtca agatatcacg gatatctggc
     5341 agttcagtag tctttaagac aagaaaattg atcctttgaa gaagcgcttc ataaactgac
     5401 tggaagctta cgatccacaa taaaaccaag atacaacaaa gaaagagagg aaagaacagc
     5461 tgccagaaaa taaaaaggaa taatttggtt ttagaaagac cagtcaggtg ccaattgatc
     5521 aattcctgct tgttttgcct gactgaatag taaaagaaaa agataaaaag gaaggcaaat
     5581 agaaaaaata aactgaatat gccttttata taaaatcggc tagctaattc taaagccgga
     5641 agaagctctg tagatgcaga agcgccaata aatgaaagcc gatcttggac ttgattggct
     5701 aaagccgatt gcaaatggta aagagtcagg catgctatca ataaaaaagc agaaaagctt
     5761 gaaaataaac aatagagtat tgtctgcttt ttgtaataaa ttaatcgagc agtagcatag
     5821 cggattattt tcatactttt cacctctata aaaagtatag aaaatgaata tgaataaagt
     5881 atgaaattcc tttagtgaaa tcacgaatta aaaccagaaa atattttgta aaaagcaaga
     5941 ggttgtgaag aagctgccct attctattac cgtagatgca agcttttgaa cactgtttat
     6001 tcgaaaagag gagcagcgac aataatttt
//
LOCUS       DACCYH010000128         6013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.134, whole genome shotgun sequence.
ACCESSION   DACCYH010000128 DACCYH010000000
VERSION     DACCYH010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.134"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..450)
                     /locus_tag="IVY85_002718"
     CDS             complement(<1..450)
                     /locus_tag="IVY85_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8045677.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            674..1465
                     /locus_tag="IVY85_002719"
     CDS             674..1465
                     /locus_tag="IVY85_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8045678.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1431..2384
                     /locus_tag="IVY85_002720"
     CDS             1431..2384
                     /locus_tag="IVY85_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HAP8045679.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2782..3756)
                     /locus_tag="IVY85_002721"
     CDS             complement(2782..3756)
                     /locus_tag="IVY85_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8045680.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3871..4305
                     /locus_tag="IVY85_002722"
     CDS             3871..4305
                     /locus_tag="IVY85_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8045681.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4430..5416)
                     /locus_tag="IVY85_002723"
     CDS             complement(4430..5416)
                     /locus_tag="IVY85_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAP8045682.1"
                     /translation="MEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGAFFRVHHFAPQIG
                     SPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQGRVQLGVGRGSPE
                     QVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPMFPQGSGKLRIEP
                     YSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQQANQLRAYKEAW
                     KQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYHRNPTIFGKTFAG
                     EPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIAPELGWR"
     gene            5844..>6013
                     /locus_tag="IVY85_002724"
     CDS             5844..>6013
                     /locus_tag="IVY85_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HAP8045683.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRAIKIMAEDGI"
BASE COUNT         1894 a         1134 c         1038 g         1947 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaaa ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaaaataa
      601 aatcgtttat ctatataatt aagaagaaca aagtcgaaca aaaattgact tttatgttaa
      661 aggagtaatt catatgctga aaaaagaaag acaagaacaa atcattacat tattagagaa
      721 agatttttat ttaactaatt cagaaatcgc taacaaatta ggcgtatcag aaatgaccat
      781 acgccgagat gtcactgaat tagcaaaaaa aaataaagta aaaaagctat atggtggcgt
      841 tgaacgaaag gaaccttata atcgagaact atcgactcaa gaaaagattg atacgaatgt
      901 agccgaaaaa aaatatattg gtcaagtcat gaacagtatg attcccgata attctactat
      961 ttatgtcggt gctggaacaa cgatcctata tgcacttcct ttcattcaaa agaaaaatct
     1021 atttgtaatt acaaacagtt tgattgcttt caactattta aagaatcata caagttatcg
     1081 gattttactt actggtggcg aattttctcc tattacagaa gagttcattg gggaagttgc
     1141 agaaagatct tttgaaagtt tgaatgtcga tatctctttc gctgcaacaa acggcatatt
     1201 tgataacaat gtgacaaccg ctcaatttat tgagggaaat attcagaacg cagcgctcaa
     1261 tgcagcaaaa atcaaatgca ttgttgcaga ttattcaaaa ttaaataaat cagacgtcta
     1321 tacttttaga aaattaaccg attttgatta tctaataacc gataatcatt tatcagaaca
     1381 ggatttcaat cattattcaa aatacttaac aatattaaag gagcccaaaa atgatactga
     1441 caatcacgat gaacccatcc attgattcca tttattttac aaaacacttt gaattaggta
     1501 cgatgacacg ctttgattct ccggtacgct atgtaggcgg caagggaatc aattgcggac
     1561 gagcttctgc tctactaggt tcatcagtta ttttaacagg gtttttgggc ggttattttg
     1621 gcagtatgat tcaagatatg ctgaaaaatg aaaatttgtt tgatttgcat tttttaccta
     1681 ttgcagaaga atcaagaggt gccgtcacga tcatgcatga tgatgaaacg cagacagagc
     1741 tagtggaagc tggtcctgaa atagcagata ctactatcaa aaaattaatg aatgatatca
     1801 aactaatcag taaaaataat tacctttcag tcattacaat aaatggttct gttaacaatc
     1861 cagatccctt tttgtatgct aaattattac aatatatcaa agaacaaata gatccttcca
     1921 cctatatatt aatggatata tctggacaac atctccgtga agtcttagca agtaagtata
     1981 aaccgatgtt catcaaacct aatatccatg agttttcaga tatcatccat caaaaaatta
     2041 ctcaaaaaag cgacataaaa caccatctaa aaacaaatcc actatttaaa gatatcgatg
     2101 ttatcatgat ttcttgcggc tcgtatggtg cagtagtcaa agtaaaagaa gaattttgga
     2161 atgttgagat ccctaagatt cctatcatta atacaacagg ctctggggat gccactgttg
     2221 gaggttttgc tcattcatta gaacagaatt atcctttaaa agaatgtgta aggtacgcaa
     2281 tggcttgcgg catgtcgaat gcacagcaat cagaagtcgg gaaagttatt ccagctgatg
     2341 taaaaaattt agcaaaaagc attaaaatca cagaaatgaa ataaatagta caaagtaaat
     2401 aattgtttat atgaaatgag acacaaagag actgtgacaa aaaacgtgtg taccgacccc
     2461 caaaagttag attttttggt ctaacttttg ggagtcgata cattgtcaca gtcctttttt
     2521 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
     2581 gagaagctat tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacgacc
     2641 tcttggtata aacggtgttc aaaagcttgc atctacgcta atgagtccaa ccaagtcaaa
     2701 aatgaggagc ttttttggct gtccttatta cttgatgcag ggcagttttt tcataatctc
     2761 ttttagatta aatccttttg gttacacaag gttctcttca aagaatgttg ccattttttc
     2821 aaatggaatg atgtctgtct ggtcgtatag atctgtatgg gatgctcctg gaatgatcat
     2881 gagttccttg ttgtcacctt ttagttcttt gaaagcgtcc tccccaaagt aacgtgaatg
     2941 agctttttcc ccgtgaatga ctaagactgc atgacggatt tctggaatat aatgcaatag
     3001 ttttgcattc ataagtgaca accctgatgt cctattccaa ccatcattgg agtttaaaga
     3061 acggacatga tatccgcggt ctgttttata atacgcatga tagtctttga caaattgagg
     3121 agcatcttca ggcaacggat caaccacacc tccagcacgt gcgtagtccc cttgtttgaa
     3181 atcaatgatg cgttgtgcat ttaattcttc acgcatttca tatcgggcat tttcatccat
     3241 aacatcgaag tagccatttg ctgttactcg agtcatgtca tacattgtgg aaacaactgt
     3301 tgctttgacc cgcgtatcga tagctgctgc attcaatgcc atacctcccc atccacaaat
     3361 accaatgatt ccgattctct cagggtttac atttttttgt acagataaaa agtctactgc
     3421 cgcttggaaa tcttccgtat tgatatcagg cgaagcaaca tatcgaggta ctcctccgct
     3481 ttctcctgta aacgatggat caaacgcaat cgttaaaaag cctctttcag ccatcgtctg
     3541 cgcatataga ccagacgatt gttccttcac tgcaccaaat ggcccgctca cagcgattgc
     3601 cggcaatttc ttttcactta cttcttttgg ttcatataaa tcagcgacta atgtgatacc
     3661 aaaacgattc gggaagctca cctttcgatg attgacttga ttgctttttg gaaatacttt
     3721 gtcccattct tgtgtgatgt ttaattcttt attcatattc tagactctcc tttgatcaaa
     3781 attgacattt gtttttgaat gtattattat gataaaactt aaacttgact ttaggtcaag
     3841 gtaaaaattt tattttagga ggattttttt atgtattcca ttggacaagt gtctgaaatg
     3901 tttggattgc ctgtttctac tttacgttat tacgacaaag agggattatt tcctgatatc
     3961 cagcgtgttt ctggtattag aaaattttca gagaatgagt tagaggcttt aagagtcatt
     4021 gactgtttga aaaaatctgg tttagaaata aaagacatta agcaattcat ggaatggagc
     4081 aaagaaggca gtaagacatt tgaggttcgt aaagaactat ttgaaaaaca aaaagaagta
     4141 gtagaaaaag aaatagcaaa attagaaaga gtactcgcta tgctaaacta caaaagctgg
     4201 tactatgaag aagcaataaa agccggcaat gaagaagccg tattgacaat gattcctgat
     4261 gatttacccc aacatgtaaa agaagcttat gtacattctc attgaacaaa cggataactc
     4321 actctccttc tctatgaact tcatgaaaac atgaataaac acctctaact gattgttaga
     4381 agtgtttatt ttttcactca agtatcctgc acttacaaaa atatcctttt taccgccatc
     4441 ctaattctgg agcgatatct tttacgattg tttcaagcaa gtgcatgtta tattctaccc
     4501 ccaaagtatt aggaatcgtg acaagtactg tgtctgcttc tttgattgct tcatcttcag
     4561 ctagctcctt gattaattga tcaggttcac cagcaaaagt ttttccaaaa atcgttgggt
     4621 ttcggtgata agcaagatag ccgacagcat cttgttgcgg actaccgtct tgtccaaata
     4681 agcgtcgatc caaatcagtg gtgattggct gaatcgagcg agtaactaat gttcttggtt
     4741 caaaatcatg acctgcttgc ttccacgctt ctttatacgc acgtaattgg tttgcttgtt
     4801 ggatatggaa tggctcgtgg gattcgtaat tgactaatgt agatgattgc agattcattc
     4861 catgttgtgc tgcccagatt gccgtttgct gcgaaccagc tccccaccag attctttgtc
     4921 gtaatccttc tgaataaggt tctatccgta attttcctga tccttgtggg aacattggtt
     4981 gaggatttgg ttcttcaaat ggttttcctt caagtaattc caagaattca agggtgcgtt
     5041 cccgtgttac ctctttgatt tcttcttcac tgtaatcata accaaagtat ttccagccat
     5101 ctagaacttg ttcaggtgaa ccacgcccca ctcctagttg cactcttcct tgcgtgatga
     5161 tatctgtcaa tccggcattt tctgccatat aataagggtt ttcatatcgc atatcaataa
     5221 gtcctgtacc gatttcaatc ttacttgtct ttgcgccgat tgccgctagc aaaggaaacg
     5281 gcgacccaat ctgaggggca aaatgatgta cgcggaaaaa cgctccgtcc actccgattt
     5341 tttctgcttc caccgctaaa tcaatcgatt gcaaataagc atcttgagct gatttcacaa
     5401 gcgatccttg ctccatccaa tgtccaaagc ttaaaaagcc gatttttttc aaggtaatga
     5461 cctcctttaa acctacaaaa atggaaggtt ttttattttt aatatgaact gattctacta
     5521 ctttttttct taaaagacga ataattgttc tcgcctcaca atcaagaaat gtatcggctt
     5581 actacacccc tactttcttc atcatctgcc gtgtcttaat ctcttttaac agtctgttac
     5641 atcgttgatt tttgactcaa aatgattgtt tctaacatgt atttatggta aaaaagacaa
     5701 gattagttct ttgtacacat cgatttatac gctgaacaaa aagaaatata taaactaaga
     5761 aacccattgt tttttcgctc catcacatag agtttacttt agataaacag tgacatttac
     5821 taatccataa cggaggaaat caaatgaaaa aaatctattt cttatgtacg ggcaattcat
     5881 gccgcagtca aatagcagaa ggctacgcgc gtaaatactt gtctccttca atgtttgaga
     5941 taagaagtgc ggggatcgag acacatggct tgaatccaag agctataaaa atcatggcag
     6001 aagacggcat cga
//
LOCUS       DACCYH010000129         5942 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.135, whole genome shotgun sequence.
ACCESSION   DACCYH010000129 DACCYH010000000
VERSION     DACCYH010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.135"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(168..626)
                     /locus_tag="IVY85_002725"
     CDS             complement(168..626)
                     /locus_tag="IVY85_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8045684.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(639..1964)
                     /locus_tag="IVY85_002726"
     CDS             complement(639..1964)
                     /locus_tag="IVY85_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP8045685.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1981..3393)
                     /locus_tag="IVY85_002727"
     CDS             complement(1981..3393)
                     /locus_tag="IVY85_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8045686.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(3410..4174)
                     /locus_tag="IVY85_002728"
     CDS             complement(3410..4174)
                     /locus_tag="IVY85_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP8045687.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4278..5153)
                     /locus_tag="IVY85_002729"
     CDS             complement(4278..5153)
                     /locus_tag="IVY85_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8045688.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5408..5492)
                     /locus_tag="IVY85_002730"
     tRNA            complement(5408..5492)
                     /locus_tag="IVY85_002730"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5456..5458),aa:Leu,seq:caa)
     gene            complement(5510..5581)
                     /locus_tag="IVY85_002731"
     tRNA            complement(5510..5581)
                     /locus_tag="IVY85_002731"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5547..5549),aa:Gln,seq:ttg)
     gene            complement(5591..5663)
                     /locus_tag="IVY85_002732"
     tRNA            complement(5591..5663)
                     /locus_tag="IVY85_002732"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5628..5630),aa:His,seq:gtg)
     gene            complement(5725..5809)
                     /locus_tag="IVY85_002733"
     tRNA            complement(5725..5809)
                     /locus_tag="IVY85_002733"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5773..5775),aa:Leu,seq:caa)
     gene            complement(5830..5900)
                     /locus_tag="IVY85_002734"
     tRNA            complement(5830..5900)
                     /locus_tag="IVY85_002734"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5866..5868),aa:Cys,seq:gca)
BASE COUNT         1824 a         1260 c          903 g         1955 t
ORIGIN      
        1 ttttaagaat cctgaaaata agttttctaa atcataggag caacaaaaaa attcgataat
       61 taacaaaaat caaaatgatt aaatctagtg attgaaacat atcatttcaa aaaaattttg
      121 taaattcacc cataaatatc accctattta acttttttca tcgtacacta aaattcaaaa
      181 caactatctt taatctttac ttcttggttt tgttttaata aatttccttc aaaaatgcct
      241 ggagaaacta caatgatcac cgtatctttt tgtgcttgct ccaacttctt caaatctata
      301 atagctaaca gtgtcccttc ttttacctcc tggttacttt caacccgtat ctgaaacgga
      361 cttccttcga gttcaactgt atctaaaccc atatggatca gtatttttct gccagattca
      421 ctttcgatcg ttatggcatg tcgggtagga aatatattat cgattattcc tttaactggc
      481 gcatggatat atccatcatg attcgttata gcgtatccaa ctcctatcat ttttgaagaa
      541 aagacctcgt cttttactgc tgttaatgga ataatttgac cactagctac agcttttaga
      601 atgtttcttg ttttaaagaa atgcactttt tatcacctct actttaatgc tggccaatat
      661 tttttgttta ctttgatcat ttcatctagt attttttttg cgactctggc atttggtacg
      721 gtttgattca atgtaaatgc ttgtaaagct ttttgataag aaccttcaaa gtaagcatct
      781 actagtaact tctctactgc aacttgtgcc tccatcaatc ctttatgaaa atcactgatt
      841 ggttttctta gtgtaattgg ctccacacca gttgcaccaa catatgccgg tatttcaaca
      901 actgcatctg accgtagatt cggaatcgcc ccttgattag gaacgattag cataaagcga
      961 cgacgatcat cattcaacaa ggaaatagct atatcgacga tatattgtcc atgctcccca
     1021 aaattaaagt tcaatacttc ttccacaata ccgcggcgta ttttatcagc catttctttg
     1081 gtatttttct ctcgtccatc catgaccata ttagcacgtg tatactcttt gtcagtatgc
     1141 tcaacgacca tatcaggata taagtaatac tctaagtaat tattcgggat attacttgga
     1201 aaatttttcg tgatgactga catcatttga aaggtttttt gccaagtttt atctcctata
     1261 ttaaaatcgg cgacttgcat ttcttttgtt attagttttt caataatctc tggcataatt
     1321 tctcgttgca attctttatc ataaatcgat gtataccagc caaagtggtt taaaccataa
     1381 tacgtaacga tccaattctt acgatcataa ccgtaattca ctgcaatcgt ttcttcaatt
     1441 gaaatagtca tatcacaggc attaatcatt tttacattcg gatatttacg acgaacagct
     1501 tcagaaacga tcgactctgg atttgtataa ttcaaaatcc atgctttggg ggcatagtct
     1561 tgtatatgat cgacaatttc caataaacct ttgattgagc gcatcccata agcaaatcct
     1621 ccgagtccac acgtttcttg tcctactaat ccatgtttaa gtggaatctt ttcgtccttt
     1681 tctcgcatct ccaatcctcc aacacgaatt tgtgagaaaa caaaatcgca tcctgtaaaa
     1741 gcaagctttg gatcatccgt tgcacgaatg cgaatatttg aaaaaccttt tcttttcagc
     1801 ataaatttaa caatcaagta catatccata tttctttctt catcaatatc atataaacgt
     1861 atttcagata atggcaaccg tttttcattg ttcaaaatgg cttgaacgat tcctggtgta
     1921 tacgtactcc cgcctccagc tatagtgata atctgttttt tcatcatcat cttttccttt
     1981 ctattcttct aataatttat tgaattctgc cataacataa gtcacttcta agccgatgat
     2041 aatttgataa tttttttcag ttggtttaat gatcccgctt atccctaatt gcttgattcg
     2101 ctgttcctcg accatggtgg gatccactac ctctacccgt aggcgtgtat tacaactata
     2161 gacatccagg atattggctt ttcctccaaa cgcttgtagc atttttgttg ccatatattc
     2221 ataattttta cttgctaacc ctaactctgt ttcttctaag gttacttcat tgccaaactc
     2281 tgccttagct tcacgtccta atgtctgaat gtttctctta ttgatcaaat aataaaaagt
     2341 aacgtaatac aaggcaaaaa agaaaattcc gattggaaga atcaaaatag cattggtagc
     2401 gattcgaaaa ttgatgagat aatcaacaat acatgctccg aatgtaaaac caattcgtat
     2461 attgaataag taacagacaa tccctgctaa accggcaaaa agtgcatgga tcacgtacaa
     2521 cataggagaa agaaacatga aactaaattc aacaggctca gtgatatttg caataaatga
     2581 agttcctgca ccgcttccca tgattccttt gaccatctct ttattttctt tataagctgc
     2641 tttatagata gctagacata tagctggtat accaaacatc attactacaa aaaaaactga
     2701 taaaaacagt ccagcctgag gatctccgtt gataaagcgt gtcatctctc ctctgaaaac
     2761 ttccttgcta ggagatgtat agcttcctaa atcgtaataa atatacgtat tcaacacatg
     2821 gtgcagtcca aaggggatca gcaaacgatt caaaaacata aatatcccca cacctaatgc
     2881 tcccatatca acgagcagtt ttgcaaaatt atcaatccca atttggatga gcggccagat
     2941 aaagcccaaa aaaacagctg cgattgtttg tagaatcatc accatagtca atgggaattt
     3001 ttcgcccgca aaataagaaa aaactactgg taatttttgt tctttaaagc gattatacgt
     3061 ccatgccgct actaaaccgg aaataattcc accaaaaatc cccatattca cttctgggtc
     3121 cataatttct aagccttttt tcaaggtagc aatcgctaaa aaaccagtaa gtgcaggtat
     3181 tcctttgtcc ttatttttga caaaaccgat tgcaactcct atagcaaata agatatccaa
     3241 gtttccaaaa acggcatttc cagctgcacc aattactggg atgttcagca tatcgtcagc
     3301 tgataaacgc atgatcaatc ccgctaatgg catcgctgca actggaagta gcatcgctct
     3361 tccaagcttc tgcacatttg atttaacatt catttttatt cctcctctat taaatatcct
     3421 catatgttac taactcaatc aaaggatcat ttttcacttt ttgagcaaat gctgaatgaa
     3481 ttaaaaattc gacttggtgg gtacgaggat acacaaaact tgaaccattt aacaattcct
     3541 gatccaaata cgctgcgtga cacatgactt cgatcgtttt atcttctctc aatatcatga
     3601 gtaaacttgc caaatattct tccacactcg tattgatttg tcgttttttt tcatcagttt
     3661 ctcctacgac atcgaaacaa ggctcaaaat aatcgacatg acgaacttca tcttttcgtt
     3721 caaagttgct ccgaaccggc aaatcatatt tttttgctaa tgtaacaact acctcctgat
     3781 tttcatccaa agtatgagta tgatgatgac tgtctaaatg agttggtatg atccccgcat
     3841 gataaatttt ttgaatctga gcattccatt cctgatacag ttgatctttg tcaatgttaa
     3901 atggatgctg ataataagat aaggatctga actctccgct ttcgataaga gtatcgacag
     3961 ttttgaggag tggtttgcca cacgtcaatg ttaaatggac tcctacacct agcctaggca
     4021 tttcctttct tagtttcaca gcatgctcga aacccggcat atttgccatc aatgtagttg
     4081 atgtcaaaat tcctcgctga taagcatcca taatcccata attcacacca tggctatagc
     4141 caaaatcatc cgaattaaaa atcacttttc ccatttcatt ctccttcttt tttctactaa
     4201 aataggcgca accaaataat catagtccat ttactatgtg tcatttggtt gcgcctatag
     4261 atagttacgc ccttatttta atagattctt gctagatgaa ttgccaaata gatgatttca
     4321 ttttcttgaa tggcaagttg aaattgctgt tcgatgatat ctttaatctt tagtgctgta
     4381 caatacgctt tgctatgttt aaatgtttca gtaaaaaaac caatattttc tactggcgtt
     4441 tcttcctgat aaatcaagcg tttaaccagg aatttgatat gaaccattaa gcgatgataa
     4501 gtcagtgatt ctttatcata aacgtttgaa taggattttt ctagatggga caaaatcgtg
     4561 gtcgtcattt ctactatttt taagctcttt aaactattag gctctcctag ctcagcatta
     4621 atgatatgca tagtgatgaa tcctacttca tttgacccta actcggtttg atatcgatga
     4681 ttgattcgct cgaccacttc tactgctata tcatattcat ttggataaag gaatctcatt
     4741 tctggcaaca taattgctgt gacatctcta tcttctcttt ttcgttgaat agcaaaataa
     4801 atatgatctg tcaatgtaat atagatattt gaatctaatg gagaggagat cctagctgtt
     4861 atcagttcaa tcacttcttc cgcaaaatcc ataaattcga ttggaatctg cgagactatc
     4921 tcactgaatc gtttgatctt ttctttagaa tctaatacaa ataatttgtc agcatcctca
     4981 acatcaaatg gcttactcac tttagcagaa aagccaatcc ctttccccat cgcaattttt
     5041 tctattccat cgtcatcgat caatacagca ttatgattca gctttttcat cacatacacc
     5101 gttaattcac ctcctctata tgataaaaca aaacaacttg cagactcctt catacatatt
     5161 tttgtctcca aacaattttt aaagacaaaa aacgacctaa gaaagaaccc tttcttaggt
     5221 cgtgcctaat tgaatagtag caccctacat aatttacaaa aatagtttaa cacataataa
     5281 aagcgttatc aatatctttt ttactcgttt tatgataata cttgtatcga attgatttgg
     5341 aggtaattac ataaaaacaa aaaaaatata gcaactctct ataagaaagt tgctatattg
     5401 ataattatac cggcggccgg ggtcgaaccg gcacgtcctc aacggacact ggattttgag
     5461 tccagcgcgt ctgccaattc cgccacgccg gcaaatttaa aaataataac tggggtagct
     5521 ggattcgaac caacgcatga gggagtcaaa gtcccttgcc ttaccgcttg gctatacccc
     5581 aagaataaag ggcgaatgat gggaatcgaa cccacgaatg ccggagccac aatccggtgc
     5641 gttaaccact tcgccacatt cgccatcata aaagatgtta tttaatttct caataaaaaa
     5701 ctaagatgaa gacatcttag tcaataccgg cggccggggt cgaaccggca cgccctcaac
     5761 gggcactgga ttttgagtcc agcgcgtctg ccaattccgc cacgccggca agttgattta
     5821 ttagtactaa ggcggtaacc ggatttgaac cggtgatgaa ggttttgcag acctctgcct
     5881 taccacttgg ctataccgcc attatactat tatcaatcta taaaataaag aataaattat
     5941 ag
//
LOCUS       DACCYH010000130         5779 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.137, whole genome shotgun sequence.
ACCESSION   DACCYH010000130 DACCYH010000000
VERSION     DACCYH010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.137"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..813
                     /locus_tag="IVY85_002735"
     CDS             <1..813
                     /locus_tag="IVY85_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP8045689.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            807..1229
                     /locus_tag="IVY85_002736"
     CDS             807..1229
                     /locus_tag="IVY85_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045690.1"
                     /translation="MVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2363..3331
                     /locus_tag="IVY85_002737"
     CDS             2363..3331
                     /locus_tag="IVY85_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8045691.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            3341..3829
                     /locus_tag="IVY85_002738"
     CDS             3341..3829
                     /locus_tag="IVY85_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8045692.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            4125..4406
                     /locus_tag="IVY85_002739"
     CDS             4125..4406
                     /locus_tag="IVY85_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045693.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            4408..4824
                     /locus_tag="IVY85_002740"
     CDS             4408..4824
                     /locus_tag="IVY85_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045694.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
     gene            5438..>5779
                     /locus_tag="IVY85_002741"
     CDS             5438..>5779
                     /locus_tag="IVY85_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP8045695.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2154 a          982 c          947 g         1696 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcccttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tttctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgcgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaaatagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaact cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctcgaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaagc
     1441 cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca aacgatacgt
     1501 ccataggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat ttggctagca
     1561 atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt cgagtgagcg
     1621 aatccaaatt tgcaactctg aagctttgca aatgagtgtg ccggcttccg taaaagtcta
     1681 ggtaacatta gtcgatatat acatagggct ctaatttgcg ttttaaggcg ttttaaacgt
     1741 tctggtatgt aattatacca aagcgacttt aaaacgcctt aaaacgaatt ttagaggttc
     1801 tgtagaacgt tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg ctttaaaaac
     1861 aagataaggg attttatcta gacttgtctt aaaacgccgt agatgagcgc tcagagcttc
     1921 taaaaggact ccttacgaac tgtttttaag caaaggagcc tttaggcgac tgcgcagttt
     1981 aatgtgagag cgtagcgatc acacctttaa agcgaccgta gggagcagat tcccagttta
     2041 ggaaatttcg cgaacctgtt tggagaaaaa aagtaggaaa attttaggcg ggaaattttg
     2101 cccccctctt ttataatctc ttttataacc tctttttaaa cctctttacc gtcggctgta
     2161 tccttactct cccaaggtgt gcagaatctt ttaggggtct ttttgtctgt ttttaggggt
     2221 ctttttgtct gtttttaggg gtctttttgt ctgtttttag gggtcttttt gtctgttttt
     2281 aggggtcttt aattaggggg ttgaccccca ttagaaagct ctttataatt ttgttaagta
     2341 gttaatcttg aaggaggatt ttatggcaaa tgaaattgtg aaataccacc atgaattaaa
     2401 tacgattcct ttacgcaaat ttacgtcagt agaaatgaat ttattcttct ctataatatc
     2461 tagaatgcgt gatgtaggcg ataaaaaagt acaatttaca ttcgaacaat taaaagattt
     2521 aagcaattat aaagcaactg caaatgttcg attcatagat gatttagaaa caacgtatga
     2581 caaattaatg gatttacgtt ttggtagaag aagtgctgac ggtttgcaac gtgaacgatt
     2641 tgttttattt aatcaattta aaatcgatgg aaaagctgat attccttttg ctgaaataca
     2701 agtccatgag aaagccttac ccttacttaa taatcttgaa gaatgggttc gatattctct
     2761 ccaacaattc aatgaattag aaagcagtta ttccaaaacc atgtttagat tattaaaaca
     2821 atacagaacc aaaggaattg ccaatttttc aaaagaagac tttcatgaat tacttgatat
     2881 ccctaaaagt tatagagaaa gtgatataaa taaaaaaatt ataaagccta ttcgccaaga
     2941 actaaccgca atcttcaaag gtttgacaat ccaaaagaga tacggaaaag gacggggaaa
     3001 gcctgtcatt ggctatcaat ttacatttaa acctgagata aaaaaatcag acgactttaa
     3061 caaacatgct ccaaccaaag aagaacaaaa taaagctttg gaactagcta atcaacaggt
     3121 tcaatctgaa aaagaaccta cttcattaca agaaaaaatc aaagaacaaa aacgaaaatt
     3181 agaactttta caacagttag cagaattaga aagaaaacaa aataccccaa gtaagaaaat
     3241 taacaaacaa atagcagata acaattccga tattacccca gaatttaaag aaaacttact
     3301 agataatctc caaaacttat ttaaagaata aggggttttt atgacagata aaactatcaa
     3361 agaattagca gatattctag gtgtttcaaa agataaagtg aagtatcaac tgaggaaatt
     3421 acctagtaat tttacctaca aaaaaggaaa tattacctac ctgaaaaaag aagcagtaac
     3481 aacaattaca aaggttttat caggtaatga atcgggtaac ttacctagtg aattacccga
     3541 ttcattacct accgattacc tactcaagga gattgaaact aagaataatc atattgaaga
     3601 aaaagatagg caaattgaaa aacttcaaat agctttagaa acgcagcaaa aattactcga
     3661 ccagcaacaa caactcaccc tacaagccaa tcaacaaatc aaaaaacttc acgaacaact
     3721 ccagctaacc tatgcagaag aatcttcaga aaaagaaccc ctttctgtta ctgaagaaac
     3781 tcaaaaagga caaaacaagg agaaaaaatg gtggcaattc tggcaataac ccaacaattt
     3841 ttattcataa aaattgttgg gttactttct ttttttgctt ttacaagcat agcgacatga
     3901 tctcacgtcg ctatttttta aagagcataa ggaaacgcgg tatcactttc ttacgaaagg
     3961 tgctaggtag gctgtgccta ccacgtttct aagaaaaaaa tttgctatca ctttttcgtc
     4021 attgtggata gcttatgtat agtgtttcgg caaaaaacag ctatacaaat actatatttg
     4081 tatgatatac ataaattata cacaaatgcg aaaggaattc ttctatggac attttagttc
     4141 ggaatattga tccaaaagtt atttcagaaa tagatagacg ttgccaagaa ctcacaacaa
     4201 aaactggcaa aaaatggagc cgtaatgcgt acctaaaagt tctaatcgaa aacgattttg
     4261 accaggcact taggcattac aaaaaagatc aattcgattt attactcgaa aaatttatcg
     4321 atatacaagc aaaaaatact gaaacattga acgactatgt tcatacaaac aaccaactca
     4381 ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa acacgttacc
     4441 ctttatccca gtcacattga cgttttagat tccatcatcg aaaaaaatcc agatatcaat
     4501 aactactcgg aagccattcg ttttctatgt atgagttatg aacctaatac taatacgaca
     4561 actaccagca accaagcaaa actaaatgcg atgggaaaag aaatttctat tatgtccgaa
     4621 atcattgcag aatttgctga tttatatcta aaagatactg gtgttcttat cggtacaaat
     4681 tctaaagtat atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc ctcccgacga
     4741 aatcaaccca aaaatcgtca aaaagaaacc aatcaaacat tcacttctag aaattttgac
     4801 ccaacaaact ttctaaaaag atgaaaaaga aaccgaaaat tttttctatt tctcagtttc
     4861 ttttttaggg ggcagtcttc cagcaagatt tgcaagttac cggtaacttg cacaaaaaaa
     4921 gaaaaatccg cttgtgcgaa tccttctttt tgcattttgg ggcgctgccc caatctccaa
     4981 atatcggtta tttaatagcg tggtaactca aaaaaataga acgtactctg aaataaaaaa
     5041 agactgctct aacgagcagc caataaaaaa taatgagcgt ttattcttga aaaagcagcg
     5101 cgcttatgaa gcgctgccaa aaatttacca tattttatga aaaataacaa gtttctaaaa
     5161 aggaatcggt taagattcac aaagaatcgt aaacagacaa tagcggaaaa tcaagcttcc
     5221 gaacgtacct tttagctaga ccaatgaaaa atcacaaagt gagcggctgg atcggttggt
     5281 acaggggcta cttggcatag tcgacattat gttaactaac gctataaata acgattatgt
     5341 tcccaaaaca atattttatg aactataaca aaaatttgtt atcgcatatt gtatatttta
     5401 attataggtg tataccactt aaatttaaca caaaggcgtg attattttga aacccaaaaa
     5461 gaaaatttat ctaggtctcc tcttttcctt gatgattgga atttttagta taggaattac
     5521 aaatacctat gcttcagaaa attctaattt caatatcaac tcccaaccaa ttgaagaaca
     5581 agcaattaat tttgcgaaaa ctgattttga acactccata acttcaattc ttttaatgga
     5641 aaataacacc gaaactgata ttaactatac tcttggttcg ccatttaaaa tgctcgatgg
     5701 agaaaatacg gcaaatagtt ctattttctt tccagtattt tcagacgctg ataatacgat
     5761 taaatatatt tataccgtc
//
LOCUS       DACCYH010000131         5744 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.138, whole genome shotgun sequence.
ACCESSION   DACCYH010000131 DACCYH010000000
VERSION     DACCYH010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.138"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(191..712)
                     /locus_tag="IVY85_002742"
     CDS             complement(191..712)
                     /locus_tag="IVY85_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP8045696.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(715..1557)
                     /locus_tag="IVY85_002743"
     CDS             complement(715..1557)
                     /locus_tag="IVY85_002743"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP8045697.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(1550..2275)
                     /locus_tag="IVY85_002744"
     CDS             complement(1550..2275)
                     /locus_tag="IVY85_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP8045698.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            2451..2747
                     /locus_tag="IVY85_002745"
     CDS             2451..2747
                     /locus_tag="IVY85_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045699.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            3315..4907
                     /locus_tag="IVY85_002746"
     CDS             3315..4907
                     /locus_tag="IVY85_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8045700.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            4920..5660
                     /locus_tag="IVY85_002747"
     CDS             4920..5660
                     /locus_tag="IVY85_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8045701.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         1638 a         1127 c         1160 g         1819 t
ORIGIN      
        1 aaaagctgca cttccctttt ttatgtttct ccactaatcg aaatttagat agacatctag
       61 aaatcgcaat tccaaaaaat tctgtgtatt ggatacgcat cttttcttat tttttcttat
      121 ttcttccata tgggtgttta tctcagagat gatagaatag tttctaggat aaaaagtagc
      181 agctacgatg ttatccttcg tagaggattc ctgcttcagc tagtttttgt ttgatgtttt
      241 tcagcatatg cttttctggg tagcggatcg tatgcagatg gatgccgcct gttaattcgg
      301 ataacaaagt agcattactt ttttgataac gtctcataaa ggaatcgata tcttgtggcg
      361 ttttgatgtg caaagttcct gctaactcac cgtaaagggg atgttccaca attacatcaa
      421 tgatttcacc accattttca acgattagtc tcagttcttc ttctgtttga tcttttccat
      481 gctggacagc gattttactg atgtgcccag tattggtttc ttctgtttcg aggatataac
      541 ctcttgctgt tgccacgata cttttacctg cagctcgtag taaagcaata tcgcctacaa
      601 tgatctgacg gctgactcca aaagttttcg caaacttgct agcactcact ggtttttctg
      661 tatttgccaa ggtttgcatg atttgttttc gccgtgtctc tccttcgatc acacttattc
      721 ctccttcatt ttgttttggt attcgcgcga gtgtttcatc aaatcaacat acgtgtctgt
      781 tattgtttcc ttatatccct tatttttcag cagactaaca actttatcca atacttgctg
      841 ctctcttttt gcatcataaa ctgcttggtt ttcttctttt ttcacttgtc ctattttagt
      901 taccgtatcc attctttttt ctaataaagc aaccaactct cgatcgatca tatcgatctt
      961 atgacgtaat gtttcaagag actcttctgt tagttcttcc tctactgtct tgatgatacg
     1021 agcaggattt cctgcaatca ctacattgtc gggaaaagat ttggtcacaa ccgaacctgc
     1081 gccgactact acgttgtccc ctagagtgac acccggcgtg acgataactc ctccgcctaa
     1141 ccaaacatta ttaccgattt taatgggttt tgcgtactcc agaccgctgt tacgtttcac
     1201 tggatgtagt ggatgcgtcg ccgtgtaaag ctggacattc ggagcgaaca tgcagttatc
     1261 accgatttca atcgtgctga catctaaaaa tgtacaattg aaatttgcat aaaaattctc
     1321 cccaacaaat atattatatc cgtaatcaaa attgataact ggctccatat agattttttt
     1381 ccctgtacgg ccaaaagtct ctttcaacaa ttgggatcgc agttccgctg attctgcttg
     1441 gttgatgatt tggctttgac ttcttgcaaa catacggtcg gcaaccagct caggatcatt
     1501 ggcaaaataa agctcgcctg caatcatttt ttctttttcg gttttgaatt cagtcataat
     1561 ctgctcctaa ttcttgtatt tttttgaaaa caaccgctcc gtcttcttga ataaacgtat
     1621 aaggctccgc taaataaaca ggcgtttgat tgattactaa gattttggct gacggctggc
     1681 gaaactgtac cagatcacaa aacggatgaa cttgaaaact cgttccaaca atgacgatca
     1741 gctctgcttg gctgacggca taagccgcct gctccatgac tccatcttga aacccttcac
     1801 catataacac gatgtctggg cgtatttgtc ctccgcatgc ttgatgtttg tcgctgtgaa
     1861 gatattcttt ccagtctacc gtttgttgac attttcgaca atgacattga tacagactcc
     1921 catgaaaatt gacgagttgc gggcttttag cctttgcgtg caaaccgtcg atgttttggg
     1981 aaacaaccca aacatttttt tctttcgcta gatgtgctat ttccaaatga atgatattgg
     2041 gttttgcttc aggatgataa atctttttga caaattgata gaatttatcc ggttcattca
     2101 ccattgcttg atgactcagc aagtattccg gctgctccat cccgtggtag attcctgaca
     2161 aagatcgata gtctggcact ccagaaggtg tggatacccc tgcaccggtc aaaaatgtaa
     2221 tttgctgata agctaatatc tcttttgctg cttcgtctac tgttaattgt ttcatgtttt
     2281 gtcacctcac tttccattat agcccatact cgaacaaatt tattgttgaa aagtcagaaa
     2341 aatgggaata aaaaagtgtt atgggcattt gagcaattta tcacaatgtt cactgacaaa
     2401 agaatgaaaa cgtgttacaa tactgctgta taaaagtgag gtgaatcgat atgttaccat
     2461 tctttattta ttggggcgtt attctggctt gtatcgcctg gttagcgtta agcgtttatt
     2521 tctctgtttt ttatcttgta cgcaaagaaa atggaaatct ttgggctttt gctttattca
     2581 acgtgattgc cgcaattgtt ttagccatta cattagccgt atatcgaaca tggggctggg
     2641 gaatcaccca atactcaagt ttgatttatc taatattagc catttatggc gtcgttgttc
     2701 ttttgcaagc gatcctagga cgtgagccaa agaaagcggc agcttaaatg atcgagatgg
     2761 aactggacac aggaaatttt ctgttctagt tcttttttta ttgtattaca tacatttttg
     2821 tgtataatta tactatcgtt tgcaatggat agatgattca cattttgatt gttttttcgg
     2881 taaaagtaaa gatttctata tttatcagta ttttttcaaa aaccagcagt tatagattga
     2941 caaagcctct cgagtatcgt aaactttttt tgtataaata actgaatacg agatgcctca
     3001 atcaatgagg tagaggtcgc gagaatcatt aaccctgtgg agtgaagcaa acatggagaa
     3061 gcaggaatag ggaggatcgc cgaaatgtgc acttttttgc ttggagtgta tgttgggacg
     3121 caagttaaga gcttgcggac tgtcttagca gtgatgctaa gttgcgctat gttttgtgag
     3181 aggtttatca ccttatggaa ttttagcggc ctttttatat ctataaatcg gtcgcttttt
     3241 attttttcag ataaagcttt accgcatttc atctagaccg ctcgtgtatc atataatcag
     3301 aaagggagaa aaaaatgaaa aaaaatatgg tttcagttgc cgtactattt gcttttgtct
     3361 tctcactatt cagtgtggga tctctggcaa atgcagatga aaaaacaccg agcgatgagt
     3421 ttcgcgtagg tatggaggct gggtatgcac ccttcaactg gtctcaacaa acagatgaga
     3481 ataatgcctt gccaattcaa ggccaaaata gttacgccgg cggttatgat gttcaaatcg
     3541 ctaaaaaagt agcagatggt ttagggaaaa aattagtcat cgtccaaacg aaatgggatg
     3601 gtctggctcc tgcccttcaa tcaggcaaga tcgacgcgat catcgccgga atgagcccta
     3661 ctgctgaacg acgaaaagaa attgatttta ccgatcctta ttacgaatca caattagtag
     3721 tagtagttca aaaagatagc aaatatgcaa aagcaaaaag tttagcagat ctttctggag
     3781 caaagatcac tgctcagtta aatacattcc actatactgt gatcgatcag ataccagatg
     3841 tgcagaaaca acaagcaatg gataatttct cagcaatgag aacagcttta gcttctggaa
     3901 tgatcgatgg ttatgtcagc gaacgtccgg aaggtgtaac ggcaactagt gtgaacaaag
     3961 acttggaaat gcttgaattc agcggtgcag atggcttcca gacaaacccg gaagatgttc
     4021 agatttctgt tggtatgcgt aaaggcgacc cggatatcca aaaagtaaac cagattcttt
     4081 ctggcatctc taacgatgaa cgaaccaaaa ttatggacca agcagtcaaa gatcagcctg
     4141 ctgcaactga tagtgaagaa aaacctggca tcatttctga tttcactacg atttggaaac
     4201 aatacggcaa tatgttctta cgaggtgctg gacttacctt gttcatcgca ttgatcggga
     4261 cagttgtcgg tactacactt ggattactga ttggtgtctt ccgttcggta cctgaatcag
     4321 gaaatgcact gactcgcttc ttccaaaaaa tcggaaatgc acttctttct atctatgtcg
     4381 aagtattccg cggaacaccg atgatggtac aagcaatggt catcttctac ggtctggcac
     4441 tcgcttttgg catttcattg aaccgaacga ttgccgcttt gtttattgtc tctctcaata
     4501 ctggagctta catgacagag atcgttcgtg gggggatttt tgcagtagat gaagggcagt
     4561 tcgaagctgc gcaagccatt ggtatgaccc atggccaaac gatgagaaaa gttgttattc
     4621 cacaagtttt acggaatatc ttgccagcaa caggaaatga gtttgttatc aatatcaaag
     4681 atacagcagt tttaagtgta atcggcgtag ctgatttgtt cttccaagga aactctgctt
     4741 ccggtgcgaa tttccaattc ttccaaacat ttacgatcgt cgggatcatc tacttagtca
     4801 tgacctttac gatcaccaga atcctgcggg cggtagaaaa gaaaatcgat ggtccttcag
     4861 cttataccaa gcttgaagaa atcgacaatt caaatttgca ggaataagga ggaagaatca
     4921 tgaatgaaaa aattattgaa atcaaacatc taagaaaaag cttcggtgaa aatgaagtat
     4981 tgaaagatat cagcgtaaca gtcaataaag gtgaagttgt gacgatcatc ggttcttccg
     5041 gttctggtaa atctactttc ttgcgctgta tcaatttact ggaaaaacca acaggcggcg
     5101 agatcattta taatggagaa aatgtattag ctcctaaata taatttacct aaatatcgaa
     5161 cgaatctagg tatggttttc caatccttca acctgttcaa caatatgaat gtcttggaaa
     5221 actgtatgtc tggtcaaaca actgtcctga aacgagataa agaaactgcc aaaaaagtag
     5281 caatcgaaaa tctggaaaaa gttggtatgg atcgttatat cgaagccaaa ccagcacaat
     5341 tatctggtgg acagaaacag cgtgtcgcaa ttgctcgggc attatcaatg aatccagatg
     5401 tattgctatt cgacgaacct acttcggcgt tagaccctga aatggttggc gaagtgctta
     5461 atacgatcaa agacttagca catactggct tgacaatgct tatcgtgacg catgagatgg
     5521 aatttgcaaa agatgtatct gaccgtgtca tttttatgga taaaggtgtg attgcagaag
     5581 aaggtacacc agaagatatc tttgtccatc cgaaagaaga acgtacaaaa gaattccttt
     5641 cacgtatttt aaatgcttag gatcaatagc tgattgggac aaagagctgc ggtgtttacc
     5701 gcagttcttt tttatgaatt actcataaaa ggtaaatttc tgaa
//
LOCUS       DACCYH010000132         5634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.139, whole genome shotgun sequence.
ACCESSION   DACCYH010000132 DACCYH010000000
VERSION     DACCYH010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.139"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..253
                     /locus_tag="IVY85_002748"
     CDS             <1..253
                     /locus_tag="IVY85_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8045702.1"
                     /translation="TEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNG
                     VTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(396..1544)
                     /gene="nagA"
                     /locus_tag="IVY85_002749"
     CDS             complement(396..1544)
                     /gene="nagA"
                     /locus_tag="IVY85_002749"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP8045703.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1620..2456)
                     /locus_tag="IVY85_002750"
     CDS             complement(1620..2456)
                     /locus_tag="IVY85_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8045704.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2640..3857)
                     /locus_tag="IVY85_002751"
     CDS             complement(2640..3857)
                     /locus_tag="IVY85_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8045705.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            4240..4323
                     /locus_tag="IVY85_002752"
     tRNA            4240..4323
                     /locus_tag="IVY85_002752"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4274..4276,aa:Leu,seq:gag)
     gene            complement(4563..5560)
                     /locus_tag="IVY85_002753"
                     /pseudo
     CDS             complement(4563..5560)
                     /locus_tag="IVY85_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1754 a         1156 c          899 g         1825 t
ORIGIN      
        1 tacggaaggc tttcaagaaa cccttaaaac aatttcatct acgttacctc agtcgtttaa
       61 aaagaaattc acgatcttcc atcgctacca atcgggtatt tcaaatgcct ttaaggtgcc
      121 ccactcaaac ggcgtcactg aggggctgaa taacaagatc aagctgatta aacgaattgc
      181 gtttggttat cgcaactttt ataactttcg ggcacggatc tatttacaac aaggtctgat
      241 ctttgaaaac taaaaaaata tgcggcgcac agaacgcgcc gcatatcgga ctaagtttta
      301 ttcaaatttg tcttcgccaa cacaatttga cgaagagcca aaaaacctta aaaccaaact
      361 taagaagttt agttttaagg ttaacttaaa ttatattatg cgtggaaacg ttccacgcca
      421 tctagataag ttgcttccaa tgtcatatca gggttcaata cgataaagtc tgcatcgcgt
      481 ccttgagcga tttttccaca tgtatcatcg attttacagc tgattgctgg aatcagacta
      541 gccatcatga ctgcttgttc tggtgttgct aatccccagt ctacaacatt tttaattgct
      601 tctttcaatt tcaatatact tccagctaag ttgccagatt ctagacgtgc tgttccttct
      661 tttactacta ctggaaattc gcctaaattg taatttccat caggcatccc accggccatc
      721 atacagtctg taattaatgc gacatgatca tgtcctgctt tttccattag gattcccgca
      781 gcatttggat gtacatgatg gccatcacaa atcaattcag aaaagacatg ttctaaagat
      841 aataacgcac ccaccatacc tggttcgcga tgattcaatc cacgcatacc attgaaagca
      901 tggacaaata cacttgcgcc tgcttcaaca gctttttgcg cttcttctag tgtagcatta
      961 ctgtgtccta gagaaacaac tactccttct tcagtcactt gtttgacaaa ctcttctaca
     1021 ccttgacgtt ccggtgctaa agcaattttt ttgattaaac cacctgaagc ttcttgccat
     1081 tcatggaaag tatccaaatc aggatccccg aaataacttg ggttttgtgc ccctttgtgt
     1141 tcttctgtga agaaaggacc ttcaaaatag attcctttga ttttagctcc tggtgcttcc
     1201 tgatacattt tgccgattgt ctcagctaca tcttttaaac gttctttgct tgacgtcaat
     1261 gttgttggta aaaatgaagt cactccacaa gataacagac cttcagacat cactttgatg
     1321 ccttctgcat cattatccat tacatcatga ttcatatagc cgtgaatatg tgtatctacc
     1381 aaaccaggag caatccattt gccgctttgg tcaatgatgt tgacttctcc ctcaggtagt
     1441 tctttggtgt attcaccaaa aagtccatcg gtaatttcaa gatagcctgc atgtttgaca
     1501 cctgacgcta aaaagaattt gtcagcatag ataaaagttc tcattctatt ctctcccttt
     1561 gtttttctac ctctaaagta ctcttattta gagaagaagt caagaatggt ctacaccatt
     1621 tacaaatttt ttgaaatttg tttcacagct tgtgctaagc cttcatagaa tgctgctctt
     1681 tctggttctt ctttacgctt gtatattaag cttttaagca caaataaatc attgaggtaa
     1741 tagtagctat tcttctcttg agcccaatag attccttgat tcacctgttc taacgcttcg
     1801 tcgatttcat tttgatggaa taagaaattt ccataattat aaaaaatttg cgaaaattca
     1861 ctttttgatc gttcatcagc tgattgatgc agcagttgga tgcttcgact caaatagtag
     1921 cgtgcttcag ccgtttttcc tgccacacca tatatttttc cgatacaact aatcagttgt
     1981 atttctgtgt cagaaacatg gattctttct ttttggctag agtagcttaa tgcttctttc
     2041 aaataataaa gggactgaga gatattttga cgcaacgtga attcgcaaac accgagataa
     2101 taataatagc gttgaaagtc agttgcttga tacagatttt cagaaatatc agaagatttt
     2161 agtacttgtt ctaacttccg ataatcacgt ttttggaaac aactatccaa cagttcaaat
     2221 aatttatcgt ttgtccgagt agtttttcga gagagattca tcacatgatc aatcgttacc
     2281 cctagccgtt gacaaatctg catcatcacc aatacattcg gcagttcctc attattttcg
     2341 atccgactta atacactttg tgaacaaata tcatgcgaaa gcattttttg cgtcattttt
     2401 tgttcttttc gaatctcttt aatgatcgtt ccaaaggact cgattaacgt ttccataagt
     2461 atcctcccca aaatatgcgt attcgcattc tattcgtatt ttactttagg aagtcgcaaa
     2521 cttcaagaaa agacttgcaa ctaaataata tactttagtt atttaagaaa actccactag
     2581 aaagagagcc tcagtgtgtt tgtctgtggc cctcctactg aaaaatttgg tttttaaaat
     2641 catttttggc gataggttgc taggaattct ctcattttct tagctgtttg tttcaaatca
     2701 tccattttag gaagataaac gattcgaaaa tgatctggct ggttccaatt aaatccacca
     2761 ccatgtacaa gtagaatatg atgttcatgt aaaaagtcca aaacgaattt ttcatcatcc
     2821 aaaatattga atcgctttgt atcaattttt gggaatatat aaaaagcagc ttttggtttt
     2881 acggctgaaa gaccaggaat atcattgatt gcattataga tatattctct ttgttcatag
     2941 atccttccgc ctggcaggag cagctcatcg acactttgat aaccgcctag cgcggtttgg
     3001 ataatttgct gagacaatac attggaacac aaacgcatgg aggacaacat attcagccct
     3061 tctatatagc ctttgacgtg tgacttatct ccacttagga ccatccagcc tactcgaaat
     3121 cctgctacac gatgagattt ggatagaccg ttcagcgtaa caacgaacaa atcaggcgct
     3181 aatgtagcaa tcggaatatg ttctagacca tccatcacta agcgatcata gatttcgtcg
     3241 gaataaataa tcaggttatt ttgtcgagct acttcaacga tttcttctaa taattctttt
     3301 ggataaagtg ccccagtagg attattagga ttaatgataa caatagcttt tgtccgactc
     3361 gttatttttg atttgatatc atcaatatca ggataccatt ctgcctcttc atcgcaaata
     3421 taatgaacag gtgtgcctcc agctaatgca atagaagccg tccacaacgg atagtccggc
     3481 attggaacca gtacttcatc tccattgtcc agcaacccct gcatacacat cgtgatcaat
     3541 tcgctcactc cgttgccggt gtagatatca ttgatcgtca cattcggaaa atgtttcagt
     3601 tggcaatatt gctcaattgc ttttctagca gaaaagatcc ccttggaatc tgagtagcct
     3661 tctgagtcac gggcattcat gatcaaatca cgaatgactt cgtttggtgc ttcaaatcca
     3721 aaaggtgcgg gatttcccgt gttcaatttt aaaatactaa tgccctcttc ttgcatccgc
     3781 tccgcttctt ctagaacggg accgcgaaca tcgtaactga caccttctaa tttgttggat
     3841 ttttcaaatt ctctcatcac ttagccaatc cctcatttca aaaagttaga actttccaaa
     3901 ttttttgtta tttactattt caatatcata ctccatccta gaaaattttg ccacttattt
     3961 aggaaaaacg taagtattaa aaaaacacaa acaagcgttg agaacttaat tgtcatgata
     4021 gtcattccat agattcgctg cccaatccat tgcaattcca caaatgattc ctagcataaa
     4081 accaagcaaa aaacgtccga atgcaagtcc aatcaataca cctaagatca ttcccagtcc
     4141 tgaaaattct ccgtcatatt tctccatctt aataagtcct cccatttttg agcttgacaa
     4201 ctatgtaaaa cctcgctatc attagtgtgt tattattacg cagatgtgtt ggaattggca
     4261 gacaagcatg attgagggtc atgtgagcgt tagctcgtgt gggttcgagt cccatcatct
     4321 gcactacaac tcgtgaagtt aattttgctt cacgagtttt tttgtttgaa cgtttcttta
     4381 tgccattata tacatatcac tgtatccttt acaaaaagaa cgtacaatca tgtaaacagc
     4441 ctgaggtgag tatatgaagg tcggaaacaa gctactcgta gaaacttgca agtgttaaaa
     4501 caattaatcg aaaaacgagt aaaatccctc acctaatact aaagcgaggg attttttgta
     4561 tattatctgt ttatcaccgt tattataaag gtacaaatac caagcacggt taatccaaat
     4621 tgcataaatt catttttttg tacactcatg cttttcatta gctctttaac tgccattagt
     4681 actaagatgg cgataacaac ataaagtata tagtacgtcc gcattccgga agcccatact
     4741 gtgggagaaa atcccataat taaccttgaa caaaatccca ttatcatcaa aataactaaa
     4801 aaaatagaag ttttcttatt gtcaaaactt aaataaattc caacaattaa acatagtaat
     4861 aatgccaaga ctactaaagt agctatccaa gtacctggat aaaaaagtga cagtttcgtt
     4921 cctaactctg taaagagatt attcaggtta ctactactcc aaatcattgc ccttttattt
     4981 ccattgacgt aagtaaagct ttgacccatt gtattaccaa aataaataat caggtttaag
     5041 aaaaacggta atgcagtcaa tatacgcaca taatagtttt gacatttcct atatgttaga
     5101 aggaaaatta agaaaaatag caataaaaat aagatattca tgtctaaaaa aacggctttc
     5161 caaatgatga aatacctaaa tctaacttat taagaaagtt gaaattttta tattcaggaa
     5221 accatttatt aatttcatgt gcagcacgta atgcattccc tggagttgtc aaagagaaaa
     5281 ttaattctgc taagcttatt attgaaaata ctgataactt ataattatat cgtccattga
     5341 caatcaaata taaactaact atacttgtta gaacaaaaaa acaggcgttg acttgttctt
     5401 gattagttaa aaatatcaat aaaggaatac ttacccaata tatttttcta tttatttctt
     5461 ctcctcttaa gagttgaaaa aacggataaa aagttagtaa actaaatgct actggccact
     5521 gatagttcaa ggtggttgct ctccaaccgg tttcccccat tactgtcaat ggtattaaac
     5581 agtatataga gcaaaccaat aataaatttt taacatttat tttctcaaaa acat
//
LOCUS       DACCYH010000133         5333 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.140, whole genome shotgun sequence.
ACCESSION   DACCYH010000133 DACCYH010000000
VERSION     DACCYH010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.140"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            32..388
                     /locus_tag="IVY85_002754"
     CDS             32..388
                     /locus_tag="IVY85_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045706.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            404..754
                     /locus_tag="IVY85_002755"
     CDS             404..754
                     /locus_tag="IVY85_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HAP8045707.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            783..1562
                     /locus_tag="IVY85_002756"
     CDS             783..1562
                     /locus_tag="IVY85_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HAP8045708.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1573..2235
                     /locus_tag="IVY85_002757"
     CDS             1573..2235
                     /locus_tag="IVY85_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HAP8045709.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAIFPA
                     VN"
     gene            2254..3360
                     /locus_tag="IVY85_002758"
     CDS             2254..3360
                     /locus_tag="IVY85_002758"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HAP8045710.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3350..4441
                     /locus_tag="IVY85_002759"
     CDS             3350..4441
                     /locus_tag="IVY85_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HAP8045711.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4454..5194
                     /locus_tag="IVY85_002760"
     CDS             4454..5194
                     /locus_tag="IVY85_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HAP8045712.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
BASE COUNT         1718 a          870 c         1267 g         1478 t
ORIGIN      
        1 acaaatgata ttttacgagg aggaaacaat catgattaaa gtagttattg cagaccgtat
       61 gggtaaagga caaaatgtgg ccaaaggggt agaagcagct ggtggaaaag cagttgtcgt
      121 accaggaatg ggggcagata tgcgtttggg cgatgtgatg caacaagagc atgcagatat
      181 gggtatctct ttttgtggta gtggtggtgc aggtgcatta acagcagcaa caaaatatgg
      241 ctatccagaa cgacatggta tgcgttcgat tgaagaaggg atcacagcta tcaaggatgg
      301 caaaactgtt ctaggtttcg gctttatgga tcaagaggaa ttagggaaac gtctgacaga
      361 agaatttatc aaaaagcatg gaaaatagaa agagggacat gaaatgagcg ttcaaaagac
      421 aattcagcaa aaagttatcg tctccggtaa aggggaaaca aagcaacatg cttttgcatc
      481 agcgttagca gaaattcaaa acaaaataat ggaacaatca aacgtattat tgcgaatcga
      541 accagtcaat gtggatattt taaaggcggt cgaaaacaaa tacacagaac gctttctatt
      601 tttcttcttc ccacggcaac gtattagcta tgaagtcgaa ctctctgtta ctgtggctgt
      661 aacagaaata aaattggcag acatatcgtt cagccagcaa atgactgaag atccaaatgg
      721 gattgccatt ccatttgtaa ctaaaaaaat ctaattttga atcataaggg ggaaagaata
      781 acgtggattt cgtaatcatt ctagtcaaat cggtcattat cgggggatta cttggttttg
      841 cagcatctgt cggggcagct cgaatgtttc atgcacctaa tacacaaggt ttaggagctt
      901 ttagaacatt aggcgaaatg aatgcctgca tgggtgatcc agcttctcat ttttcatttg
      961 gtttaggctt tttctttaat gcatgggctt cttcagttgg tgctggtgca ttcacacaag
     1021 atgtgacgca tcgaatcatt cccaattggg cagcggcagc acttctagca aaaaacaaag
     1081 atgtcaccca gaccatgcat gatccgaaaa aaatggggat cattggagcg gttatcgggg
     1141 cagtggtggt agccttctta aacacaacag ctgcagcaat tccagaggcg ttacaagtga
     1201 cggcggtttc cgttttggta ccagcagcga cgattctgat caatacagta atgccagtta
     1261 ttttttggtt ggcagcttta gacgcaggga aacgaactgg tttttggggc actctttttg
     1321 gtggtatcgc gcaattgatt atgggaaatg cggtacctgg tgttgttcta ggaatcttga
     1381 ttggtaaagg cgtcgatgag agcggttgga atcgcgtcac gaagagcatg atgatcgcta
     1441 ttgttttatt atttattttc agtggatttt tccgtggatt tgatatggaa atgatcaaga
     1501 gcttccagct tcaagtgcct aattggcttg ataacttgca caacatcttt agtgtgaaat
     1561 aggaggagaa tcatggagac aacagaagaa caagcaaaag tattagaagc aaagcggcaa
     1621 aagagctttt ggtttgcaga ctggtctttt ccgctaatcg taggaattat gtccgctgct
     1681 gtatttgcag gaacacacat gtatgtgatg tatggtgtgg gtgcattcaa tgaagtttcg
     1741 atcgtggcga tgttaaaagc cgggctggat ggcggatcct atggcgcagc ggcagcgttt
     1801 ggtgctagtt tcttatttgc cagaatttta gaaggctctt tagttgggat cttggactta
     1861 ggcggttcga tcctaactgg gatcggaatt ggggtaccag caattctctt aagtatgaaa
     1921 atagcggcac caatcgataa ttttgcactc tctttattga ctggtttggt tctaggtttg
     1981 attgttggtg gcatcattgt cttgattcgt aaatttacga tcaatcaatc aaattccact
     2041 tttggtgctg acgtcatgat gggggcagga aattcatctg gacgtttttt gggtccatta
     2101 attatattaa gcgcgtgtgc agcatctatt ccaatcggca ttggcgctac attaggagca
     2161 ctaggattct atgcttggaa aaaaccaatt gctggaggtg ctattttagg agcaatcttc
     2221 ccggcagtaa attaatagaa agagggtcat taaatgtttg atactttgat taaaaatgga
     2281 aggttagttg atggatctaa aatagaggtc gctatagaaa aaggcatgat taaagcagtt
     2341 gcagcagaaa taaatcagcc ggcgcaagaa gtagttgact tggagggaaa gtattattta
     2401 tccgctgggt ggatcgatga tcatgtccac tgctatgaaa agatgaatct ttattatgac
     2461 tatcctgatc agataggggt cgaaaaaggt gtgacgacgg tcattgatgc tggaactacg
     2521 ggagcagaaa acatccgtga attttatcaa ttgacgaaat cggtcaagac aaatgtgtat
     2581 gcgctaatga atatctctaa atggggcatt gtcaaacaag atgaacttgc tgatctaaca
     2641 aagattcaag aagatctagt aagtactgcg ttagcagaac tgccggaatt tattgtcggg
     2701 attaaagcga gaatgagtaa gacagttgtt ggtgaaaatg gcattaagcc attagaatta
     2761 gctaaaaaaa tccagacaga aaacaatgat ctgccattga tggttcatat cggttctgca
     2821 cctcctgagt tagaagaagt attgtcaaga atggaaagag gcgatgtatt gacccattgt
     2881 tttaatggaa aaccaaacgg tattttagat ccaaagacag atcaaattaa aaactttgtc
     2941 tgggatgctt acaataatgg tgtcgtcttt gatattggac atgggacgga tagctttaat
     3001 ttccatgtgg cagaaaccgc actaaaagca ggaatgaaag ctacctctat tagtacggat
     3061 atctacatcc gcaatcgtac aaatggccca gtttttgatc tagcaactac tttagaaaaa
     3121 ttgagggtag tcggctattc ttgggaagaa atcattgaaa aagttacagc agttcctgca
     3181 cggaattttc atttgaagaa taagggccag ttagctgtca attatgatgc agatttgacg
     3241 atatttgaga ttgttgaaaa tgaaaaggaa ttgaaagatt ccaacggatt tacacgtata
     3301 gctaaagaac aaattttacc gattaaaaca attattggag gcgtcatata tgacaattag
     3361 ctatggaaag tttgatttaa aagaagtcat taatgcatcg ggaaaaatga cgatcctagg
     3421 ggtttctaag gtatctgaaa gtgtcctagc agcacaacga tttggaggcg agcatttttt
     3481 tgagatgagt gatcttgcca tcaaaaccgg cgcttatctt tctcaattac tgaaagtgga
     3541 agatgcgcaa atcgtttctt ctgcttcagc agggattgct caatctgttg ctgctttaat
     3601 cggacagggc gacatgtacc atgtctatca tccctataca gaaaaaatcg ctagacggga
     3661 aatcatcata cctaagggac ataatgtaga ttatgggaca ccggttgaag taatggttgc
     3721 tcaaggtgga ggaaaagtca tagaagctgg ctatgcaaat atgtgtacac cggaacatgt
     3781 ttccatgatg attacggaaa aaactgctgc gcttctatat attaaaagtc atcatgcagt
     3841 tcagaaaagt atgctgacgg tggcagaaat ggtagaagtt gccaaagctc atcgtttgcc
     3901 attgatcgtt gatgcagctg cggaagaaga tttattcaaa tatagcgaaa tgggcgttga
     3961 cttagtgatt tactctgggg caaaagcaat cgaaggtccg agtgccggac tggtcatagg
     4021 aaaaaagaag tatattcaat ggattcggct acagtctaaa ggtattggcc gagcaatgaa
     4081 aatcggtaaa gataatattt taggctttac ccaggcacta gaagattatt taaaaaatgg
     4141 gagcgaatcc ggtgactcca tgaagcaacg attagcgcct tttgttaacg caatcaatca
     4201 acttcctgac ctttcagcga aaatagttca agatggtgcc ggcagagata tttatcgagc
     4261 aagtgtaacg attaatggcg ataaaagtgc gaaagaagtc attcaggaat taaaagcacg
     4321 aaatccagca gtctacaccc gagaatatca agcaaataac ggaatcattg agttcgacat
     4381 tcgttccgtc agccaaaacg aaatggaaaa aattataaaa agattgagag aaatcatgta
     4441 ggaggctttt aaattgtcag ttatcccaaa ttattataaa aatcgtgtat gtttaaatgt
     4501 attagctcat tcagttgaaa atgcaaaagc atgctatgaa gcagcggaag ggcatgtcgt
     4561 actaggtgtg ctatcgaaaa attatgctag cgatgaagca gcaatcgaag atatgaaaag
     4621 gtaccaagac gcaaccaata atgcgttgtc agttggatta ggcgctggag atccaaacca
     4681 aagtcagatg gtttcgcgtt tatctggggt tttacaacca caacatgtca atcaagtatt
     4741 tactggcgtg gggacttctc gtgctttatt gggccaaaat gatacagtca tcaatggatt
     4801 ggtttcacca acaggtaaga tcggctatgt caatattgct acaggtccgt taagttcaca
     4861 agcgccagca gctgaagtgc ctattgaaac tgccattcaa atgctgcaag acatgggagg
     4921 tacttcaatc aagtatttcc cgatgaaagg cttagcacat agggaagaat ttaaagcagt
     4981 tgcggaagct tgcgcaaaat atgaattcta tttagagcca acaggaggaa ttgatctttc
     5041 aaactttgaa gaaattctga aaattgcaat cgatgcaggt gtaaaaaaag tgattcctca
     5101 tgtttatagt tcaattatag ataaagaaac aggagatacg cgtataggag acgtaaagat
     5161 actattaagc atggtaaaaa aaattattca ctaactcatt agaaaataag aagcttattc
     5221 cagaatgaaa ttaaatggaa taagcttttt ataattaatt acggaaattg ttaatgtaga
     5281 taataattta gatgtacgta agcagaagta cagaaatgta agattttcca ata
//
LOCUS       DACCYH010000134         5239 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.141, whole genome shotgun sequence.
ACCESSION   DACCYH010000134 DACCYH010000000
VERSION     DACCYH010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.141"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(662..952)
                     /locus_tag="IVY85_002761"
     CDS             complement(662..952)
                     /locus_tag="IVY85_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045713.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1294..1590
                     /locus_tag="IVY85_002762"
     CDS             1294..1590
                     /locus_tag="IVY85_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045714.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1597..3549
                     /locus_tag="IVY85_002763"
     CDS             1597..3549
                     /locus_tag="IVY85_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP8045715.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3621..4118)
                     /gene="dfrG"
                     /locus_tag="IVY85_002764"
     CDS             complement(3621..4118)
                     /gene="dfrG"
                     /locus_tag="IVY85_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP8045716.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4445..5137)
                     /locus_tag="IVY85_002765"
     CDS             complement(4445..5137)
                     /locus_tag="IVY85_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8045717.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1700 a          907 c          865 g         1767 t
ORIGIN      
        1 tacttaaaga taacttaaaa gttatctttt aaatacttaa ttgttaaata ccttatttga
       61 tgcaaaagaa atctacgata gtcaacaaaa aaattttcat tatatccaga aaaatttttc
      121 ttagttaagg acctatggat tgcagtaatg cagaattcgt cgaataaact gccaaagaca
      181 tacaatcata ttgcttttaa aattgacgaa caagatatag attcgtttgt ctcaaaaatc
      241 caaatgttag gtttaactgt cgaaccagga cgttctcgtg tgaaaggcga ggcttcttca
      301 atttactttt acgactatga taatcacctc ttcgagttac ataccggtac tctccaggaa
      361 cgtttaaaat cctacaattc cactacctga gactagtaat gactagtctc tttttatggc
      421 ctctgatccc cccattgagc gaatactaat agggcttcga attgatctgt tcaatcttta
      481 agcaatatcc ctcaaatgag tttggaaagt ttagtgattc atcacaaata cttaaataca
      541 tgtagttata tatagattga ggttcttgtg agcgtatgct cgtgtgggtt caaatcccat
      601 cgtctgcatc catctttcca aaacaagcgg ctaggacatt tttgtctcag ccgcttgttt
      661 tctagtttat tctttcatct tgtcggacct tttttcttgg gtatagactt ttctcatatt
      721 ctaccacaat tttgggtttc cctaacacat gataggatct cacaacaaat tgctttctat
      781 tattataata acctgtaacg actaacttca tcccttcgtc tacttcagct aggaagttca
      841 aactatggga agcaatcaaa caattgaccc catttaaact gaaacggatc aacggtctgg
      901 cactaacttg caaagtacgg atcttatcta ctgttccata ttttttctcc ataaccttca
      961 cctcaatacc attatacgaa catacattcc ctttgtaaag aagaaactct gttttatctt
     1021 tttgactttt tgtctattac ctttcttaaa atcatattgg cacttcctgc gaagatgaag
     1081 gacatgtact aaaaaaaaga ggcagcagta tagatttaag tagttcagtt ttggagtaca
     1141 aaaagtgaaa atgtaaacct aaatgagtat caaattgcaa ctgaatttga tactcgtttt
     1201 ttattgttaa atcaacgttt gttgttaatt tacaccacaa aatgtaagtt aaaatgtata
     1261 taaaaatgcg aacgtgaaat aattattgta ggaatgcaaa ttggtgtgtt aaaatggtta
     1321 caatttacta agcaaaaagg ggtgcatttt cctatgcaat ttcttgagcc aaaaaataag
     1381 aacgctaagt ctgtggattg ggaaatatca gagcaagtaa gggtgatagt taagcaatac
     1441 gctgaatatg cagagcgtac tgaaagcgaa gcagtggatg aattcttatt gaacatttta
     1501 gacgataaga agtttattga atggattgcc aacaagcgaa gtaataagcg gattgttgaa
     1561 aaaatgggta tcaaagatag agtgggttga tagacaatga ccaagcttaa aagattagcc
     1621 acgaaggaag atgaaattgt agatgttaaa attccgattt caaacgaaga attaaaagat
     1681 agggctaaac aatatgattt attaactcca aaacgatttg caacaaggta taacaaaatg
     1741 ttgttcttgc caacgtcttt taaatggaac ggttctgaat atcccattca atataattac
     1801 tgcatcaatc cgttttgttg taattttggg aaagaacagc acaaattcaa agatgtaaaa
     1861 gggaaaccaa gtcgatataa aatgactggt agttccaaag ataaaggtca taaaggtatg
     1921 tactgcaatg acaatcctat tggcagaggt gtatcgcaaa actgtacagt aaccccttta
     1981 tctaattggt ctgttgttga agaaattaaa cgccttatag aaatcaatag catccaagat
     2041 gttgagccag attatcaatt tcataaagaa ggctgctcgg aagaagaatc aaccccattc
     2101 aatgagccga agcaatttta caaaaggggg aaaagtaggg gcaaatctca acgctatcaa
     2161 tgtaaggcgt gtaagaagtt cactaatgtt ctaccaaaaa gagaagaaac cactacttat
     2221 caccaacaaa agaacaccat tcttccaatg ttcgctaaga tggttgttgg aagggtttca
     2281 gtcagtcgta cttgtgatat tttaggaatt ggagttggaa cctactatca taagctggag
     2341 tggctgtata ggcgttgttt ggagttttta gaacgatatg agacacaacc ccttcaaaca
     2401 aagaaattta acgagatgtg gcttaatacg gacaagatgc actactacct aaataacgtc
     2461 cgtaagaaag gacaaggttc aaagaaatat acggggtttg aagaccttaa tatgcaaacg
     2521 tatatagttg tatctgctga ggtcttatca agatatgtgt tccgttctga tgttgcctat
     2581 gattggaata tttcaatgga tgaattaaat gaagatacaa gaaaattcaa agaagaccat
     2641 ctcaatactt ttagtcgtaa aaatgatagg ttagattggt cttattatcc acaagaacca
     2701 tccgctaacg atagcgaaaa taggaatgct tatcttcacg aattagggaa aattactaat
     2761 aggagtagat ttgtcgatgg tttaaatgtt gatgctcctt atactacaac tgctcactat
     2821 tggttaatta aacaaatggt taatgccgac gaatggcgaa tgattagtga tgatgacttc
     2881 tctattagaa acgcctttta tagagtattc acgaaagaat taaggttgag cgatgcacat
     2941 cattttattt gccaagttaa caagacgaaa agcagaaaac aatgcttaaa agaattcgga
     3001 caagcaaaag cagaactgct cgattgggga gatatcagag gattcaaaac aaagtttcta
     3061 cggactttgg catctcatta tctaactgaa ttactaacaa gtcatcaatt ccacgaagag
     3121 gctattagta aagatggaga aagatatcgc aaatatgccg ataaccctat caaacatcca
     3181 ttagccacta aggataaagg attttattca gttgattgta gaacggattt atctgctctg
     3241 gaaccaaacg aaatcgcaaa gatgctatta aatgttaatg accattcgac caatagcttt
     3301 attcaacaga taagacgtta tatatcatct cttgaaagac cattaacaac agctcgtggt
     3361 gataagaaga gctatattta cgctaacttc aatccgaagt atgctcaatt tgcgattaca
     3421 attctaagaa cttactacaa cttttgtcgc ccttttaaat cagcggataa aaaggttttg
     3481 acacctgctc aaagattagg gattacagat aaacaatttg attggaaaga tataatttat
     3541 ttcaaatgac aaaaagagct tggagtaatc ccaagctctt gtgctttatc tttactgttc
     3601 ttcttcaatt aacgcacccg ttaactcaat aagttttttc tttcatatac atgaaaatag
     3661 tagttatacg gatttttatc attctttatc cctttttggg caaatacctc attccattcc
     3721 tcataattca cttctggaaa aaaagtatct ccttcgaatt catgatgtat ttttgtgatg
     3781 tacattttct caacataagg gaaaaacaaa ttataaatct gttctcctcc gaaaataaaa
     3841 atttcttctt cgtttttaca taactcaaaa acatcttcta ttgaatgaac aatttcacaa
     3901 ccattaaagg taaacccctt atctctcgtc agaataatat ttcttctgtc aggtaaggct
     3961 cttccgattg attcaaggtt cttcctacct aatattatcg gatgtccctt tgtagtattt
     4021 ttaacatatt cccagtcctt gggaatcctc caaggaatgt cattctcttt gccaatcact
     4081 ctattcttat ccatcgcagc aatcaaagaa actttcattt ataacacagc ctttttaact
     4141 aatttaccat ctaatttctt atttctatca aaaaaccttt tctcattatc ctgcccgtta
     4201 gttgaataag caattttttc tataagttat attatcataa taccacaaaa tcacaaattt
     4261 tcattcaatt atcattttca attgcatttt ataaatcatt ttgtatgtaa tttggtatgt
     4321 aagttaaaat gaaaaaagta gtccattttg aagtacattt tgtcattttg tactccaaaa
     4381 ctgaactact tagtatagat tactgttgct tctttactaa tttaggttta cttgttaaga
     4441 aagattattt acgcaaatgt tcttcaacaa tttgctgaga tttatcagct gtatcttcca
     4501 cttcatcttt tgctttttct aacttttgaa tatattgttt caagcttgag ttttcagcag
     4561 ttgcttcgtc taatttcttt tgtgtatctg ttaactgagt ttctacattt gttttttctt
     4621 gtgtcagtgt tgcgatttgt tgattcaaac catttacagt attttgtaat tctgaaagtt
     4681 ttgcttgtgc ttcttgtagt cttgctgtcc aatctgcatt cagatgattg atttcatcat
     4741 ttttttggtt gatctcatct agtttgacat tgatttcatt ttgtttgctt tgtagttgtg
     4801 catccttgct gactaattga ctttctaagt tttgtttttc tgctgccgat tgattttttt
     4861 ggttttctaa ttcatttact tttgtttggt attcttggac atctgccttt gaatcttgca
     4921 atgtctgttc tttttgtgct agtcgagcgg taagatctgt aatcgcttgc tctttatctg
     4981 tcaatgtatg atcaagctgt gtcagattct cattgattgt atcaatatct gtctctccac
     5041 cccacaaaat aatttcattt gcgtaccgcg tgcctgtgta gccaattaga catagaaatg
     5101 caacgacaat cccaccagta ataaatttct ttttcatttg catagctcct taatccaata
     5161 cttttacatg ctaaaagtaa gtgatctcaa acacaatgtc aagcgttttt aaattacata
     5221 tgtaatttta atataaata
//
LOCUS       DACCYH010000135         5229 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.142, whole genome shotgun sequence.
ACCESSION   DACCYH010000135 DACCYH010000000
VERSION     DACCYH010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.142"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            114..1160
                     /locus_tag="IVY85_002766"
     CDS             114..1160
                     /locus_tag="IVY85_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8045718.1"
                     /translation="MSVKISVIVPCYNVEKYIDECINSLINQTLKDIEIILLNDGSTD
                     NTLAILKKYEKLDSRIKVYEHKNMGLGPTRNRGIKIANGEFLAFVDSDDFVSNTMFES
                     LYNCAIAENADIAIGEVGIFSDREIERVKIRKSLTNLKTIELATYNKKEFFKQFYFNR
                     LYSHNAWDKIYRRSLVLEFNLSFGDNKIIFAEDNWFQLQVFMTNTRICFVDKLCYYYR
                     QQNNSIMHKPKSNLVFRHGNMIADYEKLIRQNGNNIIDKKACMLVAFDVLIMEIFNQK
                     NVGGTCEDYKNALVSLKHHKILRENISNIVRMKAYELEPNMRKKLALVTISIMFNFKM
                     YKTAYNMLWRIYNR"
     gene            1174..2253
                     /locus_tag="IVY85_002767"
     CDS             1174..2253
                     /locus_tag="IVY85_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8045719.1"
                     /translation="MRKLTFVIGIMGKGGAERVIANLANNFIQDNWEIDIITIYGNRQ
                     DYELNRKIQVHPIICKSSFRAFRPLERFKELRKKIREIQPDLVISFLADVNIHVIISL
                     LGVKQKLIVSERNDPQRNPEEPWIRKIRDFLYKNVDGIVFQTPDAKKYFSKINDKKVK
                     TIIIPNPIKENLPYYNKTTSSKKFITACRLDKQKNLPLMIDAIGKLIHEGYECELDIY
                     GEGGLKTELESYINHNGLTEFVHLKEFTNKIHEKMQNAAGFVISSDYEGISNSMLEAL
                     AIGVPVIATDCPVGGARMYLGDRNEIGHLVECNNLDKLTDAMRRVLLDRESVVRKAQK
                     GIKLRKELTSKNISNIWYEYLNQFF"
     gene            2296..3696
                     /locus_tag="IVY85_002768"
     CDS             2296..3696
                     /locus_tag="IVY85_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HAP8045720.1"
                     /translation="MFSLTLVAFTIFCAFSLNQNYWLNWLGILIYLYAICTWHWRKGD
                     SIFSLYTIFFTLFLLFSYGQCIMWAFGIGTDRGIGTTVVAYGTGIIPSESDMIMVKWY
                     SCISMFVFHIGALLFTRKTTLRKVSWYESGDADADRKFLFKIGCIISIIVVPIVFVSK
                     LLEVRIALVHGYNALYYGDYATQSGYLQIILYLFFPALICLLIGSNFSKVITRFVFII
                     FALYSLLGIMSGDRGSWLYSSIILIWAYTQYKGTFNYKKLIKWLILAVIGIYILNVIT
                     KARDGGGLSKLTLKDFTSVFDSDDSPLVDSFFEMGGTMSIITFFLISGNGIYPFANTY
                     LTSLLGSISTRMLSMFGIKFVLLADWFSQDYLGITWGTGFSMIAEAYVNGGYIGGIVY
                     IFIIGIVIGKILSNSIDNQDIENHPMKMFLAITTANIIMGFPRAASYLIVKNFVYGVL
                     IILLINFFLQQFNKRL"
     gene            3742..>5229
                     /locus_tag="IVY85_002769"
     CDS             3742..>5229
                     /locus_tag="IVY85_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HAP8045721.1"
                     /translation="MERTARLKKNTIWALVFQITSIVCGMILLRLILTKYGSEVNGMI
                     SSINQFLQIISFLQLGVGAVMQSTLYKPLAEKDNLMISQILASGGKFYRQIAYILLVY
                     VGLLLAVYYVLVPNEFGRSYTMAMIATLSINSFAQYYFGIVNTLLLSADQKIYITYIA
                     QTIAFISNTVACALLISLNTEILMVKLITAIIYLACPIYYRWYVDRNYNIKRNVSYDK
                     EPIPQKWNGVAQHVASVVLDSTDVVVLSIFSNLIYVSIYNVYNLVIYGVKQLFMAMTN
                     GVQALLGELWVRKEIEVLYKTFAWFEWIVHTLTVFIFGCTGILIVPFIQVYTRGVTDA
                     NYNQLSFAALLVTANALHCLRTPYHVMIKAAGKYKETQNSFIIASLLNLGLSLILVKP
                     LGLVGVALGSLISITYHTLWSANYNSKYLLKWPMKKFYKQLFVNILTVVLSFGATSQL
                     KMMSITYLDWIVLAIKCAVIVFICIILVNVLFYRRYVVKIISKIIV"
BASE COUNT         1862 a          611 c          946 g         1810 t
ORIGIN      
        1 tgtaaatgca aatataaaga taattggaaa ggaattagtg ctaagtttgc attgaaaaac
       61 aaatgggtat ttttaattta tatttataac aaattgataa agaggtaata ataatgtcag
      121 ttaagattag tgtaattgta ccttgttata atgttgaaaa atatattgat gagtgtataa
      181 atagtttgat aaatcaaaca ttgaaagata ttgagattat tttgttaaac gatggttcaa
      241 cagataatac gttggcgata ttaaagaaat atgaaaagct tgattctaga ataaaagttt
      301 acgaacataa aaatatgggg ttagggccaa ctagaaatcg agggatcaaa atagctaatg
      361 gagaattttt ggcttttgtg gattctgatg attttgtttc caatactatg tttgaaagtt
      421 tatataattg tgcaattgca gagaatgcgg acattgcaat tggagaagta ggcatttttt
      481 ctgatagaga aatagaaaga gtaaaaataa gaaaaagtct aacgaactta aaaacaattg
      541 aattagcaac atataacaag aaagaatttt ttaagcaatt ctatttcaat cgtttatatt
      601 cacataatgc ttgggataag atatatagac gtagtttagt attagagttt aatttatcat
      661 ttggtgataa caaaataatt tttgcagaag ataattggtt tcaattacaa gtatttatga
      721 caaatacaag aatctgtttc gtagataaat tgtgttatta ttatcgccaa caaaataatt
      781 ctataatgca taagccaaaa agtaatttgg tttttagaca tggaaatatg attgctgatt
      841 atgaaaagtt gataaggcaa aatggaaaca acataataga taaaaaagct tgtatgttgg
      901 ttgcatttga tgtactaatt atggagattt ttaatcaaaa aaacgttggt ggaacatgtg
      961 aagattataa gaatgcatta gtttctttga aacatcataa aatattgaga gaaaatattt
     1021 caaatatagt tagaatgaaa gcatatgaac ttgaacctaa tatgagaaag aagttagccc
     1081 ttgtgaccat tagtataatg tttaatttta aaatgtataa aacagcgtat aatatgctct
     1141 ggagaatata taatcgataa agaggtagga aatatgagga aattgacctt tgtgatagga
     1201 attatgggaa aaggaggggc agaacgagta attgcgaatt tggcaaataa ttttattcaa
     1261 gataactggg aaattgatat tataactata tatgggaatc ggcaagacta tgaactgaat
     1321 agaaaaatac aggtacatcc aataatatgt aaaagttcgt ttcgtgcatt tagacctttg
     1381 gaacgcttta aggaacttag gaaaaaaata agggaaatac agcctgatct ggttatttcg
     1441 ttccttgctg atgtaaatat ccatgtcatt atttcattgt taggagtaaa acaaaaatta
     1501 attgtttctg aacgaaatga tccacaacgg aatccagaag aaccatggat taggaaaatt
     1561 agagattttc tttataaaaa tgtagatgga attgtttttc aaacaccaga cgcaaaaaaa
     1621 tattttagta agatcaatga taaaaaagta aaaacaatta taattcccaa cccaataaag
     1681 gaaaatttac cttattataa taaaactaca agtagtaaaa aatttattac tgcatgtaga
     1741 ttggataaac aaaaaaattt gcccttaatg attgatgcaa taggaaaact tattcatgaa
     1801 ggatatgagt gtgaactgga tatttatgga gaagggggat taaaaaccga gcttgaaagt
     1861 tatatcaacc ataatggatt aacagaattt gttcatctaa aagaatttac aaataaaata
     1921 catgaaaaga tgcaaaatgc ggcaggattt gtaatttctt ctgattatga aggtatatct
     1981 aattctatgt tagaagcact ggctattgga gtgccagtta tcgctacaga ttgtccagta
     2041 ggtggcgcaa gaatgtattt aggtgataga aatgaaatag gtcatcttgt tgaatgtaat
     2101 aatctagata agttaacaga tgcaatgcgt agggtacttt tggatagaga aagtgtggta
     2161 agaaaagcgc aaaaaggaat taaactaaga aaagaactga catctaaaaa tatttcaaat
     2221 atatggtacg aatatttaaa ccagttcttt taaccaaaag gaggatattg tgtgaaagaa
     2281 aaaataaata tattgatgtt ttcgttaacg ttagtcgcgt ttacaatatt ttgcgctttt
     2341 tcattaaatc agaactattg gctaaattgg cttggaatat tgatttactt atatgcaatc
     2401 tgtacatggc attggagaaa aggagattca atattttcat tatacacgat ttttttcact
     2461 ctttttttat tatttagtta tgggcaatgc attatgtggg cattcggtat tggaacagat
     2521 cgtggaatcg gtacaacagt agtagcttat ggtacgggga ttattcctag tgaatcagat
     2581 atgataatgg taaaatggta ttcttgtatt tctatgtttg tatttcacat aggagcactc
     2641 ttatttacga gaaaaaccac actaagaaaa gttagttggt atgaatctgg agatgctgat
     2701 gccgatagaa agtttttatt taaaataggg tgtatcattt ctataatagt agtaccgata
     2761 gtttttgttt ctaaattact agaggtacgt atagcattag tccatggtta taatgcatta
     2821 tattatggtg attatgctac acaaagtgga tatttacaaa ttattctata tctttttttt
     2881 ccagccttaa tatgtttatt aattggtagc aacttttcaa aagtaataac gagatttgtt
     2941 tttattattt ttgctttata ttctttatta ggaattatgt caggagacag aggaagttgg
     3001 ttgtatagct ctattatact catatgggca tacactcaat ataaaggaac ctttaattat
     3061 aaaaaattaa taaagtggct tattttagct gttataggaa tatatattct aaacgtaata
     3121 acgaaagcac gtgatggtgg aggactaagt aaacttactt taaaagattt tacatctgta
     3181 tttgattcag atgattctcc tttagttgat tctttttttg aaatgggggg gactatgagt
     3241 attataactt ttttcctaat aagtggtaat ggaatatatc cctttgctaa tacttatttg
     3301 acctcactct taggctctat atcaacaaga atgctatcaa tgtttggaat aaagtttgtt
     3361 ttattagctg attggttttc tcaggattat ttaggaatca cttgggggac tggattctca
     3421 atgattgcag aagcctatgt caatggtgga tacattgggg gtatagtcta tatttttatt
     3481 attggtatag tcatcggtaa aatcctttct aatagtatag acaaccaaga tattgaaaat
     3541 catcctatga agatgttttt ggctataact acagcaaata ttataatggg ttttcctaga
     3601 gctgcttcat atttaattgt gaaaaatttt gtgtatggtg ttttaattat attgttgata
     3661 aactttttcc tacagcaatt taataaacgt ctttaatatt taagcttaat aaaaagataa
     3721 aacaagtcag gagttgcaaa aatggaaaga actgctcggc tcaaaaaaaa cactatttgg
     3781 gctctggtgt ttcaaataac ttctattgtc tgtggtatga tcttgctacg tttgatatta
     3841 acaaagtacg gatccgaggt taatggaatg attagctcta ttaaccaatt tctacaaatt
     3901 atttcttttt tacaattagg agtaggcgct gtaatgcaat caactctata caaaccacta
     3961 gcagaaaagg ataatttaat gataagtcaa atcttagctt ctggagggaa gttttatcgg
     4021 caaattgcat atattctact agtatacgtg ggtttgttac ttgcagtcta ttatgttctc
     4081 gttcctaatg aatttgggag atcatataca atggcaatga ttgcaacatt aagtattaat
     4141 tcatttgctc aatattattt tggtattgtt aatactttgt tattaagtgc tgaccagaaa
     4201 atttatatta cgtatattgc acagacaata gcatttattt ctaatacagt tgcatgtgct
     4261 ttattaatta gtctgaacac agagatcctt atggtaaaac ttattactgc tataatttat
     4321 cttgcttgtc caatatatta tagatggtat gtggatcgaa actataatat aaagaggaat
     4381 gtgtcatatg ataaggaacc gatccctcaa aaatggaatg gggttgcaca acacgtagct
     4441 tctgttgtac tagatagtac tgatgttgta gtgttatcta tattttctaa cctgatatat
     4501 gtatctatat ataatgtgta caatttagta atatacggag taaaacagct attcatggct
     4561 atgacaaatg gcgttcaggc cttactaggg gaactttggg tcagaaaaga aatagaagtt
     4621 ctttacaaaa catttgcatg gttcgaatgg attgtacata ctttgacagt ttttattttt
     4681 ggctgtaccg gtatactaat cgttccattt attcaagtat atacaagagg agtaacagat
     4741 gctaattata atcaattatc attcgcagct ttacttgtaa ctgctaacgc acttcactgt
     4801 ctgcgtacac cttatcatgt aatgataaaa gcagctggta aatataaaga aactcaaaat
     4861 agttttataa ttgcttcttt gttaaattta ggtttatcat taattttggt taaaccacta
     4921 gggctagttg gagtagcatt aggatctcta atttcaatca catatcatac actttggagt
     4981 gcaaattata attcaaaata tttgcttaaa tggccgatga aaaaattcta taaacagtta
     5041 tttgtaaaca tcttaactgt agttctttct tttggggcga catcacaatt gaaaatgatg
     5101 tcaatcacat atttggattg gattgttctc gcaattaaat gtgcagttat tgtatttata
     5161 tgtataatcc tagttaatgt tttattttat agaagatatg tagtaaaaat aattagtaaa
     5221 atcatcgta
//
LOCUS       DACCYH010000136         5135 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.143, whole genome shotgun sequence.
ACCESSION   DACCYH010000136 DACCYH010000000
VERSION     DACCYH010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.143"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            45..1334
                     /locus_tag="IVY85_002770"
     CDS             45..1334
                     /locus_tag="IVY85_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8045722.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQ
                     LPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(1460..2539)
                     /locus_tag="IVY85_002771"
     CDS             complement(1460..2539)
                     /locus_tag="IVY85_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8045723.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2648..3088)
                     /locus_tag="IVY85_002772"
     CDS             complement(2648..3088)
                     /locus_tag="IVY85_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8045724.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3145..3555)
                     /locus_tag="IVY85_002773"
     CDS             complement(3145..3555)
                     /locus_tag="IVY85_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8045725.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3736..4170)
                     /locus_tag="IVY85_002774"
     CDS             complement(3736..4170)
                     /locus_tag="IVY85_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8045726.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(4270..5118)
                     /locus_tag="IVY85_002775"
     CDS             complement(4270..5118)
                     /locus_tag="IVY85_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8045727.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         1886 a          993 c          871 g         1385 t
ORIGIN      
        1 tatcttattt cttgcataca aaaaaatcaa ggagtgagag agttatgaga tattcaacca
       61 tcttaatatc aacatttgca gccttaagct tatcgcttac tataaatcag acagtccaca
      121 cagaggagat agaaacagcc aaatggacag aaaccaagtg ggccaacgtc aatcgaatta
      181 cctttcagga tgaattttca tccttcaatt caggagtcgc aaaattaaga aaaggacaaa
      241 cagcgaccat caaagcaaaa attgactatt caggagactt agcacctata ctacaatcag
      301 ctgttacatt tgagaatgtt gatcccgctt tgtcgattgg ctatgacgag aacactttga
      361 ctatccaaca caagcaagcg atatttacct tcaccgttac attaaatcag gacttgttaa
      421 aaccagcgct ttttactgtc aaggtatcag atggactacc gaacgatcat catcatttaa
      481 cgccgtatag tcagcggcag acaattgagc aagattcagc tatcgactca ggaggagatc
      541 ttgttgaaga accaacagat aaaccagaaa atgaaaataa gccagaagta ccgcctacag
      601 aaaatccaga cggagaacag aaaccggaaa tagaacctgg agaagaaccc gatacagaaa
      661 ctcaaccaga gccagacaat gaatcaaaac ctgaaataac ccctggagaa aaaccagatg
      721 tagacccaga agaaaaaccg gatgtgactc cagaaccaga tacggattct ggaaaccaaa
      781 cagtaccgga aacaaatcca gacacagata atgaaactga aaatccagaa aaaccagagg
      841 tagccccaga agaaaaacca gatgtaactc cagaaccaga tacggattct ggaaaccaaa
      901 cagtaccgga aacaaatcca gacacagata atgaaactga aaatccagaa aaactagagg
      961 tagatccaga agaaaaacca gatgtgactc cagaaccaga tacggacgca agagaccaag
     1021 gaataccaga aaaaataaac aaaaaaacaa ttcaagagga tggaaaaaaa gagagtaaaa
     1081 aatcaaatct agcaattcta aaaataaatg aagagcagct gaataaaaaa tcacgaatat
     1141 ttgatagcgc ccaatcagct gaaaccttaa aaagtagcaa ggatacaacc tttgctagtc
     1201 ctgaaacgaa aaataaacag ctaccaaaat ccggagagtc acaaaacaaa gttatactgt
     1261 ggagcggaat cattctatta agtatagcaa caatgctttc tgcaaaaaga tttaaacaaa
     1321 acagatctct ctaacatttg aataatgtct gtgaacgcat ttttcaaata aaaagaaata
     1381 tccagaaaac gaccccaaaa taccagattg aaatctgata tttggggtcg ttcatacttt
     1441 atctttcttc atatttattt taagggatat ctgtttcttt tggaagatgg ttcgggatct
     1501 ttttcacttt caatacatct aaaaagaagg taaagatcgg aatgccgaca atcaagcccc
     1561 aaacgccaaa gaaccgttcg ctaacaagta agaccacaaa tgtataaaaa atcggcagtt
     1621 ctgttttgct ggacatcaac ttcggattca gcacataaga ctcaattaga tggacaatca
     1681 aaatcgtaat caggatataa acaacatctc ggatgcctcc ttgggaataa gctatgaaag
     1741 atagaggaat acaagaaatg ataacacctg ctactgggat cagacttaaa atgaaaatca
     1801 tgatcgctaa actcagcagc tgatgaaagc caaatacagc caaggcgatt gtagtgagca
     1861 acgtgtttaa aagagcgata acaaattgag cttctaatac caaaccaaat gtattgacaa
     1921 acttatcggc aaaatagtaa atatcttgaa agaaccaaga aaattcactt tttaaaaaca
     1981 atcttgagaa tacaattgtt tttttctttt caatcataaa aaagaaactt aagatgaatg
     2041 acatagcaaa cgccactgcc atttttccta tatcctgtaa atagccaagc gcaatcgaag
     2101 cgccattttg aagctgttca aaaaaatcgt ttcgctcaat ataagtatgg atatattgta
     2161 aaacaggatc tgcatctttt ggctgatgct cgtaaaaatc aataacggac tggatcatat
     2221 caaatgtttg atgaataagc atcgggatat actttgtgaa tgccagatag ataaggtaaa
     2281 cgatcagtgt ataaatcatg atagctaaaa cacctgtagg gattcggaca aatcgctgaa
     2341 tgatttgaac aagatgaatc atcaaataag taaagataaa tgtgagcaaa atggttgtaa
     2401 tcatacttct aactaagaaa agaacaaaaa cgacaaataa taaaacagtg agacggcgta
     2461 ttttttcatt cgccaaaaaa cgctgataaa cggtcaaatc gacaacctcc aaataacaaa
     2521 aaataaacac gttattcatt atatgatgga atatgaaaat aagaaagaga aagagcaaaa
     2581 agaaaaaccg ctaaatgacg ttatcttatg agttggtttt cctcataaaa tcgttcatct
     2641 agcagtttca gccctttttc tggtcttgat gaaaattatc tagcattttt cgggtcagat
     2701 gttgcagtgt atccaattct tcctgttcaa gtggaagata agacttgatc ttttgtggaa
     2761 taccttctgc ttgtttttgc aacgcagcac cttgaggagt cagcgtaaca tttacgattc
     2821 tttcatcacc cgtttcacgc tgacgattaa tataattttt ttctgctaac ttttttagta
     2881 agggagtcag cgtaccagaa tccagacaca aatcatttcc tagctctttc actgaagtct
     2941 ttttcttttc ccataaaact aacatcacaa gatactgggt ataggtaaga ttcagcggct
     3001 tgaggaggga agtgtagtgt ctaatgattt ccttagaggc ggcatataaa ggaaaacata
     3061 cttgattaac taaatataga tcttccattt ttccacctcc gctgttttac tatagtgtat
     3121 catagcggat ttacacaaaa aagtctattc actaactgct ttcagagtaa cttctatatt
     3181 tccagctgta gcttttgaat acggacacac tttatgcgct gcctctaata attcttgcgt
     3241 ttcttccaga gaaagacctt cgatagatcc ttcaatctct acacctaaga caaagctcgt
     3301 ttcactttct gaataaaggg aaacttgtgc actgacagta gaagccccag agatattttt
     3361 ttgctgtttg atcaattcta atgcactgtt aaaacatgca ctgaaaccag ctgcaaacaa
     3421 ttgttcaggg ttggttgcat tttcgacgcg tttaccagga gctgcaatat tcaattcgaa
     3481 tgtacgatcg ggattatgaa cttctccaga gcgtccgcct gtgttgatca ttttcgtaga
     3541 atacattttt ttcataaaat taccaccttt aattatattt tatacaattg aattttacac
     3601 aatataattg tggaaagcaa agaaatggat tggcataaga taaataagag aaagcacatg
     3661 aaacagacgc gctttctcga atagcaaaca tatacaaaaa aatatcagag acaaaaacta
     3721 atctcatcat aaacattaca tatatttctc aaaaagttgt tttctttctt caaaacttac
     3781 ttcacgatgg acctgatgaa gcatccataa ataaccaaaa ggatctctga acattgagtt
     3841 agtcacacca aagtccatca tttctgtcat ttcttgaatc tctacacagc catttcgtaa
     3901 tgctttatta tgtgtgtcct cgatatcaga gacgatgata ttgaaccaga tcgtatttgg
     3961 atgttcagca gtaggagcag tcaaaccaag agacgtgttt tcatccagca tatggaatct
     4021 cgtgccatac aaggtaaaaa tgacttcatt attgcctcta gggtaatctg ttgcctcaat
     4081 cagttgtatt tcatcaaaga ttgtttgata cagttctagt gctttcaaac tatcatttaa
     4141 tacaaaatct atttccgttc ctttatgcat aaagatctcc tctccttttc agtcgaacag
     4201 ttactagatt cttttattat accagttaaa gaaaacgcat tcaaaaaaat agcggattta
     4261 cttaccaact cattcgattg gatgataagt aaatccgcgt cccattactt ctgctacgct
     4321 gctgacaatg acaaaggcac ttggatcgat ttcttctatc aatggtttca ttcgggaaaa
     4381 ttcatgatca gtcacaacga tcaataaaac ctcttttgac tttcgattat acccgccttt
     4441 tgcctctagc agggtaaggc cacgtccaat ctcagcactt aagcgaaggc ggatgtcttc
     4501 caaataatgt tcactcatga ccataacaga tttctttcga ttgatacctg tttcgatata
     4561 agtcatgaca attgaagtga ttgcaataga taaaatagca taaagaaact cttctattcc
     4621 aaaaacaaac agattaagag aaacaacgaa tgcgtctgta aaaaataagc caatcgaagt
     4681 attcaagtgg aaatattttt taaaaatgag gggagggatc gtcgtaccgc cgctagaaga
     4741 gttgttttta tataaaatcg caactcctaa agcaaaaatc gcacttccaa aaatgacaga
     4801 caacaaccga tctgaagtaa tcattgtttc tggaactatt gcaatgatta aaggaaaaaa
     4861 caagcttccg aaaagtactt taaaaaaggg ttttttacct aaaaatagaa gagctagtac
     4921 caacataacg atattcaaca ctaaaataac tatagctcgg tcaaatccta aaacagcttc
     4981 cagtaatatt cctagaccgc tgacacctcc agcagcaata tgatgaggcc ctaaaaacat
     5041 attgatagcg actcctaata tgaagagggc aaaaataatc ttaatgtatg tccatataaa
     5101 ttgtctcgtt tctttcattt atccacttcc ttaat
//
LOCUS       DACCYH010000137         5034 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.144, whole genome shotgun sequence.
ACCESSION   DACCYH010000137 DACCYH010000000
VERSION     DACCYH010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.144"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IVY85_002776"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IVY85_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP8045728.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IVY85_002777"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IVY85_002777"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP8045729.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IVY85_002778"
     CDS             complement(2169..2906)
                     /locus_tag="IVY85_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8045730.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IVY85_002779"
     CDS             complement(2997..3524)
                     /locus_tag="IVY85_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP8045731.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IVY85_002780"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IVY85_002780"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP8045732.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IVY85_002781"
     CDS             complement(4401..4886)
                     /locus_tag="IVY85_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP8045733.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
BASE COUNT         1337 a         1125 c          862 g         1710 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acct
//
LOCUS       DACCYH010000138         4931 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.145, whole genome shotgun sequence.
ACCESSION   DACCYH010000138 DACCYH010000000
VERSION     DACCYH010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.145"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            83..970
                     /locus_tag="IVY85_002782"
     CDS             83..970
                     /locus_tag="IVY85_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8045734.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="IVY85_002783"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="IVY85_002783"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP8045735.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="IVY85_002784"
     CDS             2272..2718
                     /locus_tag="IVY85_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8045736.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="IVY85_002785"
     CDS             2991..4343
                     /locus_tag="IVY85_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP8045737.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="IVY85_002786"
     CDS             4514..4801
                     /locus_tag="IVY85_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045738.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1693 a          813 c         1030 g         1395 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaac cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt t
//
LOCUS       DACCYH010000139         4776 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.147, whole genome shotgun sequence.
ACCESSION   DACCYH010000139 DACCYH010000000
VERSION     DACCYH010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.147"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(207..1169)
                     /locus_tag="IVY85_002787"
     CDS             complement(207..1169)
                     /locus_tag="IVY85_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8045739.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="IVY85_002788"
     CDS             complement(1171..2610)
                     /locus_tag="IVY85_002788"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP8045740.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="IVY85_002789"
     CDS             2795..4741
                     /locus_tag="IVY85_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP8045741.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1516 a          819 c          790 g         1651 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaggc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttc
//
LOCUS       DACCYH010000140         4738 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.148, whole genome shotgun sequence.
ACCESSION   DACCYH010000140 DACCYH010000000
VERSION     DACCYH010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.148"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            150..956
                     /locus_tag="IVY85_002790"
     CDS             150..956
                     /locus_tag="IVY85_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HAP8045742.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            974..1801
                     /gene="vanW"
                     /locus_tag="IVY85_002791"
     CDS             974..1801
                     /gene="vanW"
                     /locus_tag="IVY85_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAP8045743.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVFADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            1798..2769
                     /locus_tag="IVY85_002792"
     CDS             1798..2769
                     /locus_tag="IVY85_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP8045744.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2762..3790
                     /gene="vanB"
                     /locus_tag="IVY85_002793"
     CDS             2762..3790
                     /gene="vanB"
                     /locus_tag="IVY85_002793"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP8045745.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            3796..4404
                     /locus_tag="IVY85_002794"
     CDS             3796..4404
                     /locus_tag="IVY85_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP8045746.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT         1331 a         1003 c         1262 g         1142 t
ORIGIN      
        1 ttgaccgaca ggtataaccc tgccggtctt tttttgctgc tacaggaaaa ctacagactg
       61 gctacaggga aagtacagat gcgcttgcca taataacaat cgtaccagcc acaaatcgta
      121 gttttattgc aaaggaggca ttcgatcaaa tggaaaaaag caactatcat tccaatgcgg
      181 atcatcacaa acggcatatg aaacaatccg tggaaaagcg ggcttttcta tgtgcgttca
      241 ttatttcgtt cacagtctgc acgctgtttt tggggtggag actggcttcc gtattggagg
      301 caacacagat accgcccatc cctgcaactc atacaggcag cagcactgac gtagtggaga
      361 atttggagga aaacgctctt gccaccgcca aagaacaggg agatgaacag gaatggagcc
      421 tgattttagt gaacaggcag aaccccatcc ccgcacagta cgatgtggag cttgagcaac
      481 tatcaaatgg tgagcggata gatattcgga tttctcccta tcttcaagat ttgtttgatg
      541 ccgcaagaac tgatggagtt tacccgattg tcgcatccgg ataccgaaca acagaaaaac
      601 agcaagaaat tatggatgaa aaaattgccg aatataaggc gaaaggctac acctctgcac
      661 aggctaaagc ggaagcagaa acttgggtgg ccgtgccggg aacgagcgag catcagcttg
      721 gtcttgctgt ggatatcaat gcggacggaa ttcattcaac aggcaacgag gtttatagat
      781 ggctggatga aaacagctat cgttttggtt ttattcgtcg ctacccacca gacaagacag
      841 agataaccgg tgtgagcaac gagccgtggc attaccgata tgttggcatc gaagctgcca
      901 cagagatgta caaccaaggg gtttgccttg aggaatattt aaaaccagaa aaatgagaaa
      961 aggatgttat gttatggaca gaaaaagatt gacacagcgt ttcccgttcc tgcttccaat
     1021 gagacgggcg cagagaaaaa tgtgctttta tgcgggaatg agatttgacg gctgtcgcta
     1081 tgcgcagacg ataggggaaa aatcgctttc ccatttgctc tttgaaacgg attgcgcatt
     1141 atacaaccac aataccggat ttgacatgat ataccaggaa aacaaggtgt tcaacttaaa
     1201 gctggcagca aagaccttaa acggcttatt gataagaccg ggggaaacct tttctttctg
     1261 gtggctggta cgccatgcgg acaaagatac cccctataaa gacggcctta cggtgaccaa
     1321 tggtaaactc accaccatgt cgggcggcgg tatgtgccag atgagcaatt tactattttg
     1381 gatgttcctg catacgccat tgacaattat ccagcgcaga ggtcacgaag taaaggagtt
     1441 tccagagcca aacagtgacg agattaaagg ggtggatgca accatttcag agggctggat
     1501 tgatttaaaa gtgcgaaacg ataccgactg cacctaccaa atatgggtga ccttagatga
     1561 tgagaaaatc atcggtcagg tgttcgccga caaagagccc caagcattat acaaaattac
     1621 aaatggcagt atccagtatg tccgtgaaag tggcgggatt tatgaatatg cccaggttaa
     1681 acggatgcaa gttgccttag gtaccgggga aataatagat tgcaagctgc tttatacaaa
     1741 caaatgcaaa atctgttatc ccctcccgga aagtgtggat attcaggagg agaaccaatg
     1801 agaaaaagtg tgggcattac tgtttttgga tgcgaacagg atgaggcaaa tgctttccgc
     1861 gctttatcgc cggattttca tattatccct acgctgatta gcgatgcgat atcggcagac
     1921 aacgcaaaat tggccgctgg caatcaatgc gttagcgtaa gccataagtc cgaggtttcc
     1981 gaggcgacaa ttcttgcgct gagaaaggtc ggggtaaaat acatttctac ccgcagcatc
     2041 ggctgcgatc acattgatac gactgccgcc gagagaatgg gaatctcggt tggcacggtt
     2101 gcgtattcgc cggacagcgt tgcggattat gctttgatgc tgatgctgat ggccatacgg
     2161 ggtgcaaaac ccaccatgca cgccgtggcg caacaagatt tcagattgga tcgtatccgg
     2221 gggaaagaac tgggggatat gactgtggga gttattggaa ccggccatat cgggcaagcg
     2281 gtcgtcaaaa ggctgcgggg atttggatgc catgtgctgg cctatgataa cagccgaaaa
     2341 atggatgcag attatgtcca gcttgatgag cttctaaaaa acagcgatat tgttacgctc
     2401 catgtgccgc tttgtgcgga tacccgccat ctgatcggtc agaagcaaat tggagagatg
     2461 aagcaaggcg catttttaat caacaccggg cgcggagcgc ttgtcgatac cggggcgctg
     2521 gtggaggcgt tggaaagcgg aaagctgggt ggtgcggcac tggatgtgtt ggagggcgag
     2581 gatcagtttg tttataccga ttgctcgcag aaagtgcttg accatccctt tttgtcgcag
     2641 cttttaagga tgccgaatgt gatcatcaca ccccatacgg cgtactacac tgagcgtgtg
     2701 ctgcaggata ctacagaaaa aacaatcagg aattgtctca attttgaaag gagtttacag
     2761 catgaataga ataaaagtcg caatcatctt cggcggttgc tcggaggaac atgatgtgtc
     2821 ggtaaaagcc gcaatagaaa ttgctgcgaa cattgatacg gaaaaattcg atccgcacta
     2881 catcggaatt acaaaaaacg gtgtatggaa gctatgcaag aagccatgta cggaatggga
     2941 agccgacagt ctccccgcca tactctcccc ggataggaaa acgcatgggc tgcttgtcat
     3001 gaaagaaagc gaatacgaaa cacggcgtat tgatgtggct ttcccggttt tgcatggcaa
     3061 atgcggggag gatggtgcga tacaggggct gtttgtattg tctggtatcc cctatgtggg
     3121 ctgtgatatt caaagctccg cagcttgcat ggacaaatca ctggcctaca ttcttacaaa
     3181 aaatgcgggc atcgccgttc ccgaatttca aatgattgat aaaggtgaca agccggaggc
     3241 gggtgcgctt acctaccctg tctttgtgaa gccggcacgg tcaggttcgt cctttggcgt
     3301 aaccaaagta aacggtacgg aagaacttaa cgctgcgata gaagcggcag gacaatatga
     3361 tggaaaaatc ttaattgagc aagcgatttc gggctgtgag gtcgggtgtg cggtcatggg
     3421 gaacgaggat gatttgattg tcggcgaagt ggatcaaatc cggctgagcc acggtatctt
     3481 ccgcatccat caggaaaacg agccggaaaa aggctcagaa aatgcgatga ttacagttcc
     3541 cgcagacatt ccggtcgagg aacgaaatcg ggtgcaggaa acggcaaaga aagtatatcg
     3601 ggtgcttgga tgcagagggc ttgcccgtgt tgatcttttt ttgcaggagg atggcggcat
     3661 cgttctaaat gaggtcaata ccctgcccgg ttttacatcg tacagccgct acccacgtat
     3721 gatggccgcc gcaggaatca cgcttcctgc actgattgac agcctgatta cattggcgtt
     3781 aaagaggtga taagtatgga aaatggtttt ttgtttttag atgaaatgtt gcatggcgtc
     3841 cgttgggatg ccaagtacgc cacatgggat aacttcacag gaaaaccggt ggatgggtat
     3901 gaggtgaatc gcatcatcgg cacaaaggcc gtggcgtttg ctctgcgcga ggcacaaatc
     3961 catgcggctg cgcttggcta tggcttgctt ttatgggatg gatatcggcc aagaactgcg
     4021 gtggactgct tcctgcgttg ggcagcgcaa ccggaggaca atctcacaaa agaaaaattt
     4081 taccccaata tagagcgagc cgagttgatt acaaagggtt atgtggcctc acaatccagc
     4141 catagccgtg gaagcgcaat tgatcttacg ctctaccacc tggatacagg ggaacttgtt
     4201 tcaatgggaa gtaacttcga ttttatggac gaacggtcgc atcatacagc aaaagggata
     4261 ggggatgcag aggcacaaaa tcgaagatgc ttgcgtaaaa tcatggaaag cagcggattt
     4321 cagtcttatc gctttgaatg gtggcactat aagttgattg atgagccata ccccgatacc
     4381 tattttaatt ttgccgtttc ataatgaaag tattggtttt gtatcagatt ttggatttga
     4441 taaaatctat gctggtcatg gacaatcttt tactggaaag tggttaaagc atcttttgta
     4501 atacattgca aattatagta ttcaatctgc ccagcggcag aaaagaaaaa aaccgctgtt
     4561 gggcagacct ttcccacgcc cattttgtat tttgctggcg gcggttttga gaaatcaagg
     4621 ccgccgttct tttctggcag caaaaggtgt ggcggcccat aggaggatgc cgctatgctg
     4681 ttttgctatt gccatgctct aatccacttc caccactcaa aaaaagcctt gcccctcc
//
LOCUS       DACCYH010000141         4698 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.149, whole genome shotgun sequence.
ACCESSION   DACCYH010000141 DACCYH010000000
VERSION     DACCYH010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.149"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            59..913
                     /locus_tag="IVY85_002795"
     CDS             59..913
                     /locus_tag="IVY85_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8045747.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="IVY85_002796"
     CDS             1010..3259
                     /locus_tag="IVY85_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8045748.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..4437
                     /locus_tag="IVY85_002797"
     CDS             3262..4437
                     /locus_tag="IVY85_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8045749.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRVTRGAS"
     gene            complement(4440..>4698)
                     /locus_tag="IVY85_002798"
     CDS             complement(4440..>4698)
                     /locus_tag="IVY85_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP8045750.1"
                     /translation="GITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQN
                     FNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1638 a          678 c          854 g         1528 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga tatcggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acgggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actagagtaa cccgtggtgc tagttaattt
     4441 taatatccta aagaatatct caaagttcct tcaaaacgtc gttgtgctcg acttaataga
     4501 acactgtaag ccagtaatat gctttcaaat ctactctcta gccctttaac agaacgtgag
     4561 ttgaaatttt gacaacctaa tttttctaag gaagaaaaaa cagtctcaac tgtttttctt
     4621 tgttttccaa gtaagctgtg gtctagtttt tctgatttat ccatgttttt acgtggcgga
     4681 acggatatag taatgccc
//
LOCUS       DACCYH010000142         4643 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.150, whole genome shotgun sequence.
ACCESSION   DACCYH010000142 DACCYH010000000
VERSION     DACCYH010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.150"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(52..522)
                     /locus_tag="IVY85_002799"
     CDS             complement(52..522)
                     /locus_tag="IVY85_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP8045751.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="IVY85_002800"
     CDS             complement(681..1139)
                     /locus_tag="IVY85_002800"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP8045752.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="IVY85_002801"
     CDS             complement(1310..2455)
                     /locus_tag="IVY85_002801"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP8045753.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="IVY85_002802"
     CDS             complement(2468..3385)
                     /locus_tag="IVY85_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP8045754.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="IVY85_002803"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="IVY85_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP8045755.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1455 a          847 c          876 g         1465 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaagcgaaaa tattttttca tca
//
LOCUS       DACCYH010000143         4586 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.151, whole genome shotgun sequence.
ACCESSION   DACCYH010000143 DACCYH010000000
VERSION     DACCYH010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.151"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            185..2854
                     /locus_tag="IVY85_002804"
     CDS             185..2854
                     /locus_tag="IVY85_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8045756.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2945..3565)
                     /locus_tag="IVY85_002805"
     CDS             complement(2945..3565)
                     /locus_tag="IVY85_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8045757.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3679..4218
                     /locus_tag="IVY85_002806"
     CDS             3679..4218
                     /locus_tag="IVY85_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045758.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4215..4553
                     /locus_tag="IVY85_002807"
     CDS             4215..4553
                     /locus_tag="IVY85_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HAP8045759.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1418 a          790 c          860 g         1518 t
ORIGIN      
        1 tttttaaggg tgggactttt gattcctacc ctttttttgc gtttattttt gttatacgaa
       61 caaattatga agttatttat tctaaatttt atttttttgt gaaaattaga ctctttttaa
      121 gcagagaaaa acaaggatat gataaactga atacaacttc atagaaattt ggtggaaaat
      181 aaatatgaaa aaaaatttaa agtcattctt tagagaaaac ggacgctacg cagcagctag
      241 tttcctgatt ccgtttttga ttttagcact tgtttatcta agtatcggga tctatcctgg
      301 aagcagcaga agtattttgg caagtgatgc cttttcacag ttttctaatt ttcatgccag
      361 cttccgaaat gttcttcttg gaaagcaaag tattttttat acatggaatg cttctctagg
      421 attgaattat ttatctttaa tttcttacta tctaggcggg atcttcacgc cactggtgtt
      481 cttttttcca aaccagatga tgccagatgc actgtatgtt ctgactttgc ttaaggtcgg
      541 ctgtgccgga ttggcttttt ggtttcttgc acgtacgttc aagattcctc gctgggggca
      601 tgttgcactt agcgtcagtt acgcattgat ctcatttatc actgcacatt cggagatcat
      661 catgtggctg gatgcatttg tttatcttcc attagtgatt ttggggattc atcgtgttat
      721 ggatcttaaa cggccgaaac ttttatttat cagttattta ctcttgtttt tatccagttt
      781 ttatatgggt tttatgattg gcgtattttc tttcttatat tttatcgccc gtctattgac
      841 aaactggtca gaatataaaa aaacgattgt tccttatggt atcacttctt tactggcagg
      901 cggcgcttca atgatcattg tattgccagc agtgctggat cttcggtcaa atggcgaggc
      961 actgagtcag atcactacgt tgaaaacaga agcgacttct tatctggatc taatcatgaa
     1021 aaatatgatt ggtgtttatg acacgacaaa atacggatcg attccgttcg tttacgcagg
     1081 tctgctacca ttagtcctct gtctgtttta ttttgccagt cggaaagtac cgttgaaaaa
     1141 taaatttttg tacggcagtc ttttcgccat acttatcgca agtttctatc ttgttccatt
     1201 gaatttgttc tggcatggta tgcatgcacc gaatatgttt ttattccgtt atgcctatct
     1261 gttctctttc ttagtgctcc tgcttgcagg aaaaggatgg gaaaagatag aacaagacga
     1321 tcgtgggctg ttgttaggaa tcattttggt cgtagctgct ctctttgcgg tggcatgggg
     1381 catcaaacct gaaggaagct acacctatgt gacgatgact tcctttgtat tgacagtggt
     1441 tttcttaggg atttatgctt ttgctgtggg attttatcag cataaaaaac tttcattcaa
     1501 tcatctgagt atattgttat tattgatcat gtctgctgaa gcactggtca atacgaacag
     1561 tatgatccat ggcattcttg atgattggaa ttatgcttcc cgcagtttat atactgatcc
     1621 ttatcctgac ttgaaagctc ttgtaaatga aacaaaaaaa gattccgact cgttttaccg
     1681 cttggaaaat ctggatccag tatctgcaaa cgattcgatc aactatggtt acagtggtat
     1741 cagtttgttt tcttctataa gaaatcgtaa ttcttcctct tacctggata aattaggttt
     1801 ccgttcaaga gggacaaatt tgaatatccg ttataacaat aatactttgc taatggatgg
     1861 gtttacagga atcaaatata acatttctaa aaacgaaagc acgttcagta aatatggctt
     1921 ccgccaagct ggtcaaagtg gcgactatac actgtatcag aatgtcaatg cattgccact
     1981 agcatttact gcaccgttgt ccgttaatga tgttgagcag ccagtgacag ataatttgac
     2041 cagtcagaca aacttgatca atacattgtc tggtttaaat gaacagtatt ttacattcta
     2101 tacacctact ttgaagaatc agttgaatac gacgatcaca aagacaacga ctggcgtcac
     2161 ttactcagaa acagcagata accagccaaa agagctcact tgggaagtcc atgtgcctgc
     2221 aaatacacaa gcttacttaa gtttgttccc aaccaatttt gcacaattag aaagttctag
     2281 tgcgacgatc actgtcaatg gtacatcgcg aaaaacgcaa atcaacattt ctggtcaata
     2341 ctatgatctt ggctattatc ctcaagagac aacagtgaca tttactgcaa gtttttatgg
     2401 aacgaaagaa gtaagtttca tggagcctaa agtcgttggt ctagatgttg tagcctatca
     2461 agccgcaatg aatcagatca aagaaaaagg tgtcgaaatg gaagccactg gcagaaaagc
     2521 ttctgggact gtaaataccg ctgaagattc catgttggtg acaactattc cttatgacaa
     2581 aggatggaaa gctaaaattg atgggaaacc agtcaaggtc agcaatttca aagacgcatt
     2641 cttgatgata aaagttccag caggtaaaca tactgtcact ttcagttact tgcctgaagg
     2701 atttacgata ggtgcaatcc tttttgtcct atgtattgca ttgtttattc tttatgtctg
     2761 gtaccttgac cgcaatcgcc ccttttatgg cacgattgat ccagcaatag aagaaaaacc
     2821 gcgaaataca cacagaagaa gacgaagaaa ataaagaaac gatggaatct gtgtcactct
     2881 tctatttaaa tggcaaaaat ttcaatcttt aaatgacaaa aaccacctga tttttggtgg
     2941 ttttttatat acctatgact aaactatctc ttgctgcttc gacaacttct acagtgatct
     3001 tatccatttg attgattctt aaaatccgat caatctgcgc aaataaacga tgaataagcc
     3061 taaaatttcc ttttgtgatc ttaatgatag ttgtgatagc ctcataatca gaaaaatctt
     3121 ctagcttgat atcaattcct aaatctgccc atttgtattc caaaatatga tgtgtttcgt
     3181 ccttactaag gttatcaaat tcatgtgcaa atcctatgcg agagtaaagc tgcggataac
     3241 gagacaatct tttttcaata ccaggcatac cgataagaac cattgccaga tcatttctat
     3301 cgtaaatatc tcttagctgt tctaaatgct gaagctttaa acgatcaatt tcatcgacaa
     3361 taattaagtc aattgcttca taggttttag gcacaggagg atcttcttga ttgtatttta
     3421 gtaccatatg cattgcttta gtcagaccta ttctactagt tattgtgtta atatccgaac
     3481 tgacacgaga catcttttca gctggtacag tataaaaaac tttatttaca cttaatattt
     3541 catctgttgc atttatcaag aacaagaaaa agtctgaata ttcaaaaaaa ataacggtgt
     3601 acatttttaa aaggagatgt ataatcgaga tttagaaagc gttttcctca aaatttaatg
     3661 aattaggaag tgattactat gaaaaaatta tttagcgcac tttttattct gttatttggc
     3721 tttattggta ttgggacttc tggaaatata gttaaagcta gcacgatggt tgaacaaaca
     3781 agtgaaatgg caaaaacttt tagtcaatta acaccagaag aacaaagtta cttcaaatcc
     3841 aaaggattta gtgataaaga tactttttac actcaaaaga tagagcctca agatccgaaa
     3901 acacgttttt ctgcaaatgt attgcggtta accgcatcta ctaaaaaaat tactaatacg
     3961 caggggcgga cagaatatat tataacaagt cccaattcgg cgatgtatcg tatttctaca
     4021 cgcttgagtt taggtagtgc aaaatcatat actcaaacag taaatctagc taattcttat
     4081 ggttatggtg gaggaatgta ttttacattt actgggaaaa gacaatatta cagtgtaaaa
     4141 ttagctatgc aagtttataa tacatttggt gtagggtcag tctcttgcac tgcgggagga
     4201 ttgacaatag gaaaatgata atttcttact atgtcttaaa tagaagtgat gatgaaggac
     4261 tcttcgtttt tcaatcatta catgaggata cgatgctaaa agagttttat aatcggatta
     4321 agaataaaca gatagaaaca aatttatatt ttttaagtga ctctatcttg ccttttatta
     4381 gcaaccctaa aattcttaac ttgttgcaga caaaagattt gaaaagttta ccgattattg
     4441 tcattgatga ggaaattgta aaatatggcg cttatctaag cgtagaagaa ttagaaaaat
     4501 taactaacat tggaatgagt attcaggagg ataatggggt acatggaaca tagaatgaat
     4561 atgcacagtt gagaacggaa tttgac
//
LOCUS       DACCYH010000144         4575 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.152, whole genome shotgun sequence.
ACCESSION   DACCYH010000144 DACCYH010000000
VERSION     DACCYH010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.152"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            116..2056
                     /locus_tag="IVY85_002808"
     CDS             116..2056
                     /locus_tag="IVY85_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8045760.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            2143..4065
                     /locus_tag="IVY85_002809"
     CDS             2143..4065
                     /locus_tag="IVY85_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP8045761.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(4345..>4575)
                     /locus_tag="IVY85_002810"
     CDS             complement(4345..>4575)
                     /locus_tag="IVY85_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP8045762.1"
                     /translation="LIDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQA
                     VGSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         1514 a          776 c          939 g         1346 t
ORIGIN      
        1 tttttttagc gaaatttcta tttattccaa gaatttcagt tattacggaa tcattttgtg
       61 cttaataaaa agaaaccgtt tttatatact ataaatgtag aaaggcggta ggaaaatgaa
      121 aaaaattatt gcaatcacat cctgtccagt tggtattgcc catacataca tggcagcaga
      181 aaacttagag aaagcgggga aagcagttgg cgccgaggta aaagtcgaaa cacatggttc
      241 catcggtatt gaaaatgaat tgactgcaag agatatcgaa gaagcagctg gtgtaatcat
      301 cgcagcagat actaaaatag ataaatcacg attcggcggt aaacctttga taaccgtagg
      361 tgtgcaagaa ggaatccacc atgcagatga gttggtgaga gatattttag caggaaaagc
      421 gccagtctat aaaagtacgg aggcaattat ttcttccgaa aataaaagtg aatcagaaaa
      481 tttaggtaaa aaaatctata agagtttgat gaatggcgtt tcgtacatgg tacctttcgt
      541 agttacagga gggttattga tcgcgatttc tttaacatta ggaggaacag ctacacctga
      601 agggatcaag ataccagaag gaacgatttg ggcaacgatg aatagtatcg gaagtatcgc
      661 tatggggttg atggttccaa ttctatctgc atttattgcg caaagtattg cagatcgtcc
      721 aggacttgtt cctggatttg ttggtgggat gttagcggca aacggagcat tatatggtag
      781 tgatgcgaat gcgggattct taggcgggat catcactgga tttttagcag gattcattgc
      841 tttaggaatc aagaaagtgc gagtaccgaa agctttacaa tcgatcatgc caattattgt
      901 tatcccaatt ttaggatcat tggcagttgg ttttgtgttt atctatgtta ttggagaacc
      961 agtagctctt ctatttagtt cattgactca ctttttagca gggatgcaag gaggaagtga
     1021 aattgttcta gctatgatct taggagcgat gattgctttt gatatggggg ggccgttcaa
     1081 taaagtagcc tttttgttcg gatctggctt gattgcagaa ggcaattatt ccattatggg
     1141 gccaattgct gtagctatct gtatccctcc attagcattg gggctggcat cattgatttt
     1201 aaaaaccaaa tttacgaaaa cagaacgaga agctggtaag gcgtccttag caatgggact
     1261 gtttgggatt acagaaggcg ctattccgtt tgcttcaaca gatccactac gggtgattcc
     1321 aagcattgta atcggggcaa tgacaggaag tgtgatcgcg atgctttcag gagtgacaga
     1381 tcatgtagct catggcggtc cgatcgtagc ggttttagga gcagtggatc acgtagccat
     1441 gttttttgta gcagtgattg ctggtattgc tgtaaccgtg ctaatgttaa gaatccttaa
     1501 accgacatta caacaaactg cggttgctgc agcaaatggt gtgacagtag cagatctatc
     1561 agagactgaa acaagtgatg tttatacgaa aactgaaact tttgattcta tcacggatat
     1621 tatgtcagat gaagcaatcg ttttaggtga aacagcaaca acaaaagacg gagtaatcga
     1681 agatttagtc caacgtttag aaaaaacagc ggcgatcaca gataaaacca gatttaaaga
     1741 agtcatctat gatagagaac atgaaagtac aacgggaatc ggtatgggga ttgctattcc
     1801 acatggaaaa tcagcttatg ttcttaaacc aacagtggca tttgccagaa gtcaatctgg
     1861 ggtagattgg cattctttag atggaaaacc tgcacatatg atctttatga ttgctgtacc
     1921 tgaaaacagc caaggtgata tgcacttgaa aatactgcaa cgtctatcac gtaaattgat
     1981 ggatgatgac ttccgtcaag cactgatgga agcgccagat aaaacaacag ttcaccaatt
     2041 attaagcgaa atgtaacata agatgaaaaa tcgaccgtga tgagacataa tagatgtgtt
     2101 atcatgatcg attttttatt tagaagggag tttctgcaca ttatgaatca gcgtcagcaa
     2161 tctttgatct atcaattaag tgagaacaat aattatataa caacaaaaaa gctagcagag
     2221 catttccatg tgtcagaaag aacgattcat agcgatttga ataccattga aacttggttg
     2281 agagacaaac atctgccttt aatgtttgaa agaaaaaaag gtacaggtat cttgttgaat
     2341 ggtctgcctc acgaaaggga acaactgaaa cgagcgctta atgaagagac tcaaacagaa
     2401 atggataaaa aacagcttcg tcagttattg attttccatc tgctagtagc aaaagacgga
     2461 tttagtcttg aagaactttc agaaaaacta tatgttggaa aacgtacgat tcgtaaagaa
     2521 ttgacagagt tgcaaagctt ttttgagtat caccatttac agctagtatc caaaacaaaa
     2581 ctaggaactt ttcttaaagg cgatgaacaa gaaaaaagac agttgcttgt taagacatta
     2641 cgaaatatgc agaaagaaga tccacaaagt ccgtcgttga aagaattctt cgctaaagat
     2701 acattaaaag ttatccaaca tacattacaa gatgtttttt atgaaaacca tttagaacag
     2761 ccgaatggac tggctagtgt ggagattcac atctacttta tgctcgagcg aatgaaacag
     2821 taccagaaag tgaaattaag taaagacgaa aacgaagtcg tggaacacac ccaagcacaa
     2881 aagttaagta gtcaaatatt agcaaaacta gccaccatct atccaattga attttctcct
     2941 gatgaaatca attatctggc tttgcgtatc gccaatcttt ttacaaattc acaagcagcc
     3001 acacgttttc aaaaggaatc tagcgcactt gctgatcatt tgatcgaaca agtggaacag
     3061 tttctaggct attcattaaa agaagatcag ctactaaaac aaaatttaag gtctcattta
     3121 tcttcaactt attttcgact ccattatggt ttgcagattt ctaatccatt gacgaaaaat
     3181 gtgtttagta cgtacacaca acttttctta gttttacagt taattttaga agattatttt
     3241 gaaaaggaaa atttttatgt tccacaagag gagacagctt atttaacgat ccatttccaa
     3301 gcagcgatcg agcgacacaa acatcagaaa agtagaagat atcaaacggt tttagttagt
     3361 gaatattcaa aagctatggc tacttttctc gaagcacggc tgaacagaga acttccagaa
     3421 ttaacgatta tggatctagt tgaatataag ccagaattaa agagacaaga ttttccctct
     3481 gttgatttta tcttggctac gttaccattt aaacatataa gcattccagt aatcgagatc
     3541 tcaccaatga tcacagaaac tgatctagcc tatttgacaa aatatatgct ggagcatgtg
     3601 cctatcaaga agaaaaaaac atttgatcta gcaagcttta ctcatccatt tctgatcttt
     3661 cctcaacttg aacggacaga cccagccgat attttaaact ttatgggaaa cgtcttagtg
     3721 gaacatcatt atgtggagcc ggaattcatc gattctgtct tggaaagaga cagtcactcc
     3781 agtactagag tagcaccatt tgtaacaatc cctcacggta atccgctgta tgtgaagcat
     3841 tcgatgattt ctattgctac aatgaaagaa ccagtcctat ggcacgggga acagatacgt
     3901 atcgtactgc tattagcaat aaagaaaaat gatttgaaac atgcagagtt caaaaaaata
     3961 ttttctgtta ttcactatct agaaaagcag ccagaacaaa tggctcagtt gatgcaatcc
     4021 aatcatccgc tagaaatctt aacgttgtta tctagttatg aataagtagc gattaaaaaa
     4081 ctgcctttat atttataata attctatcta tttatttgga agtagaaaac taaatacaaa
     4141 aaaagaggat gtggccaaag ttgtggcaca tccttttatt ttgagtttct tataattttt
     4201 tatagttgct tacgatgtct tgactactgg taaacggtgt tcaagagaca atttgtcggc
     4261 aataaggcac aattttgtaa aatatggaaa gctatttttt taacattgtt tcttagtgct
     4321 tgaaaaatca ttttgaatac ttctttatac gatcgatgta cgttgattta agtctaaaac
     4381 ttttcttttg attccttctc tgacttttct gaattccgtt aatatttctt cttcgctccc
     4441 gacagcttgt gcaggatctt ttaggtccca gtgaaggtgt tcgtgattgt ttggaacggc
     4501 agggcacttg tctttggcat caccacaaag tgtaatgatc aagtctgcct gagtaaagta
     4561 atctctatca atcaa
//
LOCUS       DACCYH010000145         4430 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.154, whole genome shotgun sequence.
ACCESSION   DACCYH010000145 DACCYH010000000
VERSION     DACCYH010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.154"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(213..443)
                     /locus_tag="IVY85_002811"
     CDS             complement(213..443)
                     /locus_tag="IVY85_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP8045763.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(673..1491)
                     /locus_tag="IVY85_002812"
     CDS             complement(673..1491)
                     /locus_tag="IVY85_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8045764.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1652..2341
                     /locus_tag="IVY85_002813"
     CDS             1652..2341
                     /locus_tag="IVY85_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8045765.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2355..3857
                     /locus_tag="IVY85_002814"
     CDS             2355..3857
                     /locus_tag="IVY85_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8045766.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            3870..4346
                     /locus_tag="IVY85_002815"
     CDS             3870..4346
                     /locus_tag="IVY85_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8045767.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
BASE COUNT         1483 a          637 c          785 g         1525 t
ORIGIN      
        1 tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat gaactaataa
       61 ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc ataaactttt
      121 aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca taacattgaa
      181 gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt cttataaaca
      241 agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt tatagaaaac
      301 attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc agtaggttcc
      361 tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt atttaagaga
      421 ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta cacgtttttc
      481 tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc aagaaccgta
      541 ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa taagctatca
      601 agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta ggtttagcta
      661 agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt tttctcttaa
      721 ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat cacaaatata
      781 aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa tggctgtttg
      841 taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta tagcaataat
      901 ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg ttttcccaga
      961 taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt gggcttgaaa
     1021 atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat tttctaaatc
     1081 aagactagta tttccactag atcccactcc aaatatatat atgttttttg attgagtgat
     1141 cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg aatagagagc
     1201 ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat gttctttttt
     1261 ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag aaaaaccaat
     1321 tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct taatatcact
     1381 catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga cttttttttc
     1441 agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca tacaaactcc
     1501 tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact cctaaatact
     1561 ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag ttgttatcca
     1621 aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt tagatcttat
     1681 ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt acaaagagga
     1741 agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag ttggtatacg
     1801 tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac caatgattgg
     1861 aattattaaa agagattatc caccagaatc tccattcatt actgccacta tgagagaagt
     1921 agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc ttagaaatcg
     1981 acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat atcctcatca
     2041 gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata aagcaggaat
     2101 agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac aagatgggcc
     2161 agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg ctgagggaag
     2221 gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag gtattgttgt
     2281 gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag cattaagtta
     2341 gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc atgttaccaa
     2401 ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta acaaatgaga
     2461 gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt ttaagtatta
     2521 tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca attggtattg
     2581 ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga gtagcttatc
     2641 tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa gatgcaacac
     2701 tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt ttacacaatc
     2761 gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca agatttattc
     2821 cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta atttggccac
     2881 caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt agtttaggaa
     2941 cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac cacaccattt
     3001 accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga tcaacaattg
     3061 ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact ggtctgttta
     3121 cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga ttaccagcag
     3181 catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat ggtgggcttt
     3241 attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt gaatatatgt
     3301 ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga ttatcttttt
     3361 attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt ttgatcgact
     3421 ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa ttaattcctt
     3481 ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc aaaaaattta
     3541 aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa gtaataaaca
     3601 aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt ggaaaagaaa
     3661 atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg aatggggaga
     3721 ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa gtgaagggtg
     3781 gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc aatcaaatat
     3841 taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag aaaaaacaag
     3901 aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta aatgatcctg
     3961 tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt tcaaaaattt
     4021 atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct attaatataa
     4081 aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt gaattacaag
     4141 gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa acctcgttgg
     4201 ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt atagttgttt
     4261 ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt gctcatggga
     4321 attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa aataagctaa
     4381 ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
//
LOCUS       DACCYH010000146         4049 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.155, whole genome shotgun sequence.
ACCESSION   DACCYH010000146 DACCYH010000000
VERSION     DACCYH010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.155"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(160..1665)
                     /locus_tag="IVY85_002816"
     CDS             complement(160..1665)
                     /locus_tag="IVY85_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8045768.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDPFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1662..2348)
                     /locus_tag="IVY85_002817"
     CDS             complement(1662..2348)
                     /locus_tag="IVY85_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8045769.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2541..3962)
                     /gene="gndA"
                     /locus_tag="IVY85_002818"
     CDS             complement(2541..3962)
                     /gene="gndA"
                     /locus_tag="IVY85_002818"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP8045770.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1114 a          857 c          693 g         1385 t
ORIGIN      
        1 tttcgttcat ctaaaagata cttacattta attaacggat gttattctat ttaattgttc
       61 gctagttttt ttataatgac atccctgaat ttctcacttg cttgtatttt gtaccttatt
      121 ctttgttgga tatataaaaa aacaagtaaa gcacatgctt tacttgtctt ctgcctttgc
      181 gataggaata aagattctaa aaattgtccc ttgccctact acactttctg ctaagatacg
      241 tcctttatag ttttcaacca gttgtttagc gatagacaac cccagaccat ttccaccctt
      301 cgttctagct cttgctttat ccactcgata aaaacgattg aagatcttat taaggtcttc
      361 tttgggtatt ccttcgccaa agtcctgaat cgcgatctca aattcattca gagtggaaga
      421 aatagagata tggacttctt ttcgtgtagt tgaatacttt actgcgttat ccaatatgat
      481 aatgattaat tgttcaaagt gattacgata aatttgcagg gtcacttctt ttgtcaaatc
      541 atcatctaaa gtgatcacat agtctggata taatatcttg aaattattat aaacttgata
      601 ggtgacttct tttcctttag ttgtttcatt cgcataatga acatctactt gttctgctct
      661 tgataagtct agcatctctt gaactaagct tttcatacgg ctgatttctt gcatgctggc
      721 gcttaatgat tcttctaata tctcaggatc atctttcccc caacgattta gcagactaag
      781 atgcccttca ataattgcaa caggtgtcct taactcatga gatacgtctt ccacgaattg
      841 ctcttgttgt tcaatataca aacgcatacg gtcaagcatc tcattgaata tttcagctaa
      901 atctgccaat tcatcatttg tgtcgatttc aggcatgtgg atatctgatt gcggatcttt
      961 acgtatagta tccattgtat ctctgagtac cttgagtggt tttaagaaat aagaagataa
     1021 aaagaaacct aaaatactac ttaagatcaa agaaacaact tctaaaacaa taagagtcaa
     1081 taatagctta tttcgaatat catagaaaga agataattca tagaagcttt gcgcatagcc
     1141 gatcttttct cttgtacctt tagaatatat aggctggata gataaaaagc cagtttttcc
     1201 ttctagtgta acgatcgttg gaactttacg tgtagtctga atcaggtttc gactgttttc
     1261 atgggtttta aaaatcaagt tctcgtccaa atcataaaca taaagatcca ttgatggctg
     1321 ccctagctca gctataaagg gatcgatctt cattaagttt ccttcgacca gtgaagtatg
     1381 atctacgacc tcatctccgt catttgtcgc tttcgtcaga ttacgatacg tatttactaa
     1441 tgtcagttct tcatccgagt tagccaaacg agaagctact tctgaaatcg tacgctctac
     1501 attgtttctt tcttttgcca caatcagatt gatagatgat ttataggtaa tgacagcgaa
     1561 aattgtaaac acaacgaata tgaagaaaga actcgcaaag gcccacttga ttgtcaagga
     1621 gggccctttc agttctcttt tttccgattt tttcggcttt atcacgagcg catcacataa
     1681 cctgttccgc gtacagtttg gatatagctt tcctccccgg gaacgtcaat tttgttgcga
     1741 agataacgaa tatatacgtc cactacgttt gtttcaactt ctgtttcgta tccccacact
     1801 ttatttagta aaacgtcacg tgctaatacc acgttcacgt tttccattaa agtaagcagc
     1861 aactcgtatt ctctttttgt tagttcaatt acttcatttc cgcgacgtac gacacggttt
     1921 tctttttcga tagtcaaatc gcgataagta atagtagttt gtttagccac atttttatcg
     1981 ccttcaatat cgattcgacg caacaatgca cgaagacgtg ctaataattc ttcgatagca
     2041 aatggtttga cgatgtagtc atctgctccg tggtcaagtc cagatacacg atcgatcaca
     2101 gaatctcttg cagtcatcat aatgattggt gtatttttga cttggcgtac acgtctgcag
     2161 acttctaaac cgttcaattc tgggagcatc agatccaaca atatggcatc ccactcatta
     2221 tttaatgccg catctaaacc agttcggccg ttgtagtgga cttctgcgtt atacccttca
     2281 tgcttcaact ctagctcgac aaatcgtgcc aagttctttt catcttcaat gattagaata
     2341 ttgctcattt attattttat cctttctatt ctactggctt tttaaaaact ctatcattat
     2401 accgataata tgcttaaaaa tctatagtca ttttatcatg gatagcaatt ttaaaaaaga
     2461 gctgaagtct actcataaca atcgacttca gctctttcgt cctcttttta ttttctcatt
     2521 tcctaacagc cgaaagacta ttattcttca tggtaccatg agtaatggaa gattccttct
     2581 ttgtcgacac gctcataggt atgtgcgcca aagtaatcgc gttgggcttg gattatattt
     2641 gcaggcagac gttctgctct gtatgaatcg aagtaagcga tggctgatga gaaagtcggt
     2701 acaggtacgc cagcttggac agccaaggca acgacgtctc tgactgattg ctgatatttt
     2761 ttcgtgattt caacgaagta atcatctagc aataaatttt cgatctcagg atttttgtca
     2821 taggcatccg taattttttg caagaatcga gctcggatga tacatccagc gcgccagatt
     2881 tttgcgattt caccaaatgg caagtcccaa tcaaattctt ttgaagcgac acgtaattga
     2941 gcaaatcctt gggcataact catgatttta ctgaaataca acgcttctcg gattttttcg
     3001 accaattctt ttttgtctcc cgtgaactca aaatcgttcg tacgagacaa aatcttgctt
     3061 gcttgtacgc gttcttcttt ataagcagaa atatagcggg caaatacaga ttcagtgatc
     3121 aatggaagtg gcacacctaa gtcaagcgcg ctttggcttg tccatttacc tgttccttta
     3181 ttcccagctg catcaaggat cacatctact actggtttac ctgttccttc atcgtcttta
     3241 cgtgtcagaa tgtcagcagt gatttctatc aaatagctgt ctaattcgcc ttgattccat
     3301 tctttaaaga tatcagccat ttcttctact gaaaggttta aaatattctt cattaagtca
     3361 taagattcag caatcagttg catatctcca tactcaatac cgttgtgtac cattttcaca
     3421 taatgtccgg ctccatttgg gccaatatat gtgacacatg gtgctccgtc ttctgctttt
     3481 gcagaaattt gctctaagat tggtgcaact agcgcatagg cttctttttg tccaccaggc
     3541 atgattgaag gtccttttaa agctccttct tcaccaccgg aaacgcctgt accgataaag
     3601 ttgatgccag agttggctaa ctcttcatta cgacgcatcg tatctttaaa gaatgtattt
     3661 cctccgtcaa tcaaaatatc gcctttatca aggtgaggaa gtaactcttg gattgttgca
     3721 tctgttgccg gtcctgcttt gaccattagc aagatccgac gtggtttctc gatagattct
     3781 acaaattctt cgattgtata tgttgctttg aaatttttat ctgggtgttc ttctactaca
     3841 gcggttgttt tagcccctgt acgattgaat aaagccactg aatagccgcg gctttctatg
     3901 ttcaaggcta gatttttacc cataacggcc ataccgacaa cgccgatttg ttgttttgac
     3961 atatgaactc ctcctacata cgggatttct ccctaatctc ttacattata gcttaacacg
     4021 gctacattgc aaactaaaga acaacatat
//
LOCUS       DACCYH010000147         4029 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.156, whole genome shotgun sequence.
ACCESSION   DACCYH010000147 DACCYH010000000
VERSION     DACCYH010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.156"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            25..522
                     /locus_tag="IVY85_002819"
     CDS             25..522
                     /locus_tag="IVY85_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045771.1"
                     /translation="MSYLIVCLIIIICLHHLFLSKTIPRYSKNKKAEKFCLLLNKFTL
                     IAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLC
                     LVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYF
                     YRNKL"
     gene            complement(648..1325)
                     /gene="nth"
                     /locus_tag="IVY85_002820"
     CDS             complement(648..1325)
                     /gene="nth"
                     /locus_tag="IVY85_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP8045772.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1332..2030)
                     /locus_tag="IVY85_002821"
     CDS             complement(1332..2030)
                     /locus_tag="IVY85_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP8045773.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2298..3482
                     /locus_tag="IVY85_002822"
     CDS             2298..3482
                     /locus_tag="IVY85_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP8045774.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3488..3931)
                     /locus_tag="IVY85_002823"
     CDS             complement(3488..3931)
                     /locus_tag="IVY85_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8045775.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT         1059 a          785 c          655 g         1530 t
ORIGIN      
        1 aattttttga aagaaggctt cattttgagt tatctgatag tatgcttaat tatcataatt
       61 tgtctgcacc atcttttttt atcgaaaaca atacctcgtt attccaagaa taaaaaagct
      121 gaaaaatttt gtttattatt aaataaattt actttaattg ctcctatcct tgcatttatt
      181 attttttctg tacttttaag cactactctt aaaggaaaat ttatggaaag aagctctcat
      241 gcaatgattc tcactttttt atggttgttg ttcaccagaa tctatatttt tctgatgagc
      301 ctgaaaccgc ccaaatccat tagtttatgt ttagttataa atggtatttt tttattatca
      361 cttatcattt ttattacacc tttagataga tacgtcactt atttatataa tcctttggaa
      421 tactggactt attttatagg tatattggaa ggcattattt tctacatcgg gtattttccc
      481 aataaaaata acaactttta tttttatcgc aataaattgt aataagttac catcctagct
      541 actgagtatg tacatttact ttttcatgca aaaaagataa acgaaacagc ttttctccat
      601 aataacaaga taattcatac atttatttgc aattaaagaa accacgtcta caggcgtggt
      661 ttctttttta gcgccttttc ttttagacgc atccgatttt tcccatcttg acatattgat
      721 agcaaaggac atacctcgca cttgggattt cttgccgtgc aatgatatct accaaaaaaa
      781 atcagtgtat gatgtgtctt tacccataat tcttgtggta ctttccgcat cagtgtttct
      841 tctacttcca tgaccgtagc atctagtttg cagatcctca ggcgttttga gacacgttcc
      901 acgtgggtat ctactgcgat tgctggtatc ccgaatgcat cccccaatac gacatttgct
      961 gtctttctgc ctacaccagg aagagacatc aattcttctc ttgacgttgg cacttgccca
     1021 tcgaaacgtt caatcagctg ctgtgcacac gctttgatgt tttttgcttt atttcgatac
     1081 aaaccaatcg tttttatttt aagaatgatt tcatcgattg aagcttctgc taacgcatcg
     1141 ggtgtcggaa aagaggcaaa caaatctggc gttgctttat tcactgatac atctgtcgcc
     1201 tgggcactta agatcactgc aatcaataac tcaaatggat tattgtgctt gagttctcca
     1261 tgtgcttcgg gaaacattcc atacatcgtt tctagagctt ccattgtttt ttgtttgctt
     1321 aacatattcc gctactcctc caaccaattt ttcagtgaga ctttcggcaa ttcttcttgt
     1381 tgagctgact gttctacttc tctttgaaga agctgctgtt ttcttcgttt ttggtcttct
     1441 tccacttgtt gttttgtacg tagatttttt ctttcccacg atagcaaaat gcggtcaacg
     1501 taattaaaac tataagcctg atttaatacg gcttcccgca gtgctagctt caaaatttcc
     1561 ggctgataat ggtcttcttc tagccattgt ccgatccgct ggaactcgat cgaagaaagc
     1621 gggcgtccaa attcttgttc aaataattga tacactgact ggatttcttt ttcttgtgtt
     1681 ttttcttcgt attttttttc ttgtgttttt aaatactcac ctaatagatc atagatgggg
     1741 taaagattat atcgatctgc ttttttaccg ttgacaatcg tcgtttctat cttgataaat
     1801 ccatttgtta cgagacgatc cagtatttga aaaatctgat cttgtttgat tcccatatcc
     1861 atagcaatca tctgcaaatc agggaaagta tctccttcaa gctgtgccat atgcaactgt
     1921 agaacaaata aaaattcttc agattgtaga ccgatcctat gataattttt aagtaaaaga
     1981 ttagatatga ctgtttgtcc agcgcttaag taattttcta aattcaacat gatttctcct
     2041 ccgctcaatc acagtatacg taaaaaaaac atgggaagca agaacaggtt tcttttctat
     2101 ttacttcact gatcggtcag atttacaatg tcgtctactt gatttttgtt tgttcatgtt
     2161 tttgatgcgt atcttcactt ttcggaataa ataaattaag tagatttcgc tgttccttag
     2221 aatgttgaca aggaaacaaa attactatac actatttttg tttccaagtt aacattattg
     2281 cataaaggag tttttacatg tacagtttca caaataaaca ttctaaagtt tttggatacg
     2341 tctctgtctt attcactgga ttaggattta ctatcgtcag tcctgtactt ccgtttttaa
     2401 cgctgcctta ttctcactcc atacatcagc aagctttcta tatcactttg ctcatgtctg
     2461 tttatgctct agctgccttc ttatctgccc ccattctcgg tagcttaagt gatcattttg
     2521 ggcgtcatcc gatccttatt atcagtttat taggatcaag tatcggatat ttgctttttg
     2581 gtttaggaaa ttcgatatgg atgttgttcc ttggtcgtat cattgaagga ttgacggcag
     2641 gagaaatctc caccttatat gcttactttg ccgacatcac ggaacctaat gaaagaacca
     2701 aagtttttgg atggatgggt gctttagtcg gtataggaac aacgctgggt cctattatag
     2761 ggggtctatt agctgagttg gggaattctg ttcctatatt tattggagca ctctttactt
     2821 tcttaaatgc cgtttatggc tacacattta tgctagaaag ccttccaata aaaaaacgtt
     2881 cagtagattt aagtttctcc catgtaagac cttttcacca attacaacaa ttattcaaga
     2941 tttcatccgt tattccttta ttgacagcag gttttgctgt ttggttggca gctggctctc
     3001 ttcaatctat cttttctcaa ttttcgattg acgcctttca gtggaaggct gggttggttg
     3061 gtttaagctt ctctttgatt ggaatcttgg atactgtctc gcaattgttc attatgcctc
     3121 gattgctcaa aaaattttca gaacagcaga tcacaagaat agggatgttc agtgaaatcc
     3181 tcgcctatct attcatcaca ttatctggaa ttcttctact gccgattttg tttttatttg
     3241 ggattatctg ttatggtttc ggtgattcta tttttactcc tgtattcaat ggccaactat
     3301 ccaattctgt ctcagataac cagcaaggtc ttgtaatggg cggtacccaa agtatacaat
     3361 ctctatcgcg cgtcatcggc ccgctaatcg caggacagct ttatgctgca gcaccatttc
     3421 ttccgacggt attcggattt ttgcttatga tttttgccta ttatcactac aataaaaaat
     3481 agtagcttca tttgctactt cgtataaatt ctctgtatcg accgataaaa cgaaatactg
     3541 tgtcaaattc ttcttttgac atattttcaa atacagtagc atctcgctgt acccattttt
     3601 gatggagttg ctcgtgtcta ttccttattt tttgtccgat tggcgtcaat tgaaaataga
     3661 tttctttttt attctcttcg ctttgataag tgtttatgat ccctctagct atcagttttt
     3721 ttgttagctt actgatggct cctctagtca tgtaactagc attcgctaat tttgtcacgt
     3781 taggatcagg gattttcccg atatgttcaa tcacttctag ttcatttaga gtaaatcctg
     3841 caaattcttt ttccatttct ggtttattac gatgaataat ctcattagct aaatccagat
     3901 attcttctat tagcagttgt cttttatcca tttctacacc ttctttttct ttcattatat
     3961 tgtttttttg tttcctattc aacaaagata aaaatatttt gtaggaaaac ccaatctgaa
     4021 accaagaaa
//
LOCUS       DACCYH010000148         4022 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.157, whole genome shotgun sequence.
ACCESSION   DACCYH010000148 DACCYH010000000
VERSION     DACCYH010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.157"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            86..1435
                     /locus_tag="IVY85_002824"
     CDS             86..1435
                     /locus_tag="IVY85_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8045776.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1563..2153)
                     /locus_tag="IVY85_002825"
     CDS             complement(1563..2153)
                     /locus_tag="IVY85_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8045777.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2300..3652
                     /gene="brnQ"
                     /locus_tag="IVY85_002826"
     CDS             2300..3652
                     /gene="brnQ"
                     /locus_tag="IVY85_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8045778.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1152 a          741 c          784 g         1345 t
ORIGIN      
        1 aaaattttgg aatcttagtt gtgtttttca gaaaactctc ttaatatgga agtaattaaa
       61 cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc gtggactgaa
      121 aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt tgtttttagg
      181 ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc ttattactgg
      241 atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa acaccaataa
      301 ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt tcatcactgg
      361 ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag ccatcggaat
      421 gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga ttgcattagg
      481 tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag agttttggtt
      541 tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt atatgatcct
      601 cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt ctcatggcgg
      661 attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa cctttgcttt
      721 tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg aaaaagtgtt
      781 gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag gatctctttt
      841 tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc catttgtaga
      901 agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag tcgtattaag
      961 tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc ttcgttcttt
     1021 agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc atgtgccagg
     1081 gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga attacgtgat
     1141 gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct tgtttatctg
     1201 gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca aaaaagctca
     1261 ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt acatggcctt
     1321 tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac taacacctgt
     1381 gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga agtaagaaag
     1441 tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa aattgcttct
     1501 taaatttttg tgaaatttcg gcttatttct tggagataaa agcttctgtc ccagcctctt
     1561 ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata ggaaaaaatc
     1621 caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa aaagacaccg
     1681 gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt tgacaaaact
     1741 ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa gataccaaaa
     1801 gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa agcttcaggg
     1861 ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag caaaaagata
     1921 ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg atgatggttc
     1981 acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt atttcggatg
     2041 acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac aagaactctc
     2101 aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt cacgatgatt
     2161 cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata accatcgttt
     2221 tatataggga attttctgaa aattgttgca tatttttcga aattttgtta cttttaaaaa
     2281 aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc tttttatagg
     2341 ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg ttcatttggg
     2401 tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta ccggtatcgg
     2461 tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg tatatgaatt
     2521 agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt acttagttat
     2581 cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg gtttagcacc
     2641 atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg tattttttgt
     2701 gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg gaaagttcct
     2761 gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt tgaatccact
     2821 aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt ttttcacagg
     2881 gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg ggatcatcat
     2941 tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta aagacacgat
     3001 caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc tcgcttattt
     3061 aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca cgctggctca
     3121 gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta tcgttatatt
     3181 agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat tcacagattt
     3241 gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc cttgtttagc
     3301 ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga tgttcatcta
     3361 tccattagca atgacactga ttttactagt gttagttggt cctttgttta agcagcgtac
     3421 ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg atgggttgaa
     3481 cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat atgttgcgga
     3541 aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag ttatcggttt
     3601 tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt gataaaagag
     3661 gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa ttttgaaaaa tggcttctca
     3721 catttttgca taaatttgac ttaataccga gaaactgact tttgaacacc gtttattgag
     3781 aggtcgtgaa aaagccgctt agtttcaagt attaagaaat attttgtgaa aatggcttct
     3841 cacatttttg cataaatttg acttaatacc gagaaactgg cttttgaaca ccgtttattg
     3901 agcgtctata aactgacctg agtattagag aaacaaaacg gcaaagtagc tccatatact
     3961 ttgccgtttt gttttgaact aataccgaag agaagctgat caaacaacat ttcttttata
     4021 at
//
LOCUS       DACCYH010000149         3876 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.158, whole genome shotgun sequence.
ACCESSION   DACCYH010000149 DACCYH010000000
VERSION     DACCYH010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.158"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(716..3865)
                     /locus_tag="IVY85_002827"
     CDS             complement(716..3865)
                     /locus_tag="IVY85_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP8045779.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          803 c          635 g         1362 t
ORIGIN      
        1 actaatacag gtagtcctct tttttgttat tttgatattc taaatcattt tttattggat
       61 tattcccttg ttcgatatag acccaaattg atctatcaat aattatcaaa ctgagtcata
      121 tagagacaaa aatttcaaaa atgacctgtt aaatggtcat cgaatatcat ttaaaatcaa
      181 tacgacggag tcagacacgg agtcaaaaca agcccgctga gagtaaaaaa tatatacagt
      241 cccataggta aagtgccaca ggactgtata tttttttatt tgagattacc tgaaatgatt
      301 ccatttactt tttatctcat tttttcctac tagttagtat caaatactct cctattttct
      361 caataaacta ttttgggtta catggtatct gaaacagcta aaaccttgtc attacagcaa
      421 aaaatcactt tatctccgtt tcatttcaga tacatttcac ttaagcggat tccaaaaaaa
      481 caaaagaaaa aggaacccct agttagccta tgctttttat tggttcctct caaaacacgg
      541 tcatataaca acggggtggg gtaaatgaat gaaaaaggtg gaggaaacat tattcaatag
      601 ctgtcttaaa tacttcgtta taaaatgtga acaaataaaa gacatctatc cctattttct
      661 acacttccac tttactacca tctttgaatc gtacaatttt cttaactctt ctcgtttacc
      721 tcaattcatt ttcaagtgga atcagctcat catctaacat tttacgccat gctcgcttca
      781 tttcttgtgg gtatttaaat ggcttcgctt ctggatggtt ggctttatat ccttcgtaat
      841 ccttactgtt gataattgct ttcatattag gaattggatc ttcacctggc atatattgca
      901 gattagaagc atgtaattct ttctcagaaa caaaccactc attggaaaag acatgaatca
      961 aatcatcact cgcttgtgta aaatagcgac gtttaatgac aaagatatct tcattactat
     1021 ctaaagtccc cacatcaaac gcatttaaaa tttgtcggta aacttctttg actccttctg
     1081 ttttattcaa tttagccaaa gctttttcaa tttcttctcg tgcatctgaa ctattcgccg
     1141 cataagttcc tagtaattga tcaatataag tagaatcaat atcatacaac ttcgtggtat
     1201 tttcactata aaattcgatc gtggaaaaat cttctccctc tttttctggt tcttcctcta
     1261 ttaatgaacc cttcacggtc tcgtacacgc caatttgttc ttccaaaagt tgaatttgat
     1321 tgttcaaggt tgaagatttc accatgtcat cattgtaatc gtcataagtc actaacgatt
     1381 cataagcacg attcaattct tggaatgctt tgacgtactc gatacggatt tcaagtggcg
     1441 tatgttcatc aatggattcc atggatgaaa cctcttttaa cttaccatat gcttgcttga
     1501 atcttttttt ggattcctga taacttggat aaatcaaacc cgattcttct tttgcttctg
     1561 aatacagttt tgtcgcatcc tcgacattct tctccattgt ggctggcttt cggaaagtaa
     1621 caattagtcc ttttgttttt tctggatacg tacggttcgt acgtgaaaaa gcttggatca
     1681 atcctgcata ctctagatta cgatccacaa atagggtttg aatcgtaggt gcgtcaaaac
     1741 ctgtcaatag acgatccact acgatcacta aatcaatttg tttcccaaat tgtttgaatt
     1801 ccgctctttt tcgtgccaaa cgattattga tgtctccgtt ataacgctca atgtctgcta
     1861 atgaccagga agtatcataa tactgattat attcctcaat aattttttgc atttcttctt
     1921 gttggacact tgaatctcgg ctgttttctt ctaatgaata ggtaatagcg attcgtggaa
     1981 aatcaggatc ctccatcgtt cttccttcac gaatgggctg attaggaaac tcttttgtta
     2041 accagtcagg ttctttcgtc atttctttga tcgctcgata atactttttg gccatgtcaa
     2101 tggaactcgt ggttaagatc gcagacttcg ttggacggcc atttcgaaaa tcaaattttg
     2161 tataggcatt gtctggacgg aacattttat gaatgacttt ttggatatgt tcatctgctt
     2221 caaaaacagt agggtccaaa taactttctt ttttgacagg ctccatttga tcaatcatac
     2281 gattgatctg ttccggtgaa tactctgcat atgtttctac ttcttttaat ttccgataga
     2341 ttttattctc taatgaagtc ggctccacgg tgttttcatg ctctacttga aatcctaaaa
     2401 ccgagccatc ttctaatgca ttcttgatgg tataggtatg caagacctct ccatactgat
     2461 catgggtcgt acgtgccaat tgccctttgg cttgtttccg attctcaggg aaaatcggtg
     2521 tacctgtaaa gccaaaccaa gtggatttcg gaaacatttt tttgatttct tccatatttt
     2581 cagcacttaa cgctcgatga cactcatcta ctacaaacac gatatgttgc cccattaatt
     2641 tttggaagcg attggttcct tttttctctt cttgtttttt tgcatatttc aacgccgcat
     2701 ctagcttttg acgggttgta ataatcacaa catttgcatt ggcatctgcc aacagagttt
     2761 cacttaactc tttggcactt cctgttccca cgatcaagct attcgctttg gcgttcccgg
     2821 aagaaatccc agtattaaat tccgaagcaa atttcgtaaa ctctgtggtg gtttgattat
     2881 ctaaatcttt gcggtccact aacatgatcg tccgatcgat ccccgatttt cgtgctaaaa
     2941 gcttagtaga tacaaaacta gtcaacgttt ttcctgaacc agtcgcatgc cacacatatc
     3001 ctgattgatg tttatttgca gcaataaaca aggcttgaat cgcatgtact tgataaggat
     3061 gtaacaccat gaggatttta ttgtcttgat cttcactgac aatcgtataa tccgcaatca
     3121 agcgatgagc atccgggata tttagcactt gtttgcaaaa ctcatataag ttttctacct
     3181 ttttattatc tttcgttcgc caactaaata aaaacttagg atgcaaatcc tttggtaagg
     3241 catttgcaaa ataccgagtg gtttgttcat tggaaaccac aaacaactgt aaggtcgaaa
     3301 agatattgtt ccgaaacata ccttcttctg catatttttt gatttggtta aacgcctgat
     3361 aaaaactatc ttttgcggtt acttgtttca gctcgatttg gacaatcggt aatccattga
     3421 tcagtaaggt gacgtcaaaa cgtcgatccc gtgcttctat gttacttcca cgttttgcaa
     3481 tttgatggac cacttcatag gtagagattc ctccaccaat gtcttgatta gaataaagaa
     3541 tgagcgagac agaacccagt tgcggatctt cccgttcaat cgtgatccga gccattccat
     3601 tttcaccttt taaccatttc gcagcatcaa agggggtttt cgttcgtagt aacaattctg
     3661 ttttgatcgt ttcaaattct ttatcggtca aaggaaaatc attcagttct gcttgattat
     3721 ttgagatgat tttttggcgt aaattgaccc aaagatcttc ttctgatttt agatcgggac
     3781 gatacgtcca ctgattatgc ccttccccca atacacggat caactgttct tcgacttccg
     3841 cttcctcacg gtgactaatt ttcatttatc ttccct
//
LOCUS       DACCYH010000150         3809 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.159, whole genome shotgun sequence.
ACCESSION   DACCYH010000150 DACCYH010000000
VERSION     DACCYH010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.159"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(113..610)
                     /locus_tag="IVY85_002828"
     CDS             complement(113..610)
                     /locus_tag="IVY85_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP8045780.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(740..1450)
                     /gene="budA"
                     /locus_tag="IVY85_002829"
     CDS             complement(740..1450)
                     /gene="budA"
                     /locus_tag="IVY85_002829"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP8045781.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1463..3127)
                     /gene="alsS"
                     /locus_tag="IVY85_002830"
     CDS             complement(1463..3127)
                     /gene="alsS"
                     /locus_tag="IVY85_002830"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP8045782.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3332..>3809)
                     /locus_tag="IVY85_002831"
     CDS             complement(3332..>3809)
                     /locus_tag="IVY85_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8045783.1"
                     /translation="CIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLFAWIV
                     SVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVLRIAF
                     YYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTMDL"
BASE COUNT         1132 a          821 c          604 g         1252 t
ORIGIN      
        1 taacacttgt gattttataa aaaaataaaa aaaccgaaaa acagaaatgc ccactgtctt
       61 tccggctctt tttattcttg atttctcttc ttttttgttt caatagacga ttttagttat
      121 taataaatga aaaagcattt atattaaaat ttgctaataa aagatctaca tatttttcta
      181 gaaatgcttg atcgatttca tcataccctt tcgttactcc gcaatcgata tctaaaacac
      241 cgattaaatt accttctttt accattggca ctacgatttc tgattgtgcg gctgaatcgc
      301 aggaaatgta atttgcatgg gttttgacat catcaacgat caatgtttct tgtttttcag
      361 ctgattctcc acaaactcct tttcccatct tgatacgtgt gcaagaaact cgcccttgga
      421 acggtcctaa aatcaattca ttaccatcga ataaataata tcctgcaaag acagtctcag
      481 gcaaagtctc attcaacaat gcagatgaat ttgctaaatt tgctaaccag ttatcttctg
      541 cttctaataa tccttgttgc tgtaataata aaatttcgta tgcatctttt ttctgttttt
      601 ctgtccacat gataaaaacc gctccttttt gatctatgga gaagattata gctcagtttt
      661 tttgtaaacg ctaggtttcc tcctcaaaaa aacgagatac gtcaggttaa tcaccttgaa
      721 cgcatctcgc tgtctttttc tattctgctt gttggatttc tgctgctaaa tcagcataat
      781 caatcgtttc gtgaacaaaa gtcggatcat caactggaaa atgctgtaca agggacgaga
      841 tgcggctaat ctcaactttt ccttcagcca cttcaaaatc cattacatgt ccgccaaatg
      901 tatgtgtatc atcgataaaa tgcaagtgaa atcccgctgc agcaactcct tggaataatt
      961 caggcgtaaa gaacccgact aatgttcctt ggacattgct ttgttggaat tctggttgag
     1021 actctgaaat ttttgctagt cttctgtaag gtttttcttg cttcggcatg atacggatgt
     1081 gcatattttt gaataatcca gttatcttca ctgccaaaaa taggttttcc ccggcttctc
     1141 ttactaatat gtcttttttc aattgttctc catctgcagt ttttgagaca gaaaaactag
     1201 tatctggcgt aaaatctgta attgctgcat agggggtaag ttcacttcca tctaattggg
     1261 caactgagcc atctgatctt ccttgataag ctacgccatc taaaaagatg acttcaccat
     1321 caagcccatg caatgttccc aatcccagag ttcctttttc taatatatga gcaatcgttt
     1381 ctgttccatc catcaagcca gccatcaaag ctcctaatgt cccgtgctgg tataagattt
     1441 tttcagtcat gtcgacactt ccttaataga attgatctgg taagattgtt tctcccaatt
     1501 tctcattatc acggtaatca atcggaatat caataattac gggtccttct gtcgcaaatc
     1561 cttcttctaa agctttttct aattcctcta cacttgtagc tcggattcct ttagcgccaa
     1621 aagcttctgc atatttcaca aaatcaactg gaccaaagtc tacacctgac gcccgctgat
     1681 atttcatttt ttcttggaac tctaccatat tgtagtgtcc atcattccaa atcaaatgaa
     1741 cgatattcaa ttttttacgt accgccgttt ctaaatcctg ggcagagaat aaaaatcccc
     1801 catcgccgga aactgatacg atttgtgtat ttggacgcac caatgcagca gaaatcgccc
     1861 atggcaatgc aacaccaagc gtttgcatac cattactgaa taacaaatga cgaggttcat
     1921 aacttctaaa gtggcgtgcc atccaaatat aatgactacc tacatctacg gtaacagtca
     1981 tatcatctgt tacttttgac tgaagcgtgt taataacttc taaaggatgc aagatacctg
     2041 cttctccttt tgattgaaca atgtcccgct ccgttaattc tgcttgaaga gatgctaaat
     2101 attcttttgc atcttctgac acttgttgag gctctaatga acctgtcaat aaatcaagag
     2161 tggctgagat atcaccgatc aattctcttt caggctgcat aaaaggatcg atttcagctg
     2221 gcgcttcatc gatcacaatg atccgcgcat ctttttccgc attccagttt cttgcttcat
     2281 attcgattgg atcatagcca atagcaataa ctagatcact tcgtttcaaa agcatatctc
     2341 ctggttggtt tctaaataaa cccacacgac caaagaaatg cgcttctaat tcacgtgaga
     2401 tcactccagc tgcttggaat gtctctacaa caggcaatgc tgtctttcca actaattgcc
     2461 tgatggctaa cgtttctttt tcacttgatc ctcgcatacc tactaatagt accggtagtt
     2521 ttgcttctct aatgcgttca gctaggtaac ggatatcatc tgcagaagca gaacctaatt
     2581 taggatcgct caatggcttg atgacattcc cttggacagg tgcatccact acatcttgcg
     2641 ggatactgat aaagctcgcg cctttttttg aactcttagc catacgataa gcattggcaa
     2701 tcacttctga aattgtttct ggatcttgga tttctgcact gtattttgtg attggctgga
     2761 acagtgctgc attatccatg ctttgatggg tcaacttcaa tagatcgctt cgttttactt
     2821 gtccggcaat tgctaaaact gggtcacctt ctgctgtcgc tgttaccaaa cctgtagcta
     2881 agttactagc accaggacca cttgttgcga taactactcc tggctctcct gtaatacgtc
     2941 cgattgcttg tgccataaat gcagcatttt gttcgtgacg agtcacgatt aattcaggac
     3001 cttgatcctc taattcatta aatacaccat cgatttttgc acctggtata ccgaatacat
     3061 aatcaacatg atgattaatc aaactttcta ctacagattt cgaaccttgt ttcatttttt
     3121 cgtccattga acatcgtcct ttttatgctt tatttttaat tattagttag aaataatcac
     3181 aaataattga tacagtttct aaacctgttt cattcaaaag tatagcacgt tttatttcat
     3241 ttgtttagcc atatgtttca aaaaatgaaa aagaatgaca gtttttacat aaaaactgtc
     3301 attcttaatt gaaagtttca atttataaag tctacagatc catagttctc atcaatttac
     3361 gattgttgaa gtaaatcaaa ccaaatacga tagaaggacc tagtagaata caccattggg
     3421 gctttacgaa aaagaagaga aacaaacttg tcaatagtaa aaaatctagt aaactcacat
     3481 accatttttt caacgtatag tagaacgcta tccgtagaac ttgatttggt ttcgacgctg
     3541 gatttctcac ctgaaaatac cagtagttga tgctaaagga aactgataaa caagcaagta
     3601 aaacaaacaa cggaatcaac cattttccat aagaaaaacg tataaaaaag aaacaatcaa
     3661 aaatcattat cacactaact atccaagcaa atagccaata aaaaaatccc tttagccaaa
     3721 attggcgaaa cgtctttatg aacaaacgta ctggtgaaaa acttttttct tttgctaatt
     3781 cttctgtcat acctatcatc acgatgcaa
//
LOCUS       DACCYH010000151         3764 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.160, whole genome shotgun sequence.
ACCESSION   DACCYH010000151 DACCYH010000000
VERSION     DACCYH010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.160"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            242..1957
                     /locus_tag="IVY85_002832"
     CDS             242..1957
                     /locus_tag="IVY85_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8045784.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            1960..3723
                     /locus_tag="IVY85_002833"
     CDS             1960..3723
                     /locus_tag="IVY85_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8045785.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT         1128 a          733 c          746 g         1157 t
ORIGIN      
        1 atgcagaatt catttataga aaaattaagt aaaatagacg ttcctatgaa tgaagagact
       61 ggcatgtttt ctcattttct tttttttaaa aaaaatagtc tattcaattt ctaaagcttc
      121 ttcataataa aactttcatt tatgaaaagg cttgttctct tttcattcta aatttcctaa
      181 gctacaatgt ggtagtactt gttttttcaa ttgtggacaa ataaataata ggaggaatca
      241 tatgattcag ttattacgtt atgcaaaaga ctatcgtaag caaatcattt tgggcccgtt
      301 cttcaaattt cttgaagctg tcttcgaact tgtcctgcct ttaatgatgg cttccttgat
      361 cgataatgga ctaaaaatga atgaccgagg aaaaatcatt gaaatgggct tatggatggt
      421 cgctatgtct gtgattggtc tgatttgtgc tatcatttgc caatactatg cttcagtagc
      481 ttcacaagga tttggtacag agttacgtaa tcagctaatc aaaaaaatca atactttctc
      541 tcacaacgag ttgaatcatt ttggaacaga taccttgatc acccgaatga caaacgacat
      601 caatcagctt cagctagctt tagcaatggt cattcgttta ttgatcagag caccgttttt
      661 aagtatcggg tcagtggtca tggcttttgt gatcgactgg gaagtaggtc tgtttttctt
      721 agcattattg cctattttct caatcatttt attttttatc atcaaaaaaa ccgttcctct
      781 ctaccaaaaa gtgcaagaaa aactagatca gctcaatgaa cacgttagtc agaatcttag
      841 cggtgttcgt gtcatccgcg catttgcgca gacaaaaaaa gaaacagaga agttcgataa
      901 aagcacaaat gatctagcga acaattattt acgtgtttcc aatatctctg ccctattatc
      961 acctgctaca accttgatca tgaatgtcgg gatcatctgt ttgctgacag ttggcgggat
     1021 caaagtaaat attggtagct tgcaacaagg acaagttctt gctttgatca attatatgaa
     1081 tcaaatgctt ttggcactga tcgttgtttc aaacctcgtc gtgatcttca cacgtgccga
     1141 agcttcagga aatcgggtga aagaggtgct agacacagaa aattcgattc ttgaagctga
     1201 ggtcagtact actcccgatt tttcttctga agcaattatc gcttttgacc atgttgactt
     1261 ccgttatact cctgattctg gtctttctct ccaaaatatt acatttcagc tgaagcgaaa
     1321 tacagtcctt ggaatcattg gtccaaccgg aagcgggaaa acgactttga cacaattgat
     1381 tccgcgtttc tatgatgtca gttccggtac agttataatg gacggaatgg atgttcgttc
     1441 ttggcagctg gatcatctcc gtcagcaaat tgctcttgtt ccccaaactt ctgtactatt
     1501 taccgggact attcgtgaaa accttcaatg gggtaagaaa gacgccacag aaaaagaatg
     1561 ttgggaagct ctgaaaatcg ctcaggcaga agaatttgtc cgtcagcttc caaatggttt
     1621 agatacaaaa gtcatggaaa atgggaaaaa tttctctggc ggacaaaaac agcgactgac
     1681 gattgcaaga gctttgattg ccaagccagc attactgatt ttagatgact ctttaagtgc
     1741 gttggattat caaacagact tggatttgcg taaggcgcta cagacttatc ttggcagtac
     1801 agtgattatt atctcccaac gtgtccgctc tattcaagaa gcacatcata tcttagtgat
     1861 ggatcaaggt aagattgctg ctcagggtac ccatgaagaa ttacttgctc aatcagcaga
     1921 atatcaggaa atcgtcgcat cacaggagga agaatagtta tgaaagtaaa aaaattatcc
     1981 ttgcagacgc tccgccgatt cattccttac cttactgcct atgcaaaaga catttggcta
     2041 gctgtgatac taggaatcat caatgggatt gcaacagttg tgatgacgtt acaaattggt
     2101 caaagtatcg atcaaatgat tggagaagga caggtccgct tcaatcgact gttccattta
     2161 cttattctat tcggtggtat cgttttaatc aacgtagtca gtcaatggct catccaagtg
     2221 ttaagcaatc ggttagctta cttatccgtt gcaaaattaa gaaaagatgc atttgctcat
     2281 ctgaatcgat tgccacttcg ttattatgat caaaactccc acggaaatat cgtcagtcga
     2341 tttacaaatg atatggacaa tatttccatt gcaatttctg cggccttcaa tcaattattt
     2401 tctggagtgg caatcatcat tctttcgttt atttttatga tgcgtctaag tccacttctg
     2461 acattggtcg ttctttgttc tacaccaatt attttccttg tgagttggat cgttgctcgt
     2521 gcttcacaaa caaatttcga tagacagcaa aggattattg gggatatctc gggctttgtg
     2581 accgaacgaa tcggtaacca gaaaatcgtg aaagcttttc agcaagaaga agctagtcaa
     2641 cagcaatttg aactgctaaa tgctgatcta tatgaaaaag gacaacgggc acaattttct
     2701 tcttccttga caaacccgtc ttctcgtttt gtagatcacc ttgcttattt atcgattggt
     2761 ttagttggcg gcctattggc attgaagagt ggttcgtcaa tcactgttgg tgtgatttcc
     2821 agctttacga tctactcaag tcagttttct aaaccattta tcgaactttc tggaatcacg
     2881 acacaactgc aaaccgcttt ttctggtcta gaacgagcat ttgatttgat cgatcagcca
     2941 gaagaaaagc ctgatttgcc agatgcctat gccttggaac ccaaaaatat caaaggaaaa
     3001 gtctcttttg atcatgtttc tttttcttat gaccccaagc agcgtttgat cgaagatttc
     3061 aattttactg cttatccagg tcaaaccatt gctatagtag gaaaaacagg ggccggaaaa
     3121 tccacacttg ttaatttgct gatgcgtttt tatgacacaa caaaaggaac aattacagtc
     3181 gatgaccatg acatccgctc tatccaacgt gatacattac gtaaaagttt tggaatggtg
     3241 ttacaagata cttggctatt tgacagtaca ttgcgagaaa atcttcgttt tgggaatccg
     3301 gaagcaagtg acgcagaaat caatgatgcc ctaaaagcct cttatatgta tgaatttgta
     3361 gaacgtttgc cggaaaaatt agatactaag attggcggtc aagggttaaa gatatcagat
     3421 ggacaacgcc aactgttgac gattgctcga acgatgatca gtaatccccc tatgttgatc
     3481 ttagatgaag ctaccagctc agtcgataca ttgacagaga aaaaaatcca acaagccttc
     3541 ttagcaatga tggaaggaaa aaccagtttt gtgattgccc atcgcctgtc cacaatcaaa
     3601 aatgcggacc agatattagt gatggatcag ggagctattg tcgaaatggg cacccatgat
     3661 gagctgcttc aaaaagatgg ttattaccga cggctctatc aagcacaatt cacagcacaa
     3721 taaaaacaag cagtcttctg ggatgggaag actgcttgtt tttt
//
LOCUS       DACCYH010000152         3719 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.161, whole genome shotgun sequence.
ACCESSION   DACCYH010000152 DACCYH010000000
VERSION     DACCYH010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.161"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..354)
                     /locus_tag="IVY85_002834"
     CDS             complement(<1..354)
                     /locus_tag="IVY85_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045786.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRTF"
     gene            complement(614..1162)
                     /locus_tag="IVY85_002835"
     CDS             complement(614..1162)
                     /locus_tag="IVY85_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045787.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1163..2020)
                     /locus_tag="IVY85_002836"
     CDS             complement(1163..2020)
                     /locus_tag="IVY85_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8045788.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2306..2592)
                     /locus_tag="IVY85_002837"
                     /pseudo
     CDS             complement(2306..2592)
                     /locus_tag="IVY85_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2582..2911)
                     /locus_tag="IVY85_002838"
     CDS             complement(2582..2911)
                     /locus_tag="IVY85_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8045789.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2988..3260)
                     /locus_tag="IVY85_002839"
     CDS             complement(2988..3260)
                     /locus_tag="IVY85_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8045790.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3260..3523)
                     /locus_tag="IVY85_002840"
     CDS             complement(3260..3523)
                     /locus_tag="IVY85_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8045791.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1092 a          754 c          473 g         1400 t
ORIGIN      
        1 aaatgttctt actgcctctt ccacactaat ttcttcgttc aatttcacta ataatacttg
       61 atctcgctta ttataatcgc tttcatttat cctaagagtc aaatcttttt ccaccataac
      121 ttttacaccc aataaatttt gcggtttatt tttctctgtt gaaattcttt gtgtagcctt
      181 taaactgaca attagctcat tgggtacata ctctatttga gagtcatcta attggctcat
      241 aatcctttct ttttggttag tatctgcagt tacttgttta gtatgagcca tgaacccaaa
      301 catactcacc ccaacaacca atcctaaata tattttcttt ttaaactttt tcatctacat
      361 ctcctcctaa tagttttatt ttagagttag ctctattact tatttactaa ctcaacaaca
      421 gattatcata cacttgttaa tgaaatcaca atatctcatt aaaacacacc ctaaaatcat
      481 aaccgttcct atgctcattt tttggatttt ttaatcacgt aaccagaaaa ataaatcccc
      541 tctatttcac gcaaatcaaa gaagaactaa gtaactaccg tttaaaggta gttacttagt
      601 tcttctttga ttattatcta actttattat tcattcccca ccctttgcgc agtgagtctt
      661 cggtatcttg ttttatctga ctggtagttc ttattatttc tccacgatca tctaattctg
      721 gaacaattgg atatttttct ctgaacaagt ctagttcttt caccacgtca ttaaacaaat
      781 cttgcttatc ttcttgcgta agattctgtt taatatactc atctaaagct tttctaagca
      841 attcatctct tgtcatatta gtctcatact tttctactaa aactctggaa gtcgcattta
      901 acaaagtcga cagttttaaa ggatgcttgg tggaataaac cttatcaaac tttctgattg
      961 gtgcgccaac tttttttgtt cttcctctac tctgtctttc aacattggag gaagttttta
     1021 tttcttgaac ttggtgcact tctaattgct tattttcaga tggtgtaatt gcttgctgct
     1081 ttttctttgc ttctgcttct ttttttattt tggcgagtcc tttgttgttc ccaaaaatat
     1141 caagtccttt gatgtcagcc aattactcca cctccgcata ccaattctcg tgttcttcca
     1201 attctttcaa aatactgata aataattcgt ggcttttgcg atcccattgt tttaagagac
     1261 ctttctttgt cttctccatc gtaatacctg atccgtcgta tcgttttaag cgcgcatttt
     1321 tgagtacaac atttttaatc aaatgttccc cataaatctc ttgggcttgt ttatacattt
     1381 cctgatctac tgcgtcaccg ctttccaaca tgaaaggaat aacaccagca acatataaat
     1441 ttgtatcaaa ttctttcaca aaaaagtcca tatactcaag atattgcatc gcatttctca
     1501 tcgattttac ttgggtctgt aagacgacga ttgcccaatc cgcaatgtac atcgcggatt
     1561 tactatatac agaaatagaa ggtgggacat ctaaatagat gacgtcaaac tcatcaaata
     1621 atggttctag cttttctttc atatatttga attgggataa tgaatcatta tattggtctt
     1681 tgatccaatc atcatacatt tcaaaggaca tgtctgctgg aagtatatag aggttattga
     1741 ttactttata gacagcctct ttaggtggaa gattgtttat caacacgtca gtcacagtat
     1801 tttcgtaaac aatcggttca ccttctccgc ctccttcttc gttagtgaag ttcatatgcg
     1861 ttaagttcat cacatcagaa gtatttcctt gcatatccaa gtcaattatc aaggttcttt
     1921 ttccttcatt aactgctgat tcaaacccta acatttgcgc gcacgtactt ttacctacgc
     1981 cacctttaaa attaccgatt acatatttct tcgctaccac gttcctacct cttttctttt
     2041 agcaatattc atttgatact attataacat atttattttt agttactacc aaaaaaaaga
     2101 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
     2161 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
     2221 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa aagaagccgt
     2281 ccttcttaag aacgacaaca aatcactaga ttaatttttt caactgttca taaaagtttt
     2341 catgcgttcc acataacaat actggaataa ccttgttttc tataatctcg taagctactc
     2401 tatatgttgt gcccgcatat ttgaacccac tagaccaaat acctgataaa tccccagttt
     2461 tttgttcacc cttatatgga tcaatcgcaa tttcatcata aattaaattc aaaaattttt
     2521 ctttcaatgg cttctctttt agctttttaa atacttaatg acatgttttg aaggaacgat
     2581 ttcataagcc aatttctgtt gccaactcct ctttagtcat tactttagaa ttagtaagtg
     2641 tatcttcagc aatatctcta aaggaagaat gcattttgtt ttttctattc ttaaattcta
     2701 caagaatctc atctttatcg tagccttctg caattatatc agacaaaata tcttcgctaa
     2761 aatcataaaa ttctttttgt ggttcaacaa attcataaag aataccattc tctaccaact
     2821 ttgcttcaac ttcgactcca ttaggaacat taaaatcttt aggtactgtc aacatgattg
     2881 aattgccttg ttttcttgtt tttgtaatca tttaaatccc tcatttctaa cagtatgtaa
     2941 ttacttagta aatacattat accacgagat aatgtattta caagtaatta ctgtgactcg
     3001 taatgcgtcc ttgctgaata aatttcaacc acgtgttcat cttcatctac cttataaaca
     3061 acccggtgtt gtctattcaa tcttctagaa tacctacctt catgtgtagg ttttaacttc
     3121 tcaaatgatt gggtagggag atagggatct tcctttaaag tctgaataac cttctcaaat
     3181 tgaggtttca aattagactg tttgattttc tttaaatcag ctttagccga gtttttaatc
     3241 atcacagaat agttattcat tataagttat cccaatcaat ttcatcaaca ttagtaaatc
     3301 cactatcatc tttttctcgc ttacgaacaa cacccaaagt gcctatttgc tcaagtaata
     3361 gcgtttcttt gattgctttc caatcatcca aaccaataag caccgcatta ttttcactgc
     3421 gctcactaat gatttcaatt tccgtatgat tctcatttac atctttcaac aacttataaa
     3481 aatttttccg tgctgagcta ggatttaaga ctgtttttgc catatctact ccccctctaa
     3541 tttatatctc cattctaacg tacttaatta agtacgtaaa ttcgattgtt ttgtttaaat
     3601 tggagtacca aaacagcatt gtaaagtgtt atccgtttcc tttatacccg tggtgctagt
     3661 taattttttc ctagaggaaa atgtctaaac atgttggtaa atcaacaaaa aaacgcata
//
LOCUS       DACCYH010000153         3509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.162, whole genome shotgun sequence.
ACCESSION   DACCYH010000153 DACCYH010000000
VERSION     DACCYH010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.162"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(238..1185)
                     /locus_tag="IVY85_002841"
     CDS             complement(238..1185)
                     /locus_tag="IVY85_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP8045792.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
     gene            complement(1190..1942)
                     /locus_tag="IVY85_002842"
     CDS             complement(1190..1942)
                     /locus_tag="IVY85_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP8045793.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            complement(1943..2851)
                     /locus_tag="IVY85_002843"
     CDS             complement(1943..2851)
                     /locus_tag="IVY85_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP8045794.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            complement(2851..>3509)
                     /locus_tag="IVY85_002844"
     CDS             complement(2851..>3509)
                     /locus_tag="IVY85_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8045795.1"
                     /translation="KKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDW
                     HPWEDYRNFFVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSF
                     TLIIEHETGIVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDI
                     QLYDEVMHDNNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN
                     "
BASE COUNT         1155 a          655 c          431 g         1268 t
ORIGIN      
        1 caacttttac aatttcaaac tacgaattat gattcgtcat ggcaaaacat ttttaaccaa
       61 ataaacagaa cagaaagagg ttttcttctt tctgttctgt ccaaattatt ctaagatttc
      121 tctttaccaa catcatttga caaagagccc ttctatccca gatttgattt tacaaaactc
      181 atcagtcctt attgacaaag agcctgaatt taccctctaa tcttatctta gtatgtttta
      241 attgatttca gttttcaaat aattagtcat tccacttaaa ataccatatt tcggttgata
      301 gccaattgac tggatcttag taatatctgc tagagagtca tggatatcac ccgaacgctc
      361 ttctttgtat tcaagtgtca attctacacc gagaatttca ttgattgcat gaatcaattc
      421 caaaagtgtg gttgctttac ccgttccaac attgaattgt ttccccagtg cagcttcctc
      481 attcgcaact aaaagtaatg cttggattac atcatctata taaacaaagt ctctagactg
      541 gcttccatca ccaaataatg taaatgaagt tgcttctcct acgatttgtt tcttatagcg
      601 atctacgaga atagaaatca caccactgta tggagaattc ggattttgat ttggaccata
      661 gacattgaaa aaacgaactg cgctaccggg tacatcatat aaatgacaat aatctagcac
      721 atattgttct gcagcgaatt tatcaaccgc ataaggcgtc aatgggcgaa tcacggactc
      781 ttcttttttt ggtaaagtcg gttcatcccc ataaacagcc gctgaagaac taaacacgat
      841 ccgttttaaa tcaggttgat acttgcggat caattctaaa atcattaata cactttcaaa
      901 attcacacga tgtgtttcta ctggtcgggc aactgaatct gccacactag caactgctgc
      961 taaatgaaag atataatcaa atttattagc tttcatgatc tcttccatca aggatttctc
     1021 agcgacatct ccctcaataa aagtgatatt tttcgtcata tcaagatttt ctacttttcc
     1081 cattgataag tcatcgacga cgattaccac atgttcttct cctaaatgat tggctaaggt
     1141 agaaccaatg aagccagccc caccggtgat aagataacag ctcatccttt tacctccgat
     1201 ataaataatt atagatatct tgaagctccg aagaatcttc taaaaattta ggaaacaaaa
     1261 attctaacca tttaaaatct tcttcactat caatatctaa aattagataa tctctcattt
     1321 ttattatccc acattgccca tcaaaaatat aattactttt ttttagaaat tcaggtttaa
     1381 aagaatacat tgaggcattc atttcaaaaa cttcaggtgt ctgttgccta gatgtgtatc
     1441 ttgatttatt aactatagta acttcatttt caattctttt taccatatta aaatagggat
     1501 tacgtcttgc agaaacaact gaaaagtaga cgtctttatc taaatttttt agatgttcat
     1561 ctattaattc ttcaagatta gaaacttcac gtaaaggtga agtcaaatct aaatctataa
     1621 ttaaatcaaa tatattatct tctctttcta gttgtaagaa tgaatcacgt atcacttcaa
     1681 ttttccctac agtatctgta gctaattcag gtttacgttc tacaattttc acagacggat
     1741 ttaacttagt cgtcaattct actaactcat tactgtccgt attcaaacaa gtcgtgcaat
     1801 tatgttcagg atgtttttgt tgatagatat ctatcattgc taaagcgaaa tggactaggg
     1861 gctttcctgc caaatatttc aaatttttat ttttaaaccc cttagaccca gctctaccac
     1921 aaatagtaaa taaaatttcc atttatttct cctcagctat tttcaatact tcgtttgcat
     1981 gatataaatc attccagttt tctttgcctg ttactacgtt ttcaaagaaa tatttcaact
     2041 ccttctcata catatcatta gcagattcag gcatttcttt aacaatgttt tcttcaaaac
     2101 ttattttatt atttaataag tcaacattga tacattcatc taaagtaaac aattgaaatt
     2161 ttcttgtagg aactcttcca aaataatcta aatgaatttc tataatttta tcatcgtatc
     2221 tcaatatata tgttgctaaa tcattactgt taatttcaag attcgaatat ttaccaaact
     2281 gtttttttac tgatttaggg aaaccaaata ggtttataat ataatccaat tcatgtatac
     2341 agtctaattc tactcctccc cccatagatt taaaggcaga ataattattt ctataatcag
     2401 taccttttct ccaatcagga agataagtag aacaaatagc tcttacagaa tatacttttt
     2461 tactttgaat actattacga acaacttcca tgatattttt atacctcagt ggagcagcaa
     2521 cataaatatt tttgtaatca taattatatt gagtaagatc aatactctca gaaaaagcag
     2581 gtttttctaa aaatatgtaa tcaacttttt caacaattac atttagtgtt tccagatgct
     2641 taaaagtcgg atttgtaata aaagctatat cataatgtgt atccaaatcg tctctatttt
     2701 gaatatacgt tatttcttca aactcttttt ttccactgct actataagct gttatatcaa
     2761 aattaataga attctttttt ccatagctta ttagattttt tagatgtctc tttccaattg
     2821 accctaaccc aaaaaaaatt actttcatca ttaatttacc tcaatgtcgt taattaagtt
     2881 aataatatac ttatcttttt caaaagtata ttggggttcc attttctttt ccaaaagcgt
     2941 gaaaaattct ttaatctcct ctaaatatgc atcttcaata atagtatgtg aataattatt
     3001 atcatgcata acctcatcgt acaattgaat atctgtcata ctagaagttt cttcattcca
     3061 tgaaaataga gaatccgggt taccttccca ttctattaaa atcttttcac caattatagt
     3121 taaatttctt ttagctactc ttgaaacaac atctacactt aaagttccaa ctataccagt
     3181 ttcatgctca ataataagag tgaaactgtc cgaataatca atatcaagat tggataattt
     3241 ctgggtcatt acagtaaacg atttaacttt cccaaatgta tctataatcc aaggtaattc
     3301 aatagcgaat atttctctac acccattagt ttttttgttt gaaacaaaaa aattgcgata
     3361 atcttcccaa ggatgccagt cgggtaaata ctgtcctaca tggtattgat aagaaatttt
     3421 tttattatgc gtcactttag acttaatata ttgaatttct tttctcttca aaaatgtaga
     3481 agatagataa agtaatctat ctttctttt
//
LOCUS       DACCYH010000154         3254 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.163, whole genome shotgun sequence.
ACCESSION   DACCYH010000154 DACCYH010000000
VERSION     DACCYH010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.163"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            39..302
                     /locus_tag="IVY85_002845"
     CDS             39..302
                     /locus_tag="IVY85_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP8045796.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            296..646
                     /locus_tag="IVY85_002846"
     CDS             296..646
                     /locus_tag="IVY85_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8045797.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(670..1284)
                     /locus_tag="IVY85_002847"
     CDS             complement(670..1284)
                     /locus_tag="IVY85_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8045798.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            1598..2020
                     /locus_tag="IVY85_002848"
     CDS             1598..2020
                     /locus_tag="IVY85_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045799.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            2030..2233
                     /locus_tag="IVY85_002849"
     CDS             2030..2233
                     /locus_tag="IVY85_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045800.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            2444..3028
                     /locus_tag="IVY85_002850"
     CDS             2444..3028
                     /locus_tag="IVY85_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8045801.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         1124 a          482 c          592 g         1056 t
ORIGIN      
        1 tatgttatac tatgtatata cagaaagagg tgtctactat ggaaatgtta cctataaaaa
       61 aatggggaaa tagtaatggc cttagattgc caaagtatat tatggaatat cttggaattc
      121 atacagaaga caaagtgaag ataatacaag aagaagtaaa cggtcagaag cggctaatta
      181 tcgaagcggc taatatagaa aatgaattaa caattgaaca gttatttgaa aattacactg
      241 atgaaagaaa tcatgtgacg attcaaaatt taggtgaagc agtaggtaat gaaaaatggt
      301 gagaaaacct agacaaggcg atattctatt attgaataca gcacctcgat ctggacacga
      361 acagacaggt aaaagacctt atattgtttt aagtcatgat attattgcgg attatagtaa
      421 cgttgttaca gttgctccaa tttctagtac aactagaaac tatccgctat atgtaaatat
      481 aaactcagaa tatgaaatga agacaacagg aaaagtatta ttagatcaac taacgacaat
      541 tgattatgaa gccagagagt gtattttttt ggaaaaagcg catgatagtt tagttgagga
      601 gttattgatg aaagttagaa cagtttttca aaaagtaagc aaataaaata gaaacagcag
      661 catgatcaac tattcgtgat tgaccatgct gtcttttttt attctatata cggtttgtct
      721 cgtaacattg tagtcttttg caatcttagc gatcgcaaca ccttttttta gatcctcaac
      781 gatgttttta taaacaagac gacgttgggg atcttttgca ttggcactgt atagcttagg
      841 acgtccttta taaacaccgt tcgcttttgc aatcttaatg ccttgtgcct gacgtcgttt
      901 agattcggta cgttcttgtt cagcgatcat ggccaaaatt tgaatgatca aatctttaat
      961 gaatttatcc aggagaggat tgccaattgc ttcggccatg atcggtagac ttgtgatgat
     1021 cagctgcaca tttttcttct tcaagtaatt gactgaagcg atgatctcgt cgtaatttct
     1081 tccaagtcga tcaatagctt ccacaataaa aatatcttga tctcggacga aatccagtgc
     1141 ttcttgaaaa acaggtcgat gagtaatcgt tgcgccagat tgtttctcaa tgaaaattct
     1201 ctctgcaccg aattttttca tttcttcaat ctgccgttgt tcattttgat cgatggatga
     1261 gacacgtatg tatgcaactc tcaaaatttt tacctacttt ctgcaactat ttaaaaaaca
     1321 ccctaatcat acacgagaaa accagatttt tcaatgtcgc ggtcgtgtct agttagggta
     1381 taccttatat agacaaaaac taatgatgga ggaaatagta tgtgtcactt ttctttttaa
     1441 atggcaatat tttttgtaaa taaatgtcaa tcaagaaaat ttaaacgatt atgacattaa
     1501 aaatatattt tatgaatttt ctagtatata acttgattta tctgttaaga tttattaaga
     1561 aaacgttttc ttatttttga tttcgaggtg atagacaatg aaaaataagt ttattttatc
     1621 tttaggtatt ttaactattg gaattagtgt aactggaact acaattccta catttgctga
     1681 tagtacaaat gaaccaacta ctgtagtaga taataaccaa gaattaactc ctttagatag
     1741 gaacggaata gccattggta acccaactaa cgacttaact gttactgaaa attatttaat
     1801 agtttctcca ttacttgctc gagcagcttc taaaacgata cgagtgacag gaacgatgat
     1861 ttttgataaa gttggttggc cttctagacc tcctcaatca cggaattgga aagaaaaaag
     1921 aaatggttat tggtactatg gaaaattgta tttacaaagt tataaatcag ctggaaacaa
     1981 ttggattgcg acttattcag gaaccttaac tagaaagtag ggatttttta tgccagaatt
     2041 tgtgaacaca ccaaattttt atcaagatag cgatattcta agtaaaatta atttaccgaa
     2101 agaagaatca gctttacaag ttcagcgaac tttacaggag acacaaaaaa ttcaaaaatc
     2161 aatttctaat ctagaagatt ctgaagaaaa aagaaaaaaa agagaccaaa aaacatatca
     2221 aaaagaaaaa taatgatttt aaaaaaagag tgtctagtcg acattctttt tttctaattg
     2281 aaagtgttca taaaatcacc catttctgga cactttcttt ttttgcttta tttttcaaaa
     2341 aaacaattca tttcatgttc ataaacccat aacaatatct atgttcagtt aaactatatg
     2401 gataaagtta tgttacaata aaattaatga aggagtgatt tttttgaaaa taggatacgc
     2461 ccgggtatct actggtttac aaaatttgga tttacaagaa gacagcctaa ataaatttgg
     2521 ttgtgaaaaa atttttactg atcatatgag tggggcaaaa agtaatcgac caggattaga
     2581 aatggccatt gattttgttc gctctggcga tacacttgtc gtttggcgct tagatcgttt
     2641 aggtagaaat atggaagatt tgatttcaat cgtcaatcga ctgaatgagc gtggtgtgag
     2701 ttttcatagt attcaagaaa atattacgat ggacaaatca agttctacag gacagctcat
     2761 gtttcatttg tttgcggcat ttgcagaatt tgagcgtaat ttgatcctgg aacgatccgc
     2821 agcaggacga gcagctgctc gtgcaagagg acggtttggt ggtcgtcctg aaaaattgtc
     2881 tgaacaagat ttggaattac tgaagacttt agtggatagc ggtacaccta ttaagactat
     2941 tgcggaacgg tggaacgtct caagaaccac catttatcgt tatttggcaa aaatggagga
     3001 gaaaaatgga aagacaaatt ctcattagtt tttgtcattt aatgctgaaa atttttgcca
     3061 tttatctaga aaagtgacaa tagtatgaga attttttgta agtgtctact gataacttat
     3121 tctcaaatgt tgtaaggatt ttattcattt gttaatatta aagaacggaa ctccaaaaaa
     3181 gtccgtactc taatttgcag tctctcactc ttttagagtg agagttttta ttagattttc
     3241 aaattaatta gtaa
//
LOCUS       DACCYH010000155         3149 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.164, whole genome shotgun sequence.
ACCESSION   DACCYH010000155 DACCYH010000000
VERSION     DACCYH010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.164"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..511)
                     /locus_tag="IVY85_002851"
     CDS             complement(<1..511)
                     /locus_tag="IVY85_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8045802.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSL"
     gene            1164..1259
                     /locus_tag="IVY85_002852"
     CDS             1164..1259
                     /locus_tag="IVY85_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP8045803.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1561..1806)
                     /locus_tag="IVY85_002853"
     CDS             complement(1561..1806)
                     /locus_tag="IVY85_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045804.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            2151..2435
                     /locus_tag="IVY85_002854"
     CDS             2151..2435
                     /locus_tag="IVY85_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045805.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            2438..2854
                     /locus_tag="IVY85_002855"
     CDS             2438..2854
                     /locus_tag="IVY85_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045806.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
BASE COUNT         1051 a          589 c          492 g         1017 t
ORIGIN      
        1 ttaagctcct aaactcttca atacgaacta cataataacc agtagaacgg tactgcataa
       61 gcagtcggaa tagagttttt gcatacgtag aacgcaacga cgtgaaagca gataattcaa
      121 atctactaaa ttcagctgtt aaaccattaa taatatattc taggtccgga ttaacactga
      181 cttcaacaaa cttttttgtt ttattgattt caaaaccagt aaacaaaaca aagtagcgaa
      241 tagaatcctc atcttcagta cggtaagtaa gattaagcat atctttatag agcttttcta
      301 aatcaccgac aaaggctttt gtagcagtca tcttataatc acttaactct tttaattcct
      361 cgaaagaaaa tctaactttt ttcaaaccac gatctttcat acgagcgcat aatgcaaaaa
      421 ataaatccat ctgcttagca ttgaaatttt tcagcggtac taaattcaat tcattttgat
      481 atttgactgt taattccgtt ttttcatcca ttcctttctt ccccccttac aaataaataa
      541 taacctttaa caaccaactc gtaaataggt tggttgttat tatacaaaag gatattagta
      601 ctcagataaa aagttgttag tactcagata aaaagttgtt agtactcaga taaaaagttg
      661 ttagtactca gataaaaagt tgttagtacc cttctgtatc cgttgggaga gtaaggctca
      721 actgccccct aaaaagggtt ctaaaaaatc tttaaaaaat aaataaaata aacgcgcgcg
      781 cgcgagcaaa atttcccgcc aaaaaatcac ccacttttta aaataaacag gttcgcgcat
      841 gatattaaac tgccaatcta gctccctatg gtcgctgtaa gaaggagtga tcgctacgct
      901 ctcacatttt acttgcagca gtcgccttta ggctccttcg ccaaaaataa ttcattaggg
      961 actcctttgt ggcgtcctga gagctcgtct aagccgttct aagacaagat tcgataaaaa
     1021 ctatgtcctt cgttctaaag cgccttacag gcggtcaggg caagccctaa caaccccaag
     1081 gcaataaaaa acttaggctt atttttgctt ttttattgta taattgattg tcgcctatcc
     1141 gacagtcttg cgactggaag gaggtgtgtt tcgtgttcac taactttatt tgctcccttc
     1201 ttgttgggct tttaagcgct ttgttcagtt actggttgaa taaccgaaac aagaagtaaa
     1261 acttaattaa ggcgactcag cccacaaaaa aagccccaga gaatccgacc tctctggggc
     1321 tttcgtgtgt ttcgtgttca cttatttgca atcaaattat aacagaaata aatatttatt
     1381 tcaaaaatat cctacaaaag tctaaaatcc gtaattattt cttatatgca acatttacaa
     1441 ataaagtcgc tctgagagct cgtatttttc gatttaagac gttttattta gtttttggtg
     1501 tatttcattg gtccaagcta aaaaactctt agaagcgatt ttagagccat ttaaacccac
     1561 ttaattatat tctccatgaa attcgctcca tgcgtcaaaa gctgcgtctg aaattccgcc
     1621 gtcttcgttc actagttgca ttaatttttc caacgatcgt tttctttcgg gtgtcatatt
     1681 tttatattcc aacaattttt cgtctgttaa atcgaacttg attgcttgaa ttgcttgctc
     1741 taatttttct aagttttgaa aatattcgta aggaaatttt tccatttttt ctttctccag
     1801 tctcacaatt tttctagaaa taaaatcact tctttgccgt ctatactctc aaaaaaaatg
     1861 tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt atcgattaca
     1921 cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc actttctaaa
     1981 aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg atatcacttt
     2041 ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat acaaatatag
     2101 tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt atggacattt
     2161 tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc caagaactca
     2221 cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta atcgaaagtg
     2281 attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg ctcgataaat
     2341 ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac acaaataatc
     2401 aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt ctgtccaaaa
     2461 acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg aaaaaaatcc
     2521 agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg aacccgatac
     2581 caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag aaatttccat
     2641 catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag gcgttcttat
     2701 tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta aactaaatcc
     2761 atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag tcacttctag
     2821 aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt attcctatct
     2881 gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct tagtcggttg
     2941 taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat ttattcttct
     3001 aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa agtaaattct
     3061 aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag atcattgaag
     3121 tattttttta gttctcgtaa tgcaggatt
//
LOCUS       DACCYH010000156         3054 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.165, whole genome shotgun sequence.
ACCESSION   DACCYH010000156 DACCYH010000000
VERSION     DACCYH010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.165"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(597..1163)
                     /locus_tag="IVY85_002856"
     CDS             complement(597..1163)
                     /locus_tag="IVY85_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8045807.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1163..2119)
                     /locus_tag="IVY85_002857"
     CDS             complement(1163..2119)
                     /locus_tag="IVY85_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP8045808.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2119..2775)
                     /locus_tag="IVY85_002858"
     CDS             complement(2119..2775)
                     /locus_tag="IVY85_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP8045809.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT          958 a          592 c          545 g          959 t
ORIGIN      
        1 aatactgttt ctttttttgc agttacaggt tgtttatttt cggaaaaagc taatatagct
       61 aagattgaaa catccatctg gaatttatct tttttactgc tttgatcata aaagtctgca
      121 tattcatcac tgatattttt tctaataaat tcttccatca taaaaatcac ctcaaaacga
      181 ttatacgaac ttacgttctt gttgtaaagc gatatttgag gggcaaaaaa ggggcataat
      241 ttgactgata ttaagttagc tattgatata tgaatgacat acaaaaatat aaaatctttt
      301 aaaaatgctt atatatcaat gtttttgcca ttcttttata tatctgttat atgtcaaaaa
      361 aatgtgttca atttcaattt gtatttgaat aaagaggttg tgaaaaagct gtcctgcatc
      421 aagtaataag aacagccaat caaaaacagc ttctcatgtt tttgatttgg ttggacttat
      481 taccgcagat ataagctttt gagcaccgtg aataaagagg ttatgaaatc aacgctctaa
      541 cctgagtatt agagaaaaaa acgcaaaaca tatcatttat gttttgcgtt ttttatttat
      601 ctttttttct caatacaata taaaataggc ggctggttct tttggttgat aaaccgataa
      661 ttcagcacat tatatgtttc ctgtggcagt ttggaacaaa aagaatcaac catatccagt
      721 tctttttctc caccttcatg accatagtag acgacaagga tcaatcgtcc gcgaggcaca
      781 agtcgtttca atatttcttc catagctgtg cgtgtagtct ctggcatagt gatgattgct
      841 ttatcacttt ttgggagata ccctagattg aaaatccctg ctttgagatt ttgttgttca
      901 gctatcactt ctcctaacgt ttcgtggcct tgtagaaaca atgagacacg ttcttctaac
      961 tgacgctgtt ctagcctttc acgagtagca tcgattgctt gttgctggat atcaaaacta
     1021 tagacatgac cagtttttcc gatgtgttct gctagaaaaa ccgtatcgtg gccattgccc
     1081 atcgttgcat ctacgacgtg atcccctggt tgcaagattt cttgcagtaa tgtatggctg
     1141 aaatgcaaag cagtttgcaa catcagacgc tcaccttttt ccgtacatca tattttcctt
     1201 ggaagctatc tctgcgaaga agttcttcat caattgcatt caagacttcc cattttttca
     1261 agctccacat tggtccaatc aaggaatccc acggcgcatc ccctgtcaaa cgatggatga
     1321 tgatctcttt tggtatcatt tccagctggt cgcaaatgac attgacatag tcaggacggc
     1381 tcattaattg aaggcgacct tcatgatagt cccgcaacat tctcgttttg cgcatcagat
     1441 gcatcaaatg gagcttgatt ccttggatat ctgaatctaa aatggtgcgt ctgacatttt
     1501 ctttcatcat ttctaaactt tcgcctggta atccgttgat caaatgcgta caaacacgga
     1561 tattatgttt gcgtaattta gccacgccat ctaaataagt ttgataatca tgtgctcggt
     1621 tgatcagttt tgaagtttct tcaaaagttg tttgcaatcc aagttctacc cataaataca
     1681 tccgctcgtt cagttcagca aggtaatcaa ctacttcatc tggcagacaa tccggtcgtg
     1741 taccgactga taatcctacg acacctggta aattgactac ttgctcaaat cgttctttga
     1801 ttatttcaac tggcgcatgg gtgtttgtaa aattttggaa ataaacgatg tattgttgga
     1861 catgaggcca tttttgatgc atcatgtcga tttctttctg gaattggatt ggaagagggt
     1921 cttcaggtgc aacgatcatg tctcccgaac cagatacact gcaaaaagta caaccgccat
     1981 gggcaacagt cccatctcga ttcggacagt caaatcctcc atcgatcggc accttgaaaa
     2041 ttttcccgcc aaactcattt cgtaaagcgt aattccaaga atgatatctt ttatttcctt
     2101 ctgaataagg aaaagtcatt aaaagtcctt ctttctgtaa gagttatcta tttgttttct
     2161 tttttctcta tacactggat aagtcaggca cagccaagaa agactgagta agtaaccgcc
     2221 gatgatatca gtaggaaaat gaacgcccag ataaatgcgg cttatcccga cacttactat
     2281 aataaacatc atcagcaatt gaagcaatgt gcgtaaggtt cttttttgaa caaaagctgg
     2341 aagcagaaat agtaaagaac cgtaaagaat catactgcct gctgcatgtc cactagggaa
     2401 actgtaactt gtttcaacta ctaagtgcgc caatgaagga cgctctcgca tgaaaaatag
     2461 tttgatcacc gggttgatga ttccagcaat tacaataaat ccagaaacga accaaaatgt
     2521 ttctgccctt tttttcttat accacatcca taaagccgca caagcaaata aaatgatgac
     2581 ggtaagagga ttaccgaatt tagtcataaa gagttggtag ctgttcagat caggataaga
     2641 agctctgaca atgcctgtaa accactgatc gaaaccagaa agccacgaca gatggaactt
     2701 tacgatccag cccaaaatga aaaacaggac aagaaagaag ctaccagcca cctgaaatga
     2761 tgttttatat ttcatacaat tcctcctttg aagcaatgac ttattatagc atttttggtc
     2821 cagcgaaaaa aacggtaaag agataaaacc aatgtatgat tcctctttga ataaccaata
     2881 ttcttataaa aaaaataact gtaatcggaa agacattttt tggtaatgca aatgaaatgt
     2941 tagggaaata aaatcagtgt ttttcgaaat ccttttaaac aagaacgtag acgtaggaag
     3001 aacttgaaaa tgaacagaag ttgttcatta cggtaaatta caatatgatt acaa
//
LOCUS       DACCYH010000157         3020 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.166, whole genome shotgun sequence.
ACCESSION   DACCYH010000157 DACCYH010000000
VERSION     DACCYH010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.166"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            128..373
                     /locus_tag="IVY85_002859"
     CDS             128..373
                     /locus_tag="IVY85_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045810.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            471..692
                     /locus_tag="IVY85_002860"
     CDS             471..692
                     /locus_tag="IVY85_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045811.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            1014..1694
                     /locus_tag="IVY85_002861"
                     /pseudo
     CDS             1014..1694
                     /locus_tag="IVY85_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            1756..2373
                     /locus_tag="IVY85_002862"
     CDS             1756..2373
                     /locus_tag="IVY85_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045812.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT         1117 a          419 c          550 g          934 t
ORIGIN      
        1 acggtttttg aattctaaaa gttaaatagt ttataataga agaaaaagaa tggggtgacg
       61 atttcttaga ttgaaaagga aagataaata ttatagatta ttacttttga cagctgaagc
      121 aggatggatg gattttccag aagttgtaaa agaagttcaa gagataggtg caactatttc
      181 tgatagccgc tcctctccta tgaactatca ggatgcccat ggaattaaaa taattgatca
      241 agagagaaat gtcagaaaat acacaacaat aaatgagtgt gtaattaatg aaaatctttg
      301 tagggcaccg ataacgaaac acattaagaa tagaagcaaa gcaaaagacg gaagaacttt
      361 tactaccttc taagtttttt tcatatttgt ttgctatact tagatctaag gaattagact
      421 ttcgctgccc attatttaag tttgaggtag cgtcttgcga gggatcaata atggaagaag
      481 aaaaaatatt tgaaaaacgc tgggaattgg cttcagttga acaaagagca aggtaccata
      541 atctaatgtc ctcttaccgt aatattgatt ggacttacaa agaaaagaag tacttgcttt
      601 ggttatgtca gttagatgta aatacctttg aaacatttga agtgattcta gataagatta
      661 aaaacagcaa tgaaaaaaga gctgatctat aatggttcgc tctttttatt ttattaactc
      721 tttggttact atatgtaaaa tacagcttca tattacaata aatttcagga tacaaattta
      781 tttaagcata tataaatata attgaaatag gacataaata tatgagtatg agaaaggtga
      841 ttccatgttt aataaatggg caaaccaccc aatgaactcg agaggtagag ataatttgaa
      901 ggagacggac aagttctggg aagaagtata tagtaaaaac ggttctgttg caaagttttc
      961 caaaaaatct attttagtgt aaaattgaga aaaaagacag agaggacaga gtaatgaatc
     1021 attttaaagg caaacaattc aaaaaagacg tcattattgt cgctgttggt tactacctgc
     1081 gttacaatct aagctatcgt gaagttcagg aattgttata tgatcgtgga ataaatgttt
     1141 gtcatactac gatttatcgt tgggtataag agtacagcaa agtcctctat tatctttgga
     1201 agaagaaaaa tagacaatcc ttctattcat ggaaaatgga cgaaacctat atcaaaatta
     1261 agggacgttg gcattatctt tatcgtgcaa ttgatgcgga cggcttaacc ttagatatct
     1321 ggttacgaaa gaaacgggat acgcaagcag cctatgcttt cttaaaacga ctccataaac
     1381 agtttggtga gccgaaagca attgtgaccg ataaagcacc ttctcttggc tccgccttta
     1441 gaaagttaca gagtgtgggt ttatatacta agacagagca ccgaactgtg aagtatctta
     1501 acaatttaat agaacaagac catcgaccta ttaaacgacg aaataaattt tatcaaagtc
     1561 tccgtacagc ctcttccacg attaagggca tggagaccat tcgaggaata tataaaaaga
     1621 accgaagaaa tggaacgctc ttcggctttt cggtgtctac tgaaatcaag gtattaatgg
     1681 gaataacagc ctaagatatt tggagttcag agagggcgcg tttgattttc aaacttcgca
     1741 acagaaccga gaaccttggg ttttcaagtg ctttttggtt cattaatctt aaccatagtg
     1801 tttttaaagt ggaaaacaag caacaaaaaa agtatgtgga gccttgtagg aatagttctt
     1861 attattttta ctgtgactgc agcaacagaa gcaaattatt cgcataaaat tcagtcgtat
     1921 aaaggtcgtt tagttatgac caaaaaagat gttacacgtt atacatcctc aagaactgga
     1981 ggcctatatg actatgcacc attagatgca ttgaaatata aagatcattt aaagaaacat
     2041 gaagtaaaag ttggtgatcg gtgggagaat gcatctcata aagcatataa ttccaaaata
     2101 gtctatacag tagaagatgc cgatgagcaa aatatcacat tacctgtatt caattattgg
     2161 ggaacagttg tgaaagtaaa tgggaaaaag agcactatag aaaatgacaa tggtttagtg
     2221 tctattaaag gtacggggaa aagaactata gttgaggttt catctacgta tccaactacg
     2281 atgattctat ctttattaat tagttcaata acttttatat tcttatttac acgttattta
     2341 ataagatgct gcggtgaaat gaaaatagat tagatagata ttataaatat gttgattcta
     2401 ttttcatagc aatggagaaa atacattagc aatatataaa agaaggtatc aagagtcaca
     2461 gtgaatttat tccgaaaagt gaaaagagca aatataaaat ttaatgaaaa tatagaacta
     2521 aataggaaaa cgtagacgta attttctacg ttttcctttt catatatatt aaatattacg
     2581 gaaaattata taaactaatt tagtgttttg tttgtaattc ctaattttgc tccactttta
     2641 gataattttt aggtctattt aactcatcag acattctatg ctcatttttc tccaaatttg
     2701 tttcaactct ttcgtactta attttgcgta tttgaaaatt cttcttttag agtactttta
     2761 ttacgatact agtatgatat atatctatag caaacagtta aaatcttcct aaaataagcg
     2821 aatagcattc ctaaaaccta taaaattttt agatgaagcg acaagtggac aattaataaa
     2881 ctacttgaac atgaaattat ggatcacctt tcaaatttag aaaatggaat tcttatactt
     2941 attgcgcaac atttattgat tgcaaaatta tgtgacaaaa aaaactagta gaagaaagaa
     3001 taatggcttg ataataaagt
//
LOCUS       DACCYH010000158         2951 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.167, whole genome shotgun sequence.
ACCESSION   DACCYH010000158 DACCYH010000000
VERSION     DACCYH010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.167"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            569..2062
                     /locus_tag="IVY85_002863"
     CDS             569..2062
                     /locus_tag="IVY85_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP8045813.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            2197..2493
                     /locus_tag="IVY85_002864"
     CDS             2197..2493
                     /locus_tag="IVY85_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP8045814.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            2594..>2951
                     /locus_tag="IVY85_002865"
     CDS             2594..>2951
                     /locus_tag="IVY85_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045815.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILA"
BASE COUNT         1019 a          452 c          585 g          895 t
ORIGIN      
        1 atatacttaa tatattcgat atatctaata tactccaaat tgataagaat tcagacttta
       61 taaggaatat agtggatata ctaaatatat ttgctatatt cagtatatca gatatattca
      121 aaatgtcaaa atatatttgt gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact
      181 taattctata gaaaccacag ataattcact atttacaaag tagcaaaagc agtggtactc
      241 tttaaacaga aaaattacat aaaaaaaaga gacggaaata attgatttta gcagaatcaa
      301 ttatttctga atcacatggt taggacatgt gttcacgctc gtctcaagaa tatgtaagta
      361 gctaccaact acttatatta aaagttgatc ggataccttt tccgattgcc tttattctac
      421 caaaaaaagg cgagcaactc aacagaagaa cgtttattta gtgtacttaa attaaatatt
      481 cttaaacact gaagagagaa cacatactat ggctgagatt aaagctaaac tgtgttctct
      541 ttttgtttga gaaaggacgt tacaatcgat gttgagcgaa atttattcag ctattcttaa
      601 aaacggctta cgtacataca aatatcgaaa cagcaccatg aaacctgcag gtcatattga
      661 ttccagtagg agtggtgcta tctttgcgta ccgtagcaag caattaatga atgttgggcg
      721 tggtatggtc attacttcgg aagaagctat tttagagaac gaaaagaaac taacgcattg
      781 gacacctaat acctatcgct ttggtactta tgcggatgat tatcgaacag tggttaaagg
      841 tcattctgag aaaaacttaa gtcaaatcaa cacatttgta gttgatatcg attcaaaaga
      901 gaaccatcaa ggcgaaatta ttttagcctg tttagatcaa gtgggctaca tgccaacgct
      961 aattttagaa tcagatcatg gttatcaagt ttattttgta ctaaagacac caggctatgt
     1021 cacaaagaaa agcaatttca aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg
     1081 tctacagttg gctgaaaaat tgtccggagt ggatctagga tgtaaccact tcggaattgc
     1141 tcgattccca caagaaaata atattgtttt taaagaaatg aactatcagt attcttttgc
     1201 tgactggatt caatggtcga tgaagcttga atcagatcaa aaaacaaaag aagatcgaga
     1261 acgaaaacta attgtctttc ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt
     1321 cgatatgtta cttcataaag caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa
     1381 tgtgattttc acgctttctt tagcttatta ctcttcaggt tatgcattgg atacttgcga
     1441 atacaatatg ttcacattca acgaacggtt acaggaaccg ctatctgaag cagaattagt
     1501 aaagattgta cgtagtgctt attctggtaa ttatcaagca gcaagcagag aattcattat
     1561 tgaattgtgc cagcaatggg tttcaccaac cttaaaaaat aatgatctgt tcattcaacg
     1621 acgtggctgg tggaaattta aaaagccacg agatcaaaga aaatatagtc ataaaaatga
     1681 atggaaagaa gatctattgg cttatctaag cgaaaaaagt cttgctagtg agccttattt
     1741 gaacctgtca aaaaaagaat tggcttcaca actaacgatg cccaagcgaa cacttgataa
     1801 gatcttaaat gagttacgag aggagaataa agtcttctac aaggtaacac gaggaagaaa
     1861 tggcggaatg atcattgctt cagtcaaaac gttgttcgcg agggttattc aactaagtaa
     1921 agaaaagaga ctagattatt caacaaggat cacagaggta tttggactca acgagagctt
     1981 agtagaagag acatttagac aattatcaac ccccttgaac cctcttaaag aagtgaatct
     2041 ttttgaagta gatacaggtt gaaaaataaa aggtgcaaat tgccttatac aatatatcta
     2101 tgatacttct gtaggtgttg ctccctttgc ctagctacgt gtaggtggta tacttataac
     2161 tgcgtatgca gtcttatgta tcatggagga agttgtatgt cgttaaaaaa ttatgtctat
     2221 tcacatcctg ttaatgtgtt tatcattggt cgtttgggat tatctgtgga agtgttctgt
     2281 gagctttatg gttttaagca ggggactttg tcttcttggg ttaccaggga aaaaacagtt
     2341 gcttctttgc cgattgagtt tcttcatggg ttgtccttag cttctgggga gactatggat
     2401 caggtatatg attgtttgtg tgtgttagag caggagtata ttgagtataa aatagctaac
     2461 gagttaagaa aaaggaagaa gtatatccag tagtagtttt tatttactta aaaaattggt
     2521 tcattgagca acctatcgct gctaaagtag caaaggaaaa cgttaaagga aagaatttag
     2581 ggaagaaatc gaaatgaaat cggacgactg gattgaacac acctggcttc ctctgaaaag
     2641 aaatagcaaa cgaaagaaat tttgggtatt gcaattatgg ccattaggaa aactttgtag
     2701 ttacggggca cgacttattc ttgatttaac cgtttactcg attggagtat ttggaacggc
     2761 ttattacttg ataaatgatc ccaataggat tgcagcatca gacattttta cagtgctaat
     2821 gacgttgatt tttgtcatgc tattgtctat ttttactcaa ttagtcatgc aatttaccag
     2881 gcaaaaatta acagcaggaa aacttttcaa aagcctattc gatttaattg ttgtaaatgc
     2941 aattctagcg a
//
LOCUS       DACCYH010000159         2940 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.168, whole genome shotgun sequence.
ACCESSION   DACCYH010000159 DACCYH010000000
VERSION     DACCYH010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.168"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..1097)
                     /locus_tag="IVY85_002866"
     CDS             complement(<1..1097)
                     /locus_tag="IVY85_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8045816.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="IVY85_002867"
     CDS             complement(1227..2603)
                     /locus_tag="IVY85_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8045817.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..>2940)
                     /locus_tag="IVY85_002868"
     CDS             complement(2603..>2940)
                     /locus_tag="IVY85_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8045818.1"
                     /translation="ADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPII
                     LADEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKK
                     VNKMNLEVE"
BASE COUNT          893 a          564 c          413 g         1070 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acgtactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcagaatc tgctacttcc ttactatgtg
     2701 ttacaactat tacacattta tttctatctt tagctaatgt ctttaatata ttaattattt
     2761 ctccagcagt aacactgtct agattaccag taggctcatc agctagtatt attggagcat
     2821 ctgataccaa tgctctagca atagctactc tttgttgctg tccaccagat aatttcataa
     2881 catttctttt tatttgtttt ttatctaaac ctagttcaaa caagatactt tcatctgctg
//
LOCUS       DACCYH010000160         2887 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.169, whole genome shotgun sequence.
ACCESSION   DACCYH010000160 DACCYH010000000
VERSION     DACCYH010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.169"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            65..1657
                     /locus_tag="IVY85_002869"
     CDS             65..1657
                     /locus_tag="IVY85_002869"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP8045819.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            1650..>2887
                     /locus_tag="IVY85_002870"
     CDS             1650..>2887
                     /locus_tag="IVY85_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8045820.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQKM"
BASE COUNT         1043 a          462 c          591 g          791 t
ORIGIN      
        1 aagggagtat gctaaaataa gagggtatat agcaacttta aataagagat aggagtgtat
       61 ctttttgggt gcagaattaa atcaaaaatt atttagtgcg gcagataatc tccgtagcaa
      121 aatggatgcg tcagaatata aaaattattt attgggactg attttctata aatatttatc
      181 cgaccgtttg ttggaacaag tggttctttt agcagatgaa tccttagagg aatatgatac
      241 ggtaagcaaa caaacgatgt tgtatcgcga attactttca gatgaagaat caaaagaaga
      301 tctgattgca acaatcgttg atattttggg gtatgcgatt tcgccagagt atcttttcaa
      361 cgtcttagcg gatcaagcaa aacaagcaac gttccaattg aatgatctaa acaaagcctt
      421 tgtgcaattg gcatctacct acaatcaatt taatgggtta tttgatgatg tggatctcca
      481 atcaaaaaaa ctaggaacag atgagcaaca acgaaatgtg acgattaccg aagtcattaa
      541 gaaattgaat gacgtggaag ttttaggtca cgatggggac gtgatcgggg atgcttatga
      601 atttctgatc agtcaatttg cttcagaagc agggaagaaa gccggagaat tttacacgcc
      661 tcatatggtt tctgatatga tggcacaaat cgtgacgttg gatcaaaaag aacgacgatt
      721 ctttagtgtt tttgatccaa ccatgggttc aggttcattg atgttaaatg tgcgtaacta
      781 cttaactcac ccagataacg ttaaatatca tgggcaagaa ttaaatacaa cgacatacaa
      841 tctagcaaaa atgaacttga ttttacatgg ggtagatgcc gaagaaatga atctccgcaa
      901 tggggatacg ttgaataagg attggccaac agatgaacct tacacatttg atgcagtagt
      961 aatgaaccca ccttattcgg ctaattggtc agcagatacg accttcttag acgattctcg
     1021 gtttaatcgt tatggaaaat tagcgccaaa atccaaagca gattttgcgt ttcttcttca
     1081 tggtttctat catttgaagg aaacagggac tatggccatc gtcttgccac acggagtttt
     1141 attccgtggc gctgcagaag gggtcattcg tcaaaaatta ctagaagacg gcagtattta
     1201 tgctgtgatc ggtatgcctg cgaacttgtt cttcgggaca tcgattccaa cgacggtgat
     1261 tgtattgaaa aagaaccgcc aaactcgaga cgtattattc attgacgcta gtcgagaatt
     1321 tgtcaaagga aaaaatcaga ataaattatc ggaagaaaac attcaaaaaa tcctcgaaac
     1381 ctatgctgaa agaaaagatg tcgagaaata cgctcatttg gcaaactttg atgaaatcaa
     1441 agaaaatgac tataacttaa atattccacg ctatgtagat acgtttgaag aagaagaacc
     1501 aattgatatg gttcatgtcg gaaatgacat caagaagata cgacaagaac agcaagtgct
     1561 agaaaaagaa cttttagaag ctatctcttc cctgcaaaca acacctgaaa atgaagcgtg
     1621 gttacaaggt gcgctggagg tgttcaaaca tgagtaatga tatacaacca gaaattcgtt
     1681 tcccaggatt tacggaagct tgggaacagc gtaagtttga atgcttactt gataaaaaag
     1741 atggagtaag gaggggacca tttgggagtg ccttaaagaa agaattcttt gtatctaata
     1801 gcaattttgt tgtctatgaa caacaaaatg ctatttatga taattacgaa acaagatata
     1861 aaattactga aaaaaaatat aatgaattaa tcaaatttaa attagaacca ggcgatttta
     1921 tcatgagtgg cgctggaaca attgggagaa tttcaagagt accaaagcaa ataaaaccgg
     1981 gtgtattcaa tcaggctttg attagactta gaataaataa agaaatcaca gattcagaat
     2041 attttattca atttattcgt gcagatttta tgcaaagaaa actcacagga gctaatccag
     2101 gttcagcaat tacaaatctc gtaccaatgt cagaagtgaa aaaatggata gtacaatttc
     2161 caatattaga agaacaaaag aaaattggga acttcttcaa acaactggac gacactatcg
     2221 ctcttcaaca acgtaagcta gatttattaa aggaaacgaa aaaaggattc ttacaaaaaa
     2281 tgttccctaa aaacggagca aaagtgccag aaattcggtt tcctggattt acggaagatt
     2341 gggaacagcg taagttcaaa gagtttagta agaagactgg aaaaaagaac acgaaagatt
     2401 tagacttccc tgcctattcg gtaagtaata aagcaggttt aatttctcaa acagaacagt
     2461 ttgatggtag taggttggat gatttagaaa aaacaaatta taaattcgtt gaaccaaatg
     2521 aattcgccta taatccagca agagtaaacg taggatcaat tgcatttaac aatcttggta
     2581 tgacggtcat tgttagctct ctttatgtag ttgtgaaaat gagcgaggac ttagataatg
     2641 agttcatact tcagtttatc aagtcaccaa catttatcaa agaggtcaag cgcaacactg
     2701 aaggaagcgt acgagaatac ttattctatg agaatttcgc taatatcaag tttcctttta
     2761 ctaggaataa agaggaacaa caaaaaatcg gcgctttctt caaacaactc gacgacacga
     2821 tcgctcttca tcaacgtaag ttagatttgc tgaaggaaac gaaaaaaggc ttcttacaaa
     2881 agatgtt
//
LOCUS       DACCYH010000161         2869 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.170, whole genome shotgun sequence.
ACCESSION   DACCYH010000161 DACCYH010000000
VERSION     DACCYH010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.170"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            270..677
                     /locus_tag="IVY85_002871"
     CDS             270..677
                     /locus_tag="IVY85_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045821.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            691..1092
                     /locus_tag="IVY85_002872"
     CDS             691..1092
                     /locus_tag="IVY85_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045822.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1094..1465
                     /locus_tag="IVY85_002873"
     CDS             1094..1465
                     /locus_tag="IVY85_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045823.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1501..1803)
                     /locus_tag="IVY85_002874"
     CDS             complement(1501..1803)
                     /locus_tag="IVY85_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045824.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1899..2693)
                     /locus_tag="IVY85_002875"
     CDS             complement(1899..2693)
                     /locus_tag="IVY85_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HAP8045825.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKDPSEKRKLNISDHTAQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKRGVLLGTLTIDTLGKVLNVAVDKDTVDRISDSLNEIIYNLKR
                     RNISATNSNTSTISSASEIRKYKELLDDGIITEEEFNKKKQELLDL"
BASE COUNT         1016 a          410 c          433 g         1010 t
ORIGIN      
        1 agttgttgat atagttgcta atattgttag ctaatttgtt agtaaaactg tatacgaatt
       61 tagatagtaa aaaaacagtt tgttccttat atagaggcac ttttactact gattaaatgt
      121 cagatatgct tacaaatata cttataaata tgcttacaaa attagctaat atttccgttg
      181 acaaagcttt gttactattg ttaaactaaa ttgactagca aaagtgaaac ataaatttat
      241 taaataaatg taaaaattag gagaaatcaa tgtcttatag agtacaatta ataatttcag
      301 aagatgtaga aagtcagcaa tttggcacta atgttattaa taaagtaatt aatccagctc
      361 tttctattaa tgcgccttta attcctactg ctttatcatt ttctgtaaca gctgtggtgt
      421 ctgagataga ggatacagaa aagataaaaa tagtagaaat tgaagtttta aacaagaatg
      481 aaaaacaaat tttttctacg ggtgaagtat cggtaagctt gcctcctcaa gttaacgata
      541 taaactttaa tataaacgcc agaaatgtct tggttgaaga agcaggagag cattatgctg
      601 ttgttaaatt taatggaact gagattggaa ggcaaatctt tgatatcaag gtcaacaaac
      661 cagtggaaaa aaattaagga gacgatacgg atgcttgata tagtaccatc aaatacacat
      721 cggagtggta gcttaataaa ttttgctgcg atttttgctt gcgcatcggt aattctagca
      781 tctccagttg ttgtggatgc aagttctact ccgaaaacta aaaatgataa tcaacttgta
      841 attactacaa aaaatgagat taacacaatt tcaagtaatg atggcaaaat ttttgatgtg
      901 ataaatacgg ttttaaaaag aaaagctgaa atcaatataa aatatgatga agatttaaac
      961 ttgtatttct ttgttatcaa aacaacatct gaattgtttt cctcagatta cgatgttttg
     1021 gatactctcg ataatgtatt atctgactat aaatatatgg ggaaatctgt agtagcaact
     1081 ttgggggaat aaaatgtttg actttaatga atatttatct atttccaaac aaattgaatc
     1141 agatgataaa tataattcta aagaatctta tagaagaact gctgtttcaa gagcatacta
     1201 cagtgctttt aaaaaatctg atgagtattt gaaagaaaat tatgatatta tttataatgg
     1261 aagtagcgga aaaggaagcc accaaaccgt ttggaatttg ttttcaacag ttaaggaatt
     1321 gaatacctta ggaatacata atagtgggta tagaatgtta gagaaaagaa aatgtgctga
     1381 ctatattcct aatgaaacca ttacgaaaac agatatggcg ctgatgaatc gagaagcaga
     1441 aaaaataata aacaaactca cctaatgaat ttcaagcgag tttgtttgtt atttttttat
     1501 ttaatctttg tatctttctg gatcaacgaa agtatacttt atatagtcat aacgccgatg
     1561 atcgctacgt gcgtccggca cgtcagtcac gatatcaaac aaaaagtata cacccttctt
     1621 cattctagtt ttcgcagcag gaattttgaa atagttctta ttagaatagt agagattgat
     1681 taataagcta tcttcgattg ctaaaaagaa aacttctgaa tcccacacct tataaaaatc
     1741 tttgacaaat ctattcgaag gatcgaattt aaaccataat tgtgtttttc cttccattaa
     1801 catataaagt tcacctcaaa aagagtatac gaacaaatgt ttgtattgta aatagaaaaa
     1861 gatgagctaa attaaatgcc catcttcttt gatagatatt ataaatctaa taattcttgt
     1921 ttctttttat taaattcttc ttctgtaatg attccatcat ccaatagttc tttatatttt
     1981 cttatttctg aagctgagga aattgttgat gtattggaat ttgtagcaga aatatttctt
     2041 ctttttaaat tataaataat ttcgtttaaa gaatcactta tcctatcaac agtatcttta
     2101 tcaactgcaa cattcaatac tttaccaaga gtatcaatag ttaatgtacc caacagcact
     2161 cctctttttt tactaatatt atttaaattg tctaatataa tactttgtac attattgcct
     2221 atcatttttt gttgagctac aattaaacgt ttattagtta atgcgtatgc ataattattg
     2281 tcatgcttag ttgatgagat ataattatgc aatcctacaa agcagaagat tacgtattcg
     2341 tcactgttaa gttgatctga tattaagtta aaatgatgag ttgtccattt tttagtcatt
     2401 cctttaccgt agccgtattt aagacaaaat tctaccatct cttcagtggt tctcattcta
     2461 tcattaccta tatcatgatt gttttgtgca gtatgatcag atatgtttaa ttttcttttt
     2521 tctgatggat ccttatctaa atcttctaaa ttcaaagatc ttattatttt ataaggagta
     2581 gtcataccgt aaccagcttt tttcatacaa ttgccgcaaa caatttgttt atctttgatt
     2641 ttatatgaag ttaagccaat tttacctccg cactttgcac attgtttact cacattagtt
     2701 cctcctagat atcagctttc tactactatt aacattctat caatagtgac catactttga
     2761 ccatactttt tagaaagaat agaaaattcc aagatattta ataatagaag aaaagcacta
     2821 acatcatggg ttcagaagaa ttgagagctt atcaggaaac agtataatt
//
LOCUS       DACCYH010000162         2808 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.171, whole genome shotgun sequence.
ACCESSION   DACCYH010000162 DACCYH010000000
VERSION     DACCYH010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.171"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            143..1621
                     /locus_tag="IVY85_002876"
     CDS             143..1621
                     /locus_tag="IVY85_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP8045826.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRLESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
     gene            complement(1689..2780)
                     /locus_tag="IVY85_002877"
     CDS             complement(1689..2780)
                     /locus_tag="IVY85_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP8045827.1"
                     /translation="MLELNTSLSNRTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFIFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
BASE COUNT          997 a          500 c          447 g          864 t
ORIGIN      
        1 caaaaaaata attgaaaata cttaaaatca aaaaatatct ttttaatcaa taaaaaaatt
       61 gacagtttta ttaataataa gctattctgt tatagaagaa aaaattgttt gttattatta
      121 gcaacaaaaa aaggagtgca tcatgaaaaa gttatctcaa aaacgtttag ctcaaatcat
      181 tattgattct agaaaatctc tgaaaatgac acaacaacaa cttgcagtag ctactggtat
      241 caaccgtgcc ttaattagtc gattagaatc tctcgatttt atcccgtcaa tacaacaatt
      301 agaatcatta gcagaagttc taaattttga accaacagat ctatttataa atgaatcaaa
      361 taaatcaaac agaacaaaac atcagtcgaa gaatattgct gtagccggta ctggttacgt
      421 aggtctttcc attgctactc ttcttgccca gcacaatcag gtaacagcag tagacattct
      481 tcctgaaaaa gtaaaacaaa ttaacaataa gaaatctcca atacaagacg attacattga
      541 aaaatatcta gctgaaaaaa atcttaattt aattgcaacg actgatggtg cagccgccta
      601 tagagaagcc gattttgttg tcattgctgc tccaacaaat tatgatagta aacaaaatta
      661 ttttgatact tctgctgtag agtcagtaat caaattggtg ttagaagtca atccaaaggc
      721 aataatggtc atcaagtcga cggtacccgt aggctatacc gaaagtattc gtaaaaaata
      781 caatactaaa aatattctat tcagcccaga atttctcaga gaatctaagg cattatatga
      841 caatctatat ccttcgcgaa ttattgtcgg cacggaccaa actgacaaaa ctttacgaga
      901 agcctctcac acttttgcag atttactcca agaaggagca atcaaaaaag atatcgaaac
      961 tcttttcatg ggattcacag aagctgaagc agttaaatta ttcgctaata cttaccttgc
     1021 acttcgagta tcttacttca atgagctgga tacttatgca gaaatgaaag gactaaatac
     1081 acaagaaatc attcacgggg tttgcttaga tccaagaatt ggcacacatt ataataatcc
     1141 atcttttgga tatggaggct attgtttacc gaaagataca aaacaacttc tcactaacta
     1201 taacgatgtt cctcagaata tgataagtgc aattgtcgat agtaatcgta caagaaaaga
     1261 ctttatagca gatcgagtgt tagaacttgc tggcacgtat gaggctaaca gttcgtggaa
     1321 tgcagaaaaa gaaaaagagg tagtcatagg tgtataccgt cttacaatga agagtaattc
     1381 agacaacttc cgtcagtcat ctattcaagg agttatgaag cgaattaaag caaagggggc
     1441 aactattgta atttatgaac caacacttga aaataacact acattttttg gttcaaaagt
     1501 aataaataat ttagatgatt ttaaaaatat cagtcacagt attattgcta atcgttacga
     1561 tagtaatctt gatgatgtaa aagaaaaagt atataccaga gatttatttc aacgagatta
     1621 aaaaataaca cctcagattt gtagtgtctg aggtgtttct ataaactttt ataagtaaaa
     1681 aaatacaatt atacttcata aaaagactta taccactgcg caaatttacg aagcccgtca
     1741 cgcaaacttg tacttggttt aaattcaaaa tctcgttcta acgcactagt atctgcataa
     1801 gtaactggca catctcctgg ttgcattgga actagttcgg tatgagattc aaagtcgtaa
     1861 tcttctggta gcacccctgc tgcaattagc tcttgttgta gaatatctac aaaatctagt
     1921 aggttttccg gcgtattgtt accaatatta taaatatgat atggtgggat tggtagccca
     1981 tcttccccat caattttctc tggagcgcac tgcataaccc tctttactcc ttcaacaata
     2041 tcatctatat aggtaaagtc acgcttgcaa ttcccataat tgaatatttg gatattatca
     2101 ccagcacgaa gtttatcagt aaaaccaaaa taagccatat ctggacgccc agcaggacca
     2161 tatactgtaa agaaacgtaa ccctgtagac ggaatattgt aaagtttaga atatgcatgt
     2221 gccattgatt cattactttt cttagttgct gcatacaacg atacttgatt gtctacttta
     2281 tcctctgtag aataaggaat tttcttatta gtaccataaa cggaagacga tgaggcatac
     2341 actaaatgtt ccacaccaga ttctccatta tcatatgaat gacgacaagc ttcaagaata
     2401 ttatagaatc ctattatgtt agattcgata tacatatctg gattagtaat tgaataacgg
     2461 actcctgcct gagctgctaa attcaccacg atacttggtt tataattatc aaagatgaaa
     2521 tcaattgttg ctttatcagc aatagaacct ttgataaaat tccaagtaga tcctggatgg
     2581 ttctttgcct ccgtttctat ctctgataat ctatagttct taatagagac atcatagtaa
     2641 tcattcatat tgtcaatgcc aatgattttt attgatgact gagtacgtaa taactcaaga
     2701 accaaatttg atccaataaa accggctgcg ccagttatta atattgttct atttgataag
     2761 gacgtgttta attccagcat tttttagcac tcactttctt tttttctt
//
LOCUS       DACCYH010000163         2768 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.173, whole genome shotgun sequence.
ACCESSION   DACCYH010000163 DACCYH010000000
VERSION     DACCYH010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.173"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(75..1874)
                     /locus_tag="IVY85_002878"
     CDS             complement(75..1874)
                     /locus_tag="IVY85_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8045828.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(1998..2594)
                     /locus_tag="IVY85_002879"
     CDS             complement(1998..2594)
                     /locus_tag="IVY85_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8045829.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          976 a          522 c          480 g          790 t
ORIGIN      
        1 aaaacgcata tacctgcggt ggcaggttat gcgttttttg ttgtataaag acttttttca
       61 ttaacgattt ttgattagcc gactcccaaa acaaaattta gcagtttatc tgaataagtg
      121 atttcttcat ctgtttgttt cacactttca tttagtagtc ccatctcatc ggttatgata
      181 ccatccacac cataaaacat catgcgactc atagcatctg aatcgttgat tgtccataca
      241 tatacttctt ttccgtcttg gtgggcagaa ttaatgaagt tacggttgat cgtactatat
      301 tccattgtca gaaaatcagc aggcgtcaca gggggcccaa caatattgaa aggtaagatg
      361 tagcccacat aaaagtttgg ttctgcttct tttaaatctg aaacggtttg gtacgtcaaa
      421 ctttgcaaaa tatgatggtt ctctaagata actttgcggt atttatggac aaatcgttcg
      481 acaaggtcat ccgaatcttt tttagaggtc ttgatctcaa taagtaattt ttggtttagc
      541 ttatgtgctt tttccaaata agaatcaaaa gaacaaacag gggcttgcac tccgttttca
      601 gtaactgtaa gatttgtcaa ttctttcaat gtaagatcct gtggtgttcc atttacccct
      661 gttaagtgac gaagatttgc atcatgcatc actacgaaat gaccatcttt tgtttcctga
      721 acatccatct ctacataatc aggatgatag tctctgcttg ttttttcaag tgcttctaaa
      781 gtattttgcg ccccattttg ggtactcaca cctctgtgag agacagtcac tggttttgtc
      841 tgaacagctg aagtcagata atccatattg taaagacaga cgccaatacc gaaaaaaact
      901 gcaaaaagag tcaaaccagt gtttttcagc gcagagaaac gcaagttggg ggcttgtggg
      961 ataaaccact tagggatctc aggcaagaag ccttcgtcat ccatgaaatc aacaatgata
     1021 taaaaaatcc ctacggtaga catcacgata ttgaataata aaatcccttg taaaagcgtc
     1081 atagcaaaca cagcgctgat caatgcatag tctgggaaaa attgttcaac catactttgt
     1141 gccaaaataa caacgatata tcctaaagaa gaaaggagta aaatcgtacc gccaatcaca
     1201 ataaattggc ccgtgatggc caaaagacgc gatttggtca atgaccagct ttcccgaatg
     1261 gctgctcgaa aaggacgatc tcgtagaatc atttccggta aagcaaaaat cagtcgaatc
     1321 ccaatatagc caaggaaaat ataaacgagt aaaaaggaac tgacgatgat ccaacgatta
     1381 gcaaaaataa aatccataat gaaagcgggg atttttattt ttgacagtag atcagaatta
     1441 aaacttaatc cgctgatcgg tagaatcaat aaaaaataag caaggaaaaa caaaaaagta
     1501 atgggtctca ctttcttcag ctgaagtatc gtcaagtgta agagttgctt caaagagatt
     1561 ggttctttct ttttaataaa aaaaacactc atcaaaagaa aagtaaattc gaaataaact
     1621 aacagtaaaa tcgtcatcaa aactacgatt aacgagagta gaaccccagg atgttgagaa
     1681 aaaattgaag gaatattgtc ataagacaaa tagtcaattg cccctcggtg taaaataaaa
     1741 cgggtcgtac tgctcaataa gggtaaacaa ataaataaaa taaagccatg catcagcaag
     1801 acatcacgga aataagcttg tgttcctttt aaaaagtccc aagcattacc taagctacgt
     1861 ttcaaataat tcatcctatc acctgctctt cacattgtag cgcaaatcta atgaaaaagg
     1921 aaagggtgca gtgtgttttg cagacccttt cccagattta acattgtcaa tcaatgacta
     1981 attttacaaa ataaatatta tccaagaaga ccacttagat attgacctcc ataaatcaag
     2041 gccatactat ataagaaaat cgacagtggt ttagcaatca aaatgattag tgtatatcgt
     2101 ttcactgaca tgtttgtcaa tccagccatc aaacataacg catcgtcagg agcaactgga
     2161 agaaaaatag ctagagcaaa cagcttctca aaacggtttt gattatccag ccagccaata
     2221 tatttgttat atgtcttatc gctaatcatg gaaagtatga atggctttcc ataacgacgc
     2281 cctagcagaa aaatgataat cgaaccaatg cagattccaa tatagttata gataaatcca
     2341 gcataagggc caaatatcag gacaccagct gcagtactga tccctccagg aatgattgga
     2401 ataactactt gcaggatttg gatgaagaca aaaataatcg gtcctaaaat gatggaatca
     2461 cctactaatc ctcttaacgc attcaaatct ttgaaaacac ctaaattaat aaaataaatc
     2521 gtcagcgcga ttgataggcc taatccgata atggatatga aatttatcag ttttcgagag
     2581 gcagcaatat tcattattca tcaactcctt agaggttctt tgttaactat actttactag
     2641 aaaagcaact aactgcgcca ttttttgata tgacagaaaa caaacaattt tgtaagttca
     2701 tcataagtat aaaagattga tctcaaaaaa acatccaact aaagacttat ttaataactt
     2761 gttttttt
//
LOCUS       DACCYH010000164         2753 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.174, whole genome shotgun sequence.
ACCESSION   DACCYH010000164 DACCYH010000000
VERSION     DACCYH010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.174"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(16..1035)
                     /locus_tag="IVY85_002880"
     CDS             complement(16..1035)
                     /locus_tag="IVY85_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8045830.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGPNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            complement(1046..1243)
                     /locus_tag="IVY85_002881"
     CDS             complement(1046..1243)
                     /locus_tag="IVY85_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8045831.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            complement(1266..1559)
                     /locus_tag="IVY85_002882"
     CDS             complement(1266..1559)
                     /locus_tag="IVY85_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045832.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERSR"
     gene            complement(1596..1733)
                     /locus_tag="IVY85_002883"
     CDS             complement(1596..1733)
                     /locus_tag="IVY85_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8045833.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEAEKIINKDKSA
                     S"
     gene            complement(1735..2181)
                     /locus_tag="IVY85_002884"
     CDS             complement(1735..2181)
                     /locus_tag="IVY85_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045834.1"
                     /translation="MKNIWKYGRTGGEYAGQVLDDMVMTVPFTDVPPLEGIRADGEPL
                     TIADQMFDPKLNQWIVLVNVLDHNDLNNLKAMYESLENENGDLKQLNAKLMLSDVAIK
                     QENTALKEKADGLAQINSKTMLASFQNSKDIAEIKEQLNPASKGGE"
     gene            complement(2184..>2753)
                     /locus_tag="IVY85_002885"
     CDS             complement(2184..>2753)
                     /locus_tag="IVY85_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045835.1"
                     /translation="DWLKIEKGDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTE
                     KGLNDAVNVYDPQRVEGLKNFADGIQIAGDKVISENDCTVYTLTKDNSQSFIDGYATF
                     IKHGKEVIVNGTVKFKKAYAFGVPLDDEVPDEFVAKIVHGMLTGPSGTSSVSKAMYVR
                     KDLGTIITNSEFAANEWFTFHGNYWVGEE"
BASE COUNT          745 a          578 c          448 g          982 t
ORIGIN      
        1 aacgaggggc tatttttatc gttgcggaat atttaaatac cagcgtttat catgaaaatc
       61 ttgtgctccg cctttagtgt tcccttctgg atcattcgtt gcccgcatca ttacatagac
      121 tttcttatta gggaagttgc gcatgttaaa agatacatga tagccaacat ttcctaaagt
      181 attataagct tgatttacat ctggtctata tattccatca gcttttactc gagctaattc
      241 tttcccagta ttgtaatcca taatgaagat atactcgtat ttatagttag cgatgtgcca
      301 accagccaca tgtaagtttg cgttttcgat ttctccgaac tgatcaatgt gggcgtaatt
      361 tgttccatct gtcagggtag gatttgcagc acctgctcga gttggatcaa tgactggttt
      421 atcatctgaa gttgtcggat tttcatcggt aaatccatga gccaaatcat aggctaattt
      481 ttctttactt acgcccattt cagaaagata accgtaagga tctgtatgat cgccccaaat
      541 attttgtgtt acccataaat gcgatttgat ccctggttga ttatacggtg tgtctagtgt
      601 aagtggaata ccatatttca ttgctgaatc tctagctaat tcaacgtatg ccttgtagtt
      661 tttctcaaac gttgctttat catgtgtgtg ttgtaactca atctgcacag gactatttgc
      721 attagcatac gaaccagcac cgtactgtac ataaccaggt tgaccgactt gataaacaat
      781 tccgccgtct cccacaatat aagcagtata agcgctagtc catgaacgtt gcatatactg
      841 cgcttcattg cgtcctgttg ctgtttcatt agccgtttca tgcagtaaaa tgtactgatt
      901 atttgctact tgtgagctac cttcgtttgg gcctaaatta aattcattgt taatcgtata
      961 ggcaaaccca ttaattggca ataaaaaaag agccgttaat aggctcatcg cagtaatagt
     1021 aattttcttt ttcatttgtt tcctcctatt ttttcaaatt ataagccgac acgccagtga
     1081 taacgcctaa aaatgttgct actgcattga tagtgagtac tgtcatatct gttccattcc
     1141 atccatacgc tttgcctagt gttgcaacta atacagatgc agctggcaat actgttaaaa
     1201 ctgtccattt aatgacttga taatacttat cgggtaaaat catttcttct taactccttt
     1261 cttttttacc gagatctttc tctaaataaa gttttgattt gttgtgtgtg ttctaccaat
     1321 ttttctgtat gtgtatctaa tctttcatcg tgtttcttta gttcttcatg aatcatcaat
     1381 cgatctgatt tgctcgattc taaatcttta gtcagcaaat ctaaattgtg acttactttt
     1441 gaaagagttt cagtaatctt cgagaaagat gcagtgactg gtcttattac taataaaatc
     1501 aaagaaacaa tcgcagtgat tgatcctgcg attgttcccc attctcctaa attaatcatg
     1561 tgacaactcc tttaccttaa ataaaaacgc atcacttaag atgcgctctt atctttatta
     1621 atgatttttt ctgcttcttc gtctgtaatg catagtggaa cgaatagtcg aacttgatcg
     1681 tcagtaaaac agccccaatc atacatcatt ttcacatcgc taaaactaaa catactactc
     1741 acctcccttt gaagctggat ttagttgctc tttaatttct gcaatgtctt tgctattttg
     1801 gaacgaagca agcatcgttt ttgaattgat ttgtgctaaa ccatccgctt tttctttcaa
     1861 tgcagtattt tcctgtttaa tcgctacatc gcttagcatg agtttggcgt tgagctgttt
     1921 taaatcgccg ttctcatttt ctaacgactc atacatcgct ttgagattgt ttaaatcgtt
     1981 gtgatctagt acattcacta aaacaatcca ttggttcagt ttaggatcaa acatctgatc
     2041 agcaatcgtt agtggttccc catcagcacg aattccttcg agtggtggaa catcggtaaa
     2101 tggaacagtc ataaccatat catcaagcac ttgtccagca tactctccac ctgttcgtcc
     2161 gtatttccaa atatttttca tttttattcc tcccctaccc aataattacc atggaatgta
     2221 aaccattcat ttgcagcaaa ttcgctattc gtaataattg ttcctaagtc ttttcgcacg
     2281 tacatcgctt ttgatacact acttgttcct gacggacctg taagcatgcc atgaacaatt
     2341 tttgcgacaa attcatctgg gacttcatca tcaagtggta caccaaaagc ataagctttt
     2401 ttgaacttta ctgtaccatt tacaataact tcttttccgt gtttaataaa tgttgcatac
     2461 ccatcgataa acgattgact gttgtcttta gttaatgtat aaacagtgca atcattttca
     2521 ctgatcactt tatctcctgc tatttgaata ccatcggcaa agtttttcaa accttcaact
     2581 ctctgaggat catacacatt aacagcatca ttcaagcctt tttcagtata ttcaggtgtg
     2641 acatcccaac tgtaatcatt gggattgtta ctgtctttca agccttcacc aaagtattta
     2701 aactgactaa tattcggggt tcgtgtgtcg cctttttcga tcttgagcca gtc
//
LOCUS       DACCYH010000165         2688 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.175, whole genome shotgun sequence.
ACCESSION   DACCYH010000165 DACCYH010000000
VERSION     DACCYH010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.175"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            122..1129
                     /locus_tag="IVY85_002886"
     CDS             122..1129
                     /locus_tag="IVY85_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8045836.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1141..2145
                     /gene="neuB"
                     /locus_tag="IVY85_002887"
     CDS             1141..2145
                     /gene="neuB"
                     /locus_tag="IVY85_002887"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP8045837.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2142..>2688
                     /gene="neuC"
                     /locus_tag="IVY85_002888"
     CDS             2142..>2688
                     /gene="neuC"
                     /locus_tag="IVY85_002888"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP8045838.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNN"
BASE COUNT          984 a          341 c          530 g          833 t
ORIGIN      
        1 aatcaatcaa atgttgattc gttacgtttt gacatcatta ttgataatta gtttagttat
       61 atatttttta aagattaaga atcgaattat agatgactat aattcatagg aggagatata
      121 agtgtttaaa ggaaaaacaa tattagtaac tggcggaact ggttccattg gaagtgaaat
      181 tgttcgtcag cttttaaaat atgaaccaaa agctatcaga atttatagta gaggtgaaga
      241 aaaacaattt tatatgcaac aagagctctc aaatcacact aatttgagat ttttaattgg
      301 tgatatcaga gactctcaaa ggattgatta tgcatgtcgt gatgtggata ttatttttca
      361 tgctgccgca atgaaacacg ttccagcaag tgaatataat cctatggaag ctgtaaaaac
      421 aaatgtgatg ggaactcaaa atgtaataga tgcagcgatc aaaaataatg taaaacattt
      481 tgtaggtatc agtacagata aagtagtgag tcctactaac accatgggtg cgacaaagtt
      541 attgagtgaa aaattaataa tagctgccaa tcagtataaa ggaaaaacag aaacaatatt
      601 ttcttgtgta aggtttggaa atgtcatggg gtcaagtgga tctgttattc ctctttttag
      661 taaacaaata aaaaacaata aagatattac tttgacagat gttgatatga cacgatttat
      721 gatgtcaata ccggatgctg caagattagt tctgtctgct gctatgaatt caaaatttgg
      781 agaaacatat gtcttaaaaa tgccggcaat caatatttta actttagctc aatcattaat
      841 aaaaatatat aatgatacac atgatgaaaa atatagaggt gaaataaaag ttgtaggtat
      901 caggccagga gaaaaattat atgaagagct aatgactaca gacgaaattg aacgctcata
      961 tgaaaatgat aaaatgtata tcattccacc acactatcat gctactgacg aactgtacaa
     1021 agattatgaa aaaattaaaa tcactgcata ctcttcagat gcaacagaat taatgagtgg
     1081 acaggaattt attagaatcg caaaagagaa taatgtcgtg acttggtaag gagttttatg
     1141 atgaaatcag aagtatatgt aattgcagaa attggtgtaa accataatgg aagtctagat
     1201 ttagctttag aaagtattga taaagctaaa gaatgtggag ctaacgcggt taaatttcaa
     1261 acatttaaaa cgaataagct agtttccaaa aaggctccaa tggcaaaata tcagaaggat
     1321 aatcttaaag aggacagttc tcaatttgaa atgctaaaaa aattagaatt atctcaatct
     1381 gattttatta gaataaaaga ttattgtata caaaataaaa ttgatttttt gtctactcct
     1441 tttgatgaag aaagtgcaga ctttctaaaa caaattggtg tacaaggatt taaaattggc
     1501 tcaggagatt taacaaattt accttttctt agaaaaatag atatgtatgg gttgccagtt
     1561 ttattatcta caggtatggc tgatattcat gaggtcagtg aggctgtaga ttgtttcata
     1621 gatagtccag ttactatttt acattgtact tcaaattatc cggctagttc tgaagatatt
     1681 aatttattgg cgattaacac attaaaagaa atgttcggga tacccgttgg gtactccgat
     1741 cattcattgg gatatgatgt tggtatttgt gctgttgcga tgggtgctaa ggtaattgag
     1801 aaacatttta ccttggataa ggaactacct ggtccagatc ataaagcttc tttagatcct
     1861 caggaatttg cagattttgt aaagcatatt agaaacactg aaatattttt aggtgatggt
     1921 gttaagcgag caatgccttc tgaacttagt acaaaagaag tatcaagaaa aagcctagtg
     1981 gtcactgaga atttaaatgt gggagatatt cttacagaga aatctcttac aataaaacgt
     2041 cctggaaatg gaatagctcc taagttttta tatcaatata ttggcaaaaa agttaaaaga
     2101 ccaataagtg cagatagtgt tctgcttgag gaagatgttg aatgaaaaaa atacttatag
     2161 taagtggaac acgagctgat tatggaatat ataaacctgt tgctgagaat ttactacaag
     2221 ataattggga tgtatcattt gttgtttctg gaatgcattt atcccataaa tatggattta
     2281 ctaaagaatt gatcttagct gacggattta gaattagtgg agaagttcaa agtttgtttt
     2341 tagagaacaa taagggaaac atggctcggt ctgtggcatt agagataaat ggtttcacaa
     2401 atatatttga acaaactagc cctgattttg tattattact aggggataga ggagaaatgt
     2461 tggcagctgc gatagcttgt ttgtatcttg gaatcaaaac agcacatatt cacggaggcg
     2521 aagtgtctgg tactgtagat gaatcaataa gacatgccat aacgaaaata gcatctgttc
     2581 attttgctgc tacggaaaaa agcaaagagc gtttaataaa aatgggggaa gattcttgga
     2641 gagtttttcc cgtcggagct cctagaatag atactatttt aaataata
//
LOCUS       DACCYH010000166         2624 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.176, whole genome shotgun sequence.
ACCESSION   DACCYH010000166 DACCYH010000000
VERSION     DACCYH010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.176"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(879..1397)
                     /locus_tag="IVY85_002889"
     CDS             complement(879..1397)
                     /locus_tag="IVY85_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP8045839.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            2515..>2624
                     /locus_tag="IVY85_002890"
     CDS             2515..>2624
                     /locus_tag="IVY85_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8045840.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT          866 a          477 c          388 g          893 t
ORIGIN      
        1 tgttctttta ttaaatacac ctacaactta taaattgatg gtatttagat aacacgaaag
       61 aataaaaata acagctgata gtaaagtttg ttgacgattg ctactcaatc gaaagtgagt
      121 tagtgcgtat gaacttgctt ctgaaaactt atgaaatgaa agacctttta catcttgcag
      181 aaatcgttac tgggattaaa ctaagcaata aaaactaatg ttcccctcat tctttgaact
      241 tctactattt gatttattta ttctgataaa tgacgatttc accttcagga tcttcaaatc
      301 ccgtatagaa tttatttaag atctcttcag tatcagtaat aggataggcg ataggccaca
      361 taccctttga tggttagaat aaactgctca aaatctgtat cgtatgttgg ctttagcata
      421 ttaacaaagg ctatctccaa attataaacc ataagcgttg aatcgttctt aacaatttca
      481 attcttccta acccaaagtc agaagatata attaccggac ttgccttacc cttcatgtgg
      541 gttgttgaga ttccatattt aaagggaatt gtcggtacta tcggcggtct gaaaaacatc
      601 ccaaaaataa aaaaatcttt cccctgctag tctcctaagt ccctttgtag cggatatggc
      661 accataatct cattttatcg aagtgagtca tggtttatct gttggccaac caaaattgat
      721 acaatctaac gatgcaccct ctacggcatt ctaggggagt gcacttgcat tcacccaata
      781 aaaaccggca gttagcaaag aacttatgta gtcgtatgtt cttactgtta acatacaaga
      841 ggtctccttt ccacaattgc ttagcagtaa acgggaattt acaaagctaa tctcttccat
      901 tttcttatct ttgttctaaa agtcccaaat ggagcaactg tatttacatg aataaattta
      961 tatacttccc atacagccgt tttggttgcc tcgtctgccc attttctttt gtgtgcagtg
     1021 aatagatctt catcactcat tgaatcaatc atcatgttaa tatcatcaat attacgattg
     1081 agaatttcct ctaattcttt caatggtaga tgtgcatagg tatttgtaaa ccacttatat
     1141 aactctccta actgattcca tttaaattga tcagaaggcg ttttaacaga taatccattt
     1201 ctttcatcat tttcccattt tagaattaaa ttcgtccaac caacttgata tgctaaattt
     1261 tcagcaggtg ttctatccac ttcctcaact cttttatcct tcaattcttc aggaatagtt
     1321 tcaaattcta aaatatattt agtataagtc ctatttattt ctttttttaa ttcttcttta
     1381 tttttatatg ttctcattat attacctcca taaattccta attgtctcct ttaattcatc
     1441 tataatttca tccacgatat tttaatccgt tctacaatgc atatctgata tgcattgact
     1501 gtctaactca tatgtgaatg atttataaaa tatcccaacg tctcatctaa agaaatgttg
     1561 atcgtcagca aggagctaaa aaatacgaat atatttcttt tgtagtaaaa tcggactggt
     1621 ttgcatatca ttttcttcgt aaatatcaaa atcatgttaa ctactatcaa ttactaataa
     1681 aaactaccac tttaagcgtt aagaactgtt aagtttagtt aagtgaggat agttcacaaa
     1741 atccattgtt aaacttcacg aaagtggtaa aactcaaaat gctttatata ggattacagc
     1801 atcgctcttt cttcgattgc gcgttgggta aaaaatatta tgaagtaaaa attgacgatg
     1861 acacgttcct tacggctcat cagatgaaac agctacaaaa acgaaatgat caactcgaag
     1921 aaaatttaat tctaaaaaag cgattgccat attcacgcct cgctcgacga actattaaag
     1981 gtcaagattt acctgaagat catccttttt aggaaattga agctttttat gttgcacaat
     2041 gcatttataa tactacaatg atgttttcac cagatattct tctagggtgc ggtgtaatga
     2101 aacagataca cttgcaaaag aaaattcacc gcgaatttac taaaattatg aataattatg
     2161 ttaatcttcc gaaatttttt tccaaaatta agggatcaag ctggtgttat agattgttta
     2221 aggttggcaa aaaaataact tctttttaaa caattataga aaaccgatac tctgttaagt
     2281 tggttgacac ggtgtcgatt tttatgactt tcttattctg aaacttcact aacggacagt
     2341 acctaaaaag tctatagtat ggacgtttac gagtactatt ttatcaaaaa tgctgctcat
     2401 ttcaacttat atgttttagt gaactacccc cacttagctt ggataggcta caattaacta
     2461 gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct
     2521 aagagaacac gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta
     2581 gctggaaagc cacgtgtaga aatcattcga gaatatgaac taac
//
LOCUS       DACCYH010000167         2613 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.177, whole genome shotgun sequence.
ACCESSION   DACCYH010000167 DACCYH010000000
VERSION     DACCYH010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.177"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..561
                     /locus_tag="IVY85_002891"
     CDS             <1..561
                     /locus_tag="IVY85_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP8045841.1"
                     /translation="KFEKKKYVLQVFHPVVTELENIEKQILALINSMEQQSQPIICIL
                     PNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTILKECKFMIGNSSSGIIEAASFK
                     IPVINLGTRQNGREQSKNIINANLNENEIETALNKIQDPQFIKSLKNVKNIYGDGNSS
                     RRITKTLNQILNDGEDIKWIQKQIAY"
     gene            534..1211
                     /locus_tag="IVY85_002892"
     CDS             534..1211
                     /locus_tag="IVY85_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8045842.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1201..2343
                     /locus_tag="IVY85_002893"
     CDS             1201..2343
                     /locus_tag="IVY85_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP8045843.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            2356..>2613
                     /locus_tag="IVY85_002894"
     CDS             2356..>2613
                     /locus_tag="IVY85_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8045844.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDC"
BASE COUNT          976 a          341 c          510 g          786 t
ORIGIN      
        1 aaatttgaaa agaaaaaata cgttttgcaa gtatttcatc cagttgtaac cgaactagaa
       61 aatatagaaa aacaaatttt agctttgatc aattcaatgg aacaacaatc tcaaccaatt
      121 atttgcattt tacctaatag tgatgctggt agcgaaatta tcagaaaaat gtaccagaaa
      181 tatttaaaaa cggatattat ttatattacg aatttagagc cggaggaata cctgactata
      241 ttaaaagagt gcaaatttat gatcggaaat tcttcttctg ggataattga agctgcatca
      301 ttcaaaatac cagtaataaa tttaggaacc agacaaaatg gacgagaaca atctaaaaat
      361 attataaatg caaatctaaa tgaaaatgaa attgaaactg ctttgaataa aatacaagat
      421 cctcagttta taaaaagctt gaaaaatgtt aagaacatct acggtgacgg caacagttct
      481 agaagaataa caaaaacatt gaatcaaata ttgaatgatg gggaagatat taagtggatt
      541 caaaaacaaa tagcttatta aaatatacta gtgatccata cattattatt ggggctggcg
      601 gacacgcaaa agtcattatt gaaaccttaa gattagaaaa taagttaata tatggatttt
      661 ttgatgataa gcaaaataaa atgcaagatc tgaaatatct tgggaaaata aacgaattga
      721 atagctgggc ggatagcaaa aaaaaaatct ataaatttat tgtagctatt ggtgactgtg
      781 tagtaagaaa aaagattata aaaaaattga atatgccaag tgagcaatat ggaatagctg
      841 ttcatccaag tgctatagtg gcaaaagaaa tttcaattgg ttatggaact gttatttttg
      901 cgaatgctgt aattaaccca gatgctgtaa ttggagagca tgctataata aacacaggta
      961 gcattgttga gcatgattgt agaattaata actatgttca tttatctcca ggtgtatgct
     1021 tagcgggtgg agtacatgta ggtgtaggaa ctcaaatagg aattggttct caatgtatcc
     1081 agatgaagaa aattggatct tggtgtatga taggcgcagg gagcactatt gttaaggata
     1141 ttccctcaca ttccttagct tatggcaatc cagctaaaat taagaaagaa ggaattaatt
     1201 ttgaattcta gaattttatt atcaagtcct catatgggag gaacagaaga aaaatatgtt
     1261 gctaaggctt ttaaagataa ttggatagcg ccattaggtg aaaatgtgga taaatttgaa
     1321 gaaagtatta caaaatatac tggttctaag ggcggagctt ctgcgttaag ttcaggtaca
     1381 gctgccatac atcttgcctt aagactgctt aatgttggaa aaaacgatat tgtctttgct
     1441 ccttcattta cttttatagc tacagtaaat cctattttat atcaaggggc tgtaccagtg
     1501 ttcattgact cagaacctaa tacatggaat atgagccctg ttgcactaga gagcgcatta
     1561 gaagaagctg aaagaaacaa ccaattacct aaggcaatca tcgtagtgaa tttatatggt
     1621 caaatggctg attatgaacg aattttagct attgcaaacc aatttaatgt tccagtgata
     1681 gaagatgcag cggaatcgtt aggttctact tataagggaa tttcgtcagg cttatttggg
     1741 acaattggga tctattcatt taacggtaat aagatcatca caacctcagg tggaggagct
     1801 ctagtgtcta gtgataaaaa atatgttgag cattcaagat ttttagcaac acaagcaaga
     1861 gatcaagcac cgtattatca acatagcgaa ataggttata attaccgtct aagcaatatt
     1921 tctgccggta tcggacgtgg acaaatggaa gtattgaatg atagagtagc taaacgaaga
     1981 gaaattttca atcgatataa ggaagctttt tctggaattg atagaattga aatgatgcca
     2041 gaagtggatg gcatggtgcc aaatcgttgg ttgtctactt taacgatcaa tgattataat
     2101 tctattgatc aagtagttaa cgtaataaat aaactaaatg aaaaaaacat tgaaacaaga
     2161 gctttgtgga aaccactaca tttgcaacca gtatttcaag gggtaaaaat gtattaccat
     2221 gataataatc ttagtgtgtc agaaaagtta tttaatagcg gcttatgctt accttctgga
     2281 agtaatatga cagatagtga acaagaattt gttattgatg agtttaaaaa aatattcaag
     2341 taggtgtgat attttatgaa cataggaatt attggtttag gttccatggg gaaaagaaga
     2401 cttagactca ttcaagaaca ctttaatgat attttactat gtggtgttga tactaatgag
     2461 gcgagacgaa aggaagttga aaaggaattt ggaataccaa cttataatag tctaacagag
     2521 gcaaaaaatt tgtttgaaat aaattcagtt tttgtttgta catcccctat aacccatgaa
     2581 tttattataa aagaggcttt aaatttagat tgt
//
LOCUS       DACCYH010000168         2483 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.178, whole genome shotgun sequence.
ACCESSION   DACCYH010000168 DACCYH010000000
VERSION     DACCYH010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.178"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            241..888
                     /locus_tag="IVY85_002895"
     CDS             241..888
                     /locus_tag="IVY85_002895"
                     /inference="COORDINATES: protein motif:HMM:NF014505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8045845.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            1171..1244
                     /locus_tag="IVY85_002896"
     tRNA            1171..1244
                     /locus_tag="IVY85_002896"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1205..1207,aa:Ile,seq:cat)
     gene            1790..2356
                     /locus_tag="IVY85_002897"
                     /pseudo
     CDS             1790..2356
                     /locus_tag="IVY85_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
BASE COUNT          893 a          352 c          453 g          785 t
ORIGIN      
        1 ttgaggggaa gtacataatg caaatttgtc cataacttat tgattatctt gtaaaaatgc
       61 ggtaatttaa tagttagtga gaagaggtgc ttaattatag ttaaaaaaga ggaaatagaa
      121 gcagcatgta accaagcaaa tgttaggttt attggagaag ctttatctgg atatcctaag
      181 tataaatact tacacaactg gttacaaagt aaaagttttt atcttaataa agaaagatgc
      241 atgcctaata aaaattatag ggtttataaa agaggaacta ttgtatatgt tgattttggg
      301 gtgaacgtgg gttatgagtt atctggtaat cattttgcta ttgttttaaa taataaagat
      361 aattcgaaaa atggtgttgt atgtgtagta ccaatctctt caaaaaagaa aagtaattat
      421 gtatctgtag gaaaaatatt agaagttgct agtattaaac aatttataag ccaagctgat
      481 aaattaaaag agaaacttag gataattatt ttactttcat taaatagagg catcattgat
      541 gaaagcgatt tgaatagttt tattaaagat attgaagcta aaggtgaaaa gctttcagtg
      601 gaagaagtag aaaaacgagc tgtaaaacat aatttggttt gcgagacaag ggaacaagta
      661 attaaagaaa taaaatcatt atctgaagat atggtaaaat atcagaaggt gtttaattgc
      721 tacaagaaat atacacaaga atcctatgta atgccactta atatccaaac gattagtaaa
      781 aatagaatta gaagaattaa caagtttgat ccttctggaa atatgaaggc acctggaaat
      841 gtcctcaatg aagtagatga aagtttaaaa aaatatttta caaagtaaac gatatttttg
      901 ttgacatttt tctgttcgtt agttaccatg tagttataga aggacgtaag cgcctatttt
      961 taatgtacac atcgggacgt atgcgcccac atagaaacat agttgagaga catctatatg
     1021 gtgtctcttt ttttattaat atgcaaaaaa gtctagtttt ttcaataata tttatgtaat
     1081 aataaattag ccaccacaac aaagaatgaa acccattatt atctagtcta tgtccattct
     1141 ttttgtttgc agtagttgtg atggctttcc gtactcttag ctcagttggt tagagcagac
     1201 ggctcataac cgtccggtcg taggctcgag tcctacaggg tacatactct aagtgattta
     1261 aattgcgaat aaaatataaa aatgactccc cttttgactc ccccaagcag tatatggatg
     1321 tctctaaaag ttgttttttt agataataaa aagctgataa taagcgaaaa acacttaaaa
     1381 tcagctttaa accctattct cgaaatccgg cgaaccggct aataccggcg cgcaggttca
     1441 aatcctgtac cttccttata tcaacgatta gaggttgtga agaagctgtc ctgcatcaag
     1501 taataagaac ggccaaacaa aaatagcttt tcctattttt tcgtttgatt gggcttatta
     1561 ccgcagatgc aagcttttga acaccgtttg ttcggaataa ggggctgtga caagactttt
     1621 gtcacagccc cttattgttg tacttttaac ctacaatatt ttttgattcc ccctgagtac
     1681 tattttttat aaatgctagt aaatcaatct tctttggcct ttttttgata aaaaaaatac
     1741 tagatgtaag ataaaagtgt aaaaacgatt tgttttagga ggggaaacga tgtgtaccgc
     1801 aatcacttat gcaacgaaag accattattt tggaaggaat tttgattatg agatgtctta
     1861 taatgaagtg gtcactatta ctccaagaaa ttatcgtttc gattttagaa aggtaaaaaa
     1921 tttagataaa cattatgcaa tgataggaat agcagctggg gtatccaact atccactcta
     1981 ttatgaggct acaaatgaaa aagggttgag tatggcagga ctgaattttc ctggcaatgc
     2041 tgattataaa gaattacaag aaggaaaaga taacgttgca ccatttgagt ttattccatg
     2101 gatactaggg caatgctcaa ccatagacga agctaaagaa ttattggcta ctatcaatct
     2161 agtcaatatc gattttagtg aaaaactacc cttgtcacct ttacactggt tattagctga
     2221 taaagaaaaa tcgatagtaa ttgaaagtat gaaagatgga cttcatttat atgataatcc
     2281 tgtcggtgtg ttgacaaata atccgccatt tgattatcaa ttatttaatc tgaacaatta
     2341 tcgctcgcta tcaaatggaa cgagagtata aaatattttg tgtaaatgaa aaaatccata
     2401 caaaaaagga agtcccttct gtagaataaa gttaacgaca accaattcac agaaaagagg
     2461 acttccctat gaatgatttt act
//
LOCUS       DACCYH010000169         2400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.179, whole genome shotgun sequence.
ACCESSION   DACCYH010000169 DACCYH010000000
VERSION     DACCYH010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.179"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            207..2069
                     /locus_tag="IVY85_002898"
     CDS             207..2069
                     /locus_tag="IVY85_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8045846.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="IVY85_002899"
     CDS             2069..>2400
                     /locus_tag="IVY85_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8045847.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DACCYH010000170         2395 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.180, whole genome shotgun sequence.
ACCESSION   DACCYH010000170 DACCYH010000000
VERSION     DACCYH010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.180"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            316..570
                     /locus_tag="IVY85_002900"
     CDS             316..570
                     /locus_tag="IVY85_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8045848.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="IVY85_002901"
     CDS             571..888
                     /locus_tag="IVY85_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8045849.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="IVY85_002902"
     CDS             1447..2382
                     /locus_tag="IVY85_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045850.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DACCYH010000171         2381 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.181, whole genome shotgun sequence.
ACCESSION   DACCYH010000171 DACCYH010000000
VERSION     DACCYH010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.181"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            78..2231
                     /locus_tag="IVY85_002903"
     CDS             78..2231
                     /locus_tag="IVY85_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP8045851.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          813 a          422 c          529 g          617 t
ORIGIN      
        1 ccgcccattt tttctttttt attaagaagt ttttgggtac aatgaagcat gaatgtaaat
       61 aggcggaagg ataggaaatg atgaaagatt ttatgaaaaa aatattaaat acttcttggt
      121 tagaaaaaat aagaaaaggt gcacaagaac ctaagaacaa aacgtttcgc cggtcccatg
      181 ttccttttcg cttaaatttt ctttttttca ttatatttac gttatttgtt gcgttgattg
      241 ctagattggg ttatttgcag atcgtgaacg gcgaagagat ggaggcgcga gtgaagtcta
      301 cttctacgat tacggtccaa gaaagtactc ctcgcggaat gatctacgac tccacaggaa
      361 aagctttagt tacgaataaa gcgaatcagg ccatcacata tacgagagga acacaagtta
      421 cggctaaaga tttgcttgaa atcgcaacga agctgaactc gctgatcgat gttccggtag
      481 atgaaaatct gacagataga gataaaaaag atttttggct ggctgatccc aaacatttga
      541 aagaagccac aaaaagactt tcggcaaaag aaaagcaatt agggaacagc gaacaatata
      601 gtgcgatggt caataaagtc acagctgatg agattaattt aaacgaggag caattaaaaa
      661 ttgctaccat ctttaaacgg atgaatagtg catatgaatt aaacactgtc tatatcaaaa
      721 actctggtgt tactgatcaa gagttagctg ttgtggcaga acgcgcttct gaacttccag
      781 gagtttcgac tggcacagac tggacacgag aatataatgc agcaagttca ctgaaaagta
      841 ttcttggcag tgtgacgact gaaaagcaag gacttccagc agatgaagca gagaaatacc
      901 tagcaaaagg ttattctcgg aatgaccgtg taggacaaag ttatttggaa aaacaatatg
      961 aagatgtcct gcaaggaacc aaaacccaat atgaagtttc ccttgataat gaaggaaatg
     1021 tttcgaatca aaaagaaatc ttctctgggg aaaaaggttc taatttaatg ttatctatga
     1081 atgcagaatt ccaatcaaaa gtagaagaaa ttttgaaaag gaattatcaa acattgatca
     1141 acaacggcaa agcacagtat tcgcctggtg cttatgcagt ggctatgaat cctcagacag
     1201 gagaagtgct agcaatgact ggtttcagtc atgaacaagg ttccaaagaa atcaccgaga
     1261 atgctttagg cacgattact agcgcctttg atccagggtc tgtagtcaag gctggtactt
     1321 tgacagcagg ctgggcaagt aacgcgatca gcggaaatca agttttgatt gatgagccga
     1381 ttcgtctgca aggtgcttca gaaaaaagct ctgtctttaa tcgaagcgga caagttgctc
     1441 ttgatgcagt caaagcatcg gaattgtctt ctaatacgta tatgatcaaa gtggcgttaa
     1501 agatgttagg actagactat acacctggaa tgggactacc tagtttagat gaagaagcta
     1561 aagcttatca gcaattgaga gactctttca aagagtttgg tcttggtaca acgactggga
     1621 tcgatttgcc gaatgaatct cctgggatct cacgttctgt tgattatatg aagaagttca
     1681 atgcggataa tggcaaagaa tggtatgctc caggtaactt taccgatttg gcatttggac
     1741 agtttgacac atatacacca atccaattag ctcaatatgc ttcaacagtc gctaatggcg
     1801 ggaaacgagt acagccgcac ctagtcaaag cgatctatgg caatgacgaa aatggaaact
     1861 tgggtgaagt gaagaaagaa attggcacaa cagtagaaaa tacggtcaat atttctgcag
     1921 aaaatatggc gatcctccgt gaaggattcc accaagttgt ccatggtacc gatccttaca
     1981 caacagccaa accattagcc agtgcgaaaa tggacctttc cgcaaaaaca gggactgcag
     2041 aaacggttgc ggaaggtcat ccagatattg cgacagtcaa tagtaatatc gtggcatacg
     2101 gaccaacaga caatccggaa attgccatca gtgttgtctt gccgaactta ttagacgaac
     2161 aagaccacat aaacttgacg attgctaaag aaataatgga cgcgtattat gatatgttca
     2221 tggcaaaata aaaaagaggt cgtgaaaaag ccgcttagtt tcgagtatga agaaaaattt
     2281 tgtgaaaata gcttctcaca tttttgctta aatttgactt cataccgaga aactggcttt
     2341 tgaacaccgt ttactcggca aaaaaggttg tgacaaaaat t
//
LOCUS       DACCYH010000172         2172 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.182, whole genome shotgun sequence.
ACCESSION   DACCYH010000172 DACCYH010000000
VERSION     DACCYH010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.182"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            278..787
                     /locus_tag="IVY85_002904"
     CDS             278..787
                     /locus_tag="IVY85_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8045852.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="IVY85_002905"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="IVY85_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8045853.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
BASE COUNT          757 a          379 c          418 g          618 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca ca
//
LOCUS       DACCYH010000173         2110 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.183, whole genome shotgun sequence.
ACCESSION   DACCYH010000173 DACCYH010000000
VERSION     DACCYH010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.183"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            929..1402
                     /locus_tag="IVY85_002906"
     CDS             929..1402
                     /locus_tag="IVY85_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP8045854.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1411..1638
                     /locus_tag="IVY85_002907"
     CDS             1411..1638
                     /locus_tag="IVY85_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8045855.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          681 a          340 c          312 g          777 t
ORIGIN      
        1 acaataaata actttagtta ctttaatgcg ttcttacttt atttttatat tatcattttt
       61 cttattcact tctttggttt atactattct tataaataaa aatgaggtat gtcctatgat
      121 gaaaagattg tactattcat tgatcataac gattggctat ttgattgtat caaatctagg
      181 aaatatggta tttggtatca gtaaagaatt cagttggact actacattat gggaatctct
      241 attctttttc atcttcgtat ttttgttaca aaattatcga aaaaaataaa catgtgccat
      301 ctatctgttg gcacatgttt taggatttat ggtacttttt attcggcaat ccattttatt
      361 tttctacttc ttcttaatac ctctaacact tttgatttat aaactaatcc aatgcatcta
      421 cttgcgctta ttaagctatt tcagctagat agtaccaccc catgcgtcaa tgcttgattt
      481 tccttttgta taacattgta taagcaataa aggacaaata agtggtgatt acaccaaacc
      541 attgaataag aaaaattctg atacgtaaat aaacatgaac ttgaaaaaat taatgaatcg
      601 gttagctact acgtagttgt cttttttctc atacagttct gttaacttac acttctcttc
      661 gctagataat aaaaaatcct ccaactgtat tgtccaatat aatcccaggc atctaaacta
      721 gcattataga agagacacac atagaatcta aaataactgg ttttaaatgt ttcttacaat
      781 caaaaaaagc tgttaaatgc actccacacc ttcatactct atcatttttt ataaacgata
      841 tatttttatt gtttttcgat aaaaaatagt tgttttacaa taattatcct gctatatttt
      901 agctatcaaa gataaaagag ggataaaaat gaaatttaaa acattgctat taaaatttgt
      961 cgttttaggt ataagtgccg tatttatttt gttcggcgta cttttttata ctcagatcgc
     1021 tacaagcgaa aaaggattca attttgattg gcagaccaca ttatttgttt ctgtcattta
     1081 ctttgccctt ctcctaggga tcgttattgc ttatttctta ttgaaactat tgaaaaaaat
     1141 cgaagataac caagcttttt ccactcaaag cttaaacata ctaaaaaaaa tcgaacgaac
     1201 cattttgttt attggtttcg ccttttttgg cttacttcct aaatttatga aagtagcgga
     1261 tttagacgat gctccaggtt tgatgttggt aggaatcgct gtcgtattca ttccttttgc
     1321 agtatacaca ttaacaagcg tgctggaaaa actgcttgaa catgcgattt tacttaagac
     1381 agatcatgat ttgacagtgt gaggtgcgac atggcaatta tcgttaattt agatgtagta
     1441 ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga aagtgggcat tacgatggca
     1501 aacatctcta ttctaaaaaa tggaaaagca aaagccatcc gtttttccac tctcgaaaaa
     1561 atctgccaag ttttggagtg tcaaccaggt gatttgttga cctataaaaa tgactcgaat
     1621 actataccag aagaataaaa taaagtagaa caaacagtaa ataaaaaata atctgaatga
     1681 tagatgtgtg agacttttag aagttattta cgtctattat tcagattttt tatttcgatt
     1741 gaataccgtt ttatctataa ataagatatg tactgactca tacaaagggg gccttttctt
     1801 ctccatgtac tatcactttt cctgatacaa ctggtttcct gttacgttgt agttggatct
     1861 gcttttagta taatacttta ttcattccac cagactgata gcagtttgag cagtctaaat
     1921 aatcatgtag tattacaggc cattctgttt cataccttga agtgcttaaa acttcagcta
     1981 atggcccatt gtgttaactc ttactccatt cttttttagt aagctcccat atatactgta
     2041 aagccttcta tatattatgt catgttgctt tttaacattt actatgtagt tttgataaaa
     2101 tgatttgaat
//
LOCUS       DACCYH010000174         2065 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.184, whole genome shotgun sequence.
ACCESSION   DACCYH010000174 DACCYH010000000
VERSION     DACCYH010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.184"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..253
                     /locus_tag="IVY85_002908"
     CDS             <1..253
                     /locus_tag="IVY85_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8045856.1"
                     /translation="TEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNG
                     VTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            308..1873
                     /gene="guaA"
                     /locus_tag="IVY85_002909"
     CDS             308..1873
                     /gene="guaA"
                     /locus_tag="IVY85_002909"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP8045857.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
BASE COUNT          634 a          432 c          429 g          570 t
ORIGIN      
        1 tacggaaggc tttcaagaaa cccttaaaac aatttcatct acgttacctc agtcgtttaa
       61 aaagaaattc acgatcttcc atcgctacca atcgggtatt tcaaatgcct ttaaggtgtc
      121 ccactcaaac ggcgtcactg aggggctgaa taacaagatc aagctgatta aacgaattgc
      181 gtttggttat cgcaactttt ataactttcg ggcacggatc tatttacaac aaggtctgat
      241 ctttgaaaac taaaaaaata tgcggcgcac agaacgcgcc gcatatcgga ctaagtttta
      301 ttcaaatttg tcttcaccaa cacgatttga cgaagagcca aaaattatcg tacttgatta
      361 cggtagtcag tataaccaac tgatcactcg ccgtattcgt gagttcggtg ttttctcaga
      421 gttgatgagc caccgcatta ccgcagaaga agtaaaggca atgaacccta aaggaatcat
      481 cctttctggc ggaccaaaca gtgtttatga tgagaaggcc tttggtattg atgaaaagat
      541 ttttgatttg gggatcccag ttttaggaat ctgttacggt atgcagttga ttacttataa
      601 attaggcgga aaagttaaac cagccaaaaa ccgtgaatac ggaaaagcag aattggaaat
      661 cacttctgca gaagcggccc ttttccaaaa cactcctgaa aaacaaaccg tttggatgag
      721 tcatggtgac ctagttaccc aagcaccgac tgatttcgat gtcgttgcta ccagcaaaga
      781 ttgccccatc gcatcgatcc aaaatgcaga acgcaaaatg tacggaatcc aattccatgc
      841 agaagttcgc cattccgaat atggaaatga cttgttgcgc cattttacac tagatgtctg
      901 ccaatgtact ggtgactgga caatggataa cttcatcgac atggagatcg caaaaattcg
      961 tgaacaagtc ggtgacaaaa aagttttatt aggcctttca ggcggagtag attcctctgt
     1021 tgttggtgtt cttttacaaa aagcgatcgg cgatcaatta acatgtatct tcgttgatca
     1081 cgtattgttg cgtaaaggtg aagcagaaca agttatggaa agtcttggcg gaaaatttgg
     1141 cttgaacatt ataaaagtcg atgccaaaga acgtttctta tcaaaacttg ctggcgtgtc
     1201 tgatccagaa gaaaaacgta aaatcattgg aaatgaattt gtatatgtct tcgatgatga
     1261 agcaaccaaa cttgccggtg aagaaggcgt ttctttcctt gcacaaggaa ccctttatac
     1321 cgatgtgatc gaatcaggaa ctgaaactgc ccaaaccatc aaatcacatc acaatgtagg
     1381 tggattacct gaagacatgc aattcaagct gatcgagcca ttgaacacat tgttcaaaga
     1441 cgaagtacgt gaattaggta cccaattagg catgcctgac agcatcgttt ggcgccaacc
     1501 attccctgga ccaggcttag gtattcgtgt ccttggtgaa ttgacagaag aaaaattaga
     1561 aatcgtccgt gaatcagacg ctatcttgcg cgaagaaatc gccaatgccg gtttagatcg
     1621 cgacatctgg caatacttca ccgtcctacc aggcattcgc tctgttgggg ttatgggaga
     1681 tggccgtacg tatgactaca ctgtaggtat ccgtgctgtc acttctattg acggcatgac
     1741 cgctgacttt gcccgtatcc cttgggaagt cttgcaaaaa atcagtgtcc gtatcgtaaa
     1801 tgaagtcgca cacgttaacc gtatcgtgta tgatattacc agcaaaccac ctgcaacagt
     1861 ggagtgggaa taaagagcca acataatatg gatgaggaat acctataaat caacattatt
     1921 caattactaa agttcctgat tttcgtaaaa aataagatgg tttcctacca ttttccctac
     1981 caaagccctt gttagattaa ctactaacaa gggctttgct tttattcact acctttattt
     2041 tcctattgaa ttagcttttt atcta
//
LOCUS       DACCYH010000175         2030 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.185, whole genome shotgun sequence.
ACCESSION   DACCYH010000175 DACCYH010000000
VERSION     DACCYH010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.185"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            643..975
                     /locus_tag="IVY85_002910"
     CDS             643..975
                     /locus_tag="IVY85_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP8045858.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1594..1935
                     /locus_tag="IVY85_002911"
     CDS             1594..1935
                     /locus_tag="IVY85_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP8045859.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          705 a          271 c          428 g          626 t
ORIGIN      
        1 attaatttca aatatagaaa agggaacgta tgtttgcata ctcctttttt ttgagttata
       61 ataatacgcg aagagagata atttcattat ttaaacaagc taaaaatctt gatattactt
      121 cttttcaggc aatacgaata ttattcgctt acattttttg tataaattta attgtctgct
      181 atgcttgtga tgaattgatt ttcgttttgc acttttggat gataaatttg gacaattgaa
      241 aatacaaaaa aaagaaaggc ggtatgaact cggaaggctg gtaaccttcg tcagcgttca
      301 tacacggcat taagatttgg cccttaatgc gttaaccttt cagtgttacg gtattacccg
      361 caatggattt agtataacag atattcaaaa gtgataccac tttttttggc tgtgttaagg
      421 ttaaaaatac gtgatattac gatgaatcta tgcctaagca taatgttata aaaatgtccg
      481 tgtggctctt tgccgtatga cgaacgctga aattagaggg aacgcatgtt tggatgcgtt
      541 ccctcttttt tgtttgtcat gaacgaaaat caattctcta tttttgaaaa ataacaagaa
      601 aaacttgtta aaaatattta aagaaattgg gtggtttatt ttatgtcata tacaggaatt
      661 ttgagtctca aagatatttg tcactacggt aaacggtgta ccgctacaga gaaaatcacg
      721 aagaaactat caactggcca aagtaaaacc gttgtgcagt gtaagaaata tataatccag
      781 aaagacaaag tctcagagga aatgatttat tacattggga aacaaaagca aataatttta
      841 aaagatccta tccccttaaa agagctatat ccaacaatca aacatgttta tgaccaaaac
      901 ggtgtactga ttggtcgaag aaaaaatgga gtcttgcgtt gtaccgcaaa agggatgggg
      961 cgtttgatcg gctgaattga tcattctcat aacttgtatt ttatcgtttt tcgcttgctt
     1021 gaaaatattg tcgagacttc taaaggaact tagagtcact agaaaaataa ggagtagact
     1081 tgatctatca acaattccct tcggggagtg acggggtagg ttttaacgat tggtaacgta
     1141 aaaaattggt caatcgagta aatgcgcttg tttggatagt ggtgaaatga atttgtgtaa
     1201 ataataagcg cagagtaaac aagagggaat ttagaagtta gacaagttag aatagcgtct
     1261 agattaatca tgagcagcta ctaagggatc tagactttga cgcaaactca tggaataagg
     1321 agtaggtctt ctcgtaatgg ggaggacgca caagaaattg tgtcgtgtgc tgcagtacat
     1381 gtagatactt gcgaggacgt tgattattgg atcaatcgag catttaccgc tgtctactag
     1441 ggtttctcgt ttcggacgcc ataaacgagt cgaaattttt tatgtatttt taagtaaacg
     1501 aaaaacgata aaatatgaac aagtcctgaa taaagtgaaa aaattgatat aaaacataga
     1561 gaaaatgaaa tttacgtaat ggaggaatga caaatgaaaa attatgatcc gaatatacga
     1621 ttgggcacac atactattaa ggtttctttc caacgttggg attacaaagg atttgtgacg
     1681 tttagaaggg gcggaaattg taaaggatta gatgttttag cattagacga ggatgattta
     1741 tacgatcaaa cattgactga taatccaatt ggattcggct tgctacctga agatgatgaa
     1801 ggtaatgaat ggtttaaaat gacgcttatg aatgataatg gagacgaatt atctgttgaa
     1861 gatacttggt catatttgag tgattatatt gtcagctttg aaatcattga atttgtagcg
     1921 gataaagaag aataaaaact ggaggaataa attttacaga taaaggctta gaaggagtca
     1981 cgacgattaa taaaaaggta gtattacctt aggcattgag taaggaattt
//
LOCUS       DACCYH010000176         1971 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.186, whole genome shotgun sequence.
ACCESSION   DACCYH010000176 DACCYH010000000
VERSION     DACCYH010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.186"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            89..571
                     /locus_tag="IVY85_002912"
     CDS             89..571
                     /locus_tag="IVY85_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045860.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            768..1658
                     /gene="pstS"
                     /locus_tag="IVY85_002913"
     CDS             768..1658
                     /gene="pstS"
                     /locus_tag="IVY85_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP8045861.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          644 a          337 c          407 g          583 t
ORIGIN      
        1 tctctaaaaa aaaagttttt ccccctaatt tttttagata gaaaattgta tagtatgatg
       61 agagaaaaag tagtttgggg gaacgttaat gagaagaaaa gcagtaatcg atcggcttta
      121 tttatttttt tggttttttc ctatcgtttt gctacctttt acatgggtaa atgctgttgt
      181 acaaggtgcg gagatattag gcctgatcca aggaagcgga tttgtggttt ctaggaattt
      241 tccagctttg attgttttct atttagtgat aagtgtaggt ctcttattac caaaatctct
      301 gatcaatcac tacaattggc agctgcttta cttttcttgc ttattagtat ttacatctac
      361 gttttctttg ctgcctcgct tgattatcgg ccctaatggt ttgagcgaga gtggattgaa
      421 ggatttagct tattcctcca tgtatttctt gacgatcaaa ccatggttct atttagtaat
      481 tgcctcattt attcttgcaa cattttttta cttctggaca gaaatcagga taaaaagtga
      541 acgatctgta tctataaaaa ataactattg atggtggatc atcagtaaaa aaacatatga
      601 gctgcgtaaa atgcatgttt ctcatatgct ttttttatta ttatgtaaag aatgcgaaaa
      661 acgtttacat gatttttaca taacttaact atatttttac agcaaccata cgagggattt
      721 acagtcgact gataaacttt agttgtaaat aagaaaggag caataaaatg aaaaaagtat
      781 tgtttactct aggtatgatg ggaattcttt tagccggatg cggctcagga tcaggaaata
      841 cagatggctc tgccacacaa aataattcaa gtgactcaaa tgaacaagta aaaatcgttg
      901 cggtaggttc gacagcatta cagccgttag tcgatgcagc acaagaaagc tttgtacaag
      961 aaaatccaaa ttatcaaatc tctgtacaag gaggaggaag cggaactggt ttgagccaag
     1021 tcgaagctgg agcagttact atcggaaact ctgatgtatt tgcagaagaa agagacggag
     1081 ttgatgcttc aaaattagtg gatcataaag tagcagtggt cgggatggca ccaatcgtca
     1141 ataaagatac agatgtcaaa gatatcacca aacaggaatt gattgatatc tttacaggaa
     1201 aaatcactaa ctggaaggaa gttggcggga aagatcaaaa aatcaacgtc gtgaaccgtg
     1261 caaacggcag cggtacccgc gcgacattcg aaaaatgggg actggatggt gctacccctg
     1321 tgcagtccca agaacaagat tcatcaggaa cagttcggca gctggtaagt caaacaccag
     1381 gagcaatcag ctatttagct ttctcatatc tcgatgattc cacacaagca ctaagtatcg
     1441 acggtgtaga accaaaagaa gagaatgtag cagataacag ctggggaatc tggtcatatg
     1501 aacatatgta cacgaacgga aaaccttccc ctgaagtcca aaaattctta gactacatga
     1561 tgacagaaga aatccaagaa ggacctgtca aagaattggg ctatttacca atcacaatga
     1621 tggaagtaga acgtgatcac gaaggaaata tcaaataact agcagataaa catgccacgg
     1681 tagtcgttac tattaaggaa aaatcaacta tgtttcccca catatacatt tttcctttaa
     1741 ttgtgaaaaa tagttcctca tattttgccg ttttttgagc taataccgag ggtcagctga
     1801 ttgaacaccg tttattcgaa aaaaagagct acgacataga actgtcgtaa ctttttttgg
     1861 ctcttcgtca aatcgtgttg gtgaagacaa atttgaataa aacttagacc gatatgcggc
     1921 gcgttctgtg cgccgcatat ttttttagtt ttcaaagatc agaccttgtt g
//
LOCUS       DACCYH010000177         1955 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.187, whole genome shotgun sequence.
ACCESSION   DACCYH010000177 DACCYH010000000
VERSION     DACCYH010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.187"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(126..1127)
                     /locus_tag="IVY85_002914"
     CDS             complement(126..1127)
                     /locus_tag="IVY85_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP8045862.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(1286..1552)
                     /locus_tag="IVY85_002915"
     CDS             complement(1286..1552)
                     /locus_tag="IVY85_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8045863.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1542..1898)
                     /locus_tag="IVY85_002916"
     CDS             complement(1542..1898)
                     /locus_tag="IVY85_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8045864.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          581 a          369 c          234 g          771 t
ORIGIN      
        1 tggtcccccg catttacgat tgcacaagag aaacataaag tcgacctatt agcatttctt
       61 ttcaattcaa acattaaaaa agtttatacc tctgctacta aaaaatgaaa ttatctattt
      121 aaaatttaaa acggtaaatc tgattcatcc aaattggaaa atggatcagc ctgtattgga
      181 gctttgtatt tctccataat aacagatatc tttttttcat tttttaaaaa atacctttta
      241 aatttcttat agtcctctga aatcaaaata tagcgtgatg gatactcatc aaaagaagaa
      301 tcgtattctt cctttaaaat tcccgattta cttaattttt ctagtacatc aaatactcta
      361 tcactcaagt aactccttaa attgttatca ctttcccatt gaactacttt tctataagtt
      421 gaattatcag ttgaaaagat agaattttca tccataaacg catatattag cagcaatctt
      481 tcatcattgt caaattctat tggatcaaaa atatctaagt tgtcatcaaa atctttatca
      541 gttccggtaa cttttttgtt aattactgaa ggtgtcttgt aagaaataga atctaaatca
      601 aagcgttttg attctaaatt attttgtgct ttctcaatta aataattcac tttttttgtg
      661 agaaaccttc tgatattgtc tttgctatat gttccataaa aacccttatc ttctaaagag
      721 tccctgtact tcttaacttc tatatactct tcagccaaaa cttgtggaac tggttcatct
      781 aagaaaaaaa cccctacctc gcctttgcta tttttataaa agtaatcaat ttctgctata
      841 gttcccgatc tataattatc aactggagaa cccaacttat ggccaaaaat cgctaataat
      901 atatcactat tttctacaat ttgtttatta ataacacttt gcccagagct attatttata
      961 taagtaggaa tcgcattcgt ttcccatcga attggcatca aaattacttt tgtattatct
     1021 gtattttcta tattccatct atatatggtt tcttctactt cattcctctt tgctgctata
     1081 tcacctggtg atgctataaa tacttttatt agatttatgg ttttcattta ttgtaccttt
     1141 ctctttatgt ctctttaaag taatttttgt ataaaattta ttgactttta cactatagga
     1201 caacttttat ctatttagta caaaaaggcc cactaaacta attgattagt taggagccta
     1261 ttacaggtat attttcaact cgcttttaag caaattctac taccagcttt ttgcctaatg
     1321 ccctagcgat atcatcaagt ttatcgaaag atacgttatc ccctctctca attcgagcaa
     1381 tagtagattg agttgtatgt gctttttgtg caagctccgc ttgagttagg cctgcttcct
     1441 cccgtgcatg ataaagagca accgcagttt cgagttttct tccttcacgg tcatatgctt
     1501 ctgcaaaatc tttgtctttt tgcttttctt caatcaattt tttaatcatc atttgtgaac
     1561 cactcctctc taagttcttt tgcatgttta agttcactta ttggtgtttt ctccgttttt
     1621 ttagtaaatc catgggtaat gacatacctt ccattgacca catggaaata gatagctctt
     1681 tggatgtttg atcctacctt acttcttaat tcgtacaaat tagtgtctaa ttttttcacc
     1741 catttcaacc gttgagctac tagcatccca ttcttttctg tttcttctat tgtacgtaaa
     1801 agcttcgcac tatctttttt tggtaatgtt tccaaccatt ctaaaaattc atcatgacca
     1861 ttaggtcttt taaatgtttc aaaagatatt ttttccatac ccaattatat catatattga
     1921 ttttatatta tagtatataa cataaaaatt cgaat
//
LOCUS       DACCYH010000178         1945 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.188, whole genome shotgun sequence.
ACCESSION   DACCYH010000178 DACCYH010000000
VERSION     DACCYH010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.188"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            93..1559
                     /locus_tag="IVY85_002917"
     CDS             93..1559
                     /locus_tag="IVY85_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8045865.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT          601 a          334 c          397 g          613 t
ORIGIN      
        1 acacacataa aataacactg tcttgtaagt ttgttaaaaa ttgaatatga ttacatctgg
       61 ataacaaatt tttatcataa aggagagaac gaatgaacaa caaacaatta cgctggttta
      121 ctgttgcgtt aattgcattt aacatggttt ggggcatggg gaatgtagtt aataactatg
      181 ctcagcaagg agtatcagtt gtcacctctt ggctattgat tttggccatt tattttattc
      241 catacgcatt gatcgttgga caattaggat cagccttcaa ggatagtaaa ggaggcgtca
      301 gttcatgggt cgaaaatacg acatctaata aacgactggc atactatgcc gcttggactt
      361 actgggtcgt ccatattcct tatttggcac aaaagcctca agcaattctg attgctggtg
      421 gttgggcagt ccaaggaaat ggaaatatcg taaatacgat gtcagtacaa atggtagcag
      481 ttatttcact tattatattt cttgcctttt tatatttatc aacaaaagga ctttctactt
      541 tgaaagtgat tggcggactg gccggaacag cgatgttcgt catgtcgatc ttatttattc
      601 ttttagctgt aacggcaccg ttcatcaatc caacgatgga atttgccaca ccacatatga
      661 atcaagtgaa aacatatatt ccaacatttg atttcagtta tttcacaacc gtttcgatgc
      721 tagtatttgc agttggaggg gcggaaaaaa tatcccctta tgtcaactct acaagaaatc
      781 ctgcaaagga atttcctaaa gggatgatct tcttagcagc aatggtaggg atttgtgctg
      841 tattaggctc agtcgcaatg ggtatgctgt ttgctagtga taatattcct aaagacttga
      901 tggcaaatgg ggcatacagt gcttttcaaa ttttaggtaa ttactatggt atcggtaact
      961 cattgatgat catttacgca ttgacaaaca tgattggaca aatctccgca ttagcctttt
     1021 caattgatgc accactaaaa atcttgttag cagatgcgga tccagaattt gtaccaaact
     1081 ggctacgtaa acgtaatcgt aaaggtacat tggtgaatgg ttatcttctg acaggtatac
     1141 tagtaggaat cattattttg ttgccgattt ttggtatcaa agatatgaat gaattagtaa
     1201 aatggttgac gaacttgaac tcagttgtca tgccaatgcg ttacctatgg gtgttcttgg
     1261 catatatgat gttgaataaa gcttataaga aattcaattc tgaatataaa tttgttaaaa
     1321 atccaaaaat cggttttatc tttggtgctt ggtgctttgc cttcactgcg tttgcatgta
     1381 tcctaggaat ggtgccaaaa gtagattata ctgctgatcc taaagcatgg tggttccaat
     1441 tatttacgaa tatcatcaca ccaatcgtct tgatcttatt aggtatgatc ttgccagcaa
     1501 ttgctcgtcg agacaaaaac aaagaaatca ctgaaccaga aacagtggtt cagccttaaa
     1561 atagaaatac ttgttacaag aggttgtgac agaagtggtc agcttcaaga aataagaagg
     1621 aatttacgaa aattgcttct caaatttttg tgaagtttcg gcttgttttt cgaagaagct
     1681 acttctggaa caccgtgaat cagaggttgt gaaaaagctg tcctgcatca agtaataaga
     1741 acagccaaac aaaaatagcc cctcatattt ttcgtttggc tgggcttatt accgcagatg
     1801 caagctttgg aacaccgttt aaggaagagg ttgtaaaatc agcgatttga cctgagtatt
     1861 agagaaacaa acggaaaaat agctcctcat attttgccgc tttttgagct aatgctgaag
     1921 gtcagctgat tgaacaccat ttatt
//
LOCUS       DACCYH010000179         1881 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.189, whole genome shotgun sequence.
ACCESSION   DACCYH010000179 DACCYH010000000
VERSION     DACCYH010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.189"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            42..1469
                     /locus_tag="IVY85_002918"
     CDS             42..1469
                     /locus_tag="IVY85_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP8045866.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            1471..>1881
                     /locus_tag="IVY85_002919"
     CDS             1471..>1881
                     /locus_tag="IVY85_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045867.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSK"
BASE COUNT          627 a          213 c          311 g          730 t
ORIGIN      
        1 aaatagaatt gataaagaaa ttttgaaagt tggaaatata gatgaataga atagtacctg
       61 ttttagagaa gagtttatta agtcgagatg cgtttcgagt aataaattta atcatttgtc
      121 tgctttattt tatgcctagt acttttaatg taactaatat cccgatgaaa atagcttttg
      181 cttggggtgg aattatagtt ttgtatgagg tttttataaa gaaaaatttt tttaaaatgc
      241 gtttttcttg gctactgatt ggagcaatga ttagttttat cttaactata atcattaaca
      301 gagaaaattt tttaattcca tctatttata attttggata tttgctgatt actttgatag
      361 taatttttcc tgcagacttt tcacaaagtg aagaggaaaa aaagaaaaaa atggtgtatt
      421 ttaataatgt tttcattatc ataatttttt tagctgcgct aatatctatc tatcaattta
      481 tttttttgat ttcttatcat gtcccaacag gaacgccagg attacttgca agacaaggtt
      541 ttattgaaca tcggttattt ggtgtctata caagtccaaa tgttggagct atgtttggat
      601 atacttctat tattttaagt ttaattagtg tattaatttc aaaaataaca aaaacgattc
      661 gtgtttttta tatgatgaat tttatcgtac aaatatttta tattacactt tcttcgtcta
      721 gaggtgccca ggtagtaatt tgttcttttt tagtattatt tcttttgttg tttgccaatt
      781 caaaatttag aaagagcatt agagaagtac tacctgtaaa aatcaaatgg atggtaagca
      841 tattattagt tctattggta tatttttctt ttggtactaa ttatgctaag gattttttag
      901 caacaattcc tgtatcgatt gatagaaaag tagaagttat agagtcaaat gaatcagata
      961 aaactccact tccagaaaaa gaagtaatat tacagcattc tagtgataac tccgaaattt
     1021 cagctggtcg ttttacgatt tggaaagctg cgcttaaagt tataagacca actttgttat
     1081 ttggttatgg agagaccgat ttctatggga atagcgatgc aaaatttttg aaaaaagtag
     1141 atcttgatac tacagatgtg aatgaactaa aaagagcaca tggaaacatg cataatggat
     1201 ttcttcaagt acttgtatca agtggtataa ttgcttttat atgtatctat gctttctatg
     1261 tgttaaatat ctttagttta ctcaaaacat tttttagaag taaagtgaat tatgcgtttc
     1321 ttggtattgt tttaattttt atactatcta tttttataga tgaaatggtg gaggctcatg
     1381 tacttttcaa taagcgcgat gtcatttcaa ttgttttctg gtattatttg ggaagcattt
     1441 cttacttgta tttaaaaaag gagaattaat atgaagacag ctttcattgc ttggactcct
     1501 ttacatttga ttaatatatt aaatgtttat caaaattttt accgagaatc aaaaggagat
     1561 ttatttattt attctgaatt tgaaaacgct agaagatata gtgagcgtat aaaagaatta
     1621 gggctattta atgatgttgt ttttgtgaat caccggaaaa tcggttcttc ctttaaacgt
     1681 aaaattaact tgttaactaa ccataatcaa tttttctcgg atgatttatc cgtttatgat
     1741 gaaattttta tacaggggga aaattatttc acaaaaatac ttttctccaa tgtttacaaa
     1801 aaaaatccag atgtaaaact aaattatata gaagatggtt tgggtactta tttgaagaag
     1861 agtatatttt caacaagtaa a
//
LOCUS       DACCYH010000180         1881 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.190, whole genome shotgun sequence.
ACCESSION   DACCYH010000180 DACCYH010000000
VERSION     DACCYH010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.190"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(325..780)
                     /gene="tnpA"
                     /locus_tag="IVY85_002920"
     CDS             complement(325..780)
                     /gene="tnpA"
                     /locus_tag="IVY85_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP8045868.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1023..1881
                     /locus_tag="IVY85_002921"
                     /pseudo
     CDS             1023..1881
                     /locus_tag="IVY85_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          597 a          365 c          354 g          565 t
ORIGIN      
        1 caaaaatact tccaaaggtg ttcgataatc cagtgatttt ctaggaagat tatttctgcg
       61 ccagcatttt ctaatgtttg aatttgtgtt ttgtaatctt ggttgcttgt gctaattctt
      121 gaatagccgt aaattgccaa aatttcatca ccctttctgg acataagttt ctgacaccag
      181 ttaaaatcaa gtctatcagt aacccataca cccgtgagga cgggtgtatt tttaatcccc
      241 ctagaagggg tcgacactcg ctgcccctta aaaggggatt tttaaaagac cgccaactgc
      301 tcatcttcac tcacttactt ctctttattt tttaaatggt tctacgtact ctcttttact
      361 tataatatat cgaatgcgat catcagcttc ttgttcacgg atgtattttt catttgtctt
      421 ttgattcaat ccgactgtac tcacatagta tctcttcgac caaaaggatt tgtttccgta
      481 tttatatttg agatttgcgt gctgctcgtg gattagtacc gcactacgac ctttcagata
      541 ccccataaaa ctggatatac atatttttgg tggtactcga acaagcatgt gaatatgatc
      601 tagcattgca tgtgcttcta tgatttccac atctttcatt tcgcacaact tcctaagtat
      661 ttttccaaga tcttttcgta attgcccata gattactttt cgttgatatt ttggtgtaaa
      721 aataatatga tacttacaat tccaacgagt atatgctaaa cttttatcgt catttgacat
      781 gaaaaaactc ctttcgatct gatgagcggt tggtagccac tcactagtat atcgaaagga
      841 gtttttatcg ctaaagatac ttcttccacg cgtagaaacg cgcggttttc ttgtttcgca
      901 cgctttacgc acaaaactga ctgaagtcat taagaaaata aaagggtcta aaacttttaa
      961 gttttgtacc gacccccaaa agttagacca aaaaatctaa cttttggagg tcggtatttt
     1021 ttatggctaa gtatactttt gaacttaaat taaagatcgt tcatgattac ttggatggta
     1081 aaggcggaag tgattatctg gcaaagaaat attccattaa ggctccatca caagtgaagc
     1141 gctggatcaa tgcctatcaa gaatttggtg aagagggtct agtaagaaag cgacaaaaga
     1201 aaaaatattc tgttcaattt aagctggatg cgatagagtt atatctaaca agtgagctgt
     1261 cctatcgtga agttgccaac acacttaaca tgaacaaccc taatttgatc gctagttgga
     1321 tgcgtcaatt ccgtgagggt gggatcgatg gactctcaaa aacgaaagga tgtccaccta
     1381 tcttgtctaa gaaaaatgaa cccaaaaata agaaaaaatc tacaactaaa gcaacgtcta
     1441 aagaacgtga acgcatcgag gagttggaga aacgagtccg ttcccttcaa attgaaaatg
     1501 cctttttaaa agaattgagg aagctgcgaa aacaggaagc ccaacaacga cgaatgaagc
     1561 aatcgcacga atcatcgcaa gcctccgagg accgtttaaa ttagttgagc ttctagaaac
     1621 tttaaagttc cctaaggcaa tctttatgta ctggcaaaaa cgattggacc gtaaaaatcc
     1681 agatcaagag attgaagcgg aaatgcttaa agttcgggag aagcacaagg attatggctg
     1741 tttacggatg accaatgaac taagaaaccg cggtttctct atcaataaaa agaaagttca
     1801 gcgcctgatc aagaagctag gaataaaagt aacgacatac acacgtaaat cacggcgcta
     1861 caattcttat cgaggacaag t
//
LOCUS       DACCYH010000181         1859 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.191, whole genome shotgun sequence.
ACCESSION   DACCYH010000181 DACCYH010000000
VERSION     DACCYH010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.191"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="IVY85_002922"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="IVY85_002922"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8045869.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          654 a          343 c          444 g          418 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagtt
//
LOCUS       DACCYH010000182         1854 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.192, whole genome shotgun sequence.
ACCESSION   DACCYH010000182 DACCYH010000000
VERSION     DACCYH010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.192"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="IVY85_002923"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="IVY85_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP8045870.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1305..1709)
                     /locus_tag="IVY85_002924"
     CDS             complement(1305..1709)
                     /locus_tag="IVY85_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP8045871.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          551 a          329 c          299 g          675 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga
     1381 caaaaacttt tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca
     1441 cgttttatca atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt
     1501 ttaggttgag ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa
     1561 gtaatgtgat acgattctga aattctttca aaagtagttt tggcataatc agatatttca
     1621 tcatcaatca cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat
     1681 actgaatgat tatttgtatc taattccatg ttatatcaac tcatttcttg tatagactga
     1741 ttgtaacatg gaacaaaata aaaaggcaat acgccttagt tgtcggtctt cgagttacca
     1801 acggtaaccc tcataccgaa cggcattcat cacccaccta tagaggatgg gcga
//
LOCUS       DACCYH010000183         1847 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.193, whole genome shotgun sequence.
ACCESSION   DACCYH010000183 DACCYH010000000
VERSION     DACCYH010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.193"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            700..>1847
                     /gene="ltrA"
                     /locus_tag="IVY85_002925"
     CDS             700..>1847
                     /gene="ltrA"
                     /locus_tag="IVY85_002925"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004853597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8045872.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQK"
BASE COUNT          730 a          281 c          384 g          452 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaaga
//
LOCUS       DACCYH010000184         1841 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.194, whole genome shotgun sequence.
ACCESSION   DACCYH010000184 DACCYH010000000
VERSION     DACCYH010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.194"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..613
                     /locus_tag="IVY85_002926"
     CDS             <1..613
                     /locus_tag="IVY85_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045873.1"
                     /translation="KKIDDYFVYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIE
                     EVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKN
                     ILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFEKTINSIMLAKLILNEYTALS
                     EDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLVEYGII"
     gene            600..>1841
                     /locus_tag="IVY85_002927"
     CDS             600..>1841
                     /locus_tag="IVY85_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP8045874.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQ"
BASE COUNT          615 a          236 c          288 g          702 t
ORIGIN      
        1 aaaaaaaatt gacgattatt ttgtctattg tccggaattg ttactttatg atgtaaaaaa
       61 tataaaaaaa attcccaaaa tagatgaaaa cagtaaattg atgaaaaaca tacaatcaat
      121 ctttgggata gaagaagttt ttgtcggaga taaatgtatc ttttttgatc aacctttatt
      181 atctgatggt tatggcatag atgaaacaga tattttcgct aaagtaaata gtatattttc
      241 tggaaaaaaa gaaactttaa ttaagcttca tcctagagca aggacaaatc gttatggtga
      301 aaaaaacata ttgaattcca gtttaccgtt tgaagtcttg ctacttgaaa atgacttctc
      361 ttcaacactt ttgattagtc ctatatctac aatttctttt tcaccatcta tgatgtttga
      421 aaagacgatc aactctatta tgttggctaa attaatttta aatgaatata cagcattatc
      481 tgaagataaa aaagaaacat tgacaaatat tgttcttttc tgtgaacatt ataaccgtct
      541 atcagaagaa aaaattttac tcccagaaaa ctgggcagaa ttaagaaagt tggtagagta
      601 tgggattatt taacacactt tccaagtcaa aattagttaa gtccagtttc tggtatacta
      661 tagggaattt ttttattaag ggaatttcat tcataactat tcctattttt gtaaatatta
      721 ctactcaagc agaatatggt ttgattaata atttcacttc atttgtatca attttttcga
      781 ttcttgttgg gctaagccta aattcggcaa ttaataatgc aagttttgac tataaagatg
      841 acataaatga atttatgtca tcggtacttt ctctagctac actttcgtta attgtgttct
      901 tattaatagg gaacatatat tttttgtttt caaatgcttt ttttgatatg aaccaacttg
      961 tatttaattt gatgttatta caaagttttg ctagttttgt aattagttat atccagtctt
     1021 attttacgat taactttaac cattttaaat atttgatttt atcttttata tctacttttt
     1081 tgaatattgc aatctctgta gctttgatgc agacattata ttttgatgat aaatatgttg
     1141 gcagagtagt cggcggaaca ctgtcatttg tgttgatagg tttagtaatt tatttaagaa
     1201 taatgataaa gggtgctcat ttttttgata aaaaacagtg gagatacgct ttaaaaatct
     1261 ctttgccgct aatcccacat tccttagcaa atatagtttt agcacaattc gatagaatta
     1321 tgattaattc ttatttaggt cctgctgcgg ggggaatata ttcctacatt agtaacatag
     1381 gtattattct aagcgtaata tggatgtcta ctaataacgc ctgggttcca tggttctatg
     1441 gtgagatgag taaaaataat gaaaaaaaag tgaagaaagt tagtaactat tatttagtag
     1501 tctttacttt gattacgatg attgtaatgg tagtttcgat tgatttagca aaatttatgg
     1561 ctccgaaaga ctattattct gggttatctt tagtggttcc gataacctta ggctatttct
     1621 ttcagttttt atatagttta ccagttaatg ctgaatttta tgagaaaaaa acatcgttta
     1681 ttgcgattgg tacagtagca agtgctatag ttaatatact attaaacatg attttcatac
     1741 ctaggttagg aataattgct gctggataca caaccgtatt tacgtatttt ttacttttta
     1801 tttttcatta taatatcgct aaaaaaatta cgaataaaca a
//
LOCUS       DACCYH010000185         1789 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.195, whole genome shotgun sequence.
ACCESSION   DACCYH010000185 DACCYH010000000
VERSION     DACCYH010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.195"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            57..197
                     /locus_tag="IVY85_002928"
     CDS             57..197
                     /locus_tag="IVY85_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8045875.1"
                     /translation="MSDSIEKMTLKKLADELGVSKNTVRNQLVNSGFDLAQYKEKNAV
                     VN"
     gene            complement(534..950)
                     /locus_tag="IVY85_002929"
     CDS             complement(534..950)
                     /locus_tag="IVY85_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045876.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKI
                     EKQLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
     gene            complement(952..1233)
                     /locus_tag="IVY85_002930"
     CDS             complement(952..1233)
                     /locus_tag="IVY85_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045877.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIEND
                     FDHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
BASE COUNT          517 a          256 c          354 g          662 t
ORIGIN      
        1 aaacgagaat tagatacatg gcttcaagga cttgatgtga cggaagtagg tgctgaatga
       61 gtgattcgat agaaaaaatg accctaaaaa aacttgctga tgaattgggt gtatcgaaaa
      121 atacggtgcg caatcaactg gtcaattcgg gatttgacct tgctcagtat aaagaaaaaa
      181 atgcagtggt caattgaccg aaatttatgt ctttgggttt tggggtttta aaacgaaaaa
      241 aagggggcga ttttcgcccc ctttgctcta tgtgaaaaat ttgttatact tattttatcc
      301 ttaaaacata ccaatctttt tcttatttag ctgctcgtac gagcagcttt ttttacgatt
      361 atgaattcaa atcatgtatt tataattttt ttgtttttat ctttttttct gttatttctt
      421 ctgaaattcg tttttttcat tcaattggtt actatcttat tgtaaatgtt gctattaaga
      481 tggtgatcct acccaaaaaa taaaccagga atttaaatct cctggtttat tttttatctt
      541 tttagaaaat ttgttgggtc gaaattttta gaagtaaatg tttgattggt ttctttttga
      601 cgattttttg gttgatttcg tttggaggga ttaagttgtt tttcaatttt ttctttcgtt
      661 tcttgataca ctttagaatt tgtgccaata agaatacctg tatcttttag atataaatca
      721 gcaaattctg caatgatttc ggacatgatg gaaatttctt ttcccatagc atttagtttg
      781 gcttggttgc tggtagtcgt agtattggta tcaggttcat aactcataca taaaaaacga
      841 atggcttccg agtaattgtt gatatctgga tttctttcga taatggaatc taaaacgtcc
      901 acgtgactgg gataaagggt aacgtgtttt tgaacagaag tttttctcat gttatcttcc
      961 tccgataagt gtttcaatga gttggttgtt tgtacgaaca tattcattca gcgtttcagt
     1021 atttttttct tgaatagcga taaatttttc gagtaataaa tcgaattgat ctttcttgta
     1081 atgcataagt gcgtggtcaa aatcgttttc gattaggact tttaagtagc gatttcgact
     1141 ccattttttg ccagttttat ttgtgagttc ttggcaacgt ttgtctattt ttgcaataac
     1201 ctttgaatcg atcttccgaa ctaaaatgtc catagaaaaa ctcctttcgc atttgtgtat
     1261 aatttatgta tatcatacga atatagtatt tgtatagctg ttttttgcta aaatactata
     1321 cataagctag ccacgatggc gaaaaagtga tatcaaattt ttttctgaga aacgtgatag
     1381 gcacagccta tctacccact ttttttagaa agtgggtaca cgtttcctta tgctctttat
     1441 aaaatggtga tgtgcgactt ttttctgttc gtaaaaataa aaaaagttta cgaaaatgaa
     1501 actaaactga acgattatga cgggcatgta atcaagatga aatgatttta taagcgcgta
     1561 ttttgcgttc taagacgttt tagcgttcat ttcgtttagt tatcggcaca atcgttaaaa
     1621 cacgtaagag aagcgagaat ggagcctgta aggcgactgg tagcgaaacg gcgtgttaga
     1681 ttgtgcaagc cgagaactga atgaaatgaa gagcggaagc gtgaaataat ttcggcagga
     1741 ggaggctcaa ggggttcggg gaatgaaatt ccctgaggtc tttttattt
//
LOCUS       DACCYH010000186         1773 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.196, whole genome shotgun sequence.
ACCESSION   DACCYH010000186 DACCYH010000000
VERSION     DACCYH010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.196"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            81..1604
                     /locus_tag="IVY85_002931"
     CDS             81..1604
                     /locus_tag="IVY85_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP8045878.1"
                     /translation="MDPTVFEAEEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNRLARKRAEFKQFGKQIDLVIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPEKTKGLIVTFRKPATMEKN
                     VEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEEQIGVYETVKGSLMEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHAFSNEWFVSEKELHASNLQYM
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          641 a          285 c          359 g          488 t
ORIGIN      
        1 tagaaacata tgcagagtat tcaccggaac agatcaatcg tatgatcgat caaatggagt
       61 ctgtcaaaaa agaaagttat ttggacccta ctgtttttga agcagaggaa catatccaaa
      121 aagtcattca taaaatgttc cgtccagaca atgcctatac aaaatttgat tttcgaaatg
      181 gccgtccaac gaagtctgcg atcttaacca cgagttccat tgatatggcc aaaaagtatt
      241 atcgggccat caaagagatg acgaaagaat ctgattggtt aataaaagag tttcccaatc
      301 agcctatccg tgaaggacga acgatggagg atcctgattt cccacgaatc gctattacct
      361 attcattaga agaaaatagc cgagattcaa gtgcccaaca agaagaaatg caaaaaatta
      421 ttgaggaata taatcagtat tatgatactg cctggtcact agcagacatt gagcgttata
      481 acggagacat caataatcgt ttggcacgaa aaagagcgga attcaaacaa tttgggaaac
      541 aaatcgattt agtaatcgta gtggatcgtc tattgacagg ttttgacgcc cctacgattc
      601 aaaccctatt tgtggatcgt aatctagagt atgcaggatt gatccaagcc ttttcacgta
      661 cgaaccgtac gtatcctgaa aaaacaaaag gactaattgt tactttccga aagccagcga
      721 caatggagaa gaatgtcgag gatgcgacaa aactgtattc agaagctaaa gaagaatcgg
      781 gtttgattta tccaagttac caagaatcca aaaaaagatt caagcaagca tatggtaagt
      841 taaaagaggt ttcatccatg gaatccattg atgaacatac gccacttgaa atccgtatcg
      901 agtacgtcaa agcatttcaa gaattgaatc gtgcgtatga atcgttagtg acttatgatg
      961 attacaatga tgacatggtg caatcttcaa ctttgaacaa tcaaattcaa cttttggaag
     1021 aacaaattgg cgtgtacgag accgtgaagg gttcattgat ggaggaagaa ccagaaaaag
     1081 agggagaaga tttttcaacg atcgaatttt atagtgaaaa taccactaag ttgtatgata
     1141 ttgattctac ttatattgat caattactag gaacttatgc tgcgaatagt tcagatgcac
     1201 gagaagaaat tgaaaaagct ttggctaaat tgaataaaac agaaggagtc aaagaagttt
     1261 accgacaaat tttaaatgca tttgatgtgg ggactttaga tagtaatgaa gatatctttg
     1321 tcattaaacg tcgctatttt acacaagcaa gtgataattt gattcatgcc ttttccaatg
     1381 agtggtttgt ttctgagaaa gaattacatg cttctaatct gcaatatatg ccaggtgaag
     1441 atccaattcc taatatgaaa gcaattatca acagtaagga ttacgaagga tataaagcca
     1501 aacatccaga agcgaaacca tttaaatacc cacaagaaat gaagcgagca tggcgtaaaa
     1561 tgttagatga tgagttgatt ccacttgaaa atgaattgag gtaaacgaga agagttaaga
     1621 aaattgtacg attcaaagat agtagtaaag tggaagtgta gaaaataggg atagatgtct
     1681 ttttacttgt tcacatttta taacggagta tttaagaaag ctattgaata atgattcttc
     1741 catctttttc attcatttac cggttctgtt gca
//
LOCUS       DACCYH010000187         1664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.197, whole genome shotgun sequence.
ACCESSION   DACCYH010000187 DACCYH010000000
VERSION     DACCYH010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.197"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            131..505
                     /locus_tag="IVY85_002932"
     CDS             131..505
                     /locus_tag="IVY85_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8045879.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IVY85_002933"
     CDS             complement(619..1248)
                     /locus_tag="IVY85_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8045880.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IVY85_002934"
     tRNA            complement(1382..1454)
                     /locus_tag="IVY85_002934"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DACCYH010000188         1611 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.198, whole genome shotgun sequence.
ACCESSION   DACCYH010000188 DACCYH010000000
VERSION     DACCYH010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.198"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..626)
                     /locus_tag="IVY85_002935"
     CDS             complement(<1..626)
                     /locus_tag="IVY85_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8045881.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
     gene            914..1567
                     /locus_tag="IVY85_002936"
     CDS             914..1567
                     /locus_tag="IVY85_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8045882.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          578 a          226 c          299 g          508 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gatgggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcta tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtctgccca
      361 caatcatagc atagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      421 ttcaatatct gagtacgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      481 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccatagag cgactccttt ggtagggtga tgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caacaacaca aaatattata gaacccttta ttctaaagga
      781 tttcaaggtg tctgtttgca tttattagaa aaaagaaaaa gtattggcaa cagaaggtgt
      841 caccaacact agatattata gagccataat atgctgtata atatctagtg tataggctta
      901 agtggaggta tatatgaaaa tattaactgt tgaagatgat aatatgataa gagagggaat
      961 aagtgaatat ctttcagaat ttggatatac tgttattcaa gctaaagatg gaagagaagc
     1021 cttgtcaaaa tttaatagcg atataaattt ggttatctta gacattcaga taccttttat
     1081 aaatggttta gaagtgttga aagaaattag aaagaaaagc aatttaccaa ttctaatctt
     1141 gactgcattt agtgatgaag aatataaaat tgatgcattt actaatttag tagacggata
     1201 tgtagaaaag ccattttcat tgccggtctt aaaagctaga atagattctt taattaagaa
     1261 gaattatgga catttagaga agtttgaata taaggatcta tcggttaatt ttaatagcta
     1321 tacagctaaa ataaatggtg aagaaataga tgtaaatgca aaagaactag aagtattaaa
     1381 atgtttattg gataataatg gacaagtgtt aacaagaatg caaattattg attatgtatg
     1441 gaaagatagc gaagaaactc cttatgatcg agtaatagat gtatatataa aagaacttag
     1501 aaaaaaatta caattagact gtataactac tataagaaat gtaggatata aattggagag
     1561 aaaatgaaaa aattaaagat atttccaaaa atgttcatac aaatattttc t
//
LOCUS       DACCYH010000189         1603 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.199, whole genome shotgun sequence.
ACCESSION   DACCYH010000189 DACCYH010000000
VERSION     DACCYH010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.199"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(213..1544)
                     /locus_tag="IVY85_002937"
     CDS             complement(213..1544)
                     /locus_tag="IVY85_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8045883.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT          551 a          292 c          233 g          527 t
ORIGIN      
        1 atttttcacc accaaaaacc tatctttgca tatactaaat agacgtatat acacagatag
       61 gtttgtcttg tgaaaaataa tgattatcct taccaaaaaa tgtttgtttt tctacatgtt
      121 cgtacttatc aaaaaatatg atccttattg aaattactgg ctatttatat tccaacgact
      181 tcttgtgtta atttttgtta tctcatagta aattatgttt cattttcact taatttagtt
      241 gtcacgtgtt cgtcaattcg taaaaacggt aaatatataa acacacttgc ggctataatt
      301 aatatttgta aaacagctgc tcgccagtct ccggctgttg ctaaaaatcc cgatataact
      361 ggtggcgtag tccaaggaat ttgcactact acatgattaa ttaatcccac agcagttgca
      421 aaatatgcta ctgtcaaaga aaaaatcggt gcaagcacaa agggaataat cagactgata
      481 ttaaatacaa taggcaatcc aaaaataata ggttcgctta tattgaatat agcgggagtt
      541 accgataatt tagcaaagtc cttataatct tttctacgac tgaagataaa tattgcaatc
      601 aataaagcaa tcgtatttcc cgatccaccc attaccgcaa aggtatcttt aaaggccata
      661 ttgataatat gtggaatttg ctctttgttt gcataagctg ccatattttc ttgcatattc
      721 tgaatcaaaa acggatctaa caatgaacct gatattactg cttgatggat accaaatcca
      781 aaaaatagat tagcaataga tgtaattaac aaaaatcctg gtaaaccagt tgtaatttta
      841 cttaatggct ctgtaactat cgtggtaatt agcttattta tatccatatt aaacaattga
      901 atgattataa atgacaataa agcaaacgag gtaatcgtaa taatgattgg gattaataca
      961 ttaaaagact ttaaaacagc gggaggaata tttccggata gctgaatctg tagtttctta
     1021 ttattagaaa gtttaataaa taattctgta gccgctaatc caacaaatat acctacaaac
     1081 attcctgaag ctcctgtata ggatataggt atcacacccg tcacttcaac agctttatcc
     1141 gcattttcag gagtaaacat catctgcata ggtgttaata caacaaatgt cgaaatagca
     1201 actaataccg ctgcaatatg atttgtataa tttctgcttg cacacatatg ataagatact
     1261 gctgctacaa ctaataatgt agttatgctt aatgtaccat tggcaattga agtaccaatt
     1321 tgttgtaacg tggtcaatgt actagtatcg acaatttccc ccatgaatcc tgttggttct
     1381 aagatcactg aattaataag tgtcacaaat cctgctaaca tcataaatgg cataaatgta
     1441 gcaaacgcat ctcgcaaagt tcttaaatgg atctgattcc ccaacttagt cgcaactata
     1501 ccaaattttt caaatacaat gtccttcctg ctacttttgc ccatattttt tctcctttat
     1561 gcttattgta atcgattact ttttccaccc aaataataca tca
//
LOCUS       DACCYH010000190         1507 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.200, whole genome shotgun sequence.
ACCESSION   DACCYH010000190 DACCYH010000000
VERSION     DACCYH010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.200"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            126..1058
                     /locus_tag="IVY85_002938"
     CDS             126..1058
                     /locus_tag="IVY85_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAP8045884.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1130..>1507
                     /locus_tag="IVY85_002939"
     CDS             1130..>1507
                     /locus_tag="IVY85_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8045885.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKNL"
BASE COUNT          664 a          172 c          234 g          437 t
ORIGIN      
        1 agaaagaaga tttaaatgat gaaataaatc aaaagtatga tgaaacatta aaaaatgtct
       61 attcttcact tatcgtttca aatatagaag aaacttcatc ttctgaaata aaaaataagc
      121 tttctttgtt aaaattaaaa gaaaaggaat atattattga cgatgctgtt tctgtccctc
      181 ttattttaaa taaacgagag aaaaataatt taaagaaaaa aatgctcatt ccatttgatt
      241 cagaagtgaa aggattactc aataaactaa ctatttctaa tatagactcg actagggaaa
      301 aaatcataaa aatatttgat aaaattaata atttattcaa gaatgacaat gctgaattaa
      361 ggaagggtct tttagagttt aaacttcaag aactagaact tcattactct tatttagtga
      421 aaattaccga agaaaaagaa caacaaaaag caataaaaga acagatgata gaagaggaaa
      481 aagttcgtag agaaattgat cgtgaaaaga aacgtattga taaagaacaa cgtcaattca
      541 attctgaaat ttccaaatta atcgcatata tgcaaaaagc taatgcagat gtggaaaaag
      601 aattatatgc aaataaaatt caagaactgg aagaaaaatt aaaagaatta gaagttatac
      661 gtgaaaatgt attacaaaga gaattaaata ctagagccgg ttatgtgtat gtaattagta
      721 atattgggtc ttttggtgaa gacatatata aaattggtat gactagaaga ttagaaccta
      781 tggatagaat aaaagaattg agtagtgcat cggttccatt tgaatttgac gttcatgcga
      841 tgattttttc agaggacgct ccgtctttgg aaactaaatt gcataatcat tttagaaaac
      901 aagaagttaa taaaattaat caaagaaaag aatttttcaa agtttcacta gacgaaatag
      961 agaaagttgt tttagaaaat tataatggaa cagtaacatt tacaaaatta gctaaagctg
     1021 aacaatatag acgatcttta gaattgtcaa aagattaaat atataaaaag ccttcgggct
     1081 ttttctttta acaattaata ggaacgtata ttcgaaagga atagaaataa tgtggttaga
     1141 aaaaataaat gacaaaaaat ttaagtacac cgaacgttat acggatccct taactgaaaa
     1201 aaagagaaaa gtgtccgtta ctttatctag caattcacgc caagcttgga atcaagctaa
     1261 cttacttttg aatgaaaaaa tcgcagaaaa aataaaacga aatgaagaat taccactaac
     1321 ttttggagaa ttaaaaacta aatgggatga aaaatacaag ccaactgtaa aagaaagttc
     1381 ctatcgaaca actcaggttt atctatcatt aatttcaaaa tatataaaag atgatgtact
     1441 tgtaaaaaac gttaatagta atcttataca agatatgcta gattactact attttgaaaa
     1501 aaattta
//
LOCUS       DACCYH010000191         1483 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.201, whole genome shotgun sequence.
ACCESSION   DACCYH010000191 DACCYH010000000
VERSION     DACCYH010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.201"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            64..972
                     /locus_tag="IVY85_002940"
     CDS             64..972
                     /locus_tag="IVY85_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP8045886.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1483
                     /locus_tag="IVY85_002941"
     CDS             972..>1483
                     /locus_tag="IVY85_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045887.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGV"
BASE COUNT          511 a          216 c          345 g          411 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taagaataca gaggttatgg aagcgactat agttgaaaat
      181 ggtcttccct acgatttatc agaatgtaag gtatattttg aatcagttgt aggtgggaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat taactatatt
      301 tttgataaat attccatgca gtgtttacat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcattaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaatcg aagatttaat tgcggatatg
      481 acggacttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgaac gttggagaca agaacaggaa cacagtttcc aagattggag agaaggacaa
      601 gaatcagact atttggcatg gtttgaatca atcaaggata ttttaaagac gattgatcca
      661 ggtggagtca tgttagccga actaacggat gcgcgagtag atattcaagg agttcgtcat
      721 gcgtctattt cagagcgttt gctggcagat atggactatt tatatcagaa attgcgagca
      781 acgcttttca cgattgaata cggggagatt gaagtgactg atattttgca ggatgatctc
      841 ttttcagata atcatgaagt cgaaaaagtt ggaactgtag cattcccgat tgaagaagga
      901 gccttgatca tcgcaaccgt tgatgatcca aagcaaaatg tgttcactct tgagaaagtt
      961 ggggtgatct aatggctaaa actaaacgaa tgatggaaac ggatgaaaaa acgggtgtac
     1021 aacgccaatt ctttccaatc acacatgctt ccgcagttct tgggttagaa gaaataatag
     1081 caggagaagc aacagtttta tctgttaatg gaaaaattgg agccgtcgtc attactaaag
     1141 aagatttggg attagagaat gttctcacag aattacccta tgcaagtgaa gaagatgacg
     1201 gcattatcac ggcagaaatg tatcaaaaaa ttttaaacag tggagaaggt gactacgtgt
     1261 tgccagttgc cactatcgac cgtttgggtg gcataaaagt tggtgaatta ttgacgattg
     1321 atgagacagg gaaagtctct gcagtcagac aatctgatgt caatttttcg ctggagttaa
     1381 aagaaaaact cgattcactg aaaaattata ctgctggaga aaacatcact attgatgaag
     1441 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgt
//
LOCUS       DACCYH010000192         1480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.202, whole genome shotgun sequence.
ACCESSION   DACCYH010000192 DACCYH010000000
VERSION     DACCYH010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.202"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            64..972
                     /locus_tag="IVY85_002942"
     CDS             64..972
                     /locus_tag="IVY85_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP8045888.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1480
                     /locus_tag="IVY85_002943"
     CDS             972..>1480
                     /locus_tag="IVY85_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045889.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          529 a          197 c          316 g          438 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt
//
LOCUS       DACCYH010000193         1442 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.203, whole genome shotgun sequence.
ACCESSION   DACCYH010000193 DACCYH010000000
VERSION     DACCYH010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.203"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(410..1024)
                     /locus_tag="IVY85_002944"
     CDS             complement(410..1024)
                     /locus_tag="IVY85_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8045890.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          469 a          278 c          240 g          455 t
ORIGIN      
        1 gtatcctgtt ggacaatttt gttatatcag gtaaacttca ttgcaataat gataacaaat
       61 gtactataat tactttgtaa agagttttgg ggaagactct ttcatcaacg tgtattaaga
      121 gacaagaaag aatccatatg gattctttct tgtctctttt tccactactt tttatttttc
      181 tgacactaca tattcttata actcatttct cataactgtg aaacaactta taaaaataac
      241 aaactttaaa caaaacaagc ttaaaatcaa agatgatcca tcttaaaata actaaaacaa
      301 aaaaagtagt cgttgttaat cctcattaat aatcgttttc tttttttcaa acgctccatt
      361 tttatgtgga acattatttt tttaaggaac tttgtcaaca tgtggataac tattttccac
      421 ttgtgtcctg gactagctgt tttgctgtaa aatacaaaac ttgctgcagc aaaaagatcg
      481 ttgcaagaga ggctgttccg cagatcgctg gatctagatt tggcaaagtc aagactgcat
      541 catgtaaatt ggcgtggttg tccaaaacca aatcagcaaa ttcaaataat cgcaaaccac
      601 tgctatgttg cgatttgata cttcgtgaca aatcaaatcc agtaacaatg attaatggat
      661 gaccattttc tttgatccac tgagctagtt cgatgattgc cgggtttctt ccttcattcg
      721 aaagaagaag aaaaatgtct tctgatttga cagaaagctg atcaataatt gcctgtcctg
      781 caccttctag cgtttcatag acacctccgg ctggattttc aatcaatata cttggtacaa
      841 gatccggggt ttgatcccaa aattcaaatg ctacaccact tagtgtgcgg ctagcatata
      901 tctgcaatct accaccttga ttaatttgtt tcgcgatcca agtgccagct tgaagcatca
      961 gcggctcttc tatccttaat ttctcttcca attggtgata atagtcaaaa taacataaac
     1021 tcatttatct ctcccgccta gctatacttg acatgaatag aaacagctgc ctaatctaag
     1081 gaagtacata aaaaaacagt agttagacag taacacatgt ggtctttgct gtttcaaaca
     1141 caaaaaaggc attgaacgaa tacgcccaat accttgacat agcaaataaa acaacgtgca
     1201 gaccatggaa aatatctaca taggggtaga attttcaccc tagattaggt acaataaatt
     1261 aggtcgatgc actgcattga ctatggcaaa atgatcggtc agggtgtatg tgtgtgactg
     1321 gtcattccct gattcttagc gccaactaag ggtcagggag ccttttttta ttcagctgtt
     1381 tctatctgaa tttattgtaa gtaatctctt atccaagtgc aagcaagaaa atacaaaaaa
     1441 ac
//
LOCUS       DACCYH010000194         1437 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.204, whole genome shotgun sequence.
ACCESSION   DACCYH010000194 DACCYH010000000
VERSION     DACCYH010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.204"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            105..1406
                     /locus_tag="IVY85_002945"
     CDS             105..1406
                     /locus_tag="IVY85_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8045891.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          473 a          288 c          264 g          412 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttct
//
LOCUS       DACCYH010000195         1426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.205, whole genome shotgun sequence.
ACCESSION   DACCYH010000195 DACCYH010000000
VERSION     DACCYH010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.205"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            41..451
                     /locus_tag="IVY85_002946"
     CDS             41..451
                     /locus_tag="IVY85_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8045892.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            465..821
                     /locus_tag="IVY85_002947"
     CDS             465..821
                     /locus_tag="IVY85_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP8045893.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          524 a          209 c          267 g          426 t
ORIGIN      
        1 ttactgtaga attggattat aattaaaaag gagtgagaaa atgataaaag tatatacttc
       61 gcctagctgt acttcctgta gaaaagcaag agcatggttg caagcgaatc aacttgaatt
      121 tgaagagaag aatatttttg ccgaaccgtt aacagaaagt gagatcaaaa agatcttggc
      181 agcgactgaa ggaggagttc aagatattat ttccactcgg tcgaaaattt acgaaaagct
      241 gggcatagat tttaacgaat taactttaaa gcaaatgatc gtcttattca aagaatatcc
      301 ttcattattg agacgaccta ttttgattga tgaaaaaaga atactaatcg gatttaatga
      361 agatgagatc cggtcatttg tccctcgtga aattcgatgt gttgccagaa atctgatcta
      421 tttacaaaac aataacatct taaattcata gggagggagc cttcgtggaa gtgaaagtct
      481 atggatcagc gcactgtgta acaagtctga aggcgaaaca atggttagaa caccatcaat
      541 tgccttatca atttgttgac ttagaagaac gcgatttaag tacagaagaa gcagaagaat
      601 tatgcagttt tgaagaagtg caagcagaac agctatttgc gacttggtct gaggcttttc
      661 acaacattgc gattgacctt tcctgtgtag gaaaaaaaca actgattttt ttatgcacac
      721 aacataccaa tctcctcaga cgtcctttga ttattatcga tgaagttcta tttattggat
      781 acaatgagca gttgatggaa cagcttatct taaataaata gactttaact gttttaatca
      841 agtcactgag catttcatac agttgtaaca gtcgaacact tagcatcaag cgtgttatgt
      901 gttctttttt tgactttatt tttctactat agagacatgc tcaatttatg tggtcaaaat
      961 tcaaatctag gtggggatat agagttaaaa atatattgac atgtgtaaat ttacatgata
     1021 tattcatttc tgttgtttta agtagcaaag caataaaata gtattttaaa aaacatataa
     1081 aatagttgtt gacatgttag aaatgacatg ataagataac tgagttgtta aaaaagaacc
     1141 acttgtgaag cttgattcta tcgaaaaaaa cttcaaaaaa gttcttgaca aataacaaat
     1201 gataagttat gataaatgag ttgctgtaag atttcgaaaa acgaaatcaa aaaaactttt
     1261 aaaaaagagt tgacaattac ttgtctctct gatatgatat aaaagttgct acggcagcca
     1321 aaaacgaatc ggggaacact cctgatgaag tagacctttg aaaactgaac aaagtaagac
     1381 aaaccaaatg tgtagggcgt cttgattcaa ttcaagacaa caaaca
//
LOCUS       DACCYH010000196         1424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.206, whole genome shotgun sequence.
ACCESSION   DACCYH010000196 DACCYH010000000
VERSION     DACCYH010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.206"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(150..1337)
                     /locus_tag="IVY85_002948"
     CDS             complement(150..1337)
                     /locus_tag="IVY85_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP8045894.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACCYH010000197         1355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.207, whole genome shotgun sequence.
ACCESSION   DACCYH010000197 DACCYH010000000
VERSION     DACCYH010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.207"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>1355
                     /locus_tag="IVY85_002949"
     rRNA            <1..>1355
                     /locus_tag="IVY85_002949"
                     /product="23S ribosomal RNA"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DACCYH010000198         1299 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.208, whole genome shotgun sequence.
ACCESSION   DACCYH010000198 DACCYH010000000
VERSION     DACCYH010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.208"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..487
                     /locus_tag="IVY85_002950"
     CDS             <1..487
                     /locus_tag="IVY85_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045895.1"
                     /translation="KQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILFFVGYVS
                     TKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKGIRSILK
                     NGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDLELPMKD
                     K"
     gene            508..720
                     /locus_tag="IVY85_002951"
     CDS             508..720
                     /locus_tag="IVY85_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045896.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
     gene            1153..>1299
                     /locus_tag="IVY85_002952"
     CDS             1153..>1299
                     /locus_tag="IVY85_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8045897.1"
                     /translation="MNPFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYR"
BASE COUNT          488 a          163 c          269 g          379 t
ORIGIN      
        1 aaaacaaatt gaacaatctg gaagaacaac aaaaggagtg aaggattttt tattgacgtt
       61 tattcacttt gtaaatggac acatgtattt gtttgaagtg ggtattattc tcttttttgt
      121 cgggtatgta tccacgaaac ttattaaacg ttcaattgaa aaagaaattg aaattgacct
      181 gattccgaca cttcttggtt atgagtacaa tgttgatttt ggaagatatg acgaattgat
      241 caatacggat atcgttcaca cgagtatcca tgaatctact gttaagggca tcagatctat
      301 tttaaaaaat ggccaggaaa tgaaagcaat agatcgaaga aaagagatct acaattttta
      361 tgaagtcgaa gcaagcctac gatatggcat cggaaaattg tggttagatg gaagtaatat
      421 tcgtcaaaac aaaccataca ttttttggaa cagtagtgat ttagaacttc caatgaaaga
      481 taaataatta tggagaatcg tttgaaaatg attcctctat acaggattgt gcttatagtt
      541 ggcatggagt taaaaacctt aaagaaggag cagttattca aagtgaaaga acgtcataat
      601 cagttactat gtagtaaaac gggtacgatg aacattgaat accgaacaga atatttagaa
      661 ttaacgaatc aaactgattt tttgttgatt cgaaagcaac aagagcaaga aaaaaattaa
      721 tggctactta tagccaattg attgatgaat gggaacaatt caaggaatct aaaattccag
      781 aacgtctcta aaagccttta tttgtaaaga gcataaggaa acgtgggaac actttttaaa
      841 aaaagtgttc caataggctg tgcctattac gtttctcaaa aataaatgtg ggaacaaaat
      901 atttaaaaat aggatagtga aaggatagtg aaggatagta aaaggagggt agtaggatag
      961 caaaaaggat aggctccttt tcgctatcct ttttaaattg gaggaattaa tatggaagta
     1021 agaataagaa atgtggatga gaaaatcgta agagaaattg atcgaagatg tgacgaacgg
     1081 ttctgttgca aagttttaaa tctactatca aataaggtag aataatagaa aaagatagca
     1141 ggaggaatga cgatgaatcc ttttaaagga aagcaatttc agcaggatgt gattattgta
     1201 gccgtgggct actatcttcg ttataacctt agctatcgtg aagttcaaga aatcttatat
     1261 gatcgtggca ttaacgtttc tcatacgacg atttatcgt
//
LOCUS       DACCYH010000199         1279 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.209, whole genome shotgun sequence.
ACCESSION   DACCYH010000199 DACCYH010000000
VERSION     DACCYH010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.209"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..383
                     /locus_tag="IVY85_002953"
     CDS             <1..383
                     /locus_tag="IVY85_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8045898.1"
                     /translation="FSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKG
                     CPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVG
                     YRNQRLAQRILDNELGCANASEAV"
     gene            complement(367..960)
                     /locus_tag="IVY85_002954"
     CDS             complement(367..960)
                     /locus_tag="IVY85_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HAP8045899.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVSGGMATVFLTNF
                     IRCRTSFRMLFRLSLTMSLLGCDDLRQELTVKVRLPR"
BASE COUNT          393 a          251 c          216 g          419 t
ORIGIN      
        1 catttagccg tatcaaacaa cctctgacag aggttgagat ttttcatact gatcgtggaa
       61 aagagtttga taaccaagct attgatgaat tattaacaac ttttgacatc aatcgatcat
      121 tgagtcataa aggctgtcct tttgataatg ccgtagctga atcaacttat aagtcgttga
      181 aagtagaatt tgtctatcaa tacacatttg aaaccttaca acaattggat ttggagttat
      241 ttgactatgt aaattggtgg aaccaccttc ggttgcacgg tacacttggc tacgagacac
      301 cggttggtta ccgtaaccag agattggcgc agcgaatcct tgataatgag ctcggatgtg
      361 ctaacgctag cgaggcagtc taactttaac tgttagctcc tgccgaagat cgtcacatcc
      421 gaggaggctc attgtcaagg acaatcggaa tagcatacgg aaagaagttc tgcaccttat
      481 aaaatttgtc aaaaaaactg ttgccattcc accagacact tgcttaaagc gaaaaatttc
      541 ttcaatgtcc atgcctaatt ctttggatat tctcttgtct gaccatccat tttttactaa
      601 tctttggatg acaatttcca ttcctcttat ttcatgggtc cctcttgcac gattgtgtcg
      661 aattgttgaa gccatacgtt cttctatggg tttatcaata actgttaccg gtacaacttt
      721 taagttgagt ctttccttag caacacgata cctatgaagc acatcaacca agatatactt
      781 ctcttctttt tccttgtaat aacaaactaa tggttgagta aagccatctt caatgataga
      841 aagctccaat aagttcattt ccggagacgc tactcgattg ggattataat cgttcgcctg
      901 tattttggta attggaacaa gccgaacgtc catcacaggg aaagttatgt ctttattcat
      961 acgcttgtat accctacttt cttaaaaagt agggtatact caagaatccg attaattatc
     1021 ttctcaattt ggataatata tctcatcagt cttcttgtca ctttatcaac atgattttgc
     1081 tgcaaaatgg gataccgaca gtactggact ggtaatcagg ataaaagcaa aaataattaa
     1141 tttgtatcct aaaaaatcca taaaaattct ctcacatttt cgggtattaa ttcgagaatc
     1201 acacctataa atttattact atataaggga tacttaaacc aaatacccca tctaattatt
     1261 ttataattta tatttttga
//
LOCUS       DACCYH010000200         1279 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.210, whole genome shotgun sequence.
ACCESSION   DACCYH010000200 DACCYH010000000
VERSION     DACCYH010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.210"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(18..317)
                     /locus_tag="IVY85_002955"
     CDS             complement(18..317)
                     /locus_tag="IVY85_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045900.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(323..553)
                     /locus_tag="IVY85_002956"
     CDS             complement(323..553)
                     /locus_tag="IVY85_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045901.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(803..1003)
                     /locus_tag="IVY85_002957"
     CDS             complement(803..1003)
                     /locus_tag="IVY85_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8045902.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          443 a          209 c          192 g          435 t
ORIGIN      
        1 tttttaggta gcctacttta atctttgtat ctttctggat caacgaaagt atactttata
       61 tagtcataac gccgatgatc gcttcgagcg tctagcacat cagtcacgat atcaaacaaa
      121 aaatatacgt ctttcttcat tctagttttc gcagcaggga ttttaaagta gttcttatta
      181 gaatagtaga gattaattaa taagctatct tcgattgcta aaaagaaaac ttctgaatcc
      241 cacaccttat aaaaatcttt gacaaatcta ttcgaaggat caaatttaaa ccataattgc
      301 gtctcattaa aaagcataac cattactccg atctattttt aaacttagtc tctacctcta
      361 atatatatcg agtttttatt ttgccttcag agaatactgt ttcttttttt gcagttacag
      421 gttgtttatt ttcggaaaaa gctaatatag ctaaaattga aacatccatc tggaatttgt
      481 ctttttcgtt gctttgctca taaaaatctg catattcatc actaatattt tttctaataa
      541 attcttccat cataaagatc acctcgaaaa gagtatacga acaaacgttc tttttgtaaa
      601 ggtggaattt attatactaa ataaataggt gaacaagtac ttgtgccaaa ttatgtgcca
      661 aaaaaaatcg aatttaataa aacacaaaca aaaagaaatc ctattatgat aacatttctt
      721 ataataacaa acacaacaga agacgtgtaa tagttagtca ggaacgtaca aataacccct
      781 gtatcctttg cggtacaggg ggttattttg tataagtaga cattgtagtt tctaaactgc
      841 tatgtcctaa acgtttcgat acgctaagta tatttactcc ttgataaagt aaaatagatg
      901 catgcgtatg tcttagtcca tcaataaaca gtcgaagaga aaatagggaa aaattgatta
      961 ctaaagaaaa acttactagg tcgacggtgt agttgatttt catacttcaa tagattcgaa
     1021 ttaaacgatt tcgattggct tataccagtc ctatccaacg aaagatatat cgttcttaac
     1081 tctagcggta taacttgaag ctttacgtaa agccaatggg aatgaactgg agatgacttt
     1141 ttattatttt tagtaatagc tgatgggtta atacttacaa ctttcctttt caaagtacca
     1201 tataaaaaaa cggtggggtt gtttagcgaa ataaaggatc taagccaata gcttagatcc
     1261 tttattctat gctttatat
//
LOCUS       DACCYH010000201         1273 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.211, whole genome shotgun sequence.
ACCESSION   DACCYH010000201 DACCYH010000000
VERSION     DACCYH010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.211"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            854..1066
                     /locus_tag="IVY85_002958"
     CDS             854..1066
                     /locus_tag="IVY85_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045903.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT          477 a          189 c          209 g          398 t
ORIGIN      
        1 tatattaaat attatacaga taataacgga atattatcat cgaaacagct aacaaaattg
       61 tcataaaaaa cgagatggtt gatattagaa cttgtgtttt tgtttaggtc tagagataaa
      121 gagagacaat ttccaaatat atgtaatgaa tacaaaaaga cgtttgtcgc ggattaccgt
      181 tacaaacgtc ttttattgat tttccgagat ctcatcagtc gccatttgga atggttcact
      241 ccttatctag attcgtttaa tgctcggact gcttgttcga ttgtaatacc aaacgcaacg
      301 ttattttcgt tttgtgcatc aatcgccata aaacggttag aagttgtatc aaaagctacg
      361 cggtattgca tatttgtttt tgtaaacgtc ataaaattca tctcctttgt aatgtaattt
      421 agttcttgaa agtttccttt aaactttcga acttatacca agtttagcat cgtacgtttt
      481 ttgatgcaat taaaaagcat ttatcatttt ccttatttat tttttcttaa atggtttagc
      541 tattttcttt taatccgata agtagcccaa tcatcaaggt tacaaatact attgcttgaa
      601 tataaatcat atgctcacct ctttataaat aacttagcaa tataaagaaa aaaacgcacg
      661 aacaacatag gaatataaac aaacgttttt gtcacaacgt ctaagatcgt gttaaaaaat
      721 agttatctaa aaagcagaaa gatactcaaa ataaaaaata aaaaaatgga taaaaacgtt
      781 ttcaaataca aggtaatttg ttattataaa ataaagaaaa aacaaaataa aaataaagaa
      841 aagagggaat gaaatggtaa aagcagaagt aggaagatct tatatatgca aaccgatcgg
      901 agtatctcat gaactaatcg gctgtattga acgtacatat atcaacacgg ctgtcgtgta
      961 tgttgaaagt tatgaccaac gagactatca acttatcaat gaaagcaagt atcgcatgct
     1021 cgtaaaattc tgtgatatta gcgcacctgc tgaattggtt tcttaaacag tatttttgag
     1081 aattcgcaag aactccctta taatataaat aaagacagaa gggagcgaga agatatgcca
     1141 aaaagaaaaa gttcagggaa aatgggaatt attcttgcag tgatctctgc aattcctgct
     1201 attttagcta ttgttcgtgc gatgaaggaa tcaagagaaa gttaagcaca cattatgagc
     1261 gtcaaacaaa aaa
//
LOCUS       DACCYH010000202         1266 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.212, whole genome shotgun sequence.
ACCESSION   DACCYH010000202 DACCYH010000000
VERSION     DACCYH010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.212"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            84..1253
                     /locus_tag="IVY85_002959"
     CDS             84..1253
                     /locus_tag="IVY85_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HAP8045904.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          465 a          187 c          216 g          398 t
ORIGIN      
        1 atattttgtg taaatagaaa aaaggaagtc ccttctgtag aatagagtta ccacaacaca
       61 ttcacagaaa agaggacttc catatgaacg attttactac agaaattatg gaaatactaa
      121 tcaataaagg tgatttggat gatttgtttc gtcgtcacct agaattggct attaacgctt
      181 tattacaagc tgaattaacg gtatttcttg actatgaaaa gtatgataga gctggattta
      241 attcaggtaa ttcccgtaat gggaattatt cgcgctcttt taaaacagag tacggtgaat
      301 tgaatttagt aatacctaga gataggaatg gagacttttc tcaacaaact ttacctgctt
      361 ataaaagaac caatgactct ttagaaacca ctattattca gctagttcaa aaaggaatta
      421 ctatgtccga aatctctgat ctaatcgaaa aaatgtatgg tcattattac aaaccacaaa
      481 ctatttcaaa tatgagtaaa attgtgtctg aagatgtttc ggcatttaaa gaaagaactt
      541 tagaagctaa atactcagtc atttttatgg atgctactca tattccctta aaaagacaaa
      601 ccgtatcaaa agaagctgtc tatatagtaa ttggtatcag gttggatgga actaaagagg
      661 ttctaggatt tactatagct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
      721 atctaaaaga ccgtggttta gaagaggttt tgttagtcgt aacagatggt ttaagtggta
      781 ttgacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
      841 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
      901 tcaaattagt ttaccaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga
      961 tagataaatg gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat
     1021 taacctttta taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg
     1081 aaggttttaa caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag
     1141 aatctctgga gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc
     1201 gagttcataa aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc
     1261 aacaga
//
LOCUS       DACCYH010000203         1253 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.213, whole genome shotgun sequence.
ACCESSION   DACCYH010000203 DACCYH010000000
VERSION     DACCYH010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.213"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            34..906
                     /locus_tag="IVY85_002960"
     CDS             34..906
                     /locus_tag="IVY85_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8045905.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          376 a          230 c          302 g          345 t
ORIGIN      
        1 tgatttcatt ctaaaataaa gggagaaatg tctatgctat acggtgcaat tgaagcaggc
       61 ggcacaaaat ttgtgtgtgc cattggcgat gaagaaatga cgatcaagga aagagtgagc
      121 tttccgacaa cgacacctga ggaaacaatg cctttagtca ttgacttctt caaacaatac
      181 caagcagatt tagcagggat tgggattggc tcatttgggc ctatcgatat ccatcgcgat
      241 tccgccactt atgggtacat tacctcgacc ccgaaattgg catggcaaaa ttttgatttc
      301 atcggtacca tgaaaaaaga atttccgatc ccgatttctt ggacaacaga tgtcaatgcg
      361 gctgcctatg gtgagtatgt cttcggcagt ggcaaaggat tgagcagtgt cgtttactat
      421 acgatcggta cgggtgttgg cggcggtgcc ttacaagaag ggcgctttat cgaagggttt
      481 agtcatccag aaatggggca tatgctcgtc gtccctcatc caaaagatga ctttgctggc
      541 agctgtccat ttcatggcaa ctgtttagaa gggatggccg ctggacccgc catcgaaaac
      601 cgcttaggga aaaaaggaca agaggttgca gaagatgatc cttattggga aatcgaagcc
      661 tcctatattg ctcaatgtgc ctacaataca accttgatgt tttctccaga tgtgatcatt
      721 tttggcggtg gtgtgatgaa acaacgccat atgctgcaaa acgtccacga cgcatttgca
      781 aaattggtca atgggtatgt cgaaacacct gagttaagca aatatatcgt gaccccagca
      841 ttagaagaca acgctggaac gctcggttgt ttggcattgg ctcgagaagc ggtcttacat
      901 tcataaacga aaaaaggctc ttcgacattt agtgttggta gagaaagata caactgaaaa
      961 tttagtcata accagttagg cagagtgctt aactggtttt tgttttactt ggaagatcaa
     1021 gccttgaatg agataaattc ttgctcgaaa gttatggaaa tttcgatagc cgtaggccac
     1081 ttgtattgtg caatagtttt ttacaaaaaa aagggggtgc ggatattttt ttggaccttt
     1141 ttaacaaata ggttctgttg caaagtttta aatctactat cagataaggt agaataatag
     1201 aaaaagatag caggaggaat gacgatgagt cattttaaag gaaagcaatt tca
//
LOCUS       DACCYH010000204         1252 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.214, whole genome shotgun sequence.
ACCESSION   DACCYH010000204 DACCYH010000000
VERSION     DACCYH010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.214"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            160..1200
                     /locus_tag="IVY85_002961"
     CDS             160..1200
                     /locus_tag="IVY85_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP8045906.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          505 a          179 c          207 g          361 t
ORIGIN      
        1 ctttaaaggt agtacataat tcattttatt tctgatttta ggtagtattt aattaaaaaa
       61 cagttttaat gcgtttgcat tttttattta tctttgctat tttcgaaaag agtaggtaat
      121 tttacataaa taaaacaacg tgaaagtaga ggatctacaa tgactgattt taaatttttt
      181 aaggcggata gagtttacaa cgaattattt tatcaatttc caaaagtctt tattgtttct
      241 gacgaataca aaaaaatgaa agattcaact aagattgcct atatgctttt aaaagcaaga
      301 ttagagatcg caatcagcaa acggcaaatc gatgaagaag gtaatgttta ttttacttat
      361 acgacaaatg aactatgtag agtattaaac tgccaaaaac aaaaagcgat agcaatcaaa
      421 aaagagttgg aatcctttgg tttattatta caaaagcaga tgggatttaa caaacagtta
      481 gggaaaaata atcctaatag actatatcta gcagaattaa aagtctcaga aaatgatatc
      541 tacttactcg aaaaatttga tagagagaat agggaaaacg ttgataaatc agagggtatg
      601 aaaatcatac ccaccctcga cgaaaaatca gacgctgaat cccttggggc tcaagagggt
      661 atgaaaatca taccgtgcca aaacgttgat aaatcagagg gtatgaaaat cataccagaa
      721 cttaataata atatattaga cactaataga cacaatatag acactgaaaa agaccgccta
      781 caagatcaat tgttgttaga caattttgag acaattatga caaacgacag cattgctacg
      841 tttgtccctg aacgagtatt aaatttgata aaaacatttt cttcaagtta cagtgaagct
      901 caaaaaaccg tccagactat tcataatgca aagaaaaaag ctgaaataga aagtgatatt
      961 tcgatagttt ttgaagaact cgatagttat tatgtcaatg cagaacaaga attatacacg
     1021 acactgttaa aagcctatca aaaattaaaa accgaaaaag tcgaaaatat ccagaacctg
     1081 atttttgtct atgtaaaaaa ttggtttatc gaaaaaccaa tagctgctaa agtatcaagt
     1141 gaaaaacgtt tgaattatgc aagctcccca agcactatta cgaaagactg gttagagtga
     1201 aactttactg acaaagtgat tgttgcttca tggagtaaca ttaaaattaa aa
//
LOCUS       DACCYH010000205         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.215, whole genome shotgun sequence.
ACCESSION   DACCYH010000205 DACCYH010000000
VERSION     DACCYH010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.215"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            200..742
                     /locus_tag="IVY85_002962"
     CDS             200..742
                     /locus_tag="IVY85_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045907.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            832..>1246
                     /locus_tag="IVY85_002963"
     CDS             832..>1246
                     /locus_tag="IVY85_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP8045908.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DACCYH010000206         1245 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.216, whole genome shotgun sequence.
ACCESSION   DACCYH010000206 DACCYH010000000
VERSION     DACCYH010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.216"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            70..1026
                     /locus_tag="IVY85_002964"
     CDS             70..1026
                     /locus_tag="IVY85_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045909.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
     gene            complement(1080..>1245)
                     /locus_tag="IVY85_002965"
     CDS             complement(1080..>1245)
                     /locus_tag="IVY85_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8045910.1"
                     /translation="RKDGLPKEMEFNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFL
                     SYVNGKFCLA"
BASE COUNT          430 a          186 c          212 g          417 t
ORIGIN      
        1 ttgacaggga atcctaaaaa cattcggctt tttttatata atctttttaa ctcaacagag
       61 ataagttttg tggaattgta tacgctacct ataattataa aaacagaaaa aatgttaaaa
      121 gatttaaaac agtattatcc tctgtctgct tatgaaaaaa agaaattatt ccacttttta
      181 gcaatcaacc tgttggctga acaagctgat tctttagata ttgctagcaa tcagtcgcta
      241 tttttttcgg atataggatt ttaccactta aaaggagtat taggagaaaa atccgttatt
      301 tttatcaata agacactctc ttggctttat cttcatgata aattggatca aaaatacttg
      361 agaaaaaatg aagatgaaac aatcgaatgg ttgaatgaac gattcattga aagttttgaa
      421 aatcagttta atctgttgcg agaagagacg agccaattga tccgaagaga gttaaccaaa
      481 atacatttta aacttttgca ttacactatt aataaagctg atgaatttcc acttgatgtt
      541 tcttttttta gagaaaacta tccagcattt tattgctatt tgatagagta tataaatgat
      601 ctaacgtcaa aacgcaaaga gttgcttcaa gataaatttt ttcttttttt cagttattta
      661 ttattattga tcaatcacat acccattcaa ctgatttctg aaccagtaaa gatcatcatc
      721 gatttttctt atggtgctgc ctataatcaa tttatcaaga aaaatttaag cttgtacatt
      781 aatctgaaca cggaagtgat agaggcagga aagcctgagt tcccggatgt tataatcacg
      841 aataggaata atctatatga agaaagtgca tctcaagtag tggtttggct cgatccgcca
      901 agagcagtag actgggggaa tcttacaaag attttgttga ctatccaaga agagaaatac
      961 aaagacagca aaattgacaa gcaaatagac gattttcctg aagaaatcat cgacaggatc
     1021 gaatagtgaa cttcttttag aaagtccgac tgctttttct aaaaatatta tttgtcaaat
     1081 taagcgagac aaaactttcc atttacgtaa ctcaaaaaaa cttctaatgg tgtttggtaa
     1141 tttagtgatt ttctagggat atggtttctt ttggacgcaa cggatgagat gaatccttga
     1201 ttgacttggt tgaattccat ttcttttggt agtccatctt ttcgg
//
LOCUS       DACCYH010000207         1200 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.217, whole genome shotgun sequence.
ACCESSION   DACCYH010000207 DACCYH010000000
VERSION     DACCYH010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.217"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            76..>1200
                     /locus_tag="IVY85_002966"
     CDS             76..>1200
                     /locus_tag="IVY85_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP8045911.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLL"
BASE COUNT          397 a          234 c          233 g          336 t
ORIGIN      
        1 ggtattaaaa aacatatcgt tctctagtat gattaaatca ccacaaaata aacaactgag
       61 aggacgatac gctatatgaa tgattctatc aaaaaaatgc tgagaataac agagaaagat
      121 ttgatgatta cagaggtctc ttacgagacc cttcagaaga aaaagacgtt ggtcgtcgat
      181 gctgttctct cgcctactcc tcgtgcttgt agaagttgtg gttctactgt ggtagatgga
      241 aacgggaaag caattatagt gaaaaatgga aaaaaggaaa cgattgtccg ttttgaacaa
      301 tacaatcata tgcctttggt tatgcgccta aaaaagcaac gctatacctg taaaaactgt
      361 cgcacccatt ggacggctca aagttatttt gtccaaccca gacattcaat cgcaaatcat
      421 gttagatata aaattgcttc tttactgact gaaaaagtat ctttatcttt tattgcgaaa
      481 agctgtcagg tatctttgac caccgttatt cgtacattga aagagtttaa aagctattta
      541 ccaaagcaat ctaagaagat cctccccaga gtattgatgg ttgacgaatt tcgttcgcat
      601 gcttccatag aagataagat gagctttatt tgcgcggatg gcgaaacagg aaaattaata
      661 gatgttttgc ctacgcgtaa attacctcga ttaacaagct atttcttagg ttgtaccaat
      721 ccagaagaag tagaattctt ggtgacagac atgaacgccg cctacttcca gctcaccaaa
      781 cgtgttctgc caaatgcgaa agtcgtgatt gatcggtttc atattgtcaa acacatgaat
      841 caagcgttca atgagttgcg tatccgtgaa atgaatgaac ttcgtaaagc cggacagaaa
      901 agccaggcag aaaaactgaa aaagaactgg cgctttttgc taaaaaatcg tgcaaacatc
      961 aaccattatg aatacaaaac atggaaaagt ttccgagcac caaaataccc atttcttact
     1021 gaagcaatga tgattgatcg attgcttgag ttttctccgc ccctaaagga ggcgtatccc
     1081 ttttttcatg aattagttga agcctttcga gacaaagacc ctgacttatt tttctcctta
     1141 ttggcagaac ttcccgaaac gttagatgac agctttcggg aaaagcttca aaaccttctg
//
LOCUS       DACCYH010000208         1195 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.218, whole genome shotgun sequence.
ACCESSION   DACCYH010000208 DACCYH010000000
VERSION     DACCYH010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.218"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..1076
                     /locus_tag="IVY85_002967"
     CDS             <1..1076
                     /locus_tag="IVY85_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8045912.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DACCYH010000209         1185 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.219, whole genome shotgun sequence.
ACCESSION   DACCYH010000209 DACCYH010000000
VERSION     DACCYH010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.219"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            42..302
                     /locus_tag="IVY85_002968"
     CDS             42..302
                     /locus_tag="IVY85_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045913.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(664..903)
                     /locus_tag="IVY85_002969"
     CDS             complement(664..903)
                     /locus_tag="IVY85_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045914.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          421 a          165 c          219 g          380 t
ORIGIN      
        1 atcattttaa ataatggaaa aacattaagg atggatgaaa tatgaataac gaaactatca
       61 ttttaggaaa aaaatatatt tgccaaccta ttggcttgaa acaaaaagtg gttggagaag
      121 taatcagtaa attagaaaat tgtgttgtga tctgcgtaga cgaatatcat gacgtcgatc
      181 gcaatgaaat attagaaaaa tgcggaaaag tagtggcaaa atatactcac gtttatgagt
      241 tggaaacaac agaatatcta tataactata gcaacaaata tgaacatact ttagtttcat
      301 aaagatttaa ataaagtgcc ttcaaaagcg agaagaccaa gcttttgaag gcactttatt
      361 tagttggcag tgattgttca tgatttttga tttattcaaa aggttcgcct agagaatctg
      421 tctctgtaac gaggcctggt ggagaaactc gataaaaacc tatactgcca gaactgtcct
      481 attttcggat agacaaacta gtagttttaa aaagatcatc cgatccaatc atttcaaaaa
      541 atgcaaaact gatatcagga tcatttagct caggcttgtt atcgtaaagc atttgtaaag
      601 gttgcattta gttaggagta aacgcgaaac tatcatcatt atttgctgaa gaagatgaag
      661 aagctaattc agagtttgtt tgaaataaag gagacgtgct gacagtttct ttaccctgtt
      721 ttttgcagag gtagaaactt ttatatcgga agtttctacc ggagaatttt tctcatttgt
      781 agaaattgtt gaatcgatac tattcgaaat ttgcgtatct gaaggtatgt tctttgatgc
      841 agaagtccca caagcagtaa gtaacaataa atttgataaa ctgataaaca gaattctttt
      901 cattatgtac acactcctta tagtatcgtt tagcatacac ctatgctcag tattaggcaa
      961 aatcaatgca aaactatcaa gtcttttata gtatctatta gtcttagtaa tacatatatg
     1021 gagagggtga tatcccagtt caaaccagat acattggtta ccatctgtat taacgattat
     1081 gcctctaaac gcttttttta tgaaaaaatt tataagtgaa aacgtaggta tgatattatc
     1141 tagaggtaat ttttttgggt atttaaatag ttgctaggag gatag
//
LOCUS       DACCYH010000210         1176 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.220, whole genome shotgun sequence.
ACCESSION   DACCYH010000210 DACCYH010000000
VERSION     DACCYH010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.220"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..400
                     /locus_tag="IVY85_002970"
     CDS             <1..400
                     /locus_tag="IVY85_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045915.1"
                     /translation="YFLLNLDSVNSNAQIKDQRVAEHNNKKMSLTKNIYSMSDEELYK
                     EFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSELVSYKQNHRQFIDVNTDSN
                     AISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            414..1103
                     /locus_tag="IVY85_002971"
     CDS             414..1103
                     /locus_tag="IVY85_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045916.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
BASE COUNT          414 a          159 c          241 g          362 t
ORIGIN      
        1 ctattttctt ttgaatttag attctgttaa ttcaaacgct caaataaaag atcaaagagt
       61 agctgaacat aataataaaa aaatgagttt gaccaaaaat atctattcta tgtcagatga
      121 agaattgtat aaagaatttg gtgatttatc cggggaacag ttagaagaaa gtattccagg
      181 aattagtgac tcagataaaa ttgttgtctt gtctgctggt gggcaattaa tgggagatgg
      241 caaatccgaa ttagttagtt ataaacaaaa tcacagacag tttattgatg ttaatactga
      301 ttccaacgct atttcgatta aggatctaaa agagctttta aatagtaaaa gaaatgaatt
      361 aaagcagata aaagaaacta attcagtgga attagagtaa aggaagattc attatgaaaa
      421 agatttttgg tgtttctgtt gggctactta tgatgttaac atttggaaat gcagtatatg
      481 ctgctgagaa agaagttatt tcagatccag agcagtacgc ctcacgatac tttgctgatt
      541 ttgaagaaga agataaagta cttgcattgc cagaaggtgg ttttttaaaa ggggaagcta
      601 aaattgtatc tatggataat ccagatataa ttcaaggttc atataattca gaaactgatc
      661 ctgcttcagt ttcagtgaca gaagcaaaaa aaattgttgc tgaaaaattc aaaaatgata
      721 ttgaaactac tccaattatg cctcgaggag catttccacc cgatgttggt actagcggac
      781 accttcttgt actagcagga ggaggaagct ataaatcaca gccatttagt ggtgcgggat
      841 ggcgttttgg aggtatgggt gtaaaaccag ctgatggtac aggcaacttt ttacgttgga
      901 aatcctacat tgacgatgga agagtaggaa atgtaaatca agccaacgca actaaatctg
      961 gttctattca aggaatggca ttgtatgctc gtgaaggatt caaatacttt gcaccaaacg
     1021 ggcctggttg gacaatgaat ataggtattt attacactta taatcctgtt gcaggtacgt
     1081 attatgttgt tcaaaatatt taataaaaca gcacaaagag atttctttgt gctgttttat
     1141 tatttttagt tgttgacaaa cactaaaata taaaat
//
LOCUS       DACCYH010000211         1164 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.221, whole genome shotgun sequence.
ACCESSION   DACCYH010000211 DACCYH010000000
VERSION     DACCYH010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.221"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..969
                     /locus_tag="IVY85_002972"
     CDS             <1..969
                     /locus_tag="IVY85_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8045917.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          394 a          207 c          232 g          331 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt accc
//
LOCUS       DACCYH010000212         1160 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.222, whole genome shotgun sequence.
ACCESSION   DACCYH010000212 DACCYH010000000
VERSION     DACCYH010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.222"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            14..1141
                     /locus_tag="IVY85_002973"
     CDS             14..1141
                     /locus_tag="IVY85_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8045918.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          419 a          200 c          224 g          317 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa
//
LOCUS       DACCYH010000213         1156 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.223, whole genome shotgun sequence.
ACCESSION   DACCYH010000213 DACCYH010000000
VERSION     DACCYH010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.223"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            237..>1156
                     /locus_tag="IVY85_002974"
     CDS             237..>1156
                     /locus_tag="IVY85_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP8045919.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     QEGKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQ
                     LRRGKATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYK
                     YRELLKQESQNDELTNHLRETFSESADDLKR"
BASE COUNT          397 a          183 c          249 g          327 t
ORIGIN      
        1 aggagtgcta taatcaaaga aaacgagaaa aaagagcaaa ataaaagccg aacggtgttg
       61 gaagcaccct tcggctaatt acggaaacta ctcacctagt tccgtaactc aaattcatta
      121 tacgaatatt tttaggtttc ttcaagctag agatttttgg atatggattt ttagagttac
      181 gtgaactctg gaaattcttt ccaaaaatct cttttttctt tgaggaggaa caaaatttga
      241 gtaaacgtgg cacgatcata aatgagtggg aaaaacttaa ccgcatatct aacaggcggt
      301 taaatgcaca aactatgtgc acccccttaa aaatgcaatg gtcaattgac cgaattacca
      361 ttgttggtac attaagggaa aatatttatt atcacactcc ccatgatgtg ttgattttag
      421 attttgaaca gcttatgcgt ctaaacgaag gtaacggata tttaaaagcg gttggaaata
      481 atggctggca attactagac caacatgagg aaaatatagc ttatattgag attcttaagt
      541 ttcaagaggg aaaagggcgg attgacttta accccaataa aattagccaa tttctggcgg
      601 gctcgatgaa gaactttatc catgatttat ttttagaacc acatttctca cgtgcagata
      661 ttgcttgtga cattattgac gtgccagatg atttcattac ccaatatcgg gtggttgatc
      721 ctgtttcttt caaaccaatt tacggtcggt ctgggaaact ggaaacagca tattggggaa
      781 gtcgtgctag tgaacgacag attcggatgt ataacaaaaa attggaacaa gaaagaaaac
      841 ggaaagttgt tccgaaagaa atcgatactt ggtggcgact agaattgcaa ttgcgaaggg
      901 ggaaagcaac tgattggcat gcaatggttc atgaaagttt agatagcttt gcaagtcctc
      961 actatttgcc agaagatgtg aaagtaactg acagaatgat gattactgct cttataacgg
     1021 aacatagtta ctgggggcaa attcatagaa attcaaaata taaatatcgt gaattgttaa
     1081 agcaagaaag tcaaaatgat gagttgacga atcatttgcg agaaacattt tcggagtcgg
     1141 cagatgattt gaaacg
//
LOCUS       DACCYH010000214         1153 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.224, whole genome shotgun sequence.
ACCESSION   DACCYH010000214 DACCYH010000000
VERSION     DACCYH010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.224"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            41..472
                     /locus_tag="IVY85_002975"
     CDS             41..472
                     /locus_tag="IVY85_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045920.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            474..611
                     /locus_tag="IVY85_002976"
     CDS             474..611
                     /locus_tag="IVY85_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8045921.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            648..941
                     /locus_tag="IVY85_002977"
     CDS             648..941
                     /locus_tag="IVY85_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045922.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            938..>1153
                     /locus_tag="IVY85_002978"
     CDS             938..>1153
                     /locus_tag="IVY85_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8045923.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGG"
BASE COUNT          431 a          166 c          239 g          317 t
ORIGIN      
        1 tcatttgtta tcacttattc aatttaaggg aggaaatcta atgaaagtag tttacaaatc
       61 aatcaagcct tacggattcg agcaaatcat tttgaacaat caagaaaata tccctgaaaa
      121 ctgtacagag attaaaccac cagttcctaa ctggagacca agatttgatt ttgataaaaa
      181 acagtgggtt gagttagcta ctgaagaaga gaaaaaaggg aatgctgttg atagcgtaga
      241 tgaattggct aatattaagg cattatacga aacgctgaag gcagaaaatg atgaattgaa
      301 acagctaaat tctaaagcaa tgctaaacaa tgtagcaatc aagcaagaaa atgtcttatt
      361 gaaagaaaag tcagaaagtt tagcgcagtt gaattcaaaa acgatgcttg cttctgtaca
      421 aaataccaag gaaatcgaag aaatcaaaaa acaattacaa ggtgggaagt aacatgtatt
      481 catatgaaga tatcaaacta atgtatgact ggggctgttt cactaacgaa caagtaatgg
      541 ttttcgttcc attatgcatt acagacgaag aagcagataa aatcattaat aaagataaga
      601 gcgcatctta attgatgcgt ttttatttaa ggtaaaggag ttgtcacatg attaatttag
      661 gggaatgggg aatgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa
      721 ttagaccgat tgctgcatct ttctcgaaaa ttactgagac tctttcaaaa gtaagccaca
      781 atttagattt gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg
      841 aagaactaaa gaaacacgat gaaagattag atacacatac agaaaaattg gtggaacaca
      901 cgcaacaaat caaaactttg tttagggaga gaagaaaatg aataataaaa cgttcgaagt
      961 actaaaatgg ttcgcactgg taattattcc cgcactagct actttcgtgg ggttagttgg
     1021 taaagcgctc aattggcagt acacagatat ctgtgttgtc atcattactg gttttggcgc
     1081 gtttttaggg agtgtgttgg gtgtatcaaa tcgaacctac aaaatgttct cggctgaaag
     1141 cgaagaagga gga
//
LOCUS       DACCYH010000215         1144 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.225, whole genome shotgun sequence.
ACCESSION   DACCYH010000215 DACCYH010000000
VERSION     DACCYH010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.225"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            4..963
                     /locus_tag="IVY85_002979"
     CDS             4..963
                     /locus_tag="IVY85_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8045924.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRADVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          386 a          211 c          230 g          317 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagagcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt
     1141 ggca
//
LOCUS       DACCYH010000216         1135 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.226, whole genome shotgun sequence.
ACCESSION   DACCYH010000216 DACCYH010000000
VERSION     DACCYH010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.226"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..859)
                     /locus_tag="IVY85_002980"
     CDS             complement(<1..859)
                     /locus_tag="IVY85_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045925.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTL"
BASE COUNT          363 a          210 c          117 g          445 t
ORIGIN      
        1 ctaatgttcc tataaaatca agtcctctac attttaaagg ttcacttaaa ctattaatat
       61 agttaataat gttgtctatc cttttctctt tatcttcacc ttcaatctgg ggagcatata
      121 gtccaatttc ctcccaataa tatgaatctc tttcaattcc catgatggta gctttggcta
      181 atttcaaatc aaataacgat tttagaattc ttagtgtctc aataaagtct tctctcttca
      241 actctttaat ccattgtgaa ttttctggat tatctaaaat ttcaagttca tcttcattat
      301 aagtatattt caaatttgac ttgcttaact catagataaa tgaagtttct tgactcataa
      361 ttcttttaag tacttgacta taagtttcgt ttgactttgc ccatttcgct aattctacca
      421 ctgtattcac taataaatcg aaataatgat gttctcgttc aatagtctca gcattttgaa
      481 aatgaaaatc acctttataa actccattct tttccactgt tttattaata ttttttaaaa
      541 accagaatga gtttgcaaat ttagtcgtac tttttcgatt aactaagtta ataactcttc
      601 catgtaataa taagacaaaa atacataaat ttttagcttc cttattatta gatactaatt
      661 gatcaatcat agggagccat tttttgattt tcactaattg attgctcata aatctttcac
      721 tggacttttg ttcaatctta aattgcaaaa cttctaactc ataattccca tcttctgatt
      781 tatttttggg aacaactatc aaacttttat aaagtttatc cacatcaaaa tttatctttt
      841 ttgcataatt tgtagccatt aatacatctc cttattcata ataaatttca tatgacgctc
      901 ctctcaaaat ttaccggtag actagaaaaa agaataaaag atacgtcaat tcacacttta
      961 ttcttaaaaa aaaactggtc tctctaccgg taatatcgat tgagtccgcc tttatcgact
     1021 ttatcctttt tttatattaa attttttcac cggtagacag ggagagaaag aataaactaa
     1081 aaagccctta attcttaatg ttttcacctc cttaccgttc ctaccgctaa aattt
//
LOCUS       DACCYH010000217         1104 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.227, whole genome shotgun sequence.
ACCESSION   DACCYH010000217 DACCYH010000000
VERSION     DACCYH010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.227"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..851
                     /locus_tag="IVY85_002981"
     CDS             <1..851
                     /locus_tag="IVY85_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP8045926.1"
                     /translation="LKKESFSIVHNQVYFGGGINLWLAKKNGIRQRIAHSHATEDGKS
                     QNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQNHPFEIVHNGIDLELYSKNSEA
                     KVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGEGSLRN
                     KLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLV
                     LADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQELKSYDVSYTTEQMES
                     IYSRDH"
     gene            884..>1104
                     /locus_tag="IVY85_002982"
     CDS             884..>1104
                     /locus_tag="IVY85_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP8045927.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQA"
BASE COUNT          425 a          124 c          205 g          350 t
ORIGIN      
        1 tactaaaaaa agaatctttt tctatagttc acaatcaggt ttattttggc gggggaatta
       61 atttatggtt agccaaaaag aatggaatca gacagagaat tgctcatagc catgcaactg
      121 aagatggaaa atctcaaaat attgtaatga atgtattgcg taaattttta accaaattgt
      181 tgttacaaaa tgcaacagac taccttgccg ttagtcaaga agctggtgaa agtctgtttc
      241 aaaaccaccc ctttgagata gttcataatg gtattgattt agagctttat agtaaaaatt
      301 cagaagctaa ggttaataag agaaaagaat tggatatctc aatgagtact tttgttgtgg
      361 gaaacattgg acgattagag aaacaaaaaa atcaatctta tttgttagag atttttttag
      421 aaatactaaa aatgaattct gattcttact tgattttagt aggtgaaggg tcacttagaa
      481 ataaactgaa agccaaagca gtagaattgg atatagagaa aagagtaaaa tttttaggag
      541 aaagaaacga tattcccgag ttattatctg tgatggatgt ttttgtaatg ccttctcttt
      601 atgaaggtct gccaatggtt ggattagaag cacaagcagc aaaattaaaa ttagtcttag
      661 cagatactat ttccaaagat acaaagttaa caaataatgt ggattttgta tcattagatg
      721 aaacaccaca agaatgggca aaaaaagtaa taaaagagcc gtatgctaat aaaataacac
      781 aagaactaaa gagttatgat gtttcgtaca ctactgaaca aatggagagt atatatagta
      841 gagatcatta aaaatgttca agatatttta ggagtgatta tttttgaaaa tatgttattt
      901 gatattggca tacaatgata ttggcaactt aaagagattg gtcaatcgat tgaacgaaaa
      961 tgcagttttt attattcata tagataaaaa aactgatata gaaccattta ttgaagcatt
     1021 taaatcatat gataatgttg aatttttatg taataaaaac cgtgttaaaa tttattgggg
     1081 tggtttttcc attgtacaag caga
//
LOCUS       DACCYH010000218         1079 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.228, whole genome shotgun sequence.
ACCESSION   DACCYH010000218 DACCYH010000000
VERSION     DACCYH010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.228"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            250..>1079
                     /locus_tag="IVY85_002983"
     CDS             250..>1079
                     /locus_tag="IVY85_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8045928.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          379 a          152 c          183 g          365 t
ORIGIN      
        1 ataagttatt cttttattac tatgtttggg ttttatagca aagaaagttt aataagagtt
       61 tggacctagc aaacaatatt aatgaggtca ttaaatctta ttatctttag tgatataacg
      121 aaatatctac acagacaaaa tgatagcgta aaatattttg tgtaaataga tatttagggt
      181 agaaaaaaag aaagtccatt ctgtaaaatt aaagttacca caaccgaaat tttataggag
      241 gactttctca tgcccaattt tactacagaa attatggaaa cactaatcaa taaaggtgat
      301 ttagatgaat tatttcgatg tcatttagaa ttagctgtta attcattatt gcaggctgaa
      361 ttaacagctt ttcttgatta tgaaaaatat gatcgtgctg ggttcaattc aggtaattcc
      421 cgtaatggga attattcacg ttcattcaga acagagtatg gagaattaaa tttggtaatt
      481 cctagagata gaaatggtaa gttttctcaa caaactttac ctgcttataa aagaaccaat
      541 gactctttag aaaccactat tattcagcta tttcaaaaag gaattactat gtctgaaatc
      601 tctgaattga ttgaaaaaat gtacgggcat cactatacgc cacaaaccat ttcaaatatg
      661 actaaaatag tttctgaaga tattattgcc tttaaagaaa ggtccttaga atccagatac
      721 tctgtcattt ttatggatgc tactcacatt cctttaaaaa gacaaactgt ttcaaaagaa
      781 gcagtttata ttgtaattgg cattcgatta gatggaacca aagaggttct tgggtttagt
      841 cttgctccaa ctgaatctgc ttatgtttgg aaagagattc ttcaagacct aaaagaccgt
      901 ggtttaaaag aggttttatt agtcgtaact gatggtttaa gtggcattaa cgatagtatt
      961 catagtgttt atccaaatgc tcaatttcaa caatgttgtg tccatatatc tagaaatatc
     1021 gctcataagg ttcgtgttag tgatcgacaa gaaatctgta gcgatttcaa attagttta
//
LOCUS       DACCYH010000219         1068 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.229, whole genome shotgun sequence.
ACCESSION   DACCYH010000219 DACCYH010000000
VERSION     DACCYH010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.229"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="IVY85_002984"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="IVY85_002984"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            203..276
                     /locus_tag="IVY85_002985"
     tRNA            203..276
                     /locus_tag="IVY85_002985"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Asn,seq:gtt)
     gene            316..405
                     /locus_tag="IVY85_002986"
     tRNA            316..405
                     /locus_tag="IVY85_002986"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Ser,seq:gga)
     gene            420..491
                     /locus_tag="IVY85_002987"
     tRNA            420..491
                     /locus_tag="IVY85_002987"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:453..455,aa:Glu,seq:ttc)
     gene            507..579
                     /locus_tag="IVY85_002988"
     tRNA            507..579
                     /locus_tag="IVY85_002988"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:540..542,aa:Val,seq:tac)
     gene            589..661
                     /locus_tag="IVY85_002989"
     tRNA            589..661
                     /locus_tag="IVY85_002989"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Asp,seq:gtc)
     gene            697..772
                     /locus_tag="IVY85_002990"
     tRNA            697..772
                     /locus_tag="IVY85_002990"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:730..732,aa:Phe,seq:gaa)
     gene            774..854
                     /locus_tag="IVY85_002991"
     tRNA            774..854
                     /locus_tag="IVY85_002991"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:808..810,aa:Tyr,seq:gta)
     gene            861..934
                     /locus_tag="IVY85_002992"
     tRNA            861..934
                     /locus_tag="IVY85_002992"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:893..895,aa:Trp,seq:cca)
     gene            937..1009
                     /locus_tag="IVY85_002993"
     tRNA            937..1009
                     /locus_tag="IVY85_002993"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:970..972,aa:His,seq:gtg)
BASE COUNT          252 a          240 c          288 g          288 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgctgatgtt attccggcat agctcagttg gtagtagcgc atgactgtta
      241 atcatgatgt cgtaggttcg agtcctactg ccggagttct cattttgaga aagagtattg
      301 agcttctttc actctggaga gttgtccgag aggccgaagg agcatgattg gaaatcatgt
      361 agatggtgcc aaactgtctc aagggttcga atcccttact ctccgtagca aatttacctg
      421 gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc
      481 cgtacgggtc attttaattg aatgttggag gtttagctca gctgggagag catctgcctt
      541 acaagcagag ggtcagcggt tcgatcccgt taacctccat aatgaactgg ttccgtggtg
      601 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
      661 gtcgttcttg ttattcaatt aataatttta aaatatggcg cggtagctca gttggtagag
      721 caacggattg aagctccgtg tgtcggcagt tcgattctgt cccgcgccac catggaggag
      781 tagcgaagtg gctaaacgcg acggactgta aatccgttcc ttagggttca gtggttcgaa
      841 tccactctcc tccattttat aggggcatag tttaaaggta gaacagcggt ctccaaaacc
      901 gttagtgtgg gttcaattcc tgctgcccct gccatggcga atgtggcgaa gtggttaacg
      961 caccggattg tggctccggc attcgtgggt tcgattccca tcattcgccc tttttattct
     1021 tggggtatag ccaagcggta aggcaaggga ctttgactcc ctcatgcg
//
LOCUS       DACCYH010000220         1045 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.230, whole genome shotgun sequence.
ACCESSION   DACCYH010000220 DACCYH010000000
VERSION     DACCYH010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.230"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            6..980
                     /locus_tag="IVY85_002994"
     CDS             6..980
                     /locus_tag="IVY85_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP8045929.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          376 a          132 c          182 g          355 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaac
//
LOCUS       DACCYH010000221         1035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.231, whole genome shotgun sequence.
ACCESSION   DACCYH010000221 DACCYH010000000
VERSION     DACCYH010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.231"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..>1035)
                     /locus_tag="IVY85_002995"
     rRNA            complement(<1..>1035)
                     /locus_tag="IVY85_002995"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACCYH010000222         1012 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.232, whole genome shotgun sequence.
ACCESSION   DACCYH010000222 DACCYH010000000
VERSION     DACCYH010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.232"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..>1012)
                     /locus_tag="IVY85_002996"
     CDS             complement(<1..>1012)
                     /locus_tag="IVY85_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8045930.1"
                     /translation="GKLDDSPSYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKR
                     TRFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVT
                     RILREATKHYQSRMNFLPTVLCMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLY
                     KLTQYFLHFPRKARLKVKYLVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQ
                     LRVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIV
                     DELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQ
                     SGI"
BASE COUNT          288 a          192 c          219 g          313 t
ORIGIN      
        1 aaatacccga ttggtagcaa tggaagatcg tgaatttctt tttaaacgac tgaggtaacg
       61 tagatgaaat tgttttaagg gtttcttgaa agccttccgt acctcgttta acgaaataat
      121 agcgtaataa ttgacaaaaa tgatacgcag ttcgcaattc ttcgttgtag cttaacagct
      181 catcaacgat ttctgtttga ctaattggtc gtttaaacag tcgatgataa tgccgtttga
      241 gtggctccca tgtcgtggaa tctttgagta agagtttcca atatcgcttg agccgacggt
      301 acttcttctg attttcagag tgtgaggttc gaaaacgatt catgatctgg acacgtaatt
      361 gattgaagct gcgattgatg tgttggacaa tatgaaagcg gtcagttacg atctccgcat
      421 ttggaaagac tgttttaagc aactgacaat agctggcatt catgtccatc actaggtact
      481 tgacctttaa acgggctttt ctaggaaagt gcaggaagta ttgagttagt ttgtaaagtc
      541 tgcgatcttc aaggatcgta aatagctgat gggttgtccc atcaacacaa ataaaactca
      601 tggaaccaga aacagacttc attgatttga actcatccat acacaacacc gtcggaagga
      661 agttcatacg tgactggtaa tgcttcgttg cttcacgcaa gatccgtgtc accgtcccat
      721 ccgagacgaa gtacttctta gcgattagtt tacgtgattg attttcgtac agttccatga
      781 gaattgcctg cttcagttcc tttgttagtt gatgatgttc atcaactaag tccgttttcg
      841 cagaaaaggt ccttccacaa tcatggcaga gaaaacgggt acgttttaat tctaaaatag
      901 ttaacttttc tttgactttc aatatctgag tgcgtgtcgt ataggaaccg ttgcggataa
      961 tacgaaccga tcgacagtgt tcacaatagg aaggagagtc atccaatttt cc
//
LOCUS       DACCYH010000223         1003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.233, whole genome shotgun sequence.
ACCESSION   DACCYH010000223 DACCYH010000000
VERSION     DACCYH010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.233"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            370..1003
                     /locus_tag="IVY85_002997"
                     /pseudo
     CDS             370..1003
                     /locus_tag="IVY85_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          184 g          295 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta cct
//
LOCUS       DACCYH010000224          985 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.234, whole genome shotgun sequence.
ACCESSION   DACCYH010000224 DACCYH010000000
VERSION     DACCYH010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.234"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            91..540
                     /locus_tag="IVY85_002998"
     CDS             91..540
                     /locus_tag="IVY85_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045931.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            540..>985
                     /locus_tag="IVY85_002999"
     CDS             540..>985
                     /locus_tag="IVY85_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045932.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRR"
BASE COUNT          358 a          156 c          222 g          249 t
ORIGIN      
        1 aaattccaag aagctgtaaa tcaagcagaa aattacacaa acgaaagaat tccaaatttt
       61 acttttgaaa aaattgggga ggtatagaga atgacagata tcgtaaaaat aaaacaaagc
      121 aatgtacagg tttatccaca aactcattgg aatgctatag aaggcaaacc aacaacggtg
      181 aagggagata aaggcgatcc tggccaagca gcaacaatta ccataggaac cgtttctagt
      241 ggttcaaccg cttctgttac gaatgtagga acatcatctg cagctagatt taattttgtt
      301 ttaccaaagg gagataaagg tgatcctgga ataaatgcca caactacagc tgtagctaca
      361 actacagcaa atgggttgat gtcgtccaca gataaaacta agttggatgg aattgcagct
      421 ggagcacaga aaaatccagg taatgctaca actacgacag caggtttaat gtcagcaacc
      481 gataaagtga aacttgatgg attagcgaat attacatttg agaaagtggg gacggtttaa
      541 tggctgatat tgttcaatta aaagaagacg gagttgctaa atacttaaaa acgcatgtag
      601 aagctattga tggtaaagaa gcattggtgc agacagatgg agaccaagcc attgcaggac
      661 ataagaattt ttcaggttct gtaacaatca ataataaacg tgttttgacg actgatgata
      721 acagatatga agtggtaaat ctggttgtca ctaacggtaa tacagggaca gcaaagcttt
      781 atcgtgaagg aaaaacagtt tctatatatt ttgtggcttt aaacggaaaa agtagtggcg
      841 ggaatgattc ggttatttta actgttccag aaggctatcg gacaccaatt agttttgaac
      901 aactggttgg gtcaatagac cgttctactt tgaacagtgc tcaactatct attggcgcag
      961 acggagccat taaatggaga agaaa
//
LOCUS       DACCYH010000225          962 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.235, whole genome shotgun sequence.
ACCESSION   DACCYH010000225 DACCYH010000000
VERSION     DACCYH010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.235"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(13..86)
                     /locus_tag="IVY85_003000"
     tRNA            complement(13..86)
                     /locus_tag="IVY85_003000"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50..52),aa:Ile,seq:cat)
     gene            complement(641..955)
                     /locus_tag="IVY85_003001"
     CDS             complement(641..955)
                     /locus_tag="IVY85_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045933.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          354 a          149 c          152 g          307 t
ORIGIN      
        1 tggctacatt aatgtaccct gtaggactcg aacctacgac cggacggtta tgagccgtct
       61 gctctgacca actgagctaa gggtagtggt tgttgccaca taaagccata aacaatcaac
      121 cagtagaatg cgtggcaaca aacctgttat cgcatatctt ggagtgtgac tatttatgag
      181 tgatagtgaa gatatgcgtc tccatatatg tatggttgag acagataaac atgtatgaaa
      241 aacgttgtta aatcaacgtt tttttatttt ggtcaaggag agaaaagtag agaaatacaa
      301 aattttttgc attgattttg cattcaaaaa aacattcaaa catttagaaa tccccaatac
      361 gtattcagga aaatctattt taaggtttac aaaaagaact tttgttcgta cactcttttt
      421 gaggtgaact tttatgctga tggaaggaaa aacacaatta tggtttaagc atgttccttc
      481 aaataagttt tttaattata aactctagag aaattaattc atcctaacat aaaataattg
      541 ttcactcaaa agtttttcta gaacaattaa aacagcaggc ttcacattcg ctatacattt
      601 gtaagaagta ataattcctg tactaaaagg cataattgct ttaattattt tttgacttct
      661 cttcaaacac aagaagtagt tgtgtaataa ttgcagaaat aaggattcca ataatagata
      721 taaaatttga catggaaacg tctataacat taaaagaata aagtgttgaa gaaagtaaac
      781 tgattgaaat aacccctccg taaaaaatag tctgtgaata tacccacttt ttttcagaag
      841 acgtacttcg tttggttcta aaacctatcc ctgcatttct actcttatca aaaaaccaaa
      901 tatttagaag taataatatt gctattatta agataaaatt actgtaaaaa tccaaaagct
      961 at
//
LOCUS       DACCYH010000226          943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.236, whole genome shotgun sequence.
ACCESSION   DACCYH010000226 DACCYH010000000
VERSION     DACCYH010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.236"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..884
                     /locus_tag="IVY85_003002"
     CDS             <1..884
                     /locus_tag="IVY85_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8045934.1"
                     /translation="CKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQ
                     RILVNILARLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKE
                     KILYSLAFARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQ
                     HLLPVILEKENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSN
                     ESIKIQNDFMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFEL
                     KEEALMDQSKKENQVTS"
BASE COUNT          346 a          161 c          151 g          285 t
ORIGIN      
        1 tatgtaaaga gaattttcct gcaacttctg tgaaagacgt acaaaaagaa ttcacagagt
       61 atgaatatat tgactttttt attgatccct ctgtagatat tttgcgacct aatctgccaa
      121 tttgtcgcga acaacgcatc ttagtgaata ttttagctcg actttctatc acgaaaatcg
      181 aatcggaaga aacaagcttg gagcaataca aacttttaaa aaaagcaaac gttcctcatg
      241 ttctttattc aagagaaatg gtggagtcct tttgtactgt atttgatttg tttatacctg
      301 aaaaagaaaa gattctttac tcattggcat tcgcacgaaa taaactttat aatggatttc
      361 ttaacttcaa tcaaccaaat acgccccttc ctactttcat gctttatcaa gaacatagcg
      421 tgttgaaaga agtccaaacc gccattgaac aattttatat ggatttcaga gaacaaaatc
      481 aacacttgtt accagttatt ttagaaaagg aaaatgtgca atggatggtt gaagacctag
      541 tacatttgta tgatcgctac aaaaaacagc cgcgaattgt gattggtgtg aactatacgc
      601 gtgattttta tatcagtaaa gatctgatcg ttaagataga acaaattttt tcaaatgaat
      661 cgatcaaaat acaaaatgat tttatggaga actgcaacat cgttatcagc gactgccctt
      721 tgaaacaatt gccttctcat atcaaaaaaa tctatatttt aaaaggaatc atcacaccag
      781 aagactggaa aaaagtgatt atgaggatct cagaatatat ctttgaatta aaagaagaag
      841 cactcatgga tcaatcgaaa aaagaaaatc aagttacttc ctagaccaaa taagatacat
      901 aaatccgaat gcttattaac aacctccgga tttatgtatc aag
//
LOCUS       DACCYH010000227          936 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.237, whole genome shotgun sequence.
ACCESSION   DACCYH010000227 DACCYH010000000
VERSION     DACCYH010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.237"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            81..314
                     /locus_tag="IVY85_003003"
     CDS             81..314
                     /locus_tag="IVY85_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP8045935.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            311..718
                     /locus_tag="IVY85_003004"
     CDS             311..718
                     /locus_tag="IVY85_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP8045936.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT          349 a          134 c          159 g          294 t
ORIGIN      
        1 agacatgaga gggactatgc ttgatttaaa atcaacaagc gtatatactg tatatacaaa
       61 gaaaaaggag ggagaagact atggcaatta aagaaagaaa atttagggaa gtggggaact
      121 cagtagtatt aactatttca aaggagtttc ttaaagaaaa tgggttaaat cccggagata
      181 cggttttttt agatgaagaa aaacttcgag cggcaattac aaaaaaacaa gccttatctg
      241 atgaaattga tttagcaata tctcaagcat taactgaata tgatactact tttagagagc
      301 ttgtcgataa atgagtattc attacctaga agccaaagat attataaaaa tgaatgtaat
      361 ccaaataaaa aaatactctc ctaatgaacc ccttggtgta aaggatgcga atgctttaga
      421 aatgtgtgta gggcaacttc gagcctcagc ttttggtgaa gaggtttatc cttcgattta
      481 tgaaaaagca gctatcctac ttatacaatt gataaagaaa catccttttc acaatgcaaa
      541 taaacgtact gctttcttag ctacttttgt ctttttgaaa atcaatggac agcttttagc
      601 tattgatcaa aaagatgctg taaatttagc ggtatatatt gctacgtatg ataaggattt
      661 cgatcaacta aaatatgaag tgatcaacac tataaagaca catacgcagc ccatttaagg
      721 agttactgta tgtgtcttta tagggttatt agctgctata gcgaaataat tgaagtaaat
      781 tacaaatagc tataattcaa atccaactat atagaaaacg taaattttct atatagttgg
      841 attaagataa cgactattta ttttatcata tttttactat accctatact tttacttggt
      901 atatttatga ataatcgcaa atttacccct atacta
//
LOCUS       DACCYH010000228          892 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.238, whole genome shotgun sequence.
ACCESSION   DACCYH010000228 DACCYH010000000
VERSION     DACCYH010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.238"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            82..>892
                     /locus_tag="IVY85_003005"
     CDS             82..>892
                     /locus_tag="IVY85_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8045937.1"
                     /translation="MEIFGFYDTAFHINRALSLESIFSSPINFETFRSYGMQVNNFYP
                     WLTLYPLFLLIKFTNLAIGYNLFLYIVTLITLFICHYVMYEITKKHVTSSFFAIIYTT
                     SSFRSVEIFLRGAMGELLAMSILPLILLGFIKLYDSKKESWVMLAISMTLLIYTHVLS
                     VAMTMVMIIIALIIQFYRKKKIEIFLIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLN
                     RPYVDILQKRAIYLGKEFLIGSIDSELLSFGIGLTLLICLIILTFNFKKLGI"
BASE COUNT          284 a          118 c          126 g          364 t
ORIGIN      
        1 ttgaaaaaaa ttttttctgt aaaaatattt atagtttatg catttgttag tttattatat
       61 ttatttccag catttaaaaa catggaaata tttggctttt atgatacggc atttcacatt
      121 aatcgtgcgt taagtttaga atctattttt agctccccta ttaattttga aacgtttaga
      181 tcatatggca tgcaagtaaa taatttttac ccatggttaa ctctatatcc tttattttta
      241 ttgatcaagt ttactaattt agcaataggc tataaccttt ttctttatat tgttacgctc
      301 attactctat ttatatgtca ttatgtaatg tatgaaatta ccaaaaaaca tgttactagc
      361 tcctttttcg ctattatata tacaacatct tcttttagga gtgtagaaat atttcttcga
      421 ggggcaatgg gggaattact cgcaatgtca atacttcctc ttattttact cgggtttata
      481 aagctttacg actcaaaaaa agaatcttgg gttatgttgg cgattagcat gactttatta
      541 atttacactc atgttctttc tgtagctatg actatggtaa tgattataat tgctttaatt
      601 attcaatttt atcggaaaaa gaaaatagaa atcttcttaa ttattaaatt aataaaagct
      661 gctatagtta ctttacttct ttcactagcg tttataggtc ctatgattga gcagacattg
      721 tatcaagatc tgaatagacc ttatgtggat atattacaaa aaagagcaat ttatctgggt
      781 aaagagtttt tgattggaag tatagattca gaattactat catttggtat tggcttaact
      841 ttgcttattt gtttaattat actaacattt aactttaaaa aattaggaat ct
//
LOCUS       DACCYH010000229          871 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.239, whole genome shotgun sequence.
ACCESSION   DACCYH010000229 DACCYH010000000
VERSION     DACCYH010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.239"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(62..262)
                     /locus_tag="IVY85_003006"
     CDS             complement(62..262)
                     /locus_tag="IVY85_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8045938.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          282 a          149 c          136 g          304 t
ORIGIN      
        1 aattttttcg tacaaaaaaa ccgtcttttc tttcgaaaag acggttaaaa gtcaattttt
       61 attaagcttt aacaatgttt gttgcttgag ggccacgttg accatcttca acatcgtaag
      121 acactgcttg gccttcttct aaagttttga agccgtcacc ttggatagct gaaaaatgtg
      181 caaatacatc atttccatct tcaccagtga taaagccaaa acctttgtct gcgttgaacc
      241 attttactgt accgttattc ataaataaat tcctcctgat acgtatcaat gtacgttttt
      301 tcgttgcaat aaatatttga taggcaaaag gagttttatc tatgtagata tatcgctcaa
      361 attacgtttc aaaatagatt actcatttaa tatatcatgc tttttctaat aacacaagga
      421 taatgcaatg aaatataaaa ttcattgtct tctattgtct tcgaatcgaa gaaaaagttg
      481 aataaccgcg attgattcac tttttgtttt cttgtttatt tgcttttatc tgcaactttt
      541 gactttaaaa tttcttaact atgaaaacct ttcaattggt ggtatactta taaatggaat
      601 ttcgttatca acgcgttcgt tgcctttcag caagtattta aagaaaggag aatggctatg
      661 acattctata acaaagatat acaatatcga gtaaccttgg atacagattt gaatctattt
      721 gtcgtttttg acaaagaaaa taccaatcat gtagcgacag gtgttacgat cgaacaagca
      781 gtgcaagaat taaaaaaatc tgcataagga gtggaaaccc tcatatggac gattctcgtt
      841 cttctcttta aacgcatcta ctggtagata a
//
LOCUS       DACCYH010000230          857 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.240, whole genome shotgun sequence.
ACCESSION   DACCYH010000230 DACCYH010000000
VERSION     DACCYH010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.240"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            75..689
                     /locus_tag="IVY85_003007"
                     /pseudo
     CDS             75..689
                     /locus_tag="IVY85_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          315 a          136 c          152 g          254 t
ORIGIN      
        1 aacattgaaa aatttagagt tctaacgtat gattagggtg tattataaac gttatagaaa
       61 agtagggaat aaatttgaaa attgcttata ttagagtttc atccatcgat caaaatgaac
      121 aacgtcaaat tgaagaaatg aaaaagtttg gcgctgaaaa aatttttatc gaaaaacaat
      181 ctggagcgac gattagtcat cggccgattt tccaggaagc tttagatttt gtcagagagc
      241 aagatatttt tattgtagaa gccattgatc gacttggacg caattatgac gagattatcg
      301 attcagtgaa ttatctaaag aagaagaatg tgcgacttat tatcaccagt ttaccgatca
      361 tggctgaagc aattggaaat cctctcctag ataaattcat caaagactta atcatttaaa
      421 tctcagccat gattgctgaa caagaacgta ctgaatcgaa acgacgccag gcacaaggaa
      481 ttaaaatcgc taaagcaaac ggcgtctata aaggtcgtcc taagttatac agtgcagaca
      541 ccaaagatcc tcaacgtcgc cttgtctata ggagtatcgt tcaggatctt gagaatgggg
      601 ttgctatctc aaagattgca acagactaca atgtgactag gcaaacaatt taccgaataa
      661 aaaaagaaat cgaccaacta attgtatgat ttcttttttg aatcacgtaa ttattaatta
      721 gctttgtcat caatacagac aatcatttta gattcatcta gctaagttga ttacatttat
      781 gttaaaaaga atttagttaa cataaatata attgatgcat aaatcgtcct tatttaaagg
      841 catttaacat tgacaac
//
LOCUS       DACCYH010000231          852 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.241, whole genome shotgun sequence.
ACCESSION   DACCYH010000231 DACCYH010000000
VERSION     DACCYH010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.241"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..116
                     /locus_tag="IVY85_003008"
     CDS             <1..116
                     /locus_tag="IVY85_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP8045939.1"
                     /translation="KILIIDDVYTTGQTLFHAADCLQNAQPKIIRTISLAR"
     gene            244..795
                     /gene="raiA"
                     /locus_tag="IVY85_003009"
     CDS             244..795
                     /gene="raiA"
                     /locus_tag="IVY85_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP8045940.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT          306 a          151 c          177 g          218 t
ORIGIN      
        1 aaaaaatatt gattattgac gatgtctata caacaggaca gacgcttttt catgcagcag
       61 attgcttgca gaacgcacag ccgaaaatca tccgaaccat ttctttggca agatgaatca
      121 gatgtggaaa tttgatagcg atttaatttg caaataacct agagttcgat ataatagaat
      181 taagaagagg gaaagagtgg tccttctcct tcttgattgt ggtagacgaa aggggtaatt
      241 tttatgttta gatataatgt acgcggcgaa aatatcgaag taactgaagc gattcgcgac
      301 tacgttgaga aaaaagtagg caaactagaa cgctatttca gtgacgcacc cgatgcaacg
      361 gcgcatgtga acttaaaagt ttatacagaa aaaacagcaa aagtggaagt aacgatccca
      421 ttaccttatc ttgtattacg tgccgaagaa acttctccag atttatacgc aagtatcgat
      481 ttggttgttg acaaattgga acgccaaatc cgcaaattca aaacaaaaat taatcgtaaa
      541 tcccgtgaaa cgggcatgaa tactgcagat gctgctgctt tattcaatga agaggacgaa
      601 aacgaaaatg attctgaatt agatatcgta cgtacgaaac gcctttcatt gaaaccaatg
      661 gatagtgaag aagcagtctt acaaatgaac atgctgggac ataacttctt tatttttgaa
      721 gatgcagaaa caaatggtac aagtatcgtt taccgccgta aagatggaaa atacggactg
      781 attgaaacag actgatcagc cttacttaaa taacaacgaa aacttgcagc agctactgac
      841 agctgccgca ag
//
LOCUS       DACCYH010000232          849 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.242, whole genome shotgun sequence.
ACCESSION   DACCYH010000232 DACCYH010000000
VERSION     DACCYH010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.242"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..720
                     /locus_tag="IVY85_003010"
     CDS             <1..720
                     /locus_tag="IVY85_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8045941.1"
                     /translation="GKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFT
                     NRLGTNKRGRLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVY
                     LANCYTLEGRKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITL
                     EEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNK
                     VQLIRLNKYRVLAGNQELNLI"
BASE COUNT          326 a          133 c          167 g          223 t
ORIGIN      
        1 gggaaaacgg aattagaaga aacaagactt tataattcaa tggtattagg tatgcaaaat
       61 tattatcaga ttgcaacgaa tgtaaatctg gattgtatga aactgaatag agctgtaatg
      121 gctatcttca ctaacagatt aggtacaaac aaaaggggca gattgagaaa aacaggcaga
      181 caacttacga caaaggaggc aacaagatat ggtaaatcta aaatgcttcg gtatgttgct
      241 ggcgagggcg aacctatttt ccctgttgga tatatccaac acagaaaacc aatggcaaag
      301 gtgtatttag cgaattgcta cactctagag ggaagaaaat ttatccacac caatctaagt
      361 gttgataaat tactgatgta ccagttaatg aaattatctt tggaaaatcg cacaatagaa
      421 tacgcagaca atcgaatttc gctattttct gctcaacatg gaaaatgtgc tataactctg
      481 gaagaatttc aacaagctaa tgaaatacat tgtcatcata ttgtaccaac tggagctggt
      541 ggaaatgacg attataaaaa tcttattttg gtaaaagagg cggtacaccg tctaatacac
      601 gctaaaacag aagaaacaat acaaaaatat atagctctat tgaagctaaa taaagtccag
      661 ctcataagac taaataaata tagagtatta gctggcaatc aagaattaaa cttgatatag
      721 gtctactaca taaatacttt tcaatataaa cacgatggaa cgcggagtgc ggtgaaagtc
      781 gcacgctccg tgtgaatggg gggaaaagtt tgagatattt ataatatcag gaacttacct
      841 atccatata
//
LOCUS       DACCYH010000233          842 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.243, whole genome shotgun sequence.
ACCESSION   DACCYH010000233 DACCYH010000000
VERSION     DACCYH010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.243"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>842
                     /locus_tag="IVY85_003011"
     CDS             <1..>842
                     /locus_tag="IVY85_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP8045942.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLK"
BASE COUNT          367 a          108 c          136 g          231 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 tt
//
LOCUS       DACCYH010000234          756 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.244, whole genome shotgun sequence.
ACCESSION   DACCYH010000234 DACCYH010000000
VERSION     DACCYH010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.244"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            17..247
                     /locus_tag="IVY85_003012"
     CDS             17..247
                     /locus_tag="IVY85_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045943.1"
                     /translation="MGFRTKRSLSSKKNWVYSQTIFYGGIVLISLLSSTLYSLNIIDV
                     STSNSISIIGIIIAAIITQLFLVFGEKKRSKK"
     gene            complement(338..703)
                     /locus_tag="IVY85_003013"
     CDS             complement(338..703)
                     /locus_tag="IVY85_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045944.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          285 a           98 c          135 g          238 t
ORIGIN      
        1 gagtagaaat tcaagtatag gttttagaac taaacgaagt ttgtcttcta aaaaaaattg
       61 ggtatactca caaactattt tttatggagg aattgtttta attagtttac tttcttcaac
      121 actttattct ttgaatatta tagatgtttc cacctcaaat tcaatatcta ttattggaat
      181 cattattgct gcaattatta cacaactgtt tttagtgttt ggagagaaga aaagaagtaa
      241 aaaatagaaa aactagtgat agttttggtg atataagaaa aacatatatg tataaaaaga
      301 agaaagtagc ttctctttgt acatatatgg tttgttttta tggatgccga gcttttttat
      361 aagataaggc taccgcccat aacgacccaa ggatttgtgg aactaagtaa atacctgcaa
      421 gaagcaagat cagctgggtc ggttttaaag gatgatagaa gtaacgccat tcagtaaaat
      481 tggcgcaaaa taaataggaa gtgacagtag atataatatt ggcaatgaat aaagatataa
      541 tacgcctgtt cctttgaaaa taatatccag caaaaagaga taaaaatatc aatgtgatga
      601 acccgcttac ggaatgataa cgaaaatcaa gcagtaaggc gaaatgcaca taaggaacaa
      661 aagataaaag caacggaagg tttttccagc aagtttttat catctttcat caccacctaa
      721 caagtgttat tattttgtta gttttattgt aaattg
//
LOCUS       DACCYH010000235          754 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.245, whole genome shotgun sequence.
ACCESSION   DACCYH010000235 DACCYH010000000
VERSION     DACCYH010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.245"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..222
                     /locus_tag="IVY85_003014"
     CDS             <1..222
                     /locus_tag="IVY85_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8045945.1"
                     /translation="TEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIR
                     GLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            403..>754
                     /locus_tag="IVY85_003015"
     CDS             403..>754
                     /locus_tag="IVY85_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8045946.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIF"
BASE COUNT          278 a          134 c          135 g          207 t
ORIGIN      
        1 acagaacatc gtaccattaa atacctgaat aatttgattg aacaagacca tcgtccagta
       61 aagagacgca ataaattcta tcgaagttta cgcactgcct ctaccacgat taaaggcatg
      121 gaagccattc gaggattata taagaaaacc cgaaaagaag gcactctctt cgggttttcg
      181 gtctgtactg aaatcaaggt attattggga atcccagctt aaatcataga taccgtaagg
      241 gattttattc tttatttaaa actttgcaac agaaccaccc gcactatgcc attacattta
      301 tatctacgcg aattgtaaaa ttaagttaga aaaataaaaa ggcatttatg gtacactcaa
      361 attgtatttc cgacgaaaga aaacaaagga gtgtaaccat aaatgaccta caaacatctt
      421 accatagacg aactgacaat gatagaatca tattatcttc aacataataa gccggttgaa
      481 atcgctaacc gaatgggacg tgctatacaa actatttata atgtagtcaa taagttcaag
      541 caaggcaaga ctgctcttga ttattggcac cagtataaag aaaataagaa aaaatgtggt
      601 agaaaagtca ttcaattacc tgctcatgaa gtagattaca ttaaagagaa agtcactctt
      661 ggttggacgc ctgacgtcat tatcgggcga aaagaaaggt ctgtttcatg cggtatgaga
      721 acactttatc gtttattttc taaaggaata tttg
//
LOCUS       DACCYH010000236          750 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.246, whole genome shotgun sequence.
ACCESSION   DACCYH010000236 DACCYH010000000
VERSION     DACCYH010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.246"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            2..>750
                     /locus_tag="IVY85_003016"
     rRNA            2..>750
                     /locus_tag="IVY85_003016"
                     /product="23S ribosomal RNA"
BASE COUNT          232 a          146 c          224 g          148 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag
//
LOCUS       DACCYH010000237          742 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.247, whole genome shotgun sequence.
ACCESSION   DACCYH010000237 DACCYH010000000
VERSION     DACCYH010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.247"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            49..285
                     /locus_tag="IVY85_003017"
     CDS             49..285
                     /locus_tag="IVY85_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8045947.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            282..680
                     /locus_tag="IVY85_003018"
     CDS             282..680
                     /locus_tag="IVY85_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP8045948.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT          266 a          116 c          133 g          227 t
ORIGIN      
        1 tataatgtac ttactataga atgagaatgg tatagagggt ggcgaaatat gaaaaaaatg
       61 aaaaagtata aaattagaaa atctggcaat agcgatgtaa caactatccc accagaagta
      121 aaagaattca tgggtgtgca gacaggtgac gcaatcagtt atgtttttca atctgatggt
      181 tctgtacgaa tgatcaaagc acaagaagaa cctgatattg attcattagt tgattcgatc
      241 atgaatcaat atgaagacgc gttaaaagat ttggttgatt tatgaggtat ctttcgacta
      301 aagaaattat caaaatcaac gcgaaagtta ttttacgtca ctcgccaggc gaaatgatcg
      361 gtgtaaaaga tgcaaatgca ttagatatgg ccgtcaaaca gccttcacaa gcagtattca
      421 atcaagaact ctatcctgaa atctatgaaa aagcggcgat tttagcaatt aatttagcta
      481 aaaaacatcc ttttcataat ggcaataaac gaactgctct tgttgccatg ttgttgtttc
      541 ttcaattgaa taattgtcca gttgcttttt ctcgtcaaga agctgttgat tttatcataa
      601 tgattaccac tagttcattg gatttcgatt cactaaagtc caagataacc agttatttac
      661 gacaaaccgc aataaaatga acgaattaaa aactttattt ctcaattaga tcagcatgtc
      721 attaaggaat atgctgattt tt
//
LOCUS       DACCYH010000238          712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.248, whole genome shotgun sequence.
ACCESSION   DACCYH010000238 DACCYH010000000
VERSION     DACCYH010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.248"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            72..>712
                     /locus_tag="IVY85_003019"
     CDS             72..>712
                     /locus_tag="IVY85_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8045949.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSG"
BASE COUNT          249 a          109 c          106 g          248 t
ORIGIN      
        1 ttgtctaaat ttatgaaaaa aacagtaata ttttcaagtt ttaacaattt aagtaaaaag
       61 gagcctcttt catgcaaact caagaagcga ttaaaacgtt tctcttgaag aaaaaaagca
      121 atacaaaatt agatattttt cttttcctat ctgaacatcg tttctttatt actacacaat
      181 atttagcaga tcatttccat atgtctgaat caaatttttt actatacatc aaagaattag
      241 aacaagactt tgagcgtctc aatctaacag aactgcatat tgataagcaa aaaccttttt
      301 taaagttgaa ttttgaagga attgatccgg cttactgcta ttatcgtcta tttggaagat
      361 actgcaacga atcagtcagt tatcagatct tgacttcttt gttcagctgt cagacaaatt
      421 cgattatcag tttaagtcag caaacaaact atagtgcctc ttatttgtat acgaaaatga
      481 aaaagattaa tgcattttta gcattgtacg gtttgtctat ctccttttct aataatggca
      541 gaaagtcttt ttctggaaaa gaggtacaaa ttcaatatgc agcattagat atttattgga
      601 acatcttttc aagtactgct acagaatttt atgatgaaga tccgcaagta gtggctttta
      661 tgatgaatac tttttttaaa aaagaagtac ttgaccgact aaatagcggt at
//
LOCUS       DACCYH010000239          668 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.249, whole genome shotgun sequence.
ACCESSION   DACCYH010000239 DACCYH010000000
VERSION     DACCYH010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.249"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(1..526)
                     /locus_tag="IVY85_003020"
                     /pseudo
     CDS             complement(1..526)
                     /locus_tag="IVY85_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177294.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
BASE COUNT          218 a          108 c           68 g          274 t
ORIGIN      
        1 tcaacgatat tcttgtaagt aattatgccc caactaggat ttgtgggttc atttccatct
       61 ggagttaaaa aaacaaaaat ttttttatag ttagaataat tgcgttctat atatatttgg
      121 tatttattaa gttgccctcc agactccgaa gacagaattt tattttcgat gcacaccact
      181 attttatttt tttccgatac caataaaata tcaatattct tattttctct gtaaacaatt
      241 aaatcttcat agctattaag taaagcatca aaataattga tagcagcttt ggtatcatca
      301 ttagaatcta tatacctttt caaaaacatt ttaaaaaact catcagataa attgtgggtt
      361 tcatttggag caaacaaaaa agctaaaaaa ttactatgtc ttatttcatt tttagatagc
      421 ttaagtatct gaaatatatt tgttttccca gtccatttag atagttttct tcaatatcat
      481 ccatatcctc taatagattt tttaaacact ttttttcatc tatcatgttt tttctccttg
      541 ccattattct aactaaaatt ttacgtattt gtttctatgt ctacattatc tttttaacag
      601 tatctatttt tcttttgcaa cttacttggc taccaaagca aataaaaaag ttatttataa
      661 atttctcc
//
LOCUS       DACCYH010000240          632 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.250, whole genome shotgun sequence.
ACCESSION   DACCYH010000240 DACCYH010000000
VERSION     DACCYH010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.250"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..343
                     /locus_tag="IVY85_003021"
     CDS             <1..343
                     /locus_tag="IVY85_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8045950.1"
                     /translation="IETDLGNKTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHV
                     RLHKGICTAAFRANNLSCMIYGIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGL
                     PHNLEHLVLQH"
     gene            434..>632
                     /locus_tag="IVY85_003022"
     CDS             434..>632
                     /locus_tag="IVY85_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045951.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLK"
BASE COUNT          265 a           80 c           92 g          195 t
ORIGIN      
        1 aattgaaact gatttaggga ataaaacatt aggacaaacg atacacaata aaagatgtta
       61 tgtgatatta attaatcaaa acctatcacc ttctatgaaa agatttgtat tatggcatga
      121 aatagcccac gttagactcc ataaaggtat ttgcacagca gcttttagag caaacaacct
      181 tagttgtatg atttatggta tagaagctga agctaatatg tttgcaattg aaatgttaac
      241 tagaacaata gatgaacaag atttatctga tatgagtcag ttccaaatta ttgaatattt
      301 agggcttcct cataatcttg aacatttagt attacaacat taaaaataaa aaagcccatg
      361 ctttcacatg gactctttcc tcatttctga gtaaactcta caataattat atcaaagaaa
      421 tgaggagttt tgaatgttat tattgattgg acttatttta ttaattatag gaatcactaa
      481 actgtcgaaa gctaacacaa taaaaaaaga agcttatgaa ttagaacaaa aaaatgatga
      541 aaaactaaat aaattacatg atagagaaag atttatatta agtaaggaag aaacactatc
      601 agatttaaat aaacaaatta ctatgttaaa aa
//
LOCUS       DACCYH010000241          632 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.251, whole genome shotgun sequence.
ACCESSION   DACCYH010000241 DACCYH010000000
VERSION     DACCYH010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.251"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            79..234
                     /locus_tag="IVY85_003023"
                     /pseudo
     CDS             79..234
                     /locus_tag="IVY85_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            253..>632
                     /locus_tag="IVY85_003024"
     CDS             253..>632
                     /locus_tag="IVY85_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8045952.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTK"
BASE COUNT          262 a           62 c          109 g          199 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa ag
//
LOCUS       DACCYH010000242          599 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.252, whole genome shotgun sequence.
ACCESSION   DACCYH010000242 DACCYH010000000
VERSION     DACCYH010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.252"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>599
                     /locus_tag="IVY85_003025"
     CDS             <1..>599
                     /locus_tag="IVY85_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045953.1"
                     /translation="DWLKIEKGNTRTPNISEYKYRGTGMRDSNNPKDYVWDLAPEYVE
                     DNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANATAIDE
                     LDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGKSSGGNDSTILTI
                     PEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRR"
BASE COUNT          216 a          104 c          127 g          152 t
ORIGIN      
        1 gactggctca agatcgaaaa aggcaacaca cgaactccga atattagtga gtataaatat
       61 cgtggtactg gtatgcgtga ttcaaacaat ccaaaagatt atgtttggga tctagcacca
      121 gaatatgtcg aagataactt ggccacagat attaaaattt ctgaaattac tggtaaagca
      181 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      241 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      301 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      361 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      421 tattttgtgg ctttaaacgg aaaaagcagt ggtggaaatg attcaacgat actaacaatt
      481 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      541 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaa
//
LOCUS       DACCYH010000243          575 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.253, whole genome shotgun sequence.
ACCESSION   DACCYH010000243 DACCYH010000000
VERSION     DACCYH010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.253"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
BASE COUNT          186 a           81 c           81 g          227 t
ORIGIN      
        1 tttttcttga tgtgatatcc ttgtcttttt tgtaatgttt ttaacttatt gcttttgcaa
       61 aatttattta cctgttttct ggatcaacga tagtgtacat tatgctatcc ttgtttggat
      121 tttaggacgt ttaaagaggc acttttttac ataacttaat ccatagacct tttaagcatg
      181 ctgtgagata ataataaaga agagtttaaa gcgcacccca aaccacttcc ccataagtgt
      241 attacgcttt aaactctttt atatttgaat tcactaagaa gcataccata tttttgaaaa
      301 aaagtgagaa aaaaggctta taattaggat ttagagtaat taattagatg tttattttat
      361 aggactgata ctatagatta caaatagtat cgtatttcgc aatcttaaaa ttatcttctt
      421 actgtgaata agataataaa atttgtattt gtccttttta ggtaacttat tttaatctat
      481 atacctttct ggatcaacga aagtatactt tatatagtca taacgccgat ggtaactatg
      541 cactgtaacg ataatgctgc taaaatatct tttta
//
LOCUS       DACCYH010000244          569 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.254, whole genome shotgun sequence.
ACCESSION   DACCYH010000244 DACCYH010000000
VERSION     DACCYH010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.254"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            73..>569
                     /locus_tag="IVY85_003026"
     CDS             73..>569
                     /locus_tag="IVY85_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP8045954.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          212 a          105 c           93 g          159 t
ORIGIN      
        1 catacaaaaa aggaagtccc ttctgtagaa taaagttaac gacaaccaat tcacagaaaa
       61 gaggacttcc ctatgaatga ttttactaca gaaattgtgc aaactctagt cactaaaggc
      121 gatttaaatg aattattccg ttcgcactta gaaaaagcga taaacacact cctacggact
      181 gaattaacgg cttttttaga ttacgaaaaa tatgatcgca ctggttttaa ttcaggtaat
      241 tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat atggtgaatt aacattggaa
      301 atacctagag atcgtaatgg tgagtttaaa caacaaactt taccagccta caaaagaaca
      361 aacgatacat tggaaaccac tattatccat ttattcgaaa aaggtgttac gatgtctgaa
      421 attgctgatt tgatcgaaaa aatgtacggt catcactata ctccacaaac catgtccaac
      481 atgactaaag ttctgactga agaagtaaat gcctttaaat ccagagcctt aaatgataag
      541 tatgtcgcta tttttatgga cgctactta
//
LOCUS       DACCYH010000245          565 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.255, whole genome shotgun sequence.
ACCESSION   DACCYH010000245 DACCYH010000000
VERSION     DACCYH010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.255"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(9..203)
                     /locus_tag="IVY85_003027"
     CDS             complement(9..203)
                     /locus_tag="IVY85_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045955.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          196 a          105 c           85 g          179 t
ORIGIN      
        1 gctgtctgct accccggcct aatctggcaa ggggaggagg agatataatg aaataccttt
       61 ttgattttgt ttttgcaatc aagccacttg cagatgtagt gtgcagttac ctctgcaaat
      121 ggcttgattg caaaattgcc aaaaaagaca gtaagcctac ccgttaattc tataatatag
      181 aaacgtaaaa aaaaccccat cagcctgcac gctgatgggg tttggtgata taatgaaata
      241 cctctttgtt actcataatt ataccataat tagtcacgaa tgcaaccaat agaaaaaact
      301 acttagaacg gtagatcatc gctaataaca atgcattgtc caccaaatgg attttgtgaa
      361 gcattaaata cattgttatt aattgtcgct gcttttaact aggcctctcc ccttctatta
      421 taaaagaaga tatatatcat ttcttaatta cctactacct taaaccaaaa tttaagtact
      481 acctaattta agtagtactt aaacaatcta aaaaatagtt atcccaattg ttatgctttt
      541 ataatacgat tatttgtaac aaaat
//
LOCUS       DACCYH010000246          541 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.256, whole genome shotgun sequence.
ACCESSION   DACCYH010000246 DACCYH010000000
VERSION     DACCYH010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.256"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
BASE COUNT          192 a           84 c           86 g          179 t
ORIGIN      
        1 tagttatact tctgtatatt tgaaaagttt tactttgatt tttaaataga aagacatttg
       61 ggttaaattg tgagataata ataaagaaga gtttaaagcg caccccaaac cacttcccca
      121 taagtgtgtt acgctttaaa ctcttttata tttgaagcca ttaaaaagca taccatattt
      181 ttgaaaaaaa gtgagaaaaa aggcttacaa ttggagtggt agttaattag tgacttattt
      241 ttgattttat agcactgata ctataaaata tagatatcat catattacac aatcttaata
      301 ctaacttaaa aaatatctcc tttcacaagt atggtgataa aattcgttcc gggctacctt
      361 tttaggtagc ctactttaat ctttatacct ttctggatca acgaaagtat actttatata
      421 gtcataacgc cgatgatcgc tacgtgcgtc cggcacgtca gtcatgaatc ggctacacta
      481 gactagacag aaaaaataag gtgtgtagaa taataaaaaa cacactggag gatttttcat
      541 g
//
LOCUS       DACCYH010000247          534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.257, whole genome shotgun sequence.
ACCESSION   DACCYH010000247 DACCYH010000000
VERSION     DACCYH010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.257"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>534
                     /locus_tag="IVY85_003028"
     CDS             <1..>534
                     /locus_tag="IVY85_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP8045956.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DACCYH010000248          513 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.258, whole genome shotgun sequence.
ACCESSION   DACCYH010000248 DACCYH010000000
VERSION     DACCYH010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.258"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(107..>513)
                     /locus_tag="IVY85_003029"
     CDS             complement(107..>513)
                     /locus_tag="IVY85_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP8045957.1"
                     /translation="TPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMNA
                     ASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYEDS
                     QITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          162 a          102 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttc
//
LOCUS       DACCYH010000249          462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.259, whole genome shotgun sequence.
ACCESSION   DACCYH010000249 DACCYH010000000
VERSION     DACCYH010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.259"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..430)
                     /locus_tag="IVY85_003030"
     CDS             complement(<1..430)
                     /locus_tag="IVY85_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8045958.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DACCYH010000250          460 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.260, whole genome shotgun sequence.
ACCESSION   DACCYH010000250 DACCYH010000000
VERSION     DACCYH010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.260"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..>460)
                     /locus_tag="IVY85_003031"
     CDS             complement(<1..>460)
                     /locus_tag="IVY85_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAP8045959.1"
                     /translation="DYPRINRNNTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYHGS
                     QWWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPD
                     LPLELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFVRKV"
BASE COUNT          148 a           67 c           62 g          183 t
ORIGIN      
        1 acttttctta caaataattt ttcactttct tttatttgat ctatatccct ttcatcaaac
       61 catttgttca tggaaggatc aataatatga atatttgcta atttcgaagt ttgtaaagta
      121 gtttcttcaa tatttttcaa ttcaagtggt aaatctggtc ctccaataac atttttattt
      181 ttgaacgagg aattaaaaaa aagtgtatga aaaaattttt catctggggc aaacatacct
      241 attttaaaat tcaggtagtc ttgttgattt tgttcataca tttgtattaa ttctgttagg
      301 cattctttcg acaaagccca ccattgagaa ccatgataca aatcaaattt atggtgtcta
      361 atttttgggg gaagtttaat catcctcaga caacgattaa ttatagctct aaaaatttta
      421 aatgcagttg tgttattcct attaattcta ggataatcat
//
LOCUS       DACCYH010000251          441 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.261, whole genome shotgun sequence.
ACCESSION   DACCYH010000251 DACCYH010000000
VERSION     DACCYH010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.261"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..224
                     /locus_tag="IVY85_003032"
     CDS             <1..224
                     /locus_tag="IVY85_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8045960.1"
                     /translation="GNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQSYLI
                     LISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            234..>441
                     /locus_tag="IVY85_003033"
     CDS             234..>441
                     /locus_tag="IVY85_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8045961.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDI"
BASE COUNT          177 a           44 c           75 g          145 t
ORIGIN      
        1 taggaaatgt attactaaaa gtaattgtag atggattcat taactcagag gattcaatga
       61 tttccggtgg aagtttaata aataatagta gttttatgtc aaatataaca acacttggac
      121 aaagttattt aatattaata agtattattg ttttatcagt tgtatttgct tcttcattaa
      181 tgttaatcaa gaagcctaaa gaaatcttat caaaaataag ttaggagaaa ataatggata
      241 tattagaaat aaagaatgta gcatatagtt atgcaaattc taaagaaaaa gttttgtcag
      301 gagtaaatca aaaatttgaa cttgggaagt tttatgcgat agtaggaaag tcaggaacag
      361 gaaaatctac acttctttcc ttacttgctg gacttgataa acctcaaaca ggaaaaatat
      421 tgtttaagaa tgaagatata c
//
LOCUS       DACCYH010000252          431 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.262, whole genome shotgun sequence.
ACCESSION   DACCYH010000252 DACCYH010000000
VERSION     DACCYH010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.262"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..>431
                     /locus_tag="IVY85_003034"
     CDS             <1..>431
                     /locus_tag="IVY85_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8045962.1"
                     /translation="MCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRK
                     IKKALSKRGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRD
                     NPLEAIVTDLTYVRVGKKWNYVCFILDLFNREILGYSCGEH"
BASE COUNT          164 a           74 c           81 g          112 t
ORIGIN      
        1 atgtgcagaa tattaggtct atcacgtcag tcctattatt atcaatcaaa accaaagaaa
       61 gacgaatcag aacttgaaga agtagtcgct gaagaattta tccgcagccg aaaggcctac
      121 ggctcaagaa aaataaaaaa agccttatca aaacgaggca ttcagatcag ccgacgaaaa
      181 attagtagaa tcatgaaaaa tagaggatta aaatcgagct atactgttgc ttattttaaa
      241 gtacatcatt ctacttgcaa tgaagccaaa acgacaaacg tattgaatcg taaattctta
      301 agagacaacc cattagaagc gatcgtaaca gacttgactt atgtacgagt cgggaaaaaa
      361 tggaattatg tctgtttcat tttggatctg ttcaatcgag aaattctcgg ctattcttgt
      421 ggagaacata a
//
LOCUS       DACCYH010000253          429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.263, whole genome shotgun sequence.
ACCESSION   DACCYH010000253 DACCYH010000000
VERSION     DACCYH010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.263"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            151..369
                     /locus_tag="IVY85_003035"
                     /pseudo
     CDS             151..369
                     /locus_tag="IVY85_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          145 a           59 c           75 g          150 t
ORIGIN      
        1 ttaggtagta tatattttta gatacaatat cgatcagttt tgaggaaatg aaattctaga
       61 tattttttgc tacatagagt tactttcaat attttttaac gtaattaggc agaagtcact
      121 catcctctat aggtttgtga tgaaattgcc attgatccat ttaagtaaga gcaaaaaact
      181 ggaggtttat caggtaattg gtctaatgga tttaagtatg cggtcaccgt gtatagagtt
      241 gcttatgaga ttcaagaaaa taaagttatt ccagtattgc tatatggaac acataacaat
      301 ttttatgaat tagccgagaa cactcgcaac tttattcgtg tagatgaatc ggcttcggtt
      361 gagttataac tttagctata tcaatcaacc gaccgacata tgttaaatct atatataata
      421 aaaatacct
//
LOCUS       DACCYH010000254          421 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.264, whole genome shotgun sequence.
ACCESSION   DACCYH010000254 DACCYH010000000
VERSION     DACCYH010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.264"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..390
                     /locus_tag="IVY85_003036"
     CDS             <1..390
                     /locus_tag="IVY85_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8045963.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          163 a           74 c           65 g          119 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caatttattt
      421 a
//
LOCUS       DACCYH010000255          414 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.265, whole genome shotgun sequence.
ACCESSION   DACCYH010000255 DACCYH010000000
VERSION     DACCYH010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.265"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(109..>414)
                     /locus_tag="IVY85_003037"
     CDS             complement(109..>414)
                     /locus_tag="IVY85_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8045964.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          116 a           96 c           82 g          120 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggt
//
LOCUS       DACCYH010000256          350 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.267, whole genome shotgun sequence.
ACCESSION   DACCYH010000256 DACCYH010000000
VERSION     DACCYH010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.267"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(<1..>350)
                     /locus_tag="IVY85_003038"
     CDS             complement(<1..>350)
                     /locus_tag="IVY85_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045965.1"
                     /translation="PYQPNLLDDPYWLGKAPLGENIANKSVTFPIKSSAYSLYQANME
                     EEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRYGNLEPVEGLVDTWGLTFTPQKVG
                     VNYPKRLTIIQYPQS"
BASE COUNT           95 a           68 c           75 g          112 t
ORIGIN      
        1 gattggggat actggataat agttaaccgt ttggggtagt taactccaac cttctgcggt
       61 gtaaaagtca gaccccatgt atcaactaac ccttctactg gctctaagtt accatatcta
      121 taatctccac tatcctcgtt gtaaacaatg aacgtttgga taggtggttt ggttgccttc
      181 atggtaattg tataggtttg acctaaaaca aattcttctt ccatgttagc ttggtatagg
      241 ctgtaggcgc tagatttgat tggaaacgta acagacttat tagcaatatt ctcacccaaa
      301 ggcgctttac ctagccagta agggtcatca agtaagtttg gctgatatgg
//
LOCUS       DACCYH010000257          319 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.denovo.268, whole genome shotgun sequence.
ACCESSION   DACCYH010000257 DACCYH010000000
VERSION     DACCYH010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.denovo.268"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
BASE COUNT          115 a           34 c           59 g          111 t
ORIGIN      
        1 tataagaagg agtataaaaa tgaacaaaca taatagaaaa ataacgaata acaaagaact
       61 gatcttgtta ctaataggga tttggggtat ctttttactc tttaatatgt atatgccgac
      121 tatgagagcg gatgacgtag tttatgcaag tagattagat gaacttggct atttgggtgc
      181 ttcgatagaa cattataaga catggagttc aagaattatt atagaactat tcttaatgtt
      241 cttttctaaa cactttatgc tatggaaatt attaaattct acgattatgt taggaattgt
      301 agtattactt tgcaagtat
//
LOCUS       DACCYH010000258        22020 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.guided.044, whole genome shotgun sequence.
ACCESSION   DACCYH010000258 DACCYH010000000
VERSION     DACCYH010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.guided.044"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            36..1265
                     /gene="rpsA"
                     /locus_tag="IVY85_003039"
     CDS             36..1265
                     /gene="rpsA"
                     /locus_tag="IVY85_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP8045966.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            1447..2757
                     /locus_tag="IVY85_003040"
     CDS             1447..2757
                     /locus_tag="IVY85_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP8045967.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            3000..3275
                     /locus_tag="IVY85_003041"
     CDS             3000..3275
                     /locus_tag="IVY85_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP8045968.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            3510..4556
                     /locus_tag="IVY85_003042"
     CDS             3510..4556
                     /locus_tag="IVY85_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045969.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            4558..5817
                     /locus_tag="IVY85_003043"
     CDS             4558..5817
                     /locus_tag="IVY85_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8045970.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            5823..6404
                     /locus_tag="IVY85_003044"
     CDS             5823..6404
                     /locus_tag="IVY85_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP8045971.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(6486..7361)
                     /locus_tag="IVY85_003045"
     CDS             complement(6486..7361)
                     /locus_tag="IVY85_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8045972.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            7509..7841
                     /locus_tag="IVY85_003046"
     CDS             7509..7841
                     /locus_tag="IVY85_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8045973.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            7953..9161
                     /locus_tag="IVY85_003047"
     CDS             7953..9161
                     /locus_tag="IVY85_003047"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP8045974.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            9315..9770
                     /locus_tag="IVY85_003048"
     CDS             9315..9770
                     /locus_tag="IVY85_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045975.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            10132..10407
                     /locus_tag="IVY85_003049"
     CDS             10132..10407
                     /locus_tag="IVY85_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8045976.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            10420..12498
                     /locus_tag="IVY85_003050"
     CDS             10420..12498
                     /locus_tag="IVY85_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8045977.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            12658..14544
                     /locus_tag="IVY85_003051"
     CDS             12658..14544
                     /locus_tag="IVY85_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8045978.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            14556..15503
                     /locus_tag="IVY85_003052"
     CDS             14556..15503
                     /locus_tag="IVY85_003052"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8045979.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            15523..16050
                     /locus_tag="IVY85_003053"
     CDS             15523..16050
                     /locus_tag="IVY85_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8045980.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(16113..16766)
                     /locus_tag="IVY85_003054"
     CDS             complement(16113..16766)
                     /locus_tag="IVY85_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP8045981.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            16898..17740
                     /locus_tag="IVY85_003055"
     CDS             16898..17740
                     /locus_tag="IVY85_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8045982.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            17898..18794
                     /locus_tag="IVY85_003056"
     CDS             17898..18794
                     /locus_tag="IVY85_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8045983.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            18797..19510
                     /locus_tag="IVY85_003057"
     CDS             18797..19510
                     /locus_tag="IVY85_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP8045984.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            19530..20048
                     /gene="msrA"
                     /locus_tag="IVY85_003058"
     CDS             19530..20048
                     /gene="msrA"
                     /locus_tag="IVY85_003058"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8045985.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            20045..20272
                     /locus_tag="IVY85_003059"
     CDS             20045..20272
                     /locus_tag="IVY85_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP8045986.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            20424..20975
                     /gene="lepB"
                     /locus_tag="IVY85_003060"
     CDS             20424..20975
                     /gene="lepB"
                     /locus_tag="IVY85_003060"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8045987.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(21058..21600)
                     /locus_tag="IVY85_003061"
     CDS             complement(21058..21600)
                     /locus_tag="IVY85_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8045988.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(21837..>22020)
                     /locus_tag="IVY85_003062"
     CDS             complement(21837..>22020)
                     /locus_tag="IVY85_003062"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8045989.1"
                     /translation="GALISFSVENADEVKKFAQIAKENGGTFHEVDMGMPEEEMFSLE
                     VQDLDGNTLEPVWMKM"
BASE COUNT         7588 a         3728 c         4563 g         6141 t
ORIGIN      
        1 cttacggcag aatgttggtt aggaggacaa gtgttatgac agaatttgat caaaatcaag
       61 agaacaacca gtcaatggaa gacgcaatga aaagcgtaca agaagtaaaa gttggcgatg
      121 ttgttaaggg cgaagtttta gtcattgaag acaaacaagt gattgtcgga atcgaagggg
      181 ctggagttga aggagtcgtt ccggcaaaag aattatcaac tttgccggta gaagatatca
      241 gcgaagtagt aaaagtcggt gatgtattag atttagtcgt tatcagtacg atcggcaaag
      301 ataaagaaaa cggcagctat ctactttcaa aacgccgttt agatgctaaa aaagtttggg
      361 aagatatcga aagagatttc caagagggca aggtcatcga agcacctgtt acaaatgttg
      421 tcaaaggcgg tttagtcgtt gacgtcggtg tacgtggttt tgtccctgca tcaatggtgg
      481 aagatcattt tgttgcagac ttttcagact ataaaggcaa aacattgagc tttaaaatca
      541 tcgaaatcga accatcagaa aatcgtttga tcttatcgca taaagcggta gtggaaaacg
      601 aaagagaaga aaagaaaaaa gaaattcttt caactatcca tgagggagat gttctcagtg
      661 gacgtgttgc acgtcttact gattttggcg catttgtcga cctaggcgga attgacggtc
      721 tagtccatgt ctcagagatt tcacatagtc atgtagcaaa accaagtgat gtattggctg
      781 tcaatgatga tgtaaatgtc aaagtgttgt ctatcaatcc tgaacaagaa cgtgtttcct
      841 tgtcaatcaa agacacacag cctggacctt ggtcagaaat cgatacaaaa gctccagttg
      901 gcacagtatt agacggaaca gttaaacgtt tgacaagctt cggtgccttt gttgaagtgt
      961 tccctggagt agaagggcta gtccatatct ctcaaatttc acataaacat attgcgacac
     1021 cacatgaagt gcttcatgaa ggtgacgaag tgaaagtgaa agtattagag atccatccag
     1081 aagaacatcg tattgcattg agtatcaaag ctttagaaga aaaaccagct tcagaagaag
     1141 aaccaaaaaa tgttgagtct tatgaattac cagaagaaaa tactggattt actatgggag
     1201 acattttagg tgatgcgttg aaagaagaag tagaagaaaa agcaaccgat gattctgaaa
     1261 aataattgat tttgtatctg tgactgagat gaaagcagct gcttttgtct cagtttttct
     1321 tttgaaaagc gattagtatt tcaataagga ataaaaatac ttcgtttaat tgacaatcta
     1381 tgcttgaaag ttggcaatgc attcgttaaa ctaagtgaga ttggaaactt gtgaggagga
     1441 aataatatgg caaatccaac aattgcaatc gttggccgcc cgaacgtcgg taagtctacg
     1501 atttttaacc gcatcgctgg tgagcgtatc tcgattgtcg aagatactcc tggagtaact
     1561 cgtgatcgta tttatgcaaa aggtgaatgg ttaggtcgag aattcagcgt gatcgacaca
     1621 ggcggaattg atcttggcga tgaacctttt atggatcaga tcaagcatca agcagaaatc
     1681 gcgattgaag aagcagatgt aatcatttgt gtagtcagcg gacgcgaagg cgtgacagac
     1741 gcagatgaga tggttgcgaa aattttatac agaagtaata aaccagtgat tttagcagtc
     1801 aataaagtag ataatccaga aatgagaaac gatatctatg aattttattc cttagggcta
     1861 ggcgatcctt atcctgtttc aggaagccat ggactaggga ttggtgatat tttagatgaa
     1921 gcagtgaagt atttctctga agagactgaa gaagaggacg acgacacgat taagtttagt
     1981 ttgattggtc gcccaaatgt tggtaaatcc tctttgatca atgcgatttt aggagaagaa
     2041 cgtgtaattg tttcggatat tgaaggaacg acccgagacg caatcgacac ccacttcgtt
     2101 tctgaaagtg gtcagaaatt cttaatgata gatacagctg gtatgcgtaa acgcggaaaa
     2161 gtatacgaaa atactgaaaa atacagcgtg atgcgtgcaa tgcgtgccat tgaccgttct
     2221 gatatcgttt tgatggtact gaatgctgaa gaagggattc gggaacaaga caagcgagta
     2281 gcgggttacg cccatgaagc tggtcgtgga attattatcg tcgtgaataa atgggatctt
     2341 gtaaagaaag aaaccaatac aatgcgtgat ttcgaacaag agattcgtga tgaattccgg
     2401 tatcttgatt acgctccaat cgtttttgtt tctgcagtga ctaaacaacg gttggaacgt
     2461 ctgcctgaaa tgatcgaaca agtaagtatg aaccagaatc ttcgtatctc ttcggctgtg
     2521 ttgaatgata tcattatgga tgctgtagca atcaatccaa caccaacaga taaaggaaaa
     2581 cgcctgaaga ttttttatgc tacacaagtc gctgtcaaac cacctacatt tgtcgttttt
     2641 gtcaatgaag aggaattgat gcatttttct tatgaacgtt tcttagaaaa tcaaatcaga
     2701 aaagcattta cttttgaagg gacgtccatc cgaattatcc ctcgtaggag aaaataggca
     2761 ttttaccata tcgacgtttt tttttcaaaa gaaaaagaca gtttcgtttg ttacgtttct
     2821 atgactaaaa aaagcatgtg aaaattggta aaaaccttgc tattaagcgg ttcatatgat
     2881 atcgtgataa cagaatattg aagttaatca atatttcttt ttaaacttct ggaccactca
     2941 gaaagtttta actaaatgat atttctatca tatctcttgt ggaggaggtg aatctatcca
     3001 tggcaaacaa agcagaatta atcgaaaaag ttgcttcagc tactgattta actaaaaaag
     3061 atgcaactgc agcagtagat gctgtatttt caactattca agatgcttta gctaacggcg
     3121 aaaaagttca attaatcggt tttggtaact ttgaagttcg cgaacgcgca gctcgcaaag
     3181 gtcgtaaccc acaaactggt gaagaaatcg aaattccagc tagcaaagta cctgcattca
     3241 aaccaggtaa agcattaaaa gacgctgtta aataagacag ttgaaaaagc gcttgtggct
     3301 ctagggctac aagtgctttt ctttttattt cctagcagaa aaggaataaa taagagacag
     3361 agattccgaa tccgtatgtc atacatagaa aacacatgta ttttatttaa tttttattta
     3421 tttgcaagtt atgaggtgag ctttgctctt atcttttcta gaatttgctt actaatggta
     3481 aaataaaaat aagaagagga gggatgcgta tggaacttga ttatcaagta gaagatgcgt
     3541 tgacgaaagt gttttacgga gaaacgctag tcagtcagct aagagaactt tccattgatc
     3601 agccaatctt atttttaacg aatcagcgct attatgattt gtttgcagat aagatcaacc
     3661 cgttatttgc aaatcaagca aacaatgact ggtatatttg tgccaacact caatgcaatc
     3721 atttaacaga attaaagaac ctgcttgatt tcgtcaaacg ctatcccgaa aatcaatcca
     3781 tgtttatcat cggatttgga aatgaaggga tcatggatct ggcaggtttt tttcaaaaac
     3841 atacaaccct tgattcatca ctatggatga tccctgtatc gatccgttct ctaagccgag
     3901 cattaacacc gcagcggacg attttgcagc taccaaatcg tgtagtgctg caaaccatca
     3961 atctgccaga acgcatcgtt tatgatcaaa caatcagcga gcggcaggtg gacggaaagc
     4021 agattgactt tttgactttt atctgttgcg gaatgatttg tgatcatcgt tttttgcaaa
     4081 atctgtataa aaactatcca aataaaaagc aattaacgac tcggccgttt gccgggatga
     4141 tcgaagaaat gatctatttt tatcaattag aggcagaaaa cctgtcaaat tatggtaaac
     4201 tgtttgaaca agctttttat ctgaccgaaa atggccatct actttctgct agtatgaaaa
     4261 aatttttagg aatgatgttt catttcgtgt ggaatctcca agcatatcct tttgatttcc
     4321 aactaaaaaa cttttttgtg tggctggact tgcttggcta tccaatccac tggctggaac
     4381 agatttcaaa gatggagtat ttggaaaaag ttcttttttt ggcagaaaaa agcaaaaaaa
     4441 tacttgtttt agaaaaaata ggtataatag acggatacca acatccaaac gaaaaagaat
     4501 tattgagaac aatgacggta tttcaacaaa tcgtcagtga gataagaggg aattgaaatg
     4561 aatacaaata gcgaaaaaat gttgcaagct ttatcagatg aagacttgtc acaagcacaa
     4621 atctttttag aaaaagcatt aaaagaagac ccagcagata tattagctga actaggcgaa
     4681 gaattgctgg ctattggctt tttgatcgaa gcaaaacaga tttttgaaca actgatcaac
     4741 caatacccag aaaatgacgg actgaacatc ccgttagcag aaattgcaat cgaagataat
     4801 gagatcgact tagcttttga atacctggaa aaagtggata agcaaagtga ttactatccg
     4861 caaagcttat tggcgatagc tgatttgtac caagttatcg gaatccctga agtgagtgaa
     4921 gcaaaattga aagaagcagc tgggttgctt cctgatgaac cattgatcca gtttgcgcta
     4981 gctgaattgt atttttcagt tgatcgtttc aaagaggcag cagtggttta tgaaacattg
     5041 ttggcaagcg ggataaatga aatttcagga atctcgatgc aggaacgttt aggacaatcc
     5101 ttaagtatgc aaggagaatt cgaagcagct atcgtacctt tagaagaagc actgaaagaa
     5161 gaaaggacag atgatcgact gttccagcta gcttttacga atcttcaatt gaaagaaaat
     5221 cagcaggcaa tcaactatct ggaagaactt cgagaagtta atccgcatta tcaatcgtta
     5281 tatctatatc taggacaagc tcttcaagaa gaagaaatga ttgaagaagc acaaacagtg
     5341 ttagaagaag gaatcaaaga aaatccttac caagtcgaac tttatcacct ggcctcggaa
     5401 aatgctttcc gtctgcatga tagacagcga gcagaagaac ttttactgaa agctttagaa
     5461 gtaggagaca aacaagatga aacattactt actttaagta atctttactt ggatgatgaa
     5521 agatatgaag atgtgataca agtgatcaat cagatggaag aaactgctaa tccttatgca
     5581 gaatggaacc tagcccatgc ttataatgaa ttagaagact ttaccgttgc agctgtacac
     5641 tatgaacagg cttatcatga actatcacat gaaccagatt tcttgaaaga gtatgccttt
     5701 ttcttaagag aagaagggca gctgaaaaga gcgcaagaat tattgactta ttatttaaca
     5761 cttgaaccag gagatatgga ggcactgtca ttattagatg acctaacaga gaggtgagaa
     5821 aaatgctaat tgatgtacgg gaaaagaaga attttttaac gtggatggtc aatcatgtat
     5881 cttttagccg aagagaagtt ttttggatct tgaattatct tgccaatcat gaagcgattt
     5941 taagcaatgt ccactttgtt gaaggggcga attttgcgga tcgaggactg cagatcagtg
     6001 atctatctgt tgaaggggaa cccatgaaat tgtttttgca gggaaaagaa tttacggaca
     6061 cagaccagat ttttcatgaa atccgtttga attggaaaaa gcctttatat gtggaatgta
     6121 tattcgaaaa tgcttggcag acaagagaat atctatctat cttagaagac aatccatttt
     6181 cttcttggga ttcttcagtc agtgaagaag atactgaatt aattgaacga tattttcaag
     6241 aaaaagaaga agaggcaaaa ctaaaaatgc tttatgctca aatcgatcaa gcactagaag
     6301 atggagatag ggatgctttt ttgaaattat ctgatgaagt taaccgaaag atccttttgc
     6361 agacacaaaa gaaaagtaac cgggtaagtg aacataacaa ttagttaaaa atagtatagt
     6421 actaaaaaag acaaaggtca gctggatgtg gaatctttcc tcatagccta tctttgtctt
     6481 ttttcttata gttttttcag taatcttgtc tgtggcttca aataagtaaa gccttgccct
     6541 tccacttcat gcacattgat gattgaaagg aaagcttttt cgtcaatctc gtggacgatc
     6601 ctcttagctt cagttaattc actagagcta acgaccatat agatgatttt ttttgaatta
     6661 ccagagaatg cgccttctcc agaaaggaaa gtcacacctc tttgcagacc ggtcatcagt
     6721 agtggtgcaa tctcttcgct tttgttggag acgacaagta gaccttttgc agaatatccg
     6781 ccatctaaga tcgaatcaat cacacgactg aaaacatacg aaacaatcaa cgtatacatc
     6841 atacgtttca agtcgatata ggttaatgaa gcaagtaaga cgatgatatc aaatgccaat
     6901 agggaacgcc ccatagtgat gccgtaattt tgttctaaaa tccgagcgat aatatcactg
     6961 ccgcctgttg ttccacccat gcggtagacg atcccgcttc ctacacctgc aactaaaccc
     7021 gcaagtagag aaacgatcag tagatcatgc tgtaaattga tttctagtgg aaccctttgc
     7081 cagatccata aaaagaatga caaagaaagt gtacctagaa cagtataggc caaggaacgt
     7141 ttaccgagga tctttcctcc tagtaaaatc aatgggatgt tgattaataa agtcgtataa
     7201 gctggatcaa taccaaataa agctcttaag atcaaggtga tccctgagac tcccccttct
     7261 gctaaatgat tagcgatgtt caagtaaaca agaccaaatg cataaatact tgttcctata
     7321 atgataaaaa gaacttcttt tactgttttt cgtttgacca tatgaattcc agctttcaat
     7381 tatgtatttt cacagaattt ccatttagaa attccttcct aaatgtagca tggaaattcg
     7441 attttctcaa ttgctcttat ctaggatttg cgttaaagtt ttggtaagat agttttggag
     7501 ggaaaaagat ggataaaaag cgttctttat acagtatgca gcaggaagtg gatgaatata
     7561 tccagcaatt caaggtgggg tatttctctc cattagctca gatggctcga ttaactgaag
     7621 aagtaggtga attggcaaga gaagtcaacc acacttatgg agaaaaaagc aaaaaggctt
     7681 ctgaaccagt taactcggta gcagaagaac ttggtgatgt tttattcgtt acgatgatca
     7741 tggctaattc attgaatatc gacttaactg aagtttttga aaaaaacatg gaaaaattca
     7801 atcaaagaga ccataatcgc tttgagcgta aggacgagta aacaagatga agtggtcctg
     7861 gccttgaaga ctctgaaacg gacttgtcaa acagtcacca tgcaactgag tctttatctc
     7921 aggacaagaa acacgcagga aaggttggaa aaatgaaatt aaaacaatta cctctggaat
     7981 accaaaaggc aattccagtt ctgaaaaaat tagagaatgc aggatatgaa gcttattttg
     8041 taggtggaag tgttcgtgat atcttgctga accagccaat ccatgatgtg gatatcgcaa
     8101 caagcgcatt tccagaagaa atcaaacagc tttttccaaa aacgatagat attgggatcg
     8161 aacatggaac tgtgttggta ttagaaaatg atgaacaata tgagatcacc acatttcgga
     8221 cggaatcaac ttatcaggac taccgtagac cagatcatgt cgaattcgtt cgttcgttgg
     8281 cagaagattt gaaaagaaga gattttacga tcaatgcgtt tgctttgaaa gaagatggag
     8341 aaatcatcga tttgttcgat ggtctgcaag atctagaaga acagatcttg cgagctgtag
     8401 gcaatcctca tgagcggttc catgaagatg ccttacgaat gatgcgcggg ttgcgttttg
     8461 tcagtcaact agggttcaaa ttagaggaag aaaccttcta cgccatcaaa gaaaaccacg
     8521 agctattagg taaaatatct gtagaacgta taaatgtgga attcatcaag atgctcttgg
     8581 gaaatgaacg ggccgcggga atccatgcat ttattgaaac agagtgctac atttgctgtc
     8641 ccggtttgaa agaagaaggc gaagcacttt tacgttttgc tgaattacca tccgtcccat
     8701 tgaaagaaga gagccaagca tgggcattat tgatcagcca actggatcta aaagaaaatg
     8761 agattcgttc ctttttgaaa tcgtggaagt gttctaacca aatgattaaa gacgttcaag
     8821 cacttgtcca aggactacga caacgagaaa ctggagcttt ggaaccattc gaattgtact
     8881 tcttaggcaa aaaacatgca ctgcaagtag aagaattgat gatctatttt gacgaaacac
     8941 ctcagatgga tgccgtagga gaatcctatg atcgtttacc tatcaagtca ttaagcgaat
     9001 tagcagtaga tggtaaaatc ttgctagaag aaactggaaa aaaacctggt aagtgggtta
     9061 gtgaagcttt gcaattagct gaaaaagccg tcgtagaaca aagaataaaa aatgataaaa
     9121 aacaggtttt ggatttttta tcgaaagaaa aattgattta aaaaacacta gactgtatct
     9181 gctcagcttt gttggctact taaagtgggc agatacgtca ttagaagaaa aaacataaga
     9241 tgtgaccact tacataaatt tgtgctaaac tgaggtagta caagaataaa ttacatcata
     9301 aaggagtctt tttaatgagt aaagaaatga cagcgctaaa attttatttt agaaatggag
     9361 aaacctggac gatcaaccgc cgtcatatcg gcgatctttg gatcaaacaa atcacaacaa
     9421 gttttggacg aatcaatggc agtgaatttg ttgaaatcca tccgtgtgct ggatttaaaa
     9481 tcgaaatctt ccatgaaggg gacgcagttg cgacacatga tatcaaccta ggcggactgg
     9541 aaatgggcat gtttaatcgc gcattgaaat acgaagatat cgagcgaatg gaaatccttt
     9601 accgaaatgg cacacctgat cttgtgtact tcccatatct agataaagga acagaaggat
     9661 tagacaatca atatcaatca acaaaaatca gcgaaaaaac aggtaacttg tatatcgtga
     9721 tcaatccaga tcaacgtgta gaagatgtat acggggaatt tttcgaataa atcgattttt
     9781 acgcaaaaat gaggctgtga aagagatgtg tcttattact actgataggc tgtctcttcc
     9841 acaccgttta ttcagaataa gggactgtga caagattttt gtcgcagccc catttttttg
     9901 ttgtgataaa agaacaagaa aggcaggaat gggaaagtaa acatcctttt gtcagaaact
     9961 tttatctttc ttaagtttta cttgatatct gatactattt ggaatagaat gaggcgttga
    10021 aaaatttatg gaaatttcta actagcaata aacgatgaaa caatgaaata tttttcatcc
    10081 aaaaagaact ttgctataat gatggaagtt tgtgtatagg agatctggaa aatgagagag
    10141 agttttgaac aacaaaaaaa actgttgcat gatcgctatg gcgccctttc gatggatgac
    10201 cgccgtcaaa tactttgtaa gttgcgtaaa agaaatatat tgatgtatcg tcagttggaa
    10261 cgtctgaaac atgatctgct tcgtttggag tcaaaacgtg tacagtgtga attagagggg
    10321 aaccaaacac aggttgaagt agtcgaaacc aaaattttaa agaaaaaaga acaattttta
    10381 aaaatgctta cacaaaataa aaaataaagg ggatggacga tggaatttgc tgagttagtt
    10441 attgtatttg cagtaacgat caccttatcg aacatagcta gccgcatttt gccaatgatt
    10501 ccagctccac tgatccaaat tttccttgga gtggtattag gtctgacaga gtggggacaa
    10561 tcaatcgatt ttgaacctga attgttctta gttatgatta ttgcaccatt gttatttaga
    10621 gaaggagaaa aagcagatat ttcttcgatt ctcaaaaatt tcgggacaat tttgttcttg
    10681 gcatttggag gagtcatttt gacccttgtc ggggtaggtg cgacgttatc attcctgttg
    10741 ccaagtgtcc cacttgctgc ctgctttgct tttggagcag cgttaggtcc gacagatgcg
    10801 gtagcagtca gttctctttc tggacgagtg aatatcccta aaaaggcgat gcatattttg
    10861 gaaggagaag gtcttttgaa tgatgcatct ggtgttactg ccttccagtt tgctcttggt
    10921 gcgctgatca ccggatcttt ttcagcagta aacgcgggga tgagtctagt agtttctagt
    10981 attgggggag ctttgatcgg ctttttgctt gtctggttca aacaaaaaat aattcatttg
    11041 atcgaaaaag catcagcaca agatgtcaca gggtatctgc tgatcgaact tttgcttcct
    11101 tttctagcct atgtattggc agaggcattt gatgtatctg gaatcattgc tgcagtggca
    11161 gcaggtattc tccaagcttc tggttttagg aaaatctctg tgtttgatgc agaattgtcg
    11221 agcttgtccc acagtacatg gacaactatt gcttttacac tgaatgcatt agtatttata
    11281 tttttaggaa tcgaattgac gcaagtgttc tcgccagtat ggggagacgg tttatatcca
    11341 aacggtcttt tattagcgat tatcgtatta atctctgtca tgctttttgt gatcagattc
    11401 atttctatca gtctttttta tgtattcaaa gatggcagta aaaagttcaa aaaacagttg
    11461 aacgagatct tgatcttaac atttggcggt gtaaagggaa cagtcagttt ggctacgatt
    11521 tttattttgc cattttcaat caataatatg atgttttacc agcggtcttt gcttttgttt
    11581 ctgacggcgg gtgtcatctt agtgactttg gtcataggga tcattgtctt gcctatgctg
    11641 acggaaacag aggaagcgaa aagcacggat ttgaatgctt tgatgatttt agaagaggtc
    11701 gttgaagttt tgcgaaaaga aataaaggaa attgatcgta atacaaaaga atttttagcc
    11761 actgaagcag tgatagaaaa ttatcaagaa cggatacgtg atctgtactt ggaagattta
    11821 accgatgatg aaaagcaaga agtacaagaa atacaagcac tgattttgtc gatcgaacgg
    11881 gatggattgg atgaaagtta tcgttcgggc aagctttctt ctaatggtta tcgtttttac
    11941 tcccgttttc tttctcgatt cgaacattct atcacaagcc agattttgtc ttttatcggc
    12001 ttttggttca ttgttgtccg taggttaatg cgaatcgttc tccatccgaa aatgttttgg
    12061 caaagaagat atgctgatcg tcagacattt attagtgatg aagatatcca agaaatcaga
    12121 gagacatatc aaaaaaatac tcagttgatt attgaaagtt tagataactt gactgatatt
    12181 tatgatgaaa cgatggttca attttttatc cagcaacgca aacttgaagg aataaaaatg
    12241 aactcgggga atttgatttc ttcgtggatg atccagcaag atagtctgtt tacgaaaaaa
    12301 atgctaagag gttattatct tgaaagaaaa atcattgatg aatacgaagt tgctgaaaaa
    12361 atcacgacgt tttctgcgaa cagctatcgc aggaatatca atttgttaga atcttatacg
    12421 atgaacaaac catctgataa cttttctttc cgatttgcgt ttcgagcaaa gtcaaaaaaa
    12481 acgagtccaa taaaataaaa aggtaaaaat tttcaagttt tataaaaaac aaaacatatc
    12541 agaacaggat cgaatcggtc ctgttctttt tttgagaaca caaaaagaac aaataccaaa
    12601 aaaattttcc tgaagttcac tatttttatg atacaatatc caagataagg agggaacatg
    12661 ttgaaagaat taaaagtaac tgacctcaaa aaaacttatg gtgaaaaaga tttgtttgac
    12721 cagatttctt ttttggtcca cgaaaaagac cgtatcgggt tgattgggac gaacgggacg
    12781 ggtaaaacaa gtctcttaaa tattattgcc ggtatcgata gcagcgacgg tgatcggcaa
    12841 acagtgtttt atccaactga ttaccgtatt ggttatcttt ctcaagcagc tgaattttcc
    12901 gatgaattaa ctgttctcca agcagtcttt caaggaaaca gccccttgat ccaaactgtt
    12961 cgagcatatg aggaagcatt gatcgaacta ggggaaaacg gagaagaccc tgatgtgcaa
    13021 aaacgttatg ctaaagcaga agaacaaatg aataaagaag atgcttggac tacagatacc
    13081 aacgcaaaga ttattttgca aaaattagga atcagtgaat tagataaaaa aatcagtact
    13141 ttatcaggtg gtcagaaaaa acgagttagt ctggcgcaag tgttgattga tgaacccgat
    13201 ttactattac tagacgaacc aacaaaccat ttagattatg aagcgatcga gtggttggaa
    13261 aactatttga aacaataccg tggcgcacta ttgatggtta cccacgatcg ttattttcta
    13321 gatagagtag caaaccgaat cttcgaactt tcatttggaa aattatacga gtataaagga
    13381 aattatgaag cttacgtctt agaaaaagca gaacgtgatc gtgtagaagt agaacaagaa
    13441 gaaaagcgca agcggcttta taaacaggaa ttagcttgga tgagggctgg cgtacaggca
    13501 cgtggcacca agcagcaagc acggatcaac cgtttcgaag atctaaagga gaacttgtac
    13561 caagtaaacc aagaagatga tttagagctg aacctagcga cacaacgttt agggaaaaaa
    13621 gtactggaaa tcaaagatgg cagttaccgt ataaatgacc aaacactgct agaacattta
    13681 gatttattga ttcaatctcg ggaaagacta ggtattactg gtaaaaatgg tgcgggaaaa
    13741 tcgacattgc ttaatattct agctggcaga attcccctgg atagcggtac aatgtctatc
    13801 ggtgaaacgg ttcatcttgc gtattataca caagaaaatg aagaaatggc accagataaa
    13861 cggatgattg cttacttaca agaagctgct gaagaagcaa aaacagcaga tggcagtcaa
    13921 atcggagtag cagaattact tgaacgattt ttatttccaa gatttatgca tgggacactg
    13981 ataggcaaac tttcaggcgg agaaaaacgc cggttatttt tgttgaaact gctgattcag
    14041 caacctaatg ttttattatt ggatgaaccg accaatgatt tggacatagc aacattgacg
    14101 attttggaag attattttag aagttttcca ggtgctgtga tcactgtttc ccatgaccgt
    14161 tatttcttgg acaaggtagc agataagtta ttagtattcc aaggaaacgg aaaacaagaa
    14221 ctttattacg gaaacatgtc cagttattta ctgaagcaaa aagagaccca gcagcctgct
    14281 gaaaaagcga aaccaaaaat acagagcaag gaacctgctg aaaagaagaa attatcttac
    14341 atggaacaaa aagaatggga aacgattgaa gatgagattg cagaacttga agaaaaaatc
    14401 tcattgctcc aagaagagat gaatcatcaa ggagataatt tcactcgatt acaagaattg
    14461 caaaacgacg tatctgaaac agaagcacaa cttgaagaaa aaatggcacg gtgggaatac
    14521 ttgagtgaat gggtagaaga ttaggaggaa aaaggatgga acaagcgtat cttgacttag
    14581 gaagaaaatt gttggaagaa gggcatcgta aagaagacag aacaggcaca ggcacattaa
    14641 gcttattcgg ataccaaatg cggtttgatc tgcaaaaagg gtttcctttg ttaacaacga
    14701 aacgagtacc ttttggattg atcaaaagcg aattgctttg gtttttaaaa ggagatacga
    14761 atatccgcta tctattacaa aataaaaacc atatttggga tgaatgggca tttgaacgct
    14821 acatcaaaag tccagactat aatggaccag atatgactga ttttggccat agatgtttga
    14881 cagatcctga attcaatgaa gaatataaaa agcagagcaa gcttttttgc gagaagatcc
    14941 taaccgatga ttcctttgct gaaacatacg gcgatcttgg acacatctac ggctatcaat
    15001 ggcgtcactg ggaaacaaaa gacggtggct ttatcgatca aatcaaacaa gtgatcgaag
    15061 caatcaaaaa gactcctgat tctagaagat tgatcgtttc tgcatggaat ccggaagatg
    15121 ttcctagcat ggctttgcct ccttgtcata cgatgtttca attttatgtt caagagggac
    15181 ggctaagctg ccaattgtat caacgcagtg gggatgtgtt tctaggcgtt ccgttcaaca
    15241 ttgcaagtta tgcgttgctg actcacctaa ttgcacatga aacaggttta gcggttggcg
    15301 aatttgttca cacattagga gatgctcatc tttatatcaa tcatatcgaa caaatgaaag
    15361 aacaattgag ccgagaaata cgcgcatttc cgactttagt tttgaatcag gaaaagcagt
    15421 cagtatttga ttttgagatg gaagatatcc aattagaagg atatgaccca catccggcga
    15481 tcaaagctcc tatcgctgtt tagaatcaga aaggatgaga aaatgttagc tgcaatttgg
    15541 gcacaggatg aaaacggatt gattggaaaa gaagaccgtc ttccttggcg tttgccaaat
    15601 gatctgaaat tcttcaaaca aatgacagaa gcaaatacac tagtgatggg acgaaaaaca
    15661 ttcgaaggga tgggtagccg tccgttgccg aatcgtcaga cgattgtttt gacgagagat
    15721 tcttcctata aagcagaagg cgtgcatgtc atgcatagtg tggaagacgt gcttgctctg
    15781 gaaaaagaaa cagacggtat ctttttcatc gctggagggt cagctgttta tcaagaattt
    15841 ctgccatttt gtacgatatt ataccgtaca gtcatccatc atgtattcga tggagatgct
    15901 tattttccac ctgttgactg gtctgattgg tcactgatca atactagtca aggagaaatg
    15961 gatgaaaaaa atcgttatcc gcatcaattt gaaacgtatc agcgtaatga gaatcctgta
    16021 aaaagcggga caggcgaatt gacgatataa aaaagctatg gatagactag ctgttattta
    16081 gagctgtccc atccatagct ttattttttt tattaagtaa agaataaaac agagaagtac
    16141 ataaaacctg cacctaacat aacaaacaaa tgccaaacaa catgcatata gcggatagaa
    16201 ttcaagctat aaaagaatgc gcctatcgtg taagaaagcc cgcctgctac catcaaaata
    16261 acaccagtca ttccaagaga gtgataaaga ggaacagcag ccacgacaca gagccacccc
    16321 ataaaaatat agatgatcgt agatactttt gatactctct cccgtttatg caacgtcaac
    16381 gctttatata taatgccaag aatcgcaaac aaccagatta atacaaataa agtccaacct
    16441 agccatcctc ggatactcag caaacaaaaa ggagtataac ttcctgcaat caaaagaaaa
    16501 atcgaactat gatcgaatac ttggaacacc ttttttgctt ttgtaaaaat caatgaatga
    16561 aacaaagtag acgttaaaaa taaaagaatc atcatcgatc catagatagc ataagagaca
    16621 atatggaggg aggaatgcaa gtgggcacct tttactaaaa gaaccactag tcccgcgata
    16681 ctcaatccag tcccaattcc atgggtgact gcattaaaca cttcatttaa tatttcatac
    16741 gttcgattaa atttcgtttt ttccatgaaa acggctcctt tactaggtgt aattaatgaa
    16801 aaatcattat ttgtctgtta tactaaaaat agaaattgtc ttacatgtag tttaacatga
    16861 ataaggcatc ttgttaaaga aagagagaga gaaaataatg acaaatgtaa aaatcgtaac
    16921 agattcttca tgtacgatgg aaaaaaatgt acgcgatgct ttaggcattc gtgtcatgcc
    16981 gctatccatc atgatcgacg gaacagtata tcccgatgat gaccaactag acggcgaaag
    17041 atttatggaa atgatggcga aagcaaaaaa tctgcccaaa acaagccaac caccaattgg
    17101 agaattcatc tcgatgtatg atgagctagg agaagacggc agtgaagtga tttcgatcca
    17161 tatgacaaaa ggcttgagtg gaacagtaga agcagcaaga caagccagta atctaacaaa
    17221 aacgaaagta acggttatcg acagtgatac cactgaccaa ggattatctt ttcaggtaat
    17281 tcgagcagct gaattagcaa aagaaggtaa aacagtagag aaaatccttg aagaagtcaa
    17341 aaaagtacaa gacaatacaa aattatacat cggaatatcc acactagata acttggtaaa
    17401 aggtggaaga atcagccgaa caactggttt gctgtctagt gttttgaaca tacgcgtggt
    17461 gatgaatttc gcccattctg aattaatccc tgtaactaag gggcgaggaa agaaaacgtt
    17521 cgataaatgg tttaataatt tgaaaaatga actgaaagaa ttgccaaatg tgcgacaaat
    17581 cggtatttca catgcggatg caaaagaatt ggcgcaagaa ttcaaagagg gtttgcaaga
    17641 attattcccg gacatgcata tccctgtact gcatacgaat ccggttatcg ctacgcatac
    17701 tggtaaagac gcgtttgcaa tcatgtatta tgtagattag tcgaaaagag gataagccct
    17761 cagcctgaaa ggcttgcagc ttattctctt tttctttata aaattaggaa aataattgtt
    17821 ttcttttttc ataaagacta cctttttgat aaaatttttg ataacattag aaccaatcga
    17881 atacaagagg tgaaaaaatg aaattaacga aacgacattt gatcctcgtt ttgttcccat
    17941 tattaattgc gatcatcatc tatgcaggtt tagctgcttc tataccgaaa gctggcccac
    18001 taatgaaacc agtaactact gtgagagaag atccaaataa aaaagaaaaa cttcactaca
    18061 tagctatcgg agattcgctg actgaaggag taggagatca aaccaagcaa ggaggattcg
    18121 ttccccttgt cgcaaatgat ctaaaagacc gttataattt gaccgcggtt gaaacagaaa
    18181 actatggtgt aaatggtgaa cgaagtgacc agatcttaaa gcgagtaaaa acaaaagaag
    18241 aaattcgaaa caatatcaaa acagctgatt ttatcacact gactgttggc ggtaatgatt
    18301 tgatgaaagt tatccaaaat aatttattcg ggttaacggt caattccttt aaaaagccac
    18361 tgaaaaacta tcaggaaaat gtcacagaac tattggaaga atttcgatcc ctcaataaaa
    18421 ccgcacctat ttacatttta ggcatctaca atccatttta tttgaacttt cctgagatca
    18481 ctgacatgca gacaattgtt aacaattgga acaaaggaac agaaacggtg acaaaaaaaa
    18541 tgccgaactg ctactttatc ccaatcaatg atttgcttta tcaaggattg aatcaggaag
    18601 taggtgtcac agaagaagga acagatcaaa caactagcac gacagaaaat ctaaatggca
    18661 tcaaaaataa tgtgctctat gaagaagatc atttccatcc aaataattta ggttatcaat
    18721 tgatggcaaa tgcagtgaga gatcagctga tcgagacaca aaaccaatgg ttagagaagg
    18781 aggatgatca ataaacatga aaacaagatc tgacgtatct tcaaacaaaa caaagaaaac
    18841 aaattactgg aaattcgcct tccttatcct tcttggggta gtacttggtt cgaccgtatt
    18901 tttaggcagc cgtatttttg ctaatcggga accagaactc ccagaaatac cagctcttac
    18961 ggaaagacaa ggagatcctg tcttaacgat caattcaaat aaagaaaaag tcaatcaaat
    19021 catcagcttt ttccttagtg agtatcaaaa agatagtgat attgaatata aattttattt
    19081 ggaaaatgaa gcactactca atggtacttt cgaagtacta ggttttccta tcgatttcta
    19141 tttatatttt gatccgtatg tgatggaaaa cggaaatgtc caactaaaag ctagaagtct
    19201 ttctatcgga acattgagtt tgccaatcaa agatgtgatg aatatgatca agagaaacta
    19261 taaattacca gattggatcg aagtcaatac agatgatctg actatcatgc ttaggttgga
    19321 tcaattccga atgcaaaacg ggatgtatat taaagcagat aaaatcgact tagtaaatga
    19381 tgacattcgg tttagtctat atttgccaaa agatacctct gatgcttcaa acaactcaaa
    19441 taccaacaaa actagcacca gcagtactga aagcaagaca aaagaaaata aaaaatcatc
    19501 ggatgagtag tattagaggg ggaatcataa tggaacgagc aatatttgca ggaggctgct
    19561 tctggtgtat gatccagcct ttcgacacgc ttcctggtat ccatacagtg atgtcaggat
    19621 atactggagg gcatgtacca gatccgactt atgaacaagt aaaaagccaa acgactggac
    19681 atacagaagc agtggagatt atctatgatc caacattgat ttcatatgaa caattagtag
    19741 aactttactg gcagcaaact gatccaactg acgctttcgg acaattcgag gatcgcggtg
    19801 acaattatcg cccggtgatc ttttatttga cagaagaaca gcggatcatt gctgaaaaaa
    19861 gtaagaaaca gttagctgaa agtggacgtt tcgaacagcc aatcgttaca aaaatcgaac
    19921 cagcccaacc attttatcta gcagaagaag accatcaaga gtattataaa aagcatcctg
    19981 aaaattttaa aagaaaccat gcacgccggg ctgcctttat cgaagaaaat tggaggaacg
    20041 aagggtgaat cgaagctttt ttcattattt aatgacactg cgaggcggtg gcacacatga
    20101 tgctttatct gttttcgcta cagaagccgg taaagacatc caatttccta agcacagctc
    20161 tagctacgaa gaaatttcta attatctgga actgaatgtc gactatttgc cgtccatgga
    20221 tatctttgac gaagcatggg aaaaatattt agaaaataat catgacatat aagagatagt
    20281 tttgtacttt atgatcaaaa aagttttttg tccaatagcc taattgaaag aattattttt
    20341 atttcagcta tctagtcgca tttttttgat atttttctta taataaagaa agaatgaaaa
    20401 aaagagggat ggattgatag agattgacaa aaaaacaacg ctatattaat cgcttctggc
    20461 ttgtattcaa atacttactt gtttctgtat tccttgcgtt catgttgcgg ggatttttat
    20521 tcatcccggt accagtggaa ggcaattcaa tggaaaacgt gttgaagcaa ggtgatatgg
    20581 ttgtgatgga aaaattttcg gagatcagac gatttgatat tgttgttttt caattagcag
    20641 atggcacgat ttatatcaaa cgagtaatcg gtcttccagg agaaaatgtc agctaccaaa
    20701 atgatcaatt gaagatcaat gggaaagtag ttaaggaacc ttatctaact aaaaacctga
    20761 aatcagatca tgcaaatgca tcctatacga cggactttac actacaagag ctaacgggac
    20821 aatccaagct tccggaagat aattattttg tacttggaga taatcgaaga gtttcaaaag
    20881 atagtcgttc gtttggcacg atcaataaaa ctgatattct aggtaaagca agatttgtct
    20941 attatccact tgatgaaata aaatggatcc agtgagaaac aataaagcgt gcagtctgtt
    21001 gagactgcac gctttattgt ttaatccaaa atcagtttac taccttagta cgtaaaatca
    21061 aacagctcgt ttaaacactt ttctagcctc taaagttttg ataaatgaat aaacaaccat
    21121 tatttgaata gggatttgga tcatattcct gatgaatcta cctagaaaaa ttactttgaa
    21181 atctgcacca aacaaaacag acatcagcca agaattgata aaaaggctga ctgggatcat
    21241 aatcaataat tgggcagcga tgacggaagg caggtttatc tttttatgat ataaaaataa
    21301 gccattaata aatccaccaa ggaaagccgt gataagaaaa gcagcttgaa acgttcctga
    21361 accaaatatc gtgaaattta aagtgttcga caacatttca agcaaaccag cccagatcgg
    21421 tccatataga taacccataa gcgccatact taaaaatccg aatgtgattt gaaaataagc
    21481 aggaccaatc gctatcttgt ataagatgac atcaagggct aataaaagag cagaggtcga
    21541 caacaatctt gcattcattt cgggcaatct aaaaacgtct tttgcaatca aagtgttcaa
    21601 aatattctct cctttgtcag ttaataataa aaggatacca taaaatcacc aatgtgtcat
    21661 gacacattgg tgatttacat acactggcat ctaacaatta aaaaaagggt gagaagactg
    21721 cttttataaa ctgtaaaaaa gagattgtac gaataaaaaa aatagaggcg gagaatactt
    21781 atcgtatcat ccgcctttgt ttttttgtta taaaaaacgt cagattagtt atctgcttac
    21841 atcttcatcc atacgggttc taatgtattt ccgtctagat cttggacttc taatgaaaac
    21901 atttcttctt caggcattcc catatctact tcgtggaaag taccaccatt ttcttttgca
    21961 atttgagcaa atttttttac ttcatccgca ttttcaacac taaacgaaat caaagcacca
//
LOCUS       DACCYH010000259        16215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.guided.064, whole genome shotgun sequence.
ACCESSION   DACCYH010000259 DACCYH010000000
VERSION     DACCYH010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.guided.064"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(134..577)
                     /locus_tag="IVY85_003063"
     CDS             complement(134..577)
                     /locus_tag="IVY85_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP8045990.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="IVY85_003064"
     CDS             complement(795..2135)
                     /locus_tag="IVY85_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8045991.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="IVY85_003065"
     CDS             2404..3300
                     /locus_tag="IVY85_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP8045992.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="IVY85_003066"
     CDS             complement(3450..4391)
                     /locus_tag="IVY85_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8045993.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="IVY85_003067"
     CDS             4517..5638
                     /locus_tag="IVY85_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8045994.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /locus_tag="IVY85_003068"
     CDS             5896..7398
                     /locus_tag="IVY85_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP8045995.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="IVY85_003069"
     CDS             7465..7992
                     /locus_tag="IVY85_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8045996.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="IVY85_003070"
     CDS             8203..9264
                     /locus_tag="IVY85_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8045997.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="IVY85_003071"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="IVY85_003071"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP8045998.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="IVY85_003072"
     CDS             10177..12159
                     /locus_tag="IVY85_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8045999.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="IVY85_003073"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="IVY85_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP8046000.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="IVY85_003074"
     CDS             12881..14305
                     /locus_tag="IVY85_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8046001.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="IVY85_003075"
     CDS             complement(14486..15259)
                     /locus_tag="IVY85_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8046002.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="IVY85_003076"
     CDS             complement(15256..16161)
                     /locus_tag="IVY85_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8046003.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
BASE COUNT         5208 a         2949 c         3372 g         4686 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggaga
//
LOCUS       DACCYH010000260        15896 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.guided.066, whole genome shotgun sequence.
ACCESSION   DACCYH010000260 DACCYH010000000
VERSION     DACCYH010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.guided.066"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            <1..430
                     /locus_tag="IVY85_003077"
     CDS             <1..430
                     /locus_tag="IVY85_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HAP8046004.1"
                     /translation="ASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSP
                     STSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGV
                     SHVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            453..704
                     /locus_tag="IVY85_003078"
     CDS             453..704
                     /locus_tag="IVY85_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAP8046005.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            694..1923
                     /locus_tag="IVY85_003079"
     CDS             694..1923
                     /locus_tag="IVY85_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAP8046006.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            1920..4001
                     /gene="topB"
                     /locus_tag="IVY85_003080"
     CDS             1920..4001
                     /gene="topB"
                     /locus_tag="IVY85_003080"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8046007.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            4150..8070
                     /locus_tag="IVY85_003081"
     CDS             4150..8070
                     /locus_tag="IVY85_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAP8046008.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            8071..9015
                     /locus_tag="IVY85_003082"
     CDS             8071..9015
                     /locus_tag="IVY85_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8046009.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            9019..9222
                     /locus_tag="IVY85_003083"
     CDS             9019..9222
                     /locus_tag="IVY85_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8046010.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(9522..9758)
                     /locus_tag="IVY85_003084"
     CDS             complement(9522..9758)
                     /locus_tag="IVY85_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8046011.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(9941..10312)
                     /locus_tag="IVY85_003085"
     CDS             complement(9941..10312)
                     /locus_tag="IVY85_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8046012.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(10309..11637)
                     /locus_tag="IVY85_003086"
     CDS             complement(10309..11637)
                     /locus_tag="IVY85_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8046013.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(11598..11927)
                     /locus_tag="IVY85_003087"
     CDS             complement(11598..11927)
                     /locus_tag="IVY85_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP8046014.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(12185..12555)
                     /locus_tag="IVY85_003088"
                     /pseudo
     CDS             complement(12185..12555)
                     /locus_tag="IVY85_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            12700..12885
                     /locus_tag="IVY85_003089"
     CDS             12700..12885
                     /locus_tag="IVY85_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8046015.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            13828..14487
                     /locus_tag="IVY85_003090"
     CDS             13828..14487
                     /locus_tag="IVY85_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAP8046016.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            14490..15833
                     /gene="vanS"
                     /locus_tag="IVY85_003091"
     CDS             14490..15833
                     /gene="vanS"
                     /locus_tag="IVY85_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP8046017.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
BASE COUNT         3867 a         4199 c         4366 g         3464 t
ORIGIN      
        1 cgcctccacc ctgacgccgc cgacctacta cgacgtaccc ccggaggctt tggaggatgc
       61 cacctttgcc gccatgctca aagaggcgga aaaatatatc ggttatccct atgtgtgggg
      121 cggcagctct cccagcacca gctttgattg cagcggcttc atttcgtggg taatcaatca
      181 tagtggatgg aacgtgggac ggctgggggc gcagggccta tataacatct gcacccctgt
      241 ttccagttcg caggccaagc ccggcgacct tgtatttttc aagggaacct acgacacccc
      301 cggcgtgtcc cacgtcggcc tgtatgtggg caacaatgtg atgctgcatt gcgggaaccc
      361 gatttcctac acgaacctta cttcaaacta ctggcaatcc catttttaca gttacgggcg
      421 tttaccttaa cgccagaaag gagctttcaa ttatggcaac caaacttgac cgcatcgaaa
      481 aggacatcca gaaaaccaag tctaagatcg cggagtttca gaaacagctt cgggagctgg
      541 aaacgcagaa aaccgaacag gagaatttgc agatcatcca gcttgtgcgc ggcatgaaca
      601 tgaagccgga ggaatttgcc gcgttcttac gcagcggcgc aatgcaggcc gcccctgccg
      661 caacccctta ccatgaacag gaggacaatg cccatgaaga ataagaccag acggatttcc
      721 cgtattctcc ccgcacttct ggccgtgatg ctctgcgtca cggcctttcc cgttaccgcc
      781 cttgcaggcg gcaatgaccc ggcaccggcc ccgctgccgg aggctacaac cgaacccgct
      841 acgggcggtt tggagcctga aacagacgaa accggcaccg ccacggacgg gacagaaacc
      901 gaggccgaaa aagataccgg ccctctgacc ggcaaggaca tcaccgacct gttttccgcg
      961 ctgttcggtt ccaaggtgag cattgccgcc acggacgacg gcatccagat caccaccggc
     1021 accgacaaaa aagaacctga acagaccggc accgtcacca ccaacggcgg caggctgaat
     1081 gtccgcaccg gcgcggggct tgacaagacc gcgttcaccc agcttcccaa tgggacgacg
     1141 gttgaggtag tcggcacaga cggcgactgg attaaaattc tcttgccgga gcgcatcggc
     1201 tatgtccatt ccgactatat gaccgtcagc gaaaaggaag tagcggctac cggcgagggc
     1261 ggtttttccc tctccattga cccggaggaa attgcttctc tgctggaact gttcaatggc
     1321 ggcggggcca gcgccgccct gtccccggac gggaacctgt cactgattga cgacatcggc
     1381 agcgccgcga aaaatggcaa gcagtttatc accgtggaaa ccaaaaacgg caatgtgttt
     1441 tatctgatta ttgaccggga cgacgaggga aaagaaactg tccatttctt aaatcaggtg
     1501 gatgaagccg atctgctgac gctgatggag gacggggaaa ccgtcacccc cgctgctgtc
     1561 tgctcctgta ccacgaaatg caaggccggg gctgtgaaca cgaattgccc ggtatgtaaa
     1621 accaacctga ccgagtgcag cggcccggag ccgcaggaac cccagccgga ggaacccgaa
     1681 gccccggagg aagaacccaa gggcggcgcg ggcggcctga tcgtgttcct gctggtggcg
     1741 ctggcgggcg gcggtgcggc cctttactat ttcaagttca aaaagcccaa ggctgaaacc
     1801 accggcagcg acgatttggg cgagtatgat tttggggagg acgaggattt ggaggacgag
     1861 gaacccgaaa ccgagtttga cccggaagct gattttgtga gtgaacagga ggacgaggaa
     1921 tgacatataa acttgtaatc gcggaaaaac ccagcgtggc ccgcagcatt gcgggcgtga
     1981 tcggggccac ggagaaacac gacggctatt tggaggggaa cggctatctg gtgagctggt
     2041 gcatcggaca tctggtgtcc tttgccgacg ccggacgcta tgacgagcgt ttcaaaaaat
     2101 ggcgctatga ggatttgccc attctcccgg aaacatggca gtacatcgtc cccgacgaca
     2161 agaaacagca gtttgacgtt ctctgttcgc tgatgcagcg ccccgacgtg accggccttg
     2221 tgtgcgccac cgacgccggg cgcgaggggg aattgatttt ccgtttcgtc taccagatgg
     2281 cgggatgcca aaagcccttt gagcgccttt ggatttcctc tatggaggac agtgctatta
     2341 aggacggctt tgcccacctg aaaccgggcg gcgactatga tccgctgtat caatcggccc
     2401 tgtgccgggc caaggccgac tggctgatcg gcattaacgc cacccggctt ttttccgtcc
     2461 tgtaccacaa gaacctgact gtgggccgcg tccagacgcc gaccttaaaa atgctggtgg
     2521 acagggacgc caagattacc gatttcaaga aagagaaata tcacatcgtc catatcacgg
     2581 cgggcggcgc ggacgcggta agcagccgct tttccgacgc tgccgaggca aacaccgtca
     2641 aggcggcttg tgcaaaagcg caggccgttt gcgtttccgt gacgcgggag aaaaagacgg
     2701 aacagccgcc caagctctat gaccttacca ccttgcagcg ggaggctaac cggcttttcg
     2761 gctataccgc caagcagacc cttgactatg cccaggcact gtatctaaag aagctgctga
     2821 cctatccgag aacggatagt caatatctta ccgacgatat gctgcccgcc gtggaaagcc
     2881 ttgtgtccgg cctgtgggag ctggtgccgt ttgccaaggg cctgaacctt tccccgcagt
     2941 ttgaccgcat cctgaacagc aaaaaggtgt ccgaccacca cgccattatc cccaccgctg
     3001 aatttgtgaa gcagggcttt ggcactctgg ccgagagtga aaaaaagctg ctgtccctga
     3061 tctgctgcaa gctcttatgt gccgtggccg ggccgcatat ctatgaggcc gtcaccgcca
     3121 catttacctg tgccgggcag gagtttaccg ccaagggcaa gaccgtcctt tcccccggct
     3181 ggaaagagat tgaacgccgg ttccgttctt ccttaaagac cgacgccgac gaggacgccg
     3241 aggcggtgcg ggagctgccg gagctttgcg agggccagac ctttgcagac gtggccgcct
     3301 ctgtcaccga gcatttcacc acgccgccca agccctacac cgaagatacg cttttgtccg
     3361 ctatggaacg ggccggggcc gaggatatgc cggaggatgc cgagcgcaag ggattaggca
     3421 caccggccac ccgcgccgcc attctggaaa agctggtgca gatgggcttt gtccagagaa
     3481 aaggcaagca gctcattccc accaaggacg gcatcaatct ggctgtggtg ctgccggagg
     3541 cgcttacttc tccccagctt actgccgaat gggaaaaccg gctgaccgag atcgccaagg
     3601 ggcaggccga tcctgacgaa tttatggacg gcatcgagac gcaggcgcgg gagctggtgc
     3661 agacctattc ctgtatcagt gaggaaaagc agaaattgtt tcaggccgag cgtgttccta
     3721 tcgggacgtg tccccgctgt ggtgaggctg tctatgaggg caagaaaaac tactactgcg
     3781 gaaaccgggc ctgccagttc gtcatgtgga agaatgaccg attttttgag gaacgcaaga
     3841 aagcgttcac cccgaagatc gccgctgcgc tgctcaaaac cggcaaagtc aaggtaaagg
     3901 gcctctattc cgtaaagacc ggcaaaacct acgacgggac ggtgcttttg gctgataccg
     3961 ggggcaagta cgtcaactac cgcattgaac acaggagcta aaggacagta acacagcaag
     4021 catagggagt gtggctacac tcccggtaaa atcccccggc cgcgtccctg ccgggccggg
     4081 gatttttgct tgttggggaa gtccccaacc ccctattttt tgaaacagaa cagagaaagg
     4141 agtgaccgcg tgaacgaagt attttctatc caagtccata accggcagtt tttggaacag
     4201 ggcaaacaag gcgtctggct ggatttgccc accacaaccg aaacgctgca agccgccctg
     4261 cgggatgttg gcattactgc cgacaatccg caggattttt ttatcaacgg gtattcctgc
     4321 ccggaggaca gacaccttgc cattccctat gacatggtgc tggccgccga cgtggacgag
     4381 ctgaatttcc ttgccgcccg gctgggccag cttgacgccg ccgagatcgc agagctgaac
     4441 gccgccctgc aaaatccccg tggcggcttt gagaatatcg gccagatcat cgactacacc
     4501 gaaaatgtgg attactatgt gcatcttccc gacgttaggg gggatggaca gttgggcgac
     4561 tactacctga accgttccgg catggtggat atgccggagg aatggaaagc cgggattgat
     4621 acggcgcagt ttggacgcca cattgcccag caggaacaag gcgtttttac ccagtacggc
     4681 tatctggtga gaagcggcga cgaatggcag cgcgtccatg agggccagcc ggtgccggag
     4741 gaataccggg tgatgcacta cccccagccg gaagctgacc gggaggaacc gcaggcccag
     4801 cagccggagg catccgaaag ccccgacaaa gcgccgcccc cggttatccc gatcatgctg
     4861 gacggcatga acagcaaaga acgcatgaaa gtgattactg accgtctgga aaccggcgta
     4921 caggagcttt ttgaaagtag ccgctatatg gcttacttat ccgctatgtc caagatgcac
     4981 gattacagtc tgaacaatac gctgctgatc gccatgcagg gcggttcgat tgtggcgggc
     5041 tataacaggt ggcaggatga atttgaccgc catgtgaagc ggggcgagaa aggcattaag
     5101 atactggccc cggccccgtt caaggttaag cagcagatgg agaaaaccga cccggccacc
     5161 cagcagcccg tgatcggcgc ggacggcaag cccgtgaccg aagaacagga agtgacggtt
     5221 ccggccttta aggtggtgac agtctttgac atcagccaga ccgagggcaa ggattttccc
     5281 atgccctata tcagtgaact taccggcgac gtgcagcagt atcaggattt ctttgccgcg
     5341 ctggaacgga catcacccta tcccataagc attgagccga tttcagacgg ggccaagggc
     5401 cgctgcttct acgcaaaaca gcgcattgcc atcaacgagg gcatgagcga attgcagacc
     5461 ctaaagaccg ccatccatga gattgcccat gccaagctcc acgccattga ccgggaggcc
     5521 cccgccgagg aacaggcggc ccggccagac cgcagcaccc gcgaggtaca ggccgaaagc
     5581 gtggcctata ccgtctgcca gtattacggg ctggacacgt cggactattc ttttggctat
     5641 gtggccgatt ggagcagcgg cagggagtta tccgagctga aagcctccct tgaaaccatc
     5701 cgggccacgg ccagcgaatt gataaccgag atcgacggcc attttgccca gcttcagcag
     5761 gagcgcgagg cccggcagga acaggaggcc gtcacggttg acgttacccc ggaggcagcg
     5821 gccccggcgc aggccgagga agaacaggcc ccggcccttg atccggcagc cgagccgctt
     5881 gtgactgtgc tatggagcga aagcccccag ctaaaggacg gccagcaaat gccgttgcat
     5941 gaggccgacg cgctgtttgc ccgtctggat gcagcatatc cggcagaggc aggctatgac
     6001 aaaaccgcgt tccgcattga ctgtatcttt cagggcgagc cggacagcta cgaggggcgg
     6061 caggattttg gagataggga cggttccctg atcgaacata tccagagcta ccaccaattt
     6121 tacgcgcagg acgaaaactg gaaaaagcat gtaatcgcta caaccggcat agagggctgg
     6181 gagcaggaca aggcccatcg ggatatgctg ttaggcgagt ttgtccccta tatgaagctg
     6241 cattgcagcc ttgcccaaat ggaacagacg gcgcagggcc gcttacagtc cggggaaacc
     6301 ctgacgccgg aggaaacggc ctatttgggg gcgcttctct cttatgtgca ggagtgccgc
     6361 cccatgctca atcagggcca gtatcagttg ccggagccgc cgaaactggc tgacttcgac
     6421 cagagcttgc aggactacaa agcacaggta caggccgaga tcgcgcagga ggccgccgca
     6481 gccggtatga ccgtggagga atacgcggcg gctggctatg aagcacccca acaggacacc
     6541 ttttccatct accagcttcg aggcggggag gaaacccgcg actatcggtt tgagccatac
     6601 gagcgtttac aggccgcagg gctggccgtg gacagtgcca actatgacct tgtttatacg
     6661 gcaccgctgg ataaaagcac gtctttggag gatatttacc gcacattcaa catcgaccac
     6721 ccggcagatt ttaggggaca cagcctttcc gtttccgacg tggtggtgct gcaccagaat
     6781 ggacaggaca ccgcccacta tgtagacagc ttcggctata tggaagtgcc ggagtttttg
     6841 cgggaaaatc ccctgcgtac cgccgaactg tctacggagc agaacgaaaa catgattgac
     6901 ggcattatca acaatacgcc gcccgaaccg cagccggagg aacccgaaca gctatctttt
     6961 gaggatgtgg cagttgcttc cggccagaca caggaggaac ccccggaggc tgcgcccccg
     7021 caaacggctg tccgctatta tcccatcaat gagggagccg cccgccgcgc caaggaagcc
     7081 atgagctttt ctgactaccg gcccggcagc gccacggcgg aataccggca ctatgtcgat
     7141 caggccgtgg aaattgcgga gcgccagaaa aagcgtgtgg agccagagta tcacgaaaag
     7201 atagacagcc tccttgacac ctacgcccgc aagctggccg agaacatgaa taagggctat
     7261 gagattaccg cccgcgtccc ctctgtcctg attgccgggc cgtccaattt cccggtacgg
     7321 gcgaaagaaa agcagaacgc cgccagtgac cgcaacatgg aggaatttca gtacattcag
     7381 ggcttgcttg acaaaatccg cagcaccggc atgggtggta tcagcgccga cgatccgcag
     7441 gccgtttcca agctggaaaa gaaactggaa aagctggaag cgtcgcagga gttgatgaag
     7501 gccgtcaatg cctactaccg caagcatggg acgctggacg gatgccccca tctgatcgaa
     7561 agaggcattg aaaacctgaa agcggatatg gcgtcgggct ggcactatga gaaaaaaccg
     7621 ttccagtcct ggcagctctc caacaataac gccgagatac gccggttaaa gggccgcatt
     7681 gaggaattga cccgccaaaa ggaggcggcc tatgtgggat gggagtttga cggcggcact
     7741 gtggagatca accgggtggc caaccgcctg caaatcttct ttgaggggaa gccggatgct
     7801 gctgtacgcg acgagctgaa aagcaacggt ttccgctggt cgcccaaggc cggggcatgg
     7861 cagcggcagt taaacgacac cacaatccgc gtcgctgacc gtatcaagtg catccagccc
     7921 ctttccggcg aaaagcccag cgccttacag cttgcggccc gcagggagaa agaaaagccc
     7981 tctatccggg cgcagctcaa cgcggccaag accgaacagg ccaagcagcc accggcgcgg
     8041 gaaaaatcaa agggattgga ggtagagtaa atgcagaagt ttgaaaatgt ggacgttctg
     8101 gccgcgctgg aacagatcat gcggcagaac accgcgtttt atcaaagtga ctttggcatt
     8161 gacaaagaca ttatcaggcg ggatgccgcc agcgatcagg ccgcagacaa gaccctgctc
     8221 tggatgtccc ggccctccgg gacgtactgt ttccgggaac gtgatgtttt cctgaaagac
     8281 acccgacagt acaacacatg gaaattttac ggggagcaga cccgtgacaa gatactggcc
     8341 tacgcggtgg agctgaccgg cacacagggc ggcactatcc ggggcaatct ctatgagctg
     8401 gactatccgc agcatttccg gcacgtcatt gaaaccgccc agccggtgag tgtgaaccgg
     8461 ctcttttatg agcatggcac ccgtgacatc ccggagagcc agcgttttga cggaagccct
     8521 gaccgggtat tgggaaactt tttgtactat gaggcccagc cccatgaccc ggctgtgctg
     8581 caggaggcgc tacggacgga gcagcacagc cgggggcggg ccgtccccgg cgatttcaaa
     8641 gtccacgtca ccgctttgca tgacagccgg attgaaaccg aggcccggcg tattgtggac
     8701 agcctgaaag atctgtccgc gcctaacagc cccaataaaa cgcatttcat ggtggagatc
     8761 tccccgcact ttgcggcgat tgcttccacc aaggataatg accggctctt tgccatgctg
     8821 ccctataaat ccctgtgttt taccgggatg aaaggcaggc atggcgtgta tgccgtgatt
     8881 aaccgggacg agcgccggga tgtgagtatt cgcaggcccc ggccctctat ccgcaagcag
     8941 ctatccgaca gcaaacaggc cgccagccca aaaaaggcgg cggccagaac caaaaagaat
     9001 gaattggagg tataaggaat gatgcacttt accgtggagg aagaaaacct gatctgtatg
     9061 taccacaatg cagaccggcg cagaacgata aacaggctca ccgccgtcat gccggttatg
     9121 gatggggata tgcgggcgca ggcccagcag accctttcca agctggaacg catgacggat
     9181 gccgactatg acggccagaa gttccacttc acagacgaat aacgaacagc agacgccggg
     9241 cgcggcggcc atgtggtcgc ttgcgtccgg cgtcttttct ttttgcctat tcctacgcat
     9301 ttagaacccg gtattgtcgg ggataggatg aagtattcgc ttgcccctgc aaacatgcgg
     9361 gagaagcctt gcagcgggcg gttttcaatc ccttaaatgc gtaggttgca gctataatta
     9421 gacccctgaa acagatatag ccgcgcagcg ggtgtatctg tttcaggggt gtgcaggtga
     9481 tagccgcaaa gcggcgtaag agggtgcagc cctcttgccg tctatctctg ttccagcggg
     9541 aagctcggca atgcttccag cagtttcagg gaaatgcgct gccgtatatc ttcgtcaatg
     9601 actttcttct ttttgccgtt tgcctgtctg atctccaccg tggaaagctc gttgatgtag
     9661 tcagcataat gggccaacac tttttccgtg gcccacctct caccggcaac ggcggctttg
     9721 atcgtttcat agtccagcag cttttcttgt ccgtccatcc tgataccccc gtcagactga
     9781 ttttttctat gataccatat tcggaagcat ggaaccagag aaaacggcgc ttttctgctg
     9841 gtaaattcta cgcatttaga acagcacttc ggcggggata ggataaaaca cccacatacc
     9901 ccgctgacca tgccggaaaa gccttgaaat ataagggctt ttacagctct aagtgcgtag
     9961 gtttccagta tcttttccgc tggcgctcgg cctctttgcg gcggcgcatc cgtttggagc
    10021 aggccgcgca gtatttggcc cggttagact tcggcgtgaa cgtggccccg cactcacagc
    10081 agcgtttcac ttcgtcccgg tctttggtga tctcggcata cagggccgca tccagcggga
    10141 ggacagcggc cttaaaccag cggcacagaa gcgaatagga aatgctctgt acgcaaatac
    10201 attcttcgcc gtcgtccagc aacaggcagt tgccgtcaca atagttgcag cactcatgcg
    10261 tcagcttccg ggccttgcga tattgagggt aattcattcg tggaatactc atagctcttg
    10321 cgtccattcc tttcccggcg tcatacgcaa aatgccgtcc acattctgct tgatgatgcc
    10381 gtattcctgc atctgctttt tcgcttcgcc gtacttctca tacagccgtt ccttttcctc
    10441 ggccaatcgc ttgtactcgg ctttcagggc gtacagatcg gggagctttt tcacaccctg
    10501 tccctttagg gcctttgccg cagcttcata caaaatcaac gtgctttcat gctcacgccg
    10561 gaattttgcc ttatccgctg cattgcggta ttccatagcc acgggccaga gctgcttgta
    10621 ggtagaaata ttctttatca gcaccgccat atccgcaagc cggtgttcca tagctttgag
    10681 ggtagcggcg gcctcgtcgc tggcggcggt gatctcggcc actttgcttt ccaaatccgg
    10741 gtaggtgtct atcccatgtt cagtcaggaa attcaaagtc tttgcggcct gcttcaaatt
    10801 gtggagcttc gcccacctct catagcctgc cgactgctgg gccttaatgt tgttttccag
    10861 atcaatccgc aaagaaatcc ccttgcgctc tttgggcgct ctggtgataa cgcggccttt
    10921 gatacgctct ttgatggctt cctcggtgta ggcttccccc aatgtcttgc agcgggtaaa
    10981 gcgttcctgt ccgggagcgc ggaaagaaat aaactttccc ggcttgatct catagccctg
    11041 cgcctgcatg agccgcagga aatcatcaaa atcttttgcc tgcgggatag cggcgtcaat
    11101 ggctgctttg agcttcgctt tccagctcgt ccctttccgc tgggcgtccc actcggcata
    11161 gctcttgccc ttactctggc cgggcttcac cacggacaat ccgttttcct tgcacagcct
    11221 gtcgctggtg cgccggatat aggcatagct ctgtttgttg gaaatgtact tgcgctgatt
    11281 taccatatcc acggcacaga aaataatgtg attgtggacg tggcccttat caatatgggt
    11341 agtcagcaca tagggatatt tcccttgcag cacttcctcg gcaagctgct tcccgatctc
    11401 atgggcctgt tccggcgtgg cttcgccggg ggcaaaggat tgtatcaggt ggtggcccag
    11461 attattgccc ttttggtatg cctgcgccag cagcatttca aattcaatgt ccgctgtttc
    11521 ataagagcag gcaaaggagg ccaccagcag cttgccgtca gttttgtccg ggttctcgat
    11581 gtagtcaagg gcttttttca gcgttgactt gataggatgt atctttgtaa ctgccatatc
    11641 tgcgccagcg ccccctttat atcctcgatg tcctgtgcgt acaccggccc cataccgttc
    11701 agcctctttg caatctgatt gacgttgacg ccgatcttct gtatctcggc ggtctgcgcc
    11761 cggatgtcgc tggtgtccat gttgacgata tagccgtcaa tgagcatctt ccgggcgtag
    11821 gctgaaaagt tcttcgtatg cagcagcgcc attttctgtt caatcatagc ccgttcctcc
    11881 ggcgtcacag ggacgcgcag cacgattttt cgctttctgt ttgccatgct tcaccgtcct
    11941 ttccattcag gggtttgggg atttccccaa caagcaaaac ggacgcggcc cggcagggac
    12001 ggggcaggcc gtttttccgg gagtggctac actccctatg cttgctacgt tactgtttct
    12061 ccactaacaa gacgtttcca aagccccaaa ctacccgcaa aatagccctc tggaaagaaa
    12121 aaagtgcaaa aaaatagaag ctacgctgtg cagctcccgc taaaagtaaa acagaggatg
    12181 ccagttacac atcctccgtt tcattcttct tcggcttttc aattagtatg gcgctgtcaa
    12241 tcgcgccctc gatgatcggc aggtattctt cggggcagag tttcagcttg tggctgaccc
    12301 tttgccgcag ttcgctttcc tcccgcatga tctccgggtt aaagtagcgt tccacaggaa
    12361 gcccgcaggc tttaataagc tgtatgacaa ccggcagact gggaatggcc cgtcgttttc
    12421 gatgttggca agatagcgcc agtcaatatt tattttctct gccattgctt tgcgcgaaac
    12481 gcgctttgct tgccgggccg ctttcacatc cgcgccgaaa gtttcaaagc cgggacaatc
    12541 ttcaatattc gccatatcgc acctcccact tacattgtat atttcatact ctgcccgtgg
    12601 aatgttggta tatgcacctt tcttaaagtt tatatttcac attttgtttc ttttattctt
    12661 cgagcaaaag aattataatg taccctgtgg aggtgtacta tggactacat gacgttgaaa
    12721 gaggcaggcg agaaatgggg tgtgccccca cgatggataa actactattg tgctgcggga
    12781 cgcatccccg gcgctgtaaa gatggcgacg atctggctca ttcccaaaac tgcggaaaag
    12841 ccgattgaca ggcgtacaaa gaaaggaaag gcggttactg aatagtataa tgaaaccggc
    12901 gcattttgga tatggttttg aagagagaga aagcatggta ttttgtcacg gaaaaactat
    12961 aagatgatag atggcaggct gctccagacc gataaaaagt attctcatct gaaacagaaa
    13021 gaaaagattt acacatggat gtatgaagaa gcgaaacttt atcatgacag aaatggaaaa
    13081 tgcccgaaac aaagacgagg aagctgctgc tgtatcagcc gtatatggta gaattgaaaa
    13141 ggccggcatc tggattcttt atggtgaagt ctccagacat tggataactg ctggtctaat
    13201 gcgcctttga gtatggggtg attgatttta ataccgggga ggtacggctt tttgaggaaa
    13261 atattctggg atttacattt ggcgattatt acatttcctg taactgcaag gacacggatg
    13321 atgaagatat gaggaatata gacttgctga ttttctactg tcttgaacgg tattaggact
    13381 gaatctgctg cgcggagtgc ggcaagagcg accggaggga gtacacacag gccctgcctg
    13441 tgtgaaaagc cataccgcaa ggtgcattcg caaaaaagag gccgcgcatt ttgctgaggc
    13501 ttggggtgtc cccaacaagc aaaacgagaa cgccgcaggc gggcaagtgg tggatcagaa
    13561 tatccgtaaa ggatattctg atccattgct tgccagtatt atcgggatgt tttgacaacc
    13621 gctgtaatcg aaacatccca aacattaaga aggtgttaat tattattatc ttgatttaat
    13681 tcgtttaaac caataatata gatacataca ccatgaaaag aagcgagtat cccaatgacg
    13741 gatgattaaa atccgaaaag aaatcgcttt gaaactacag ggaaactaca gactgttatg
    13801 ttatcttctt aaaatggagg gatttttatg tcgatacgaa ttctacttgt cgaggatgat
    13861 gatcatatct gtaatacagt aagggcgttt ctggctgagg caggatatca ggtggatgcc
    13921 tgcacagatg gaaatgaggc atacaccaag ttttacgaaa acacttatca actggttatt
    13981 cttgatatta tgctgcccgg tatgaacggg catgaacttt tgcgtgaatt tcgtgcgaaa
    14041 aatgatactc ccattctgat gatgacagcc ctgtcggatg acgaaaacca aatccgggcg
    14101 tttgatgcag aggcagacga ctatgtaaca aagccattca agatgcagat tttactaaag
    14161 cgggtggaag ccctgctgcg gcgcagcggt gcgctggcaa aggaaatccg tgtcggcagg
    14221 ctgacacttc tgccggagga tttcacggta ctttgtgacg gtacagagct gcccctgaca
    14281 cgaaaagaat ttgaaatcct tttgctgctg gtgcagaaca aaggcagaac cttaacgcat
    14341 gaaatcattt tgtcacgtat atggggatat gactttgagg gtgatggcag cacagtccac
    14401 actcatatca aaaatttgcg ggccaagctg ccggaaaata tcatcaaaac cattcgcggt
    14461 gtaggatacc gattggagga atcataataa tggaaagaaa agggattttc attaaggttt
    14521 tttcctatac gatcattgtc ctgctactgc ttgtcggtgt aacagcaaca ctgtttgcac
    14581 agcaatttgt gtcttatttc agagtgatgg aattacagca aacagtaaaa tcctatcagc
    14641 cattggtgga actgattcag aatagcgata ggcttgatat tcaagaggtg gcagggctgt
    14701 ttcactacaa taaccaatcc tttgagtttt atattgaaga taaagaggga agcgtactct
    14761 atgccacacc aaatgccaat acatcaaata gttttagacc caactttctt tatgtggtac
    14821 atagagatga taatatttcg attgttgctc aaagcaaggc aggtgtggga ttgctttatc
    14881 aagggctgac aattcgggga attgttatga ttgcgataat ggttgtattc agccttttat
    14941 gcgcgtatat ctttgcgcgg caaatgacaa cgccgatcaa agccttagcg gacagtgcga
    15001 ataaaatggc aaacctgaaa gatgtaccgc cgccgctgga gcgaaaggat gagcttggcg
    15061 cactggctca tgatatgcat tccatgtatg tcaggctgaa agaaaccatc gcaaggctgg
    15121 aggatgaaat cgcaagggaa catgagttgg aggaaacaca gcgatatttc tttgcggcag
    15181 cttctcatga gctaaaaacg cccatcgcgg ctacaagcgt tctgttggag ggaatgcttg
    15241 aaaatatcgg tgactacaaa gatcattcta agtatctgcg cgaatgcatc aaaatgatgg
    15301 ataggcaggg caaaatcatt tccgaaatac tggagcttgt cagcctgaat gatgggagaa
    15361 tcgtacccat agctgaaccg ttggacatag ggcgcacggt tgccgagttg ctgcccgatt
    15421 ttcaaacctt ggcagaggca aacaaccagc ggttcgtcac agatattcca gccgggcaaa
    15481 ttgtcctgtc cgatccgagg ctgctccaaa aggcactatc caatgtcata ttgaatgcag
    15541 ttcagaacac gccgcaggga ggcgaggtac ggatatggag tgagcctggt gctgaaaaat
    15601 gccgcctttt tgttttgaac atgggcgttc acattgatga tactgcgctt ccaaggctgt
    15661 tcaccccatt ctatcgcatt gatcaggcgc gaagcagaaa aagtgggcga agcggtttag
    15721 gacttgccat cgtacaaaaa acgctggatg ccatgagcct ccagtatgcg ctggaaaaca
    15781 cctcggatgg cgttttgttc tggctggatt taccgctcac atcaacattg taaatattta
    15841 aaacttaaat gattttgggc tctttgtcaa ataggactga tagagtgaga taagaa
//
LOCUS       DACCYH010000261        13213 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.guided.082, whole genome shotgun sequence.
ACCESSION   DACCYH010000261 DACCYH010000000
VERSION     DACCYH010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.guided.082"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            complement(204..2162)
                     /locus_tag="IVY85_003092"
     CDS             complement(204..2162)
                     /locus_tag="IVY85_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8046018.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="IVY85_003093"
     CDS             complement(2159..3031)
                     /locus_tag="IVY85_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8046019.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="IVY85_003094"
     CDS             complement(3373..4245)
                     /locus_tag="IVY85_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8046020.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="IVY85_003095"
     CDS             4373..4942
                     /locus_tag="IVY85_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP8046021.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="IVY85_003096"
     CDS             5009..5746
                     /locus_tag="IVY85_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8046022.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="IVY85_003097"
     CDS             5977..7245
                     /locus_tag="IVY85_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP8046023.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="IVY85_003098"
     CDS             7732..8196
                     /locus_tag="IVY85_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8046024.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="IVY85_003099"
     CDS             8180..8809
                     /locus_tag="IVY85_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8046025.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr"
                     /locus_tag="IVY85_003100"
     CDS             9163..10641
                     /gene="msr"
                     /locus_tag="IVY85_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8046026.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="IVY85_003101"
     CDS             10688..11134
                     /locus_tag="IVY85_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8046027.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="IVY85_003102"
     CDS             complement(11333..11848)
                     /locus_tag="IVY85_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8046028.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="IVY85_003103"
     CDS             11965..13167
                     /locus_tag="IVY85_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8046029.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DACCYH010000262        10087 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.guided.103, whole genome shotgun sequence.
ACCESSION   DACCYH010000262 DACCYH010000000
VERSION     DACCYH010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.guided.103"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            537..1139
                     /locus_tag="IVY85_003104"
     CDS             537..1139
                     /locus_tag="IVY85_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8046030.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1155..1832
                     /locus_tag="IVY85_003105"
                     /pseudo
     CDS             1155..1832
                     /locus_tag="IVY85_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2344..2640
                     /locus_tag="IVY85_003106"
     CDS             2344..2640
                     /locus_tag="IVY85_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HAP8046031.1"
                     /translation="MSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTNSASGVLRAN
                     EIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLKEKVVR"
     gene            2641..3057
                     /locus_tag="IVY85_003107"
     CDS             2641..3057
                     /locus_tag="IVY85_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP8046032.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3077..4621
                     /locus_tag="IVY85_003108"
     CDS             3077..4621
                     /locus_tag="IVY85_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8046033.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            4764..4988
                     /locus_tag="IVY85_003109"
     CDS             4764..4988
                     /locus_tag="IVY85_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8046034.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            5003..5872
                     /locus_tag="IVY85_003110"
     CDS             5003..5872
                     /locus_tag="IVY85_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP8046035.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            5853..6587
                     /locus_tag="IVY85_003111"
     CDS             5853..6587
                     /locus_tag="IVY85_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8046036.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            6620..7528
                     /locus_tag="IVY85_003112"
     CDS             6620..7528
                     /locus_tag="IVY85_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP8046037.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7525..8067
                     /gene="sat4"
                     /locus_tag="IVY85_003113"
     CDS             7525..8067
                     /gene="sat4"
                     /locus_tag="IVY85_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAP8046038.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8160..8954
                     /locus_tag="IVY85_003114"
                     /note="aph(3')-IIIa"
     CDS             8160..8954
                     /locus_tag="IVY85_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP8046039.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(9062..9250)
                     /locus_tag="IVY85_003115"
                     /pseudo
     CDS             complement(9062..9250)
                     /locus_tag="IVY85_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9230..9802
                     /locus_tag="IVY85_003116"
     CDS             9230..9802
                     /locus_tag="IVY85_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8046040.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
BASE COUNT         3548 a         1572 c         2207 g         2760 t
ORIGIN      
        1 acaagaagca ttttattcta gcttaatttg acaaatcata ctatgatacg tgtttgtttt
       61 tctgtgctgt ttagtgaatg attagcagaa atatagagag taagatttta attaattatt
      121 agggggagaa ggagagagta gcccgaaaac ttttagttgg cttggactga acgaagtgag
      181 ggaaaggcta ctaaaacgtc gaggggcagt gagagcgaag cgaacacttg atcttttaag
      241 ttgctatctt tttaaggtca atagagtata cttatttgtc ctaatatggt tttagcagta
      301 taattgactt ggtgaatagg tcatttaagt tgggcataat aggaggagta aaatgaaaaa
      361 atttatttat cgagttttag aaaatgacga agtggtggct atttttaatg agcaacaata
      421 tgcgcaagat tttatcgctt acgaaaagac aatttctgat aagcaatttg aaattgaaaa
      481 agtagatata gctgattggt tattgcaacc gagagaattt tagaggttgg ttgaaaatgg
      541 ctaaaattgg ttatgcacgt gtcagtagca aagaacagaa cttagataga caactgaaag
      601 cgttagaggg cgtttctaag gtcttttcag acaaattaag cggtcaatcg gtcgaacgcc
      661 cacaattaca agctatgctt aactatattc gtgaagggga tatagttgtt gttactgaat
      721 tagatcgatt aggacgaaat aataaagaat taacagaatt gatgaatcaa attcaaatta
      781 agggggcaac cctggaagtc ttaaatttac cctcaatgaa tggtattgaa gatgaaaatt
      841 taagacggct gattaataat ttagtgattg aattgtataa gtaccaagcg gaatctgaac
      901 gcaaacgaat taaagaacgc caagcccaag gaattgaaat tgctaagaaa aaaggaaaat
      961 tcaaagggcg acaacctaaa ttcaaagaaa atgatccacg tttacaacac gctttcgatt
     1021 tatttttgaa tggttgttcc gataaagaag ttgaagaaca aactggaatt aatcgccgaa
     1081 cgtttagaag gtatcgatca agatacaacg tgacagtaga tcaaagaaaa aaacaatgaa
     1141 aagagggata gttaatgagt acggttattt tagctgaaaa accaagccag gcattagcct
     1201 acgcaagtgc tttaaaacaa agcaccaaaa aagacggtta ttttgagatc aaagacccac
     1261 tatttacaga tgaaacgttt atcacctttg gttttgggca tttagtcgaa ttagcagaac
     1321 caggtcatta taacgaaaag tggcagaact ggaaacttga atctttgccg atttttcctg
     1381 atcgatacga ttttgaagtt gcaaaagata agggaaagca gtttaaaatt gttgctgaac
     1441 ttctcaaaaa ggcaaataca attattgtcg caacagatag cgacagagaa ggtgaaaata
     1501 tcgcctggtc gattatccat aaagcaaatg ccttttctaa agataaaacg tataaaagat
     1561 tatggatcaa tagcttagaa aaagatgtga tccgaagcgg ttttcaaaat ttgcaaccag
     1621 gaatgaatta ctatcccttt tatcaagaag cgcaaacacg ccaaattgcc gattggttga
     1681 tcggcatgaa cgcaagccct ttatatacct tgaatttaca acaaaaaggc gtacaaggta
     1741 cattttcatt aggacgtgta caaacaccga ctttatatct catttatcaa cgccaggaag
     1801 caatagaaaa ctttaaaaaa gaaccttttt tcctgaataa ttcatagttt tactaatagt
     1861 ctttcctaac attgatttaa tagtctttaa agtattctgc ttcatcaccc attaggtgat
     1921 gaaaaaagaa cccctttctg atttagcgga caaatacttt gacctaatct cagaagatat
     1981 aaaagaaaaa ataataaaat atccgattta tactttgtat aaagaactaa agtagttagc
     2041 aaatcattta cagcaaatca ctatgttcca cataccattt tacatactta ccttatccac
     2101 gacacatgtt gagatagtat cgaagataaa gaaagttgct ccatgttgtt tttgcgagat
     2161 ggggaaatca aagccacatt gacaaccatg atgaataagc ttgctttcag tcataagcta
     2221 atcctagaac cacttttcaa gtcagttagc caaatcgatg aagaaagcga ccgtgaaaga
     2281 atggacgcta ttgataagct aatggagcaa ctattggaag aacgcaatac ccttattgca
     2341 ctgatgtcca aaggtttcct agaacctgct ctttttaatc aggaacgaaa tgttttagat
     2401 agtgagatta aaaatcttac taccgaaaaa acaaacctgg taacgaactc tgcgagtggg
     2461 gttttgcgag caaacgagat aaaggacctc attaattatg tgtcagcaga taattttaat
     2521 ggtgactaca cggaagaact atttgaagaa tttgtagtga acatcattgt aaattccagg
     2581 gatgagctga cattcaattt gaaatgcggt ctttccctga aagaaaaggt ggtgagataa
     2641 atggcatata ttccatatgg atacaaaatt caagatggag tggttactgt cgatgaaaag
     2701 gcagcaggtc aagtaaaggt attctttgag aaatacatat caggactatc ccttacagtg
     2761 gctggcgaac aggcaggtat tgaaaagaca cattctgtga tggggcgcat tttgaaaaac
     2821 gtcaactacc ttggaaatga tacgtatcca gcaattattg ataaagagat atttgataaa
     2881 gctgaagaag ttagagataa acgtgcaaag gatttaggac gagtggtaga gcttgccgct
     2941 ttcacctctc cccctcccaa agaacgattc aaaatgaaaa aggcagataa taagatgcca
     3001 gtggatcctt ttgaacgagc agaatactta tacagtctga tagaaagcga ggaataaagt
     3061 gacagagaaa aatataatgg ttattcctgc tcgtaaaaga gtaggaagta cagccgcaaa
     3121 agaaaagata aagaaacttc gtgttgctgc ctattgccgt gtttctacag aaacagaaga
     3181 acaaaattct agctatgagg ttcaggtctc acactataca gagtttataa agaaaaataa
     3241 tgaatgggag tttgctggca tctttgcaga tgatggtatc tctggtacga acactaaaaa
     3301 gcgtgacgaa tttaatcgca tgattgcaga gtgtatggac ggtaacatcg acatggttat
     3361 tacaaaatcc atcagccgat ttgcgcgtaa caccctagac tgccttcaat acattagaca
     3421 gctcaaggat aagaacatat cagtttattt tgagaaagag aacatcaaca ccacggatgc
     3481 caaaggtgag gttttgctga ctattatggc atctttggca cagcaggaaa gccagagcct
     3541 ttcacaaaac gttaagcttg ggctacagta ccgataccaa caaggaaagg tgcaggtcaa
     3601 ccataagcga tttatgggct acacgaaaga tgaagatgga aatttaacta ttgtccccga
     3661 agaagctgag attatcaaac gcatctaccg agaatacctt gagggtcaga gtctagtggg
     3721 cattggtcga gcccttgaaa aggatggtat tttaacagca gcgggaaaac caagatggcg
     3781 accagaatca gttaagaaga tacttcaaaa cgaaaaatac atcggagatg cccttctgca
     3841 aaagactgtc acagtagatt ttctgaccaa gaaacgagtg aaaaatgaag ggcatgttcc
     3901 ccaatattat gttgaaaata gccatgaagc gattattcct aaagagttat ttttgcaggc
     3961 gcaagaagaa attcatcgga gaagcaatat ctacacagga gaaggcaaga acaaaagaat
     4021 ttacagtagc aagtacgctt taagtgccat caccttctgt ggagattgtg gcgatattta
     4081 tagacgggtc tattggaata tccacggaag aaaagaattt gtctggcgat gcgtgactag
     4141 aatcgagcaa ggtcctgaag tttgtaagaa ccgaaccgta aaagaagatg aactttatgg
     4201 tgcagtaatg actgcgatta ataagctact tgcaggtggg aacaatatga taaagacact
     4261 ggaagagaat attcatgctg tgattggcga aaccacagaa taccaaattt cagagattaa
     4321 caactcacta gaggaaaaac aaaaagaact aatcaagctg gcgaacaagg gtcaagacta
     4381 tgaccactta gcagatgaga ttgatgagct gagagacaag cgacagatcc ttttagtaga
     4441 agatgcctcc ctcagtggcg agaacgagcg aatcaatgag ctgattgaat ttatccgcaa
     4501 gaacaaattc cgcaccttag aatatgatga taagcttgta aggaagataa tccagagcgt
     4561 tacagtctat gaaggccact tcgtcatagc cttcaaatct ggcatcgaaa tggaaatatg
     4621 aaaaccagaa gtaaatagcc catgactcta cagtagagtt gtgggttttc ttttgcttgc
     4681 tgattataat aatattttaa taactattgt gtttatcaac caaatggttt ataatattct
     4741 tagcgtaaac ttggaggtgc tgtatgacta cggcagaaat gattaaagaa ctgtgtgagc
     4801 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
     4861 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
     4921 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
     4981 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
     5041 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
     5101 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
     5161 agcgattaat caaattgcta cagagttgga tgatgagaat agaaacaacc ttgttgtacc
     5221 tcccggagcg tggggtgatt gggttaatgg cggtggatgg ttagttataa acgggtgtca
     5281 tgttgacttg atattacgtg atataaaacg ggtggaacaa ataatcaaag atacggagca
     5341 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgctatgta
     5401 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
     5461 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
     5521 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggcag aggataaata
     5581 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
     5641 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
     5701 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
     5761 ttttgaatgc tacgacatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     5821 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     5881 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     5941 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     6001 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     6061 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     6121 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     6181 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     6241 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     6301 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     6361 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     6421 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     6481 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     6541 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     6601 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     6661 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     6721 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     6781 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     6841 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     6901 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     6961 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     7021 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     7081 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     7141 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     7201 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     7261 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     7321 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     7381 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     7441 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     7501 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     7561 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     7621 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     7681 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     7741 ataatatatc tttactacca agacgataaa tgcgtcggaa aagttaaact gcgaaaaaat
     7801 tggaaccggt acgcttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc
     7861 ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga
     7921 ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgtggt
     7981 ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa
     8041 gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg
     8101 ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa
     8161 tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa
     8221 aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc
     8281 tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa
     8341 aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac
     8401 ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag
     8461 agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc
     8521 tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag
     8581 ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag
     8641 aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg
     8701 aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg
     8761 gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca
     8821 ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat
     8881 tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg
     8941 atgaattgtt ttagtaccta gatttagatg tctaaaaagc tttaactaca agctttttag
     9001 acatctaatc ttttctgaag tacatccgca actgtccata ctctgatgtt ttatatcttt
     9061 tctaaaagtt cgctagatag agttctatat tagagatgta aagagttttg gtgaagaggt
     9121 ttgtgagtat ggcttttata accagggtgc aatgagaagc actaacagca gctagcttaa
     9181 gaacgcatag gatatacttt ttatggaagt cacagaggag gaataaaatt tgaacaggat
     9241 taacagagta acctctattc ttattcagct gcaatcaaaa aaaataattc ctgccaaaga
     9301 aattgcacag cgatttaata taagcttaag gacagtttac agagatatcc ggacacttga
     9361 agaagccgga ataccaattg gatctgaggc cggaaaagga tattttcttg tagagggctt
     9421 cctacttccg ccggtaatgt ttacagcggc ggaagtgggt gcattgatta cagcagggaa
     9481 atttttaaat tgccatggag atgaatcatt tataaaggat tttgattcag ctatgtataa
     9541 aatcaaatct attttgaagc atggtgaaaa aaactatgca caggagttgg agaacagtat
     9601 taacgtgtac agcacatctg gacagaaaaa tacattggcg gataacgtta ttgcagcaat
     9661 tcagactgca atctgcaata aaagggtaat atcaattcaa tatcctgcat caggagggca
     9721 agaaccggaa agcagaatga tagagcctat atcactgggg ttttatgaac agaactggta
     9781 cttaatcggt tttgcaggct aagaaatgaa tataggaatt ttcgtgtgga tcggattaaa
     9841 agcatttgca tagaagaaga gctctgtcaa agccatgatg gctctttgga acaaatatta
     9901 aaacaaatgc tttcatataa aaaactatat gccctacaat gccgttgtaa aaattgggga
     9961 aaagggatat attgatttta tatctttctc aaaataaata taaaagccct ttaaagaggg
    10021 cttttatata ttaatcacaa atcacttatc acaaatcaca agtgattaat cacaaatcac
    10081 ttgtgat
//
LOCUS       DACCYH010000263         7943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.guided.118, whole genome shotgun sequence.
ACCESSION   DACCYH010000263 DACCYH010000000
VERSION     DACCYH010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.guided.118"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            128..1564
                     /locus_tag="IVY85_003117"
     CDS             128..1564
                     /locus_tag="IVY85_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8046041.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4185
                     /locus_tag="IVY85_003118"
     CDS             1753..4185
                     /locus_tag="IVY85_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP8046042.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            4415..5482
                     /gene="rlmN"
                     /locus_tag="IVY85_003119"
     CDS             4415..5482
                     /gene="rlmN"
                     /locus_tag="IVY85_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP8046043.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5675..6778)
                     /locus_tag="IVY85_003120"
     CDS             complement(5675..6778)
                     /locus_tag="IVY85_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8046044.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7330..7878
                     /gene="aac(6')"
                     /locus_tag="IVY85_003121"
     CDS             7330..7878
                     /gene="aac(6')"
                     /locus_tag="IVY85_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP8046045.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2658 a         1357 c         1724 g         2204 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaagttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaata tgagaagaaa aaagcggtga gccagcaaag ggaaaatact
     2521 gctgatgaat ggcaggaacc agaaaataca gagccggagc agctaagttt tgttccaatc
     2581 gacagttttc aagaaaatat ccatcctgcg aatctagaga aaccagtacc agatacacca
     2641 aaacagacca atacagcaga aggtttttca gatgagttgc cagaggatga cggtacctca
     2701 ttagaatttg agattgaagc agaacaggaa aatcaagact atgaattacc gacagttgat
     2761 ttgcttgatt ctataccaac tgtggatcaa agtgatgagt ataaaaaaat tgaaaaaaat
     2821 atcggcgtat tagaacaaac tttccaaagt ttcggtgtcg atgccaaggt cgtcaaagcc
     2881 agcttaggac catccgtcac aaaattcgaa gtccagccgg ctgtaggggt aaaagtaagt
     2941 aaaattgtga acttaacaga tgatatcgcg cttgctttag cggcgaaaga tgtccggatg
     3001 gaagcaccga ttccaggtaa atcactgatt ggtattgaag taccaaatgg aaagatcagt
     3061 atggtttcct ttagagaaat catcgaagca caaccaaacc acccagataa attactagaa
     3121 gtacctttag gacgtgatgt ttctggacgg gtacaaacgg ctgatctttc gaagatgcct
     3181 cacttactcg tggctggttc gactggtagt gggaagtcag tagcaattaa tgggattatc
     3241 acaagtatat taatgcgtgc taagccccat gaagtaaaac tgatgatgat cgatccgaaa
     3301 atggttgaat tgaatatgta caacggcata cctcatctgt tgacgccggt cgtgacgaac
     3361 cctagaaaag cagcgcaagc cttacaaaaa gtagtacaag aaatggaaga acgatatgaa
     3421 aaattcgcgg caaccggagt aagaaatatt tctggttaca atgaatttgt tcaacagaaa
     3481 aacttggaaa acggaacgaa acatccaacc ttaccattca tcgtagtgat cgtagatgaa
     3541 ttagctgatt tgatgatggt agccagcaat gaagttgaag atgcgattat tcgtttagcg
     3601 caaatggctc gggcagcggg gattcatatg atcctagcta ctcaacgtcc aagtgtggat
     3661 gttatcacag gaatcatcaa agcaaatgta ccttccagaa tggcatttgc ggtatcaagc
     3721 ggtacagatt ctcggacgat cattgattct aatggggcag aaaagctctt agggcgagga
     3781 gatatgctat tcttgccaat gggtgagaat aaaccgatcc gtgtgcaagg agcattcatt
     3841 tctgaccacg aagtagaacg ggtcgttcaa tttgttacag atcagcaaga agctcattat
     3901 gaagagaaaa tgatgccgac agatgaagtt gaaacgacag gtgctccaga acagccacaa
     3961 gatgagctgt ttgaagaagc aaaagcactt gtcgttgaaa tgcaaacggc aagtatttcc
     4021 cttctccaaa gaagatttcg gatcggttac aaccgagcag cacgtttagt agatgaactg
     4081 gaagctcatg gagttgttgg cccgtcagaa ggaagcaagc ctcggaaagt attcattgag
     4141 caggaagaaa catcagaaac gttgccggac gataatgaca tgtaaaactt gtgtgatgaa
     4201 gggttgaaaa acgaataacc aataaaaaga caaggcatga tgataccaaa agaaaaaatg
     4261 gtttttttcc atcttttcta atacggtatg atgtcttgtc tttttagttt ttgtgagatt
     4321 aaagttctat tcgatttatt tgtgaagtat tgcattaagg aaatgaatca tgtatagtat
     4381 gtagcgaaac tttgagaaaa agagggaaaa tactttggaa aaacagtcca tttatggctt
     4441 gacgaatgaa gaattaatca attggtttat agaaaacggc gagaaaaaat tccgagctgc
     4501 ccaagtttgg gaatggttgt atcaaaaacg tgtaagtaat ttcacagaaa tgacgaatct
     4561 atcgaaacaa ttgatcgaaa aattatcgga gcattttatc atcaatcctc taaaacagat
     4621 ggtcgtccaa gaagcttcgg atggtacagt aaaatatttg tttgaactcc ctgataaaaa
     4681 catgattgag actgtactga tgagacaaga atatggcttg tcagtttgtg tgacgacaca
     4741 agtaggatgt aatatcggct gtacgttttg tgcaagcggg ctgctcaaaa agaaccgtga
     4801 tctgactgct ggagaaattg ttgcacagat catgatggtc caacattatt ttgatgagcg
     4861 caatcttggc gaacgcgttt cacatgtcgt tgtaatgggg atcggtgagc catttgataa
     4921 ttacgacaat gtaatggact ttttgcatat catcaatgat gccaaaggat tggcaattgg
     4981 tgctcggcac attacagttt ccactagtgg attagcacac aaaatcaagg aattcgctaa
     5041 taacggcttg caagtcaatc tggctatttc cttgcacgcg ccgaataatg aggtgcgaac
     5101 atcgatcatg cggatcaacc gaagtttccc aattgagaaa ttgatggaag ctgtggatga
     5161 gtatttagag aaaacaaacc gtcgaatcac gtttgaatat atcatgttga atcaagtaaa
     5221 tgatagacca gagcatgcgc aacaattagc agatctatta aaagataaga aaaaattagc
     5281 atatgtcaac ttgattccat ataatccagt cagtgaacat gatcaatatt ctcgcagtcc
     5341 aaaagctgat gtattgagat tttatgatgt attgaagaaa aacggcgtaa actgtgtgat
     5401 acgtaaagaa catggtacag atattgatgc agcttgcgga caattgcgaa gcaagcaaat
     5461 gaaaaaaaca gttgccaaat aatcgtatcc tactcgtttt ttcaattacc ataaaaggag
     5521 cagttttctt aattaaggaa ctgttcaact taaataagag aaaagattct atagcgaaag
     5581 ctataaagct ttttttgaca agaaaaaccc tcatcaagca aagaaatgtc ctcaatttga
     5641 tgacagcctg cttgatgggg gttttagcaa ttatttaact gattttctta tatccttgct
     5701 ggctttcttg tgtagcatga taagtttcta cgtcaagaat ggcctgttta aatttttctc
     5761 gggtgatttc ataaggcaca tgtttaatat catttgttgc catggcttta tctaagattg
     5821 cttctaaatc ggtcgtgaca ttgagattca tgtcggttaa cgatactgga agttggttct
     5881 ttttcataaa cacatagatt ttatcaaaca aatcgaactg ctgatccatc atcaataagg
     5941 tcagtacacc ataactgaca gatgctccgt gaaggtatcc ctccattttt tctagaacag
     6001 tcgtaccgta atagaatgag tgtgccatag cagaattata gttattgtta actaaaacag
     6061 aaaccaatcc ggtagtaaca atgatatccg aaacgacttg ttcaattgca tatgagggac
     6121 gatcgtcggc actatctttc aatgcttgcg taccataagt caagagacga tcaatgcaca
     6181 tttttccgat ttggattcct aatctatccg tgtagcctag atcatcgcca cgagcagaaa
     6241 aagtagattc gacttctttg gataaagcat cgccaatacc tgcccataag tatttatcag
     6301 gtgcttcggc aataattgct gtatcaatga aggtgtgtac aggtggtttt gaaggaaaga
     6361 agagtccgtc cattacgcct tcttcgttat agatgacaca taccgcagtg acgggcgaac
     6421 agttcgaacc aatagtggga aaggtgaaga ctggtttagc tagtttttca ccaatgactt
     6481 ttacagtatc gcaagcacgg ccaccaccga ctgcaaacag gacgtctgct ttttggacac
     6541 tttcatgctg gatcaactgt tcaatatttc cataagttga attacccccg taccagagac
     6601 tggcgacaac ctctatttcc tgtgtcaaag ctacttttat tttgttttct gcctttgaaa
     6661 gtgcagtctt ccctccaatc agcactactt ttgtcccata ttctttgacg attccatttg
     6721 ctttttcata cgcatctgtt ccaatggtat aatttggtaa gaaaacactt tgactcatat
     6781 tcatttcctt ctttcttcgt tgaaacgggt taaaatttta aagagtgatc gatctgactg
     6841 cggctgtaga tggagtagag gtgaacctaa gacaggataa gtgatgaata aggtggcatg
     6901 aacaccttga aatcatcaat aagtatccta aaaacgaaca agtacaaaaa cattgaaaac
     6961 gacaataatg aatttttgtt cctcttagta tttctaccga ttccctttgc ttttttgtaa
     7021 ttatctaaaa attctaaaaa ttaagattat tatatgaaaa caggttttat ttgtcaattc
     7081 ataattggtt ttttttaaag caaaagataa tctatatcat gatgaacaaa cagagttttt
     7141 tcctccaatt gatggctttc ctgatctttc ctattttgaa taaagtgttt ctgtataatt
     7201 ttttatttta taagtaagat aaataatgaa agaatagaga tactgattgg tagctaaaga
     7261 tttttgagcg agaacatttc gttttttcaa aaggactgtt agaataagca agaacaaggg
     7321 ggaagacgta tgataatcag tgaatttgac cgtaataatc cagtattgaa agatcagctt
     7381 tctgatttac tgagactgac ttggccggaa gaatatggag acagctcggc agaagaagta
     7441 gaagaaatga tgaatccaga acgaatcgcg gtagcagcgg tagaccaaga tgagttagta
     7501 ggatttattg gtgcaatccc tcaatacggt atcacaggtt gggaattgca tccattagtt
     7561 gtagaaagct cccgacgaaa gaaccaaata ggtactcgat tagtcaatta cttagaaaaa
     7621 gaagtagctt ccagaggagg aatcacgatt tatttaggta cggatgattt agaccatgga
     7681 acaacgttaa gtcaaacgga cctgtatgag catacatttg ataaagtggc ttccatccag
     7741 aaccttcgtg aacatccgta tgaattctat gaaaaattag gttataaaat cgtaggtgtc
     7801 ttaccaaatg caaatggctg ggacaaaccg gatatttgga tggcaaaaac gattattcct
     7861 cgaccaaatt ctcaataata aaaagaggtt gtgacagaag taatcagctt caagaaataa
     7921 gaaggaattt acgaaaatta ctt
//
LOCUS       DACCYH010000264         5849 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.guided.136, whole genome shotgun sequence.
ACCESSION   DACCYH010000264 DACCYH010000000
VERSION     DACCYH010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.guided.136"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            322..1014
                     /locus_tag="IVY85_003122"
     CDS             322..1014
                     /locus_tag="IVY85_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8046046.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1341..1838
                     /gene="dfrG"
                     /locus_tag="IVY85_003123"
     CDS             1341..1838
                     /gene="dfrG"
                     /locus_tag="IVY85_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP8046047.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1910..3862)
                     /locus_tag="IVY85_003124"
     CDS             complement(1910..3862)
                     /locus_tag="IVY85_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP8046048.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3869..4165)
                     /locus_tag="IVY85_003125"
     CDS             complement(3869..4165)
                     /locus_tag="IVY85_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8046049.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            4507..4797
                     /locus_tag="IVY85_003126"
     CDS             4507..4797
                     /locus_tag="IVY85_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8046050.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            5511..5813
                     /locus_tag="IVY85_003127"
                     /pseudo
     CDS             5511..5813
                     /locus_tag="IVY85_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1996 a          925 c         1010 g         1918 t
ORIGIN      
        1 tttcataggt aaaagaagca tgacaattta tgacaaatcc gcgttttttg taataaatac
       61 agtaatctat gaaatgaaaa gggttacgtt taataaaact tgcttttttg tttttgttca
      121 ccataaatat ttatttaaga aagtattaac ctgcattttt ctctataaaa aagatttttt
      181 aaatagataa aaaaggagga ttaaatgagt atttgatagt atttatatta aaattacata
      241 tgtaatttaa aaacgcttga cattgtgttt gagatcactt acttttagca tgtaaaagta
      301 ttggattaag gagctatgca aatgaaaaag aaatttatta ctggtgggat tgtcgttgca
      361 tttctatgtc taattggcta cacaggcacg cggtacgcaa atgaaattat tttgtggggt
      421 ggagagacag atattgatac aatcaatgag aatctgacac agcttgatca tacattgaca
      481 gataaagagc aagcgattac agatcttacc gctcgactag cacaaaaaga acagacattg
      541 caagattcaa aggcagatgt ccaagaatac caaacaaaag taaatgaatt agaaaaccaa
      601 aaaaatcaat cggcagcaga aaaacaaaac ttagaaagtc aattagtcag caaggatgca
      661 caactacaaa gcaaacaaaa tgaaatcaat gtcaaactag atgagatcaa ccaaaaaaat
      721 gatgaaatca atcatctgaa tgcagattgg acagcaagac tacaagaagc acaagcaaaa
      781 ctttcagaat tacaaaatac tgtaaatggt ttgaatcaac aaatcgcaac actgacacaa
      841 gaaaaaacaa atgtagaaac tcagttaaca gatacacaaa agaaattaga cgaagcaact
      901 gctgaaaact caagcttgaa acaatatatt caaaagttag aaaaagcaaa agatgaagtg
      961 gaagatacag ctgataaatc tcagcaaatt gttgaagaac atttgcgtaa ataatctttc
     1021 ttaacaagta aacctaaatt agtaaagaag caacagtaat ctatactaag tagttcagtt
     1081 ttggagtaca aaatgacaaa atgtacttca aaatggacta cttttttcat tttaacttac
     1141 ataccaaatt acatacaaaa tgatttataa aatgcaattg aaaatgataa ttgaatgaaa
     1201 atttgtgatt ttgtggtatt atgataatat aacttataga aaaaattgct tattcaacta
     1261 acgggcagga taatgagaaa aggttttttg atagaaataa gaaattagat ggtaaattag
     1321 ttaaaaaggc tgtgttataa atgaaagttt ctttgattgc tgcgatggat aagaatagag
     1381 tgattggcaa agagaatgac attccttgga ggattcccaa ggactgggaa tatgttaaaa
     1441 atactacaaa gggacatccg ataatattag gtaggaagaa ccttgaatca atcggaagag
     1501 ccttacctga cagaagaaat attattctga cgagagataa ggggtttacc tttaatggtt
     1561 gtgaaattgt tcattcaata gaagatgttt ttgagttatg taaaaacgaa gaagaaattt
     1621 ttattttcgg aggagaacag atttataatt tgtttttccc ttatgttgag aaaatgtaca
     1681 tcacaaaaat acatcatgaa ttcgaaggag atactttttt tccagaagtg aattatgagg
     1741 aatggaatga ggtatttgcc caaaaaggga taaagaatga taaaaatccg tataactact
     1801 attttcatgt atatgaaaga aaaaacttat tgagttaacg ggtgcgttaa ttgaagaaga
     1861 acagtaaaga taaagcacaa gagcttggga ttactccaag ctctttttgt catttgaaat
     1921 aaattatatc tttccaatca aattgtttat ctgtaatccc taatctttga gcaggtgtca
     1981 aaaccttttt atccgctgat ttaaaagggc gacaaaagtt gtagtaagtt cttagaattg
     2041 taatcgcaaa ttgagcatac ttcggattga agttagcgta aatatagctc ttcttatcac
     2101 cacgagctgt tgttaatggt ctttcaagag atgatatata acgtcttatc tgttgaataa
     2161 agctattggt cgaatggtca ttaacattta atagcatctt tgcgatttcg tttggttcca
     2221 gagcagataa atccgttcta caatcaactg aataaaatcc tttatcctta gtggctaatg
     2281 gatgtttgat agggttatcg gcatatttgc gatatctttc tccatcttta ctaatagcct
     2341 cttcgtggaa ttgatgactt gttagtaatt cagttagata atgagatgcc aaagtccgta
     2401 gaaactttgt tttgaatcct ctgatatctc cccaatcgag cagttctgct tttgcttgtc
     2461 cgaattcttt taagcattgt tttctgcttt tcgtcttgtt aacttggcaa ataaaatgat
     2521 gtgcatcgct caaccttaat tctttcgtga atactctata aaaggcgttt ctaatagaga
     2581 agtcatcatc actaatcatt cgccattcgt cggcattaac catttgttta attaaccaat
     2641 agtgagcagt tgtagtataa ggagcatcaa catttaaacc atcgacaaat ctactcctat
     2701 tagtaatttt ccctaattcg tgaagataag cattcctatt ttcgctatcg ttagcggatg
     2761 gttcttgtgg ataataagac caatctaacc tatcattttt acgactaaaa gtattgagat
     2821 ggtcttcttt gaattttctt gtatcttcat ttaattcatc cattgaaata ttccaatcat
     2881 aggcaacatc agaacggaac acatatcttg ataagacctc agcagataca actatatacg
     2941 tttgcatatt aaggtcttca aaccccgtat atttctttga accttgtcct ttcttacgga
     3001 cgttatttag gtagtagtgc atcttgtccg tattaagcca catctcgtta aatttctttg
     3061 tttgaagggg ttgtgtctca tatcgttcta aaaactccaa acaacgccta tacagccact
     3121 ccagcttatg atagtaggtt ccaactccaa ttcctaaaat atcacaagta cgactgactg
     3181 aaacccttcc aacaaccatc ttagcgaaca ttggaagaat ggtgttcttt tgttggtgat
     3241 aagtagtggt ttcttctctt tttggtagaa cattagtgaa cttcttacac gccttacatt
     3301 gatagcgttg agatttgccc ctacttttcc cccttttgta aaattgcttc ggctcattga
     3361 atggggttga ttcttcttcc gagcagcctt ctttatgaaa ttgataatct ggctcaacat
     3421 cttggatgct attgatttct ataaggcgtt taatttcttc aacaacagac caattagata
     3481 aaggggttac tgtacagttt tgcgatacac ctctgccaat aggattgtca ttgcagtaca
     3541 tacctttatg acctttatct ttggaactac cagtcatttt atatcgactt ggtttccctt
     3601 ttacatcttt gaatttgtgc tgttctttcc caaaattaca acaaaacgga ttgatgcagt
     3661 aattatattg aatgggatat tcagaaccgt tccatttaaa agacgttggc aagaacaaca
     3721 ttttgttata ccttgttgca aatcgttttg gagttaataa atcatattgt ttagccctat
     3781 cttttaattc ttcgtttgaa atcggaattt taacatctac aatttcatct tccttcgtgg
     3841 ctaatctttt aagcttggtc attgtctatc aacccactct atctttgata cccatttttt
     3901 caacaatccg cttattactt cgcttgttgg caatccattc aataaacttc ttatcgtcta
     3961 aaatgttcaa taagaattca tccactgctt cgctttcagt acgctctgca tattcagcgt
     4021 attgcttaac tatcaccctt acttgctctg atatttccca atccacagac ttagcgttct
     4081 tattttttgg ctcaagaaat tgcataggaa aatgcacccc tttttgctta gtaaattgta
     4141 accattttaa cacaccaatt tgcattccta caataattat ttcacgttcg catttttata
     4201 tacattttaa cttacatttt gtggtgtaaa ttaacaacaa acgttgattt aacaataaaa
     4261 aacgagtatc aaattcagtt gcaatttgat actcatttag gtttacattt tcactttttg
     4321 tactccaaaa ctgaactact taaatctata ctgctgcctc ttttttttag tacatgtcct
     4381 tcatcttcgc aggaagtgcc aatatgattt taagaaaggt aatagacaaa aagtcaaaaa
     4441 gataaaacag agtttcttct ttacaaaggg aatgtatgtt cgtataatgg tattgaggtg
     4501 aaggttatgg agaaaaaata tggaacagta gataagatcc gtactttgca agttagtgcc
     4561 agaccgttga tccgtttcag tttaaatggg gtcaattgtt tgattgcttc ccatagtttg
     4621 aacttcctag ctgaagtaga cgaagggatg aagttagtcg ttacaggtta ttataataat
     4681 agaaagcaat ttgttgtgag atcctatcat gtgttaggga aacccaaaat tgtggtagaa
     4741 tatgagaaaa gtctataccc aagaaaaaag gtccgacaag atgaaagaat aaactagaaa
     4801 acaagcggct gagacaaaaa tgtcctagcc gcttgttttg gaaagatgga tgcagacgat
     4861 gggatttgaa cccacacgag catacgctca caagaacctc aatctatata taactacatg
     4921 tatttaagta tttgtgatga atcactaaac tttccaaact catttgaggg atattgctta
     4981 aagattgaac agatcaattc gaagccctat tagtattcgc tcaatggggg gatcagaggc
     5041 cataaaaaga gactagtcat tactagtctc aggtagtgga attgtaggat tttaaacgtt
     5101 cctggagagt accggtatgt aactcgaaga ggtgattatc atagtcgtaa aagtaaattg
     5161 aagaagcctc gcctttcaca cgagaacgtc ctggttcgac agttaaacct aacatttgga
     5221 tttttgagac aaacgaatct atatcttgtt cgtcaatttt aaaagcaata tgattgtatg
     5281 tctttggcag tttattcgac gaattctgca ttactgcaat ccataggtcc ttaactaaga
     5341 aaaatttttc tggatataat gaaaattttt ttgttgacta tcgtagattt cttttgcatc
     5401 aaataaggta tttaacaatt aagtatttaa aagataactt ttaagttatc tttaagtaat
     5461 gcttaaagct atgttatact ctattaggaa atataagaag gagtataaaa atgaacaaac
     5521 ataatagaaa aataacgaat aacaaagaac tgatcttgtt actaataggg atttggggta
     5581 tctttttact ctttaatatg tatatgccga ctatgagagc ggatgacgta gtttatgcaa
     5641 gtagattaga tgaacttggc tatttgggtg cttcgataga acattataag acatggagtt
     5701 caagaattat tatagaacta ttcttaatgt tcttttctaa acactttatg ctatggaaat
     5761 tattaaattc tacgattatg ttaggaattg tagtattact ttgcaagtat gttgagagta
     5821 taaaatattt tgtgtaaatg aaaaaatcc
//
LOCUS       DACCYH010000265         4819 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.guided.146, whole genome shotgun sequence.
ACCESSION   DACCYH010000265 DACCYH010000000
VERSION     DACCYH010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.guided.146"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            191..997
                     /locus_tag="IVY85_003128"
     CDS             191..997
                     /locus_tag="IVY85_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HAP8046051.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            1015..1842
                     /gene="vanW"
                     /locus_tag="IVY85_003129"
     CDS             1015..1842
                     /gene="vanW"
                     /locus_tag="IVY85_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAP8046052.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVFADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            1839..2810
                     /locus_tag="IVY85_003130"
     CDS             1839..2810
                     /locus_tag="IVY85_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP8046053.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2803..3831
                     /gene="vanB"
                     /locus_tag="IVY85_003131"
     CDS             2803..3831
                     /gene="vanB"
                     /locus_tag="IVY85_003131"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP8046054.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            3837..4445
                     /locus_tag="IVY85_003132"
     CDS             3837..4445
                     /locus_tag="IVY85_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP8046055.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT         1360 a         1016 c         1276 g         1167 t
ORIGIN      
        1 agaatgattt tctatcagtc ctagaaatta tagagccgat tttgaccgac aggtataacc
       61 ctgccggtct ttttttgctg ctacaggaaa actacagact ggctacaggg aaagtacaga
      121 tgcgcttgcc ataataacaa tcgtaccagc cacaaatcgt agttttattg caaaggaggc
      181 attcgatcaa atggaaaaaa gcaactatca ttccaatgcg gatcatcaca aacggcatat
      241 gaaacaatcc gtggaaaagc gggcttttct atgtgcgttc attatttcgt tcacagtctg
      301 cacgctgttt ttggggtgga gactggcttc cgtattggag gcaacacaga taccgcccat
      361 ccctgcaact catacaggca gcagcactga cgtagtggag aatttggagg aaaacgctct
      421 tgccaccgcc aaagaacagg gagatgaaca ggaatggagc ctgattttag tgaacaggca
      481 gaaccccatc cccgcacagt acgatgtgga gcttgagcaa ctatcaaatg gtgagcggat
      541 agatattcgg atttctccct atcttcaaga tttgtttgat gccgcaagaa ctgatggagt
      601 ttacccgatt gtcgcatccg gataccgaac aacagaaaaa cagcaagaaa ttatggatga
      661 aaaaattgcc gaatataagg cgaaaggcta cacctctgca caggctaaag cggaagcaga
      721 aacttgggtg gccgtgccgg gaacgagcga gcatcagctt ggtcttgctg tggatatcaa
      781 tgcggacgga attcattcaa caggcaacga ggtttataga tggctggatg aaaacagcta
      841 tcgttttggt tttattcgtc gctacccacc agacaagaca gagataaccg gtgtgagcaa
      901 cgagccgtgg cattaccgat atgttggcat cgaagctgcc acagagatgt acaaccaagg
      961 ggtttgcctt gaggaatatt taaaaccaga aaaatgagaa aaggatgtta tgttatggac
     1021 agaaaaagat tgacacagcg tttcccgttc ctgcttccaa tgagacgggc gcagagaaaa
     1081 atgtgctttt atgcgggaat gagatttgac ggctgtcgct atgcgcagac gataggggaa
     1141 aaatcgcttt cccatttgct ctttgaaacg gattgcgcat tatacaacca caataccgga
     1201 tttgacatga tataccagga aaacaaggtg ttcaacttaa agctggcagc aaagacctta
     1261 aacggcttat tgataagacc gggggaaacc ttttctttct ggtggctggt acgccatgcg
     1321 gacaaagata ccccctataa agacggcctt acggtgacca atggtaaact caccaccatg
     1381 tcgggcggcg gtatgtgcca gatgagcaat ttactatttt ggatgttcct gcatacgcca
     1441 ttgacaatta tccagcgcag aggtcacgaa gtaaaggagt ttccagagcc aaacagtgac
     1501 gagattaaag gggtggatgc aaccatttca gagggctgga ttgatttaaa agtgcgaaac
     1561 gataccgact gcacctacca aatatgggtg accttagatg atgagaaaat catcggtcag
     1621 gtgttcgccg acaaagagcc ccaagcatta tacaaaatta caaatggcag tatccagtat
     1681 gtccgtgaaa gtggcgggat ttatgaatat gcccaggtta aacggatgca agttgcctta
     1741 ggtaccgggg aaataataga ttgcaagctg ctttatacaa acaaatgcaa aatctgttat
     1801 cccctcccgg aaagtgtgga tattcaggag gagaaccaat gagaaaaagt gtgggcatta
     1861 ctgtttttgg atgcgaacag gatgaggcaa atgctttccg cgctttatcg ccggattttc
     1921 atattatccc tacgctgatt agcgatgcga tatcggcaga caacgcaaaa ttggccgctg
     1981 gcaatcaatg cgttagcgta agccataagt ccgaggtttc cgaggcgaca attcttgcgc
     2041 tgagaaaggt cggggtaaaa tacatttcta cccgcagcat cggctgcgat cacattgata
     2101 cgactgccgc cgagagaatg ggaatctcgg ttggcacggt tgcgtattcg ccggacagcg
     2161 ttgcggatta tgctttgatg ctgatgctga tggccatacg gggtgcaaaa cccaccatgc
     2221 acgccgtggc gcaacaagat ttcagattgg atcgtatccg ggggaaagaa ctgggggata
     2281 tgactgtggg agttattgga accggccata tcgggcaagc ggtcgtcaaa aggctgcggg
     2341 gatttggatg ccatgtgctg gcctatgata acagccgaaa aatggatgca gattatgtcc
     2401 agcttgatga gcttctaaaa aacagcgata ttgttacgct ccatgtgccg ctttgtgcgg
     2461 atacccgcca tctgatcggt cagaagcaaa ttggagagat gaagcaaggc gcatttttaa
     2521 tcaacaccgg gcgcggagcg cttgtcgata ccggggcgct ggtggaggcg ttggaaagcg
     2581 gaaagctggg tggtgcggca ctggatgtgt tggagggcga ggatcagttt gtttataccg
     2641 attgctcgca gaaagtgctt gaccatccct ttttgtcgca gcttttaagg atgccgaatg
     2701 tgatcatcac accccatacg gcgtactaca ctgagcgtgt gctgcaggat actacagaaa
     2761 aaacaatcag gaattgtctc aattttgaaa ggagtttaca gcatgaatag aataaaagtc
     2821 gcaatcatct tcggcggttg ctcggaggaa catgatgtgt cggtaaaagc cgcaatagaa
     2881 attgctgcga acattgatac ggaaaaattc gatccgcact acatcggaat tacaaaaaac
     2941 ggtgtatgga agctatgcaa gaagccatgt acggaatggg aagccgacag tctccccgcc
     3001 atactctccc cggataggaa aacgcatggg ctgcttgtca tgaaagaaag cgaatacgaa
     3061 acacggcgta ttgatgtggc tttcccggtt ttgcatggca aatgcgggga ggatggtgcg
     3121 atacaggggc tgtttgtatt gtctggtatc ccctatgtgg gctgtgatat tcaaagctcc
     3181 gcagcttgca tggacaaatc actggcctac attcttacaa aaaatgcggg catcgccgtt
     3241 cccgaatttc aaatgattga taaaggtgac aagccggagg cgggtgcgct tacctaccct
     3301 gtctttgtga agccggcacg gtcaggttcg tcctttggcg taaccaaagt aaacggtacg
     3361 gaagaactta acgctgcgat agaagcggca ggacaatatg atggaaaaat cttaattgag
     3421 caagcgattt cgggctgtga ggtcgggtgt gcggtcatgg ggaacgagga tgatttgatt
     3481 gtcggcgaag tggatcaaat ccggctgagc cacggtatct tccgcatcca tcaggaaaac
     3541 gagccggaaa aaggctcaga aaatgcgatg attacagttc ccgcagacat tccggtcgag
     3601 gaacgaaatc gggtgcagga aacggcaaag aaagtatatc gggtgcttgg atgcagaggg
     3661 cttgcccgtg ttgatctttt tttgcaggag gatggcggca tcgttctaaa tgaggtcaat
     3721 accctgcccg gttttacatc gtacagccgc tacccacgta tgatggccgc cgcaggaatc
     3781 acgcttcctg cactgattga cagcctgatt acattggcgt taaagaggtg ataagtatgg
     3841 aaaatggttt tttgttttta gatgaaatgt tgcatggcgt ccgttgggat gccaagtacg
     3901 ccacatggga taacttcaca ggaaaaccgg tggatgggta tgaggtgaat cgcatcatcg
     3961 gcacaaaggc cgtggcgttt gctctgcgcg aggcacaaat ccatgcggct gcgcttggct
     4021 atggcttgct tttatgggat ggatatcggc caagaactgc ggtggactgc ttcctgcgtt
     4081 gggcagcgca accggaggac aatctcacaa aagaaaaatt ttaccccaat atagagcgag
     4141 ccgagttgat tacaaagggt tatgtggcct cacaatccag ccatagccgt ggaagcgcaa
     4201 ttgatcttac gctctaccac ctggatacag gggaacttgt ttcaatggga agtaacttcg
     4261 attttatgga cgaacggtcg catcatacag caaaagggat aggggatgca gaggcacaaa
     4321 atcgaagatg cttgcgtaaa atcatggaaa gcagcggatt tcagtcttat cgctttgaat
     4381 ggtggcacta taagttgatt gatgagccat accccgatac ctattttaat tttgccgttt
     4441 cataatgaaa gtattggttt tgtatcagat tttggatttg ataaaatcta tgctggtcat
     4501 ggacaatctt ttactggaaa gtggttaaag catcttttgt aatacattgc aaattatagt
     4561 attcaatctg cccagcggca gaaaagaaaa aaaccgctgt tgggcagacc tttcccacgc
     4621 ccattttgta ttttgctggc ggcggttttg agaaatcaag gccgccgttc ttttctggca
     4681 gcaaaaggtg tggcggccca taggaggatg ccgctatgct gttttgctat tgccatgctc
     4741 taatccactt ccaccactca aaaaaagcct tgcccctcca tgaactgaac cccaaaagtt
     4801 gaactattta atggactgt
//
LOCUS       DACCYH010000266         4443 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.guided.153, whole genome shotgun sequence.
ACCESSION   DACCYH010000266 DACCYH010000000
VERSION     DACCYH010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.guided.153"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            153..719
                     /gene="amaP"
                     /locus_tag="IVY85_003133"
     CDS             153..719
                     /gene="amaP"
                     /locus_tag="IVY85_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8046056.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            732..920
                     /locus_tag="IVY85_003134"
     CDS             732..920
                     /locus_tag="IVY85_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8046057.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            933..1490
                     /locus_tag="IVY85_003135"
     CDS             933..1490
                     /locus_tag="IVY85_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8046058.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1522..1764
                     /locus_tag="IVY85_003136"
     CDS             1522..1764
                     /locus_tag="IVY85_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8046059.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            2105..3496
                     /gene="amt"
                     /locus_tag="IVY85_003137"
     CDS             2105..3496
                     /gene="amt"
                     /locus_tag="IVY85_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP8046060.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGFLAMTISMVVSLYEYPTFATSGFSLVFFLLLGGLLWFIPVALCAAEMATV
                     KGWEKGVSQTKRY"
     gene            3891..4364
                     /gene="dfrF"
                     /locus_tag="IVY85_003138"
     CDS             3891..4364
                     /gene="dfrF"
                     /locus_tag="IVY85_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAP8046061.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
BASE COUNT         1466 a          682 c          959 g         1336 t
ORIGIN      
        1 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
       61 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      121 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      181 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      241 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      301 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      361 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      421 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      481 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      541 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      601 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      661 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      721 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      781 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      841 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
      901 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
      961 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1021 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1081 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1141 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1201 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1261 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1321 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1381 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1441 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1501 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1561 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1621 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1681 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1741 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1801 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1861 ttctttattc cttatgaaaa aatatcttta aaaataaaaa aagacgaaaa taaacggttt
     1921 tctataaaaa gaagaccgtt tattttttgt ctttatttgt gtgaaaaacg aaaaagagca
     1981 caacacatca agcttttatt aattgtctgt cttttataaa tgcaagtatt aattcaaaaa
     2041 aacagaagaa aaaaacaact acaccttctt ttatactcgt aatcgtagaa aggaggcgaa
     2101 aaaaatgaat acattaccta tcaatacagg agatacagca ttcatgattt tatgtacagc
     2161 aatggtttgc ctgatgactc ctggtctagc ttttttttac ggcggattag cacggaagaa
     2221 taatatacta acgatcatgt ctcaatcttt tctctccgtt gggataacaa cgattatgtg
     2281 ggtatttggt ggattcggat tggcctttgg acgggatatc ggcggtgtta ttggaaaccc
     2341 ggcagatttc tttttgatga gacatgttac aagcttcccc aacatgtttc atggtgcaac
     2401 gattccattt ttaatgttct ttctttacca actgatgttt atcgtgatta ccgttccttt
     2461 gatgacagga gcttttgcag ggagattgaa tttaaaaggc tatattatac ttgttatttt
     2521 ttggaatctg ctgatttatt tccctgtttg ccactggata tggggtggcg gatttttgga
     2581 tcagatggga tttagagatt tcgctggtgg agcagtcatc cacacgacag ctggattcgg
     2641 ctctttagca tgtatcctca cacttggtca aagaaagatt cctttaggag aaagaagtca
     2701 gcatagcagt cttatggcag ctgctatcgg tacaggactg ctttggtttg gctggttcgg
     2761 ttttaattcc ggtggtgcac ttcgagcaga tgaacaagca gtcaatgcat ttggttcaac
     2821 attcatcgct cttgcatttg cgatgattac ttggctgatc attgcaaaag taaaaggaaa
     2881 tggctttgat tttgtcgacg tactaacagg atccgttgct ggattggcag ccattacgcc
     2941 ttgtgccggt tatgtagaag caaaaagtgc catgctgatt gggatcattg cgggtatcgt
     3001 ctgtcatgct gctgttgatt ttcgtaaaaa gaaacaatgg gatgatgcac ttgatgtgtg
     3061 gggtgttcat ggcatgggcg gattcacagg tacgattctg atagggatct ttgcatctaa
     3121 cagccattta ctaacgaatc caagcagctg gtacttctta gggattcaag tgattggtgt
     3181 catcattact gccgtgtatg cctatgtgct gacaacagtg attttaaaat cggcgatgca
     3241 ctttacaacg atcactacta caaaagaaga acaagaagaa ggattggact ttttcttctt
     3301 tggctttttg gctatgacca tctcaatggt cgtttcatta tatgaatatc ccacattcgc
     3361 gacctcaggc ttcagcttag tatttttctt gttattaggc ggtttgctat ggtttatccc
     3421 tgtggcattg tgcgcagcag agatggcaac ggtgaaagga tgggaaaaag gtgtttccca
     3481 aacaaaaaga tattaagaaa cagcccatgt ttacctgctt gcaaacaaaa gaatattgct
     3541 actagactaa gagatactac ataagtgtgt tggtatctct tttttgttgc cgtgaaacag
     3601 cagactattt catttctgct tgattacctt aaaacttatt agtctatggt ttgtcaaggg
     3661 cttgttgcgt gagaaatgct gaatatcacc ctttacaaaa cataggctga taagtaactt
     3721 ttcaaaagca gaaaagaaat ggcaatgctt ataacaatat gatataatgc agatgaagac
     3781 aaaaaaggaa tttgtcgagc tggttagttc gagataattt tgagtttgtt aagatgtggt
     3841 gcacttgatg gaatcacagg tacgacatca tattatggag gatgaaatag atgataggtt
     3901 tgattgttgc gaggtcaaag aataatgtta taggcaagaa tggtaatata ccatggaaaa
     3961 taaagggaga acaaaagcaa tttagagagt taacaacggg taatgtggtt attatggggc
     4021 gaaagtctta tgaagaaatc ggtcatccgt tgcctaatag aatgaatatt gttgtttcca
     4081 ccacaacaga gtatcaagga gataatttag tttcagttaa atcattagaa gatgcattat
     4141 tattggctaa aggacgagat gtatacatat ctggtggata tggactattt aaggaagctt
     4201 tgcaaatagt agataaaatg tatatcacag aagtagattt aaatattgaa gatggagata
     4261 cattctttcc agaatttgat atcaatgatt ttgaagtttt gataggggaa acacttggtg
     4321 aggaagtgaa atatacgaga acattttatg taaggaaaaa atgaattgag taaattttgg
     4381 atttagggtg ttttcataaa tatattgctt tttatgcata tataaattgt tgtaagacaa
     4441 att
//
LOCUS       DACCYH010000267         2771 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate UW13722
            SAMN11027202-rid9893793.guided.172, whole genome shotgun sequence.
ACCESSION   DACCYH010000267 DACCYH010000000
VERSION     DACCYH010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN11027202
            Sequence Read Archive: SRR10694447
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743200.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743200.1
            Genome Coverage       :: 239x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 04:24:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,143
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 3,017
            CDSs (with protein)               :: 3,017
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 31 of 63
            Pseudo Genes (internal stop)      :: 15 of 63
            Pseudo Genes (multiple problems)  :: 7 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN11027202-rid9893793.guided.172"
                     /isolate="UW13722"
                     /isolation_source="perianal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Germany: Bavaria"
                     /collection_date="2014"
                     /collected_by="Robert Koch Institut"
     gene            110..193
                     /locus_tag="IVY85_003139"
     CDS             110..193
                     /locus_tag="IVY85_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP8046062.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="IVY85_003140"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="IVY85_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP8046063.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1318..2181)
                     /locus_tag="IVY85_003141"
     CDS             complement(1318..2181)
                     /locus_tag="IVY85_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP8046064.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2183..2455)
                     /locus_tag="IVY85_003142"
     CDS             complement(2183..2455)
                     /locus_tag="IVY85_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8046065.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2472..2681)
                     /locus_tag="IVY85_003143"
     CDS             complement(2472..2681)
                     /locus_tag="IVY85_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP8046066.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          851 a          487 c          443 g          990 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa attttgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac cgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttat agatgtaatc
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt
     1081 ggaaagttac acgttactaa agggaatgta gataaattat taggtatagg gcacctctaa
     1141 taactaccaa ttaattcacc tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc
     1201 tatttcttat cgatacaaat tcctcgtagg cgctcgggac cccaacatga ggaaatgttt
     1261 ttataaaaaa agagtagtta ccgttttttg gtaactactc ttttcctttt ttaaggttta
     1321 aatacctgga agtttaggtg ttttgggtat tggtggtgta ggtagctgca agctttccat
     1381 tttttgttga attgctttaa attcaggggt ttcttggtgt tgattttgaa ccattttttg
     1441 ctctattctc tccaaagtcg gttgaatttc tttccctgat actttacgat ttaattctct
     1501 ttctaaggtc tctttcggac taatggaagg cgtttctaag cttgaataga gtttcactcc
     1561 ttctctattg taaagcctta tatcgctaaa taagcccgtt ttatgaaggg tttctaaatt
     1621 ggtcggtaag tttttgacaa caatatcatg cgcttgtttt ggtgttgccc tagctgtcat
     1681 tggatcatct gcatacatgg tttcatatcg ttcaatcgtt cctaagtatg attcaatttt
     1741 cggtactgcc atgacgtata tttttgtttc ataaccttta gattgaagca ttgtggctgt
     1801 ttgaatggga acgtctgttg ttcgtcctgt tccttcaatc actaaattat agccttgatc
     1861 gcttaaacgg ctaataagtg cttctgtcat gcgattcgaa taaggggtaa cgtgttttac
     1921 tacgtctttt tcatagagtt tcactaattc gtcaaaattt gggtgctgtt gtttaaaggt
     1981 atcattatca atgacaataa cattcccttg tgtttcttca aaaattgctg atcgcaaact
     2041 ggttttccct gaccctggtt gcccaccaag taaaaaagcg gttggcgatt caaccgctag
     2101 tctattttta gttagtcgtt caacattctt ttctaaacga tcttcaaatt gcttttcggt
     2161 gaagtcagta atattcgcca tgttaagcca ctttctcttt attcaaaatt tgttttgaat
     2221 attgattcat tgaccgccaa tactcatgaa tagcttgtaa ttcttcaaat ggttgttgaa
     2281 ataaatcaac ttcttgtgcc acttctaatt gatttaaaag attcacttct agtaaacgag
     2341 tatttttagt atctagttca tgattcaaaa cataatttaa tacccgatta taaacacttg
     2401 ccgataactc attaatgatt tcaatttcaa atgttttttc atacgtaact gccatttcat
     2461 tttcactcct tttaaagttt gtcaggtaaa tatttccgta tatattcttc taaggcttga
     2521 tccataactt cttttacgtt tccgccattc tttgctgttt cgatttttat gatatggtgc
     2581 aagtcggcac gaacacgaac cgtcttatcc cccattatat ctttttttgc gctgattggc
     2641 gtatcatttc gttttgcttt ctgtgcgcct aaatttccca cgatcgctca cttctttcta
     2701 tttcttctta ctatttattt tatcatcacc tatcacaaat cacaagtgat ttgtgattaa
     2761 tcacttgtga t
//
